LOCUS       DACEPD010000001        77359 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.001, whole genome shotgun sequence.
ACCESSION   DACEPD010000001 DACEPD010000000
VERSION     DACEPD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.001"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(524..751)
                     /locus_tag="IW797_000001"
     CDS             complement(524..751)
                     /locus_tag="IW797_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1222811.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            complement(760..1233)
                     /locus_tag="IW797_000002"
     CDS             complement(760..1233)
                     /locus_tag="IW797_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ1222812.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            2463..3029
                     /gene="amaP"
                     /locus_tag="IW797_000003"
     CDS             2463..3029
                     /gene="amaP"
                     /locus_tag="IW797_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1222813.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            3042..3230
                     /locus_tag="IW797_000004"
     CDS             3042..3230
                     /locus_tag="IW797_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1222814.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            3243..3800
                     /locus_tag="IW797_000005"
     CDS             3243..3800
                     /locus_tag="IW797_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1222815.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            3832..4074
                     /locus_tag="IW797_000006"
     CDS             3832..4074
                     /locus_tag="IW797_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1222816.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            4415..5629
                     /locus_tag="IW797_000007"
     CDS             4415..5629
                     /locus_tag="IW797_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1222817.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            5660..5878
                     /locus_tag="IW797_000008"
                     /pseudo
     CDS             5660..5878
                     /locus_tag="IW797_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6297..6770
                     /gene="dfrF"
                     /locus_tag="IW797_000009"
     CDS             6297..6770
                     /gene="dfrF"
                     /locus_tag="IW797_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1222818.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            6897..7553
                     /locus_tag="IW797_000010"
     CDS             6897..7553
                     /locus_tag="IW797_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ1222819.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            7735..8265
                     /locus_tag="IW797_000011"
     CDS             7735..8265
                     /locus_tag="IW797_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1222820.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            8811..9053
                     /locus_tag="IW797_000012"
     CDS             8811..9053
                     /locus_tag="IW797_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAQ1222821.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            9050..9520
                     /locus_tag="IW797_000013"
     CDS             9050..9520
                     /locus_tag="IW797_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1222822.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            9648..10862
                     /locus_tag="IW797_000014"
     CDS             9648..10862
                     /locus_tag="IW797_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1222823.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(11572..11760)
                     /locus_tag="IW797_000015"
     CDS             complement(11572..11760)
                     /locus_tag="IW797_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222824.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            11851..12060
                     /locus_tag="IW797_000016"
     CDS             11851..12060
                     /locus_tag="IW797_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAQ1222825.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            12172..13785
                     /locus_tag="IW797_000017"
     CDS             12172..13785
                     /locus_tag="IW797_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAQ1222826.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            13820..15031
                     /locus_tag="IW797_000018"
                     /pseudo
     CDS             13820..15031
                     /locus_tag="IW797_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            15054..15980
                     /gene="glsA"
                     /locus_tag="IW797_000019"
     CDS             15054..15980
                     /gene="glsA"
                     /locus_tag="IW797_000019"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ1222827.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            16188..17648
                     /locus_tag="IW797_000020"
     CDS             16188..17648
                     /locus_tag="IW797_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222828.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            17869..19632
                     /locus_tag="IW797_000021"
     CDS             17869..19632
                     /locus_tag="IW797_000021"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ1222829.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            19716..20174
                     /locus_tag="IW797_000022"
     CDS             19716..20174
                     /locus_tag="IW797_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ1222830.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            20283..20966
                     /locus_tag="IW797_000023"
     CDS             20283..20966
                     /locus_tag="IW797_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1222831.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            21113..21727
                     /locus_tag="IW797_000024"
     CDS             21113..21727
                     /locus_tag="IW797_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ1222832.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            21743..21949
                     /locus_tag="IW797_000025"
     CDS             21743..21949
                     /locus_tag="IW797_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222833.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            22490..23620
                     /locus_tag="IW797_000026"
     CDS             22490..23620
                     /locus_tag="IW797_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1222834.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            23712..24467
                     /locus_tag="IW797_000027"
     CDS             23712..24467
                     /locus_tag="IW797_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1222835.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            24525..26564
                     /locus_tag="IW797_000028"
     CDS             24525..26564
                     /locus_tag="IW797_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ1222836.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(26673..27824)
                     /locus_tag="IW797_000029"
     CDS             complement(26673..27824)
                     /locus_tag="IW797_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1222837.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(27978..28508)
                     /locus_tag="IW797_000030"
     CDS             complement(27978..28508)
                     /locus_tag="IW797_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222838.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(28607..28957)
                     /locus_tag="IW797_000031"
     CDS             complement(28607..28957)
                     /locus_tag="IW797_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ1222839.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(29114..30643)
                     /gene="ahpF"
                     /locus_tag="IW797_000032"
     CDS             complement(29114..30643)
                     /gene="ahpF"
                     /locus_tag="IW797_000032"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ1222840.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(30653..31216)
                     /gene="ahpC"
                     /locus_tag="IW797_000033"
     CDS             complement(30653..31216)
                     /gene="ahpC"
                     /locus_tag="IW797_000033"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ1222841.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(31360..32022)
                     /locus_tag="IW797_000034"
     CDS             complement(31360..32022)
                     /locus_tag="IW797_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1222842.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            32206..33852
                     /locus_tag="IW797_000035"
     CDS             32206..33852
                     /locus_tag="IW797_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ1222843.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            33843..34925
                     /gene="uxuA"
                     /locus_tag="IW797_000036"
     CDS             33843..34925
                     /gene="uxuA"
                     /locus_tag="IW797_000036"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1222844.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(35033..36586)
                     /locus_tag="IW797_000037"
                     /pseudo
     CDS             complement(35033..36586)
                     /locus_tag="IW797_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            36734..37875
                     /locus_tag="IW797_000038"
                     /pseudo
     CDS             36734..37875
                     /locus_tag="IW797_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(38001..39080)
                     /locus_tag="IW797_000039"
     CDS             complement(38001..39080)
                     /locus_tag="IW797_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1222845.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(39183..39623)
                     /locus_tag="IW797_000040"
     CDS             complement(39183..39623)
                     /locus_tag="IW797_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1222846.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(39680..40090)
                     /locus_tag="IW797_000041"
     CDS             complement(39680..40090)
                     /locus_tag="IW797_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1222847.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(40270..40704)
                     /locus_tag="IW797_000042"
     CDS             complement(40270..40704)
                     /locus_tag="IW797_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1222848.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(40804..41652)
                     /locus_tag="IW797_000043"
     CDS             complement(40804..41652)
                     /locus_tag="IW797_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1222849.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            42041..43807
                     /locus_tag="IW797_000044"
     CDS             42041..43807
                     /locus_tag="IW797_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ1222850.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            43909..45168
                     /locus_tag="IW797_000045"
     CDS             43909..45168
                     /locus_tag="IW797_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1222851.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            45315..46616
                     /locus_tag="IW797_000046"
     CDS             45315..46616
                     /locus_tag="IW797_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1222852.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            46619..47470
                     /locus_tag="IW797_000047"
     CDS             46619..47470
                     /locus_tag="IW797_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1222853.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            47483..48298
                     /locus_tag="IW797_000048"
     CDS             47483..48298
                     /locus_tag="IW797_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ1222854.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            48449..49615
                     /locus_tag="IW797_000049"
     CDS             48449..49615
                     /locus_tag="IW797_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1222855.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            49845..50492
                     /locus_tag="IW797_000050"
     CDS             49845..50492
                     /locus_tag="IW797_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ1222856.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            50576..51520
                     /locus_tag="IW797_000051"
     CDS             50576..51520
                     /locus_tag="IW797_000051"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ1222857.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            51655..53106
                     /locus_tag="IW797_000052"
     CDS             51655..53106
                     /locus_tag="IW797_000052"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ1222858.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            53127..53963
                     /locus_tag="IW797_000053"
     CDS             53127..53963
                     /locus_tag="IW797_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1222859.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            53963..54622
                     /locus_tag="IW797_000054"
     CDS             53963..54622
                     /locus_tag="IW797_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1222860.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            54748..55437
                     /locus_tag="IW797_000055"
     CDS             54748..55437
                     /locus_tag="IW797_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222861.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            55443..56399
                     /locus_tag="IW797_000056"
     CDS             55443..56399
                     /locus_tag="IW797_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ1222862.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            56443..56955
                     /locus_tag="IW797_000057"
     CDS             56443..56955
                     /locus_tag="IW797_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1222863.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(57211..57759)
                     /locus_tag="IW797_000058"
     CDS             complement(57211..57759)
                     /locus_tag="IW797_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1222864.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(57848..59029)
                     /locus_tag="IW797_000059"
     CDS             complement(57848..59029)
                     /locus_tag="IW797_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ1222865.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(59265..60773)
                     /locus_tag="IW797_000060"
     CDS             complement(59265..60773)
                     /locus_tag="IW797_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ1222866.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            60932..61123
                     /locus_tag="IW797_000061"
     CDS             60932..61123
                     /locus_tag="IW797_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222867.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            61326..62243
                     /locus_tag="IW797_000062"
     CDS             61326..62243
                     /locus_tag="IW797_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ1222868.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            62236..63309
                     /locus_tag="IW797_000063"
     CDS             62236..63309
                     /locus_tag="IW797_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222869.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            63457..63975
                     /locus_tag="IW797_000064"
     CDS             63457..63975
                     /locus_tag="IW797_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222870.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            63956..64348
                     /locus_tag="IW797_000065"
     CDS             63956..64348
                     /locus_tag="IW797_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ1222871.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            64424..65359
                     /locus_tag="IW797_000066"
     CDS             64424..65359
                     /locus_tag="IW797_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ1222872.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            65492..66271
                     /locus_tag="IW797_000067"
     CDS             65492..66271
                     /locus_tag="IW797_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ1222873.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            66264..66581
                     /locus_tag="IW797_000068"
     CDS             66264..66581
                     /locus_tag="IW797_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1222874.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            66825..68435
                     /locus_tag="IW797_000069"
     CDS             66825..68435
                     /locus_tag="IW797_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1222875.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            68592..69440
                     /locus_tag="IW797_000070"
     CDS             68592..69440
                     /locus_tag="IW797_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1222876.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            69671..70150
                     /locus_tag="IW797_000071"
     CDS             69671..70150
                     /locus_tag="IW797_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222877.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            70183..72411
                     /locus_tag="IW797_000072"
     CDS             70183..72411
                     /locus_tag="IW797_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1222878.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            72540..74183
                     /locus_tag="IW797_000073"
     CDS             72540..74183
                     /locus_tag="IW797_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1222879.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(74256..74801)
                     /locus_tag="IW797_000074"
     CDS             complement(74256..74801)
                     /locus_tag="IW797_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ1222880.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(74824..75309)
                     /locus_tag="IW797_000075"
     CDS             complement(74824..75309)
                     /locus_tag="IW797_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1222881.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(75299..76111)
                     /locus_tag="IW797_000076"
     CDS             complement(75299..76111)
                     /locus_tag="IW797_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ1222882.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(76250..>77359)
                     /locus_tag="IW797_000077"
     CDS             complement(76250..>77359)
                     /locus_tag="IW797_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1222883.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        26028 a        13422 c        15201 g        22708 t
ORIGIN      
        1 cattattatc aaaataatgt taaaaataaa aaagaattgt ccatcactgt tattcaaatc
       61 attttatcaa aactacatag taaatgttaa aaagcaacat gacataatat atagaaggct
      121 ttacagtata tatgggagct tactaaaaaa gaatggagta agagttaaca caatgggcca
      181 ttagctgaag ttttaagcac ttcaaggtat gaaacagaat ggcctgtaat actacatgat
      241 tatttagact gctcaaactg ctatcagtct ggtggaatga ataaagtatt atactaaaag
      301 cagatccaac tacaacgtaa caggaaacca gttgtatcag gaaaagtgat agtacatgga
      361 gaagaaaagg ccccctttgt atgagtcagt acatatctta tttatagata aaacggtatt
      421 caatcgaaat aaaaaatctg aataatagac gtaaataact tctaaaagtc tcacacatct
      481 atcattcaga ttatttttta tttactgttt gttctacttt attttattct tctggtatag
      541 tattcgagtc atttttatag gtcaacaaat cacctggttg acactccaaa acttggcaga
      601 ttttttcgag agtggaaaaa cggatggctt ttgcttttcc attttttaga atagagatgt
      661 ttgccatcgt aatgcccact ttctcactca attcagtcac actcattttt ttttgagcta
      721 atactacatc taaattaacg ataattgcca tgtcgcacct cacactgtca aatcatgatc
      781 tgtcttaagt aaaatcgcat gttcaagcag tttttccagc acgcttgtta atgtgtatac
      841 tgcaaaagga atgaatacga cagcgattcc taccaacatc aaacctggag catcgtctaa
      901 atccgctact ttcataaatt taggaagtaa gccaaaaaag gcgaaaccaa taaacaaaat
      961 ggttcgttcg atttttttta gtatgtttaa gctttgagtg gaaaaagctt ggttatcttc
     1021 gatttttttc aatagtttca ataagaaata agcaataacg atccctagga gaagggcaaa
     1081 gtaaatgaca gaaacaaata atgtggtctg ccaatcaaaa ttgaatcctt tttcgcttgt
     1141 agcgatctga gtataaaaaa gtacgccgaa caaaataaat acggcactta tacctaaaac
     1201 gacaaatttt aatagcaatg ttttaaattt catttttatc cctcttttat ctttgatagc
     1261 taaaatatag cagaataatt attgtaaaac aactattttt tatcgaaaaa caataaaaat
     1321 atatcgttta taaaaaatga tagagtatga aggtgtggag tgcatttaac agcttttttt
     1381 gattgtaaga aacatttaaa accagttatt ttagattcta tgtgtgtctc ttctataatg
     1441 ctagtttaga tgcctgggat tatattggac aatacagttg gaggattttt tattatctag
     1501 cgaagagaag tgtaagttaa cagaactgta tgagaaaaaa gacaactacg tagtagctaa
     1561 ccgattcatt aattttttca agttcatgtt tatttacgta tcagaatttt tcttattcaa
     1621 tggtttggtg taatcaccac ttatttgtcc tttattgctt atacaatgtt atacaaaagg
     1681 aaaatcaagc attgacgcat ggggtggtac tatctagctg aaatagctta ataagcgcaa
     1741 gtagatgcat tggattagtt tataaatcaa aagtgttaga ggtattaaga agaagtagaa
     1801 aaataaaatg gattgccgaa taaaaagtac cataaatcct aaaacatgtg ccaacagata
     1861 gatggcacat gtttattttt ttcgataatt ttgtaacaaa aatacgaaga tgaaaaagaa
     1921 tagagattcc cataatgtag tagtccaact gaattcttta ctgataccaa ataccatatt
     1981 tcctagattt gatacaatca aatagccaat cgttatgatc aatgaatagt acaatctttt
     2041 catcatagga catacctcgt ttttatttat aagaatagta taaaccaaag aagtgaataa
     2101 gaaaaatgat aatataaaaa taaagtaaga acgcattaaa gtaactaaag ttatttattg
     2161 tattttttaa ttctccgaaa aaatttaata aataaataga atcttgtaat aataactgag
     2221 cataatcttt gcaattttta atcttgaata atatccttta agtatagcca gcaatcaaaa
     2281 gtaaatagag aataagtaag caattgttat ttcagtatca gactggcaat aaaacgccgt
     2341 tatgatccag caatgtttgg tagcgcttta tttactgatg ctatgctaac tgttcgctat
     2401 ctatactgtg attgtcggta gagttattta cagggtaaaa ataagtttat aggaggtgga
     2461 aaatgaataa ggggttaaaa ataatcggtg ttatcatgag tttactgcta ctttcagtat
     2521 ttctagcgat tgcgttgatc aatgcgccgt atattatgcc tgaacaattg gaacgatttc
     2581 gcttctttac tttaacgaat tactacatgc aacaatatat tttctgggca gctgtagtat
     2641 ttgccgttct tgctattatc atcttgctag tcgttctttt ctatccaaag tcaagaggaa
     2701 ctttcgttat gaaacgggaa gatggcaaac taacaatcga caaaaaagcg atcgaagggt
     2761 tggtacgttc ccatttacac gaagaagagt tcattcattc acctaaagta cggatacgtt
     2821 caacgaaaaa tcgaatccat atccatgtca acggagactt gaaacgaact tcttcattag
     2881 tagggaaaac aggtgtattg atgcaagata tcgaagaaga agtaactaaa gtactcggaa
     2941 caaaagaaac ggtcaaagtg gcagtcacat acagcggtta tgaagaacaa gaagatacaa
     3001 gagactacaa gcactctcga gtggaatagg agggccaaat aatgaaagaa ctattttcag
     3061 catacaagct ccctattatc tttgggggtc ttggactact gctagctgtt ttatggttaa
     3121 cgattggatt tttcaagaca ttgttgatca taatcatgac agcaattggc gcaggaatcg
     3181 gtttttattt gaaacagtca ggaatagttg atcaattttt taaatcatag gaggaaacaa
     3241 agatggaaaa caaaaacatg aatacaacaa atggatcagt tttggcaaat gaaaaaggag
     3301 aaaaccgcat gatgaatggt gaattgactt atgaagacaa agtcatccaa aaaatcatcg
     3361 gttatgcatt gaaagatgta gatggtctat tgacattaga tggcggattt ttctcaaata
     3421 tggctgaaaa attagtcaac acagataacg taacagcagg tatcaataca gaagtaggta
     3481 agaaacaagt agctgtggat ctagatgtga tcgtagaata cggtaaagac atcgaagata
     3541 tttacaacca aatcaaagaa ttgatcagta ctgaagtaaa caatatgaca catttagatg
     3601 ttatcgaagt gaatgtgaat gttgcagaca tcaaaacaca agaagaatac caaaaagatc
     3661 aagaaacagt acaagacaaa gtaacagaag cagcaaaatc cacaggacaa tttgcttcac
     3721 gccaaacaga taaagcaaaa tctgctgtag gcaaaggtgc acaaaaagtc aaagaaaata
     3781 acgaaccacg tgtacaataa gaagtaagaa gtcagttagg aggaatttaa aatgggattt
     3841 atttgggcat taatcgtcgg cggtatcatt ggagcaattg caggagcaat cacgaataaa
     3901 ggatcatcaa tggggatcat cgcaaacatc attgctggtt tagtaggttc agcaattgga
     3961 caagctttac taggaacttg gggaccaaca attgcaggaa tggctatcat tccatcattg
     4021 atcggtgcaa tcattttagt attagtcgtt tcattctttg cacgtaaatt ataagcaaat
     4081 tgaagaaaaa cttatgttga aatgaagaac aaatttgaga accgtagcga cactcgctgc
     4141 ggttcttttt tatatatttt atatgaagta ttctttattc cttatgaaaa aatatcttta
     4201 aaaataaaaa aagacgaaaa taaacggttt tctataaaaa gaagaccgtt tattttttgt
     4261 ctttatttgt gtgaaaaacg aaaaagagca caacacatca agcttttatt aattgtctgt
     4321 cttttataaa tgcaagtatt aattcaaaaa aacagaagaa aaaaacaact acaccttctt
     4381 ttatactcgt aatcgtagaa aggaggcgaa aaaaatgaat acattaccta tcaatacagg
     4441 agatacagca ttcatgattt tatgtacagc aatggtttgc ctgatgactc ctggtctagc
     4501 ttttttttac ggcggattag cacggaagaa taatatacta acgatcatgt ctcaatcttt
     4561 tctctccgtt gggataacaa cgattatgtg ggtatttggt ggattcggat tggcctttgg
     4621 acgggatatc ggcggtgtta ttggaaaccc ggcagatttc tttttgatga gacatgttac
     4681 aagcttcccc aacatgtttc atggtgcaac gattccattt ttaatgttct ttctttacca
     4741 actgatgttt atcgtgatta ccgttccttt gatgacagga gcttttgcag ggagattgaa
     4801 tttaaaaggc tatattatac ttgttatttt ttggaatctg ctgatttatt tccctgtttg
     4861 ccactggata tggggtggcg gatttttgga tcagatggga tttagagatt tcgctggtgg
     4921 agcagtcatc cacacgacag ctggattcgg ctctttagca tgtatcctca cacttggtca
     4981 aagaaagatt cctttaggag aaagaagtca gcatagcagt cttatggcag ctgctatcgg
     5041 tacaggactg ctttggtttg gctggttcgg ttttaattcc ggtggtgcac ttcgagcaga
     5101 tgaacaagca gtcaatgcat ttggttcaac attcatcgct cttgcatttg cgatgattac
     5161 ttggctgatc attgcaaaag taaaaggaaa tggctttgat tttgtcgacg tactaacagg
     5221 atccgttgct ggattggcag ccattacgcc ttgtgccggt tatgtagaag caaaaagtgc
     5281 catgctgatt gggatcattg cgggtatcgt ctgtcatgct gctgttgatt ttcgtaaaaa
     5341 gaaacaatgg gatgatgcac ttgatgtgtg gggtgttcat ggcatgggcg gattcacagg
     5401 tacgattctg atagggatct ttgcatctaa cagccattta ctaacgaatc caagcagctg
     5461 gtacttctta gggattcaag tgattggtgt catcattact gccgtgtatg cctatgtgct
     5521 gacaacagtg attttaaaat cggcgatgca ctttacaacg atcactacta caaaagaaga
     5581 acaagaagaa ggattggact ttttcttctt tggcaacaaa ctcaattgaa acaaattcaa
     5641 atagaaaaga ggaagattca tgcaacaaag caaaacgaaa attcctgcaa aaaaattaac
     5701 gttctttggc tttttggcta tgaccatctc aatggtcgtt tcattatatg aatatcccac
     5761 attcgcgacc tcaggcttca gcttagtatt tttcttgtta ttaggcggtt tgctatggtt
     5821 tatccctgtg gcattgtgcg cagcagagat ggcaacggtg aaaggatggg aaaaaggtgt
     5881 ttcccaaaca aaaagatatt aagaaacagc ccatgtttac ctgcttgcaa acaaaagaat
     5941 attgctacta gactaagaga tactacataa gtgtgttggt atctcttttt tgttgccgtg
     6001 aaacagcaga ctatttcatt tctgcttgat taccttaaaa cttattagtc tatggtttgt
     6061 caagggcttg ttgcgtgaga aatgctgaat atcacccttt acaaaacata ggctgataag
     6121 taacttttca aaagcagaaa agaaatggca atgcttataa caatatgata taatgcagat
     6181 gaagacaaaa aaggaatttg tcgagctggt tagttcgaga taattttgag tttgttaaga
     6241 tgtggtgcac ttgatggaat cacaggtacg acatcatatt atggaggatg aaatagatga
     6301 taggtttgat tgttgcgagg tcaaagaata atgttatagg caagaatggt aatataccat
     6361 ggaaaataaa gggagaacaa aagcaattta gagagttaac aacgggtaat gtggttatta
     6421 tggggcgaaa gtcttatgaa gaaatcggtc atccgttgcc taatagaatg aatattgttg
     6481 tttccaccac aacagagtat caaggagata atttagtttc agttaaatca ttagaagatg
     6541 cattattatt ggctaaagga cgagatgtat acatatctgg tggatatgga ctatttaagg
     6601 aagctttgca aatagtagat aaaatgtata tcacagaagt agatttaaat attgaagatg
     6661 gagatacatt ctttccagaa tttgatatca atgattttga agttttgata ggggaaacac
     6721 ttggtgagga agtgaaatat acgagaacat tttatgtaag gaaaaaatga attgagtaaa
     6781 ttttggattt agggtgtttt cataaatata ttgcttttta tgcatatata aattgttgta
     6841 agacaaattc cagtttgtat ggggcgataa aatcagaatt tgagagagga ttgaatatga
     6901 ataaggaatg gtctgaactt aataaaacaa tgcaggcaca aataaaaaag aaagatactt
     6961 ataagagggg tattgatact ttacttactt tacgaagtca gttaatacaa accttagtat
     7021 ctttcaaaga agaattatgc agagaagatt ttaactcaat ccccttcata aacgctgatg
     7081 gttatcatag taagacgatt gcttattcta tttggcatat tttccgcatt gaagatatag
     7141 ttgtgcatac agtgataaat gaagatgaac aagtattttt tgcaggcaat tatcaagagc
     7201 gtatcaattc acctatcatt acaacaggaa atgaactcat gaaacagcag attgcagact
     7261 tttcaaaaca gcttaatctg gaagaattat atttatatat tttcgaagta tgggaaagca
     7321 ctgagaaaat gcttgaacgg ttatcttatg atgaattgaa aagaaaaata ccgaaagaaa
     7381 gaaaaggata cttagaatcg ttgaatgtag taaacgacaa tgaaaaagca atttggctga
     7441 ttgattattg gtgtaataaa gatatttgtg gactaattca aatgccgttt tcaagacatt
     7501 ggattatgca cacagaagcc tgtttgcgta taaagaacaa gatacattca taggcaaaaa
     7561 tttcagtttg tagaactaag gaaattggaa tttaaggagg aataaagtat ggttagtgat
     7621 tttataagag cagcatttcc atggatttta atgggtttat ttgtagcaat cagttgttcc
     7681 cttatgagta aaaaagagaa ataaattcct gtttgtattt ataaaagaga aaaaatgaga
     7741 aaagtagttt tatttattgc catgagcctt gacggatata ttgcggacgg taatggtgga
     7801 gtagcttggc taaatggtca tggaaatgac aatgaaaaca tcgacactta tacagaattt
     7861 accaaagata tagatactgt cttaatgggg tggaatactt ctcatcaggt tgttactgaa
     7921 ctttctccgc aagaatgggt atataataaa tttacaactt atgtattaac acataaagag
     7981 tgcaattctt cccaacaaat tcattttaca agtgaaaatc ctgttttatt attggaacgg
     8041 ctaaaacaag aagcaggaaa ggatatttgg atttgtggcg gagcaaatct tgttcagcag
     8101 ttggtaagca aaaatatgat tgataaatat tacatttctg ttattcctac tctgttagga
     8161 aatggggttc gtcttttggg caacatagac agggaaataa agttaaggct ttgtaaaaca
     8221 cagacttata acggaataac cgatttgatt tatatgcgta gataactaga gagaaacaga
     8281 aaagctgtta aaccgacgga gcgatccgac cacgttggcg ggaaaatcct taagattttt
     8341 ccgccatgct tgcaaggggg cggttcgctt cggcggttcg gcgagcttgc gagccgttca
     8401 aagcccccgt tatctaacaa ggggggcaca aaaaacaaga aacactatac actaacaagc
     8461 ggaaaacgct gtatttataa ggcaaaaccc catttttaca gtagtgatat aaattgtacc
     8521 tatatcacta tgaaaattga atagtaaata tgagaaagac agttgatttc aaaaaataag
     8581 aaattgattg tcgttttctt ttgcagaaat ttaagtgcag gcaattatgg tagttgtcgt
     8641 aattgctgtc agctcagtgt ttatcactga tggtgtgaaa gatttacggt tggagatgtt
     8701 caataagatt attggtgctt aattattttt attgtagtgc tgatatgaga atgttataat
     8761 tggggtataa aacatacaaa taaatttgct gaaaaatgag gtatcaaaat atggaaaatg
     8821 aattactgga aaacttagga agacttcaca caactgaact tggcgttatt agaattaaaa
     8881 agaacttatc tttgaatgta gaaaatgtaa ttgaatggtg caaagaaaaa attagccttt
     8941 ctaatgcgga aatcacaaga aaaggaaaaa actggtatat aacgattgat aattgtatta
     9001 taacggtaaa tgcgtacagt tataccgtta tcactgctca caaggtaaaa tgataaattt
     9061 ttctataaag gaattaaacg catttgcagt tattgggcag gaagtagaac tgactaatta
     9121 tcaaaaaaag aatattcaga ttagtacaca gttttggaga aagtttaaca gtagtttgaa
     9181 aaaagcgtat ctttcacaat caggaaattg ggtaaaatat gcttttatgg aaagaagaaa
     9241 tggaaaactt tattatttct gttctattcc ccaaagaacc attaccccag agggctttct
     9301 gtataaagaa ataccgtctt ataaatattt ggttgtggag catattggtg ctatggataa
     9361 aatatatgaa acttatggca agatttatga agaaattatt cctagtacat cgtatattcc
     9421 tataaaagat attatcctac actttgaaaa atacgattat cgttttcatt ggaatagtga
     9481 taattcagtt attgaaattt ggatacctat tcaagattag aagcaatttt atatccatac
     9541 acacaaagca gttagtctta ggattgactg ctttttctat acagattttt aatgacaggc
     9601 aattatcaat tatggtagtt gccttttttg attggaggaa ttgaagaatg aatgataaaa
     9661 actttattga agaactaaga caaaagcgtg aggaatacgg agtaacacaa acaaggcttg
     9721 ctgttgcctg tggtatcagc cgtgaatatt acaaccgcat tgaaaaagga aaacagccat
     9781 tgaatgacga attaagagaa atcatagaaa aacagattga gcgtttcaat ccgcaagaac
     9841 cactattctt attgattgat tactttcgtg tccgctttcc tactacggac gcattggcga
     9901 ttatccgtga tgtattacaa ctgaaatcta attacatgct ttatgaagat tatggaaaat
     9961 acggatatga aagcaaatat gtacttggcg acatcaatat catgtgttcc atgcaggagc
    10021 atttaggtgt tttattggaa ctgaaaggca aaggctgtcg gcaaatggaa tgttatctgc
    10081 tggcacagga acgctcatgg tatgacttca tgctggattg tatgacggct ggtggtgtga
    10141 tgaaacggct tgatttggct atcaatgata aagcaggaat attggatatt ccaaagttaa
    10201 aggaaaaata caaggctggc gaatgtatgt cctacttccg tatgcagaaa gattacagcg
    10261 gtacagaaaa atgcggtagc gatttaccaa agaatacagg agaaacctta tatctcggtt
    10321 caacaagtag cgaattatat atgtgtgctt atcagaaaaa ttatgagcag tatgtcaaga
    10381 atggcacaga aattgaagat acggagatta agaaccgttt tgaaatacgc atgaagaacg
    10441 aacgagccta ttatgcggtt gtagatttac tgacctatcg ggacgctgaa cgcaccgctt
    10501 tttctatcat caatcattat gtccgctttg ttgacagaga ggacgacaag ccaaaaagtc
    10561 aatggataac aaatgatgat tgggcatggt ttgtagggga aaacagagag ccgatacgct
    10621 taaccacaaa gccagagcct tacactttac aaaaagcgtt gcattggcta caaagacaag
    10681 tcgcaccaac cataaaaatg gtacaggcat tagacagaga aaatcgtaca acgatactga
    10741 aagatatgat tgagcaggca gaactaaaag acaagcataa acatttatta caattagaga
    10801 aatcaaccat agaagaacgt atagataccg ctgttccaca agagaatgac ggtatttttt
    10861 aatgtaattg aaaaatttca gatttagtca gcaaagagct gttttcattc cctatgtgaa
    10921 gtgaaagaag aaaaataaat tttttattaa atctgcaaca aaatggctac ttgcgtacag
    10981 atatggtgcg agaaaaggaa atctaaactt tttttgaaaa taggtcatta aatcggggct
    11041 tgctgtccct atatgaagtg aaagaagaaa aaagttttga aaaaatgacg gaaattcgtg
    11101 attagtggac agtcttctgt gaaaagagga aaaacatcat cattttgtat tcaaacaggc
    11161 attagcagtt gagtgttgat gtgaaaagat gaaaatactt tgttctcaac ttaacaattt
    11221 aaggtttccg ttccctatat ggtgcaaaga aaatatttca ttctattgtt ggcagtaacg
    11281 gtatggtttg ggtagttagg gtgtgaaaag gaaaatattc ttttttcatc atcaacttag
    11341 caaaatgaca ttttctatcc ctatatggtg catgaaaaga agttaaaatt tttccattta
    11401 aacttagcaa atggacgttt gctgtacaga tagtagtgaa aaaggagaaa atcaaccgtt
    11461 gattgccaaa atgaaccgtt ccatttgtac taataatgga aaaagttttg gattttggtt
    11521 acgtttctcc tcttttccaa cgcgatatat aggaaagacg atttttttct ttcaatcgtt
    11581 ctttgacaac tgaataaagc tattcaatac gtttgacaga cagctagccg ttgaaacaaa
    11641 tacgccacga cctttcccct gcaagagcga gcgaaaacct atttgtgttc ttgtaaaaga
    11701 tttgtcctct tttatcgcca tgacctgtac tgtcagataa tgatactccc gtacagccac
    11761 agcttgagca ttaagagcgt cgcacgcaat gggtacggct acataagaac tatgcagagg
    11821 tggaactcct gtgggctatg acaaaagccg ttgaattgct tagaaagatt ttacagaaag
    11881 ggggtatgtg ttattgataa tgctgtaaaa acaattcaaa agaaaaatgg caagtgtggc
    11941 attggcaaca gaggaaagac aaagattgta gtaaaagagc atttttccga aaagggcaaa
    12001 acaatggaag aacttctaac tgatgtcatg ctggaaaaag caaagcagac aatcgcataa
    12061 aatcggtgca gttgtaagaa atagattgaa tgacaaaaag tacccgtgtt atactttact
    12121 tgcaagcagg tattgtaaac acgggttagt tataaaggag gataactgac aatgaaacaa
    12181 cagatttaca atactgcact ttatcttagg ttaagccgag atgatgaatt acagggcgaa
    12241 agttccagca ttaccacaca gagaagtatg ttgcgtttat atgcaaaaga acatcatttg
    12301 aatgtcattg atgaatatat tgatgacggc tggtcgggaa caaattttga cagaccgagt
    12361 tttcaaagaa tgattgagga tatagaggca ggaaaaatca actgtgttgt aacgaaagac
    12421 ctttcccgtc ttggcagaaa ttatattatg acaggacaat atacagaatt gtatttccct
    12481 agtcataatg tccgctacat agcgattgat gacggtgtag acagcgaaaa aggcgaaagt
    12541 gagattgcac catttaagaa catcatcaat gaatgggtgg caagagatac aagccgtaaa
    12601 gtcaaatcag catttaagac aaagtttgcg gagggggctc attacggtgc ttatgctccg
    12661 ttaggatata agaaacaccc tgacatcaaa ggaaaactgt tggttgatga tgaaacaaaa
    12721 tggattattg aaaaaatctt ttccctagcc tatcaaggtt acggaagtgc aaaaatcaca
    12781 aagcagttaa gagcagaaaa agttccgaca gcgtcatggc taaattttac aagatacggt
    12841 acttttgcac atatctttga gggaaaaccc gaaagtaagc gttatgagtg gacgattgct
    12901 catgtcaagg cgatattgaa aagtgaggtt tatatcggaa acagtgttca caatatgcag
    12961 tctacggtat cgttcaagag caaaaagaaa gtgcgtaagc ccgaaagtga atggtttcga
    13021 gtagaaaaca ctcacgagcc gattattgac aaggaagtgt tctatcgtgt gcaggagcag
    13081 ataaaatcaa gacgcagaca gacaaaggaa aaggcaacgc cgatttttgc agggcttgtc
    13141 aagtgtgcgg attgtggttg gtctatgaga tttggaacga ataagacaaa taaaacgcct
    13201 tacagttatt atgcttgcag ttactacggg cagtttggca aaggtaattg ttctatgcac
    13261 tacattcgtt atgatgtgct gtatcaagcc gtattggaac gcttgcagta ttgggctaag
    13321 gcagtacagc aggacgaaga aaaggtattg aacaagatac agaaagctgg caatgcagag
    13381 agaatacggg aaaagaagaa aaaggcaagt acattgaaga aagccgagaa cagacaaaat
    13441 gagattgacc gtttatttgc gaaaatgtat gaagatagag cctgtgagaa gataacagag
    13501 cgaaattttg tgatgttgtc cagcaagtac caaaaagaac agatagaact ggaacagcag
    13561 ataacaagcc taagagaaga actaagtaaa atggaacagg atatgatagg tgctgaaaag
    13621 tggattgagt taatcaagga atattccgta ccaaaggaac tgacagcacc attattaaat
    13681 gccatgatag aaaaaatcct catacatgaa gcaacaacga atgaggataa cgaaagaata
    13741 caggaaattg agatatatta ccgatttatt ggaagagttg agtaatcaac aagagtaata
    13801 tttttaacta agggaaaccg gcggtgtcta tacttgggtc agtcgcacat tgggaaaacg
    13861 attcggtttt gccgcaatct ttttccaatg gtttgagata acagttggct acttgaccat
    13921 gctgtatttc ttaactggtg cattatccta tgcaacgggt attggtgcta tacaaaacaa
    13981 taaatatctg aaattggcaa tcctattgat tattttctgg gctattttac tctcacagtt
    14041 aaaaggaacc aaatacactt cattgattgc tcggatcggt tttgtagctg gtatcttgtt
    14101 gccagcatta gtcttgtttg gcttagggat ccattatgtc gcttcaggcg ctccactaca
    14161 aacgatactg tctatgaaaa cattgattcc tgatttttcg aaattgccta cgctggttgt
    14221 ttttgtttcc tttattttag catatatggg cgtagaaaca tctgccagtc atgcaaatga
    14281 aatggaaaac cctaaaaagg attatccatt agcaatgttt gtattggtta ttctggcaat
    14341 cattttagat acttttggcg gattgaccgt cgctactaca gtacctcaac atggtttgtc
    14401 tcttaataca ggggtgattc aatcgttaga attcctatta agacacattg atccaaatct
    14461 tgcatggatt gcgaaaattc ttgcgattct tgtttgttta ggtgtcatcg gagaaattgc
    14521 ttcttgggta acctctcctt caaaagcact tcacgtagca gcagaagaag gattacttcc
    14581 tgaatatttt gcaaaagaaa acacacacgg tgttcctgct catttgatga tcgcaaatgg
    14641 gatcgttgca acaatctggg cagccgtctt tacattaagc ggcgggggga ataatctttc
    14701 atttcttgca gcgatgtcat tgactgttgt aatctacctg atgttgtact ttatgttttt
    14761 cgcgggctat ttgaagctgg tttttaaaga tgacagtctt gaaagagcct atcaggttcc
    14821 aggcggaaaa gttgtaaaag taatcgttgc ttgtttaggt cttttgactt cactttttgc
    14881 tttttgtatc tcatttgttc cacctgctag tatcgcagct ggacagcatg ggatttatga
    14941 aacgattctg attgtttctt atcttatcgt cttagttatt ccatttacga tctacgcatg
    15001 ccgtcataaa ttccagcatg cagaaaaata aaaaatgaaa gctggttatt caaatggaag
    15061 taaaagaatt agaacaattg attggaaaaa atttccacta ttatcaaaca ggacaagtgg
    15121 cggattacat tccagcatta gctgaagtgg atcccaaaca attaggtatg gcgatttatg
    15181 atttggatac aaaacaactg attgagggag gagacgccga tgtgcgcttt gccatcgaaa
    15241 gcatgtcaaa agttcccgta ttgttattag caatcaaaga caacggaata gaaaaagtct
    15301 ttcaaacaat caacacggaa ccaaccggtt ttgccttcaa ttctatcatg aatatggaaa
    15361 tcaatcatcg caaacatcca atgaatcctt ttgttaatgc aggagcgatt gcaacaacct
    15421 cgttgattgc cggtaaagat gcagatgaaa aatttgagcg aatcttgtca tttatgaaag
    15481 agatctgtga tgatcctaat atcacattga atgaagaaat ctatcattct gaatcaagaa
    15541 caggtgatat caatcgctct cttgcttact atatgaaagg taatcaaatg atcgaaggag
    15601 acgtaccgga gatactcgat gcttatttca gacaatgctc cctcaatgtc accgctacag
    15661 gcatagcaaa attagctgct gtgctggcaa ataaaggaat tgctccttgg aatggcaaac
    15721 aaatcattac tgaagaaagt gctacaatcg tcaaatcaat tatgacaact gctggtttgt
    15781 atgatgaatc aggagagttt tccgtccatg ttggtgtacc tgcaaaaagc ggtgtaggcg
    15841 gcgggttgat ggcagctgtg ccaaatcgtt acggcatagg tgtcttcagc ccggcactag
    15901 atccatttgg taacagcgca gctggtatcc aattgttgaa agatgtagtc aaagaactag
    15961 acgctgacat ctttgaataa ccattcttga aagaggctgg ggtaaaagtc caaacggctc
    16021 aaagctacct acaatgaggc tgaaattgtg agcttgtttg acttttgtcc cagtttcttt
    16081 ttttattggt ttttgaaagg gcttttctaa ataaaaagga aacaaaaata cgatttacag
    16141 atatgattaa ataaaatcat aagaaaaact ggaggcgtgt atcaggaatg ctggaaaatt
    16201 acatcgagcg aaatatattt agaaaagtgt atctgtgtga acaattattt gaattccaag
    16261 aaatagacat cgaacaaaca gctatttctc taagggtgac tacaccaacg atccttcacg
    16321 atctagaaag tctagccgag tgtctagaat attgtataaa agaacaagta agagagaagc
    16381 ataagtataa actcgtattc aaacatggta ttgctctttc tgagctcaca cagtttctgt
    16441 acggtcaatc ttatttttta aagtttttaa gttattattt taatggtata ttcacgagta
    16501 cagaactggc tgatcttgaa tttatttctt tgagtaaagt atatacaatc aaaaaaatcg
    16561 tattggattt ttttaaagaa aatgattatt tgaaaaataa agaaatcatt atcccagaat
    16621 ttgactcgcg gaacatcttg ctagctttag tacgttatgt caattgggaa ggctatgaaa
    16681 ataaaaatcc gtatgtagaa gaagcttgta acgagttgat tgattatgta gagactcatt
    16741 tttttaaaag aagatattcg gaagatgaaa agtcctttat catacgagaa atagagatcg
    16801 caatcggaag aaaggcattt ccccttggac tgaaagaaga agataagaaa agcgcagaaa
    16861 cgaagccgtt atttcaatta gtctgtcaag gattggaaga gcaaaaagga attctagact
    16921 tacaagaaga agatgtctat tatatttttt ctttgttcaa cagtagaaat tataccaatg
    16981 agaacatgga gctgttaaga aaagatttcg aagttgttta taaaaatttt gttctcaatg
    17041 acctatcact gaatgaattg attttacaga ttattagtat cgctgaaatc gacgatgcag
    17101 aaaattttat tttcaagcaa tcctttttac aatttgtccg tacgttgtgg gcagatggac
    17161 aagtattttt accggaaagg atctatcttc tatcagaaaa agaacaactt ctgtacggaa
    17221 aaattgtaga aatattggaa gagtggaaaa agaaaaatca aatccaacta agatggaatg
    17281 aaaatttcat tcgaaaattc gtcaaaagtc tttctttgat caacagtgaa caagcgggga
    17341 gacaggaaat agaaattttt gttgtttcag aaagttcagt caaacaattt ttttatcgaa
    17401 cccaatttca tttgtatgcg gaagaaggca tcgctgaaat aaatcctatt ttatttcgtt
    17461 cattagaaga attgcctgat gaatgtctat gtgcgaaaaa aagagtggtt ttgtgcgatg
    17521 ctgctagcta tcaaaatgga ttagaaactg aaaagacaaa gatctatcca atctcattaa
    17581 aaggtgcaaa catccatttg tatcatattg ctcaggaact tcacctatta aaaaaacata
    17641 tttcttgatg agaccttaat taggagacct tctacaaagc tttttaattg tctgtagaag
    17701 gtttttattt gagtcctgat tcgtgcgaag atctacttat ataacaagct ttctctgaaa
    17761 tttttttaaa atgctattga cttagagtca actctaagta gtagaataga taaagtgtaa
    17821 caaatctatt ttcattaaaa gaagatagta cggaggagag aatgtattat gagtacagta
    17881 ttacatgtat tggatgcaat caatagcccc gcagatgtca aaaaattatc tgttgaggaa
    17941 atgaaacagt tggcagaaga gatgcgccaa aaaatcttgc aaaaggacag cgatgtaggt
    18001 gggcacgttg gtccgaatct aggtgtagta gaattaacga ttgcgtttca ttatgtattt
    18061 gattcaccaa aggataaaat cgtttgggat gtttcccatc agtcttatgg tcataagctt
    18121 ttaactggtc gaaaaaatgg ttttgaaaaa ggacattacc gtgaagtgag cggatattcc
    18181 aaccaacatg aaagtaaaca tgactatttt acagtggggc acacatctac atctatcagc
    18241 ttagcaacag gtttagctaa agcgagagat ctgaagaaag aatcaggaaa tgtggtagca
    18301 tttatcggtg acggctccct aagtggtggc ttagcttttg aaggattgaa caatggtggt
    18361 gttttacaat ccaatctaat cgtattagta aacgataatg agatgtctat tgatgagaat
    18421 catggcagtt tatataaaaa cttagctgag cttcgtcaaa ccaacggtaa aagcagcaat
    18481 aacctattta aagcaatggg atttgattat cgttacgtgg ataatggaaa cgatctggaa
    18541 acggtgatcc gtttatttga agaagtaaaa gatattgatc atccgattgt acttcacgtc
    18601 catacggaaa aaggtcgcgg ttatcagccg gcaatcgata ataaaatgaa atatcattgg
    18661 catgtaccat ttgatctgtc caccggggag gcaaagcaag taacttctgg agaaacattt
    18721 aatatgatca tcatggatta tttggataag aaaatgcaaa caagtacagc tcctattgcc
    18781 gcagtcaatg cagcgatacc aggcgccttt ggactgaaaa cattccaaga aaaacatcca
    18841 gatcattatt ttgacgtggg gatcgctgaa cagcactcta tcactttcgc ttctgctttg
    18901 gcttctcagg gagtacgtcc agtcgtcttc caatcaagta ctttcttgca acgggcatat
    18961 gatcaattat cacatgattt agcgatcaac gagaatccag tgattatttt agtcggtggc
    19021 ggaacgatct cagctagtga ccgaacacac caaggaacat ttgacattcc ttacttatca
    19081 agtatcccga acatcaaata tcttgctcca gcaacaaaag aggatatgat ctctatgcta
    19141 gattgggcgt tcgaacaaac agcgcagcct gtagcaatcc gaattcctac acatggtgta
    19201 gaacatggta cagctatcgc aaatgattat agtattccag cctatcaaac agtaaataat
    19261 ggaggaaaag tagcaatttt agctttaggc ggttttcttg aattaggaaa agaaacagta
    19321 aaagctttta gaaaaaaagg aatcgaagca acgttgatca atccaggaac gatcacagaa
    19381 aatgacgtag aaacattaga taaattaaaa gaaacacacg aattagtcat cacattagaa
    19441 gatggtagtt tgtcaggagg atttggcgaa aatatcagtc gttattatgg tgattcttca
    19501 atgaacgtat taaattttgg tgcagagaaa gaattcacgg atagcgttcc tgtagggcag
    19561 ctttatgaac gttatcattt aacaccaaaa caaatcgttg aagatagtct gcgtgtcttg
    19621 aatattcaat aattcattat gctcaccatc cagataattg tttatactag tatctggatg
    19681 gttttttcat tataaaaagc aaaggggtgg atgttatgta tcagcaagta gaagaatatg
    19741 tcaaaaagtt ccaagaaaaa tatcctagtt atcaatcaga aaaattaggt ttcttttcat
    19801 ttggggatac agaaaaaatg gcagacgagt tgagcgaact agttgtaaaa aagataaaga
    19861 ctggtacatc aagtggttac gatctgtata aacaagagaa tgagccttta ccaaaagttg
    19921 gtcaattaga cgtggtcctg aatggaaaaa atgaaccagt ttgtatcatc aaaaatataa
    19981 atgtgaccat cttgcctttt agtgaagtca cgaaagagca tgcttggaaa gaaggagaag
    20041 gggatcggac attagcttat tggcggaaag tccatgctga gttttttaaa catgcttata
    20101 aagaagcgtt gaacatagaa tttacagaag agaagctggt cgtatacgaa gaatttgaag
    20161 taattttctt ttagtaagaa aggtctgctg aaagaaatag ctcattattg tctcctgaat
    20221 agaaaagggt tacattttta tatgaagctg tatttttata taacattact ggaggagata
    20281 ttgtggatac actggaacaa atcaaaggcg gagtcgtcgt ttcttgtcaa gctttggaaa
    20341 acgagccgct acacagttct tttattatgg ggagaatggc tttggctgca aaagaaggcg
    20401 gagcagttgg tattcgagca aatacgacag aagatatcaa tgaaatcaag cgaatcgttg
    20461 atttaccggt aatagggatt atcaaaagag attacgatgg gtcagaagtg tttattactg
    20521 cgacagaaaa agaagtggaa gaacttttgg agacgcaagc agaaatcatt gcactggatg
    20581 caacttctcg taaacgtccg acagaaaaaa caactgctga acttgtccaa atgattcacg
    20641 aacacggtcg gttagctatg gcagatattt cgacttttga ggaagcgctt caagcgcaag
    20701 aagatggttt tgatcttttg tctacgactt tagcaggcta tacagaatat tcacgaaaga
    20761 cgcagacacc tgattttgat ttattgaatc aaatcataga acaagtcgat atcccagtaa
    20821 ttatggaagg acatactgac tctcctgaac aagtagagaa agcatatgaa ttaggtgctt
    20881 tttcagtcgt agttggatct attatcaccc gtccgcaatt gatcactaaa agatatgtcg
    20941 aagccgcaag taaagcaaat aactaaaaaa cacgcacggc acagattctt gacaaaaaag
    21001 aaaaattgat agatagttta aagcagattc taagaagatg aaaaaagcat tacttttcat
    21061 tttgtcttca agatctgctt tttaacgaat tttatagaaa gaaggcgaaa ccatgtttga
    21121 acggagtcct tcagaattga cagaacgtga aaattacaag ttgctgattg gttcagtcat
    21181 ccctagacca gtagcagtag taacgactaa atcagcaaaa gatatagtga atatcgcacc
    21241 cttcagttac ttcaatatcg tcagttcgaa tcgacccatc ttatccttgg cgatccagag
    21301 aaaacaagat gcagtaaaag acacagcaaa gaatattctt gagacaagag aagctgttat
    21361 ccatatcctt gatgaggaca acgtagaaga agtaaataaa acagcagcca atctgcctaa
    21421 tgaggaaagc gaactgtacc tgactgactt gactttaact gattcagtaa tcatttcagt
    21481 accagcattg acagaagcaa aagttcgttt tgaaacatcc ttatatgaac atgtcgagat
    21541 acggaataaa aataaagtaa cagctgattt atttttgcta gaagtacaga aatatcattt
    21601 aaatgaaaca gtctatgatg ataagacagg aaggatcgat ccaagaaaat tgaatgctgt
    21661 aagccgtttg gcaggaaatg attatgcagc aataggagaa attttcacga ttgcacgtcc
    21721 cgaataaaaa ggagcactag ttatgttaaa aataagctat aaaccatcga cagattcaaa
    21781 agaaatgaaa aaagaatatg aaacagtgaa tgattttctg caagggcagt acttagaagt
    21841 tccgccattg caagaccatt ttgtcgttac tactgttact ttagatggta aggaaataga
    21901 aatgccagat caaacgatta gcggattgtt taattacttt aacaaataaa tatttattcc
    21961 gaaaagtgaa aaaacatata aaaaaacaca taaaactaaa aaattatcaa agaatcctgg
    22021 tgcaactgaa agaatttgca acgggattct ttcaacttat ttgttatttt tttctaaaaa
    22081 atattaaaga ttgttaaatt tcactatttc atttcaaatt ccattgccta atcattagga
    22141 aaagtctatc atgaactcgt aaatagaata tagtaataac cttgttaggt gaggctccta
    22201 tacaaacata ggctgttgcc cagaaacgtc gaaagtcgcc aatgggtaaa acagaaactg
    22261 ttggattaag gctttttcca atagctaaga atgttttctt tacgttgtat agtgctaaaa
    22321 ctcggacgat gagatgatgc taggtcagaa ttcttgttcg aaaaggtttc tgaccttttc
    22381 tttatatttt tgtgactgac ttatttgatg agacaaactc gctcctcacc tgacaagtgg
    22441 attgctgtgt gaggaggaga tgcggtgaaa aaagatcgat tggtggattt tgggtcctta
    22501 tttgactttg tccatgatcg gtcttttaga agtatacagt gccagttctt atcgtttatt
    22561 gcaagccgat gaaaatacaa aaagtctatt attgcgtcaa ctaattttca tatttttgag
    22621 ttggagcgtg atcttcttag ctcgttcagt caaactacac tatttacttc accctaaaat
    22681 agcaggatac ggtttagcct tatcgatttt ctttttagta ttagtaagaa tagggatatt
    22741 cggtgtcact gtcaacggcg cacaacgttg gatctctctg tttggcattc aattccagcc
    22801 ttctgaactg gcaaatcttt ttttgatttt ttatttaagc tggttttttc gtgacggaaa
    22861 tagtagccca aaagatctaa aaaaaccatt cctgattaca gtaggtataa cttttctgat
    22921 tttatttcag ccaaagattg ctggagcatt gatgatcctt tcgattgcgt gggtcatatt
    22981 ttgggcagcg gcggttccat ttaaaaaagg gatctatcta atcgttactt tttctgcatt
    23041 gctgattgga gcagcaggcg gggtattata tttaggaaat aaaggttggc ttccacagat
    23101 gtttaatcat gcttatgaaa ggatcgcgac tttaagagat tcttttatcg atagtcatgg
    23161 tgcgggctat caaatgaccc attcgtttta tgcactatat aatggtggca tatttgggcg
    23221 gggattagga aatagtatca caaaaaaagg gtatcttcca gagacagaaa cagattttat
    23281 tttttcgatt attacagaag agcttgggct aatcggtgca ttgtgtgtgc ttttcttatt
    23341 gttctcatta tgcatgagaa tcttttgttt aagcagtcgc tgtaaaaatc agcaagctgg
    23401 attgtttctt ttaggattcg gaactttgct attcgttcag acgataatga acgtgggaag
    23461 tatcgcgggg ctgatgccaa tgactggtgt gccattgcca tttgttagct acggaggaac
    23521 aagttatctg atcctttctt taggaatcgg aatcacgttg aatatttctt caaagataca
    23581 ggccgaagag ttggcgctct atcgacctga aaaacaataa agagaaactg agaaactctc
    23641 agtttgaatt atcataaagt acaatctttt atttttcaag cgagaggttc ttttagaaag
    23701 aggaattcgc aatgaaagta tctcaaaaaa tcatattatc tattttgtta ctgcttacat
    23761 tagtagttgg ctatttttcg attgagttta tccatggatt ttcgtctgca aaacagagtt
    23821 caactgtgaa aaaggtagag ccgaaaagta tccctaccac actaaatgtg gctttgattg
    23881 gttcagatgc tcgttcgaaa gacgaaaacg gtcgctcgga ttcacttctg gttgcacaat
    23941 acaaccagaa aacacaacaa gcaaaactga tctctatcat gagagattca tatgtcgata
    24001 ttccaggtta cggaatgaat aagataaatg cagcttactc ttatggggga gttgatttat
    24061 taaatcaaac attaaaggaa aatttcaaat ttgaagctcc ctattatgca agtatcacat
    24121 ttcaagattt tatcgattgc gttaatgaac tgtttcctgg tggagtcaag atcgatgcag
    24181 aaaaatctct agatttagat ggcgtatata taaacaaagg agagcaagtg atggatggta
    24241 atactttact ccagtatgct cgatttcggg aagacaaaga aggagacttc gggaggatca
    24301 gaaggcagca gcaagtgatc aaagctatct ctcagcagct gaaagacgta acttcaatat
    24361 tcaagctacc caaagcggtt gggaaattac ttggtagtat ccaaacgaac cttccagaaa
    24421 gtgtgcttct ggattgtggt atggatttcc tcaaagacat atgttagaat aaacaggtat
    24481 aaatagtgaa ataaaggaat gacaagcaag agaaggagga aaaaatgaaa agaagtgaca
    24541 agcacggcaa aaatcgaaca ggcgcttata ttgccggcgc agtgatctta atagcagctg
    24601 cgggtggcgg ttatttttac taccagcact atcaagaaac ccaagcagta gaagctggag
    24661 aaaagacagt tgagcaattt gtccaagctt tgaacaaggg agattataac aaagctgcag
    24721 aaatgacatc aaaaaaggcg gcaaataaaa gtgcattatc tgaaaaagag atcttagata
    24781 aataccagaa tatatacggt gctgctgatg tcaaaggact tcagatatcg aatctaaaag
    24841 tagataaaaa agacgattct acttacagtt tttcatataa agcaaagatg aatacctcat
    24901 taggtgaatt gaaagatctt tcttataaag gaacattaga cagaaatgat ggtcaaacca
    24961 cgatcaactg gcaacccaac ttggtttttc cagaaatgga aggaaatgac aaagtaagcc
    25021 tgaccacaca agaagcagca agagggaata ttatagatcg aaatggtgaa ccattagcaa
    25081 caacaggcaa actaaaacaa ttaggagtcg ttccaagtaa acttggagat ggaggcgaaa
    25141 aaacagccaa tatcaaagcg attgcttcct ccttcgactt aacagaagat gctatcaatc
    25201 aggcgatttc acaaagctgg gtacaacccg attactttgt cccattgaaa atcattgatg
    25261 gagcaacgcc agaacttcca gctggagcta ccattcaaga agtagacggc agatattatc
    25321 ctttgggtga agcagctgct cagctgattg gttacgtggg agatatcaca gcagaagata
    25381 ttgataagaa tccagaatta agcagtaatg gaaaaatcgg tcgctcaggt ttggagatgg
    25441 cttttgataa ggatcttcgt gggacaacag gtgggaaatt aagcattaca gatgcagacg
    25501 gtgtagagaa aaaagttctg atcgaacatg aagttcaaaa tggcaaagat atcaaattga
    25561 caatcgatgc gaaggcacaa aaaacagctt ttgacagtct aggaggaaaa gctggatcaa
    25621 ctgttgcgac aacgccaaaa accggtgatc ttcttgcgct tgctagctct ccaagctatg
    25681 atccaaacaa aatgacaaac gggatctcac aagaagatta caaagcttat gaagaaaatc
    25741 ctgaacatcc attcatcagc cgatttgcga caggttatgc tcctggatct acgtttaaaa
    25801 tgattacagc agcaatcggt ctcgacaacg gcactatcga tccaaatgaa gtgttgacga
    25861 tcaacgggct taaatggcaa aaagacagtt cttggggatc ttatcaagca acgcgtgtca
    25921 gtagtgatgt atcacaagta gacttaaaaa ctgctttgat ctattccgat aatatatatg
    25981 cggcacaaga aacgttgaaa atgggtgaga aaaaatttcg tacaggctta gataaattca
    26041 tttttggtga agaccttgat ttgccaatta gtatgaatcc agcacaaatt tctaatgaag
    26101 atagctttaa ctcagatatc ttgctagctg atactggata tggacagggc gaacttctaa
    26161 ttactcctat ccagcaagca gcaatgtatt ctgtttttac caacaatggc acacttgtct
    26221 atcctaaatt gattgcagat aaagagacaa aagataagaa gaatgtcatc agcgaaacag
    26281 cagtacaaac gatcgtgcca gatctgagag aagttgtgca agatgtaaat ggtacagcac
    26341 attctctttc tgctttaggg attccgttgg cagcgaaaac tggtacagcg gaaatcaaag
    26401 aaaaacagga tgtaaagggg caagagaaca gtttcttgtt tgctttcaat cttgataacc
    26461 aaggttatat gatggtcagc atgttggaaa ataaagaaga tgatgattca gcaaccaaac
    26521 gagcatcgga actattgcaa tacctcaacc aaaattatca ataaaaaatt gctttttaag
    26581 tacagtaaaa ggactgtggc gttatcgtca cagtcctttt ttcaaataaa cggttttcac
    26641 gatgtctctt tcacaacttc tttttgattt actcacactt cataaacttt ttttgtaaaa
    26701 tatttgagaa caagtggaga aataagtgta cttaataaaa tcacgataac taaaggtgaa
    26761 tagtactgtg gttcgatcaa ttgactttgc tgtccaattt gtaaaatgat caaagccatt
    26821 tctccacgcg aaatcatacc agcaccgacc atcaatgaac tatgttgaga aaaaccggca
    26881 atctgagcac caaaataacc tccaagcaat ttggtcaaaa tcgctacaag cgtcaatata
    26941 gaaataaaga gtagttgttc actaaagtca gaaaaatcaa cttctaatcc tacactgaca
    27001 aaaaatactg gtataaatac tgcatatcct aaagcctcta cattgtggta tacttcttct
    27061 ttaacctttg tctgactgac agctatccca gcaaaaaaag caccaatgac agagctaagc
    27121 ccaacaagat ccgctacgta agacattcct aaacaaataa ctagcgatac gatgataata
    27181 gaagaatttg cgtacacttt ttctgcaaat gaaagtagga aaggcgcaat ccatttgact
    27241 aataagaaaa taaaaacaaa ataaattagc tgttcaatca aaacaagcgg taaagaagca
    27301 ttgctagaag cttcccccgt tagaaaagat aggctgaaac ttagaactaa aacgactaaa
    27361 atgtcatcta ccacagaagc acccaaaatt gttgatcctt cttttgtatt cactacgttt
    27421 agttctttta atacttctac tgaaatactg acagaagttg ccgcaagaat aatcccaaag
    27481 aaaatcgctt catttcccga aacttggaat gctttgcctg tcaaccagcc aaaaacaacc
    27541 gggaagatga tcccaagcaa agcaacgaac ataccaggtc ggaaatattt tttcaaaaga
    27601 gataaatcac tttccagacc agctaaaaac attaataaga tcacaccaat ctccgaaaag
    27661 tcatggacta gaatatcagg tttgacccaa ttcaatccag cttggcctag caaaacacca
    27721 acaagtaatt gtccaatcac tgctgggata cctaatctcc gcgagatatg tgagccaatc
    27781 gttgttgaaa tcaatatcag gcataaaata ccaataaatt ccattctcat cattcctttg
    27841 tataaaatat agcccgaact tagcataaca tacattattg aaaaaatagg aaaatatact
    27901 cacaaaaaaa ccaacaatcc aaaagatttt tttggattgt tggtttttat acggtctatt
    27961 ttaataacta aaaatgatta acgtatagtc atttttctta ttctgatgaa attgataatc
    28021 tcctggatgc ccatgaaaat caaaataaag ccgaatattt ggaatagaag catcaaactt
    28081 ttgaatggat tgaacaataa gacgaatccg ccaatcaaca acagaatact actataaaca
    28141 ctccaacttt tagcttttgt attcatgctg aagaacaact ggaacaagct gttgatgata
    28201 acagctaatc caagaagaat cggcaggata gaaacgattg caggtgcaaa agcatagacg
    28261 atcagagcta agatcaggaa aaaaagacca ctgaacagac cgatactcca agaacctgtt
    28321 ttccttttaa aacggtaagc ctcaaaaaga ttcaacaacc caagcaaaac aagataggcg
    28381 gtgatcagat aaccgataaa atgaaaaaca gcagaagggt ttagcacaat cgcaatccca
    28441 gaaataatat aaacagcagc tcttaataag gcgtattttt gaatattttg tttaatcgtc
    28501 tcaaacatat aagaaaacct cctttgactt attttaacgt gaactagtca ttttaccaaa
    28561 ttaaatgtta caaaatgagc gaaggagaag aatatctctg gaaaaatcaa cttctgagga
    28621 tcttttccat ttttttgcct tttgctagct catcgatcat tttatctaaa taacgaattt
    28681 ctttcatcaa gttatcctcg atgtcttcta ctcgtacacc acaaacaaca cctgtaatca
    28741 aggaacgtga aggattgggg acaggggctt ccgcaaaaaa ttgttcgaaa gtcacttgtt
    28801 tttgtagttg atattctagt tctgtttggg aataacccgt cagccaacaa atgatttcat
    28861 ctacttcctc ttttatacga tcttttctct ccgcttttgc tacatacata ggataaacgc
    28921 ttgcaaaact tgtcgtaaaa atacgatttt gcgccataat cagtcacctt tctttttttc
    28981 aaattattta ctacttatag tatataagaa aaagagaaga aaaagcgatt attcaaaaaa
    29041 acaaaaagga ttgtgacaaa attcttgtca ccatcctttt ttccgctctc tgacttttta
    29101 caatcttcat agctcaagtg cgcatcagat aatcgaatgc tcctaaagct gcagtagctc
    29161 ctgaacccat tgaaacaatg atttgtttgt atggactatc tgtacaatca ccagctgcga
    29221 aaacgccggg gatattcgtt gcgccatttt tctcggtcac tatttcattt cttggattca
    29281 tttcgatcgt tccatctagc caatccgtat tagggagtaa gccgactaga atgaatactc
    29341 ctgaaacagc tagtgtatgc atctccatcg tacggcgatc gatataggag agagattcta
    29401 cctgattcgt accgctgatt tctttcgtcg ctgcatgttt gataacagtg acattcgcaa
    29461 gagagactag tttatcctgt aagatttgat ctgcttttaa ttctggtaag aattctagaa
    29521 catatacatg ttttgctaac ccagccaaat cgattgctgc ttcaacacca gaattaccac
    29581 caccgataac agcaatctct tttcctttaa ataaagggcc gtcacagtgt gggcaataag
    29641 caattccttt atttttaaac tctttttcgc ctggtacatt gatagaacgc cagtgcgcac
    29701 ctgtcgcgat gatggcggtt tttgctttaa gtgatatacc gttagctaag ccaatattaa
    29761 taaaatgttg ctctttattt aaggaaacgg cctgttgatt catcataatg tcgatagggt
    29821 aactacgcac atgactttct acttgctgca tcaattttgg accttctgtg taagaggtac
    29881 caatcatatt ttcaatccct agtgtttcaa cgacttgtcc gccgaaagaa tcagcgacaa
    29941 gtcctgtacg gattccttta cgtgctgcat agatagctgc actactagct gctggtccac
    30001 cgccaatcac taatacatca taaggttctt tttcaaacaa ctcttcttca atcaaagggc
    30061 cagtgatttt ttcaacaatt tgttcgatcg tcatgcgtcc gctactaaat tcttctccat
    30121 ttaaaaacac agttggcact gccatgattt ttttatcatc gatttctttt tggaacatcc
    30181 caccttcaat catcgtatga gtaatatttg gatttaatac agctaatata ttcaatgcct
    30241 gtacaacgtc ggggcaatta tgacaagtca agctaacata actgtcaaaa tgcaatgttt
    30301 gtgttatttt tttgatttta agactcaaag attcactgat tttcggtgcc cgtccactaa
    30361 tttgtaacat agccagtaaa aaagaggaaa attcatgacc gagggggata ccagcaaaaa
    30421 cgattccact ttctttgcca actggattga tagaaaaact aggtgtgcgc atcgaatctc
    30481 ttttttccca agagatttta ggagacattt ctgctatttc cttcgtaaat tcagctagtt
    30541 tttgactatt ttcactatta tccaaactaa cagaaaaaac aataggagat tcaatcagtg
    30601 ctaaatattg ttcgagattt tgtttagttt ctttgtctaa catatccacc acctaaatct
    30661 ttccaacaag atcaaaactt ggtttcaatg tttcgccact ttctttccat tttgccggac
    30721 aaacttcacc tgggtgggta cgtacatatt gagcagcgcg aatcttatca atcagtgcac
    30781 tagcgtcacg accgattcca tctgcattga tttccatagc ttgaacgatt ccgtctggat
    30841 caataataaa tgttcctcgc tgagctaatc cttgctcttc atccaaaaca tcaaaaatac
    30901 gagaaatctg atgagacgga tcaccgatca tcacgtattc gattttaccg atagcatctg
    30961 atgtatcatg ccatgctttg tgagtaaagt gggtatcggt agagcaagaa aagacttcaa
    31021 cacctaaaga tttcaatgta ctgtattgtt cttgtaagtc ttctaattca gtaggacaaa
    31081 caaaagtaaa atcagctgga tagaagcaaa gaatactcca gttccccata agatcttttt
    31141 cactgacttc taaaaattct ccttgatgat aagcatgtgc attaaattct tcgatttttt
    31201 tcccaatcaa tgacatagta aaacctccaa taataattat ttatttataa ttattgtaat
    31261 ttagttggag ttgcaaagtc aatggatatt ttgttattta ataaagatta ctcaaaaacg
    31321 aaggttaatt ataaaaatag aagaggtcgc aagaaaatgc tactgctgga aatactcggg
    31381 ataacgttga gtaacagttt cctgttcttt cagcatcatg tgtaagtgca atcccgtttg
    31441 aaatcgagct tcttctggtt ttttatcaac taaagccttg agtaaatcat aatgttgttc
    31501 catgatcgtt tcccatggaa gcccctgaat ggtcactcgt aaccagcgga aacgttctaa
    31561 atgggtatta tggtcatcta accagttcca aacttctttt cttcctgcaa tttcgaaaca
    31621 agcttcatga aataaattat ctgcacggaa aaaagcgcgt tcgtcattta ttttgactgc
    31681 tttttcttgt tcttctaaaa tcgtttctag cactattcgt gtttgtgtgt ccaatttgga
    31741 acaacattca atcatgattt gctcttccaa ctgttctcgg acaaagcgag cattttctgc
    31801 agaaacaaga ttaattttgg aaacatacgt accactttga ggtatcgtat acacgagttc
    31861 ttgctgacgt agttgaatca atgcttcgcg aatgggagtc cggccgatag caagatgttc
    31921 ctccagtgcc ttttcagata ttttccttcc aggttctaag tcagcataga taatttgttt
    31981 acgaatcgct tgataagctt gtgattgcaa gctgcttttc atctttattc ctccaaaatc
    32041 atttctatga acgtagtata acagaagagg tctgaaaaac aatctggtat gtcacattaa
    32101 ctagtaaatt tgtataaata gtaaaatttt ggttgacata gcgattttta ttgcataaaa
    32161 ttcacttgaa tacaaaaact aatatgttag tttaggaggg gaaaaatgat tgatttaaac
    32221 aactggagga actttatccc tgaattatct caaaaagaag tagaaatacc tcattttgac
    32281 cgtgcatcat taagagaaaa tggacaaaaa aatcctgtct ggatccattt cggtggcgga
    32341 aatctttatc gaggatttca tgcagaaatc gctcaacgtt tagcggatct aggcgaattg
    32401 tccactggtg tcattgtttg tgaaacattc gatgaagaaa taattgaaag ggcttaccaa
    32461 gattttgatg atttgattct tgaagtggtc atgccaagca gtggagagtt cgtcaaacgt
    32521 atattagctg cgacagctga cagtctgtat tgtcatccag attgttctga acactttgat
    32581 aagatgattc gacactttga aaatcctagt ttacaattag ttacttttgc tattactgaa
    32641 aaaggttatc gattgaaaga tacaaataat aaatatatag gaccgattgc agtcgatata
    32701 aaaaatggtc caaagaagcc atcacataca atgagtattc ttgctgtgct actttataaa
    32761 cgttttttat ctggagcttg gccgattgca cttgtctcta cagataattt ttcacaaaac
    32821 ggtagtcaat tacgtacttc tttgctaacg atcgcaaaag aatggcaaaa ggaaggttat
    32881 gtacaaaata catttatcca atacttatcg aatgaagaaa ttgttgcgtt tccatggacg
    32941 atgattgatc gtatcacccc gaatccttcc ttgtcagtta gcaaaatgtt ggcaaaagag
    33001 ggcttttcaa atatgtcgat tttccaaacc gcaaaaggaa ataatattgc tccatttgtc
    33061 aatacagagg acatccatta tttaatcatg gaagatcatt ttcccaatgg acgtccgaaa
    33121 ttcgaaaaag ctggggtatt cttgactgat cgtgcaacgg tagataaagc agatacgatg
    33181 aaggtgacga cttgccttaa tcctttgcat acgtctttag ctattatggg ttgtttactg
    33241 gggtatagga gcatagctga ggagatgaaa gatccagatt tatcgctgct agtttctgaa
    33301 atcgggtaca atgaaggcct cccagttgtg gagtctccag gtatcattga tccaaagatc
    33361 tttctggaag aagtaattac tgtgcgcttt tctaatccaa atattcctaa tatgcctcag
    33421 cgaatcgcca cggatacctc tcaaaaggtg gcgattcgtt atggtgagac gattaaaaaa
    33481 tatatggaaa tagaagttaa agacccgcag acacttgtat tcataccatt gactctcgct
    33541 gcttggcttc gataccttat tggcattgat gatcaaggaa aaccattcat cccgagtcca
    33601 gacccactat tgacagacct tcagcataag ctttccgata ttcaactggg tacgcaagat
    33661 acatcacata ttcatgagaa aatggctcct attttgtcaa ataaagaaat ttttggtgta
    33721 gatctttacc aagcagagct tggcagaaaa atagaagaaa tgttcatcaa gatggtagca
    33781 ggaccaaatg ccgtacgaaa aacattacag gattacttac aagaacatgg aggaaattta
    33841 tcatggaaat gacttttaga tggtacggat cacaggaaga aaagattacc ttagatgaaa
    33901 tcagacagat tcctgcggtc aaagggattg taggcacatt gatggatata ccagtaggag
    33961 aggtatggcc tagggagagg atcaaagaac taaaagaaga aattgagtct cgtgggttaa
    34021 cactaaaagt aattgaaagc gtcaatgtgc atgatgccat taaaattgga ttaccttccc
    34081 gtgagcagta tatcgaaaac tataagcaga caatccgaaa cttggcagaa tatggaatag
    34141 aagtcatctg ctataatttt atgcctgtat ttgactgggt gaaatctgac cttgactacc
    34201 gcttagaaga cggctcatcc acgttagctt ttatatctga tgatattcca gatgatccaa
    34261 gagaaatcgt cgcgcgcatc gaacattcca gcaatgaatt tggattgccg ggatgggaac
    34321 ctgagcgctt agctcctatc aaatccttgc ttgaagccta tgcatcagta gacgaagaaa
    34381 aacttcgaga aaattttgcc tattttctcc aatcaatcat cccaacatgt gaagaggtag
    34441 gggtcaaaat ggctgtacat ccagatgatc cgccatactc gatttttgga ttagctcgaa
    34501 tcgtaaagaa tcgagaagat ttagattggt tgtgtcgcat agtagacagc ccttataacg
    34561 gaatcactct ttgtacaggg agtattgcag aagatccaga aaatgatgta tatgggattt
    34621 tagcagaatt ttgccaaaga gatcgaatcc catttgcaca tgttcgaaat attaaatttt
    34681 tagctaatcg agatttctat gaggcaccac ataaatccga atacggttca ttagacatgt
    34741 accgcatact aaaaacaatg tatgacaacg gatttaacgg atacatccgt cctgatcatg
    34801 gaagaatgat ctggaatgag acaggaagac caggatacgg cttatatgat cgagcattag
    34861 gtgctgttta tctaaatgga ttatgggaag caatcgaaaa aggaaacaaa agtaaagata
    34921 attagtgaaa aaaactgtaa ttttattatg taaataagaa ggatgttcaa attcttgata
    34981 cataaatccg gaggttgtta ataagcattc ggatttatgt atcttatttg gtctaggaag
    35041 taacttgatt ttcttttttc gattgatcca tgagtgcttc ttcttttaat tcaaagatat
    35101 attctgagat cctcataatc acttttttcc agtcttctgg tgtgatgatt ccttttaaaa
    35161 tatagatttt tttgatatga gaaggcaatt gtttcaaagg gcagtcgctg ataacgatgt
    35221 tgcagttctc cataaaatca ttttgtattt tgatcgattc atttgaaaaa atttgttcta
    35281 tcttaacgat cagatcttta ctgatataaa aatcacgcgt atagttcaca ccaatcacaa
    35341 ttcgcggctg ttttttgtag cgatcataca aatgtactag gtcttcaacc atccattgca
    35401 cattttcctt ttctaaaata actggtaaca agtgttgatt ttgttctctg aaatccatat
    35461 aaaattgttc aatggcggtt tggacttctt tcaatacgct atgttcttga taaagcatga
    35521 aagtaggaag gggcgtattt ggttgattga agttaagaaa tccattataa agtttatttc
    35581 gtgcgaatgc caatgagtaa agaatctttt ctttttcagg tataaacaaa tcaaatacag
    35641 tacaaaggga ctccaccatt tctcttgaat aaagaacatg aggaacgttt gcttttttta
    35701 aaagtttgta ttgctccaag cttgtttctt ccgattcgat tttcgtgata gaaagtcgag
    35761 ctaaaatatt cactaagatg cgttgttcgc gacaaattgg cagattaggt cgcaaaatat
    35821 ctacagagag atcaataaaa aagtcaatat attcatactc tgtgaattct ttttgtacgt
    35881 ctttcacaga agttgcagga aaattctctt tacatatttt taatagaaaa tataatttat
    35941 ctatcatacc gctatttagt cggtcaagta cttctttttt aaaaaaagta ttcatcataa
    36001 aagccactac ttgcggatct tcatcataaa attctgtagc agtacttgaa aagatgttcc
    36061 aataaatatc taatgctgca tattgaattt gtacctcttt tccagaaaaa gactttctgc
    36121 cattattaga aaaggagata gacaaaccgt actatgctaa aaatgcatta atctttttca
    36181 ttttcgtata caaataagag gcactatagt ttgtttgctg actgaaactg ataatcgaat
    36241 ttgtctgaca gctgaacaaa gaagtcaaga tctgataact gactgattcg ttgcagtatc
    36301 ttccaaatag acgataatag cagtaagccg gatcaattcc ttcaaaattc aactttaaaa
    36361 aaggtttttg cttatcaata tgcagttctg ttagattgag acgctcaaag tcttgttcta
    36421 attctttgat gtatagtaaa aaatttgatt cagacatatg gaaatgatct gctaaatatt
    36481 gtgtagtaat aaagaaacga tgttcagata ggaaaagaaa aatatctaat tttgtattgc
    36541 tttttttctt caagataaac gttttaatcg cttcttgagt ttgcatgaaa gaggctcctt
    36601 tttacttaaa ttgttaaaac ttgaaaatat tactgttttt ttcataaatt tagacaaaaa
    36661 aacagtaaac atataggtag aatctctact atcttatttc ttgcatacaa aaaaatcaag
    36721 gagtgagaga gttatgagat attcaaccat cttaatatca acatttgcag ccttaagctt
    36781 atcgcttact ataaatcaga cagtccacac agaggagaca gaaacagcca aatggacaga
    36841 aaccaagtgg gccaacgtca atcgaattac ctttcaggat gaattttcat ccttcaattc
    36901 aggagtcgca aaattaagaa aaggacaaac agcgaccatc aaagcaaaaa ttgactattc
    36961 gggagactta gcacctatac tacaatcagc tgttacattt gagaatgttg atcccacttt
    37021 gtcgattggc tatgacgaga acactttgac tatccaacac aagcaagcga tatttacctt
    37081 caccgttaca ttaaatcagg acttgttaaa accagcgctt tttactgtca aggtatcaga
    37141 tggactaccg aacgatcatc atttaacgcc gtatagtcag cggcagacaa ttgagcaaga
    37201 ttcagccatc gactcaggag gagatcttgt tgaagaacca acagataaac cagaaaatga
    37261 aaataagcca gaagtaccgc ctacagaaaa tccagacgga gaacagaaac cggaaataga
    37321 acctggagaa gaacccgata cagaaactca accagagcca gacaataaat caaaacctga
    37381 aataacccct ggagaaaaac cagatgtaac tccagaacca gatacggatt ctggaaacca
    37441 aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag aaaaaccaga
    37501 ggtagatcca gaagaaaaac cagatgtgac tccagaacca gatacggacg caagagacca
    37561 aggaatacca gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa aagagagtaa
    37621 aaaatcaaat ctagcaattc taaaaataaa tgaagagcag ctgaataaaa aatcacgaat
    37681 atttgatagc gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa cctttgctgt
    37741 cctgaaacga aaaataaaca gctaccaaaa tccggagagt cacaaaacaa agttatactg
    37801 tggagcggaa tcattctatt aagtatagca acaatgcttt ctgcaaaaag atttaaacaa
    37861 aacagatctc tctaacattt gaataatgtc tgtgaacgca tttttcaaat aaaaagaaat
    37921 atccagaaaa tgaccccaaa ataccagatt gaaatctgat atttggggtc gttcatactt
    37981 tatctttctt catatttatt ttaagggata tctgtttctt ttggaagatg gttcgggatc
    38041 tttttcactt tcaatacatc taaaaagaag gtaaagatcg gaatgccgac aatcaagccc
    38101 caaacgccaa agaaccgttc gctaacaagt aagaccacaa atgtataaaa aatcggcagt
    38161 tctgttttgc tggacatcaa cttcggattc agcacataag actcaattag atggacaatc
    38221 aaaatcgtaa tcaggatata aacaacatct cggatgcctc cttgggaata agctatgaaa
    38281 gatagaggaa tacaagaaat gataacacct gctactggga tcagacttaa aatgaaaatc
    38341 atgatcgcta aactcagcag ctgatgaaag ccaaatacag ccaaggcgat tgtagtgagc
    38401 aacgtattta aaagagcgac aacaaattga gcttctaata ccaaaccaaa tgtattgaca
    38461 aacttatcgg caaaatagta aatatcttga aagaaccaag aaaattcact ttttaaaaac
    38521 aatcttgaga atacaattgt ttttttcttt tcaatcataa aaaagaaact taagatgaat
    38581 gacatagcaa acgccactgc catttttcct atatcctgta aatagccaag cgcaatcgaa
    38641 gcgccatttt gaagctgttc aaaaaaatcg tttcgctcaa tataagtatg gatatattgt
    38701 aaaacaggat ctgcatcttt tggctgatgc tcgtaaaaat taataacgga ctggatcatg
    38761 tcaaatgttt gatgaataag catcgggata tactttgtga atgccagata gataaggtaa
    38821 acgatcagtg tataaatcat gatagctaaa acacctgtag ggattcggac aaatcgctga
    38881 atgatttgaa caagatgaat catcaaataa gtaaagataa atgtgagcaa aatggttgta
    38941 atcatacttc taactaagaa aagaacaaaa acgacaaata ataaaacagt gagacggcgt
    39001 attttttcat tcgccaaaaa acgctgataa acggtcaaat cgacaacctc caaataacaa
    39061 aaaataaaca cgttattcat tatatgatgg aatatgaaaa taagaaagag caaaaagaaa
    39121 aaccgctaaa tgacgttatc ttatgagttg gttttcctca taaaatcgtt catctagcag
    39181 tttcagcctt ttttctggtc ttgatgaaaa ttatctagca tttttcgggt cagatgttgc
    39241 agtgtatcca attcttcctg ttcaagtgga agataagact tgatcttttg tggaatacct
    39301 tctgcttgtt tttgcaacgc agcaccttga gcagtcagcg taacatttac gattctttca
    39361 tcacccgttt cacgctgacg attaatataa tttttttctg ctaacttttt tagtaaggga
    39421 gtcagcgtac cagaatccag acacaaatca tttcctagct ctttcactga agtctttttc
    39481 ttttcccata aaactaacat cacaagatac tgggtatagg taagattcag cggcttgagg
    39541 aggggagtgt agtgtctaat gatttcttta gaggcggcat ataaaggaaa acatacttga
    39601 ttaactaaat atagatcttc catttttcca cctccgctgt tttactatag tgtatcatag
    39661 cggatttaca caaaaaagtc tattcactaa ctgctttcag agtaacttct atatttccag
    39721 ctgtagcttt tgaatacgga cacactttat gcgctgcctc taataattct tgcgtttctt
    39781 ccagagaaag accttcgata gatccttcaa tctctacacc taagacaaag ctcgtttcac
    39841 tttctgaata aagggaaact tgtgcactga cagtagaagc cccagagata tttttttgct
    39901 gtttgatcaa ttctaatgca ctgttaaaac atgcactgaa accagctgca aacaattgtt
    39961 cagggttggt tgcattttcg acgcgtttac caggagctgc aatattcaat tcgaatgtac
    40021 gatcgggatt atgcacttct ccagagcgtc cgcctgtgtt gatcattttc gtagaataca
    40081 tttttttcat aaaattacca cctttaatta tattttatac aattgaattt tacacaatat
    40141 aattgtggaa agcaaagaaa tggattggca taagataaat aagagaaagc acatgaaaca
    40201 gacgcgcttt ctcgaatagc aaacatatac aaaaaatatc agagacaaaa aataatctca
    40261 tcataaacat tacatatatt tctcaaaaag ttgttttctt tcttcaaaaa ttacttcacg
    40321 atggacctga tgaagcatcc ataaataacc aaaaggatct ctgaacattg agttagtcac
    40381 accaaagtcc atcatttctg tcatttcttg aatctctaca cagccatttc gtaatgcttt
    40441 attatgtgtg tcctcgatat cagagacgat gatattgaac cagatcgtat ttggatgttc
    40501 agcagtagga gcagtcaaac caagagacgt gttttcatcc agcatatgga atctcgtacc
    40561 atacaaggta aaaatgactt cattattgcc tctagggtaa tctgttgcct caatcagttg
    40621 tatttcatca aagattgttt gatacagttc tagtgctttc aaactatcat ttaatacaaa
    40681 atcgatttcc gttcctttat gcataaagat ctcctctcct tttcagtcga acagttacta
    40741 gattctttta ttataccagc taaagaaaac gcattcaaaa aaatagcgga tttacttacc
    40801 aactcattcg attggatgat aagtaaatcc gcgtcccatt acttctgcta cgctgctgac
    40861 aatgacaaag gcacttggat cgatttcttc tatcaatggt ttcattcggg aaaattcatg
    40921 atcagtcaca acgatcaata aaacctcttt tgactttcga ttatacccgc cttttgcctc
    40981 tagcagggta aggccacgtc caatctcagc acttaagcga aggcggatgt cttccaaata
    41041 atgttcactc atgaccataa cagatttctt tcgattgata cctgtttcga tataagtcat
    41101 gacaattgaa gtgattgcaa tagataaaat agcataaaga aactcttcta ttccaaaaat
    41161 aaacagatta agagaaacaa cgaatgcgtc tgtaaaaaat aagccaatcg aagtattcaa
    41221 gtggaaatat tttttaaaaa tgaggggagg gatcgtcgta ccgccgctag aagagttgtt
    41281 tttatataaa atcgcaactc ctaaagcaaa aatcgcactt ccaaaaatga cagacaacaa
    41341 ccgatctgaa gtaatcattg tttctggaac tattgcaatg attaaaggaa aaaccaagct
    41401 tccgaaaagt actttaaaaa agggtttttt acctaaaaat agaagagcta gtaccaacat
    41461 aacgatattc aacactaaaa taactatagc tcggtcaaag cctaaagcag cttccagtaa
    41521 tattcctaga ccgctgacac ctccagcagc aatatgatga ggccctaaaa acatattgat
    41581 agcgactcct aatatgaaga gggcaaaaat aatcttaatg tatgtccata taaattgtct
    41641 cgtttctttc atttattcac ttccttaatt cattaataat actcttatct tattataact
    41701 tacttttcac cttaagttaa ctttaattaa taaaaaaatt aggctagtct aaattattat
    41761 gtttcattct tctagaatat acttctacgc caaaaactgt taaaaaactt ttactttaga
    41821 aaattggaga aattgatcgc ttaagataaa ttcaggacac taaatagtac aggaagtaga
    41881 ctaaaacttt taaaagtttt aagtctatct atttggcaag tttaaaaaat aaaaattcgg
    41941 tatcaaaaaa ctgttaccga taacattttt gaaagcgttg tcttttgctt ctctatcact
    42001 tatagtgatg acgtcatatt aaaacacaag gaggaaatca atggatacag ctgcaattta
    42061 tcatcgaccg gaaagtgaat atgcatttct atatacgaat acaagatttc gtattcggct
    42121 tcgtactagg aaaaatgata tcaaaaaagt atatgtgcta tgtggagatc catataccat
    42181 aacaactgaa aaatggtatc aaaaacaacg accaatgaaa aaatgtctaa gtaccaatgt
    42241 tcatgattat tgggaaattg aagtgacgag cgaaacaagt cgcttacagt atgcgttcca
    42301 tgttgttggg gaagataaca cggactgttt ttacggtgat caaggaattt ttccttatcg
    42361 agaaaatgtg attactgaac caaattttta ttttcgaata ccttattttc atcaaatcga
    42421 tcgattcact attccagaat gggtaaaaga aacagtatgg tatcaaattt ttcccgaaag
    42481 atttgcaaac ggggacaaag caaatgaccc cgaacacact ttaccttggg gaagtaaaga
    42541 tccagaccga gaagactttt tcgggggaga tttgcaaggt gtgatggatc atttggatta
    42601 cttgacggac cttggcatca atggcatcta tttttgtccc atatttaaag ccacatccaa
    42661 tcataaatac gatacgattg attattatga ggtggatccc gcttttggcg ataaaaaact
    42721 tttaaaaaac ttgattgatg aagcacataa aagagggata cgtatcatgt tcgatgctgt
    42781 attcaatcat atgggtgtcc attcaccaca gtggcaagac gttctcaaaa acggagaaaa
    42841 atcgatctat aaagactggt tccacatccg ttttttccca gtagattctt atcagatgac
    42901 tgacttacca gaaactgctg aaaatatccc atatgatact tttgctttta ctccattcat
    42961 gccgaaacta aatacagcaa atccagaagt gcaaaaatat ttgctggata tcgcaactta
    43021 ttggataaaa gaattcgata ttgatggatg gcgtcttgat gttgctaatg aagtcgatca
    43081 tcatttttgg aaaaaattca gagaagcagt tacagaaatc aaaccagaca tctatatcct
    43141 tggagagatt tggcattcct ctcaagcttg gctgcaaggg gatgaattcc atgcagtgat
    43201 gaattatgca tttacagatt cgattaaaga ttattttgca aaaaagaaaa tcacagcttc
    43261 acaaatggtc agtggaatga atcatcagca aatgctttac agagatcagg tgaacgaagg
    43321 aacattcaac ttattagatt ctcatgatac tgccaggatc ttgacccttt gccaaggtaa
    43381 caaagaattg atgaagtctg tgatggcctt tatgtttctt caaaaagggt ctccttgtat
    43441 ctattacggg acagaaatag ggatgactgg tgaagatgac ccggattgcc gcaaatgtat
    43501 gatatgggag aaggaggaac aagatctaga gctattcggt tttataaaag aacttgtttc
    43561 tctaaggaaa cagttgtcta agattatttc tgaaggttct actcagtggc taatagtaaa
    43621 tgatcgtgaa gataagctct atttcacacg gaaactagag ggacagatca tctatgttta
    43681 ctttaatcag tcaaaagaac cttgggtagt agaacaggaa aatgaggtga ttcttagtca
    43741 aaattgtcag ttgttagaag acgggaaagc agagatacga caatacggat ttttgatcat
    43801 gtgttgaaaa ctgttaccgg taacaacaag aaagcgtttg caaatgaaga tttgtgccca
    43861 ttataataag ggtgtaacag cacgttgaat acaaaggagg aaatgaagat gaaaaaaaga
    43921 agtgtgaaac agttgggttt agggttaatc tctttaggaa tcttaggcgg attagcggct
    43981 tgtggcaatg gtaactcttc agcagaaggg gaagccgatg aaggaaaaac gttaacagtt
    44041 tccgttgatt ctggatataa agattatata aacgaaataa aaaacacttt cgaaaaagaa
    44101 aacgatgtga aaatcaagct agtagaaaag gacatgttcg atcagttgga atcattagcg
    44161 ttagatgggc ctgctggaaa gggacctgac gttatgatgg cagcatatga ccggatcggt
    44221 gcgttaggac agcaaggaca tctggcggaa gtaaaactgg gaaatgaagc agcttacgat
    44281 gaaacagaca aagcccaagt gacttatgat ggaaaaatct acggagaacc tgcagtaatc
    44341 gagacccttg ttttgtacta taacaaagat ttagtcgata cagcaccatc aacgtttaaa
    44401 gatttggaga atctatccaa agacagtcgt tttgcttttg agtcagaagc aggaaaaaat
    44461 acaggatttt tagctaaatg gactgacttt tattattcct atggtttaat tgcaggttac
    44521 ggtggctatg tatttggtga tgatggcaca aatccttcag atatcggttt gaataatgca
    44581 ggtgctgtag aaggaatctc ttatgcaaca gattggttca aaaatgtttg gccaaaggga
    44641 atgcaagaca taaaaagtgc aggagacttc gccagccagc aattcatgtc caataaaact
    44701 gcagcaatca ttgacggtcc atggcaagcg caaacataca aagaaaacaa tatcaattat
    44761 ggtgtagcca aaattcctac tctaaataat ggacagcctt atcaaccgtt tggcggagga
    44821 aaaggatggg tagtcagcaa ttatgctaaa aacaaagacc tctctcaaaa atggcttgat
    44881 tatgtaacga atcaggaaaa tcaagaaaaa ttctttgaaa tggtaaatga aatccctgca
    44941 aaccaacaag ctagggaaac agcaaaggct aaaaacgatg aattaacaac agctgtgatt
    45001 gaacaatatg aaacagctca agcgatgcca aacatcccag aaatgggcga agtttggaca
    45061 ggtgcagaaa acttgatgtt cgatgcagca tcaggttcaa aaacaccaaa agaatccgca
    45121 gatgaagcag taaaaacaat cagtgaagcc atagaacaaa aatactgatt aaaatgataa
    45181 ccgtccattt ttataaaaaa tggaacaggt gcttcagata aaagtggcaa gagcgagatg
    45241 gaaaattatc ttgctcttgt tgccattatc ttaaaagcag tttgcggttt agcaagttta
    45301 gagggggcat tcagatgcag caagataaaa caagcaatat caacgaaaaa aaaatcacgc
    45361 tttgttcact tattccaggg atggggcagt ttgtaaatgg ccaagctttt aaaggtatcg
    45421 tttttttcgc catattcgtc ctattcattg gtcagatgct tctgggcgga tttcatgcat
    45481 tggtaggatt ggttactttg ggaagtatac caatggaaga tcattcatta tttattttga
    45541 ttgaaggcac tttgcaattc atcattacaa tcattttctt gattttttac ggtttgaatc
    45601 ttttagacgc ttatcgagta gcgaagatgc gtcaagaggg caaaaaaatc aatcggacga
    45661 tgaaggaagt catacataat ataggtgatg aaggttttcc ttatctattg acacttcccg
    45721 cttatcttct catgattttt acgatcattt tcccagtcct agttacattg ttcactgcat
    45781 ttactaatta tgacttcaat catattccac cagccagttt aattgattgg gtaggggcta
    45841 aaaacttttt tagtatcttc tttttaagct catatcgtaa tacttttttt gcagtctttt
    45901 cgtggaccgt gatttggact ttatgtgcaa caactttgca aatcaccctt ggtattctca
    45961 cagcggttgt tgctcaccag aaatttatta aattcaaacg tattttcggt atcattttct
    46021 tattaccatg ggcggtccca gcttttatta ctatcatgag tttttcgaat atattcaacg
    46081 atagcgtagg tgccatcaat actcaagtga ttcctttcat aaaccagctt ccttttgtag
    46141 atatcagtgc aatctcttgg aaaacggacc cgaactggac gaaagtagca attatcttag
    46201 tgcagggctg gttaggattt ccctatatct atgtgatggt tacaggaatc ttacaatcga
    46261 tttcagaaga cttatatgaa gcagcaaaaa tcgacggagc aaatgcatgg cagcgctttt
    46321 caaacatcac gcttcctgct gtcttcctag tggcagcgcc aacatttatt acacagtata
    46381 cggggaattt caataatttc tccatgatct atctgtttaa caatggcggt cccggaagcg
    46441 taggcggagg agctggttct actgatattc ttatttcctg gatctataaa cttacgactg
    46501 gaacttctcc gcagtattct atggcttcag caatcacgtt gattatctcc gtcatcgtta
    46561 tctgtgtgtc attgctggta tttaaaaaga caaatgcgtt caatatgggg gattaacaat
    46621 gaaaaaattt cattcgagtg ctcgattcca aaaacgactt tctcactttt ttacttactt
    46681 ttttctttta atactgtcag tcattatcat ctatccgctt ttgatcacag caagtactgc
    46741 cttcaagtca ggaaatattg cagcttttag tttagatctt tcaagtgact ggactttagg
    46801 aaactttact cggctattca gtgaaacact ttacgggact tggtataaaa atacattgat
    46861 catttctgtc ctaacaatga tcgtacaagt gactgtagta actttggcgg gctatactta
    46921 tagtcgctat cgttttatcg gccgcaaatc aagtttgatg ttctttttag tgattcagat
    46981 ggtaccgact atggcagcat tgactgcctt ttatgtcatg gctttcttat tagaagcgct
    47041 cgataagtac tggttcttga caatgatcta tataggcggt ggaattccaa tgaatacttg
    47101 gctgatgaaa ggatactttg acactgtacc aatagattta gatgaatctg ctaaacttga
    47161 tggggcaggg catttcagga tttttttcca gattgtatta ccgcttgtca aaccgatgct
    47221 cgccgttcaa gcattatggg cttttatggg accttttggg gatttcatgt tagctaagtt
    47281 cctgcttcga acacctgaaa atcagactgt tgctgtcggc ttacaaacat ttatttctga
    47341 tgtgacaaac caaagagtct ctctttttgc tgctggggca attttgattg cactaccgat
    47401 ttgtctatta ttcttctttt tacaaaagaa ctttgttacc ggcctgacat ctggcggaac
    47461 aaaaggatag gagcagagta tcatgcaaac atttccgtta aactattttt ccgcattacg
    47521 tacgccaact caagtgtttg cgggacgcaa actattatca tggcctaaat ttttcttgat
    47581 atttgtattt ttagtttcct tgatggtcat gcctgtgact cttttttatg ccaatcagat
    47641 ccaagcgatt ccattagaac aatttctatc cgttcattcg ctgatcgatg aacaaggaac
    47701 ccaaaaattt tctgaacttg aattatctga aactgggtta caagcatcgc aacaaacgat
    47761 agctgtgacc cctgaaattt tggtaggtgt gtctttatct gagaaacaac aatccgatca
    47821 tggaactttt attgattttg agaaggaaca atgggtgatc caacaaaagg ataaatctgg
    47881 tatacgccgc tatacgatga attatagccc atctttccaa ccagattctg tacgtacgcc
    47941 cgaagatttc caacgatttt tagaaagaga attttatgcc agtaatcgtc caacaatcat
    48001 tttatcttat agtttaagtc ttggtcttgt cctatttgtc atgacgagtc tcattttatt
    48061 cggggcttcc tttttcttgt ggatgacaag aaaaagccaa ctttcatcca ttcatacatt
    48121 caaagagagt gcaaatttga tgctgaatgt tatgggaata ggttccatgg cagcagcagt
    48181 tgtagggttt atccactttg actttatctt gatgctaagt gtccaaacaa cgataactgt
    48241 cttgctgctt ttatgggttt ttgtcaaaac aagattcaaa gataagcgtg tcgaatgaca
    48301 agtttaagcg atttatgcga caatgaatgt gaaaaaaatg aaaaataaag tagtttttga
    48361 aaaatttctg aaaaaattaa acttcttagt tgattctttt cttttttagt attaacgtaa
    48421 tagggtagtt tttttaagga ggaaaatcat gttagagaaa actttataca atcaattatt
    48481 ttcacgttct ttctcgctcc ctgtcgaagt aaattattgg gacggtacaa cgaaacaata
    48541 cggagatacg gacactccta cccaaatcaa aatcaatatc catgaagaaa tcccaatgaa
    48601 agagattaca agtaacgcat ctttggcact aggagaagcc tatatggatc atcgaatcga
    48661 gatcgaggga agtatccaag cattgatcaa cgatgcttat cagcaagcaa acagcttttt
    48721 acgaggaaaa gattatctaa aatggctgcc aaagaaagaa aaacatacga aacaaaaaaa
    48781 caaagaagat atccatgcgc attatgactt aggtaacgac ttttataaac tttggctaga
    48841 tccaactttg acttattctt gtgcttattt caatacacct gaagatacgt tggaacaagc
    48901 acaaatcaat aaagtccatc atatcttaga caaactcttt atcaaagaag gcgaaacgtt
    48961 gcttgatatc ggttgtggct ggggaacatt gatgttcact gctaccaaag agtataatgt
    49021 caaggcaact gggatcacac taagtgaaga acaatacgat ttgattacca aaagaataaa
    49081 agaagaacat ttagaagata aatgccgtgt attattaatg gattaccgcg aactaaaagg
    49141 cgaaacgttt gatcatatca caagtgtagg tatgttcgaa catgtaggtt ctgaaaatct
    49201 tgaaggttat ttcaaagtgg taaaagattt attgaaacca aaaggaacag ctttgatcca
    49261 tggaatcagc cgacaacaag gcggtgcgac aaatgcttgg atcaataaat atattttccc
    49321 tggcggttat gtccctggtg ttactgaatt agttgataat attacaaaaa acgatttgca
    49381 actgattgat ttggaaagct tacgtcgtga ctaccaattg acattagaac attggacaaa
    49441 gaattttcat caagtccaag atcaagtcac gaaagaaatg ggtgattcct tctatcgaat
    49501 gtgggatcta tatttacaag cttgtgcagc ttcatttgaa tcaagtaata tcgatgtgat
    49561 tcaatatttg cttgttcaac ctggaaacaa tgacatccca atgcatcgtg cataattgag
    49621 gcaaaagtac tgtttaaaga ctatttaaaa agaagctaag ttttatgatg ctccagaagt
    49681 gaactttttt gtctaacttc taggattgtc tctaaagctt agtttctttt tctttatatt
    49741 ttcataataa aagtttggtt ctttaatttt taaacatgac agaaaatttt ttcttcgtta
    49801 tacttgtaat agaatactat tttataacaa aggggatcga atcaatgaaa aaaactgtta
    49861 tttatactag tttatttgca gctttgtttt tagctgcttg tcaatcaaat accaatcaag
    49921 gcacatctgg gaatacgtct tccagtagcg aacagcgctc ttcacaggta tcggactcct
    49981 caacaaaagc aagcacatct tcgtctagcc agacaaaaag ttctagtgaa gcaagtactc
    50041 agtcagatat caaagtgtct gcagctcgtg cgatcgaatt gttccaagat aaatacccag
    50101 atactgcaat tactagtttg gaattagata gtgattgggg atcttacttc tacaagatag
    50161 aaggtgtcga tgatcaaaaa gaatacgaag caagaattga tgctcaaaat gaaaaaatgg
    50221 agacagagaa tccagaaact ctagatcgag atgaacaaaa tggtcaaaag aaaaaagaag
    50281 atggtctgga tgttgataaa ttgatcacga ttgaggaagc agcaaaaatc gccgtcgaca
    50341 aagtaggaag cggtacggca acagattggg atttggataa agatttaggt actacttatt
    50401 gggaagtcaa agtaaaagac ggcaatcaaa gtacatcagt caagatcaat agccaaacag
    50461 gcgaagtact gtcaactgaa caagatgatt aagaatttct tcggaaaaca agagttgatt
    50521 tttcttttag tcatgataca ctagacaaaa taacttgttt gaaggagcga tttacttgag
    50581 tttagaaacg acctttgcca atcatatctt tgccaatcct ttgatgaatg catctggtgt
    50641 ccactgcatg actacccagg aattagatga actggctcat tcagaagcag gagcatttat
    50701 cactaagagc tgtacgatca atgaacgaaa aggaaatccc gaaccacgct attttgatgt
    50761 tcctttaggg agtatcaatt ctatgggact tccaaactta ggtttttctt attatctaga
    50821 gtatgctctt gcgtatgaaa aagtgcagga aaaccaaaat caaccgttat ttttttccat
    50881 tgccgggatg agtgtccagg aaaacttgga aatgcttgag aaaatagaaa aaagtgggtt
    50941 taatgggatc actgaattaa atttatcttg tcccaatgtg cctggaaaac cacaactagc
    51001 ttacgatttt gaggccactt atgaaacact gaaagaagta ttcagcatct tttctaagcc
    51061 gctaggaatc aaattgcctc cttattttga ttttgctcat tttgatcaga tggctgatat
    51121 cttgaatcag tttccattga cctatgtcaa tgctatcaac agtgtgggca atggtttgta
    51181 tatcgataca gaacaagaag cagtagtcat caaaccaaaa gaagggttcg gcggcattgg
    51241 cggagagtat atcaagccga ctgctttggc gaatgttcgc gctttttata cacgtttgaa
    51301 accagaaatc caaatcattg gtacaggtgg gattcggaca ggacaagatg catttgaaca
    51361 tctgctatgt ggcgcaagca tgttacagat tggtaccgag cttcataaag aagggccgga
    51421 aattttttct agaatcataa aagaattaac gcagattatg tcagaaaaag gatatacgtc
    51481 aattgacgaa tttaaaggca aattacgcac catttcatag caaagagacg aaggtcatga
    51541 ttgatcttcg tcttttttta taaaaaacac tcgtctgttc attcctgtgc aagcgtactc
    51601 attgtatgtt agacgaactt gtgggaaaat aacagaggca aaaaggagga taatatgaat
    51661 gatgcaacag aattagcaaa agagcttaaa gaaaaagaat ttagttttca agaactagtt
    51721 acggatatac agaaaaaaat aaagaagcaa aatcatgaat tgaatgcgtt tgtcgcgctt
    51781 gaaccattcg agagtaattc ccacgagcta ttgattcccc agtcagaaaa agggccattg
    51841 gctggtatcc catttccttt aaaaatgctc ggtcaagaaa aaaagggatg gctagctact
    51901 tcaggatctc gtttatttga aacacaccga gcttctcata catctaatta tgtgcggcaa
    51961 gcagaagcga taggtctagt tccttttgga caaaccaatg cgccagagtt tggtttcaaa
    52021 aatattacag acccagtcat ctatggccca gcaagaaacc cttggaacct tgaatgtaca
    52081 ccgggcggat caagtggagg agctgctgct gcagtttcat caggaatcgt tcctttagct
    52141 ggtgctagtg atggcggtgg ttctattaga atacccgctt cgttttgcgg attgattggg
    52201 ctgaaacctt cgcgagggac gatgccagtt ggtccatatg cttggagagg atggcaagga
    52261 gctgctattg atttcggctt gacagtttct atgagagaca cggaggcttt attttatggg
    52321 atgagaagta tccattccgg tgcgccatat caagtatcgc cagttgagtg gcaaacacat
    52381 ccaagaaaaa aacggttgaa gatagctttt tgtgttgatt caccgatcca ctcacagatt
    52441 agtgaagagg cagtacttgc tgtaaaaaaa gctgtagatt tccttggaaa agaagggcat
    52501 gatttgacag agattcctta cccactagat ggtcgtaaac tgatcacgtc ttattatcag
    52561 atgaatggcg cagaaacagc agcaatgatc cgctcgattg aaacaggcat ccagcgagct
    52621 gtcagaaaag aagaacttga acccatttct tggactttgt tccaatatgg tcagaagctc
    52681 tctgccgcta cttacatcga ttctctgcat gtatgggatg aagcagcaat tgtcatggaa
    52741 aacctacatc aagaatacga tctattcctt tcaccaacga ctgcatttac cgcccctaaa
    52801 atcagcgaag atttgcaaag tgatgcgata agagcacaga tggaacacat cagtgattgt
    52861 tctgaagtag aaggtttaga cgttgtttct tccatgtttg aggagagctt gaagatcaca
    52921 ccttatacac agttggctaa tctgactgga caaccagcaa tcagcttgcc tactcatttg
    52981 acaaaagaaa acatgcctct aggaatccaa tttatggctg ccagaggaag agaagatttg
    53041 ctatttcaaa tcggctatct atttgaagca aatcatcagt tccatttacc aaatccatgg
    53101 aaatagcaga aacaaggaga agaagaatga agaaaaaaag attgtttgct ctaataacta
    53161 gtgccttttt tattttaagc gcttgcggta cagctgccgc taatcaggaa gataacagct
    53221 ggagtaatgt agaaaaaaag aaagaattag tagtagcgac ttctggcaca ctgtttcccg
    53281 catcttatta taatgatcaa aatgaattag ttggttatga tgtagatttg gcaaaagagg
    53341 tggctaaacg gttaaatgtc aaaatctcct ttaaagaata caacgtggat ggacaaatca
    53401 cttctgtttc aaaaagagaa tcagattttg ctgcgaacga ttttggtatc acaaaagaaa
    53461 gagaaaaaaa attctccctt tcagagactt tcaaatattc atttgacagt atgatcgtac
    53521 gaaaaaaaga tgattcagga atccactcac ttgaagatct taaagggaaa aaagcagctg
    53581 gcgaacccaa tactagctat atgcgtttag cggaaaaaca cggagctaaa ttagtcacat
    53641 atgacaatgc cacgaatgat cagtatttaa gcgatgtagc aaatggtcga acagatgtta
    53701 ttctaaatga ttattatctg cagaaaatgg ccgtatctgc tttaccagat atccctgtaa
    53761 aaatactgga gaacgtttat ttcaatgaaa acgaaagtgg ttttttattg aaagaaggaa
    53821 atgatgctct gaaaaagaaa atcgatggcg tattaaaaga gatgaaagaa gatgggacga
    53881 caaagaaaat atctgaaaaa tattttcaaa ctgatgtttc agtcaaaccg aaagaaacga
    53941 tggctgagtc ggtgagtgag tgatgtactt tcctctaatt gatttttccc tcatgtggac
    54001 ttcacttcct ttcgtactgc aaggcttggt atacacatta gggattggct tcgtgtcgtt
    54061 tcttttagga aatctggtag ggttgctttt gacagtttta ggtcttttgg attggctgcc
    54121 tctgaatgta tttattcgtt tttatttatc gttctttaga ggcattcccg cactagtcct
    54181 gctgtttctg ttatattttg ggttacctta tcaattaagt gccttaacgg cttcagtcat
    54241 ctgctttacg attaccagca gtgcatttat tggtgaaatc tatcgaggat cactagcagg
    54301 cgtttcttca ggtcagtggg acgctggttt agcttctggt ttttctttct ttggggtatt
    54361 gaaatatatc gttttgccgc aagcttttcg aatctcagta ccggcactca gtaacgtagc
    54421 tatggatcta gtgaaaggta cttcgttagc agcaacaatc acagtgccag aaatatttca
    54481 aaaagcaaag atcataggcg gtcggacatt cgattatttt tctctctata ttttagttgc
    54541 actgatttat tgggtcattt gtatcgggat cgaaaaagtg caacagtatt tagaaaaacg
    54601 ttctatgcat cagtcaatct gaaaaataga aatggtgcta gcgtggctgg caccatttct
    54661 attttttgtt tcttgtatat tctgcctagt attttaaaac catttcatgt aaaatgtaaa
    54721 tcgaaaatat gttcggaggt agaaaacatg aagaccccgc taaccatcga aatagaatcc
    54781 tcgttatacc aatattgcat cgagcaaggc ggcttggttg tagaagaagt cacgatgcct
    54841 gatgaccaag gaattgtaga tacgcttagc tgcttgacaa aatttgatgg aagccgagaa
    54901 tggcgatgct atgagctgaa agtgacaaaa gcagattttc gctctacagc caaactgtca
    54961 tttgttggac attataatta ttttgtttta ccaagtaaac tatatgaaga agtaaaaact
    55021 gaaatcccta caggaatagg tgttttagtg tatcgtccgt acacggttga agaggagatg
    55081 cctgcctctg gtacatttct gattgcgaaa aaagcagcaa agaaagagct tgctgtaccg
    55141 gaagaagctt tgacaaaccg cttcatggct tctttattca gggaagtaag aaaagcaaag
    55201 caaatggagc gaggcgtaaa atattttcca acagatcaat tgtacaaaga attgaaaaga
    55261 cgttcagaaa atcaagatcc attttctaac gaacgatatt atgaacggtt tttagaggaa
    55321 atagctagtc aggcaatcaa tgagttgcaa gaagaagtta tcgcgcttca gcatgattat
    55381 gaatatttga ggcaacaaca gcagatccgt cggttgccta cagagccatt ggagtgaaaa
    55441 atatgtacta tccttattta agaggaaaac aatttgacct attagcatta aaagaagcac
    55501 tgtcgcgagg attgctctcc aataaaatcc aaccagtgat cgaaccagta cgtgattccg
    55561 ctacgcttaa aaatgtgatc gaattatttc aaaaaaagca ccatcgaatc gctgtgatcc
    55621 aaaatcccca agtaggacaa ttcaaattat tcgatcagca tgtgcacagt tggacagtca
    55681 aagaaaatag ctctgtcgtc cctgcacaga tcttaacgcc agaaaatctc agtgaagtac
    55741 tggattcacc tccagcgttt cttgttttcg atggacagca ctacccaaaa gatacagagg
    55801 tgtggaaaca gttggcagga gttgattcga aatttttgat accagatact agtcgctttc
    55861 gcatctggct gcccgaaaac aaaattgtga tccgtgactc ttttcaaacg agaaagcatg
    55921 ttgaaagtta tgcagataaa acagatgact tttttagtga tgactatctt tttttccacg
    55981 cagacggata tattggattt tctgatttca cgatcgaagg tagccgttat ttcgataaag
    56041 ggttccctag ccgagcgctt gctatccatc taacgtatat tgatgcttac ggcaatatac
    56101 gcgtgaaaca ttttgtctct gacagtaacg actctgctaa agaccaagca ttgaagtttc
    56161 ttgaagcagg agataaaatg aaaaaatgga tcatgcgtca ccatcatcaa ctcttgatta
    56221 cttcaggaat gattgaatta ttggctcttt accagcaaca aaaattccct ggactgggag
    56281 tgttgaaaaa gtggtcactt atgcatcatt tggaacttat tagtcacctg ctagatcatc
    56341 caaaagattg gcttaaatat tattcaaaag ttcaggaact atatgaagtg atccaatgat
    56401 tagataatgt aaatgaaaaa ggaatagaaa agaggaaact ggatgtatat tcggcaaaca
    56461 aggaaagaag aagtagaaga agtggtagaa atcattaatg ccgcagcgga tttcattgct
    56521 tcgcaaggct ctcctcaatg gcaaaacggg cagaaaccaa caaaagaaac gattttgaac
    56581 gatattgaaa gaaaagaaag ctatgtatta atagtggaag atcaaattgc tggaacagct
    56641 gcattagtct caagcgtgga tcccgtttac acaaaaatag atggtagttg gaaagaatca
    56701 actggtcctt atttatcgat tcatcgtgtc gctttagcag aaagtactcg aggaaaaaaa
    56761 ctagcaaaac cgttcttaga acatttgttg acgattgcag ccagcaaaaa tatccaagat
    56821 atccgtatcg atacttatcc agctaatgaa ccgatggaaa agaccatcta ttctgctgga
    56881 ttcacttatc aagggatgat tgaatttcct tttgaacacg gcgaaagaaa agcatatcaa
    56941 ataatcatca attgactata aaatcatctg agcaaagata aagacattaa cactactgaa
    57001 aaaaacaatc atatgatagc caagaggttg taacagaagt ggccagcttc aaaaaacaag
    57061 aaagaatttc cgaaaatgct tctcaaattt ttgtggaatt ttagcgtatt ttacgaaata
    57121 gctgctttct gaaactccct tcatcgagag gtcgtgacat agcttttgtc acgacctctc
    57181 ggcttatcct acgagtatta ttcgatacat ttatatcaat aatccttttt ttattttatc
    57241 tgcagcttct tggaacgctc catcgtttga cacgactaaa agtgagggga tagctttcaa
    57301 acgtttcaac tgtgctaaat cttctacata aaccgccagt ctggctgaaa tctgttcaga
    57361 tgaatctcca cgttttttca ggcgcgattg caatgtttct ttagagatcg taataaagat
    57421 aggacggact accgtcccga aatactcata taaatgccaa aaaccatttg ctgtcaaaac
    57481 ggcgtaacaa tttcctactg tcagtttctt tttaatttct gctttactgg aaccatattt
    57541 atgttgagca tattggtccc actctgcaaa ctcatttccc ttgatcatct gattgaatgt
    57601 attgtcagaa acaaaataat aatcctcttg atctttttcc ccaggtcttg gtgatcgatt
    57661 cgtgtaagta atgatctttt gttccggctg aaaaatagct tctgcgagtg aagtttttcc
    57721 agaaccagaa ggaccgataa atacatagca ataattcatt gtcatttctc ctttgatgat
    57781 tgaacaagtg aaaaagcttt ggaaaatatt tccaaagctt ttaaaagtaa ctttgctttt
    57841 ttgtatatta taccagcaag ccagtcacaa tagcacttaa aaagctgact aaagtcgctg
    57901 ttagcaaaat tttcaatccg tgttttgcga caagatttcc tttttcttca tttaatccct
    57961 tcatcgcacc agaaatgatc ccgatagaag agaagttggc aaatgaaacg aggaagacag
    58021 atataattgc tgttgtacgc tcagtaaaat ggaaattacc atttgctaat tctgtcatag
    58081 caacaaattc atttgtaacc agtttagtcg ccatgatact accggcatcc actgcttctt
    58141 tccatgggat tccgctgatg aaggcaagag gggcaaagat gtaacctagg atctcttgga
    58201 aagtgatgcc gaatatcccg tggaagatag cattgatcat ggcaatcaaa gcaacaaaac
    58261 cgataagcat agcagcaacc gtgatcgcca catggaaacc atcaagaatg tattctccca
    58321 acatttggaa aaatgtctgt tctttgtttt cttcaatgat cagctcatct tctttttctt
    58381 ctaatacata gggatttaca attgaagcca gaatataacc gccaaataag ttcaaaacaa
    58441 gggcagtgat cacatattta gaatcgatca gtgccatata agatccgacg atcgacatag
    58501 aaactgtaga catagccgat acactcatgg tgaacaaacg tttctcagat aggaagggta
    58561 attccttttt tatggaaata aaaacttccg attgtcctaa aatagcagaa gctactccat
    58621 tataagattc caatttgccc atcccgttga ttttacttaa tcctagacca acagctttca
    58681 taaataatgg aagaatcttg atatagcgta gaataccgat aatagcagaa ataaatacaa
    58741 ttggcatcaa tacggaaaga aagaaaggtg cacttccttt atttgctaca ccggcaaaaa
    58801 caaagtcaac accttgccca gcaaaagcaa gaagatgatc aaacattttt gccaatacag
    58861 aaacaaccgc tgtaccaaaa gttgtacgta ataatacgaa accaagaaca agctgtaaaa
    58921 cgaacatcac aattagtggt ttcaatttga ttgctttttt gttactgctg atcaaaaaag
    58981 caagccctaa tgtgacaaat aaaccaataa tacctaataa ataactcaaa taatcactcc
    59041 tattccaaat tccgttttat tttacctgtt ttgcaaataa aatactaatc tttagtgaaa
    59101 ttttcattac ttttttagag tgaaacgcat tcattttcca ttagaaaata aagcgaacaa
    59161 aataaaaggt ttaaaaagtg taacgtctta gtaaagtcta taaaaaaacg aaactatcga
    59221 ttaagctaat agtttcgttt tcaacattta taagtaaact ttttctattg gtaatataga
    59281 ttttcggaag ggtagacagg tgcgcaggta atatcgattc ctagtttttt caatgtttgt
    59341 tcatcatttt tattgaccat cacagtagag tgggcttgtg ctccctcaag ttcagctagt
    59401 ttatcataag caacttgagc gattggattc gttactgcac tgattgataa agcgatcaag
    59461 acttcattga tgcttagtgc atccaaagag gaatgaagtg tctttgattt caatccttgt
    59521 attgtttcaa gaactaatgg tgacaataaa ggaatgtcat ctgcgatatg ggctaaatgt
    59581 tttactgcat ttaagataac tgccgctgac gcatccatca atgaacttgt acgcccggta
    59641 atgatgactt catctggtaa ttcgattgca atcacagcag aaggatcttt tgtaggactt
    59701 tgcgcttgaa tcttatctgc atagtcatgg gcagggacta ccacgtttcg gtcttcagga
    59761 cgtaaaccga cttcttccat aatcagtttg attcgattaa ctgcttcttc gtcgatcaat
    59821 ccttttttga agtcgcattc tgtttggaaa taacgacgga tgatttcttg tttagaggct
    59881 tcttggacaa cggtatcatc cacgatccca aatcctacac gattcacacc catgtcagta
    59941 ggagattgat aaacagattc tttaccagta attttttcaa taatacgttt gattacagga
    60001 aatgtctcaa catcccgatt atagttcaca gctactttat tgtaggcatc aaagtggaag
    60061 gaatcgatca tattgacatc ttttagatcg actgtggctg cttcataagc aatgttcagc
    60121 ggatgtttca acggaacatt ccatactggg aaagtttcga acttggagta accagctgca
    60181 tgtccttttt ggctttcatg atacagttgg ttcaaggaag tagcgagctt tccacttcct
    60241 gggccaggag ctgtcactac aacgattggt ttagttgttt caatatactc attttttcca
    60301 aaaccatctt ctgagacgat tttttctacg ttgactggat agtcttcgat ttctgagtgt
    60361 ttatagacct tgatgtttcg tctttctagt ttattgataa agactttcgt ggcaggctgt
    60421 ccactatagc gagtgataac gacactgttg attaataagc cataaccacg taattcatcg
    60481 attaaccgca agatatccat atcataagta atcccataat caccacggat cttattacgt
    60541 tcaatatctc cagcatagac gcaaatcagt atttcagctt gatctttcaa tttttgcagc
    60601 aatttgattt tcgcatcttc ttcaaatcct ggaagtacac gtttcgcatg cttgtcgtca
    60661 actagcttcc caccaaattc taaatataat ttgtcatagt ggttcactcg ttccaaaata
    60721 tacgctgact gctcttcaat atatttttct gaatcaaaac caattttttt cactaggttc
    60781 cctccttaag tactaaagca ttttttcatt tttttgagga ttcgtcaact ggaagagcga
    60841 aattcctcaa aaaaacaaag aaaatggttt aaaccaactt gtctagatag ttattcattg
    60901 gtatgatagt ttttgataag gaaggtgcta catgaaaaaa agacagaaaa agaaaaacgc
    60961 atataaacac tatatccgtt cgatttttac cggttatgaa agaatgcttg aagatcccga
    61021 attggaacaa ttgacgttta cttatctaaa cgaagaaacg cagctaactc gtgatgagca
    61081 tcagcgcatt catttcacaa caagagatct gcctagtaaa taaaaaataa gataaaggtc
    61141 atcggattcg attctgtcct atcagatgga aagactagtt gatcgaaaaa aaggctgtac
    61201 caaattttgg tgcagcctgt tttatggagc attgcccgct cttttttata acaatttttt
    61261 acagaaaatg gtactattga agtattaata agtgaaaaac agatgagtaa gaggaggcaa
    61321 gtagtatgaa agagccagat acaaatgcaa attatgagcc agacgaacta gaggagacga
    61381 atgcggatca ggatacagga caagaatcgt cttcttttta cgatatcaaa gaaaaagcaa
    61441 gcatgaatct ttcccaatta ttagaagact tacaggcttt tgaaaaagct gtccgcgaag
    61501 aagatatccc tgagatctac cgtttataca atggccgatt gaatgaagaa ttaaaaagaa
    61561 gctcaaatga aaatcacgag atcgatcaat tgctgatgcg taagatccat gatcgttttt
    61621 tacaggaatt tccttttatg gaacaagtag aaaacatctc gccaacgatg agctattaca
    61681 aaatcggcac ttattaccat gatcggccca cggtcggtat tgatgcaagt ctgccagaaa
    61741 tttttgtttt gcctaagatc gatgaagagt gggagaaata ttcccaaccg gagtcaacgg
    61801 actatgacaa aaaaatcaat gagctagacg caaaggtgat cactgctcag acagaaattg
    61861 aacgtttaga tgaacaaata aaagaaatca atcgacaaat gactgatttg aacgacaata
    61921 aaggattctt gaatcgtaaa aaaatcgagg aagaaattca agaattagag aagaagaagc
    61981 aagtactatc taatgaaaaa ttaggctggc ttccttatat tgagacgcct gaaacaatcc
    62041 agcagcaaaa agaagcattg aagcaagaag caagagcaga ccagcttcgt gcagccatcg
    62101 tagaaaaaga acaaaggcaa atcagacgct atttcggtag caaagaggga ttcggtcaag
    62161 caatccatga attcttgatg aattatcttg gcaatgagaa tcctaataat caatctaatg
    62221 aaggaggttc cgagtatgaa tgaacacgat gaagcagaat ggacaaaaaa tgaagaacaa
    62281 acagaagatc atgaagaaac gaacacttca ttggaagaac aaacaagtaa tcctctggat
    62341 gacacagaag aaatccctat gctagaggaa acaaaaatac acgaaaaaga aaaagtaact
    62401 ccgacgaata cgaatactgc agaaaacacc catgcttacg aaataactga aaatcccggg
    62461 tatttagaag gaaaagagca agatcatcaa gatatcgtca gtttgattga aacagcggaa
    62521 gcagaattag cggatttacg tatgagaaaa gatttgatcg aatcagattt tccacaatta
    62581 ttagaatcat atgatcgttt tatattgaat gaagaaccaa tctccacgat cggaaagaca
    62641 agcctgatcg aatacttcac gtatgaatta ctgaaaccgt tgaatgatat tggtctggaa
    62701 cggtcaaatt cagcttatga cacatggaaa acaagacatt tttcgatcaa ttatacgttg
    62761 aatggacaaa acgaattagt cttttccttt gtattaccga ccatcgatca acgataccaa
    62821 gtggaatcga tgagtctcct aaacgtatca ccagagacaa tggaaatcaa tgtccaagat
    62881 gaccgggtgc tttctttgat ccgacactgg tcagtggatc gagtattctc tgcagggcag
    62941 atcacgatat tcaatcacaa attgaatcaa ttattagccc atgcaagaac acttggtttt
    63001 accgttaatc agacattact agacaatacg aagccacttc gtttgaatct tcagagcgaa
    63061 tttgaattga cgaatgaagt attggatgat atttttatcg tggcaatgaa aaatccatcc
    63121 tacgatatgg aaaagatcaa cgaaactact tatcaagtac gattagataa aggacagtct
    63181 ttaacgataa caacaaacga aaaagatcaa acagtcttgt cgatctcatc tggagactac
    63241 ccacgatctg tcattgattt ctttatcaat tacgaattct tggtcccatt aatggtgaga
    63301 aagtcctgat tggataagtt ggattaccag atgtcattca gataattctg ttccaaagga
    63361 cttgcggcag ttatccagaa taattgtcac aagtcttttc tgtatgtact aaatggacgt
    63421 gaataacttg ctgatgaagg agggatttca ttaggaatgt ccacaaataa aatagcacag
    63481 cttagcttat tgtctgctgc ttgtgtagca ggtaggatcg cttttcaatt tattccgaat
    63541 ttccagccaa tgactgctat ttttttattt atagtacttt acttaagctt aaaagatgcg
    63601 ttaattgtca tgagtctttc tattgcgatc agcagttttt attttggtgt tggaccatgg
    63661 gtcatcggcc aatggatcag ctatactgtt gttctgagtt tgttttcaat cgtctgcttg
    63721 agaaaaacag tgcaaaggaa tctatggtta aagggagtct gtttttttct ggcgggcatg
    63781 gtttacggaa taggtatgac cgtttttgat acgctgttat atcgtttgcc tcaaccttgg
    63841 atctattacc ttcaaggggt gtcgtttgat ttgatgcaca gtataggcaa tgttgtgttc
    63901 tttcttgttt ttctgccagt cgtcaaacgt ttttacaatc attcaggagg aaaaaatgaa
    63961 aaaaataatt tatagtttgc ttgcaatgag tgtcagtata ctcattttga caagttgcag
    64021 tacaacctat acacaatcgg cttccaacca aaaggaggaa gtaaaaacat cgcaaaaagc
    64081 aacaatcacg ttacaagaaa atggtcaaaa cttttcggaa aaagaagtgt tcttcaaaaa
    64141 aggcgatgat ttactgagta ttttgaaacg aaattttgaa gtcaaagaag ataacggctt
    64201 catcacttcg cttgaagggc actcacagga tgagaaaaat caactctatt ggatgtttac
    64261 tgtggacggg aaaagtgcta ctactggtgc caaagagatc aaattgaatg atggacaatc
    64321 ggtcattttc aatttgtcta aactataagt ttcataaaaa actgttaaag ggcgaatatt
    64381 gccataattc agtgagttca gttgatgaaa gaaggaaaat aacatggaaa attttaaaaa
    64441 aagcggaatg ttttccgtaa ttgctgctct aggggctaat attctagttg ctatcagcaa
    64501 atttgtcggc tatgcaatca gtggcagtgc cgccatgcta aacgaaagca tccacagtat
    64561 cgttgattgc agcaatcaga ttttgctgtt attcggtgat aaacaagcca ataaaggaca
    64621 gagcgagttg catcaatttg gtgaaggacg agcaaaatac tttttcagta caatcgtcgc
    64681 aatgatgctt ttcttcggcg gtggggcact tggggtgatg gaagcttttg aaaagttgac
    64741 gcatccatcc catgaagtgg gtaatccgat cattgttata gcaatactgt tatttggttt
    64801 ggtaatcgaa ggaagttcgt tacgcgtggc aatgaaagaa atcaaagaat tgaatactga
    64861 aaaactttcc acgatgcgct ttttgcgcga aagtcgacac agtgaaatac tgattatttt
    64921 tacggaagac ttgtgtgctg taatcggttt ggtcttggct ttagcaggaa caatattgac
    64981 ttatgcaacc gggattgctg catttgatgc gatcagcgga ttgctgatcg gtttcttgct
    65041 gatgggagcc gccattttcc tagcaaaaga attttatagt ctgatcatcg gtgaaagcgt
    65101 gacagcaagc gatttatcaa aaatcaagtt agcttttgaa cgtcctgaga tcagtcggct
    65161 gatcgatata aaaacagttc atttaagtcc aacggagatt ttagttgcag caaaagcaga
    65221 tatcgttggt aacaaagaga atgaagctta ctcggtcatc aatgatattg aaaaaaatat
    65281 gcgtcagtct cttcctgaca agaagatgta tatatacatt gaaacagata aatatgaccc
    65341 aaattattct cgtaaataaa acgtttattc atgaattgaa gcaaaaatga gaaaaatata
    65401 atcttgtttt taaataaaac ggctacaatt tagcgaattt atgttacaat gagtccgctg
    65461 tttatttgtt gtaattaaag gagatatttt aatgaacaaa aagaaaagtg cgccatggct
    65521 tcaagctttg catgcagtga tgccactttg tataagctat ataccagtag gattagcttg
    65581 cggagtattg ttgcaaaaag ttggatttga tcctctctta tcaggactgt tatcgattct
    65641 ggtcttttcc ggtggcgcgc agtttttagt tgcatctatg cttacgacgc aagcatcatt
    65701 tgcaacgaca ttattgatgg ttttcttttt agaattacgc tatacattat taggagcaag
    65761 tcttgccggt tttatgaaaa aagaaaagcg gacgtttttg gctgtctttt ctcaatcact
    65821 gaatgatgaa aactatgcgg tgaactactt aaagttttca actgaccctt cctggaataa
    65881 aagaaaggct ttatatgtca attggttctc aatgactgca tgggccggta gcaatatgat
    65941 cggtaatttt ttcggctcgg tgattcgagt agatgctgac cttgtccatt ttgctttgac
    66001 ggccatgttc attttcatgt ttatcatgca gatgaaaagc gcactactga tctttacagg
    66061 tttattctca ggcgtattag gggtcgtatt catggtatta ttccaaaata ctttcggatt
    66121 gattgcagca acagtcattg cttcatttgc cggatttatg ctggagaagg cattcaaaaa
    66181 agaaaaagct aagaaactaa gagaccgcgg taaagatgtc aatgagccgc tcttcaactt
    66241 cccggaacag gaggccaatc accatgacta aactatattt gctactttta gttgcttgtc
    66301 tgttcatcgt ggcatatatc ccgcgcttgt tcccgatgct ttattttact catcgaaaag
    66361 tgccatcctg gttcagtgac tggatgaaat atgtaccggt ggcattattt gcggcgttag
    66421 cttttaaaga tgttttcatt actcatgaac atctggatat tgcttggaat atcaaaatag
    66481 ctgcaatgat tctagtagca ggcgtggctt acaaaacacg ttcgatggcg ttgtcagttt
    66541 tgacaggact tgcgtctgtc tttttgctgt ctatgcttta aactgtttta ttttattaaa
    66601 aacagagatt gcgacataag ttgttcaaac tgatctatcc gaactgcatt tgcaacagtc
    66661 aattctggat gcaagtttat tcgtagggat aaaaaagatt agtgttggct tatgatgcaa
    66721 gctctttttt attttaggaa ttagagaggg atttgtcctt ctatgatttt ttgctataat
    66781 agggaagatg tttattttat gaaagaatag ttgagggaat ttaaatgaat aaaaaattga
    66841 tgcaaggaac attttggcta acctttgcta atcttctatg taaagtttta ggggtagtct
    66901 atttgattcc ctggctaagt atgatgggga acaatcaaga tggtatgctt gctacgacat
    66961 tgtataatgt tggctatctt ccttatggat tgttcctgat gcttggtaca gtcggatttc
    67021 caaatgcgat tgcaaaaaaa gtagctgtag ccacaaaaga aggagataac gctgcttgtc
    67081 ggctgatttt tagaagtacg atcaatatca tgtttgtcat cggtatcgcc tctgctgcac
    67141 tgatgtattt atttgctcct ttactagccg gaatcagtcc gattgctaac gttaataatg
    67201 ggatactagc tatcagaagt ttatgcccat ctttgattgc tatcccaatt cttagtgcaa
    67261 tgagaggata tttccaagga aaaaatgatc tgcggcctta tggaacttct ttgattatcg
    67321 aacaggctgt gcgagtgatc gttatcttgg ctggtactta ttacttgcgc gtattgactg
    67381 acggaaccat attagaagct gtattgatca gtacagtcgc ttctttcttc ggtgggttag
    67441 ctgcgattat ccatatgtat atcgtcggac gcagaaaaga cttcttcgaa cttaaagatt
    67501 ttttgatttc cagccgttat ttcgaaagag aaaaccgatc tgcctctgtc tctatcattc
    67561 gggaaacact accgtttatt tttgtcggct cagtcattac tatcgttcaa atgatcgacc
    67621 aagtaacgat gaaaccactg cttcactttt tccgtccaga gatcgcagat cagcaactgg
    67681 aattcttatt cagccgtgct tctgtcaatc caaataaatt gacattgatc ctgatctcca
    67741 tggtaggaac agtagctata agcagcttgc caatattaag cacgatgaag aaaaaagatc
    67801 ggatacaaat cgaaaaaaca gtaggagata gtttttcgat tgcgttattg attcttcttc
    67861 cttcattgac tggaatgtct ttattagctg gaccactata cacattgttt tttggctatg
    67921 atcctgaaag tgtcggatat ttccaaatgg ctttgctagc ttctttgttt ttctctttat
    67981 tcacgatcct atctacgatg cttcaatctt tgaatcacca tcgttttgcg atccgattaa
    68041 ctgtagaagc aattatcctg aaagtcatct tccaagtaat cggattagga ttagttggcg
    68101 gatacgggat gagcttatca aatggcttag cattcggtat tgtctttgtt cgcggctatc
    68161 actttatgtg caaagaatac cgaatagcac ctttagcaaa gatcagcaat tttttcttaa
    68221 aaactttccg ctccaccctg attatgcttg cagtatgttt tgccgttttc tttttgctga
    68281 atcagcgatt gtcaatggaa tcaaaaagct atgcaatgat ttattgtgtg gctgtaggaa
    68341 gtattggcgg cttagtattt ttattttctc aattcggaaa aaatgggttt cgaatgttga
    68401 aaaatgttaa acatcatagc agaagcaaag aataggttaa ctaatcgttt tttgctgtca
    68461 cttgaaaaac ggcaactcgt atgattacgc ttgtcgtttt tctattttcc aattttagtt
    68521 tcatatctaa tcttacttat gatatagtta taaggtctgg aaaaattctt tgaaacgagg
    68581 aaattaaaca catgaaactt gcagtagtta cagatagttc ggcatatctg ccggaacgta
    68641 tcaagaacca tcccgactta ttcgttattc caatcccttt gattatggat gggaaaattt
    68701 ataacgaagg aatcgatatc gaagcggatg aatattatgg acttttaaaa aacagtaaag
    68761 attttcctac tacttcacaa cctgtcgtag gggaagtttt gggtttatat gaggaattga
    68821 aagagaaagg gtatgacacg attttaagta tccatctttc ttcagggatt tctggctttg
    68881 tcaacacatt atttgcgatg aaagatgaca tcagcggagt aacggtcatt ccttatgact
    68941 ctaagatcac tagtatgccg atgggacaca tggtagaagc tgccttagac ttaaatgatg
    69001 aaggtcagtc ccttgatgaa atacttgcac atatcgatcg aatccgagat aatacctacg
    69061 cttatctgat tgtagatgat ttgaacaatt tagtacgagg aggacgtttg acaaatggtg
    69121 cagcgctgat tggcagctta ttaaaaatca aaccgatcct gacattttct gaagggaaaa
    69181 ttgttttatt cgaaaaaatc cgttcaagta aaaaagcatt tgctcgagct gaagaaatta
    69241 ttggtagaag aaataacgaa atacaaccgc cagtcaaact ttacgtgata catgcaaata
    69301 atttagaagt agcgattgcg gagaaagaga aattgcagaa aaaatatcca aatgcagaaa
    69361 tcgaaattgg tcatttcgga ccagtcattg gtacacattt aggtgaaaaa gccatcggca
    69421 ttgccatttc agcgcaataa ttccttttat tcaaaaaaca taccaaaaat attccaacat
    69481 catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg gtgtttttat
    69541 ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat aatgtcgcat
    69601 atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa aaggatgagg
    69661 agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg ttgtagaaga
    69721 ctatgataaa gttgatggaa gaaatgcatt aaatacggat gcaaaagggc tttcactggg
    69781 ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat ggcgacatac
    69841 tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt tagatttagc
    69901 gatcttagct gcacaaggaa gtttatactt ccaagatgct tatcgttatg aaaaatttta
    69961 tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa tgacggtaga
    70021 agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg atacactaca
    70081 aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt tagatgagct
    70141 aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa tttctcacta
    70201 acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa accgaaaagc
    70261 catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa ttggcaagat
    70321 cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg gaagacacaa
    70381 ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac ctgttttgca
    70441 gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc taataaggaa
    70501 caagaccaag aattactaga agctatttac caaacagata cacttgaagc tgtactgaca
    70561 ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt agcagagcta
    70621 gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta cccttctgaa
    70681 atcgttcgag cgattgaaaa aggatattta ttagagattc aagagccgac tgtcatccga
    70741 gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat gttgaattta
    70801 ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac aaatcgaaat
    70861 taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca tgcggaaaaa
    70921 atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac tatgatccaa
    70981 aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga aactgcaaaa
    71041 gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc aaatacctat
    71101 aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa attatccact
    71161 gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt gctactggaa
    71221 cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca tacaaaagga
    71281 agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc tgaagaaatt
    71341 ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg agaagagaaa
    71401 caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga acgcagtagc
    71461 gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca agaaatgtct
    71521 gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa agaagcgcga
    71581 caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg accagctttc
    71641 caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat agtagaatca
    71701 cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta ccaaaaagga
    71761 aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt gagaaatttt
    71821 gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg gatcgatgaa
    71881 tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc gattgccatt
    71941 gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac agcagaccgt
    72001 tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg atttcaatgg
    72061 ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg tgctgcactt
    72121 cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt gcttaatatt
    72181 agtgacggtc agccaaaagc gttgccggat tatacaggca aaaaagcaaa agaagatatc
    72241 cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc agctattggc
    72301 caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga tattactgac
    72361 ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg ataaaataaa
    72421 gctcaacgaa cgcacttagc agattcgttg agcttttcgt ttctagttac cggtaacaag
    72481 gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag tgaaacgata
    72541 tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat cctgctagtt
    72601 tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa aaacttaatt
    72661 atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa tctccatttg
    72721 tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc ggaacgatgg
    72781 aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg attatggatc
    72841 ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa aaatctaaag
    72901 aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa gaaccgaata
    72961 attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact ggacagtatt
    73021 atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag aaattgaaag
    73081 aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga tttcgtttag
    73141 atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag aaccctttct
    73201 caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa catgtttttg
    73261 aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa gaaggaccac
    73321 aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat atccatatct
    73381 ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacttgca ttaagcgctt
    73441 ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac catgatgttc
    73501 ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg tctgcaaaag
    73561 caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa ggacaggaaa
    73621 tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta gattcgaaaa
    73681 gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg gatatcattc
    73741 gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa caatatgctg
    73801 gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg atcaatgtag
    73861 aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg atccgcatta
    73921 gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat gaacatccac
    73981 aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg gtaaacttaa
    74041 cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg acattactct
    74101 tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct tatgaagctc
    74161 ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg gactttgcga
    74221 ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct aggaccaccg
    74281 atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt tttcaaacga
    74341 ggtctgacag gtacttggac atgtttgata tgcataccga ttgatgtgtc accgatatcg
    74401 ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg gtcaaaagca
    74461 gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat ttcgaaattt
    74521 tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc acaaccttgg
    74581 acagctagcg caatttcttt aggatccaac aactctttta acgtacggat gatccattga
    74641 ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct tgtgctgcat
    74701 cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc attaactatt
    74761 tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa ccttcttttt
    74821 caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag gaaaggcaag
    74881 tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc cagccggcca
    74941 atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc ttcctaataa
    75001 aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa catatccttg
    75061 taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa tcaaatcgat
    75121 caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa gaaaagcagc
    75181 ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta cagggatcaa
    75241 aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg ttaattgctt
    75301 agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt ccggtatgat
    75361 cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt tctgctactg
    75421 ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa aaggcacaac
    75481 cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca taatgatcaa
    75541 ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct ttgatcacaa
    75601 tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct gttaacgaag
    75661 agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta atcatcgtaa
    75721 caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc acagcttcag
    75781 tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt gtttttatga
    75841 agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg ataccatcta
    75901 atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa gatatagatt
    75961 ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg cttagtccga
    76021 atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca gcaaatggat
    76081 cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat aagagtataa
    76141 cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc caatttaaaa
    76201 aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt taggactttt
    76261 gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct gttcgcggga
    76321 ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg acttgctccc
    76381 accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt tgtggagaat
    76441 gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct aataaaaagt
    76501 cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga tctttcaaaa
    76561 ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa tcgccataaa
    76621 cattttcctg gaaccgtgca tcttccttgt aataagtcaa agcccgtcct ttagacagta
    76681 attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct gtaatccgag
    76741 cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt ttcaaactct
    76801 ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat acttgaagaa
    76861 ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg ttgtttatag
    76921 catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc tccttagcag
    76981 taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc cggtgagctt
    77041 ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata ggaaatccca
    77101 tggacatcaa agaagagata ccaatattga atctggaatg cactggagag atgtgaaaag
    77161 cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc gtccgccatc
    77221 ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca gaagtatggg
    77281 tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa gcaggttgtt
    77341 ccattaaata aagaatagg
//
LOCUS       DACEPD010000002        71525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.002, whole genome shotgun sequence.
ACCESSION   DACEPD010000002 DACEPD010000000
VERSION     DACEPD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.002"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..718
                     /locus_tag="IW797_000078"
     CDS             <1..718
                     /locus_tag="IW797_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ1222884.1"
                     /translation="EVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSI
                     FGYLAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKL
                     DDGKFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVM
                     INLDGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERL
                     TDEDIDGDGKIADETPNQKN"
     gene            829..2202
                     /gene="rlmD"
                     /locus_tag="IW797_000079"
     CDS             829..2202
                     /gene="rlmD"
                     /locus_tag="IW797_000079"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1222885.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            2205..2333
                     /locus_tag="IW797_000080"
     CDS             2205..2333
                     /locus_tag="IW797_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1222886.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            2773..2961
                     /locus_tag="IW797_000081"
     CDS             2773..2961
                     /locus_tag="IW797_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ1222887.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            3039..4052
                     /locus_tag="IW797_000082"
     CDS             3039..4052
                     /locus_tag="IW797_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1222888.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(4119..5153)
                     /locus_tag="IW797_000083"
     CDS             complement(4119..5153)
                     /locus_tag="IW797_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1222889.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            5268..7493
                     /locus_tag="IW797_000084"
     CDS             5268..7493
                     /locus_tag="IW797_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ1222890.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            7511..9394
                     /locus_tag="IW797_000085"
     CDS             7511..9394
                     /locus_tag="IW797_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ1222891.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            9375..10349
                     /locus_tag="IW797_000086"
     CDS             9375..10349
                     /locus_tag="IW797_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ1222892.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            11008..12030
                     /gene="aroF"
                     /locus_tag="IW797_000087"
     CDS             11008..12030
                     /gene="aroF"
                     /locus_tag="IW797_000087"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ1222893.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            12063..13127
                     /gene="aroB"
                     /locus_tag="IW797_000088"
     CDS             12063..13127
                     /gene="aroB"
                     /locus_tag="IW797_000088"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ1222894.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            13129..14295
                     /gene="aroC"
                     /locus_tag="IW797_000089"
     CDS             13129..14295
                     /gene="aroC"
                     /locus_tag="IW797_000089"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ1222895.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            14312..15403
                     /locus_tag="IW797_000090"
     CDS             14312..15403
                     /locus_tag="IW797_000090"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ1222896.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            15416..16711
                     /gene="aroA"
                     /locus_tag="IW797_000091"
     CDS             15416..16711
                     /gene="aroA"
                     /locus_tag="IW797_000091"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ1222897.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            16704..17213
                     /locus_tag="IW797_000092"
     CDS             16704..17213
                     /locus_tag="IW797_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ1222898.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            17210..18046
                     /gene="pheA"
                     /locus_tag="IW797_000093"
     CDS             17210..18046
                     /gene="pheA"
                     /locus_tag="IW797_000093"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ1222899.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(18109..19845)
                     /locus_tag="IW797_000094"
     CDS             complement(18109..19845)
                     /locus_tag="IW797_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1222900.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(19842..20771)
                     /locus_tag="IW797_000095"
     CDS             complement(19842..20771)
                     /locus_tag="IW797_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1222901.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            complement(20771..21349)
                     /locus_tag="IW797_000096"
     CDS             complement(20771..21349)
                     /locus_tag="IW797_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1222902.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            21622..22908
                     /locus_tag="IW797_000097"
     CDS             21622..22908
                     /locus_tag="IW797_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1222903.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            23045..23914
                     /locus_tag="IW797_000098"
     CDS             23045..23914
                     /locus_tag="IW797_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1222904.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            23911..24798
                     /locus_tag="IW797_000099"
     CDS             23911..24798
                     /locus_tag="IW797_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1222905.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            24965..25648
                     /locus_tag="IW797_000100"
     CDS             24965..25648
                     /locus_tag="IW797_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1222906.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            25645..26418
                     /locus_tag="IW797_000101"
     CDS             25645..26418
                     /locus_tag="IW797_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ1222907.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            26415..26813
                     /locus_tag="IW797_000102"
     CDS             26415..26813
                     /locus_tag="IW797_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ1222908.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            26998..27669
                     /locus_tag="IW797_000103"
     CDS             26998..27669
                     /locus_tag="IW797_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1222909.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            27728..28168
                     /locus_tag="IW797_000104"
     CDS             27728..28168
                     /locus_tag="IW797_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222910.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            28180..28848
                     /locus_tag="IW797_000105"
     CDS             28180..28848
                     /locus_tag="IW797_000105"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1222911.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            28912..29535
                     /locus_tag="IW797_000106"
     CDS             28912..29535
                     /locus_tag="IW797_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ1222912.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            29591..29977
                     /locus_tag="IW797_000107"
     CDS             29591..29977
                     /locus_tag="IW797_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ1222913.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            30190..31581
                     /locus_tag="IW797_000108"
     CDS             30190..31581
                     /locus_tag="IW797_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ1222914.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            31928..32575
                     /locus_tag="IW797_000109"
     CDS             31928..32575
                     /locus_tag="IW797_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1222915.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            32968..35613
                     /locus_tag="IW797_000110"
     CDS             32968..35613
                     /locus_tag="IW797_000110"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ1222916.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            35923..37236
                     /locus_tag="IW797_000111"
     CDS             35923..37236
                     /locus_tag="IW797_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ1222917.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSISDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            37226..37879
                     /locus_tag="IW797_000112"
     CDS             37226..37879
                     /locus_tag="IW797_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1222918.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            37934..38605
                     /gene="radC"
                     /locus_tag="IW797_000113"
     CDS             37934..38605
                     /gene="radC"
                     /locus_tag="IW797_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAQ1222919.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(38500..39852)
                     /locus_tag="IW797_000114"
     CDS             complement(38500..39852)
                     /locus_tag="IW797_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1222920.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(39918..41207)
                     /locus_tag="IW797_000115"
     CDS             complement(39918..41207)
                     /locus_tag="IW797_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1222921.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(41294..41998)
                     /locus_tag="IW797_000116"
     CDS             complement(41294..41998)
                     /locus_tag="IW797_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAQ1222922.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            42198..42464
                     /locus_tag="IW797_000117"
     CDS             42198..42464
                     /locus_tag="IW797_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1222923.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            complement(42632..42862)
                     /locus_tag="IW797_000118"
     CDS             complement(42632..42862)
                     /locus_tag="IW797_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1222924.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            42934..43089
                     /locus_tag="IW797_000119"
     CDS             42934..43089
                     /locus_tag="IW797_000119"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222925.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            complement(43398..43622)
                     /locus_tag="IW797_000120"
     CDS             complement(43398..43622)
                     /locus_tag="IW797_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1222926.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSPGNDPHPPKG"
     gene            43889..44224
                     /locus_tag="IW797_000121"
     CDS             43889..44224
                     /locus_tag="IW797_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222927.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            44457..45398
                     /locus_tag="IW797_000122"
     CDS             44457..45398
                     /locus_tag="IW797_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ1222928.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEKGIK"
     gene            45400..46290
                     /locus_tag="IW797_000123"
     CDS             45400..46290
                     /locus_tag="IW797_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAQ1222929.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            46333..47133
                     /locus_tag="IW797_000124"
     CDS             46333..47133
                     /locus_tag="IW797_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ1222930.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            47148..47999
                     /locus_tag="IW797_000125"
     CDS             47148..47999
                     /locus_tag="IW797_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1222931.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            47996..48355
                     /locus_tag="IW797_000126"
     CDS             47996..48355
                     /locus_tag="IW797_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ1222932.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            48370..48531
                     /locus_tag="IW797_000127"
     CDS             48370..48531
                     /locus_tag="IW797_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1222933.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            48528..48854
                     /locus_tag="IW797_000128"
     CDS             48528..48854
                     /locus_tag="IW797_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222934.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            48851..49666
                     /locus_tag="IW797_000129"
     CDS             48851..49666
                     /locus_tag="IW797_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAQ1222935.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            49805..50116
                     /locus_tag="IW797_000130"
     CDS             49805..50116
                     /locus_tag="IW797_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222936.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            50113..50343
                     /locus_tag="IW797_000131"
     CDS             50113..50343
                     /locus_tag="IW797_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222937.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            50350..50553
                     /locus_tag="IW797_000132"
     CDS             50350..50553
                     /locus_tag="IW797_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAQ1222938.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            50550..50849
                     /locus_tag="IW797_000133"
     CDS             50550..50849
                     /locus_tag="IW797_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222939.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVINNEV"
     gene            51092..51505
                     /locus_tag="IW797_000134"
     CDS             51092..51505
                     /locus_tag="IW797_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1222940.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILVALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            51518..51904
                     /locus_tag="IW797_000135"
     CDS             51518..51904
                     /locus_tag="IW797_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222941.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            52455..52679
                     /locus_tag="IW797_000136"
     CDS             52455..52679
                     /locus_tag="IW797_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222942.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            52697..53125
                     /locus_tag="IW797_000137"
     CDS             52697..53125
                     /locus_tag="IW797_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222943.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            53103..54521
                     /gene="terL"
                     /locus_tag="IW797_000138"
     CDS             53103..54521
                     /gene="terL"
                     /locus_tag="IW797_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HAQ1222944.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            54533..56068
                     /locus_tag="IW797_000139"
     CDS             54533..56068
                     /locus_tag="IW797_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1222945.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            56148..57026
                     /locus_tag="IW797_000140"
     CDS             56148..57026
                     /locus_tag="IW797_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ1222946.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            57028..57345
                     /locus_tag="IW797_000141"
     CDS             57028..57345
                     /locus_tag="IW797_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222947.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            57394..58095
                     /locus_tag="IW797_000142"
     CDS             57394..58095
                     /locus_tag="IW797_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ1222948.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMIMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            58110..59000
                     /locus_tag="IW797_000143"
     CDS             58110..59000
                     /locus_tag="IW797_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAQ1222949.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            59023..59238
                     /locus_tag="IW797_000144"
     CDS             59023..59238
                     /locus_tag="IW797_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222950.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            59250..59591
                     /locus_tag="IW797_000145"
     CDS             59250..59591
                     /locus_tag="IW797_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222951.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            59591..59962
                     /locus_tag="IW797_000146"
     CDS             59591..59962
                     /locus_tag="IW797_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222952.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            59955..60350
                     /locus_tag="IW797_000147"
     CDS             59955..60350
                     /locus_tag="IW797_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1222953.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            60352..60729
                     /locus_tag="IW797_000148"
     CDS             60352..60729
                     /locus_tag="IW797_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222954.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            60730..61338
                     /locus_tag="IW797_000149"
     CDS             60730..61338
                     /locus_tag="IW797_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1222955.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            61338..61679
                     /locus_tag="IW797_000150"
     CDS             61338..61679
                     /locus_tag="IW797_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222956.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            61921..64710
                     /locus_tag="IW797_000151"
     CDS             61921..64710
                     /locus_tag="IW797_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAQ1222957.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVESVK
                     DAWSGIKEWFSNTWQGIKNGATGLFDKTVETSMNAVDSVKNAWSNTKQWFSDIWQSIK
                     DSATEKWNEIKGSIMEVAGPLITGLKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LTPVLFITSMISGGWEETKNNMIGVWNNIKESALNIWESIKNIFVSYITNIYFAALNI
                     WTGFKLTLINIWNEVVSQAKSIWINVKYFFINLWIDIKYFAIQKWIELKFGIIQTWID
                     LKYNAITTWNNIKQFFKDTWQNIKDIAYNTWISIKNSMINTWNNIKESFWNIVTGIVN
                     SAENAWTNLKNGVSKAINRVKEIFDSLREINLFEIGKNIIDGLINGVVEKWNALKKTI
                     KGIAGSIKDSIKGALGIHSPSRWMRDMVGKNIVQGIIVGIDKEKSKLDQTMTDLVKTP
                     SVQPVITGSNSQPVVQAKQNTSSNAVNEIHLHLNVYGDLPDSMIKQIAKKMKTELTRQ
                     MKRDADAVGGTLYAT"
     gene            64700..65440
                     /locus_tag="IW797_000152"
     CDS             64700..65440
                     /locus_tag="IW797_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1222958.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKAYYKNVTRKIECYYKAPSIDLVQEWEDRITEWLDMNSYSDFILYYDQQYIYQ
                     AIVTEAPEFKGTRKTGNIVPFEFTVSLRPFKENYSGRFAIQQIKAFELFNPEKYASKP
                     LIKLSGSGDASFYINNDKYDLKLLDRELYIDSKLEESYRKLDDNLEHQDHVTLFLDFP
                     FLYPGKNEIKWTNNIHSFEIIPRWWRKV"
     gene            65437..68148
                     /locus_tag="IW797_000153"
     CDS             65437..68148
                     /locus_tag="IW797_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1222959.1"
                     /translation="MIPRIYSPTETDFSTNGLGILKDTTKCEIYEVANGKYELELEYP
                     LGTRFDEYFENDYQIKAKSNDQEEYHIFFIDDKDIDTFLDTVTIYAQSRTNRLGRRAV
                     TFAGVDSKTGREAMAIIENNMDKKSDIRLYSDITTVSSTTFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRDNIGTIRYGKDLSGLKVKLDWTGVKTRIIPYADPQS
                     DAGTTSRIYGSPVDSEYINNYPDVYTEHVQFTEEQGVKDVNSLNKIAKNYFKTINPGC
                     DKPKISITVEFDKLTDTEEGKEFAKIRNYGLFDTFKIYHRKYKLYFESKVSGVQYDSL
                     SEKVLKLEAGDAQVAFYQQQAVTIQDKLKDYATNNYMSSFNDYVSSMIAGQGNAGGYV
                     VLWPKEKPSNIFIMDSPDLNKAKEVLRMNKNGIAFSKNGWNGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSNSSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVNGKEITGNNSGGSDPGTIPPQLTTEAEKRAWKIWTMLKARGYSEYAAAG
                     ILGNIQGEVGASMNPDTEQLGGPAYGIVQWDGSAYPLVGSPTWNGREYVQRLMNTAGI
                     QEDYRSIEAQVKLLDWCMFNGQWLGKVNPTTVSGFKSINDAKSAAYAFEMNFERPASA
                     HPERQNYAQSWYNKLHGLTSPEPGGNFICPIQKPVTVTSECGWRTSPINGGQEFHNGI
                     DLVNGNPNTPVFAALDGEVVQAGANYYDWYGNYVVIKHNNGKWTGYAHLSRIDVSVGQ
                     KVQKGAQIGLMGTTGPSTGEHLHFQIMKNYWPQPVVDFENPRNYIQF"
     gene            68161..69069
                     /locus_tag="IW797_000154"
     CDS             68161..69069
                     /locus_tag="IW797_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAQ1222960.1"
                     /translation="MSKWNVVLSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            69069..69686
                     /locus_tag="IW797_000155"
     CDS             69069..69686
                     /locus_tag="IW797_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222961.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            69690..70139
                     /locus_tag="IW797_000156"
     CDS             69690..70139
                     /locus_tag="IW797_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222962.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            70139..70633
                     /locus_tag="IW797_000157"
     CDS             70139..70633
                     /locus_tag="IW797_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222963.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            70647..71078
                     /locus_tag="IW797_000158"
     CDS             70647..71078
                     /locus_tag="IW797_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222964.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            71080..71217
                     /locus_tag="IW797_000159"
     CDS             71080..71217
                     /locus_tag="IW797_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1222965.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            71254..>71525
                     /locus_tag="IW797_000160"
     CDS             71254..>71525
                     /locus_tag="IW797_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222966.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
BASE COUNT        24066 a        11704 c        15117 g        20638 t
ORIGIN      
        1 agaagtgata aaaaaacacc aaaccgtaaa aatggacatc ggcctttctg ataacagtta
       61 cttcatcaat atcgctgcag gtggctattt gacagaattg acttatgaag ttccatcaga
      121 actaaagagt atttttggtt atcttgctta tcttgcgaaa ggagcagaga tgctccctcg
      181 agtaaagcca atcaaaatgc gcttgaagta tgatgaaggg gaatatatcg gaaatgcttc
      241 gatgtttttc ctagggctga cgaactctgt tggaggattc gaacagatcg cgccagatgc
      301 caaattggat gatgggaagt tttctttaat cattgtgaag acagcaaata tctttgagat
      361 cttgcatatt gcagcactca tgctgaatgg cggtcgacat gtcgatgatc caagaataat
      421 ctataccaaa acaagcagtt tgttcgttga aacagaagat gatatcaatc gaccagtaat
      481 gatcaactta gatggtgaat acggcgggga agcaccgatg catttccaaa atcttcatca
      541 acacattgag ttctttgcca ataccgatca gattcctgat gaagcaatca caggtacaga
      601 tgaagaagaa ttagaggttg tcagcaaaga attcgtaaaa gaagtcgaac gtttaacgga
      661 tgaagatatc gacggtgacg gaaaaatagc agacgaaaca cctaatcaaa agaattaatc
      721 aaaagccggt gctgaacgta gtaaggtcag tgccggtttt agttgtgtaa ttagctgatt
      781 tatagtagag ttcagtagga ttgaaaaata ggcttaaagg agcaaaatat gacagaacag
      841 caaatagtaa agaaaaacga acgatataca gtcgaaatcg tcgacttgag ttatgaagga
      901 atgggggtag caaaaatcga tggttatcca ttatttatcg aaaatgcact ccctggagaa
      961 gaagtagaaa tactagtagt aaaagcaggc aataagtttg gatatggaaa agtcgaaaca
     1021 tttattacac aatcacctga tcgtcagcct gtagaaaaca atgcattact tcgcataggt
     1081 atcgcacctt tagcccattt gaagtatgaa caacagcttg tcttcaaaca aaaacaagta
     1141 gagcatgtaa tggcgaagat tgctaagatg ccagaagttc ctgtacttcc aacggtcgga
     1201 atggcagaac cattcggcta ccgtaataaa gcacagatcc ctgttcgccg tatcgatgga
     1261 cagttagcca ccggttttta caaaaagaat agccatgatc tagtccctat cgaagatttt
     1321 tatatccaag atcctgtcat cgatcaagca atcaaagtag tcagagatat cttgcaacgg
     1381 ttccaagtac gaggatataa tgaagaaaaa aatgaaggac agcttcgtca catcattatc
     1441 cgtcgtggtc attattccca tgaaatgatg gtcgttttag ttactcgtaa agaaaaattc
     1501 tttaaatcca aggaaattgc tgaagctatt catgaagaat tgccagaagt ggtttcggtc
     1561 atccaaaata tcaatgaaga aaaaacaaat gtgattttag gagacaaaga aaaagtttta
     1621 tatggcagag actatatcga agatcagcta ttaggaaaaa cctatcgaat ctcagcaaag
     1681 tcattttatc aagtaaatac tcaacaagca gagtatctgt accaaacagc catcgagttt
     1741 gccgagttag ataaaaaaga cattgtgatc gacgcttatt caggaatagg tacgattggc
     1801 ttgtctttag cagacagagt ggataaggtg tatgggatgg aagttattcc agaagcaatt
     1861 gaagatgcac agttcaatgc tttaaataat aaaatcgaaa atgttcatta tgaagtcgga
     1921 aaagcagaaa aagttatgaa aaaatggcaa gaaaaaggga tcaaaccatc tgtgatcgta
     1981 gttgaccctc cacgaaaagg attagatcat cattttattg aatcagcaat cgaaatgtca
     2041 ccagaacgaa tcgtctatat ctcatgtaat ccggcaactt ttgcaagaga tgctaagtta
     2101 tttgcagaag caggttatgc cgtgaagaaa gttcagcctg tagatctttt cccgcaaacc
     2161 catcatatcg aattagtcgc ttcattcgtt aaaggagaat aaaaatggac caaaaagaaa
     2221 aaatgaaaga attgatcaaa aagaaacaag gtggaggccg atccaaacaa atggaaacac
     2281 ctaagaatga tcgtaaaaac atgagaaaag gccctaaaat ctttaataaa tagacgtgct
     2341 aaagaaagac taagaagtgt taacttttgt tattgtttct tagtcttttt agttagtcta
     2401 cattattttg gcgatttgcc aataaggact gttgagttgt gcaaaatcaa atctgggtca
     2461 gaatgaacgt cattctgtct cagatttttc gttatttcaa tttgattaac tgattgatca
     2521 aaaagatttt tattttcata ttctcaaatg attcaacact aaatatacat gtttgacgcg
     2581 gaaattatta ttcgattttg gcggcatcag gggggatgac gacgtagcag acccattatt
     2641 ttactcaaga aaatgtccgt gcctattatt tagaatacga tagtgaacgt gctgggagtt
     2701 ttgttctttt agagaaagtc tctgaagata aattagttgt cttagatttt gactgtaacg
     2761 aaaaatggcg aaatggaaga gcaccagcaa gtgattgacc ggatctacga agcgacacga
     2821 tatctgcctt tggatcgttt gtgcttaagt cctcaatgtg gatttgcttc aacagaagaa
     2881 gaaaatgttt tgtttgaaga acaatggaaa aaatttcttt tgtcaaaagc attgcagaag
     2941 aagtttgggg aaaatcatta aatcatctag gagagcttga taggaaataa cactatggta
     3001 taatcagaat gcttaatact aactactgag ggagccttat gaaaaagaaa attcttaaat
     3061 ttatcggatg gttcttcgga atcattcttg ggatcgcgtt tgcaatcttt cttgccttcc
     3121 agctttcccc cagacctgga gcattcttga tcaaccatat gttttcaaat actgttcaaa
     3181 tcactgataa aaaaacgttc gatcaagcag aaaaaaacgt ggcgaaaaaa acagatttga
     3241 cgtatgagtc caagtataaa gagaatactt atgacatcta ctatccaaaa aacagcaagg
     3301 ggcctgttcc tgttctattt tgggtacatg gtggaggatt cgtcggcgga gataaatcgg
     3361 gtgtcaaaga atttgcgaca aaattagttt cagacgccaa catcgcagtg gttgcgatga
     3421 attatgaatt agcgcctgat tcagaatacc ctaatcaagt tcttcaagtg aatgaactga
     3481 tcaaagattt attagctgaa gaaaaagacg acaaattgct agacatggaa caattatttt
     3541 ttggtggaga tagtgcaggt tcacaaatcg cattgcaata tgcagctata caaacgaatg
     3601 atgcgtatgc taaacaaatg aaaatcaaac atctacttcc aaaagatagt ctgaaaggaa
     3661 ccatttctta ttgcggacct gttgatttga agctaacagc acagcagcat tcggatgatc
     3721 gatttatgaa gtttttcgtc aaaaccgttg cgtggtcgtt gattggaaca aaagattgga
     3781 aaacgagtga gcaattgaaa gaagctagtg tggctgacca tgtttctaag gaatttccgc
     3841 caacgtatat aactgacgga aatgcatact cattccagga gcaaggtcaa gcattagaaa
     3901 atcggttgac agaattaaag gtacccgtgc aatccttgtt ttatacagat acaaaaaaag
     3961 agattaccca tgaataccaa tttgattaca cgctaaaaga atcgcaaaat tgttaccagc
     4021 agacattgga ttttgtgaat aagtataagt aagaggttgt gaaaaagccg tttcgttttg
     4081 agtatgaaga aacgaagcgg cttttcacaa cctctctttc aatacctttt tgttcttggg
     4141 ataaaacttt ccctaacaat caatcgtgca ggataagtca aaatgattgg ttcgtcccgt
     4201 ataccgtcaa ttcgctccaa tgccattcga acagctgctt gcccaattga tttcgcattg
     4261 atttgaacac tggtcaagcc aggggagagg tttgcgattg tctcgatatt atcaaagctt
     4321 gctacaacaa tctcttctcc aatttggata tttttttgtt tcaatgcccg atagacacca
     4381 attgccattg gatcgctccc gacaagaatg gcagtaggaa gaggggattc attttctaat
     4441 agattagtcg caagtacctc accttgtttt tctgtccagc cacctagatg ataagacata
     4501 tatttttcta agtgatgatc gatcataaaa tgctgatagg cacgcatcct cttttcattt
     4561 atagaaatct gcttcccacc gaattcatcc atatcgactt tatatccccc gatataggct
     4621 attcttttat gaccattttc taagaacaat tccagtacgg ttttagtcat ccgttcaaga
     4681 tctgcataca ccatatcgac ctcttgctgg atgtcagggt tatccacgac gactatttgc
     4741 atgttttgtt ttaacaaggc ggtaatcgat tctttcgtaa cagtcccaac aacgatcacc
     4801 gcacctaact gatctagatc ttcccattgt ttaggattat cagatagatt atagattcga
     4861 ttcatcccta aatgcagacg tcgagcttct tcttccatcc ctactcgcaa atgtttgtaa
     4921 tagatatcct gttcttcgct cgctttgccg atcgtagaga tcacaccaat cgaaggcatt
     4981 ctctttttga tatatttacg agtactgatg tcataatcca acgcagcagc tgcctctact
     5041 atccgttgcc gagtagtctg agccacatgg atcgtctgat cgttattcaa cacacgggaa
     5101 actgtagcag gagagacatt tgcctttcgt gcgacatctc taattccaac catttttaca
     5161 cactcctcaa aaaaataaaa aatttttatt gacaaaattg tttagtaaac agtatacagg
     5221 aattataaaa aaaagtaaac ggtttccgtt ttgttgggag tgaaatgatg gatattacgc
     5281 atagaaaaac agataaaaat ttcatcttat ttgatgaaaa gagccgagtc ttccatttac
     5341 gaaatacatt tctttcttat ctgataaaga tagaagaaag caatgtattg gcgcatattt
     5401 attttggcaa accagtcaag cagtacaaag acaataaaaa ttatccccga agagatcgtg
     5461 gattttcagg aaatgttccc ttaaatcctg accggtctct gtcgaaagat acactaccgc
     5521 aagaatactc ttctcatggc agcatggact ttcgaacgcc cgcatcgatc attcagcgca
     5581 agaatggatc agacttactg gatctgagat atgacagcca ttatattaca gatgggaagc
     5641 cagagatcga aggcttgcct caaacttatg tgatggacaa gtcagaggca caaacgttgg
     5701 tgatttcact taaggataga gaaacagcga tttatttcga tttgttctat accatattta
     5761 ctgatagagc agtgatcaca cgatctgtaa agataagaaa tgaaaccgga gaaacaatca
     5821 aactagaaaa agcggctagt ttccagctag actttgccca tactaggcga tttgatgaag
     5881 tcattgcttt gccaggagca catgtcaatg aaagacagat ttcacggcaa tctgttttat
     5941 ctggaacaaa agtatttgaa agccgtcgag gaacgagcag tcaccatatg aataacttta
     6001 ttgctttagt tcatcatcat acgacagaaa atactggaga agcgatcgga cttcaatttg
     6061 tttattccgg caaccattct ttcgaacttg aaaaagatca gatcaatcaa ttgagagtcg
     6121 tcggtgggat caacagccat cgtttttcgt gggaattgaa tgcaggccag tcttttcaaa
     6181 cacctgaaat gatcctttct tactcaagtc agggattgaa caaaatgagt cagatccacc
     6241 atgagttgct acgtgaaaga atcgcaagag gtcgtcatca atttgctgaa cggccgattc
     6301 tggtcaataa ctgggaagcc acttactttg acttcaatag tgaaaaaatc aaggcgatca
     6361 ttgatgaagc aaaggagcta ggcatcgaaa tgttcgtatt agatgatggc tggtttggaa
     6421 aaagagatgc ggacaattct tcattaggag actggttcga gtatgaaggc aagttgacaa
     6481 atggattacg tgaaatagca gattatgctc atagcaaagg attgaaattt ggtttatggt
     6541 ttgaacctga aatgatctcg gttgattctg aactgtatcg aacacatcct gattttctta
     6601 tgcaggagcc aggtagaatg ccttctgctt ccagatcaca acatgtactg gattttacaa
     6661 ggttagatgt tcggcagacg attgagaaac aaatgagaaa gattttagat acgattcctc
     6721 ttgactacat caaatgggat atgaaccgtt cgctttcaga tgtctattcg attacccttg
     6781 acccacagag acaaggagaa gtagcgcatc gttatatgct cgggctttat gaactacttg
     6841 aacatttgac aactgattat ccggaaatct tatgggaagg ctgttctggt ggaggtggac
     6901 ggtttgatgc cgggtttatt tattatatgc cgcagtcatg gacaagtgat aacacggatg
     6961 cagtggagcg tatgaaaatc caatatggaa caagccttgc ttatccgatt tcgtccatca
     7021 cagctcatgt atcagctgta ccaaatcatc agaccgacag atctacttcg ttgaaaacaa
     7081 gaggagaaac agctatgagc gctgtttttg gttatgaact ggatttgaca aagctgtctc
     7141 ctgaagaaga aaagcaagta aaagaccaaa ttatatccta tcaaacgatt cgtccggtga
     7201 tccagtatgg ccattattat cgtttggcga gtccttttga agaaaatatt gccgcatgga
     7261 tgtttgtgtc tcctaaacaa gatgaagcta ttgtgttttt agggaggata ctagcttcag
     7321 ctcaaccagc gttccatgaa gtatatctga tggggttaga tgatgaggca ctttatcagg
     7381 aacagacctc gaagcggata ttttcggggg ccgaattgat gacagttgga ctttacttcc
     7441 ccgattttca aggtgatttc caaacagaac tgcttcattt caaaaagtta tgagagagaa
     7501 ggaaaaaagt atgaaagcaa atataaaatg gttagatgat ccacaagttt ttagagtgaa
     7561 ccaatcaaat gcacatagcg atcatgtttg ttatgcaaca agggaagaag tagaaaccac
     7621 atcctccctt aaacagtctt taaatggatc atggcgtttt tgttattcgc caaatccttc
     7681 ggtacgacca aaaacatttt ataaaaaaga ttttgatgcc tgttcttttg atgaaatcac
     7741 tgttcctagt catattgaat tagcaaatta tgacaagatc cattacatca atacgatgta
     7801 tccgtgggag ggacatgtat ttcgccgtcc ccctttcacg acaagccctg agaaagaggc
     7861 ggaagggatc ttttcagctg ctaaagacaa tccggttggt tcgtatttga cgacttttcg
     7921 attagaagaa ggtctaaagg gaaaacaaat ctcgattcat tttgctggtg tagaacaagc
     7981 aatgtttatt tggttgaatg gtcattttat cgggtatgcc gaagattcgt tcactccgtc
     8041 tgaatttgat ttaacacctt atttggaaga agaaaatatt ctagctgtgg aggtttacaa
     8101 aagaagtaca gctgcattct tggaagatca ggactttttc cgattttttg ggatcttcag
     8161 agatgtcttc ttagttgctc gaggacaaaa ccatttagaa gatctttgga tcaaacctcg
     8221 cttagatgac aaatgtcaaa atggcaaact tgaagtagat ttgtcttttg agcaagtagc
     8281 caaaaacagt cagctgaatg tagcgatact agatcctgat gggaaggaaa tctatcaaca
     8341 gcaatatccg ttaaaagagc aaatgacgat tcgcatcgat ggatttgaaa atgtggcact
     8401 gtgggggcat tttcagccca atttatataa gcttgaattg gagacgatag acgaagaaaa
     8461 aagagtaacg gagtatattg actattcctt cggttttcgt aaaatcgaaa tcaaagataa
     8521 aatcatctac ttcaatggaa aacggatgat tatcaacggg gtaaatcgac atgagtggca
     8581 tccgaaaaaa ggacgcgcca tcactataga agatatggaa aaagatttgg ctatcataaa
     8641 aaatgcaaag atcaacggcg tacggacatc tcattacccc aaccagatca cttggtatta
     8701 tttatgcgac caagcaggaa tccatgtgat ggcggaaacg aacctcgaat cccatggctc
     8761 atggcaaaaa atgggcgcat ttgagccttc ttacaacgta ccaggtgcag tacctcagtg
     8821 gcaggcggct gttcttgatc gagcaaaaac gaatttcgaa acattaaaaa accatccaag
     8881 tattcttttt tggtctcttg gaaatgaatc ttatgcggaa gaaaacattc gacaaatgaa
     8941 cgattacttc aaaaaagtag accccgatcg acttgtccat tatgaaggga attttccaaa
     9001 tccagcattc gaagattcga tttcagatgt aaaaagctgc atgtatgcgc caccagaaga
     9061 gatcattgac taccttgaaa ataaagcgga aaaaccgttc atcttatgtg aatatatgca
     9121 tgacatgggg aattcgttag gtggaatgga ttcttacatg acgttgcttg atcgttatga
     9181 aatgtaccaa ggaggctata tatgggattt taaagatcag gcgatccaag tgactgatcc
     9241 aatcacaggc agagaagtga tgcgctatgg cggtgacttt gacgataaac caagtgacta
     9301 tgaattctca gggaatggga tcgtttttgc agatggacaa gaaaaacccg ccatgcagga
     9361 ggtaagatat tattatgaaa aatacagtaa ataaaagtca tatggatacg gaaaaagttg
     9421 caatcgtctt cggcgactgt acattaggtg tcaaatcggg gaatacgcat tatatttttt
     9481 cttatacaag aggcggactg gaatcgctca ataaaaacgg aaaagaatgg ctataccggg
     9541 aaacacttcc agttttttgg cgagcaacga ccgataatga cagaggtagc ggatttggat
     9601 ttaaatccgg tatgtggtta ggagccggat tgtatccaaa agtcaaagaa atagaagtac
     9661 tggtggacaa taaaaagatc gatttgccga ttgctccagt aaataatcaa tattcaagta
     9721 atgaatacgc tgaaacggtt gaaatcattt ttacactgga aatcaacaca gtaccaaaaa
     9781 cccaagtgac aatttcctac caaatcgctg gagagggtaa aatgcttgtc aatatgcatt
     9841 attatggaca aaaaggatta ccggaattac ccgcattggg tttaaggttc attatgccaa
     9901 cgaaagcaaa attttttgag tatgaaggcc tatcagggga aacatatccg gatcggatgg
     9961 ctggtggtgt cgaaggtatt tataaagtgg caggactccc cgtttcccca taccttgttc
    10021 cccaagaatg cggcatgcat atggaaacga aatgggtaga actaacaaga aatcaaacgt
    10081 tggataatac ggataaagat caagaagatt tttcgttgcg atttgaacag caggatacgc
    10141 catttgcttt ttccgcttta ccattcaaag cagaagaact ggaacaggca acacatatag
    10201 aagaactacc gccagaaaga cgaacggttc ttacgatttt tgcaaaagta agaggagtcg
    10261 ggggtatcaa tagctgggga gcggatgttg agtcacctta ccgaatcagc agcgaagagg
    10321 accatgaaat cggctttatt attcagtagc aatctttaga ttattatttg aaaaacggag
    10381 tttacaagac aaagctgctt tattctatat gtcatgaaca agagagtaaa aaagttctca
    10441 gatacttttg ttcatgacta ttttcttcaa taaaaccaat tgttattttt ctgaaaaaca
    10501 aagttaacta tcagaaaatt aaaaaaagtt tcataaaaat gattgacagt cgattttttt
    10561 atgtctatac taggtacaat tcttacatca gtttggagcg attttttatg aaatctagaa
    10621 aacttacaaa actgaatacg tattacttta gctattttag ataggtttgt attcatgact
    10681 tcgtgggtac aaacaaacca ggtttgtatc tatgatggct aaagattttt aaacacgact
    10741 tacagccacg tagagaaatt catctatagt ggctatttta tatttttgca gcgcttttta
    10801 aggctcgttg aagatgttca ctgtagaggc aatttcctac agggacatct ttttttgttg
    10861 attatctggg tcataagtag gaaagaagga gcgaaaatga gaacaacagg acacggtcat
    10921 ttagtaggaa ttaatgtaaa accaccttga aaaagtagag aaaatcagcc aagatagata
    10981 agtaaacaaa agaaagcggg aaaaacaatg atagtaatta tgaaaaaaga agcaacggaa
    11041 gaacaaataa aaaaagtcgt cgatcatatc gaaaaagaag gattcaaagt ctcaatcgat
    11101 caaggaacag aacgagtagt gattggacta aaaggtgata cgcgaagttt acaagaagga
    11161 gcatttacac gctatgaagg agtagaaaat gctgtgcgta ttttaaacac atacaaattg
    11221 acttctcgag aattccatcc tggcaataca gtcgtggatg tagacggagt aaaaattggt
    11281 gacggcagtt tcgtgacgat ggctggtcct tgttctatcg aaggattaga ccaaatcaga
    11341 gaatgtgcgc gaatggccaa agccgggggc gcaaaaattc tgcgaggtgg tgccttcaaa
    11401 ccacgtactt ctccatatgc tttccaaggt ttagaagaag aaggattgaa atacatccgt
    11461 caagcggctg atgaatttgg aatgaaagtg attactgaag tgatggatga agggcatatc
    11521 gatatggtgg ctgagtacag cgatatccta cagattggcg cacgtaatat gcaaaatttt
    11581 aaactgctat ctgctgtcgg taaaacaggt aaacctgttg gattgaaacg tggaatttcc
    11641 ggtacgatca atgaatggct taatgctgca gaatacattg cagtcgaaga caaatctcct
    11701 gtcattttta tcgaacgtgg gattcggact tacgaaacag cgacacgtaa tacgtttgat
    11761 ttaagtgctg tcccattgat gaaaaaatta actcatttcc ctgtgattgt tgatccaagt
    11821 catggtacag gaatttggga acttgtacca ccaatggctc gtgcgggtgt ggcaagcgga
    11881 gcagatggca tgatcgtaga gatccatcct gatcctgcaa atgcttggtc agacggtccc
    11941 caatcgttga atgaaaagac ttactcacgc atgatgaaag aagttgcgat catgaaagaa
    12001 gcaatggaaa agattcagac actcgattaa ttccagcttt ttaaaagaaa gaagtgaaac
    12061 gaatgcttac tgtacatgtt gataaccaaa aatacgatat tcatattgaa aaaggcgcac
    12121 tagaacaggc tggatcatgg cttcaaacga tttggagacc tcaaaaagta gctgtgatca
    12181 ctgatacaaa cgttgctcca ctatatgcag aaaaaatcat gttccaattg agacaagcta
    12241 attttgaacc agtcctttca atcgtaccgg cgggtgagac cagtaaatct ttagaacaag
    12301 cagctgagtt atacgatttt ttagcagata atgattttac ccgcagcgat gggatacttg
    12361 cacttggtgg aggaatggtg ggtgatctag cagcattcgt ggcctcgacg ttcatgcgtg
    12421 ggatccattt cgttcaaatt ccgactacgc tacttgcaca agtagattct agtattggcg
    12481 ggaagacagc agtcaataca aacaaagcga aaaaccttgt cggaacattt agccagccag
    12541 ctggtgtatt gattgatcct gaggttctaa aaactttgcc ggtacgtcgt ttacgagaag
    12601 gaatcgctga aatcatcaaa tcagcagcta tagcagatgc aacgctttgg aaacagctga
    12661 acgagctgaa agacgaaaaa gatcttctgg aacatgctgt tccaatcatt actgctacgt
    12721 tggaaatcaa acgaaaagta gtggaagaag atgtctttga ccaaggaaat cgacttattt
    12781 taaattttgg tcatacaatt ggccatgcaa ttgaaaatac agcaggatat ggggtagtca
    12841 gtcatggaga aggagtagct attggaatgg tagccatcac ttctcatgca gaaaaaatcg
    12901 gcttgtctcc aactggatcg acagctgctt tagaaaggat acttcaaaaa ttccatctgc
    12961 cgattacgta cgatctaagt catccagatg aattattcca cgcgataacc catgataaga
    13021 aagcaagagg aagccaattg aatatcatct tgctagaaaa gattgggcaa gcaaaaatcg
    13081 ttcctgttcc cacagagaca ttcaaagatt acttaggagg actgtaagat gcgttatatg
    13141 actgcgggag aatcccatgg accagaatta acagccatta ttgaaggact accagcagga
    13201 ttacctctgt cagaagaaga aatcaatcac gaactcgctc gcagacaagc tggttacggc
    13261 agaggaggaa ggatgaagat cgaaaaagat cgtgttcgga tcacgtctgg catccgtcac
    13321 ggcaaaacaa ttggttcacc tgtcacttta gtggtagaaa ataaagattg gaaaaattgg
    13381 acaactgtca tgtctgtagc agaagtgcct gaaaaagacc aatcactgcg gcgagttgca
    13441 cgtccgcgtc caggacacgc ggatttggta ggcggtatga aataccatca tcgagatctt
    13501 cggaatgtgc ttgaacggtc ctctgcaaga gagacgacca tgcgtgtagc aatcggcgca
    13561 attgccaaga aaattctgga agaacttgac atcatcgtag caggacacgt gatttgtctt
    13621 ggtggaatcc aagcagaaat acctggaaat atctcgattc aagaaatcaa agaaatttct
    13681 gaagcgtcag aagtacgagt tgttgatcca gcagttgagc aaagaataaa agatttgatc
    13741 gatcagacaa agaaaaatgg agatacgatc ggaggaattg tagaagtgct cgttggaggc
    13801 gttccagctg gattaggaag ctacgtacaa tgggacacga agctagatgc caaaatcgca
    13861 caagctgtgg ttagtatcaa tgcctttaaa ggcgtagaat ttggggtcgg attcacttct
    13921 ggttcattac caggctcaga agtgatggat gagatcctat gggaagaaga aagaggctat
    13981 ttccgacgta ccaataatct aggcggcttc gaaggcggaa tgaccaatgg cgaaccactt
    14041 gtcattcgcg gggtcatgaa acccattcct acattgtaca agccacttca aagtgttgat
    14101 atcgatacaa aagaacctta caaagcaagt gtagaacgtt cggatagtac agcagttcct
    14161 gcagctagcg tcgttgcgga aaatgttgta gccactgtgg tagcgacaga gctattgtca
    14221 aaattcccaa gtgattcgct tgttgagttg aaagctgcag tgaaggagta ccgtgactac
    14281 ttgaaacatt actaaaaagg gggcaaataa gatggcacaa catgttttta tcgtggggct
    14341 tggactgatt ggtgcttcta tggcactttg tattcgtcag accaatccag aactgatagt
    14401 agttggttgg gatagtcagt caaaaacccg tgagcaagca gtagaacagc agatcgttga
    14461 ttacgttgca agtgattttg aaacaggtgc agaacaagca gacgttatct tattagccgt
    14521 tcccgtccgt acaaccttag cttatcttga tgtccttgaa agaatcaact tgtctgatca
    14581 cgtcatcatt acggatgtca gcagtacgaa gcagcaagtc gtgacatccg ctgagcagaa
    14641 aaatcttcgg tttgtcggtg gacatccaat ggctggttct cataaatcag gtatacaggc
    14701 agcagatgct aaactatttg aaaatgccta ttacatcttt acacctttgg aaaagacgaa
    14761 aaaagatgtt gaaaaactgc aagaactatt ttgcggaacc aaagcaaaat atgtaactct
    14821 tacagcaaga gaacatgacc gtataacagg aatgctaagt cattttccac atattctggc
    14881 agctggctta gtcaatcagg cagaacaatt caatcaagag tatccgcgag ccaaacaact
    14941 ggcagcagga ggctttcgag atatcactag gatcgcttcc tcagatcctg tcatgtggac
    15001 agatattttg ttgagcaata agcagatatt attggaacga ttggcagatt ggcaacaaga
    15061 aatgacacaa atagcagaat ggatcatgac tgagaaccaa tcggaaatct tttctttttt
    15121 caatcgggca aaagaaagtc gagaccagtt gcctgtccat aagcaaggcg cgattcctgc
    15181 cttttttgat ttatttatcg atgtgccaga tgaacctggc gttattgcag aagtaactgg
    15241 actgatcgga aaagcgggag tttctttgat taatttgaag atccaagaga ccagggaaga
    15301 tattttagga atactgcaaa tcagttttaa aaaccaacag gatttgttac aagcaaaaag
    15361 aatcattatg tctaatacca gttatcattg ctggatcaaa taggaggctg gataaatgca
    15421 attattacaa caaatacatg gattaagagg gactgttagg ataccagcag ataaatcaat
    15481 ttcccatcgc agcatcatgt ttggagcaat tgctgaagga acgacgacta tacaaaattt
    15541 tttgcgcgca gaagattgtc tgagtacttt acatgccttc caacaattag gtgtcgagat
    15601 cgaagaagag gaagaggtga tcaagattca tggtcgcggt agccactcat ttgtccaacc
    15661 aaccgaatca attgacatgg gaaactccgg tacgaccata cgtttattga tgggtatttt
    15721 ggctggacag ccttttacga ctactctgtt cggggatgct tcgttgtcta aacgtccaat
    15781 ggggcgagtg atggaacctt tacgcgagat gggcgctgac ttgcaaggag aagaaaatga
    15841 ccagtatcta ccaatcactg tgacaggaac ccgctcttta tcacctatcc gatacaatat
    15901 gcctgtagcc agtgcgcaag tcaaatctgc tttgctgttt gcggcattac aagcagaagg
    15961 cacatccgta atcgttgaaa aagaacgttc ccgtaaccat acggaagaaa tgattcgtca
    16021 atttggcgga aggatcacag tggaagataa aacgatcata gtgaccggac cgcaaaaatt
    16081 aactggtcaa cagataactg ttccaggtga tatttcatca gctgctttct ttctagcagc
    16141 aggacttctt gttccggaaa gccaactgtt gttaaaaaat gtcggggtca atccaacaag
    16201 aactggtatc ttagatgtgt tggaggagat gggcgcagcg attacccaga cgaatcacaa
    16261 tgaacagaac caatcggctg atttaagtgt caagacgagt catttgaaga aagcaacgat
    16321 tggtggagaa atcattccgc gtctgattga tgaattgccc attctagctt tagtggctac
    16381 tcaagcagaa ggaatcacga tcatcaaaga cgcagaagaa ttaaaagtaa aagaaaccaa
    16441 cagaattgat gcggtggcag aagaattaca gaaaatggga gcaaagataa aagcgacagc
    16501 agacggtctg atcatccatg ggccgacacc tctgcatggc gcaaaagtgt ccagcagagg
    16561 cgatcatcga atcggtatga tgctgcaagt agctgctttg atagctgatg gaccttgcga
    16621 actagaagga gctgaagctg tttcaatctc ttatccggca ttttttgacg atttagcaga
    16681 acttgtgtca ggaggagaag ctcatggcta gcattttgct gattggtttc atgggcgctg
    16741 gaaaaacaac aatcggtaaa gggttagctc aatgtttaca aaaagagtat gtagatttag
    16801 atacaaagat tgaggaacat atccaattgt cgattgccga atattttcac tattacggtg
    16861 aaaaaagctt tcgaaaagtg gagtcagata ttttaagaaa gctgtcaaat gaagacaaaa
    16921 ttatcgcgac tggtggaggg attgtccaaa gtgcagaaaa tcggcgtttt ttaaaaacac
    16981 agccgattgt tctttactta gaggcagaag cggactgtct agttgatcgt atccaacaag
    17041 atgaaacgtc tattcgacct ttagctcttg gaaaaacaag agaagaaata aaagcattat
    17101 tggcacaacg actttcttgg tatgaagaaa gtgcgacaca tcgaatcaac acgaccaatc
    17161 aaacaccaga aaagatcatc gataccatta tagaaaggat caaaagcata tgaacgtcag
    17221 ctatctaggg ccagaaagtt cattcactta tcaggcggct tgtcagctat ttcctacaga
    17281 acaattgact gcgtatgctt ccatccctgt ttgtttgcgc gctttgtttc gcaaacaagt
    17341 cgatctggct gtcgttcctg tagagaattc attggaagga gctgttcatc acacaatcga
    17401 tcttctttcc aaacatccag aagtcgaagt taaaagtgaa atcgttcttc ctatcaagca
    17461 gcagctttta ggcaatccag caacaaagat tacaaaaatc ctttcacatc cacaagcact
    17521 agcacaatca caacagtttt tagaaacgca ttatcctaat gttcctttag tagcaacaga
    17581 atcaacaact gctgcagcaa tgtatgtagc agaacatccg aaagaagacg cagcagcgat
    17641 tgcttcatta gaaacagcac agcatgttgg attggagatc ttagctgaga atatccaaga
    17701 taatgagttg aaccagacac gtttttggat cgtcggtgac cgaaagatga ccagccagca
    17761 gtcagctcca gtaaaaatga gtgtcatctt gacactacct gctaatcggc cagggatgct
    17821 tcataaaatg ctggcagctt ttggctggcg ggaaatcaac ttaagtaaga ttgaatcccg
    17881 accattgaaa actagcttgg gagaatattt tttcgtgatc gatttgttgt tggaccgtcc
    17941 aatgacatta gtagagaatg cgctccaaga aatcaagatg ctcggaggag aaagtcaaat
    18001 attgggctgt tatccagtgc tgacagtaga agagacgcgt agatgaaagt caagaatact
    18061 gattgattta tttaacttaa aataaaaaaa tcagcgcttg gctattcttt atttttgata
    18121 ttctgattga tttttaggaa gaagcggtag acaaatcgtt acagtcgttc ctgtcccaat
    18181 cactgattgg atcgttaagc catatgcatc gccatacatt aggcgtatcc gctcatctac
    18241 atggctgatg ccgattcctg aaaaatgatc atttttttgt ttggtatttt cagtatcgaa
    18301 gccatcgccg ttatcaatca cttcgaataa aagaagctga ttttttgttt tgccgtacac
    18361 cgtgatgaat ccttgcgttt tattgtgaaa agcatgaaga taggcgtttt cgataattgg
    18421 ttgaaggatc atattcggca tagataatga ctgcagttca tcaggaatca ttattttagc
    18481 cgagattctt tcgccatatc gcatactcaa aagatcgatg taactttgta cgcttttgat
    18541 ttcttccttg actggaatca cttcttcttt tttcccaatc gtacttctta gtaaagtaat
    18601 gaaactgtcg attccttgaa ctgcttcatc tgtttttttc tgccaaatca aaaatttcaa
    18661 gctagtcagc gtattataaa taaaatgggg ctgtatctgt gcttgcatag tttggatttc
    18721 aagcgaacgt ttcttctttt cgtcttggat cgtctgttca taatgatgct gagcattact
    18781 tagcaattga ttgatcgtat tccctagcaa ttggacttca tggcttcctt gtacaggaac
    18841 agtagctttt aagccatctt cctctagccg ttttagcgaa tcaatcaagc ggtagatcga
    18901 ttttgtgtgt cgatccaaat acataaatac caatgagcca gcaaaagcaa gtgtcacgat
    18961 attatacaaa ataatcggaa aaatcacgcc catttggtta gataaagaat gaagatcgac
    19021 ttgactgagt aatgtacaat cctggcggta cagaggaaga cgggtcaact gctgtccatt
    19081 tgaaatcgtt gtttcaccta ctgttagttt tttgaaaatc attgatcggc tcccaattgt
    19141 attttcttca tttgatgcga tgatcgtctg ttcttcgttc atgaaggaga tcttcgatat
    19201 ctcaggattt actacagatt gaaaaagatt cgctagttgc ttggatgaga tcgataaaac
    19261 agcatatccg taaacgtgat tcaatgtatt tttcagtttt ttgacaaaga agacgctggg
    19321 tgctttctta tcgcgaagag tcaatccgtg gtgtgcaaaa agcatttgtg tatcagcagc
    19381 acgttcattc actctctgag caatcgcaga ttgaaaaaaa gaatcggcag atacggcagt
    19441 gagcatgtcg tttccgacaa aagttcttcc attttctcca aagatcaata catcgtaatc
    19501 gatcattgga tggatttttg aaagagagtc attcattttt tggattgttc ttagttgctg
    19561 aagctgttcc atatgttggg ctggatcatt ggaaaaatag cttttgacag ctggattaga
    19621 atcaaaagcg gctaatgtat tctcgattgt atcattcaaa ctttcaaatt cagattgaat
    19681 cttttttagt accacatcat tcgattgatt gaacagctgg atataggcat cccttgatcg
    19741 atgataaata aacaaagaaa tgacgatggc ttgcagaaaa agaccgatga acaaaatgaa
    19801 cagaagtttg gcaaagagtt catgctcttt gatttttttt ctcattttac aaaagttcct
    19861 ttacggtatt gagaaggtgt cattcctgtc tcttctttaa aaactctgga gaaataaccc
    19921 agttcggtat agccgacttt ctcacagatt tccgtcaaag taagccgtgt ttccgtcaaa
    19981 aagagcttgg ctttactgat acggactttt tttagatatt ttgaaaagtt gataccaaat
    20041 ttttcagtaa agatggctga gagatagcta tatgaaaaat gatatttttc ggataaaagt
    20101 tgtaaattga tttctttctc ataattttcc tggacgaact cgtagatcaa agtcaagata
    20161 tcgaaattgt ctttttttgc atttgcagaa aagtgggtga gtttctcaag tgtttctttg
    20221 acaagaatag aaaaatcttc gaaatactca gcatcggcta ataaagaagg aagggcgatc
    20281 ttgagcaaag aaagttcttc attcccaaca tgctcgtcag ccaaagcgtt gagtaatgca
    20341 tagaaacgat tgattgcctc atgtttcaaa taagctggat caataggctg ttccaatatc
    20401 gtcgtgaaga aattctcaag ttctttgaca gctaaagcgt agtctttatg aacgattttt
    20461 gttaaataac tacttgcttg ctgctcgtgg ttggttaata acggaagaag ttcatgctga
    20521 taagcaaggt tctttccctt atgatagaaa cgttgtcctt tggtgcaatt tctgatctct
    20581 tggaatattt cattgatatc ttggattgca aaagaatcag aaacaacaaa aaaatcacct
    20641 ggaaaaactt cttgacgaaa cttttgaaca aacttcctaa gtgtatcttt ggaatcagta
    20701 ttgattacat agccggattc ttccgaaaag gtagaaacag gataaatagt aaacgaatcg
    20761 tcgctatcca tcaatgaatg ggtgatcgac tgtttttgtt tagagccttg ttttgaactg
    20821 aatggccgtt tttcggtata taacagaaaa acaaggtgtg ttcaaaaaga agagggtcaa
    20881 gcaatggacg agtttgtcca gcaatctgct gtgaaagatt ctcgcagatc tgctcataac
    20941 gattggtgat tgggcttttg ttgggctttt ttttgatttt ttgtgaaacg gcagttaagg
    21001 aatttagaaa aaaatcagga ctcaatcgag gctttaaaag ataatcagcg acgccttctt
    21061 taaaagaatc tttaaccata tcaaattcat catagctaga gaggataagg atctgtgttt
    21121 caggttgttg ctgttttaat agccgcgata attcgattcc atttaattca ggcatcatta
    21181 catctgtgac cacgatatcg attgcatgta ccaaagaaaa atcaagcgct ttcagcggac
    21241 tggaaaaagt ccctattaat tgaaacccgt gctcagacca attgatcaac tgtttcattc
    21301 catctaaaat cgtctgttca tcatcgataa gaattacgcg tattggcatt tttttacccg
    21361 ccttaagaat agtcttgtaa tttaaaaata cttatttcta ggagaaaatg caaggatatt
    21421 tttattttaa agaaacctca ccatttcgat ctttggtcta gatgctaaga ttattccttt
    21481 tgtcatagtt gatttgtatt tttttattat tttaggagaa atttttgaag tatctgaata
    21541 ataaagaaac cgttttcaaa ttttggtatg atgaatgtag caaacgtaaa caactaatgg
    21601 atggaaaagg agcggataaa aatgaacatc agaaagttaa ttgctggagt cgtttgttta
    21661 gcggcagcat caggtttctt aacaggatgc ggaagtgcta gtagtacagc agacaaagaa
    21721 gaaaaagtca ctgtctgggc gtgggatgag acattcaata tcaaagcagt gaatgaagca
    21781 aaaaaagttt atgagaacga agaaacagag atagaagttg ttacgatgtc tcaggatgat
    21841 atcgttcaga aactgaacac cgctttagcg agcgggaata cagaaggatt gcccaatatc
    21901 gtcttgattg aagattatag gatacaaggc tatttgactt cttatccaga tgcattcgct
    21961 aatttgtctg ctattgtaaa ggaagatcat tttgcaccgt ataaatttgc ggtaaacaaa
    22021 gtaggagata aaatatacgg tgttcctttc gattcgggcg taacagcaaa cttctatcgt
    22081 acggatctga tggcagaagc aggttataca gaagaggata tggagaatct gaagtgggac
    22141 gagtatattc aagtagcgcg agatgtcaaa gaaaagacgg gaaagaaaat cgctgaagtc
    22201 aatccttctg accttggtcg agtaaggatg attatgcaac aggcaggaga atggtataca
    22261 tctgaagatg gggagacagt cacgatccaa gacaatgcat ctttaaagta tggtcttgaa
    22321 ttgtttgcca ctcttttgaa agaagatcta gtcgaacaaa cctctgactg gaatgctgga
    22381 gtaactgcga tacaatcagg agcagtagct tctagcccaa ctggcgcttg gtattcttca
    22441 acgatccaag gagcagaaaa ccaatctgga aaatggaaga ttgcaccaat acctgcactt
    22501 ccagaaaatc tgcaaaaagc gcaagcttct aaccttggcg gcgctggctg gtatgtgatc
    22561 aaaggagtag ctggagaaga ttcagcgaaa gatttcctag aagcaacgtt tgcttcgaat
    22621 gaagaactga tgggcactct tgcgaaagaa atcggactag tatctacgat gcttagcgcg
    22681 aatgaacaag aagcttatca agaagcttct gaattttaca gtggtcaaaa gatctttgac
    22741 gatttttcaa catggacatc tgaaatacca gaagtgaatt acggacatga gacctatgca
    22801 attgaagcag tcgtagctga agctcttcat cgaatcatca atggagaaga gacagacaag
    22861 gtactagcag atacacaaaa acaagtagaa gcacaattgg ctaattgatc ggtggtaaaa
    22921 aagtgaattt cttcgctttt tcatcagaca aacgaaaaga caaaaaagat cacgtctgcc
    22981 ttacagatat tgtctttctt gtcttttcat gtagataatc aatgatagaa ttggagggaa
    23041 aagaatgaag cagaaagatt tattcaaaaa cgggcatgct tttttaatct tgccagtcat
    23101 tttgatatca atcatggttt ttattccgat ggttttggct ttgatcacgt cttttcaaag
    23161 tggtccccct gtcaatatga catttaacgg gttaggtaat tatcagcgga tgttgacaga
    23221 tgcaactttt aaaaaagctt tttttaatac attcctgtat ttacttgtac aagtaccgat
    23281 catgttgttt ctagcacttt ttatttccaa ccttttaaat gataaaaagt tgaaatataa
    23341 agggttgttt cgaaccgcta tctttcttcc atgtatcact tcgatggtca gttattctct
    23401 gatcatgaaa agtctatttt ctcaatctgg ccttgtaaac aacttgttgt tgggcattgg
    23461 tatgattgac acaccgattg gctggttgac tcatccaatt tgggcgagag ttctgatcat
    23521 tctttcgatc acttggcgtt ggactggata caatatgatt tttttccttt caggcatgca
    23581 aaatattgat tcggagattt atgaagcagc atctattgac ggtgcttcta aatggcagca
    23641 gtttatcaaa gtgacgatcc caaatttaaa accaatcatt ttgtttactt ccattacttc
    23701 gacgatcggg acattgcagc tttttgatga agtacaaaat attactggcg ggggaccagc
    23761 aaatgcaacg accaccctat ctcaatacat ctataatttg agttataaat tcacgcctaa
    23821 ttttggatat gcggcagcag tttcctttgt tattgtttcc tttattgtga tattgtcgat
    23881 aatccaaacg aaagtaggag gagaaagtcg atgaaaaata tggtagaaaa agaaacaatc
    23941 gaacgcccca acgtcaaaaa acaagcatta gaacttcgtt cgacaattgg gtcagtatta
    24001 aagtatacag cattaagcat cctatcattt atttccattt tcccttttgt ctggatggtt
    24061 ttgggaatga cgaatacacc cgtggatatc acatcgggaa aaataaagat tggcagtaac
    24121 ttgatcatca attttcaaaa tttgttttcc aatgagctga attttactcg ttccctttgg
    24181 aattcagcga tgatcgcttt ggtgaccact gtgtttgcat tgctgatttc gtcaatggca
    24241 ggatatggat ttgagattta tcaaagtaaa cgaaaagacc taattttcaa tattctactg
    24301 ctatcgatga tggttccttt tgcggcattg atgatccctt tatatcgaat gttttcacaa
    24361 ttgaacggaa cggttttggg aatcaacagt ttcttggttg tcatcctgcc atctgttagc
    24421 actgcttttt tgatcttctt ctttcgacaa aatgttaaat cttttccaaa agatctagta
    24481 gaagccgctc gattagacgg tctaaaagaa ctaagtattt tttttagagt gtatatgccg
    24541 acggctaaaa atacctatgc cgcggctgca atcatcacat tcatgagtag ctggaacaat
    24601 tatttatggc ccttagtagc tttgcaatct cctgataaac ggacggttcc gcttgtttta
    24661 tctgctatgg gtgcttccta tacaccagac tatggaatga tcatggctgg attagtcatc
    24721 gctactttac caactgccat cgtatttttt gttttgcaaa agcaattcgt tcaaggaatg
    24781 atcggctctg taaaataaaa aaacacaata agcgcgttcc ttcaaagtca tttttccaag
    24841 taaaaagata cttttgggac gtgtgttatt ttatgttgag aaaaattaaa tgataacgtt
    24901 ttaagtttgt gctcgaaaac tctttcattt taaggtaaac ttaaagagta aaggaggagg
    24961 atacatgcta aaaaatttta aagaagtgat tgctgctata ttgccgatga ctgtcctgat
    25021 tgtcatttta acgtttgttt ttgcaccgtt agaaggagaa gacctcgctt catttctggt
    25081 tggtgcggca atcatgatga tcggaatgac gctctttttg tttggagcag attattctat
    25141 gatggaagtc ggagatctag tagggaaata tatgatcaaa aagaaaagtt tgaccgtttt
    25201 ggtcagtttg ggatttgcta tcgggatcgt gatcacgatt gctgaacctt ccgtgcaagt
    25261 tttgggacaa caagtatatg atatatctga cggagagatt ggcagagtgt tgctgattgg
    25321 gatcgtcagc gtcggcactg gagtatttct agcctttgcg ctgcttcggg tcgtattcaa
    25381 gctatcctat tatcaattga tggctatagg ctacataggc gttttgatcg cctcattttt
    25441 caccagttcc gaatttatgc cgattgcttt tgattcgggg ggagtgacta ctggtccagt
    25501 aactgttcca tttatcttgg cccttgccgg agggatgact agtatgatca aacaaaagaa
    25561 aaatgaaaat gacagttttg gaatggtcgg tattgcttcg ttagggccga ttcttgccgt
    25621 gatgattttg ggggtgatct tccaatgagt ttctggcagc aagtgataga tggaatcgaa
    25681 atcgttacca tggaagtcat tttggcagtg gcacctatcg taatcctatt tgttttaatg
    25741 aatatcatta gttttcgtat gcgcaaaaag cgtttctttg acattatgaa aggtttcttg
    25801 ataacgacta ttgggttgat ccttttcctg cacggagtca atatggctta tgtaccggta
    25861 ggacagcatt taggcagtgc tattgcggga ttgccttaca attatatact ggttcccctt
    25921 ggctttttaa tggggttctt agtagggttt gctgagccgg cgattcatgt aatgatcaaa
    25981 caggttgaag aattgagcga ggggagaatc cgatcgaaaa tcatgctgat ggtaatttca
    26041 attgggatcg gcgcagcagt aggattgtct atgtggcgtt tgctcgtcgg attctctctt
    26101 tattatttct tgatcccagg atacgggcta gcatttattt taggacgaaa agtggacaag
    26161 atctttttag caatggcttt tgataacggt ggagtagcaa cgggcccgat gtgttcgaca
    26221 tttattttat caatgagcgt ggcaatcgct gcacaaattg aaggaagaga tccgatcatt
    26281 gacgggtttg gtgtggttgc aatgattgca ttgacaccaa tattatccac tttagtacta
    26341 ggatatattt ataaacgaaa agatgaaaaa gacagaatcg aaaaagaaag attgcaaaaa
    26401 ttgggggaaa gcaaatgaaa gatcttgtag cattgaatct tcacatgatt gtgacgatcg
    26461 ttgacggagg aatgggcgaa gatgttatcg acttttcaaa agaagcaggt gcagcaggcg
    26521 gaacgatctt acatggaaga ggttctggtg tacatgattc agggaaattt ttaggtttgg
    26581 acatttcgcc agaaaaagat attgtgttga cattagtacc cgatagtttg acgaataaag
    26641 taatggatgc gattggtcaa ggcatcaaca tcgatattcc aggtaatggt atttgtttta
    26701 gtattgatgt agacaaagtc attggtatca ctaagattca tcaatatcga gaaattatcg
    26761 gaatgcaaga acttttagat gaaaataacg atagagaaaa gtctgaatca tagacaagcg
    26821 tttgaagagg aaataaacac aaacaagaag gcggatgagc gttatttccg cttttttgtt
    26881 tgtatttttc tcgtgaatac ttatgaaata gtaaattttt atgaaattct actctaagca
    26941 tcagactttc ttttcaaaat atgctatttt aaaaatgatg aaaaaagggg gagaaaaatg
    27001 aattttttag ttgttttaaa agatactctg attgagcgat ctacatttct atatgttcaa
    27061 ttgcaggaaa ataaaacatt gctgcatttt tcaccagagt tccatctaga ggggctaaca
    27121 ttaggagaaa tctatcaagc agaagggctg tcggctgttt tgcacttttt taaggtagaa
    27181 ttggaattac cagtaaaaga ttatatcgag ctttctcttg aatcttggga tcgagcagtg
    27241 gttgaactgt ttccagaagg tttaatgatc gatacaaacg atgggaagat ccatctgact
    27301 gcagcagaac tgcaaaaaca aatgcgttat caaatagatg acgaaaattc tttttctatt
    27361 tttagacgtc agcaaaagat tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat
    27421 ctgatcaaaa cgacacagct gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa
    27481 tttcctcaat tgattacgat gatccatcgt tttatacgta tgcaacagct accatctaag
    27541 aagctgtttc ttccattgac ggacacattt cgagtagtca acagagaaga taaaaagaaa
    27601 gtgattgtga ttgactttac cagaaatcgg aatgtccttc accaagttgc aatggaaaag
    27661 gccaattaag gctttttctt tttggatttt tttgtcataa taaatattag atgatggagg
    27721 agataatatg atgcaaacat ggttagaaag gctaatacga tttcagcaga actatcacaa
    27781 acttatgcag ggaagatatg cgaaatttga tcagttgaat cgcggtctat tgatcgctgc
    27841 gcttgtcttt agtttgctaa gtcgttggtt gccctataga atcggacaat tgttgttcat
    27901 cgtctttttc ggatggatga tatttcgttt cctatcaaaa aaaatctacc ccagactaaa
    27961 tgagaatcaa cgttatttga aatatcttga tagagtcaaa gaaaaatatc atcagataaa
    28021 aaacaaaaag caagtcaaaa tggatcgaaa aagttatact ttttttgagt gcccgaattg
    28081 tcatcaaaag cagcgtgcac ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg
    28141 tggtataaaa ttcgaaacga atgtttaagg gagaaaagaa tgaaattagt attattacgt
    28201 catggcgaaa gcgaagcaaa ttttgaaaat tactggacag gatggctaga tgtcgcctta
    28261 acagaaaaag gaaaagaaca agcagcagtt gccggtcaaa aaatgcttga agccggactt
    28321 caattcgatg ccgtttatac ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca
    28381 ctagaagaaa tgggtcagtt atggcttcct ataataaaaa catggcgtct aaatgaacgg
    28441 cattatgggg atcttgtggg taaaaacaaa gataagatga aagaaatata cggaaaagat
    28501 caagtaaaaa catggcgcag aggatatacg gaagttccgc cgttaaccgc agaaaataga
    28561 tttgaccgca gatatgatca cttggatccg catttgatcc catacggaga aagtctggaa
    28621 atgacagtga atcggatcat tcccttatgg caagatcatc tagcaccaaa actaaaagat
    28681 cggcaagatt tactagttgt gggtcatggt aatagcatcc gagcactgac aaaatattta
    28741 gaagatgttc ctgaagatca aatggatacg atcgatatcc caaatgcaca accaatccag
    28801 tatacattag cagatgattt gacaattctg gacaaaaaaa tactttaaag aacaccttta
    28861 tctaaaagtg agagatagaa aaaagaagca aaggaatgga acaaaaggaa catgaaaaat
    28921 tatatatggg actttgatgg tacattgttt gatacgtatc ccgcaatggt agatggtgca
    28981 tggcaagcac taaaagattt tggtatcagt atggacaaaa aagaaatcta cttcaaaatg
    29041 aaaaagtatt ctactagtta tttgatcaat gaatcaaact tgaatgccag agagtttaat
    29101 gaattatttc accgatatga aaaagaaagt acggaagttt cccgtccatt tccagaaacg
    29161 aaacaagtac tggaaatgct aaaagacaac ggcggtcgtc atttcatcct gacccaccgt
    29221 ttgacagaat caacatgggg cctgttaaaa gaacatcgat tggcacatct gattgaggaa
    29281 gtagtaggga tcgatcagga ctttcctcgt aaacctgatc cagcatcttt aaattatttg
    29341 atcgatactt ttcatttaga aagaacagac acgatgatga tcggcgatcg tcgtttagat
    29401 atcgaagcag ggaaaaatgc aggagtcgct acttgtcttt acgacatcga tcattttctg
    29461 ggtgagatcc cggcagatta tgtggtagga aatctgaatg aaatccttac cttgcagagc
    29521 atcattaaaa agtagtatcc cctttcaaag gagatatgtt ataattttca taaaggagtg
    29581 ataaaattat atggaaatca gaagacaaaa agaagttgta gaagcatttc attatgatat
    29641 gagattgccg aaccaagaag tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga
    29701 aacgatcgaa aactatccga aagaacatag catcgtagga gcacgcttag agttccgttt
    29761 agtgtttgat acatatgttt tatctggaag tgtgagtcag atcaatcata tcctcaatcg
    29821 gaaaattgaa ggtcaaaatg atgtcacaca agaagaagtg gacgagctag tagcaccgtt
    29881 attcgatatc gtacgccgaa tgtcttatga ggtgacagag atcgccttag atcagcctgg
    29941 ggtaaaattg aattttcaat cagaaaaaca agaatagaca gtaaaaacat acctgcgata
    30001 gattaaagct atcacaggta tgttttttta ttactaattg taagcaaaaa gaatgtcatt
    30061 tcgataaaaa tcgtctaaaa tataaaagtc actgaaaaaa attttttcag gaggatggtt
    30121 gaagaatcat tctcgggaac ctttacttcc ttcgatggac ttgtcattaa taatatgagg
    30181 aggagaaaaa tgacaacact catcacagtt ttagtggttc tagtttttgc ggcattatta
    30241 tatgtgttct atcgtatgca aaagcgccat acaaaatttt caacacgcgt ctttacagca
    30301 ttaggagttg gtatcgtctt tggcggtctg cttcaattga tctttggtgt agacgacaaa
    30361 gtcgttactc aatcaatgga atggatcggg attgtaggtc aaggatatgt atcattcttg
    30421 caaatgctag ttatgccttt agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa
    30481 gtttctgaaa agctagggaa aattagtgcg actgtattga cttctttact tggaacaaca
    30541 gctattgcag cattgatcgg aattggttca acaatgctgt tcggactcca aggagctaaa
    30601 ttcacacaag gagctgcaga aacagcacga atctctgaac tggcaacacg tcaggaaaca
    30661 gttgaaaact tatcgattcc acagcagatt ttatccttta tccctaaaaa cgtttttgct
    30721 gatttagcag gtagtcgtgc aacaagtacg attggagtcg ttattttcgc ggcatttgtc
    30781 ggaatcgctt atctaggcgt gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat
    30841 ttagtcgaca gtctgtataa aatcattatg cggatcgtga cgcttgtttt gcgtttgaca
    30901 ccttatggag tatttgcttt gatgacaaac gttttggcaa caagtgattt tgcagctatc
    30961 gtcaatttag gtaaatttgt cgttgcttct tacgtagcat tgcttgctgt tttgattgtc
    31021 cactcactga ttttgattgg tgcaaaagtc aatccaatca actatttcaa aaaagccttt
    31081 ccggtattga gctttgcgtt tacttcacgt tcaagtgctg gagctttgcc attgaatatc
    31141 gagactcaga caaaagcttt aggagtagat gatgcaacag ctaatttctc agcaagtttt
    31201 ggtttgtcta ttggacaaaa cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc
    31261 gtcgcaccag cagctggagt gaatgtcttt agtttggaat ttattttgat gcttgtggca
    31321 attgtaacaa tcagttcttt tggtgtagct ggagtcggtg ggggcgcaac atttgcttca
    31381 ctgatcgttt taggggcaat gaacttgcca gtagcaatcg ttgggttagt tatctctgtt
    31441 gaaccattaa tcgatatggc acgtacagca gtaaacgtca gcggtagcat ggtagctggt
    31501 atcgtgacaa gtgcacgcat caaagattta gatcgcgatg tcatcaatga tgattctaaa
    31561 gtgatcgatg tgaatgcata gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa
    31621 ataagaagaa gtttcctaaa aatagtttct caaatttttg tgaaatttcg gcttatttcc
    31681 gagaagctac ttctgaaaca ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag
    31741 taataagaac agccaaacaa aaatagttcc tcatatttta cgtttggttg agcttattac
    31801 cgcagatgca agcttttgaa caccgtttta tttgtctcac acccttttcc ttcctacttg
    31861 taaccttgct cccgtgttag aaacatgtta cactaaatat gtgaaattat taacaaggag
    31921 cgtcgttatg cgagaaaaaa cagatacact gccaaaagca actgcgaaaa gattaccttt
    31981 atatttacgc tacttgaaga tgctggatga ctcaggtata agtagaatca aatcaaatga
    32041 gttcagcgaa atcacccaaa ttccttctgc aacgatccgc agagacttct cgcaattagg
    32101 cgagctaggc agaagcggat atggctatga tgtccctttt cttatcgatg tattcaataa
    32161 tatccttaat actaaagaag aaaaacgaat cgcattagta ggatatggaa atcttggcaa
    32221 agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta aatatcgtgt gcgtctttga
    32281 taatgatcca gcactgatca atcgagtaat tgatggagaa atgatctatc cgattgatcg
    32341 tttcgcggag atagccaaag caaaaaatgt gacagtagca atctctacgg taccaagtaa
    32401 atattcacaa tctgctattg atgaaattgt caaaggaaat gtaaccgcta tacttaattt
    32461 tgcgcctgac agagtaactg tcccagctta tgtcaatgtt caatacattg atttgacaac
    32521 cgagttgcag acgcttattt actttgatga gaattactca gaagtttttt cttaggaaaa
    32581 tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat tctttcaaaa attttttagt
    32641 actaaatgaa gtggtttttt cacgaccact tgttgactaa aaaaaaaaac cattgtagaa
    32701 tgaagcataa cgctgaacaa gaggagtaac tggcacattc attcttagag agagaactat
    32761 ctgctgaaag gttctcaatg acaaccagtg aaggtagctt gggagtttct gtcttgaatg
    32821 gagtagagat agacgagtcg tgatcgttaa ctcacttgag aggcaataat gattgattat
    32881 tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa tacgaaagta ttttaggacg
    32941 ttttttagtt taaaggagga aaacagcatg acggaagaaa ataacctatc aacaaaatat
    33001 aatccgcaag aagttgaagc tggacgttat cagcattggt tagaacaaga tttgtttaaa
    33061 ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa tcccgccccc aaatgttact
    33121 ggaaaacttc atttagggca tgcttgggat acgaccttgc aagatatgct gattcgtcaa
    33181 aagagaatgc aaggatatga tactttatgg ctgcctggta tggaccatgc aggaatcgcg
    33241 acacaagcaa aagtagaagc gaagcttaga gagcaaggga tttctcgtta tgatctaggt
    33301 cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag agtatgcgag ccacatccgc
    33361 gaacagtggg ctaaaatggg cttatcttta gattacagta gggaacgttt tacattagat
    33421 gatggattat ctgaagcagt acgtaaagtt tttgttacct tatacgaaaa aggtttgatt
    33481 tatcgtggtg aatatatcat caactgggat cctcaagcac gaacagctct atctgatatc
    33541 gaagttattc ataaagatat cgaaggcgcc ttttatcata tgagctatga actagcagat
    33601 ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa cgatgttggg agatacggcg
    33661 attgctgttc atccagaaga cgagcgttat caagcgctca tcggcaaaaa agtgattcta
    33721 cctttagtca ataaacaaat cccaatcatt gcagatgaat atgtagatat ggaattcggg
    33781 acaggtgtag taaaaatcac tccagcacat gatccaaacg actttgaggt aggtaatcgt
    33841 catgatcttc cacgcgtaaa tgttatggat gatgatggta cgatgaatga actagcagga
    33901 aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa tcgtgcaaga tctgaaagaa
    33961 atcggtcgat tgattaaaat cgatacgatg atccacagtg tgggtcattc cgagcggaca
    34021 ggtgtcgttg ttgaacctcg gctgtctact caatggtttg taaaaatggc gccgttagcg
    34081 aaaaaagcca ttgataacca agaaacagat caagcagtgg aattcttccc tccaagattc
    34141 aatcaaacgt ttttacgttg gatggaaaat gtccatgatt gggtaatctc acgacaatta
    34201 tggtggggac atcaaattcc tgcatggtat cacaaagaaa caggtgaaat gtatgtaggg
    34261 atggaagcac cagcggatag tgaaaactgg gtacaagatc ctgatgtttt agacacttgg
    34321 tttagttcag ctttgtggcc tttctcaaca atgggctggc cagatacgga aagcgaagat
    34381 tataagcgat acttccctac tagcacttta gtaaccggtt atgacatcat tttcttctgg
    34441 gtaagcagaa tgattttcca aagcttggaa tttactggcg aacgtccatt tgagaatgtc
    34501 ttgattcatg gattgattcg tgacgaacaa gggcggaaga tgagtaaatc attaggtaat
    34561 ggaatcgatc cgatggaagt aatcgaaaag tatggagcag atgctctccg ctggttctta
    34621 tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct atgaaaaaat ggatgcttct
    34681 tggaacttta tcaacaaaat ctggaatgct tcacgtttcg ttatcatgaa tatcgaagga
    34741 atgaccgtag aagagatcga tttatctgga gaaaaatcag tggctgaccg gtggatcctt
    34801 actcgtttga atgaaacgat cgaacgagta actgagctat ttgatcgttt tgaatttggt
    34861 gaagcaggtc gacaactgta taactttatc tgggatgatt tttgtgactg gtacatcgaa
    34921 atgagtaaag aagtacttta tggagatgat gctgtttcaa aacaaaccac ccgtagcatc
    34981 ttagtccata cacttgatca gatcctacgt ttattgcatc caatcatgcc attcgttaca
    35041 gaagaaatat gggaacatat tcctcatcag ggaaattctc tagtagtggc agaatatcct
    35101 gttgtacgtc cagagtttga tgatgaaaca gcttctaaag ggatggaagt attgaaagaa
    35161 ttgattcgag ccgttcgaaa tattcgttca gaagtgaata caccattgtc taaaccaatt
    35221 acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat ttttagttga aaatacaagc
    35281 tatatcgaac gcttctgtaa tcccgaagag ttgacgatat caagtgagat cactgcgcca
    35341 gatctagcga tgtcagctgt tctgacaggt gcagaaatct tcttgccgtt agcaggattg
    35401 atcaatattg aagaagaaat caaacgatta gaaaaagaac tggaaaagtg gacaagcgaa
    35461 gtaaaacgtg tccaaggaaa actttctaat gaacgttttg tttctaacgc gccggaagaa
    35521 gtagtagagg cagagcgtgc aaaagaaaaa gattatttag agaagcaaac ggctgtcaaa
    35581 gaacgaatcg aaagtttgcg tactattcaa taagaataga aaaagagagt gtgaaatcag
    35641 ctagctatta ctaaaagcta gctgattgaa catcgttaca ggaaagagtt tgagcctcac
    35701 tcttagcttc gattcataag gagatgtgac ataaattttg tcgcatctct tttttagtct
    35761 actcttatta tgtaggagaa ggagggaggt ggatcatgta tcaagagtca ataaagtagt
    35821 tgataaaatc gttcctctct ctgtttcttt tctctttgtc atacttttat ttttcttatg
    35881 tgttctattt gttataataa aagagaaatg ggggaattgt tgatgacgat tgaagaagca
    35941 atagaatgga tccatagtcg attaccattt ggttcacgtc ctggccttga ccgagtcgaa
    36001 gctttattag aagtggtagg gcatccagag agaaaagtaa aaacgatcca tatcgctgga
    36061 acaaacggaa aaggttctac tgtagcttat ttgagaagcc tattagaaga ggctgggctg
    36121 acagtaggta catttacttc accttatatc gaaacgttca atgaacggat cgcaatcaat
    36181 ggcaaaagca tttcagacaa tgatttgatc cagcttgtaa aaaaatatca gccactgatc
    36241 aaacaacttg atcaatttga agcagtagct ggtattactg aattcgagac attaacagca
    36301 atggcatttg attatttttt gatgaagaaa gtggacgtag cgattatcga agttggctta
    36361 ggcggcttat tggattctac caatgtagct gtaccaatgc tgacgggaat aacaacgatt
    36421 ggacttgatc ataccgaaat acttggagat acaattgaag aaatcgctgc acaaaaagct
    36481 ggaatcatca aacaaggcgt tccagtagta acaggaaata ttgccccaga agcttttact
    36541 gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt atgtatacgg ggaaaattat
    36601 aaaataaact atctgcatcc tgatgagatg tggggagaag tatttgaatt ttttaatgaa
    36661 ataggaaaga tccctcaatt aaccactccg cttttagggc aacatcaagc tgaaaatgca
    36721 ggagtagcca tcgaattgta ttttgtttac tgtcaattgg aaaagctgcc tttccgagaa
    36781 aaagatgttt tgaagggatt gaagaaatct cgatggccag gaagaatgga gcgtttgagt
    36841 aacgagccat tagttgtttt agatggtgcc cataatacgc atgcaatgaa ccggcttgtt
    36901 gagaatttga aaaaagaatt caaaggatat caaatcaaca ttttgttttc cgccttacga
    36961 actaaaaatg ttagagaaat gctagaacga ttgcagcaag taccaaatgt ccatatttat
    37021 ttgacgacct ttgaacatcc aaaagccatc gaattaagtg aagtatcaga tttagcaagt
    37081 gagagagtat caatcgtttc attgtggcaa ttcggattag cagatatatt ggaaaagatg
    37141 acaaatgaag acgtattagt tgtgaccggt tcactttatt tcatttcaga agttcgccat
    37201 ttattaatac aattaggagg agatcatgaa gtataatgca gtgatttttg acatggatgg
    37261 tttgctgttc gacacagagt tagtgtatta cgaagccaca caagtagtag cagacaagat
    37321 gggttttcct tatgataaag acctgtattt aaaattctta ggtgtttcag atgaagaagt
    37381 atgggcgaat tatcatatga ttttctcgga atttggcaaa acaagtgtac aaagattcat
    37441 agatgattca taccaggaaa cgcttgaacg tttttcgcaa ggagcggtac aaatgaagcc
    37501 cggtgtgttg gaactcctct cttttttaga agaacatcag attcccaaag ttgtcgcttc
    37561 cagtaaccaa cgagcagtca ttgaactttt attgaagaag aacaatctgt ttgaacgatt
    37621 tgagacgatt gtttctgcag aaaatgttca acgagcaaaa ccagacccag aaattttctt
    37681 gttagctaat cagaagctag gtacagcgaa gaaagaaaca ttgattttag aagattcaca
    37741 aaatggtatt ttagcagctt ctgcggctga gatcccggta gtcatggtac cagatctgct
    37801 tccaccatcc caagaattga tcaaaaaaac agctggggtt gtttcttctt tacatgaaat
    37861 ccctgcattg ttaaaataaa aatccatctc ttttcgtatc ttattttaga taagatagaa
    37921 aggagatgtt tttttgggaa aacgattgat caaagaagta cctactagct ctttaccaag
    37981 ggaacgtatg gaaatttatg gcgcggaagc tttatcggat caggagcttt tggcaatctt
    38041 actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt gctggaaatc tgttaaaaac
    38101 gtttggagga ctggcctctt tacgacaggc tactttgcat gagttagaag agattcgagg
    38161 gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc gaattaggtc gacgaattca
    38221 gacggatcat gaaagagcgg ctccaccggt tcgtaccagc tacgaattgg cccagcaatt
    38281 gattctggaa atgaaagatc acaaacaaga acatttggtt tgtatttatt tggacacgaa
    38341 gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg ctgaatcagt cagtcgctca
    38401 tcctagagaa attttccatt atgctgtacg ttattgtgca gcaaggatcg ttttagcgca
    38461 taatcatcct tccggaatat ataagttagt acacataaat tatataagga aatttactgt
    38521 tatattgcca tctggaaata ctattatgtt atcgataatt gaacgccata atgcattttt
    38581 ttcttcttcc gaagattctt tataaatttc atcgaattct tttgatagaa taaattcgac
    38641 attttttata tctattttag ctggctctac ttcaatttca tttaattctt tcattaaagc
    38701 ttctcgatct tttttgtatt cctctaagtc tataacttca ttcaagtagg ctttcttcaa
    38761 tctatctaat ttattctcaa tatttttctt actatttttc tttttaataa tctttggttg
    38821 tttttttgaa tagtttattt gtatttgttc aaatttttgt ttagtagctt caaaaatata
    38881 acgttctaaa tttttttcgt aaaaagcgcg cttatttgtg caacgtaatt tattatttct
    38941 tcgttttaca catctataac aagtcctttc gtatatagat tctgtaccgt cttttttctt
    39001 atatcttgct ttaatagttt gaccagctac tgatccctga cagtcagaac attttaataa
    39061 acctttaaaa atgtaatcac gttttgcatt tgtttttaaa tttttaggta aggctttttg
    39121 aacagcataa aaaagttttt tatcaataat aggaggacag taatttttat tatttcttaa
    39181 ttcaccaatg tattttttgt tcgtcaacat atttttaaca ctttgataat ctctaacgat
    39241 tccatactga gagcctaaat aataaacagt ttttcttaaa ctaccactag aaagaaagta
    39301 gttgaatata tctatcacga taggagcatc atcatttatt actaatttct tattttctat
    39361 agagtagcct aacggagttt tcccgcttac tacttcacct tgtgcaattt tatttgccat
    39421 aacagctttg atgcgttctg aatcaatttg ggcttccaat tcagcaaaac tcattacttg
    39481 tgcaatgaaa gttctgccat acgctgtagt tgtatcataa tattgttgag aaacagcatt
    39541 ccaagaaaca ttgtgttttt ctaaaatttc ttgagtgttt aaataatgtc gtagatttct
    39601 aaaccatcta tctaattttg taaataaaat tagatctatt ttattttctt ttacatcttg
    39661 taatagtttt tgaaattcat cacgatttat tttttgacca gaaataccac cgtctacata
    39721 ttcattaact actttcaaat ccttgtgttg ttcgatatat tcatagaggg tttctttttg
    39781 ttctctaaga ctatctccat gtttggcttg ttgttcagtt gatactctca tatataatgc
    39841 tactcgtttc atgttatctc ctttcgaatg tatgttctta tcgttgtaaa agaaaagccc
    39901 gaaggctaat ctttttacta cacatctgtt aattctaatg atcttctgta ttgttcagct
    39961 tgagctagtt tagtaaaaga tacagttccg ttataatttt ctaaaacaac tttttcaatt
    40021 tcatcaagag atactttaaa gaattctttt cgttgattaa ctttattgac ttcgtttttt
    40081 ctaaaatgcg aatgtagttg attttctaat gttggtgcat cttctgaaaa tatcattgca
    40141 tgaacatcaa attcaaaagg tacagaagcg ctactaagtt ccttaattct atccataggt
    40201 tctaaccgtc tagtcattcc tattttatag acatcttcac cgaaagatcc gatattactg
    40261 atgacataaa catagccagc tctagtatta agttctcttt gcaatacatc ttctttgaca
    40321 gtttctagtt ctcttaattt ctcttccagt tctttaattt tatcagcata gagttctttt
    40381 tcaacatcag cactagcttt ttgcatgtat gccattaact tagttatctc tgagttaaac
    40441 tgtcgttctt ctttatcaat gcgtttcttt tcacgttcta tttcacgtct taccttttct
    40501 tcttcaatca tttgttcttt tattgctttt tgctgttctt tttcatcgtt aactttaact
    40561 atgtatgaat agttgagttc taattcttga agttttaatt caagtaattc ttttttgaat
    40621 tctgcttcat cgtttttaaa aagcttgttt attttatcaa aagttcttat aattttttct
    40681 ctagttgaat ctacgttaga aatagtaagt ttattgagaa gtccagttat ttctgattct
    40741 aatggtataa gcattttctt tttaatatta ttagcttctc tattattcaa ataagaagga
    40801 atattaattg caccgtctaa aataaaatcc ttttctttaa tctttaataa agaaagtttg
    40861 ttttttattt cggatgaaga agtttcatct atattcggaa taattactga tttatagata
    40921 gtgaccagag atgtgtcata tttttgatta atttcatcgt ctaaccgctg gctttcagca
    40981 ttaagaatat caattttgtt ttctagcgtt gacaagtttt tattcaattt acttatgttt
    41041 ttctcttttt cagatataaa cgtttcccta gcatttaatt cttgagaata ttcctttacc
    41101 ttactctcta aggcagaagt tttatctttt aattttttag cttcagataa tttgctacta
    41161 cccataatta ttaaaactat tccaactaaa aacaatagta ctcccattca aaacgcctcg
    41221 cttcttttat aaaataattg tcaaaatcat ataaaccctt accgaagttg acggctccgg
    41281 catgggaatt tttttaaaat acaacttttc cgataattct aatctgttct ggcgatacaa
    41341 ttctatcttc gtataagtcg tttatagaac gtaaaatgat tgtttgattt tcgtaatctt
    41401 tatatatctc tttacatgta actccgtcac cacctaattc cacaatagca atttccccat
    41461 cttctacgtc cggttgttct ttaacgaata cgaattgtcc attcctgata acaggttcca
    41521 ttgaatcgcc tataataggt actgcgaagt cagcagcact tggaactctc tcaaattctt
    41581 cctcttcgac aaccgtatca ccgtaagtaa gttctgttgg attggcagcc gaagccgaat
    41641 tgtgaacagt tgggattttt tcttttttag gaaaatctag gacattttct tgtttttgtt
    41701 cttctaatcg attacttgca aattcatagg tagcaacttt attttcgtcg ttaagctgat
    41761 tatatatagg aaggatatta tatatctgat tagatataga taaatcaata ttcatcaaat
    41821 catctatatc aacattgaat atcttagcta tctcatttaa ggtttttaat tttggtgtat
    41881 attttccttt ttcccattcg cttattgagg atgaactttt tcttcctagt ttttcggcta
    41941 gttcttgttg atcaatatta tacttttctc taagaaactt tagattttca gcgaacatag
    42001 taacacctcc ttgagttaat tgtaacaccg tttcggaaaa aatgtaacag gtttcttaga
    42061 aaatatttta gtgtttttaa agaagtattt acgcaaaata gaaatcgatt tcaggaaaac
    42121 cgaaatatgc acttgacttc ggaaaaaccg aagtatataa tttagtcaga aagttaaaga
    42181 gaggaggcga ataaaatatg gataccgcga agaaagaaaa atatacattg agacaattaa
    42241 gagctttaaa gggttattca aaagaagaac tgtcaaggaa aagcaaagtg acatcaagaa
    42301 caatatttat ttacgaaaac gatgtagata aaatgaggaa cggaaagtac gccactctag
    42361 aaaaaattgc taaggcattg ggtgttagag tttccgatat ttttttagac cctgattcgg
    42421 aaaaaccgaa atcaataaca ggtgaaacaa aacaagtcag ctaggaggtg tgaatagtgg
    42481 aagagaagaa tataaaagta ccgactcttg aagaatatac aaaaattgtt gtcgaaacag
    42541 acgagcagaa cccgaaaaca atcgcagtta ttacggcgaa tgatattgaa tcatgcgaag
    42601 gttttcgagt tcgaatgacc cctagatatg attaatgttc tttatcacgt ggaggtatag
    42661 gatcgtttcc aaaactatct tttgattgta tttgtccatt ctttttttgg acaataaact
    42721 ctgattgctg ttttttagaa atctttttac catactcaac agcatctgat ttattatcaa
    42781 atagttttgt atcttcggaa ttaccttcac cacgtacagc ccatttgcca tttctaggag
    42841 atacccattg atttttaccc ataacaattc acctcgcttt caagttaatt ctattaagag
    42901 gtgaaaagaa aacaaactga tggaggtgag attgtgagta acaagccaaa aatcactttg
    42961 aatggcacac ctaatcttca aagattagca gatgaaatgg ctagaatacg ttctagacat
    43021 tggggagtta ctgtggtggc aaccataatt gaacaaaaag aaaagcccac acagccggca
    43081 agcaagtagt gggcaccata gaacaataat attatatcac acactcgcca cttccgaaaa
    43141 gaggagttgc tgctctcctt tcaagtttgc caaagtccac gtttcttcat tttggcaact
    43201 cctcttcttg gaggtggtga cggcgagaga cgaaggaggg atgacatgaa ggaagaatca
    43261 aaaataggga ttttgaatga gaacaattac acaagattaa ttattgaaac agaagaaggg
    43321 gaaaaagtag cagaagttac attgaccgat gcaactcctg ctgacggtta tcgtattcga
    43381 ttaactccta aatatgatta accttttggt gggtgtggat cgtttccggg actatcacgg
    43441 ctttgaattt ttccatctct gccatgaata aatagctcag atccttgatt tttagaaatc
    43501 tctctagcga tttttactgc ttctgatttt gtattagtat gtgcagtcgc tttagaattt
    43561 ccagcacctt tgacattcca accaccattg gaattaggta caacatgttg attttttccc
    43621 atagttttca cctccatttc tatagaaata ttttatcaaa tagaaatgga gaaattcaat
    43681 aaacgaaagg agggatcaaa tgacattaca agagcatcaa cacttgatgc gaaaactaaa
    43741 tcaggaatac cacgaggaaa tgaaacctag attgctaggg gaacaatggc gtgaacgcca
    43801 gaagaagtgg ttagaaataa aaaagtgact cagccgtcca aagcaatgag tcacaaacca
    43861 aaaattatac ctaaggagat tttatcacat gaataagaaa attgaaaatc ttatcgaaga
    43921 attgaaacgt gaatgccaaa agcaaggtgt ttctattatt tgtacagccc aaaaagaagg
    43981 ggaacttaaa agtctcgttt atggtgagac aacagaaatt ttactttgtc ttgctatgca
    44041 agaagaacat ttagatgaaa atctcccatt gtcagcacat attatgcgta gaattgctgt
    44101 ggatgcttac gaacaagcga aaaatgaaga agaaaatcaa ccttcaaatc atacttttgt
    44161 tattaataac aaggaagatt tagctgatgt gatgactcgt attctcaagg gggaatttca
    44221 atgatgaatg aaaaaacaat tgatctactc attattttat ttttcacgtt agtgattgta
    44281 gcgaaaagtt atgtaatctt tggcttgtta ttcgcaatta ccatgcttgt gctttgcgtg
    44341 ttagcaagca gaaaagaggt taaacatgaa cgagttagca aatcttgaca actatttaac
    44401 tgatcctgat tatgctaaac caccatatga agcgccaatt gatgaggagg atgaagatga
    44461 gtaaatctac cttagaaatg agccatcaag aatggctcga agaccgcaaa agaggtattg
    44521 gtggctcaga tgttgcaacc gtacttggat taaacaaata caaatctcct tatcaattgt
    44581 ggcttgagaa aacgggtcaa attgaattga aagatttaga aagcgaacca gcttattggg
    44641 gtaatgtttt agaagaagtg gttgctaaag agtttcagga acgtacaggc aaaaaagtac
    44701 gcagaagaaa ccaagtattt gaacatccat tacatccatt tttaagagca aatattgatc
    44761 gggaagtagt gggagaaaat gccattctgg aatgtaaaac agccaatcaa tttctcggaa
    44821 aagagtggga aggtgaagag gtaccgctta gctatctctg tcaagttcag cattacatga
    44881 acgttttaaa caaagactat tgttatatcg ctgttttgat cggtggacaa aaattcattt
    44941 ggaagcggat tgaacgagat caagagctga tcgatacaat cactgaacaa ttagtagagt
    45001 tttgggaaac gaacgttctt ggaggtatcg agcctgtaat tgacggaagt ggagcgactg
    45061 ctgacttctt aaaagaaaaa tatgcagatg tagaagaaaa tcaaacagct ctaccatcac
    45121 gttttgatga acttatcgag caaaaaagag aactcaaacg gacgaaaaaa gaaattgaat
    45181 cagctatccg tcaagtagac aacgagatta tcagcgagct aggtaaacgt gaagccagta
    45241 tcggtatcac tcaaaaaaac atcatcagtt ggaaatttgt ccgtacgaga cgtatgaact
    45301 cgaagaaact agcagagaaa tatccagatg tcgcaaatga tgaagagatt tataacgtta
    45361 ctgaatcaag aaggctaacc gaaaagggga tcaaataata tggcaacaaa tgaatcgtta
    45421 aaaaatcaat tggcagaaaa gccacagaaa caagttgcac caggacagtt agggcttaaa
    45481 gctctaatga atacaccaac aatgagaaag aaatttgaag aagtacttca tgacaatgct
    45541 aatgctttta tgtcgaatgt tatgactctt gtatctaatg acagttatct tgcagatagt
    45601 gaaccgatgt ctatcatgag tggtgcgtta actgctgcaa cattaaatct tgggctagat
    45661 aagaatttag gttatgcata tttagttcca ttcaatagta aaaacaagca aacaggaaaa
    45721 tgggaaaaga aagctcaatt catgcttggc tataaaggat atatccaatt agcccaacga
    45781 tcaggtaaat acaaagcatt aaatgtgatc gaagtttacg aaggagaact aaaaagctgg
    45841 aaccgactga cagaagagtt tgagtttgat ccaaatggta gaacgtctga tgaagtcatt
    45901 ggatatgttg gctatttcga gttactgaat ggattcaaaa aaactgtcta ttggaccaaa
    45961 caagaaatcg aagctcatcg gattgctaac aataaagatc gcgataagac aaagttaagt
    46021 ggtgtgtggg catctgatta caatgcaatg gcacgaaaaa ctgttttgag aaatcttctt
    46081 tctaaatggg gaatcttgtc catcgaaatg caagaagcca ccacatcgga tgagagagtc
    46141 caaagggttc aagaagacgg cagcattatt gctgaaacag aagttgaaga agatattcct
    46201 gaaagaaaag aagcagaggt tatatctgaa gaaaatgaag atgtacaaac tggattattt
    46261 gatgcatcta atccgccgtt aaacaaataa tgagggagct ttctcccttg cttttctaga
    46321 ggagaaatac gaatgaacac aggatatgtc aaattacatc gaaaagtgat gaattcattc
    46381 gtttggacca atccttatat gtataaatta tggagcttgt gtttgatgaa agcgagtcat
    46441 gagaatcgca agatactttt taatggaaaa gagatacaag tgaacagcgg agaattcgtc
    46501 acagggcgcg atgctatcac atctgagatg aacgaaggcg tgaaacgtga acatcaagtg
    46561 aacagcggtt ctgtatggag atggctaaaa caatttgaaa aaaacggaat gttgaacatc
    46621 aaatcaacta cgaaatacag cgttatatca ataaaaaact ggtctttgta ccaaagcagt
    46681 gaacaacaaa tgaacatcaa acgcacaaca agtgaacaac aagtgcacac aaacaagaat
    46741 gaaaagaatt ataagaatga aaagaataat aaaacatcat cgtcgcaacc acgcaaaaag
    46801 cgtgtttacg acaccgactc agtttactac attctcgcgg aggagttatt caaacagatt
    46861 tgtcagaatc aggaaatcaa aaagccgaat ctgcaaagtt gggcagataa cattcggaaa
    46921 atgatcgaga tcgacaagcg gaccgaaaac caggtacggg gaatgattga atggagtcag
    46981 cacaatgtgt tttgggcatc gaatatttta tctgctaaaa aattgcgaga aaaatacgac
    47041 acaatggcag cgcaagcaaa tcgtgattat aaaacaaaac aaactaaaac gcttgaatac
    47101 gagaaattta gcacagatga gttgcctatt tgagaggagg cgtaggcatg gaaactgttg
    47161 gggaaatcat ggaaaagctg atacagaaag tgcttgtcca acgtggcgaa tgtcctgaat
    47221 gcggacagcc tttgtatgga tggcgtacga agaaccctga tggctcagaa cgttgtaagc
    47281 caacctgtat gcaatgtggg tataaagcat tgcgagttca ggaagactta caaacagaac
    47341 gaatttacaa cgagagcctg aaagcaagag caatcaattt tttcaaaggt ggttctgttg
    47401 tgcctaatca agcgttgttt gattgcacat tgcagaatta tcaaattgtc gatcaagaaa
    47461 caagacaagc ggttgaagta accaaacgct ttgttaattc agtcttgtta ggaaatccaa
    47521 gtcaccttgt tttaactgga aaacaaggaa caggtaaaag tcacctagcg atggcagcgg
    47581 cttgggaagt cttgaagcga tcaaactacg ataaaaaaat cttatttatc gggttacagg
    47641 aaatgctgga tcaaatcaaa ttttcgtaca acaatcctga actcagaaaa acgattgagg
    47701 gatcgttgat tgcagatatc aaaacagcgg atttagtcat cattgatgat atcggttcag
    47761 aactaggaaa agatgcatca gatagtcgag cgtttggcat aaacacgcta aattcgttct
    47821 tggatgcacg acagaaccta gcaacgatta tcacaacgaa cttgcttggt gaagaactga
    47881 aaaaagctta cggtacgaga acaatatcaa gaatgtttgt caactctgat ggatttacga
    47941 tggtattttc tcaaacagca gacaagcgca taaaaccagt gaaaggtagt atcgcatgaa
    48001 taaataccgt aataaaaaaa ctgttcatcg aggtatcaag tttgattcta tcgcggaagc
    48061 agagtattac gatctagcct tgtggcaagc tgaagcgaat ggttggaaag tgaagctcca
    48121 ggaaaggttt gagctgatgc cgaagtttga actagagggc aagaagtatc gcaagatcga
    48181 gtatattccc gacttcacat tttacaaaag cggcaagctg gtcaaagtcg tagatgttaa
    48241 ggggatgcag acaaaagact ttaagatcaa ggcaaagttg ttctgtcatc aatatcaagt
    48301 gccgttgata ttagccaaaa aatatcggaa tacgttcaag gaagagcgtt tttaacgagg
    48361 tggtccatca tgacaacaga agaagtgatt caaatgcgaa ttcgaagcct tcggcgtgag
    48421 attgacgatc tggaacgaac aaaggcagtg atggtcaatg aaacggctag aaaggcaatc
    48481 gatttgcaca tagagaattt aagaagggaa atccgtagat tggaggaatg agcgtggata
    48541 aggaagcggc ttggcgaaaa ttaatgttgc tgattcaaga tgagaactgg caagaagatg
    48601 aagcagtggt tgctgaagtt cagcgtctag gaaagattgc taacggacgt atacgaaaaa
    48661 agccagacaa aagaaaacag cgcaaaggga aaatcgtcgt tgttttacac gaaggcaaaa
    48721 ttttgatgca aggaacagct agtgagctgt ctgcagaaac tggatatacg cgtgggacta
    48781 ttcgaacgta cgcttggcga aatcacaccg atcgaaaagg gcacgaatat aagtatttgg
    48841 aggaagaaaa atgaacgaaa acaaattaat caaattgggt gtagcaggag cagtaatcgt
    48901 aggtattgga gttatcggag gatttaagtt cttcgaaaaa atcgataatg gatatgtggg
    48961 tgtgcgctat tcaatgaacg gcggtatcaa agatgaagca ctgacgcaag gtgtgaaatt
    49021 tgtagggatt gacaaagtga tccaatatcc aattcgcttg caaactatcc aatcaaaaaa
    49081 tatttcagta tctacaagcg acggcaaaaa gacaacgatt gatatcaaat atgactacaa
    49141 agttgattca actaaagcag caaaaatgta caaggaattt gggaatatca cttcggaaga
    49201 tatcgaaagt ggatggttaa aatctaagct tcaaaaggtc gctcgtgaag tttatgcgaa
    49261 atatagtctg cttgatgtcc tttcaggaga ttcctctaaa gttgaagctg aggtattaac
    49321 gaactttgct aaatcagttg aatctaaagg gtttgaagtc gaagacgtaa cacttggtgt
    49381 tccagatgtc gataaagaaa cacaaaaatc aatcgatgcg atcattcgag ctggtcaaga
    49441 aaatgaaaaa gcgaagctag acgcagaaac tgcaaaaact caagctgata gtgaagctta
    49501 caagaaaaca aaagctgcag aagcagaggc agaatctaat cgcaaagtcg ccgaatcagt
    49561 aacagacaat ttgattcgtt atgaagaagc tcaagctcgc aaaaagcatg gatgggtaac
    49621 agtaaatgga gcagatactg tagttacgga tgaagcaggc aaataatatg ggattcttta
    49681 tggctaaaat tctcttgttc ttaggtttag taggagcagc atatctcgtg tatgcgctcc
    49741 tttcccaaac tgatgacaaa gaagatgaca acaacgatga aatgaaattt taggaggaga
    49801 aataatggac gaactaatca caaaagtaga gcagtgggct aaagacaagg gactggatca
    49861 agcagatcca aaagcacagt ttttgaaagt agctgaggaa ttcggggaaa tagcttcggc
    49921 gatggcaaga agtaatgatg aactatttaa agatagcgta ggagacgtta tcgtcacgct
    49981 gattatcctt tccatgcaaa aagggacaaa cgtacaagag tgtttagaaa tggcatacaa
    50041 cgaaatcaaa ggacgcacag ggaaaatggt agatggtgta ttcgtgaagt cgagtgattt
    50101 ggaggatgtg aaatgaaata cgaaatacca ctaagtgaag cgggcattca agcaattatc
    50161 aatggtcggg aggttaacat agaacttcct gatggtactg aattagtcat cagacaaagt
    50221 tatttgaaag atatggcagc tccagtatta attgatcgtt ttaacgtgac tgattctgtg
    50281 gtagagaacc acttaaaaga atttcgatca agtatagaca acactttcag attagggagt
    50341 tgattgacaa tgaacaccag acaccgcaga gtagcaaaac taagaaaaca ggaactaaat
    50401 gtactaaaga caaagtttga aaaagaatat ggaatttcag cagaagaaac atataaagtg
    50461 gcaagtcagt ttgttgctga tgcaagtgat gctattcgta agtttgggat ttcgatatta
    50521 aatgatgatc gtaaatggga ggaaatgaga tgaaactaaa agacggattt tacgctagta
    50581 gtcacggcat cggcggttta atgctagata tgccgacaaa gaaccctaaa acacgtaaga
    50641 aaccaaaagt caaagtcggt gacatggttc gctgtgaagc agaagagttc atttatccgt
    50701 ttcgtggata tgtagaacac gtctataatc actcagcgat cattcgtatt gaaaacacaa
    50761 tggaatgcga taagtggtta gcgaaaagca aagagaattt agctgtagcg agattggtgg
    50821 atatggaggt tataaacaat gaagtttaaa atctttgaag aggacactcg ttataaatta
    50881 gaaaaagaat taaacgattt tgcgaaaaac aatgagattc agcatatatc tttagcaact
    50941 tctaagagag gttatgcaaa ttactatgca gctgttgtga gctatgtaag tcgagaagtg
    51001 taactcggca aataaaaaaa gccggatcgc tccgactgat tcaataaatc caacacattt
    51061 attatatcac ataaaggagc ggtttgactt gatgcaattg ttacgagagg tagatttcaa
    51121 acagacaaga tgtaatgcga gagatgtgct gaagaacttt cggcgtttgg agcggatggc
    51181 aggtcgctct ttgatagata ttaagtcgcc gattattacg gatatgccga aggcaccgaa
    51241 gcacggcaat aaggcagaag acgcgatcat tcaaatgatg gatatagaag cggagagaga
    51301 cgcgattcta gtggctttga tggctcttag tctgattagt cgtcagatac tctactacag
    51361 cttctgtgtg ccagatagct tctcaaacta caggattagc cgtgaagtgg gttattcaga
    51421 aagaagcata caaaggatga agtcggaagc tctaatagag tttgcagaag cgtataaaca
    51481 cggaagaata attgcttata aataggggga ccatataatg tggaaagatt atgtatcgtt
    51541 gaaagagttg aaaaaagatc ttgttttcaa aagaattgta gaatggtcag agagtgaatt
    51601 aattttagaa gatggaacca aaatggaagt tgtatgtagc gaatcagatt gctgcgcatg
    51661 ggccgaaggt gaattcaaaa atgtaaagct tgatgcagtg attacagata taaagatttt
    51721 tgataaaggt aaccgtctct ataatggtga cggacatacc tcttatgctg aagtcgttgt
    51781 ttatcataat cgtaatgaga tcgctaaagc agaatgcaca gcgaatgatg ggaatggcgg
    51841 ctactattat agtgtttgtg ctttaaaggt caaagacaaa ctttgtatag tgactgacgc
    51901 ataaaaaaat ggcggttttt tggcggaatg atggcggttt ttagccattt atcagtgata
    51961 ttatggtagt gtcgaaagat tagtgatagg tctgagacaa aataataata aaaggaacat
    52021 cgttttatta ttgtttcaca attaagcttc gatagacagc aacggaaata ttaagaataa
    52081 ggatgtgaat tttaactcct tctaaattgt tcttattatc tatcatccgt tgctgtctat
    52141 tgttacatac agatcgctta ggcggtcttt ttattttggc cgaaaaccta cattttcgat
    52201 ggccgattat tggaggagga atagcatgtt caaactatct gaaatcatta agaagtccga
    52261 cgttgagaaa ttagagatgt taaaaaagaa gttgaagaaa tagcagtctc ttcgtgaggc
    52321 tgttttattt tgctcacaaa aatagaccac tatcgggtaa tagtggtcag gaattaaatg
    52381 aaaaagatgt tagggttgtt agctaagtat aacatcataa cgcttacaaa acaatgcaag
    52441 aaggataaat tattatgaga acttactggt atgtgtcatt aaacaataaa tatccgaagc
    52501 caatgaaagg acagcatagg cgtgtagtca tgtctgttca aatgaaggcg aagtattcga
    52561 ttgtagaaat gatcagagag gcaacgccag tagagattga ttattgcaag ctagtctatt
    52621 gtgggtgcgg ccgttggaaa gaggatcatg tacaaaaaaa tatcagcaag tacatataga
    52681 gaggcggtga ttaaggatgg aaaaaagcga tacgaaagcg aacagataca aacctactgc
    52741 tgcagagaaa aaactcttgg aagtattgat aaatccagat aacttaggca aaccggtgca
    52801 agagttgtgt tcactggcga aaataggtag gaccaaatat tacgacgcca tgagcaaaga
    52861 tggctttgtt gatctagtta acgaaacaac aatggatctg atcaaaggaa aagcttctga
    52921 tgtccttaat gccacttaca agtatgcatt gactgaaaaa ggtcatcagg ataggaaaat
    52981 gattctgact attgctggaa tctatgcaga caaacaagaa ctcaaacatt ctggcggcgt
    53041 cgatatccga aaacagtatg agaaaatgtc cgatgaagaa ttagaagagt tggtgaaacg
    53101 gtatgaaaaa atcaacgact cttaatcgtg ttgagtatgc cgttgttcaa agagagctag
    53161 ccattcgaaa agctaggaaa agtttttggc tattctgcca gcttctttat ccagagtttt
    53221 acaaagatga tcggttgtat ttgaaagatt tatgtcatac gctacaagat ttttacgaag
    53281 ataaaatcga caaaaaaatt ttggttatta acatgccgcc tcgacatggg aagactttta
    53341 cagcgcggct attcgtttta tggatgtttg gacaaaatcc aaagacgaaa attattaccg
    53401 gatcttataa ccaaatcctg tcatctttat ttgcgcaaca aacacgtgat ggaatcatgg
    53461 ttgaaaacga aaatgttaag gctgaatact ttaaagatat ttttccagag gtgcaaatca
    53521 aatatggaga tgctgcaaag ggtttctggt ctttagatgg ttcagaagaa aagaattatt
    53581 tagcgacttc tcctggtgga acatccactg gtattggcgc gaattatgta attgttgatg
    53641 acatcattaa aaccgcagaa gaagcctata acgagcgtgt tttagattct cactgggaat
    53701 ggtataacaa cacgttggcc caacgaatgg aacggccacg taaacagata ttgattatga
    53761 cacgttggtc aagtaatgat ttagccggaa aaatgttgac gagacgaaaa aataatgttc
    53821 atcagatctg ttataaagca gtacaagaag acggctcgat gttgtgcaac gagattatga
    53881 cccacgatga gtacttagat gtggtacaag aaatgaatgt agatattgca gaagccaact
    53941 atcagcaaga accaattgac caaaaaggca ggctatatca gaagtttctt acttatgata
    54001 cgttgcctga taacattatc aaaatatgga actacaccga caccgccgac aagggtgccg
    54061 attactttgc gtctcctgtt tttgctgaga cgtctgatca tgatgcttat attatcgatg
    54121 tcatgtatac aaaagagccg atggaaacaa ctgaaatagc ccacgctaat atgattattc
    54181 gcaatcgagt taataaggta cggattgaag gaaacaatgg cgggcgtgga ttcagaagaa
    54241 attcagaaag aattacaaaa gagcgaggct atcgaggtgc agtatttgaa gactttcatc
    54301 aatcggataa taaactgtct agaatactgg cgaatagtgc ttgggtggaa aatcatgttt
    54361 actatccaga tgattgggcg acacgttggc cggactttta tgaggctatg gttagttatc
    54421 aacgtgaagg taagaacgct catgatgatg ctccggatgc agttacagga attgcagaaa
    54481 cgattataga agcagatgac ttcgagttta ttgcttatta ggaggtgggt ctttgtttta
    54541 ttctggaatc acattgaaaa aatacaaacg tttaagaacg aaatattcaa cgcaaatcga
    54601 agaaggatta ttcgatccta acgcttttat ggaagatatg aaaccctttt tcgatgatcg
    54661 agaaagaaag tatcttgctt atactagcga gaaaaacgag atcgacagtc gtgagaaacc
    54721 aaaaacaaaa atcattaagg tcaataacaa gattcatgcg ggaatgtatt cagtggtcgt
    54781 tgatcaagca gttaaccatt tcacaggaat accaattaaa tgggattatg atgtttcgga
    54841 acagaaaagg acattcattc aacgaatgag agacaaatta ctgggtaatg atatcagcgc
    54901 cccttctacc ccagaagaat ttgaaacact cacgagacaa ttaagtacta tgcggtttgg
    54961 aatgttagac cccgatactg caacatttca aggagcgaca gggcttgctt tcagattact
    55021 ggagcctgtt gagacaaatg atggttggga actaagagca agcaacattg agccgtggaa
    55081 agctgaaaag tacgaaaacg gcgcagtgta cattaaagag aaatacgatc catatcaaaa
    55141 gaagttttat gaagagatga aggttgtcac gccgaaatca atatttactt atgctcgata
    55201 tactgattac cctggcacgg tacaaggagc atttaaaacg ctgggtaatg ttcctaatcc
    55261 tttaggcgtt atcaatctta cggagtttaa aaacaataca aaccgttact gtgactttga
    55321 agttgcagag gagattagtg atgctattga tcgtgtttta tctgatcagc agaacgagat
    55381 tgaacaattc aagttagctt atatgctgat cactggggca gatttgtccg aggataaagc
    55441 caagaagatg atggagcaac tagggatcat caatctgaaa gaccctaatg cgaaagccgg
    55501 ctacgtgaca aaggatctac aaaaggattt caacgagtat cattttgatc ttcttaaaaa
    55561 gcaattctac acggtgtgta aagcaattga tttcaatgac gaagtcttta agtcgaatag
    55621 ttctggggaa gcccgaaagt ggcaaatcat tagtttagaa gctaagacaa ataccaaaga
    55681 acagtacttt cgagaaggat tgaaagagtg tgccgaggtt atggctgctt atttggattt
    55741 tgccgagaaa gttacaattg atccggaaaa gatcattttc accttctctc gttcgcttcc
    55801 aacagattta agctacctag ctgaggcatt gcctaagttg gcaccatacg tttctaagag
    55861 gacgattcaa agtcagatcc cgttcgtcac agatgttgat tatgaaaacg aaatgattga
    55921 cgtcgaaagc agacaatctt acccagacgg cgagtatgga ggtggcatcg ttgagtcaaa
    55981 tagaaacgaa gtactggacc aaacgtcgag aactggaaga tttagcaaga ctcaaaatgg
    56041 aaaatcaaac gctaaagaaa ctaagtagtg tctttcctga tgcgttgaaa gaaattcaag
    56101 cggagttatt atcccaagct gatttacacg atattaccta tgctgagatg atggaatttt
    56161 atagtacaag tgatcagaaa aaatatcgtg aatacgttga aaagaactac aaatcactga
    56221 agatgtatga tgcaaaatac aaagagttca tcgaagaata ctttccttct ttcgactacg
    56281 ccaaagtgaa tcggctgctt caaatgaggt cagatatttt tcgaattctt gcagagagtg
    56341 caatagatgc agatgtgaat aaatattttt cggatcggtt agaagatatc ttgcaacgtt
    56401 cgtatgtatc aaacgcaaac gtatttgccc agattttgaa cgtagatttg ccgaattact
    56461 tatcaaagga agaacttgaa cgttacctga actatccttg gtcaggtaag acattctcgc
    56521 gtcgtttatg gggcaatatt tcatctctag aacaaaagct atctaacgcc attacaacta
    56581 gtgtagcaag tggcgaagga gttctcgtgg cattacgaac aatgcgagaa gacccagaaa
    56641 tatgcgatat gttcaaaagg gaggaatcga agtacaacaa ggcgatcgaa aatcttgtga
    56701 gaacagaata cgctaaattc gctcaagatg gtattgagaa atcttatatt gaaactggcg
    56761 tagacgaata caatgtctta actgcaaaag atgaaaaggt atgttcaata tgcggtggca
    56821 aagcaaagaa caacccatac aagttgagtg aagcgatcat tggtgagaac cgtgcgcctt
    56881 ttcatggccg atgcagatgt acagatgttc ctaatatgcc gaaattagga aaagatattg
    56941 acgaagagta tgagcgttta tttggcgatt tattagatga gtttgcacat gattcttttg
    57001 gaattaattt gaaacggagg aagtagaatg aaagattttt ttgaagcagt actaacaatt
    57061 aatgtaaatg ctgatattgc agaagcctac aaaacagcta ttgagtctga gaaccatcct
    57121 aatggcttga gagaccattg gaatggcaat tatgcctacg tggttattgg cgatcaaact
    57181 gttaattatc aagataatac tccagttgat aagaataccg ttaatttaac gattcaatta
    57241 ttatctcatt cattaccaaa tttaaaagaa acagttgatt ggtatgaaaa tatgggatgt
    57301 attgttgtta gaactgacta caaagaagga aagtctagta attaggcttt tttattttgt
    57361 ccgaaatgac gttaaactag cgcaatactg ggcttggttg aatggtgggg cgcaactatt
    57421 aaaactcaaa gcaatgcggg gcgtgaaaac gaatcgtggg gcgaaaggag aatgattatg
    57481 aaacacaaat cattaatgcc aatgaatttg caatactttg ccgaaggtga cgatcaaaag
    57541 ttttcttttg atgacttcaa atcttttgtg gaatccaatg aagaagctca aaaatttatt
    57601 cagtcacaat ctcaaagtgt tgccgacaaa caattggaag cttggaaaca aaataactta
    57661 gataaaatta aacaggatac catcaaggaa tatgaggaat ctaagaaaaa caagtcacct
    57721 gagcaaattc aactggaaaa actacaagct gaatttgaag cagaaaaagc gttgcgtgtg
    57781 acaagtgata ataaagcatt tgttgcagaa caaattgctg gattagaact agatggagag
    57841 ttaaaagagt ctatttctca atttatgcta aataatcttg ttagttcgga tacagatttc
    57901 actaagaatg ctgttgaagg ttttacaagt gttttgaatg caattaatga gaaacatgca
    57961 gatgcattaa aagaactaca aatgaagtct gcatttggtg gaactcaaca atcgaataac
    58021 caggttcagc agaacaatga aacattaaca aatccagaag aacaattagg acaaattctt
    58081 caacaattta actaggagag tgaaaaatta tgaaaaaaac atctttaaat aatttagagt
    58141 atttggatat ttcaccagcg attaatgcta tgcaagtacc aaatacacct tttttaagct
    58201 atttacttgg tgctggaaaa acagagccag caaactcgac agaaattaaa tggcgtgaat
    58261 atgatatcaa caacgatgat tcttctgaaa aacttgaggg cggagaatat ccagatgctg
    58321 aatcaggtcg aacttggttt aacaactata ctgaaatttt tagaaaatca acctctgtat
    58381 ctggtacatt agatgctatt aatgtgaatg gagtcggaaa tgaattaact aatcaagtag
    58441 cactacgtgg tatggaaatg aaaattgatt tgaaccgaaa attgattact ggtgtaaaag
    58501 ctgatgaaaa tggttctaaa ggtcgtcgaa tgaatgggat tttgaacttg atcaattcag
    58561 caaataaggc agaaacagcc actgcgggtg cagtaacaag aaaagatatc gatactttat
    58621 ttaaattgat gtatgaaaaa ggttatatgg gagaaaaact atgcttgatt tctccagata
    58681 tgcaggagtt aatgactgat gagttagatg gaaaatcaac aaaaattgtt cagttcgggg
    58741 aaagagtaac ttttggattg caaattggaa atatcgtgtc taattacggt acaggtattg
    58801 ctctactaga accatcattg ccaaaaggaa caattgccgc aatcgatact aattatgtga
    58861 aactacgtcc attacgtgaa tggagagcag aagaacttgc aaaaacaacg gattctagac
    58921 gtatcggtct tgtaggtgaa tactctcttg aatacaatgc ttcaaactct ggggcaattt
    58981 taaatttaaa gtctgaataa aagggagtta gtactccctt ttttgatagg aggaattaca
    59041 gtggtaaaaa aagatgaaac taaaaaagat gaagtcgtga aatatagagt aggtaaaact
    59101 aaaaattttg ttgggtttgt tcatcctaaa actcgtagat ttatcacagc agattcaaat
    59161 aacgaattta tcatttctat agatgataaa gaagcaattg caattttgga agatgcaatt
    59221 gatgttaatg aaatttagga agtgatctga tggatgaatc gctaaaaacg gaaatcattg
    59281 agtctacaaa agaagatttt ccagatttga gtgaagaacg cataactaat ttattagaaa
    59341 taattttgct agaaattgaa tcatacaaca cttgtaaaaa tgatatttca tgggaaaagt
    59401 taaaaagcgt gattagtgaa gtgttgtata aaataataaa aaatgaatca gaaaaaacag
    59461 tatcttcagt tagacgtggc gatacaacga ttagttatgc ttcaacaaca aatgatgtta
    59521 gtgagttact tctaggctat ggtgatttga tacgaagagt tattggttgt ggaggattgg
    59581 agttttttta atgaatgaag cagatatttt agaaactact tatgaagata gttgtattat
    59641 tgaaagactg acggacattg aagattctaa tacaaatatt actattcaag attacaaaaa
    59701 agtatatgat aatcctattt cttgcgctct ttcacaaggt caaatcgatg gactagcagt
    59761 catagaagat ggagagatgg taaatgtttc aactgacaca tataaattat ttgttcatcc
    59821 taagattaaa ctaaaaaaag gagatcgaat aacaataact caaaaggcca gtggcttaat
    59881 tttttctcta tttgctacta agccttttta ctatcctagt cattgtgaag tgaatttgat
    59941 aggaagtgag aaaaatgggt aatctcaaat atgaatcaaa tgcagaaaag atcattgaga
    60001 attttaaaaa tatgactgta attgctcaaa aagaaggaat atccttcgtt aacgattcaa
    60061 tgaataaagt cgttagttta gctaaaccat taactcctgt aaaatcaggt aatttaaggc
    60121 gaggatatcg tgtagtaaaa gctagaaaac tatcaagcgg tcgaattgtt ggagcagtta
    60181 taaacaatga acattatttt aaatatgtag aagaaggtca taggactaaa aatggtggct
    60241 ttgtcaaagg gaaattcatg ttaactcgtg caacgaatct tgcaaatatg tcttatattc
    60301 ctcgaagatt taaacaaatg gcaataaaaa tcgttaagaa aggaaagtaa catgaaagat
    60361 gaaatcattg ctgcaatcag tagcaaatta aaagaaatct atcctgatgg gacaatatat
    60421 cttgattcag ttatgcagtc aactaaagat ttttatttcg ttttatcagt aatggaatct
    60481 ggaactgaaa atgtaggaat tgatattcaa aatgtttctt tcttaattga tattgcattg
    60541 attgataata aacctaatag aaatttaatt aatgaattag tctcacgttg tgggactttt
    60601 tttaatacga ttacaatcga tgagcaaata ttatttccaa aagcctattt acctgatgaa
    60661 gcagatggtg ttcaacatat tcgttttaca ttagaatttc cacaatatat tgaatggagt
    60721 gaaagataaa tgggagaaaa aagaagtaaa accggaatta tttctgtcga aaaacctact
    60781 tggtttcctt tggaagatga gacagggaat ttcccggtat atggtactgc gttgccaatg
    60841 ggaacagcag taagtatcaa accaacagct aattatgaaa caacacaaga ttatggggat
    60901 tcagtggttc aagatcaatt tacagctttt ggtggtgcag aagttgaact ggaagcaaat
    60961 ggatatactc ataaagtttt atctacaatt actggaggga aaattgttaa aggtggtgcc
    61021 ttgcgttctg gagaagatat tgcgcaagat ggtgcgtttg catatagacg taaaaaatcg
    61081 aatgggaaat accgttatac ggtcttttat aaaggacagt ttgctctaga ttcagatgaa
    61141 acgtcaacga ttgaaggaag taaagtaagt tttactcatc caacttggaa gggttcattt
    61201 gtagatgtac caggacttgg atatatgtat tcagttgatg aagatgatga aggcgtggat
    61261 aaagcaatga tcggaaattg gtttacaaaa gtagcaattc caattgaaga agcagaactt
    61321 tcaggaggta cagaataatg tcaaaatatc aaacaacgat taaattaacg aaaaaagatg
    61381 aagaaggtaa atatgagcaa gtacaattta aatctgctga atttttacca ggaacagttg
    61441 tagaagatgc tgcaggagtt atggaagaaa tgcaaacagc gactgataaa caatcagtta
    61501 aaaaagcttt aagtcgtgct tattcattta ttgcagatac tttatttgag ggacaattta
    61561 ctggtgaaga ttattgcaaa ggaattgatg ctcgtgagat tgcctcattg acaggaaaat
    61621 tattgaagtc tgttactgca ggttttgatg aaacttatac agagacgaaa aaaaagtaag
    61681 tgaggcgctc aagtcacctt catttaagta ttcgattact taccgagaat tagatataaa
    61741 gacgcaatta ctcgaagcag gttggacgtt accagaaatt gaacgtactg acttggatga
    61801 gttaatgcgt ctttttgctt ttagagatgc ggtaaaagag catgaagatg ttgagtacta
    61861 tgataatttc actcaatttt aggaggtgat actttgaata acgatgacct aattctgaag
    61921 atgatactag atgaatctgg ttttactgcc ggtatgaata atgctgtcaa aaagttgaac
    61981 tcttttgatg aaacaatcga aagaaaaagt agaaatagtg gcagttcatt aggtagtatt
    62041 tggaaaatat ttgctggaag ttttctagct agcggagtaa ctagaatcgt aggagctggc
    62101 tttgatctaa tcaagggttc cataagtgga gcggttgatc gagtagatac gatgaataac
    62161 gctctacgaa atttccaaaa tatgggattt agtaattcag aaattatgaa gaatatcgga
    62221 aagaatgggc ttttatctca aggtattcaa ggacttccta ctgctttgaa tgatgcgata
    62281 agtcatgttc aacttcttgc ttcttctaca ggtgatttaa ctcgttcaac tcaaatattt
    62341 aaagctttaa atgatggaat tcttggtttc ggtggttcaa ctgatcaagt taatgaagct
    62401 gttattcaat tatctcaaag tttctcaaac ggaaaagtag acgcacaaac ttggaactca
    62461 atgatcaacg ctcaacttgg acctactctt tctgctattg ctaaaaagat gggaattaca
    62521 atgggagatc tgaaagaagg tttatctcaa ggtaagattt ctgttgaaga attccaaaat
    62581 caattaatag aaatggatac caaaggtggc ggaggactta aatcattaag tcaaatcgct
    62641 aaagattcta ctaagggaat taaaacctct atacaaaatg caaaaacagc tgttacacgt
    62701 ggtgttggtg aggtaataga aggattaaat aaagcattag tagattcaga tttaggtgga
    62761 tttaagggaa ttattgataa agtgggcagt tcaatggaat cgttcttaaa agtaattgct
    62821 gcaaatattc ctacagcagt atcattttta agtaatttat ttaacgaaat tcaaaaattt
    62881 ggttctgctt tgaaattcat gatgcccttt cttgttcctg cagctaccgc ttttggagcg
    62941 ttcatgtttc aacttaaagg tataccagca ataataaaaa gcttcaataa ttttaagaat
    63001 gccataattg gcgttggaaa ttcattaaag attatggggg caatagctgc tgctaattca
    63061 tttgttttaa ttattggtgc agtcgttggt gcaatagctg ttttcggtta ctttatggca
    63121 accaatgaag agtttagaaa caaggttata tctatttgga atgatgtgaa agattccgta
    63181 attggtgtat taaaaaatat aaaggactgg ggaattgata cttggaattc tgctaaagaa
    63241 atggcatcaa atgctgttga gagtgtcaag gatgcttggt ctggaataaa ggaatggttt
    63301 tcaaatactt ggcaaggcat taaaaatgga gcaacaggtt tatttgataa aacagtagaa
    63361 acatctatga atgcggttga tagtgtaaaa aacgcatggt caaatacaaa gcaatggttt
    63421 tctgatattt ggcagagcat aaaagattca gcaactgaaa aatggaatga aattaaaggt
    63481 tctatcatgg aagttgctgg tccattgatt acaggtttaa agaacgcatt cttgcatgtc
    63541 actttttatt tagagacctt atggaataat ctagttgaga ttggtaaaaa tgtttttgag
    63601 attttaaaaa atgttatact tacgccagtt ttgtttatta cctccatgat ttctggtgga
    63661 tgggaagaaa caaaaaacaa catgattggt gtatggaata atattaaaga aagtgctcta
    63721 aatatttggg agtctataaa aaatattttt gttagttaca ttacaaatat ttattttgct
    63781 gcgcttaata tttggacagg gttcaaactt actttgataa acatttggaa tgaagtagta
    63841 agtcaagcta aatcaatttg gattaatgta aaatactttt ttattaatct ttggattgac
    63901 attaagtatt ttgctattca aaaatggatt gaattaaaat tcgggattat tcaaacttgg
    63961 attgatttaa aatataatgc tattactact tggaacaata ttaaacagtt cttcaaagat
    64021 acttggcaga acattaaaga tatagcatac aatacatgga tttctataaa gaattccatg
    64081 atcaatactt ggaataacat caaggaatct ttctggaata ttgttactgg aattgttaac
    64141 tccgctgaaa atgcatggac taatctaaaa aatggtgttt caaaagcaat taatcgggtg
    64201 aaagaaatct ttgattcatt aagggaaatc aatttattcg aaatcggtaa gaatatcatt
    64261 gatggactta tcaatggtgt agtagaaaaa tggaatgcat tgaaaaagac tattaaagga
    64321 atagctggaa gtatcaaaga ttctattaag ggtgcattag gtattcattc tccatctaga
    64381 tggatgaggg atatggtagg taaaaatatt gttcaaggta ttatcgttgg tattgataaa
    64441 gaaaaaagta aattggatca gacaatgact gatttagtga aaactccgtc ggttcaacca
    64501 gttattacag ggtctaatag tcaaccagtg gtacaagcta aacaaaatac atcttcaaat
    64561 gcagtgaatg aaattcactt gcatttaaat gtttatggag atctacctga ttcgatgatt
    64621 aaacaaatcg ccaagaagat gaaaacagaa ttgacgagac aaatgaaacg agatgctgat
    64681 gcagtaggag ggacattata tgcaacttaa aagaggacaa ttttttatca atcaacatta
    64741 ttcttctgag tttaacgtat atattcaaaa taggcctgcc tctgtttcag ctagtcgtgt
    64801 aattgaattg agagaacgtg aaggcaatga ctcaattatt atcgataaag cctattacaa
    64861 aaacgttact agaaaaattg aatgttatta caaagcacca tcgattgatt tagtgcagga
    64921 atgggaagat cgaattactg aatggttaga tatgaactct tacagtgatt ttattcttta
    64981 ttacgatcaa caatatatct atcaagctat agtaaccgaa gcgccagaat ttaaaggaac
    65041 aagaaaaaca gggaacatag ttccatttga atttacagtt agtcttcgac cattcaaaga
    65101 aaattatagt ggtcgttttg ctattcaaca gataaaagct ttcgagttat ttaatccaga
    65161 gaagtatgct tcaaaaccgc ttattaaatt gagtggttct ggagatgctt ctttttatat
    65221 taataatgat aaatatgatt tgaaactatt agatagagaa ttatatatag attctaaact
    65281 agaagagtct tatcgaaagc tagatgacaa tttagaacat caagatcatg tcactttatt
    65341 tttagatttt ccatttctat accctggaaa aaatgaaatc aaatggacga ataacataca
    65401 ttcgtttgag ataataccaa ggtggtggag aaaagtatga taccaagaat atatagtcct
    65461 accgagacag atttttctac gaatggttta gggattttga aggacaccac aaaatgtgag
    65521 atatatgaag tagcaaatgg aaaatatgaa ttagagttgg agtatccatt aggtactcga
    65581 tttgatgaat attttgaaaa tgactatcaa ataaaagcaa agtcaaatga tcaagaagag
    65641 tatcatatct tttttattga tgataaagat attgatacct ttttagatac cgtaactatt
    65701 tatgcccaga gtcgtacaaa tcggcttgga agacgagcgg tcacttttgc tggagtcgac
    65761 tctaaaactg gtcgagaagc aatggcaatt attgaaaaca atatggataa aaaatcagat
    65821 atacgcttat attctgatat tacaactgta tcaagtacaa cttttgaagc aagaaatgtt
    65881 ttaaattgta ttgctggtga acaaggatct ttacttcagt attggggcgg agaaattaaa
    65941 cgtgagcctt ttaagctatc tttattaaag cgaagaggtc gtgataatat tggcacaatc
    66001 cgatatggaa aagatttatc tgggttaaaa gttaagttag attggacagg cgtgaaaaca
    66061 agaattattc cgtatgctga tcctcaaagt gatgcaggta caactagccg aatttatggc
    66121 tcgccagtag atagcgaata tattaataat tatcctgatg tatatacaga acacgttcaa
    66181 ttcacagaag aacagggagt taaagacgtt aatagcttaa ataaaatagc taagaattat
    66241 ttcaaaacaa ttaatccagg ctgtgataaa ccgaagattt ctattaccgt tgagttcgat
    66301 aaattaacgg acaccgaaga aggaaaagaa ttcgctaaga taagaaatta tgggttattt
    66361 gatacgttta aaatatatca tcgaaaatat aaactatatt ttgaatcgaa agttagcggt
    66421 gttcaatatg attcattatc tgaaaaggtt ttgaaattgg aagctgggga tgctcaagtt
    66481 gctttttatc aacaacaagc tgttactatt caagataaat taaaagatta tgctacgaat
    66541 aattatatga gtagttttaa tgattatgtg tcctctatga ttgcaggtca aggaaatgca
    66601 ggcggttatg tagttttatg gccaaaagaa aaaccttcaa atatttttat aatggatagc
    66661 cctgatttaa acaaggcaaa agaagtatta agaatgaaca aaaatggaat tgccttttct
    66721 aaaaatggtt ggaatggtcc ttttaattcc gcttggacat tggatagtat attcaatgct
    66781 aattttattc aaacaggatt aataaaggca gatatctttc aaaactcttt taataaaact
    66841 ggagatgtat tgaaattagt taatggacta cttcaaattt ggaataacaa aaagaaaata
    66901 atggagttaa ccaaaaaagg aatggagttt tggaattcta atagttcaat tggaacgatt
    66961 ggaacaactg attctgctgg taatcctttt cctggagctt ctactcccac acctattgaa
    67021 gataattctt tagttattcg tacaaatgga gacggcaaat atattttgat ttctcctaaa
    67081 gttggtaaag gattagtttt attaggaaat ggtaaagcaa tttattttgg agacttagat
    67141 gtacaaggca aactcacagt taacggaaaa gaaatcacag ggaacaacag tggaggaagt
    67201 gatcctggaa ctatccctcc tcaattgacg acggaagctg aaaaaagagc atggaaaatt
    67261 tggacaatgc tgaaagctcg tggctattct gaatatgcag ctgcaggtat tctaggaaat
    67321 attcaggggg aggttggagc aagtatgaac cctgatacag aacaacttgg tggtccagct
    67381 tatgggattg ttcaatggga tggttctgct tatccgttag tcggatcacc aacttggaac
    67441 ggacgagagt atgttcagcg tttgatgaac accgcaggga ttcaagaaga ttatcgaagt
    67501 attgaagccc aagtaaaatt attagattgg tgtatgttca acggtcaatg gctcggaaaa
    67561 gtaaatccaa ccacagtatc aggatttaaa tcgatcaatg atgccaaaag tgcagcgtat
    67621 gcttttgaaa tgaacttcga acgcccagct tctgcacatc cagaacgcca aaattatgcc
    67681 caatcttggt ataacaaatt acatggatta actagtccag aacctggagg gaatttcatt
    67741 tgtccaattc aaaaaccagt gacagttact tcagaatgtg gatggagaac tagtccaata
    67801 aatggcggcc aagaatttca taatggaatt gatcttgtaa atggaaatcc taatacacct
    67861 gtttttgcag cattagatgg ggaagttgtt caggctggtg ctaattatta tgactggtat
    67921 ggtaattacg tggttattaa acataataat gggaagtgga caggttatgc tcatttgtct
    67981 cgtattgatg tttctgttgg acaaaaagtc cagaaaggtg ctcaaatagg cttgatggga
    68041 acaactggtc catctacagg agaacatcta cattttcaaa ttatgaaaaa ttattggcca
    68101 cagccagttg ttgattttga gaatccaaga aattatatcc aattttaagg tggtgattct
    68161 atgagtaaat ggaatgtcgt tttaagtaca acagaaccat ataattatgt ggggatgatt
    68221 caagttcgac aaggcaataa gaatacagag gttatggaag cgactatagt tgaaaatggt
    68281 cttccctacg atttatcaga atgtaaggta tattttgaat cagttgtagg tgggaaatat
    68341 ccagtccaat tagaaacaaa aattgtggat gctaaaaaag ggaaaattaa ctatattttt
    68401 gataaatatt ccatgcagtg tttgcatcga caaacagcca atttcattat atttaaagga
    68461 gaagacttga ttggaacaac tcaagacttc tcctattttg tcatcaatgc tgtttcaaaa
    68521 acagaaggag aaatgggttc ttattggcaa tcaattgaag atttaattgc tgatatgaca
    68581 gattttatta atgaaaataa aggcgatttt acggactgga tgaatgaaag gaaagaagaa
    68641 tttgatcgct ggagagaaga acaggagaat agtttccaag attggagaga aggacaagaa
    68701 tcagattatt tgtcatggtt tgaatcaatc aaggatattt tgaagtctat cgatccaggt
    68761 ggagtaatgt tagccgaatt aatggatgca cgtgttgata ttcaaggagt tcgtcatgcg
    68821 tctatttcag agcgcttgct ggcagatatg gattatctat atcagaaatt acgagcaaca
    68881 cttttcacga ttgaatacgg tgagattgaa gtgactgata ttttgcagga tgatctcttt
    68941 tcagataatc acgaagtcga aaaagttgga actgtagaat ttccaattga agaaggagct
    69001 ttgattattg caactgttga tgatccaaaa cagaatgttt tcactcttga gaaagttggg
    69061 gtgatttgat ggttaaagta aagagaatga tggagactga tgaatctggt gttaaacgtc
    69121 aatttatgcc aattacacat gtttctgcag ttttaggctt agaaaaaatg atatcaggac
    69181 aatcaaaagt tctttcggtt aatggtaaat atggtgcagt cattttaacg aaagctgatt
    69241 taggattaga gaatgcaatt actgaactac cttatgcaag cgagacaagc gatggtattc
    69301 ttactgctga aatgtttcaa aagatcgtaa atggagaagg tggaacatat attttgccga
    69361 ttgctactcc tgaacaatta ggaggtataa aagttgggga actcttagaa gttactgaag
    69421 aaggtgtttt atctgcaact aagcaaacag acttcaattt ctctgaagaa ttaaagggaa
    69481 aattagaatc tctaaaaata ttaaaagcag gtgcaaatat atcaattgca gaagatggaa
    69541 caattagttc tacaggtggt agtgggactg gtggtgttaa tcagtcatat gtagatcaaa
    69601 aattccaaga agctgtaaat caagcagaaa attacacaaa cgaaagaatt ccaaatttta
    69661 cttttgaaaa aatcggggag gtatagaaaa tgacagatat cgtaaaaata aaacaaagcg
    69721 gtgtacaggt ttatccacaa actcattgga atgctataga aggcaaacca acaacggtga
    69781 agggagataa aggcgatcct ggccaagcag caacaattac cataggaacc gtttctagtg
    69841 gttcaaccgc ttctgttacg aatgtaggaa catcatctgc agctagattt aattttgttt
    69901 taccaaaggg agataaaggt gatcctggaa taaatgccac aactacagct gtagctacaa
    69961 ctacagcaaa tgggttgatg tcgtccacag ataaaactaa gttggatgga attgcagctg
    70021 gagcacagaa aaatccaggt aatgctacaa ctacgacagc aggtttaatg tcagcaaccg
    70081 ataaagtgaa acttgatgga ttagcgaata ttacatttga gaaagtgggg acggtttaat
    70141 ggctgatatt gtgcaattaa aagaagacgg agttgctaaa tacttaaaaa cgcatgtaga
    70201 agctattgat ggtaaagaag cattggtgca gacagatgga gaccaagcca ttgcaggaca
    70261 taagaatttt tcaggttctg taacaatcaa taataaacgt gttttgacga ctgatgataa
    70321 cagatatgaa gtggtaaatc tggttgtcac taacggtaat acagggacag caaagcttta
    70381 tcgtgaagga aaaacagttt ctatatattt tgtggcttta aacggaaaaa gtagtggcgg
    70441 gaatgattcg gttattttaa ctgttccaga aggctatcgg acaccaatta gttttgaaca
    70501 actggttggg tcaatagacc gttctacttt gaacagtgct caactatcta ttggcgcaga
    70561 cggagccatt aaatggagaa gaaattcaag ttatggatca gcttattcat ttgttatcac
    70621 ttattcaatt taagggagga aatctaatga aagtagttta caaatcaatc aagccttacg
    70681 gattcgagca aatcattttg aacaatcaag aaaatatccc tgaaaactgt acagagatta
    70741 aaccaccagt tcctaactgg agaccaagat ttgattttga taaaaaacag tgggttgagt
    70801 tagctactga agaagagaaa aaagggaatg ctgttgatag cgtagatgaa ttggctaata
    70861 ttaaggcatt atacgaaacg ctgaaggcag aaaatgatga attgaaacag ctaaattcta
    70921 aagcaatgct aaacaatgta gcaatcaagc aagaaaatgt cttattgaaa gaaaagtcag
    70981 aaagtttagc gcagttgaat tcaaaaacga tgcttgcttc tgtacaaaat accaaggaaa
    71041 tcgaagaaat caaaaaacaa ttacaaggtg ggaagtaaca tgtattcata tgaagatatc
    71101 aaactaatgt atgactgggg ctgtttcact aacgaacaag taatggtttt cgttccatta
    71161 tgcattacag acgaagaagc agataaaatc attaataaag ataagagcgc atcttaattg
    71221 atgcgttttt atttaaggta aaggagttgt cacatgatta atttagggga atggggaatg
    71281 atagcaggat caataaccgc tatcgtttct ttgattttat tagtaattag accgattgct
    71341 gcatctttct cgaaaattac tgagactctt tcaaaagtaa gccacaattt agatttgctg
    71401 actaaagatt tagaatcgag caaatcagat cgattgatga ttcatgaaga actaaagaaa
    71461 cacgatgaaa gattagatac acatacagaa aaattggtgg aacacacgca acaaatcaaa
    71521 acttt
//
LOCUS       DACEPD010000003        63403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.003, whole genome shotgun sequence.
ACCESSION   DACEPD010000003 DACEPD010000000
VERSION     DACEPD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.003"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            83..970
                     /locus_tag="IW797_000161"
     CDS             83..970
                     /locus_tag="IW797_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1222967.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IW797_000162"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IW797_000162"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ1222968.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IW797_000163"
     CDS             2272..2718
                     /locus_tag="IW797_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1222969.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IW797_000164"
     CDS             2991..4343
                     /locus_tag="IW797_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ1222970.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IW797_000165"
     CDS             4514..4801
                     /locus_tag="IW797_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222971.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5027..5470
                     /locus_tag="IW797_000166"
     CDS             5027..5470
                     /locus_tag="IW797_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1222972.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5476..6660)
                     /locus_tag="IW797_000167"
     CDS             complement(5476..6660)
                     /locus_tag="IW797_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ1222973.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6928..7626
                     /locus_tag="IW797_000168"
     CDS             6928..7626
                     /locus_tag="IW797_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ1222974.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7633..8310
                     /gene="nth"
                     /locus_tag="IW797_000169"
     CDS             7633..8310
                     /gene="nth"
                     /locus_tag="IW797_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ1222975.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8436..8969)
                     /locus_tag="IW797_000170"
     CDS             complement(8436..8969)
                     /locus_tag="IW797_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222976.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9266..11647)
                     /locus_tag="IW797_000171"
     CDS             complement(9266..11647)
                     /locus_tag="IW797_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1222977.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IW797_000172"
     CDS             complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IW797_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ1222978.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12348..12887
                     /locus_tag="IW797_000173"
     CDS             12348..12887
                     /locus_tag="IW797_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ1222979.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12977..13396
                     /gene="gpsB"
                     /locus_tag="IW797_000174"
     CDS             12977..13396
                     /gene="gpsB"
                     /locus_tag="IW797_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ1222980.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13418..13785
                     /gene="rnpB"
                     /locus_tag="IW797_000175"
     ncRNA           13418..13785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IW797_000175"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13994..15160
                     /locus_tag="IW797_000176"
     CDS             13994..15160
                     /locus_tag="IW797_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ1222981.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15206..16702
                     /locus_tag="IW797_000177"
     CDS             15206..16702
                     /locus_tag="IW797_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ1222982.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16783..17466
                     /locus_tag="IW797_000178"
     CDS             16783..17466
                     /locus_tag="IW797_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1222983.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18111..19301
                     /locus_tag="IW797_000179"
     CDS             18111..19301
                     /locus_tag="IW797_000179"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ1222984.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19403..20356
                     /locus_tag="IW797_000180"
     CDS             19403..20356
                     /locus_tag="IW797_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ1222985.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20425..21774
                     /locus_tag="IW797_000181"
     CDS             20425..21774
                     /locus_tag="IW797_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1222986.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21957..22280)
                     /locus_tag="IW797_000182"
     CDS             complement(21957..22280)
                     /locus_tag="IW797_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222987.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22409..23572
                     /locus_tag="IW797_000183"
     CDS             22409..23572
                     /locus_tag="IW797_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222988.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23642..24991
                     /locus_tag="IW797_000184"
     CDS             23642..24991
                     /locus_tag="IW797_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1222989.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25036..25467)
                     /locus_tag="IW797_000185"
     CDS             complement(25036..25467)
                     /locus_tag="IW797_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1222990.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25855..28497
                     /gene="alaS"
                     /locus_tag="IW797_000186"
     CDS             25855..28497
                     /gene="alaS"
                     /locus_tag="IW797_000186"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ1222991.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28697..29293
                     /locus_tag="IW797_000187"
     CDS             28697..29293
                     /locus_tag="IW797_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1222992.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29415..30563
                     /locus_tag="IW797_000188"
     CDS             29415..30563
                     /locus_tag="IW797_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1222993.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30601..31014)
                     /locus_tag="IW797_000189"
     CDS             complement(30601..31014)
                     /locus_tag="IW797_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ1222994.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31093..31524
                     /locus_tag="IW797_000190"
     CDS             31093..31524
                     /locus_tag="IW797_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ1222995.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31623..31856)
                     /locus_tag="IW797_000191"
     CDS             complement(31623..31856)
                     /locus_tag="IW797_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ1222996.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32039..33709
                     /locus_tag="IW797_000192"
     CDS             32039..33709
                     /locus_tag="IW797_000192"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ1222997.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33856..34242
                     /locus_tag="IW797_000193"
     CDS             33856..34242
                     /locus_tag="IW797_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1222998.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34229..34927
                     /locus_tag="IW797_000194"
     CDS             34229..34927
                     /locus_tag="IW797_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1222999.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34932..35396
                     /locus_tag="IW797_000195"
     CDS             34932..35396
                     /locus_tag="IW797_000195"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ1223000.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35393..36304
                     /locus_tag="IW797_000196"
     CDS             35393..36304
                     /locus_tag="IW797_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1223001.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36539..37078
                     /gene="pyrR"
                     /locus_tag="IW797_000197"
     CDS             36539..37078
                     /gene="pyrR"
                     /locus_tag="IW797_000197"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ1223002.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37092..38375
                     /locus_tag="IW797_000198"
     CDS             37092..38375
                     /locus_tag="IW797_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ1223003.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38388..39314
                     /locus_tag="IW797_000199"
     CDS             38388..39314
                     /locus_tag="IW797_000199"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ1223004.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39333..40613
                     /locus_tag="IW797_000200"
     CDS             39333..40613
                     /locus_tag="IW797_000200"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ1223005.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40618..41694
                     /gene="carA"
                     /locus_tag="IW797_000201"
     CDS             40618..41694
                     /gene="carA"
                     /locus_tag="IW797_000201"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ1223006.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41699..44884
                     /gene="carB"
                     /locus_tag="IW797_000202"
     CDS             41699..44884
                     /gene="carB"
                     /locus_tag="IW797_000202"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ1223007.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44938..45714
                     /locus_tag="IW797_000203"
     CDS             44938..45714
                     /locus_tag="IW797_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ1223008.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45711..46643
                     /locus_tag="IW797_000204"
     CDS             45711..46643
                     /locus_tag="IW797_000204"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ1223009.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46640..47356
                     /gene="pyrF"
                     /locus_tag="IW797_000205"
     CDS             46640..47356
                     /gene="pyrF"
                     /locus_tag="IW797_000205"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ1223010.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47363..47992
                     /locus_tag="IW797_000206"
     CDS             47363..47992
                     /locus_tag="IW797_000206"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ1223011.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47980..48294
                     /locus_tag="IW797_000207"
     CDS             47980..48294
                     /locus_tag="IW797_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223012.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48332..48973
                     /locus_tag="IW797_000208"
     CDS             48332..48973
                     /locus_tag="IW797_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1223013.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49004..49708
                     /locus_tag="IW797_000209"
     CDS             49004..49708
                     /locus_tag="IW797_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ1223014.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            49705..50607
                     /locus_tag="IW797_000210"
     CDS             49705..50607
                     /locus_tag="IW797_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1223015.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50765..52471)
                     /locus_tag="IW797_000211"
     CDS             complement(50765..52471)
                     /locus_tag="IW797_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ1223016.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52661..53284
                     /locus_tag="IW797_000212"
     CDS             52661..53284
                     /locus_tag="IW797_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223017.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            53734..54783
                     /locus_tag="IW797_000213"
     CDS             53734..54783
                     /locus_tag="IW797_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1223018.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            54780..55661
                     /locus_tag="IW797_000214"
     CDS             54780..55661
                     /locus_tag="IW797_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1223019.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            55664..56434
                     /locus_tag="IW797_000215"
     CDS             55664..56434
                     /locus_tag="IW797_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1223020.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            56713..57567
                     /gene="aroE"
                     /locus_tag="IW797_000216"
     CDS             56713..57567
                     /gene="aroE"
                     /locus_tag="IW797_000216"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ1223021.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            57745..58908
                     /locus_tag="IW797_000217"
     CDS             57745..58908
                     /locus_tag="IW797_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ1223022.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            59025..59435
                     /locus_tag="IW797_000218"
     CDS             59025..59435
                     /locus_tag="IW797_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1223023.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            59609..61837
                     /gene="topB"
                     /locus_tag="IW797_000219"
     CDS             59609..61837
                     /gene="topB"
                     /locus_tag="IW797_000219"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1223024.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            61975..62292
                     /locus_tag="IW797_000220"
     CDS             61975..62292
                     /locus_tag="IW797_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223025.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            62452..62688
                     /locus_tag="IW797_000221"
     CDS             62452..62688
                     /locus_tag="IW797_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1223026.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            62685..63083
                     /locus_tag="IW797_000222"
     CDS             62685..63083
                     /locus_tag="IW797_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ1223027.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT        20987 a        10950 c        13564 g        17902 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaat cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa
     4981 taggaaacaa aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca
     5041 actgctaata gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga
     5101 aatggaaaaa gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg
     5161 gaaaatccct gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc
     5221 catcagtaag ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga
     5281 agagaataaa aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag
     5341 acacgagcaa ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc
     5401 aaaagaagaa tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag
     5461 tagcaaatga agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa
     5521 tccgaatacc gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc
     5581 gatgacgcgc gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt
     5641 atctgagaca gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa
     5701 accataacag ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt
     5761 gatgaataga taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga
     5821 aaattttttg agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat
     5881 caaagagaag cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg
     5941 agaaaagata gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa
     6001 aggaataacg gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga
     6061 gaaacttaaa tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc
     6121 ataaacggca tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa
     6181 ctcagctaat agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc
     6241 catccatcca aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata
     6301 taaggtggag atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca
     6361 tatcgaattt cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat
     6421 aaggatcgga tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa
     6481 ggcagctaga gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga
     6541 gtgagaataa ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc
     6601 agtgaataag acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat
     6661 gtaaaaactc ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt
     6721 gtttccttgt caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag
     6781 tgaagatacg catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg
     6841 accgatcagt gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt
     6901 atactgtgat tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga
     6961 caaacagtca tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa
     7021 gaatttttat ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat
     7081 ttgcagatga ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat
     7141 cgtctcgtaa caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca
     7201 gatcgatata atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa
     7261 aaaaaatacg aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa
     7321 caagaatttg gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa
     7381 gaagaccatt atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag
     7441 gcttatagtt ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt
     7501 acaaaacaac aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa
     7561 gtagaacagt cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag
     7621 gagtagcgga atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga
     7681 atgtttcccg aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca
     7741 gtgatcttaa gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt
     7801 gcctcttttc cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa
     7861 ataaaaacga ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag
     7921 ctgattgaac gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct
     7981 ggtgtaggca gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc
     8041 gcagtagata cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct
     8101 acggtcatgg aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag
     8161 acacatcata cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc
     8221 gaggtatgtc ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa
     8281 aaggcgctaa aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta
     8341 tgaattatct tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt
     8401 aaatgtacat actcagtagc taggatggta acttattaca atttattgcg ataaaaataa
     8461 aagttgttat ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct
     8521 ataaaataag tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt
     8581 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
     8641 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
     8701 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
     8761 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
     8821 aataaacaaa atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
     8881 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
     8941 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
     9001 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
     9061 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
     9121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
     9181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
     9241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
     9301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
     9361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
     9421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
     9481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
     9541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     9601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     9661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     9721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     9781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     9841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     9901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     9961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
    10021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
    10081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
    10141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
    10201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
    10261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
    10321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
    10381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
    10441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
    10501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
    10561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
    10621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
    10681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
    10741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
    10801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
    10861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
    10921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
    10981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
    11041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
    11101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
    11161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
    11221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
    11281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
    11341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
    11401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
    11461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
    11521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
    11581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
    11641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
    11701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
    11761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
    11821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
    11881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
    11941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
    12001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
    12061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
    12121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
    12181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
    12241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
    12301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
    12361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
    12421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
    12481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
    12541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
    12601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
    12661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
    12721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
    12781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
    12841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
    12901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
    12961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
    13021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
    13081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
    13141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
    13201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
    13261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
    13321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
    13381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
    13441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
    13501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
    13561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
    13621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
    13681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
    13741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
    13801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
    13861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
    13921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
    13981 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
    14041 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
    14101 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
    14161 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
    14221 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
    14281 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
    14341 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
    14401 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
    14461 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
    14521 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
    14581 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
    14641 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
    14701 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
    14761 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
    14821 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
    14881 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
    14941 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
    15001 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
    15061 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
    15121 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
    15181 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
    15241 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
    15301 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
    15361 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
    15421 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
    15481 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
    15541 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
    15601 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
    15661 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
    15721 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
    15781 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
    15841 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
    15901 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
    15961 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
    16021 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
    16081 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
    16141 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
    16201 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
    16261 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
    16321 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
    16381 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
    16441 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
    16501 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
    16561 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
    16621 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
    16681 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
    16741 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
    16801 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
    16861 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
    16921 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
    16981 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
    17041 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
    17101 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
    17161 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
    17221 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
    17281 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
    17341 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
    17401 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
    17461 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
    17521 aaagttactt ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt
    17581 cacaaggaac aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt
    17641 cggaattggc aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc
    17701 acagtccttt tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt
    17761 aaaaattgct gataaacgaa gatggaaatt ttatgtattt ttattggtct atgcaataaa
    17821 taaattaaat attcagaata caattgattt taacgaaagg gcttctatca atggttgtgt
    17881 tgtatttatc tgcccaggta gagtaaaata gatcattatc taaaaggtaa ttgttcatac
    17941 tatataagtc atttgaaagt aaaaagatac tgtaaataaa aaatgtggtt aaataattct
    18001 cataatcagt gtaagcgtta tcaaaaatat agcatacttg ctttgtagat gaatgacacg
    18061 atatatgttt ggtatgttct gcaagacaga tgaatatgga ggtggagaca atgtcttcca
    18121 aagaactaac aatgtattta gagaaagaat tggatcaact gaaagaaaag gggttataca
    18181 atacgatcga catactagaa agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga
    18241 tgatcaatct agcatccaac aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag
    18301 catgtattga agcaaccgaa acttatggtg taggagcagg agcagtccga acgatcaatg
    18361 gttcgctgaa aatccatcag caattagaag aaaaaatcgc agaattcaaa gggacagaag
    18421 cagcaatcgc ctttcaatct ggattcaact gcaatatggg cgctatctct gcagtaatga
    18481 caaaagaaga tgctatctta tcggatgaat taaaccatgc atctattatt gacggttgcc
    18541 gcttatccgg agcaaaaatc atccgaatta aacatcaaga tatgaaagac cttgagaaaa
    18601 aagcaaagga agcgatagaa agtaagaaat ataaaaaaat catgtatatc acagatggtg
    18661 ttttctccat ggatggagac attgcacgat tgccggaaat tattccgatt gttgaaaaat
    18721 atggactgat cacctacgtc gatgatgcgc acggctcagg cgtaactgga aaaggtgcag
    18781 gaaccgtcaa gcattttgga ttaagcgaca aaatcgatat gcaaatgggc acgttatcga
    18841 aagctatagg tgttgttggg ggttatgtag ctggatcgaa aacactaatc gactggttga
    18901 aagctagaag tcggccattc ctattttcta cttcgctaac tccaggtgca gctgcagctg
    18961 cattggcttc gattactttg atgcaagaac atccggaact tgtcgaaaaa gtttgggaaa
    19021 atgctaatta ttttaaagaa gaattgaaaa aagtaggcta taacatcggt atgtctgaaa
    19081 cacctattac tccagtcatt ttgggagacg aaaaagtgac tcagactttc tctaagaagc
    19141 tgatcgaaca tgggatttat gctaaaccaa tcgtttatcc aactgtgcct cttgggacag
    19201 gcaggatcag aaatatgccg actgcggaac atacgaggga aatgcttgat gaagcagtag
    19261 cagtttatca aaaaatagga aaagaaatgg aaattattta actaaattta gttttttcgt
    19321 ctatttaaaa ttagagaaca tgttcacgca tatattgtta atcatctgaa gatagtagta
    19381 aaaaagatca gggaggatca tcatgaaaaa agtattggtg acaggatgtt taggacaaat
    19441 tggttcagag ttgatcacac ggctacgtca agaactaggc agtgaaaatg tcattgcgac
    19501 agatatacgc atgcctgaag tcattgagaa aaacgatatc tttgagatcc tagatgtaat
    19561 ggattacgaa aaaatgcgca gcatagcaga acaatatcaa gtggacacat tgattcattt
    19621 ggctgctctt ttgtcagctg ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt
    19681 gactgggctg gtcaatgcat tggaagtcgc tagagaattc cagctgaaat tttttacgcc
    19741 aagttcaatc ggcgcgtttg gtccaaatac tccaaaagat cataccccac aagatactct
    19801 tcagcgtcct acaaccatgt atggggtaac gaaggtagct ggagaactac tatgtgatta
    19861 ttattatgtg aaatatggcg tagatactcg tggtgtccgt ttccctggat tgatttctta
    19921 tgcaaccttg ccaggaggcg gtacgacaga ctacgcagtc gatatttatt attcagcaat
    19981 caaagaagga aagtacacta gtttcattga tcaaggtaca tcaatggata tgatgtatat
    20041 gccagatgcg atcgatgcca ttataaaact catgaatgca gatcctgatc gattggttca
    20101 tcgaaatgcc ttcaatatat cggcgatgtc ttttgacccg gaagagatca aagcatctat
    20161 tcaaaaaatc ataccagatt ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat
    20221 agcagaatct tggccaaatt cactagatgt ttcttgtgcg caagaggaat ggggcttttc
    20281 gccgaaatac gacttagatg tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt
    20341 ggtagagacg gtataaaaca agaaccacat ccaaaaagtt ttttatactt ttgggattgg
    20401 ctatggtatg aaaggggaca agggatggaa gaatatcaag ccacgccagt aaaagaagta
    20461 aaaatagaaa atgaattaaa acgtacgatg ggatttttta ctgctttatc gactgttatg
    20521 ggcacagtga tcggtgcagg ggtatttttt aaagccgcaa gcgtagcaga agttacagga
    20581 tccgccagtt tgcatatgtt ttcttggttt ctcggtggta tgatttctgt ctgtgcagga
    20641 ctaacaggag ctgaactggc agctgctatt ccagaaacag gagggatgat taaatatatc
    20701 gaacggattt acggaaatac agcagctttt ttattgggct gggcgcaagt cgtgatttat
    20761 tttccagcaa atgtagcagc cttgtcgatc attttcggta cacagttcgt caatctattt
    20821 ggtctttccc agtcaatgat tgttcccgta gctgttacag ctgctgtatc gatcctgttg
    20881 atcaattttt taggttctaa agctggaggg gcttttcagt cgattacgct tgtctgcaag
    20941 cttattcctt tgtttgtgat cgtcattttc ggtttgttcc gacaagaagg agtagacttc
    21001 caactgtttc cgatccaagc aggagaaaat ctttcatttt tctcagcctt gggagcgggc
    21061 ttgctagcaa cgatgttcgc atacgacggt tggatacacg taggaaatat ttcgggagaa
    21121 ttgaaaaagc cggcgaaaga tttgccaaaa gcaatttctt taggaatcat cggaatcatg
    21181 atcgtttatt tattagtaaa tgcggtgttc ttaagaaccg catctatcga cggagtcgca
    21241 ggaaacagca atgcggcaag tgacgttgca aaaatgattt ttggcggctt cggtggaaga
    21301 ctggtcacag ttggtatctt gatttctgtt tatggaacca tcaatggcta tacccttact
    21361 ggaatgcgtc tcccttatgt tatggcaaaa gaaaacaacc tgccttttag caagctcttt
    21421 gctaaacttc atgataaaac taaagttcct gtagccgcag gtatcttaga gctagtcatc
    21481 gctattggca tgatgatgat cggtggtttt gatacactaa cggatatgct gatctttgtc
    21541 atttggattt tttatacgat ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct
    21601 gatttgtttc gaccatacaa agttccaatg tatccgttta ttccactagt agcaatcatt
    21661 ggagggactt ttatcgtatc aagcacgctt atcactcaga cctttttagc ttcgatgggg
    21721 atagtattga cacttgctgg tatcccgatt tatctctatt taaaacgtag atagctaaaa
    21781 cattcgaatt ttattagaac tgcacttctc tgccaaacaa aatattttat ttaaatgagg
    21841 agtacgtgga agaaatgtca aaagtactcg ttaattttga tcacgtaaat gtcgttttgt
    21901 ctacgatgaa atagcaaagt aaaagacaga aatcgggctc aaccgatttc tgtcttttac
    21961 tttgatttgc gtaactgaac tattcggcgt acgccgcgat aaatatttaa taaaaccaag
    22021 caaccagaaa tgaaaatggg aagccaaatc agatagacag ggtagccaag cactttagac
    22081 aaagcatgtg gagtcatttt atccatttca agaagtgctt gctgccaagt ctgaagattg
    22141 aagactagca tgaaacccga aataatcagg agaataaatc ctaaataatg aattgtagtt
    22201 ctttttctta cataagcgat cgtagcataa aggaacagta aaaaaatagg tacgccaaaa
    22261 acaaagtaca tattcagcat ctcgacaccc cctttgtgtt tatcttactt tactttgaaa
    22321 gaaatgagaa ataatagcct cgttacttgc ttgaaacagc aaaggactgc taagataaga
    22381 gagatacaaa gaatcaagga ggagtcatat ggcaagttat atcgaaccga ttcgttttgc
    22441 gctgattgtt tttccatttt tagccttagc gatctcatct gtttttttta tttatgaata
    22501 tcgaaagtat ggaacatttc tagtaacaag ggcagttatt ttgtattctt tcgtttttta
    22561 tttattgtgt gcgtattttt tggtgatctt accacttccg gcaaaggaga cggttgccca
    22621 aatgacaggg ccaaagatgg agctgcattt atttgcttca tggcaacatt tcagagacaa
    22681 cacggtattg gtcttgtctg aacctagtac ctacctgccg gcaatgaagc agagtgtatt
    22741 tttggaacca gcttttaatg ttttattact cataccattt ggcatttatt taagatatta
    22801 tttccggctt tcttttatga aaacgatgat tgctagtttc tgcctttctc tattttttga
    22861 attgacgcag ctaagtggac tttattttat ctacccaaga ccataccgct tatttgatgt
    22921 gaatgattta gtgacaaata cattgggtgg aatcattggt tttgtcgtta cgccgttatt
    22981 tacatttatg cttcctacaa gaagacgaat ggatgaactt tcttatgaaa aaggaaagac
    23041 tgtctcattg actcgacgct tggtcgctta tttgatcgac tggacatttt tatctcttat
    23101 taatttggta gcggcgattt tctttaaaat tgcgttcgat tcaggggcag ttacttctca
    23161 tatctggtgg cttttattag aagtaggggt atactttatg attgtaccgt ttataaccaa
    23221 tggatggaca cttggcaaaa aaattgtgcg tatccgtttg gtggaagaag accgcgaacg
    23281 aatttcttta aaagcattga tgatccgcta cgggtatctg tactttcttt tttatggttt
    23341 aagctggttg gggcagttgc ttcagcaaat catgaattcg acaacgaata cggtgttgca
    23401 gatgagtgca gtattcattt ctctcggtgt cttgttacta caaggtattt ttattttaaa
    23461 tatcttgcta tcatttttga gaaaaaaacg gattctgttt tatgaaaaag ctagtcacac
    23521 atatactgtt agcacgattg gccaaacaaa ggatgatgat tctcgaaatt agctgagcaa
    23581 aaacgctttg tctgtgttat aatgccaaag tcgtgataat ttaaagagaa gaggtgcacg
    23641 aatgaataat tttgatatat tccgcttcaa accgtttatc aatgaagcgt taaccgcaaa
    23701 gggcttcaaa cagcccacag aggtccagga acgtttgatt cctttaattg caaaaggaaa
    23761 aaatatcatt gggcaatctc aaactggtac aggaaaaaca catacgtttt tgttgccttt
    23821 gtttgaaaaa atcaatgcag aaaaagagga agtacaagtt gttattacag ctccaagtag
    23881 ggaactagct gcacagatct atgaagcagc tacacaaatc gctaaatttt ctacaccgga
    23941 gatccgtgtc tcgaattttg tcggcggtac agacaaacag cgtcaattaa atcgtctgca
    24001 tcaccaacag ccacaggttg tagtaggcac gcctggtaga atattagaca tgatgaatga
    24061 acaagcgctg aagattcaca ccgcatttgc atttgttgtc gatgaagcgg atatgacatt
    24121 ggatatgggt tttttaaatg aagtagacca aattgcaagt cgattaccag aaaagctgca
    24181 gtttttagtt ttttctgcca ctattccaga aaaacttcgt ccattcttac gaaaatacat
    24241 ggaaaatcca atcattgaag aaatcaaacc aaaagcagtt atttctgaag acattgaaaa
    24301 ttggctgatt tcaacaaaag gtaaagatga gaatcaaatg atttatcaac tgctgacaat
    24361 tggtcatcct tatttagcaa tcgtatttgc caatacaaaa cagcatgtgg atgaaattgc
    24421 cgattacttg aaaaaccaag gcttgaaagt agcaaagatt catggagaca tcaccccaag
    24481 agagagaaaa cgagtgatgc gacaagtaca gaatctggag tatcaatatg ttgtagcgac
    24541 tgatttagct gctagaggga tcgatatcga aggtgtctct catgttatta atgcagaaat
    24601 tccagaagat ctagacttct ttatccatcg tgtcggacgt actggacgta atggcttaaa
    24661 aggaacggcc attacgttat attcgccaaa tgatgagcaa gccattgatg cgatagaaaa
    24721 attgggcgtc tcatttgaac caaaagaaat caaaaacggg gaaatcgtca aaacatacga
    24781 tcgtaataga cgtgccaaac gagaaaaatc aaaagatgta ttagatccca cgctgattgg
    24841 tcttgtgaag aagaaaaaga aaaagatcaa gcctggttat aagaaaaaaa tcgattgggc
    24901 aattgcagaa aaaaataaaa aagaacgcaa aatcgaacgc agacagcaaa gccgtactgc
    24961 tcgtaaaaat aagaaaaatt cacataaata aaaaacaaga ggttgtgaca aaaggcgtgt
    25021 cacaatctct tgtttttaag ctagactttt tttcattccg atacaaagta cttcatcttc
    25081 aataaaaggt tctgtgcaag tatgataccc ttgtttttca taaaaatcta cagccgtcat
    25141 ttcagctact acataactag tctgacaacc ttctgaaacc gcccgttttt cagcagcttc
    25201 aagcaactga ccgcctaacc cttttcctcg gtattctttt gctacgcaaa atcgttccgg
    25261 cttgaagcaa gtaggtgttt tcttttcata acgcaaagta gctactggtc ttttttgatc
    25321 aagaaataga taataggttt gttgcgggat atcttttccg tcgaattcta gttctgcagg
    25381 aatcccttgt tctaatacaa aaacttgata tctcaaaaag cagctagctg cgcgtatcca
    25441 ttcatccgct ccaactattt ttttcatcat attcctcctt gtcatgaata gcagagatgt
    25501 attgattcct ttattcttat cgtaccaaaa atcgtggagt atgaccaaaa ggaaatgaaa
    25561 ataaaaattg agatattgac agtgatcaat agaatgcgta tactaaataa gtacgaaaag
    25621 gatatgattt gttttttaag actcacagaa aattctttct tggctgaaaa aagaatagtc
    25681 actaatcaaa ttgctccctt tttctatttt ggtagaaata ccgacgcagc tcagcgttat
    25741 cgagtttaag aggtaatgat tacacatcat tgcaatcaag gtggtaccgc gagttttcgt
    25801 ccttggctgt gatggtgttt ttttgtattt aatttataga aagcaggtaa tgacatgaaa
    25861 caattaagta gtgcacaagt tcgacaaatg tttcttgatt tcttccaatc taaaggacat
    25921 acgattgaac ctagtgcgtc tttgatcccc gtgaacgatc caaccttatt gtggatcaac
    25981 tctggtgttg caacattgaa aaaatatttt gatggttcag ttgtgccaga aaatccaaga
    26041 atcacaaatg cccaaaaatc tattcgaaca aatgatatcg aaaatgtcgg taaaacagca
    26101 cggcaccata cgatgtttga gatgctgggt aatttttcga ttggtgacta tttcaaaaat
    26161 gaagccatcc actgggcatg ggaatttttg actgatccaa aatggatggc attcgatcca
    26221 gaaaaacttt atgtaacagt ttatccaaaa gatacagaag caaaacgcat ctggcatgaa
    26281 gaagttggcc tgccactaga tcacattatt gatgtagaag acaacttctg ggatatcggt
    26341 gcaggcccat gtggtccgga tacggaaatt ttctatgacc gtggagaagc ttataacgat
    26401 ttagcagaag acgatcctga aaactatcca ggaggagaaa atgaacgtta tttagaaatc
    26461 tggaacttag tattttcaga attcaaccat aagcctgata acacatttga accacttcct
    26521 cataaaaaca ttgatacggg gatgggattg gaacgcatgg tctcaatcgt tcaagatgcg
    26581 tcaacgaact ttgagacaga cttgtttatg cctattatcc acgccgtaga aaagctaagc
    26641 aaacaagtaa aatatggtga aaacccagta acagatattt cttacaaagt aatcgcagat
    26701 catattcgcg ccctttcatt tgctattggc gacggtgcat tgccatcgaa tgaagggaga
    26761 gggtatgtct tacgccgttt gcttcgtcgt gctgtcatgc atgggaaaaa attaggtatt
    26821 gaacaagcat tcttatatcg tttagtccct gttgttgggg aaatcatggt aagttattat
    26881 cctgaagtac ttcaacaaaa agaatttatt gaaaaagtta tccgtacaga agaagaacgt
    26941 ttcaatgaaa caatcaatga aggtttggaa atcttaaatc aagtgatcca agaagtaaaa
    27001 aacgaaaacg ggacgactct agcaggaaaa gacatcttca aactatacga cacatatggc
    27061 ttcccggtag aattaacgga agaggtggca gaagatgaag gactgaaagt tgatcatgct
    27121 ggttttgaaa aagaaatgga tgcacaacgc gaacgtgctc gctctgcccg cagtactgaa
    27181 aaatcaatgg gggtacaatc tgctttattg acagatatca aagtagaaag taaattcatt
    27241 ggatacacag aacaagaatc agatagtcaa ttattggtca tcgttaaagg tgagtctctt
    27301 ttgaatgaag tcgcagaagg tgaagctcaa atgatctttg atcaaacacc tttctatgca
    27361 gaaatgggtg ggcaagtagc ggatcaagga tggatcatga atgaagtagg cgaaatcgta
    27421 gcagttgtta cagatgttca aaaagcgcct aatggtcaat ttatgcacac cgtagaagta
    27481 aaagcaccaa tgagtgaagg acaaacctat catctattga tcgatcaatc gatgcgcaat
    27541 cggatcatca aaaaccatac agcaacccat ctactgcaca aggcattgaa agaagtactt
    27601 ggcgagcatg caaatcaagc tggttcttta gtcgctcctg gacatttacg attcgacttt
    27661 acccactttg gtcaaatcac ttcagatgaa ttagctgaaa tggaaagaat cgtcaacgaa
    27721 aaaatctggg aagctatccc agttgaaaca attgaaacag atatcgatac agccaaaaac
    27781 atgggtgcaa tggctttgtt tggtgaaaaa tacggacatg acgttcgagt agtaaatatt
    27841 ggcggctggt caatcgaact gtgcggaggc acacatgtag caaatacaga aaacatcgga
    27901 atcttcaaaa tcgtttctga atcaggaatt ggtgcaggcg tgcgacgaat cgaggctgta
    27961 acaagtaaag aagcgtacga actacttgaa gaagaagaaa aacaactaaa acaagtagca
    28021 acaattgtta aatcacctca attgaaagaa gttgtttcaa aagcggaaca attgcaacag
    28081 caagtaagag agctacaaaa ggaaaatgaa caattagcta gcaagttagc gaatcaacaa
    28141 gctggtgatg tattcaaaga tccagaaacg atcaatggaa aaacaatcat tgcagcacaa
    28201 gtaaaagtga aagatatgaa tcagctgcgt caattagctg accaatggaa acaaaaaaat
    28261 atttcagata ttcttgtttt agcaaacgaa caagaaggaa aagtcagtct gctagcggca
    28321 atgacgaaag aatcaaatga agctggacta aaagctggcg acttgattaa agcaatcgca
    28381 ccgaaagtag gcggtggcgg tggtggacgt ccagacatgg cacaagctgg cgggaaaaat
    28441 cctgctggta ttccagatgc acttaaagct gcgaaagaat gggttgcgca acaatagggc
    28501 gtttacgttt gacagaaaaa caaaagttca aacattgaac gaaacatgat tatgggggcg
    28561 aagacaaaca agtcttgccc tcatactttt ttcaattgga tggcgcatct aatcaaatgg
    28621 tcggattcgg atggtcttta gttgacgaaa gtgacagaaa tttttacgat tagtataaat
    28681 aaaaaggggt gaaaaaatga atagtagaaa taagaccttt cgactagtct tacgtgcgat
    28741 cttgttagca atcatcatcg tacaagcgat ggttccttgg ctggggttta ttccattagg
    28801 attcattagt ttaacaatta tccatatcac agtaattatt gctgcagtag tattaggccc
    28861 aaaagatggc atggtgattg gcttgttttg gggaatcgct acaattgttc gtgcctatgc
    28921 catgccaaca acgccatttg atacattagt gttcacgaat ccgattatct cggtagttcc
    28981 cagagtactt gttggcttag tagcaggctt ggttttccac tggatttatc aaaaaaataa
    29041 atcaataacg atcagctcag cctttgcagg ggtactgggt tcattggtca atacagtctt
    29101 agtgttagga ttcatgggat tattttatac aggtgcaacg gcagatgcct atggtgtaga
    29161 ctctgctctt ttatttaaaa ccttagcagg ggttgcagca atcaatggga taccagaagc
    29221 aataggtgcg ggaatcataa caccattact agcaaaagcg ttatttgcag caacaccgtt
    29281 gaaaccagaa taatgaccat cttttaatag taataggacg cacgatagat agaaaaagga
    29341 agagaggaga ggaaaacaat cgtcctctcc atctttttac ctgagcaaca agcaaactag
    29401 aaggggtaaa aaacttggct aagaaacaag aagaaaaaaa gaaacaaata ccgcaacagc
    29461 ggacaaaaaa aggagtacta gcgggtatct taggattggt tacgaatctg atcttgtttg
    29521 tctctaagct aatgatcggg cttttttcag gaagtgtctc cattatggct gatgcagtga
    29581 acagtctatc agatactgct tcatccatat tgactttgat cggatttaaa attgctgcaa
    29641 agccagcaga tcaggaacat ccttatggac acgaacgctt cgaatatatc agtgggttat
    29701 ttgtttcgat catcattact tatgtaggat ttcaattttt agagtcttct gctgaaaaaa
    29761 tcatccatcc agaaaatgtc tctttaacac ccatcgtctt ttttgtccta atcatttcca
    29821 tctttttgaa atttgcacaa ggcaaaatgt atcaggttat tgcgcaatcc attgattctg
    29881 aaacattgag agctacagca acagatagtt ataatgatgt ctttactaca ctggctgtat
    29941 tgctttcagc agcaatcgaa tgggtaactg gatggcgtat tgatggatat ataggttttg
    30001 ctttagcatt atatatcctt tatagtggat tgacgatgct tcgaaatttt gtatacgaac
    30061 tattaggaag tcgcccaacg ccagcagaga ttgaagaaat ggaagagcat ttaaatgctt
    30121 gcgatgatat acttggctat catgatttat tggtgcataa ctatggaccc aataaacgct
    30181 ttgcatcagt ccacatcgaa gtagatgaat caatgaattt gaatgtggct cataagatta
    30241 ttgatctgat agaaaaagac tttaaaaagt ctttggatgt ggaacttgtc tgtcacttgg
    30301 atccaatgcc tgttgataac gaaaaatact ataatatctt gcggcagtta aagcaaattc
    30361 ttcttgaaac aggtacaggg cttaatattc atgattttag agtaattcat caaggaaaaa
    30421 cgctgcaatt tgatgtagtg gtccctgaga aattccagtt tgttgataca gaactagaaa
    30481 gaatcttacg tagtcaaata gaagaaaaaa tcggagatta ccaattggaa attacgtttg
    30541 atcacaatta tcttttatat taaatgagaa actataaaaa gactccttaa agtttttaat
    30601 ttaaagagtc tttttttctt ttaattgttt atggagagcc tgacgtgcaa gattatctgc
    30661 tcctttattt ttgttttcgg gcaaccattg caggatcaat aaagaaaatt cggatagaag
    30721 ttcttggatt ctttgaaggt agggaaggaa gagctcgttc ttcgttgcgt ttttgtcgac
    30781 cgtagaaact agtgttttgc tgtctgaaaa acagaaaatc gtttcattct gtaagtcgtt
    30841 tttctttaga aattctaaag tttttaaaaa gacttcgaac tctgcttgat ggtttgtccc
    30901 tatagaaagc gggatagata gctgttcttg tttaccttga tagacaatca agattccacc
    30961 tccactagga ccagggttgc cttttgtaga ggcatcaatg tatactttta acataaaaag
    31021 agattctcct ttttgttcaa actaatgtta tggtaagatt atatcattac tgaatcggtg
    31081 agaggttaga gaatgaaaaa aaatattcga tggcagcctg aactggcaca tacgatcatc
    31141 tattggtcat tgaccttgat cattctgttt tttagtctga ttctttcttt agaaaacaca
    31201 ggtccttatt ggaaaagcaa ttttgtttta ggtatttttc ttgtttttct atttctaggt
    31261 ttgcggcgtt cttttatttt aaaatcaaac tctgtgatta tacgttatgc tgctttttgg
    31321 aaagaccgag aaatatctgt cggacagatt cgatctttga cgccaactaa tactggggtg
    31381 caaatgatgt tgcaagatga gaaagcccca tctgttttca tgatgcgaaa aaaagatcaa
    31441 gtagtccttt ttgatcattt tgaaaaaaac ggacccaaat cagaatcaat tccttatcct
    31501 tatacgaaaa aaagaaaaag ctaattttaa aaatcagaac ctaaaaaaac gaatttttac
    31561 aggaaaagaa aagagtgaga cgtacggttc atcccgttta gcgtctcact ctatttgttt
    31621 atctattcat gcatactagt atcggtagtc tgaacgttgc ctttcttgac atgggcagct
    31681 tgtgttcctc gattcccttc gatgatctca aattcgacca cttgattttc ttccaaagtt
    31741 ttgaatccct cttcttcaat cgcagtaaaa tgtacaaaaa tttcttctgt gtcatcataa
    31801 ctgataaagc cgtatccttt tttgttgtca aaccatttta caattcctgt ggtcaaagca
    31861 ctcacgacct ttctaaagtc tgatcatagc cttcattata aagagtattt ataagtacgt
    31921 caaataaatt aaaaacctgt aattttatgt tttttttctg aataaaaaaa ggtataattg
    31981 gcatatctta acaaagaaga tattttgttg aataaagttc gtgaaaaggg gtaatcaaat
    32041 gaagaatgat ctgcaaattt cacaagaaac agagctagaa ccgattgttg aagtggctag
    32101 taaattagat cttaaggaag acgatttgga actgtatggg aaatataagg cgaaaatcaa
    32161 tttttctgga atcaatcgat caaaggataa cgctgagggg catttgatct tagttacttc
    32221 catcaatcca acaccagctg gcgaaggaaa gtcaacgatc actgtagggt taggagacgc
    32281 gctcaataaa atcaataaaa aatctgttat tgcattgcga gaaccctcac ttggtcctgt
    32341 gatgggaatc aaaggtggtg ctgcaggcgg aggatatgca caggtattgc ctatggaaga
    32401 tatcaacttg cattttactg gtgacatgca tgctatcaca acagctaata atgcattatc
    32461 tgctctttta gataatcata ttcatcaagg aaatgaattg aacatcgatg cccgccgagt
    32521 gatttggaaa cgggtagtcg atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt
    32581 gggtgggcca atccaaggtg ttccaagaga agatgggttt gatatcacag ttgccagcga
    32641 aatcatggct atcttgtgtc ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag
    32701 gatcgttgtc ggctatacat ttgaccgtca gcctgtgact gctggagact tgaaagcaga
    32761 aggcgctctg gcattactgc tgaaagatgc aatcaagccg aatcttgtac aaacaattta
    32821 cggaacacca gcttttgtcc acgggggacc gtttgccaat atcgctcatg gctgcaatag
    32881 tattttagct accaagacag cattgagatt agcagattac gttgtcacag aggcaggatt
    32941 tggtgcagat cttggcggag agaaattttt agatatcaaa gtacctaact tgaaaaaagc
    33001 gccggatgct gtggtgattg tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa
    33061 aaaagatgaa cttaaaaatg agaatcttga tgctttgaaa gtaggttttg caaatttaaa
    33121 gcgccatata cgtaatatgg aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt
    33181 cgtaacagat acagacaatg aactcacact gttggagcac ttgtgtgaaa atcaggggat
    33241 tcttgccaag agagcgagtg tatgggcaga tggagcagaa ggcggcgttg atttagcaaa
    33301 agctgtagta cgattgattg atagaaaaga agctaattac aagccattat accgcctaga
    33361 agaaacgata caagaaaaga cagaaacaat cgtcaagaaa atttacggag gaaatggtgt
    33421 tgttttcagc aaaaaagcgc aaaaacaaat agaagagttt acgaagaatg gatgggacaa
    33481 actgccgatt tgtatggcaa aaacacagta ttcattttct gatgatcctt cactgctagg
    33541 tgcaccggaa gatttcacca tcacgatccg cgaaatcatc ccaaaattag gggcaggatt
    33601 tttagtagcc ttgactggag atgttatgac gatgcctggt ttgcctaaaa aacctgctgc
    33661 attgaatatg gacgtgacaa atgacggaga agtgttggga ttgttttaag cttgattata
    33721 atctaccatc gaaggagctg tattttgact acagcttctt tttttagacg atagggaatt
    33781 aattttgttt acactaggat cattcttttt ctttattgta ttttgagcta tactaaagca
    33841 taataggagg gtatgatgaa aacgacattt ataagaaaaa ccggttttta cggtatgggt
    33901 agctcgattc agctaagaat caacaatgaa aaacgcagtg ttctcagtca tgaacaaaag
    33961 ataacgcttg atctggaagt gccctttacg atgcaggtca ccttttattg gctaaaaagc
    34021 cccgtttata cagtgaaaga agcaaagcct gcttatgtga tcacaatgaa ttacttgatt
    34081 cttcaaatct atccgttttt atttctagca actgggttgg gggccgtttt cctacaaaat
    34141 ttatttttca gtttttttgc agttattgtg ttgattgcgt tctttttcta tataaaaaac
    34201 cgggcatatt tgatcaagga ggaaaattat ggcaaactct gatctttttt taatcagttt
    34261 caatcgaatc gaaaaatgga tgagagacga gatgggaaat gcacgaaata tgggatttac
    34321 cgaacttgtc agacgcctag ctcaaagaaa acaattaatg atcagaaaat acgaagatga
    34381 tcttttacag cttgctcagt taaggaatgc gatcgtccat gatcggatcg cagtcgattt
    34441 tgtcattgca gagccaaatg attggacagt caagcgaatc cagctgatcg aacaagaact
    34501 gattcagcct gaaaccgtgc tgcctcgatt tgcaaaacgt gtaacaggtt ttgaacaaga
    34561 tcttccgtta cttgaactgt taaagatagt agcagaaaaa aggtattcac aatttccgtt
    34621 atacaataaa ggaaaatttg tagcattgat tactttacgt aatatcgggt tttggctagc
    34681 aaaagaaagc caaaaaggtc cggttgattt aacaaataaa cgagcgttgg atttgatcat
    34741 ccaaaacgga aagtacacaa attatcattt tgttccagct aaaacacata tttacgaggt
    34801 tgaagcgatg tttcgtgagc aagggacttt ggaagccgta cttatcacta aagatgggaa
    34861 tcccgatggt aatctgctcg ggatcgtacg acctcgtgat atttataaac aaatagaaaa
    34921 ggattgattt tttgttaatt atttattgga tattcagcgc tttgctagta ggagtggatc
    34981 agttggtcaa atggtggatc actgataatc tagcgcttgg agagacgaaa aatcttatac
    35041 caggtatttt gtcactgaat catattcgaa acactggagc cgcatggagt atgctagaag
    35101 ggaagatgtg gttctttact atagtgacac tcattgcagt cgttgtgatt ctcacattga
    35161 tgattaaaaa tcgtgaaaaa ggtaatcgct ggttcatgat tggattgagt ttgattctag
    35221 ctggtgcgat tggcaatttt atcgaccgta ttcgtcttgg ctatgtagta gatatgttcc
    35281 agacagattt tatgagcttt ccgattttta acgtagcaga tgtgacactt gtatgtggag
    35341 tgatttgtat gttaatttat attattttag atgaaaagga tcaaagaaaa aaatgacaga
    35401 acttaaaacg acgattcacc atgaatctgg taggatcgat aaagtattga ctaatttatt
    35461 caccgattac agtcgttcgc aaattcagct gtggctgaaa aatggagcgg tttctgtcaa
    35521 tggacagcca gtcaaagcaa attacaaagt aaaacaagga gacgagatta ctgtcttgat
    35581 ccccgaacca gaaacattag atgtggtccc agaaaatatc ccgttgacga tcgtttacga
    35641 agatgaagat gtagcggtga taaacaaacc tcaaggaatg gtcgttcatc cttcagcagg
    35701 tcatacgcat ggcacaatgg tcaatgcgct gatgtaccat atcaaggatc tttctacgat
    35761 caacggcgtt attcgtccag gtattgttca ccgaatcgac aaagatactt ccggactgct
    35821 aatggttgcc aaaaatgacc aggcacacga atcattagct cagcaattaa aagataagac
    35881 ctctttgcgc aaatatgttg ctttagtcca tggagagatt cctcacgaaa aaggaaccat
    35941 caatgcgcca atcggccgtt cgaaggaaaa ccggaaaatg caggcgataa gagaagatgg
    36001 gaagtcggct gtcacacatt ttactgtatt ggaacgtttc gaaggattta ctctggttga
    36061 attaaaacta gaaaccggaa gaacacacca gatccgtgtg catatgaagt atatcggtta
    36121 tccgttagct ggagatccag tatacggacc gaagaaaact ttgaaaggca atggtcagtt
    36181 tctccatgcg caaatgttag gttttacgca cccgactaca aacgaaaaga tgatctttga
    36241 agcacctctt ccagaagttt ttgaaaaaac tttagaaaaa ttaagaaaaa acattgcatt
    36301 ttaatttaga taacggtata gtaattaaca acgaaagcat aaaacttaat agaattcctt
    36361 taagtttagt cccgtgaggc taagaagggt gggagcaagg atacgtatgg tgcgccataa
    36421 gtagattgct cattgacata gtatagctat cagtgtatca gtatacgctc agtctaaaca
    36481 ttcctcctgt cctcttaggg caggagtttt tttatgcaaa aaagtagggg gaatgaaaat
    36541 gcaagcaaaa gaagtagtag atcaggtaac catgaagcga gcattgactc gaattactta
    36601 tgagattatc gagagaaacc atagtatcca agatatcgta ttggtaggta tcaaaacacg
    36661 aggaatctac attgcatcaa gaattgccga acgcctgaag cagctagaag atatcgatat
    36721 tcctgtagga gaactggata tcactttata ccgagatgac aaaaaagaaa atccagaaga
    36781 accggaactt cattcttctg acatcccagt ttccttagaa ggaaaagaag ttattttgat
    36841 tgatgatgtt ttgtatacag gacgaacgat tcgagcagca atggacgcag taatggattt
    36901 tggcagacca agaaaaattt cattagcagt cctggtcgat cgtgggcatc gagaattgcc
    36961 aatccgtgca gattatgttg ggaagaacat tccaacatct cgggcggaag agattttagt
    37021 agagatgcaa gagcttgatg gacaagatcg catcatgatt ttgaaagagg aagattagag
    37081 gagagagaaa catgtcggag aaaaaaggat ttcataatga tgacgtggtg ttagatatcc
    37141 acgatcgccc aaccccagga cattgggtag gattaagttt acaacacttg ttcacgatgt
    37201 tcggagcaac cgtgttagtc cccatcttag taggtatcga tccaggtatt gcgctagtca
    37261 gttcaggact aggtacattg gtgtacttat ttactactaa aggaaagata cctgcgtact
    37321 taggaagcag cttcgctttc attgcagcga tgcagctttt gatgaaaaca gatggttatc
    37381 cagcgattgc acaaggagca gtcacaactg gattagtcta tttgattgtt gcgctgatca
    37441 tcaaaaagat cggttcagca tggctggata aaatcttgcc tccaatcgtc gtcggtccag
    37501 tcatcatggt tatcgggttg ggacttgcgg caaatgcagc aacaaatgct atgtataatc
    37561 agggcacgta tgatttcaag tatattgctg tagcgctgat cacattagga ttaacaattt
    37621 tcttcaacat gtggctaaga ggttttctcg gattgattcc gattttacta ggtattatta
    37681 gcggatacat cgtagccttg ctgtttggaa tcgtggatac taagccaatc gtagacgctg
    37741 cctggtttgc tgttcctaac tttgagattc cctttgtaca atatgaacca aagctatatc
    37801 ttggagcaat cacaacaatg gcacccatcg cttttgtgac gatgacagag catatcggcc
    37861 atcttatggt attgaataaa ctaactaaac gtaatttctt tgaagaaccg ggtcttcata
    37921 agacgttgac aggtgatgga ttagcacaaa tcgtggcagc ctttgtcgga ggtcctcctg
    37981 taacaagtta cggtgaaaac attggtgttc tggctatcac acgtgtccat agcgtgtttg
    38041 tgatcggtgg agctgcagta tttgcagtca ttttaggatt cgtaggaaaa ttaagcgctt
    38101 tgattttaag tatccctgga ccagtcatct caggaatcag ttttatctta tttggagtaa
    38161 ttgccgcaag tggaatgaag attttaatcg agaacaaaat cgattttgat aaaaagaaaa
    38221 atctgttgat tgcttcagtt atcttagtag taggaattgg cggactcgtc tttgaagtag
    38281 gcacatttac attgtcggca atggcattag caactgtact tggcatcatt ttaaatctta
    38341 tcttaccaga aacttcacga agcgaagaaa actaaggagg aaacattatg atcatcaaat
    38401 cagaacgtat cagcttaaag catttattga ccgttgaggc acttactgat caagaagtta
    38461 tgggcttgat ccgcagaggg caggaattca agcaaggtgc taattggact ccgcaaaagc
    38521 gccagtactt tgctacaaac ctctttttcg aaaatagtac tcgcacacat aaaagcttcg
    38581 atgtagcgga gaaaaaacta gggctagaag tgattgaatt tgaagcaagc acaagctctg
    38641 tccaaaaagg ggaaacacta tacgatacag tactgacgat gtcagcatta ggcgtagacg
    38701 tagcggttat ccgtcatggt gatgagaact attatgacga actgatacaa agtaaaacaa
    38761 tccagtgttc gattatcaat ggaggagacg gaagcggaca gcatccaacc caatgcttgc
    38821 ttgatttgat gaccatttat gaagaatttg gtcattttga aggtctgaaa gttgccatcg
    38881 taggtgatat cactcactct cgcgtagcta aatccaatat gcagatgtta aaacgtctag
    38941 gcgcacaagt attcttctca ggtcctagag aatggtacga cgatgaattc gaagtgtacg
    39001 gacattatat gccattggat gaactactag atcaagttga tgtcatgatg atgcttcggg
    39061 tgcaacacga acgccatgat ggaaaagaaa gtttctcaaa agaaggctac catcaggaac
    39121 atgggttaac agttgagcgt gcgaagaagt tgcaaaaaca tgcaatcatt atgcacccag
    39181 caccagttaa ccgtgatgta gaattagctg attcattagt ggaaggatta cagtcgcgga
    39241 tcgttcagca aatgagcaat ggagtattcg tcagaatggc aatcttagaa gcagtgctaa
    39301 atggaaaagc gtaattatag gaggatgaaa aaatgaaaac actcattaaa aacggtcaga
    39361 tcaatacacg aaaaaatatg acgacaccag cagaaatttg gatagaagat gggcgaatca
    39421 aagcaatcgg tacaggattc tcagaagccg aatttgatga agtgtttgat gcaaagggac
    39481 aattgatcac acctggttta gtggacgtcc atgttcattt gagagagcca ggatttacat
    39541 acaaggagac gatcgaagct gggactcgct cagctgctcg tggcggtttc actactgtat
    39601 gcgcgatgcc gaatttaaat cctgtacctg atacggcaga aaagctgaga caagtatatg
    39661 acatcattag aaaagatgca gttgtgaagg tattgcagta tgcgccaatc acagaaaatc
    39721 tgcgaagtga gaaattagtt gatcaagaag cactgattga agaaggtgct tttgctttca
    39781 caaatgacgg tgtaggtgtc caaacagctg ggacgatgta tctggctatg aaagaagctg
    39841 caaaaaacaa taaagcccta gtagcccata cagaagatga atctttatta tttggcggcg
    39901 ttatgcacgc tggcaagaag gcagaagaac ttggtcttcc cgggatctta agcgtaaccg
    39961 agtcttctca aattgctaga gatctgttgc tagctgaagc aactggtgtt cattaccatg
    40021 tttgccatgt ttctactaaa gaaagtgtcc gtgtaatcag agacgcaaaa aaagcaggaa
    40081 tccacgtaac agcagaagtt tcaccccatc atcttatttt gatcgacgaa gatattcccg
    40141 aagatttcgg cttttggaaa atgaacccgc cgctaagagg acgggaggac agagaagctt
    40201 tgatcgaagg cttgcttgac ggtacgatcg attgcatcgc tacagaccat gccccccatg
    40261 gattagaaga aaaaagccaa agtttcatga aatcaccatt tggtatcgta ggaagcgaaa
    40321 cagcgtttca actgatctat actcattttg tagaaactgg tcgattcacg ttggaacaag
    40381 tgatcaattg gttggcagtt aaaccggctg agatttttgg tttgaatgca ggaacgctaa
    40441 cggttggtgc ccccgctgat gtagcagttt ttgatattac acaaacttgc acgatcgata
    40501 aagaagactt tttatcaaaa ggggagaata caccttttat cggctggaaa gtaaaaggcg
    40561 aaacacaaat gacttttgtc aacggaaaac ttgtgtggca gaagggagaa taaaagtatg
    40621 gaacgtttgc ttatattaga agatggaaca gttttcgaag gaaaggcttt tggtgcagat
    40681 gctagtgtct tcggagaaat cgtttttaca acaagtatga ctggttatca agaaactatc
    40741 actgatcaaa gttttaacgg tcaaatcatt acatttacct atcctatggt gggtaattat
    40801 ggggtaaata gagatgatta tgaatcgatt tctccaacat gcaaaggagt agtagtaaaa
    40861 gaacatgcac gtgtagcttc aaactggcga aaccaaatga cgctagattc atttttgaaa
    40921 caaaaaggaa ttcctggaat atcaggtatc gatacgcgcg cccttactcg caaaatccgt
    40981 gaagtcggaa cgatgaaagc tagtatagtt gatgctggag attccttcga acatgcattt
    41041 gatcagctga aagcaaccgt tctagcaacc aatcaagtgc agcaagtctc aactaacaaa
    41101 ccttatccaa gtccaggaac aggtcggaaa gtcattgtca ttgacttcgg cttgaaacat
    41161 agtattttac gagaattgag taaaagaaac tgtaacctca cagtacttcc ctataacaca
    41221 gatgccgaga cgattttagc gctatcacca gacggagtca tgctgacgaa tggacctggt
    41281 gatccaaaag atgtgccaga agcaatcgaa atgatcaaaa aaatccaagg aaaagtaccg
    41341 atttttggta tttgtctcgg tcaccagtta tttgctttag ccaacggtgc agatacctat
    41401 aaaatgaaat tcggacatcg aggattaaat cacccggtac gagaagtagc gaccggaaga
    41461 attgatttta cttctcaaaa tcatggctat gcagtcgatg aagccacgat tgatccagaa
    41521 aaactcatcg taactcatgt agaagtaaat gacggaacag tagaaggagt caaacacaga
    41581 gattatcccg ctttcagtgt gcagtttcat ccagatgctg cccctggtcc gcatgatgca
    41641 ctacatttat ttgatgaatt tatggaaatg atggacgcag gaaaggagca gtaaacagat
    41701 gccgaaacgt acagatatca agaaaattat ggtgattgga tctggtccga tcatcattgg
    41761 tcaagccgca gaatttgact acgcaggaac acaagcctgc cttgccttaa aagaagaagg
    41821 atatgaagtg gttttagtaa attctaatcc tgcgacgatc atgacggata aagaagtggc
    41881 agatcaagta tatatcgaac cgatcacact tgaatttgta tcaaggattc ttcgaaaaga
    41941 gcgtccagat gctctccttc caactttagg cggacagaca ggcctcaata tggcaatgga
    42001 gctggcagca tcaggtattt tagaagaatt gaacatagaa cttctaggaa caaagctttc
    42061 tgcgatcgat caagcagaag atcgtgatct attcaaacag ttgatgaaag aattgaatca
    42121 gccaatcccc gagtctgaaa tcgtcaatac agtagaagaa gctgtagatt ttgcaaataa
    42181 aatcggctat cctgtcatcg ttcgtccagc ctttacttta ggcggaactg gtggaggtat
    42241 gtgtgaaaat gaagaagaat tacgtatcgt atcagaaaat ggattaagtc tttcccccgc
    42301 tacccaatgt ttgattgaac gaagtattgc aggttttaaa gaaattgaat atgaagtgat
    42361 gagagattcg gctgataatg caatcgttgt ctgcaatatg gaaaattttg acccggtggg
    42421 tatccataca ggtgattcta tcgtatttgc ccctagtcaa acattgtctg accatgaata
    42481 tcaaatgctt cgtgatgctt ctctttcgat tattcgagca cttaagatcg agggaggctg
    42541 taatgtccaa ctcgctcttg atccacatag ttttaattat tatgtgattg aagtcaatcc
    42601 gcgtgtttct cgttcatctg cgttagcaag taaggcgact ggctatccga tcgctaagct
    42661 agctgctaaa atagcgcttg gattaacatt agacgaaatg aaaaatcctg ttacaggaac
    42721 gacttttgca gaatttgaac ctgcattaga ttatgttgta gctaaaattc ctcgctggcc
    42781 gtttgataag tttgaaaacg gagaacggct tttaggcacg cagatgaaag caactggtga
    42841 agtgatggct atcggtcgga atatcgaaga gtctttattg aaagctgtgc gttctttaga
    42901 aatcggtaca caccatatcg aacttcctga gttggcagac atcacggacg atgacctaat
    42961 ccaaaaagtg gttcgagcac aagatgatcg cctgttttat gtagctgaag ctattagaag
    43021 agggtacacc atagaagaat tagcggattt gacaaaaatc gatttattct ttttagataa
    43081 actgttgcat atcgtagaaa tcgaacagca gttgaaacag cagccgttgg atgacaagat
    43141 attgaaaaca gcgaaacaaa acggttttac agacaaaaaa gtggcggagc tgtgggaaat
    43201 aaacgaagaa gccgttcgcc agatgagaaa agaaaatgat ttgattcctg tatataagat
    43261 ggttgacact tgtgcagcgg aattcgaatc aaaaacacct tatttttata gttcctatga
    43321 atatgaaaat gaaagttcac gatcagaaaa gccctcaatt ctagtactgg gatctggtcc
    43381 tatccgtatc ggacaagggg ttgaatttga ctatgcgact gtgcattctg tcaaagcaat
    43441 ccagcaggca ggatacgaag caatcatcat gaacagcaat cctgaaacag tttcgactga
    43501 cttttctatt tcggataaat tatattttga gccattgacc ttcgaagatg tgatgaatgt
    43561 gatcgagcta gaacagccga taggagtaat cgttcaattt ggtggtcaga cagcaatcaa
    43621 cttagccgaa cctttgactg ctgctggtgt aaaaatctta gggacaacga tcgaagactt
    43681 ggatcgtgcg gaaaatcgtg atttattcga gcaagcgctg aaagagttgg atattcctca
    43741 acctttaggg gatactgcta caagtaaaga agaagccgtg gttatcgctt cgagaattgg
    43801 ttatccagtt cttgttcgtc caagctatgt attaggcgga cgtgcgatgg aaatcgttga
    43861 aaaccagcga gatttagaag attacatgga acatgctgtg aaagcatctc cagaacatcc
    43921 agtactagtg gaccgttact tgcttggtgg tgaatgcgaa gtggatgcga tttgtgacgg
    43981 tgaaacagta ctcatcccag ggatcatgga gcatattgag cgtgctggtg tccattcagg
    44041 ggattcaatg gctgtgtatc cacctcaaac attatctgaa tcgatcaaga aaacgatcga
    44101 agattacacg atccgcttag cattaggttt gaattgtgtc gggatgatga atatccagtt
    44161 tgtcatccat gaaaatgagg tttatgtgat cgaagtcaat cctcgagcaa gtcggactgt
    44221 tccattctta agtaaggtga caggaattcc aatggcacaa attgcgacga aagctatttt
    44281 gggagagaaa ctaaagacac ttggttatga aaacggactt tatccagaaa caaaagaagt
    44341 tcatgttaaa gcacctgtct tttcttttac gaaattgcag aaagttgata cttacttagg
    44401 gcctgaaatg aaatcgactg gagaagtaat gggatctgac agacaattag acaaagcttt
    44461 gtacaaggca ttcgaagctt ctggattaca cttgcctgaa tatggagccg tattatttac
    44521 gattgcggac gaaacaaaag aagaagcact agaattagct aaacgtttta cagaaattgg
    44581 ctatagtctg ataggaacga aacatacagc tgcttatttc gaaaaaaatg gtctggtcgt
    44641 tgaaacagta gcgaaaattt ctgaggaagc agcagaaaaa aatgtagtcg acttaataag
    44701 agatggtaaa acacaagtgg ttgtcaatac gattgataaa gatcggggaa atgcttctaa
    44761 agacggtttc atcatccgaa gagaagcagt tgaacatggt attccattgt ttacttcttt
    44821 agacacagca gatgcgatcg ttcgagtgat ggaatcccgt gctttttcaa ctaaccctat
    44881 ttaacggcga gcacatgtta agacaaaaag acgaaaaacc gttgcaggag gggacagatg
    44941 aaacaagaaa tgatggaaat cgttcagcaa aaacagctgg caccaaagat ttttgaaatg
    45001 acattgaaag gtgatctagt aaaagaaatg cagcaaccag gacagttctt gcatattctc
    45061 gtgccgcgtg cagacttgct attacgccga ccgattagct tgaatcatat cgaccacgaa
    45121 acaaatactt gccgtatcat ttatcgggtg gaaggtgagg ggactagagt cttctctact
    45181 ctttctgccg gagaccattt agatgtcatg ggtcctttag gcaatggttt tgatttgtcc
    45241 aatcttcata caggagacga agtatttata atcggtggag gaatcggtgt ccctcctcta
    45301 tatgagttat caaaacagtt agtcgaaaaa ggaattaagc caacgcattt tttaggtttt
    45361 gccacacatg aagtcgttta ttatgaaaaa gaatttaatc gtctaggaga aacaagaatc
    45421 gctactgacg atggaacata cggtatgcac ggaaatgtcg gtaatttatt gttaggaacg
    45481 aagacggaac ctacagctgt ttttgcatgt ggttcaaacg ggttattgaa gacagtggaa
    45541 cagctgtttt cttcgcatca aaatgtccag ttatcattag aatcccgcat ggcatgtggg
    45601 ataggggctt gctatgcttg cgtttgtcat acgccggaag atgaaacagg aacaaaaagt
    45661 gtgaaagttt gtgacgaagg accgattttt aaaataggag aggtaattat atgaatgaga
    45721 atcctttagc ggtcaaactt cctggcctag aattaaaaaa tcctatcatg ccggcaagcg
    45781 gctgtttcgg ttttggcaaa gaatacggaa aatactatga tttgaatcag cttggatcga
    45841 tcatggtcaa ggcaacgact ccaaatgccc gctttggcaa tcccacgcca agagtggcgg
    45901 aaacaccatc tggcatgttg aatgcaattg gtttgcaaaa tcctggtcta gacgttgtta
    45961 tgcataaact tcttccagaa ttagaggagt acaatgagtt acccatcatc gcaaatgttg
    46021 ctggtgcttg tgaagaagac tatgtagaag tttgtagcaa aattggcgaa gcaccaaatg
    46081 tgaaagcaat cgagctgaac atttcttgcc caaatgtcaa acatggcgga attgcttttg
    46141 gaacagattc tgatgttgct tttcaattaa cacaagcagt aaagaaagtt tcttctgttc
    46201 ctgtctatgt caaattgtcc ccaaatgtaa cagacatcgt tccaatcgct caggcaatcg
    46261 aagctggagg ggcagatggg tttacgatga tcaatactct tcttggtatg cggattgacc
    46321 ttaagacaag aaaaccgatt ttagccaatc aaacaggtgg actctccgga ccagcaatca
    46381 agcctgtcgc tattcgtttg attcaccaag tagcaagtat atccagcctt ccaatcattg
    46441 ggatgggcgg cgtgcagaca gtggatgatg ttcttgagat gtttatggct ggagcaagtg
    46501 ctgtagctat agggaccgcg aatttcaccg atccgtatat ctgtcctaaa ctaattaaag
    46561 agctccctgt gcgaatgagc gaactaggca tcgaatcgct ggaaagatta atcaaagaag
    46621 tgagagagga acgtgaacga tgaatcaact agaccaacga ccaattattg cattagattt
    46681 ttcaactagg caagaggtag aagactttct tcgatttttc ccgaaagaag aaaaattgtt
    46741 tgtgaaaatc ggaatggaat tgttctacca agaaggacct gaaatcgtac gttatctaaa
    46801 agaggctggt cataatgtat ttttagatct gaaacttcac gatattccga atacggtcga
    46861 aaaagcaatg cggggattgg caaagcttgg tgttgatctc acctgtgttc atgccgctgg
    46921 cggtattcgt atgatggagg cggcaatgcg aggattagaa gaggggactc cagaaggtgg
    46981 aaaacgtccg ctacttctag ctatcactca gcttacttct acaagtgaag aagaaatgca
    47041 tgccgatcaa ctaatcgaag tttctttaga aaaaagtgtg atccattatg ctagctgtgc
    47101 caaaaaagca ggattagatg gtgtggtttc ttctgcttgg gaagtagaag cgatcaaaga
    47161 aatggcagga gacgaatttg tttgcttaac tccgggtata agaccagaag gaacagtcgc
    47221 aggagatcag acaagagtag tcacaccttc tcaggcaaaa aagatcggct caacttttat
    47281 cgttataggt cgaccaatca ctcagtcaac taatccttat aaagcatatc aaacgattca
    47341 aacagaatgg agctaaccta aaatgaatgt tgaacaaagt atcgcaaaag acttattaga
    47401 aattgaagca gtatttttaa atccaagtaa tccttttaca tgggcaagtg gaatcaaaag
    47461 cccaatttac tgtgataacc gaatcacgat gagctatcca aaagtccgaa aagagatcgc
    47521 aaaaggatta gcaagcaaaa tcaaagaagc ttaccctgaa gtacaagtga ttgctggaac
    47581 tgcaacagca ggaatccctc atgccgcatg ggtagcggaa atactggatt tgccaatggt
    47641 ctatattcga agtaaggcga aagatcatgg gaaaggtaac caaatcgaag gacgtatatt
    47701 tgaaggtcag aagatggttg tgatcgagga tttgatttct acaggcggaa gtgtcttgga
    47761 agcagctgaa gcagcaaaaa gagaaggtgc ggatatcctt ggtgttgcgg ctatctttac
    47821 gtatgagttg ccaaaaggaa aagcaaattt cgaaaaagcc gaaatccctc ttatgacgtt
    47881 gaccaattat tctgtattga ttgaagcagc tttagaagat cgttatatcg atgagcaaga
    47941 attaactctg ctaaaagaat ggaaaaaaga tcctgaaaat tggcagattt aagcgcgctt
    48001 gatgaagata gagaaatccg atttgtgtct ccagaatcat caattggaat agcaaaaatc
    48061 caaaaactga ttgcgagaag catcttttca tcaggtatga aaagaaactt ctatcgtgat
    48121 cagtttttga tttttttaaa attcattttt ctaagactgg ttagcagaaa gaagagaata
    48181 atctcttctc ttttttctat tatatataga ttttctctat tttttggtaa gggagctgtt
    48241 tcaaaaatgg taaagagcgg aaatgttgcg aatatcagtt atttaatgat atgatattcg
    48301 gaaaaagaaa tctcaaaggg aggtaacttt tatggggttg aatgaattta ttttaggatt
    48361 gaacaattta gagacgatca caagggcacc tggatttttt aaatatacag agcatacagt
    48421 tgcagcacat tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc
    48481 gaatggacaa ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc atgactacac
    48541 agagcgtttt attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg
    48601 gatgctgcaa acagttgaag aaaagatgac ggacgaattc atcaaccaag agattccagc
    48661 tgagttccaa gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg
    48721 agaactatta gccatttcag ataaggttga ccttctttat gaatcttttg atgaaatcat
    48781 caaaaataac ccggaaaaag tttataagga gatgttcatt gaggcagtga ctacgattaa
    48841 aagctttgaa catcggcctt ctgttcagta tttctttaaa gagatatttc cggaactatt
    48901 gaaccaggaa ttttatggaa aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa
    48961 agaagaaaaa taaaaatgat atgagatgca gggggaagta tagttgaaaa agaatatgga
    49021 tgtcgaatgg ggctattctg gagacaaagg tccggaaaat tgggcgagtt tatgtgattg
    49081 gtttgcgaga ggtgcggaat ttccattgca gtcgcctatt catttgacca agggggaaga
    49141 aaccaaaata gaaggagaca cgctttcctt tcattatcaa aagcaacggt ttactgacaa
    49201 ggaattcaaa aacacgattc atttagtacc ttttgaccaa ctcagttatg tagagttcaa
    49261 gaaagaacgc tattatttga cagatattca tttccacttg ccttctgaac atatcatcaa
    49321 cggaaaacag gaagatattg aatttcattt tgttcatatg aacagcaaaa aagagaatct
    49381 agttgtgggt gtcatgtacc agctaatggt acaggaaggt tggctgtata atcaagaaaa
    49441 tggggaaagt tggaacttag aaaaacatga gcattgggta aatccggatg tgttctttcc
    49501 ggagcaaaaa agccatttcc attatattgg ctcattgaca actccgccta ccagcgggcc
    49561 aatccaatgg tttgtaatgg atcatgtagg aaaactgaac caagagtttc ttctcccctt
    49621 cgatggtcag tatgctcgac cgaacaaccg accaatccag cccttgaatg gaagagaaat
    49681 ttattatttc gaggagtttg agcaatgaat attcagcaga tgaaatacgt ggtcgctgta
    49741 gcaaacaatg gtagtttcag agaagcggca aagaaaatgt ttgtctccca gccaagtctt
    49801 tcagctggta tcaaagaatt agaaaccgag ttagggatca cgctgtttac tcgaacgaat
    49861 cgaggggcat atttaacaga agaagggcaa gacttcttaa atcgtgccga gaaaatattg
    49921 gtccagcttg atacattaga aactcactat ctaacgagtg aaaagagaga caatttttcg
    49981 atcgctgccc agcattatga tttcttaggt cctttgaccg cacagctact cgaggaattc
    50041 aaagacgaga tcaaacaatt tcggatcatg gagacgacaa cagcgagagt gatcgaagaa
    50101 gtcaaagagc agcacagtga gatcggcatt atttatatga atgagcataa tcaagctggt
    50161 atcagaagat atttacaaca aggagagcta agtggccgca gcattggaaa attcaaaacg
    50221 catatttttt taaggcaagg acatccatta gctgaacacg cagagatcac caaagaagaa
    50281 ttgttatctt atcctcaagt tcgttttaca caggatggaa ataatttccc gtatttttat
    50341 gaagacctta tcgagatccc tgatcaagaa acagtgattt atacgagtga tcgaggtact
    50401 ttgatgaata tcgttcttga aactgatgct tacgcttcag gatctggtat tgtgattgga
    50461 gggattcgcg agcacttgcg gttaatccct ttggcagaag gcgaactaaa tgaattttat
    50521 atcatttatt cggcgaaacg tccgctcagt gaaatcgctc aaagatttat caaagagctt
    50581 actcggttac tggaccagca gagctaagaa aagcagatga gaacgctgtt tattgaacac
    50641 cgttgatcaa aaaagagggg ttgtgacaga attttgtcac aactcctctt tttgtagcaa
    50701 acaatttata actaattgct gttgtgggaa agtacgcaaa tatgattcta gaaacactca
    50761 ttgattacct gttttgtttc atagctgtaa ttttttcttc ttctggtgtc acgataatcg
    50821 tcttttggtt ttcataaata acataaccag gcttggcgcc atttggtttt cggatatgct
    50881 ttacctgtac aagatcaacc ggcacttggg cagagtatcg gtattttgaa aaataggcag
    50941 ctaactcggc agcttctgta attgtttcat ctgagggctt gtcactttta atgatgacgt
    51001 gcgaaccggg tatatttttt gcatgaagcc aatagtcggt ttttttagct gtcttcattg
    51061 tcagctggtc attttgaaga ttgtttttac caacaagaat gagcgttccg tcagaagaaa
    51121 agtattgatc aggctgagat ggtttcttac gtttttgttt ttttaagttc ttctttttca
    51181 ggtatccttc tgaagtcaat tcttctttaa ttgcttcgat atccattggg ccggcaattt
    51241 ccagttgaga taagacagat tcaagatatt ggatttcatc tttggcttct tggatttgtt
    51301 cgccaatcag cttaacagcg ttcttcagct tttggtatcg atggaaatat ttctgtgcat
    51361 tttgatttgg tgtcaatgcc ggatctagtg ctattttgat tggccgatct tcttcataat
    51421 aattaggtaa aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta
    51481 gttcgccatt tctacggtaa ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt
    51541 ttttcaattt atttttattt cgttttagtt cattttcgat ttttcgaatc agttctccgc
    51601 cttgttgttt tgcccgatct ttctctgctt tttcgtgata atagctatct aataattgac
    51661 tgagtgttgg ataagctgtt acagcagatg cctgttcgga caatacctga taagcaatcg
    51721 gtgtgaaaaa atctttattt tccgtttcgt aaaaagtagg aataggctga tggctgatgg
    51781 ctgagaaaaa ttcgtcccat acaaccatct tttcattagg acgttccagt aatcttttgg
    51841 tcagctcttg agcgatatcg aaaccgattc cctgaaactg acgttggatt gcttttggat
    51901 ctagttcagt ttgggaaagc cgctgaaaga ttttttcttt ctcggagcta aatgggttca
    51961 agacctcttg ttttggcgga gcaacatatt ctacaccagg aagcaaagaa cgataagtgt
    52021 tttgagagct tccaatgtgt ttgatcgcat ccagtatttt tccagtttct cgattaacta
    52081 agacgatcgt actatgacgt cccattaatt caacgatcaa tatgatattt tgtagatcac
    52141 ccaactcgtt tcgtttagca aagtggaaat ggatcactcg atcattttcg atttgttcga
    52201 tgtcttctaa aatagcgcca tccaaatatt ttcgaagcat catcacgaag ttaggcggat
    52261 tatcaggatt ctgataatcg atttgggtaa tctgtacacg agcataactt gggtgcgcag
    52321 ataataataa tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt tcattttcat
    52381 aaggctgatg gattttcgag atccggccag atagtaacgt ttcacgcaat tcgttgacca
    52441 tcgcgtgggt aaatacacca tcaaaggaca tggttcattc ctcatttctt ttcataagta
    52501 tattcattat aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa agcaaaagtc
    52561 tagaaaaatc ttggactaat tacataaaaa acagtgtttc tatcgttgaa atccaaattt
    52621 ttaggtataa taaaaaatag aagaaaaagt agaaaagaag gtgtttgtta tttgggaagt
    52681 agttaaatcg agagtacaag aaatgaatga acatttctca gtgtcattcg cagaaaatcc
    52741 aagtaagtat tttcgacgtc ttgtttttct gctcatctta tcattgctgt taacagttgc
    52801 aatgttttta ttgccggaaa aaatccgaga agagcaagta aatagtctcg tttattcgtc
    52861 cataccaacc agcaaattga aaccttttga ttacagcaaa gctgatcaga aaatcaaaca
    52921 aagccgggcg atatcggtga tgttcagcaa accgaacggg aaaacctatc aggacatgct
    52981 tgatgttttt aatgatcctg agcttatgga agaattgaat cgaccgatct tttattatcc
    53041 gatcgtttat gatgtacatg aactggaaca aaaatataac atacgaacgg atgaagtgac
    53101 atttattttc tttgatggag gaaaagaagc aaatcggata actgcgggta agggaggaat
    53161 aactgatttt gataaggagt tgatccctga actgaatcga cttccgttag ccaatatcaa
    53221 acaattagaa gaagaattgg atagaacggg agtaacttcc caaaattccg agattgctaa
    53281 ttgagacatt ggcgcgatga aacggtgggt ctatatagcg ctatttttaa ttatcagaac
    53341 aagtgatgtt tgatcttcaa aataacaaat aaaaaattaa aaaatatagt tgacgaaatt
    53401 caaaaattcg attagactta aatcaattct taaggaaagg gtgacctgta atgaacttat
    53461 caaaacgtat ttcagcagaa aacaaaaaaa attggcgcag ctggcgtaga taagttgtat
    53521 taatggtgag actttcccat agatacaact ttttagagtg catttctaaa gcttgtatct
    53581 atgctagaca tgaacattga ctgccgcata gattagaatc tatgcggttt tttgtgtaaa
    53641 gacaatgacc gctttggcca ttgtcttttt ttgtagactg gatacttgcc tgattataat
    53701 aaataaaaag attgactttg caggaggaaa aaaatgaaaa acaaacgatt aataacagtt
    53761 gtagtaatta ttttagttta tttagcaggt gcatttattt atggaaaagc ctcaggcgaa
    53821 aaagaaagta cttcgacgaa ccagacaaaa aaaacaacag ttgttggggt attgcaatac
    53881 gtgagccatc cggcattaga tgagatttat cgaggaatca aagatggtct gaaagaagaa
    53941 ggattaaaag aaggagaaaa tcttgagatc aaatttcaaa atggacaagc cgatcagagc
    54001 aagctagcca caatgagtca acagctagta caatctgatc cagatgcctt gattggaatc
    54061 gcaacacctg ccgctcaatc attagcaaac acgacttcat cgatcccagt tgtgttaggt
    54121 gctgtaacgg atcctgtggg ggctggatta gtcgcttctt tagagaaacc tggtggtaat
    54181 gtaacaggtg tttctgatca gccgcctgtt acttcacaaa tcaagcttgc ttctgatttg
    54241 ttgccaaatg caaaaaaagt cggaatcttg tatgcttctt cagaggataa ttcaaaatac
    54301 caagtagcac aagcagaaga agctgcaaaa aaagaagggt tagaatcagt aaaatatgcg
    54361 gtgccttcca ctaatgagat tgcacaaacg gttcaagtga tgagccgaca agtcgatttt
    54421 atctatgttc cattagataa taccattgcc aatgcgatgc aaacagtggt aaaagaagca
    54481 aacaaagcaa atatccccgt cattccatct gtggatacaa tggtggaaca aggtggacta
    54541 gcaacaatcg ggatcaatca atatcaatta gggcttcaaa gcggtaagat ggcggcagaa
    54601 cttgcgagtg gaaaagaaaa accggaaacg acgcctgttt atacatttga tcaaggggat
    54661 acagtgatca atcaatctca agctgatcgc ttaggaatca ttgttcccca atcgatgaag
    54721 gaaaaagcca aaatcattac agatgactcg cagcaagaaa catctaagga ggatgacaaa
    54781 tgatcgtatc agctatttca caaggcatgc tttgggcaat attaggatta gggattttca
    54841 tgacttatcg gatcttagac tttcctgata tgacgacaga agggtccttc ccattaggtg
    54901 ctgctgtctg tgtgacagct attactcatg gggtagcgcc gattgtagct acattattag
    54961 gcgtaggtgc tggcatgata gcaggactcg ttacggggtt actttataca aaaggaaaga
    55021 taccggttat tttagcaggg attttagtga tgtcaggatt gaactcagtt attttgtttg
    55081 tgatgcgaac accaaatctt tctttattga atcatccgat tctacaagac agcttccatc
    55141 gctttgggtt acctgattac tttgacacag tccttcttgg cttcatctct ttgggcatct
    55201 tgattgctgt actgctattc ttcttcaata cagatttggg tcaaggctat attgcgacag
    55261 gagacaacga aacgatggct cggtcgttag ggataaaaac cgatcgtatg aaaattttag
    55321 gtttaacttt gtcgaacggt gtcattgcat tatccggtgc gctgattgcc cagaacgacg
    55381 gatatgcaga tgtaaataaa gggattggcg taatcgtcat cggtcttgct tccatcatca
    55441 tcggtgaagt gatcttccaa gaattgacgc tagcagaaag attgattgct attgttgtcg
    55501 gcagtatcat ttatcagcta ttgattttag tagtaatcaa actagggttt gacacgactt
    55561 atctgaagtt gttctccgct atcatcttag ctatttgtct gatgatcccg caattgaaga
    55621 aagcactgaa cttaaaaacg ggttttgaga aggaggcata gtaatgactg atatattgac
    55681 aatcaaaaat ggcagaaaag tcgtaaacaa tggaatgaat gaagagaaag tactattgga
    55741 tgaaattaac cttacaatca gagaagggga cttcatcact gtccttggcg ggaatggtgc
    55801 tggaaaaagc acactgttca atacgatcgc tggtacgctt caactcagtc aaggatcgat
    55861 ctcttttaaa gatcagctga tcacaaaaga ttcagaagaa aaaagagcac gattcctttc
    55921 tcgagttttc caagacccca agatgggaac ggctcctcgg atgacagtag cagaaaattt
    55981 gctacttgca caaaaaagag gtcaaaaacg gaaattccgt ttacgtaact tgaaaaatca
    56041 aaaagaagat tttttcagac tatgtcaaga agtcggtaat ggcctagagc aacacttgga
    56101 tactccagcg ggtgaattat ctggtggtca acgccaagca ttaagcttgc tgatggcgac
    56161 gatcacagct cctgagttac tattattaga tgaacatact gcagctttag atcctaaaac
    56221 agctaaagca ttaatgcgat taacaaatca gcggatcaat gaacaaaaat tgacttgtct
    56281 catgatcacg catcgaatgg aagacgcctt gaaatacgga aatcgtctga tcgtactcca
    56341 aaaagggaaa atcgttcaag atatttctga aaaagaaaag caacatttga caatgggcga
    56401 ccttttgcag ttctttgaag aagtagtgga ttgatttcga cagaaaaatt cctttcagta
    56461 gaaggacttg aaaactcatt gacatcttcc tttgattcat cctatactta aaacaataaa
    56521 caattaaaca tatttgggtg ttttgtagga tcacgtagtc aatgatttta acaattaaat
    56581 agaatattct ttgctctgac aagtaaacga tgagaaaaga atttgctact ttgccaccta
    56641 gacgtttcgt actttttcgt atcaaacgct aagtggcttt ttttattgaa gtaagaacaa
    56701 aggaggaata agatgatttc tggacaaacc aaattagctg ggctctttgc tgatccggct
    56761 tctcacagtt tatccccatt gatgcacaat actgcttttg aagcaaacaa catcgatgcg
    56821 gtgtatttag ctttccaagt aaatccaatg aatttgaagc aagcagtcga aagtattcga
    56881 acttttgata tgttaggtgt aaatctttcg atgccgaata aaacagcggt tattccttat
    56941 ctggatgagg taagccagga agcgcaattg atcggagcag tgaatacgat cgtgcacgaa
    57001 aagggtcgtt tgattggcta taatactgat ggtgcgggct tcatgcaatc cgtgcaagaa
    57061 gctgggattt cgatcaaaaa gaaaaaaatg actctactgg gagctggagg tgcagcaaag
    57121 gcagtcactg ttcaagcagc gttagacggc gctgaggaaa tcgtagttta taaacgaaac
    57181 aaccaaacat tcgctcaagt aaaagaaaca ttcgaacaac tcagctatcg aacttcttgt
    57241 aagataaaag ttcgtgactt tgccgatacg catcaattaa gaaaagactt gaaagaaagc
    57301 tgtcttctag tcaatgcaac agatatcggg atgggaaaga aaatcaatca gtcaccgctc
    57361 ttagatataa gcatgatgca tgagaatcta gcggtttttg atttgattta tgcacctaga
    57421 gaaacacgtt tattaaaaga agcaaaaaaa gtaggagcaa aaggatacaa tggacttggc
    57481 atgttgatct accaaggagc aatcgcgttt gaattatgga caaaggaaaa gatgccgatc
    57541 catgtgattg aaaagctgtt tgattaaaaa aggataatag ataaagatct tttattctcg
    57601 cataaacggt ctcaagaaaa gtaatccttt atgtatcgaa gcatttattt cgttattttt
    57661 tattaaatta tcaaaaaatg ataggctttt gtcgattggt tatcgtatcc tttatttatc
    57721 agtaagaaac gaggaggaac cacaatgagt cggatggatc gctataagga catccataag
    57781 aaggcgaaac ctctgaagcg ggatacgggg agtatttttg ctagaagaga acgaaaacga
    57841 acgcgtatgg aaaatgagga agatgctacc cgtatttatg aaagagagca gcattcattt
    57901 gaacatttga accaatacga gcaacagata tacagagatg caccaagagc agaagaacaa
    57961 caaaaaaata aaaaattttt caatcggcaa aagaagaaca aaccgttaaa acctcgaaga
    58021 aagcgatcgt ggaaaaaaat cattctatcg attttgcttg ttttgatcct ttattcaggt
    58081 atctcatttt ttattggtaa atatgtagct gaacatgaca caagcttaga ggcagcgact
    58141 tcagaaacct ttcatgggca aaaagcgacg aatggtgcgc ataatatttt gatccttgga
    58201 agtgatacac gcggagaaga tgccgggcga gccgatacga tcatggtctt acagttggat
    58261 ggtccttccc ataagccgaa actgatttct tttatgcgtg acagcttcgt tacgatccca
    58321 ggggtaggac aaaataagat caattcagct tatgcatacg gaggagccga tctagtgcgt
    58381 caaactttag tagaaaattt tggaatcgat tgtcaatact atgcgaaagt cgatttcaaa
    58441 tcgtttgaaa aagtgatcga tgccttattc atgaacggag tgaagatcga tgcagaaaaa
    58501 gatctgaatc tggatggtgt agatattaaa aaagggggcc agaagatgga tggacatgtt
    58561 cttttgcagt acgcacgttt ccgaatggat gaacaaggag actttggccg tgtcagacgc
    58621 caacagcaag taatgaacgc tattttcagc caattgaaga atccattgaa tctgatccgt
    58681 gcaccttatg cagctggtaa agctttagga tatacatcca cagatgtcac tgcttggttc
    58741 ttgacgaaaa acctcttttc gattgcacga ggagctggtg gcattgatcg aataagtgtg
    58801 ccagtagacg gttcgtggaa ttatgggcaa agtgattatg ctggcagtat tcttgaaatt
    58861 gataatcaaa tgaatcaatc tgctattaca gattttttga ataaatgatc ataaaaaaga
    58921 tcaagaagaa gctgcgcaag gcttttgtca tgaatcaatc aaacgattca ctcgtaaaaa
    58981 acgctttctt atggtagatt taaccaagag aggagctgtt tacattggat atctatctga
    59041 acttcaaaaa tgagtcgcgt gaagccatcg ctttttatga aaaagttttt cagactgaag
    59101 catcaggtat caccacatac ggtgaaatgc ctggaataga acaaatgaac ttgccagaag
    59161 aagcaaaaaa tctaatcacg aatgcacggc tgaacattta tgatacatta gttatgtttg
    59221 cagatacgcc agaaggaatt ggtcctgaat tagttgtggg aaataatgtt tctttagtca
    59281 tccagacaag aaatgaagaa gaaacgaagc gcctatttga aagcttagct gagggtggta
    59341 agattgagat gccactggat gctgttccgt gggctcagtt gtatgggatg gtaactgatc
    59401 gatttggcat caactggcag attaaccaag agtgacagct aaagatgaat attttctgga
    59461 aacatcaagc agcaaacgga tcatcgtttg tttgcttgat gtttttgtgt ctatttcgac
    59521 tatacggtat ctttttttta ttgccagatg cgaaaatatg ttccataata gagaagtccg
    59581 caaaaaatta agtaacggag gtcagaagat gaagacggtg atactagctg aaaaaccttc
    59641 acaagcaaaa gcctatgccg aaaccttcca acagcaaaag cggcatgaag ggtattatga
    59701 aatcagcgat ccattgtttg ctggtgaagt aaccatcact tatggttttg gacatttggt
    59761 agatatggtt gctccaggag cttatgaaga gcgttgggca aaatggtctt tagaaaattt
    59821 gcctattttc ccagaaacct tccgttacga agttccaatg gacaaacgtg cacaattttc
    59881 gatcgtaaaa cgagaattgc agtcagccga taccatcatt attgcaacag atggtgatcg
    59941 agaaggtgaa gccatcgcgt ggtcgattat tcgacaagca aacgcatttt ctaaggaaaa
    60001 gaactattta cgtttatgga tcaattctct tgaaaaagaa gcaatctatg aaggttttaa
    60061 gaatctccaa cctggaaaga actattttcc aaaatataaa gaagcgcaag caaggcagaa
    60121 cgctgattgg ctgattggaa tgaatggaag tccattatat agcttgctac ttcaacgaaa
    60181 agggattgat ggcagttttt ctcttggtcg tgtacaaaca ccgacactct atatgattta
    60241 tcagctgcaa gaagaaatca aacacttcaa aaaagaaacg tactttgaag gagaaggttt
    60301 aattacgaca aacaaagggc agttttctgg aaaaatcgta ccaaaaaaag catttaaaac
    60361 ccaagaagag ttaacgaagg aaatagaaaa acttggagcg catttaggta agcaaaacgg
    60421 acggattctt gaagttacga aaaaggaaaa gaggttgcat agtccacgtt tattttcgct
    60481 atctagtttg cagacaaaga tgaatcagct aatgaaagca agtgccaaag ctacacttga
    60541 tgctgctcaa ggattgtacg aaagcaagtt tctaagctat ccaagaacgg attcctttta
    60601 cattacagaa aatgagcatc agtatctggt taaacatctg caaaattata aaaaattttt
    60661 aggaatagaa gaagtacaga caactgagat caaagcaaag aaacgatatg tcgatgcgaa
    60721 aaaagttcaa gaacatcatg caattattcc gacaaagacg attccgactc ctgcacgctt
    60781 tgcgaagctt tccaaattac agcaagcaat ctatttgcaa gttttgcgca ctactgtggc
    60841 tatgtttgct gcagattata tctacgaaga gacgaccgta atgacgggcg tacagcaatt
    60901 aaagctaaaa agcatcggaa agatcccatt acagcaaggt tggcaggaaa tcttacgcat
    60961 caagtcaaaa aagaaagaag ctcagactct tcctcctatc gtagaaaatg aagaagcaga
    61021 agtagaactg aaaagtatgg agaaagaaac acaacctcca aaaccctata ccgaaggaac
    61081 gttgatcaca gcaatgaaaa ctgcgggaaa aacgctggat aatgatgaag cgcaagaaat
    61141 tctcaaagaa gtagaaggaa tagggacaga agctacacga gccaatatca ttgaaaatct
    61201 taagcagaag cattacatcg aagtcaataa aaacgaaata actgttacgc caaaaggaat
    61261 cactctttgt aaggcagtag ccaatgaacc attactgacg agcgcagaaa tgactgcgcg
    61321 ctgggaaggg tacttgagaa aaattggtaa acaagagggg acaccatctg tttttttaga
    61381 gaatatcaaa aaattcatcc tacatttatt ggcagaagtt cctaatcaaa tcgacaaggt
    61441 agacttttca gatgagatta gcgggacacg tcagataaaa gcgatggaga agaaaaacga
    61501 tcaattagga agatgtccaa aatgtaaaaa gggaatagtc atgctttatc ctaaaatcgc
    61561 tacatgcttg aatccggaat gcgacttcaa attgtggcca acagtagcta aaaagaagct
    61621 gactaaaacg aatctcagag atttgctttc aaaaggaaag acaagtaaag ttgtcaaagg
    61681 ttttactggt aaaaaaggga aattcgatgc agttttagta ttaaaagaag atatgacaat
    61741 cggtttttct tttccatgga tcgaaaacag tggaacaaaa gcagttgatg gaaacaaaga
    61801 aacatcaaga aaaaataatg aaaacgaagt agagtaaatg aaaacaatga aatagcggaa
    61861 tatttattcc gaaaagtgag aatataaata ccaataaaac taaaaaatat cagtgaatca
    61921 agcaaaaacg ttggggtagg ctgattttat atagatagca aggagttttt ttatttgaaa
    61981 aaagtgcatt catttttaaa ttatcttatg ggagcaatca cgattgtggc tgtgtaccaa
    62041 gcatttctaa agtatcctga ttacgggacg aagttgttgt cattgacgca caatcaaaca
    62101 gcatattcac caacgttgat tttatcgact ttgactgcag gagctaaaag cgctacttat
    62161 ttttttcata aaacgacaag catgctaaag cgaaagaaac agcgaaaaca attgttggtg
    62221 gtggtagcgg ctatgatcat tatttatttt ctaccagtta ttacacaagt atttcatcaa
    62281 tattttggtt aagaatacga gagtttgtga caaaagtcat gtcacaaact tttttctttg
    62341 tcattttttc gtaagaaaaa gcaaaataaa agtatataca ttgattaaaa gtatatactt
    62401 ggatataatg tacttactat agaatgagaa tggtatagag ggtggcgaaa tatgaaaaaa
    62461 atgaaaaagt ataaaattag aaaatctggc aatagcgatg taacaactat cccaccagaa
    62521 gtaaaagaat tcatgggtgt gcagacaggt gacgcaatca gttatgtttt tcaatctgat
    62581 ggttctgtac gaatgatcaa agcacaagaa gaacctgata ttgattcatt agttgattcg
    62641 atcatgaatc aatatgaaga cgcgttaaaa gatttggttg atttatgagg tatctttcga
    62701 ctaaagaaat tatcaaaatc aacgcgaaag ttattttacg tcactcgcca ggcgaaatga
    62761 tcggtgtaaa agatgcaaat gcattagata tggccgtcaa acagccttca caagcagtat
    62821 tcaatcaaga actctatcct gaaatctatg aaaaagcggc gattttagca attaatttag
    62881 ctaaaaaaca tccttttcat aatggcaata aacgaactgc tcttgttgcc atgttgttgt
    62941 ttcttcaatt gaataattgt ccagttgctt tttctcgtca agaagctgtt gattttatca
    63001 taatgattac cactagttca ttggatttcg attcactaaa gtccaagata accagttatt
    63061 tacgacaaac cgcaataaaa tgaacgaatt aaaaacttta tttctcaatt agatcagcat
    63121 gtcattaagg aatatgctga tttttttatg tttaatcata caaaatagtt ttttcataaa
    63181 aatatggaaa atatggatta attttgaaat aattgattta aaaggaatac aaaagagtga
    63241 atagtaggaa atagtggttt agaactagtg ggctgatttt ttatatattt tagtttacat
    63301 aatatatatt atacaaagta gatttaaaaa atgataaacc tctctcgaac ggttttttat
    63361 caaaaatcgt catttttcca tcaaaatctg cttatgcaaa att
//
LOCUS       DACEPD010000004        62051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.004, whole genome shotgun sequence.
ACCESSION   DACEPD010000004 DACEPD010000000
VERSION     DACEPD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.004"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(8..616)
                     /locus_tag="IW797_000223"
     CDS             complement(8..616)
                     /locus_tag="IW797_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223028.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLITAYSEKVDKKVVADRLAEWKKTNEQVKKTTVSSQK
                     NESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(775..2172)
                     /locus_tag="IW797_000224"
     CDS             complement(775..2172)
                     /locus_tag="IW797_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1223029.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2204..3175)
                     /locus_tag="IW797_000225"
     CDS             complement(2204..3175)
                     /locus_tag="IW797_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1223030.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3549..3974
                     /locus_tag="IW797_000226"
     CDS             3549..3974
                     /locus_tag="IW797_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223031.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(4462..5163)
                     /locus_tag="IW797_000227"
     CDS             complement(4462..5163)
                     /locus_tag="IW797_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1223032.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5179..5619)
                     /locus_tag="IW797_000228"
     CDS             complement(5179..5619)
                     /locus_tag="IW797_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1223033.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5619..6194)
                     /locus_tag="IW797_000229"
     CDS             complement(5619..6194)
                     /locus_tag="IW797_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ1223034.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(7042..7521)
                     /locus_tag="IW797_000230"
                     /pseudo
     CDS             complement(7042..7521)
                     /locus_tag="IW797_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(7680..8381)
                     /locus_tag="IW797_000231"
     CDS             complement(7680..8381)
                     /locus_tag="IW797_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1223035.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(8489..9898)
                     /locus_tag="IW797_000232"
     CDS             complement(8489..9898)
                     /locus_tag="IW797_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ1223036.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(9946..11811)
                     /locus_tag="IW797_000233"
     CDS             complement(9946..11811)
                     /locus_tag="IW797_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1223037.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAEADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(11932..12783)
                     /locus_tag="IW797_000234"
     CDS             complement(11932..12783)
                     /locus_tag="IW797_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1223038.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(13093..13806)
                     /locus_tag="IW797_000235"
     CDS             complement(13093..13806)
                     /locus_tag="IW797_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1223039.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(13874..14200)
                     /locus_tag="IW797_000236"
     CDS             complement(13874..14200)
                     /locus_tag="IW797_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1223040.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(14323..14550)
                     /locus_tag="IW797_000237"
     CDS             complement(14323..14550)
                     /locus_tag="IW797_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1223041.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(14872..16125)
                     /locus_tag="IW797_000238"
     CDS             complement(14872..16125)
                     /locus_tag="IW797_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1223042.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            16186..16926
                     /locus_tag="IW797_000239"
     CDS             16186..16926
                     /locus_tag="IW797_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1223043.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(17012..17770)
                     /locus_tag="IW797_000240"
     CDS             complement(17012..17770)
                     /locus_tag="IW797_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1223044.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(17767..18795)
                     /locus_tag="IW797_000241"
                     /pseudo
     CDS             complement(17767..18795)
                     /locus_tag="IW797_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(18815..21982)
                     /locus_tag="IW797_000242"
     CDS             complement(18815..21982)
                     /locus_tag="IW797_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223045.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(22206..23090)
                     /locus_tag="IW797_000243"
     CDS             complement(22206..23090)
                     /locus_tag="IW797_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1223046.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(23185..23622)
                     /locus_tag="IW797_000244"
     CDS             complement(23185..23622)
                     /locus_tag="IW797_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223047.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(23839..24750)
                     /locus_tag="IW797_000245"
     CDS             complement(23839..24750)
                     /locus_tag="IW797_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1223048.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(25207..25635)
                     /locus_tag="IW797_000246"
     CDS             complement(25207..25635)
                     /locus_tag="IW797_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223049.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(25663..26301)
                     /locus_tag="IW797_000247"
     CDS             complement(25663..26301)
                     /locus_tag="IW797_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1223050.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(26305..28341)
                     /locus_tag="IW797_000248"
     CDS             complement(26305..28341)
                     /locus_tag="IW797_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ1223051.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            28467..29132
                     /locus_tag="IW797_000249"
     CDS             28467..29132
                     /locus_tag="IW797_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1223052.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(31067..34216)
                     /locus_tag="IW797_000250"
     CDS             complement(31067..34216)
                     /locus_tag="IW797_000250"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ1223053.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(34229..35428)
                     /locus_tag="IW797_000251"
     CDS             complement(34229..35428)
                     /locus_tag="IW797_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ1223054.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(35418..37013)
                     /locus_tag="IW797_000252"
     CDS             complement(35418..37013)
                     /locus_tag="IW797_000252"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1223055.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(37597..38304)
                     /locus_tag="IW797_000253"
     CDS             complement(37597..38304)
                     /locus_tag="IW797_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223056.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(38369..39205)
                     /locus_tag="IW797_000254"
     CDS             complement(38369..39205)
                     /locus_tag="IW797_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1223057.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(39223..40632)
                     /locus_tag="IW797_000255"
     CDS             complement(39223..40632)
                     /locus_tag="IW797_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1223058.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(40646..42469)
                     /locus_tag="IW797_000256"
     CDS             complement(40646..42469)
                     /locus_tag="IW797_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1223059.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(43312..43638)
                     /locus_tag="IW797_000257"
     CDS             complement(43312..43638)
                     /locus_tag="IW797_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223060.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(43684..44046)
                     /locus_tag="IW797_000258"
     CDS             complement(43684..44046)
                     /locus_tag="IW797_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ1223061.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(44083..44298)
                     /locus_tag="IW797_000259"
     CDS             complement(44083..44298)
                     /locus_tag="IW797_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1223062.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            44425..44760
                     /locus_tag="IW797_000260"
     CDS             44425..44760
                     /locus_tag="IW797_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1223063.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(44836..46833)
                     /locus_tag="IW797_000261"
     CDS             complement(44836..46833)
                     /locus_tag="IW797_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223064.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(46931..47206)
                     /locus_tag="IW797_000262"
     CDS             complement(46931..47206)
                     /locus_tag="IW797_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1223065.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            47438..48172
                     /locus_tag="IW797_000263"
     CDS             47438..48172
                     /locus_tag="IW797_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1223066.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            48211..49353
                     /locus_tag="IW797_000264"
     CDS             48211..49353
                     /locus_tag="IW797_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1223067.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(49448..50839)
                     /gene="sufB"
                     /locus_tag="IW797_000265"
     CDS             complement(49448..50839)
                     /gene="sufB"
                     /locus_tag="IW797_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ1223068.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(50844..51314)
                     /locus_tag="IW797_000266"
     CDS             complement(50844..51314)
                     /locus_tag="IW797_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ1223069.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(51301..52536)
                     /locus_tag="IW797_000267"
     CDS             complement(51301..52536)
                     /locus_tag="IW797_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1223070.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(52533..53819)
                     /gene="sufD"
                     /locus_tag="IW797_000268"
     CDS             complement(52533..53819)
                     /gene="sufD"
                     /locus_tag="IW797_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ1223071.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(53832..54605)
                     /gene="sufC"
                     /locus_tag="IW797_000269"
     CDS             complement(53832..54605)
                     /gene="sufC"
                     /locus_tag="IW797_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ1223072.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(54802..55650)
                     /locus_tag="IW797_000270"
     CDS             complement(54802..55650)
                     /locus_tag="IW797_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1223073.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(55674..56360)
                     /locus_tag="IW797_000271"
     CDS             complement(55674..56360)
                     /locus_tag="IW797_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1223074.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(56353..57387)
                     /locus_tag="IW797_000272"
     CDS             complement(56353..57387)
                     /locus_tag="IW797_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1223075.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(57782..58120)
                     /locus_tag="IW797_000273"
     CDS             complement(57782..58120)
                     /locus_tag="IW797_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ1223076.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(58122..58478)
                     /locus_tag="IW797_000274"
     CDS             complement(58122..58478)
                     /locus_tag="IW797_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ1223077.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(58484..59671)
                     /locus_tag="IW797_000275"
     CDS             complement(58484..59671)
                     /locus_tag="IW797_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1223078.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(59862..60707)
                     /locus_tag="IW797_000276"
     CDS             complement(59862..60707)
                     /locus_tag="IW797_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1223079.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            60941..61906
                     /locus_tag="IW797_000277"
     CDS             60941..61906
                     /locus_tag="IW797_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ1223080.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT        18064 a        12880 c        10256 g        20851 t
ORIGIN      
        1 ctgtttttca ttgtccatag gattgatatt gtccttgtga tgactcattc gtaataattg
       61 aggcttgatt ttcttcactt cccgttatcg tttgtccact gccagaatct gaaggacttg
      121 ttcctgtcga ctcgtttttt tgagaagaga cagttgtttt cttgacttgt tcatttgtct
      181 ttttccattc cgctaaacga tcggcgacca cttttttgtc tactttttca gaataagcgg
      241 tgatcaaacg ggctactttg tcgccatcat gagtcaaaag caaaacggtc aaatgcttgt
      301 ctttaaattg atattgtctg agagaaatcg cactaagtac gtcatcgata ttaataaatt
      361 gattttcttc ttttcctttt ggcaaataaa tgtccaattc gcctttttcg taattggcag
      421 ttattttata ctccgcccac caataagttg agaattcttt tttgaaatta tctactgatt
      481 ctttgcttgc tacgattttt tctgtttctt tctgagcaag atctgctgtc atttttcctg
      541 aaaatgctgc ggataaaata agcagtaaga aaaaaccgaa taaccaaaag agaagaaacg
      601 gtcttttttt cctcatgctt tcctccactc ctatttaatt ttgactattc tattatatac
      661 aaaaaatgaa atagaaagac aaaaaagttg aaaaagatcg taaataattg aacttctttt
      721 tcacaaaatg gtaagaatgg agaatttcac gcatttgctc tcaactattc tttcttaagc
      781 ctcaaatccg tttttatctg atacgtcttt gaaccaatga cccgattttt tgattgttct
      841 ttttccatct tgtgctaagt ctactgaaat aaagccatat cgattcttat atgcattggt
      901 ccacgaccag ttatccatac atgtccacat atgatagcct aaacaattgg caccttcttg
      961 aatggcttga tggacatatt tcagatgaga ttggacaaat tcgatccgat aatcatcgtg
     1021 gatcatgcca tcttctttga taaatcgtgc ttcgttttct acacccatac cgttttcaga
     1081 aataaagcag tcgatatttc cataatgatc tcgtacattg atcaaaatat catagattcc
     1141 ttgttcatag atctcccatc cgcgataagg attcatttta cgaccaggca tttcataatt
     1201 gtcgaaataa tcctcaggca ttggaccgtt tttagtatcg attggtgttt cttttgcttt
     1261 tacccggcgt ggctggtaat aattgatacc taaaagatct actgtatttt ctttgataac
     1321 cgtcaaatcc tctgcatgga tcacaggcat gagatcaagt tcttttacta tctcaatcaa
     1381 ttcacttgga aactcgcctt ttacagctgg atcaaggaat gaacgattga agaacgcatc
     1441 tgctatttgc gctgctttaa catcttcagg gtttgattcg tctcttggat agcttggtgt
     1501 cagattcaag atgataccaa taacaccacc ttgctccatt tcatggtaag atttgatggc
     1561 taacgctgaa gccaacgctt catgatagcc aacttggatc gcttttttca tatctgactc
     1621 ttctggataa tgccatcgat ataaataacc tgcttcgact ggaacgattg gttcattatg
     1681 agtaaaccat ttttttactt tcgaaccaaa caattcaaaa caacttttgg catatttttc
     1741 ataggcttca accgtttctc ggttcaacca tccgcctttt tcttgcagat acatcggcat
     1801 atcgaaatga tacagattca caaagggttc gataccgttt tcaatcaact catcaaaata
     1861 acgatgatag aaacgaacgg cttcttcgtt gattgttcca gattcatcag gtatcaaacg
     1921 gctccattgg atagatgttc gaaatgaatt atgcccagtc tgtttcatca actgtacatc
     1981 ctcttgatac ttcgtataaa cttgcgaggt tttttcaggt cctacttgat caaagaactt
     2041 ttgcggttct tttttgtacc aataatcaaa aatgttctct ccttttccat cgcctgaaat
     2101 ccttccttct gtttgtgggc cacttgctgc agaaccccac caaaaatttg ctggaaattg
     2161 atattttttc atcaaagcac ctctttttct tttttagttc gttttagttg attccatgta
     2221 tgatattttc catcacaatc actttttcat tttcttcctc ttcttttttg attcgtttta
     2281 acagctgttc tgctgctcgt tccccgattt tatctgttgg tggcgtgatc gtgattttcg
     2341 gaaggatact cagatcgttt ttatctaaat aatcatagtg actgaacgca atatccttcc
     2401 cgattctgac accttcttga ttgaaacatt cgatggcacc aatcgacata ttgtaattgc
     2461 tgacaaaaat agcagttgcc cctttttcta ataatgcttt tgccccgtaa tagccgcttt
     2521 ttctagaata gtctcctgta aagacctcta attggatcga tggatcattt tggaccgctt
     2581 ctttggctcc tgctaaacgt tctcttgcta cgtagtctgt ttgagtggca gcaataaatc
     2641 cgattttttt atgtccttgt tcaccatgat gagcgatcac ttttgctaca ctttctcgat
     2701 cattcactaa tattgtatca atacctgctg gtccttctgg acaattcaat gcgataactg
     2761 gaatactcat ttttgtgagc atttcaaatc cagaccactc ttcttcggat aagaagacaa
     2821 tcagcccatc gatcacatgc ttcatcaggt attcaatttt ttgttcaatt ttttctggct
     2881 gatcggcaaa cccgcttaat aataagctat acccattttc ttcagctact ctttcgatcc
     2941 ctgagacaac ttccgaccca aaacggctgg aaatcccgtt tatcaaaagt ccaatagaga
     3001 agcttcgatt attcttcaac cctttagcaa tgatgttttc tttatagtcc aacgctgaaa
     3061 tcgcttctgc tatttttttc atgttctttt ccttcagttg ctgaccattc aagtaacggg
     3121 aaactgttcc cactgaaaca ccagccatct tagccacatc acggatcgat gccatacatt
     3181 ctcactcctg ccagttacat ttgatttctc tttcttgttg ttcgattatg atgaccgcat
     3241 tctttgcata gtgttgcttg tcatcaatat ccatataact aaagtcttca gtagagcaac
     3301 ttcctctcac tattaattat acttaaacaa gtatttgaac cggttcattt ttaatatagc
     3361 atttttttaa aagattgcaa cactttttta aaacgctttc tgaaagtggc ggattttttg
     3421 cctttttaaa attttattct aaaatttact ctatttgtgg gtgttttgtg cgtttttctc
     3481 ttccatccgt ttgaagtttt acattccaat ggtataattt ctttatccta tcaaatgggg
     3541 gaagtatttt gaagaaatta atcgtactat ttattttgtc tgcgggactt ttgacattga
     3601 ctggctgtga tttgtttggc ggctctgacc aatcctcgga cgaaacaacc gattcttctg
     3661 tcttaaaagc ggaaaaagca gataccacca acagtgtata tacgctaaaa gtacgtaaag
     3721 atatggattt atccgcggga cagttgacat ttacagataa aaaaatggaa tggacacgca
     3781 cgtatcagac acaaggaact gattcttctg aatcatctga agaaaaaaca gtgctgacag
     3841 atatcaagat cactaccaaa gatgacgaat atattatttc aggaaaagaa aataaaaaag
     3901 aaacaacagt catatttaca aaaatcggta attaccgttt gaaggatgag gatggcaaca
     3961 tctacagtct atagtgccgt ctgttggatc ttcttttgtt tctccccttc ttttctgcta
     4021 tagtaaagaa gcaagatgat tgtaaaggag acgaaatgga tgatcgatag tcctgattat
     4081 tgtacatgta ataacaacaa aaatatccat acagaattta atgaccaagg aagcttgagc
     4141 atatgcgaca actgtcacaa actagtcaaa gattcattta aaacatttga atcttatcct
     4201 gttcaaaatt atgtaaaaag tagaagaaga taaacaaatc caagcaaaac tcactatggc
     4261 tgatttttat gaacttcaaa ctcagcttct tcctttgatc aagcttcatc agtaaaacaa
     4321 acagtggatc atttcagttt acaaatcaaa gagtaaagat ttatttattt caaatatttg
     4381 aatataaaat aaccaaacga tagaaaggct tattctacat cacgtagtaa tttttctttc
     4441 ccgtcgtttg gttttttatt tttagcctaa ttcttgacct tttgcatgct ggttatccgt
     4501 aaaatatttt ccagcaggct ttccttccaa ttcagctaat ttttcttgga tctgacggat
     4561 cacctttttt gcatcttctg gattttctac aaaatcgaat ttatccccat ccacttgtat
     4621 tttaggacat acatcaaaat cttcatacca ttgattatag cgggtgttca acgtttcata
     4681 ataggtatat aacgtctcat cgctttctag ctgttcatag tcacgcccac gttttttgat
     4741 acgttctagc atcgtgtcca aagaaacttt gatatggacc atcaaatcag gccgtttttt
     4801 tggagcaaca tcatctaact cgtttagcat cgtatctaat aagctatcgt actgctgtac
     4861 ttcgatatcc gttacccgac ctaaatcagc gtttagatgg aacaacaggc tatcttcata
     4921 gatggatcgg tctagcacat tgtcatcgtt cactaaagca tttttcattg ctaaaaatcg
     4981 cttgttcaaa aagaagattt gcaataaaaa agcgtactgc tcaggatttg aataaaagag
     5041 cggcaatact tcgttatctt cgacattttc ataaaatggt ttagaatcaa tctcttttgc
     5101 aagcatatcc gtcaaactgc tcttacctgc accaatcgtt cctgaaatca aaatcactga
     5161 catacaaccc ttcctcttct ataagtactc gcctacgtaa gtttcaacag gcaatgtttc
     5221 aaaatcatat tcacgtacag cttggtctga ttttaaatac gtatggagac tttgtgtaat
     5281 catcaagttc gtaggtaccg tctcttcttg tacaatgatc atcggttttt tcaacatagt
     5341 tgccacacct aattcgtaag ccgttcctgg atcgattgtt tgatgctcaa agtcaaggat
     5401 agcaacaaca aattctgcat ttgtcagttc ttccatatct ttttcgtata cttcctgcgc
     5461 ccatccagcg ctgaatagtt cataattgct ttcttggtga tgacgtggag agtagaaact
     5521 tgtgactgtt ttgttttcct ccaacgcttt ttcgatacga cttactcgat cgatttgctc
     5581 ctctgaaaaa aatggtcctg ctaaatatat ttgactcatt attttgcctc cttcaataat
     5641 aactgatttt taatagatac tgctcgtaat tcattgacca gttccaatgg attgttggaa
     5701 aagattactt gatccaacca ttttttatcc ataaaatcct cttttgcaaa agtttctaat
     5761 aattgttgca agccgctgta ataaccgcca atattcaata ttccgattgg cttgtcgggc
     5821 gtcagaccga ttgctatcca agaaagaagt tgtgagaatt cctccaatgt acctagacct
     5881 ccagggaaaa cgaggacagc atctcccttt tcaaataata atacctttct ttcatcgatc
     5941 gtttctgtag gaataaatgt agtaacatct tttccaggca attcagtttc aaaaagccca
     6001 ttcggataga tacctgtgac ttttccgtta ttttccagta ctgttttact aatcactccc
     6061 ataatcccgg agtatgaacc accgtaaacc agttcgattt cttcttgtgc catgtatttt
     6121 cctaacgttt gcgcaataaa tttgtaactt tctttattac caaaacggga accgcaaaaa
     6181 acagtaagtt tcataaaaaa acactcccta actcaaaagt ctatcttcat tctacacgtg
     6241 tttttttaaa ataaaagact agattttgta gttttttagt ggtatatagg atagacgcca
     6301 ctatatatag tatttagaaa aaaatcattt ttcagccatt ttatagataa aacaaaaata
     6361 aataacgtgt tttcatcgat atgaaatgac ttacttatag aagatactgg gatttcgtcc
     6421 tatatgccaa taacgtgtat tattttaaac aacaaatact tgtttctttt attaacgaaa
     6481 aaatcatttt gttctcgtgt tatgtttcac tgataaatga tgtttctcta aataaaaaag
     6541 ccgacgaact aaacgatcaa aatttgatca tttttgttca tcagcttctc ctaaaaatct
     6601 ttttgttaac gataaatagc tggatttgta aaaatatcac ttgaatcgtc actgctggaa
     6661 gaaaattcag tgataaccgc acctgttgcg cctgcctgga actagtgttt gatattgggc
     6721 tcgaacgtgt actgatctct agcatgaaga ataatttctt tttttactgt ataataactt
     6781 tctttccttt ttggaatagt acaggaaagt ttttttgttt cctttccttc gacataaggt
     6841 ataccagacc ctttcgacaa cgaaaggttc tcatttttcc ttcatcgtca tttcaaggag
     6901 ggtgccgatg ctctggacag tttcgatcgc tcctaaacca aaatctgtat attcgattct
     6961 ttctaactct gattctttca taccattcct gtctcaaggt acatctattg tacttttttc
     7021 ttgtaatcca ttctgcctct cctaactaat tgtcaaatca tcccaagata tgtcttcttc
     7081 ctctttttct tcttggatga tttctgcatt cggatctact ggttttttca ataataaaat
     7141 ggttattcct gtgacaaaag aaccagctaa caatgcacca atgaatgccc atggtctggt
     7201 catcgtagcg actgcaaaca ttcccgatgc cggaatacgg ctatctgcgc cccaaaacat
     7261 gcttaatccg ccacctactg tcccaccgac tcccgttgct atgacattgc gtgccaagtc
     7321 gttcaatacg atagggaaac atccctctgt gatctgacag atccccatag gaaaagcggt
     7381 cttcaatgta tcgatttctt gacgattgag tatattttta cgaatgattt tccctaagaa
     7441 ataagcgatc gtcaagccga atggggttac catcgaagct aaaatcaata cagtgattgg
     7501 ttcatagatt ccttctgaaa atcgtgccac acccccttat ccagaataaa tggtaatcca
     7561 gaaacagccg tgtatctgcg gtaataagcc taaccaaacg aaaaatatga gaagctattt
     7621 ttgtttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc tttttttatt
     7681 tatgtttcat aatcaatatg gtaaggatgc agctccgtat ggtagaccgc ctccgaatat
     7741 tcaatcattt ccccaatttg cgtagacgat ttccgtatcc gtttgatgcc gatctgtgcg
     7801 tctgttttca gaattccttg ttcttcttct gttaaaaata ctgccgagaa atcatcagaa
     7861 aatgagtcaa tagtatatcc ttttttcgtc agcaaacgat acaaagattc tttgccaaat
     7921 tcttctctgc tgaattgcat catttcttgt ggaagataat gaacaaaata aatataaggc
     7981 tgattgttca aataatataa gcgcttaaaa caaacagctt gctcaccgaa aaaggaaaac
     8041 aaaggatctt ccttctttat atggaccaat tgcaaatcca gcgtttcttt ccggacttca
     8101 tatcgggagt tctccaagat ttgcgtaaag cttgccgctt tggataatcg attgtaagga
     8161 cgattgctta gaaccgtcgt cccacgacca cttttctttg cgacatattc atctgatgcc
     8221 agcagttcga ttgcctttcg gacagtgatt ttgctgactt gaaacagttc ttctaattca
     8281 ctttctgtcg gcaacatcga accgactgga tattttcctg agaaaatatc ttctttaatt
     8341 ttatctgcga cttccaagta tagtacacgt gtccttttca taacgtcacc ctttgcttta
     8401 ctattactaa aagaatagca cataaaaaat aaaaaaagaa cacaaatcac atgtgagaag
     8461 tgtgttcttt ttcattcagc tgctttaatt acaagtttac gccatttgag gcaattactt
     8521 ctttatacca atcaaaagat tttttctttg atcgattcag tgtcccattt ccttcatcgt
     8581 ctagatccac atagataaag ccgtaacgtt tactcatttc tcctgttgat gcactgacaa
     8641 gatcgataca tccccaaggc gtatagccca tcaaatctac accgtctgct actgcattag
     8701 ccatcgcttc gatatgacga cgtaaataat cgatccgata atcatcaata atcgtgccat
     8761 cctcttccat atgatcaatg gctccaagac cgttttcaac aatgaataat ggtttctggt
     8821 aacgattgta taattcattc aatgcgattc tcagcccttc tggatcgatc tgccagcccc
     8881 attcacttgc ctcaagaaac ggatttttta ctcctcccag caagttccct tctgaagttg
     8941 ctgcttgtgg atcagtctca ttgattgccg tagacatata ataactaaat ccgatgtagt
     9001 ccactggata ttcttttagc aactggaagt ctttttccgt ttgctctaat ttttccggat
     9061 cgactccata ttttttatgg gtacgcgctg tgtatgcagg gtattctcca cgtacttgga
     9121 catccgtgca gaaaaaattg aattcttggt tttggatcat tgttgctgcc tgattgactg
     9181 gattggcatc gatgctgtaa gttgttgcat agataatcat gcagccgatt tggatatccg
     9241 aacgcaattc gtggccgatt tttacggctt tgctacttgc aacgaattga ttatgccatg
     9301 cttggaaaat attttggtac tctcctgctc cattgctttt tactaatcct tgacttaaag
     9361 ctggaaagtg aaaggccgaa ttgatttcat tgaaagtcat ccaatatttg actttactgc
     9421 tgtatctctc taatactgtc tgagcaaatc gttcatagaa gtcgatcagt tttcgatttt
     9481 tccagccacc gtattctttt gccaaatgaa gaggcatttc gtaatgagaa atcgtgatca
     9541 caggttcaat atcatatttt aagcactcat caatcagctg atcataaaat tctaatcccg
     9601 cttcgttagg agtaccttcg tctccatttg gaaaaattcg tgtccaggca atcgaaaaac
     9661 gataacattt gaatcccatt tccgcaaaca aggcaagatc ttctttgaaa cgatcgtaat
     9721 gatcgattcc tcgatgattc gggtaaatat atttttcttg atcaatcgtc cagtcaaatt
     9781 cttcactgcc gataatcgca aagcgctgct tacctccagg catagcatct gcaacggata
     9841 gtccttttcc gtctcgatca taagcccctt ctaattgatt tggcagcagt tgctccaccc
     9901 cataaaaatt tttctgaaaa acgtgattgc atcctgattt cctccttatt ttacaattgc
     9961 cagaacatct tcgtttgcaa taatctcttt ttggtctaat tcaagtatat cggcaaatgc
    10021 atcagtattc gtaatgacaa ctggagtgat tacgctatag ccagctgctc taattgcttc
    10081 gatgtcaaaa cgaatcaata aatctcctgc tttgacgaga tccccttgtt tgactaagat
    10141 ctcaaagcca ttcccattta attctactgt atccatcccg atatgaatca aaatctccgc
    10201 tccgtcagtc gttgtgatcc ctacagcatg tccagttgga aaaatagtcg tgatctctcc
    10261 gtctgcaggt gcaactagtt ctcctacttc tggatcaata gcgatacctt ttcccatcac
    10321 gccagaagaa aaaacttggt ctggtacttc actcaatggc agtactttac ccgttaaagg
    10381 actttttaag gtatgccgtg ctgaagtaat cggttctcct gcattcgcat gtttgttttc
    10441 taattgattg tcttgtgttt gttcgtcaaa tcctaaaatc aatgtaccta cgaatgcaag
    10501 aacaaaagca attcctgtag ctattactgc tactgtcaca tttgactcta caccatccac
    10561 cgtattaatg aaagtcggaa tcgtcaataa gctgaccaaa ctgctggaga aagcttttac
    10621 tccggaaaat ccgataatag ctccaccaat acctccagaa atacaagcgg caatgaatgg
    10681 tttcttcaaa ggtaatgtca caccatacac agttggttca gtgattccaa ataacgaagt
    10741 cactgtagaa gaaatcccta atgctcgaag tttcgtatct tttgttcgca atgctacagc
    10801 taaagcagcc ccaccttgtg ccaagatggc tggaagaagc attggcatca atacatcaaa
    10861 cccatattgt tcgatattca aaaacatgat cggtacgaat ccccgatgca tgccgaacat
    10921 cacgaatact tgccataaac ttcccatgat tagacctgct acgattggac taaatccata
    10981 aatcgagttg aagagaccgc ctaaagcatt accggcaacc gtcccgattg gtcctaaagc
    11041 aagaaatgtt aatggaacca tcactactaa aacgacaagc ggaactaaaa tcatttgcag
    11101 aaattgcgga atcacttttt ttacgaatgg ctctaattta ctttgaaccc atacggctag
    11161 aatgattggt atgacagaag aagtataacc agatggtgaa agaatcactg ggatcccaaa
    11221 gaaactgata gcttctcccg caccagcaac tgctgtgata gctggataca caagtgccat
    11281 agcaattgct actgctaaaa actggttggc tttgaacttt ttagctgctg taaaagcaag
    11341 catgacaggt aagaaggtaa agacaccatc tgcttcagca aacaatattt gataagtacc
    11401 gctatcagct gtcagccagc ccatcaccgt tgccaatgat aagaatcctt tcaatacccc
    11461 ggcggctgcc atcgccccta gaaaaggtgt aaatatacta gagataatat cgatcagacg
    11521 attcaaaata tttttggact cttcttttcc atcctccgaa actgcaagac cttcctcatc
    11581 cacgatcgat tgataaacat ctgctacatt actgccaatc actacttgat actgaccgcc
    11641 actttgtacg acttggatca cgtccgggtc ttcttttaac ttttgcgtat ctgctaaaga
    11701 ttcatctttt aatctgaatc gcaaccgcgt agcacaatgt actaaacttt tgacattctt
    11761 ttgtcctcct accgcttccc atacacgacg accaactgct tgattatcca ttgttttttc
    11821 cttctttctt ttttatttaa aaaataaaaa cccagattga aaaaaacagt cacaaacatt
    11881 cgtgaaccta ttttttcaat ccaggttttg cctgccatac agtaacaatc cttatgattg
    11941 gatgcttttt ttctcactta ctactctttg aatatgaatc gttaaataaa tctgctctgc
    12001 gatccccatt tcctgttttt gcgtttgtcg aagatactca ttgatccgtt cgacacaatg
    12061 aaacgctttt ggatactttc cttgaactaa tgcgtagaga aattcatctt gctcgtcaga
    12121 tccttcccct ttgagataac gctgggcaaa aaactgcaga tgagtaatga tacgctgata
    12181 attcaaagat tcttcatcga acacgatgcc gaagaattta ctgatgatac tcagtatatc
    12241 tcttaccatt gtggtagcgg tcatcgtcac ttgcatgtct tcgtttcctt gttggctatt
    12301 cacaaaatga aaggcaataa aaccggcttc atcctcagaa agttccacac ccgtcttttg
    12361 tttcacgaac aaaagagcct ctttcgctgc ttgatattct ttcggataaa atttttttgt
    12421 ttcaaataat aatggattag gcagttggat attttctttt gctcgtgtga tcgcataatg
    12481 aagatgatcg gtcaatgtca gataaatatt aggcgaaaat tcaactttta gtttatcttc
    12541 agccagttcg actacttgtt tgacccattc gatctcctct gccggaatat cagccatcag
    12601 ctgctggaat tggtttttag caaaagaatc tcttaaaaca aattcttttt ggaccgcttc
    12661 tggcgaaatc tcgtccccaa tctttttttg aaaagctagt ccttttccca tacaaacgac
    12721 ttcttccccg aatccatttt tcgaaatgac gacattgtta ttcaaaattt tatcaatttt
    12781 cattttgctt cgccctctca agaaaaataa aaaaacctag aattcgccta ctgtttctta
    12841 gctgaattct ggttttgcct gccgtacagt aacaatccat ttcttattca aaatcatcat
    12901 accatcgaat ttttatttat gcaagccttt tcatataaaa aataaaaaaa ctggaacaca
    12961 agtctatcta gaaaacaacc aaatcagaga tgcacctctt tgcgtttctc tttttttcag
    13021 caaattggtt tttccttatc gattcatatc ctttggctgt ttttaatctg taacgactta
    13081 tgtcccagta tcctaaaatg tttttgtctc tagattgaaa gcagatctca tatcatctgt
    13141 cgcatctttt atcgatgtaa cactttccag ttctccttgt acaactagtc gcgtgacggc
    13201 gtccatgtcg ccacaagtaa ttagtgtgac aatttctttt ccctcgactt cattcagtaa
    13261 ttgggtatct gtaggagcaa ctttttcttt aaaaaatgtt ttgtatgtat acacattttc
    13321 taaatctgtc aaatagatct tggcacccat ctctaaattt tctagcggtg taaacagcaa
    13381 ttcagggttc gttgcacgat ggcttgctaa ggcataattt ccttttccca tttcttgatc
    13441 tggagataat gttcctgctc cgtaaagtag tgacgtatta tccaagcctt tgaaaaccgg
    13501 taaattgatt gaaactgaag ggactgcgat actagcaatc actggtagat ctgttcctct
    13561 catttgcgac ctcatgaccc cttctgtcgt cattggttct accgcatcaa agtcaaaact
    13621 gacatcttta tcgttattct ttttcagatc ttcttttgtt acattcgcta tagcatattt
    13681 atctcctgtc tctctgacaa aataatcctt gatctgttca ttgaaaatca aagctagtcc
    13741 tactaataac aagaggaaca agaggatatt gactagccat ctttttgctt ttcctttttt
    13801 tttcattttc tctccctcca gactcatttt actgtttttc ttttgctctt tccaacagcc
    13861 gaatgacaca tttctacaac tctgtgtatt tttttcgtac acctgcgctt ttttctacag
    13921 gatatttcaa attatttttg atcaattttt cttcaatgat ctcatctaga tccaggtgaa
    13981 ggttgtctgc catcatgtat gaatagatca atacatctgc cagttcttct ttgattcgtt
    14041 ctaattgtgt ttcttttact tcttccggcg ttttccattg aaaaagttct aacaattcag
    14101 atgcttccaa agaaatagag atagccaaat ctttttcgtt atgaaattgt cgccaattac
    14161 gttcatctct aaactggttg actttttcca tactattcat ttgcttgcct tcttccaatc
    14221 acttcttact tgattaatca taccattttt ttacataaaa aatagatgat gcttttaaaa
    14281 aaactcctgt tttcttttat ttaaaataaa tgtcatagaa aatcaataat ttaggtagcc
    14341 accatgtgta tgcagtagcg tgtcttcttc ccttgaggct ttttgttttt cgatacgttc
    14401 ttcttcccaa acagacaacg ctactagaat ctctcttaat gattttcctt cttcggtgag
    14461 cgagtactct actttaggcg ggacttggtt ataggctttt cgatggatac tttcatcttg
    14521 ttctaactct cttaactgct gggtcagcat tttttttgtg atcgtaggga tttgcttgct
    14581 tctccacgcc taaaataagc gatattgctc ttccctttat ctggtgtaag caaagcagtt
    14641 agcattttga cgatcgtggg ttccgctttt cacttcttat ctacttagta tcatgaagta
    14701 tactttcgta ctaatactaa gtagcggaag tgtcaactac tttgtttaac tacatagcaa
    14761 aagaaacaga agggtgacaa cggtcatcac gcttctgttt cttttgaata tgctgtgttg
    14821 ttgtctctat ctccgtgtag actttcctca aacttatcct ttgacagtaa gtcagtaggc
    14881 tgatttgctc tttctagatg ccagatacat ttctacaaaa aaatagtgat tctgttttga
    14941 aaattcgatt tttagttctg cattttcgta aacatccaac atttttttcg cactgtatag
    15001 acctaacccg ccattttttt tagaggtttt tcctcttttg accatctccg atagcgtcaa
    15061 attcgttgga tttgccttgt tcgctatact aaaacgtgat ccctcttttt cttccagcag
    15121 attgatcgta atcggactct gatcggctac acgatgatcg aatgcgttag aaataaaaat
    15181 agataaacag cgaataaaaa caaccagatc aatcgggatt gttttcactt cgacaggaat
    15241 aactacttgg atcgggattt tctccgattc tgctttttct agaaatttta aaaagacacc
    15301 cctgacagct ggggatacaa tatttgttag ctgttgtatt tgcggttttt caaaaatagc
    15361 tgcagaatga tcttctaata tttctaaaaa ttccaacgtt tcttttttat tttcctgaag
    15421 agtaattttt aatgaaaaca gtagactttt ataatcatgt ttgaattcct ctagatgatg
    15481 gtaatattct tcatttattt gatacagttg aagcgattcc tgatgttgtc tttcttttac
    15541 ttggaaactg atcaacataa tggaaaaaaa caaagaaaaa aaactgatga ctatcgacaa
    15601 aagagataga agcagataat tagatgagcg gaagtgaaca cttcccaata ccaagtaaat
    15661 cacgccccag cttaataaaa tacaaatacc tccaaaatag tgttcagttg gataagtaaa
    15721 aagcaagttg agaaatctcg tctgtttgtc tatacttttt actaatagat agaaaataaa
    15781 tgcgataagt agtgcaaaaa aacttgctgt tccaagatgt ctctttccta aataatccat
    15841 cagatcaaaa accagaaaaa agctgagtcc agtgatagca aaaaaaagat tactgatcaa
    15901 acagctgata aacaggtttt tcgtagtaaa ataaaaaaga aacatcaata ataagccaac
    15961 taggtaagga aagctcactt tagggatagg aagtaaaagt gtcaccacca tcaaagctat
    16021 acaaccaaag ctgtttttta ctagcagttg tttcgaaaag aataaataga tacctaaatc
    16081 taaaatcaaa gtaaaggtca aataaagtgt cactgttgcg accatcttat tcctccattg
    16141 ttttgtttca aaatgtacac atattttttt aataggagcc tgcatatgaa aaagtatttt
    16201 atcatagaag ataaccagtt tcacagcgaa tggatcatca gagaattggt caatcatcat
    16261 atcgagaata tcgtgcagct cacttctatc tctcaaatca atcagttgat ccagttgggc
    16321 actattgata atcacgatgt tttctttatc gatatcgact tgaagcaatc actcacaggt
    16381 attgatgtcg ccgaacaaat acgtaaaaaa aatgcacata gtaatattgt ttttttcacc
    16441 gcttatacga attttgcagt agagatcatc aatcggaaaa tccttccgat tggatatatc
    16501 agaaaagaca ggaatttatc tgaacaaatt cgtcaagtgc tagaacagat caattggaaa
    16561 gaacagcaga tcgtaaatgt cgataaaatc tttattgatc aaaaaggtgg aagtctcgtc
    16621 ctgtatcctc gggaaatttt atatattgaa agtatcaaag gtttgaaaaa ccaagttatg
    16681 gtcaaaacaa ctacagaaga taaattagtg aatacttctt taaaacaatt gaagacagag
    16741 ttagaaaagt atgcttactt tttactttta aaatcttact gtatcaactt atcgcaattg
    16801 cagaaaattg attctgctca ttatgtgtta gaatttttca acggtgactc cttactcgtt
    16861 ggtcgaaaga tctttgaaaa gacaaaggaa cgtttccatg acttccaaaa aactgcttcg
    16921 agttgatgaa taagagctga gacgtaagca tagcagattt cagggagtgg gccttgaatc
    16981 ggctccactc cctgaaatcg ttgatatgat tttacgcttc ctcttctgta ggagcatctg
    17041 ttaacgacca tgtgaatgtt gaaacgtagt tgtctacagc ataattggat acaggaacaa
    17101 ataattccac tgattgtctc ccttgttcca cattctctcc taatgaataa atccatgtgc
    17161 caatcccttc atttgctcct gcagttacta gatctactgc ctgatctgct gggatttcga
    17221 ttccttcagc gccagttcca gaaacggtag atggcattcc actgctatta gttgcgctat
    17281 ttcgaatcac ggcgtttttg aatttcacct ttgaaccagt tagtttattc gtatcagatg
    17341 ttgtaccaat aaattctgtt cgtttgactg ataatttcca accggcataa tttccgcttt
    17401 tatccgttac ttgtacgaaa ttctgtactt gtgtttgtgc tccagtactg tccgttccgc
    17461 tttgtaatgc agcatatact gtttttgctg tacctaactt gattgttcca aaatcaagat
    17521 ctggagcttg gtcaatcgtc agtaatccgg atgttccctg atttggttcg tcaggtgtaa
    17581 aaggagtatc aggatcattt ggatcaacag gaggaactgg aacaacgtct tcaggatttg
    17641 cagcatctgg tttggtcaca gaaatcgttc cttctgtagt ggctgagctc ttattctcag
    17701 caaaaacact ttgtgtacat aataatagac ttccagctag aacggtactt aatgcaattg
    17761 tttttttcat ttctttttcc ttctttcttt taaaataaat aatccagcaa ataagccaat
    17821 aagaatgact ccaacaagta tcagccatcg ccagtcaaat gtttctggct gattttttaa
    17881 atcaatatcc ttttcattca attggtccgc tgcatcatca gttatttcaa aatcggtatg
    17941 gagattccag cgtttttctt tccaagcacc cttcacaacc gcttgataat gtcccggagc
    18001 taattttttt ccttctaatg ggacaagtaa atcaaaattt gtattgggtg ctacatctat
    18061 tttttctttt tttattgaaa ggtaaggctc ttttgtttct tttttgtaga tttcaaccga
    18121 tgtagtcaag ccgaacatat aagaagcatt tggattttga agattcgcct taatcacatt
    18181 tcttccatcg ctttgatccg cataagcttt ttttaatttc aaatctgggg aagactcttt
    18241 ttcgctattt caaatcaaga ttgctttcac ttgagaaaac tgattgacga caccaactgt
    18301 acttttgtta gaaacgttct cttttttttc ttgaaacgtg atccctcctg cgaccacacc
    18361 agccagctct ttttctggcg ctttcagtga aagtgtctca gtgaccgcac tttgaggagg
    18421 aacagttatt tctcccactg tttttgctaa gtttttcata tcataactta atgtttcatc
    18481 aaatttactt tttgattctc catattcgat cacaccgttt gaattcgtcg tagcggagtg
    18541 gatcaatgtc tcaactgtta ctgatttatc tgttcggtta aagaccgtgt aggggatttc
    18601 ctgtgttttc ccaggctcca tccttacatt tatatatgtt ttgttcttat caatctgatt
    18661 agccggtaat tcaaaatcta ccgcaaaatt gaattcggag gcatggacag gtgtctgcca
    18721 aaggatgaat ccaaggagaa ggatcataag taaagagcag ttgtttcgat gacttttatt
    18781 ccatattcga ttcatcaatc atcctccttg atctttattc tgtcggcgca tctgctaatg
    18841 tccatgttat ctctgctgag taattaccag ctatctgatt ttctttggtt aagacaagtt
    18901 taaacgcccg accgttattt aaatattctg aataatctgc ttgcacattc gttgaatttt
    18961 gtcctctgac aatttccagt tcttcttcat tcactaattg atactcctct cctttcttga
    19021 aatataggac atcctgcata gaaaatcctg catcatttgt gaaaggctga gataatttta
    19081 tcgttaagat ccacggattt ttcgtttctc gctgatcata aatccataat ttttcctgta
    19141 tatccatttg gccaaatgct tcttgtgtca aaccagttgc tttgatctga ccaaaatcga
    19201 gtttggaagg ggcaagaaat gagagaccac cttctactac gatcttcgta gcaggtgcaa
    19261 aaggcggggc atcatgataa acaactggcg tatcacttgg attgtgattc ccaatttcat
    19321 cgttggtact tggcttgttc tgcacaccct tagcagtagc ttctcctgct tggtcgttta
    19381 atacaatttc taatacatct cctgcaaaaa gttgattttc tgggatagaa agaacaaatt
    19441 cccctgtatc atcaatagtt gtttctgaca aaacagcttg attttttttc aatgaaacca
    19501 aggctcctgc ttcacctgta cctgtgatcg tgcgagaagc tgcattgatc aatgtggtac
    19561 ttaaattagc aggttcagga ggaatcacat cgactgtcgt tttggcagag tccagttctg
    19621 tcacttcttg gccacctctt acttttttag cagcaacaac ttgcacttga tcctccgttt
    19681 ttaatctctc atcattgggt acttgaactt cgaacaagcc gccttgttcg ccttctcccc
    19741 atgggttata tcctgcccct tcattttcat ctgtttgtcc ttgcgtgaga gcagtaattt
    19801 ctacagatga tttcgtcgta tctgcgtaag taatacgtaa agtcacttct acttcgcctg
    19861 tccaagcatc tcttggcttt gcctcgacac cttcgggaac aacgacatgc ccatagattt
    19921 ttttatctgc attcgttggc actcttaagg attcaatcat cgggagctga ttattggcac
    19981 tgattcgatt ataatttttc attccggcac ctgtctgagc aaattcattt gttaaacctg
    20041 cactagggct tgatacgaaa ttgcctaagt cggtcccatt gatcgataag tctgtcatat
    20101 cagaaaaata atccgggtct ttttcaaagt tatcgaatgt tgcgcctgtt tttcgccata
    20161 aagcgatttc tgaatgggcg atctttaaag aagaatctcc atttccttgg aatacccagc
    20221 cgcctatgcc attggtctcg cttccaatcc gtttgttttg gaaatcaaaa taggtaggag
    20281 attctaagtc cacattcaac acatccccac tgatgatgcc ggtcttatct tcccctgttt
    20341 tcccgaccat gactaaataa gttttttcac ctgccgtaaa atttaaatca atatctccag
    20401 ttgtatctac agttgctccg gaatctgcgc gtatatcgat atttgatttt tctccagtga
    20461 ctgtaaaatc agctgaacct ggactttcta gaaaaccaga agcgtcataa aattggatcg
    20521 cttgatttcc ttgattgagt ctgttcacgt aagatttgcc atcacctgta tttcttaccc
    20581 tcaatgagcc tccattggtt actgtcagct tttgcgtacc attctcaaaa cgaactgcgc
    20641 tggaacgccc actctgctta tcgatgtcga taactgatcc ggctccttct acgatcaaag
    20701 aaaggccttt tcctacaaaa cgcatcccat tgttcccggg ttgatcgtca tctccagcca
    20761 ttttgaccga gatttgtcca ccacttgata catggatttg actagaatct ccttgcaagc
    20821 gaaatccgcc cgtagttgct gtattgatgg tgattttccc accattcgat acagtgactt
    20881 tcgatcctga acctctcatt tggatgattc cgcgattgtt gtcattggta atattgatct
    20941 ttgtatttac cgaaaattcg cttcccgaac cactgacttc aaacatgaca ttaccaggta
    21001 gcgtatcgac tgcctctcct tcatcgctaa cgccatctgc ccgatcataa tcaagagaga
    21061 cctttgaacc gcttaagata tggattcctg gcgctgtttt tccactacca gtctcatctc
    21121 ctgaatctag aaaaccgtag aatttcccta gatacggtcc ataaagttga ctaccattga
    21181 tcacattcaa ttgatagctt ctacaaaaat aggctttacc cggagcggca ttcagtcctt
    21241 ccttcactgg ttggacatcc aacacaccgg aaacaccatc taatgtgaca gttgcacgag
    21301 gagcgtaaat cagtcctaat ttggacccat catacaaatc aagtgtcccg gtaaatttta
    21361 tttctcccgt aaaatgatat ctgcttcccc aaaaatcggt catataagca atgataatgg
    21421 catttccatc gatatttggt gtcaatccac ctggatgtgc agctgttcct ttaaaattct
    21481 ggaactcgat cgactgaggt atctcattga tataaatatg gtgtgtgccg aaattgtgag
    21541 cgaatccacc aaaatcaata acgatatctc gtttgatctc taattccctg tctaaagtca
    21601 aaggctgaat cagtcggatc ttagttactg attcgtctgc gagaagctgc gccagttctt
    21661 ctgttgtggc agcttcgctg tacccatccg ctgaaacagc tcttgccagt gccgcttcac
    21721 tgctatcaga cgtttgttca gctacttctg aggtctcact tgtcttattt tcttcttgcc
    21781 ttttattagt agaagcctca ctttgagatt ctgtagtttc agaagtaatc gattcttctg
    21841 aaaaagtagt cttgtcaaaa ctttgattct ctgattgcgt tgattcgttt gttttctcta
    21901 catcggcaaa aacggatgtc accatcaaga gagggaagac aaacaaaatc gacgcaatca
    21961 gcttttttac acattgattc attctccacc atcctctctg acgttttgac aattttattt
    22021 tatcgtttta atctatttcc aattgtactt tcatatttaa aatatgtatt tttatggaaa
    22081 aatttttcta ttaggtgtaa atttcttttt tgtacaaaaa aaaggctgtg acaaaagtct
    22141 tgtcacagtc ccttatcccg aacaagcggt gttctcccgc caactcctcg gtcacaacct
    22201 ccattttaac aataaacctg taaatgtgag ggcaatactt tgattgtcac aggtaattta
    22261 tccccttcat ctccatcaac attcgtcgtc aattcttcct catcagctaa tgacacaact
    22321 aattgctcac aagtaaggta ggctagattt ttcgtagatt catctactcc ttttaatagg
    22381 tctggcaatg ttttaagcgt atcgatcaaa gaagaatctt ttaaataaac caaatggagc
    22441 ttaccatcat ccacttttgc atttggaagt aatgtttcaa aaccgccgac tgagttagtt
    22501 agtcctatca gcaaggtact gctctcaatt tcttccttct ttccatcaac ttctacgtga
    22561 aacgagtagg attgtgtgga agctaactgt ttgattcctg acatgaaata agctagtttt
    22621 ccaaatttcg tcttcttctc cgcatctaca tcattgattg attcagggat agagccaatt
    22681 gcgacgacat tcataaaata gtcgtcattt atttttccaa tatccaatgg cttgactgat
    22741 tcaatcgaaa aatgattgat cgcttcctca ggttctagcg gtatccctaa tgctcgagcc
    22801 aagtcattca ccgtacccaa tgggaaaaat ccgaaatttg gccgatgttc ttgttcagcg
    22861 attccactga ttccttcgtt gactgttcca tctccgccca tgacaaacac actatgatac
    22921 ccttccgtag ccgcttcgcg agtaaagttt tttgcatccc ctgctttttt tgtatgcaag
    22981 acgaccactt catcaaacac cgattccaat tttgctatcg ctaattgctc aaattccttt
    23041 gcttgttcac cgcctgaact ggggtttacc actaataaaa cttttttcat cactttcttt
    23101 cccttctgat tgatttcttt aatataacga tagcataaag aaaaaaatta ggctgtgatg
    23161 aagtactgtt catgaaacat tcatttattt tttgacaaga taaaacatcc atactatgaa
    23221 actgaggaaa attaaaacaa tgatatagga aataaaagaa aatgataggc attgtatacc
    23281 taataaagta aaaatcttag gtggcagata aaaacacaaa aatagcaaga gaatgtaata
    23341 gcgtttcttc cactttttgt tatcagacct tatagcgatc agaggaatac caaatgcata
    23401 actgaaaaac tcttgatgta ctgttaatat gtcacggcct tgaataataa atgcgacaat
    23461 ccccgctata attagtgtgc ctaacaccaa aacggaaaat tgatgaatat attttatata
    23521 caaatgatcc attgtacttc ctgccttttt atttgtgaga aaaacaaatc cgtatacgtg
    23581 aataacttct agaaataaaa gaaatttgca aaaccactcc atttatctac ttctacgtgc
    23641 attttttgat attgttttct ttatttaaga ttatacagaa aaatatctag ggtcgctgga
    23701 ttctaagtaa aaaaaatttt taaaataaaa agaactaaat caagtagtaa ttaaactact
    23761 gttgatttag ttctaataaa aagatttaga tgaacagttt tattgattta aatagttcat
    23821 attttttatt tataccgctt atttactact tgctgtacta gaatctgaag tgaatgtact
    23881 caaaacattt tcaaagtctt tgtctttgat ttttacatta gcgtctttca atacttcgcc
    23941 aattacttta ttctggaagg tactgtcact taattttgtg tctgttgcaa tttctttcaa
    24001 ttcttttttg tatttatcca tgtcgttgcc tttgttttgg tttttcacca ttttaaccac
    24061 ataatattcc gtagtataag ttgaagcatt ggtagaagtg atcacatcag aaacttgacc
    24121 atctttcaac ttaaaggctg cttctttgac ttctgccgga accgttgtag tagtggaatc
    24181 aaatttcact tttccgccat cttcttttgt cgtagtatca gttgacttgt ctttggctaa
    24241 tttagagaaa tcgtctccct tatcagcagc tttcttcaca tcttttgcat catcttcact
    24301 tgctacttgg ataatttgag cttccacttc tggatggaaa gattcccaag ccgtttttaa
    24361 atcttcatcg gtcaatttga tatgtttctt tagaccttct tgaaaggcta gactttgttt
    24421 gatactgtct ttgaatgttt gttctgtgta acctgcagat ttcaattgtg aatcaaatga
    24481 atcacccaat tgtttctttg tttgatcata ttgagaatcg atttctttgg ttgatacgtc
    24541 atctccatat ttatcttcaa atacttgata aacgatcaac tgttgcacag cggaatctgc
    24601 tccttgcatt tttttcaagc tatccattac ttcttgttcg ctgattgtac cgcctttata
    24661 actaacggct gttttttcac cggcattcgt acagcctgac aataagccag tgcctagtac
    24721 gatggcaagt gctaaaaatc tttttttcat ttatcaattc tcctactttt cgttttttgc
    24781 gaattgctaa aaacaaaggt gttcaagatg aaaatcatca agaacacctt tactaatctg
    24841 ctcgatacgt cctgaataga tataaggcta cacaatccag aaagttcgct cagcagaata
    24901 aatgtcaagt agcacatcac accttaatat actacttctt ttgttaccgt ttttcaagtc
    24961 tatcttgcgt tccgtattta acgtaactaa ttcttagaac aaatgttatc gattttgttt
    25021 ttcttcttcc gctgctggga tattcaaatc agacactttt atgttcgatt ttccagacat
    25081 ttttttgttg tttttattat gatatatttt tatacaacta aaaatgaata tattaaaact
    25141 accaaaaata acttttaacg ctgtttcatt atatgttttt tctattttat ataaactttt
    25201 ggatacttaa taccaaccgg ttggattgta ccaacatgag aatgtgtcag ttctatttcc
    25261 tgtcgcactt gcataggccc atactccttt tgttgcattt gctgatcgaa tagagtttcc
    25321 aactttcgct tttgcaccgt gacgattata gacacgacta tagtggttag aatgacctgt
    25381 ttttctcgta caagttgtat cactataata aacccatctt ccagagctat gattaattgc
    25441 tgttctagaa atttcccggt ctactggtaa atttgactta ataatattac ctttagcatc
    25501 aaaatgaatc tctcctggct ttaaagattt gtaatagatt tcaccgtgaa ctgaatttgc
    25561 ttccacttca gatgacatag cgtttcctcc aacaaaacct atccctagtg cacaaataat
    25621 aactgctttt ttcataaaac gacatccttt cctttttgat ttttaaagca tgatcttctt
    25681 gtttcctatt ttggtcagat atggatcatg cgtgactacg ataactaacg taccttggga
    25741 agcttgttgc gataagattt caactacttt atccttattt cttttatcca atgacccagt
    25801 cggttcatcg gctaaaatga ttttgctatt cttcaataag actctcgcac aggcgattct
    25861 ttgcttctca ccaccagata atgtgtagat aggcgtactt agcttcttgt ttatatttac
    25921 ttttttcaat gcctcatact ttgctgttcg cttatctgaa acatttaaat tcgaaaactt
    25981 caatccaata tctaaattaa aatcgattgt ttcatattca atcaacccaa aatcttgaaa
    26041 aagaaaagat atggtatttc tttttatcaa gcgcttctct ctcccagtta attgtttcgt
    26101 ctcttgatcg aacaaataga attgtcctga actgaactga tcattcaatc ctaatatatt
    26161 caataacgta cttttacctg ctccactttc tccaacaaca gtcacaagat ccccagcatc
    26221 cgcagtgaaa cgatccaaat gaagaatcgt tttgtttcga atgatttttt tcgcaccttt
    26281 tatcactatc aacggctcca attgttaacc tccattcatc agcaataact gacttttctt
    26341 ttctgtagac aaaataaaaa caactgaaag aataaaatca atgaagctaa agaaaaaaat
    26401 attaaaaaga tatttaggct ctctttctat aagaaaaagg aagattacac ttgtcgttcc
    26461 ccatttgatg aggttcatca tgaagaaatc tttatacgtt tcaaagagcg aataaccact
    26521 tgaacgaaag atagcgattt tccgacaatt gtagacaaaa tatatgcata acatgacata
    26581 gctcaacaag aagaaagaaa agaaagtgag taaaaatatc tgtatcaagt aatgaaatga
    26641 tctgcttaac actttgacag ctatctcttc agtctgagta tacggaaatg ataactgcaa
    26701 gttatcacgt aactgacttt cttcaatcag ctcaaataac ctttctttcg acaaagagcc
    26761 tatcttgatc tttaacggtg gatagatttc tccgctgaga atatttcttt ccaaatacat
    26821 actgtttttc aatgtcagta tttcaacaac cggataatgt tcgatccagg ggatagaagg
    26881 aataaatgta taaatgggtt gattgttttt tataaataga aatttcggtt ctttttcact
    26941 gatttcttga taatattctg ttatttgcgc caggtcattt tttaaatctg tatgtctata
    27001 tcgttcaggg atcaatataa cagggtgcgt ttctttttcg gttatttgga tcaaccttcc
    27061 agagacatca atcaaaggaa atttcttaag ataattcaga tttattttta tccctctgcc
    27121 aaactgttca ttttcttcat tcaagtaatc tgatacattg actaatatac ttccgttttt
    27181 attcaaagtt tcatataatt cctgatctgc tgctgatgca aaacgattgg aataaataaa
    27241 atccacgtgg tttttcccga tggttactgg ataaaataca tgatagtcct caccagcgat
    27301 ttcttttggt ggttcatggg aaacagctaa ctgtgaagaa gaaaaaacga tagttgctaa
    27361 ttcgacattg gcaacggtca aaagaagaga aaacaacaat ccaaatgtaa aaagaatgta
    27421 ttggatatag ttgaacgctt ctcttaacga agcgattttt tcaactaata aaagagaaat
    27481 caaagagata ccatatattc cgctgaacaa gaaaacgtac atctggaaca aataaagaaa
    27541 ttgctcctta taatacataa aaataaaacc accaatgatt cctgcaatta tgaatgtaat
    27601 aatgattttc tttttcccca ataaacacca caatacttga tgtaatgaaa agtcattatt
    27661 caacaataaa ctgatttctt tattcatcaa taagaaaaag gatgataaca aaaggaataa
    27721 aagaatcaat ccaatagtta aatacactcg ataattatat acgtctgtat ttgaaataac
    27781 cgaatacgaa tagctatttt caaagtcttc aaaaatatta gtggcataat cagtgtgaaa
    27841 tgcttgattg aatcgatcag tgaatagtga aatgacccgt tcataggctt ctttttctct
    27901 cgtcatcaag aaaaattcac cttcaaaatc ttttattttg atcgattctt caatggacgt
    27961 tacgcgaaag gaggtattag aatagggtac cttctcgtga gaagacttga catccccttg
    28021 ataatacaat gtcatttcaa gctttggctg tatcttgaag ttccaagatg tatctacttc
    28081 attggattca tttactgttt ttttcatata agaaagctca gtttctgatg agacattttc
    28141 aaataccatg attagctgct catattcttc ttgcccaata aaatgttgtg gcaatcgaat
    28201 cggagtataa gtatctgttt gatggaaaaa ttcatttgtc cgatagagta cctgtttatt
    28261 tatactaacc aaggcaaaac cactcatgat accggtcact atgattgtga gtaaaactgt
    28321 gaactttttt ttgattatca ccactctccc ttacttaaat gattctttga taaaatcata
    28381 ccgtttttca tcaaaaaaac gtatctatca agtgtatagg tcatgtagaa taaatctata
    28441 tactgtagaa tcgaaaggag aagaagatga acaccggaga aaaaatacga acttaccgca
    28501 gaaattgtaa gcttacgcaa aaagaattag gcgaaaaaat tggtgtttcc gataaaacga
    28561 tttctagttg ggaaaattct cgtacaatgc cagactttga aatgctgtca cttttacatc
    28621 atactttagg tctgccgatt gattttcctt ctattgaatc agctaccgaa tcaatagaac
    28681 caacattgcc agatgaacct cttgttaaca agaaatctta ttcatcaaaa caacccttct
    28741 cactcaaaaa gacactatct ttctttctgc tcgtcttttg tttggtctat ctttttctta
    28801 tcaattatca atacaaaaat gaaacgatcg atcgattcaa tccttttttg aaaccacaaa
    28861 tcggctatac cacactccct gtaggatacg tggacaattc catcgaacgt tgggtatgta
    28921 atgatatctt tggccatggt acatggctgt cttttcacgc tggagaggta gaaaaagaga
    28981 aaaaccatgc tgtaatcaaa cacaaaggaa aacacgtcat gaacgttcgc ctgctcactt
    29041 gggaagccat tccctcagat taccaagaaa tcctcagcca agactatgac gacgccttaa
    29101 aaagaagccc tcaggagcca gataggcatt aagtaggaga tgaacgtaga gatagaaaaa
    29161 atcaatgcac atgatcttat ccaaaaaaca ggggtacaaa ttaattgaaa aagccttgag
    29221 atgaaagttc ctaaataaaa aagttcagaa tgataggtat ttctggctta tagtataata
    29281 tttacgccac agcctcccaa tgattctttt tggaatcata atcaaggatg gacattcaca
    29341 aaaatatttt gctcattcga tccgaataga ttaacgacca atctagtaag taatatagat
    29401 tggtacgtag tcaagctgca cataatttag atcaatttta gttctttttt atatttctat
    29461 gatctgctca tttactttta tatgatggct tacagtaaca taaataaata tgataatcgc
    29521 tggtagttta tccaagagta ctcaataaaa tacaaaatgg attgttcaag cgcctatcat
    29581 aatgataaaa tgaaacaaat aaacgataca aggaggaaga gactttgagt aatggcagaa
    29641 caattttttc attgtaaaaa ttgttcagtt gttaatctat gggatttcgg tagcatgggc
    29701 atttttttat agaggaatca caccaacctg gccgttttat ctcttgttag tgggaatatt
    29761 agtaggaaat tttgcccctc ttcactatcg tacgaacttt tttccaactg attctggcct
    29821 ttttcttaaa acaaagacgc gaaaattaga aaacattatt gaaatgacaa ttacctgtat
    29881 aatcattttc ttagttatat tcctaaacgt cttctaataa gaatctctta aaattagctt
    29941 gttacagtta gttacctagt ttatataaca cagttatctt atcttcctta tcaacatctt
    30001 ttgccaactc tttgtcttaa attcgaaaca tataagcaga gctacgaaca ccaagactgt
    30061 ttcggctctg ttttttgcat caaattcact cttatatagc tgataatctc ggctctttaa
    30121 aagaaattat tttcgcttca ttttattata aaaaggctag gaaccttgat gttatcaggt
    30181 tcctagcctt tatttaagta ttaccccaca gacccttcca tatagatttt taaattacac
    30241 actagtttaa actaaaacaa acaaggaaaa acttaccatt taatcaaaaa attagctcat
    30301 atgaatatat tagaatgtaa attagactgg tcaaaatctg gtcaaaaaag aggacgaata
    30361 caggtagtcc tcttttttgt tattttgata ttctaaatca ttttttattg gattattccc
    30421 ttgttcgata tagacccaaa ttgatctatc aataattatc aaactgagtc atatagagac
    30481 aaaaatttca aaaatgacct gttaaatggt catcgaatat catttaaaat caatacgacg
    30541 gagtcagaca cggagtcaaa acaagcccgc tgagagtaaa aaatatacag tcccataggt
    30601 aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat tccatttaat
    30661 ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc tcaataaact
    30721 attttgggtt acatggtatc tgaaacagct aaaaccttgt cattatagca aaaaatcact
    30781 ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa acaaaagaaa
    30841 aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg gtcatataac
    30901 aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata gctgtcttaa
    30961 atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc tacacttcca
    31021 cttgactacc atctttgaat cgtacaattt tcttaactct tctcgtttac ctcaattcat
    31081 tctcaagtgg aatcaactca tcatctaaca ttttacgcca tgctcgcttc atttcttgtg
    31141 ggtatttaaa tggcttcgct tctggatgtt tggctttata tccttcgtaa tccttactgt
    31201 tgataattgc tttcatatta ggaattggat cttcacctgg tagatattgc agattagaag
    31261 catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc aaatcatcac
    31321 tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta tctaaagtcc
    31381 ccacatcaaa tgcatttaaa atttgtcggt aaacttcttt gactccttct gttttattca
    31441 atttagccaa agctttttca atttcttctc gtgcatctga actattcgca gcataagttc
    31501 ctagtaattg atcaatataa gttgaatcaa tatcatacaa cttagtggta ttttcactat
    31561 aaaattcgat cgttgaaaaa tcttctcctt ctttttctgg ttcttcctct attaatgaac
    31621 ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga ttgttcaaag
    31681 ttggagattt caccatgtca tcattgtaat cgtcataagt cactaacgat tcataagcac
    31741 gattcaattc ttgaaatgct ttgacgtact cgatacggat ttcaagtggc gtatgttcat
    31801 caatggattc catggatgaa acctctttta acttaccata tgcttgtttg aatctttttt
    31861 tggattcctg ataacttgga taaatcaaac ctgattcttc ttttgctttt gaatacagtt
    31921 ttgtcgcatc ctcgacattc ttctccattg tcgctggctt acgaaacgtc acaatcaatc
    31981 cttttgtctt atcaggatac gtacgattcg tacgtgaaaa agcttggatc aatcctgcat
    32041 actctagatt acgatccaca aatagggttt gaatcgtagg ggcgtcaaaa cctgttaata
    32101 gacgatccac tacaatcgct aaatcgattt gtttcccaaa ttgtttgaat tccgctcttt
    32161 ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct aatgaccagg
    32221 aagtatcata atactgatta tattcctcaa taattttttg catttcttct tgttgggcac
    32281 ttgaatctcg gctattttct tctaatgaat aggtaatagc gattcgtgga aaatcaggat
    32341 cctccatcgt tcgtccttca cgaatgagct gattgggaaa ctcttttgtt aaccaatcag
    32401 gctctttcgt catctctttg atggcccgat aatacttttt ggccatgtca atggaactcg
    32461 tggttaagat tgcagatttc gttggcctgc catttcgaaa atcaaatttt gtataggcat
    32521 tatctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct tcaaaaacag
    32581 ctgggtccaa ataactttct tttttgacag gttccatttg atcaatcata cgattgatct
    32641 gttctgatga atactctgca tatgtttcta cttcttttaa tttccgatag attttattct
    32701 ctagtgaagt cggctccacg gtgttttcat gctctacttg aaatcctaag acagagccat
    32761 cttctagcgc attcttaatg gtataggtat gcaagacctc tccatactga tcatgggttg
    32821 tacgtgccaa ttgccctttg gcttgtttcc gattctcagg aaaaatcggt gtacctgtaa
    32881 agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt tcagcactta
    32941 acgctcgatg acactcatct actacaaaca cgatatgttg gcccattaat ttttggaagc
    33001 gattggttcc ttttttctct tcttgtttct ttgcatattt caacgccgca tctagttttt
    33061 gacgggttgt aatgattaca acatttgcat tggcatctgc caacagggtt tcacttaatt
    33121 ctttggcact tcccgttcca acgatcaagc tattcgcttt ggcgttcccg gaagaaatcc
    33181 cagtattaaa ttccgaagca aattttgtaa actctgtggt ggtttgatta tctaaatctt
    33241 tgcggtccac taacatgatc gttcgatcga tccccgattt tcgtgctaaa agcttagtag
    33301 atacaaaact agtcaacgtc tttcctgaac cagtcgcatg ccacacatac cctgattgat
    33361 gtttatttgc ggcaataaac aaggcttgaa tcgcatgtac ttgataagga tgtaacacca
    33421 tgagggtttt attatcttga tcttcactga cgattgtata atccgcaatc aaacgatgag
    33481 catccgggat atttaacact tgtttacaaa actcatataa gttttctact ttttcattgt
    33541 ctttcgttcg ccaactaaat aaaaacttag ggtgcaagtc tttcggtaag gcatttgcaa
    33601 aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa aagatattgt
    33661 tccgaaacat accttcttct gcatattttt tgatttggtt aaatgcctga taaacaccat
    33721 cttttgcagt tacttgtttc agctcgattt ggacaatcgg taatccattg attaataagg
    33781 tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca atttgatgga
    33841 ccacttcata agtagagatt cctccaccaa tgtcttgatt ggaataaagg ataagtgaga
    33901 cagaacccaa ttgcgaatct tcccgttcaa tcgtgatccg agccatccca ttttcacctt
    33961 ttaaccattt cgcagcatca aatggggttt tcgttcgcag cagtaattcc gttttgatcg
    34021 tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta tttgagataa
    34081 ttttttggcg taaattgacc caaaggtctt cttctgattt tagatccgga cgatacgtcc
    34141 actgattatg cccttctcca agtacacgaa tcaactgttc ttcgacttct gcttcctcac
    34201 ggtgactaat tttcattctt cttccctcct agacaaacat cttttgtaag aagctttttt
    34261 tcgtttcttt tagtaaatct aacttacgtt gatgaagagc gatcgtatca tctagctgtt
    34321 tgaagaaagt accgattttt tgttgttcat cctttacagg aaccttaaca ctgatattct
    34381 caatatcaga actgttataa gaaggcaatg cccctacttg ggagtatctt ggcaaattta
    34441 tagtttcaaa caatatttta ccaaaataga tatcccaagt attattaaat aaataagcca
    34501 tggtattgtt atctattaag aaagggcttg tgaccaaacg ttttctatta agcattattg
    34561 ctgcgccaac tttagcaaat ataatcgctg gcacatcttt tattggcttc cattttttac
    34621 gctcaatttg ctcattagat acataatgat ttgcatttct catttcatac tcattaccaa
    34681 tgttattcat gtcggaaact ttaaaaaatg gagtaccttc agaaccacct tgctcggcat
    34741 caggaaatcc aattcccgat tgagttttac ctaattctcc caacttacgc tcttcccaat
    34801 cttccgtaaa accaggaaac cggatttctg gcacttttgc tccgttttca gggaacattt
    34861 tttgtaagaa tccttttttc gtttccttca gcaaatctaa cttacgttga tgaagagcga
    34921 tagtgtcgtc gagttttttg aagaatatgc cgattttttg ttgttctttt aaatcattgg
    34981 gaatatagaa tactgtatct gaaacttttt tccagtctga acgaggcatt tttgttccag
    35041 atgtatcatt tgcagccttt tgatatctat cagcttgaat aagagaataa ataaagtccg
    35101 agtcgctctt cttagctcta aaaacccaga aatccccaat cgctacgcct gtaaaagttg
    35161 ccttgagcca attttttaaa tatggacgta actttccata tagaatatca tttggtaaaa
    35221 attcaatccc ttttctatta tcaaatttct ggctcacatc tttattcagt ctaccttctc
    35281 ctgccacgat atcttcaaat tcaacttttg gaagtctact ggagtttgta cttttattaa
    35341 gtctaacaac taaatcaatt aacttacgct cttcccaatc ttccgtaaat cctgggaaac
    35401 ggatttctgg tcgtgtatca ttgctcatgt ttgaacacct ccagcgcacc ttgtaaccac
    35461 atttcgtttt caggtgttgt ttgcagggaa gagatagctt ctaaaagttc tttttctagc
    35521 acttgctgtt cttgtcgtat cttcttgatg tcatttccga catgaaccat atcaattggt
    35581 tcttcttctt cgaaagtatc tacatagcgt gggatattta agttatagtc attttctttg
    35641 atttcatcaa aggttgccaa atgagcgtat ttctcgacat cttttctttc agcgtaggtt
    35701 tcgaggattt tttgaatgtt ttcttccgat agtttatttt gatttttccc tttaacaaat
    35761 tcacgactag catcaatgaa taatacatct cgagtttgac gattcttctt caacacaatc
    35821 accgtcgttg gaatcgatgt cccaaagaac aagttcgcag gcataccgat cacagcataa
    35881 atactgccat cttctagtaa tttttgacgg atgacccctt ctgcagcgcc acggaataaa
    35941 actccgtgtg gtaagacgat ggccatggta cctgtttcct tcaaatgata gaacccatga
    36001 agaagaaacg caaaatctgc tttggatttt ggcgctaatt tcccataacg attaaaccga
    36061 gaatcatcta agaaagtcgt atctgctgac caattagccg aataaggtgg gttcattact
    36121 actgcatcaa atgtgtaagg ttcatctgtt ggccagtcct tattcaacgt atccccattg
    36181 cggagattca tttcttcggc gtcgacacca tgtaaaatca agttcatttt tgctagattg
    36241 tatgtcgttg tatttagttc ttgcccatga tatttaacgt tatctgggtg tgttaagtag
    36301 ttacgtacgt ttaacatcaa tgaacctgaa cccatcgttg gatcaaaaac actaaagaat
    36361 cgtcgttcct tttgatcgag cgttacgatt tgcgccatca tatcagaaac catatgaggt
    36421 gtgtaaaatt ctccggcttt cttccctgct tctgaagcaa attgactgat cagaaactca
    36481 tacgcatccc cgatcacgtc cccatcgtgt tctaaaacat ctacatcatt caatttcttg
    36541 atgacttcgg taatcgtcac atttcgttgt tgctcatctg ttcctagttt ttttgattgg
    36601 agatccacat catcaaataa cccattaaat tgattgtagg tagatgccaa ttgcacaaag
    36661 gctttgttta gatcgttcaa ttggaacgtt gcttgttttg cttgatccgc taagacgttg
    36721 aaaagatact ctggtgcaat tgcatatccc aaaatatcaa cgattgtggc aatcagatct
    36781 tcttttgatt cttcatctga aagtaattca cgatacaaca ttgtttgttt gcttactgtg
    36841 tcatattcct ctaaggattc atctgctaaa agaaccactt gttccaacaa tcgatcggat
    36901 aaatatttat agaaaatcag tcccaataaa taatttttat attctgacgc atccatttta
    36961 ctacggagat tatctgccgc actaaataat ttttgattta attctgcacc caaaaagata
    37021 cactcctatc tcttaattaa agttgctata taccctctta ttttagcata ccccctttta
    37081 taaaaagcaa aaaaaaaaga aagaaactta ttcttgctaa taaactacgc gaaaggagtc
    37141 taacgttgtt tgatataact tagactgatc gttaacttat tttttatatt tctacatagt
    37201 tatctttaga accatttcag atacatatga aacaattaca tatgattttt tcataaaaaa
    37261 acagtactgt ctagtagtat aaaatataca aatttcttaa aaattttttc actaaaatca
    37321 ctacctgaaa cagtctaaaa cacttatata tcaacgttta atgaaactat ctccgtttca
    37381 tttcagatac aaataaatat agtcgggctc caaatccgaa aaaaaatacc cccagtagct
    37441 tatcctaagc tactggagat gtataagtac ggtcatataa caacggggta gggtaaataa
    37501 atgaaaaaga tggaagaatc attattcaat aactttttta aatacttcgt tgtaatcagt
    37561 tacctgtccg ttcttaaata ccacagaata atctttttac ccattttcaa gtaaccgaag
    37621 tggaatgtct ttaccaactt gctctgtcac tgcattactg atacctgtta gattagctaa
    37681 taaatctgat aaagattgag catctgtaga aatctctgta tcagtattta tcgtgattcc
    37741 tttatcttca tttacaagct tccaagtagg tggtatagct gtgtatgttt cgatgaatga
    37801 atcaattctt tcatctatcg tcttcccagt atctacttct gtctcattac tcttagatga
    37861 atcgattctt tcatccatcg tctgctccgt aactgcttct gtctcattat tctcaccgga
    37921 aactctcttc acacccaaac ttatatattg tccatcttta gtgtgcatag ttaaaattcc
    37981 cacgctttta ctaataaatt cactataacc agttctttca atagagactt caatgtattt
    38041 cacactatca tactcaaaag aaaaatagac atgtaaaagc attttgttgt ttacatctac
    38101 ataataatcc tttgactcta cttgagacct tcctatacgg aatacagctt tactgtctga
    38161 tatctctata ccagcatcct tattattaat ctgccaatct ccaagtagct cttctggcaa
    38221 gctagtagtt tcttgtgaac actttaccac ttcactggta gaggtcgaaa cattcacctc
    38281 cgcactgtta gaagaatttt ccaaggaatc aggagcctta gcacaaccaa caagtcttta
    38341 tatattgtgc agccgatatt ttcttcattc agtacaccct tgaaatatgc agtgttagat
    38401 aaagcagttc gtcctgattt gctgaggcac ccatctcttc ttgaacgtac tccttgattt
    38461 tcaaggccgc ccgatactct ttcggaaaac gctgcttcac tgaagccagc aaaaaatcat
    38521 ccttctccgg caactcttcc ttcttaatca ttcgggcaat aaaaaactgt aaatgcgtca
    38581 caaatcttaa gtaaagcatc gaatctttat caaccttttt ttcgggaaac agatatccta
    38641 gcatagccaa gatatccttt agtgctctta ccgagagcat ggtttcttcc atatttgctt
    38701 gttcactttg ggcatttaca atgtgaaacg caatgtttcc agcctcctct tctggcaact
    38761 gaacattcat ttttttgttg atcaattgga tagcatagca gccgatctca aattcttttg
    38821 ggtaatactt ttgaatttca atcagcaggc gattttgaaa aacaatgcct ttttgactgc
    38881 gttcaatggc atgcttcaag tgatcaacca aggtaaaaaa tagactttta ttcagcttaa
    38941 agtcaaactt ggcgttggca taatcgataa ttcgcttgac gcaaataatt agatcatccg
    39001 gagcagcctc aaacagttta atgtaatcct tttttagagc atcagaaagt gaatctaatt
    39061 taaatacttt ttggattttg tcagccggaa ccaaatcgcc tttctttagt tgaaatccaa
    39121 tccctttccc catcaaaatt acttctttgc cttgcttatc taagcaaaga attaaactgt
    39181 tgtttaacgt tttaattact ctcattagat tcaccgttct ctctaagcta agccatttct
    39241 tgcgattact ttttgatacc aaaagaagct ttcttttttt attcgtttta attctttaag
    39301 attctcatcg gttcggtcaa catatataaa accataacgc ttattgaagc cttgatgggt
    39361 actaacaaca tcaatcgctg accaaggtaa ataaccaaaa acatctacac catctgttaa
    39421 agctgcttga atagccagaa tatgtttggc tagataatca attcgatcct ggtcattaat
    39481 tcgtctatct tcctcaacta tgtcgggtgc cccatagcca ttttcagtaa ttaaaatagg
    39541 taaatcatag cgatcgtaca atttcctcaa tgttaatcgt agtccagtag ggtcaactac
    39601 ccagccatat ggcgttttat ctacataagg attttcagct gcccggtaga caccttcttc
    39661 tcccaacatg atctgttgat cccccgctct agcggaaaca tctgatccgt catttttgct
    39721 ggcagcgatg gtggcagttg aataataatt aatcgcaata aaatcgggtt taccggagga
    39781 taaaattgcc tgatcctctg cctcaatttt aggatataat ccacgatcat tcaaatagtt
    39841 ttcaaacaaa taattgtatt ttccccgaac tgctacatct aagaaagacc agcctctcaa
    39901 tgtttcccaa ttatgggctg caattgcatc ttctggactg gaggtagctt gatacatagc
    39961 cgttaaattg attgctggac cgatttttcc tttaagattc aactgatgat acaagtgaat
    40021 tactctagct tgagctaata aaacatggtg attaatttga aagagttctt tttttgaagg
    40081 cagctctcca cttggtggca aaccaatcgc tccaggatga aggatcatcg tgttttgctc
    40141 attaattgtc agccaatagt tcaccttgtc accatactca gtaagcaata cctgtacata
    40201 gtccacaaaa taatcgatag ttgctcgatt taaccaaccg ccctgctcct ctagaaaata
    40261 aggtaaatcg aaatggtaca tggtgacaat cggttcgatt ccctgattca ggcattcatc
    40321 aattacttga tgataaaaag aaattccctt ttgattggtt ttattatttt caatatccat
    40381 aattcgcgtc caagcgatgg aaaagcgaaa agcttttaag cctaattctt taaacagctg
    40441 tatatcttct ttatagtgat ggtaaaaatc cgaggcaact tcaaaattag ctaccccttc
    40501 tgtatgctga tacttgtcaa taatagacgg ttgtttccca tcctcctgat aggcaccctc
    40561 tgcctggtag gcactaatgg aggcacccca taaaaacttc tctggaaatg ctgaaagctg
    40621 tttgtattgc acaaaaatcc tctccttaac taatttgcat caatggttcg tgttccttta
    40681 agcttccgct tgcttcacta attcctgcgt aattttcact gttggtcact accacaatca
    40741 ctgtatcatc gtagccagcg tttctaatgg cagcgaaatc ggcgttgatt aataattgtc
    40801 ctgctgacac ctgatctcct gcttttacaa atacttcaaa gggttcccct tttaaattca
    40861 cggtgtcgat ccccacatgg atcaataatt caataccttc tttagaacgt agaccaatcg
    40921 catgtttaga ttctaaaata gtaatgactt ctccctcaac aggtgaataa atgctgtttt
    40981 cttctggatg aatggctacg ccctttccca atagtccctt agaaaagaca gcatcgttca
    41041 cttcagaaat aggacacacg gttcctttta ccggattttc aacaatgact gcttcggcta
    41101 ttgccgtttg ctctgattca tcagaagtat cgtcaaatcc aattagataa gcaatgataa
    41161 aggttgcaac aaaggcgatt acacacgtaa taattgcgtg aacaatattc attggatttt
    41221 ctccaataaa ggctggtaag gctgctaagc caggggaaac aaaggcatat ctgactaaac
    41281 cgctcaagcc agcatacaaa ccagcaattg caccaccaat catggaagca actaatggtt
    41341 ttttcagctt caaggttact ccatagagtg aaggctcagt aattcccatt aaagcagtga
    41401 tcccagtgga cacagctagc tgcttcaact ctttgttttt agttttcgcg gctacagcaa
    41461 aggtagcacc tgattgagca atattagaag ccagcattcc tggcccattg atcatttcag
    41521 aacccgtact gctcaattgc atagtagcga ttggcgtcat tgcccaagct gtacccgtaa
    41581 ccaccaaaaa tggctgcaaa ccacccatca aaaatggaat taaccaagct gctttgccgt
    41641 taatccattc agcccccatt gctataagat cgttcagcaa cgttccaaac ggtccaatga
    41701 caaccaacgc taaaggcccc ataattaaaa ataccagcat cggcttagta aagaatttaa
    41761 taaacgaagg cgaaattcga tccgcaaagg gctctacata ggacatgatc caaacagtaa
    41821 agataatcgg taaaactgat cccgcataat ctgcagctaa taccggaatt cctagaaaag
    41881 aaacggcatc gccagtagaa aaaagctgtg aaatatttgg atgaagcaaa atgccagcaa
    41941 cagtaatcgc caaaattggg ttgacatcaa actttatggc tgccccatag gctaatagta
    42001 ctggcataaa ataaaaggct gcatcagcaa tagtattcaa aatgatgtaa gtttgactac
    42061 tgtctgatag tacaccagtc aaagtcaaga cggccaaaat ggcttttacc atcccggcac
    42121 ctgttatagc tggaatcatc ggtgtaaagg tggtagaaat gacactaatc aatcttgaaa
    42181 taatactttc ttgattgtct gaagaggggc cctttttaat tgaagttccc acttcctttt
    42241 gaatttcctt gaaggctgtt tggacttcat tgccaatcac cacttggtat tgtccaccgc
    42301 tatccaccac actaattaca ccaagtgttt tttctaaggc tttcgtatcg gctttccttt
    42361 tatctttgag ttcaaaacgc agtctggtgg cacaatgggt taagctgacc acattttctt
    42421 ttcccccaac ctgttgcaat acatttcttc ctacttctct gtaatccatt attctctctc
    42481 cttaaagcag taaaaaagac caagtcaaac cacgccccaa tcaacattga agtgttagtt
    42541 tgtcttggtc ttgcctgcat aatcagtaac atgccaataa cgttattttg ctgtaatcca
    42601 ataatagcac attatgatag cgcaatcaac agggaattta attttttatc ttttcgtttg
    42661 accgtatagg gtaatataac agttagatca catcaactag ggtatatctg cgtatactta
    42721 ctgctaaaat aagtatatct aatattaact ataccagtaa aaccattcct acataaccag
    42781 cgggaaattc ccaaaatcgt gaaagcatca aaaatgcata taacggatca cacaacttca
    42841 acaattctgc tcttgggaaa aattgatgaa tttggtgagt aaaggatgga ataaacagca
    42901 ttccaacaat ataggcaaca atagtgatac ttgttttttt ggtaaaaatc agtaagcaat
    42961 agccaatttc aatcattccg agatataaag gtatcaatag aatgatttgg gctagtattc
    43021 ttaaaaatta actagcaaat gtttaaactg atttatttct aatctacatg tagtaagtat
    43081 ttcaattcca ccaattatca ataaaaatac caaacatatc cataataatt ttttcttttt
    43141 catttctttt atattgttat aacagtgaat aacataaaaa ttcccgacaa tcattgtaat
    43201 atttttttgt cacctgtatt agtatttaat ttataggaaa ctataaaaac tccctagtga
    43261 atttacacct ttgggagttc ctgtttggac ggtatgcata atacatttaa atcaatttaa
    43321 cattaacacc tcagcatatg gtatctccac atatatacgg tctttctttg gatagtactc
    43381 aatatttatg ttgatagcac ttccattgtc taatatagct ttagcccggt atgtgttatc
    43441 tccattttca tgagtaatcg tcacatcatc acatgatcga tttaagttgt actgttcttg
    43501 taaaatctgt gttaccaatg gttttgatga cttctctaat ccagatgtac ctgtcatttt
    43561 tgaaaaaata atacctataa tagccaagac aacaatacct ataatgacaa acttcttgtt
    43621 catatttact ttattcaact ttttatctcc tatctccaaa ataatttgtt cttatccctc
    43681 ttatcagtac attgacatcc ctaaaagtac accaaagatt gctccagcta cccctgctat
    43741 catcaaaatc gtacccattt tctcatcttt ttctcgatat aagtagccca taataacagc
    43801 aacagcacca aatacaacag gaataaatgc taacgatact accatagaaa tccaaccaat
    43861 aatcaaatac agcttccctg tctcttgtcc atgtacgatt tgttccttca tttcattaat
    43921 tgtctgctca gtacttttct ttactgtgtc tgttccggtt gtctcatctg tttcttgtag
    43981 tgctccacaa aactggcaat acttactttc atctgttatt tctttgccac attttctaca
    44041 atacatgtct tctcctccta aaatcattca attgcttatt agttaactgt ttactttatt
    44101 aaatacatca gaaaacagct cattcattgg tacacttaat atctcagaaa gcatcttcat
    44161 ttgatagggt ttaggaatcg ttctccctgt ttgccacgaa cttacacatt gctgtgtaac
    44221 attcattttt tttgctaact ccgtttgaga aagtcctttt tcttttctgt atcggctgag
    44281 attacttact tttttcatga ttctcccctc ctgttttatt atatacaaca atatgatgta
    44341 tttcaactca taaaatataa aaaacttcaa ataccattac aaatacaaca acttgttgta
    44401 ctatactgag atgggggtgt attaatgaaa cagaaacaat atgcaaaggt cattagagaa
    44461 aagcgaaaag ctctcgggtg gacacaaaaa gaattagctg aaaaaatctt ctctacacaa
    44521 caggcagtag caagatggga aaattctgta acagagccta acctcgatag cttaacagct
    44581 ctttctagag ccctaggaac ccctgtaagc cattttctag acaatgttgt cgtagattat
    44641 gaggaagagt ttttggctct ctatcgttct cttagcactg aagatgcagt tcgaacaatt
    44701 gactatatga agctattaaa aaggcaagaa aatgaacgca atcagctatt gaaagattga
    44761 aaaataaata acccaccgaa ctttaacggt gggtgtattg agatgaaata agattaagag
    44821 ctatggataa cctgtctagt ttaatacaat tgggtctttt ctcttcctta ctctccgtcc
    44881 atcatctctt gttacacttg taaattgata cagaggaact atcccagctc ctgataatat
    44941 caattctttt attgtttcat ctatccctgc ccctttcgct aaatatatta aaaaactact
    45001 acttgctttc acattgtata gtttcttaat atccttagaa gctcgttcaa gagtgaatcc
    45061 ttgtttaaca aagtagcctc tgttttttat taaagcattt ttcacgctgt tatccccaac
    45121 attacgtagg cgttcacaaa cagtgttaat aagttctttc tctgtcatta gatatgtccc
    45181 ttgccacgat aagccctgta aaacttcatg tgcttcatct aaaatcaatt gtttttcctt
    45241 gctgagttct gatcgggaat tttgaggaaa tactctttta gcctcttttt cgttaaaaca
    45301 tatcttaacc cattcataag aaaaatattg atatgtaaaa tgaatttttt ccagaagtgg
    45361 aaggatatct tcattcaatt tttgaatcac agaagttgca ttaaaaacgt ctaatcgata
    45421 gacattgctt ctcttatgat taaagtttcc taacgctttc tcttccttta acctatttag
    45481 gtattcgagc atcttcaaag gaacatcttc atcatctaat ttttttatca atcctataaa
    45541 agtcataagt gatagtaact tattacattt atctatgtca taagttttac catagtaaga
    45601 actcattttt ttagctaatt gttcccccga caaaatacaa gtcacacttt ccttactatc
    45661 atcaaaaaaa ttaacatcag aaagcaagac atcatagaca cgtcgcattt cctctagata
    45721 attccttatt gtcctgaaaa gtttgtcttt cattgattca agagtgtcaa aaagtacttt
    45781 tgattcctct ttcaattttc gaaaatgtgt aggatacaag tctaggttct ctaatagcca
    45841 attaaatgtt tctacccgac tgtctttctt taaaattagc atcacaactc caataaaatt
    45901 ttcactgata tcacaattac tggaatgaca ataaaaaatg gaatgcccag ttttactggt
    45961 aaaaatacta gcagagggat tatgatcgtt gtgaaacaga catcttaagt ttttacttga
    46021 cttaagtaat ttattcatat caatggttaa caatctttca taaatctcat taaaagacat
    46081 gtcggaggaa tcaaacaggc agtctttaaa ccactgtttc atttcttctt tgtcgtcatt
    46141 ttttatcaac ttaacaaaat cggtttcatt gactagttca tactctctcc ctgaatcact
    46201 ttgacaattt ttagaacatg tcatggtaaa atcaatcgga agctcagtaa tatctaacat
    46261 gttgtcttca ttaattatta tcactgcatc tgacttatat cctccaaaaa acattctatt
    46321 cgaagcattt acattgtcat cacacccggg gatgttcttt ttcaagtatt cgtaaacagc
    46381 aaccatttta ccatagtcag taattgcttc ttttaataga aagacaatct tgtatttttc
    46441 tagattttca gagtaccgaa tacttttttg tatcgcaaac gcagaattat gaactaaatt
    46501 gatagataaa accttttcta tgcttaagta tctactatca tctttactca aatctactac
    46561 cttccccttc tcatctttaa cagtgttatc gacatccaga ataattactc tggtttccac
    46621 aaaactctcc ttgtcattgc ctttattctt tgtcttgctt ggaataaatg attgtgatga
    46681 atttagtatc tctacaaact catctcttgt aacttctcta tagttatcta aacacctatc
    46741 tcttacatca gttttttctt tttttgtaac tggcttctct aataattttt cttgatctac
    46801 ataaattgga atattaatca ttgatgtttc catttgtgtt tcctccttat aaaattctgt
    46861 taaagactaa aacatacaga taataaaaac aaactcttta ttactatatg ttttagtctt
    46921 atttgtttac tcaaccttta taccgttcca tgaaactatc aatatcagat tttttaaatc
    46981 taatgaccgt atcaattttt ataacaggta acccgtattg agaaacatat tttctaatcg
    47041 ttcctactga tacacctaga tattcacttg cctgctgata gtccatatac tctagagtga
    47101 ctccagaaat aatgtcttct agcataggct ttagcactgt aattatctct ttcacttgtt
    47161 gttcaacttg tatagaaaca atgctatcaa tgatttgtag agacatattt ccccctcctt
    47221 tcgtgattgc ttgaacaaat tttctaagaa ttatcttcgt attttttgct caaaataaaa
    47281 gtattttgtt attaaccgat taatcaaaca aaaaatgatt accagttcat cgacatacac
    47341 ataatacctc gataaaaaag gggtgtcaac agctttgtaa aatttattta caacacccct
    47401 tttttgaggt atactaattt taggaggtgt ggtcaatatg ataaaacgtc caccaataaa
    47461 ttatttagaa cgaaaaaaga tactcggaac aaaaataaaa gctataagaa agagtaaaaa
    47521 attaacacaa ccagcatttg gtcttatgat taacaacggt caattaattg acaaaaaaac
    47581 tatctacgaa tgggagaagg gaacctattt accaatacca gagcgattat cccgaattgc
    47641 tgatttggga aatatgagta tagaggagtt agtttgtggc aatgtagaag aatatatttt
    47701 gggaattatt ctttatagag atagcattgt actagatggt ataacatttc cagataaaaa
    47761 cctgttccag catcttcgtc aacagttccc accagtacac tctaatttag atacatggct
    47821 ggatagatat tccaaattag aaccagagat gcaagaattt attgctaaca agacttgtaa
    47881 taaagttaaa aatgaaaaaa ttagtctttt caatatctta aaaatagaag aattattcat
    47941 caatgctatt gtagaagaat ttgacaataa cattcttttc ctcactagca gtatagaaga
    48001 actactagaa agaatggtag atgaatggct acctattcaa ttgaaagaca tgtcttatcc
    48061 cgaagaagct gtgagagaaa ttactgataa tataaacaag ctagaacaaa cgatttcatc
    48121 aattgggaaa aaatacacta agaaaaaaat gaagggaggt gataccatct agtggtaagt
    48181 actccttgta aactaagagg agatgattta atggcaaaaa tatcaaagta caaaaagaaa
    48241 aacggtcaag tggcgtggat gtataaagga cacattgcca ccgatccacg aactggtgaa
    48301 aaaataaata ctacgagaag aggttttagt agcaaacaag aagctcaaaa agattatgac
    48361 gagtacagac atcaaatatt atacggtgtt aaaaagaaag cacctgatat gacatttgaa
    48421 gacctgtata atgaatggat aactcatcag cgtaccagtg ttaaagcttc aacaatcgca
    48481 atctcagtaa gatacgcaaa taatcaaata cttccagcat ttggtaagct taaactatcg
    48541 aatatctctg tcccatattg tcaaaaagtg gtagatgaat ggcacagtaa atatgaaagt
    48601 tacgattata tgagaaaaca gactgcacaa attttacgct acggggttgc aatgcaatat
    48661 attgacaata atcctatgga aaaaacactt cttccacgaa aaaaagagta tgagaaaaat
    48721 cgtaaatttt actcaaaaga ggagcttaat aatttactgg atgcttttaa agattttggt
    48781 aacatgaaac aatacgcctt cttcagatta ttggcataca caggtatgag aaagagtgaa
    48841 gtactcgctt tacagtggaa agacatagac acgtttaata aagaactaca tgtcaataaa
    48901 actcttgctg tagatgaatt tggtaaagtt ataattcaat ctccgaaaac aagagcttct
    48961 agacgagtta tctctttaga tactgagact ctttctatcc tcaacaactg gaaattacag
    49021 caaaaagaag agtatttaaa acttggatac aatactagct ctaaagagca acatgttttt
    49081 acaacagtta aaaacactct atacattcca aacactgtta atgattggtt aagatatatt
    49141 ttgaaaaaat ataacttgcc tcgtattaca cctcatgggt ttagacatac acatgctagt
    49201 ttattattag aagcaggaga atctgtcaag gtagttcaac aacgattagg gcatgaaaat
    49261 agtaaagtga cattagacat ctatgcacat attacaaata atgcacctaa gaaaacaggt
    49321 caagactttg ctgatatgat ggctcatcag taaaagagct ggtcaaaatc tggtcatttc
    49381 tagtatacag gcaaaacaaa aaagctagaa cccttatata tcaagggttc tagctcctat
    49441 aacaggttta cccaacactg ccttccatct cataactgat caatcgattg agttcgactg
    49501 catattccat tggaagttct ttggtgaatg gttcaacaaa gcccatgacg atcatttcag
    49561 ttgcttcaga ttcggtcagg cctcggctca tcagatagta gagttgttct tctgagattt
    49621 ttgagacttt ggcttcgtgt tctaaagaga cttggctgtt gtggatctca ttgaatggga
    49681 tcgtatcgga tttcgaccat tcgtccatga tgatcgtgtc acattcgata tgagagatcg
    49741 aacccgcact gtttttggca aaggttactt gtcctcgata gtttacttct ccgccgtctt
    49801 ttgaaattga tttagagacg atagagctag atgtatttgg tgcattgtgg atcatttttg
    49861 cacccgtatc ttggatttgg tttgctcctg caaaggcgat ggagagcatg gtgcctcgtg
    49921 caccttttcc atctaagtaa acacttgggt atttcatcgt tgtttttgca cctaggtttc
    49981 cgtcgatcca ttcaacggtt gcgccttcgt atgcttttgc tcgtttggtt actaagttgt
    50041 acacattgtc tgaccagttt tggatcgttg tgtaacggca atacgcatct tttcttgtaa
    50101 agatttctac gatggcggca tgcaggctgt tacttgaata agtaggtgcc gtacaacctt
    50161 cgacatagtg gatgcttgct ccttcgtcaa caatgatcag cgtacgttcg aattgccccg
    50221 tgttttccgc attgatccgg aagtacgttt gtaatggaac atcgactttg acgccttttg
    50281 gcacgtaaat aaaggtccca cctgaccata cggctgaatt cagcgctgct aatttgttgt
    50341 cggttggcgg tacaagtttt gaaaagtact ctttgaataa ttcagggtat tctttcaaag
    50401 cagagtcagt gtcagtaaag acgattccta acttttcgaa ttcatctttc atattatggt
    50461 acacgacttc tgattcatat tgtgcggaag caccggctag ataagctcgt tcagcttctg
    50521 gtataccaat cttttcaaat gtatctttga ttttttctgg tacgtcttcc cagtcacgag
    50581 cgggtttgtc acttggtttt tggtaatatt tgatggcatc gaaatcgatg tctgaaaggt
    50641 cggggcccca tgtttgcatt ggcattttgt taaatgtttc tagagatttc agacggaatt
    50701 caagcatcca ttctggttcg cctttgattc ttgacatctc acggatcact tcttctgtca
    50761 agccttctcc tgtactgaaa accggttcga catcatcgtg aaacccgaat ttatattctt
    50821 ctaattctgg tacgcccatg ttctcactcc tcttccttgt ggtaatgatg gaaagctgcg
    50881 gtaccatcgt tttccattgc ttttcctagt gctttccagg caagtgtcgc acatttgatc
    50941 cgagcgggaa atttagctac accaccaagc atagcagcat cgcctaattg gtcgggatct
    51001 ttgacttctt ttccttggac aagctgtaga aagtctacag ttaaatgttc tgcttcttct
    51061 attgattttc cgatgacggc gtctgtcatc atcgaggcac ttgccgtact gatggagcag
    51121 ccactgccgt caaaggcgat atttttgatg attccgtctt ttacatcgac ttgcaattcg
    51181 atcacatctc cacatgtggg gttgttcaat tcgatcttgc ttgtcgagtc agtcaagctt
    51241 ccacgatgat gcggatggga agaatgatcc aagatcactt gacggtataa attgtctaat
    51301 ttagatagtc ccatgttgga aaaactcctt tgttgccatg attgcttcaa ctaaacggtc
    51361 ggcgtcttgc gttgtgttgt agaaataaaa gctcgcgcga gcagtcgctg atacttcaag
    51421 gtacttcaat agcggttgcg cacaatgatg acctgctctg acggctaccc cttccatgtc
    51481 taaagctgtc gcgacatcat ggggatgtaa cccatccaaa ttgaatgcaa cgactcctgt
    51541 ccgaaaattc gggtcttgtg gaccataaac agtcaatcca tcaattgctt gtaattttgg
    51601 caacacataa gcggctagtt cttcttcata tcgatggata ttgtccatac cgagtttttg
    51661 caaataatcg attgcatggc ctaatgcgat agcgccggcg atatttggtg tccctgcttc
    51721 gaatttccaa ggtagttctt tccatgtact gtcgtataat tcgacaaaat cgatcatttc
    51781 gccaccaaat tcaacaggtt ccatttcttc taaccatttt cgtttaccat ataaaacacc
    51841 aatacctgtt ggtccgcaca ttttgtgtcc gctgaaagca aagaaatcac agtctagtgc
    51901 ttggacatca atggccatgt gcggggcaga ttgagcacca tctactacga tgaccccgcc
    51961 gttttcatgg acaagttcag ctaattcttg aacaggattg aagacaccta agacattgga
    52021 tacatgagca atggagacga ttttggtttt gtccgtaatc tttttcgctg catcctccat
    52081 atccaggaag ccttcttctg ttagttcgat atatttcaag acagcacctg ttcgttttgc
    52141 caactgctgc cacgggataa cattagaatg atgttccatg taagagagca ctatctcgtc
    52201 tcctgctttg atatagcggt cgccgaaggt tctggctacc caattcaagc ttgtcgtcgt
    52261 accacgagta aacagcactt ctgctgtttc ttttgcatgg atgaacgccc gtactttttc
    52321 tcttgcttgt tcataatcat gggtcgcgcg ttctgctaac gtatggacac cgcgatgaac
    52381 attggcgtta tcgtgttcgt aatagtacgc taatgcctgg agtacttgtt ttggcttttg
    52441 ggtcgtcgct gcattatcca gataaactaa aggttcatca ttgacgtctt gaaataagat
    52501 aggaaaatca ttgcgtagct ttttagcatc gatcatccat tcaacttccc ttcgattact
    52561 tcgactaatt ctttccgaac ttgttctaca gggattgctg ttaagacaga accgaggaat
    52621 cctcgaatca ctaagcgctc tgcttcttct ttatgcaatc ctctactcat cagatagtac
    52681 atttcttctg gatcaactcg tcctacactg gcagcatgtc cagcagttac ttcgttttca
    52741 tcgatcaaaa gaataggatt cgcatcacca cgtgctttat cagaaagcat caagacacga
    52801 ctttcttgtt gggcatctgc accttttgcc cctttcaaga tgtggccgat accattaaag
    52861 gtcaatgttc ctttttctcg gatcactccg tgttgcaaaa tatgcccgat cgtatgaggg
    52921 gctttattgg ttactcgtgt atcgattcct tggatttgtc tgcccgagct gattgccacg
    52981 attttgactt ctgcgtgtgc accttctcct gctaaatctg agtcaaagtc agcgatcaca
    53041 tgaccgtcat tcatgacacc aattgcccag tcgacagaag catctcgtaa aatgtgccca
    53101 cggcgattca tataagaagt aatattttct cctaattgat ctacggctga atatttgatt
    53161 tttgaaccag ctttagcgat gacttccacg atgatattac ctgatgattt tgctacttgt
    53221 tctttggttg tctggaaacg ttccagataa gaaaattcac tatgttcatc tgccacgatc
    53281 aacacatgct tgaagaagtg ctcatcggat gcgccatctt gaatgaataa ggactcgagt
    53341 ggttcatcga tcacgacatt tttaggcaca tacaggaaaa taccgctgtt cataaaagct
    53401 acatggagtg cagtcaattg gtcttcgttc atgtttactg cttttttcat ataatattct
    53461 tggactagtt ctggatattc gatcattgca gtgaacaagt cggtaaaaat gactcctttt
    53521 tctgctaatt ccacaggcag ttgttcaaaa atcgtccatg aaccttgttg gacaagcaca
    53581 ggattgtctt tcattgcatc aaatgccgga atcgtgcctg tttcactgat ggtttgtgtt
    53641 tctttcacat caaataacga ccagcgatgg aatttcaccc gatcgatatg cggcaatggc
    53701 agttcatctg ctttcgctag tgcgtcttga cggaaggttc tcatccaatc aggttcgcct
    53761 ttacttaaag aaaaatcatt taccgcgtca agataatcga gccagttttt ttctttcatt
    53821 cttgctcccc cttacgcttc ttcttcttta tagtcgattc ctaattcttt actgatacca
    53881 gcgtagcctt ctgcttccag acgtttagct aggtcagcat tccctgtcat gactactcgg
    53941 ccttccatca tgatgtgaac gacatcaggt gtgatatagt tcaataatcg ttgatagtgg
    54001 gtgatgatca atgcaccgaa attgtctccg cgcatttcat ttactccttt agcaacgact
    54061 ttcaaagcat cgatatccag acctgaatcg atttcgtcta aaattgcaaa tgtaggttct
    54121 aacatcaaca attgcaggat ctcattccgt ttcttttctc cgccagaaaa accttcgttc
    54181 agataacgtt cagccatttc ttctggcata tttagtaaag ccattttttc atctaacttt
    54241 ttcaaaaatg ccataacaga aattttgtcg ttttcatcac gtttcgcatt gatcgctgca
    54301 cgcatgaatt ccgcattcgt gatacctggt atctcacttg gatattgcat agctaaaaat
    54361 aagccaagtc gcgcacgttc atctacttcc aagtctaaaa catttttccc gtcaaacaaa
    54421 atttcgcctt ctgttacttc atagtttgga ttgcccatga tagcggcaga taaggtagat
    54481 tttcctgtgc cgttaggtcc catgatggca tggatttctc cggttttcat tgttaggttt
    54541 acgcctttaa ggatttcttt ttcctcgatc gacacatgta gattttttat ttctaagaca
    54601 gacatgcatt atacccccaa attctctttc gttctcactc attttagact aattgagaat
    54661 gaagcgcaat gaccttcctt actaaaatca aatatctttt atttattatt aaacaatttt
    54721 gatttctaaa ttagaatagg tataaaaaag atgagaaata agccattaca gacttatttc
    54781 tcatcttttt tgttccatac cttattttgc atcagccttt actggtttaa cagagccgcc
    54841 ccatttgtct gtgatccatt cttggacttc tttgcggtga agcacttcaa tcagtttttt
    54901 gattttgtca ctgtcttcat cacctttacg agtcgcaata atattcacgt aaggagagtt
    54961 gtccttttca agcaatacgg catcttctaa tggattcaac cctgcgccgt aagcgaagtt
    55021 tgcgttgatt gcaactaggt caccttcatc attttggtac gtagaagcta aaagtgatgg
    55081 gtcgatcgta tggttgaact tcaagttctt agggttttct tcaatatcat cgaatgttgc
    55141 tgtttccaga tctactccat ctttgacttt gactaagcca gcatcttgta gaattgtgat
    55201 gatgcgtccc cagtctgatt cagaatttga agtaatgatt gttgcaccat cttttaattc
    55261 ggaaacatct ttgacttttt tagagtacaa gcccattggt tcgatgtgga cagcaccggc
    55321 attgacaaag tcatatcctt tttctttgat ttctttgttc aaataaggaa tatgctggaa
    55381 gtaattggca tcaatgtcgc cttcttccaa agattggttc ggtaatacat agtcatcgaa
    55441 tttgacgatt tcaagatcga ttccttcatc tttcaataat ggtttcacat gttccaaaat
    55501 ctctgcgtgc ggtgtcggtg aagcgccgac tttcaatgtc gtgtcgtctg ctttatttgc
    55561 tgaatctttg ctgtcgctgt tgttaccgca tgctgctaat cctacagtca gcaaaagcgc
    55621 tgctgcaaat ccaaaaattt tctttttcat atcctacatc cccctatatc ctattatctt
    55681 ttgtctgttt tcttagttaa gaaatcaccg attccttgaa cgataaatac gattagtaaa
    55741 ataattaaag tcgcaacgaa tgtcactgtt agattaccac gctggtagcc ttcctgccaa
    55801 gcaagacctc caagtccacc tgcgccgatc gcgccggcca tcgcagtaaa tccgatcatc
    55861 gagatcgttg taactgttaa tccagaaatc aatgcgggaa tactttcagg tatcaagacc
    55921 ttccagatga tttcccagta acttgctccc attgcatctg ctgcttccag tacaccaggg
    55981 ctgacttcac gaaaagcaat ctctactaga cgggcataaa atggtgctgc ggataaaaca
    56041 agagctggaa tggctgcttg cgcacctagc atagtgccta cgattgcttt agtaaatgga
    56101 atgatcaaaa ccatcaggat cataaatggc gttgaccgga aaatattact gatgatcgat
    56161 acgattccat aaatgattcg agcatacggt ttgtttttgc gtccaataga gtaaaggatc
    56221 aatcccaaaa ctaatccgat gatcgtcact aagaccattg agattaatgt catatatagt
    56281 gtatcaagga tagcctgatt catcgtttcc gggttgattt gtttaaaatc tagatatgtt
    56341 tctgaaaagc ttttatccac gatggatcac ctcaattcct actccttgtt ctttgatgaa
    56401 cacttctgct tgatctaatt ctttggtttc accagtcatc aagacagtca atgaaccaaa
    56461 ggaaccttct ttcacttgtt tgatctttcc atacacaacg ttgacatcga tgttatagcg
    56521 tcgaatcgct tgggaaatga ctggctcgtt ggcatttctt tcgctaaagg tcaaagttgc
    56581 caaacggccg cttggatttt ctttgatgag ttcagctaat agttctgtcg aatcagcttc
    56641 tggttcgatg tcttgttgta caaagcgttt ggttacttct tttttcggtt gtctgaagac
    56701 agtcaatacg tctccttctt ctacgacttt ccccaactcc atcacagcga ctttgttgca
    56761 gattttgcgg atcacattca tttcatgtgt gatcaagacg atggtcaaat tcaatttctt
    56821 attgatcgct aacaataagt ctaatacttc ttctgttgtt tgcgggtcca atgcgctagt
    56881 agcctcatca caaagcagga tctcaggatc atttgccaat gctcgtgcga taccgacgcg
    56941 ttgtttctgt ccgccggaaa gttgtgaagg atatgcttgt ccgcgtcctt ctaacccgac
    57001 taaacgcaac agttcttctg cccgttcttt acgtttgctc tttggtacgc cagctaattc
    57061 cagcggcagc tggatatttt caagtacagt tcttgaccat aacaggttga aatgctggaa
    57121 gatcataccg attttctttc ggaatgtacg taattctttg ttttttaatt ttgtaatatc
    57181 ttgatcgttt acgatcactt cgccgctcgt gggaagctca agtccattca ggagacggac
    57241 taatgtactt tttcctgcgc cagaatagcc gacgattcca taaatatccc ctttttcaac
    57301 gtttagtgaa acgtcattga cggcttggat gtttccgttt ttcccattga atgtcttttt
    57361 gacattcgtt agttggatta atgacatgcc attcacatct ttctcttatg atttttcaaa
    57421 caaaaaagcc ttcgccctct tgccttaagc aaaaggacga aagcttgaac ttccgtgtta
    57481 ccaccttgat ttaccgttac ttcgcaataa cagcctcttc cagtacttgg acttagcttc
    57541 tttccgtata ctgtcgcgct gtaacgggca ctcccgtagt aagtttgctt acgctcgctt
    57601 actctgctct aagaccatct tccatctcat tttcgttacc cattttcagc aaacagggct
    57661 ctcttcaaac gtccgtgaca tgtactcttc ttttcaaagc tttctcgttt catataggat
    57721 tgcttatgat tatagcaact ggaagaatca gtgtcaactt ttttcaagtg attttctgat
    57781 tttataattc gtcaaattct tttggatcaa catctttgaa acttaagatc cacgcattca
    57841 tttcatctga atcattcaag atattgatat cttcaccgat tttatcatta acagaagaaa
    57901 ctgtacccgt tagcgggctg gcaaattcat tgaccgcttt ttccgcttca acttcgatca
    57961 gaggttcatc tttttttagc gattgtcctg cttttggcag attggcaaac gtgatgtctc
    58021 caagttcttc ttgcgcttca tttgtcaaac caacacagta ttcttttcca ttaaataaga
    58081 tccaaaggtt gtcctttttc ttcaaatgct tgcctgccat tttatatcag ttctccttca
    58141 taaatttttt cattgaatcc aattgcaaca agctggtcct ctcgtaagat cagtggtcgc
    58201 ttgatcagca taccatctgt cgataagact tggctcgctt ttgaagccgt aaactcgcct
    58261 acttgatctt tcagtccaag ttcccgatat ttcatcccgc ttgtgttgaa caggcgacgg
    58321 attggtactt gactcgtttc tagccaagtc tcaatcgttg aggccttcgg tacttctttg
    58381 atcatatcaa cggtttgata actaacgcca tgctggtcta accacgcttt ggcttttttg
    58441 catgttgaac atttcgggta ccagtaaaag gtgtacaatt ttcttacctc cttgaagatc
    58501 ttgcaggtgc ttcattttgg tatctcatcg ataatatcaa cccgataccg atcatgttcc
    58561 ctaaaatcga ggatcctccc tgactaatga aaggcaatgg gatccctgtt aaaggaagta
    58621 atccgatatt tgctccaata ttctcaaaga catggaacag cagcatcatg atcaaaccgc
    58681 ttgcgatata ggcgtaaaat tcattattcg tatcgaaaca cacccgaatc atacgataga
    58741 ttaaaataaa atacaagaaa atgacgaatg caccaccgat aaagccgaag ttttcaccga
    58801 taacggtaaa aatcatatcg gactcacgaa caggcacata gatattgctt acgttgaatc
    58861 ctttaccaaa caagccaccg gtaccaattg ccaaaagtcc ttgggccggc tgataactca
    58921 ttcctgacgt atcatggaaa gggtccagcc atgaatcaat acgtgcaaac tgataagatt
    58981 tgaatccaac tttataaagc aagtctcttc cgccttcagt cgtaaccaag aagatcgttc
    59041 ctgctccaac gactactgcc aagattacta caggtgcgat gatgcgccaa gagattccag
    59101 acatcaggaa aatcccgcca aagatcgcaa gaaagacaag catcgttcca aagtcatctt
    59161 gggccatgat caatgctaaa accggtattg tcacgatcaa gagcttccca atcagccaaa
    59221 agtccgattt catcgtccgt tcttttactt gcgtattatg tttagttacg atcagtgcca
    59281 tcatcaagat gtacgcaatc ttcatcaact ctgctggctg gaaagtaaac gcaccgaatc
    59341 gaaaccagtt tttagaccct gttgaagcag ctaaattctg atcatagaat ttgagcaaga
    59401 gtcccattac cactaacccc aaaccataaa ggtaaggagt cagtttccag atccatttgg
    59461 aattcaaatg catgatgaca acgattgctg ccgctccaac taaataccag acaacttgca
    59521 tccctacgcc tttcaagact gctggattgt tcggatcatg gctaagtgct acatataatg
    59581 agagcagacc aatcaaacat aacagaaaga ctggtaaaat cacgccataa tcaatacgat
    59641 tatcgttcga agcttttaat tttttttcca tgtttcatcc cttttcttaa aaaccatact
    59701 gctgattatt atagcggtcc agttagataa tggaaagtct ttccatagac atttgggaaa
    59761 aattaacttt ccgtttgtgc agttgcttga gcaaatgcag tacaaaagcc tactgcttat
    59821 ctttagtgta tcatagaact ttttttgata catgcaatta tctaccagct gagattctcc
    59881 tcgctgtgag aagtttctgt gctttcattg tatcgaagga tctcttcggc aatctctagc
    59941 agctgatcag aaaccagatt ggttcgttgg atgaaataaa aatatcgatg gaaattcaac
    60001 ggagtcattg gcacattttc tggaacagag cgcttagaca aaatcgagcg accgattcct
    60061 tccttcagca acgcaacaat tacatcgtta cttttcacga tcatcttctt atcttggata
    60121 ttcatctctt caaaataacg ctgcgtgtaa tgaaaaacac cagagtttgc ttctcttaca
    60181 agccacaatt tgctttttgg gtccccagcg atgactagct ggtcttccat caaagagtat
    60241 ctttgtacgc cggaagtctc taatggcttt tcgataaaac caaaatcgga ttcatgtttc
    60301 gctacttgtt ctaatgattc atgagaattc gtgatttcga tctcaaaatg cactttagga
    60361 aaccttttga tcagatgttt catcaactga ggcaaaagat agacagcaaa ggtgtgggaa
    60421 gcaacgatcc gacatacttc ttcttcattt tgttcattga gcaatttttg ctgagtttct
    60481 tcccattctt ctaacaaaac cagtgtccgt tcatataata agtcggcttg cttagttgga
    60541 agcatctctt ttcttccgtt acgttgaaaa agatcgatat tcaattcccg ctccaactgt
    60601 ttgatttgat tcgagactgc tggttgagat aaaaacagta cttctgctgc tctggaaaaa
    60661 tttcttgttt catatactgc tcgaaaagtc gttaataact taaacatggc accacttcca
    60721 taacttttta tgataattac tataaaaact atttgattta aatatacaaa aaaccatcta
    60781 taatagaaat gtacaacaaa acagttgctg atccaactag taacttagat gaagacaatc
    60841 agctgtctgc ttgctcgtaa tcatacattc ctattcattt tacaatgtat tcgttcatct
    60901 tggtactaaa atctattaaa taaaaaggag aatattatat atgaagaact ttttgaaaat
    60961 tattccgatc ccgatctgcg ggttgatcct tgggttagct tcccttggaa atctgtttaa
    61021 agactatcac catgttgctt taggcaatct aactggcggt atctctatga ttcttatgat
    61081 attcattttt gttaaattgt ttatcttatt cgaacacacg aaacaaacgt tgcatgatcc
    61141 aatcatcgcc tccgtttcac caacttttac gatgtcactc atggttattt gtacttattt
    61201 tgtttcatat gaaagtattg caccaattgt caaattcatt tggctgtgcg ctgttatctt
    61261 ccaagtgatc cttgtctttt atttcaatta tcaccatgtt atcaaagctg atctttcaat
    61321 tgaagcaatt tatccaagct ggtttatcat ttacgttgga tttggggtca tcactgtaac
    61381 tgccgggaac ttcttcccac ttattggaaa aattttcttt tggatttcat tgatctgcta
    61441 tgcgttctta ttgccgatca tcatcaaacg tgtctttttc gtaagaaata tggctcatcc
    61501 gacacgtccg ctgattacga tcgttgcagc acctggttct ctttgcctaa caggttatct
    61561 taaaaacttt gctgaaccta atttttatct tgtcgtagca ttgttcgctt tatctcaagt
    61621 attgtatttc gttgtgctgt ctatgcttcc acgtttgttc aagcttcgtt tttatccaag
    61681 ctatgcagcc tttacattcc cgcttgttat ctctgcaaca gctatattta ctactgttca
    61741 ttacttcaac agtttaggta tctatagtag cgtattggat ttcttaaaag ttgcggaatg
    61801 tctgtttgca agtgtagttg tcttctacgt tcttgctcat tacgtacgtt tcttaattaa
    61861 agaacatagc aaacatggaa aatttgtttc taaaaaaatc gcttaataat cgattcaata
    61921 ggttgtgaaa aagctgttct gcatcaagta ataagaacgg ccaaacaaaa acagctgttc
    61981 atgtttttgt actggttggg cttattaccg cagatgcaag cttttgaaca ccgtttacca
    62041 agaggtcgta a
//
LOCUS       DACEPD010000005        59930 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.005, whole genome shotgun sequence.
ACCESSION   DACEPD010000005 DACEPD010000000
VERSION     DACEPD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.005"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(101..1120)
                     /locus_tag="IW797_000278"
     CDS             complement(101..1120)
                     /locus_tag="IW797_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1223081.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1142..2149)
                     /locus_tag="IW797_000279"
     CDS             complement(1142..2149)
                     /locus_tag="IW797_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1223082.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2142..2807)
                     /gene="pgmB"
                     /locus_tag="IW797_000280"
     CDS             complement(2142..2807)
                     /gene="pgmB"
                     /locus_tag="IW797_000280"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1223083.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2800..5094)
                     /locus_tag="IW797_000281"
     CDS             complement(2800..5094)
                     /locus_tag="IW797_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1223084.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5354..7519
                     /locus_tag="IW797_000282"
     CDS             5354..7519
                     /locus_tag="IW797_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1223085.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7823..8656
                     /locus_tag="IW797_000283"
     CDS             7823..8656
                     /locus_tag="IW797_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ1223086.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8758..10458)
                     /locus_tag="IW797_000284"
     CDS             complement(8758..10458)
                     /locus_tag="IW797_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223087.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10965..12125
                     /locus_tag="IW797_000285"
     CDS             10965..12125
                     /locus_tag="IW797_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1223088.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12141..12584
                     /locus_tag="IW797_000286"
     CDS             12141..12584
                     /locus_tag="IW797_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1223089.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13067..13597)
                     /locus_tag="IW797_000287"
     CDS             complement(13067..13597)
                     /locus_tag="IW797_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ1223090.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13702..14253
                     /locus_tag="IW797_000288"
     CDS             13702..14253
                     /locus_tag="IW797_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1223091.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14406..14879)
                     /locus_tag="IW797_000289"
     CDS             complement(14406..14879)
                     /locus_tag="IW797_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1223092.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15017..16489
                     /locus_tag="IW797_000290"
     CDS             15017..16489
                     /locus_tag="IW797_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1223093.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16486..17196
                     /locus_tag="IW797_000291"
     CDS             16486..17196
                     /locus_tag="IW797_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ1223094.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            17428..17712
                     /locus_tag="IW797_000292"
     CDS             17428..17712
                     /locus_tag="IW797_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ1223095.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(17993..18412)
                     /locus_tag="IW797_000293"
     CDS             complement(17993..18412)
                     /locus_tag="IW797_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ1223096.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(18414..18998)
                     /locus_tag="IW797_000294"
     CDS             complement(18414..18998)
                     /locus_tag="IW797_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ1223097.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(19045..20520)
                     /locus_tag="IW797_000295"
     CDS             complement(19045..20520)
                     /locus_tag="IW797_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ1223098.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            20701..21012
                     /locus_tag="IW797_000296"
     CDS             20701..21012
                     /locus_tag="IW797_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223099.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(21127..23463)
                     /locus_tag="IW797_000297"
     CDS             complement(21127..23463)
                     /locus_tag="IW797_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1223100.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            23605..24273
                     /locus_tag="IW797_000298"
     CDS             23605..24273
                     /locus_tag="IW797_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1223101.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            24276..25319
                     /locus_tag="IW797_000299"
     CDS             24276..25319
                     /locus_tag="IW797_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1223102.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(25556..26437)
                     /locus_tag="IW797_000300"
     CDS             complement(25556..26437)
                     /locus_tag="IW797_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1223103.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERANRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            26596..27324
                     /locus_tag="IW797_000301"
     CDS             26596..27324
                     /locus_tag="IW797_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ1223104.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            27480..27797
                     /locus_tag="IW797_000302"
     CDS             27480..27797
                     /locus_tag="IW797_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1223105.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            27790..28398
                     /locus_tag="IW797_000303"
     CDS             27790..28398
                     /locus_tag="IW797_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ1223106.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            28453..29244
                     /locus_tag="IW797_000304"
     CDS             28453..29244
                     /locus_tag="IW797_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ1223107.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GKSYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            29382..29870
                     /locus_tag="IW797_000305"
     CDS             29382..29870
                     /locus_tag="IW797_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ1223108.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            29921..30793
                     /locus_tag="IW797_000306"
     CDS             29921..30793
                     /locus_tag="IW797_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ1223109.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            30969..31331
                     /locus_tag="IW797_000307"
     CDS             30969..31331
                     /locus_tag="IW797_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1223110.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            31340..32092
                     /locus_tag="IW797_000308"
     CDS             31340..32092
                     /locus_tag="IW797_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1223111.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            32106..33908
                     /locus_tag="IW797_000309"
     CDS             32106..33908
                     /locus_tag="IW797_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1223112.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            34185..35226
                     /locus_tag="IW797_000310"
                     /pseudo
     CDS             34185..35226
                     /locus_tag="IW797_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(35278..37422)
                     /locus_tag="IW797_000311"
     CDS             complement(35278..37422)
                     /locus_tag="IW797_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1223113.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNENTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(37779..39152)
                     /locus_tag="IW797_000312"
     CDS             complement(37779..39152)
                     /locus_tag="IW797_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1223114.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(39152..39547)
                     /gene="rbsD"
                     /locus_tag="IW797_000313"
     CDS             complement(39152..39547)
                     /gene="rbsD"
                     /locus_tag="IW797_000313"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ1223115.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(39575..40492)
                     /gene="rbsK"
                     /locus_tag="IW797_000314"
     CDS             complement(39575..40492)
                     /gene="rbsK"
                     /locus_tag="IW797_000314"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ1223116.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(40600..41613)
                     /locus_tag="IW797_000315"
     CDS             complement(40600..41613)
                     /locus_tag="IW797_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1223117.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(41694..42293)
                     /locus_tag="IW797_000316"
     CDS             complement(41694..42293)
                     /locus_tag="IW797_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1223118.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            42479..43180
                     /locus_tag="IW797_000317"
     CDS             42479..43180
                     /locus_tag="IW797_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1223119.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            43190..45901
                     /locus_tag="IW797_000318"
     CDS             43190..45901
                     /locus_tag="IW797_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1223120.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            46352..47980
                     /gene="treC"
                     /locus_tag="IW797_000319"
     CDS             46352..47980
                     /gene="treC"
                     /locus_tag="IW797_000319"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ1223121.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(48040..49221)
                     /locus_tag="IW797_000320"
     CDS             complement(48040..49221)
                     /locus_tag="IW797_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1223122.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            49473..50996
                     /locus_tag="IW797_000321"
     CDS             49473..50996
                     /locus_tag="IW797_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1223123.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(51065..51793)
                     /locus_tag="IW797_000322"
     CDS             complement(51065..51793)
                     /locus_tag="IW797_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1223124.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(51806..53087)
                     /locus_tag="IW797_000323"
                     /pseudo
     CDS             complement(51806..53087)
                     /locus_tag="IW797_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
     gene            complement(53228..53830)
                     /locus_tag="IW797_000324"
     CDS             complement(53228..53830)
                     /locus_tag="IW797_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ1223125.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            54075..54281
                     /locus_tag="IW797_000325"
     CDS             54075..54281
                     /locus_tag="IW797_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223126.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            54593..55312
                     /locus_tag="IW797_000326"
     CDS             54593..55312
                     /locus_tag="IW797_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1223127.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(55393..56076)
                     /locus_tag="IW797_000327"
     CDS             complement(55393..56076)
                     /locus_tag="IW797_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ1223128.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            56294..58480
                     /locus_tag="IW797_000328"
     CDS             56294..58480
                     /locus_tag="IW797_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1223129.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            58826..59236
                     /locus_tag="IW797_000329"
     CDS             58826..59236
                     /locus_tag="IW797_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1223130.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            59250..59606
                     /locus_tag="IW797_000330"
     CDS             59250..59606
                     /locus_tag="IW797_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ1223131.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT        19255 a        10614 c        12079 g        17982 t
ORIGIN      
        1 ctctttttta cagataaaca atgtgttaga agtagtatct tcttttacat aaaaagctga
       61 ccataggata aatggtcagc gtatttagtg agtcagctat ttagtatggg ttttctaaag
      121 gaacgactgt ctctcttttg atcaagcgat gagggatgac caaacgtacg ccactgctca
      181 attcttcatg gatcaattcg cgcagctttt gcatagcgac acgtcctaat tcggagatat
      241 cgatatcgat acttgtaagg taagggtgtg ttaatgttga gaagatcgag ttgttgaagc
      301 taatgacgga gagtgtctct ggaatgacat agccatacat ttgcgccaac tgcatcgtgc
      361 gaagggcgaa gatatcgtca atgaccacca atgctgtggc ttcgttttct ttcaacgttt
      421 tctcaaagga cagataatcc tccggatgct ctagtactac actggataac gcaggaagac
      481 cagccatcat cattgctttt tgataaccga aatatcgttc gaaatacaaa ctttcatgag
      541 tcgtgtttgt cacaaacagg atattcttat gtccattctg gatcaaaaat tctgttgctt
      601 gtttacctag aagctggtta tcattatcta cataaacgat ttcgttctca tgatgataag
      661 gctgtccgat caaagtgaaa gggatgtggt tttggtataa ataatcaatg acagtatctt
      721 gactatctga atacactaga ataaaaccat ctacttgttt ttgtaaatgc atacgctgga
      781 cgttctctaa cagcaattca aaatttttag cagtagcgat tgctgtagtc atatcataat
      841 tccgcgcttc ttcgttgatt gcttccatga tctcaagata aaatgggtta ccgatccgct
      901 cttttgaatc aagagggggt aagattacgc cgatggcact ggaaatgcgt tttcctagat
      961 tgcgggcagt catgttagga acataaccta gttcatccat aatttttcgg actttcttct
     1021 ttgttgtctc ggaaatacta ggatgatcgt tgatgacacg agagacggtc gaggtggcaa
     1081 caccggcttt tttcgccaca tcttttacgg taatagccat gataaaaaca cctttctctt
     1141 gttattgtgc agagaaccga taaatcgtct gactgatccg ttcttgacca ggatggaggt
     1201 tgatcgatcc aaatttgggg aaatgaacga tatcagggat ttcttgcggt tcgattgcta
     1261 atccgtagtt tgaatgcatc tgttttccat tgacagagaa atcagcttcg aatccagtcg
     1321 tagaaaataa taccatactt ttattagaag ttgctatagt catttggcga ccagaatgct
     1381 tctcgaataa ttgtaaggaa gttttggaaa gcctaggatg atgcaaaaga aatacatcat
     1441 ctagtccatc tggataacat gcaaggatct cttgcagtgt tggggatttt ctaaagtcga
     1501 ttggcagttt ttcttttttt gtccaatttc cagtaggcag tttttcttga tctaattcaa
     1561 gcacaccatc taaaaagacc gttaactgat gttcatagat gtctcttgtt ccatttcctg
     1621 aaaggttgaa gtaggcatgg ttggtcggat tgcagatggt ttcaacaagg ctactagcag
     1681 aggttgtcat ctctaattcg ttttttgtta aacggtaagt gatcgtagca gtgattggtc
     1741 ctggataggt agaaaattca tcttttaagt aaaagaccac acctattgat gatggagttt
     1801 tgaaaacttc tacttcccaa aattggaaag accagccttt cgtgccaccg tggatatggt
     1861 gtgatccgca gtttttctcc aatctatgct caccccaaga agcatcccgg atccttccgg
     1921 ctacaggtcc gacagttgct ccaaaaaagc ttttgtctgt tagaacatct tcgaaacgat
     1981 cgtaagacaa taaaacattt tctgattttc cttctttgtc tggtgcgaag atttggtgca
     2041 ggcgggcacc gtaattgaga agaacgactt ctaagtagtc gttcttcaat gtgattttct
     2101 ttatttcagt ctctggaact ggtacgacgg agatctctgg attatccatg gtcatgccac
     2161 ttttctttca agtaatcgat cgtaagaaaa gtcgtatcag ggacaagcgg gatctcctct
     2221 ccaaggtctt cagcacgtcc cacgccaatt ggttgagcgc cacttgagag gatggcttgg
     2281 ataccggctt gcgcgtcttc gatcccgata cattcttctg cttttagtcc gacagctttt
     2341 gctgcaagga ggaagatatc tggcgcaggt tttccgtttg ttgcttctgc cgggtcggca
     2401 atagcatcaa agtaaggtgt aagacccatt ttttcaagta ggaatggccc gtttttactt
     2461 gcagaagcaa gagcaatctt gattttttct tcttttaaag aatccagtaa aggcacgacc
     2521 ccggggaaga catcttttgg ttcgatcgtc tggatcatct ctaaataata atcgtttttt
     2581 cttttcgcta attctgcaaa ttcttccttt gaaaaagagt gttcttttcc gccataagct
     2641 aaaagcaaag agagtgagtc ttctcggctg acgcctttta gctgttcgtt gaatgtgtgg
     2701 tcgatcgtaa tgccgatttc ctgaccaagc tttcgccaag cacgataatg gaattcagca
     2761 gtatcggtaa tgacaccgtc taaatcaaac aatacccctt taaacatttg cttcctcctt
     2821 tcttagcgtt atttctagac gatccgctaa agaataagac tgttcataga cgtaaatttc
     2881 tagaggagaa ccttctaata actcgaaagt aacttgttca tctgtgactg agatgaacaa
     2941 taaacgtccg cgataattga tatggaaaga ataagctttc caagtttttg gcagaaatgg
     3001 tgcgaatctc aactgatcat gggcagtttt catttgagca aatccttgga caatcgacaa
     3061 ccagctacca gtcatggaag tgatgtgcaa gccgtcttct gtgtcgttat tgtaattatc
     3121 caaatccaag cgagctgttc gctggtacat ttctacagct ttgctttcca ttcctaattc
     3181 tgctgctaaa atcgaatgga tacttggcga aagggaagac tcatggacag tcattggttc
     3241 atagaaatca aagttttttt gcttttcttc ttttgtaaaa gtatcattga agaaataaat
     3301 cccctgtagt acatctgcct gcttgatgaa gcaagatcta aggattttgt cccaagacca
     3361 atgctgattc agaggaagat cgcttgtggg aatatcggat acaggcatga gatctttatc
     3421 taagaatgtg tcatgctgga caaagattcc taattcttcg tcaactggga agtacatgtt
     3481 ctctgcgata tctttccatt tggctagttc ttcttcgctg atagtgatag cagcttcttt
     3541 ttgataagtt aaataatttt ctcgcgtgta acgcaagacc caagctgcga tcgtatttgt
     3601 ataccaattg ttattgatat tgttctcata ttcgtttggg ccggtcacac cgtggatcat
     3661 gtatttattt gctcgttttg aaaaatggac acggtctgcc cagaagcgtg cgatttctgc
     3721 taaaacttcc agtccttcat ttttcaaata ttccgtatcg ccggtgtaat tcgtataatt
     3781 gtagattgca taggcaatcg cgccatttcg gtgaatctcc tcgaatgtga tttcccactc
     3841 gttatggcat tcgacaccag tgaaggtgac cataggataa agcgcgcctt ttaatccttg
     3901 ttgctgagcg ttgtggattg cttgcggcag ttgattatgc cgatatttca gcagattctt
     3961 agttacttca ggtttggcta aagcaagata aagagggaca gcatacgctt ctgtatccca
     4021 gtaagtggct ccgccgtatt tttctcctgt gaatcctttg gggccgatat tcaatcgttc
     4081 atcttcgccg tagtaagtag agaatagttg gaacaaattg aatcgaatac cttgttgtgc
     4141 ttcttcgtct ccttcgatga ccacatcggc aagcttccat cgttctgccc aagccgctgt
     4201 ttgctcttct tttgcttgga tataattgtt tgtctggttc gttaattctg agacagcttt
     4261 ttctgcttgc tgcgcttctg gaatatctct actagtaaga acgataattt ctttttccac
     4321 tgaccatgtc tcaccttgag ataattctgc agacaagcaa ccatgtaatg ctaaagtatc
     4381 ttcgtgatag tcagggatga ctgattttcc attaacgaaa tggcgcattg ctgcagtaac
     4441 agtaaattgt tcaatcccaa agttgtttgg aatagtttta gtcgtcaaat agccgatttc
     4501 ttctatagtt ccacgatcaa tctcattcca aaaatgttca tcatagttgc tgtcttcgtt
     4561 ttgaacattt ccatccaatt ttgatacgag tttgactgtt ccgctacctt caagcatttc
     4621 tatagttaaa tagatataag ctgattcttt tttgacaatg ctcagaaaac gttcgaagga
     4681 aatgcgtact ttattagtag aagtagtgat tgtgaatgta cgagataata caccattttt
     4741 catatctaat tcccagtaaa agtcttctgg ttccaaagaa gccaagtcga tcagctgatc
     4801 gttgatcgaa atatctatcg cgataaagtt caaggcgttg atcacttttc caaagtattc
     4861 tggataacca tttttccacc agccgacacg tgtcttatcg ggataccaga cacccgccaa
     4921 ataagtacct tgatggtggt cccctgagta agtttcttca aagttcccgc gcatccccat
     4981 ataaccgttt ccaatacttg ttaaagattc ttgcagtcga agattttttt tatctaattg
     5041 tgtcgtgctg attttccatg gatcaatagc aaatagtcgt ttgatctgtt tcatttttct
     5101 cctcctaatg agctgtttta tacgttcata ataaacgcaa ccgattgcac aagtcaatag
     5161 acaaaagaaa atatttcata gaaaaaagct taaaaaaata aatttttagg agaaaattat
     5221 agaagggaac acacgtatta taggttttat agaatggtat agacagaaaa ataaaaaata
     5281 aagcatttac aaaaaagaaa acgcacgcta taatacgttt aacgaaaccg attgcgtaaa
     5341 ggagctaaac ggaatgaaaa agatatttag ttttgagttt tggcaaaaat ttggaaaggc
     5401 tttaatggtt gtggtagcag ttatgccggc agctgggttg atgatcagta tagggaaatc
     5461 aattcccttg atcaatcctg attggacgcc gcttgtcaca acgggcggag taattgaaaa
     5521 tattggttgg gcaattattg gtaatcttca tattttattt gctttagcaa taggaggaag
     5581 ttgggcaaaa gaaagagcag gcggagcatt tgcagctggg atctcattta ttttaatcaa
     5641 tcggatcact ggggctatct ttggtgtcac aagtgacatg cttgcgaatg aagatgcatt
     5701 tacccacact ctgttcggta cgaagatcat ggttaaagga ttttttacaa gtgtattgga
     5761 agcaccagct ttgaatatgg gagtattcgt agggatcatc gctggatttg tcggggcaat
     5821 ggcttataat aagtactata attatagaaa attgccggat gctttgtcat ttttcaatgg
     5881 aaagcgcttt gttccgtttg ttgttatctt atggtcaaca atcgtggcat tgattcttgc
     5941 tgtggtgtgg ccaaacgtac aggctggaat caataatttt ggtttatgga ttgcagaatc
     6001 acaagaaagc gcaccgattc tagcaccgtt tttatttgga acattagaac gtttactatt
     6061 gccatttgga ttgcatcata tgctgacgat tcctatcaac tatacacagc ttggcggatc
     6121 gtatgagatt ttatcaggcg cacaggcggg gacacaagta tttgggcaag atccgttatg
     6181 gttggcttgg gccactgatt tagtgaatct taaaggtgca ggggatatgt cgcaatatga
     6241 gtttgtgttg cataactgga cgccggctcg tttcaaagta ggacaaatga ttggttcttc
     6301 aggtatcttg atgggaatgg cttttgcgat gtatcgtaat gtcgatcctg ataagaaagc
     6361 aagatataaa tcaatgtatt tttcagcagc gttggctgtc tttctaacag gtgtaacaga
     6421 accattggag ttcatgttta tgtttgctgc tgtgccatta tatgtcattt attcagtgat
     6481 ccaaggggct gcatttgcga tggcagatat cttgccattg cgggtccatt catttgggaa
     6541 tatcgaattg ctgactcgga caccgttagc tatcaaagcg ggtctaggtg gagatcttat
     6601 caacttcgtg ttgtgtgtga tcgcttttgg tgttgttacg tatttcttag caaatttctt
     6661 gatcaaaaaa ttcaactttg ctacaccggg acgtaacgga aattacgaca atgacagtga
     6721 agaaaccgtt gtatctaaca gcggaacagg aacagcagat cagcaagtgg tccagatcat
     6781 tcatttgctg ggaggaaaag aaaatatcaa agatgtcgat gcttgtatga ctcggttacg
     6841 cgtaagtgta aatgatcgag aaaaagtagg gactgaagaa gcctggaaac gagcaggggc
     6901 gatgggattg atcgtcaaag acaatggagt ccaagcagtt tatggaccaa aagcagatgt
     6961 cttgaaatca gatatcgaag atctgctgca atcaggagca gagattccag agccagaaat
     7021 ggtttctaca ggaaatggac aagcaggtag taagcaatac ttaggtatag aacaagaact
     7081 agtttccgca gcttctggag aagtgattcc tttatctgaa gtaaaagatc cggtattctc
     7141 acaaaaaatg atgggtgacg gctttgctgt tattccaaca tcaagagaag ttgttgcgcc
     7201 gatcgctgga aaagtgacaa gtattttccc tagcaaacat gcaattgggc tggaaacgaa
     7261 agatggtatc gaagttttga tccatatggg aatcgataca gtccaaatga atcagccagc
     7321 ttttgagatt gctgtcaaag aaggccaaga agtaggaaca ggaacgaaat tagctgagat
     7381 ggatcttgat gtgatccaaa acgaagaaaa agatccaacg atcatgatcg tatttacaaa
     7441 cgataaagta gaagaagtag tgatcaagca attaggtaca gtgacagctg gtaaagtgat
     7501 cggctccatt aaaatgtaag gagaggttgt gaaaaagctg tcctgcatca agtcataaga
     7561 acagccaaac aaaaatagct cctcctattt ttcgtttggt tgggcttatt accgagaagc
     7621 agcagtttga atactgttta gtggagaggt cgtgaaaaag ctgccctgca ccaagtgata
     7681 aaaacagcca aacaaaaaat agctcctggt ccattgagct tattactgag gagttagcag
     7741 gagaacacta tttgctggag aggttgtgac aaaaattttg tcacaaccct tttgtttcga
     7801 aatgaagaca aggagaaaac caatgaaatt catgacactc aacacgcata gctggcttga
     7861 gccagaacca gagaaaaaat tgcaagaatt agcagataag atcctgttag aagattatga
     7921 agtgattgct ttacaagaaa tcaaccaatt actggaatct gaagaagtag aacctgcgaa
     7981 gctaatgaag ttctgtccgg taaagaatca agtacctatc cgtaaagata attttgccta
     8041 ccgtttagtt cagcttttaa aggaacacgg aaaagagtat ttttggagtt gggaaatgag
     8101 ccacatcggt tatgataaat atgaagaagg aaatgcactg cttacgaaaa aaacattgga
     8161 aagtcaggtc cttaccgtct cccaatttca aaagaaagaa gattatcaga cacgcaagat
     8221 cttgattggg aaaacaaaga tagatgatca ggacattttt gtctcgagct gccacttttc
     8281 ttggtggaca gataaaaaaa gtggctttta ttttgagtgg aaaaacttgg aaaactactt
     8341 tttggaaact cgcgtaccac tttttttcct aggtgatttc aataatccag tagactctca
     8401 aggttattat actgtaagag aaagttgcct tcttcttcaa gattcgtatg ttgtcgcgaa
     8461 tgaaaaagga aaagcagcta ctgtggaaaa gaaaatagat ggttgggaac aaaatacaga
     8521 aaaattgcgt atagatttta ttttcgtccc tgaaggaatg caggttaaaa aatatcaacg
     8581 gatttttgat gggatcgata gtccaatcat cagtgatcac tacggggtgg aaatcgaatt
     8641 agatgtgaac gaataaaaaa tataacatat aacataaaaa aactgattaa aataaaactc
     8701 tccaaacgat tataccgttt ggagagtttt ttgatcggat gtatttatcc cttttgccta
     8761 agcacgtaaa agcttgtatc tatgataaat aaataaacat agcaaggtaa accagccaat
     8821 caaagtaaga gaaagaatga aatagaaata gattggcgtt tgataatgaa gcgtgagctc
     8881 gttgattcct ttttgctgct tcacgattgg ggtcccgata tctgttactt cataatcagt
     8941 caatgttttt ccattttgct gtagcacagt ccgatcgtag acgataacag ggatatgaac
     9001 agttttgtcc ccagtgtttt tccacttgat tgtcaaaaga ccgcctggat gtgtttttat
     9061 aaaagttgtt tcgttttcta gtatttcttc gttataccgg tcgtatttat tgtcttttgt
     9121 ctccttatag attggcagat agtcaggtgt ggttttttgg aaaacgaaca agctggtaga
     9181 catatctttg ctgaagaatg ttttccttgc ttcttccggc gtatcgaata gataagtatg
     9241 tcgactgaca aatgtttcat tttcccactt ttccagatgc tggtataagt tctggatcgt
     9301 ctgtgctgaa aaaataagaa tgccaaatat acttaaaaga cggaaccact tttgttcaga
     9361 ccactggttc agtgaaaggg caaggtaaag tagaagcaat agcgtaaatg gcacgaaaaa
     9421 tcgaaatgga aactggatca aactaataaa aggcacattt ttttcgatca atgtcgtcca
     9481 tggaatcaag ctagtagaaa gaaaaaagaa aacaagactt gtgtacaaca cgattttttg
     9541 gaataaggtg ttcttttgtt gaaagaaaat aaaaagcaaa aaacctgata atatcaatat
     9601 aggaaaggcc ttagggtaat ggagccaata actgccatcc agatctatcg ccttttgact
     9661 catttctctg ttaacaaagg gaggaagaat cttattccca ctgtaaacaa tagctaatcc
     9721 tgcccaaata tttgctgtca gaacaaaata aagcaagacc gacttaatca gttgaagcag
     9781 tgcttttcct ttttcagtct ggttaaaaaa gtaataaaga taaaaaggga tatacatcaa
     9841 agctaacatg atcgaagaca gtaaatggat ctgtgtcata agtcccactg agacagcgac
     9901 tttgataaca gggaactttt tatgaaggac gaaatcagca atagggatca tacataaagg
     9961 ataaaaacaa gtcccccagc tagtgaatcc ttggttgata gaccagtatt gaatagaata
    10021 cgtactcatg aataaaacgg ctactggtac acttacccaa tttcgaacat tgacatatct
    10081 cagtaaaatc agcatagaaa atcctgaaat aagataaaga atgaaatttg aaagaatctg
    10141 ataatgaaac cagctacctg caagcaacac gatcaatcct tgtaaataag cgaaaaatgg
    10201 gccgtaaacg gcattcacga tcctgccact ttgctggaat ccataaaggg aaggaaaata
    10261 atggaaatcc ccatatttaa tttgttgcgc ggcatcatag aaccgattgt aatggaatgg
    10321 ggcatcagca cccaaaatta gtttatgtgt gaataaatga ggcatgagaa atgccaaagc
    10381 aatgaaaaaa attgctaaaa atgggccata ttttttcaat tgtcgaactc ctttgcaaac
    10441 tttagataaa ttcatcatat atcctatgcg agaggtaggc aagaaattag aaaattcatg
    10501 aaaatattta tgaattttac aagaaaaaca taagtgaaag ttctaaggag agaggatgcg
    10561 acataagttt tgttgcatct ccttgttttg aaacagaatt tttcttaata ctcgaaacaa
    10621 agtagctttt tcatgacctc tcggtaaacg gtgttcaatc agctgcttct cggtaataag
    10681 ctcactagcc tagaaaatat gagaagctat tttctgacta gcgctcttat tactcaaagc
    10741 ataacgcagt tttcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa
    10801 taagcccaac ccaacaaaaa atatgagtag ctattttttt gggctgtact tattacttga
    10861 tgcaggacag ctttttcaca acctcatcgg ctttcatttt ctattttttt cctgaaccca
    10921 tataataaaa gaaagaacaa aaaaacttgc aagggaggac acatgtgaag cagagtaagc
    10981 aaaaacagtc tcgtcgactt ttgtttcgtt tgtttttatt aatatgtatc gtcgtagggt
    11041 tactgatcgc tttatatcca ttttatgtgg attctttaaa tagttttatt gatcaaaaac
    11101 gtatcgaaca agtacaaaaa cggaccgcag ctgaaattga agtgcagcga aagaaaatgg
    11161 aggagaaaaa ccaacgatta actgaccaag gattcaatcc tggtactgat cctttcaatg
    11221 aaaagaacag aaatgaaagt acaacatcca gccaattaga ggaatggctg atcggaagta
    11281 tcaatatacc taagatccaa ttaaatattt cactttatga tcggctcaat ggtaggatcc
    11341 ttgaaaatgg agcaggcgtc ttgcaaggaa cctctttccc gcttggtgga aattcgacgc
    11401 attcagtgat ctctgctcac agcggtttac ctaatcgacg gttatttacc gaattagatc
    11461 gtttagaaaa cggagacacg tttattttga ctgttttggg ggagaaattg gcttatcaag
    11521 ttaaaaatat acaagtggta ctgccagatg acacgtctgt tctaacgatt tcagaaggag
    11581 aagacttagt taccttgctt acatgtacgc cgtatatgat caacacgcat cgtttgctag
    11641 tgacaggaca tcggattcct tataacgaat cggtaaaaaa agaagaagga aaaggaaatc
    11701 aggaaagaac catgcggcaa tggtttattc ttgcaggaac gataatagct gtagtcatac
    11761 tgttagtgtt tatcggacga ctaatctatc agtatagatt aagtaaaaaa gtgcttgatt
    11821 tttcatttat tattagtgat tcgtcaggga aaccggtaaa tggtagttca ttcattttga
    11881 aacataagaa aaaaacactt actagaaatg gtgtgccgtt ttcagtacaa agcgaccatc
    11941 atgggaaagt caagctggat caattgcctg gtggaactta tcgaatcgtt tcagctgatg
    12001 atcctaaagt tgctgtttct tttggtatta gaaaagtaaa acaagagaag atatactttt
    12061 ttgaggggcg aaaacttgta aaagagcttc aaaaaaatgg tttttatttt aaaatcaatg
    12121 aataagtaag gagagaaaaa atgatccgca caacaacaaa attgtctgcc aaagaactgg
    12181 aatctgttct atcgatttgg ctgcatgcta acttggattc tcatgaattc attccatcaa
    12241 cttattggga gaatcaagta gaatctgtta ggaaagcttt accagatgca gtgatttact
    12301 tagcagaaat agatcaagaa attattggtt ttgctggggt gattgatcag tttatcgcag
    12361 gaatctttgt gaaaagggaa taccggcaac aaagaatcgg tacgcaacta atgaaaaaaa
    12421 taaaagaaac caataaaaca ctgactttga cagtctatca aaaaaatcaa tcagctattc
    12481 atttctatct ttcacaaggt tttacaattc aaacacagca aaaagatgaa gcagtaaatg
    12541 aggaggaact tgtgatgaaa tggaatcaaa acgctgattt ttgatgaaca gaataacgaa
    12601 ataatgctcc cagcctaatt ttttgctggg agcattattt cattttgaag tcactaattt
    12661 aactgtgtga atatgcagtt gttacaatct ttgataaacg gtgttcaatc agctgaccct
    12721 cggtattagc tcaaaaaacg gcaaaatatg aggaactatt tttccatttg tttctctaat
    12781 actcaggtca aaacgctgat ttcacaacct cttgataaac ggtgttcaaa cggctgcttc
    12841 tcggtaataa gcccactagc ctagaaaata tgaaaagcta ttttctaact agcgttctta
    12901 ttactcaaag cataacgctg atttcacaac ctcttgataa acggtgttca atcagctgac
    12961 cctcggtatt agctcaaaaa acggcaaaac atgaggaact atttttccgt ttctttctct
    13021 aatactcagg tcagaacgct gatttcacaa cctctctttc acaaccttat tttactcgtt
    13081 tataattttc atcgaagtat tttccgctac ggatatcatc tggcattcca tcgtatggtt
    13141 cttcgctaat aacatcttca ttgtctatac ctgcatcacc catataagtg atcgtagcaa
    13201 attcaggaac aagtaatttt ggataagttt catatttttc acgagctgct tccatttctg
    13261 cgatatgttc gttttggaaa gtaacagtga tttctggatg ttcttcaacc catttaggga
    13321 aaaagatcat tccgtgaagt ttcttttcat ttggcataaa gtccaatgcc acgtctgtcc
    13381 ctgttggctc tgtccaagtg attttgaata cgccgtccgt caatttaacg atatcagctt
    13441 cttggtcttt tacccaacgg cctttgacca ttccgccatg gatacggtaa tcaacagtat
    13501 ggtcattttt ggcataccat tcatattccc aaccattgtc atacgtatag ataaagtgag
    13561 ttccaataaa atcttccaat tctttaaatt ctttcatgtt taagcatctc cttataaatt
    13621 aaaacatgta ataaactaca tgttttcgat tactcattta tgatagaatg gatttaaaga
    13681 tttgtcaagg agggaaaact gatggcaaga aaaaatacac ttcgatatat tttgttaggg
    13741 ttattaagta agaagaagat gacaggatat gaattgaatc aatcgttcaa aaatgaaata
    13801 ggagaatttt ggcaagcaaa acatagtcag atttatcctg aacttgcaaa aatggaagag
    13861 caaggaatca ttcagcatca agtagagatc acaggtgaaa aattggaaaa gaaagtttat
    13921 gaattaacag aagaagggaa ggaacaattg gcagagtgga ttcatacacc caccaatgaa
    13981 ctaccggtta atcgagacga atttgttctg aaattgtatt tcgtcaaaga cgtgaatgat
    14041 ccggcattag tagaaattat tcagcagcag cgtgaccttc atgaagaaaa acgacttcat
    14101 ctattagagc gaaaaaaaac gatctttcct actgaaaaag aaaaacatga aaattacgga
    14161 cactacctga ttttagcgca tgcgatcaat cgggaaacag aatatgcaaa ttggctaaca
    14221 gatgtattag aagaaagtaa ccgaaaaaaa taaaaaaata tccgaacagt gatggagaat
    14281 tcctttctat atgaaagaaa cgcagatcac cgttcggata ttcttatgtt caaacaggcc
    14341 ttttttcaag tatttatgta gcatctactt attaaaaagc ttggtactta ggagattagt
    14401 ggcgtttaga gttcttttaa caactgttcg tagtcagctt tttcatcgac aggaaggagc
    14461 ttgtaagtta attcgtctgt ctcgttgtca tgaatgatgt acatcttagc attctcttct
    14521 ttgaagttac cgatttgtaa aatcgtatag cgttcgtcga tcaattcgat atctttgatt
    14581 gcttttgaaa aaactttacg gacatggtgc attttctttt ggcggatttc tgccttttct
    14641 gcagcttttg acaaagtaaa tccgtcatct aaatattttt tgatcagttc tacttttatg
    14701 actgtaggta aacgatattt tctaggacaa ttcgcatcag cttcgatcga acgaatgaat
    14761 cccttttgtt cccaatacct taattgacgt ggagacacac ctacgatatc acttaattca
    14821 ctgatgccaa caaccaattg atcattttcc aacaaacgtt taagggaagc cccaaccatt
    14881 tttgtcactc cttttgtcct cttgcttccc ttaattatat ccgtattaac ttatgtgtca
    14941 agtaggttga caaatagcga atttaggact aaagtattct agtagtttca gaaaaaattc
    15001 agaaagaagg agaaatatga gtaacaacca acccgtagat atttacggaa aaccatataa
    15061 tcggacactt ttagttgttg tcttattaat tggtacattt tgtaccgttt tgaatcagac
    15121 cttattgacg acagcttttc ctacattgat gaaagctttc gatatttcag cgtctgatgt
    15181 gcaatggtta acgactggtt tcttgctagt caacgggatc atgatcccaa taacagcatg
    15241 gctgatcaat aaattcagtt caagaaattt atatctttct gccatgacga tctttttgat
    15301 cggaacgatt acttgcttta cagcacctaa ttttagtaca ttactaatcg gtcgtttgat
    15361 ccaagcttgc ggtgtgggag tatcgatgcc attattgcaa aatatcatgc tatctatttt
    15421 cccgccagaa aaaagaggat cagcaatggg gatgtcaggg atcgttattg gacttgctcc
    15481 tgctttaggc cctacattat ctggttatat cattgataac taccattggc gtgacttgtt
    15541 tggtatggta ctgccaatcg ttgttcttgt cttggtttta gcattcttcc taatgaaaag
    15601 tgttattcca ttgtcaaatc ctagcattga tattttatcc gctattttat caacgattgg
    15661 attcggtagt ttactttacg gattttctag tgtaggagat gacggctggg gaagcttgaa
    15721 agtaattggt ttcttagttg ttggtgtgat tttcatcgcc ttgttctcat ggcgtcaatt
    15781 acatttggaa catccattct tggaacttcg tgtattcaaa tcatctacat tcacgattgc
    15841 agctgtttta gctggtgtaa caaatatggc gatggtaggg gcagaaatgg tcgtaccatt
    15901 gtatattcaa aatattcgtg gagaatcagc attccattct ggcttgatgt tgttgccggg
    15961 tgcattgatc atgggggcta tgatgcctgt gacgggtgcg attttcgata aatacggcgc
    16021 gaaacgttta gccatcacag ggatgttcat tttgacagcg gcaactgcac catttgcttt
    16081 cttgacaaaa acgactccag ttttatatat cgtgatcctt tatgcgatcc gtatgtttgg
    16141 gatctccatg gtcatgatgc cggtaacgac ttctggtatg aatgcattgc caaataactt
    16201 gatcagtcat gggacagcgg taaataatac attccgtcaa atcgcaagct cgattggtac
    16261 agctattttg atcagtgtgt tgacgaatgt gacaaaagca aatatcccaa gcaaatctgt
    16321 attggataat acgccattag cttataaaga tcaggcaatc aatgccacac tgtcaggtta
    16381 ccatgccgcg tttatcgtag cgatcatctt tgggattctt ggctttatta ttgccttttt
    16441 attgaagaaa aaagaacctg ttctaatgaa agcgggggat aatgcatgat tattggagtt
    16501 ttgattgtca gcttgcttgc ttttgcttta acgaacgtgt tcgctaaaaa aacatggcaa
    16561 acctttttat ctttgatttt cggactaatc ttcgtcgctt cactaagttt gatcgtcgct
    16621 aatttgtcca atcactttgg aatggaaaaa gtaacggaaa caaaaacaga aaaaattgtt
    16681 tccagtgctg attcccaagg agcagatatg cttttgtata aagctttagg gaatggaaaa
    16741 gaaaaagtgt atctttaccg aacaaatgaa aaacaagaaa aaccaaaagc aactggtacg
    16801 gataacgaaa caaataaagt agaaaagaca gacggcgatg ctgaaaaagt gacgaaaact
    16861 acttattggg aatacaaaaa cgacatgtat aaattctggt tcaatattgc tgataacaat
    16921 cacgaatatg acagccgagt gaatacattc aaaatcccag agacatgggt agaattaagt
    16981 acggaccaag cagccaagtt agctgaatta gtgaagaaac agcaaagtac gatggaaagt
    17041 gaagcaaaag cttatgttca agacggtatg gtaaaagcaa tgactgaaaa tccaaaaatg
    17101 agcaaagcag agcaagaaca acgtacgaaa gccttagctg ctgaattcca acaacaagca
    17161 ttcgcaaaat tagtgaaaga agcaaaaggc gaataatatt ttgaacaccg tttaaggaag
    17221 aggttgtgag cctgccgttt aactctgagt cacaaggagc aatttcaaaa aacagcttct
    17281 catgtttgtt gactattgtg acttgtgacc gaggagttgg caggagaaca ccgtttattc
    17341 gaaaaaagga actgcgacaa gacttttgtc acagttcctt cttttttttg ctatgctata
    17401 ctagatgtaa aaaataggag aaataaaatg agggaaattt attcttttga atcaaaaatc
    17461 tatgcttccg aagtcggcaa gggcggggca tatattattt ttccgtatga tataagaaaa
    17521 gaattcggtg ctggaagagt taaagtccaa gcaacttttg acaatgtgcc atatgaaggc
    17581 agtatcgtga atatgggcgt gaaaaatcca gatggttcag tctgctacat ccttggacta
    17641 cgaaaagata ttcgtaaaaa catcggaaaa gaaatcggtg atattgtagc agttacagtg
    17701 aagcaaaaat aaaagaggtt gtgacaaaag acgtgtcaca acctcttgat aaacggtgtt
    17761 caaaagccag tttctcggta gtaagtcaaa tttatgcaaa aatgtggaaa cccatgttta
    17821 caaaattttt cttactactc gaaacgaagc ggctttatca caacctcttg ataaacggcg
    17881 ttcaatcagc tgtccctcgg tattagctca aaaacggcaa aatatgagaa gctatttttc
    17941 catttttttc tctcatactc aggtcataac gctgatttca caacctcttc tttcataatg
    18001 gtttttcacc atctttataa taaactggtt ctttgatatg gaaatctatt ttttccaacc
    18061 aatctaaaat cttgtaacct aactcttctg acatcggaaa agaggcgttg atttgtgcat
    18121 tggagatatg gtcaacatgg ataccggaag tgatcgtaca acttcccgga acggcagttt
    18181 tgatgatcac agctattttt tcggctaaga catcgtcttt atggaaccga ccatcgtggc
    18241 tagggaaacg tacagtttgc atctctgtat cacgagaaaa tgtagtgact gttccgatat
    18301 gtggtgtatc tccgcctgtt aaagtgatca ataagtcttt accgattact ttaacagcag
    18361 cttggatcgt gaaacctagt ttggttactt caactatcgt ttctttttcc atattaatca
    18421 ccctgccaac gttttgaatg ctcatttaca atttcaaatt gatccaaaag tttcattgta
    18481 tggtgattaa caagatcttg gatcgtttga ggctgattat aaaaagcagg aattggtggt
    18541 atgatttgca cacctagtcg tgctaattta gtcaagtttt ccaagtgaat ggcatttaat
    18601 ggcgtttcac gaggaacaat gactaacttt cgttgctcct tcattgttac atcagcagca
    18661 cgtgcaatca ggttgtcgcc taaaccaaca gagatacctg cgactgtttt catacttgcc
    18721 ggtacgatga tcattccatc ggtcaaaaat gatccgctgg cgacagcagc acctaaatct
    18781 ttgtttgaat aatggtaatc aaacaggctt tcgaattcag ttagtgataa gtcggattcc
    18841 agctttaagt tctgttttgc ccatggactg atgattccgt gtgtttctat catcggatat
    18901 tcttttagtt tcttcactag gttgatggca taaatggtgc ctgaagcgcc acttacacca
    18961 atcacgattt ttttcttccg ttgtgccaat tgttccattt tccgcactcc tttttatgat
    19021 gtcaaaacag ttgcagatca ttcattattt caaatatttt tcccaatctt ctacttcttg
    19081 gaattttgca cgtacaaaat gttctttcat atcgaaagga actgtaccgt caaagatagt
    19141 ttttgcactt atcccacgga aacgaataga gccaggatta tattctggta attctgatgg
    19201 gtccaatgga tggttcctca ttcctgggat cacgatgata tctttatctc cttggaaacg
    19261 agtgttgaaa gtccaaacta catcgttcat gtcgaaaata tcaacatctt catcgactaa
    19321 aatgactgtc ttcaattctt tgaaggctga taaagcaaga atagctgctt gacgctgaat
    19381 accttcatcc gcttcgtttt ctttacggat ttgcatgatg gtcatcaatt taccaccacc
    19441 tgcaggagga ttgtagacat ttactacttt tcctggaata gctttatcga ccaaggcaag
    19501 aatacttgct tctgtaggaa taccggccat agatacgtgt tcttcactgg gaccaatcgt
    19561 tgtttgcatg attggattgt cttttcggtg agtaactgct ttgactttca ctacttgtac
    19621 agctggatta gcatccccag tatagccagg aaattctggc attgcttttc ctgagttcgt
    19681 gttgatatct tcttgcattg tttcatttgg catgatttct gcttcaatga tgaattcagc
    19741 acgagcaata cccacttcat cgactgctat accatcaacg atttgaatag gttcgttacg
    19801 caaagctcct gcacaccata gttcattgta gccaagtgga gtggtaggtg gttcgaaagt
    19861 cgtgccaatg gcaattgctg gatctaaacc aatattgata gtgataggca ttggtttatt
    19921 caaggcttcg aattgttttt ggaagtgacc aatgtggcgt ccgccaggca tgatgtacat
    19981 accgatcgta tctttatctt ctagaaccat acgatggatc gttacatcgc tcattgtttt
    20041 ttctggatct gatcctaaga ctacacccat tgtgatgtat ggaccagcat cttcaggagt
    20101 atttgtaggt gcaggcaaaa tgttgcgaat atcgaagtct gcatcggtag ctagatgaac
    20161 gacttcttgt gctggagcgt ctgctttgtc tactttcaca ggtttgattg gattttctac
    20221 agcgtctttc aagaaacgac caagtgttcg ataatcatgg tgcaaaattt cacctacacg
    20281 tttacgacta gccattgttc cgatgttcac acgtgtgccg gggaaacctt tgatattgtt
    20341 gaaagtcata gttggtcctt cttgagtcgg acgttttact gtaccgccag caccgatata
    20401 acggtaaaca ccagataatt ctgcttcagg atcgatttca atatctgttt cgtgatattg
    20461 acctggaagt gtttttagtt cttcgatgac ttttcttaaa tcatacggtt gatctgtcat
    20521 gagagattcc tccttagttg tctttaccta tctatagtaa aaggtgaaaa agaggaaaac
    20581 aattcaactt gttcggtaaa gtattctaca atggaatgga tgcgtttttt tgataataga
    20641 ataaactatg atatacttat cactaaaaga aagcgcatac ttagtgttgg aggaaggaaa
    20701 gtgaaaaaaa tggaggagtc atttcctaaa gcagtaaaag tggaaaatat agcgaatatt
    20761 ttgaaagtta catttgaaaa cggagaagta aaatacgtga aatctcattg gacagaagag
    20821 ataaccgatg ccttgcaatt tggaaaaaaa ggaagaggaa aaagaaaaaa tcttctcgca
    20881 ctttctcgta atatgtggat tgggactgaa gtaacgattg aagcagatgg aacagtattc
    20941 ataaatggaa aagacaggta tacgccagaa gagttgtggt acaaaggaaa gaaaagtata
    21001 ccagaattat aaaaaataat taaaaaaaac agcgcgaaac aaggaacata ttacttcctc
    21061 gtttcttgct gttttctact atttcagcgc tcttatgact caaatctaaa tgtggttgtc
    21121 acaaccttat tctgcacgca aagcgatagc ggcgtctttt ttcgctgcca ttcgtgcagg
    21181 aagatgaccg ccaagcatcg tcaatatagt agagacgatc actagaataa tcgcatggac
    21241 aggatttagt tgagcaacat tttttagatc cgtcatgttg tataagatgc tattgatagg
    21301 gaacgtagct agccatgcaa taaacacacc taatatccca gaagaaatac ctaaaataca
    21361 cgtttctgcg tcaaatacgc gagtgatgtc ttttttacgt gcacctaaag cttttagtac
    21421 accgatttct tttgtccgtt caatcacaga tgtataagta atgatactga tcatgatcat
    21481 acttgttact aaagagatcc ctgcaaaagc aataaggaca taagtgatag catccatcaa
    21541 tccgccagtc agttcagtca tcgttccagc taaatcagta taaatgattt tatcttcatc
    21601 tgatttgcct ttattgtaat catctagata atccaagatc ttttctttgt cttcaaaatt
    21661 gttcggataa atcatgatac ttgaaggcaa gctgtctcca cctaaataac taagcaatgt
    21721 ttgtttggca ttttcgtcaa ctttttcagt agttaagaca tttacaccac tgtctctttg
    21781 agctttgacg atttctgatt ctttgttttc gttgacgatt tgagtcgtta attgatcgct
    21841 ataagcgatt cctggagata agagattcat tgtagaagaa gatttcacgc gtaggatccc
    21901 cgagatcttc aattcatcag aagcattctg atacatcgca tcataatctg tattagggat
    21961 aaaattccca gttggaagtt ttgtatagta agtattgttg tttactaatt tgaatgttgt
    22021 accaacgata tcatcaaaat caagtttttc gtcttctttc acatcaaatc caagattctt
    22081 caatgcgttg atattagtat tattattgcc gtctacgatc agcacgacat ctgttgctga
    22141 ggctggataa cttccggcaa gtaaagaata attgtctttt aaaaagtttt ctttgctagt
    22201 gtcaagctgt gtagggaaac ttgacactcc cacacctgtc attgctgaca tcgtactaga
    22261 taaagaaaga gattccgtat caggattttg attcgagaag ctgacaggct gtactttgcc
    22321 atttacatca cgtaaaagat tgattcctgt agtacgggta aatccgatat tattacttaa
    22381 gttaggatcg atgtctgtta cgtaatcgac gtatgtttga tcaatcttat tcgtgtgctg
    22441 tgctttatct tcttcgctga cttttgcagt aatcgttttg ctgtctggat aatccgcttt
    22501 gcttgcaccc aatccggcat cgtcacgagt ctggttagtc gttaccttag aaatagtaat
    22561 cggaaatttc gccatcgttt cagcttgcgt gttatcgatt tgtttttgga atccagaaga
    22621 aagggacaga acgatcgcga ttccgatgat cccaatactg gaagcaaagg aggtcaagaa
    22681 agtacgtcct ttttttgttc ggatattgtt gaaggaaagc tttaacgctg tccaaaaact
    22741 catctttgtt ctgcgtaagt taaattgatc atcttttggt cgttcgatgt gaggatggga
    22801 atctgttaag atcttaccat cagaaaattc aatgattcga tctgcgtatt gatgggccag
    22861 ttcaggatta tgtgtgacca tgatcactaa tttttctttc gataactctt ggatcaattc
    22921 catgatctgg atacttgttt ctgtatctaa cgctccggtt ggttcatcgc acaaaaggat
    22981 atctgggtca ttggctagtg ctcgagcgat ggccactcgc tgcatttgtc caccagataa
    23041 ctgatttggc tttttatgca tatgatttgt caagccgact cggtgcaatg catcttctgc
    23101 ccgttttcgt ttttcgtctt tagaaacacc acttaatgtc atcccaagtt caacgttttc
    23161 aataatgcct agatgaccaa tcaagttgta actttgaaaa atgaaaccga tagagttgtt
    23221 tcggtaggca tcccagtcac tgtccttaaa gtctttagta gactttccgt tgattaccat
    23281 atcaccggaa tcataattat ctagtccacc aatcacattc agcatagtcg ttttaccaga
    23341 accactagga cctaaaatcg ctacgaattc tttttgtctg aaagcgacag acacaccatc
    23401 caatgctttg gtggtggttt cgccgacttt ataatatttt tttatatctt ttaattgaag
    23461 cataatcacc cttctcccgt attgaaatat tcggtatact aataagtata tcagaacaat
    23521 gatcgtagta tacgaaataa atgttaactg aagatgaact acaagaaaaa atctagaaat
    23581 ttttcaaaaa acagagggag atcaatgaat cgtattttag tagtagaaga tgatccgcaa
    23641 ttacagttgc tttaccgttc tgtgttggaa cgagcgggct tttcagtcat caccgcattg
    23701 aatggagagg atgcgctgaa aaaactagaa gcgacacgta tagaattaat cattacagat
    23761 atcatgatgc cgaaaatgga tggctatgac ttgctagact cattacgtag caccagattt
    23821 gacacacctg ttctaatgat tacggcaaag gccgattttg aggataagaa acgcgggttc
    23881 aaattaggag cagatgatta tatgacaaaa cccatcgatg tcaatgagat gatcctacgt
    23941 gtggaagcat tgcttcgacg ttcaaagatc aattacagca gtagcttgat aatcggacaa
    24001 acaaaattag agctagatgc ttatacagtg ttacaagatc agcattcgtt gattctgcct
    24061 caaaaagaat ttctgcttct gtataaatta ctgtcttatc ccaacaagat cttcactcgc
    24121 caacagttga tggatgatat ttggggatta gatacgaata cagaagaacg aacgatcgat
    24181 gtacatatca aacgtttaag gacccgtttt gaaaagacgg aagattttca aatcatcaca
    24241 gtacggggac taggctataa ggcggttatt taaagatgaa aaaaaagcta ttttcacaat
    24301 tatggatcta ttttgctagt attgtcttca tttcgatctt agtgacactg ctgtgttttc
    24361 tcggttttat tttcttattg tctcgacatt ctatctcgcc tgctgaatcg gctggaaaac
    24421 caacgttata cccgttattc gtctttgcgg ggttcagtat ggtcgtgggt acaggaatat
    24481 cgatttttgt tgggcggcgt atcctgcatc caatcagtgc gctagggaca aatatgagtc
    24541 tggtagcaac gggagatttt tcgattcgaa tggatgaaca gcagaaagta gcagaagtac
    24601 agcaactgta taaagatttc aacgttatgg tccaagaact caatagtatc gagacactga
    24661 ggaatgactt tgtgtccagt gtatcccatg aattcaaaac accactagca acaatccaag
    24721 gatatgttca attgttacaa gccccaaatc tctctgacga agaacgccaa atatttttat
    24781 atcggattat cgaaagtatc acgcagcttt cacaattaac agagaacatc ttaaaattga
    24841 acaaactgga aaaccagcgt atccaactgg aaaagaaaga gtatcgatta gatgaacaaa
    24901 tcagagaagt aatcgttttt tttcagccta aatgggagaa agaacagcta gaattggata
    24961 tcgaacttgc tgcagtaaat tacacaggca atgaagagtt tttgtaccaa gtgtggctga
    25021 atatcatgga taacgccata aagtacaatc aaatcaatgg acagattcat atcaaactat
    25081 ttgagacagc cacagagatt gtactggaag tgaccgattc aggtgtagga atgaatgaag
    25141 agaccagaga tcgaatgttt gaaaaatttt atcaaggaga tacaagtcgt caaatttctg
    25201 ggaatgggct aggactttca ctggttaaaa aaattctaga actgcatgat ggacgtattg
    25261 attatagcag tatcgaagga gtaggaacga cagcaatgat acggttaagt aaacaatagg
    25321 aaaaagaata aaacccctaa aaattctatt gaaggttttt aggggttttt agtcgttttt
    25381 ttgtgattag ggaaatgttc ttacggttct tttggcataa aaatcgctgc tttcttattg
    25441 aactacgaga aatgttagag gtgtcattga aacaaggaca tgatgcaaga aagagagcga
    25501 aagtcatgcg tcgctctctt tttttgggtg gatagatgga cagaaaatgt agggcttagt
    25561 tttcttcttg atttaaatac tcccaaaatg tatctgaagc caaagaatgc tcaccgactt
    25621 tacgagtaaa tgcaagataa ctctctgttg ttggagaaat agggagaatc acagtatttt
    25681 cttttaatag attcttgact gttttttcca taacaatagc aatccccatg tcatttgcaa
    25741 tcatattgat gatcaagtcg attcttgctc ctttataaga gatttttggt tcaaatccag
    25801 cttcgcgaca cagatctagt acaggttgat acaagttcgt agtggggcca aggagcaaaa
    25861 agttctcctt tttcagttcg cttagatcca atttcttttt actagctaaa ggatgatttt
    25921 taggtagaac tgctacaaaa tcgtctgttt cagtaggcaa ccactcaatc gaacttggtg
    25981 gggcctggaa acttctagca aagtggatcg tgtcactatg ttcattggcg tcattgtaag
    26041 ttaattcgac actttccact tctttcaact ggactgtaaa ttcaggatgg cgtttcaaaa
    26101 actcggtgat tttggaaaaa cctttataat tgatcattgt cggtatcgtt aagatattta
    26161 atgaaagatt gttgtctttc gtataggttt caagcacttc ttgcatagca tggtattgtt
    26221 ctacgacatt ttttacatat gggagcaata agcttccagc ttcggtcaat tcgatttttc
    26281 tattggcgcg ttcgaataat ttgacgtcta attctgtttc taacgaaaga atctgtttag
    26341 aaatatttcc ttgggtggtg aattgtcgat cagcagtttt ggtgaaattc attgtttcgg
    26401 ctaaatcaag aaaaacttcg aatttatgga tatccatcat attcctccta cttattccaa
    26461 taaagaatac tttaacacaa aaaatgaatt ggtgtaatct tctctgtctg ttacaatctt
    26521 tcttgcagcg aaagcaataa ttacagaaat ttttctgatt tttttcgctt cgtagaaaaa
    26581 gagaggagtt ttttgatgcc aactggagtt ttgatcaatg taggttccgt tttgtttggc
    26641 ggactaattg gcggattagt aggaaataaa ttaagtgaac attttaaagc acagctgacg
    26701 atggtttttg gtgtgtgttc gatggggatg ggaatctatt ccattgcgcc gatgaaaaat
    26761 atgccggctg ttatttttgc tttagtgata ggaacagcga ttggtttgat tgttcattta
    26821 ggtaatggga taaataaggg ggctgcgctg atgcaagtac ctatttctaa aatcttccct
    26881 agtgaaaagc ttggtatgac ccacgatgaa tttatcagca cattagtgac ggtcattgtt
    26941 ctattttgtg caagtggtac ggggatctat ggaagtttag attctgggat gacaggagac
    27001 agtacgattt tgatttctaa atctgtcctt gattttttca cagcagctat ttttgcgtgt
    27061 aatttagggt atgtcgtttc tgtgatagca attccccaat tcattatttt ttatattttg
    27121 ttcttattgg caaaatttat ttatccattg actacaccag acatgatctt agattttaaa
    27181 gcttgcggcg gattcttgat ggtcgctacc ggttttagaa tgatcaacgt gaaaatgttc
    27241 ccagtagctg acatgatacc tgcaatgatc gttatcatgc cactgagctg gatgtggaca
    27301 aattggatta tgccactgtt ataataaaaa tcaaagagaa gccatttttt gctgactaaa
    27361 aaggatcaaa tcttttagtc agtttttttg tatttgacaa actgattttg aaactatatt
    27421 atatgttgta taatatagat tgaaataaaa tattacactt gagaatagaa ggtgacgtaa
    27481 tgcacatcca gattccaact gttcttcttg atggtactgt gctagcagca ttaagcaagg
    27541 aagatatgta tgggtatgcc ttaacaaaaa tcgtacaaga aagtctctct gtcagtgaat
    27601 ctacactata tccggttttg agaagattga aaaaagaagg atatcttgag acctatgatg
    27661 aaccttttga aggcagaaac cgtcgatatt actcgttgac accaatggga agtcgtcatt
    27721 tagaagaaat tattgatgaa tggattgttt ttcgagattc tttagaccgg ttattggagg
    27781 agagcacaaa tgaatgaata tttgaatgaa gttgtggatc atcttcaaga aattcctgaa
    27841 gacgatcgtt ttgatttgat ccagtattat gaggaatatt ttttagattc tggaaaaaca
    27901 ttagatcaga tcattgatga atatggtaca ccaaagcagt ttgccttaaa attaaaaatc
    27961 agttactttt cagaagaaga acaagcagaa aacgagttag tgaaaaatcc aaaaagacaa
    28021 attcgcctga tttggttaat cattatcggt ttatttgctt ctcctgtcct cattccacta
    28081 gcactggggt ttatattggt tattggggga ctattggttg ctttactagc gctcattttt
    28141 agtatctatg ttttatgtat cagtatctta ggtgttggcc tattcatgat catctcagga
    28201 atcagtgtgt ttggacaatc gattctgagt ggattattct ttatcggtgt cggtctgttc
    28261 gccacaggaa gcgcagtatt tatttcgcca ctgttgctga aaggaacaaa agggcttttt
    28321 tatgtcatga tgaactttgt taaatggatg ggacgacgtt ttattacaaa aagagatctg
    28381 cattcagcgg gcgtttgaag gaggacacat aaattgagat tgaaacattt tttgattacc
    28441 ggtattggtc tgatgcttac tggtggcctc ctagcaggtg caacttatac attaggcgct
    28501 caaaagagtc tgacttggga taatggtcct cggttgatcg atatggttac tgagacaaaa
    28561 aaaatcaagg atgcaaacaa aatcgtcatc catgcagaaa atcaaacagt ttcggtcata
    28621 cgcggattgg attttgagat aaaaacgact tatgataaga cagaaaagcc agaaataaag
    28681 gagaaaaatg gcgtgttgac tgtgaatgca gtcaaaaaat caacaggtgc aatcattagt
    28741 ttagaagaca caagtgcaca aatcactatt gttgtccctg cagatcatga attatctgaa
    28801 ttgacaatcg aagggaataa tagtgcaatt gatatcaacg aagtatcaat caaaaaagtg
    28861 aatactaaga cacatgatgg ctgggtatcg ttcaatgaat ttacaggagg aacaataact
    28921 gcgaaagatg aattaggctc acttcgatta gatgaggcca atgtggataa cttgatcgtc
    28981 acaggtgaaa acagcaactt ccatctagaa gacagtttga tcagcaaaga aggaaaagtc
    29041 atcctaaacg gtggcaattt atctttgcat gagatggaaa acagtggcta tactatccaa
    29101 acgtctggta aatcttatat tgaaaaagac taccagccaa taacaaatac tttgttagaa
    29161 caaggacaga aaaagctaaa gatcactgct aaagacacat cgatcaatat tacgacggat
    29221 caagaggaca atgaaacaga ttgatcctgt actcaactgg atattttaag aaaaaacagc
    29281 aaagatcctt ccaatctttg ctgtttttca tcgcttaacg cacataacta tagtaaaata
    29341 agaaagaaaa tatctaaaaa gaaaacaggg tgaaagagta tatgaaggta gaagaaggtc
    29401 aaagagaaaa gttaaaaaca gaaatggaaa gattacatac ttatatcact caactaagcc
    29461 aaaccttcta tgatccagac aaagaaaaag tgatggttaa ttacccgaac aattcagaag
    29521 gtcgccaatt agagcaagtg taccatgaag ttttcaaaca tttattgacg gtgaagaaag
    29581 agctggatta ttattcttta ccgattatcg atacaggaat attgaagtat gatgggaaga
    29641 aggaacgatt tatctttaaa tctgttcggg aagatttgcc tctttcagca gggatggatc
    29701 tagaggtctt ggtagaagac tattttacag aagaaaagca ctgggttcga acaaaattgg
    29761 attatttacc gcaggcagcc ggagggacac aggcaagcgg ttggtacatc actgaagaca
    29821 aagagttaga actagaaggt gtaatggcgc gtattcgaaa aaaaaattaa aaaagtaaca
    29881 ttagatattt attgaaggat aaaggagcgc aggaagtagc atgagagaaa aaagagcatt
    29941 ttatatcaat ggttatgcag ggattgtcgg attggtcgta ttagctttag ttggattgtt
    30001 tttcttctat ttaggtatgt ggcaagcgaa agtttgggca ctttttttaa gtatttttct
    30061 ttggttgatc acgttattgt tgttaagttc ggcaactgta gtcagtccta atcaagcaaa
    30121 agtaattttg ttttttggac aatatttagg tacgattcga gaaaatggct ttttccttac
    30181 gatacctttg gcacaaaaaa tgactgtttc gttgaaagtt cggaatttca atagttctgt
    30241 tttaaaagtc aatgatttgg atggaaaccc catcgaaatc tcagcagtag tagtctttaa
    30301 agtcgttgat acagcaaagg cattattcga tgtagcgtat tatcaagatt ttgtagaaat
    30361 ccaaagtgag acagccattc ggcatatcgc tagccaatac ccgtatgata cattcaatga
    30421 gaatgatttg acgcttcgag gaaacacgac ggcagtgtct gacgagttgc aaaaagagct
    30481 gcaagagcgt ttagcagtag caggtgtcga agtaatcgaa acacgcttga accatttagc
    30541 ctatgcaaca gaaatcgcta gtgccatgct ccaacgtcaa caagcaaaag ccattttgtc
    30601 tgctcgacaa accattgttg aaggagctgt cacaatcact caaatggcct tagagcaaat
    30661 cgaagatgga caagaaatca atttcaccga tgatcggaaa gtccaattga tcaataattt
    30721 acttgtctca atcattacag acaaaggaac gcaacctgtt atcaatacag gcgacatgaa
    30781 agaccgtaag tgacatactt tttgtaagat aggaagaagg agtttaggac ggattattgt
    30841 cttgaactct ttttttagtt gttttaaaga aaatgacaaa attgaaagaa gaatgatagt
    30901 tgattcgaag gcagaattgt tcgctttttt ctataatcag tatataaagt aagagagaag
    30961 gggaaaaagt gaaaaaactt ctgatcggtc tacttgtttt catcgttgtg gtacttgcag
    31021 gggtgaaaat cgcagattac gtagttatgg gtggagaaga atactatgta caaattacga
    31081 cagatggtac aaaaacagtt tcaaaatcag actctggaca agaaacagtc aactatagat
    31141 atatcctgcc tggctatacg aaagagggta aggaaaaaga actggacttc aagggacaat
    31201 tgactcgacc gcttcgtaaa ggagcatatt taaaaatcac ctggaacaaa aataaaggtg
    31261 tcacttcgta tgaagaagtc gaaaaaaaag acgtgccgaa aacagcactg gaaaaactaa
    31321 ttaaagggta gggaaagaaa tgaacaaaat agtcgaagta cagaatgtaa caaaagtata
    31381 cggaaaaaac aatgagaaaa agacgcaagc attaagcggc atcagcttcg atgtagaaaa
    31441 aggcgaattt attgggatca tgggcgcttc tggttctggt aagtcgacac ttttgaatat
    31501 tttatccaca ttagataaac caacagatgg gcatatccga atcaatcaaa aagatgtgac
    31561 cacattaaaa ggaaatcaac tagcagattt tcgtgcgaat gagattggct ttattttcca
    31621 agatttcaat ttattagaaa gcttgacagc acaagaaaat atcgctgttc ctctttcttt
    31681 acaaggcgtc agaccaaaag aaatcaagca acgtgtccaa aaaattgcag aacgtttatc
    31741 catctctcat attttggaaa gctatccttc tgagatttcc ggtggacaaa aacaacgggt
    31801 cgccgccgca cgtgcattga tcacacaacc aacgattcta ttaggggatg agcctacagg
    31861 tgcgctggat tccaaaagtg cccgggatct tttagatacg atggacgaat tgaatacaaa
    31921 agatcatgta tcgattcttc ttgtgacgca tgatccgttt tctgcaagtt attgccaacg
    31981 tattttattc atcaaggatg gcggtatcca tcaagaagtg aaacgtggag atcaaagccg
    32041 tgattccttc tatcgagaaa tactatctat tcttggaaac ttggaacagt agggggagat
    32101 tgactatgct ttggaaatta tcacttactg ggatcaagag ccgtttaaga gactacttgg
    32161 tgttattctc aggattagtt atggcatctg ctatttttta catgtttgaa tcaatggcaa
    32221 gcaacgaagc ttttttgaaa agtaatacga tcgttgcggc tacagttatc attttccgat
    32281 tcggttctgt tttactaggg atcatcacat tcgtttatat tttatatgca aattcctttt
    32341 tgatgacgat gcggcaaaaa gactatgcaa tgtttatgat gctgggagca aaaggcagaa
    32401 aaatcgcaca gatgattttt tctgaaacat tcttagtagg gatcgcagct actttagtgg
    32461 gttcagctgt cggtgtagga ttgacaagta tcgtccaccg attattagtg gatcagttag
    32521 atctcaatat ttcgcatttt acacctttta gtattcacgg gttgttgatc actgttttgt
    32581 tttttgctgt tttgttttta ttagctgctg ttgtcaatgc tttttcgatt gtcaaaaaac
    32641 cgattttagc attgattcgt gcggatcaga caccaacaag aatcaagcag cataaattct
    32701 tgtttttcct tgaagtagta ctgggaattg cttttctagg ctgcggttac tatatgatga
    32761 aacacgtggg gacctatcaa gtctttggta taggggtagc cttagttacg atcgtattag
    32821 gaacttattt cattttccat tctgtgatca ttttcttttt atcattacta aagaaatcag
    32881 aacagatttc attgaaaaaa ttgaacaatt tcacattgtc acagttaaac tttcgtattc
    32941 gtgaatatac acaaatgctc tcaatggtcg ctatgctgtt tgccttggca ctgggcgctt
    33001 tgacggtagg attaggtttt aaaaatgaaa ttgcagactt gacagaaaac aattctggat
    33061 atgatctagt gatgaataat cctcgggcag aagatcagca aaaaatcaaa gaactttcac
    33121 caacactttc tgcttcttat actcaaaaag aagatgcggg aacgatttac tataatgcaa
    33181 gtgaatttgc ggctcaacca ttgatcatga taaaacatga agatggaaca ccgccgaaga
    33241 tcacgaaaga aaaagttcct gctaaaaaaa tgaatgaact gactgtacaa gaggaacttc
    33301 gaagctatga attgccggaa cagcaagaaa aagaaatcaa ggtagtcagt caatcagaat
    33361 ttgatcaact gaacttgcca caatccactt tacagctagt tcaggtaaaa gacttccaag
    33421 caaacctgaa accaatcgaa acacttgttg ctcaaaataa aatgaacaat ccatctttgc
    33481 aaaaagtaga gtacagcaac cagaaatatg aaatgtatga agtgtataat ggtctattct
    33541 ctggttttga atttatggga ttcttcctag gtttagcttt cttgaccatg cttgctagct
    33601 gtttgatgtt caagatcttg tcaggaagca agagtgacat tgctcgttat gaaatgcttg
    33661 aaaagatagg aacacgacgc gggttgctta aacaatcgat ccgtagagaa gtaggcgtgt
    33721 tattcttagc accaggaatc ttaggtgtag tccatgtctt attcggatta caaatgtttg
    33781 gtctattcat gcaaaatcca tacaaagata tttgggttcc gtttacgata ttcttcgtat
    33841 tgtatttcat ctactatgta ctgacgacat ggttatatac tggaatcgta ttgaaaaaga
    33901 gattgtgaaa aagctgtcct gcatcaagta ataagaacag ccaagcaaaa atagctcctc
    33961 atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttgttc
    34021 ggaaaaagag gttgtgacaa tatttttgtc acaacctttt tttcacggtc ttttattaga
    34081 atatagtaaa ttttttctcg ttactcacgg aataagctgt taagaaaacg gtttccatgt
    34141 atgctggaat cagtgcaaat gaaagaaaag agggtgcaag gaacatggaa atcaatgaac
    34201 aaaattttgg cgaaaactat cacttgatta cgatcacgaa tcaaaatggg acatctttgt
    34261 ctgtcagtga tcttggggca cgggttgttc attttcaaac aataatcgat aatgaaaaac
    34321 gagagttgat cttaggtttt gatgaagcaa aagaatatct agaaaaagac ccttatatcg
    34381 gtgcttcaat cggacgtatg gctggacgga ttacgaatgg cacattcaaa ttagatgatg
    34441 aaacgttcga agtaaaaaca gacccggaca ctggtcattg gtttacatgg tggtgcacct
    34501 agctttgaag ccaagcagtg gcattataca gtcatcaatg gtgaaaacga agcatcggtc
    34561 attttctatc agactagccc agatatggaa aacggatttc ctggaacttt agatgtagaa
    34621 gtacggtata cgctgacgaa cgatgataca tggcgagtga cggtccaagg gatcagtgac
    34681 cgcaaaacag tattcaatcc tacaaatcat gtctatttca atttaaccgg tgatccagca
    34741 cagtcaatcg acgaacatat actttggttg aatagccgat tatttgctac gctcaataaa
    34801 gatacaacgc ctacaggaga agtagtttca gtaagtggca cagcatttga cttccaacgt
    34861 cctaaaaaat taggtgaggt ctttgccagt gattttcgcc aatcaatgat agtgggcggc
    34921 atcgatcatc catttttgtt gaaaaaacaa tctggtttac taaatgctgc ggaattgaca
    34981 agcccagatg aaaaagtatc gatccaagta aaaacagatg cttcaggaat cgtgattttt
    35041 acagcaaatt ttggagaaga tggaccggaa atgcgcggga ataaattagt aaatcatggc
    35101 ggtatcacat ttgagactca agaactgcca ggagcggagc gacttgaaag ttttggagat
    35161 gtcactctgg aacctaacca agtacgcgag tttgtaacgg aatacaaaat aaatataaag
    35221 aaatagaata aattaaaaag ggagtgtgac agtgtcacat ccccttttta atttattcta
    35281 tttctttaaa ggcagatcaa gccagaatga tttgtctaaa cttaacaagg ttagtacagg
    35341 aacagttgca gctttaactg tacgagtagt tgtttctggt atctgtcttg ccgtaaaatt
    35401 gttccacaaa tcccagattg aagcgacacg gaagatggaa gaagcgttgt tgacgcgttt
    35461 tatcgttgaa aattctaatc ctgtgattgg cacgccatct gttgtccatt tgattggcac
    35521 atcgaatcgc ttagacatat tgaggatttc agcgtttgct tcttcagttg ttgctgcttc
    35581 ttttttcggt tcatcatatt ttgtcaggtc gtacgcttcg atgatcgcgt tcaatttttg
    35641 tgaatattgg cgatctgtag cataacgtcc ttccaaatat ttggttgctt ctttgtagtc
    35701 ttttgtttcc gacttccaag ttggtttgta gaaatcagga ttgccagtca gtccattttt
    35761 gatcaagtcg gcatagtctt ccaaactttc cttgtaagaa ggatatttac ggaattgtgc
    35821 gcggatttgg tagctttgtc cggaactatc ttgttcaagt gtattgaagg tcacgctgct
    35881 tcctttatat gatcctttga taccaaaaag gttatgattt ggttcgcttg ataataagct
    35941 gtttccagat ccactttcaa ggatagcttg agccatcatg actgatgcgt agattccttc
    36001 tttttgcccg atttcctgtg catcatctgc aatctttttc aagaactgat cagttgtttg
    36061 gttttgactg aaatggaacg agccatctaa tccatctaaa atagatgagc cgcctgtcag
    36121 cgaacctgaa gcagcaggta aacctgctaa gtttactcgg acagcaaaat caggcgtgaa
    36181 atctgaaata gaagttactc gaactttatc tcctggttga ggcgcatgga taaattgtcc
    36241 gttcccaata tagataccta catggtgagt aacaccggaa gaaggtcccc agaagacgag
    36301 gtcgccaggt tgggcctgac ttactggaat ctgcgtacct gcatattgct gttcgcctgt
    36361 ccagccaccg atataatgac cggttgcttt catatatacg taataagtca aaccagaaca
    36421 gtcaaaagta tttggacctt ttgctcccca tacatatggt tttccaagat gcttgaatgc
    36481 ttctcgaacg attgcttgtt ggacattcgt tgtatttgta ggcaatacag catcaattgg
    36541 tgattttaca acaaaatcag aagaagcaga agatggattc tctataccgc tttgatttgc
    36601 tgtatttgga cgagatgaag cattgttcgt tccgccattc gatgaaccat tgtttggatg
    36661 gtttggttct ctctgagtat tttcattgtt attcgatcca gatgggtttg tttgattcga
    36721 agaattcccg ctgttcgaat tattgttatt attgtttgaa ttatttgaac cattattatt
    36781 gttgttatta cccacatctg gtttgtttgg cgtttccgga tctggtgtac tagggacttc
    36841 ttcactagga tcagttggtt tctctgttga agtatccggt ccagctttaa attctgcagt
    36901 aatagataaa ttgttcgtac ctactgtaaa ggtctttgca tcataattta cttctttggc
    36961 accattaaca gcgactgaag caatttgttc accttcagct ggcgtaatag tgtaagttac
    37021 cttcgtttcc gctaataaac cttctaccgt tccactttcc gtaatatcca aaggttgctg
    37081 ttcaggggaa tccgctgtcg aagcagtcag ttggatctta ccaatcaaag aaggagcaat
    37141 tgtaagggaa tatgtctcgg ctggcttttc attttctgat ggatcagccg gatcaatttc
    37201 agttggctcg gtcgatcctg ttgttgagtt agttgttcca gttgggtctt ctgtaaaaga
    37261 atccgttgtt tcactagtag ctgagctgga tgtagtcgta tctgttgtga ctgtttgatc
    37321 agctatttga ttatctacgg caaatacggt attggtcgac aaaacagacg gagcaatcag
    37381 ccaagtagca gctagcaaac gtatcgtttt tttatttttc ataataaact agtccttttc
    37441 ctttcattga ttttctgtct tattcagcag tcattagaca ttttttggaa cagataatcg
    37501 tgtcgaaaag ctttttcttt tcataaatct gtctgtgtat tctaaataaa acaatttgta
    37561 aataagcaac gagcattcat tcgtttttct ttgacgttca ttttattgca tagaccacct
    37621 ttgtttgggg ataaacaagt ggggataaaa atggttatgc atatgtaaat tataccaaag
    37681 tttggaaagt atcacaaaaa tttataaaaa taatgatcct aaaaagaaaa agactgaaat
    37741 atgtgataaa catctcatac ttcagtcttt ttcattaact attcataaga cattaggtct
    37801 tcatcaaacc cgctaacgag agtagtgatc attgctacga ctagactaac agccagtcct
    37861 gctaagtacc agaaaaaagt accggaaatg aatgcaggca atgtggtcag actaccaaat
    37921 acaaaagcga tcgctttcac ttgggcaaat cccattacag ctccaccagc agctgcacca
    37981 atgcaagcag caacaaatgg ttttttcaat gggatcgcaa ttccgtaaac agttggttcg
    38041 gtcactcccg caagtaaaat cgaagcaaaa gcagaaccgg agatcgaacg ggtttttgaa
    38101 cgtttacttc gcagaaatac acctaaagct gcaccagcca ttccaaaatt ggctgctcct
    38161 gtcaacggaa cgattcggtc gaacccataa gcagagatat tactgatcat gacagggttt
    38221 acagcccatt ggataccaaa tattaccaaa atattccaac caccagcgat gattgcgcca
    38281 ctgatcggtc cgttaatatc catcatccaa gtaattgcgg cagctaaacc ttcgccaagg
    38341 taaacacccg caggcccgaa taccattaac gtcaaaggaa ccgtgataac taatgaaagt
    38401 aaaggtacaa atactaattg catattttcg cgaatatatt ttttgaggaa tttttcaaca
    38461 aaagaataaa agaaaatacc taaaatcgcc ggcatgacag tagacgtata ctgcatcagt
    38521 actacaggaa tccccagaaa atcagtagct gctttgccac tgtctgaaag taaagaggtc
    38581 agattcggtt caatcagtgc tgcagcaatg acagccgcaa tatatccatt cactttaaac
    38641 gttttggcag cagaaaaagc aagaaagatt ggcagaaaat aaaagacggc attcgacgca
    38701 gcagacaaaa tatgataagt tccgcttgtt tcactaagcc agccaatcgt agtcgctaaa
    38761 acaaccagac cttttaatat accagaaccg gcaaacagcg gaatgatcgg cgtaaagatt
    38821 tccgatatca aaccaaatag tttttcgatc ggatttagct tttctttcgg agctgaatga
    38881 tcgatttgac gcgtcaattc cttgtaagca ggagccaatc cctgatccat gattatttct
    38941 gcatatctat cgagtaaaat gaaatactta atggaaggga tttgctgttt taatttttct
    39001 gcattggcta ctggctgttt tttcaatttg atacgaagcc gagtcgcaca tggaaggaca
    39061 gaatcaatat tgtcttttcc tcccaactgt tcaatcagca tatgaatctg ctcggacagt
    39121 accatctgtt caggattcat tgtcgtttcc attagaagac cactccactc actaatataa
    39181 tattggcgta aggtgtcatt tcgcctgtac gaataaatgc atgacagtta ttcaactctt
    39241 ttttcatttc ttcatgtggg ataaagctaa tcggtgtatc tggtaacaat aatttgattt
    39301 tttccaatat ttccgggttc attgtcttta tttcttccgc caattcaatc gattgtacct
    39361 caagttcttc taaaacgttt tctaaaacag ataaaaaact tggaagaccg tttgagacag
    39421 ccaaatctat tttttcagta ccaaagggga caggcatgcc tgcatccccg atacttaatt
    39481 tatcaaagtg ccccatttgt gcgattactc gtgaaatatc tgaattgatt acttttcctt
    39541 ttttcatgtt tgtttcttcc tctcattgtt aattttagac tgctgattgt tctaaagctt
    39601 cgcggtaagg gatagaaggt tgcgcaccat aagactgcac agcaacagaa gaggctagat
    39661 ttccataaag gatcgcttct ttcaagttac taaggtctgg ctgaagtgtg ctgcttaatg
    39721 cgccaataaa tgtatcacct gcggctgttg tgtcaacggc atccacttta aaagcaggga
    39781 cgataccgtg ttctttttct gtatgataga aagcgccggc actgcctagt gtaatgatta
    39841 ctgttccaat acctaattca tggagttttt cggctgctgc gaccaaactg ttatgatctg
    39901 tcactctgat tcctgtaatg atttctgttt ctgtttcatt tggtataatg atatctgtct
    39961 tttcaagtag ttcagtagga atcgtttcac gagctggtgc aggattcagg atcgttgttt
    40021 ttcctgcgtc ttttgcaata gaaaaagcta ggatcgtcgc atctaagcta ctttcaaact
    40081 gagcaataat aaagtcactg tttgtaatca gttgcttgtt ttttaggact tcttgttcat
    40141 ggaaagcatt atttgcccct gcatggatca agatactatt ttctcctgct gcgtctacca
    40201 taatcattgc ttgtccggtt gtggcgcctt ggatttcagc tatcccagat gtatcgatgt
    40261 tttcttttgt cagtagatct aataactgtt gtccttcgct gtcagcccca acgcctccaa
    40321 taaaagatgt ccgtgcgcct gaacgtttgg ctgccactgc ttggttggca cctttacctc
    40381 cgccagcgtg aaaaatctca tgcgcatgca tcgtttcgcc tggcttgggc atgttggtta
    40441 aacgaagggt agtgtccata ttaatacttc caattactgt aaccgtgttc attttacaaa
    40501 cctccaacta tctcatttat tttgatggga aaattatttt ttaaaacgtt ttagtaaaac
    40561 gttttaatag ttcgataaaa atatagcaca ccttgaaagc tatttcaagg gtgcagttga
    40621 aaatcttttt tctaatcgta caggtaatct tttttcttcc cattcttttt ctgggaattg
    40681 gatccgatct aacaataact tggcagatgt ttgtcccaat tcgaagattg gttgtgcgat
    40741 cgttgtcagt ttcggcttga tatactcgca catatctata ttatcatagc caataatgct
    40801 gtagtcttca gggatgcttt ttccagcttc ctctaatccg cgatataatc caaaagctaa
    40861 ttcatcattt aaagcaaaca caccagttgc agcagaatca agaagttctg ctgtgatctg
    40921 gtagccacct tgttttgaga actgtgtagg taagaggatc agttggtcgt gagggatttg
    40981 gtaaacatca agagcagatt taaacccctc aatccgagcg tggacatttt ccggtggatt
    41041 ttcaggatag acaagggcga tcgtttgatg tcctagacta agcaagtgca tgcccgctag
    41101 gtaaccaccg cgaaaatcgt ctgttcgtac ggcatcacta aatccttcag attttttctg
    41161 atccaaaaca atgaagggcc ggccttgttt tttcaaattt tcgttgattg catctgtaga
    41221 gacagcagaa gtcgcgataa tgaatccatc cacacctcga cgtgtgagct cggctagata
    41281 ttcgatttct ttttgatgat cagaatcagc attacataat atcgtgacga aattctgctt
    41341 atacaagata tcctcaattc ctcgcatcag tgtactgaag aatggattcg tgatatcagg
    41401 aacgagtaca ccaattgttt tcgtttcctt ggtaatcatt tgtctagcaa aatagtcagg
    41461 ctgatagcct aattcttctt tggcagctaa aactttctga acagttttcg gactgaattt
    41521 ctccgtattt ccatttaata tcaaggagac agttgtgatt gaaacgccgg aaagtttagc
    41581 gacttctttg attgtgatct tttttttcac catgttcata ctcctatcta aatcctgtgg
    41641 ttattttccc atgaaatcaa aaagaagtaa atgaaaatca catgtgccag tacttatttg
    41701 ctttgttgga tagcttcgat tatttgtttc gttgagcgta ctcgtgcagt tttcggaaat
    41761 atctttttta tgaacaattg atgggattct ttgtcacgat cactgcaagc atcttcagca
    41821 agcacgatat ggtacccgtg ttgaaaggca tctaatgcag tagcatatac accgtttgtt
    41881 gtcgctacac cgcttaaaat gatagtttcg attccacgcc ggcgaagttg aagatcaaga
    41941 gaagtaccaa aaaaagcact tggattgtat tttgtgatag tcaacatatt atctgtatct
    42001 ttcaaagaat ccttcaaaag taaatcagtg aattctggag gtacagaagc agtcattcca
    42061 cccatatcag tttgttgact caaataaggg aaagtggaga tgtctacgtt cactaaaact
    42121 ttcaaggcag cggtacgttt caatgttctt actaacaaat cattgtttgt cagaatagat
    42181 gctgcagtgt gtggagagag tgtaccgtta tttacgatct ctttttgcat gtcgatcgcg
    42241 acaaaagcag tttttgataa atcaaaaatc atattttcct ttaaatcatt catgatgttc
    42301 cttctttcaa ataacaatgt tttttatgta agtaaatcat aaatcgttta tttgtcaact
    42361 aaattaaagg attcctttct ttagtctaga gaaattcagt ggtaaaataa ataagtgtga
    42421 gaaaacgcat tcgatgcata gatatagcga gaaatatcgt gaaaatggag gaatcagagt
    42481 gaagtatgtc gaagtaaaaa atgaatacaa acgatacaaa atgggagaaa cgaccattac
    42541 agcaaatgac gatatttctt tttcaattga aaaaggcgaa ttagtgatta tacttggtcc
    42601 aagtggagca ggaaagtcaa cgattcttaa tattttaggt gggatggata cacctgatga
    42661 aggtcagatc attattgatg acacagatat tgcgcaattt tcagataaac agcttactgc
    42721 ttaccgacga acagatgtag ggttcgtttt ccaattctac aatctcgttc ctaatctaac
    42781 agcaaaagaa aatgtggaat tggcaacgga agtttctcct gatgcgcttg atcctgtaga
    42841 ggtattgagg caagtgggcc tagctcatcg attgaataac tttccgtccc aattatcagg
    42901 aggcgaacag cagagggttt caattgctcg tgcactagct aaaaatccca aacttctttt
    42961 atgtgatgaa ccgacaggcg cgctggattt tgaaacgggc aagcaagtat tgaaattatt
    43021 gcaaaacgct agcagacaac acggcaatac cgttttgatc attacacata attctgcttt
    43081 agcgcccatc gctgatagag tcattcacat taatgatgct aaagtaaggt ctgttgaatt
    43141 aaatgatcat ccttcatcca ttgatgagat tgtatggtag ggggatttta tgaaaacaag
    43201 aacatatctt aaagcaggtt ttcgcgaaat tcggcaatct aaaggacgat tcattgcaat
    43261 catccttatt gtcatgctag gaacactttt atttgtaggt gtcaaaactg ctggacctgt
    43321 catgcaaaaa acgatggatc actatgttgg tacagcagga ctttctgatt tgcaaatcgt
    43381 ttctactggt ggcttgacac aaaaagatat tgctcaagca gaaaagatcc cagatgcaaa
    43441 agtagaaaca ggaaaacaga tctattattc caatactggc aaaaacgaag tgattcaaat
    43501 attttcttat aacaaaaata acaagcagaa caagctacaa ttaactgatg gaaaactccc
    43561 ggaaaaacca aaccagcttg tattggatga aaaagcggaa gatgaagggt ataaaatcgg
    43621 tgatatttat cagattgatt cagatgaatt gaaagaaaaa gagtatacca tcacgggctt
    43681 tgtccgttct ccattgttca tcaacaattt ggagagagga tacgcgaacg tcggaaatgg
    43741 tagcgtcgat tattttgtct atttacctga atccgttttt aaaacagata tttattctgt
    43801 cttatatctt gattttacaa acgtaaagaa tttgaatacg tacagcaaag cttacaaaga
    43861 taaaatggag caaaatcaag aaaaggcaga aaagtattta caagatcgac ccgaagaacg
    43921 cttagaagag ctgaaaaaat cggccaacga agaattagaa ccggcacagc aaaaaatagc
    43981 ggatggcaaa gcacaaacaa cgcaagcaag aacacggtta gaagatgcta aaaaacagtt
    44041 agcgcaacag tcagcaacta tccagcagct tcctgaagcg caacgaacaa tcgctgttca
    44101 aacattatct caacaagaag ctcaacttgc tgaacaagaa aggcaattag ttgagaaaga
    44161 acgtgaactg gcctctgcag aggaagaatt gaatcgaaat gcagaaaaaa ttgaaaattt
    44221 aactaaacca tcatacctat acaatgaacg ttcagaaaat gtcggttttc aagaatttgg
    44281 tgatctgtct gatcgtatcg ctgcgattgc caatgttttt cctgttttct ttttctttat
    44341 tgccgcattg atcaccttta cgacaatgac acgtatggta gaagaaaacc gcagggaaat
    44401 cgggacatta aaggcgttag gttatacgaa agttgaaatc gcaaaaaaat atgcaatcta
    44461 tgcaagtcta gcctcaggga taggtattgt tttaggaacg atattaggaa cgaatctttt
    44521 gccaaggatc atttttgagc tgtctaacga gcggtatgat attgggagtg cgcttatttt
    44581 ttatgactgg ccgccgattt tacaagcagc tgccgcattc ttcattgccg catttggcgc
    44641 tgcaatgatc gttttattca aagatttaag agaacggcct gctgcattgc ttcaaccgaa
    44701 agcaccgaag ccagggaaac gcatttttct tgaatacatc actccgctgt ggtcacgcct
    44761 tagctttaat caaaaaatca gttaccgaaa tctcttccga tataagtcaa gaatgcttat
    44821 ggcgattatc ggaattgctg gatgtgccgg gctgatggtt gcaggcgtgg gattgaaaga
    44881 ttctctaagt tctgtttcag ccaaacaatt tggaacgatc attgactatc aagcaatcgt
    44941 tacattggat tcatccacgg aagatagtcg tcaaaaagca gcggatatgc tgcagaagca
    45001 agaaaaagta aatgagatcc tagctattca tactcaaacc gtagaattga gaaaaaaagg
    45061 acatgcttcg caaagtctga cagtgatcgt accaagtaaa acagctgatt tggatcgttt
    45121 tatccattta actgatcttg aagagaaccc acgctccttg cctaaaacag gaatcgctat
    45181 tacgcaaaaa gtagcagaac tatttgactt atctacagga gatcatattt cgctttatga
    45241 tgaagatcaa aaagaatgga aagtcgaagt aaaagaaatc gtgaaaaact atctaggtag
    45301 ctttatttat atgtcccctg aatattatgc ttcagttact gatcaatcga tgatggataa
    45361 tgcatttttg ctgcgtacag ataagatgac aacaaaagaa gaagaaatgc tttccgagaa
    45421 actgcttgcc acagacgctg tgacaaatac gtctttcatg tccaaacaaa tcgaaacgca
    45481 agaagaatca atggcgaact tagactcggt tgtcttaatc tttgtcgtat tatcaggact
    45541 gctggctttt atcgtcttgt ataatctaac taatatcaat atttccgagc gtgtacgcga
    45601 attatctaca ataaaagtcc ttggattttt tgataaagaa gtcacgatgt acattgttcg
    45661 agaaaatatc atctttactc ttttaggaat catcggagga tttggtgtag gatatatatt
    45721 gactgacttc atcttgcaac aagcgtctat ggagaatgtc atctttccat tagtgatcac
    45781 atggtgggct tatgtcttat cagcaggatt aactatcgta tttacggtta ttgtgatgat
    45841 tgttacccat ttcaaattaa agcatatcga tatgattgat gcgttgaaat cgaatgaata
    45901 aaaacagagg ttgtgaaatc agcggtttga cctgagtatt agagaaacaa acggcaaaat
    45961 agtttttcat attttgccgt tttttgagct aataccgaag gacagctgat tgaacaccat
    46021 gaatcagagg ttgtaaaaaa gccgtcctgc atcgagcaaa aagaatactc aaataaaatt
    46081 agctcctcat gttttacatt tgagaagact gattgccgta gataaaagct tttgaacacc
    46141 atttatagaa gaaacaagaa tattttgcta ttttaaacaa gaggatagac aaatacatgt
    46201 ctgatctctt gttttttatt gggcaacaga cctcttagaa tgtataaaga taaattttac
    46261 aaacgctttc ggttcagtta taataataaa tgataacttg tatatataag ttgtggtagg
    46321 taataaatca gttgaatgga ggtaaatatc catgacattt aaagacaaag tcatttacca
    46381 aatctatccc aaatcttaca aagacagtaa tggtgatgga attggagatt taaaaggaat
    46441 caaagaaaaa ctcccgtatc tgaatgatct aggcatcgac atgatttggc taaaccctat
    46501 ttatccaagc cctcaaaaag acaacggtta tgatatctct gattacacag ccatcgatcc
    46561 tatctttggt acgatggagg aattcgaaca gctaatagcc gaagcaaaga aataccggat
    46621 agaaatcatg ctcgatatgg tgctgaatca cgtttcgaca gaacatgaat ggttcaaaaa
    46681 agcactagca ggggaccagc attatcagga cttcttcatt ttaagagaag agccaacgga
    46741 ttgggtatca aaatttggtg gaagtgcttg gtcgccattt ggagatacgg ggaaatatta
    46801 tttgcactta tatgatcgga cacaagctga cttgaactgg cggaaccaag cagtaagaga
    46861 ggaactattc aaagttgttc gtttttggat ggaaaaaggg gtaaaaggat tccgtttcga
    46921 tgttatcaat gttatcggta aagacgaaca attgaaaaat aataaagaaa atgatggcaa
    46981 acctgaatat acggacaaac caatcaccca tacgtatcta aaagaattga accaagcaac
    47041 gtttgggacg gattccacta tcatcacagt cggtgaaatg agctctacga cgatcgaaaa
    47101 ttgtatcttg tatacagaac ctagtagaga agaattatca atggtcttta atttccatca
    47161 tttgaaagtc gattatgaaa atgggaataa atggtctact cctccatttg attttgcaga
    47221 attaaagcga ctatttcata catggggaga aaagatgagt gagggtaacg gctggaatgc
    47281 tctatttttt aataatcatg atcagccacg tgcgctcaat cgatttgtag ctatcgatca
    47341 gtatcgaaaa caaggagcag agatgctagc aaccatgatc catttgaacc gtgggacgcc
    47401 ctatatttat atgggagaag aaatcggcat gatcgaccca gactattcat cgatttccga
    47461 ctatattgac gtagaaagtt tgaatgctta taagttactc cttgaacaag gattcacaga
    47521 aaaagaagcg tttcgtcgga tcaaagcaaa atctcgcgat aactctcgaa cacccatgca
    47581 atggtcagac acagaacaag caggatttac tacagggaaa ccttggttaa aagttggagg
    47641 caagctagaa gagattaatg tagaaaaaga actatcttct gaagattcta ttttgtccta
    47701 ttacaagaaa ttgattcggc tgagaaaaac atatccaatc gttgcacaag gagattatca
    47761 tgcttatgaa gcaaatcatc cggaagttta tggctttctt cgccaatttg aaggacagca
    47821 gctgcttgta ttaacaaatt tctatgataa agatacaagg atcgccatcc cagaaggatt
    47881 cacagaagcc aatattctta tcgataatta ttccgatact gttcttcatc aaagtctgga
    47941 gctaaaacca taccaggcaa ttgctttgct gaaaaattaa aacaaaaaca caaaacgtcg
    48001 ttcctacgag ctattctcgt aagatcgacg ttttgtgttt tatttaaaat aatcttcttc
    48061 agaaaatgaa gagttttcat actctccttt atcgccataa aatttattgt agcgttgttt
    48121 gaatttacgg aaaataaaag taacgagtaa tttgactaac gtatagatag gaataccaaa
    48181 aataacgccc acaattccca ttagatcacc catgacaagt aaaacgatta agatcgtaat
    48241 cggatggatc tgtaaacgat cacccattac tcgaggaatc accaagtcac cgtggagaag
    48301 ttgtacggca aaccagacaa tgacaaattt gacagccata aaagtagagt cttggaatgc
    48361 aatgatcaaa cctggaaaga aggcgataaa cggcccaata tatggaatga tacataacaa
    48421 cccagcagaa atagctaaaa cactagcaaa ttctaatccg atcagtaaga aacaaaccca
    48481 gtagactgct ccaaggataa atgaagcaat gatttgcccc tttagaaatg aacccaattg
    48541 ttgattagca atctttgtca aatttttaaa atctgtacga aatgctggtg ggacaatcgc
    48601 taaaactgac tgataaaatt tttgaggatc tctcaaaagg aaaaaagcga caattggacc
    48661 agtaaataaa gtaataaaaa tagctgtaac agttgaaaaa atgttgctga tccgttgtgc
    48721 gccactttcc caataatttc caataaatcc agctatatca gacgtcacgt cgtcaataga
    48781 agcgaaaaat tgattaaatg aatcggcaaa aggtgttttc tccaaaaatt gtcggagact
    48841 ctcttgaaaa tcttgaaaca aatctggcaa actactgatc aatgtctttg cctgttcttc
    48901 aagaaaagga ataagccata tgcttccaag ccccaataaa aataaaataa tcacataaac
    48961 gataatgact gacaagttcc tcgaaaggtg cttgctcaaa cgtttgatga ctggttccaa
    49021 aagataatac aagatcagtg caaacaataa tgggggcaag atagtagcga taatgattcc
    49081 gacaggcttg aatataaacg agatctgatg gaaaaaataa atgatcagcg caagtaagat
    49141 aactattcct aaaacatagt aagaggtttt tcctcctaaa aagcggatca aacgattcgt
    49201 tgtatctgtt tcttttttca ctctttttcc tcctttatct ataacttcat tatagacaaa
    49261 agcgggtata aaaggaaaat gatagaggtt gattgcaaaa atatcgtcaa tcattcatat
    49321 aaactttctc atcaattaaa ataagaaaac gcttgacagt tcttttaacc atatgataaa
    49381 ttaaatcctg ttgctaaaac gtaactatta cggtttgcga aaagcgaatc ggaacattca
    49441 aatgaaagaa gatgagaaga ggagtgtcat aaatgaaacg attgataggt atattggcca
    49501 tgctggtttt agcaggtatg ttaactgctt gcggggcaag tgggaaagcc gaggacagta
    49561 aagaaaaatt gagcgtgatg acgacctttt atcccatgta tgattttaca aaagcaatcg
    49621 tcggggatga aggagaagta gagttattga ttccagcagg gactgattcc cacgattatg
    49681 aaccttctgc taaagacatg gcaaagatcc aagatacaga tatctttgtt tataatgatg
    49741 aaaacatgga aacatgggtc cccgcaatcc aaaaaacatt acaagaaggc aatgtccata
    49801 cgataaaagc gacagaaggt atgcttttac ttccgggaag tgaagaagga catgatcatg
    49861 accatgaaca cggggaagaa ggccatacac atgagctaga cccacatgta tggctagctc
    49921 caagtttagc aatcaagcag gtagcaaaca tacgtgatca gctgattgaa gcttacccag
    49981 aaaaacagga agtatggaca aaaaatgcag cagcctatac cgagaaactt caagcactcc
    50041 atcagcttta tcaagaaacc tttaagcaag caaaacaaag atcttttgtc acgcaacatg
    50101 ctgcattcaa ctatctagcg ttagaatatg gattgaatca agtatcgatt gctggtcttt
    50161 cttctagtga agaaccgtct gcggcgagaa ttgctgaact gaaacacttt gttaaagaac
    50221 atggaatcaa ctatatttat tttgaagaaa acgccaaaga tagtattgcc agaacgttag
    50281 caaacgaagc aggtgtatct ttagaagtat tgaatccttt ggaaggattg acgaatgaac
    50341 aaatagaaaa tggagaaaac tatctttcta tcatggaagc aaacctcgaa gcgttgaaaa
    50401 aaacgacaga gacagaaaac ccgctagaag atactttgac ccccaaaaaa gaaaagagcg
    50461 tctacaacgg ttattttgaa gacgaggatg tacaagatcg ctctctttca gattgggcag
    50521 gaacttggca aacagtagat acgtatgtag aagatgggac ttttgatcct gtttttgaat
    50581 ataaagaaaa attgaatcag gataagacag cacaagaata taaagaatat tatacgaagg
    50641 gctatcaaac agatattgct tcaatcaaga tcagtgagaa agagatggca tttacgttta
    50701 agaatggaac aacgaaacgt tcaaattatc gttatgtagg gaaagaaatc ttgacctacg
    50761 aggcaggaaa ccgtggcgta aggtatctat tcgaagcaga agataaggaa agtggcgctt
    50821 ttagatatat tcaattcagt gatcacacga ttgcaccggc tgactccgaa cattttcatt
    50881 tatatgcagg aaatgaaagt caagaagtat tgctgaaaga aatggagaat tggccaacat
    50941 tttatccaga tacaatggat ggacgtgaaa ttgcacagga aatgcttttg cactgatagg
    51001 caaaaaagaa ccgaagggtt tggatcggct ggtcatttag tcggtccaaa ctattcggtt
    51061 ctttttatga aatcaatttc tttaatccac ggatagaggc tgggatggtc tcgccgtttg
    51121 ccaacgtgac ttctctggtt tttttattaa tcgaccgcac attttgtgta ttgataacat
    51181 aggatttatg acaacggata aaattccgat gcattttctc gatttctttg atcttctcat
    51241 agaattgtaa ttgggcatga gtcaaatgga cttccagttt atgtggcgct cctagcgaag
    51301 tagaaaaata ttggatgtct ttgatcggta aaaatttgat ctcatcatta aactctattt
    51361 tgaagatatc tttttggatt tgggacattt gaaaatcttc gaaaattttg ctcaggatat
    51421 gctcgacatt ttcttttaaa tcataaagat cttctttgac gatataatca gtcggttcga
    51481 tgttgctttt taagattttc atcgacattt ccgtatggga agtcacatag attatttttc
    51541 ctaaaggatc taattcacgg atcaatttac cgatcgctac accatccatt tcactgtctt
    51601 ctagttcgat atctaaaaag taaataccaa aagaagaagt tctgatcttg gctgcattga
    51661 gaagttcttc cggatcagag gtagaaatat ggacataact gtctaagttt tcaatcatga
    51721 ttctattttt gatcaccgtt gataaaactt ctaattgttt tggatcgtct tcacaaatat
    51781 aaatgggaat catatcatta ctcctttatt ttatttgtat ataaatcgtt tgagaaaaac
    51841 gctggttttc aattgatgtt tcaaggaaaa gataaggatt ggtctttgtc aattcatcca
    51901 agataaacaa gccaaggcct tcgttttttg gatttgtttt tgttgagaac cctttttgtt
    51961 tcaactggtt taacggtaat gtatttgttg ttgtattgat cacacgaatc atcacggaat
    52021 gattcttctt gagtatggtc aattcgattt ttttcttctt tgcagcacta gctccttcaa
    52081 tcgcattgtc caagataata ccaagcatac gtacaaggtt taccgtatgt ttcgtatcca
    52141 gttcgattgg ctcaggaaaa tgaagctgga cgatcaaccc tttttcctgt gccatcatga
    52201 gttttaatga cagtaaacta cggatctcag gaactttcat attatccaac atacttagat
    52261 tggccggaaa aaggttcatc atctggcgtg taggtaatat cgtttgatca taataagacc
    52321 gtaatccttc taagtcgcct tctgaaatat agctgtgaag agaaaaaagc agattttggt
    52381 aatcatgttt gaattctctc aagtcttgat agtttttttc tagatctttg atataatcct
    52441 gttgcatcct gacttgttgc tctaataact gatcagctaa tttcttatta gagattttcc
    52501 aataataaac caagatgatc aggccaacac acagatatag aatgaaaaaa ctcgtattca
    52561 aagctaagac atcaggtgtt tcaccagaaa aacgggtata aaaaataatg atatagtatg
    52621 taaataatat caatgtcccg atcgtaccga tcatacggcg gtccacagtg tgaaaccaag
    52681 tctctaacga tttttttaaa caaaaagcta ataaagcaga taaaaaagcg gataagacaa
    52741 tgtgaaaata ctgcaatgaa tctcctggtt caataaccga agatttcaaa acaaaaaaat
    52801 cgccgatgct tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa agaaagcgcg
    52861 taaaccaatt gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata
    52921 gcaattagag cagtaaaaat ccctaaaatc gaatagacct atccaatcaa aatacaagaa
    52981 atcagaatga ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata
    53041 caaattgtgt aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc
    53101 cattgatttt taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat
    53161 tttgaattca gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt
    53221 taattgttta atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg
    53281 taataggaag caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt
    53341 aagaagctga gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct
    53401 ttaataatgg gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat
    53461 agcgaagcag taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag
    53521 tcaattgcag atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca
    53581 gacaaacgaa ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag
    53641 aaagaaaggt gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga
    53701 ccgctagttt tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca
    53761 ccatatcttc ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt
    53821 tatctttcat ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt
    53881 cttttgtttc ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct
    53941 tcgttttcca agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat
    54001 gacaagtttg tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg
    54061 atggagggat tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta
    54121 aatctgattt atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg
    54181 tgatgatacg tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact
    54241 ggcagttagt tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag
    54301 agcataaaga ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa
    54361 acagcttttc atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg
    54421 tttatttgaa aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg
    54481 agttactttt ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt
    54541 tatttctata aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat
    54601 aattgtgatc attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct
    54661 tgcccaagat ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc
    54721 gctattttat tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa
    54781 acgctttttg gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat
    54841 ctacgaagat agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga
    54901 agtaaagcag tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc
    54961 acaaaagaaa ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag
    55021 aatcaaaaaa agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta
    55081 ctataaagaa ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa
    55141 aatagtgatc gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat
    55201 cgtacaagaa atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat
    55261 ccgctacact acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa
    55321 ctaagcaaat tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt
    55381 gattcattga gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt
    55441 ctgagtgagg gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa
    55501 cagatgatga cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag
    55561 atagtggaaa aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt
    55621 cgttgctacc ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat
    55681 tttatcaaaa gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc
    55741 gggatgctca tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag
    55801 aacaaacaag cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt
    55861 tagaagaatc cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca
    55921 ttctccgtca aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa
    55981 agcaccatat tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag
    56041 tcctattgta cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata
    56101 tttttgtctt ccttatctta gcataaacga ttgaaaaaaa ggaaaaattt tcagaaaact
    56161 aaaatcatat cgcttcccat tttcattttt agaaaggtct gttagtataa agaaaaaata
    56221 gacaacgaaa ctttaaataa ccaacttgaa ataaacaaat tcaaagaaga aagatgggaa
    56281 ggaaatcaat cagttgaatg aaaaagaaat tctccgatta caaaaagaat tgaaacgctg
    56341 ggagaacaag tataaaaagc ttcaacaagt ctttcggcta caagaagaaa tcattgctac
    56401 ctatgaagcg gcttatggcg aagcacccgg acttattgag gaagaagtac aagtaaaaga
    56461 aagtgaagga aacagctctt tctttaaaat gatggagaaa cgttcctata atcgcttcaa
    56521 tcaagaacaa aaagcagcta tcacatatga tatgaagaaa catcttcgaa tcatagcagg
    56581 agctgggagc ggcaaaacgc aaacaatctg tgcaaaagct gtttatctga tgacgcaaaa
    56641 acaagtagac gaagaacgca tactaatgat cacttttaca aggaacgctg caaatgaact
    56701 aaaaaaacga gtagataatt tcagccagag aaaaaccgct gtccatatcg gaacattcca
    56761 tagtatcttt tttaggatat acaatgaaat ttgtcgaaaa tttcctgaag tagcaatgca
    56821 aggaatacaa ggagactttt caaaggatac tgcacaaaaa gtgaatgctg ttttgcaaca
    56881 gctgatacgt aaatataatc tttatatatt tgatcaatat ggagaaaaaa cgattgcttc
    56941 tcggttagac tattggcaga atatgaacct ttcagtagaa gaaatggttc agttagttaa
    57001 agaaaaatat gattccattg ataagaatgc acgacagccg atatcagaac gactgtatgg
    57061 attgctcacg gaactccaag aacaaaaaag aagccagcaa ttgctagagt tcaatgatgt
    57121 actccaaaat ctgaaaagcg ctttggagaa tgaggagatc cagcattata tcggccaaaa
    57181 atatgattat ctattcattg atgaatttca agatactaat ccacttcaat gggaaatcgt
    57241 gaaacggatg acaaaggaaa acaggatcaa gttgattgtt gttggagatg atgatcagag
    57301 tatctatgga ttcagaggat cagagccagc atacatcaag aactttgaaa aagaatatcc
    57361 taccaaaact cttttcttat taactaatta tcgttctcgg gcagctattg tccaaggagc
    57421 aaatagattg atcagctata ataaaaatga tcggatacca aaggcgatga ttccagcaca
    57481 aaaagaagca ggtgttatgg aagcctattt gttttctgat actaaagcag aaagtcagtg
    57541 gctaatcaaa caaattcaga catttattca aaaaaatggg aaatataagg aatcaatcat
    57601 attataccga tcagcttcac aaacgcagca gcttgtccaa tctctgctga aaacgaatat
    57661 tccttttgta ctggaagcag atagtccata tgaaggtatc tttggtatca agtctttcca
    57721 acatttctat caaaaaattg ttcattggca aacagcactg aatctgcaaa agaaaaatca
    57781 ggcctaccag caattgctcc ggcagctgat ggcagactgc tacctgaaaa aaagtgaatg
    57841 cgatcaatac ttttctccta aatatcaaaa tatatccgtt gcagattata ttttgtcgat
    57901 aagccccaat ttaaaatcga aatctgcaga aatccgagag tttgaggaag cgttgcgtaa
    57961 aacagaccag catcaagaaa tctattcgct tgttcaaagc tatcttcatc tgccacgtat
    58021 agctaaggaa atcgaccaga gtgatcaaga atggatacga gaagaagtaa agcagcatgc
    58081 tactttcaga agactccaag ccttatcgca agaaacccaa gcgacagcta aagaattaaa
    58141 aacccgcttg attgcttaca gacaaggaaa acttgatgca ctatgtatcc agagcattca
    58201 caaaagtaaa ggtcttagtt accgaaatgt ctttttgatt ggttgtaacg aaggaagttt
    58261 gccttataac ggagcgacag aaaagaaagt aatcgacttt aatgaaataa aagcagaacc
    58321 agtaacgacg atagaagaag aacgaagact attttatgtt gcgatgacta gagcaagaaa
    58381 tcgtctgtat ctatgtgtac ctcaaatgaa aaattcaaga aaattgaaag tatcgagatt
    58441 tatcaaagaa accgggagtc gtgtgaaaaa aggaaagtga tagaagagag ggtgtgacag
    58501 aagtggtcag tttcaagaaa gaagaaggaa tgtacgaaaa ttgcttctca aatttttgtg
    58561 aaatttcagc ttattttcga agaagctatt tctggaacac agtttattcg gaaaagggag
    58621 gtgtgacaag aattttgtca cacctccctt atttattttg aaaatgagaa tactattatc
    58681 aaaatagaga atatatagaa aaattctcat tattcaaacg taaataatca gttattttga
    58741 ttttttttat attttattag ctaataattg gtattttaaa agaaattact gtagaattgg
    58801 attataatta aaaaggagtg agaaaatgat aaaagtatat acttcgccta gctgtacttc
    58861 ctgtagaaaa gcaagagcat ggttgcaagc gaatcaactt gaatttgaag agaagaatat
    58921 ttttgccgaa ccgttaacag aaagtgagat caaaaagatc ttggcagcga ctgaaggagg
    58981 agttcaagat attatttcca ctcggtcgaa aatttacgaa aagctgggca tagattttaa
    59041 cgaattaact ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat tattgagacg
    59101 acctattttg attgatgaaa aaagaatact aatcggattt aatgaagatg agatccggtc
    59161 atttgtccct cgtgaaattc gatgtgttgc cagaaatctg atctatttac aaaacaataa
    59221 catcttaaat tcatagggag ggagccttcg tggaagtgaa agtctatgga tcagcgcact
    59281 gtgtaacaag tctgaaggcg aaacaatggt tagaacacca tcaattgcct tatcaatttg
    59341 ttgacttaga agaacgcgat ctaagtacag aagaagcaga agaattatgc agttttgaag
    59401 aagtgcaagc agaacagcta tttgcgactt ggtctgaggc ttttcacaac attgcgattg
    59461 acctttcctg tgtaggaaaa aaacaactga tttttttatg cacacaacat accaatctcc
    59521 tcagacgtcc tttgattatt atcgatgaag ttctatttat tggatacaat gagcagttga
    59581 tggaacagct tatcttaaat aaatagactt taactgtttt aatcaagtca ctgagcattt
    59641 catacagttg taacagtcga acacttagca tcaagcgtgt tatgtgttct ttttttgact
    59701 ttatttttct actatagaga catgctcaat ttatgtggtc aaaattcaaa tctaggtggg
    59761 gatatagagt taaaaatata ttgacatgtg taaatttaca tgatatattc atttctgttg
    59821 ttttaagtag caaagcaata aaatagtatt ttaaaaaaca tataaaatag ttgttgacat
    59881 gttagaaatg acatgataag ataactgagt tgttaaaaaa gaaccacttg
//
LOCUS       DACEPD010000006        58090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.006, whole genome shotgun sequence.
ACCESSION   DACEPD010000006 DACEPD010000000
VERSION     DACEPD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.006"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(189..1475)
                     /locus_tag="IW797_000331"
     CDS             complement(189..1475)
                     /locus_tag="IW797_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1223132.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1468..2736)
                     /locus_tag="IW797_000332"
     CDS             complement(1468..2736)
                     /locus_tag="IW797_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ1223133.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            2988..3449
                     /gene="mscL"
                     /locus_tag="IW797_000333"
     CDS             2988..3449
                     /gene="mscL"
                     /locus_tag="IW797_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ1223134.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3508..3954)
                     /locus_tag="IW797_000334"
     CDS             complement(3508..3954)
                     /locus_tag="IW797_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1223135.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4047..4292)
                     /locus_tag="IW797_000335"
     CDS             complement(4047..4292)
                     /locus_tag="IW797_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223136.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4407..4985)
                     /locus_tag="IW797_000336"
     CDS             complement(4407..4985)
                     /locus_tag="IW797_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1223137.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5062..5592)
                     /locus_tag="IW797_000337"
     CDS             complement(5062..5592)
                     /locus_tag="IW797_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ1223138.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5596..6456)
                     /gene="rlmB"
                     /locus_tag="IW797_000338"
     CDS             complement(5596..6456)
                     /gene="rlmB"
                     /locus_tag="IW797_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ1223139.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6449..6844)
                     /locus_tag="IW797_000339"
     CDS             complement(6449..6844)
                     /locus_tag="IW797_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1223140.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6841..8253)
                     /locus_tag="IW797_000340"
     CDS             complement(6841..8253)
                     /locus_tag="IW797_000340"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ1223141.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8250..8786)
                     /gene="cysE"
                     /locus_tag="IW797_000341"
     CDS             complement(8250..8786)
                     /gene="cysE"
                     /locus_tag="IW797_000341"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ1223142.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(9108..10568)
                     /locus_tag="IW797_000342"
     CDS             complement(9108..10568)
                     /locus_tag="IW797_000342"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ1223143.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10738..11895)
                     /locus_tag="IW797_000343"
     CDS             complement(10738..11895)
                     /locus_tag="IW797_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ1223144.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11929..13299)
                     /gene="radA"
                     /locus_tag="IW797_000344"
     CDS             complement(11929..13299)
                     /gene="radA"
                     /locus_tag="IW797_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ1223145.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13362..13889)
                     /locus_tag="IW797_000345"
     CDS             complement(13362..13889)
                     /locus_tag="IW797_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ1223146.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            14119..14925
                     /gene="proB"
                     /locus_tag="IW797_000346"
     CDS             14119..14925
                     /gene="proB"
                     /locus_tag="IW797_000346"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ1223147.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14922..16169
                     /locus_tag="IW797_000347"
     CDS             14922..16169
                     /locus_tag="IW797_000347"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ1223148.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16245..16589
                     /locus_tag="IW797_000348"
     CDS             16245..16589
                     /locus_tag="IW797_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223149.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16706..17032)
                     /locus_tag="IW797_000349"
     CDS             complement(16706..17032)
                     /locus_tag="IW797_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223150.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(17405..18606)
                     /locus_tag="IW797_000350"
                     /pseudo
     CDS             complement(17405..18606)
                     /locus_tag="IW797_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            18723..19238
                     /locus_tag="IW797_000351"
     CDS             18723..19238
                     /locus_tag="IW797_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1223151.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(19437..19883)
                     /locus_tag="IW797_000352"
     CDS             complement(19437..19883)
                     /locus_tag="IW797_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1223152.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(19930..21408)
                     /gene="msr"
                     /locus_tag="IW797_000353"
     CDS             complement(19930..21408)
                     /gene="msr"
                     /locus_tag="IW797_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1223153.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(21762..22391)
                     /locus_tag="IW797_000354"
     CDS             complement(21762..22391)
                     /locus_tag="IW797_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1223154.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(22375..22839)
                     /locus_tag="IW797_000355"
     CDS             complement(22375..22839)
                     /locus_tag="IW797_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1223155.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(23326..24594)
                     /locus_tag="IW797_000356"
     CDS             complement(23326..24594)
                     /locus_tag="IW797_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1223156.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(24825..25562)
                     /locus_tag="IW797_000357"
     CDS             complement(24825..25562)
                     /locus_tag="IW797_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1223157.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(25629..26198)
                     /locus_tag="IW797_000358"
     CDS             complement(25629..26198)
                     /locus_tag="IW797_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1223158.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            26326..27198
                     /locus_tag="IW797_000359"
     CDS             26326..27198
                     /locus_tag="IW797_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1223159.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            27540..28412
                     /locus_tag="IW797_000360"
     CDS             27540..28412
                     /locus_tag="IW797_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1223160.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            28409..30367
                     /locus_tag="IW797_000361"
     CDS             28409..30367
                     /locus_tag="IW797_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1223161.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(30618..31469)
                     /locus_tag="IW797_000362"
     CDS             complement(30618..31469)
                     /locus_tag="IW797_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1223162.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            31592..32059
                     /locus_tag="IW797_000363"
     CDS             31592..32059
                     /locus_tag="IW797_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1223163.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(32322..32804)
                     /locus_tag="IW797_000364"
     CDS             complement(32322..32804)
                     /locus_tag="IW797_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1223164.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(32904..33767)
                     /locus_tag="IW797_000365"
     CDS             complement(32904..33767)
                     /locus_tag="IW797_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1223165.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            34365..35213
                     /locus_tag="IW797_000366"
     CDS             34365..35213
                     /locus_tag="IW797_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223166.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(35384..35725)
                     /locus_tag="IW797_000367"
     CDS             complement(35384..35725)
                     /locus_tag="IW797_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ1223167.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            35811..36518
                     /locus_tag="IW797_000368"
     CDS             35811..36518
                     /locus_tag="IW797_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ1223168.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            36505..36828
                     /locus_tag="IW797_000369"
     CDS             36505..36828
                     /locus_tag="IW797_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ1223169.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(36917..37954)
                     /locus_tag="IW797_000370"
     CDS             complement(36917..37954)
                     /locus_tag="IW797_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1223170.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(38401..39384)
                     /locus_tag="IW797_000371"
     CDS             complement(38401..39384)
                     /locus_tag="IW797_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ1223171.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(39800..40570)
                     /locus_tag="IW797_000372"
     CDS             complement(39800..40570)
                     /locus_tag="IW797_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1223172.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(40563..41498)
                     /locus_tag="IW797_000373"
     CDS             complement(40563..41498)
                     /locus_tag="IW797_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1223173.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(42092..43741)
                     /locus_tag="IW797_000374"
     CDS             complement(42092..43741)
                     /locus_tag="IW797_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1223174.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVCSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(43936..44832)
                     /locus_tag="IW797_000375"
     CDS             complement(43936..44832)
                     /locus_tag="IW797_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1223175.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(44825..45505)
                     /locus_tag="IW797_000376"
     CDS             complement(44825..45505)
                     /locus_tag="IW797_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1223176.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(45506..46288)
                     /locus_tag="IW797_000377"
     CDS             complement(45506..46288)
                     /locus_tag="IW797_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1223177.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(46281..47198)
                     /locus_tag="IW797_000378"
     CDS             complement(46281..47198)
                     /locus_tag="IW797_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1223178.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(47201..47485)
                     /locus_tag="IW797_000379"
     CDS             complement(47201..47485)
                     /locus_tag="IW797_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1223179.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            48273..51500
                     /gene="fss3"
                     /locus_tag="IW797_000380"
     CDS             48273..51500
                     /gene="fss3"
                     /locus_tag="IW797_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1223180.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            51613..51927
                     /locus_tag="IW797_000381"
     CDS             51613..51927
                     /locus_tag="IW797_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1223181.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            51940..52314
                     /locus_tag="IW797_000382"
     CDS             51940..52314
                     /locus_tag="IW797_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1223182.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            52324..52659
                     /locus_tag="IW797_000383"
     CDS             52324..52659
                     /locus_tag="IW797_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ1223183.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            52742..54082
                     /locus_tag="IW797_000384"
     CDS             52742..54082
                     /locus_tag="IW797_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1223184.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            54160..54834
                     /locus_tag="IW797_000385"
     CDS             54160..54834
                     /locus_tag="IW797_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1223185.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            55067..55501
                     /gene="dcm"
                     /locus_tag="IW797_000386"
     CDS             55067..55501
                     /gene="dcm"
                     /locus_tag="IW797_000386"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1223186.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            55502..56209
                     /locus_tag="IW797_000387"
     CDS             55502..56209
                     /locus_tag="IW797_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ1223187.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            56199..56489
                     /locus_tag="IW797_000388"
     CDS             56199..56489
                     /locus_tag="IW797_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223188.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            56748..57932
                     /locus_tag="IW797_000389"
     CDS             56748..57932
                     /locus_tag="IW797_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1223189.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            57947..58066
                     /locus_tag="IW797_000390"
     CDS             57947..58066
                     /locus_tag="IW797_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1223190.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT        17175 a        11874 c        10852 g        18189 t
ORIGIN      
        1 tcacaacctc tttgattact tcgtttgatt ttattttaat ataagttgat taaaagagag
       61 catgatttgt tttattttct cactaatttt tccaacacaa ctaagaatac cataaaagct
      121 gattttggag gaggttccta ctacaagtct aaaacgaaat ttaagacttt tcctcataaa
      181 atactggttt attttggtct catgtaaaac tcgctgaagg cctcttcact tataaatgct
      241 tccccagctg ccagtacgtc tttcatttgg atcgaatcga tgatttcagg aagatcaaat
      301 aaagttcgat cgccaaataa actttgcgta aattgattgg caatatattc gatcgaattt
      361 aatgattgga aatattgtcc aagcattttc ttcttcagca aatcgagatt tgtttctgac
      421 acttccggat catcagcgaa tccaagaatg atttctttga ctttttctgc tgctttctct
      481 ggttcgtccg tatctccgct gaagtccgca aagtgaaatt cacgatccaa gctgaattca
      541 aaaccaaagc tgtcatcgat aattccttga ttgtacatcg ctagatagtt tcgagaagta
      601 ttgcctaaaa tcatttggaa caacagattg attgctgttt tatagcgtaa cagttctgtt
      661 ccttcttgcg gcaatgtgtc taatccttta ataccaagta caaatttatc tcgtgtaatg
      721 gctgcttcca atgcactttg tttgatgatt tcttttgtgt tttcagggaa ataacgtaca
      781 atttcttgct ttggagggaa attttttgct tcttggtttt cccgaataag tttcatcaat
      841 ttctcaggct ccatcttacc aactacaaat aaaaccatat tgcttggctg ataaaacgtc
      901 cgatagcacg tatacaagtc ttgagctgtg attttgtcga tactttcaac cgtgcccgca
      961 atatcgatat gcaatgggtg tgtaggatac aagttattta gtatgccaaa aaacatccgc
     1021 cagttagggt cgtcttcata catttggatt tcctgaccga tgattccttt ttccttgttt
     1081 acagtttctt ctgtgaagta aggtgcttgt acaaaatcga tcagagtcgt cagattcttt
     1141 tctacctgat ccgtcgtaga aaacagatag ctggttttcg taaaacttgt gaaagcattg
     1201 gcggaagctc cttgtttccc aaataattga aagacatcac catcttcttt ttcaaacaac
     1261 ttatgttcta aaaaatgggc aattccatct ggaactttca ccttttcttt ttcaccatag
     1321 ggaataaatt cgttatcgat cgaaccataa ttcgtactga acaaaccata tgtcttgtga
     1381 tagtcatttt taggcaacaa atacacggtc aaaccgtttg gcagtacttc atgataaagt
     1441 gtttcattga tttgctcata ttctgtttta ttcatttgca tttcctcctg ctaaaaagaa
     1501 gattgcctgc aattcgattt gctcagctac ttgttgtatc tctttgatcg ttacgctctc
     1561 catacgtttc aaccattctt catcagataa ttttgtttcc ggcagccagc tgtccaaaaa
     1621 agaagcttcg attgccgctt gcggagagtc tagagaaagc agatattggt ttctcagcat
     1681 tgcttttgtc tgagacagtt ccaactctgt tatttcacca tttcgcaaac tttctaattg
     1741 ctcgtgaatc agatgaagta cttgatttcg gttcttttca tcgataccgg tttggacact
     1801 catgaatcct cggaacgtat ccacgctact agaggcataa taagctagac tttctttctc
     1861 tctgacattc atgaaaagtt tagagtgagg aaaaccgcca aataaaccgt taaagaccat
     1921 tagcgcaaaa cgttctggtt catcgtaata cacatttgtc tgataagcta ggttcagctt
     1981 tgcttgaacg attggctcac gtacttgtcg ttccttcacg atattcactt gaggttgggt
     2041 ataaaacaga tctggataaa ctgtttcccg cgactcaaat ggtaattgcc caactgcttc
     2101 agctgctttg tccggatcaa catcgccgac aacaaaaatg tcgatttgat catttgccat
     2161 catttcttga tagtaggacg ctagcgaact agcagtgatc ttgtccaaat cttcgactac
     2221 gcccaaccct gggatctttt gttccgggct gttttcaaaa taaagttctt gcaaggcata
     2281 ggaagcaaaa gtttgtttat cttccttcaa actttccaaa tatagacgca gattgttctt
     2341 ttccaagtca aacgtcaact gatcaaactg atgattttta atatttggat aaaataaaac
     2401 ctcttttaaa aaatctaccg cttgagaaaa aaggtttgga tcattgacat attcaccatt
     2461 cacaaaactg atccctgcgt ttagccaatg taaatttcct ttttttctaa ctgataatcc
     2521 aaagcttgcg ccataaagct ctgcaagttg cgcacttaac tttgtctgat ccggataatt
     2581 caaactattt gtttccaaca tgctgctgag aagtgaacgt ttcgtgatga cttcttgttg
     2641 cagacgcgca gtaaaacgga taaaaatccg gatcgttttg tattgttttg ttggtaagac
     2701 atgaagattc acgccttctg ccaattgata caccatattt atatgctcct tttcacagtt
     2761 gattgcccgc ttactctttt aatgggtcaa cctgcctttt ttacttattc ctctgcttga
     2821 aatttataag ttcaggcaat tcgccgtttt ttctaaatga tgttacactt tatagtatag
     2881 catagatgtt ttttatcaaa agaaacagac ggctaaaagc gtatattgtt tttttatttt
     2941 ttatttttcg atatactaaa gacaaataaa agaagggagg gtcattcatg atcaaagaat
     3001 tcaaagagtt catcatgcgt ggagatgtct tggatcttgc tgtcggcgtt gttattggta
     3061 gtgcgtttac aagcatcgta aaccaagtgg tgcaaggctt gatcacacca ctaataggtt
     3121 ggtttgtcgc aatgatcaca ggtacttctg atttagaagg agcactttca attcttgact
     3181 ggtcgccgac aaaaggcgtc acatttgcgt ttggtaacgt tattagtgct atcattacat
     3241 ttctgatcac tggttttgtc ttattcttag ttgtcaaagc ggcaaaccgt gcgaagaatt
     3301 tacgctccaa agaagagcaa gtggaagtgg aagaaactcc tactgcagaa gattatctta
     3361 gtgacatacg cgatcttctt gccaaacaag tggcagaaga caatacacat ccagtagagc
     3421 aaacaaatct ttccgatact tctaaataat aaagaatcta aaaaaaagaa agccgatttt
     3481 cagtgtcggc tttctttttt tagattctta acttgctact tgttcctttt tcatgtcgat
     3541 atgtggaata tcatcttcca aatacacatc tgagattgcc ttgaatccaa acgactcata
     3601 aaaagcttgt aggtattctt gtgcgccgat cacgattggt ttttctggaa aatttttctg
     3661 gatcatgtta atcgtttctt gaacaatctt tcttcctaaa ccatctttac ggttgtcctg
     3721 atgaacaagg acacgtccaa aatggatact ctccgcttct tcatatactc gcgtgtaggc
     3781 aaggatcttc ccatcagtat cttgaaaaaa cgtatgccaa gcttgtccat caatgtcatc
     3841 aacttcttga taaggacact gttgttccac aacaaaaact gcgattcgtt ctttgacgat
     3901 cgcgtaaaac tctgctgttg acaattcttc aaatttcttt acctgatatt ccactaagca
     3961 aacgccccct gcttttttat tcttagcata ccgattcgca cacaaaaaag caaacgaacc
     4021 ttacgaatct caggctcgtt tgctttttaa actgcttcag ttaaaaattc gatctcaact
     4081 gtccgagtaa gtacatctga atagctgtaa gatactcgtt caaaagaatt ttcttcagga
     4141 tccaaatcaa caacgaatac tgaaggatag gtttctgtta aaacgccgcg gcgttctgtt
     4201 tgacgctttc ttcctgtctg agcaaccaac atgattgggc tgccgatacg accttctaaa
     4261 tctttcttga ttgaagctaa agttgttggc atacagttca cctctactat caactatacc
     4321 acatattttt aaaattttca agtttaccat aaaaaaaaaa gaaatgcaat aaaaataaat
     4381 aagaagctca ttaagatttt tctaaattat tcctcattat aaaatttcaa gtgttttttt
     4441 attttgagtt ttactctccc gtaagcgctc attacttgct tgtgatccat tgctaaacat
     4501 tcagccatgt ctacaaaatc aacgccttgc atataataat gaaaaatttt agcttctatt
     4561 tttgaaaact ggatttcgca attttcgatt gcttctttta ataaaagatg ctgaatcgct
     4621 aaaggttctt gcaatgtgat ctctgctaaa aagtctggtc cttctgtctc tattttttgg
     4681 tcgattgaga cttcatcttt ttgatcacga cgcttcagcg cgttctgctt cctcaacaga
     4741 gaatagattt gattgcttat aatgctttta taataacttc caaagctaac acctttgctt
     4801 ttttcgtaag ttctcaatgc ctgatataaa gcgatccttg cttcttgttc ccaatcctca
     4861 gaaacataat ttcttaaata ataggtgttt ttcaaacgta gtaaaagtgg aaaatatttt
     4921 tgatacaggg cagcaaatgc ttcttcgtca ccattacgtg cagcgcgcaa taactcctca
     4981 gacataaaat aattcctcca taaattcgtt tatggaggaa tcttatcatc aatgattcat
     5041 cgaactgatt agaacgatat gttatttatt ttttctcatt aaatcgtcca atttttggga
     5101 caattttttt aactgctctt cattccatgg agaattacgt cggaaatcaa taaaatgcat
     5161 atctttattt tcttgggcta ttgttttttc cgtttttttt atcgtcttat ataattcata
     5221 agcagaagtt cgtaatgctc cttgggaaaa gatcatccac tgttctgcta aatcgcttgt
     5281 cgccactgtc acttgtgtca aacggtcatt cagctgacca gcgattctct cgatataact
     5341 gtcagccgtt tcgtcttctt tcgtaaaaac aacatccaac tggtatttct tataattttg
     5401 ttggatgccc ggaactagtt gggcatcgaa gacaacgatg acttccattc cttcatattt
     5461 cgcataattt gataaacgat gtaaaagagc ttctcttgca tccgccattt tgtcctgctt
     5521 tttaagctgg acaagttccg gccacgaacc aatcatgttg tagccgtcta caatcaacag
     5581 ctgttttttc atggtctact ccccctgacg ttgggaaaaa gcctgataca taagcaaacc
     5641 agcagcaaca ctggcattta agctctggac gtgcccggtc atcgggatcg tcagcaattc
     5701 atccatttct tttttcaatc ctggactcat tcctttgcct tcattaccaa tgactaaagc
     5761 aattgatcct ttggcattcc atttttggaa gtttgtgccg gtcatatctg tgccaaatac
     5821 ccaaaatcct gtttctttga gctgttgcac agcttgtacc aaattcgtca cgcgtgcaac
     5881 agggacatgt tcgactgccc cagtggaagt ctttgttacg ataggtgtga ttccaactgc
     5941 tcggtgtttt ggtatgatca caccatcgac gccacttgcg tctgctgtcc gtaaaatcga
     6001 cccaaaattg tgtggatctt cgatgctatc caaaatcaaa aagaaggggt cttctttttt
     6061 ctcttttgtt cgttcaatca gttggtcaag ggataaatat tcataaggtg taatcgcaag
     6121 aacgactcct tgatgcacgc cgtgatcgct caaagtttct aatttttgtt taggaaccca
     6181 tttgactggc acagcttgtt cacgtgcgat ttctttcagt tcttctactt tttctcctcg
     6241 tgaatcttct tgaagaaaga gtttattccc tcgcccttgt tgtagcgctt ctactgcggc
     6301 atggtgccca aagacgaaat tatcttctaa tgcttcttca ttttgatcag aaactgactg
     6361 atttcgtcca gtttgctttt tatttgaata tctattttgt tttttgtctt caaatccacg
     6421 tttcgattga tgtttgtttg ttctttcttt acccattttt ttcaccaact ttctgaatac
     6481 accagcggat caattcttcc atccgctcgg tttgttttgt caaatgcaga tagcccatga
     6541 gcgcttcgaa ccctgttgcg acccgatacg tcgtaacatc agcatttttt gcgctcgtat
     6601 ggcttttagc atttcttcct ctgcgataat aaagttcttc ttcttctgtc aaaagatgtt
     6661 cttccagcat ttgctgaatc aaaaaggctt gtgcattcgc agaaacataa ttcgttgccg
     6721 cacgatgcag cttattgggt tttgtttctc cttgatcaat caggtggtca cgaacataga
     6781 tttcgtaaat cgcatctcca acataagcca aggctaaacc gttcaattgt gtgtaatctc
     6841 tcatgcttct cttctccatc gggttccttg cggtgtatct tctaataaga tccctttttc
     6901 tttcaacaaa tcacgaattt cgtcgcttcg gacaaaatca cgattgcttc ttgcttgatt
     6961 tcgttcttcg atcaattgtt caatctcttc atctaataac tcttctgctg taaataaaat
     7021 accgaagatc gaaagccatt gcgagaataa agcttctcct tgtgccaaga cagcggcaga
     7081 aactttcggc tggtctagat aagtgttcag ccacttagca agctcataga ccactgtgat
     7141 cccatttgct gcattaaaat catcgtccat ttcttcgatg aatcgctgtt ctaaattttc
     7201 taacaaagcg attttatccg catcatctgc caaattctct gaggcagttt cttggcggaa
     7261 tttcaaattg tcaaacgtat tcttcagtcg ttgtaaattg gtggctgctt ctttcatcgt
     7321 cgtttcactg tagcggatcg gacgacggta ttgtgtggtc gccatgaaga aacgcaagac
     7381 ttgtggatca acgtttttga tcatttcatg tacagtgatg aaattcccta gagatttact
     7441 cattttttca tcgtcttcgc caatcgttac atagccattg tgcatccagt aattagcgaa
     7501 ttttttccct gttttcgctt cactttgtgc gatttcattt tcatgatgag gaaattctaa
     7561 atcttgcccg ccaccatgga tatcgatcgt ttctcctaaa tgttttgttg ccataacgga
     7621 acattcaata tgccaaccgg gacgtccttt gccccatggt gaatcccaag aaatctcgcc
     7681 aggttttgct tgtttccata aagcgaaatc taatgggtct tctttttgct gctgttcttc
     7741 ccctgtacgc tgacttgctc cgacttccag ttcatcgatg gattggtcac ttaatttccc
     7801 ataattttca aatttacggg tacggtaata cacatcacct gaagattcat aagcatagcc
     7861 tttttcaacc aacacttgaa taaatgctaa aatatctggc atatgatcca tgactcttgg
     7921 atgcagtgta gcaggctcga cattcaacgc tttcgtatct tcttcaaatg ctcggatgaa
     7981 acgatcagca acttctggtg ctgtaatacc taattcgttt gctgtacgga tgattttatc
     8041 gtctacatcc gtgaaattcg atacgaaatc gactttatag cctcgatatt caaaatagcg
     8101 tcgaattgta tcgaaagcaa tggtgctacg agcatttccg atatggatat aattatacac
     8161 agtcggtccg cagacataca taccgacatg tcccttttgt atagggatga attcttcttt
     8221 ttttctggtc aatgtattgt agatttttat cattctgatc tcctcacttt ttctcctttg
     8281 attcgaacga cttttgctgg tactcctacc gctgtagcgt cgtctggaac atctttcaac
     8341 acaacggcac ctgcaccgat tttggcgttt tttccgatcg ttaccggtcc taatatctgc
     8401 gcattcgcag agagcatggc tccttgttta acagttgggt gtcttttacc cgtttctttt
     8461 cctgtgccgc ctaatgttac gccatggaat aaaatcacat cgtcctcgat ttctgcggtt
     8521 tcgccaatga cgattcccat tccatggtcg atgaacacgc cagttccaat cgttgctcct
     8581 ggatggatct caacaccagt taggaaacgc cagaattgag cattgatctt cgccaataaa
     8641 aagagccggt gctgatataa aaaatgagag aaacgatgcc aaaacaaggc atgaaaacca
     8701 gggtatgtca aaacgatctc agctgtcgaa cgtgcagccg gatcgtttct ctttgctgca
     8761 tcaatcgctc gggtaaacca actcatgact ttccactcct ttcgtaagaa ttttatgtag
     8821 gaaatcatct tcttataaag aaaagaagcg ccttttaaat aagtttcctt atcaaaagac
     8881 gctttccgcg tggttccact tttcttcata atcaatgatt atctctgaca gctaacgcct
     8941 gttatacgtt cttggctact ttgtttccta taaaacagtt tttcctgttt tctgtgtttc
     9001 gccaagacca ctcccagagg catttcggca accattcatt gataccttgc accacccggt
     9061 atctctcttg taatgatcag ctgcttactt ttttctgttc atcgtgttta gttcagtact
     9121 tgatttaaat gtgctaatga tttttcacga ccaagcaatt cgatcgtttc aggcaattct
     9181 ggaccatgca tttgtcctga tacagctaca cggattggca tgaaaagatt ttttcctttt
     9241 acgccagttt ctttttgaac agctttgatg gcagctttga cagttggagc atctactact
     9301 tccatttgtt ctagctgtgc tttgaatgcg tggagaacag taggaactgt ttcaccagca
     9361 agtacttctt ttgctgcttc attcaataca ggatgttcat tgaaaaatag cgtagacaga
     9421 tcaacgattt ctgcagcata gctcatttga ggttgataca ggctaacgat cttcttcacc
     9481 cattcgattg tcttttcatc tgggttttct gctacacgtc catcagcgat tagataaggc
     9541 agacacatgt cagtaaatgc atctagatcc attgctttga tgtaatggtt gtttacccat
     9601 tgcagttttt tcgcatcgaa agctgcgggt gatttactta ggcgttttgg atcgaacatt
     9661 ttgatcaatt cttggtgttc gaagatttcg tcttcgccca caggtgacca gcccaataat
     9721 gcaataaagt tgaacattgc atctggtaga tagcctaatt cgcgatattg ttcgataaat
     9781 tgcaagattg attcgtcacg tttgcttagt tttttacctg tttcactgtt gatgatcaat
     9841 gtcatatgtc cgaaggttgg tggtgtccag ccaaatgctt catagatcat cagttgtttt
     9901 ggtgtattgg caatatggtc atctccacgt aaaacgtgag tgattttcat caaatgatca
     9961 tcgaccgcaa cagcaaaatt ataagttggc ataccatcgc gtttttgtat gacaaagtcg
    10021 ccaccgatat tatcggattc gaacgaaata gcgcctttca ccatatctgt aaatgcgtat
    10081 tctgtattgc gaggcacacg gaaacgtaca acagattcta gaccttgtgc ttctttttct
    10141 gcttgttctt caggtgtcaa gttcgcacat gttcctgcgt aatgaggcat ttctcctctt
    10201 gcgcgttgtg cttcccgttc tgcttctaat tcttcttctg tgcaatagca tttgtatgct
    10261 cgattgctag caagcagctg atcaatcagc ggttgataga tttctttacg ttcagattga
    10321 cggtaagggc cgtattctcc aggattttca ggagattcat cccaattcat tcccaaccaa
    10381 gcaaggtttt ccaactggct tttttcgcca tcttcgatat tacgtttttg gtcagtatcc
    10441 tcaatacgaa taataaagtc tccatcattg tggcgggcaa acaaataatt gaataatgca
    10501 gttcttgcat ttccaatatg caggtgccca gttggacttg gtgcatagcg tacgcgtact
    10561 ttcgtcattt tcttcctctt ctttcaaact agactttgac taacagcaaa aattccgtca
    10621 gttacggctt ctcaaatatc atttatttaa agccactcag cacaaatttt accttttcta
    10681 aggtaaatag taaagccaac tggttgaatt tccttaccag ttggctgaat ttctttttta
    10741 ttttgattgt ttagtctttt tctctgtctc ttttttattg tcgattcctc gtcctgcatg
    10801 tgcaggttta gcaaagatca tgcgtccagc agctgtttga agggcacttg ttacaacgac
    10861 ttcgatacgt tcattcatgt aatgctgtcc atcttctacg acaaccatcg tgccgtcatc
    10921 aaggtaggct actccttgtt ggcgttctgt cccggctttt actactagaa cattcatcgt
    10981 ttcaccaggg atgacgactg gttttacggc atttgccaac gcattgatat taagaacagg
    11041 tacattttgg aattcagaga ctttattcaa gttatagtca ttggttacaa tgacaccatc
    11101 taaaagctta gccaatttga tcaatttgct gtctacttca ctgatatctt caaagtctcc
    11161 atcgtacatt tcgacagata taccctcttc tttttgcaag gcatttagga tatctaatcc
    11221 gcgacgacca cgcacacgtt tcaaactatc ccctgagtcc gcgatgtatt gtagttcgta
    11281 aagaacaaaa ttagggatca tcaaggttcc ttctaagaat ccagtctttg taatatcata
    11341 aatccgaccg tcaatgatca cgcttgtatc caagatttta tatttatgga aattttctcc
    11401 tgattttcgt tctaacagtt gactctcaac ttgactagct gtttcttgct cgattttttt
    11461 gcttcgtgaa ccaaaaactt tcttccattc ttcgattcgt gtcgttccca ttctcaatcc
    11521 cagatagcct aagatgatca tcagtaaaat cggcaataca ctattgacga atggaatttc
    11581 catattgtaa agaggggtcg agatcacaac acctaaagtc aatccgatga tcgcaccgat
    11641 cgcaccgaag agaagataag tcaagctgac ctcacttagt ttttgttcta cttgtttgat
    11701 aatagctgag atatgtttgg ccaacagcaa agataataaa aagaaaataa gtgcgcctaa
    11761 caggctgttg gtaaatgtat tattcagcca tccgtttgac tgttgaccga ttgcctgcca
    11821 agctgagggt aaaaatgaaa tacccaagct tgcgccgaca atgatcatca gcaaagtaat
    11881 gacacgtttt tgcatcgttt ttcctccgtt tcgacaatca tttattagtt agcggaacac
    11941 acgcttcaac gtttcagcca aagttgctac tccaactatc tcgattcctt ttggcgcttc
    12001 ccagttacct aagttgtttt taggtaggta tacttttgtg aagccgagtt tttggacttc
    12061 tttgactcgt tgttcaatat gactgacacg tctgatttct ccagtcagtc caatttcacc
    12121 gatgaaacat tccgttggct gtgttccttt ttctttatag cttgaagcaa tactgacagc
    12181 gatagctaaa tcaatcgctg gttcatttaa ttttacccca cctgcagctt ttaagtaagc
    12241 atcttgattc tgaagaagca acccagctct tttttccagc actgccatga tcagagatac
    12301 ccgattgaaa tcaagacctg ttgttgttcg tttggcattt ccgaacatag taggtgtgac
    12361 taatgcttgg atctctacca aaattggacg tgtcccttcc attgccacaa cgatagcaga
    12421 gccagttgca ccgtctaggc gttcttctaa gaaaatctgt gaaggattag cgacttcttc
    12481 caatccatgc tcatgcattt caaaaatccc aatctcattc gttgagccaa atcggttttt
    12541 tactgctcgc aaaatccgaa aactatgatg ttgttcacct tcaaaatata acaccgtatc
    12601 caccatatgt tctaacattc ttgggccggc gatcgagcct tctttggtaa catgaccgac
    12661 gataaaaatg gctatcccat ttgttttagc gattttgagt agttctgcag tggtttctct
    12721 tacttggctg acacttcctg ccacacttgt gatgtctggc tgcgtcatcg tttggatgga
    12781 atcaatgata acataatcag gggagatatg ttcgatggca cgactgattt cgttcatatc
    12841 tgtttcagcg tatagataaa attctgtgtt gatacttccg aggcgttccg cgcgtaattt
    12901 tatctgctct gcgctttctt cgccggatac atataagact tttccgccta tggctgctaa
    12961 ttgctgagaa acttgtaaca gcaaagtgga tttcccgata cctggatcgc caccaatcaa
    13021 gaccatagac cctggcacca cacctccgcc taacacacgg ttcagttctt ctaattttgt
    13081 tttgatccga ggttcttttt tagggacgac gtctgcgatt tttgttggtt ttgctttttc
    13141 tcctgtcaaa cttgtccgag ttctgcggtc cgttgtatct tgttcgattt cttcgaccat
    13201 cgtattccat tttccgcaat ttgggcagcg tcccaagaac ttaggcgaca catacccgca
    13261 attttggcaa acaaattgta cttttgattt ttttgccatt gtaccgcctc cttttatcct
    13321 aatcatgaga cattaaatca agcttaagta tctttaacgt tttatttact ggaagaacca
    13381 aatccgcctt gacgttgagc agtcgtttga tcttgatctg cttttaaaaa cggcaaaaag
    13441 atcccttggc cgatacgctc gccttttttg atcactacat ccttgaaacc aaagttggtg
    13501 aattggaaca tgatatggcc ttcatttgcc tcattattgt aataatcact gtcaatcaca
    13561 cctactccgt ttgtcaacac taaaaaccgc ttcaacggat tgcttgaacg gttagcaagc
    13621 tgcaaaaatt catcttctcc catgtaagct ttgattccag taggcacgag ggttggtttc
    13681 aattgttttt cattttctga tacatctttt ttcaatccat ctgcgactgt ttccaacccc
    13741 tttttccagc ctgctttcca gatacttgga atcacgacct cttctgctgc ttcaaaatca
    13801 tagccagcag catgatgtgt agcgcgctgc ggtagattga tattttgatc tttatattta
    13861 gaaataactt caaatcctct ttttctcacc ggttttcctc cctaaataaa ctactcctat
    13921 tctaacatat ccgaggctgt aaaagacttt ctttacaaat tcttcaagaa attctgctat
    13981 cttaaagtaa accattaatt cgttcttttt ccaaacgact gcaagttgta caaaaaaact
    14041 attttttaca tgactttact cgtcaaaaca agtagaatag aacgaaatta atgagtggat
    14101 atttgattga ggtgtcacat gcgtaaagaa attaaaaatg caaaacgaat tattgtcaaa
    14161 gtaggaacaa gtacattgat ttatcccaat ggaaatatca atcttgcggc aatcgatcag
    14221 ttagcttttg tcctttctga tcttagaaat cggggaaagg aagtgatctt agtctcatca
    14281 ggagctgtag gcgttggtat gcataaatta catttgtcag aacgtccgac tttgattcct
    14341 gaacagcaag ctgcggcggc tgttggtcaa gccgaactga tgaatatcta taatcaaaga
    14401 ttcagtgtat ataatcaaca gacggcacag atcctcgtga caagagacgt ggtagattat
    14461 cctgaaagtc gtaaaaatgt gaggaataca ttggaacagc ttttgattat ggacacgatc
    14521 cctatcgtga acgaaaacga tactgtagca gtggatgaat tagatcatct gacaaaattt
    14581 ggcgataatg atcagctgtc agccatcatg gcaaaacttg tcgcggcaga ccttttgatc
    14641 gtcctttctg atatcgacgg gttttattca gataatccga ctacaaatcc cgaggctcag
    14701 atgtatcgct atatcgataa aatcgatgaa aagattctgg cacaagcagg tggtgctggt
    14761 agcacatttg gaacaggcgg tatgcagagt aaattaaaag ccgcttctag gatatttgaa
    14821 gcaaacagcg caatgatttt agcaaatgga aaaaatccgg caattatttt tgatattctt
    14881 gcaggaaaag aagtaggtac tttatttaag gaggacttga aatgactgac ttgatgcaat
    14941 taggacaaca ggcaaaagat agcgcgcaac aactagcact tatgcctaca aaccgcaaaa
    15001 atgagctact tgttcaaatg gcacaaacca tcaaaaagaa tcaacaagcg atcattgaag
    15061 caaatcataa agacttagaa aaagcagtcg aaaacaatat cagtgaaacg atgcaagacc
    15121 gcttgcagct gacacctgag cgaatcaatg ccatggctac agagatcgaa aaaattgctt
    15181 cgttagacga tcctattgga aaagtagatg agatgtggac gaacaccgac ggattgagaa
    15241 tcggcaaaaa gcgcgtgcca ctaggcgtga ccggcatcat ttacgaatct cgtccaaatg
    15301 tcacgacaga tgcagctagt ctagcattca aatcagggaa tgccgttatc cttcgcggtg
    15361 gcaaagaagc attcttctcg aatcaattat tagtccaatt attgcagcaa gtactggtca
    15421 gtgctggcga atctccttat gccatccagt ttgtcgatga tacttctcat gaaactgcag
    15481 gacagttgat gcgattgaca gaatacttag atgtgctgat tcctcgaggc ggagcaaaat
    15541 taatccaacg agtcaaagaa caggcaacgg tacctatcat tgaaacaggt acaggaaata
    15601 atcacgtata tatcgataaa gaagcacagt tatctatggc agtgaacatc atcgtcaatg
    15661 caaaagcttc ccgtccatct gtctgtaacg cggcagaaac attgctgatc cattctgaga
    15721 tcgcaccttt tttcttgcca gctattgaaa aagaactggt agaacacggg gtctctttac
    15781 gtgcggatgc tcgtgcgtta gaatatctag agacggctgc tttagcagaa gacacagatt
    15841 gggatactga atatctagat tatatccttg ctgtcaaagt ggtcgattct ttggatgaag
    15901 caattgcaca tatcaatcgg cataatacta aacattcaga aacgatcgtg acagatagtt
    15961 atgcggctag tcaacgtttc ttaaatgaag tggacgcggc ggctgtctat gtcaatgcct
    16021 ccactcgttt tacagacgga tcggtatttg gttttggtgc agagattggt atctctactc
    16081 aaaagttgca tgcccgtggt ccaatggggt taaatgaatt aacttccaca aaatatatca
    16141 tttatggcga tggacaaatc cgcgaataat gtttcgtgtg aaacattatt cccgcctttt
    16201 gccttttttc tgtaaaggtt ctatccaact agaaaggtgt caatatgttt gaaattttaa
    16261 tgattttttt tatttacctt atttctctga atatcgctgc ttttctggga gtcggtattt
    16321 tatccctttt ctttcaattc aaaaaacgaa gcatagggag tcaacgggaa aaatggtcgc
    16381 agtattttga caaaatcggg caaaaagggc tggttacacg cttgcacatt agttacatgg
    16441 ttgcattatg cttattagcg acaatcaatt actattcttt tttcgatcat tcaatcgctt
    16501 atacgattac gctactgatt gctgggattt ttcacttatc ttataaatat cagctgaata
    16561 aaaaccactt gaatcggact ttccgctaaa aaatatccgt acgataagta gagtgccttg
    16621 aagatcggca aaaaactgcc ggacttttta gaggctgtga caaagatttt gtcacagcct
    16681 ctttttccat ataaacggcg ttcgcctacc agctgcctgg taaatctgtt tcctcttcgt
    16741 taagaagcga aaatccgtca aatgtttcgc catcataata acgaagcagc tcatccatgg
    16801 acaagttcat cgttttttct ttttcttctt ccgctgattc agccagtagc tgttcccgat
    16861 acacctgata cataccagct attttttgct gcatcgcttt tctagtttca ccttccgcat
    16921 tgattattgg caattctttg atatagccac gataaatcaa ccgatcatct gaaaattcaa
    16981 tgggtaattc aacgagtgtc aacccgactt cctgaaaata ttcttccagc atctattatc
    17041 cccccattct ttttcttaaa tgaattttag cttacctact gaaaattttc cagtttcatt
    17101 attttgagaa aaaaagggaa tgtgacaaaa atcgtgtcac atcccctttt tccgaataaa
    17161 tggtgttctt ctgccaactc ctcaatcata agtcacaata ttcaacaaac atgagaaact
    17221 gttttttgaa attgttcctt gtgattcaga gttaaacgac agtctcacaa cctcttggta
    17281 aacggcgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagaa
    17341 gctatttttg tttggctgtt cttatgactt gatgcaggac agctttttca caacctctga
    17401 tttcttattc ttcttcattt gtagcaaaat aattacggtg ataaatgata caacatacac
    17461 tgataagaac aatggcacct gcaaaataaa acgcatagct ttcaccaata tgttcaccaa
    17521 tcacacctgc aataaatggt gctaaagctc caccgaaaaa gcggacaaaa ctatagctgg
    17581 acgaagcaac gtttcgttgc agacctggta tttccatagc aatcgtcgtc aacagtgtat
    17641 tgataattcc ttggaaaaat cctgctaaca caatgctgat cggtacaagg actggcatag
    17701 aagttccagc tcctaataac agcaaacaaa taatgaataa gacaaatgct gctaaaattg
    17761 tccgataagt cgtaaacttc cgctcaagag ccggtgcaac gaaaatcgaa gcaatagcca
    17821 gaagcacacc ccagccaaaa aatacaaaac cgacttctaa ttcagaatag ccaatcaata
    17881 aaaatggcgc atacgcaagt aaagtaaaga atccgaaatt atataacaat gcgattagtc
    17941 caactgaacg aagtttatga ttgcttaacg ctttgacacc ttcccaaagg cctacttttc
    18001 tctttggttt ctcagtttca ggtaaaagta ccgctagggc tataaaaccg caaaacatca
    18061 aaaatgctac cccaaaaaat ggataacgcc atgaaccact gcccaagacc ccaccgacta
    18121 gagggccaac agccattccg cagccaagtg ctgcttcata catcatgatt gctttttctg
    18181 tgttaccaac taaaatactg acgatcgttg ctaaagcagt agagacaaat aaagcattcc
    18241 cgacacccca gccggcacgt aacccaatca gcattccgat attcccagat aatccgccaa
    18301 gaccagcgaa tacaacgatg ataaacaatc ctgtcatcaa tgttttttca aaccgatccg
    18361 actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt aactagtaaa
    18421 taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt ttaagattgg
    18481 gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa ctagcgctga
    18541 acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat tctccaatat
    18601 ttccatccct ttctctctat tagcatcata catgatttat tttacatgta aatatgcaca
    18661 tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga ggtaggattt
    18721 ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca cgttctgggt
    18781 atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag atctatacaa
    18841 cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac gagcactctc
    18901 aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa tggatcaaag
    18961 aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat gctttttcga
    19021 cgctcgataa aacaacagct actggactgc tattcactag agaacaacag ctgaacaaaa
    19081 tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa caagaatttg
    19141 gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc caatggtgtc
    19201 agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga acctttctag
    19261 aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg gaaaagaaaa
    19321 agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct agaactgcat
    19381 tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt gttccatcat
    19441 tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat gatcaactgc
    19501 aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga gaataattga
    19561 tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac cattcctaaa
    19621 accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt tactggtagt
    19681 ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat gaaagccacc
    19741 acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg atgcagccat
    19801 aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt aaaaattatt
    19861 ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac gaaaatcatc
    19921 gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt ttatttcata
    19981 aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca aaatcgttcc
    20041 cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa taaaattagt
    20101 cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa tcgtcagacg
    20161 ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct gagcaaaccc
    20221 taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc tcatgacaaa
    20281 acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata cttgaaagct
    20341 tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta ataaggttgt
    20401 cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa actgaaaatc
    20461 accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc ccatgatggg
    20521 atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt tcatctgctg
    20581 cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg cattcttttg
    20641 gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatccg gtctgatttg
    20701 ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt tcttttcttg
    20761 gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc gtagctgagt
    20821 ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa actctcggat
    20881 ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat agcgatcatg
    20941 actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc gatcaatact
    21001 ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat aagaagaaag
    21061 aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct ctgaagtttc
    21121 ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg cttcagcgag
    21181 ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc catgatcggg
    21241 aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat tttctcctat
    21301 gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt ctagctggtt
    21361 gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat gttttacact
    21421 cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa tctgtttgca
    21481 aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta aatcaccaaa
    21541 tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac tccatccatg
    21601 aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa gttcgaaacg
    21661 taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac cacacacgat
    21721 tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa cgtagtgatc
    21781 ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc tattgataaa
    21841 ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa ctgatctgct
    21901 tcgaatccac caattggatt tgtttcatag ccatgatcac gcgcaactaa catgaattgc
    21961 atagccgcta agctagaatc gactttgact acatcgttca tttcttctct tgaaaaactc
    22021 ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg catcttccct
    22081 gattcgtaag cttggttata gatttcttcg ccatattcgt aacattgcat atcgccaaaa
    22141 atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat caaaggacgc
    22201 aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttg catattgaca
    22261 gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg tgggattttg
    22321 tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga aaaatcattt
    22381 ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa gttgggtatt
    22441 caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag ccggggatag
    22501 cacggaacct gtatctttat ggtccatttc acaatgctcg attttcttct gggctttttc
    22561 tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg aaacatagcc
    22621 atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct gcaacttttc
    22681 ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga tctcgtatct
    22741 agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat cggctaattt
    22801 gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct ttctaataat
    22861 tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag tcaaagaatc
    22921 agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa attttttgta
    22981 gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta ttgattctta
    23041 tcaaactata gaagatagct cttctgcagt agaaaatttt acaccgccaa aaatttccga
    23101 aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg cttaaacagt
    23161 aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg tgttcaaaaa
    23221 cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt ttttgtttgg
    23281 ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat aaccataaaa
    23341 tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct tagcactcaa
    23401 ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa ttggcttgga
    23461 accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta aagagttgat
    23521 ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata aacggtagac
    23581 aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc ctacagttgg
    23641 cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat gacggttata
    23701 cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca gattataggt
    23761 ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac gacctgttag
    23821 ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg aatagtagta
    23881 aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca atgattcttt
    23941 gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat tcacatattc
    24001 cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa atagattgta
    24061 attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg cttgagcaat
    24121 catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg aggcaatact
    24181 atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat agccagtata
    24241 gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc catctgtcaa
    24301 ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg atgtactgtc
    24361 ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa ttgtattttc
    24421 tgagacagta gctgactgca aagtactctc tgtatcgttt tcaattcctg tttctgaaga
    24481 actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg ttgtgttggc
    24541 aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt tcatcttttt
    24601 tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag taaaagcaag
    24661 tcaatgtcca tatttaaata acatttgttt ctttttgata aatttgcttt ttctacataa
    24721 aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg tcgtttgaat
    24781 aactatttaa caagtaattc tctttggata ttatccgtta tggattatac ttcctgtgtc
    24841 gtcggacgga taatcatgtt actgacagac atccggtctg gcgtttctat cgcaaatgct
    24901 acagcagaag ctatatcttc ggaggtcaat ccccattctt tttgcgcttc atgaagttca
    24961 agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc tcctggagag
    25021 atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc catgattgca
    25081 cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac atgtcccgcc
    25141 acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg aagtacggcg
    25201 gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca ttcatcacgg
    25261 tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa tacatcgatt
    25321 cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa atctcttaca
    25381 tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc ttttaggcga
    25441 tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag cttcgttgtt
    25501 gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga taaatcattc
    25561 attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct cttatttcat
    25621 cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat tcctgcaacg
    25681 atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat ggcattctcg
    25741 ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt tttcttgatg
    25801 tggattggct ttactgacaa gcctcttcgt tcttttggat cggttagatg gttcaccgtg
    25861 atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc acctaagtca
    25921 acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg tttgccgaaa
    25981 tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact aatgatattt
    26041 tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc tgctaaaggc
    26101 tcttcgtttt cagcttggat ctgatggatc acttcaaaga gcggatccgt tactcgaatc
    26161 tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc ttttatcctt
    26221 actttataac ggacggctta tcatgtctaa tacttataaa acatacttaa ctattccttt
    26281 taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga tattcgtata
    26341 ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc aaaatctctg
    26401 catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga acttggtgtg
    26461 acacttttca ctcggggctc ccgagagata cagttgacgg attctggtcg atatctttat
    26521 tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt aacaaatgac
    26581 gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat ctctttcatt
    26641 tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca tttatatagc
    26701 gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt tggactcgta
    26761 atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca agatcgttgg
    26821 ggacttttga tacatcgaaa tcatccttta gcaaagcaaa ccgtcatcca tccgcaggaa
    26881 ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca actagctgaa
    26941 tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt attgtacaat
    27001 gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg gatcatcaat
    27061 acttatggga cggaaaaaat attcatccca ctcgatccgc cattacaagc ttcactgaat
    27121 ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt agatgtccta
    27181 acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt aacaacgaat
    27241 aagataagca aaagatccaa cgattaaata acataagttt cttaatttat ttcttccttt
    27301 tatttctgat aattccagta aattactata attataaaaa aagaattgac aaaacataac
    27361 tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag atcatactag
    27421 gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc tatttttgtt
    27481 tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag ggtgcgcgca
    27541 tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt ggacaggaaa
    27601 aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg atttttgatc
    27661 gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg caaaatttac
    27721 ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat catgcggaaa
    27781 ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac cacattgctt
    27841 tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta agagaaatcg
    27901 aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat ctgacaaaag
    27961 agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat attcacagcg
    28021 gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt gtatctgaca
    28081 tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca ttttcactga
    28141 attattcaag actggtgaat catttacgac tgcttctaca acgaacccat gcgcaacaat
    28201 atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca gaaagctata
    28261 agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg atcacgaaag
    28321 aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc gtacaaacaa
    28381 atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa tgggacgttc
    28441 ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga tcggaaactg
    28501 gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg gtaacgcagt
    28561 tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt cagtggacaa
    28621 atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa atgttttaaa
    28681 aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc catcttttgc
    28741 tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag ctctttataa
    28801 tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca agcgtctagt
    28861 gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc tcgtatggcc
    28921 tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag gctggggtgg
    28981 tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc atcaagcaat
    29041 gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat ttttggcttc
    29101 tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa tcatgatgtt
    29161 cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa acaaagcaaa
    29221 aacagcttcc ttgatgctag cagcagcttt tgcttcattc tttactggtg tgacagaacc
    29281 acttgaattt tcatttatgt tcgttgcttg gccattatat gtcttgcatg caattttcac
    29341 tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg cctttagtgc
    29401 cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc cattgatgtt
    29461 gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact ttgctatcaa
    29521 aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg acgatactgc
    29581 aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg ctgcattagg
    29641 cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt tgcaagtcaa
    29701 agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg gtatgaagat
    29761 catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg ttgcagatga
    29821 gatggcaaaa ctacatggtg gtgctgcagc acgacctgca caaacaaaca ccgtggttaa
    29881 aacagaaaca tttgccggtg aaacgatcga acaagatatt tacgctatcg caaatgggaa
    29941 actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga tgggtgacgg
    30001 ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta cgatcacaaa
    30061 cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg aagtcttgct
    30121 tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat atgtagaaga
    30181 aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg ctattcaatc
    30241 tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag tccaaagctt
    30301 agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg tttccaacaa
    30361 agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga gctgagacaa
    30421 aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca actccgcggt
    30481 aataagtcca gctaaacgaa acatatgaag agctattttt tatttgactc ttcttattac
    30541 ttgatgcaga acagcttttt cacaaaattt ttcttcatac tcaaaactaa tcggcttttt
    30601 cacaacctct ttatacatta ttcctgatcc aatatttctt ttaatgctgc ttcaagagaa
    30661 gttaatggtt ttcccaatac tttttctaaa tcatcagagg aaatagctaa ctgatccttt
    30721 ttaatatcat gttggatcgc taaaaacatg tcagctgctt cttgagggaa tccggcttct
    30781 tttagatttt cgacaaacgc tgcatcactt gcttcaataa tttctaactc tttacctgtc
    30841 gcacgctcta atgccttggc taattcttca tagtcagtta gattgccgga taattctaag
    30901 atttctggag agtcactttc tactagtact tttgctgcag cttctgcata ttcacgtttc
    30961 aatgcccaac ctgtttttcc atttcctgca gcataaacga attgaccagt ttttaaagcc
    31021 tgcccaatga taggtaattc attctctaca taccaattat tccgtaaaaa tgtatgggaa
    31081 atacctgaat ccttaatcat tttttcagtg aattggtgat ctggcgctaa tacactgatc
    31141 gagtgatcgg catctgcaaa acttgtataa gcgatataag ataccccagc tgcttgtgct
    31201 gccttcacta cattcttatg ttcggcttga cgatttcctg gaacgccaga gacaaaaagc
    31261 aatcgatcta ttcctttcaa cgcttctttc attgagtcta aatcatcata atcaccaata
    31321 cgtatattaa atccagcttc tttcagtgct tctccttttt cttcactacg tactagtaca
    31381 tagatttcat tttgtggaac catctttttt aagaattcca gcgcataacc accaaaacct
    31441 ccagttgctc ctgttactaa ataatgcata tttgccactc cctattcttt ttatttgtta
    31501 agatttacag ctgtacattt ataaattaca cctttatcat aaagagtgtg gtaagataag
    31561 tcaagcaaaa aaaatgaaag gatgacaact gatgaagtat tctctgcaat ttagtgatgc
    31621 aatccatgta cttgcctata ttgaaatgtt tcaagatacc cgatcacttt ctagtgagat
    31681 gattgccagt agtatcgaaa caaatgcagc aaatgtacgt aaaatcatga gtcagctcaa
    31741 aaaatccggt ttgatcttta caaaaaccgg aaaaccgcag ccttcccttg ccaaaagtcc
    31801 agatcagatt tctttactag atgtctatcg cagcatcgaa ggtaatacac agcttatcca
    31861 agtcgatcca aaaacaaatc caaattgcgt cgttggtgcg aatatccaag aagctttaga
    31921 agaaagctac gctcggctgc agaaaaaggc agaagaagaa atggctaaga tcacacttga
    31981 tagtctgata cataaaatag ccgttttgga aaaagaaaaa agaccagaaa acacaggcct
    32041 cgtagagaga tttttataat tctctctact gtgcctgcat ttttctgatc ggaatcgtct
    32101 acataaattc ttttattctc cgggggacaa aagccttgtc atgccccttt ttcctctcta
    32161 atgctcaggt caaagcgctg aagtcatgac gtcttgataa acggtgttca agtagctgac
    32221 cctcggcatt agttctgaaa aatgcaaaat atggaaagct atttcccatt tttcctctct
    32281 aatgctcagg tcaaagcgct actttcacga cctctatatt atcatggttt attgactacc
    32341 ggtatccaga tttctcccat atatttcccg ttggtaactc ccatttcgac tacagcattt
    32401 ggacccccaa cataggcgta atcttctact tctcttaaaa catgcccaaa ggccgtattc
    32461 gtcaaatcat ttcttaattc ttcaggcgat tctgctaaag cgcttacaac aagatacgtt
    32521 ccttttggga atgggatgat tcgcgtagtg tttggaacat cctgactcgt ttgaacaccg
    32581 acatagtaca tcattttccc gttatagggt tcgttgactg taaagaaata atgattttca
    32641 gcagcttgtt tcaatgcatc tattgttcca ttaccgataa ctttttgctc aaatgcttgt
    32701 ttctctgcag ccaaaccgac aaaatccgta ttgctactct tcaattccac accaataccc
    32761 gtcaccgtaa aatctggttt ttcttcgatc ctatactttg ccatttctcc caccctttct
    32821 attatattaa gttcatctta cataaaatta aagggaaatg acacgattac gctctttttt
    32881 ctagggatat ttttattttt taactatttt tttgctttca ctgcaatcca aatctcactg
    32941 taaacattac tgaaatcctc tccccatttt gtaaaagaga tctcgggagc ttcgactaac
    33001 ccataatctg aagaaggaag ccattctgca tagattttcc cccaagtatc ttgtaaagtt
    33061 tggggaaatg gccctttatt aggaaaaatg gcccaagtgt gttcttctat agcaagcatt
    33121 tctaaatcag cgtacggatt ttcttttgtc gtagcaaagc caatcatgtg catcaaactg
    33181 cccttttctt ccaagcggtc tccatcaaat ccataagaaa cgttgattac ttgatttgga
    33241 tataaatctc ctaacttatg catctcttcc ctttgagtct ccgtgatcgt ctgcgctaac
    33301 ttttgtattt cttgattgac tccttcaaat ttgacaggaa cttttttcgc cacacctgcc
    33361 acataaaact gttctttctt ttcgatacga aattccatcg attttcctcc tctgacatca
    33421 ataaaaaatg tcaagcgtgg aaaagatttt tgtatgccgt atctactgat ttctgaaggc
    33481 agatatcctg accaatcacg aaaagctctt gaaaaacctt cgactgtttc atatccatat
    33541 ctaaacgccg tttctgtcac tcttgatcca tctagaagat cgcgattagc caaagccaat
    33601 ctacggtttt tgatatagtc cattagcgac atccctgcaa taaacgtaaa gatgcgtttt
    33661 aattgatatt cagagattcc tgtattttcg gctattattt tataggaaag tccttctgtc
    33721 aaatgttctt cgatgtaatc gatcaattgg ttcagatctt taagcatatt tatcttcctc
    33781 cttgattgcc tttttatcat attatcaaag aaagtcaatt acttgatgaa tcaggaacca
    33841 atttagtcag atgtagaaaa tgttgtatgg atctaaagaa aattctcaag cagcgtttcg
    33901 taacggataa ttggtattat ctgcctatct atgttttacc tttagaaaaa taaattttgg
    33961 ggaagacaga ttctaagtaa acaaagcggt aaaaatatct tttattccct tagttaatcc
    34021 gcttactacc tactgtacaa agcaaattgt tgttcaaact gctgatcaga acgctgcttg
    34081 gatttttctt tgcgatacat catacctctt ttatcaattg gaacaattat ttatttctaa
    34141 caaaatcact tcatttttgt cagataaata aaaaacaaaa aaagttccat ctttaaaaca
    34201 cgctattctt ttactttttc tcacattctc taagaaaaga aaaggagaat gaatttttga
    34261 caattcgagc aaaagtgatt atcaaatatg taaaatcagc tacttttttc ttaaacgttt
    34321 ctttatacta aatataaata agtgtttcat gaaaggagaa ctagatgaat aattggcttg
    34381 agtattttcc tgaaaacgtt ttggaacgcg gttacagcta tcatctgcac ggttttgtcc
    34441 gtcatttgaa ctatacaagc aagtacttgt ccgcaacagt ttctggcacg gaagattata
    34501 aggtagtgat cacttgggat gaaaagacca acatgacttg tgattgtcta tatgccatag
    34561 aaggaaaaaa atgcaaacac atggctgctg tcttattcgc ttacgaagaa cgtccaataa
    34621 aaaaatccaa ctactcactt tccgaactct cgtcccttgt ttcctccgct agttcctctc
    34681 ttgtacggga gcttttaaca gagattctta tagaacatcc gcactttatc gaacgcttca
    34741 aagttaagat gccatttcat gcaataaatt attcagataa attaacaact atcatccata
    34801 aatacgatca tattatcaag aaaaacaaaa acaggaaaac agcgaagttc atcatggaga
    34861 tgcgaaagtt catccaagaa gcagttgaat cgcttattca gcaaaatgcc tatcttcccg
    34921 cattcgaatt gatcaatgaa gtgattgcta ctctcgaaac attctattgg gagcctgagg
    34981 atgaacgaac ccttcttctg atagaggact gttattattt atggaaagaa ttattagcag
    35041 aagcacctca tgcagaaaaa aggcaaatgt tctcttggtt cgtctgccaa gtcgatcata
    35101 ctgacgcctc ttattcaaaa cgctactcta taaaaatcct gaaagaagac tttcgggaaa
    35161 aagaattttc taaccaaaag aaaaaaatag ataaaaaact aaagaaacgg taaagaaaga
    35221 cgatttcaat gaaaaatagc aagtaaacga taacaaccat cctaaatcca caagaaatac
    35281 agcagacctt gctttaaaaa cacgaatcat gcaaaggatg aagtaaagaa gacttaacgc
    35341 gtacccaata ttagtcattt gactattagg tacgcgtttt gttttataag tcatgacaaa
    35401 tttccttcgt tgctggatag attttccggc attgttgata gattttatga tacttttcta
    35461 cattttgtaa gagaggttcc gctgcatctg tatattggat aaaattctga caacaacttt
    35521 caggtgtgtc gaaccaccct aaccctagtg cagcaatcat tgttgcgcct aatgcaggtc
    35581 cttcctccat acttaaacaa tagaccgttg catcgaaaat atcagcttgg atttgtagcc
    35641 atgcaagatt tttcgcgcct cctcccaccg aaacaatacg tgtaaatctg cgacccgcta
    35701 gttctttacg tttcaatccc accattgaca aatgctcatt aaaaatttag aatattcaca
    35761 agtttaacga caattatgaa aatttatgtc gtgctaaagg agttttgtta ttggatgaaa
    35821 agttagatat aaaaactgcg atcaaagaca cgttacctac cgttttcggt tatatcggta
    35881 ttggacttgc atttggtatc gttgggaaag ctgccggatt tcatccacta gtcgtcacgt
    35941 tgatgtccct gctggtctat gctggttctg cccaatttat cacagtcagc atgcttgcta
    36001 gtcacagccc attgctttcc atcgttttct cgacctttct agtcaattcc cgaatgattc
    36061 tgatgagtat gacgattgct ccttatttca agaaaaatcg gctgcttcaa aatctgctga
    36121 ttggcacatt gctgacagat gaaagtttcg cactgggaat gaataaattg aactatactg
    36181 gacaaaagtt aaactttcgt tggatgaata cagctaattg gatttcttat ttgacgtggg
    36241 tgggttcttc tcttgttggc gcacttttag ggaattttat tactgatccg aaaaagtttg
    36301 gtttggattt cgccatcgtc gctatgttta tcgggttact ttatcttcaa gtcatttctg
    36361 atagaaacac ctctaaacga ttacagttga tcctgattgg tctcactttg attctggtat
    36421 atgtcggact gatttttatc ccaagcaact tagtgattgt agttgtaaca ctgattgggt
    36481 gcggattggg ggtgtggatc aaacatgcct tcttttgagt ttacattgct gactatttta
    36541 ggttgtactt tagcaacttg gctttctagg gtcctaccat ttgtcttact gaaaaaattc
    36601 gatttaccgc aaccgttgct tgagtattta agttttgtcc cgatcgtcat tatgtctgct
    36661 ttatggttca gcagtctatt tacccaaaat atcggacatc tgccgcagat caatctcgaa
    36721 aatgctttgg cttcagttcc tacactttta gctgctattc tttcaaaaag cttattggtg
    36781 atcgtcttgg caggaattct ttccctttcg ttgattcgct tattataaat aaaaaaacaa
    36841 gagcttatgg caaattactg tcatgcgctc ttgtttttat caaagtcaaa taagaaaaaa
    36901 atcggcgtat tcttgcttat ccgttgaacg gaccactaac aacgactttt cctaacatat
    36961 gtcctgtttc tacgtctttg gttgcttgtt tcaagttctt cgcattcatc ccatctgaat
    37021 agaccttgct caatgtactg cggataatcc cttcatttgc taaagcagca atgtgcgcta
    37081 aagcttctcc ttgcgtttcg atttcatagt tatagtctgt tttagcgaac atatactccc
    37141 agtcgaagct gatagattgg tttttccaaa cacctatatc taacggctct ttgatcccta
    37201 cgatcgttcc cacatgtcca aatggtcgga caaggtgctt gatttgatca aagtagcggg
    37261 taatatcgaa taatacagcg acatactcta cttgatcgat tcctaattca cgaaaacttt
    37321 tacttaagtc gtgatgataa tcgattggat agtctacacc tgtttttttc agccattcaa
    37381 aattcttcgg acttgcagtc gcgtagactt ctaggccagc ccaatgcgct agttgattca
    37441 aaatcgaacc gacgcccccg gatccgttga tcactaagat ttttttcccg cgattggcat
    37501 tttcttctgg agttagacca aatttttcaa ataacaattc ataagcggtc aatgaggtca
    37561 acggcaaggc tgcagcttct tcatctgaaa gattttttgg tgcaagagcg acaatacgtt
    37621 cgtcaactag ttggtattct tgattgcttc ctgctcttgt tgttgtacct gcgtaaaaaa
    37681 cgcgatcgcc gacagaaaat ttttgcactt cgtcacctac agcaacaact ttcccgactc
    37741 catcaaaacc taagatacgt aaagcatttc ttatcccgtt atcttggcga agtttcgtat
    37801 cgacaggatt aacagatact gcgttgactt ttaccagaag atcccgtgaa ctgggtacag
    37861 gtatctgttc tttttcatca ataaagcttt ttggatcatc gatcggcaac ccttcataaa
    37921 aaccgactac cttcattaat tccttttctg acatacgaat tcctccttag cttcatcaag
    37981 ttggagcatc catacagtgt tcatttcaat atatatagta tgctatttgt tccacctttt
    38041 ttattcatca taattattct cttacttaaa gtaaactatt tgcacttttt tggttaaaaa
    38101 agaggttgcg acaagacttt tgtcacaacc tcttataaac ggtgttctcc taccaactct
    38161 tcggtcacaa gtcaaaatat tcatcaaaca tgagaagctg ttttttgaaa ttgctccttg
    38221 tgactcagag ttaaacggca ggctcacaac ctctgatata aacggtgttc aatcaactga
    38281 ccctcggtat tagctcaaaa aacgacaaaa tatgaagagc tatttttgtt tggctgttct
    38341 tattacttga tacaggacag ctttttcacg acctctaatt cacggtgttc aagcaagaaa
    38401 tcattgttca ctgaaaagcg ttggatgttc tttttcaaag gtctctaaga tctctcgtac
    38461 ttcatcagta gatttcgtat tcatcaactg aactcgcaag tcatttgctc ctggaaatcc
    38521 tttgatatag attttaaaga aacgatgtag tcctgtgatg gaacgcggga tgatttctga
    38581 ataatgatct tgcagatcta gttgcagacg aagtagtccg atcagttctt tttctgtatg
    38641 ttccctcggg tctttttcaa atgcataagg attcttgaaa atccctcggc caatcatgat
    38701 gccatcaacg ccatatttct cggccaattc ttctcctgtc tggcgatcgg ggatatctcc
    38761 attgatcgtg attagtgttt gtggagcgat ttggtcacgt aaagcaatga ttttaggaat
    38821 gacttcccaa tgtgcatcta ctttactcat ctctttacgc gtacgcaaat ggacagaaag
    38881 attggctata tcttgatgaa gtaggtgact gatccattct tccatctcag acatttccgt
    38941 atagccgata cgcgtcttga cgctgacagg caaaccgcca gctttagctg cttcaatcaa
    39001 ttgtgccgct acttctggac gaaggatcaa gccactgcct tttccacgtt ctgctacatt
    39061 agggactggg cagcccatgt tgatatcgat tccttggaag cccatttctg ccatagcaat
    39121 gctcatttct ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca tcggctgttc
    39181 atcttctgta aatgttaatc ttcctcgtac actgtctttt ccatctggat gacagaaact
    39241 gtctgagttt gtaaactcag taaagaagac atccggtgct gctgcatgct ttacgacatg
    39301 gcgaaataca acatctgtta catcttccat tggtgctaaa acaaaaaaag gctttggcag
    39361 ctctttccaa aaattagtag tcatttattt gttactctcc attctatcaa gcagtgaagt
    39421 cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa gcaaagtgaa
    39481 cagcttaaaa gatgtttctg attgagcaaa aaaaagactg tgacaaaaat cttatcacag
    39541 tccattttcc ggaataaatg gtgttcaaaa gcttgcattt gcggtaataa gtccaaccaa
    39601 acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg cagaacagct
    39661 ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat tcgctcaaaa
    39721 aacaacaaaa tatgaggaac tattttcccg tttgtttctc taatactcag gtcaaaacgc
    39781 tgatttcaca acctcttttc taacttctca atcctcttcc tttattgatc aaataccaag
    39841 taacggcgta caaaacaacg atgaataaca caagtacgat catcgatgtt gctactggca
    39901 catcgatcgt tccaaggaat ccgtaacgga aaccggagat catataaacg atcggattga
    39961 cttttgaaac agcttgccaa aagggcggta acatagagat ggcatagaaa acgccaccta
    40021 gataagttaa cggctgcaag acaaatgtag gcacgatcga tacatcatca taagactgag
    40081 caaaaatccc gttgagcagt cctgctaatg aaaacaagat tgccgtcatc aataatgtaa
    40141 tgatgaccat gaaccatgaa tacacatgaa gtggtacaaa gaacaaggaa atgatcgtta
    40201 cgagagagcc gacgagaata ctgcgaccca caccaccaat cacaaagccc caaataatca
    40261 catgcgtagg aacaggtgct accaagattt cttcgatatt tttctgaaat ttttgagaga
    40321 agaaggacga agaaacattg gcatacgaac tagtgatagc agacatcata atcaaaccag
    40381 gaacgatgaa ttccatatat gaaaaaccac ccatgtcacc gatccgccca ccgatcattt
    40441 ttccgaaaat aacaaaatac aaggatgtcg tgatcactgg cggaaccagt gtttggaccc
    40501 atatacgcaa atagcggttc gtttccttga tagctaaact ttttaatgca gtaaaataaa
    40561 gattaaacat ttttctcctc cgtatgatta ttttcttctg tgattttcaa gaacagttct
    40621 tctaaacgat tcgacttatt cctcatagaa agaactttga ttccttgtct tgtaagctga
    40681 tcgaagattc cgttcacccc ttgatttcgt tcaacttcga cagcgattgt ctgctcatct
    40741 tcaaatgtac aagcatatcc ttcaatcaca ggtttggtat catatggcgc taaatcaaag
    40801 ataaatgttt caaattgcag ttttgacaga agctctttca tacttgtatt ctcgatcagt
    40861 tcacccgatt ggatgattcc gatgttgcga caaagcattt cagcttcttc tagataatgc
    40921 gtcgtcaaaa tgatcgttgt accattttcg ttcaattctc gtagaaactc ccacatctcg
    40981 cgtctcaact cgatgtcaac tcctgctgtt ggttcatcca agatcagcaa acgtggttca
    41041 tgcatcaatg ccctagcaat catcagtctt cgcttcattc ccccggaaag catacgtgca
    41101 cggacatttc tcttttccca aagattggac tgcttcaaat atttttcgct gcgtttcatc
    41161 gcttctttgc gagagacacc gtagtagcct gcttgattca cgacgatttg ctggaccgtt
    41221 tcaaaagggt taaaattgaa ctcttgtgga accaatccaa tctgttgctt tgctcgaact
    41281 agatccttgt ccagatcata accgaagaca ttcacttttc cagatgtttt attgactaaa
    41341 gaagtaataa tcccgatcgt tgtagacttc ccagctccat ttggccctaa tagcgcatag
    41401 aaatctcctt ctttcaccga aagatcgatt ccccgcaatg cttcgacccc acttccatag
    41461 atctttttca aattatttat ttcaagcgca tttgccattt atttcctatc tccttataga
    41521 catgattctt aattgtatca tggaatacag cagatgaaaa aacaacttgg cttccacaca
    41581 aaaacacacc tgctaacaaa gaggagatgt gttttctagt tcatgatcat cttcgataga
    41641 ataactatct aaacaactct ggaactttta aaatcataaa agtaccgaca gcaactacta
    41701 cgatgacata tagaagatcc ttataactga ggttcttgtc ttttttattc atgttaagct
    41761 ttcctccgtc cttttatcga aagctttttt cttttatttt aaccgaaata aataaaatgg
    41821 aaatagttat tttcaatttc tgttttcgct gtttgtttta cggagaactt tgccatggaa
    41881 cgtgcgtctc gtcgctgatg ctaagttttt tcgataaaag ctgctagaag gaagacagcg
    41941 tggtttattt tgtatcgtct tttgctaaat cttccaagat catctggaca gcagactcgt
    42001 tcccattgaa ttctgccact tttttggcat caatgtactt cttttgataa tcgtcttttt
    42061 ggacccacgc atcatgcttc gtttgataaa gttaatcaaa cggataccac tgacgctgtt
    42121 catcgaacaa caaactttct ttttgggcct gttcaactat ggatttagga taattcaaaa
    42181 ctttcagcaa ggcaatcgca tttcgggtcc ttgaaggtcc ttcttttaat ttgaaatcaa
    42241 aactgattcc ttttccttct gttacttgtt cttcaaagtg gacattctca caataattct
    42301 ttaaaatctc tgtcaattcg atatcatgtg tggcgacaaa tgctaaagat ggatactccg
    42361 caagccaccg aacgactgaa gcggaagaag caatccgttc gatcgtattt gttcctttta
    42421 aaatctcatc tacgaagcag tagcagcgtt ctttggaagc cacttggtcc agcaaacgtt
    42481 tgatcgattt gatttctgaa acaaaataac tgtcgccttc aaataaatca tcttcgattg
    42541 ccatggaagt caacacatgt ccaggttgca gggtaaactg ttctgcaatc gctgtttgga
    42601 tcgtctgtgc tagaatacag ctgatggcta tacttttgac atacgtagat tttcctgatg
    42661 cattggaacc cgtgactaat gtattcttct gccagttgac tggattaact accgcttctt
    42721 ttaacaatgg atgatagata tctttcgctg tcacaccgcc ctcagtaaac tctggtcggc
    42781 aagtgatcgg catataggtg cgataattca aaacggctgc tgcaacttcc aattctccca
    42841 gcaattccca cagcttgatg gcttcagttg aatgcttttc taatttagtg agaacgaaat
    42901 gataggagat aaatggcagc atgaacatga tgttgacata ttcaaaaatc aattctgctt
    42961 cactcccgct cttcattcga aatgaaaaac ctaatttagg gatgtctctt aatggtttag
    43021 tttgctgttt cagctctttt tgaagtggtg tcgtcatttt tgctaactga ttggcggctg
    43081 aaatcgttga aaccaaataa ctcatgctat tcagctctgt ttctagtgtt tgtttcttca
    43141 ctagataata gaccgtattg aatactaagc caccgattgc tagatagatt cctagtactt
    43201 gccctaaaag aagcaaacct acacccacca gcggaagcaa gccaagaaaa ataaataaac
    43261 ctacgcgtcc aatcttttta ctttttccat ccgctagata ttgttttgca aaattatgat
    43321 ctttttttcc taaacgagcg aattgatatt ggattctttc tctcagttga ggattttcct
    43381 gataaaaagc aattagtttt tctaactgat aatcttctcc aaaatcaaag ctacgtaaac
    43441 gctgatacaa cgcttctgaa cctacactag aataggtact gttgatccct tcaaatactt
    43501 cgaacatatc caagtcatac caagtaagat cgtcgatttc actatcccat gaacggaatt
    43561 ttttctccgt gagccacgca tccttcaaac tttcttcttt atcaaatctc ggttgataag
    43621 gaatttttcc ccaattacta caaactgttt cctttacttt caaacgtgaa aaaatgtcta
    43681 aaacaactaa taaaataaca actgcaataa taattaaaac gataaattct ctttggccca
    43741 tatccctctc cgttccttga tttgttagtt ttagtatagc actgttgcgc tatttgaaaa
    43801 gggggtacaa aaataactgt cttcgttacc taattcgtat caatcacacc aaaataaaaa
    43861 caccttccaa aaaagaagat gtctttaaaa cgagatacaa ttttttgtca ttcacttcag
    43921 gtaaattaga gccaattaaa ccctccgcaa cttgacggag atggagaaga tgcccccgtc
    43981 taatctagcg gtcatctctc ctcccattcc ttccaccaat ttttttgaca ggtaaagccc
    44041 gattccgcta gattccgtat ttgtgcggct ggtattttcg gtataaaaac gctggaagac
    44101 tttttcaaca gaggtttgcg ctcctggttt gataggattg gccactgtta atcgaatgaa
    44161 tgaatcctct tttgaaaatg tcacctttgc ttgtccatct gcataacgca ataaattctg
    44221 tatgatattt tgcagcactc gcagcaacat gagcggatct gctaatacag ttgtatttat
    44281 cgagtgttca gcgaacgcta cctcgatatt tttctcagaa aattgttcat aaaaagagaa
    44341 aaatacttct tccacctctt tagaaaaaga aagcgtatgg agatccattt ctttttgctt
    44401 cgtctctatt cgagcaagat cataaaactg atccgtcata ctgagtaatc ttgcaacaga
    44461 ttgtttgatg atcgttaaat acttctcccg tttctccgta tcctctacct gttccagtaa
    44521 ttgtacataa ccgttaatag cagtcaaagg agttctgaga tcatgagaaa ggccgacgat
    44581 cgatagtctg atgttttctt ccatttcttg aaatgcttgt cctttttgtt cattttcatc
    44641 cacccatgta ttcagtgcat ctactaggtc cattagattc ttttctcgaa attcgactga
    44701 aagacgacca ccatattctt gtcgttcaga aagagtggcg attttcttag ttaatctctt
    44761 gatttccctt tgcaggaaga aaataaaact tcccataaag ataacaacga gaagcaacag
    44821 ccacttaatc aaatctaaac cccactcccc acaccgtatg aatatacaca gcatgtgcct
    44881 ttgctagttt ccctcgaaga ttggaaatat gcatattcac tgttttctca ctatcaaaat
    44941 atggttcttg ccaaactgtt tcataaagat ttgctcgcga aaatactttt ttcggatgtt
    45001 ctaaaaaaag cttcagcaga tcgaattctc ttcctgtcaa atggacagcc acaccattca
    45061 catttacatc cctcgactct ttgtccagta agatttcctt gtaatagatg cgttgcgaca
    45121 gatcttttac ttggacagaa gtctgatgac gaagttggat gccgattcgt aacaacagtt
    45181 cttcgatatc aaatggtttc gtcacatagt catccgctcc tgctgccaac aaggaaactt
    45241 tatctctttg atcgcttttt gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga
    45301 tttctgctaa caatgcttca cctgaaagcc ctggaagcat gagatctaga aggatcaaat
    45361 cgacaggata tgtgcttaac aataattttc cttccgtacc tgaataagca tcaagaagtt
    45421 tgtatctctg ttcaagaacc tcctccagta aagaatgaac agcttcatcg tcttcgataa
    45481 tcaaaatagt ttccagcagg aacacctact taatgtcaga tttttggaaa ataaacattc
    45541 ctagagctga tgataccacg attgttacta gactgaccag aatcattttc ggtgcttgcg
    45601 aataagttgc ttgttgcgca aagtccgtat acatgagcca cgtttccgta aaccactccg
    45661 ttagaaattc caagcctttg atttttgcga aaacaccggg aacgaaggcc gttcccatga
    45721 tataaagaaa agaaagaagc aaactgacac tggtacttct agtgatgaat gtcaaagcca
    45781 ctggaacaga taatgtcgct aatatcagca gaagcaatac aggaaatact tttagaacat
    45841 aatttccata gtttttgatt ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa
    45901 tcatggctag ccccgtaatc aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa
    45961 gtttcgacac ataataagtc attcgattcc gtcctaaaga taacgtattg cgaatcgtac
    46021 cgttttgaaa ttcttcccca atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc
    46081 atgcagccat tttttccacc attaaaatcc ctgttgcacc gttgatgact gtatccgttg
    46141 cttggtttaa ttcattgccg tattttgtat gcatatacca ttcgttccaa gaataaaaaa
    46201 gcggaagtac gaccatcagt cctagtgaga cccacatttt acggctcttc catactttta
    46261 ttttttcagc agttaataga ttaaacatgt ggattgcctc cagttaattg taagaaataa
    46321 tcttccagtt tttgcccaac taggtggata ccatccacag gaacttttgc ctctaccaat
    46381 gcttgattga tggctgctac ttggtcaatt ccttcaaaaa gctggataac acggctagat
    46441 tggacaaaat aatccctata accaagagag tcgagaaccg ttactgcttg ttccgtcgcc
    46501 gaagtgtcaa tttttataaa ttgacgagat tcttgtgcca attcttcttt cgataattct
    46561 ttgatcaagt gtccttcatg caaaataccg tagtgagtgg cgatctgtga caattcatct
    46621 agcaagtggc tagaaagcaa gacggtgatg ccccgttctt ttaccaaacg ttggatgatt
    46681 tctcgcattt ctgccatccc tgctggatcc atcccattcg tcggttcatc caaaatcaaa
    46741 aattctggat tcgtgatcag tgcatttgca atagctaaac gctggcgcat acctaaagag
    46801 aaattctttg ctttcttttt ccctgtattt tccaatcgca tcattttcag caggtcttcg
    46861 atttctctat ccgacacacc gccgttcgct gcttggatac gtaggttatc tcttgcagtt
    46921 aattctggat aaagagcagg ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc
    46981 aaatccgtcg ttccaaataa ttcgatcgaa ccttcatcaa tcgagatcaa acccataata
    47041 gttctcataa aggtagactt acccgctcca ttctccccaa tcaaaccata gatcattcca
    47101 cgtttgattt cgatcgatac tttatctaat gctttggcag caccgtattt tttggttata
    47161 ccagttgctt tcaagaccgt ttcatttcca aaatccatac ttatccttct ttcttattga
    47221 taagtgaagt atagttgacg ggtataaaga aagcagccag aaatgtataa agaaagtgta
    47281 aattttccaa ttgaatttca agaaaaatca gactgtctcg cagacgtggt tgagttgtct
    47341 gccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    47401 ttgttatcgt tgacatcaga cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    47461 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    47521 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    47581 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    47641 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    47701 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    47761 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    47821 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    47881 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    47941 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    48001 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    48061 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    48121 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    48181 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    48241 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    48301 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    48361 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    48421 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    48481 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    48541 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    48601 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    48661 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    48721 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    48781 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    48841 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    48901 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    48961 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    49021 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    49081 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    49141 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    49201 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    49261 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    49321 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    49381 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    49441 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    49501 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    49561 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    49621 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagccctt gttaccagta
    49681 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    49741 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    49801 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    49861 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    49921 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    49981 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    50041 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    50101 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    50161 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    50221 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    50281 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    50341 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    50401 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    50461 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    50521 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    50581 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    50641 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    50701 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    50761 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    50821 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    50881 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    50941 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    51001 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    51061 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    51121 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    51181 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    51241 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    51301 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    51361 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    51421 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    51481 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    51541 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    51601 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    51661 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    51721 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    51781 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    51841 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    51901 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    51961 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    52021 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    52081 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    52141 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    52201 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    52261 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    52321 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    52381 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    52441 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    52501 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    52561 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    52621 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    52681 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    52741 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    52801 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    52861 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    52921 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    52981 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    53041 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    53101 tagtagccga accagagaga ctatcagtta cttccccaaa atctattatc gaatgaaaga
    53161 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    53221 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    53281 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    53341 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    53401 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    53461 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    53521 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    53581 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    53641 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    53701 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    53761 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    53821 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    53881 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    53941 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    54001 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    54061 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    54121 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    54181 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    54241 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    54301 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    54361 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    54421 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    54481 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    54541 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    54601 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    54661 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    54721 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    54781 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    54841 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    54901 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    54961 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaataatgt atgagccaag
    55021 agcaaaatgt tcttggcttt tttctttgca atgaatgagg taatgtatga gatttttaga
    55081 tttattcgcc ggtattggtg gctttcgttt aggtatggaa caggcgagcc atcattgtat
    55141 cggtttttgt gaaattgata aatttgctag acgtagttat aaagcaattc atgatacaag
    55201 taaggaggtg gaaatgcatg acatcacaag tgtatcagat gagtttattc aatccctcgg
    55261 accagtggac atcctttgcg gcggatttcc gtgccaagct ttttcaattg cgggaaagcg
    55321 gcaaagattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcaccactct
    55381 tctccgacct aagcttttat tccttgagaa cgtccgagga ttgctcaatc acgaaggagg
    55441 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgacg tggagtggta
    55501 ggtgcttaac tcaaaggcct acattcccca aagccgggag cggatattcc ttatcggaca
    55561 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt caacatctga
    55621 tcaaaacatc agaaatttgg taaatatcaa tccttccaat cgaggaatgg gtggacaagt
    55681 ctatggttca gatggcgtag cgccaacctt aacaggtgat gaaggaatca aaatcgcact
    55741 gccagcaagg gatggaatct ctgtagcagg actattaccg ggaaagtttg aacaaggtaa
    55801 tcgagtttat gaagttactg gaaccgcccc caccttgtca acgaaacaag gtggtacaaa
    55861 aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg aagccagaag acagtgtgaa
    55921 tctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac
    55981 cttactaaca ggagaccaac aagcgatcgt cacggatcag tatcaaattc ggaagctaac
    56041 acctagagag tgttggcgac ttcagggctt tcccgattgg gcttttgatc gagattctca
    56101 agtcaattca gatagtcagt tgtataagca agccggaaac tctgtaacag ttccggtcat
    56161 ttttgatatt gccagaagat taaaagagtt ggatgtacat ggtttatgat gagttgattg
    56221 gagaaattta ttgggtgatt ggaaagattc agtcagaccc agaattagaa gaacaattgc
    56281 gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa
    56341 tgaatgagga gacggatgct cgaattgaaa taaatcaagt aattgcagaa tttgagcgga
    56401 ttgcggacca agcaaaagaa ccagatattc gtcaatactt gttagaaatg aaagcagagt
    56461 tggaagtaaa tggtattaca gatgagtaac ctaaaccaac acaactgact gttaggcgct
    56521 gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc
    56581 gttagccgac catgatgtct cacccccgta tctaacaggg gggtacaaaa acacaaaaaa
    56641 taagtaagca accattaaga atgcttgttg tatcaaagat agtaagtaat ttgtataggt
    56701 gttaactcat ttgaaaaagt taacaccttg aatttggagg tgagaacttg gcacaacgga
    56761 atttagatta tcggctatta aaagaccgac gcaacgaata cggcgtttca caaaataaat
    56821 tggctacggc ttgtggactt agtcggccgt acttaaatca gatagaaaat ggtggcgtaa
    56881 ccgcatctat aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt aatcccgatt
    56941 tgcctttgac gttactgttt gactatgtaa ggattcgctt tcccacaacg gatgcacgaa
    57001 aaattattca agaaattctc catttgaaat ttgagtatat gctccatgaa gattatgcct
    57061 tttactccta tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag
    57121 aagataaagg tgtgctttta gaattaaagg ggcgaggttg ccgacaattt gaaacttttt
    57181 tactcgctca aaagcgtagc tggtacgact ttttcgagga ttgtctaaag gccggtggcg
    57241 tgatgaaacg cttggacttg gcaatcaatg atcgagtggg gctattggat atacctgagc
    57301 taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttt aaaagttatc
    57361 gttccgggga acttttaaag gctgatgaaa aggacggcat gggcaatacc ttatatatcg
    57421 gcagtctcaa gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa caatatatca
    57481 agttagggat tccattagac caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa
    57541 atgaccgtgc ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt gagagcacca
    57601 cgttttccat tatcaatcgt tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa
    57661 ccaactggcc cttgaatgaa caatgggggc gttttattgg acggaatcgg aaagagattc
    57721 aattaacttc cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg ctaggccgac
    57781 aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcgg acaacctata
    57841 tccaggatat ggtacagaat gcacgcttat cagaccgaca taaaaagatt ttggagcaac
    57901 aaagtatggc aattgaaaat ttgattatat gaaaagagga atcgtcatgg gaattgtaaa
    57961 agatattttt ttggtatttg gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa
    58021 tgcaagtgca gcagcggatc gagcaatgga aaagaaagga aagtgacaag atgaatttcg
    58081 gacaaaattt
//
LOCUS       DACEPD010000007        56736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.007, whole genome shotgun sequence.
ACCESSION   DACEPD010000007 DACEPD010000000
VERSION     DACEPD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.007"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            101..571
                     /locus_tag="IW797_000391"
     CDS             101..571
                     /locus_tag="IW797_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ1223191.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            793..1896
                     /locus_tag="IW797_000392"
     CDS             793..1896
                     /locus_tag="IW797_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1223192.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            2117..3076
                     /locus_tag="IW797_000393"
     CDS             2117..3076
                     /locus_tag="IW797_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1223193.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            3246..3926
                     /locus_tag="IW797_000394"
     CDS             3246..3926
                     /locus_tag="IW797_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ1223194.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            4016..5050
                     /locus_tag="IW797_000395"
     CDS             4016..5050
                     /locus_tag="IW797_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ1223195.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            5064..5399
                     /locus_tag="IW797_000396"
     CDS             5064..5399
                     /locus_tag="IW797_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223196.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            5383..7863
                     /locus_tag="IW797_000397"
     CDS             5383..7863
                     /locus_tag="IW797_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223197.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            7919..9478
                     /locus_tag="IW797_000398"
     CDS             7919..9478
                     /locus_tag="IW797_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1223198.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            9475..10839
                     /locus_tag="IW797_000399"
     CDS             9475..10839
                     /locus_tag="IW797_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1223199.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            11010..12080
                     /locus_tag="IW797_000400"
     CDS             11010..12080
                     /locus_tag="IW797_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1223200.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            12095..12763
                     /locus_tag="IW797_000401"
     CDS             12095..12763
                     /locus_tag="IW797_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1223201.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            12783..13361
                     /locus_tag="IW797_000402"
     CDS             12783..13361
                     /locus_tag="IW797_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1223202.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            13762..14673
                     /locus_tag="IW797_000403"
     CDS             13762..14673
                     /locus_tag="IW797_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1223203.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSEKKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(15051..15968)
                     /locus_tag="IW797_000404"
     CDS             complement(15051..15968)
                     /locus_tag="IW797_000404"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ1223204.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            16170..16607
                     /gene="zapA"
                     /locus_tag="IW797_000405"
     CDS             16170..16607
                     /gene="zapA"
                     /locus_tag="IW797_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ1223205.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            16665..17213
                     /locus_tag="IW797_000406"
     CDS             16665..17213
                     /locus_tag="IW797_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ1223206.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            17328..19688
                     /locus_tag="IW797_000407"
     CDS             17328..19688
                     /locus_tag="IW797_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1223207.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            19775..20089
                     /gene="trxA"
                     /locus_tag="IW797_000408"
     CDS             19775..20089
                     /gene="trxA"
                     /locus_tag="IW797_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ1223208.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            20271..22049
                     /gene="uvrC"
                     /locus_tag="IW797_000409"
     CDS             20271..22049
                     /gene="uvrC"
                     /locus_tag="IW797_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ1223209.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            22194..23093
                     /locus_tag="IW797_000410"
     CDS             22194..23093
                     /locus_tag="IW797_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1223210.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            23086..24348
                     /locus_tag="IW797_000411"
     CDS             23086..24348
                     /locus_tag="IW797_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1223211.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            24509..26407
                     /locus_tag="IW797_000412"
     CDS             24509..26407
                     /locus_tag="IW797_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ1223212.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(26737..27456)
                     /locus_tag="IW797_000413"
     CDS             complement(26737..27456)
                     /locus_tag="IW797_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ1223213.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            27793..28125
                     /locus_tag="IW797_000414"
     CDS             27793..28125
                     /locus_tag="IW797_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223214.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            28112..30115
                     /locus_tag="IW797_000415"
     CDS             28112..30115
                     /locus_tag="IW797_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ1223215.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            30134..30604
                     /locus_tag="IW797_000416"
     CDS             30134..30604
                     /locus_tag="IW797_000416"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ1223216.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            30625..31209
                     /locus_tag="IW797_000417"
     CDS             30625..31209
                     /locus_tag="IW797_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ1223217.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            31224..32210
                     /locus_tag="IW797_000418"
     CDS             31224..32210
                     /locus_tag="IW797_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ1223218.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            32200..32511
                     /locus_tag="IW797_000419"
     CDS             32200..32511
                     /locus_tag="IW797_000419"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ1223219.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            32533..34314
                     /locus_tag="IW797_000420"
     CDS             32533..34314
                     /locus_tag="IW797_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ1223220.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            34307..35683
                     /locus_tag="IW797_000421"
     CDS             34307..35683
                     /locus_tag="IW797_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ1223221.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            35690..36325
                     /locus_tag="IW797_000422"
     CDS             35690..36325
                     /locus_tag="IW797_000422"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ1223222.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            36355..37710
                     /locus_tag="IW797_000423"
     CDS             36355..37710
                     /locus_tag="IW797_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ1223223.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(37902..39296)
                     /locus_tag="IW797_000424"
     CDS             complement(37902..39296)
                     /locus_tag="IW797_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1223224.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            39388..40359
                     /locus_tag="IW797_000425"
     CDS             39388..40359
                     /locus_tag="IW797_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ1223225.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            40356..41138
                     /locus_tag="IW797_000426"
     CDS             40356..41138
                     /locus_tag="IW797_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ1223226.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(41307..41678)
                     /locus_tag="IW797_000427"
     CDS             complement(41307..41678)
                     /locus_tag="IW797_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1223227.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            41812..42255
                     /locus_tag="IW797_000428"
     CDS             41812..42255
                     /locus_tag="IW797_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1223228.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(42298..43107)
                     /gene="yidA"
                     /locus_tag="IW797_000429"
     CDS             complement(42298..43107)
                     /gene="yidA"
                     /locus_tag="IW797_000429"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ1223229.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(43126..44493)
                     /locus_tag="IW797_000430"
     CDS             complement(43126..44493)
                     /locus_tag="IW797_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1223230.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(44477..45307)
                     /locus_tag="IW797_000431"
     CDS             complement(44477..45307)
                     /locus_tag="IW797_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ1223231.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            45470..45907
                     /locus_tag="IW797_000432"
     CDS             45470..45907
                     /locus_tag="IW797_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ1223232.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            45983..46534
                     /gene="rpoE"
                     /locus_tag="IW797_000433"
     CDS             45983..46534
                     /gene="rpoE"
                     /locus_tag="IW797_000433"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ1223233.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEEDLPGNIEEDL
                     SIIDEDDDDEDYEEEEFSDSEKE"
     gene            46723..47229
                     /locus_tag="IW797_000434"
     CDS             46723..47229
                     /locus_tag="IW797_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ1223234.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            47246..48145
                     /locus_tag="IW797_000435"
     CDS             47246..48145
                     /locus_tag="IW797_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ1223235.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            48232..49116
                     /locus_tag="IW797_000436"
     CDS             48232..49116
                     /locus_tag="IW797_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1223236.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(49308..49379)
                     /locus_tag="IW797_000437"
     tRNA            complement(49308..49379)
                     /locus_tag="IW797_000437"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49344..49346),aa:Arg,seq:ccg)
     gene            49439..50773
                     /gene="rpoN"
                     /locus_tag="IW797_000438"
     CDS             49439..50773
                     /gene="rpoN"
                     /locus_tag="IW797_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ1223237.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            50976..52013
                     /locus_tag="IW797_000439"
     CDS             50976..52013
                     /locus_tag="IW797_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ1223238.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            52054..53055
                     /gene="gap"
                     /locus_tag="IW797_000440"
     CDS             52054..53055
                     /gene="gap"
                     /locus_tag="IW797_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1223239.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            53165..54355
                     /locus_tag="IW797_000441"
     CDS             53165..54355
                     /locus_tag="IW797_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ1223240.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            54459..55214
                     /locus_tag="IW797_000442"
     CDS             54459..55214
                     /locus_tag="IW797_000442"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ1223241.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            55330..56628
                     /gene="eno"
                     /locus_tag="IW797_000443"
     CDS             55330..56628
                     /gene="eno"
                     /locus_tag="IW797_000443"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ1223242.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        18874 a         9863 c        11994 g        16005 t
ORIGIN      
        1 agaatctctg aagaataaat cacgtgatcg tgacgataaa atacacttac gtcaggtgat
       61 aaaaaataca taagtagaaa actaagagga ggaacagaaa atgaaagaag ttcaattgat
      121 cagaaaaagc gaactatcag aaggtggttg caatgcatgc ggcgtagtag aagcaacaag
      181 ctatacatta aagcttggtg ccaacaaagc gataatctct gagttgacag taggcggtct
      241 tgtggacagt ctggcgcttg ctgaaggttt tattggagaa gatatttatg agatgttttc
      301 agaaatccgt cagttaaaaa aaggggaaaa ctgcatagag gttcatcatg agagcccgaa
      361 tgtgagattc aaacgtggtg ataatgagat gatcttcaat aatcatgtgt ccgatcacac
      421 cgagctatat ggaatcgtga atcaaatact aactgagctt tttggattgg gaccttatgc
      481 gttcaaggaa gaaaacggta atccaaaatt aaacgaagaa tggcaagaaa cgattgaaat
      541 acaaagaaac aacccgcact tatttcaata ggcatccaca gtatttcgta aaagggactt
      601 tgacacgact tttgtcaaaa tcccatcgtt ttttatctat ttgtgttcta aacatttctt
      661 tcagagtagt aacaactgga atgactgaaa tgtaaacaga ttatttgcct gtatcaatac
      721 ggaagagtaa tataaagtgt gtaaacgcta tcatattatt cattcaattt tttatagagg
      781 gaaggcattg aaatggaaaa aacgtatcaa gcaggtacaa atgaaggaat agtggatttt
      841 atcaatatgg aagatttaga aatagccgct tcacaagtga ttccagctgg tggttatggt
      901 tacatcagca gtggtgctgg cgacttattt acttatcaag aaaatgaacg ggcattcaat
      961 catcagctga ttattcctca tgtattaaga gatgtagaat tacccgatac aactacacat
     1021 tttgacaaag aaacgctgac tgcaccaatc atcatggcac cagttgctgc tcatggctta
     1081 gctcatgtga aggcggagaa agcttctgca aaaggtgtag cagattttgg gacgatctat
     1141 acagcaagtt cttacgcttc ttgtacatta gaagagataa gagaagcagg cggggagaaa
     1201 gcaccgcaat ggtttcaatt ttatatgagc aaagatgatg ggatcaattt ggatattctt
     1261 gaagtggcca agcgtaatgg agcaaaagca atcgtattga cagcggatgc aacagttggc
     1321 ggaaaccgtg aaacagaccg cagaaatggg tttaccttcc cactgccaat gccgatcgtt
     1381 caggcttatc aatcaggagt aggccaaacg atggatgctg tgtacaaatc ttccaaacag
     1441 aagcttagtc caaaagatgt agaatttatt gctgctcatt ctgatttgcc agtctatgtg
     1501 aaaggtgtcc agtcagaaga agatgtctat cgttcattag aatctggtgc tggcggaatc
     1561 tgggtatcta atcatggtgg ccgtcaacta gatggtggtc cagcagcgtt tgattcgttg
     1621 caatatgtgg cagaagcagt agataaacgt gtaccaatcg tgtttgacag cggtgtacga
     1681 cgcggacagc atgttttcaa agcaatcgca tcaggtgcag atcttgttgc tattggcaga
     1741 cccgttattt atggattgtc tttaggagga agtacaggag tccatcaagt gtttgatttc
     1801 tttaaaacag aattggaaat ggtcatgcaa ttagctggaa cacaaacagt agaagatatc
     1861 aaaaaaataa aattgcgtga gaatcgtttt atttaagaaa caaatgacga aaaaaatagg
     1921 agtgcggcag atgatgccgt actcctattt ttatagttca aggatgtcac taatagaata
     1981 atttgaaacg aaaaaataaa attgaaatat tttttcatac ttcgccgatt gtaaaattaa
     2041 gctagacaac taaaaaaagt catttgtgct acactcaaaa tagttccgac gaaagaatta
     2101 taaggagtga gtacaaatga cctataacca tcttacacca actgaactcg taatgataga
     2161 agcttatttc aatcaatcac aacctgtttc aaaagtagcc aatctgttac aacgttctcg
     2221 acaaactatt tataaggtct atcgttttct taaatctggt ggaagcgcaa tgagctacta
     2281 taaacaatat aagaaaaaca aacgtaactg cggaagacat ccgattgttt tacctgatga
     2341 gcaacaggaa tacattcaaa aaaaggttgt tcaagggtgg gctcctgatg tccttattgg
     2401 tcgagcagag tttcctattg cttgttcggt acgcacactg tatcgaatgt ttaaagataa
     2461 aacctttgac actcacgatt taccaatgaa gggaaaaaga aaaccaaacg gtcataaaga
     2521 aaaaagagga aaacaagggt ttcgccggtc aatccgagac agaaagacag actataccca
     2581 gtttgatcat gaatttggtc acctggaagg tgacactatc gtgggtggaa aacataaaag
     2641 cgcggtcatc acattagttg aacggctatc taaagtcatc attaccttga aacccgaagg
     2701 cagacaggct aaagacattg aaaataggct gaatcaatgg tttcgttgtg tcccaaaaaa
     2761 cttatttaaa tccatcacat ttgattgtgg aaaagaattc tcaaattgga aaaagattag
     2821 taataccaac gatatttcaa tttattttgc ggatccagga acaccttctc aacgaggatt
     2881 gaatgaacat tcaaatggct tattacgtaa agatggtttg tataaagcta tggattttaa
     2941 tggtgtatct gaaaccttta tccaatcggt ggcatcgaaa cgaaatcata ttcctagaaa
     3001 atcattacac tatcggacac caatggaagt atttttgagt tacgtcacaa aagatgattt
     3061 gtctaactta atttgacaaa cgaaacttaa taaaaaagga agaatattca tgtaaacgat
     3121 tttattgttt ttttctcttt tatacaaaaa gggtcataat ttcaagtaag cggatgaatt
     3181 catgaggatt ttttgcatac tgtctctact atacttaggt cactaagaaa aaggagtgat
     3241 ttattatgaa aaagaacatg attgggacat tattactttc aacacttctt ctaggcggct
     3301 tggcaacacc agcttttgct gagggtcaag cgacttcaaa aggagatatt acctttactg
     3361 aaccaacaaa taccgtggag cctcttaacc ctactgatcc ttcaaaacca gtagagccag
     3421 cagatccgga aaatccagca actggccaga caggtccttt aacactggat gtagttcccg
     3481 aattgccatt tggtacacac gagattgaaa gcggaacaaa aacgtatcaa gtagacgctt
     3541 caaaaaacga tacaccttat ctccaagtat cagatcgtcg aggtgtaggt gcagatggcc
     3601 aagcacaagg gtggaacgtg acagtttcgg tttcagattt tgtaaacggt tcacaagttt
     3661 tgcaaggcgc tgaattagat tttggaacat ccactgttaa aagtacgtca gataacgaat
     3721 ccactggacc aactagtcaa actgtaactg ggttgagtaa agcaagtgca gcgacaccaa
     3781 tatttacagc agcaaaagat caaggtctgg gaacatggct ttctgtttat gatccagcaa
     3841 atatcacact gaaagtgccg aaagcagcag ctggaacatt cacggctgat ttgacttgga
     3901 acctcgtggc tggcccagta gcatagagta tgaaataaat tcaacaaact gcggtgaaaa
     3961 tactataggt gaaggatttt ttaccgcagt tgttgttgaa aggatgtggt ggacaatgaa
     4021 tcaaagtaaa cgactgtttg tttcgttttt ttctatttta agtctatttt tcatttttcc
     4081 gtcaatcagc caagcaaagg attcagcagg agactttgga atcaaaccgg tatttccaga
     4141 gaaccaaata gataaagcaa tcggttattt tgatttatta gtggcaccgg aacaaaatca
     4201 gacactagaa gtaattatca gcaattcttc agatgaagaa cgaacatttg aggtctctgt
     4261 caatccggcg gtaacgagtg atggtggaac gatcgattat agtcaaaaaa acccaacgtt
     4321 agatgagaca ttgccgtttg atgtccgtga cgttttgctc attgcgaaaa aggaaatcaa
     4381 tgtatcagca catgcagaaa caactgtacc tatagaagtg aaaatcccag caaaaagctt
     4441 taagggacgt gtattagcgg gtattcatgt ttcacctaaa gaagaagctg aaacagaaaa
     4501 tgctacagaa ggagcacaaa tcaaaaatcg tattgcttat aatctcgcag tcgttttaca
     4561 agaaagtcaa gagacgatag aaccggatct gaaactttta tcaggagatt tggacgaggt
     4621 caacgcaaag ccaactgttc agcttcgttt ccaaaatcct cagcctagga ttatcagcaa
     4681 tttgatattt acgagcaaga tcttttatga aaatcagctt tatattgaaa atacgtctaa
     4741 tgcattttta gttgcaccaa attcgaattt tcacttgaat cttgatttag ctggtgataa
     4801 agcaaaagcc ggcgattacc gcgcagaaat catagcaaaa tcaggcgaca gtaatgaatg
     4861 gcgatttaca caaaacttta cgatcaaaaa agaaaaagca caaaaagtaa atgaaaattc
     4921 cgtttttgca gtacaagaac aatctttccc atggctttat gcaggattgg ttgctgcagt
     4981 tctagtgtta tggttgattt tgctgttttt gtgggtccac aagaaaaaga aaaaaggaga
     5041 aacaacatga tgaggtgatt tcgttgaaaa catcgatctt attgggaggg attattgcaa
     5101 gtttgctgtt cttcagctgg caagagccag tccaagcaga taccaaaagt tcaggtacga
     5161 ttacatttta tgatctaagt ccaattccgc atccaagtgg ttcagtcaat ggcgcatcaa
     5221 aacagcagac acagctaccg atttataaag aatctaatca acatggcgta ttcccggaga
     5281 cgaatgaaca aaagatccgt tttctttatt tgatcattgg tatagaatta tggcttcttg
     5341 cgtggttgtt gttatggaga agaaaggaaa aggaggatga acatgttgct acaaaataaa
     5401 ttttcatttt ttcgctcctt cttttttact tcgctttttg ttttaagtat atttatcttt
     5461 tcaccttcgg cagaagctgt tggagcaacg cctcctgcca tttcttatga tccatacacg
     5521 tatgaaaata tcgtacctcc aaatggtgga cgtagtgcag tcaatggcgg gaagcttgtc
     5581 caactaactg gggctaacca attgcctaaa ttgactgcta cttatttgaa cgatggttca
     5641 aatgatcctt ggaaaggtgg cacggtccgt tttcattggg aaagccatgt gaaactaaca
     5701 aatgtacttg atttggtgaa tatcactgca caaggatttg catttgtggc gatcatcaat
     5761 ttgccggaac atgtatcaag cagtgatgta ttaaaagcaa ttgactggaa caacgcctac
     5821 ttgtatattg ccggtgcccc catcaaggtt actagcggaa gtttattatc agcaggaagc
     5881 cataccttgc gttttgggat ggggtcgtgg gatacaaata ctctattgac agatattatt
     5941 gctattatta cgcgaggcga attcaacgtg gacgatatcc cgcttcaatt tgatgttaca
     6001 gctaatgtcg cgaagatgac agaaaacgga gataaatatg atacgagtcc aaacaaaatt
     6061 ttgaccaagg gtaaattaca accttctatg tctaaaaagg ccgttttttc tgttgatttt
     6121 tatgattcga ataatattat ccaagatcgt atgttcaatt cgattttagc tccagctgca
     6181 ggtagatttt atcctaaatt aactggcgga aacttgaatc caggagcata tgcattttct
     6241 gcaagtgcag agataaatac gtggaatagt tacatctcac caagtgattc aacaggaaaa
     6301 tataatacat ttgctgatac cacagaatac gtggatggaa ataaccagaa ccttccaggc
     6361 tctaccaata gtcggacatt gacgatgggg ctgactgaag gacgttttga tcagttgcca
     6421 tttagtcgct ttgatcgcgt agtcaactat tttacaggaa aaaacgttac acaaggagca
     6481 atactgactc atactccagc aagcacaagt ggattgaatc agacagccaa tatcgtgtac
     6541 agtgggaaag atgcgcaagg cggattgctt tctcctgttg ctttaaaggt attggagcag
     6601 tacaagctag acggaacggt tttaccaaca aacttaacag ccgatagtca gtggggaaag
     6661 ctcctttctt ctgcatcgta ccaagtcacg gccaattggt cagcaactga actaaaaaag
     6721 ggttcgatcc attacaggct ttattcgaaa ggaacccaaa aattagcgac aggatggaca
     6781 gatcaacttt tccaaacgat ccaatcaaat aacgggggtg taaatacagc tacttctaca
     6841 ctacctgcat tggcttcagg gcaatattat tttgattatc gcttggtgga cgatgtactt
     6901 actaacatgt atccgtcttt tgcgtacaaa tggcagtctg aacaagctgg aatcaccact
     6961 cttcctcaaa taacagtggc taattttcca tctgtgagtg cagatagtca gctaaaaaac
     7021 cttagcagac aacctgagac aggagatccc ctgtctgctc tatcaggaga tacgattcaa
     7081 gaaaatgtga cattcacgtt aacaaaatct ggggatgcga taaccgacaa aaaaatcacg
     7141 atctcacttc ctgaaaatac tacttatatc aatggttcct taaaattaaa tggtacaact
     7201 atcgcagata ccggcattca acaaggagtt tctgtcccag cagatctatt gagcaaaata
     7261 ggagatacca ttcatcttac ttacaactat caattaaata ctgtagatac ttctttacaa
     7321 tccgtcagca tactgacaaa ggcagctgta ctttccagca atattacatt agcagatggt
     7381 gctaagcttc ctaacccagt tgtccaaaca tctgctaaaa caattcttgt ccccaaacaa
     7441 gaattaacat tagttaatgt accagatgat tttacatttg gtaacgatct ccccaaaccg
     7501 ttgaagacaa gttattatga atcaaaaggc gatttttctt ttgatgtgcg ggatactcgg
     7561 cttccaagca cgtctccttg gcaactgaca ggtacattga cttctttatt taagaatgat
     7621 caaggtcaag aattaagcgg aacaaaatta tacttcaatc attctggatc aaagcaattg
     7681 attcagcaag gtcaaaacac gttgatttat gagagtgatg gaacagctaa aggagaggtt
     7741 ctcgttgact ttcccgatac cgatggttta ctcttagagg taaatagcag tacgaatgct
     7801 caacctggcg caacgtatca aggcatggtc acatgggaac tgacagctgg gccaaccagc
     7861 taagtgatta gaatttgtat ggatctgtga taactgtcac agatcctctt tttcaaggat
     7921 gagaaggaaa cttctgctgt taaaatcaga aatgcttgaa ggagtggact tggaactttt
     7981 cagtttgctt gaaaaaaatg aacaacggca aatcaaactt ttccaacttt tattagagga
     8041 aaaaggagag gtttccgtta ataacgtact tacccgctta tcagttgatc gggctacctt
     8101 gaaagaagat ctgttttatt tacaagctaa cttggaaaat atcaaacaat tggaaattgt
     8161 tatccaagaa gggaatattt tattaatcag acatggaaat atagcagcag tcgatgttta
     8221 ttatcattgt tacttgcgct attctacaaa atatcagata cttctttatc tgttggaaaa
     8281 tggctcattt gatcggcaga aacttgtcat ggaacttaat atcagttcgg ctactttgtc
     8341 tcgcagagtg aaggaattaa atgattcttt aaatgagttt catctgaaaa taaaaagcgg
     8401 gcggctgacc ggtccagaaa gtcaaattcg ttatttttat ttccaattat tttggtttgg
     8461 acgtccctat cttgaaaaca aaaaagaata cagtgattta tcaggaaaca cttttttaga
     8521 tctgctgcaa aaagagttta gttttgctat tacagaagaa ggacgattga aatttgaaat
     8581 atggctttcc attatgaaga agcgaatgcg ctgtacgaaa aaatccaaac cagattatag
     8641 caatcttcct gttggattgt ttcaaacgac acaattttta ttggacctcc atacattttt
     8701 gacccgttac ttctttcaat atgcctttcc ttggaatcaa aatgaaacgt tggtttttta
     8761 tcttttcatg atcagtaact ttacattaga tacacataac tcgcatgtcc aggagttttt
     8821 gataagcgaa aaatacaaag acgacgtagt agctcaattg aatcgaacgt ttaaacaatc
     8881 gatagaagaa tatttctctt tttatgtttt tccagaagag atttcgcaaa agattgcgtt
     8941 gacagtgatg caaaatcacg ctcattttgc ctattttaga ggatggatga atatttttgg
     9001 ccaagaagga ttaaaacagc ggttaactga agtagaagat aaacaattcc tggttttatg
     9061 tgaaagattg gtcagacaaa catttgctat tgttgatttt actgaagtag acgaggaaaa
     9121 tgcccgcttt gaattatttg ctcgctatgc atctatttta cggacgctga tcaagcagtt
     9181 agaattacgt ctgcaaattg cttatgatct tccttatgaa aggctaatgg cggatatcgt
     9241 gaaagaaatc atccaggaac aattgctgaa tacgatcaaa tatgatttag tcgaatacga
     9301 gaaagaaaag cagtatgata tcattttgac tagccaattg aaagagtacc cgtcacagaa
     9361 gtccgctaaa gtttttgtat tcactagcaa cgaatataat tatgatttcc cctatttgaa
     9421 ccaattttta aaagaatgtt atttagaaaa attgaattta aggatgaata aaacatgaaa
     9481 atcgtgatca ttggtgcttc ccatgaaggc atcacagctg ccctaaatct aagaaaactt
     9541 caaccgaaag cagaagtttt actgatcgat gaagatcata aagatggact aggctatgtt
     9601 tcaaatggaa tcaaccttta tttaaaagga aagatacgta gtttagctga agttgcccat
     9661 aacatgcgga ctcttcaaga aagtggggca aaactgatca ccgaatggag agtaactgaa
     9721 cttgatccag acaaacatca gctcatcctc gcatccaaag aaggaaaaaa cgagaccata
     9781 acctatgata aattaatcgt agcgacaggt tcctctcctg tcaccttgta taaacaaatt
     9841 gaagctgaaa atgtctacac ctataaaaat ctagtgcaat ctaagcaagt acttgccgct
     9901 ttaaaagaag caaaagaagt agtgatcttc ggtgctggtt acattggatt ggaattagca
     9961 gatgctttga gaaataaagg atacattatt catctagttg attatatgcc gaatgtttta
    10021 tcaagatatt tcgataagga tatgatcaac agttttcaaa atcagctgca aactaagcaa
    10081 ataaattttt atccaaatga attcttgatc gattggaaaa aatcagaaga gaaagtagta
    10141 tccgtacaac ttctgtctca aacaataaag gcagatatgg tcattttttc tgcccaaact
    10201 cgtcctaata cgacattgtt aaaagaaaaa gtggctttgt atgaggatga gactgtgata
    10261 gtcaatgaat atctgcagac aagtgatccg gacatctatg cgattggaga tatcgtccca
    10321 gtcagttttg acaaaaataa acggcatctc tttttacctt tagttactcg agctgtacat
    10381 atggcaagag cagtagcgct aactttgagt ggtcagccaa ccgcttacga cctcagacaa
    10441 aagataacag cagcagtcat tatagactac tttttaggaa ctgtagggct gacagaagat
    10501 gaagcgccat tcttggaaca aaataccggt tcgtgttcag gagaatttga tctatttcca
    10561 caatacgacg aagaaaacaa aacagtaaat gcaaagctga tctaccatcc ggatacatta
    10621 gaaataattg gcggacagct gataagccaa gaattcttat tatcagattt gaacctgctt
    10681 gcggatatcg tgaaacacaa aactacgatt ccacagctag cagttgagga ttttggattt
    10741 ctgtctgaat acacctctcg ttttcactac ctcaatgagt tagcttttaa agtactagct
    10801 aagagagcaa atagcaatag agtagataaa agactgtgag aaatatcacg gtcttttatt
    10861 gtgtggggat ataataaata ttaatcaatc gagaataaaa aattcatata atgaaatagc
    10921 ggaagaattt tttgagcata atattcgcca aaaatttatc aagtgataaa attgttgtat
    10981 aaaatgatta tttcaggagg aggagttaaa tgtttttagc attaaatgaa attatgcatt
    11041 caaagttacg ttatgcgtta gttgcagggg tgatgttttt gatcgcctac ctggtatttt
    11101 ttttgactgg gttagcttat ggcttagctc aagataatcg aacagcagtc gacaagtggg
    11161 aggcagattc gattgtgctt tcgaaagatg caaattcaaa tctagggatg tccatgatta
    11221 ctaagaagat agcagaagaa gtcgaaggtg ggaaagtggc gtatcttgcc caaacacctg
    11281 gagtcgtgac tagtaaagac agtacagaag aaggaaaaat caatgttagc ttcttcggta
    11341 tcgataaaaa tcaattcatc atgcccaatc ttgttgaagg aaaagctttt gataacgatg
    11401 atgaggcagt aggcgatatt agtttgaaag aagaatatgg tctagccata ggagatacag
    11461 ttaagttatc tggtagtgac aagacgttta aactaactgg ctttacggat catgccaaat
    11521 tcaatgtctc accagttctc tatacaacca tcaatgctta ccaagaaatc cgcttcgaaa
    11581 aagaagatac aagtgaaaat gcacggatta acgcaatagt tgtcagaggt aaaatcagta
    11641 atttacctga agatttagaa cagatcaaaa tttctaaatt catcaatgaa ttacctggat
    11701 ataatgcgca agtattgact tttggattta tgatcggatt tttgatcgtt attgcggcta
    11761 ttgtgatcgg gattttcatt tatgtactga cgatgcaaaa gattaatatc tttggtgtaa
    11821 tgaaagcaca agggatcaca ggaggattta ttgcgcgttc tgtagtggca caaacattca
    11881 ttttatcatt tgttggtatt cttcttggtc tattaggaac agtgggaaca tcccttgttc
    11941 tacctgatgc ggttccattc caaagcaatt ggctgttttt tggcgtcatt agcttgttga
    12001 tgttagtagt agctgtgtta ggcgcactat tttctgttcg gacaatcgta aaaatcgatc
    12061 cgttgaaggc aattggataa gaaggaggaa gaacatgaaa gcaatagaat ttaaagaagt
    12121 agaaaaaagt ttccaagatg gcgaccaagt agttaaagca ttgaaagaaa caaccatgag
    12181 tatcgataaa ggggaatttg ttgcaatcat cggtccttct ggttcaggta aaagtacttt
    12241 tttgactttg gcaggcggat tgcagacgcc tagcagtggc tctgtattta tcaatgatca
    12301 agaattcagt aatcaaaaag aaagcgaacg cgttaaacta cgttttaaag aaattggctt
    12361 tattttgcaa gcttccaatc tggttccttt tctgacagta aaaaagcagt tattattagt
    12421 agataagatt aaacatgaac atcgtcaaaa agaagcaaac gaattatttg agcagcttgg
    12481 cgtggataag ttgatcaaca agtatccaga agaactatct ggtggagaac ggcaaagggt
    12541 cgcaatcgct cgtgcattgt acaatgatcc gtccattatt ctagcagatg aaccaactgc
    12601 tagtttggat tcagaaaagg cacgagaagt agtcgatatc ttggcaaaag aatcaaaaga
    12661 caaaaataaa gcaacgatca tggttactca cgatacacgg ctgattgaca aatgcgatag
    12721 aatatttatg atggaagatg gtgtattgaa agaaaaagaa taaccaaaaa gcaggtggaa
    12781 aaatggtcca tcgatacaac aaagatacac agcaaaaaat tttgaaagaa gcgactgact
    12841 tgtttatgtc caagggatat ctcggcactt caacaagaga aatcgcacaa aaagttggga
    12901 tcacccagcc caatctctat cattatttcg gtgataaaga aaaactatat acggcagttt
    12961 tagaaaacca cttaaaagat gttggaaaag ctttgagaga aatcgttcaa acaagcgaaa
    13021 cgggatttca agtaaccttg acaaaaatgg cgcagttttt gattgagaca catctggtcg
    13081 atttgtttct gatgcttcac gatcttgaaa gcaatctttc aaaggaaaca agagatcatc
    13141 ttttctttct atggaagaaa aattatcgtg aaccttttga agaaatcttt tcagcaaacc
    13201 aatctgtgct aagaaatggg atttcaaaag aaatcgcagc aaggcatttt ttcttagtgt
    13261 tagcacctta catcactcgg tcgaaaagac ctgtggaaga accgctaaat gtggcgcaat
    13321 tgatcgattt atatttgaat ggtgtattag gagaagcgta aagctgttat atagggagaa
    13381 aatagttagg tgaagaagca agatatgacg aagatcgtta tatcttgctt ctttatatag
    13441 gtagtttttc ataaatacaa atttgttatt ctctcatgat tatcagggta tatttttatt
    13501 tattatgtcg gtgttccagg aagtgtttta atttaataac gaagaaatta attgtttgta
    13561 gaatgaagga aactatttat tgggtagatg aatcaccacg aatagtcaaa aggagatttc
    13621 aggatatgcc aaaaatgaaa aaaatcattt tagcagtaat gttattttct acggttatat
    13681 cttttacagc cccttttttg cttacttaag ctgattttaa gttttggccc ttagtctgga
    13741 tctacgaggt gagagtcaag aatgaaaaaa ataattggag cttttgtatt tgcaatcatt
    13801 atcattatta tctgttttca aatagatgtt tttggtcaaa aaaagatagt tggacaaacg
    13861 gtcagcagta cgaacagtac ggttacaaag acaaaggaat catttgatga tacagaacaa
    13921 gagacattaa tcaaaatcgg aaaaaatgac cgtacactat atacagatga ctcgctaact
    13981 accccacttg caactataaa cggcggagag ctggcggagt ttctttctga aaaaaaaaat
    14041 gcttatcaga tcaagacaaa tgatggctat actggatatt tagccctcga agatggaacc
    14101 aaagtcacaa agaatataca aacaaaaccg gaagagctat ctgatgcagt gattgtatta
    14161 gatcctgggc atggcggaaa tgatactggg gcattaagca atgatgaaca aacagaagaa
    14221 aaagatataa cattaagtac agccataaaa gtaaaaaagg cgctagagga tgctggagca
    14281 acggtttatt tgactcatac tacagatgaa ttagtacagc ttggagatat atgcgactac
    14341 agtgaagaaa aaaaggcaga tgtctttatc agtttacacg cagactctac ggaatatgcg
    14401 aatgaagcaa ctggaatcac gacatattat tactatggac aagaggaaac attagcacag
    14461 acaattgctg actccttcac tgatctgcct ctggattcaa gaggaatctc cactgggaac
    14521 tatcaagttc ttcgagaaaa tcttcaacct tctattttaa tcgagatggg ttatatgaat
    14581 aatgatagtg atttaaagga attagtaaca gacagttatc agcagcagat tgctgcaagt
    14641 ttgacacaag cattgaccac ttattttcag taataaataa gcccagccaa acaaaaatag
    14701 ctcctcatat ttttcgtttg gccgggctta ttatcgcaga tgcaagcttt taaacaccgt
    14761 ttaaggaaga ggtcgtgagc ctgccgttca actctgagtc acaaggagca atttcaaaaa
    14821 acagcttctc atgtttgatg actattgtga cttgtgaccg aggagttggc atgagaacac
    14881 cgtttaagaa aaaggttgtg aaatcaacgt tttgttctga gtattagaga aacaaacgga
    14941 aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgaggatcag atgattgaac
    15001 accgtttatt cggaacaagg gactgtgaca tgacttttgt cacagttcca ttattttttt
    15061 agtagcttca aagctttttc tgtattggca aaatgaagtt tggcatattt ttcaagcata
    15121 ggcatgccgc ctttctccaa tagttttgcc gctacttggt cagatttcgt tccagctcct
    15181 gacggaaggg tgattccagc ttcagctgat gctttgtcta actcttccaa aaagctggca
    15241 cgactgatga tagaagcagc agcaacagct aaatgatatt gttctccttt agtaacgaag
    15301 tacagttttt cttgtaattg atttttttct aaacgcgcat attttttaaa atttgcttcc
    15361 ggcgtaaact gatcgatcaa aatcgcttct ggtttttctg gctcgatttt ttttagcagc
    15421 aaatggatcg cttgattgtg caaagcgact ttcatccgaa cggcattgta ttttggttgg
    15481 atctcattgt atttttctgg agataagaca agcagtttat aggggatggt tttttttaag
    15541 atgtcagcaa gctggatgat ttggctatct tttaactctt ttgaatctct aacaccaagt
    15601 ttacgtaatt ctgcaagctg atcacgtttc acataagcag cacatactgt tagcggaccg
    15661 aaatagctgc catttccgac ctcatcacta cctaaaacgg ataattgact gatatttttt
    15721 ggcaaagaag aggctgtttt tgctggcttg gcttgtttat cctgtgcctc cccccattta
    15781 tcagcttcta ttgctgcatt cgtaccttga aacatcactt ttccggatgt ataagcggta
    15841 atggtcgtcc cgtctttttt tgcagcaaag ctagtataag gaatctgttt atttaacaaa
    15901 taagagcgat agtatgcttt catttgacta agttggtcgt ttgttgcttt gataatgacg
    15961 ttgctcataa aattctcctc gtttttatac ataaaactat accgcaactc acgacttttc
    16021 gttttctaag agattgcgag acatagcatt tttttaaagt ttatcatata atagttaagt
    16081 atgtgaaaaa tatttgtgga gaacggtgaa agaaccaact tcatggtaca ctaaataaaa
    16141 ctttcatcaa ataggaggaa atgattcgta tggcgcacga aaaaacacgc tacaaagcgg
    16201 tgattgccaa ccaaacatat actattatcg gacgggaaac caagcatcat atggatatcg
    16261 taacgaagct gatcaatgaa cagttagcag aattgaaaca attatcacca caaatggata
    16321 atgaacaagc agcaatcttg atggctgtca atgcgctttc ggatcaattg aagaaacaag
    16381 aaagaatatt ggagctggaa gaagaaacag cagaattaaa gaaaaaaatg atcaaattta
    16441 cagagcttga aaatcgtgtg aaacgcattg aagcaatcga gaacgaagct cgggaagtat
    16501 tgaaagaaaa tggtcaagca gatcatacca ttaaaaatca tgtggaagca caacagattt
    16561 taaacgagaa acgaaaagga caaattaaac aaaaatcttc taattaagat ccttttatca
    16621 aaataagaaa aatttaaaaa agttagaaga gaaaggaacg tagcatgctg agtttgctga
    16681 ttttattttt attgctaatt gcgttttttt ctggagcacg tcgcgggttt gctttgcaaa
    16741 tcatattcgc gattggatat gtattgtcat ttattgctgc ccagcatttt tacaaaccat
    16801 tagcaagtca tttggaatta tacattccat acccagctgt caccccaaca tcaaagttag
    16861 cattttttga tcaggtattt gcctttcacc tagatgaagc tttttatgca ggaactgctt
    16921 ttctgattat tttgctaatt ggctggctgc ttacccgatt tgtgggggtt tttgtccatg
    16981 gattgacgta tgtgccgatc ttgagacagg tagactggat tgcaggaggc atcctgtcac
    17041 taatcatggc ttacgttact atatttttga ttttgcagtt acttgccttt gttccattag
    17101 atgtggtcca aaatcaattc ggcgagcata gtttcgctcg ttttatcgtt gagagaacgc
    17161 catttcttac caacaagatg tatgagcttt gggtaacgaa tattttgaat taaggaaaat
    17221 agtgctggaa aatatttcgt tttttgtaca gagtagttta tttaagaagg ctgcgtagcg
    17281 tatgtccggc acagctcttt tttcatagtg aaagataagg tgataaaatg aataaccgaa
    17341 tccttgaaac attggaattt gaaaaagtca aacagatggt cagacaattt gtagtgactg
    17401 cacaaggaaa agaagaactg gcagaattgg taccagtgag tgagaaacaa acgatcacaa
    17461 actggcttca agaaactgag gatggattga aagtgcaacg tctgcgaggc gggatcccta
    17521 tacctaaaat tgaaaatatc cgtccccata tgaaacggat tgagatcgga gcagacttga
    17581 atggagtgga gcttgcacaa gtttccagag tattgtctac gacaagcgaa ttgaatcgtt
    17641 tcattgatga tctttctgac agtgaaatcg aatttgcacg tttgtatatg tgggcagatc
    17701 agttagtcac gatccctgtt ttgagccgcc gtttaaagga agcaatcgat gaagacggac
    17761 gcgtgactga tgacgcttct cctgagttaa agagcataag acagaacatt cggcgaagtg
    17821 aacaagctgt tcgtgaacag ttagatggga tcgtccgtgg gaaaaatgca aagtatttaa
    17881 gtgatgcgat catcaccatg cgaaatgacc gctacgttat cccagtcaaa caagaatacc
    17941 gcggtgtatt tggcggtgta gtccacgatc aaagtgcttc aggtcagact ttatttattg
    18001 aaccaaaaca agtggtggat ttgaataatc gtttgcgtca ataccaaatc gctgaacgaa
    18061 atgagattca acgtatttta agtgaacttt ctgctgaact tgttccccat cgtcaagaaa
    18121 tcatccacaa tacttatgtg attggtaaaa tggatctgat gaatgcaaaa gcacgatttg
    18181 gtaaagaagt aaaagccatc gtgccgggaa tcagtgaaga caatcatgtc gtcttgaaac
    18241 aagcaagaca tccactgatc gatcaggaaa aagtagtttc taatgacatc acaatcggaa
    18301 aagactatca agccatcgtc attacaggac ctaacacagg tgggaaaacg atcacgttga
    18361 agactttagg tctgttacaa ctgatggggc aagcaggatt accgattcct gcaggtgaag
    18421 aaagtcaaat cggtattttt gaggaagtat ttgcggatat aggagacgaa cagtcgattg
    18481 agcaaagtct atcgaccttt tcttctcata tgacgaacac tgtagatatt ttatcaaaag
    18541 tcaatgaaaa aagtctggta ttatttgacg agctcggtgc aggaacagat ccgcaagaag
    18601 gtgctgctct agcaatcgca atcttagatg atcttggtaa aaagtctgct tatgtgatgg
    18661 caacaacgca ttatcctgaa ttgaaagttt atggatataa tcgggcaaat acgattaatg
    18721 ccagcatgga gtttgatgtg gatacattaa gtccaacgta tcgtttgctg attggcgttc
    18781 ctggacgaag caatgcgttt gaaatctcta gccgattagg tcttgataca gaagttattg
    18841 atgaagcgaa gcagttgatg aatgatgaga gccaagattt aaacgaaatg atcacagatt
    18901 tagagaatcg ccggaaaatg gcagagaccg aatatctgga aatgcgtcat tttgtttctg
    18961 aggcacaaga acttcacgat gatttgaaag aagcttacag ctatttcttt gaagaacgtg
    19021 aaaaagaaat ggaaaaagca aagaaaaaag caaatgaagt cgtgtcagag gcggaagaaa
    19081 aagcagaaaa aatcattgct gatattcgta aaatgcaaca gcagattggg caaggaaatg
    19141 tgaaagagca tcagttgatc gatgcaaaga cccaattagc aaatctacat caagaagaaa
    19201 ctttgaagaa aaataaagta ttgaagaagg cgaaagaaca aaaaacgttg aaaccgggag
    19261 acgaagtgtt ggttacgact tacggccaaa gagggacgct tttacgtaaa aacgggaatc
    19321 agtggcaagt agaaatcggt atattaaaaa tgaatgtgtc cgaagacgag ctgacgcctg
    19381 ttgctcctca aaaagagccg acacaacgag ttatccatgc tgtacgatct gaatctagta
    19441 gtcacgtgcc gaatcaattg gaccttcgag gaaaacgata cgaagaagcg ctaagtgagg
    19501 tagatcaata tttggattct gccattctag ctggttatcc tcaagtgact attgtccacg
    19561 gaaaaggaac aggcgcactt cgcaagggaa ttactgatta tcttaaaaac catcggagcg
    19621 tcaaaagctt tgagtttgca ccggcaaacc aaggcggaaa tggtgcgaca attgttaaat
    19681 ttaagtaaag cttactaaag gtaagcagat ggttcgctat ccaagtatag aaatgctata
    19741 ctagtcatag attttaaaaa tggaggtctt tgatatgtca caagtaatta cagataagac
    19801 atttaatgaa gaaacagata aaggacttgt attgattgat ttttgggcaa cttggtgtgg
    19861 tccatgtcgt atgcaagcac cgattttgga tcagttggaa caagaatatg atgaagaaga
    19921 attcagaatt gctaaaatgg atgtggatga aaatccagaa actcctcaac aattcggtat
    19981 catgagcatt ccaacattaa tgttgaaaaa agatggtcaa gtagtagaaa aagctgttgg
    20041 tgttcattca aaagaacaac tacgtcaaat gatcgatcaa tatttataaa aacttacagc
    20101 aaaaataagc tgagggtggg actgaatcca gttccgtcct cggttttttt gcggttgagt
    20161 taaggttatt caaattaata ttttttgagt ataattacgt tgtcaggctg gattgttttt
    20221 ttgacagaca tgctataata actttaattg aatatgaagg ggagtaccag atgaacgaac
    20281 gaatcaaaaa caaattagct ttacttcctg atcagcccgg ctgttacttg atgaaagata
    20341 aaaatggaac gatcatttat gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat
    20401 attttactgg tagccataat acgaaaacgg aacggcttgt tagtgaaatc gaagactttg
    20461 aatacatcgt tacagaatcg aatatcgaag cattgctctt agagatcaat ctgataaaga
    20521 aaaatgatcc taaatacaac attatgttaa aggatgataa aacctatcct ttccttaaga
    20581 taacgaatga aaaatatccc cgtttggtga ttactcgtaa ggtactaaaa gacaaagcac
    20641 tgtattttgg accatatccc gatgtcggtg cggcgaatga gacaaagaag atcctcgatc
    20701 gattatttcc tctgagaaaa tgcaaaccta gtcagacgaa ggagccttgt ctttactatc
    20761 atttgggaca atgcctgtgt ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa
    20821 tcgttgatga agtgaaaaac tttttaaacg gaggacacga aaaaatcgag tcagagatcc
    20881 atcagaaaat ggaaaaagct gctgaaaaca tggagtttga aaaagctgca gaataccgag
    20941 accagatccg tgccattgag acaatcatga ctcgccaaaa aatgacaaat actgatttac
    21001 tggatcgaga tgtatttggt tatgcgattg ataaaggatg gatgtgtgtc caagtgtttt
    21061 tcgtaagaca aggaaagcta attgaacgag atgtttcaat gtttcctttc tataacgaag
    21121 cagaagaaga tttcttgact tacctcggtc agttttatca ggaaaatgag catttcattc
    21181 ctaaagaagt cttgattcct gatacgatcg acaagcccag tgtagaagct ttgttagcta
    21241 cgaaagtgct gcaaccaaaa cgaggagaaa agaaaaaatt agttgaattg gcgagtaaga
    21301 atgcctcagt tgctttgaat gaacggtttg atctgattgc cagaaaacag gaacggacga
    21361 tcggagcagt tgaacgttta ggaaacgcga tgaatatccc gaccccaata cgtatcgagt
    21421 cttttgataa ctctaatatc atggggacga atcctgtggc tgcgatggtc gtattcattg
    21481 atggaaaacc ggcaagaaaa gaatatcgga aatataaaat aaaaacagtc aacggaccgg
    21541 atgattatgc atcaatgaga gaagtcatct atcgcagata ttcgcgagtg attcgcgaag
    21601 ggttgccttt acctgatttg atcttgatcg atggaggaaa aggacaagta gatgtggcaa
    21661 aagatgtctt agaaaatcaa ttaggaatcg atattcctat tgctggcatg gtaaaaaacg
    21721 ataaacataa aacaaatgag ttgctattcg gtacagattt ggctgtagta ccgcttgagc
    21781 gaaattcacc agagtttttc ttactccagc gaatccaaga tgaagtccat cgattcgcaa
    21841 tcactttcca tcggcagcta cgcagtaaaa acagttttgc ttcccgatta gatgaaattg
    21901 aagggttagg accaaaacga aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata
    21961 tcacagcagc cacgacagaa gaattacaag aaatcggact gccgaaaaat gtcgcacaaa
    22021 acgtatttga taaattacat caggaatgag gaaagggcac tttacaaaga aagtaaagtg
    22081 tcctatcatt ttatgtacaa caaaaagagc gtttacgttt tgttggtaaa taataaatcg
    22141 cttacgtctg gtgtctaagg atatatgtga tttcttgaag aaggagtgaa gaaatgttag
    22201 aagtcaagga tcttgttaaa acttttggga actacacagc tgtcgatcat gtctctttcc
    22261 aaatccccga tggaaaaatc atggggctaa ttggccaaaa cggggctggg aagacaacga
    22321 cttttcgttt gatcttggat tttttgacac aagaccaagg agaagtgtta tggaatggcc
    22381 atcatcttgg ggagaaagat tatgatgtca tcggttatct tccggaggaa cggggacttt
    22441 atccgaaagt aacgattcaa gaccaactgc tttattttgc agaacttaga gggaaatcaa
    22501 gaaaagagat tgaaccaaaa atcgactttt ggatggaaaa atttcaggta aaagggaaaa
    22561 agacggataa agtaaaatct ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca
    22621 cgttaatcca tgaacctaaa ttgatcattt tggatgagcc attcagcggg ttagatccgg
    22681 taaatgctga attgctaaaa gatggaatat tggaattgaa aaaacatggt tcatgtgtca
    22741 ttttttctag ccacaatatg gataatgtag aaaaaatctg cgatcatttg atcatgttgc
    22801 gaaacggaaa aatggttttg aatggaaaag tacatgagat tcgtgaatca tttggccgta
    22861 cgaagttatt cttggaatct ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga
    22921 aaaaagtgat ccttcatgaa gacaatacgt tagagattac cttagaagat cctgaagctg
    22981 ggaaagaaat ctttactcgt gcgactcagt tcggctacat tcctatgttt aaccagcaac
    23041 cgccaacttt ggaagagatt ttcaaattga aagcaggtga aatcgatgcg taagttttgg
    23101 attattacca aagatgtcta tttaaaaaat gttaagtcga tttctttctt gatcatgata
    23161 ttagtccctt ttatcatgct gggtatcatt tatgtagcag gaaactttgc tcaacaaaat
    23221 agtgatgtca ataaaatcgg cgtattggct gataatgagc aaatcagtca gcaaatgagc
    23281 caagtcaaaa cagatgattt tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa
    23341 gagctttcgg aagaaaaaat cgatgcgtat ttagtagtct caactgagaa tgataagata
    23401 aatgggatgc tttatagcga aaattcatta ggacaaagta cacaactact gatacaacaa
    23461 caactgactg gcttgcaatc gatgcttcga gcgagtcaat taggcatttc accagaagaa
    23521 gtagctagct tgagccaaca agcaagtttg actcgacaaa aagtcaattt tgattcaaaa
    23581 ggaaaaatga cagtcggaga agataactcg acagtccaat acgtcgtaag ttatgtagca
    23641 acgattgtct tgttcattat tattttgacg tatgcgcaga tcattgcaca agagatcgct
    23701 tctgaaaaag gcacgcggat catggaagtg atcctgtcca gtacgactgc acaaaagcat
    23761 ttttacggaa aattaacagg tgttttactt gttgcattaa cgcaaatggc attatacggt
    23821 attatttttg gtataggata cagtcaattc aaagatatgg aaatggtcaa aaatatatta
    23881 gacggtatct cattggataa cttgattgga ccattcttgt ggtattctct tctattcatg
    23941 atctttggca ttttgatttt ttctgtttta gcagctttat gtggctcttt ggtcaacaaa
    24001 gcagaagata cagcaaaagc aattttgcca gtgacttatc tatctctagg cggatacatg
    24061 cttggattga tactaggagc ttcagatcca aacaatatcg ttattcggat cacttcgtat
    24121 attccattct tatcttcata tattatgccg attcgcttag caaatgagac agtcggtatg
    24181 ggtggagcat tgatttcctt gttggtactt gtagccgtaa cattcttatt gatgatcttg
    24241 tctgcaaaca tgtacaaatc gaatgtactc gtttatagcg aaggcggatt atggagttca
    24301 ttgaaacaat cgatttcaat tatgaggaat gaacgaaaaa aagcataaaa caatcctaaa
    24361 atacagaagg aagctggaca tctattgttc ccagcttctt tttgtataga cctattttat
    24421 ttattttact atattggata tatattcatt gaaaatagtg tataacaact ttataatatt
    24481 gagtatacca atttttggag gcgttttaat ggaccgttat ttagatttac tttcagaaaa
    24541 attcccctca acggaggatg tcatcacgga aatcatcaat ctcgaagcaa ttttgcagct
    24601 tccaaaagga acagagttat ttttgagtga tatacatggc gaatttcctg cttttgatca
    24661 tattctgcgt atcggttcag gaaatcttaa agaaaagatc aaagaccttt tttctgaaca
    24721 aatgacggaa acggatatca gccaattcac tatttttacg gcttatccag aatatgcatt
    24781 aacgactgat tggtacaaag aacaagataa atcgcagctg atccatcatt tgatcgatct
    24841 tttacggttt acgacagtca aatatacccg gtcaaaagtt cgaaaaggat tgccgaaaga
    24901 atatagctat atcattgagg aactcttgta tatcgatgac cgtatcaatg gaaaaaaaga
    24961 ctatgtaaaa aaaatcattg aacaacttgt tttgatgaag gaagaagaaa aatttttaac
    25021 gaaacttgca cagacgatcc aaaaatctgt catcgatcat ttgcatatcg ttggagatat
    25081 ttttgaccga ggaactcaag cagccaaagt gatggatcga atcatggatt tttcttctgt
    25141 agatatccaa tggggaaatc atgatatttt gtggattggt gcgtattgcg gctctgaggc
    25201 atgtttactg acacttttac ggattgcagc acgctataat tatttatttg aattagagaa
    25261 agaatatggg ttgaatttac gtccattgtt ttcttttgct catcaaactt atcagaagaa
    25321 tccagtattt gcgcctaaaa acagtaaaaa tctatccgaa agagagcaag aagaattaga
    25381 aaaaatccat caggcacttt ctatccttca attcaaatca gaacatcagt tattggatcg
    25441 tcgacctgaa tttaaaatgg acgatcgtaa attgctggaa aaaatcgact atgaagcatc
    25501 aacgattgat ttagaaggtc agctctatga gttgaaagga acttgttttc aaacgatcca
    25561 gccagatgat ccaaaaaaat tcttggaaga ggaacaaaaa gtgattgata gtttgatgta
    25621 ttcattccag cactctttga gaatgcaaaa acatatgacg tttttgatcg aaaaaggggg
    25681 aatgtatctc acgaacaatc agcatgtgct gtttcatggc tgtatacctg tagacgagaa
    25741 gggacagctc ttagcatttg aattagacca atcttatcgc ggaaaagaac tgctgggctt
    25801 ttttgaaaaa cagataactg aaagcgcaaa agatctgact gcaaaagaag atttagcaac
    25861 cgatttgatt tggtatgcgt ggagtgggcc attttcgcct ttattcggca agagtaaaat
    25921 ggcgactttt gaacgttatt ttctcacaga gcctcatacg cacgtcgaga aaagcaatcc
    25981 ttattatcac ttgcgagatg aagagtggtt cagtgaaaaa attttagcag aattcgacac
    26041 aaaagaagaa gggtcagcaa tcattaatgg gcacacgcca gtaaaagtaa aaaaagggga
    26101 gtcaccgatc aaagcaaatg gaaaagtatt tgtcattgac ggaggtttat ccaaagctta
    26161 tcaaaaaacg acaggaattg ccggttattc actattatgc aattcttatg gtttccaaat
    26221 cgtgacacat tatccgtttt tgcctattga acaacaattt gcaaaaaaat ccgaccaaac
    26281 aaatgtgaaa aaagtaatcg aacagcaact accacgcaaa ctcaatcgtg atacaacaaa
    26341 aggtatggaa ctgcagcagc agattaccca gctcaaaagg ctgttggact atcgaaagga
    26401 cgattgagaa gaattccagt gaacattgtt ccatagactg gagactgaga caaaagtcgt
    26461 gtcgcagtct ctttttccgg ataaacggtg ttcttctgcc aactcctcgg tcacaagtca
    26521 cactattcaa caaacatgaa aagctgtttt ttgaaattgc tccttgtgac tcagagttga
    26581 acggcaggct cacgacctct tggtataaac ggtgttcaaa agccagtttc tcggtattaa
    26641 gccaaattta tgcaaaaatg tgaaaagcca ttttcacaaa atttttctta atactcgaaa
    26701 ctaaacagct ttttcacaac cccctcagaa agtctattaa aattcattca caaagaaatc
    26761 gaataagcgc tgttcagatg gatgtacacg gtgggtaagt tccggatgcc attgaacacc
    26821 gaggatttta tgcatatcat ctttgctttg aaccgcttca acgagtccat cgttagaaaa
    26881 agcgatggct tttaataaag gtgaaagatc tttgactgcc tgatgatggt aagaattcac
    26941 aaaatatgga tcggggagaa ggttgcttaa caaactgtct gaaacaatct tgacttcgtg
    27001 agtcgtaaat tgcggggcag tgggctgttg ttcatgtttt actgaccact ttggatataa
    27061 tgaaagatct tgataaagtg taccttccaa aacgacattc aatagttgga gtccccggca
    27121 gactgcaaaa attggttttc tttgtttcaa tgcttccaag atcagtgcct gttcaaattg
    27181 gtctcgttga atatttgttt ccgttagctt tggatgagga tcttccatgt aaagttgtgg
    27241 actgacatct tgaccacctg caagaataag tttgtcgatt tgtccaatca gttgaggagc
    27301 tgaatctgga gacatgactg gcagaataag aggcaatcct tgagcatcta aaactgcatc
    27361 cacgaatcct tgcggtgtat agctcacttg atttccttgg aaagtatctg ttgctcgaat
    27421 cagttgattt ccggcaatgc cgatgattgg tttcatttgt attcccccta aagtagactt
    27481 attgtaacaa ggatttaaaa aaaattaaac tatgtaagac ttgcccctat acttatctgt
    27541 tcttgctgtg ttactatctg taaagagaaa tttcgtgtga aattcatgac gatagttgaa
    27601 aatattgctt aatttgttat aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct
    27661 tcaaggatct agttcttcaa ggggtgagaa ggacatttta ttgaaggggc ctgcctatac
    27721 taagttatct ttagaggtac atactttcaa tgaaaaaaga aagcatgtac ctctttttta
    27781 aatcgaaagg aagtgaagca agtgttagaa gctttggaac aaatccgaaa agcagaagaa
    27841 gaaaatgaaa agcgtgctca aacactgcgg cagcagctga agatctatgc aacagaaaaa
    27901 gagcaggaat tggaaacgat ccaaacaaac tatcgacaac aactaaaaca gttggtggaa
    27961 gaaaaagaac aatttgaaat acaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa
    28021 gcagacaagg tgcaagcaac tttgaaagat caatatcaaa agcatcagag tcaagcgatc
    28081 ggtgcgatca ttgaaagggt gaaagaaact tatggccgtc actaagatgg aaaaagtgac
    28141 cctcatttca gataaaagaa atcaggagag gattttacaa gcagtacaag gaatccaaaa
    28201 cgttgaaatc cgggatctgt atcaaaatag aacaaacaac cagtgggtgg agaagtattt
    28261 tccagatacc acgattattg ataaagaagc aaaattaagc gctttaggta atcggttgaa
    28321 agacatccaa gaagctatcc aattcataca acaccacggc gatagccgtc aaaagaaact
    28381 ccatttgaaa cgtcaagaac tttccttaca agatttggaa gccaattatt ccgaagaaaa
    28441 tttcataaat aaattgaaag aagtccttga tttaaaagaa aagtgggaac aactaactga
    28501 agaacgtgat cgtttagaag aagaggaaga atggcttctt cattggcaac agctggatat
    28561 tgctccaagt atgtatgcaa gtaagacgac catttttgag atggggactg tgagtgcagc
    28621 aaattttgaa agtttcaagg aagaactgag taagcttgat gacgtttata tggaagaaat
    28681 agattatacg ccaaaacaag tttatctttc gtatgttgct ttaaaaaaat cagttgctgt
    28741 tatagaagaa attgctggga aatacgggtt caataaagaa acgtatcctt atgaacaaaa
    28801 tcctaaagaa aaactgcaag agaccaagca gcaattatcc attgttcacg agcagcaaaa
    28861 gaagataacg acggcgttag gtcagtgcag tggctatatc caagaatttg aatgggtaga
    28921 agaagtcaca cttgccgttg cagaacgaga aaaaataaaa gaacggttta tccatgcgac
    28981 ttatctgatc gttttgcaag gttgggtaga tgcagaagaa aaacagaatt tgctgcatgt
    29041 gttaaacgaa accgtatcag aagaagatat atacgtcagt ttcgaaacac caaactcaga
    29101 agagattcag caggaagtcc ctactaaatt gaaaaaccat ccgcttgtcg aaccttttga
    29161 attgctgact gaaatgtaca gtctgccaaa atatgaagaa gtagatccaa caccttggat
    29221 gacaccattt tatcttgtct tcttcgggat gatggtcgca gatgttggtt atggtttgct
    29281 gatgctcatt gggacaatac tagctcaaaa actacttgtt ctaccacgcg ggatgaaacg
    29341 tttcgtaaaa ttctttgaga ttctctcgat accatcgatc atctggggat tgatctacag
    29401 ttcatttttc ggacagtcat taccaaaaga actgtttggc atccgcttgc ctttcccaat
    29461 cctatctacg acggatgacg tcaatacgat tttgatttta tccgtgattt tcggtctgat
    29521 acagattttg gttggattat ttgtggcagc aaaggaaaat atcaggagaa aagattactt
    29581 gagtgctgtc agtgatggat ttgcctggca agggatccta attgggatcg ttattgcgtt
    29641 ggttggtgcc atgttgataa aaaatacagc atttgtttac cttggcgctg ggctatcgat
    29701 cattgctgcc ttatgtatct tgatcgttcc gattatccaa acgccgtcta aagtcaaagg
    29761 tgctgccaaa ggtgcgtata atctttacgg actgaccggc tatatcggtg atttagtcag
    29821 ttatacacgt ttgatggcat tgggtatttc aggcggaagt attggagcgg cattcaatat
    29881 gctagttgct ttcatgccgc cagctgctcg attcagtgta gggatccttt tgatcatcgc
    29941 tttacacgca ctgaatatgt ttttgacact gctaagtgcc tatgtccatg gcgcacgatt
    30001 gcagtatgtc gaattttttg ggaagtttta tacaggaggc ggacgtgcct ttgatccgct
    30061 gaaaacggcg gaaaaatacg tcaacatcaa tcacaagaaa aaaaacaata aataattgga
    30121 ggatttttat ataatgatgg attacttaat tagtcaaaat ggtggaatgg tatttgcggt
    30181 attagcgatg gcaacagcga cgattttttc agggatcggt tcagctaaag gtgttggaat
    30241 gacaggtgag gcagcagcag cattgacaac aagtcaaccc gaaaaattcg ggcaggctct
    30301 gattctgcaa ttgcttccag gcacgcaagg tttgtatgga ttcgttattg cctttttgat
    30361 ttttatcaac ttaggaaatg acatgtcagt tgtacaagga ctgaactttc taggtgcttc
    30421 tctgccgatt gcttttacag gcttgttttc aggtattgcc caaggaaaag tcgcagcggc
    30481 aggtattcaa attttagcga aaaaacctga acatgctaca aaaggaatca tttttgctgc
    30541 gatggttgaa acatacgcga ttttaggttt cgttatttcc ttcctattag tgttgaatgc
    30601 ctaagcataa aggaggaaaa gcagatgaac gccattgaaa aaatcatctc tcagatgaac
    30661 gaagcagcag agcaggaaag agcggcgtta gaacaagaag agcgaatgaa gatcgatcaa
    30721 aactttgaac aaaaacgtac gcaagttgaa acagaacatc aaaaacaaaa agaaaaacaa
    30781 atcgaattac tcgaaaaaaa ataccgtcag ctaaggaatc gccagcaagt agaagtacga
    30841 caagaaaatt tgaatgcgaa gcaggaattt ttaagacgat tgtttgcaga tgcggtaact
    30901 gaaatggaaa attgggatga atctgaacaa atccagttta tcaaaaatgc attgtattca
    30961 ttacctttga caggaaaagt agcattcatt gctggagaaa aatcagcagc ctatctttct
    31021 cagacgttgt tggatgaatg gaacaatgaa cttcccttta tgatggtttt aagtgatgaa
    31081 actgttgctg atcaagcggg ttttctgata aacgatcaag gagttcagta taattttctt
    31141 ttcagcagcc ttgtacaaga tatccaagga acgatgagtt ttgagattgc caatcaatta
    31201 ttcgaataag gagggatcag tgtatggaat accacgaact aaatcccttg attcgcggaa
    31261 gagagcttga acttttgtcc aatgagacat ttgaacggat gattcaaaca aattctgtcg
    31321 aagcacttgg tgaaatattg aaatccacga tttacagtcc atatatttat gacggatatg
    31381 aatatgactt cgaacagaat cttttaaagg agcagagccg tctatttcgc tggttaaagg
    31441 aatcagcacc ggagcctgaa atcgtttgga tctatacgat gcgttatact ttccataatc
    31501 tgaaggtttt gacaaaagct gagatgacaa atcaaaatct ggaccatttg tatatcgacg
    31561 atggatttta ttcattagaa accttgaagg atgccattca tacgcaagtt tcaaatgaac
    31621 ttcctgaaag cattatggac agtatacgag aagtacgtga ttattttgaa gagtctacca
    31681 ttcttcaggg aattgacgtg atttatgatc gacagttttt gacagagcag cgacggcttg
    31741 ccgataagct aggatatcaa gaattgctga cagaagtcat tgcttttatt gacttgacca
    31801 atatcacaac gatggctcga ggaatattgc agaaaagaac atatgggttc atgacgacgg
    31861 tactttccag ctcgggaagt atcgaaaaag aagtattttt gaactttgta gataaagata
    31921 tggaagatta cacgaatttt ctattagaga cagattatgc tgattttctg cgccctgtca
    31981 tccgtgaggg aaatattgat ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt
    32041 cattgtatca agaagcacaa acccaggctt tcggaccact gccactacta gcctttttga
    32101 acgcaaaaga ggtagaaagc aaaaaccttc gtttactggt tatcggcaaa agaagccatt
    32161 tcgaatcaga aacaatcaga aagcgggtga gacaagtcta tgacgcataa gatcggtgta
    32221 gttggcgaca aagattctgt tttgccattc aaattgtttg gctttgatgt tcgttatggt
    32281 acaaccaagc aaaccgttcg tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg
    32341 atttatgtca cagagcaatg tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa
    32401 caaatgatac ctgctatcgt attgattccc aatcatcaag gatcattagg gatagggctg
    32461 gaagaaattc aaaaaaacgt agaaaaagca gtaggtcaaa atatcttata gtatgaaaag
    32521 gagatagaaa ttttgcaaat tggaaaaatc ataaaagtct ccggacctct tgtaatggct
    32581 gagaacatgt ctgatgccag tatccaagac atgtgtctag tgggagatct aggagttatc
    32641 ggagaaatta tcgagatgcg tggcgatgtg gcgtctattc aagtatatga agagacttct
    32701 ggaattggtc caggcgaacc ggttcgctct actggtgaag ctttatctgt tgaattagga
    32761 ccagggatca tttcacaaat gtttgacgga atccaacggc cattagatac ctttatggaa
    32821 atcacacaaa gtaatttctt agggcgcgga gttcaattgc ccgcattgga ccatgagaaa
    32881 aaatggtggt tcgaaccaac agtagaagcc ggagaaacgg tgagtgctgg agatgttatc
    32941 gggatcgtag aagaaacaaa agttataaaa cataaaatca tggtgccaaa tgggataaaa
    33001 ggaacgatca aaacaatcga aaaaggttca ttcactattg atgaaacagt atgtgtcatt
    33061 gaaaccgaaa atggtgacaa aaatttgacg atgcttcaaa aatggccagt aagacgtagt
    33121 cggccaatca aagaaaaatt aaacccggat gcacccatga tcactggaca gcgagtgatt
    33181 gatacattct tcccagtgac aaaaggagga gcagcagctg tacctggtcc ttttggtgca
    33241 gggaaaacag tcgttcagca tcaaattgca aaatgggccg atgtggacat ggttgtctat
    33301 gtgggatgtg gcgagcgtgg aaatgagatg acggatgttt taaatgaatt ccctgaattg
    33361 attgacccta atacaggaga atcgttgatg gaacgtacgg tattgattgc taatacatca
    33421 aacatgccag tagcagctcg agaagcttct atctatacgg gaatcacaat tgcggaatat
    33481 ttccgtgaca tgggttatga cgttgctatc atggcagact ccacttcacg atgggcagaa
    33541 gcacttcgtg agatgagcgg acgtttggaa gaaatgcctg gtgatgaagg ttatccagct
    33601 tatcttggtt cacggcttgc ggaatactat gaacgatctg gaagagtcat tgcattagga
    33661 tcagaaggtc gcgaaggaag tataaccgca atcagtgctg tatccccgtc tggaggggat
    33721 atttcggaac cagtcacaca aaatacattg cgggtcgtaa aagtattctg gggtcttgat
    33781 tccagtttag cccaaaaaag acattttcct tcgatcgatt ggatacaaag ctattctctt
    33841 tattcaacag aagtagggaa gtatatggat catatccttc aagctgactg ggcatccatg
    33901 gtcaccgagg gcatgcgtat tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg
    33961 gtagggattg attccctttc agataatgac cgtctgacat tagaagtggc aaaatccatt
    34021 cgtgaagatt atttgcagca aaatgctttt gatgatgtcg atacattcac ttcaagagag
    34081 aaacaattca aaatgttgaa gatgatcttg acctttggtg aagaaggacg ccgtgcgttg
    34141 tcgttaggtg cttatttaag tgagatcatg gacggtaccg tatcgattcg tgaaaggatc
    34201 ggacgaagca aatatatccc ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa
    34261 atcaagcaaa cgatgcaaac gatcgtttca gaaggaggca tgagcaatga ttaaagaata
    34321 tcgtacgatc ggtgaagtcg tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa
    34381 atatgaagaa ttaatcgaag tccgtatgca aaatggcgag attcgtcgtg gacaagtatt
    34441 ggaagtccaa gaagataaag caatggtcca aatattcgaa ggaacaagtg gtatcaattt
    34501 acgtgactct tctgtacgat ttttaggcca tcctttagaa ctaggtgtat cggaagacat
    34561 gatcggtcga gtattcgatg gtttgggacg accgaaagat aacggaccag agatcctgcc
    34621 tgaaaagcat ttggatatta acggagaagt aatcaatcca gtagctcggg attatccaga
    34681 tgagtttatc caaacaggta tctctgcaat cgatcatttg aatacattag ttcgtgggca
    34741 aaaattacca gtattttctg gctctggtct tcctcataaa gaattagctg ctcagatcgc
    34801 gcgacaagca acggtactcg attcatcaga tgatttcgcc gtagtctttg ctgctattgg
    34861 aatcactttt gaagaagctg aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga
    34921 ccgttctgtc atgtttatga atttagcaaa cgaccctgca atcgaacgta tcgctactcc
    34981 tcgtatggct ttgactgctg cagaatatct tgcttatgaa aagggaatgc atgttttggt
    35041 tatcatgaca gacatgacca actatgcaga ggcattgcga gagatttctg cagcacggcg
    35101 agaagttcca ggaagaagag gatatcctgg gtatctttac acgaacttag ctactttatt
    35161 cgaacgagca ggaagaatcc gcggactaaa aggatcggtt actcaaattc cgattctaac
    35221 aatgccagaa gacgataaaa ctcatccaat ccctgacttg actggttata ttacagaagg
    35281 acagatcatc ttgactagag agctttacaa gagtgggatt cagccaccaa ttgatgtact
    35341 cccgtctctt tcgcggttaa aagataaggg aactggaccc ggaaagacaa gagaagacca
    35401 tgcagctacg atgaatcagc tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt
    35461 agccgtagtt ttaggtgaat ctgctttatc tgatgtagac aagatctatg cgaagtttgc
    35521 tgagcggttt gaaaaagaat atgtcaatca aggattctac acgaaccgtt cgattactga
    35581 aacacttgat ctagggtggg aactgctatc gatgttgcca agaaccgaat taaaacgaat
    35641 caaagacgac atgctggaca aatatctgcc agaagggaag tgagttctga tggcacgatt
    35701 gaatgtgaat ccgacccgca tggaattgac tcgattgaaa aaacagttga caactgccac
    35761 aagagggcat aaattattaa aagataagca agatgaactg atgcgtcaat tcatcctgtt
    35821 gattcgaaag aacaaccagc tgcgtcaaga aatggaagag tctatgacag aagcaatgtc
    35881 tgattttgta ttggcaaatg cttcgatcac ggaacctttc atcgaagaat tatttgtttt
    35941 gccggcggaa aacgttgatt tgtctatcgt agacaaaaat atcatgagtg taaaagtacc
    36001 tgtgatgaat tttgaatatg atgaacagct gacagacaca cctttggaat acgggtattt
    36061 gaattccagt gccgaactgg atcggtcaat cgatcggttt actaccctgc ttccaaaact
    36121 tttggaactg acagaaatcg aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac
    36181 acgtcggcga gtcaatgcat tagaatatat gacgatccct caattagaag aaacgatcta
    36241 ttatattcgt atgaagctgg aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt
    36301 aaaaaatatg ggtaccactg actaagtggt atcttttttt aaaagaggaa aacgatgatt
    36361 aaacaaagca aacaaataaa gaaacgtttg ccggctgttc agcttctcgc attaggattt
    36421 tttctcctga ttttagttgg cggcggccta cttaccctac cattttttag taatagtggg
    36481 catgggacaa attttatcga tgcgcttttc actgcaactt ctgctgtctg tgtaacggga
    36541 ttgacaacat tgaatacagc agagcattgg aacagtgctg gtcaattttt gatcatgctt
    36601 ttgattgaga tcggcggtct tggttttatg atggttccga ttctatgctt tgcattgata
    36661 aagaagaaag tcagtctcag cacacgtatt atattaaaag aagcgctgaa tctagaagaa
    36721 gtatcaggtg tgatgaagct catgctttac atcctaaaat tggcctttgt gatccaattg
    36781 attggtacgg ttgctttatc ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc
    36841 tggtacagtt tgttccatgc aatctctagt ttttgtaatg cagggtttga tttgttagga
    36901 gacagtttag ccaatcacca gcaaaatgtt tatttgatca tggtggtatc tgctttgatc
    36961 atttctggtg ggttaggctt catcgtatgg cgtgatttac tggaatatca tcagaaaaag
    37021 aagatgacga ttcattcaaa aatcgcatta accgttacag gtaccctttt attaggcgga
    37081 ttcgttgtgt tctttttcac agagcgcaat ggggcaacgc tagtggaagg gaatatgttt
    37141 caacgattgg caaacacctt ttttatgagt gtgacaccta gaacggctgg atattattca
    37201 gttgattacc tccatatgag ccatgcaggt ctgattttaa cgatgttttt gatgtacatc
    37261 ggcggaacat ctgggtcgac ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg
    37321 atccagatgc gttcgatgtt gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc
    37381 cgacaaggcg cagtgttgcg ggcacttact ttattttttg ttacgttgac gttgtgtgta
    37441 gtcgccatca tgattttatc ggtgactgaa acgataccta aaacttctgg catcgaatat
    37501 attgcttttg aggtcttctc tgcgtttggt acagtaggtc ttacgatggg attgacgcca
    37561 gacttgactg caatagggaa attgatcatt atagcgctta tgtacattgg acgagttggt
    37621 gtattgactg tggtcttctc tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa
    37681 tatccagaag aaagtgtcat gattggataa tttcatcttc tgacagaaac ctagtgattt
    37741 agtaaaataa gaaaataaaa taatttttct tgcttcttgg atactttaag tgcaaaatgt
    37801 tcgtttattt tttgaggcac tacccgttgt tgaacctcgg gtagtgcctc ttttttattg
    37861 aaaggaatca ataagatttg attcctttat ttatcaatag gttataattc atattttttt
    37921 attttttctt ttacgatact tagtccagaa gctaaaattt caaggttatt agaggatagg
    37981 gaaattcgaa cgaatgcatc tggttgcgca cgtgtagtaa agcgtctgga atgataaagt
    38041 cggataccgt tagcaagaaa gtcattctca attatcttag ggtcaatctc aggatcaact
    38101 ggaagaattc gatagtaagg gacacgattc gttggttttt ctactttgaa aagagaatca
    38161 aaaagctgat tggctctttt tgtaagactt aatttagttt ctaagatcct atttgcttca
    38221 tttgaatgga ggatttgagt gatgacttcc acatctaatc cagaagtttt cacatttata
    38281 ttaaaaattg cttgttctat cttgctttgg acatgtactg gaactacaag ataagcaact
    38341 cgcaatccag aacaaataaa ttttgtcatt ccagctaagt agattgtttg gttaggtaat
    38401 aaattttgga aaggaggaat gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata
    38461 tcatcttcaa taacccatag accttctttt tcaatcactt cggctaattg ctctcgtctt
    38521 ttgtgagaga tttgaaaccc tataggatta ttacaagaag gcataagaaa gacaccatga
    38581 atcttctttt ttctacattc ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa
    38641 tcaattggaa cgatctctaa atgatataac tgagccaatt caatgaaatt agcgtatgta
    38701 tatcggtctg ttgctattct atcacctgga gagaaaagtg cagaaagtgt gatagccaat
    38761 ccattttgaa caccggaaac aatagataga gattctaagg acggaatgac tccttgagtt
    38821 tttaaccaat ttgctgcagt agtcaattga aaatttgtgc ctttgggatc ttggtaatta
    38881 agcaaaccaa caagatttgg gttattacta gttgattgaa taaacgggac aacgaattta
    38941 ttacattctt caaaagaact aaccagacca aaatcaatga tatgttcggt tacttcattg
    39001 gtagagatgg tgatggactc aaagacattg ggagaaataa atgtgccact tccaatattc
    39061 gtatacagca aacctttctc aattccatat ttataagcct tgccaacggt agtaaaattt
    39121 aaatctaaat aatcagctag ctctctttgg gagggtaact gagtgttttt ctgtaattta
    39181 ttctttaaaa tatcttgttc taattgttga atcaacgatc gataataagg acgcttcaat
    39241 ttttgtttgt ctggtcgcca agttaacggg taattgtcat aagaattaat aggcattttc
    39301 tccactcctt aaaattgtat accatacaat tttaccattg attgtattga ttttagaagt
    39361 taatcttttg tgaggaggga attgtctatg aaaattatta attttgagga agtagtagct
    39421 tcttttagtt ttgaagaagc gattcaagta atgaaaaaat gtttttttga ttatgaaagt
    39481 ggtaaaatca gccaaaatcc tcgattggtg gaaattttac ctgacggaaa aggcaaaaat
    39541 atgtttgcga tgatgcctgc ttatttgggg gagaacagat attttggcac aaaagtcatt
    39601 accgcttttc cggataatca tcagtatcat ttaccttctc atttaggaga gattttcttg
    39661 tttgatgctc aaaacggact acccaaagca ttgataaacg caaatgcagt cacctggatt
    39721 cgaacagctg ctgtatctgc gctggcaaca gattacttag caaatccgca tgcagaaaca
    39781 cttgctttaa ttggagctgg tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt
    39841 cgtcccatca ctacagtgtt tgtttacgat attttagaag aaagacgaaa tacttttatc
    39901 gctaaagcta aaaaacagta tcctaaaata acgtttataa atgcaatatc agtagaagat
    39961 gcagtacgtc cagcggaaat catttgtacg ttgacctcta gtaaagaggc atttcttcaa
    40021 aaagagtggg taagggaaga cgcacatatc aatgctgtgg gcacatttac tcctgataca
    40081 agagagataa gtacatcgtt gatggaaact tctcaaatct atgtagatga ttactcagct
    40141 gccttaacag aaagcgggga cctgcttata gccatggcag aaggagattt agaggaatct
    40201 tgtattgttg gtacattagg cgaattagtt tctgagaaaa aaacatttta taaaggagaa
    40261 tcgaggccga cgattttcga cgcagttggt ctagctattg aagatctttg ttgtgcagaa
    40321 tatatttata acaaattaga gaaaggggct ttaaaatgaa cttagattat tacgtagttg
    40381 atgcatttgc agatgaggtg ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat
    40441 ggctgccaga aggtacgatg caaaaaattg ccattgaaaa caatttatct gaaacagcat
    40501 ttacagtgaa gaaaaaccaa gaatttgagc ttagatggtt tacgccagat cgagaaatcg
    40561 atctttgtgg tcacgcgaca ttagcaacag catttgtttt atttaactat tacaaaatac
    40621 ctgatgaaac gattaaattt tctactcaaa gcggtaatct atttgtgaca agaattaaag
    40681 attattatta catggatttt cctagtatta tgccgaaaaa agtgccgata ttgtctgaat
    40741 atgaagaagc gattggggct aagattaaag aagcctactt agcacgtgat ttatttttcg
    40801 tattagaaga tgaaaaaacg gtggctaaat tgcagccaga ttttacagct atcaaaaatt
    40861 ttgaattagg aataggagtc atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta
    40921 gaaccttctt tccaaaatta acaatcaatg aagatccagt atgtggatca gcacattcta
    40981 atcttattcc ttattgggca gaaagaatga ataaaacaaa attgtcagct tatcaaatgt
    41041 catcaagggg cggggatcta gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa
    41101 aagcagtttt gttttctaaa ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa
    41161 taaattggtg gagagtggca accttatagc tacaatggtt gttaaaaaaa gacgcaaact
    41221 ttttcaattt aatgaaaagt ttgcgtctca ttttaattat tggtgtttgt tttttattca
    41281 atcaagagta aaatgcccca tactaactat cctcggatga cttcgatttt ataaccatca
    41341 gggtcaatga caaagtagta agaaggcgca gtgccaggta atccttttaa gtcagtaatc
    41401 tcaagaccag cagctttatg ttgctcgtgt aatttttcta gatcatcagc tgctatagcg
    41461 atatgaccat agccgtctcc tagatcatac gcaccatgat catagttgta agtcaactct
    41521 aattcatagt catccccagg aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa
    41581 tcacggcggc ggctttcttc aaaaccaaat gccttttgat aaaattcgat tgatgcttca
    41641 agatctttca ctcgaacaca tgtgtgagcc attttcatag attcatctct ccttttcctt
    41701 ttactcaatc ataacaaaaa gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa
    41761 tttctcatgt aaaatggata gaaaataaaa ctgttaaggt ggaaactatt catggataaa
    41821 ccaatttttg gtgaaaaaaa gcaatttgct gattatcaaa cccgttatgc agcatatatt
    41881 gtcatagaaa gagatcagca gattgcagta atcgaggctc caaatggtgc tttttttctt
    41941 cctggcgggg aaattgaagg agaagagacg aaagaagaag cgattgtccg tgagctgata
    42001 gaagaaatgg gaattgtggc agaaatttct tcttatctgg gagaagctga tgagtatttt
    42061 tactcgaatt atcgtgcaac ttattattat aatccaggat atttctttgt tgctgattct
    42121 tggaagcgta taggagaacc aactgaaaag accaataaaa tttggtgggt gagccctcaa
    42181 gaagcgcttg tgaaattgaa acgtggtagc caccgatggg cagtcgagag atggatcgaa
    42241 aaaaataaaa actgaaagaa agcgagtaga tcagctttct ctcagttttt ttatttatta
    42301 cagtacatat tttttgattg cttcagctac accgtgctcg tcgtttgatg aagtgatgac
    42361 atttgctgct tctttgacaa gagaaacggc atttcccatt gctactccta gaccagcata
    42421 ttcaatcatg gataagtcgt tctcgttatc tccgatagcc atgatttcat cttgatcaat
    42481 accaaggtgc tgtgcgagat tcgcaactgc cgcaccttta cttgcttctt tatttagtac
    42541 ttcgaagtaa tagggcgcac ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa
    42601 acgcgcaatt gctgcatcta aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt
    42661 catgtctggt gtcatttctt caggtgttcg atattttagc ggcatcttga ctaaacttgc
    42721 ttcgtggatc gtataagcgc tgatatctcg attaggcgtg tagatcgctt tgtcatcgat
    42781 cgaatggaga tgactgccga cacggcgtgc cattacttcg atctctagga aatcttcatg
    42841 tgtcagtccg tgacgggcga tgattttacc tgatttcgtt tgttgtacga gtgccccgtt
    42901 ataagtaata acgtaatcat tatcttgaaa aaggttcaac tcgtttaact gttcctggac
    42961 acctggtaag ggacggccag tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc
    43021 aatcgcttct tttacttctt ttgttacttc tcttttctca tttaataaag taccatcgat
    43081 gtcgatagcg actagtttga tagacataat ttcctccaat atattttaat ttatttcaat
    43141 caatgcgcca ttacggatat ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata
    43201 atgatcacgc aaggacggat caaccatttc ttttggaaag aaaaatcgct catctccttg
    43261 ttcttgtccc gctaatgcag caacaagctg actaacctgt gataattcta tcaaagttcc
    43321 gtcatttcgc aaaatctcga tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc
    43381 ataaggtaga tcgtagctag aattcacagc agtgtaataa acaggattat atccgacctt
    43441 ttctactaac aaagtgagtt cttggactaa ttttgaatcc cggttgccag aaaaagtagc
    43501 agatttcagt ggtttacgat tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc
    43561 agattcagac cattgagtaa agtaagtgcc caagacccca tcatcaagtt tgaggtattc
    43621 ttctaaagta aagcttccgt caagaaatgg cagaagtaat tgagaatgga gttcaaaact
    43681 gctggcatct tcttgataca actcgtgtgc gcgatgaagc agatgctcca aaattacttc
    43741 cataccacgg gaaacaggat ggaagtacac ttgcacatac atttgatatc ggctaacgat
    43801 ataatcttcc actgcatgca tgccactcat ggaaaaagcg atcccgcctt cataaggacg
    43861 aatcacacgt aatattcggg taagatcaaa tgttccatat tctgttccag tgaaataagc
    43921 atcccgaagc aaatagtcca ttcgatctgc atcgatctga ctagaaatca tttggacaac
    43981 ttgtgggttg gggtaagttt tttggatcac actagctacc ttttcaggaa aaccttcttc
    44041 tactcggtta agaatctgat acacttctgt ttctggagaa gtgatgatct ctacagtgat
    44101 ggcttcatga ttcgtatgga agatatgttc aaatgtatga gagtaaggac catgaccgac
    44161 atcatgcaac agtgccgcac ataaagtcac cagccgttcc tgatcatccc agcctccgtt
    44221 gcctattttt tcttttgaaa aatttcgaga aaagatatcg cagattcgtc ttgaaatctc
    44281 ataaacgcct aatgaatgag taaaacggct atgttctgct ccgtgaaaag tgaaagaaga
    44341 agtgcctaat tgtttaatgc gtcgtagacg ttggacttct ttcgaattga tcaaatctaa
    44401 gattacttga tgctggacat ggatatagtt atggactgga tcacgaaaaa ctttttccat
    44461 aggcagcatt tgatatttat aaggggttgt cataatgtag ctcctttatg ttattacgtt
    44521 gttccattct ctggatttgg ttgttccatt gctccagctg gatttctttt tttagccagt
    44581 cggtagaatc ctgtaaatca cttgcagaat acagcttttc aaaagctttt gcaaatgctt
    44641 ctttgacttc ttttacaaaa aggggttggt caagcagttc ttcaagtgtt gccattgtag
    44701 caggatccac tgcaggatag ccatttgtac caaagtcttc ttttaaagca gattgataaa
    44761 atcgcttcat gacttgccca cgtttttcct gattaccgtt gacacttaag tacatcataa
    44821 cagaaatccc atttttgatc cgacgctgag cgataccggc aaattttttg ccatggatgc
    44881 ttaaatcata agttccagga caataagaat gggtgatttc gaatgcttgt atcggtgcct
    44941 ttggaaaagc gtatttagtc aataggagca tttgctcgta agcgtcatct atggttattt
    45001 tttttgtgtc actttggggg aaaatcaaag aaatattcaa aattccttca tctgatacga
    45061 tccctaatcc acctgaattt ctgatcactg gagcgtaacc tgcatcttcc agtgtttgga
    45121 tcccgtcact tagaaaagga gttcgaacat ccttcatacc taagatgact gtgcagtcat
    45181 attgccagaa atgtaaaaag atttgctggt gttctttaga atattccgtt aatatatctg
    45241 ttaatgcaaa aggggaaaag tcgttttttt gataaatgtg ctgatctagt cgaatcgcag
    45301 ggttcattca accggctcct ttgtataaat tcatattttc tacaatatta tactctaaaa
    45361 tcaaagagtt gttttggaaa aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac
    45421 aaaagaaact aggaacaatg tagctatgag aagtaaagga gaatgaataa tggaattatc
    45481 acaaggaaca ccgatttcaa tcaaattgcg tacaaaagta aaacagcaag gggaagtgca
    45541 agatttttat tttgatttga aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg
    45601 ttataaagag attcaggagg agacgggcga tgagattcct gtaacgatca aattagttcc
    45661 agatggtcat gtacagctga tccgtgcagg agaaatgcgg atgcgtttga aatttggcta
    45721 taaagaacgg ttagaaacat cctatcgaac accatacggt atgatccaga ttgctacttt
    45781 tacgaaagaa ctgcatgtta gtttgaaaga ccgtcccaca gcagggaagg ttcgaatcga
    45841 ttatgatctg ttcatgggac cggaaaaaat cggcgaatat tatttgacct tggattttac
    45901 agcgtaaata taaaagaaaa gtttgatttt ttgtgtctga ttttgtatga taaggagtag
    45961 actgtgaaag gacgtgtcct gattggaaat tagtgtattt gaaggtgcaa acaaaagcga
    46021 attatcaatg atcgaagtag cacacgcgat cttagaacaa cgtggcgatg tcatggattt
    46081 ttctgattta gctaaccaga tccaaaacta tcttgggaaa tcagacagtg aaattcgtga
    46141 ttcactcgca caattttata ctgacttgaa tattgacggc agctttatct cattagggga
    46201 taatcgttgg ggcttgcgtt catggtatgc tatcgattcg atcgatgaag aagtaaacca
    46261 tggaatggac gaagaggatg aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc
    46321 ctttatcaat gatgatgaag acgcgatcga ttacaatgac gatgacccag aagatacaga
    46381 tctgacagaa gaagatgacg atgatctgtt tgatgacgat gatgatgaag aagacttgcc
    46441 aggaaatatc gaagaagatt taagtatcat agacgaagac gatgatgatg aagattatga
    46501 agaggaagaa ttttctgact ctgaaaaaga ataaacatta ttttcttacg agaggttgtg
    46561 acagagacga tcagcctatc agaataagga ggtgtgacac gatttttgtc acaccttctt
    46621 ttttgtatat aaaaatccca agtaaataca taaaaaataa aaaaattgaa acttttagcg
    46681 caccttgtac gtcatattaa gtgaggcaga aaggagtgga gaatgatttt tactggatat
    46741 aagaaaaaac ggcagatcga aaaataccgt cacctgctga atggactcat cgaaactgat
    46801 tatcaaaaaa tgttccgtat cacgatgaat tacgtacatg ataaagaaga ggccttagat
    46861 gtgatgcaag atagttttca taaagccctg aactattttg aaaaagaaaa agagatcgag
    46921 cattttagtg catggtttta tcggatattg atccgcacgg cattagatag ttggcgcaga
    46981 caaaaaagga cgcctgtgga cttatttgaa attcaagata tcccccaaca gttaccttta
    47041 gaaacttccg ttgcagagct ccatatgatt ttagataaaa tagacagtcc ggaaaaagag
    47101 attctgatcc tctacttttt tgaaggattt cgtttgaaag aaattgcgac tatcttggat
    47161 atgaatgaaa acacagttaa aacgaaaatg taccgctcat tgaatgcttt acgtcaaatc
    47221 ctagaatgaa ggagagaaga gtgccatgta ttcccaaaaa gaaaatttaa aaaaactaaa
    47281 gaaaacttat aaagaacaag caattcccaa agggcttaaa gaggagatcc gccagcgttt
    47341 tattatcgaa gagcaatcat ttataaaagc aaagcggaga aagagacgtc tccaagggtt
    47401 cggagtatct ttggctatcg taatgattac aacaagtagc cttcttttta atagtcaagt
    47461 tcgaagcttt gcagaagatt tacccatctt aggatcagtc attgaattga tcttaggcga
    47521 acgctttact gatcgatctg aaaaaatcga tattcaggta ccgaagatca atacacaaaa
    47581 tgagaaagaa aataaaacaa ttcatggatt aaatcaaaaa tactttcgcg aaggacaagc
    47641 agattttgaa aaagctgaaa aagaatatgg aaattttgaa acggatcatt accaagtttt
    47701 gggagattat cagaaaatcg tagacgataa ccggtttctc gtgattgaaa ggcagatcac
    47761 ccaaacagca gcagatagcc atgttgaaaa aagatacgac acaatcgaca agaaaaacag
    47821 tgtccagctc tcacttccac ttttattcaa agatgacacc tatctatctg ttttgacaaa
    47881 agaagtcaaa cggcagatgg ccgaacaagt caaggaagat ccatctaagt actattggac
    47941 tgagcaagat attcaagaag ggacgataga aaaaccaaca cttgttacgc ctacacgaag
    48001 tttttatttg aacaaagagc atcaattagt tttgactttt tctcaatacg aaattgctcc
    48061 tggttatatg ggtacaccag aatttgtcat tcctaagtcc gtgacaaaaa cgattttggc
    48121 atcggaagat tatttggatc attagacgag aagtagttgc gactagcagt ttcaattttc
    48181 aagtaagtac aagtaaataa taagaagaga aaaagagaaa gaagagatat agtgaaaaga
    48241 aattatttag ttatcagtat tattgcttta tccgtgcttt tgtttttcga aattacttcg
    48301 gtggcctcct tgatcaccga agtaaaagga caaacacact cttcggagca ttcgtcacaa
    48361 tctttcaaac aatcagaaca atcaaccatt aaaagccaat caactgaaac agctaacgtc
    48421 aaagaagcca ttcaagaatc agcggaaaag gtacaaagtc agtcatcaca atcaacacaa
    48481 gacagtacaa aaacgagccg agatccggct gtcaaaagcg tcgcaatcag ctttgatgat
    48541 ggaccaggag cgaccacaac accgcagctt ctgcgaatct taaaagaaaa aaacgtgcat
    48601 gccacttttt tcgtcttagg agaaaatacg gcgcagcatc ctgaaatcgt gaagcagaca
    48661 gctgaagcag ggcacgagat tggcaatcac acatacgatc atcagaatct ggccactctt
    48721 tctgcccagt cgatgacgga ggaagtgacg aaagcagaca ctgagatcaa aaaagtcact
    48781 ggcaaaaccc ccacttttgt tcgtccgccc tatggttctg tcacgaatgt aggagcaacg
    48841 atcatccagc gaccgatcat cgaatggtct gttgattcag aagattggaa gacaagaaat
    48901 cctgatttga ttttgcagaa gatccaagca acagtatacg atggggcaat tattttattc
    48961 catgatatct atcctgaaac gattcgtgcc gttcctcaag tgatcgatta tctgaaagag
    49021 caagggtatc gcatcacgac agttggggat ttattagggc atccaacggc tgtcgagaac
    49081 tattatggca gaaacgatca tcggccagtt cagtaagtga gacaagtatc tttaataaat
    49141 aacacatttt aataagtagt tgaacacata actaccttac ttgcaaaggc ttaacaatat
    49201 ctatacaggt acaacaaaaa actgtccacc aattttcttt ggcggacagt tttcacttaa
    49261 aaataaagaa gcaactattc gtaaattatg ctaaaatagc agtagaacgc gtcctgaggg
    49321 aatcgaaccc ccgtctcaag aaccggaatc ttacgtgata tccactacac taaggacgca
    49381 agcactggga taattgtaac taaacagata aaaaattaca aggggattgg acaaaaaaat
    49441 gaaatttgag caaaattttt cacaaaaaca gcagcagaca cagaaactag ccatgacgca
    49501 acagttgcaa cagtccatcc aagttttaca atataacagt gaggagctgt tggcattcgt
    49561 tgagaatcaa gcaatggaaa atccattggt ggaagttgtt gagccggaat ggcagccaga
    49621 ttacataaaa gcttcttcat cttcttatga aggagaagag acaaattacc taaatcagat
    49681 tcctgataca aaagggtctt tatttgattc attgatcgaa caagtccatt tgaattaccg
    49741 agatactttt ttgaggaaaa tcgttcttta tcttgtagaa tacatcgatt tgaacggatt
    49801 tttgacgatt agtttagaag aagccggaaa agagattgga gctacgccta tccaaatgct
    49861 cgatgcgttg actctgattc agcaattaga accagctgga gtaggtgcac gtagtttgca
    49921 agaatgtctc atgcttcaaa cagaacgaga tgattatgcg ccggaattag cttatatcgt
    49981 gttggaagaa tgttttgagg aactggtgga gcgaaaatgg aaagaaatcg cacaacgttt
    50041 ttctgttgat ctacatgctg ttcagcagat attcgactat ttacaaacgt tgagtccaag
    50101 ccctggccgg attttcgacc gtagcagcga attgtacatt attcctgatg tccgggtatt
    50161 agtggacgac gataaaaatg tacaagttat ctcaaaccga aaaaatcagc caaatatacg
    50221 ctttcaagag agttacttca aacaaatgtc acaacaagca gataaagaaa ctgaaaatta
    50281 tttaaaagaa cggaaacagg aattcgaatg gttgaagaaa acgatcctac aacgtgggga
    50341 tacaattttg cgggttgctc aagtcattgt ttcccggcaa aaagctttct ttatcgacaa
    50401 ggaacgtcca atcaagcctc ttacgttaaa ggaagttgca actgagatag atgtgcatga
    50461 atccacggtc agtcgtgcag tcaacgggaa atatttggaa acagacttcg gtgtatttga
    50521 attaaaaaaa tttttcacta cacgaattcc tacgaatagt acagaacaga cagaagacct
    50581 ttcagcagat acagcgaaga aaaagctgca ggaattagtc gatcaagaag acaaaaacaa
    50641 gccgttgtct gaccagaaat tggttgaatt gctgaaaaaa gatgagatag cgatctcaag
    50701 acgaacagta gcaaaatatc gtgatttatt aggaatcccg agttcatcaa aaagaaaacg
    50761 ttatgataac taatcagtca aaagactaga aaaagtattt cactttttct agtcttttta
    50821 gttgaaaaac gagagcaaaa ctgttagact tacagtcgtg aggttgagat ttccctttat
    50881 tgatgtgaaa ttcgaatagt taatttcttt ttttatcgta tatgggtcgt tttaagtcta
    50941 cattggacgt aaagtgtcca actaaggaga tcgctatgct ggatgaaata aaaatgattg
    51001 aatctgttgc accagatatt atcgaagtgt tgcaagaacg ctataaaatt ttacgaaata
    51061 tctattggat gcagccgatt ggacgtcgaa gtttatcgga aagtatgggg atcacagaac
    51121 gagtattgcg aacagaaaca gatcttttga aaaatctgaa tttgatcgac acctcaaaaa
    51181 gcggtatgac gctgacaact aagggcgaag aagtttatca aagccttgaa aacttcatgg
    51241 atcagctttt aggtacgcat caaaccgaac aacagttggc agaatatttt ggtaaccaac
    51301 gctgtatcgt tgtttcagga aacagcgaag agcagaccaa agtggctgat gcattcggcg
    51361 aagcgctgag cgaagcccta gaccgactac ttccagaagg cgagaatatc atcgctgtga
    51421 tgggaggaac tactatggct gtggtagccg aacaattgtc taatctggaa aataagaaac
    51481 ggcataacct gtttgtccct gcaagaggcg ggattggtga agcgataact gtacaggcaa
    51541 attctgtcag tgcaagaatg gctgctaaag caaacggtaa ccaccgtgca ctttatgttc
    51601 cggaacaatt aagtctggca acttacaatt ctttactcaa tgagccatct atccaagaag
    51661 tcttaaactt gattagtgaa gcgaattgtg ttattcatag tattgggcgt gcattacaca
    51721 tggcagcacg tcgtaagatg actgaaaaag aattagtcat gctgaaacaa gcaaatgctg
    51781 ttgcggaatc ttttggctac tttttcaatg aaaaaggtga agtcgtttat aaggtcccta
    51841 gaatcggcat agaattgcaa gatttggaaa aagtcccgat catcatggca atcgcgggcg
    51901 gaaaatcaaa agctaaagca attcgagctt atatgaaaaa tgcaccaaaa caaacgtggc
    51961 tcatcactga tgaagctgct gcaaatgaga ttttaaaagg ggtaaccctt taaaataaat
    52021 atttttttga ttttcataag gaggaaatct taaatgacag ttaaagtagg tattaatggt
    52081 tttggacgta ttggacgtct agcattccgt cgtatccaag atgtagatgg aatcgaagta
    52141 gtagcaatca acgacttgac agatgctaaa atgttggcac acttgttaaa atacgacaca
    52201 actcaaggac gtttcaacgg aacagttgaa gttcatgaag gttcattcaa cgtaaacgga
    52261 aaagaagtta aagtattagc taaccgcaac ccagaagaat taccatgggg cgaactaggc
    52321 gtagatatcg ttctagaatg tactggtttc ttcacttcta aatcagcagc tgaaaaacat
    52381 ttaacagctg gtgctaaacg cgttgttatc tctgctcctg gcggaaacga tgttccaaca
    52441 atcgtttata acacaaacca cgaaacatta actggtaaag aaacagttat ctcaggtgct
    52501 tcatgtacta ctaactgttt agctcctatg gctaaaacat tgaacgacaa atttggtgtt
    52561 gttgaaggat taatgacaac tatccacgct tacacaggtg accaaatgac tctagacgga
    52621 cctcatccta aaggtgactt ccgccgtgca cgcgctgctg ctgcaaacat cgttcctaac
    52681 tcaacaggtg ctgctaaagc tatcggttta gtaattcctg aattgaacgg taaattagac
    52741 ggagctgctc aacgtgttcc tgtaccaaca ggttcattaa cagaattagt aacagttctt
    52801 gaaaaagaag taactgttga cgaaatcaat gcagcaatga aagaagcttc aaacgaatct
    52861 tacggataca acacagacga aatcgtttct tcagatatcg ttggtatgac ttacggttca
    52921 ttatttgatg ctacacaaac taaagtaatg acagttggcg acaaacaatt agttaaaact
    52981 gttgcttggt acgacaacga aatgtcatac actgcacaat tagtacgtac tttagaatac
    53041 ttcgctaact tataagatag acaaacgtac aagtgtaaat tatagacatg tacatttaaa
    53101 agcggggaag caatcgcgct tcttccgctt tttttgataa taagtttatt caggaggaaa
    53161 taaaatggct aagaaaacag tgaaagatat cgatttaaaa gacaaaaaag ttcttgtccg
    53221 tgttgacttc aacgttcctt tgaaagacgg cgtgatcact gacgacactc gtatcaaagc
    53281 agcattgcca acaatcaact acgttctaga acaaggcgga aaagcaatcc ttttctctca
    53341 ccttggacgt gtaaaaacag aagaagataa agaaggaaaa tctttagctc cagttgcaaa
    53401 acgtctaggc gaattattag gtaaagaagt aacattcgta cctgaaactc gcggagaaca
    53461 attagaagaa gcaatccgca acatgaatga cggcgacgtt gtcgtattcg aaaatacacg
    53521 atttgaagat attgacggta aaaaagaaag caaaaatgat acagaactag gtaaatactg
    53581 ggcttcatta ggcgatgtat ttgttaacga tgctttcggt acagctcacc gtgcacatgc
    53641 ttctaacgta ggtatcgctt caactggtat cccaacagtt gctggattct tgatggaaaa
    53701 agaaatcaag ttcatcggtg aagcagtaga agaaccaaaa cgaccaatga ttgcaatcct
    53761 tggtggcgca aaagtttctg ataaaatcgg cgtaatcgaa aacttgattc ctaaagcaga
    53821 taaaatcttg atcggtggcg gaatgactta cacattctac gaagcaaaag gaatcaagat
    53881 cggtaactct ctagtagaag ctgataaagt ggaactagca aaagaattaa tcgaaaaagc
    53941 aggcgacaaa cttgtcttgc caatcgacaa tgtctgtgca ccagaatttt caaatgatgt
    54001 tgaaacacaa gtaatcgaag gagacattcc tgatggtttg atggctttag acatcggccc
    54061 agcatcagta aaattatttg ctgatacatt acaaggtgca aaaacagttg tttggaacgg
    54121 acctatgggt gtatttgaaa tgagtaactt tgctaaaggt acaatcggtg tttgtgaagc
    54181 aatcgctaac ttagaaggcg caacaacaat catcggtggc ggagactcag ctgcagcagc
    54241 agaacaatta ggatttgctg acaaattcac acacatctca acaggtggtg gcgcaagctt
    54301 ggaattgctt gaaggtaaag aattaccagg actagcagca atcaacgata aataaacgac
    54361 caagtttgct caattctgac gagtaatcga tcaaaaggtg aagggagttt tcatgactcc
    54421 ctagccattt ttcaataaat taaggagtgt gttcctcaat gcgtaaacct attatcgcag
    54481 gtaactggaa aatgaacaaa actgcatcag aagcaaaaga atttgctgaa gcagtaaaaa
    54541 acaaaatccc ttcaaatgac aaagttgatt cagtcatcgg atctcctgca ttattcttac
    54601 aagaattaat gacatcagct gaaggtacag aactaaaaat ctcagcacaa aactgctact
    54661 gggaaaactc aggtgcgttc actggtgaaa cttcaccagc agctcttgct gatctaggtg
    54721 ttcattatgt gatcatcgga cattcagaac gtcgtgaata tttccatgaa acagacgaag
    54781 acatcaataa aaaagcaaaa gctatctttg caaatggtat gacaccaatc ctatgctgcg
    54841 gcgaatcttt agaaacctat gaagcaggca aaactgctga atggatcgaa ggacaaatta
    54901 cagctggttt agctgatctt tcagctgaac aagtgtcaaa catggttatt gcttatgaac
    54961 caatctgggc aatcggtact ggtaaatcag ctgacgcaca aatcgctgac gaaatttgtg
    55021 gagttgtacg tcaaacagtt gaaaaacttt atggaaaaga agtatcagaa gcagttcgta
    55081 tccaatacgg cggttctgta aaaccagaaa atattgctga atacatggca aaagaaaatg
    55141 ttgacggtgc tttagtcggt ggcgcaagct tgcaagcaga ttcatttttg tcattgttag
    55201 acgctgtaaa ataattgttg aaaatttgcg aaaaatgcaa gaaatgtttg cactaatgag
    55261 caaaattaac taaaatcgta tatgagggaa atcccttagg aaaaaacaaa ctcaaaggag
    55321 agacaaaaca tgtcaattat tactgatgtt tacgctcgcg agatcttaga ctcacgtggt
    55381 aacccaacaa tcgaagtaga agtatacaca gaaagcggag cttttggccg cggaatggtt
    55441 ccttcaggtg cttcaactgg tgaatacgaa gcagttgaat tacgtgacgg agacaaagct
    55501 cgttatggcg gtaaaggtgt aactaaagct gtcgacaacg taaacaacat cattgctgaa
    55561 gcaatcattg gctacgatgt tcgtgaccaa atggctatcg acaaagctat gatcgcttta
    55621 gacggaactc ctaacaaagg taaattaggt gcgaacgcta ttttaggtgt ttctatcgct
    55681 gttgcacgtg cagctgctga ctacctagaa gtacctttat accactacct aggcggattc
    55741 aacacaaaag tattgccaac accaatgatg aacatcatca acggcggatc acatgctgat
    55801 aactcaatcg acttccaaga attcatgatc atgcctgttg gcgctccaac attcaaagaa
    55861 gctctacgta tgggtgctga agtattccac gcattagctt caatcttgaa agctcgcgga
    55921 ttagcaactt ctgttggtga cgaaggtgga tttgctccaa accttggttc aaacgaagaa
    55981 ggttttgaag taatcatcga agcaatcgaa aaagctggct atgtacctgg taaagacgtt
    56041 gttcttgcta tggatgctgc ttcttcagaa ttctacgaca aagaaaaagg tgtttacgta
    56101 ctagctgact caggcgaagg cgaaaaaaca actgacgaaa tgatcaaatt ctacgaagaa
    56161 ttagtttcta aatatccaat catctcaatc gaagatggat tagacgaaaa cgactgggac
    56221 ggattcaaga aattaactga cgtattaggc gacaaagttc aattagttgg tgacgacttg
    56281 ttcgtaacaa acactcaaaa attatctgaa ggtatcgaaa aaggaattgc taactcaatc
    56341 cttatcaaag tgaaccaaat cggtacatta acagaaactt tcgaagctat cgaaatggct
    56401 aaagaagctg gctacacagc agttgtatct caccgttctg gtgaaacaga agattcaaca
    56461 atctctgata tcgctgttgc aacaaatgct ggccaaatca aaactggttc tctatcacgt
    56521 actgaccgta ttgctaaata caaccaatta ctacgtatcg aagaccaact tggtgaagtt
    56581 gcagaataca aaggtttgaa atctttctac aacttaaaaa acaaataatt cttttctaat
    56641 gaagatacat gatcgactat tgaaagatag tcgagtatga tcacttagtt aagtaatcga
    56701 actctctact tgctgaaata tcagcaggta gagagt
//
LOCUS       DACEPD010000008        55716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.008, whole genome shotgun sequence.
ACCESSION   DACEPD010000008 DACEPD010000000
VERSION     DACEPD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.008"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(229..2121)
                     /locus_tag="IW797_000444"
     CDS             complement(229..2121)
                     /locus_tag="IW797_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1223243.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2118..3845)
                     /locus_tag="IW797_000445"
     CDS             complement(2118..3845)
                     /locus_tag="IW797_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1223244.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3861..4511)
                     /locus_tag="IW797_000446"
     CDS             complement(3861..4511)
                     /locus_tag="IW797_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1223245.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4715..5011
                     /locus_tag="IW797_000447"
                     /pseudo
     CDS             4715..5011
                     /locus_tag="IW797_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            5183..5803
                     /locus_tag="IW797_000448"
     CDS             5183..5803
                     /locus_tag="IW797_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ1223246.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5895..7061)
                     /locus_tag="IW797_000449"
     CDS             complement(5895..7061)
                     /locus_tag="IW797_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1223247.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7073..7426)
                     /locus_tag="IW797_000450"
     CDS             complement(7073..7426)
                     /locus_tag="IW797_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1223248.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7554..8576
                     /locus_tag="IW797_000451"
     CDS             7554..8576
                     /locus_tag="IW797_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ1223249.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9015..9449)
                     /locus_tag="IW797_000452"
     CDS             complement(9015..9449)
                     /locus_tag="IW797_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223250.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9559..11418)
                     /locus_tag="IW797_000453"
     CDS             complement(9559..11418)
                     /locus_tag="IW797_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1223251.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11536..12696)
                     /locus_tag="IW797_000454"
     CDS             complement(11536..12696)
                     /locus_tag="IW797_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ1223252.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13003..14427)
                     /gene="araA"
                     /locus_tag="IW797_000455"
     CDS             complement(13003..14427)
                     /gene="araA"
                     /locus_tag="IW797_000455"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ1223253.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14443..15141)
                     /locus_tag="IW797_000456"
     CDS             complement(14443..15141)
                     /locus_tag="IW797_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1223254.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15098..16759)
                     /locus_tag="IW797_000457"
     CDS             complement(15098..16759)
                     /locus_tag="IW797_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ1223255.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16977..18377
                     /locus_tag="IW797_000458"
     CDS             16977..18377
                     /locus_tag="IW797_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ1223256.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18629..19708)
                     /locus_tag="IW797_000459"
     CDS             complement(18629..19708)
                     /locus_tag="IW797_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1223257.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19794..20753)
                     /locus_tag="IW797_000460"
     CDS             complement(19794..20753)
                     /locus_tag="IW797_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ1223258.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLSYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20765..22288)
                     /locus_tag="IW797_000461"
     CDS             complement(20765..22288)
                     /locus_tag="IW797_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1223259.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22298..22861)
                     /locus_tag="IW797_000462"
     CDS             complement(22298..22861)
                     /locus_tag="IW797_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1223260.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22867..23730)
                     /locus_tag="IW797_000463"
     CDS             complement(22867..23730)
                     /locus_tag="IW797_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1223261.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23744..24640)
                     /locus_tag="IW797_000464"
     CDS             complement(23744..24640)
                     /locus_tag="IW797_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1223262.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24815..26086)
                     /locus_tag="IW797_000465"
     CDS             complement(24815..26086)
                     /locus_tag="IW797_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1223263.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26076..27593)
                     /locus_tag="IW797_000466"
     CDS             complement(26076..27593)
                     /locus_tag="IW797_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ1223264.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27791..28681
                     /locus_tag="IW797_000467"
     CDS             27791..28681
                     /locus_tag="IW797_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ1223265.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28754..29578)
                     /gene="nadE"
                     /locus_tag="IW797_000468"
     CDS             complement(28754..29578)
                     /gene="nadE"
                     /locus_tag="IW797_000468"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ1223266.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29590..31062)
                     /locus_tag="IW797_000469"
     CDS             complement(29590..31062)
                     /locus_tag="IW797_000469"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ1223267.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31699..32061)
                     /locus_tag="IW797_000470"
     CDS             complement(31699..32061)
                     /locus_tag="IW797_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1223268.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32284..33036
                     /locus_tag="IW797_000471"
     CDS             32284..33036
                     /locus_tag="IW797_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1223269.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33176..34099)
                     /locus_tag="IW797_000472"
     CDS             complement(33176..34099)
                     /locus_tag="IW797_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1223270.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34114..34386)
                     /locus_tag="IW797_000473"
     CDS             complement(34114..34386)
                     /locus_tag="IW797_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223271.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(34856..36481)
                     /gene="groL"
                     /locus_tag="IW797_000474"
     CDS             complement(34856..36481)
                     /gene="groL"
                     /locus_tag="IW797_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ1223272.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(36532..36816)
                     /locus_tag="IW797_000475"
     CDS             complement(36532..36816)
                     /locus_tag="IW797_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ1223273.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            37041..37703
                     /locus_tag="IW797_000476"
     CDS             37041..37703
                     /locus_tag="IW797_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1223274.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(37779..39071)
                     /locus_tag="IW797_000477"
     CDS             complement(37779..39071)
                     /locus_tag="IW797_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1223275.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            39241..39870
                     /locus_tag="IW797_000478"
     CDS             39241..39870
                     /locus_tag="IW797_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAQ1223276.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(39973..40782)
                     /locus_tag="IW797_000479"
     CDS             complement(39973..40782)
                     /locus_tag="IW797_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1223277.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(40837..41706)
                     /locus_tag="IW797_000480"
     CDS             complement(40837..41706)
                     /locus_tag="IW797_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223278.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESINAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(41707..43023)
                     /locus_tag="IW797_000481"
     CDS             complement(41707..43023)
                     /locus_tag="IW797_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223279.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(43020..44855)
                     /gene="walK"
                     /locus_tag="IW797_000482"
     CDS             complement(43020..44855)
                     /gene="walK"
                     /locus_tag="IW797_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ1223280.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(44860..45564)
                     /locus_tag="IW797_000483"
     CDS             complement(44860..45564)
                     /locus_tag="IW797_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1223281.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(45754..46614)
                     /locus_tag="IW797_000484"
     CDS             complement(45754..46614)
                     /locus_tag="IW797_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1223282.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(46601..47170)
                     /locus_tag="IW797_000485"
     CDS             complement(46601..47170)
                     /locus_tag="IW797_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ1223283.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            47551..47623
                     /locus_tag="IW797_000486"
     tRNA            47551..47623
                     /locus_tag="IW797_000486"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47584..47586,aa:Lys,seq:ctt)
     gene            complement(48119..48505)
                     /locus_tag="IW797_000487"
     CDS             complement(48119..48505)
                     /locus_tag="IW797_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1223284.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(48502..49023)
                     /locus_tag="IW797_000488"
     CDS             complement(48502..49023)
                     /locus_tag="IW797_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1223285.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(49025..50059)
                     /gene="sppA"
                     /locus_tag="IW797_000489"
     CDS             complement(49025..50059)
                     /gene="sppA"
                     /locus_tag="IW797_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ1223286.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(50092..51507)
                     /locus_tag="IW797_000490"
     CDS             complement(50092..51507)
                     /locus_tag="IW797_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAQ1223287.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(51476..53629)
                     /locus_tag="IW797_000491"
     CDS             complement(51476..53629)
                     /locus_tag="IW797_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAQ1223288.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            53887..54123
                     /locus_tag="IW797_000492"
     CDS             53887..54123
                     /locus_tag="IW797_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ1223289.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            54142..54357
                     /locus_tag="IW797_000493"
     CDS             54142..54357
                     /locus_tag="IW797_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ1223290.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(55060..>55716)
                     /locus_tag="IW797_000494"
     CDS             complement(55060..>55716)
                     /locus_tag="IW797_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1223291.1"
                     /translation="FKIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDLNHEVNGIELAE
                     VIRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLLLAQER
                     IDATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYGRISEL
                     EKKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKLKESTV
                     "
BASE COUNT        16462 a        11464 c         9377 g        18413 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttaagg aagaggttgt aaaatcagcg
       61 gtttgacctg agtattagag atacaaaacg gaaaaatagc ttctcatatt ttgccgtttt
      121 ttgagctaat accgaaggac agctgattga acaccgttta ttcggaaaaa gggactgtga
      181 cacgactttt gtcacagccc ctaaaaaaaa taataaattt gtactattct atgaagcttc
      241 ttcttcagaa aactgactgt tataaagatc tgcatagaat ccgtcttttt ccatcaatgt
      301 atcgtggttt cctgtttcta cgatcgaacc atgattcatc acaataatgt tgtctgcatc
      361 gcgtattgta gacaagcggt gagcaacaac gaaacttgtc cggttttcca ataggcggtt
      421 catcgctttt tgaatcagta tttctgttcg tgtatctaca ctagatgttg cttcatcaag
      481 gatcaggaca tctggatttg ctaagaatgc ccgcgcaatt gtgattagct gacgctgccc
      541 ttgcgaaata ttgcttgcat cctcgtttaa tacagtgtta tatccttctg gcaatttacg
      601 aacaaaatcg tctacgtgag cagctttagc agcctcaatg atttgttcgt ctgttgcatc
      661 gtcgttccca tatttgatat tgtcatagat acttcctgta aacagccaag tatcttgcag
      721 taccattgaa aactgtgcgc gcaattcatc acgtgaaaga tcacgggtat ctacaccatc
      781 gtaacgaata ctacctccag aaacatcgta gaaccgttca agaaggttga tcagtgtcgt
      841 cttccctgca ccagtcggac ctacaatagc gaccatctca ccaggtttga catccaagtt
      901 aaaatcagtc attagcaagt tatctggtgt gtagccgaat tgaacatttt caaaacgaac
      961 tttgtatggt gtatcttttt cttctggaag gccagatttt tcattggaca tctcttcttc
     1021 atccaacact tcaaataccc gttcggctga agcaactgtt gattggatag tgttcatcaa
     1081 acttgcaagt tgagtgattg gttgagagaa ctgtgttgta tattgcaaga acgcttgaac
     1141 atcccctaat gtcatgttcc catttgcaac ttttactccg cctagtacag cgacgaacac
     1201 atagcctaga tttttaacga aattcatcaa cggcatgatg attgccgaaa taaattgagc
     1261 tttccaacca gcggcataca gattctgatt ttctttttca aatacttcct ggtctttttc
     1321 agtatgattg aatgttttaa caatggtatg accaccatat gtttcttcta cttggttatt
     1381 caataaacct aaactttttt gctgatctgc gaaaaacact tgtgatttcg gcgctacgat
     1441 catcacgatg atcaagctta aagggatcat caataacgcg actaacgtca attgccagct
     1501 gattgaaagc atcatcacga tgaccccaat caatgtgact gcactagtta caaattgagt
     1561 caaattttgc tgcagcgtgc ttgcgatgtt atccatatca ttgattgctc gagacatgat
     1621 atccccattt gtatgtgtat cataatagga aatcggtaca cgattcattt tggcttccaa
     1681 atcatgacgt aattcataaa ctgttcgttg ggaaacacgt gtcataataa attgctgtaa
     1741 aaagttgaat acagcagaaa tcaaatacat cacgattact gtcgcaatga tctgcgcgat
     1801 tttatcaaag tctatcggaa attttgttac ttgttggcct tgttgcatct gttgagcgcc
     1861 agccattaca cctttgaata tttctgtggt tgctttcccc aagacctttg gtgtttggat
     1921 ttggaagatc acggctgcta ttgctagtac aaatacagcg atgatcgcaa ccatacgttt
     1981 agacatatag ccaaacaatc ggcgtaccgt tccccagaaa tttttaggct tttcaccttt
     2041 tacaccgaga tttctgccag gtccaccatg tcctggaccg cctgctggtc catgagaggg
     2101 acgtgtgttt gttttactca tgctaagtcc tcctctctta gttgtgaatg aacgatttct
     2161 tgataagttt cgtttgttgc taatagttct tcatgtgttc ctttgccaac cagttttcct
     2221 tcatctagca caaggatcat atctgcatcc attactgtac ttactcgttg agcaactaaa
     2281 acggtgatac tttctttcat attcgttttc aatgcttggc gaagattggc atccgttttg
     2341 aaatccaacg ctgagaagga atcatcaaac acatagatat ctgctgtttt gactaatgcc
     2401 cgtgcaattg ccagacgctg tctttgtcca ccagagaagt ttcctcctcc ttgttccaca
     2461 tggctatcta agccatcttc catctctgaa acaaaatctt tagcttgtgc gatttctaat
     2521 gctttccaaa tcatttcatc tgttgcatcc ggcttaccat actgcatatt gtctcggatc
     2581 gttccagaga acagaactgc tttttgagga acaaagccca ttaattctcg caaattatat
     2641 tgagtcatgt cactaatgtc cgttccattt atttggatcg aaccagattc gatgtcatat
     2701 aatcgaggga tcaaattgac taaggtcgtt ttacctgaac cagtcccccc tatgattgcg
     2761 acgatctcgc ccgacttggc ttggaaatca atatcttcca atgcaagttt ttctgctcca
     2821 tgatatcgat aattgacatg attgaaggcc aatgttgcgt tttttccagc aaaagaaact
     2881 gttttaggat tttcagggtc atggataccg attttttcat ccaacacttc attaatacgg
     2941 tcagctgaag cttgtgcacg aggcaccatg ataaaaatag cggaaagcat cataaagctg
     3001 atcaagatct gcatcgcata agtcataaat gcaatcagat taccgacttc caaagtcata
     3061 tctgctatgt agtgtccacc aaaccaagtg attgctacgt ttgtaccgct catgatcaat
     3121 gtcatcattg gcatcatcaa tgcgacgatc gtattaactt tgattgccgt atttgtataa
     3181 tctttattcg ctaaatcaaa acgattttct tcaaagcgtg tcttatcgaa tgctcgaatc
     3241 actctgaccc ctgtcaatcc ttctcgaaat accaggttta aacggtcagt tttcttttgc
     3301 atacttttga ataatggaac agcaaagtac atgattccgc cgatcaaaat aatcattact
     3361 gggatgacaa ctaagaagat ttttgtcaac tgatgatctt tttgataagc aagtatgctc
     3421 gcgccaatca acgtgattgg tgcattgatc atcatacgta aaaacatttg tgtaaccatt
     3481 tggatttgag tcacatcatt ggtcgttctt gtgatcaaag aggccgttcc atatttatca
     3541 aacgcatctt ttgtcagact ctctgatttt ttatagacat ccgtacgcaa ttgcttaccc
     3601 agtttctgtg attcacgagt agcaaaaaat gtatttgcga tagcagctaa gatactgatc
     3661 aaagaaaagc cgatcatcac aaaacctgta tgccaaatat aatcgacatc tccttgcgct
     3721 acccctttat caataatatt ggaggtaagt gtcggcaaat ataaatctgc cagtacttgg
     3781 atcaccatga aaactgcagc tgcaattgct gaagtcaagg agatccgttt tacgattttt
     3841 agcatctagt tcccttcttt ctatttatat actccgtttt tcagccaaga aagtttcaaa
     3901 ttgaattcac tgattgcttg ctctactgag taatcttcag atgttgaaaa taagcgatac
     3961 gaatgcgcaa ttgttgtaaa tacagtattc atcactaatt ggatcagtaa ttccaattca
     4021 tgatcatttt ttactttcag cttcgtccga tcgatcaatt caagcaactc ttttgcatct
     4081 tgtttgtgtt ctttatgtgg atcgtgaggc gttttttggc gactcgtatg aggagcgact
     4141 tttcttgagg cacggtaatc catgttcata aacaagttac gataaaaaga agcattctcg
     4201 ccttccaaaa tctcataaat cattttagaa aaataggatt cgaatccttt gaatagatca
     4261 ccatcggatt ctttcattag ttgtaacata tctttagagc tgtcacgtct cattgtctca
     4321 aagtaataaa aataaagatc ctctttatct tcaaaatact gatagaaact gcctctaggg
     4381 atatccgcta atttgacgat attagcaatc gaagcttctt ttaagggtac tctagaaaac
     4441 tcgattctcg cagcctcgat tagtcttttt tgtttttctt taggcaaatg gaaaaatgtc
     4501 tgtgtcggca tatggatcct cctttctttc aagatgacac attgtcatat gacgctgtgt
     4561 cattataaca cctcctacct gttttgcaag acatttgcat agtttttttc attatcatcc
     4621 gatacattaa aaaagtgata agaggaatcc ttccatttga attaatgaga gttgcccatt
     4681 aaaatagcca catactaaac aaaggatgag ataaatggcc caatcaatta gtgtccaaga
     4741 cttcaataaa ttgcctctcg atgctgacag catcgtttta gatacaagaa ataccttaga
     4801 ttacacaaaa aaccacttaa gccactcgct ttctatcccc gctgctatct tgccaagaca
     4861 attaaaaaat ctagatccaa agaaacttta atatattcta agctatacag gcaggcgatc
     4921 agaagcatta gccgatcaac ttactgctaa tggttttcgt gctgtttata tcattggtgg
     4981 gatggaagct cttcagtcga gtgttgcttg aatattgctt gttagattca aaagaggctg
     5041 tgacaaaaaa tcgtgtcaca gcctctttta taccctgtct ttttttagtt atttattctt
     5101 caaccttatc aatatgactt gatagccttt tctttttctc atagttataa caatcatagc
     5161 aataaaagga gaaggtaagc atatggcatc tgttttagta gtcaaaggtc atcccctaac
     5221 tgcagaagaa tcgcgcacag ttaaggcatt gacgtctttt ttaacaagtt ataaagaaaa
     5281 tcatccagat gatgaagtga ctgttttgga actttatcgc gatgatattc cggaaatcga
     5341 tgaagaatta ttatcaggtt gggaagcatt acgtgctggt gcagaattca cttccttatc
     5401 agaaagtcaa caacaaaaaa ttgctcgttt caatgaattg actgatcagt tccttgctgc
     5461 tgataaaatc gttatcgcaa acgccttatg gaatctaaat atcccaacaa aattaaaagc
     5521 ttggttcgat accgtcaatg tagctggaaa aacgttccgc tatacagaaa acgggccaga
     5581 aggattagtg acaggtaaaa aagcactgca catccaatct aatggcggtg tatacaacgg
     5641 acaagacttt gcctctcaat atgtaaaagg tattttaaac tttgtaggca tcgatcaagt
     5701 agatcagcta ttcatcgaag gaatcgatta tgaccctgac cgtgcagatg agttgatgca
     5761 aaatgcatta gataaagctg ctgctttagg taaatcattt taaaaattga gataaatgca
     5821 tacaaaagag acagaggaaa aaatcttcaa agcagcattt gtaccttgga tattttccca
     5881 tctgtctctt tttgttattc tttctgcttt ttcaacggat tttcttcata aagagaagag
     5941 attgttgtgt accattcaaa aacatgagta atcactactt tagctgctgc ataaacagga
     6001 atccccaaga tcacaccgat gattccgaac aatttcccag aagtcagcag aactaataaa
     6061 atcgttactg gatggattgc taactgattc cctagtacta atggagagat caagcgtcct
     6121 tctattgtct gctctactgc aaaaacgatc agtactttga tgatcatgaa tggacctgct
     6181 acaatggcta aaaagatagc cggaatcatc gctaaaaatg atcctaagta agggatcagg
     6241 ttcaaaaaac ccgcaataat ccccaacgtg acagcatact ctaaaccaat gaccgcaaaa
     6301 ccaatcataa acatgattgc cacagcaaaa gccacagtta gttgtcctct tatataagaa
     6361 gaaacttgac tattcatttc actcagcaca ttcaacgtcg gtttttgcat gcgtgtcggt
     6421 aaaaatttga caaaataagg tgctaattgt ttaccgtctt tcaatagata aaataagata
     6481 aacggcatcg tcagcagagc cactaaaata gtcgctaccg cgccgacaaa acttccaaga
     6541 ctctgaactg tactcttaga tatatcttgg atcatatcgc ctgccgaatt catgaactta
     6601 tcacctgctg tttccaactg ttcgcgaaat tggttgaaca gcgggtcccg caaaatctcg
     6661 gttaatttat tatcaatcgt atctacatat tgagggaagt tactgatgaa gctcactgtt
     6721 tgttcctgaa tcttagggat gatcactacg ctcccccatg cgattagtgc aacaaccaaa
     6781 acaaacagcc caatgatact gtaaagcctt ggtacttttc gcttttcaaa gtaatcaact
     6841 accggattca tcaagtaata caagattcct gctaaaatga taggcagacc tacgattgct
     6901 aaaaattgcc agattggtgc aaacaaatat gaaacttttg taaataaaaa gacgatcagc
     6961 aatattaaca atacaacaag taaggctgtg actacttgat tatttaaaaa ccagcgccaa
     7021 aaccacgata ccggtctgtt cttcttattt tttccttcca taattcctcc tgttagttat
     7081 aaaaaatttg gcttcccaaa ccaatagcag gcagtctatg cggatgaaga tagattccat
     7141 ttgcaatttg tccttcttct ggacaagcat caaaaatcaa tgatacgtga gctactgtct
     7201 ctaaattccc attggccatc ggaccgacat aatcgatcgt atatacctga tcatcaaaag
     7261 caattgttcc gcctgctttc aaagaaaaag gttcgtcttt tgatacttct tgaatcaccg
     7321 aatattttcg taaggcagct gttgctgttt tatcgaacaa aatcagcatg ggttcttcct
     7381 tgtttattgc ttctgtccca atttctgtaa tctttgcctg aatcattgca ctctctcctt
     7441 taattgcttt caaaaacagt ataacacatt ctcattcttt agccgtgtgg aataattatg
     7501 tgatagaata aaaacgaaaa ctatttcatt acaattagga ggaatcagct catatgatcg
     7561 aaaagataat tttaggaacc tatacgcgtc gcgaaagtaa aggaatctat acgatcgacc
     7621 ttgatacaga aaaagaagaa ttagccaacc tttcattagc tgttgaagaa aatagcccta
     7681 cctatgtagc aaaaagtaaa gctggcaatc tttacacagt tactagtgtg gatggattag
     7741 gtggtgccgg tgcgtacgat aaggattttc attttttgaa tgcggtaaca gagtctggcg
     7801 ctcctttgtg ttatgtagcg gttgatgaag cacgtcaatt actatacggg gcgaactatc
     7861 ataaaggtga agtaaatgtc taccatatat taggagatgg cgaactgaaa gcagcagatt
     7921 caatattcca tcaagaagcg acaggtcctc ataaaaacca agatcatgcg catgtccact
     7981 atacggatct gactcctgac caacgattag tcgtatgtga tcttggtaca gatcgtgtgt
     8041 atacttatga tgtgtccgaa aacggcaaat tgaatcttgt aactaccttc actgcagagc
     8101 caggaacagg ccctcgccat cttgtctttc atccaaatgg acaattcgct tatttatttg
     8161 gtgaattaga cagcacagtc cgcgtgcttt cttataataa agaaacaggt gcttttgaag
     8221 aattacagaa aatatcgacc ttgccagaag aattcgacgg tgaaaatgga ggggcagcta
     8281 tccgcatttc aaatgatggc aagttccttt atgcttctaa ccgaggacat aactcgattg
     8341 ctgtattcgc agtttttgaa gatggttccc acattcaaaa caagcaaatc atctcaacag
     8401 aaggggattt tccccgcgat ttcgcactag atccaagcaa taattttgtc gtgtgtgcca
     8461 atcagaacac cgataatctg acattatatc gccgtcatcc tgaaacagga ttactggaat
     8521 tactgaaaaa agatgtctat gtaccagaat gtgtctgtgt ttattttgat cgttagtaag
     8581 aaaaggagaa tgaaatgaat cagcaagacg tattagacag actacgggaa gaactaaata
     8641 ttcctttctt tgatgggaaa atcgaagata aagagtatag cgaagaggaa tatcagaaac
     8701 tcaaagccga tttacagaac tactttgaac aatatgtaca gaatgtagaa aactaatcag
     8761 ctttccatac tatctgaaaa gaacatcagg acaagacttt tccgtctatc ctgatgttct
     8821 tttctacaaa taaaaaaccg ccgagatggt tagactcgac ggaaaaggag tatttactcg
     8881 attttgagta agtacaaggt tgttgttggt tataggtatg tcattatcat aaaggatttt
     8941 gatcactcta tcataaaggt aatattacat ttatattaga taactctagt ttttattgtt
     9001 atcaaagatt tcttttattt tttcaacaac aaattagtca ttttttggat ggcagctgtc
     9061 aattggtctg tcgcttggtc ttgttttgct tcagatgtat tgaatagatc ttcatctacc
     9121 cctgattcaa gatcgatact ttggatcatt tcttcacagc ctgctacata tgaaagataa
     9181 gctttttcaa aatttttatg aatgcccatt gcttttgctg gtggacgcaa agtactgact
     9241 ttcttaagca ttgcccgata tttttttgtt ccttcttcaa ataacaggat gatctcttca
     9301 agagtcgatt tgtccagttc acttgttttt ccttcatcaa ttgcgtttct gatcacttca
     9361 tatttcggat gcattccttc tccgattgct tctgtttcct tgacgatttg atcgatggac
     9421 tgcacataat agccaatatt cggtaacatg ttcgttttcc tactttctcc attgttgtta
     9481 ttatttattc tacttgtttt tatcctcaaa tgacaagtac ttgaaaaaat ttaatggttt
     9541 ctatacagct cgccattctt attcgtgctc aaagaaacca tctgctaaac tatattgttt
     9601 ccctgcgaca atgtcatatt ggatcaattg atattcgtcg taaatctctt gttgcgagcg
     9661 attgaattgc gcttctttat accactgtcc gttttgatag tacagttctc gttcaaacgc
     9721 cggaagttcc tgttctaatg ataaaagcag ctgataaaag ccatttgttg tctgattcgt
     9781 ctgttccatt agattcgctg caaaataaaa cggactggtg attgcatcgt gagtcgtttg
     9841 cttttctaat tctcctttac tgtcgaacat aagaaattgt gtttcatgca atgtttgttt
     9901 gtcatttttt gcttggatcg tatctgaata gatccctgga agatgatccc cccaaaaaac
     9961 aactaaagtc cttctagaaa gattcttcaa ttcttccgtg aactgcttca gtgccgtgct
    10021 gctataggaa atatcttgca gataattctc taagtcaagt gtcccacttc catctgaagt
    10081 ttttgcagga tagtctagat tatcatactt tccatcgtag ggcatatgtg tctgcatcgt
    10141 gactaaatga atgaactgag gtgttttttc ttccttgaga agtgtcatta cttctttgta
    10201 agctgactca tcagaaatat atggattatt ctctatcgta tccgtatagg tcatcgtacg
    10261 ctcgctgatg aactggtcaa atcctaatgt ctgataaaca tcctctcttt tatacatcga
    10321 agtattgtat ggatggatcg ctgtcgtctg gtatgattgt gcgtttaacg ctgaaacgat
    10381 agaaggaagc tgatccattt ttggtacaag catcgtgtaa ggggtggtga gttgcgcatt
    10441 aaataacgcc atagaaaaac cagtcaatgc ttcaaattcg atatttgctg ttccaccgcc
    10501 atagttttga gaaagcatat tgccactata tgtttgatca gctacttcat aataatctgc
    10561 caatggctca ccagatactt ctacgccgtt taatcgacta ggatctgaga aactttcact
    10621 catcacgaac acgatgttcg gcgactcttc ctctacagtt ttattttttt catcggctag
    10681 cttctggtat ttttcagtga tttctttgat cttttctttc gaatatcctt caggttcatc
    10741 cattggttct accttcagat tgtacaaaaa cccaccgata aaaccagtgt tatagtagtt
    10801 cattttctgg ctgtaaggta tccatagtgc cgttttatta tatgcttttc gcaacaaatt
    10861 atcgggattg ttgaaattac tgatataccc tagcagtcca attgccacaa gcagtgtcgt
    10921 taatcggatc aattggaatc gccttccttt tttcaggctt cgaaacatgt accagcaaaa
    10981 caatcctatc acggttcctg cggcagctaa gatcactgta aacagcatag tgccggtcat
    11041 atctttcagc aatccgattt cagtgatcat cttcaaatcg tctggataaa tcggttcttg
    11101 ccgataactc attttcatat aatttgcaaa acctaataca ccgatggcta cgacatacgt
    11161 aagggaacct aaagggacag atcctgctag tgcaatcaga aaaataagaa taatcagcaa
    11221 gacgagacag gctaagaaga atttttccgt atgccaagaa aaagcaaatt tcaacgcaag
    11281 gtctacggat agatcatttt gacaccactg caaaaataaa ttactaaaaa caacaagaaa
    11341 aaagatactg atgattgctg ctgttccttt taaaatcttt ttctgtttgg tcgaaagtct
    11401 gcttatatat cttttcaaat gcttgctcct tcatttctat caataaaaga atgttgcttc
    11461 tcttatttta gttgtacgaa attcgaaagt aaagaacgga aagaagaatt aatgaaatcc
    11521 aaatattctc tttctttatg gcctttctgg tttctttaat aagcggacct ttcgtactcc
    11581 agaaattttt tctagctgtt gtttcacttg attgagctgt tcaaaatcag tactgtctat
    11641 gtcaatcaat atgtagacat gctcattctt atggttacgc gatatatctg cgatcgctag
    11701 ctcgttctca ttcagcagag aaaatatctc tgctaatata ttggttttct tttgataaaa
    11761 tatagtgaat cgaaacggcg cttggaaaaa cattcgtgcg gatggataat tgaccgcttc
    11821 tctaaccgta ccgaacaaaa gataatcttt taaggcttca actgccaaac gtccgctgtc
    11881 tgccaaagct tcctgagtcg atccccctag atgaggcaac ataagtatac ggtcattttc
    11941 taagaactct tcttctggaa aatcagtcaa gtatttagcc agatgttgtt tttccaatgc
    12001 ttgcataaca gcatatttat caacaatttc acttcttcct acattgataa gtacagcatc
    12061 ttttttcatt ttttctatat tcgcctgatc gatcaatcct tttgtatctt ctgtcaacgg
    12121 cagcatgact acgatgtaat ccgaggtaga aagaactgtt tctaaatctg cttgccgaac
    12181 ataatccaac tgggcatcgc ggatagaata acccaagaca tccatcccta agctatagca
    12241 acttagcgct acttttgtgc ctatcgctcc aagaccaaga agaccgatgg ttttgccttg
    12301 caattctcgt cccacataag cacttctttt gttttctgcc tgttctaaaa tattaggacc
    12361 tgttagtgtt tgtaccatac gacttgcttc gattatcgga cgggaagata agagcaaaca
    12421 gcacaaaaca agctctttta ctgcattagc atttacacct ggtgtattca tgacgatcgt
    12481 cccgttttca gaagcttttt caacgttgat cgtgttcacc ccaacaccag ctcttgaaat
    12541 tgctagcaaa tctggtgtaa gccattcatc tttcactttc gtactacgaa taactattcc
    12601 ttcggctggc aaatcatcta ttattttgaa atattcccct tgtaactttt ctatctcttg
    12661 ttctggaata gcatcaatca aatgaacgtg atacataaaa ccccctcctt actaacaagt
    12721 atctcgaaat ttctgcaatt agacaactgt ttaccatttg acttcctaga agcaaaaaga
    12781 gcagttagag cagagtaggc tgagtgataa aaataaggct atgacaagtt actgccataa
    12841 gccttatttt cgagagcaaa atattgaggg tgagatatct tgataaatgg tgttccgtta
    12901 gctgacattg ataataagac taaatttcaa aaaagttgaa aagcaaattt cgtcaatctc
    12961 ttcttatttc ttaaaactaa gtggcttttt cacaacctct ttttatctaa taaccactgt
    13021 ttccaactca actaattttg cccacgtttc aatttgatct gttgttagat ttagcgagac
    13081 aacggtatga tgaccgccac ctgcctgaat ccatgaatgg acaccttcat ggaaattcgg
    13141 tttagttttc cataacacac gtgcaacagg taaattaggt gcttcagtcg ttggttcaaa
    13201 ggcttctact tcattgatca gcagtttata atgcgtaccg aaatctgcca tcgatacgac
    13261 taccccttct cctgctttcc catcaaatac aagacgcgcc ggatcttctc tatctcccat
    13321 tgataaagga gaaacaacga ttcttggttt cgtcgcagca agcgtcggat ctacttccat
    13381 catatgggat tccaagattg cttcttggcc actagctagc tcgtaagtat aatcttccat
    13441 gaatcctgta tctttccctc gagccatgat tttcagcaac cgatcaatcg ctgcagtttt
    13501 ccagtcgcct tccccagcaa aaccgtatcc ttctgccatc aaacgttgta cagctagtcc
    13561 aggtagttgt ttcattccat acaggtcttc aaaattagtc gtaaaagcgt tatagcctcc
    13621 ttcttctaag aaacggcgaa taccgatttc ctgctgagct tgtactttta catgttcctc
    13681 ccatgtcttt tcttcataat caccatagtc gaaggtatac aattccttgt actcttcaaa
    13741 taattgatcg acttcttcgt ctgatacgcg atcgattact tgtacaagat ctccgatacc
    13801 aaaataatca acagtccaac caaattgaat ctgtgcttcc actttgtctc cttctgtgac
    13861 acccacgttt ctcatgttgt caccaaaacg agccactttt attccaaaac tttcattata
    13921 ggctactgca acatccatcc aatcagcaat ctctttttgg atctcaggtc gtttccagta
    13981 gccaacgacg attttatttt gtttatttaa tcgagcattg atgaacccat attcgcggtc
    14041 accatgagcg gattgattta aattcatgaa atccatatcg atcgttttcc aaggaatgct
    14101 ttcgttatat tgcgtagcta aatgaagcaa tggtttttgt agaagctttg tcccgcggat
    14161 ccacattttt gctggtgaga aagtatgcat ccaagtgatc acacctgcta cttcttctcg
    14221 ataattgacc tctttcatga tttttgtgat cgtatcagca gtaactgcta attcttgaag
    14281 gcggattgga taaggcaatt ggccattttc attcagttca tcgaccattt cttgtgcatg
    14341 tttctttact tctcgcagtg cttcttcccc atataaatgc tgtgatccta caacaaacca
    14401 aaattctttt tctccaatat tcaacataat tagccactcc ttttatttgt tttgtccgta
    14461 atacgcattt tttccgtgtt ttcgtaaata atgtttatct aaaatccgtt gaggcagctc
    14521 ttctgaaaat gaattgagct gtctggtaaa taaattcatc ttgcatactt catctagtac
    14581 gactgcgttc attaccgcac tttgcgcatc tttcccccaa gtaaatggac cgtgtccgtg
    14641 taaaagtacc cctggaattg ctaagggatc aattttgcgt tctttgaatg tttctatgat
    14701 aacttttcct gtttcatatt cgtacccgct gtcgatttcc tgttgtgtca aaaatcgagc
    14761 acaaggtacc gatccataaa aagtatctgc atgcgtcgtc cccatcgcag gaacatccaa
    14821 tcctgcttgt gcccagatcg tcgcccatgt cgagtgagta tggacgattc ctccgatttg
    14881 aggaaatgaa cgatataaaa cagcatgagt aggcgtatcc gaagagggat tgagtttacc
    14941 ttcaatgata tgattgtcca agtccacaac gaccatgtcg tctgctgtca tctcttcata
    15001 actgactccg ctaggtttga taacgaaata tcgtgtttca ggatcaaaag cgctcacatt
    15061 tccccatgta tatttgacga gtccgtgttt aggcaagtca agatttgctt ggtatacttc
    15121 ttctttcagt tgttccagca ttttctatca ctcctttttc ttgcaattga tgaatcgctg
    15181 ttttttcaat gtcgatcccc tcacggtaac gtctcataaa tatctgatag cctgctattt
    15241 cttctgctga aggtgtgatg gtgattcctt ggtcttgtcc aaacacgtct tctgccaaga
    15301 agttttctaa agacttccca tgtcgatcca tcatataggc tgctaataac gcgattcccc
    15361 acgctccacc ttctccggct gtgtccatca ctgtaactgg tgcctccata gcagatgcta
    15421 gaatccgctg tccaacttct ggtgttttaa aaatcccccc atgccccact aaacgatcaa
    15481 tttggacatt ctcagatttc aatatatcca ttccgattct cattgcacca aaagcactgg
    15541 ctaaatggat ccgcatgaaa ttcgctaaat caaaacggct acctggtgta cgtacaaata
    15601 atggccttcc ttcatttact cctgtaatgt tttcaccgga aaaataacca taagaaagta
    15661 atcccccgca atccgtatcg ccttctagcg ctttgttgaa taataccgtg aataatttat
    15721 ctctgcttat ctccataccc aaagaattgg taaattcttc aaaaatactg acccaagcat
    15781 tgatatccga agagcagtta ttcgtatgaa ccatgcctac tagttctcct gaaggtgtag
    15841 tgaccatgtc gatttccgga tggacatttt ctagttcttt ttctaatacg atcatagcga
    15901 aggcggaagt accagccgaa atgttccctg tccgtttggc tacactgttc gtagcgacca
    15961 taccggttcc tgcatcccct tctggcggac aaaccgggat tcctggtctc aaatttcccg
    16021 tagggtcgat cagataagcc cctgattcgc ttaacttgcc agcttcttct cctgctacta
    16081 gaactttagg caaaatgtga tccaagtcaa tcgctacgtg cgctttttcg attaactgac
    16141 caaaaatatt tttcatttct tgatggtagg ttttcgttga aggatcgata ggaaacatgc
    16201 ctgatgcatc tcctacgcct agtactttct caccagtaag ctgccaatgg acataacctg
    16261 caagcgttgt caaaaaatta atttctttca catgttcttc tttattcaaa atagattgat
    16321 acaaatgagc gatactccat ctctgcggaa tattgtattg aaatttttcc gttaatattc
    16381 gttctgcttc ttcagtgatt gtatttcgcc aagtacgaaa tggtactagt aactgttcct
    16441 tgctatcaaa tgctagataa ccgtgcatca ttgcactgaa cccaatcgac ccgattgtcg
    16501 taagattctc gccatagatt tcgaatactt ctgctgccat ttttcggtaa ctgacgcgca
    16561 gtcctttcca aatatcttct aatgaatagg tccagatacc attttctagt ttgttttccc
    16621 actcataact accagcggca ataggagtaa aagatgaatc aatcaataca gctttgatcc
    16681 tagttgatcc aaactcaatc cctaaagatg tacgaccatc tatgacatct aacatacttt
    16741 gcgcatttac ttccttcata atgatttaac tccctccttc tcgtcaaacc tgatcaaacc
    16801 gtctttttag tagcgcttac aatgattatt gtatactttt gtacgtacat gtcaagcggt
    16861 tatttacaag aatacaataa aaaacacttg ttttgtacgt acataagtta taaaatgaaa
    16921 ttgcttacaa aatctgatca accgccttct cgaaattcta gaaggagaga atacttatga
    16981 aaaaagaaaa aaaagtttcc tcatcgttta tttatttttt tggcgcattt ggcggcattc
    17041 tatttggata tgatattggt gttatgaccg gcgccttacc ttttttacag catgattggg
    17101 gtttggcagg taaagcttct ttgataggat ggatcacgtc ttcggtcatg ttaggtgcga
    17161 tccttggcgg ctctttatct ggtcttcttt ctgataagct tggacgacga aaaatgattc
    17221 ttctttctgc acttattttt atggctggtt ctgtcctttc tgcatctgcc cctcacaatg
    17281 gttcttattt tctgattgca gcaaggattc ttctaggttt agctgttggc gctgcatctg
    17341 ccctagttcc tgcttatatg tctgaaatgg ctcctgctag actaagggga agattgtcag
    17401 ggatcaatca agtgatgatt gcttctggca tgctcctttc ctatgttgca gattatttgc
    17461 taaaagtgtt gcctgaaacg atggcttggc gcgtgatgtt agggctcgct gctgtccccg
    17521 cgctgatttt atttttcggt gttttagctc taccagagtc accgcgtttt cttatgcaat
    17581 ctggccgctt ggaagaggca aaaagagtat tgaactatat tcggacacca aaagaagctg
    17641 aacaggaatt cgaacaaatc cagctgaatg tcaaacaaga aaagacaacc ggaacttctt
    17701 ggcatacttt gtttttagaa aaatatcgtt ccttagtcat tgccggtatt ggtgtagcag
    17761 tattccagca atttcaagga gctaatgcta ttttttacta tattccgctg atcgtagaaa
    17821 aagcgactgg gcatgcagcg agcgatgcat tgatgtggcc gatcatccaa ggaatcattt
    17881 tagttgctgg ttctttgctc tttttggtta ttgcagacaa gttcaatcgg cgaactttat
    17941 tgaaaatagg cggtagcgtg atgggacttt cattcatcct accagctgtt ttaggaactg
    18001 tactggatgc tcatacaaat tcgttgttga ttctcttgtt tttatgtatt tatgtcgcat
    18061 tttattcttg cacatgggct ccactgacat gggtaatcat aggcgaaata ttcccactag
    18121 ctgtccgcgg gcgagcatct ggcctagcct cttcttttaa ttggattggt tctttcctag
    18181 ttggcttgct ttttcctgtg atgactgctt ctatgtctca agaaatcgtt ttcggcattt
    18241 ttgggatcat ctgcttttta ggggttttat ttattcaaga gatcgtacca gaaactcgtg
    18301 ggaaatcttt agaggaaatc gaacaaagtg catctaaaaa aacttatcct aaacgtattt
    18361 ctatccatca ttcttaaaga ggttgtgaca gaagtggtca gcttcaagaa ataagaagga
    18421 accgcttagt ttccagtcac aaggaacaat ttcaaaaaac agcttctcat gtttgatgaa
    18481 tattgtgact tgtgaccagt tggtaggaga acaccgttta gtggagaggt ggtgaaagaa
    18541 acgttcagcc ttgagcgatg acaaaagctc tcacaaacat tgcgacgagt aaccgtagga
    18601 gcaataagag gcggtaaaaa cccgtctctt atttttcgtt ttctttcact gtttcccgtt
    18661 caactagtac aggttcatag agaatatttg gctgtgcctg tcctttttca atcgttgtga
    18721 cgatccaccg tgcggcatct tgtcccaact tttcttttgg atgcgtcaat gttgtcaatt
    18781 ttttattccc taaatggctt aaagaagaat cgtctgttcc tacgatcgat aggtcatctg
    18841 gaatatgata gccattttct gtcaacttat ctagcattaa actagctatt tgatcgttat
    18901 agcaaatgat gccatctaaa gcttgttctt ctttcaaccg ttgtactgtt ttttcagcga
    18961 ttgtttctcg tgtttccgtg gtgaaagtaa tgatatcttc cgtcaaaaaa cgcacattat
    19021 actgttcaca cgcgctgata aaacctttca tcctgtattt tccttgaaga tcgtcgattt
    19081 ttgtgatgaa caacagacgc tgatgtcctt tttttatcaa atattctgct gccataaaac
    19141 cgactttcac atcatctaaa caaatataag aggcatttaa ctcttcataa gcagcattga
    19201 tcatcaccat cggaatgcct tgttctctca aagcaacata aagtgctaag tttgggttat
    19261 actgattact tttcgtaggc tcaacgatca aaccatctac cccataagca atcattttct
    19321 tcaaacactc tttctccaat tcgtgatcgt tattcgtact ggctaaaagc aatgaatacc
    19381 cagattctcg taattcattt tcgattcctc ggataatcga cggaaaaata taatcagaga
    19441 gatatgtggt gatgacaccg atcgtttttg ttctgttcaa tggtgttttt tctttcttat
    19501 agctgtcatc tacatatgtg cccgaccctt tttctttacg cagatagccc tcatttacca
    19561 acacagcgat tgcttgccga atcgtatgtc tgctgacgtc atatatttct tgcagcttga
    19621 attctgaggg aatggttgta tttacttcgt attcctcacg aaggatcttt tgttttatct
    19681 cttctgcaat atcttgatat tttgccattt gccttgcccc tttttatttg tacgtacata
    19741 tatcataacg agtctgcaag gtttattcaa gcaaaaatca aggtttcttg tgctcataag
    19801 gcatacggaa caggttcacc aaattctgga aatccttgct cattccacgt aaagcattgc
    19861 gcgttcgcgt gccgattagg attgtctaac ggatcccctt tttggttctt ctctggtcga
    19921 gcatgataga tcaaaatatc aaagcacccg tcttctgaaa ccgtaaacga gttgtgacca
    19981 ggaccaaatt gcttattttt ttcagaagaa acgaatactg gctccgataa tttatgccat
    20041 gattctttta ataaaagatt cttgtgctca tcagcccaca gcaggcccat ggcatagttt
    20101 tcatctgttg cactaccaga atacgtaatg aatattttcc cattgcgagt aaggactgct
    20161 ggaccttcat ttactgaaaa accccgtttt tcccattcat actcaggtat agaaaggagg
    20221 atctgcttcc cctttaacgt ccaaggatta tccatttcgg atagataaag attagaattc
    20281 ccagtaattt caggatcttg ttgggcccac acataagata gctttccttg agaagagaaa
    20341 gttgttccgt ctaaactgaa agtttccctt ttcgttttta tttgtccctt ttctatccaa
    20401 tctgtattca ttggatctat atgctgattt tcaagtacga acatgcggtg atgatgagat
    20461 tcatcacgta tagttttatg attgcttgca gcaaagtaaa tataccattt tccattgata
    20521 taatggactt caggtgccca gatcaattca ctcatcggtc cttcttcatg agcctgccat
    20581 acaatcaggc ttgcagcatg ttctaattca ttcagtgact ttgcccttct taattcaatc
    20641 acttggtagc ctggaaccga tcctgtaaaa taataataac catctgtatg tttatatacc
    20701 caaggatctg cccgttgccg aacgatagga ttattataaa gtgtcttcat cataacaaat
    20761 ttcctcacat tttctttatt ctaattacat tccaagagta tttttctatc cctacattta
    20821 attcgtcatg tttttctatt gaaaaatagt ttatttcagg ttttattttt tctggttctt
    20881 cacgtgtatt tttgtccaac agatcgctac tggttaatgt gtagtgctct ccttgatcta
    20941 gcaaattgaa atcatgttca atcgttatct tttgttgttc atttgtgcga ttgatcaaga
    21001 aaatagtcag ctgattgccg ttatctacga tcacactgtc cacataagga acatttttta
    21061 tcttttcaca atcataaaca gggcaaatga tttgttgttt caatacttga cctttagcat
    21121 atttcgataa gtgttgcatc acataataag ttggattcct ccatgcttgt cctttagcat
    21181 ctgtcagtat taaaggtatt gtgtttacta acaatgattg acaactaatc tttacgcgat
    21241 ctgcgtgtcg taaaatagcc agccctaatg aaccaaataa taatgtatct tccatagtaa
    21301 agtaacacca atcgatggga ctaccgactt caaaatccct atgtcttttt tccggttggg
    21361 catgtacatt ccattcatca aacgctagat acattgtttt ctcacttctc aaatatcctt
    21421 ttacataatc acaagtagca acgatttctt caatttgacg atcgaatcga taagcttttg
    21481 caaggtatgt cgctgtatca tcacgctcat ttttaggagg tttagtcaag tcttcttcat
    21541 catacttatc aatatagtta tgcaaagcta aataatctac attctcataa gcttcttcta
    21601 atgcgatacg atcccattca gggtacgatg gtaaacgtgg cgtagagctt ccaactaaga
    21661 ttgtttcaat tgaatcatcc actaacttca tgacttttga ggcttcattt gctaacctac
    21721 catattcata agcactctta gcccccactt gccatggacc gtctagctca ttccctaaac
    21781 accataattt aacgccaaaa ggcttttttt gcccattttt tattcttaaa tcactccaga
    21841 aggttccttt tggaaaatta caatattcta ctaaattttt tgcttcatca attccttttg
    21901 ttcctaaatt caccgtgaat atcggtttcg cttgtacatg actgatccat ttaaaaaatt
    21961 cgtggagtcc aaaagaattt ggttctatac ctcgccatgc taaatcaatt ttttttggtc
    22021 gttgttcttt cctacctaca gtatccaccc aatcataacc agatgtaaag tttcctccag
    22081 gatagcgaat gacacctaat tctaattcct ttacataaga gaggacatct tgtctaaacc
    22141 cgtcaccatc agctgtttca tggcccggtt cataaatccc tgtatagacg acacttccca
    22201 tatgctcaac aaatgagcca aacagccgag aactgatctc tcctaccgta aaatcctctc
    22261 tgatttttac tttcacttct tcgtccaaca gctttcccta cctttcattt cctatagtca
    22321 agataataga tacaagtttc catacgccac cagcaaagat aaaaataaaa ggagggaaaa
    22381 gaaatatgag cagtacaaat cctaataaca atacgcaaca aagaatattt ctcaaaaaat
    22441 caaaaaatac tttccatgca ctttgttcaa aaatttgtaa aaatgtgata tctttgtttt
    22501 cagaaatcac atatatccag gataaaagaa acatggaata aataagtaaa actacatatc
    22561 cactaattga aagtacgatt gtctgttctt ctaataattg attaacaaga aaataaataa
    22621 tgatcgttaa cagactaagg aacaagttct taccaaaatg ttgctttatt ccttgaaaga
    22681 aaaaataaat gatatttcct gatccctcac ctttccattg cgccattaca taaaaaacag
    22741 cacatgtagc aggcacgata gtaataacag gcaaacacac gattacccag agaataccta
    22801 ataagatgta atcagaaacc acaatcaact gtccataaaa tttagtctca actattttca
    22861 tcttcctcac tctttcattc cagacatagc tgttccttga acaaaatatt tctgtgcaaa
    22921 aatataaacc actagtaatg gcagcattga aataaccgtt ccagccatta caggtccata
    22981 aaaagaaatg ttctgccctg taaataaagc taaccctgct ggtaaagttc tcatttttga
    23041 actacttgtc aatatcaatg gataaagcaa gtcattccaa ctattcatca gcgtgaaaat
    23101 ccctaatgat aaaagagtag gtttgctcaa tggcaacatg attttcaaaa aaatattaaa
    23161 ctcacttaat ccatcaatgc gtgctgcctc ttccaaagaa tctggtaaag taataaaaaa
    23221 ggaacgcatc atgaagatac caaatgcagt tgtcatacga ggaatgatca aacctgcata
    23281 agtatcaagc atgccaagca aattgacttc aataaataga ggaatcatga agacttggaa
    23341 tgggatcatc attgtcagca aaacgaaata gaacaaaact gattttcctt tgaaacgcat
    23401 acgagcaaat gcataccccg caagagaatc aaagaataca gaagtgatca ctgtccctcc
    23461 agcaaaaatc actgtatttt tgatgtaatc taataaaggg atcgttgtcc aaaccttaat
    23521 atagttatct agggtatagc tatctgcaaa tattctaggc ggaaatgaga taatgtcttt
    23581 ttcgtgttta aaagaagaaa taatcagcca caaaaaagga aacgcaataa ccaacaatat
    23641 aactagcata ctaatcgttc gaatgatttt tttaccacta actgcttttc gactattcac
    23701 agtaactgta atcgagcttt ctggtgccat acaggtttcc ctcctatttc atttgttttt
    23761 ctttccgtga catgtacaca ttagtggaga tagctatagt tgcaacaatg ataaacaaaa
    23821 ccgaagacag tgctgatgca tagcccaatt cataaggtgc tgtaaatcct cgactgtaaa
    23881 tatattgaac agctgtttct gtcttaaatt gtgggccacc agcggttgca acatatactt
    23941 ggtcaaacac ttgcatcgaa ccaatcagat tcgttaaaat acaaaaacct aaggagggaa
    24001 cgatctgagg tagcgtaatg ttaaaaaact gctgtcgatt tgttgcacca tccatttgag
    24061 cagattcata caaagaggga gagattccct gaatagcagt caagagaatc gtcatagtaa
    24121 caccaaaatt cttccaaaca gtcatgatgg ctaccgtcgg catagcaagg gttggatcgc
    24181 gaaaaaattg agggatcggc aagccgactt gtgaaaacaa gtaaggaatc gcccctacat
    24241 tcggatcaag caacatagaa aaaataatcg caattgacgt caatgaacaa ataactggaa
    24301 tgtaaaatgt cgaacgacaa aaacgattga atctggtatt tttagataat gctactgctg
    24361 ctaataaacc aacgataatt tgtgttggcg tttcgagtac ggtgaaatat aaagaatgaa
    24421 tcaacgaatt aactgctcgt tcatcactaa atatacgtgt gaaattttca aaacttacaa
    24481 aatttcttga tgtaaagaaa atattcaaat ctgtaaaact aatagcaaat gttcctaaaa
    24541 gcggtatgac agtaaataat aataaaacta aaaaagatgg agttaaaaac aaatacgcag
    24601 cacgatcagg acgattccaa tatttggtga atccgttcat atagtccacc tcatctttca
    24661 ttttgataaa atatacaatc ttggtttaaa cgaatctgaa taagatatcc gaacaatcgc
    24721 tgtgattttt gcaataacac ctatctgcaa atactaggtc gatcattcag atatctgtat
    24781 cttgccaaca gtctatcctg tataaagttc tgtactattt ttgcagtaat tgattgatgg
    24841 catcatctgc atcgttcatg agtgtctcag gatcttctcc tgctaataat ttttcaatca
    24901 gtggattgat gatatcgtta ttgatcgcag agatttgggt aaaacctggt aaatatggct
    24961 ctgcatagtc catttgtttc gataattcag atacaatagg atcatttttt acttcttcat
    25021 cttttgctac tgaatccaag taggcaggaa aaccattttc gatactccat tttttaccaa
    25081 tttcagttgt attccaatat ttcaaaaact catagacagc ttctttttta ttattatctg
    25141 tacttgccgg aatggcatat cctacgccat ctaaaatagc tgtttttttg tctgaatcac
    25201 tcccttgtgg taaggtagtc acattataat caatctcgtt tgctttcaat ccgttattta
    25261 accatggccc attgatttca atggctagtt gaccagcatt cattaaattg tccatttcgg
    25321 cgccagaaat cgattctgga ccagtcttgt ctacatggat cattttttgg atcgctttta
    25381 acgttttaag gttctcagga gacgcaaatt cagacttagt ataatcatcg ttcactaatt
    25441 taccgccatt tgctaacatc cagttatata acggtgcagt tccatcctta ttgaagacaa
    25501 aacctgatac atttttacta ctatcagtta attttggtgc catttctgtc aattcttccc
    25561 aagtagtagg tggttgttca gtatctaatc cagcttgttt aaacaaatca ttattccaat
    25621 acatatacat gccttgtacc tgcatgggga caaaatactg tttaccttct acttcaccta
    25681 attcaatagc cgtttcttta aaatcagatt tatctactcc atcatattcc cagaaatcgt
    25741 ctaatggttg taccgctccg ttttctacat actgtgcaaa atctggataa tttaatgcaa
    25801 taaagtcagg tgctttttta gaagaaatcg ctgtaggaag tttttcattc aaattggccc
    25861 aagtcatgat atccatttca attttgatgt ttttatcatt tgttttatta aattcattaa
    25921 cgatttcttg caatatttca ccatcagatg cagtaaaccc attccaaaaa gtcaaagtca
    25981 cttctttatc agaatcagat tctgctgaac ttccgccacc gcaagctcct aaaatacctg
    26041 ttgcagctac caacaaagag ccaattaacc attttttacc tttcattttt tgttcctcct
    26101 tcatctgata aacgacatct gaccacattc caagatagcg gtttcaataa acaattgatt
    26161 ttcccatctt cagcttttac atttgttgaa tgacttggtt ttacttgttc acttttagga
    26221 agattagttg cttttgtatc aaatccactc atttcagaaa aatcaatcac tccctctaat
    26281 gataattcag acaagtgaac agttagattc atttcttctt ctccacggtt aactgcaaat
    26341 atgacaagtt ctttgtcatt tattacggct atactttcta aatacggcac ttcttcaaaa
    26401 tcttccgtcg catagtttgc tgaacgaatt tgtgggctta acacctctcc ttgtccatac
    26461 acagcagctt gcataaatgg ataatagatc gtctgtctcc aagcttctcc gtttgtttct
    26521 gtcctgattg gtgcaataac gtttaccaat tgagcaagac aagcaatctt tactctatct
    26581 gcatgtttca ataaagtgat taatagacag cctaatagta atgcatcttc aaaattatag
    26641 ttatcttcta gtaaaggagg agcaatctgc caaggcgctg cttgctcatc ttgttccgct
    26701 gaatgatacc aaatattcca ttcatcaaag gacaggttga tctgcttctt actatgtttc
    26761 ttcgccttga ctgtatcgca catcgctgtc acttcagaaa tgaaccgatc catgtctaag
    26821 gaacgtgcaa gataatgaga aagatcattt ttttggttgc cataatattg atgcagagac
    26881 aaataatcta tttgatcgta cgcttgatca agaactgtta actcccaatc accaaatgta
    26941 ggcatttgcc gattcgaact tccgcataaa accacctcaa ttgaatcatc tacgagtttc
    27001 atcgccttag ccgtttcctc tgctaaccgt ccatattctt ccgctgtttt atgtccaatc
    27061 tgccaaggac cgtccatttc attccctaaa caccatgtct taattccaaa agggctctct
    27121 tgaccattcg aacgccttaa atcactccaa taagttcctt taggaaaatt acaatattcg
    27181 actaaattac gagctgcatc tactccacga gtacccagat tgacagccat attgatttcg
    27241 gcccccacat gctgacacca gttataaaat tcatgaatcc caacttcatt cgtttcaatt
    27301 gtccgccaag ctaaatccaa ccttctaggt ctatttgcta tgggcccaat actgtcttcc
    27361 caacgaaatc ctgacacaaa atttccacca ggatatctaa tcatgggaat gttcaattct
    27421 tttaccaagt gaatgacgtc ttggcgaaat ccacttttat tagctgatgg atgatctggc
    27481 tgataaatcc cttcatatac tgctcgtccc atatgttcaa taaatgaact gtacaaccgc
    27541 ggatcaactt tgcctacacg cttgacacta ctaatcaaca ttgtggcttc cataactgac
    27601 ctcctttaat ttatacatga ataatacacc tcgttgaaag cgttgtcaat aatataaatg
    27661 ttaaacttac ttatttttta tcgattttaa ttaaccaaaa tattaataat gattaaaaca
    27721 aataatttaa tataactttt attaacaaat atgttaatat ataagaaaaa ccttttaaga
    27781 ggtgatatat atgcaactac aaataaacga agaatcctta ccggtatatg aagcattagc
    27841 tagtaaaaca aggatcaaga tcatccagct actttccaaa aaaaagatga atgtcaaaga
    27901 tttggcaaaa gaacttggag tgagcagtgc tatcacgacg atgcacgtga aaaagctaga
    27961 ggaagcaaac atcatcaaaa cagaaaaagt aggacaacaa aaaatttcga gccttcgtgt
    28021 cgataaaatc gatattagtt ttccagaaaa aatattcaat gcttttgata cgaaagaaac
    28081 atcgatacct attggtcact atacaaatta tgcaattgaa ccaacatgcg gtttagctac
    28141 gatccatgat tttatcggga aagtggatga accacgctat tttatggatc ctcgaaggat
    28201 ggatgcccgc attctctggt ttaccagcgg atttgtcgag tatcaggcac ctaatttttt
    28261 aaatacagag gatacattgg agatgttgga agtttctgtg gaaatttctt ctgaatttcc
    28321 tttttcaaat gacaattggc cttcagatat cactttcagc ctaaatggtg tggagctagg
    28381 aacatggacg agccctggag attttgctga tatccgcggt aaatatacgc ctgattggta
    28441 tccagataat cttaatcaat acggcttgct gaagacgatc cgaattacca aacacttgac
    28501 gaatatgaat ggagaaccct tgtcaaacat cacgatcaac gacatcccta aagagcaaga
    28561 cacttggcat ttgcgaatcg aagtgaaaga cgatgccaaa catgtggggg gctgtacttt
    28621 atttggtaaa ggattcggta attacgatca ggatatcaaa ttaaaagttt actacagctg
    28681 atatttttct tttctatttt ataaaacaaa aacagccaat ccctatgaag ggacggctgt
    28741 ttttgcttta ttgttatttc cagaaatcat ccaaaacgga aattggcaaa tgtcgtttat
    28801 gttgtgtctt gttccaccat gcttcgattt tttcttgtgc ttgagcggga acttcttttc
    28861 cttctagata atcatcaatt tcattatatg tgacacctag tgcgacttca tctgcaatca
    28921 ttggtttccc atcttcaaga tctgcagtcg gaatttttgt gtataatttt tccggtgcat
    28981 tcaatgcttg aagcaattgt ttcccttgtc gtttgtccaa acggaataat ggcaaaatgt
    29041 cggctccacc atcaccaaat ttcgtgaaga atccagtaat gttttctgca gcatgatctg
    29101 tccctaatac ggctcctgct ctttctccag ctacggcata ttgcgtaatc atacgttgac
    29161 gtgctttgat atttcctttg ttaaaatcgc tgattcccac acctgcttcg cttaatactt
    29221 gtacttgtgc atcaacagct ggtttgatat ttactcgcag actaacatcg ggctggataa
    29281 agtcaagagc agcttttgca tcttcttcat ctgcttgttc accataagga agacgaacag
    29341 cgataaattg atagttttca tcttttgttt cttcacgcat ttcttccatc gttagttgcg
    29401 ccaaacggcc tgctaaagta gaatcttggc cgccgctgat acctagtaca aaagttttaa
    29461 gaaacggata ttttttcaaa tatgctttca taaagtcaat acttttacgt atttcttctt
    29521 ttggatcaat tgttggtttg acacctaatt cattgataat ttctgtctgc aggctcattt
    29581 attctcctcc tatgcttctg tatgaagttc tgctacgctt tttttcattc gttccatgat
    29641 agccatttta tggttccagc attcagtaga cagatcgact gggtattttt gcggattcaa
    29701 gtctcgttta tactcttccc atagtgaatc tagattttct tttgtatatt ctttgatttc
    29761 gtttagtgca ggtaattcgt aaactctttt tccttcgata aagatatctt gtaaaactgg
    29821 gcgagctgta aagtctcgaa cagttttatt gataaacgta tgcactggat ggaacatgaa
    29881 gatagcttct tcttctcgag ggtcttcatc ccataatgtc acgtagtctc cttcagattt
    29941 cccatcaaaa tttcgagtga tgcgccacac ttgttttttc cctggagtag ttactttttc
    30001 tgcattgctt gataacttga tcgtatccat catttttcct tcatcatctt cgatcgatac
    30061 tagcttgaat actgcaccaa gggctggctg atcataggct gtgatcaatt ttgtcccaac
    30121 accccataca tcaattttag ctttttgcat ctttaaattc aaaatcgttg cttcatccaa
    30181 gtcattcgaa gcatagattt ttgcttccgt aaatccggct gcatctaatt gttcccgaac
    30241 acgcttagat atataggcca tatctccact gtcgatgcgt acgccttgga agttgatttt
    30301 gtcacctaat tctttcgcca cgcgaatcgc acttggcaca cctgatttca acgtatcata
    30361 agtgtcgact aagaacacgc agtctttatg ggttttagcg taggccataa atgcttcata
    30421 atcatttcca taagattgga ctaacgaatg agcatgggtc ccgctagctg gtatcccaaa
    30481 tatcttccca gcacgtacat tgcttgttgc atctgctccg ccgatatatg ccgctctagt
    30541 tccccagact gctgcatcca gttcttgtgc ccgtctagtt ccaaattcca atagtggatc
    30601 ttctccgatg acagatttga tacgagctgc ttttgtcgcg atcaacgttt ggaaattgac
    30661 catattcaac agtgctgttt ctactaattg gcagtgcgct aaaggccctt ccacttggat
    30721 caatggttca tttgcaaaaa ctagttctcc ttcacgagca gaacgaactg ttgctttaaa
    30781 ttcaaaattt tgtaaatact ccaaaaaacc ttcaggatag acttctaatc cacgcaagta
    30841 agcaatatcg gaatcattga acgtcagatt ctctaaataa ttgactaacc gttccaatcc
    30901 tgcaaaaacc gcgtatccgt ggttaaaagg catttctcga aagtaacatt caaaaacagc
    30961 atgtaaatca gctcgaccta attcccagta tgtctgcatc atattgattt gatatagatc
    31021 cgtatgcagc gtcaaactgt cgtctgggta agtattattc atcttgtatt actcctcttg
    31081 gcaagttttc atttcctacc cattataaca aaaaaaatta ataggaaaag aaatcttcca
    31141 ttttttctga aaaagcacat gttttacgga tatttcttgc taaaaatgaa ctccatctgc
    31201 taaaactatc ctttcttatt ggtatttaac ctaaacgaga aagaatactt attttattca
    31261 atgtatccga gacacgaaga aattagcgtt ttctattttt cttacttgat aaaataagta
    31321 gattttccta ctacaatcgt agccgttaag ataccgatga ttgaacaaat ataaccaaat
    31381 gtctctacaa cataatcaat ggcaaaagtt tttgaagcaa taatacgtct ttcctttttt
    31441 gcttcttatc cccaaagaaa cggatataac ttattatgta ttgaattttt ttggaaaggc
    31501 gaattatgac aagatatacc ttgcgttgaa aaaactcgcg gaaaaaatat cacacgtttt
    31561 taaaaagata caaagtaaga aagcagctgt caatagaatg gaaacaaaaa tggcgagaca
    31621 tcaatcagtc tcgccttgtt ttttattgta tacattagat atggagcagg ttaccataaa
    31681 gatgattatt agtcgttatt aaatagaatc cttcgtattt ttttgttcta tcactacaat
    31741 atctttattt ggacgtttga attgtattcc ggcacgttct agctcctgtt tcactcctac
    31801 atggactttg acatcttctc gtgtcatttt taaaacgtga tgaatcgcat agtcgatttc
    31861 ttgccttgtg atttttttct tcgtattgat catcacgact tgctttttat catatgtgtc
    31921 taccaatagc agttgtgttc gatctaacga aaataatttg taataattaa tagccgtatt
    31981 cccaaaaaag aattccacta ctttaggata aaatgtctta atatttaaat tactattcac
    32041 aggtgtttcg attagcttca tctcattcct cttttctata tagtcgattt agatccaatc
    32101 ttaagttatg ttaccatctt tcctagcatt aaggaatcgg ttacaattga acctttaaag
    32161 acaaaaggat ttacctttta tcacttaata gtataacata atttcatgag aaaaaaatac
    32221 aattttttct tgaccatctc gttacgtaaa ggattatgct tacatttgtc aggaggaaaa
    32281 ataatggagt acacaataaa gaaaatgtca gagatatcag gagtcagtcc gagaaccttg
    32341 cgcttttatg atgagattgg actgctaaaa ccagctagga tcaattcttc aggttatcgg
    32401 atttatggca aaaaagaagt cgatcgtctg caacacatct tattttatcg aacaatggca
    32461 ttcaagttgg atgatatcca agaggtctta gacaatccat cttttgacca ccagaaagct
    32521 ttaatcaagc atcagcaaat gttgctggaa aaacgggcac aaatcgatac cctcttgaca
    32581 actgtccaac agacacttga tatgtacgaa ggaggaagaa aaatgagtga caaagaaaaa
    32641 tttgaagggt ttaaacaaca aaagttgaaa gaaaatgaag aatcctatgg acaagagatt
    32701 cgacaaaaat acggaaaaaa aaaggttaca gcatccaatg aaaaatggat gaatatggat
    32761 aaagaaacgt atgaagaaat gcaaactgtt gaaaagcaga tgttatttga tctcactctt
    32821 tacttaaaag aaccagaaga tgaaagctta gcagaccgta tcttccaagc tcataagaag
    32881 tggttatctt acagctggcc tgaatatcag ccaaaagcgc ataaaggact aggaatgatg
    32941 tacgaagccg atgaacggtt cactgcatat tatgacgagc gaagtggtca tggtgcagca
    33001 aaagctttaa atgaaattat ccaaaaaaag gcgtaatctt ttacatgatc caacaaaaaa
    33061 gtacacgtaa aatgacctaa taacaaaaca gcagacaccc aagttagatt tttatgctaa
    33121 cttttggtgt ctgctccatt ctttcacaag ttttttcatc ctactttaaa tatattcatc
    33181 gttgaataaa cgtataaggt attttcactt tatccttttt ttctgtaaga aacaattgaa
    33241 atgccatttc tccgcaagct tccagatggt ggtcgatggt tgggaaacca agaagctcgc
    33301 tgatcagcaa attatcgcgt ccaatcacca ctggtgtctt atcttgcaga tagctttgca
    33361 agatgcctgc tgcaaccata tccgaatttg taaagacacc atcaactcca agctctttaa
    33421 aaaagaagcc tgcttgtttt cctttctctg catctataca atcccaaaaa acaagtgatt
    33481 catcaaaaga tggaaaaagt tgatgacata gctgcaaagt gatttttgag ttataactta
    33541 gcctgccact tcgccccaac gtgatgccca attgctttat accttgctgc ttcaaataag
    33601 taagaccttc tgtaattgat tgttctcgat caataaagac acagcttgcc tgttttccct
    33661 tgatttcttc acaaaaaact agaggtccat atttttgata tgccaataaa tcatctagtg
    33721 tattggcacg agacgtaatg atcaatccat cgaaagcttt agctgcaaat tcttcaagat
    33781 accttcgctc tttttctttc tgatagcccg tcggtaaaag tgtcactttg taatcataat
    33841 caaacgctgc ttcaatgatc ccactgatca actgatcaaa atacggatga ttggtataag
    33901 gcagaatcac tccaatgctt ttcgtttttc cataacttaa ctgtctggca tttgcattag
    33961 ggatataatc gacttctttc atgaccgcaa gtatttcttt ccttttttcc tcatctacat
    34021 atggatgtcc attgattaca cgtgaaatag tagaaactga ataacctgtg agctttgcga
    34081 tatctcgaat gtttgccact gctgcttcgc cacctataca aaaagatttt cttgtaaata
    34141 gtctaccaca gctggaccgc attcaaaaaa ctgatacatt tttccttcat gcgaaaaact
    34201 gatccgctgc ccgccccaat atatatcttt tgtcaaattt tcgatttcat tcattggtat
    34261 ttcttttact atattcctat tcaacccttg aaaactgtac aacgttaata cggatccctt
    34321 gaactcgaca aacgcataaa aataattgtt cgttgtgcca tctgttatct ctacataagt
    34381 attcattgca agccctcctt ttgatacttt gagtatagtg tatgaaacgc gtctcatagc
    34441 aaggagtttg ttttgccaaa gaaaagtttt attcctcttt cattattacc aaagtctctt
    34501 attttaaatt gaatacatta taatgatact gtaaatatat ctttattatt agtgaatgga
    34561 tatttacttt taatagataa aatattataa ctattaaaaa taactatttt cattataatt
    34621 acacacaaaa aagagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac
    34681 gtataaacag cggaagcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt
    34741 gaaaagcaat ttccgtgaat ttcttcttat ttcttggagt taaacgcttc tgttccagcc
    34801 tctttttatc tgtctttcac taatgatcag ttgtagataa gcaagttgct ttttcttaca
    34861 tcataccgcc catcattgat ggatccattg caggagctgc tggcgctgct ggttctggtt
    34921 tatctgcaac gactgcttct gtcgttaata acaaggcaga aacggaagcg gcattttgca
    34981 aggcagaacg tgttacttta gttgggtcta cgatacctgc ttcaaccatg tttacccatt
    35041 ctccagtagc agcattgaaa ccagtgccta gttccacgtt cttcaattta tcaacaatca
    35101 ctgatccttc ataaccagcg ttttcagcga tttgacgaat tggttcttct aaagcacgaa
    35161 caacgatctt gatacctgtt gccacgtctc cttctgcttc tacagcagaa actttgctga
    35221 taacgttcac tagagctgta ccaccacctg agaccattcc ttcttctact gcggcacgtg
    35281 tagcattcaa tgcatcttca atgcgtaatt ttaattcttt caattctgtt tcagtagctg
    35341 caccaacttt aacaacagct acaccacctg ctaatttagc taagcgttct tgtaattttt
    35401 cgcggtcaaa atcagaagtt gtttcagcaa tttggttttt gatcaattgc acacgagctt
    35461 cgattgcttc tttttctcca gaaccttcta cgatcgttgt attgtcttta tctacaacaa
    35521 ctttagaagc atttcctaag ttttcgattg ttgcatcttt caattctaaa cctagatcgt
    35581 cagtaattac tgtaccacct gtcaaaatag cgatatcttc aagcattgct ttacgacgat
    35641 cgccgaaacc aggagctttt acagctacta cgttgaatgt tccacggatt ttgttcaata
    35701 caagagttgg caatgcttca ccatctacat cgtcagcaat aatcaacaat ggacgtgatt
    35761 gttgcaagat ttgttctaac aatggcagaa tatcttggat gttagagatt tttttgtctg
    35821 tgatcaagat atatgggttt tctagaacag cttccatttt gtcgttatct gttaccatat
    35881 attgtgataa gtaaccacga tcaaactgca taccttctac aacatctagt tctgtttcga
    35941 tcccttttga ttcttcgatt gtgatgacac cgtcgtttcc aactttttcc attgcatcag
    36001 caatcaaatg tccaacttta tcagaacccg aagaaacagc tgctacttgt gcaatggctt
    36061 cttttgaatc aacaacagtt gagatattat gcaattcttc gactgctgct tttgttgcca
    36121 attcgatccc gcgacgaatg cctagaggat tagcacctgc agttacgttt tttaaacctt
    36181 cgcgaacgat tgcttgtgtc agaacagttg ctgtcgttgt accgtctcct gcaatatcat
    36241 ttgtttttga tgctacttct gatacaagtt ttgcacccat gttttcaaaa tgatcttcta
    36301 attcgatttc ttttgcgatc gtcacaccat catttgtgat caatggagag ccgtaagatt
    36361 tttctagtac gacatttcgg cctttaggtc ctaatgttac tttcactgta tctgctaatt
    36421 tatctacccc gcgtaacata gctgcgcgtg cgtcttctgc aaatttcaat tcttttgcca
    36481 tgattgattc acctcattga ttatttttga ttattttttt atcttccaaa tttactcaac
    36541 aatcgccatg atatcttttc ctgcgacgat cagatattct tttccttcgt attttacttc
    36601 tgtaccggcg tatttttcga acataacttg gtcgcctgct tttacagcgg caggaacttt
    36661 ttcaccgttt tctaacaaac ggccttcacc gactgcaaca actgtcccag tttggggttt
    36721 ctctttagca gcagaagcta aaacgattcc tccgacagtt ttttcttctt cttttgcgac
    36781 ttcgataatg acgcgatcac ctaatggttt taacacgata aatccctccg ataactcttt
    36841 ttttaatctg ttagcactct taaccttcga gtgctaacct acatttttaa taatactcat
    36901 tctccctctt ttttgcaagc ttttaaatca aagaaaagaa attttattct gacagctgct
    36961 tattgaaatt atgatataat tgaaatcaat cgatataagc ttgccttgca tgcttgttat
    37021 cccttaaaag gaggaattac atgtctttaa aaaaatatag cttcctcagc attttttgct
    37081 atggtctaat ttttatctca cccttgatcc tagcttcgat aggacttgta aaatctacgt
    37141 ctgaattgat cacagcaact gcagtcatgt atatactagg cgccgttgtc ctagctattt
    37201 tttattttaa gcaacgtgaa cccttagcga tcgaatcagc ggtaaaaaaa gcttctgctc
    37261 caaagattgt tatttatgga ttggttggaa tctttgtcgc attgattctg caaagcatcg
    37321 ctgtcatgtt agaatctgta ttgtttggca aagcaacccc ttccgagaat acacaaaata
    37381 tcatccagat gatcatggaa gcacccgctt ttattttggc gacaactgtt gctggaccca
    37441 tcatggaaga gttcgtattc cgccgcagta tactggggat catcagtcga tattcaaatt
    37501 tttgggtcgg agcagtgatc agctctttgc tgtttgcgtt tgctcataat gatggtcatc
    37561 tgctaatcta ctttttccta ggtttcttct tcagcctaga gtacaaagca actggtcgga
    37621 tctggacatc gatgatcaca cacgtaggaa tgaacacatt agttgttctt gttcaattag
    37681 ctgtccaaaa agggctagta taaacatgac tataaatact cctgtcatcc tagattggtg
    37741 attttcccaa tctaaagcga caggagtatt tttgtttttt agtcgttcat cttttctgct
    37801 gctccatcat tttttgtctg tctttccttt ttgcagtttg cagaatctat ttttttcaaa
    37861 aatgggaaat aaatgaaaaa actaattgac aacacgatac cttgtaaaat cgctgcaggc
    37921 actccgccta ctaagaaacc cgagaggatc ggcggtgttg tccaagggac ttggactgct
    37981 gtaaataaag gaacgaatcc aaaatataaa gcggaatagg taatcaatcc tgaaacgacc
    38041 ggtgcgaaaa taaaaggaac agccatcatc gggttcatca cgatcggtgt ggaaaaaata
    38101 attgattcat tgatgttgaa aaaggctggt aaaagagcaa gatttcctaa ttctctgtat
    38161 ttcttcgatt tcgcaaaaac cgtcataaaa aatacagccc ctaacgtcaa tccagcaccc
    38221 gtaaccgtca tgaactgatc gagaaattgc tgagtaacga tatgtccgcc atttgccacc
    38281 gtcaactctt gtcctgaggc taagatttct gcatttgcta atgaattcgc ttgcaagacg
    38341 ggactaacca acccattgac catcgcagat ccatgaagac caaataacca taaaaacgga
    38401 acaaaaaaac ctaataaaag cacaccaaac aacgtgtcta tgaacgattg aatgggaagc
    38461 tggacatatg tccatactcc ttgaacaaca gtcattctga acaaatgttg aaaaaccgct
    38521 gaaccgataa gcgtaaaagt gactataaca atgattggaa tcaatgaagt aaaagactgc
    38581 caaacatttt caggtatctt ctctctttgc ttccaccctt tcgcaagtct tactaaacgt
    38641 gcatagcccc atccgatgat gagcccagtt gtaactgctc ctagtatccc ttcagcacct
    38701 gtccagacag tatctatctg agtatttagt ggctgaataa gcagtagata agcagacaag
    38761 gctgttatgc cgactggtaa gcgatcgatg tcttcttctt ttgcataagc gtatgcaatg
    38821 gcaaaaacga tccaaaaggc tatcacatca aatgtagcct ggtatacttg tgtaaaatac
    38881 atagaaaatc ctgttttgtc tatccactga gcgatgtaat ccacaggaaa atgagcaaat
    38941 aagaaaaaca gcgagccaac aataattatt gggatcgtgt aagtcattcc attacgcaaa
    39001 gcagttaagg gtctaagatt cacaacagat gttatcattg gtaaaaaggc acgatctaac
    39061 cactctttca tttcttatct ccttcaactt ctatttttat caatcagttt tcttaattta
    39121 actataccca atgatcacga accatgaaag gaatgcgcac tgagcttttc aaaaatcgga
    39181 ttctttgacg aatattcagc aactgcttac aatggacagg aataaaaaag gaggtttttt
    39241 atgaaaattg cactcattgg agctactggc catgcaggtt catttatttt agacgaagca
    39301 ttgtccagag atttagacgt gacggcaatc gtgcgtcatc cagaaaaatt gccatcagat
    39361 gttccgttta tacaaaagga tttatttgag ttgacaacta ctgatcttgc atcttttgat
    39421 gttgtcgttg atgctttcaa cgctcccaaa ggaaaagaag aaatgcatca aacttctttg
    39481 tcccatttga ttgagatgct tgaaggaacc gatacacgct taattgtcgt tgggggtgcc
    39541 tcttcgttat tcttagatac agaaaagacg actcgtatga ttgaccaagt acctaaaaat
    39601 gcacccttct atccaacagc tttcaatatg tacgaaggat tgttaaaatt gaaacaatct
    39661 aaaaatctac attggactta tatcagtcca gcttctttat ttgatccaca tggcaaacgt
    39721 acaggtactt atttgttaag tgatgattat ttacaaagaa acgctgctag cgacagtacg
    39781 atcagcatgg cagattacgc gattgcatta gtagatgaga tactggatga gaaacatgaa
    39841 aagcagcaca tcagtgtagt cagtcgataa tacggttatt tttttaatat gctaaaaaaa
    39901 taaaaataga agataaaacc aaccctgatt gattttatct tctgcttttt atatttttat
    39961 tgattgaact gtttaaatcg aaaatagctc agttgcttca tctggatctg tatcatagat
    40021 ttcaaaatca tgtccaacgc caaaatcttt ttctttcaaa acattttcca ttgttaaatg
    40081 agcaagttct ttcatattgt tttccttgct aagatggcca agatagattc gtttggtccg
    40141 attaccgatc acatcagtca tgacaagtgc accgtcttcg ttggaaagat gtcctctatc
    40201 tcctaaaata cgctgtttta aattccacgg ataaggtccc atgcgtaaca tctcaagatc
    40261 atgattactt tccatcaaat aaccatcggc attttcaatg acacctcgca tataatcact
    40321 gcaatatccg gtgtccgtca acatgacgaa tgaccgattt tctttatgga aacgataaaa
    40381 ttgcggagcc gctgcgtcat gggagacgcc gaaactctca atatcgatat cgccgaatgt
    40441 tctgactttc cccatctcaa atagatgttt ttgttccact ggtacatttc caatcagtgg
    40501 ttccatcgct tcccaagtct gctgattggc aaaaacatcc aaatgatact tacgagccaa
    40561 gacacccacg ccatgtatat gatctcgatg ttcatgcgtc acaagtattc catctaattg
    40621 gtccggtgtc cgatcaattt gagccatcaa ggaagtgatt tttttaccgc taagtcctgc
    40681 atcgaccaaa agccgttttt tttcactttc gatataaagt gaattacccg aacttccact
    40741 tgctaggata ctgattttaa aagctggttt ttcttcagcc atttgactcc tacttccctt
    40801 cttactttgt aaactttttt cattatactc gcttttttat tgatcttcca cttttggtac
    40861 agtattgttt gtcacgatcg tattgctgaa agcgttgatg ctctcaattt ggaccacttt
    40921 atcgcttgtt tcgatcgcta catgccaaac aggaacatac acattttttt cccggacttt
    40981 cagaatacag gaataagcta gctgacgcca taaaattttt gatcctcttg gtattttgtt
    41041 attgatgtac aatgtattga ttgcttcctc ttccgtatac agttccgtct tttcgcgcag
    41101 ctgctcgata tcagataaat gcgtctgcat gtatttcgtg atgctataat cattatcgtt
    41161 tttttctaaa gaaagaacga tttttgctgt atcatcattg aatgggatgc cttcatactc
    41221 ttggcttgct tggatttcta gtggttcttc ttctaaatga gaaaagtttt tcgtgtacac
    41281 ataatcatta ccgaataaaa tattgtcttt gttagataaa aaatcttcca aggaactact
    41341 aattttatct ggatcgatca cgttcttttc agcatctgaa ctggataaag atttcgtcaa
    41401 aacatcaccg tctaaaaaca caccacttgc cagcaagccg cttttaccag ccttgcgata
    41461 attcgctaat gaagtgctaa gattggtctg ctccgcgcta agataatagc cttgcttgtt
    41521 ctcagacgaa atcttccccg tgtaagtgat atcgtcgcta gccagacgtt gttggattga
    41581 ttcagtctga ttagagcgaa tgacattgtt ttcttggtat accgcgtcat gataactgct
    41641 gaataaaaac acattcaacc ctaaaaaagc gacgaaaaat atccattcga tttttttgaa
    41701 atccaattaa ttcgcctcca atccaggtaa tttttccaga aggtcgttag ctgaatacca
    41761 gttatcttta tatttcacat accattcagg taagagatca acgacacgat tcgtttcttt
    41821 gacattttgc catgtatagc caacgatcac tgaaccaatc ttgttgctat ctgctccctt
    41881 agcttttaac tgctggatga tttcattcgt acttgcaagt tctacttctt cgtctgaagg
    41941 tatcggcact tgaatcgtat tcaaactggt ttggatattc acttgcacag aaccattcgt
    42001 ttcaccgcca acttccacac caatctgtcc tctgctgtct gtcccaaaaa ccgggaagcc
    42061 ttccacgaat atccgataat cgatcgtatt gtcactgcgg tcaaaatatc tgaggttccc
    42121 taaagaactg cctaatttgc taatataggg gaaactttca gaaaatacac tagcattttc
    42181 ttttttctct ggcagctctc cacgaaaatc aactacttgc gtttccgaat ggatagacat
    42241 tgtttcattc ccaccgtaaa aaatcaattc attgctttca tcattatttt tcacgctatc
    42301 tggtttctgg aaaaaagcat ttctaaaaag tgtatagggt tgctgtgaca ggatataact
    42361 atacttcttc agtttgatcg aatgtttcgt agcatattgc gtgtcgatcg tcgatggttc
    42421 tgttgtcatt tctgtccatt ttgtgtcttt tgctgttaat tctttattga tatctgtcca
    42481 attcaacgaa atatttgctt catatacgag atggttcgaa taattcaaaa atcggatttt
    42541 tttatttcct tgatcgaatt gtattctagt aaaatacata ttttcgcttt cttttgccaa
    42601 gttcgacaca tctaaatcta aatcaaatgc ttccttgtat tctcttagca aaaaaggcgc
    42661 attataggcc aactcaaaac cagagttcag atttttcacc tgttcaaact gtgatttgtc
    42721 tcctgaaact acttcagaaa gctttccaaa tcttgcttta tcgatgatca attgcaatcg
    42781 gtacacgaga ttttcactgt tggtttcttg gatcattccc gaatggtgcc atgtaacatg
    42841 aagcggaaga aagacttcgg aagtttttcg attgctttga gactcgatca tttgagtgtt
    42901 tgtctcatct acgttgattg aactttttcc tgctgggctc agccagattg cataagagaa
    42961 gtatatgctt aacgcaacca tcaaaatgag tcctatacga acgacttttt cagagattct
    43021 cattcccacc attcctcctc ataaggttca tatggcagcg agatataaaa agtcgaccct
    43081 tttccttcct tgctctctgc ccaaatagcg cctcgatgcg ccttaatcac ttctctagaa
    43141 atagctaatc caagtcctgt cccaccttgt gctcttgcac gtgctttatc tactcgatag
    43201 aaccgctcga acactcggtt cagatctttt ttaggaatcc ccaatccttg atcggtgata
    43261 cttaaaatca cgttattatg cgtttctaat aagcgacaag taatcgttcc cccatctggt
    43321 gaatacttga tcgcgttatt catgatatta tcgactactt ggatgatttt atctgtatct
    43381 acttcgaccc aaagatctct ttgcgtaaat tcacgacgga tcttataatt tttttcctga
    43441 tttcccacca tcatatcaaa ccggtcaaga acaaaattca ctaactcgtt gaaattgaca
    43501 tattctaatt gaagctgggt attacctgta tccatgcgag atagatttaa gaggtcattg
    43561 atcattcgaa tcattcggtc tgtttcatct agcgttacct tcaaaaaatt tggtgctact
    43621 tcctcgtctt tccacgcacc ttcgctcaat gcttcgatat aactacgcat acttgtcaac
    43681 ggtgtacgaa gttcatggga aacattagag acgaattcac gacgttcccg ttctgttttt
    43741 tcttgttctg tcacatcatg caagacagct actaatccgc tgataaaacc tgattcgcga
    43801 cggatcatag aaaagtctac tcgcaagatc atctgatcac tttccaatgt actggttgaa
    43861 cggtcaagta ccatttcgtt tggatcttct aataatttcc gcaacgtata ttcttcttcg
    43921 atatctaaaa gtgtcaaaat cgattgtccg attgcttcct cgtttttgat atctagcaag
    43981 gaaacagcca tatcattgat cgtaatgact ttacccctgc gatctgtcgc aatgacgccg
    44041 tctgtcatat gggatagcac gctatccaaa cgattgcgtt cagcttccat tgtatcttga
    44101 gcttcttcaa tacgttcagc taactgattg aaggtatcag ccagctgtcc aagttcatct
    44161 tgtccatgca ctttgacttt ccgactgtag tctcctctag cgattcgaat ggcttgttct
    44221 ctcatttcac cgatcggctg tgtaatcgaa cgagcaacta aaacagatac gatcattgaa
    44281 ataaatgccg caattagtga ggcagtaaag aaaatgactg ctgtgttatt gatttccgaa
    44341 tatttttgtt cgatattact tttgacgtaa agagccccga taacggcatc gccggtcggg
    44401 gacaggatcg gttgaacatt gatattcacc cgtttatcgt cgttatctaa agcaacataa
    44461 cgtttagtcg tgaaatcatt gatatcaaca taatcatttt tctttcccac agcgctttgt
    44521 tcattgacat cggttgttcc tcggatgatc cctttttcgt caacgacccg tatttcgatg
    44581 atatcgctag tcgcactgtt ttcaataaat cttttgatat tcgcatttac ctcttcatca
    44641 tctgcattat cttttcgatt caattccggg ctgattgttg ttgctaatga ttccaccgtt
    44701 tgattcatat ctttaataaa tgtattgatc gtggaacgtt ctaaaccacg tataaagtag
    44761 gcaccaataa tttcgatcga gatcaataaa atcagaataa aggtaatagc gatcttaaaa
    44821 ttgactgatc gaaaaaagcg gacttttctt ttcatccaat tactcctgtt ctgggttacg
    44881 caaatagtaa ccgacaccac gacgtgtcac taaataattt ggatggcttg gattatcttc
    44941 gatcttttca cgcaaacgac gtacagttac atccactgtt cgtacatctc caaaataatc
    45001 ataaccccaa accgtttgta ataaatgttc tcttgtcatc acttgtccaa ggtgcttcgc
    45061 caagtaaaag agtagttcga attcacgatg tgttaactcg attgtttctc ctcgtttcgt
    45121 caccatgtaa gcttctggat ggatcgtcaa atcaccaatc gtcaattccg caggtgctgc
    45181 ctcttctggt tccttcgcag ccgtggcacc gcgacgaaga tttgctttga cacgtgcaac
    45241 taattctcgg ttggaaaatg gcttggtcac gtaatcatcg gcgcctaact ctaagcctag
    45301 gactttatca atttcagaat ctttggctgt taccataatg atcggcatat cataagtttt
    45361 gcgtacctca cgagcaactt ctaagccgtc cattttaggt agcatcaaat caagtaaaat
    45421 caaatcaggt tctacctcag caactttttc caaggcttct tccccatcat atgcagtata
    45481 aacgtcatag ccttctttgg ctaaattgaa tttgacgata tccgaaatcg gcttctcatc
    45541 atcgacaact aaaacttttt tcataaagag ccacctctta tatattattg tcataccttc
    45601 tcattatacc gtatttctgc ttttgtttca tctttgccta taaaacaaaa gagaaaatta
    45661 aaatgaaagt gcaaaatact ttattatttt ttgttctaac ttcaataaaa aaggaaaaca
    45721 ctgattttca cctcagtgtt tccttttttt gatttatcct tctaaaactt gtacaccgac
    45781 acccaataat cccggaccag tgtggactac taatgccgga gaaatctggc caaagtagat
    45841 ttggtttgct tgtgggaact ctcttttaag tctcgcttcc atttcaattg cttcttcttt
    45901 cgcatcccca tgggcaacag ctaagttgaa gacctttgca ttaccgattt tttctttgac
    45961 gtaagcaacc gttttatcca aacttttttt acgtccccta gcttttccta ctgtgtaata
    46021 aatcccttct tcattacatg agatgattgg gttaagcttt aaagcggttc ctacaataga
    46081 agcaactaaa ccgatccgtc cacctttttg aagatattcc aatgtcgcaa cattaaagaa
    46141 aacttttgtt ctttcgacta actcattcag attactgatc agttgatccc attctactcc
    46201 ttcttccagc cattttgctg cttggatcgc agaaaaacct gcaccaatac caatattttt
    46261 cgtatctagt acatatgctt ccaattcttc ttgctgttgt gcaattaaac gaaccacatt
    46321 atatgtcccg ctcaatccac tggaaattgt taccgcaagt accttttcat aaccgtcttt
    46381 tttgatttgc tcaaagattt ctgtgatcga ctcaccatcc ggcaaagatg taccagggat
    46441 ttcctctggc aaacgttgat agatctcttc aggggtaatg tcaattttat ctgtatatac
    46501 ctgatcttta taaataattt tcaatgggat catatacatc tcgtattttt ttatcaattc
    46561 ttctggaaca tccgtacctg aatctactaa aattgcgatt ttattgtttt tcatttgttt
    46621 tctcctcttt taaatattcg gtttctagaa caatcgtttt ttccgcaaga agtttcattg
    46681 caaacgataa agttgccgct cttacagcaa tatattctac aggggttgcc tggtcgaatt
    46741 tttgcggatg agatttacct tgtgctaatt ggctgaccat ccacacagct gcttcttgtt
    46801 catcgcaaaa tagattatag gcttcacgaa ttcctactgt ttttccttgg aacaatatcc
    46861 cttctttgat ttcaggaata gtcaatactt gtttcaacgt agtgattgca agtaaaaatg
    46921 ctacgtgttc tttgttatat ctttttttaa ctggtgctgg tatcaaacct agtttaacgt
    46981 aattgtttac catagaagag gtaagcaatg gatgcttttc tccttgtatc acaggtgaaa
    47041 gataacgatc aactaacgtt ctgacttggt ccatataaag ttctaagtca gggagttctt
    47101 cccagtgagg cagacggaat gtttctagtt ccttccccca ctccattaat tgctgacgca
    47161 ttttttccat cataaccgct ccttttttca tttcataaac atgatatcag acttatgata
    47221 aacattcaat ctagttgctt taactaggtg atttgattag cgggattcca tgaaaacaaa
    47281 aaacaagaag gctaagcctt cttgtctttt atcgttaaga acaggagacc cgccggggag
    47341 acggcgggaa aggagtaaaa aatgaaaaaa gtgttagggt ttgttctctt gcaagaactt
    47401 tgtttgttgg tatgcttata taatagcgag caaatgtgaa taatctatga atgatttcct
    47461 ataaggaagt aaagattttt ctgattttct ttaaatgtag tcttgcattt taaaattctt
    47521 agtgctacta ttattaagta ctttttgtac gggccgttag ctcagttggt agagcagctg
    47581 actcttaatc agcgggtcgc gggttcgagc ccctcacggc ccattgggtg ccaaacccac
    47641 ggaacaggta aatgttggtg cgaaagcatt tcgaaaacat ttacgctgtt ctttttttat
    47701 ttttcaaaat aactagggct ttttattttt aactcaaagt ccactgtagc cctttggact
    47761 ttgagttaaa aaagaagcat aattatgaac agtaaagttt ctaatataac aataattcga
    47821 gaagctttgc aaaatcatgc tgccaatgaa ctctctaaac atataggatt aaatctaagt
    47881 actataaaag gttaaaaagt gaagaatatt taatagaaaa actaaattta cttgctgctt
    47941 tccccttttc cattaataca acaatcagag agtgtctttt tttcgaaaaa attagaataa
    48001 ataaaagaca cgtagttttt ttatctacat gtcttccatc gactacaagt tatcatttct
    48061 caaaacgtat ttagctttta attgattaat cgtttcacgt tgtctacaca ttgcctaatt
    48121 aagattttct tattagaagc atcaatcccg caataatacc taaaactcca ggaattaccc
    48181 acaataatgt taaaagaaaa aatggaataa tagataaaat gagtaaacta ataccataga
    48241 ccactgtttt ttgatttttt cttactaaaa aacctattat gaaaatcatt atcgaaaaaa
    48301 ctagggtaat tcctccaaat aaaacaacat tcccgcttag attgactaac atatctatat
    48361 ccttactggt tagttctgta gtatttaaag tagttgaatc aatagaagat agaaacatac
    48421 cacccatcaa tgctaaaaga ctacataaaa tccctgttat tccactaatt aaaatcaaaa
    48481 taaactctgt ttttctctta ttcatttaat tctctccaga ttaagtgttt tagaattaga
    48541 aattttttga ccatagttaa tagcttctaa tgaacgaata acaaccactg acgtgttaga
    48601 aaaataatcc ccaatatgtt gtttcttggg cgtgaaagca gccaccaaat acccaatttg
    48661 aaatacaaaa ttttttaata taaacctaca tatacactca cgaataaaga tagtttgcca
    48721 acttagctct ttttcctcaa agctcactac tcttatccca aaaatcattt tcccaattgt
    48781 ttgtcctgaa ttcagcttag tcaaaagtac aaaataggtt aaataaatag ctaatgacgc
    48841 tataccataa aaagtaataa tggatttgtc agattccagt cccatgattt tgtaaaagag
    48901 acctatagta gcatgggtga tgatgctaat acaaattaaa tcaataagaa aagcaaaaaa
    48961 tcgtaaccaa aatcccgcaa atacataatt aggcaaagtt tcaatgccac tcttcttatt
    49021 cattttatag ccctccatag taatacattg gttttggagt atcagaaaat ccaagttttt
    49081 caaaaactga aattacacga tctgatgtag ttgcctttag cccttgccat tcagcaatct
    49141 tattacctaa ccaagaagaa gcaaagccag ttgtgtcatt tttatattct ataaccgtcg
    49201 catcagttaa tttcttctct ttttttaggg aatctaatgc atcttcagag taaccaattt
    49261 gatccaccaa cccattttct actgcttggt taccatcata aattcttcca tccgcaagtt
    49321 ctttcactgc attagtatcc atatttcttc cttttgctac aacattgaca aaacgatcat
    49381 aagcactcat cacaaattcc tgtataactt tattctcttc ctctgaaggt ttgtgttgag
    49441 gtgacatcat atcttttaat gcaccacttt tatacgtcgc atcagttacg ccaagttttt
    49501 ctaataagcc agaataattt aaaccagaaa taatgacacc aattgatcca gttgttgttt
    49561 cttccgtagc aaatatttta tctgaaccag cagaaatgta gtaccctcca cttgccgctg
    49621 tatttttaaa tgcagtatag ataggaatat ctagcttttt aatttttgcc atttctttag
    49681 caatctcagc actttcatat attcctcccc caggtgaatt tacttctaat aacactccct
    49741 taaccgcttt gtcttcttga attttcttta gctgtgttaa aaaattctga tggttatatt
    49801 gttccctact aaaaagattg ctttcacctg tatctgcaat aactccattg acggatagtt
    49861 tgacaatctt tttatttgat gcgccttctt ctaacgtttc ttctgtgatt tcgttagatc
    49921 cgtaaaaaat cttatttatt cctgttaggg ttgtatttga tgcttcctct ttatcttttc
    49981 ctggaattag cgcgacgata atggatagta acaataatcc gcacgctact cccacagcaa
    50041 tccatcttct tttattcata taaactcctc ctatattctt agtaaaaata attatttttt
    50101 atttagaaac acatcgacaa tttgttgcca atatgttttt tctcctacaa ttaaagacaa
    50161 ctctccagtt aatccataac gaaacggaaa cgattgagtt gtttttaacg ttcctttcac
    50221 tgcataaaaa acacctttct cagtggcagt agaattttct gaaatttcat ctaaagttcc
    50281 gtcgatcgta atcggagccc cacctttttg atccaattta aaatgaacct tcatcccttc
    50341 tttaatatag gtactttcat tcgcaggtaa aagtgctgta aatttcattt ttgatttttc
    50401 gtggatcgga taaatttctg caagcactgt tcctttggct agttctgctt gtccctttgt
    50461 ttcttcattt aaatgaaccg ttccagatac aggagctttt agtattcctt ttgaaagttg
    50521 atcgtccaaa cttttgataa ctgactggtt cttctcttgt ttttcattta gctctgtaac
    50581 tgtttgtttt acagttgtta ttgcttgttc tagctcttgc tttccttttt ccgtttgact
    50641 aattctgtca ttctcagaag tagttggttt aactaattta gctttttctg actgatactg
    50701 ttctacttct ttttttagtt gctcaatttg ttcatttatg cttgctgaaa ttgtcagttt
    50761 cacttgattt ttttgatcat cagaaacatt atttagttgt tcttgccaat tctcatagtt
    50821 tgccatcatt tcttttgaaa aatcttttaa tgattgatta ttactccaag ctacctgtac
    50881 ttgttgccat tcttgtaatt tactatctga actctcaatt tgtttttcta gttgtctttt
    50941 tgtcttttcg tatacttcta attgcttttg atcatttaat tcactttgct tcaaagcgta
    51001 gcggagtgat tctttttctg acaacatgct ctttaattga ttactgtagc caaaactgtc
    51061 atccgttgaa aacaaatttt ttcctttact tatactttca ataaatagct gagccatctt
    51121 tatttgttga gtaatacgtt ggttttcttg ttctatctga gccttttcat tctctattaa
    51181 agaacaatcc aatctaacca acacatcgtc cttatgaaca aattgatttt cttttaggaa
    51241 gttttccatt attttagctt ctaatggcac ttgtaacttc tctatttttt cggggttcaa
    51301 ttgagccgtt gtccgaataa caacctcttt tttagcaaag gttaaaaaca atccaataat
    51361 acataaaaaa agaattacag ggtataacac ccaacggtag aagctatgat gttgtttagt
    51421 atagatacta gaatgttcga accaattatt attctgcata aattgtttcc ctctcctaga
    51481 tttcccataa tttttgatag ataccatcgg caaaacgaag ttcatcgtgc gttccttttt
    51541 ctaccaaatg cccttcatgg agaactaaaa tttgatcaca tgcttttgct attggtaaat
    51601 gatgcgcaat aaaaataatc gttttttctt ttaaattgag gagataattc ataattgcat
    51661 gttctaacaa ggtatctaat ccactagttg cttcatctaa aattaatatg tccggatttt
    51721 ttaataaagc ccgtgctaag gctaatcgtt gtcgttgccc tcctgaaaga ttatttccac
    51781 cttcttccaa caatgtctcg taacgtaatg gctgttgatt aataaattca tttagttgga
    51841 ctatctcaca aacttctatt atttgttcga aacttggtgg gctatctaaa ccgaatataa
    51901 gattctcaat gattgttcca ctaaagaaaa atgactcttg aggcaaatat gtcactcgat
    51961 cgcgtaatat ctgaaaagga atgtctaagt gattaatgtt tccataatgg atcgatcctt
    52021 ctgatgcttc ataaaaacga acaaataact ttgcaagagt tgattttcca gaaccactaa
    52081 ctccaactat agccgtttta ctgtgcgaag gaattgtaca tgaaatttcg ttcaacgttg
    52141 gggccttcat gctatacgag aatgaaacgt tttttacagt gatatcttgt tgaaataatt
    52201 gttcagaaag catatattca aaagactggt tcgatttttc tgaatcaatc gcgaaaattt
    52261 catttaaacg attatttgca acttgagccg tttgcatttt cacttgtaaa ttaataatgt
    52321 tttgtaaagg atctgtcaaa aatactagta aagcattgta tgtgataagt tgacctagac
    52381 taattctatt atccataaca aaataagaac ctagccataa aattaatgca ctgctaatta
    52441 attgaatcaa atgtttaatt ccttgttgaa tattatcaag cgtaaccgta cgaaatgatt
    52501 ttttcattaa tttaacaaat tcatggtcta tacggttata tactttctct tcaccgctat
    52561 atgctttaat tgtttctatt cctttaagac tttctatgat actagaattt acagctgcac
    52621 cggctgccat ttcttccctg ttcgattttt catagctttt cacgaaagcg aatattgcaa
    52681 ctacataaaa aggcaaagag catagagtga ttaaaaacaa cgtactattc tgaatagcta
    52741 acgtaacccc aacaagcaac accataccaa tatctaaaaa tattgataac gtagcacttg
    52801 ctaaagcatc aataatctta ctggcatcaa gaaaacgaga aataatttcc cctgattttc
    52861 tcgtagcaaa aaaattcatt ggtaaagtta acacatgttt gaaatactga agcataattg
    52921 ccatactcat tcgctgtcct aaaataataa gaagataact acgactatat tcaaacaata
    52981 cccgaaataa atagacagcg atcaagccaa tggaaattat atttaaagtt gaacgtgctt
    53041 gattagggat gaaaaaatca agaatacctt gaaaataata tgagcttcct atgccaaaaa
    53101 acgtaatgaa aatcgatgct aaaacgatgt ggaatactaa tcctttttgt ttccaaatta
    53161 taggcaaaaa agaacttaat ccagctactt tttctttaat aggaatataa gtttctctag
    53221 gcataggtaa taaaagaata cctgtccatt ctttttgaaa cgcttcaatc gtttttctat
    53281 tttttccatt agcgggatct gcaatccata gtacgtttcc cttcgtttta taaattacaa
    53341 cgtagtgcat atagctgtta tcaaccaata tatgtgcaat taatggtaag ataagatctc
    53401 tttctttcca gatctcacta tctgcttgaa tagccataca atcaaaagac aatttttcaa
    53461 atgtcttttt caatccaaat gctgaagtac cctctaaatc agtcccagat aggtcccgta
    53521 atttatgaat cgggatatca gttttaaaat attttaaaat catcgaaagg caagcaacac
    53581 cacaatcttt ttcatcttgt tgctgcacaa aaggaaatga ttttatcact tatctaacac
    53641 aactccttct aaaaaatttt tatagaaaaa ttctcaatat agctttaata cagtgaaaat
    53701 attgtctcat aattccacaa aaactttgaa aacagttctt gaacactaac tttttcattc
    53761 ctgaatcaaa tttattttgg aaattttact attcaagaat aaacttgata agtttcagga
    53821 agatttttac tgttaatcaa atacgtatgt tacagtagca ccgtaaaaaa tattatttaa
    53881 agaagcgtgt ttaaacatgc aaaatgtaaa ggaactaagt attgtggaaa tgcacaaact
    53941 gttggtggag gcaataatgc ttggggaaaa ttggggcaag ttgtaggtgg acttaccaca
    54001 ggggcagtag gtggtgcagg tttaggaaca gcaatttgcg gaccagcttg cggtgttgtt
    54061 ggagggttat atggagctgt cgctggaggt gctgcagctg gctgggatgc tcgaaaaaaa
    54121 taaaaaagga gaataagtag tatgcatatt aaaaacacaa aaacaacatt tatattatct
    54181 tcagaagagt tgaaaaatat tcaaggtggg agtgccgtgg gagttttagg cactactttt
    54241 tctggggcaa cagcaggagt aaaactttgt tcagccggag gaccttatgc aatagctgct
    54301 tgtggtgtag gtggagcttt attaggtgct ggattcagca tgtggaccgg tgcgtaaaca
    54361 attaaaaaat atcaaacctc aagattggtt tgtgtcgatt acgaacatag gtatgttctt
    54421 tatcttttca aaagtttgga atggacagaa ctttttacta aagcttcttg tgctagtttt
    54481 tataattata ttaaattttt atcttttatt aaagtcagat aaaaattctt tggaaatgta
    54541 atggtagaga tatttttaca aaaggacttg cagcgctgta gttggtgctg caggaggacc
    54601 tatttcggcc gttacaggag gtattggtaa tgtacttgtc ggatgtgcat taaagcctgt
    54661 tcctgcaagt taaatttata aaaacaggaa agtaattatc tcaaatccta tcaagtacaa
    54721 tcataggatt gcacttgaca ggacaaattt tttaactaaa aaatgaaaaa cactttctaa
    54781 aaattagtag aatttgccaa atctttcgca tcttccacca tacatttaat tttaattccc
    54841 ctttatggta cttaataaaa acaagaaaag aactattcat ttggcccttt tttggttcca
    54901 gaccatagaa atattcctcc agtaactaat attgctgtta tgcacgtaac taataacagc
    54961 tataaaaatt ggtacaactt taataggatt cagaaaaatt tgcactagtg aaggaatcta
    55021 ttttattaga aaaatgcaat ctccctaaag aaaaaaccac taaacagtgc tttctttcaa
    55081 cttctcctta attttttgtg ctttccctaa agtaaagttt ctggccaact ctgaatcaaa
    55141 atatagtgaa cgttttttaa aatctatttc tttaacgtta aagatattta ctaaacacgc
    55201 tcgactaatt cttgttaaca taggatattt cttttcaagc tcactaattc ttccataaaa
    55261 ttcgtactgc ccatctttag tacacaatga taatctatgt ggtaattttg aagcttctac
    55321 aaaataaatg tcatttatat caaaagtaaa tgtttgagaa cctatggaaa aaacaaatgc
    55381 ttgattcgta acctgttttg ttgcatctat cctttcttgc gctaataaca ataactcaac
    55441 aatctcgttt cgatactcat ctagtgtttg atcttttgtt acaaatccta acgtttcaac
    55501 tcttcttttt attgttacgg gtaacatctc atcatgagta gttgtaaaaa tgatttttgc
    55561 ttgaacatca tattttctga ttacttctgc taattctata ccgttaactt catgatttaa
    55621 atcaatatct aagaaatata ttccttgctt tggtcggaat tgtttgaggt attttttaac
    55681 ttctaacgga ctttgtgttt tcaatactat cttaaa
//
LOCUS       DACEPD010000009        53491 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.009, whole genome shotgun sequence.
ACCESSION   DACEPD010000009 DACEPD010000000
VERSION     DACEPD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.009"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..340
                     /locus_tag="IW797_000495"
     CDS             <1..340
                     /locus_tag="IW797_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ1223292.1"
                     /translation="LVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELSRKSVQTL
                     FKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWILLKQGGL
                     ALLALLSIFL"
     gene            463..2094
                     /locus_tag="IW797_000496"
     CDS             463..2094
                     /locus_tag="IW797_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ1223293.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(2156..2797)
                     /locus_tag="IW797_000497"
     CDS             complement(2156..2797)
                     /locus_tag="IW797_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1223294.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            3079..3387
                     /gene="rplU"
                     /locus_tag="IW797_000498"
     CDS             3079..3387
                     /gene="rplU"
                     /locus_tag="IW797_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ1223295.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            3414..3758
                     /locus_tag="IW797_000499"
     CDS             3414..3758
                     /locus_tag="IW797_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ1223296.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3771..4064
                     /gene="rpmA"
                     /locus_tag="IW797_000500"
     CDS             3771..4064
                     /gene="rpmA"
                     /locus_tag="IW797_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ1223297.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4471..7305
                     /locus_tag="IW797_000501"
     CDS             4471..7305
                     /locus_tag="IW797_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1223298.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            7496..7987
                     /locus_tag="IW797_000502"
     CDS             7496..7987
                     /locus_tag="IW797_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ1223299.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            8090..9079
                     /gene="manX"
                     /locus_tag="IW797_000503"
     CDS             8090..9079
                     /gene="manX"
                     /locus_tag="IW797_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ1223300.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            9112..9915
                     /locus_tag="IW797_000504"
     CDS             9112..9915
                     /locus_tag="IW797_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ1223301.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9936..10850
                     /locus_tag="IW797_000505"
     CDS             9936..10850
                     /locus_tag="IW797_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ1223302.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10933..11307
                     /locus_tag="IW797_000506"
     CDS             10933..11307
                     /locus_tag="IW797_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ1223303.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLTGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11446..11691
                     /locus_tag="IW797_000507"
     CDS             11446..11691
                     /locus_tag="IW797_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1223304.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11863..12927
                     /locus_tag="IW797_000508"
     CDS             11863..12927
                     /locus_tag="IW797_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1223305.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            13173..13598
                     /locus_tag="IW797_000509"
     CDS             13173..13598
                     /locus_tag="IW797_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1223306.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13591..14040
                     /gene="nusB"
                     /locus_tag="IW797_000510"
     CDS             13591..14040
                     /gene="nusB"
                     /locus_tag="IW797_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ1223307.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            14137..14991
                     /locus_tag="IW797_000511"
     CDS             14137..14991
                     /locus_tag="IW797_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ1223308.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14992..16329
                     /locus_tag="IW797_000512"
     CDS             14992..16329
                     /locus_tag="IW797_000512"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ1223309.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            16334..16558
                     /locus_tag="IW797_000513"
     CDS             16334..16558
                     /locus_tag="IW797_000513"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ1223310.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16560..17450
                     /locus_tag="IW797_000514"
     CDS             16560..17450
                     /locus_tag="IW797_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1223311.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17459..18277
                     /locus_tag="IW797_000515"
     CDS             17459..18277
                     /locus_tag="IW797_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ1223312.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            18384..18833
                     /locus_tag="IW797_000516"
     CDS             18384..18833
                     /locus_tag="IW797_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1223313.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18851..20533
                     /gene="recN"
                     /locus_tag="IW797_000517"
     CDS             18851..20533
                     /gene="recN"
                     /locus_tag="IW797_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ1223314.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20588..20704)
                     /locus_tag="IW797_000518"
     CDS             complement(20588..20704)
                     /locus_tag="IW797_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ1223315.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(20777..21733)
                     /locus_tag="IW797_000519"
     CDS             complement(20777..21733)
                     /locus_tag="IW797_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1223316.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            21904..22272
                     /locus_tag="IW797_000520"
     CDS             21904..22272
                     /locus_tag="IW797_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ1223317.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22442..22873
                     /gene="mraZ"
                     /locus_tag="IW797_000521"
     CDS             22442..22873
                     /gene="mraZ"
                     /locus_tag="IW797_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ1223318.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            22894..23853
                     /gene="rsmH"
                     /locus_tag="IW797_000522"
     CDS             22894..23853
                     /gene="rsmH"
                     /locus_tag="IW797_000522"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ1223319.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            23858..24259
                     /gene="ftsL"
                     /locus_tag="IW797_000523"
     CDS             23858..24259
                     /gene="ftsL"
                     /locus_tag="IW797_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ1223320.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            24260..26452
                     /locus_tag="IW797_000524"
     CDS             24260..26452
                     /locus_tag="IW797_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1223321.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26478..27440
                     /locus_tag="IW797_000525"
     CDS             26478..27440
                     /locus_tag="IW797_000525"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ1223322.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27454..28815
                     /locus_tag="IW797_000526"
     CDS             27454..28815
                     /locus_tag="IW797_000526"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ1223323.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            28841..29929
                     /gene="murG"
                     /locus_tag="IW797_000527"
     CDS             28841..29929
                     /gene="murG"
                     /locus_tag="IW797_000527"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ1223324.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            29944..31164
                     /locus_tag="IW797_000528"
     CDS             29944..31164
                     /locus_tag="IW797_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ1223325.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            31320..32645
                     /gene="ftsA"
                     /locus_tag="IW797_000529"
     CDS             31320..32645
                     /gene="ftsA"
                     /locus_tag="IW797_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ1223326.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32668..33909
                     /gene="ftsZ"
                     /locus_tag="IW797_000530"
     CDS             32668..33909
                     /gene="ftsZ"
                     /locus_tag="IW797_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ1223327.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            33926..34603
                     /locus_tag="IW797_000531"
     CDS             33926..34603
                     /locus_tag="IW797_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ1223328.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34617..35216
                     /locus_tag="IW797_000532"
     CDS             34617..35216
                     /locus_tag="IW797_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ1223329.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            35231..35518
                     /locus_tag="IW797_000533"
     CDS             35231..35518
                     /locus_tag="IW797_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ1223330.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35567..36349
                     /locus_tag="IW797_000534"
     CDS             35567..36349
                     /locus_tag="IW797_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ1223331.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            36370..37077
                     /locus_tag="IW797_000535"
     CDS             36370..37077
                     /locus_tag="IW797_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ1223332.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            37339..40137
                     /gene="ileS"
                     /locus_tag="IW797_000536"
     CDS             37339..40137
                     /gene="ileS"
                     /locus_tag="IW797_000536"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ1223333.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(40186..41712)
                     /locus_tag="IW797_000537"
     CDS             complement(40186..41712)
                     /locus_tag="IW797_000537"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ1223334.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(41727..42374)
                     /locus_tag="IW797_000538"
     CDS             complement(41727..42374)
                     /locus_tag="IW797_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ1223335.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42558..42887)
                     /locus_tag="IW797_000539"
     CDS             complement(42558..42887)
                     /locus_tag="IW797_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ1223336.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            43064..43792
                     /gene="sfsA"
                     /locus_tag="IW797_000540"
     CDS             43064..43792
                     /gene="sfsA"
                     /locus_tag="IW797_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ1223337.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            43808..44821
                     /locus_tag="IW797_000541"
     CDS             43808..44821
                     /locus_tag="IW797_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1223338.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALIDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            44821..46098
                     /locus_tag="IW797_000542"
     CDS             44821..46098
                     /locus_tag="IW797_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1223339.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            46161..48863
                     /locus_tag="IW797_000543"
     CDS             46161..48863
                     /locus_tag="IW797_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1223340.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            49015..49332
                     /locus_tag="IW797_000544"
     CDS             49015..49332
                     /locus_tag="IW797_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1223341.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            49362..49682
                     /locus_tag="IW797_000545"
     CDS             49362..49682
                     /locus_tag="IW797_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1223342.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            49790..51250
                     /locus_tag="IW797_000546"
     CDS             49790..51250
                     /locus_tag="IW797_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1223343.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            51318..51539
                     /locus_tag="IW797_000547"
     CDS             51318..51539
                     /locus_tag="IW797_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223344.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51570..51752
                     /locus_tag="IW797_000548"
     CDS             51570..51752
                     /locus_tag="IW797_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ1223345.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            51752..52165
                     /locus_tag="IW797_000549"
     CDS             51752..52165
                     /locus_tag="IW797_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ1223346.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            52288..53469
                     /locus_tag="IW797_000550"
     CDS             52288..53469
                     /locus_tag="IW797_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1223347.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
BASE COUNT        17702 a         9061 c        11587 g        15141 t
ORIGIN      
        1 tttagtgact ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac
       61 tgattctgac acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc
      121 tgttcaaaca ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact
      181 ttatggagct tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat
      241 tttttttgtt gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg
      301 tgggctagct ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt
      361 tatctaacag gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat
      421 gaatggtatt ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt
      481 tcctcactaa aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg
      541 gctgggatca cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc
      601 ggagctgatg cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt
      661 ttatcaggcg gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg
      721 tctattgcgg caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta
      781 tttttacttg cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc
      841 gtaattacgg ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat
      901 ttatttggtg ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg
      961 ataggatttc cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg
     1021 tttatctatc caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg
     1081 gcaacagcag ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa
     1141 acattgatca gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa
     1201 gtggcagttc cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt
     1261 gcttcggctg gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa
     1321 ggaattggaa atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc
     1381 cgtacaagcg tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca
     1441 gttgtcttac tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca
     1501 gcacttgcag gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag
     1561 gagctattta ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt
     1621 acagttatct ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt
     1681 tttgttgtca aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg
     1741 gggctgccag aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg
     1801 ttttttatgt cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt
     1861 gtcattttct ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat
     1921 gaatttcaag aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca
     1981 gaagtagaaa aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca
     2041 gttgctcatg cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat
     2101 gaaaaagaac agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat
     2161 attgctgtaa aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc
     2221 gattgtagaa aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc
     2281 attcgtaggc atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat
     2341 gatttttggt aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg
     2401 tcgcaattaa ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa
     2461 atacttcgga acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt
     2521 tattgatccg atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct
     2581 cttctcgtgt taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc
     2641 taaaaggaac atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt
     2701 taggatgaag aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta
     2761 aagaagcttc ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta
     2821 ttataagaat aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa
     2881 aagcagaagt attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac
     2941 ggttgctgtt ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc
     3001 agaacaagta ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag
     3061 aaggaggtgc tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt
     3121 gaagtgggac aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt
     3181 tttgacgaag ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca
     3241 ggtgcaactg ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc
     3301 aaatacaaac ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa
     3361 gtggttatcg acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca
     3421 aaggaacatt taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg
     3481 ctgatgctgg tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca
     3541 gcactgtcaa cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag
     3601 aagaaggcgg ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata
     3661 ttgctgagat tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact
     3721 cagaatatat ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat
     3781 ctatgaatct acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg
     3841 actcagaatc aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat
     3901 caattcttta ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg
     3961 gagacgacac tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta
     4021 acaaaaaaca agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta
     4081 aagaggttgt gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag
     4141 tggaggctac gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat
     4201 caggatgttt tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg
     4261 aaataattat atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg
     4321 atttttcgtt cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa
     4381 tcaggtaaat agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt
     4441 acaatagtct atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg
     4501 tatgtcaaag aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca
     4561 caggaaattg ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag
     4621 ctagtgcgcg aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa
     4681 acactcgtta cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg
     4741 gtctctgaag aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt
     4801 ggagctaacg gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg
     4861 ccaagaggat taaattgttt gattacagga cctaccggat caggaaagac ttattttgca
     4921 cacgcgatgt atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt
     4981 gttttcaatt gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg
     5041 tacgcaaaag gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg
     5101 gatggcggta tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg
     5161 atcttttact ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat
     5221 gaagcaaatg tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa
     5281 acttttgttc ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca
     5341 aatgagaaag tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga
     5401 aagatttctt tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat
     5461 ataggacaat tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg
     5521 caatcacccg aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg
     5581 attcaattag caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag
     5641 ataactgtag caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct
     5701 tataatctat atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat
     5761 caagaagcaa tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat
     5821 aaagaccatg gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa
     5881 atcatagaag tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt
     5941 cagcaaaact ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac
     6001 ttaggagaag aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat
     6061 gaatatgctg ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt
     6121 ccagaaagtg aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca
     6181 agcggcagaa tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc
     6241 caagttgtcc aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat
     6301 atggatccga aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga
     6361 agcggggtca tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa
     6421 agagacactg gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa
     6481 actgtacgaa aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa
     6541 aattttcgag gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt
     6601 cataagaaaa aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc
     6661 aaagaattga tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct
     6721 ttgtcgattg tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata
     6781 gctactactg ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt
     6841 attgatcaaa atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa
     6901 actgaattca tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct
     6961 gataacttca tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca
     7021 caaatctctc agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg
     7081 atgcacacag caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa
     7141 aacgaattga caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt
     7201 ctgttggaag atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt
     7261 ctagtccata atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat
     7321 acacttttat agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg
     7381 tatcgtaaaa aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt
     7441 cacacagaaa tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga
     7501 tattcgattg gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc
     7561 gaagcaaaca ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt
     7621 gagaaagttt ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt
     7681 gcagaaaatg attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt
     7741 attcaccaat ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt
     7801 gaatatcggc ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt
     7861 aaatcaacaa gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat
     7921 tcgaaaagta cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa
     7981 ggtgtagcaa gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt
     8041 attaatattt ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat
     8101 tatcctagca agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt
     8161 tggcgaacaa gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt
     8221 gagagccaaa ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt
     8281 agttgattta tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa
     8341 agataaatgg gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc
     8401 acgcttttca atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga
     8461 cggtgtaaaa gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga
     8521 agacgcacaa cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa
     8581 atatgtatta gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac
     8641 aaaagcagtg caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct
     8701 gcgtaagaga ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat
     8761 tagcaaaatg atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt
     8821 atttgaaaat cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt
     8881 aaatgttggg tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat
     8941 gggacaagaa gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt
     9001 acgtaaagta cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc
     9061 tgaattagca aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt
     9121 atttcaataa ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat
     9181 gaatttcaat ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac
     9241 ctcgaagctg gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat
     9301 atcggtgccg ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta
     9361 gttttaggtg gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg
     9421 gctgttgcag ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat
     9481 ttgatggatg cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt
     9541 gctgtatgtt tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca
     9601 gcacaaactg tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct
     9661 atcggtgggg gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca
     9721 aaagaagtat ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca
     9781 ttgattgctc taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa
     9841 atgggtggtt catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt
     9901 ttgaatgact attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat
     9961 caaaaaaaga tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact
    10021 acgaacgtat gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat
    10081 acaaaacaaa agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac
    10141 acccatacat tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga
    10201 atggcgcgcc tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt
    10261 tagctggtgt tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag
    10321 gtgcttcatt agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga
    10381 acattattcg ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta
    10441 aaattactga tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt
    10501 tagggatgtt cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag
    10561 ttgtatcaac tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag
    10621 gtggtgaagg aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg
    10681 aaaaggtaac aacattacaa gacaacttag accaattgat tcctggttta gctgcattag
    10741 gattaacatt gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc
    10801 ttggtttatt cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa
    10861 tatagctcgg gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc
    10921 tggagggaga aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact
    10981 tcgtttacgg gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt
    11041 taccatgcgc gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta
    11101 ctagcatcag tcatgctaac gggaaaatgg attcctcgat atgccattca aacgaaaaaa
    11161 aatggtacat acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa
    11221 tatgtagatg aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt
    11281 attcgtagta tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct
    11341 ttattttttt actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt
    11401 aaatcccata tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg
    11461 acattgattg ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat
    11521 tcgatgggct gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg
    11581 attttcggcg aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc
    11641 gcagtgattt tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca
    11701 aaatagtaaa ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt
    11761 aaaataaaaa gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg
    11821 ctataatgga aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa
    11881 aaattgcgca aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac
    11941 aatcttcgtt acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa
    12001 aaagcatttt ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg
    12061 tttgaaatta ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa
    12121 gaagaactgc actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt
    12181 ttagaagaaa tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga
    12241 gaagtgaaag atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg
    12301 gcatatgatc acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat
    12361 caactagact tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt
    12421 gctagcggat tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa
    12481 ggtgacttga tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca
    12541 cgtacattcg ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta
    12601 gatgctcaat tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat
    12661 gcagttgcta gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca
    12721 ggacatggga tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag
    12781 caatttgtac caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt
    12841 ggagtacgta ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt
    12901 tcaccaaaag aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca
    12961 gtgttcacag actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt
    13021 ttaagctgta agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg
    13081 atcggcggca gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta
    13141 tctcgtgctt aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa
    13201 acgcaaacca agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg
    13261 ggattgcagc ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg
    13321 tcactgaatt attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag
    13381 atggcttgaa agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag
    13441 caatggaaat gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat
    13501 ctgaagtcaa tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg
    13561 aagaactttt tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt
    13621 cgggaaatgg cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa
    13681 gatgcgattt tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca
    13741 gagtttgttc ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa
    13801 ttggataaag tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa
    13861 atggatttgg tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca
    13921 gcggctgttg ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca
    13981 cgtaaattcg tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa
    14041 gtcctagctt ttttctatga atcatcataa acctgttata aatcgggaaa attctatggt
    14101 agaatgaaaa cacaaaaaat atctgggagt ggttagatgg cagaattgat taacggaaaa
    14161 gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat
    14221 ggtatccatc ccggattagt cgttttattg gtaggggaaa atccagccag ccaaatctat
    14281 gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc attcattggt agagcgttat
    14341 cctgagacga tttctgaaga cgaactactt gcagaaattg aaaaatacaa tcaagatcct
    14401 agttttcatg gtatattggt ccaattacca ttgccaaagc atatagatga agaaaagatc
    14461 ctgttagcta tccgccctga aaaagacgta gacggttttc atccgatgaa tttaggcaga
    14521 ctccttgcgg gtaaaccgga caagattcct tgtactccat acggcatcat gaaaatgttt
    14581 gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc
    14641 gtcggtaaac cgatggctca gctactcttg atggcagatg caactgttac tgttgctcat
    14701 tctaaaaccg aaaatctggc tgaactggca caagaagcag atattttagt cgtagcaata
    14761 ggacgtggac attttgtcac aaaagaattc gtaaaaccag gtgctgtagt gatagatgta
    14821 gggatgaatc gtgatgctaa cggtaagctg atcggtgatg tgaaatttga tgaagtggag
    14881 cctattgcag gctggatcac tcctgtgcca aagggtgtag ggccaatgac tatcacgatg
    14941 ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag caggtgaata gatggcacaa
    15001 gaatatttaa ctgtcactac attaacgaag tatttgaaaa gaaaatttga tgccgatcct
    15061 tatttagaac gcgtatattt aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc
    15121 catcaatatt tcagcttaaa agatgaccat gcaaaaatat ctgctattat gttcaaagga
    15181 gcattccaaa aattacgatt ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt
    15241 atttcattat atgaagcggg aggttcttat caaatctatg tagagcacat ggagcctgat
    15301 ggaataggtg ctttatatca agaattggaa gaacgaaaag aaaagctgat gaaggaaggc
    15361 ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc
    15421 agtccaagcg gagcggttat ccgcgatatc atcacgacgg tgaaaagaag atatccaatt
    15481 gtccaattgg ttttgttccc aacagttgta caaggaaacc aagcagcgga caatgttgtc
    15541 aggaatatcc agagagtaga agagatgggc aatttcgata caatgatcat cggtcgtggg
    15601 ggtggttcga ttgaggattt atggccgttt aatgaagagc gggttgctcg ggcgatttat
    15661 caagctaata cgccggtgat ttcatctgta ggacatgaaa cagataccac gatcgctgat
    15721 ttagtagcag atgttcgggc tgccactcca acagcagctg cagaattagc tgttcctgtt
    15781 ttgtcagaag agttgatgcg cattcaagaa cggcaagcta gattgcagca ggcgtttgta
    15841 cgccagatcc agcgcaaaca agaacggttc gagcgtgctc gacgttccta tgtatttcgg
    15901 cagcctgaaa gattgtatga aggtcagacc atcaaattag accagttgaa acagcgatta
    15961 tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc attaagccac
    16021 caattggaac aagtggctcc gctttatcga gttaaagcag ctcgccaaga aactgcttat
    16081 ctagaaaaac gcttagaaga gaaaatccaa caatatatga aacaacagca gcagcgtttt
    16141 gctcaagctg ttcaatctct tgatttgttg agtccattga aaatcatggg aagaggctac
    16201 agctatacga cgttaaatga tcaagtagta aaaagtgtca ccgagattca tcaaggggat
    16261 ttgctgacga tccattatca agatggaatc gttaaaggac aagtacaaga gatagacaaa
    16321 ggagaataaa aatatggaaa atccaacttt tgaagagtca ctgcaagaat tagaaaaaat
    16381 tgtcatgcag ttagaacaag gagacgttcc attagaatca gctctagatt cgtttaaacg
    16441 aggaatggaa ttaagcaaac attgccaaga tacattagct aaagcagaag agaccttgac
    16501 gaaaatgatg acagaatcaa atgaagaagt tgattttgac ggaaacgagg aaagctgaga
    16561 tgagattaac tgacttttca gctattcatc tacctgctgt agaaaaagaa atcttgtctt
    16621 ttttagatga gcatacaacg gatagaggac tctatgatgc gatgagttat tcagtgaaag
    16681 caggcgggaa aagaatacgt cccttattac ttttaacggc cgttgcttcc tttgatgagc
    16741 ctattgatgt tcccgtctat caagtagcag ctgctttaga aatggtgcat acgtattcct
    16801 tgatccacga tgatttgcct gcgatggaca atgatgatct tcgtagagga aaacctacca
    16861 atcataaagt attcggcgaa gcattagcta tattggccgg agatggtttg cttacaggag
    16921 cattccagct aatcagtatg gcacacttag gtaattctcc taagcttctg ctgcttcagc
    16981 aattagctgt ttgtgcggga tcacaaggta tggttgctgg gcaagctgca gatatcgagg
    17041 gcgaatcgaa aaagctttct ttagaagaat tagcatttat tcatgaaaga aagacagggc
    17101 atttgattcg ttatgccttg ctggctggtg gcatattagc gaagcaacct gaagaaatct
    17161 tattgctact tcaacgatta gccgaacatt taggtttagc ttttcagata agagatgatc
    17221 tactcgatgt gattggtaca acgaaaacat tagggaaaac agctggtaaa gatgaacgta
    17281 tggaaaaaaa tacttacccg cgtttattag gcctagaaaa gacacgtgag gcgttggaga
    17341 ttgagctgtt atccgccaac aagatcatag acaaattaga agaggacgtt ttttcttttg
    17401 atggcgggct gatgcgccag atgatcaagc aatttgatgt ggagaaatga gggaaagaat
    17461 ggaaaaagaa cgcgtagatg tattagcggt caaacaagga ttatttgaaa caagagaaca
    17521 agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa
    17581 accgggagaa aaaattccag tgagtagtga attaagagtc aaaggtaaaa aacttcctta
    17641 tgtttctcga gggggattaa aattagaaaa agcactcaaa gcatttgatc tatctgttga
    17701 aggaaaaaca ttgttagata ttggtgcatc cactggcgga tttactgatg cagctttgca
    17761 aaatggcgca aaaatgagtt atgctttaga cgttggctac aatcagctag cttggaaaat
    17821 aagacaagat cctagagtcg tcgtaatgga aagagtgaat tttcgttatg caaaacccga
    17881 agactttacc ttaggaattc cagaagttgc tgttatcgat gtttctttta tttcactaaa
    17941 actgatgctg cctccattac atgctatttt aaaagaagag ggagaagtga ttgctttaat
    18001 caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc
    18061 tcagacccat caaaaagttt tagaagacat tttgtcattt gcggcacaag gtggatatga
    18121 tgtgctggaa ttaagttatt ctccgatcac ggggggagaa ggaaatatcg agttcttggc
    18181 tcacttgagg aaagtacccg aaagcggaac aatcaattca gctattaata tggcagaagt
    18241 ggtttcaaat gcacacgaac agtttgacca taaataaatt gtaactgtga aaatcaagtg
    18301 agaagtatgc ggattcttct ttattatacc aaggaaataa gctatcattg attaagaaga
    18361 atatgctaag gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg attactcgat
    18421 tgttgagtga gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag
    18481 gaatatctgt tactcaagct acgatttcca gagatataaa ggaactgaaa ctgatcaagg
    18541 taccggcttc agatggcggc taccgttata gcataccagc tgaaacaaac gaagatgtaa
    18601 atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat
    18661 ttgttatttt gaaaacattg ccgggaaatg cttcggcagc agcaaatttg attgataaac
    18721 gttacaagaa agaattattt tcgattatca atgatgacga taatgttttg atgattacac
    18781 gaactgaaga ccaggcagtt ttattgaaga aagacttcct gcattacctt taagttgagg
    18841 agcgaatgtg atgctacaag aaatcagtat tactaacttt gcgattattc cggaattgcg
    18901 gctttctttt cacgaaggaa tgaccgcgct gaccggagag actggtgcgg gtaaatccat
    18961 tattatcgat gctttaggtc tgttagcagg aggaagaggt tcaagcgatt atatacgtca
    19021 aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag aaggattctc
    19081 tgaactgatg gttgaattag ggatcgaaac agatgaggac aatctgattg ttcgacggga
    19141 tatgtcctta actggaaaaa atgtttgtcg agtgaatggt catattatca cattagctaa
    19201 tctgagaaga atcggcagtt atttagtaga tatccaagga caaaacgaac atcaggaact
    19261 gctccagcct gaatcacatc ttgctttatt ggatcgtttt ggagatgtag ctttccaaca
    19321 gaagaaaaag agttatcagc aagaatatat ctcttatcgt gagctggaaa aaagggtacg
    19381 aaaagtgcag caaaatgaaa aatcctatgt tcagagaata gatatgctcc atttccagca
    19441 agaagaaata gctgctgctg atttgcaagt cggagaagaa gagaaattaa aagaagaaag
    19501 agaaaaactt agcaattatc aaaaaattgc tgatggatta gctgcaggtt acggagcact
    19561 gacgaataat gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag aactgcaagg
    19621 gatcgcccat ctagatgtgg agtatgaagc aatttatgaa aatatccaaa gtgcttacta
    19681 tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt gatttattgg agttagatga
    19741 gagtcgttta gaagaagtta cgcaacgttt agaattaatc cgacagttga aacgtaaata
    19801 cggcgaatcc attgagtcaa tattggctta ctatgacgaa atcaccgaag agctagcttc
    19861 ttctgatttt tcagaaggtc aattggacaa aatgaaagag gaactagagc aaaaagagct
    19921 tctacttcag caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt
    19981 ggaaaaatcc attctgcatg aattgaaaag tctgtatatg gaaaacactg agtttgaggt
    20041 acgtttctta aaagaagaaa atagacaatt gaatcatgat ggatttgacc aaatcgaatt
    20101 ttacattacg accaatccag gagagccact gaagcctttg gtgaaagtcg cttctggtgg
    20161 cgaactgtct cggatgttgc ttgctttgaa aactattttt tcttctgagc aaggtgtgac
    20221 aagtattatt tttgatgaag tggatacagg tgtgagcgga agagttgcac aggccatcgc
    20281 agataaaatt tctcaaatat cgaaatactc tcaagttttg tgcatcactc atctgcctca
    20341 agttgctgca gtcgccgact atcaatatta tattgtgaaa gcagtgattg atggacgtac
    20401 gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct
    20461 ggctggaagt gagattacaa aactcacact cgaacatgca aaagaactat tgaaactcgc
    20521 aaaaaggacc tgagccacct cgcagtggtt caggtccttt tcagcagctg atagctatat
    20581 tttattttta catgacactc attacagcag tcaatacaac agatccaatc gttaaaatca
    20641 gcattgtcca aatgacaaat tttgttactt tagcaaaagt acttgtttgt tttttttcac
    20701 gcattgtttt caacctcgtc attaattagt tgattcaagt gtacaatctt ttttccattt
    20761 ccacaagagg gcttttttat aaaaacttcc ctttttttaa ataataggta gttacgatac
    20821 aaataaataa tgttaataga actagccata taaaggcatc atccctttca gcaataggta
    20881 acttaatgtt cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta
    20941 aagaagttaa aattttcata atattattca gattattcga tacgattgct gaaaaagtct
    21001 gattcatttg ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt
    21061 ccaccaatac atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt
    21121 gcgctagttc tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc
    21181 ttttttggat atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg
    21241 cttccaaagt atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga
    21301 cctgccaaat gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg
    21361 tctggctaaa gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct
    21421 ttttgggcgt caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac
    21481 ttgttttagc aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat
    21541 tgaattcttc tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg
    21601 gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat
    21661 accagatcgt ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa
    21721 aatatttgat catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat
    21781 tggaatacat ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat
    21841 gaaacagttg tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg
    21901 ttcatgggca cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg
    21961 tttggttgcc aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc
    22021 cctgatttgg cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg
    22081 acaatttcta tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct
    22141 gttttccaag catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg
    22201 cgtttggttt ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt
    22261 tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct
    22321 tgatttttaa aaaaatgttt atgaataaaa aaagttgttc ccggatttcc cagaaaaggt
    22381 ggtagaaagt gggggattgt ggtagactaa ttttagttag tggaggaatg gggggctttc
    22441 aatgttgatg ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc
    22501 aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg atggctgtct
    22561 tttcggctat ccgatgagcg aatgggaaaa tctggaagct aaactgaacg aaatgccgtt
    22621 ggctaaaaaa gacgcacgta cttttgtgcg attcttctat tcggctgcca ctgaatgcga
    22681 acttgataaa caaggacgaa tcaatatacc cagcacgtta agaaattatg cagctttgac
    22741 aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg agacacgatg
    22801 gcaagatttt tctgcagcag cagaagagaa ctttgacgaa attgctgaaa gtatgattga
    22861 tttcggacta taagaatagg aagaggctga ctaatgactg aaacatttca acactataca
    22921 gtcatgttga aagaaacggt agatggattg aacatcaaac cggatggcgt ttatgtagac
    22981 tgtacactcg gcggtgccgg acatagtgaa tatttattaa gccaactaag ttctaaaggg
    23041 catctgtatg cgtttgacca agatcaaaaa gcaatcgatc acgccaagct gcgtttagct
    23101 tcttatgtcg aacaaggtat ggtcacattt atcaaagcga acttcagaga attagaagaa
    23161 cgcttgagtg agcatgtaga taaggtagac ggcattttgt atgatctagg ggtttcctct
    23221 cctcagctag atgaagcaga acgtggattt agttatcatc aagatgcacc gttggatatg
    23281 cgcatggatc agtcagctcc tttatccgct tatcacgtaa ttaatgaata ttcctaccat
    23341 gaattagtaa agattttctt ccgctatggc gaggagaaat tttccaaaca aattgctcgt
    23401 gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc
    23461 attaaacaag tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt
    23521 tttcaagctg tgcggattgc agtaaatgac gaactcggtg ccgaagaggc ctctttagaa
    23581 caggcaatcc ggttattgaa agtcaatgga agaatcagcg taatcacatt ccattcattg
    23641 gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga tctaccaccg
    23701 ggacttcctg tcatacctga cgaattccaa ccagaactga aagtcattac tcgtaaacca
    23761 atccttcctt cagaggaaga gctaacagaa aacaatcgat cacgcagtgc aaagttacgt
    23821 attgcagaaa aaataagaat gaaagaggag tagaatgatg gcagaattaa agaaaatgca
    23881 agattacgaa tatgatttac ctgaattggt ggatcagccg gaacaacaaa tgcctgattc
    23941 ttcttcgaat caagaaatca ttcttccaca atcaccggca aaacgattaa agcagatttc
    24001 tgctttggaa aaaattgctg tggcaagcgt ggcgattgct attgttgctt tatgtgtttt
    24061 aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt caatacaaac
    24121 agaaatcaat gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa acgaactttc
    24181 tcgtactgaa cggattaaac aaatcgcaga agaaaaagga ttgtctatta atgacgacaa
    24241 tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta tggaaaagaa
    24301 aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc attttatttg ctacgagtat
    24361 tggattgttc tttttatttg ccgtccgttt ctcgtatatc gttattggag gtcatgttgc
    24421 tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc
    24481 aaaaagagga acgatttatg atcgtaacgg tgtggcgctc gcagaagatg cctcctctta
    24541 ttcaattaaa gcgatactct caaaaacata tacctctggg gataaaaaac tgtatgttga
    24601 agaaaagaat tttgataaaa tagcagaaat cctgcataag aatctttcta tagacaaaaa
    24661 agatgcactg aatattttag aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg
    24721 tagctatggg aaaaatatct cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa
    24781 agaaggtgtc gctggtcttt attttgtcga ccatcaggct cggatgtatc caaatggcgt
    24841 cttttcttct cattttattg gatacgcagt tccagacaaa gatgagaatg gcttagttgg
    24901 taaactaggt ttggagtctg cttacaatga tatcttgagc ggtaaagatg gtaagattat
    24961 ttatcaaaaa gataattttc aaaatcctct gccaggtaca gtggcagaag aagaaaaagc
    25021 tgtcgacgga caagatatct acacgacttt agacagcaga ctacaaagtt atttagaaac
    25081 actgatggat caagtaaatg aagaatatca gccagaagaa ttgacagcag tcctgatgaa
    25141 agcaaaaacc ggagaaatct tggcaatggg acaacgacct actttcaatc ctgaaacaat
    25201 ggaaggattg actggaaaag atgctatctg gcgtaatttc ttggtacaag atagttatga
    25261 accaggatca accatgaaag ttttcactac tgcagctgct atcgaagaag gagaattcaa
    25321 cgagaatgaa accttccaat ctggaaaaat ccaagtagaa gatgctacga tcaatgacca
    25381 tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt caagtaacgt
    25441 cgggatggtc atactggaac agcgattagg cggacgatgg tataactatt tacaaaaact
    25501 gggatttgga caaagtactc attctggatt agatgatgaa gtaaacggtg ctttacctac
    25561 attaaatatt gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg tgacgaattt
    25621 ccaaatgatg aaagctttca cttctattgc gaataacggt acaatgattc aacctcgata
    25681 tatcagcaaa gtggtggatc cccaaactgg agaagagcga acaactcaaa cagaagttct
    25741 agggcaacct ttttcaaaag aaacaacaga aaaagtccgt gaatatatga gagatgttgt
    25801 cgaaagcgaa aactacggta gtgcttatgg tgtatatagt gtacctggat acaatgtatc
    25861 ggcaaaaaac ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa caggagatac
    25921 agcctacctg tattcgattg tggagatggt tccttctgaa gatccagact atgtcttgta
    25981 tctaacaatg aaacacccta agacatatga tcgaatggca ttggctaaaa tcgcaaatcc
    26041 attgatgaaa cgggcaatgg attttaaaga aactgaagaa gattcagata cagaaacaaa
    26101 aacagaaaaa gtatccgtag ccgattatcg taatcttgaa gcggacgttg cagcagctga
    26161 tgctcaaaaa agtggactgc aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca
    26221 atctactgcc aacggtgatc agttgatctc tggagaaaaa ctgatcctat atactggtgg
    26281 tgataagctg atgcctgacg taacaggatg gtcaaaagca gatatcatga agctggggaa
    26341 aatcctcggt attgaagtta gttttgatgg agatggttat tgtgtcaaac aagaacttgc
    26401 tccatatgaa aaaatcactg aagacaaact tagttttact ttagaagaat aagaagagaa
    26461 ataggagaga aaagagcatg gaatggacac aagcattgat tcccattgtc agcagttgtg
    26521 caatgacaat tgccgcaatg ccgctattta ttggttattt tcaaatgaaa aagcaaggtc
    26581 aggcgattcg tgaggaggga ccaaagtggc acaatagcaa agccggaact cctacaatgg
    26641 gcggcttagt gtttctgatc ggctctattt tgacaggaat ttgggttgga gcttggcaaa
    26701 aacagctaac tcctactctt ttcatcttgc tttttgtatt agcgttatac ggagtaatcg
    26761 gctttcttga tgattttata aaaatattca agaaaagaaa catgggactg aactcgaagc
    26821 agaaattgat tggacagatt ctagggggaa tcatctttta tcttgtttac cgttctgaag
    26881 gttatccagg tgtattgaat ttcttcggac tagatctacc tttaggaatc atttatggta
    26941 ttttcgctct cttctggctt gttggatttt caaatgcagt aaatctgaca gatggcatcg
    27001 atggattagt tgctggctta ggaagtatct cttttgcagc ttacggtcta attgcttggc
    27061 atcagcatca gtatgatgta ttaatcattt gcttaagtgt tttaggaggt ttactgggtt
    27121 tcttcgtcta caacagaaaa cctgccaaga tttttatggg agatgtcggt tcattagcgt
    27181 tgggcggact tttagcggca atttctataa tgctaaacca agaatggaca ttgcttttga
    27241 ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct tcaagttact tcctttaagc
    27301 ttacaggaaa acgaattttt aaaatgtctc caattcatca tcactttgaa atgtgcggat
    27361 ggtcggagtg gaagattgat acagtttttt ggttgatcag cattgtgact tcgttgatca
    27421 cattatggat cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat
    27481 aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa attattacat
    27541 gaattgggcg cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca
    27601 caagagctgc tttctttagg gataaaagta ataacaggta gtcatccaat cgaattgttg
    27661 gatgaagaat tttcattgat ggtcaaaaat ccagggattc cctacagtca tcctttagta
    27721 gcaaaagccc aagaaatggg tattccggta attacggaag ttgagctagc ttacgaagta
    27781 gcggaatgcc caattattgg gattacagga acgaacggaa aaacaacgac aacaacgatg
    27841 acaggactac tactgaatgc aggagcagat caaggaattg cacgtttagc gggtaatatt
    27901 gggtatccag caagtggtgt agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa
    27961 ttgtctagct tccaactcat gggaatcaca gatttccgcc ctcatatagc ggtgattacc
    28021 aatatctatg aagcacatat tgattatcat ggtacaagaa aagaatatgt caaagctaaa
    28081 tggaaccttc aaaaaaacat gacagaaaaa gattatctga tccttaactg gaatcagtca
    28141 gaactacaag aattagcgca aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa
    28201 gtgttggaag acggtgtgta tgctgacgat tactcaatat actataaaaa agaaaaaata
    28261 atggaaatat ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc actagctgct
    28321 atttctgtag caaaattgta tggtatatca aatgaagcta tacgagaaac gttgcatttt
    28381 ttccatggcg taccacatcg tacgcaatat gtaggagaaa tccaaggcag aaaattttat
    28441 aatgattcaa aagcaaccaa tattttagcg actaaaatgg ctctaagtgg atttgaaacg
    28501 tctaaggtcg tgttattggc tggcggatta gacagaggaa atacatttga tgaattaatt
    28561 ccttcattaa aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga aaagttgatg
    28621 gatgctggaa aaaaagcagg catcgagaca atagtaacag ccgattctgt agaacaagca
    28681 gttcctttag cattagaaaa cagtacagat ggtgatgtag ttttattatc accagcgaat
    28741 gccagctggg atcaataccc aaattttgaa actagaggaa acagattcat ggaagctgtg
    28801 aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt tggttacagg
    28861 cggaggaaca gggggacata tttatccagc tcttgccttt gtcaattatg taaagactca
    28921 agaaccgaat gctgaattca tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt
    28981 ccctgataca ggaatgcctt tccatacctt ggagattcaa ggatttaaac gaaaaatttc
    29041 catgcataac atcaagacgg tccgattgtt tcttaagagt atccgtcaag caaaaaaaat
    29101 cctcagtgat tttcaaccag atatcgttat cggtactgga ggatatgttt ccggagcggt
    29161 agtgtacgct gcagccaaaa tgggtattcc aacagttatc catgaacaaa acagtgtacc
    29221 aggtgttacc aataaatttt taagccgata tgtggatcgg atcgctcttt catttaaaga
    29281 tgcggcttcc ttttttcctg aaaacaaagc agttcttgta ggtaatcctc gagcacaaga
    29341 agtagcagat actaaaaaat cagatatttt gaaaacattt gatttggatc cagaaaagaa
    29401 aacagtcttg atttttggcg gtagtcaagg agcacttaaa atcaatcaag cagtatcagt
    29461 ttttttaagt gaatatattc cttcttctta ccaagttttg tatgcttcag gagatagata
    29521 ttatgaagag atcctaaata aaataaatcc tgaaatacaa aatattagtg tccagcctta
    29581 tatcaaaaat atggcagagg tcatggcttc cagtgatctg ctggtggggc gtgctggtgc
    29641 aacttcgatc gcagaattca cagctctggg gttaccggca gtgttggttc ccagcccata
    29701 tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa
    29761 aatgattgca gataatgaac taacaggtga atcactaagt cagacagtca acgaaatcat
    29821 gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag gaatccctga
    29881 tgcttcaaaa cgtctttatg acttagtaaa acaaataatc aaaaaataaa ggcggtggtc
    29941 ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat
    30001 ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag aacaagagat gaaaaaccaa
    30061 gagccaaaaa aagaagacat aacattagat acaccccttt cagattctga agaagaaaaa
    30121 acggttcaag aggaacagca agaagaaagc cagtccgaga aaaaaatcga attttttgaa
    30181 gagatcaagc aaaagaaaaa cggtggacca tataacggtt cttttttgaa ccggctgccc
    30241 aatttaaaaa gtcaacggca caaagtacta tttcgtcggt tagctttgat catcactgta
    30301 ttgggtatac cgctgatttt tttgatctat tatgtttctc cttatagcaa attacaagca
    30361 gttgcagtat caggcaataa gacagtcaac agccaagaaa tcatctctga tacaaaattg
    30421 tcattaggag aaaatgtttg gggacagtat tttcatcgat ctacttatat cgatcgcctt
    30481 aaaaaagcac agccaagaat cgaaacagca aatatacatt tcaaagggat gaatgaattc
    30541 gatttggatg taacagaata taaagagatt gctttgatcg caaaaaacaa tcaatactac
    30601 ccagtgattg aaaatggaac agtattagat gaaaaagtag caaatcctac aaaaaatctc
    30661 cctatcttgg aagagttcaa agactctgca aaaatcaaag aattaacaaa gcaatacaat
    30721 cagttatcat cagaattaca aaaagcaatt tctgaaatca agtacacgcc aagagcaagc
    30781 aataaaaatc tgatccaatt gaatatgaat gacggcaatc aagttattgt aaatatcaat
    30841 aatcttgcga accaaatgaa atattattcg caagtagcca aagatatgga tgaaaaaggt
    30901 gtgatcgaca tggaagttgg tatcttctcg catccttacg gtagcactca agaaacggaa
    30961 gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa atgcggatca aacaaacgaa
    31021 tcaagtgaag aggcagaaac gcaaaaagca caaacacctg gatcgtctag cacaaacgga
    31081 tcatctgatt caaatgattc aagtgaaaca agtcaaatag aagataatcc aagtacaact
    31141 ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa agtaagaatg tactttctaa
    31201 aaaccagtaa tttatggtag aattggaagc agtgaatata tctattaata agtatatttt
    31261 aaaacggacg ctagttgttc gcttgtatat ggaatagtaa gtaggaggga atcccattca
    31321 tggcaaaaac aggaatgtat gtaggccttg atattggaac gacatctgtt aaagttgtcg
    31381 tggctgaata tatcgacagc caaatgaata ttattggagt aggaaacgca aaatcagaag
    31441 ggattaaccg aggcattatc gttgatatcg ataaaacggt ccaagcgata caacgagcag
    31501 tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag
    31561 caaacatgct tgaagtagaa aattgccagg gaatgattgc agttaacgga gattcaaagg
    31621 aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc
    31681 ctgaacgtca aatcgtgtca atattgccac aagattttac tgtcgatggg ttcgagggaa
    31741 tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat gtacggtctg ttgtttacag
    31801 gacctaaaac gatcatccat aacatacgta aatgcgtgga aaatgccgga ttaatcgtca
    31861 acgaaatggt gattactcct ttagcgctga ctgaatcaat tctttctgac ggagaaaaag
    31921 attttggtac gatcgtcatt gatatgggtg gcggccaaac aacaacggct gttatgcatg
    31981 ataagcaatt gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc actaaagata
    32041 tctctattgt attaaatacg tcatttaaca atgcagaagc gttgaaaata aactacgggg
    32101 atgcataccc agaacggaca tcaccagatg aagaatttcc agtagatgtg attggtcagt
    32161 cagaaccagt gaaagtcgat gaacgctact tgtcagaaat tatttcagct cgaatggaac
    32221 agattttcaa caaagcaaaa gatgcattgg accaaatcga agcattagag ttgccaggtg
    32281 gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa
    32341 tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga aacccggtat
    32401 ttacaaatgt aataagtatc gtggattatt cagcaaactt gagtgaagtg tatcaattgg
    32461 caaaaattgc tgttacgggt gaaacctcag cagctcgtca aatggtagtt gaacaagaaa
    32521 caacaaacac atatgagagc tatgaagcac ctgaagaaac aatctacgat gagccggaac
    32581 caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg tttctttaca aatatttttg
    32641 attaattcat cctgggagga ataagatatg gaattctcaa ttgataataa tatcaatgac
    32701 ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg
    32761 attgaagaaa acgttaaagg tgttgaattc attacagcca acacagacgt gcaagcatta
    32821 aaaaattcaa aagctgaaac agttatccaa ttaggcccca aatatactcg tggtttaggt
    32881 gctggttctc agccagaagt tggacaaaaa gcggcagaag aaagtgaaca atccttacgc
    32941 gaagctttag acggtgcaga tatgattttc atcactgcag gaatgggtgg aggaaccggt
    33001 acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt
    33061 gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt
    33121 atcgctcgtt tgaaagaaaa tgtggataca ttattaatta tctcaaataa ccgtttatta
    33181 gaagttgtag ataaaaagac accgatgctt gaagctttcc gtgaagcaga taatgtttta
    33241 cgacaaggtg tacaaggtat ctctgatttg atcacagcgc caggttacgt taaccttgac
    33301 tttgctgacg tgaaaacagt gatggaaaac caaggtacag cattgatggg gatcggtgtg
    33361 gctagtggag aagatcgcgt agtagaagca acgaaaaaag caatctcctc tccattgctt
    33421 gaaacatcta ttgatggagc tgaacaagta cttcttaaca tcacaggcgg tttggatatg
    33481 acattgttcg aagcacaaga tgcttcagat atcgtagcaa atgcagcaac tggcgatgta
    33541 aatatcattc taggtacatc tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc
    33601 atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc agctagacaa
    33661 gcacaaatgc aatcacctgc tcaaaagact gtattagata tggatcaagc taaaccaact
    33721 tcttcagaag aagaaaacag ttttggcgat tgggatatca gacgtgaaca aaatgttcgt
    33781 cctagagttg atgattccaa ttttgacaat atcgaaaaaa aagaatttga tacattcaac
    33841 cgcgaagaaa caaaatctca tggcgatgat gaattaaata cgccaccgtt tttccgtcgt
    33901 aaaaaataaa ggagaggaag agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga
    33961 aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat tagtagctgt
    34021 aacaaaatct gttgaatccg ctcaagctga agaattggtt agagctggcg ttatgaattg
    34081 tgcagaaaac cgagtagata aattattaga aaagaaacaa gatttatttg attttcctga
    34141 aataaagtgg catttaattg gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga
    34201 aattgattac tttcatgcgt tggacagttt aagattagca gaagaaatcc agaagcggtt
    34261 agagaaagaa ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat caaaacatgg
    34321 aatttcacca gatgagcttt taccgtttgt ggaatcttta gctgcctgca caaaaatcaa
    34381 ggttgttggg ttgatgacta tggcgcctaa agaagcttct gatgagcaaa tccataaagt
    34441 gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg cccatgcacc
    34501 atgtagtgaa ttaagtatgg ggatgagtca agactttcaa attgcgattg aagaaggtgc
    34561 aacatttatc cgtgtaggaa cagcgctatt caaagacgaa taaaggagga acaaaaatgc
    34621 cgcttttaaa cagaggagca attgctaatt ttttcggatt agctgatgag gaagagtacg
    34681 aataccaaga ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa
    34741 cacagaatca atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg
    34801 cgacttcacg tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa aaagtaattg
    34861 aactacatca atcacctaca aaaactgtag cgaaagaaag aacagcttca agaacctcta
    34921 cacaacctaa agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga gcttattctg
    34981 aagctatggc gattgctaaa cgaatcattg ctggagaagc aaccttagtg aattttcatt
    35041 tagtagaaga aaaacaagcg agaagaatcg ttgattttct aacaggaaca gtttatgcac
    35101 tagatggaga tatcaaacgt gttggagatg agatttttct ttgtacaccg actgggatgg
    35161 agattgacag ttcaaccgct caatcttttg cagatgcgaa tttgtttgat ctgtaagagg
    35221 aggaactacg atttatacta ttgcactatt acttcattta cttcaacggg cagcctattt
    35281 ctatagcatt atcttgatgg tatatgcatt gctctcttgg tttccaggcg ggtatcaatc
    35341 agggctagga cgattattag ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct
    35401 gcctttgagg attggaccct tggatttcac aattttaggg gcaatcctga tcttgaactt
    35461 agccgtacaa ggtttagcac agatcgttat atggataact gtccatttag tttattgatt
    35521 gaagcaacaa aatctttatt ctcagaatag ataggagggg aaaattttgg atgcaaatgt
    35581 ataccagcac tttcgggcag atgaacgtac atttatcgat tcggtaggcg attggattga
    35641 acaggttcaa ggacaatatg caccctatct gaccgaattt cttgacccaa gacaatccta
    35701 tattttagaa acactgattc gacaaagcag tgatttgcgt ttcatgtttt atggcggata
    35761 tgaacaggca gaaagaaaaa gatgtttgat tttccccgat tattacgaac ctgtagagga
    35821 agattttgaa acagcattgt atgaaatcca ttatcctagc aaatttgcca gcctaagtca
    35881 tgggaaaatc cttggaactt tagtgggaac ggggatcaaa cgctcttact ttggcgatat
    35941 catctcggac ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc attttgttgc
    36001 tgcacaagta acaaagattg gaaaggttgc tgtacgcttg gaaccaatca aatacacaga
    36061 aatgattatg ccgaaagata gctggacaca agaaagagga acagttagtt ccatgcgact
    36121 agacagtgtg atctctgaga tttataatat ttccagacag cggtccaagc agctgatcga
    36181 agctggtaaa gtaaaagtga actggacaga aaatacaaga cctgattttg tacttgaact
    36241 attagatatt atttcagtca gaggttatgg cagagtccag atccaagaac ttgaggggaa
    36301 gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt
    36361 ggtttactca tggctttaac tccattagat atccaaaata agacattccc gacaaaaatg
    36421 cggggatata atcaagacga agtagatgat ttcttagatt tagttgttcg tgactatgag
    36481 gaattgactc aaagaaatcg agaattagaa aaagcagtca aacattctga agaaaaactt
    36541 gagtacttca atgaattaaa agatgctttg aaccaatcaa tcatcgtagc tcaagatacg
    36601 gctgataaag taaaaacaag tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa
    36661 aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat
    36721 gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag acagcttgcg
    36781 acagaaactg aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg
    36841 cttgagtctc aattagaaca agtgaaaagt ccggaatggg atgaaatttt acaaccattc
    36901 tccagctacg tgactgacag ccatgaagtg atcaaagaag ttttagcaaa acagcttgac
    36961 aatgaaaatg atactgatgt aaactcagac acaggagtca tagaagcacc tatgacggca
    37021 tttgatacac aggctattga tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac
    37081 agaaaagcac tttgttattc ttaaagcgag ccgatgagag tggaagttcg gtaagacact
    37141 gcatggtgaa agatcctcta cttgagacct gcttttccga aattagagta agaagcagcg
    37201 gctgacctcg ttattggtca cagagagttt gcactagata gatgcaacta aatttgggtg
    37261 gtaccacggc atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat
    37321 aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc
    37381 cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat
    37441 cgaatctacc aaaaaagaca agagttgaac gaaggaaaac ctagctttgt tcttcatgat
    37501 ggacctccat atgccaatgg taatatccat ttaggacatt cattgaataa aatcagtaaa
    37561 gacattatca ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt tccaggatgg
    37621 gatacacatg gattgccgat tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa
    37681 atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg cactttccca agtagataag
    37741 caacgagcag atttcaaacg cctaggagtt gcaggagatt gggaacatcc ttatgttaca
    37801 ttagatcctt cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaggc
    37861 tatatttaca aaggattgaa accaatttac tggtctccgt caagtgagtc ttctttagcg
    37921 gaagctgaaa ttgaatataa agatgtaaaa tctccatcta tttttgtagc tttcaatgtc
    37981 aaagacggca aagatattct tgatgaagat acagcttttg tcatttggac gacaacacca
    38041 tggacattgc cagcaaacca aggtattgct gtaaatccaa aatttacgta tgtattggtg
    38101 gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa
    38161 atcggttggg aaaatgtaac agttctaaaa gaatttgctg gacaagaaat ggaatatatg
    38221 actgcaagtc atccattcta tgaacgtgaa tcacttgtga ttttaggcga tcatgttact
    38281 ttagacgctg gtactggatt agtccatact gctccaggac atggggaaga tgactatatc
    38341 gctggtagca aatataaact atctgtagtg tcacctgtag actcaaaagg attgtttaca
    38401 gaagaagcac ctggatttga aggtatcttt tatgacaaag caaatcctat gatcactgat
    38461 ctattaaaag aaaaaggagc tttgctgaag ctagatttct ttactcatag ttatcctcac
    38521 gattggcgga cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt tgcatctatc
    38581 gataaattcc gtcagaatat tttggatgaa gtagaaaaag tagagtgggt tatcccatgg
    38641 ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg attgggtaat ttcccgtcag
    38701 cgtgcatggg gagtaccact tccgattttc tatgctgaaa atggagaagc aatcatcaca
    38761 ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac atggatcaat tatctggttt
    38821 gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc acccaggttc tcctaatggc
    38881 gaatttacaa aagaaaaaga tatcatggat gtctggtttg attctggttc ttctcatgaa
    38941 gctgttttac gtcagcgacc agaattatca ttcccagcag atatgtattt agaaggatca
    39001 gaccaatatc gtggctggtt caactcaagt atcacgacta gtgtggctat caatggtgta
    39061 gcaccataca aatcagttat ttctcaagga tttgctttag atggtgaagg ccgtaagatg
    39121 agtaaatctt taggaaacat cattgcacca gaaaaagtga tcaaacaatt tggtgcagat
    39181 atccttcgct tatgggtatc tagtgtagat tatgaagcag atgttcgtgt ttctatggat
    39241 atcttaagtc aagtggcaga agtatatcgt aaaatcagaa atacgatgcg tttcttatta
    39301 gcgaatacag aagatttcga tcctaaaaaa gatactgttt cgtacaatga tttacgttct
    39361 gtagacaaat atatgaccgt tcgattgaac caagtaatca aagaaatccg agaagaaggg
    39421 tacgacaaat ataacttcat gcatatttac cgtacagtta tgaacttctt gacggttgat
    39481 ctatcgtcat tctacctgga ttttgctaaa gatgtagttt atattgaagc agaagatgat
    39541 tatcaacgtc gttgcatgca aactgttttc tatcaaacag ctgtagcttt gacaaaatta
    39601 ttgactccta tcattccgca tacagcagaa gaaatctggt cattcctgaa agaagaagag
    39661 gagtacgttc agttaagcga gtttcctggt tatgaagagt ttgcaaacca agaagaattg
    39721 atggatacct ggactgcttt catggacttc cgtgataatg tcttaaaagc attggaagaa
    39781 gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa aactaactgt ttatccaaaa
    39841 caacaagttc gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt attgatcgtt
    39901 tcaccagact actttgaagt tatggcagca gatgaacaag tgccagataa cgctatggta
    39961 tttgatgatg ttgctattac agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc
    40021 caagttcgta aagatgtagg agtagatgaa aaacttcctc atctatgcgg tcgttgtgca
    40081 aaaatcgttg aagacttcta tcctgaagct gtagctgaag gattcgaaga aaagtaaaag
    40141 ggaaaagggg gtgtgacatg ttctttgtca cacccccttt tgttatcaat cgtttaattt
    40201 tccacgttca cgataccagt tatctggttg ccagatccat tcgaatccgt ctttttcaag
    40261 tagttcgaaa gctgctttag gtcccattga tccagctttg tagttgggaa aatccggttg
    40321 tgtttggtcc caagcttttc gaatgacatc cacgatatgc caagattgag cgacttcttc
    40381 ccaatgagag aagttcgttc catcaccatt caacgcatct aatagtaatt tttcgtaagc
    40441 ttctgggcta ttttcaacca tttctgcact atttctaaat tccaatttga ttggttccgt
    40501 ttcaaatcct tgccctactt ctttcccatt caaggataaa gaaaatcctt cagttggctg
    40561 aatatagatc gttagaacat taggtggaag tgtcgtatcg tcacatggtt catcgacaga
    40621 tgttttgaat acattaacag gaacttgttt gaatacgatg ttgatccgag tccctttttc
    40681 agtcaaacgt ttgcctgtac gtacatagaa aggaacacct gaccaacgga agttatcgat
    40741 agtgaatttg cctgcgacga atgtttcagt ctctgattga gcatccactt tgtcttcttc
    40801 gcgatagccc ttgaattgta caccatccaa ttcaccttga gcatattgcc cgcgaacgaa
    40861 gttttctaac acttcttctt cgttataaat gcggatagct ttcaatgctt tgatcttttc
    40921 tgtacggatt tctttttcgg aaaatgctac tggcggttcc attgcaagta atgctacaac
    40981 ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc
    41041 gcgatcttct acaccgaggt tttcgccaaa agtaatttgg atattgtcga tatagcgatt
    41101 gttccattga gcctcaaaaa tattattggc aaaacggata gctgagatgt tttggatcat
    41161 ttcttttcct aaataatgat caatcctgaa aatatcttgt tctgggaata cttctctgat
    41221 ttgctggttc aattcaaaag cagactggta atcagaaccg aacggttttt ctatgatcaa
    41281 acgattgaag ccattttttg tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc
    41341 aaaaaattga ggtgccatag ccaaataata gacacggtta ccgtttaatt gatagtgtcc
    41401 atctaatcgg tcagacaatt cttttaatgt ctgataatgc gttgaatcat ttacatcatg
    41461 ggattgatag taaaaatggc ttgcaaaggt agttgcttct tcatctgaag gattcaatgc
    41521 acggattgtt tctttgacaa cttcacggta gtgctcatcg ctccagggac ggcgagcggt
    41581 tccgattaca gcaaaatgct cgcctaaatt tccttttctg taaaggcgga acaaagacgg
    41641 gtacaattta cgctgggcta ggtcacctgt accgccgaat atcgtaaaca atacattttt
    41701 ttcattagac atgtggatac actcctttat attttatcga caaaaatcgt gctggccgcc
    41761 ttaaagctga cggggatttt ctttctatca ttttggatag tgatcggtcc ttcataagct
    41821 gcgatgtcaa cgatctcata ttcttcgttg atattcaggt caatagtgac taagtaatcc
    41881 aacaattctt tttcatcaag cactcgtgcg atacgcactt ttgtaccgac aggataggac
    41941 tccaatgttt gtcggcgttc ttcatggaca acttcctctt ttttaggaat cgatcctcca
    42001 tgaggacaat gttcaggatg attcaaatag ttttctaaat gatctgcaag atgttccgaa
    42061 gtcacatgtt ccaatacttc agcatcatca tggacttcat tccagctata gtctaaatgt
    42121 tcaactaaaa aaacttccca taagcgatgt ttgcgaatca gactgctagc tttttgcagt
    42181 ccgcttgcag tcaattgtac accttgataa ggggaatgtt ctacaagctc ttctttcaca
    42241 agtttagaaa tcatttcact gacagatgca gctgagacat ctagactaga tacgatttgt
    42301 ttattgttga ctttcgttgt atctccacct agttctaaaa tgattttcaa ataatcttct
    42361 cgatttggtg tcatattcga tcatcctttc atggtaaaaa accatcatta gtaagtgtaa
    42421 caaaaaaaac gttaattgac tatttttcta aataaaaaac aaaggatttt tagacgtttt
    42481 tacaacaaaa aacgaaagat gatagagaag actcattctt tcgtttataa aggatatata
    42541 tatacattgg gagggatcta tttttcagat aacaacttat atgaagtgtc tgcagattcg
    42601 tcataaagag tttgttcggg ataagaaaag ataagataaa aaccatactg atcacgtacg
    42661 actaaacgat attcatctat ttctaccaca gatgtgccaa gagactgacc gttgatcaaa
    42721 aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct ggctatcact aaagaaccat
    42781 gtaccttgat actttgagag tccttctgac ttttccgctt gttttttctt tttcaagtaa
    42841 ataaaacttg agagaacggt aatgagtgaa ccaagaaaat agatcatttt ttttatcacc
    42901 aaatcactcc tttatgagaa aataatcgca tagacatatt ctttcttctt tatttcattg
    42961 taccgaataa aagctaaaat ttctctaaga ctttcgactg gtttttttac tgtttttcta
    43021 caaattatga taggatacat agtaggaaga aaggtgacat aatttgaagt atcagcaagt
    43081 ggaaatagct ttttttattg accgcccgaa tcggtttata gcacattgta tgaaaaaaga
    43141 cggagaaata attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc
    43201 aggcgctgaa gtggctattg tatttgctcc tggacctaaa cggaaaaccg cctatgatct
    43261 gattgctgta aaaaagaagg gtgactggtt taatatcgat agtcaattgc ctaatcggct
    43321 ggcaattgat ggtattttag acggcacgat ccttttacca ggattaactg gtccatttac
    43381 ggtatacaag cgtgaagtaa cttatcttca ctccaaattt gatatttatt tagagacggt
    43441 ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg
    43501 tgcttttcct gatgcgccca cactcagagg attgaaacat ataggagaat taacgtatgc
    43561 cgcacaagat ggttatgccg cttatgtctt gtttattgct cagttcgaac atcttcatct
    43621 ggcaacgatc catgaagaaa tgcaaccagc actagctgat atggttcgac atgcgcagca
    43681 aagcggagtt caaatcctcg cctataattg tcaagtatcg gcagatgagg taactgtcaa
    43741 gcaggccatt ccatttgatg tagatatccc ttttgaagat cctatacaat agaaagaagg
    43801 taaaataatg atcaacttag gtattatcgg aacaaattgg attactcatc aatttgtcca
    43861 agcagcttta agcacaaaaa aatatgaatt gacagcggta tattctagac atttggaaaa
    43921 agcacaggaa tttggttctc attatgaagg ggatatcgaa ttcgcaacag atttgaagac
    43981 ttttttcaat attactcata tggacaccat ttatattgct tctccgaata gtcttcactt
    44041 tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa agccagcttt
    44101 ttcgacgcca aaagaaatgg atgaaatcat ccatttggca aatgaacaca atgtgtattt
    44161 ttttgaagca gcaagaaata tccatgaatc aggttttaaa aagattgccg attacttacc
    44221 attaaaagac gagataatcg gtgcaaattt cagctatatg aaatattctt cgcgctatga
    44281 ccaagtatta gatggagaag aaccgaatat cttctcgact catttctcag gtggtgcatt
    44341 gatagattta ggtgtttacc ttgtatatgc agctgttgct tggtttggaa tgccaaaaga
    44401 agtccattat ttcccaagaa agatttctac tggtgtagat ggtcttggat gggggatctt
    44461 acgttatgag aattttgatg tatcgatcca gccaggtaaa atcggtgatt cttatttacc
    44521 gtcagagatt tattttgact caggaacgct agtgatgaat ggagtcaatg caattgaaca
    44581 agcacaattt catgatcgcg cgcataaaga aatcattgat ttggcggtaa cagctgcaga
    44641 aaatccaatg gcagatgaag cagaggattt tgccaatgtc atggctcatc cgaaagatcc
    44701 agcttgggga ttgttgtatg aagagtggat cgagctagct agaaatgtga accaagtgat
    44761 ttatgattta agaaaaaatg gcggcatcaa attcgatgca gataatgagg aagattttta
    44821 atgaacgaat ttgtacaagt atttcccgaa aattggcaga aaaaatatga ggataaaggg
    44881 tttatctcac cttctcttat tcaacgagca gtttttcagc cactaagtaa taaacaaagt
    44941 atagtagcgg tttctcctac aggttcaggt aaaacactgg cttatctttg gcctctacta
    45001 ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta
    45061 gctattcaag tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa
    45121 tctttggtgg gcggagccaa cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa
    45181 atcttgattg gtacaccagg aagaatattg gaattgatga aagcaaaaaa aataaaagca
    45241 catcaagtga aaacaatggt atttgatgaa gcagaccagc tatttgacgc tgggaacagc
    45301 caaatcattg atcaaattct tcatcaagct ccaacagagt atcagctagc ttttttttca
    45361 gcgacagcag atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca
    45421 attgacgtga cagcagagga cgattctcga aaaggactga gacattactt tttacgtgta
    45481 ccgatacgaa agaaagatga ctacttgaga cgtctcacgc atatagaaga ttttcatggt
    45541 ctgattttct ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt gctgtataga
    45601 ggtgtaccgg taggaagtct ggcctctgat cagaacaagc tactccgaaa agcagcgcta
    45661 gaacaattca aaaagaaaat ggtcagagca cttttgacca cagatgtggc ggctagaggt
    45721 ttagatatta caggtcttcc ttatgtagtg aatgcagaag ttccgttaag tgaagaagct
    45781 tatttgcatc gttcgggaag aactggtcga atgggtaatg aaggaatagt aattacactc
    45841 gtacaagata ataatctaag agatttaaaa cggcttttac gtccatcaaa tataacactt
    45901 gaagagattt ttttatacgg aggtaagctg caaactgagc agccaaccaa agaagatgca
    45961 gctcctgcag taaataaaaa gtatgcgaaa actttttctg gaaaggagca gaacccagaa
    46021 tataacaaag aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa
    46081 ggagcaaggc gcaaataagc gcctttttat gctatgatta tgaaaacggg aacaaaattg
    46141 gttgccagag gaggggttat gtgaaagaac gtcaagaaga gatcttgcag ctattgacag
    46201 aaaaccaaca aggattgaca gcaagtgatg tagctgagcg cttaactatc gatcgaagca
    46261 atgcaagccg atatttaagt gaactataca aggcccatca tattgtaaaa accgctggcc
    46321 gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact
    46381 cttctacgca agtgactttt gaaacactcg ttggagaaaa tgattcttta aaagtcagca
    46441 tacagcaggc aaaagctgcg attctttatc caccgcgggg gttgcatacg attatttttg
    46501 gagaaactgg tacagggaag tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt
    46561 cagagatgct ttctgcagat gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa
    46621 atccgcaact gctctttgga cacatctttg ggatcaaaaa aggggcttac acaggagcag
    46681 ctcaagatag tcccggtcta atagccaaag cagacggcgg tatattgttt ttagatgaga
    46741 tccaccgttt accgccggaa gggcaggaaa tgctgtttac tttcattgat aaaggtattt
    46801 accgtccgct tggagaaagt gcacaagtac atgaagcatc tgttcagatc attggggcga
    46861 cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg acggattcct atggcaatca
    46921 ccttgccgaa tcttgcttca cgttcactgg atgagcgtta tgaaatcatc tctcttttta
    46981 tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg
    47041 ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac ttgaaactag
    47101 tctgtgcaaa atctttttta cattaccgta ctcatcatga gaaaaagcta atcattcgaa
    47161 aagaagaact ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa ataccggaac
    47221 gtctcgaccg attcatggac agcaaaagtc agtatttgac atttgaacca ggatttgctg
    47281 atgttgtctg gtcacaagat ccagaacgaa acatgcaggt ctataacgat attgaggaaa
    47341 aagtcacttc tctttccgaa accggtgtgg agaacattga tctagagacc ctcatttcaa
    47401 aagatgtgga tgcttatttc cagacatatg tcaaagaatt gactaaaagt tcgattccca
    47461 aagatctttt gccagatgaa atctggcggt taaccaatca attgtatgat gtggctgaaa
    47521 aacggttaga ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa
    47581 gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg aataacgtac
    47641 ggaaaaattt taagagggaa ttccaggtag caatcgatct ttcaacgatc attgaagaag
    47701 aagaacaagt tgagattcct tttgatgaaa tcggctttat tagtatgttt ctttctatca
    47761 atctaggaga aagtgagcct gttaaagaaa acaaagtaga tgttctcata ttgatgcacg
    47821 gcagacagac tgcttcaagc atgttggaga tggcacaaga actattagga acaaaagtag
    47881 ggaaagcttt taatatgcca ctggttatgg aagttcaaac aatgtatgaa gaagtgttac
    47941 aatatgtcaa aaactatcaa gagcatttga cgaatggcct actcttgtta acagatatgg
    48001 gatcattaaa caccttcagt gatttgattt atgaagagac tggcattcgg acgaaagcta
    48061 tcacgatgac aagtaccatg attgtgatag aagcgatacg gatggcaagt gttggaagaa
    48121 gcttagaaga tatttatcaa aatatccagc tttcctttga atcgatcgta cgtgatcagt
    48181 tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg acatgcttta
    48241 ctggtgaagg agtagctgcc aaattatatc agcgtattat gccagtgatt aatcaaaata
    48301 aggtagaact gatccagatg cagtttttag aacgtgaatc attcaaaaaa cacatcgatg
    48361 ccttattgga agaatatgag atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa
    48421 atatcccatt tttttcagcc tatgacgttt ttgatgatga aaaactaaat attttaaagc
    48481 gtatagctga tgatgagatc ccgacagcta aaattgtcga ttcacttgct ggatcattaa
    48541 gcgatgtctt ttctattgat gaactgatcc gttcattgca aaagacagtt cagcagatcc
    48601 agaccgattt gtcgattatc gtggaacctg gagtagatac tggaatcgtt atccatttag
    48661 ccttcatgtt agacggattg atcaaacaag aaagccatcg gcaatttgag aaactgtcta
    48721 ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg atggcactag
    48781 aacgaaaata tcaggtaaaa attccagaag atgatatcgc atttcttaca caaatgtttc
    48841 ttgaaaataa aataaaaaaa tgaacacact aatcatactg tttacacact aaacatctgt
    48901 gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct atttcagctc ttttttttat
    48961 tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca
    49021 aaaaaaacaa tcatgttagt ttgttcagca gggatgagta cgagtttatt ggtcacaaaa
    49081 atgcaaaaag cagcagaaga aaaagggatg gaagccgata tttttgcggt ctctgcctca
    49141 gatgcagata ataatttaga atctaagaat gtcgatgtat tattattagg tcctcaagta
    49201 cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc attagatgtc
    49261 atcaatatgc aagactatgg catgatgaac ggagaaaaag ttcttgcaca agcagaaaat
    49321 ctaatgaaat aaatcaagta tgcagttggg ggtataacaa cgtggaagat caacaaaatc
    49381 tagaagccat catggggtta atcatgtttg gcggtaatgc aaagagcgat gcaatggaag
    49441 cgattgcggc tgctaaaatc ggtgattttg agttagcgga taggaaaatc gctgatgcag
    49501 aagaatcact tatacaagcg catcatgcac aaacagacat gttgacacaa gaagcacaag
    49561 gaaatcatat gcaagtgaca ttattgactg ttcacagtca agatcactta atgacatcta
    49621 ttgcgtttac tgatttagca aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat
    49681 aaatacaact tcttttttga acggaagaag aaaaattttt gaacggattt tttattttgt
    49741 tataaggaga actctcttta tataaaaaac ttataggagg aagtaaaaaa tgaacggact
    49801 atcaagttgg atggagaaat acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca
    49861 tttagttgct ttgcgtgatg cttttatcgg cacactgccc gcaacaatgg cgggatcagt
    49921 cgctgtaatg atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca gtacctatgc
    49981 tgacagttta gcagcagaca aaggaatctt tgccgtattg aatgtcatca tcggaatcaa
    50041 tggttttgtc tggaacggta cactagcaat cgccggattg atctttgcct tttcttgggg
    50101 gtataatctt gcccgagctt atggagtaaa tgatctagct ggggggatcg ttggtttagc
    50161 taccttgatt caaggtattt cctttggtcc agcattatca gcagcattag atgtgaatgt
    50221 tccttcaaat gtcgcagatg caatcaatag ttctgagatt ggtgcaacga ttgcagatgg
    50281 cacactgtca gttggattat ggggttggct aaaactagat catttgaatg gaaacgctta
    50341 ttttacagtt atgatcatgg gagctatttc agtcattatt ttttgtaaat tgatgcttgc
    50401 caacattacg atcaaaatgc ctgattcagt accaccagca gtatctaaag catttgcagc
    50461 cattttacca gcgacgatcg ctttatacgt cattgctatt atcaattata tcgtaggtcg
    50521 tgtagctgat gggcaactga tcattgattt ggtgcaaaaa tatattgcag aaccattcct
    50581 aggattatct caagggttgg gagcagtatt gatcgtcaca gtatttgttc aagtattctg
    50641 gttctttggt attcatggac caaatgtatt ggcaccagtg ctagagggaa tctggggaca
    50701 agcacaacta atcaatattg atattttcca aaaaggttat aacggaaaaa ctggtacacc
    50761 agccgtgtta gatgcaatcg aagatggaaa agcttatatg tgggtccgcg gatcgttcga
    50821 tgcttttgca tggtttggcg gatcaggcgg aacgatcgtc ttactgatag ctatcttgat
    50881 tttctctaaa cgtgcagatt atctaacagt agctaaatta tccattgggc ctggtatctt
    50941 caatatcaac gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat
    51001 cccattcttg attgctccag tagtagcaac agcaattgga tggacagcta cttatcttgg
    51061 cctagtcgct ccggtatcgc agcaagtcac atgggtagta ccgccgttct tattgtcatt
    51121 cttagcaaca ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac
    51181 tttcttgatt tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga
    51241 acaacaataa gatgaacgtg aggagcggga cgctttaagt ttcgttcctc ataatgaatt
    51301 ttcgaggagg aactgaaatg attacaattg ccagtgcaat gcaacaagat gaaatcaaag
    51361 aattgttaga aggatacaat gaagaaggaa tgacttttac atttaaagaa aaacaaggaa
    51421 tcaagctgtt ctttgaaaca agtgcagaag atccggaagt tgcggctaaa aaagcaaagg
    51481 aactaatcaa agcacagcct tggggaacag tcctttattt tcaatcaaca gctgtttaga
    51541 aaatggttta aaaagatagg atggttgaga tgataaaaaa cggtttattt ttatgtagta
    51601 ttggttcttt gatcgtgttg tatagtttga atccacattc gcaaaggtat gatttgtata
    51661 gtatggtcac aggtcttttt ctaacaggat taggaatcta tttcttcatg aaggggaaaa
    51721 aaagagaaga agcaaaggag aaaaataact aatggaaatc acgaaaaaat taaatattcc
    51781 agcatcgttt ttttacgata aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac
    51841 cggcaaatca gtaactagaa aacagctgaa aaatttggag tacgtcaaac aattctcgca
    51901 aaataaccgg gctcgcataa aaatcgaaga agtcattgaa aaccagtctt ataagtttcg
    51961 tacatcgaca acgaagaatg aattcgtcgt tcattatcaa atcaaaccat tggacgattc
    52021 gacctgtgag gttcattaca cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa
    52081 tgatatgctt ttaggaacaa tcttgatgta ttttaaacgg agacgtttca aaaaaatgct
    52141 agatatgatc gaagcaacgt attaaatcaa tgaaacatgg tactatttag ttattgaaaa
    52201 atgaagtaag ctagaaggaa tttttcatag gaagatagat ctttcctgcg aatcctcact
    52261 agcttttttg taggaagggg cagcaaaatg acaaacaaag taagagagct aggtgtatct
    52321 tcgattgagg aaatgtttga tttagccact tatgcattta atggggcaga tacacctgaa
    52381 aggcgagaac gttttcgtac acttgcggag aattcttgga attacggatt ttttgatgag
    52441 gaggatcggc taacaagtca agtcatggcg accccttttc cagtgaattt ttacggacaa
    52501 gagtacctaa tggccggcat tggctatgtt gcttcttatc cagaagcaag gggacaaggc
    52561 gggatcaatc agatcatgga aaagatcctt gaagattgcc gtgatcgtaa agtaagctta
    52621 tcttatctgg cgccattctc ctatcccttc tatcggcgct atggctatga acaatcattt
    52681 gataaaatca gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga
    52741 aaaataaagc gtatggattt tgaagatgca aaagctgcaa tcagaagaat ttatgaacag
    52801 atgccggaaa accaacgagc tggtctgaag cgcgaagact ggtggtatac gtataaattc
    52861 aagcaaaaag gcaattacca gtttgctttg cattttgatg ataatgagga agcaacggga
    52921 tatatcgttt atcagcttgc aacgtcttcc tttattattg cggagtgggg atttttggat
    52981 catgatgctt tttgcagcct tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca
    53041 tttgaatatt catgcggcta cggcggtaat gatcaaaatt atctattaga aaatccattt
    53101 gcttctgtaa tgatcacacc ttatatgatg gcgaggatcg tagatatttc tttgtttttg
    53161 gaaaattatc catttaaaaa gagacaagct gcatttgccc tacaaataga agaagatcat
    53221 tatgcgaaat ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc
    53281 tcaaaagtag aacaaacaaa actcccaatc gttcgaggaa gtatccaaca tatcacgcag
    53341 ctattattag ggtatcgtaa agtggaagag ttggtattcc aagaaagaat acaggtgaca
    53401 aaagaatatg aagaaacgtt aagctcaatc ttgccacagc aacagccaat tcttaatgac
    53461 tatttttgat aaaaaacggg tgatgacaat a
//
LOCUS       DACEPD010000010        53462 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.010, whole genome shotgun sequence.
ACCESSION   DACEPD010000010 DACEPD010000000
VERSION     DACEPD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.010"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            135..1454
                     /locus_tag="IW797_000551"
     CDS             135..1454
                     /locus_tag="IW797_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ1223348.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDKRAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(1660..1758)
                     /locus_tag="IW797_000552"
                     /pseudo
     CDS             complement(1660..1758)
                     /locus_tag="IW797_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
     gene            complement(1773..3020)
                     /locus_tag="IW797_000553"
     CDS             complement(1773..3020)
                     /locus_tag="IW797_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1223349.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(3096..3242)
                     /locus_tag="IW797_000554"
     CDS             complement(3096..3242)
                     /locus_tag="IW797_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ1223350.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(3346..3657)
                     /locus_tag="IW797_000555"
     CDS             complement(3346..3657)
                     /locus_tag="IW797_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1223351.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(3659..3856)
                     /locus_tag="IW797_000556"
     CDS             complement(3659..3856)
                     /locus_tag="IW797_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ1223352.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(4250..5977)
                     /locus_tag="IW797_000557"
     CDS             complement(4250..5977)
                     /locus_tag="IW797_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1223353.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(5970..7163)
                     /locus_tag="IW797_000558"
     CDS             complement(5970..7163)
                     /locus_tag="IW797_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1223354.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(7350..7994)
                     /locus_tag="IW797_000559"
     CDS             complement(7350..7994)
                     /locus_tag="IW797_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1223355.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(8088..8222)
                     /locus_tag="IW797_000560"
     CDS             complement(8088..8222)
                     /locus_tag="IW797_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ1223356.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(8224..10356)
                     /gene="feoB"
                     /locus_tag="IW797_000561"
     CDS             complement(8224..10356)
                     /gene="feoB"
                     /locus_tag="IW797_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ1223357.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(10353..10826)
                     /locus_tag="IW797_000562"
     CDS             complement(10353..10826)
                     /locus_tag="IW797_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ1223358.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            11227..11451
                     /gene="nrdH"
                     /locus_tag="IW797_000563"
     CDS             11227..11451
                     /gene="nrdH"
                     /locus_tag="IW797_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ1223359.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            11610..13769
                     /gene="nrdE"
                     /locus_tag="IW797_000564"
     CDS             11610..13769
                     /gene="nrdE"
                     /locus_tag="IW797_000564"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ1223360.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKAIYYVRTFTDDAEEIGSNQCESCVI"
     gene            13819..14784
                     /gene="nrdF"
                     /locus_tag="IW797_000565"
     CDS             13819..14784
                     /gene="nrdF"
                     /locus_tag="IW797_000565"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ1223361.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(14873..16012)
                     /locus_tag="IW797_000566"
     CDS             complement(14873..16012)
                     /locus_tag="IW797_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1223362.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(16321..17034)
                     /locus_tag="IW797_000567"
     CDS             complement(16321..17034)
                     /locus_tag="IW797_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ1223363.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(17288..17989)
                     /gene="nagB"
                     /locus_tag="IW797_000568"
     CDS             complement(17288..17989)
                     /gene="nagB"
                     /locus_tag="IW797_000568"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ1223364.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            18223..19755
                     /locus_tag="IW797_000569"
     CDS             18223..19755
                     /locus_tag="IW797_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1223365.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(19946..21148)
                     /locus_tag="IW797_000570"
     CDS             complement(19946..21148)
                     /locus_tag="IW797_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1223366.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(21230..21919)
                     /locus_tag="IW797_000571"
     CDS             complement(21230..21919)
                     /locus_tag="IW797_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1223367.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(22025..22393)
                     /gene="rplL"
                     /locus_tag="IW797_000572"
     CDS             complement(22025..22393)
                     /gene="rplL"
                     /locus_tag="IW797_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ1223368.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(22446..22949)
                     /locus_tag="IW797_000573"
     CDS             complement(22446..22949)
                     /locus_tag="IW797_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ1223369.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(23359..24048)
                     /gene="rplA"
                     /locus_tag="IW797_000574"
     CDS             complement(23359..24048)
                     /gene="rplA"
                     /locus_tag="IW797_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ1223370.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(24152..24574)
                     /gene="rplK"
                     /locus_tag="IW797_000575"
     CDS             complement(24152..24574)
                     /gene="rplK"
                     /locus_tag="IW797_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ1223371.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            24917..25585
                     /gene="sdaAB"
                     /locus_tag="IW797_000576"
     CDS             24917..25585
                     /gene="sdaAB"
                     /locus_tag="IW797_000576"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ1223372.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            25598..26470
                     /gene="sdaAA"
                     /locus_tag="IW797_000577"
     CDS             25598..26470
                     /gene="sdaAA"
                     /locus_tag="IW797_000577"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ1223373.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(26561..27580)
                     /locus_tag="IW797_000578"
     CDS             complement(26561..27580)
                     /locus_tag="IW797_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ1223374.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(27753..28640)
                     /locus_tag="IW797_000579"
     CDS             complement(27753..28640)
                     /locus_tag="IW797_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1223375.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(28717..29262)
                     /gene="nusG"
                     /locus_tag="IW797_000580"
     CDS             complement(28717..29262)
                     /gene="nusG"
                     /locus_tag="IW797_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ1223376.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(29363..29533)
                     /gene="secE"
                     /locus_tag="IW797_000581"
     CDS             complement(29363..29533)
                     /gene="secE"
                     /locus_tag="IW797_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ1223377.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(29557..29709)
                     /gene="rpmG"
                     /locus_tag="IW797_000582"
     CDS             complement(29557..29709)
                     /gene="rpmG"
                     /locus_tag="IW797_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1223378.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            29871..30515
                     /locus_tag="IW797_000583"
     CDS             29871..30515
                     /locus_tag="IW797_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1223379.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(30571..31074)
                     /locus_tag="IW797_000584"
     CDS             complement(30571..31074)
                     /locus_tag="IW797_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1223380.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(31087..31133)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            31671..32636
                     /locus_tag="IW797_000585"
     CDS             31671..32636
                     /locus_tag="IW797_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ1223381.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(32735..33907)
                     /locus_tag="IW797_000586"
     CDS             complement(32735..33907)
                     /locus_tag="IW797_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1223382.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(33894..35039)
                     /locus_tag="IW797_000587"
     CDS             complement(33894..35039)
                     /locus_tag="IW797_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1223383.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(35053..35977)
                     /locus_tag="IW797_000588"
                     /pseudo
     CDS             complement(35053..35977)
                     /locus_tag="IW797_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            36094..37173
                     /locus_tag="IW797_000589"
     CDS             36094..37173
                     /locus_tag="IW797_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1223384.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            37205..37798
                     /locus_tag="IW797_000590"
     CDS             37205..37798
                     /locus_tag="IW797_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1223385.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(37908..38135)
                     /locus_tag="IW797_000591"
     CDS             complement(37908..38135)
                     /locus_tag="IW797_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1223386.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(38238..40826)
                     /locus_tag="IW797_000592"
     CDS             complement(38238..40826)
                     /locus_tag="IW797_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ1223387.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(40846..41505)
                     /locus_tag="IW797_000593"
     CDS             complement(40846..41505)
                     /locus_tag="IW797_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1223388.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(41554..42057)
                     /gene="trmL"
                     /locus_tag="IW797_000594"
     CDS             complement(41554..42057)
                     /gene="trmL"
                     /locus_tag="IW797_000594"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ1223389.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(42278..43144)
                     /locus_tag="IW797_000595"
     CDS             complement(42278..43144)
                     /locus_tag="IW797_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1223390.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            43253..43894
                     /locus_tag="IW797_000596"
     CDS             43253..43894
                     /locus_tag="IW797_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ1223391.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(43956..45317)
                     /gene="mgtE"
                     /locus_tag="IW797_000597"
     CDS             complement(43956..45317)
                     /gene="mgtE"
                     /locus_tag="IW797_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ1223392.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(45335..46234)
                     /locus_tag="IW797_000598"
     CDS             complement(45335..46234)
                     /locus_tag="IW797_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1223393.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(46236..47033)
                     /locus_tag="IW797_000599"
     CDS             complement(46236..47033)
                     /locus_tag="IW797_000599"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ1223394.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(47011..47715)
                     /locus_tag="IW797_000600"
     CDS             complement(47011..47715)
                     /locus_tag="IW797_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ1223395.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTHLK
                     KESEREEAGDESSDRS"
     gene            47846..48421
                     /locus_tag="IW797_000601"
     CDS             47846..48421
                     /locus_tag="IW797_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ1223396.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            48539..49216
                     /locus_tag="IW797_000602"
     CDS             48539..49216
                     /locus_tag="IW797_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1223397.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(49295..51103)
                     /gene="pepF"
                     /locus_tag="IW797_000603"
     CDS             complement(49295..51103)
                     /gene="pepF"
                     /locus_tag="IW797_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ1223398.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(51279..52277)
                     /locus_tag="IW797_000604"
     CDS             complement(51279..52277)
                     /locus_tag="IW797_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ1223399.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(52503..53156)
                     /locus_tag="IW797_000605"
     CDS             complement(52503..53156)
                     /locus_tag="IW797_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ1223400.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        16152 a        11104 c         9275 g        16931 t
ORIGIN      
        1 attaaaacca tgtgaaactg cctcacggca gcttacaatt actttttcat cttcacccgt
       61 taagtgatga aaaaagaacc cctttctgct atggttaaag tgactaaacc aaccaaaaga
      121 aaggagttct tttcatgact agcttacaca aaaaccaagt aaaattcaat tcaaatatca
      181 ctatttctca tacaggtggt caattatcga gtgattcggg tctcgtccta gtgaaagagc
      241 tgatgaacat ctttggtttt tctgaactgg ctaagcaaca cattcacatt gaagacaaac
      301 gagcgtattt tactcatgac aacttatcca tacttgagca gttcattatg caattaattg
      361 ctggttactc agctgattct gcagctaatc tcctgagaca agatcctgtg tttaaagctg
      421 ttttagatag gaaagaactc gcttctcaat cttcactttc ccgattttta gatcgactat
      481 ctgaggagaa tatccatgaa cttcaagctt tgaaccaaga acttatcgat aaagcacgcc
      541 tcatccgtaa tgatacggaa ttaatcattg atttagattc gacccattca gatacatttg
      601 gtcaccaaga acaaacgaat tataatacac actaccaaac ctatggttac catccattgg
      661 tagcttttga cggattgact ggtgacttct taaaagctga actacgttca ggaaatcaat
      721 atacatcaaa aggcgtaaag gagtttctca cacctttatt agagcactat aatcactctc
      781 taccaaacac tgacatcttg gttcgtggag acagcgggtt cgctacacct ggtgtgtatg
      841 attcgtgtga atcaaaaaag agtcattatg ttattcgact gaagaataat cgtagactag
      901 gtcaaatagc tgagaaatca gtactttatg gcgataatca aaagtgggaa gaacgagaag
      961 ttcagtactt ctcgaccact tatcaagcac aatcctggtc acaaagtcgt cgcgtgtgta
     1021 tacgctcaac acgtgaagcg ggcgaactac tctttcgaca tgaatttatc gtgacgaatc
     1081 tatcagaaaa tgtttctcct gataccatct tttctatcta tgccaaacgt ggcacaatgg
     1141 agaacttcat taaagaagcg aaagctggct tttactttga caaaacagac agtcctcgct
     1201 ttttggagaa tcatgtccga atgatgctca gcttgatagc ttacaactta gtcaacttct
     1261 taagaacgat tggctttgaa gaagtccaaa agggaatgac cattcattct attcgattga
     1321 agtttctgaa agttgctggg aaattagtcc aaacgggtag acgagtctat ctcaaattat
     1381 ctagttatca tgtgtatcag aatgaattct acagggtctt tgctcgcctg aggcgagcca
     1441 gtcaatggat ctaatccaac aatctaacga tttttttact gggaagttat ccagggagaa
     1501 gtatgctcaa aatgccttac acccaaaatt tattcctcaa tatttttgaa cagtgaatgt
     1561 ttgaagtaac tttttagtca aaatcagttg atagggatat tagtgataca tatttcaata
     1621 aaatgtgcca aagaattaaa gctatgaatt attcaggtta aatactttat cgtgaaaaag
     1681 aatgaaatta tcaataattc gttctaactg atttaattgt ataatttgat cttcacataa
     1741 tattattgga taactcataa atcctccttc cctcaaaaca acaaaataac ttgagtagta
     1801 aattgatacg gatcgttttg atagttcaca aacatattgg gattcttttt attgatttcc
     1861 tctatactag ataaacctaa cccttgatgt cccttttttg ttgttattcc ttttttcatt
     1921 agagtatcta tagagagtag tatctctcta caactattcg atatcgaaat ttctatctgt
     1981 ttatcatctc ggtaaatagc tagagacagt tctctatctt gactctctat agatgcttca
     2041 attgcattat cgagaataat tccaagcacc ctaatacagt caaatattgg aacaggaact
     2101 tcaataatag tacttggaca ttcgaattta caacaaatct tattgttatt tgcttggtat
     2161 agcttcgcta tcaacaaact tttcaaatat tcatttttta tattttttaa ataacgatat
     2221 tcaaacttat tggttagata agaattagaa tagatctcta attcttttat ttctttttcc
     2281 aatatcgaat catggttttg attagcgatt tcttttaaac tcagcaataa atttttatag
     2341 tcatgtctaa atctggcaaa tttttcttga tctttttcca gtgaatcagt atattttttt
     2401 agataaacca attcttttct tttcaattgc tgttcatatt gttctcgttg cctaatagtt
     2461 attctaataa ataaaaatag aacaacaaac atttgaatga ttaaaaatat taagacacca
     2521 aacacaaaat gatcaaatac ttgatattga tgagcaacat aagaaacaaa taaaccaatt
     2581 gtaagtaaat aaatcatgaa tatagcagta agtttagaac tggactgttc aacaatccta
     2641 tttatagcta attttctata taaccaaata aacatcagca aaaacaaaga atcaagcatt
     2701 atctggaata ctacataacc ataccctttt atcccttcaa atgaaaacat atacgtcata
     2761 accatggaag taataataga tctgaaatca tcaataagca tacataatag taatttattt
     2821 aaaagcacta tattagtttt cttcttttcc tttaatataa aataacttcc gattataggt
     2881 atagctgcgg aaacatcagt aaataattca acaaaaaaag tgagtggtat caataaacat
     2941 acaaaaagat attttctttt gttaagatca gtattagtca tataaaataa gagaaaaaag
     3001 aaaaagtttc ccacgaacat tcttaataga taagcttttg tatcgatagt aatcaagaga
     3061 tcccctcctg attttaggat atcataaaaa aaattttagt tacacttttt aaataatgaa
     3121 aaaacacatt ccactaaatt tgatgccggt tttccttgag gttttgttcc agcacctcct
     3181 ttaatttttt taagctcttg atctgaacat tgtttagcat ttagtctatt tttttcttcc
     3241 attacgattc cccctacaaa ctataaatac ttttatgtca ctgattcaat atacctattt
     3301 ttctaatcaa aaaattttga gacatattta atattataag tatcattaaa attgagattt
     3361 atctccataa tctgctcgat ataaaccatt tattccagca gttcttccaa tttcactcaa
     3421 atttcttatt aattcctctt gatattcagt gaatcttatt ttattagtat aagcagtaaa
     3481 atatagataa ttcaccaatc tatttattaa aggaccggga tctaattcat atttttgttt
     3541 ttctaatttt aaatatacct tttgtaaaac ctcaagaata tcagaatatt taggatcttt
     3601 acttatagat atatcattat ataattcatg aacaatttgc ttagcatttt ttttcatttt
     3661 agcacttccc tggaattgct ccacctaaaa aaccacctat agacattcct gcaatacaag
     3721 tagttgcctt ggcccaatcg accgtacatt tatttttagt gcaatacact ccatttccat
     3781 aatattttcc actatgagtg gtacccccat agataagttg tgtctcttta atagacaaaa
     3841 tttttaaatg tttcataatc taatcacccc taaattaatg tttttagact attattctaa
     3901 cattcattaa atttcaaaaa aaaattcctt cttgaaacgt agcaaaataa ttcctgaata
     3961 agaatttaat cacttttaag tatataaact atgtatattc tttaatattt tttgagaatg
     4021 cacttcgtac ataataaact ttaatcacat tagtctttca tacctacgat cgatctcttt
     4081 gactcgcacc cataaattca taatagtttg tcggcgtctt tggacgtttc gaagatgagc
     4141 tacgcattct ctttttttat agacaaaaaa gatagagacg aacatggagc ttccaaattt
     4201 ggatatttga tccaattttg gaagctccat ttatttcgtg tctgtctttt tattttttcc
     4261 agctttcgac taattcttgg ttctctttga tccagtcttt tgctgcttgc tgtggatctt
     4321 ttccattgtt gatttccaac ataacttttt ccatatcttt ttctgaccaa tggaaattgt
     4381 ctaatacttt gtaagcttct ggctggtctt ctttcaatcc ttttcgaacc atcgtatgga
     4441 tctgctcttc gcttcccatt ccgttttcag gatcttctaa gtatttcaga tcatatttgg
     4501 caaacatcca atgaggtgtc catcctgtaa ctacaattgg ttcgtgtttt ttgatcgctt
     4561 gacctagtgc aacggtcatt gctcctgaag aagatgtctc tactttccag tctttcaagt
     4621 tgtcatattt ttggatcgtg ttttcagcag ccgtaactac accagctcct ggctcgattc
     4681 ctatgatttt cttacctgcc tgatcggtta actcatcgat cgaatttaca tccatgtagg
     4741 ctggcactgc taaaccaact tttgctcctg tcaaattggc acctaggtct tcgacttgat
     4801 ctttatattg tgcatattgt gaaccatgag ttttaggtag ccaagctgaa accatggcat
     4861 ctgactcgcc atttgaaaca gatttccaca tgattgaatt atctaatggc gtcatcgtta
     4921 catcatagcc catttgtttc aatacttcac cgactacatt tgttgaagca acttctgtat
     4981 cccattcaac ataagataag ttgatacgtt ttgtttcttt ggcagatgaa aaactcccta
     5041 taccaatcgc acctaagatc actactacta cagcaccaat gatcagtcct tgacgttttt
     5101 tacttttctg tgttgttgct cctgcagctt ttttattgtt tggctggttc aaatgctgtg
     5161 taaaacggtc aatgatgatt gctaagataa ctaatgcaac cccgttgaca aatccattcc
     5221 cgacttgtgc tctttgcaat gctgataata cgccgcgtcc taagccgggt gcaccgatca
     5281 tagaggcaat aacgaccatt gaaagtgaca gcattgttgt ttgattgatc cctgccatga
     5341 tcgtactttt agctaaaggc aattctagtt tgaatagttt ttgacgactt gtgctcccga
     5401 atgaatcaga agcttctact aattctttag gaacttgtcg aatccctaaa ttcgtaaaac
     5461 gaacggttgg tggcagagca aagatgactg aggcaaatac ccctggaacc atcccgatac
     5521 cgaaaaatgc aactgctgga atcaaataaa cgaatcctgg cattgtctgc ataaagtcaa
     5581 ggatcggttt gataatactt tgggctttac tgctttttgc catcaagatc cctaatggta
     5641 ccccaatgat gatcgagatc acactagata aaagaacaag tgtgacagta ttcatcaaat
     5701 cattccatag accttggtta taaataaacc atagaccgat taatgtgaac aacgtaagtc
     5761 ctggacgttt cttagaaata aagaaagctg ctaccgtcaa taacaagata aacaaaggag
     5821 ctggaatcac taacagtaaa tttgtaatcc ccgacatcac tgcctgtcca atcgtttgta
     5881 aaaatgaaaa taggccagaa aaagttgtcg taaaccattc tgtgatattt tctacccagt
     5941 ttgccactgg taattgatag ttcaatagat tattcatgtt cactcacctc gcttgtttct
     6001 gctaatgctt caatcacact acctcggatg acgacacctt ttaattttcc atcctctaca
     6061 actgcaagtg gagtcggtga gtcataaata atattgaaaa tatcacttac caccatgtct
     6121 ttcccaatca ccgtcacatt ttcatctagg aagtcgatca atggacgttt ttgcttccta
     6181 gcttctaacg cttgatctgc agtgatgatc cctttcaggt gtctctttct gtcaacagcc
     6241 atcagcatgc tgacttcttc attacgcatc cttgtcaatg caacgtttgg tccatcagat
     6301 tcgatattcg tcgtcaatgc aggtaccatg atgttttgag ccgtaagcac tttggaacga
     6361 tcgacttctt ccacaaattc acgtacataa tcatttgctg gattggtcaa tatttcttca
     6421 cctgttccga tctgcatgat ttcgccgtct ttcatcaagg cgattctgtc cccaatccgt
     6481 aaagcttcat tcaaatcatg ggtaatgaaa ataatcgttt tttgaacatt ggcttgcagg
     6541 tccagtaatt cgtcttgcat ttctcgccgg atcagcggat caagagccga gaacgcttcg
     6601 tccatcagca agatctcagg atcatttgct aaagctctgg ctaggccaac ccgttgttgc
     6661 atcccaccag ataactgact aggatattgg tctttaaatg aaagcaaact agaattttct
     6721 aatgcttttt ctgctttttc ttgtcgttct tctttaggta caccacggac ttctaacccg
     6781 tattctgtat tttccaagat cgttcgatgt ggaaacagac cgaagttttg gaaaaccata
     6841 ttgattttat gacgacggac ttctcgcaat tcttctttgc ttaacttcga tacatcttct
     6901 ccatcaatat agatactgcc cgaagttggc tcaatcagac gattcaatag acggatcaat
     6961 gtggatttcc cactcccgga aagccccatg atcacgaaga tttctccttc ttttacgtca
     7021 aaattcacgt catacacacc aaccgttgca ccggttttct ttaaaatctc cgttttcggc
     7081 tggtgatcat tcatcatatc taaagcggct tgtgtctttt taccaaatat ctttgttaaa
     7141 tgttttactt ctacttttac caaacaaatt ctcctttttt atgtaaattc atttttcttt
     7201 ctgaaaaaag tgaccacact atgttaacat catgtagtca ctttgtcaaa aatgaaagaa
     7261 atttgcttgt ccaaactgaa aaaaacttgg ctaaatcttt gtaaataaaa ggtttagcca
     7321 agttaatatt tttttaatat ttcacacaat cattctgcaa gctgatgctc cgggtaaaaa
     7381 aagtttgtca tacgctgtag tgtatattca tttccaataa aatgaacgat gtcccctgcc
     7441 gtaaatttag cataggggcc aggtgaaaca agcagttctt cattatgaga aattgcgata
     7501 actgtggcgc cagtcgcctg ccagatattc aactcgctaa ttgaacggtc cagatgcatt
     7561 gctttttctg ataaagataa ttcaaacgga agtaaaggat tcatcttatg tattcgcttc
     7621 gtttgattga ccagctgatc caataattga gaaaaatgat tcagctcatc ttgttgcttc
     7681 tccacacttg ccatgatttc tgcttttgta tcttggatgg attgtacatc ttgatattgc
     7741 aataggaaat cttttgcttt ttccttcgaa gcaacaaatg ccccacttcc atgccgtact
     7801 tccatgattc caagatctac cagcacattg atagccttgc gggtagtctc aggagaagca
     7861 ttgtacgtac tggcaagtgt tgagcgggca tggatcttct cacccacttt atatcggttt
     7921 tcaacgatac gttcggctaa atcaacagca atctgctggt agcgcgggcg gacgattttt
     7981 ttctttccag acattttctt tcctctttct ttttttactt tccctcatct tactatgctc
     8041 tagcaaaata agcaaaaaag gggagaatag cctcttgcaa cgactgatca tcgcttgatc
     8101 tgttcctttt ttactgggca agctgtttga cagtcatcac aattcttacc ggattttaaa
     8161 cgagtataga caattgcgcc tgcacttcca aagattaaca cacttaataa gaatgtcgcc
     8221 atcctattcc tctccttcta atgacaatat agtataaaac tggttcttgg attttggctg
     8281 acggaacaaa acataaacga gtcctatcat cagtaaaata gcaagatacg tccctaacgc
     8341 tacctgtcca ccctccaata agtgtcccaa ctgataaatc acaaaactaa ctaaataagc
     8401 taaaccgcat tgatagccta tggcgatcca agtccatttc ttggcattca tttcccgatg
     8461 gatagcccca attgccgcaa aacaaggcgc acaaagtaaa ttgaaggcta acaatgaata
     8521 agctgctgcg ggagtatatg tgtgttgtag cacttgccag atttctctgc cattttcgga
     8581 tacttccgca tgaccaaaca aaataccaaa tgtgccaata atattttctt tagcgaccaa
     8641 tcctgtaatc gctgcgactg ttccttgcca gtttccccaa ccaagcggct gaaaaacaac
     8701 tgccaggatt cgcccgaaaa aggctaagat actttggtct tcagaaacag gttgagcata
     8761 aaaattataa gaagagctga accagatcac gatactagaa acaaagataa tcgttcctgc
     8821 ttttttaaca aaagacttgc tgcggtcata ggtttgtctt aggaccgctg accattgagg
     8881 aaaatgatat gcgggcaatt ccatgataaa aggagctgta tctccagcaa acaaacgtgt
     8941 ttttttcaag ctgattccag agaaaataat tgcagtcata ccaagaaaat aggcagaagg
     9001 agctacccaa gattgatgag gaaaaaaggc ccctgctact aaggcaataa tcggcaactt
     9061 tgcagaacaa ggcatgaagg ttgtgaccat gatcgtcaaa cgacgatctt gctcgttttc
     9121 aatcgtgcga ctggccatga cgcctggcac tccacatcct gtagcaatca gcataggaat
     9181 gaaggatttt cctgaaagac cgaatttcct gaaaagtcga tccatgacaa aagcgatcct
     9241 cgccatatat ccgcagtctt ccagaaagct caagcataaa aacaatataa tcaattgcgg
     9301 tagaaatcct aacactgctc ccacgcctgc gatgatgccg ttcaagatca gctcctgcat
     9361 ccatcccgct acttgccatt cagctaacaa tctgccaaca tgttctggta accatgtacc
     9421 aaacaatgtg tcattgatcc aatctgtccc cattgttcct actgtttgaa tagccagata
     9481 atagatcagc cacatcacaa atgcgaagat cggcaaggct aaccaacgat tcgttgcaat
     9541 ctgatcgatt ttgtcactca tcgataattg gaaagaatca tttgacacaa cacagagtgt
     9601 acatagttgg gtaataaatt cataacgttc attgaccaga atcgtctcac tatcgtcgtc
     9661 caatagtttc tctgttaact ggatcagttg ctcgatctct ttttgctgag aaactgctat
     9721 ctcgtaatcc tctcttacct tctgatctcg ttcaaaatat ttcattgcat accagcgttt
     9781 cttgccgctt tgttctccta gtacatcttc aatttcatgt aaagctgctt ctaaacgtgg
     9841 atcatatgta cagggaacag gaatagaagg ccgctttttt gcagactcct gaatcttttc
     9901 gattagtttt tcgattcctt gctttttcaa tacgctgatt ggtaaaactg gtacaccaag
     9961 accataggaa agcttttcta gattgatttt ttttccttct tttttgatga catccatcat
    10021 attcagacca atgatcatag gaatgtctaa atccaataac tgcgtagtta aataaagatt
    10081 tcgttctaaa ttggtagcat caataatgtt gatgatcagt tcaggctgtt cctgaaccaa
    10141 ataatctctt gcaatcactt cttctggtgt ataaggagac aaagaataga tgccgggtag
    10201 gtcctgaata tgaatgttcg gatttttttt caacgatcct tctttttttt ctactgttac
    10261 gcccggccaa ttccctacat attgattcga accagtcaag agattgaaca agctagtttt
    10321 cccgctgttt ggatttccga ctaacgcgat attcattctt tttcctccct ttgtatcaag
    10381 attagttcgg cctctgactt tcgtaacgtc agttcatacc ctcgcactgt cagttcgatt
    10441 ggatctccca gaggggctag ttttcggata actattttta catttcgcgt caatcccata
    10501 tccattaacc gtcgcttcac tgcgccttgt ccgtggattg ctacgacgat cccagtctct
    10561 cctgctttta attgatcaag agaagtccag ctttcagagg aagcagtctt tttattgaca
    10621 aagatttgac ctagtatttc ccgacttact gccactcgat gactttttac cagtaaaatg
    10681 gcattgttgc ctgataaatt gacgacgacg attttacttc cggcaacgat cccgatattt
    10741 tgcaaatggc gttttgtttt ttcttctgtt tcgattcgtt cgacttgata aacgaaattc
    10801 tgctttgcct gcgctaaggt ctgcatattg attcctctct tgtgaaataa atcatgtatt
    10861 aaaatcattc attatatgtt ctaattttct tacgtgttac gcaaataaat gagaatcatt
    10921 ctcacataga atactctcgg taattgagaa tgtcaattat acttgtgata ttatgagcga
    10981 atctttagta ttgatagata aaaatgagaa aaaaatattt cttcatagtt ttccacaatt
    11041 ttttctctat taaatttgct attttcccac acgatacttg ttttcagctc ttttttcaaa
    11101 aacacaaaac ttagtattcc acagtttcca caacacaata tgtagtgttt gttgtttgac
    11161 ttttttacta agagttttat actatctatt atagataata agaatgtgtt agaggaggca
    11221 ctcaccatga acgtaaaaat cttttctaaa aataattgta tgcaatgtaa gatggtcaaa
    11281 cggtttttaa cagaaaatca aattgctttt gaagaagtca atatcgatga acagcctgaa
    11341 gcagtagact ggttgaaagc acaaggattc caaagtgttc ctgtcattac ttctgatgca
    11401 gaaacagtta tcggattccg tcctgatcag ttgcgtaaac ttgccagcta acatgactaa
    11461 aagaatcaaa acgtgataca tggatttgaa gggcttgaga cagcaccagt ttttgtcgca
    11521 aacttccaaa tcctttttta aagcttaaaa acaatgcaat gatttgcaat caagcatcat
    11581 caaatacgaa atgaaggttg ggcgtacgta tgagtcttaa aaatttacaa gatgtcagtt
    11641 atttcaagct gaataatgaa atcaatcggc cagtcaatgg tcaaatccct ttgaataagg
    11701 ataaagaagc tttagttgcc ttttttgagg aaaatgtcaa accaaatacg atgacttttc
    11761 tatctattat ggaaaaaatc aattacttga tcgatcaaga atatatagaa gcagaattca
    11821 ttcaattgta ttcaccatca tttattgaaa agctatatca atttttggac gagcaacatt
    11881 tttctttcaa atcttttatg gctgcttaca aattttattc tcaatatgcg ttgaaaacca
    11941 acgacggttc cgcttatctg gaaacttatg aagatcgagt agccttcaat gcgctttact
    12001 ttgcgaacgg ggatgaagaa ttagcacttg ctcttgcaga cgaaatgatc catcaacgat
    12061 atcagccagc tacaccttct ttcttgaatg ctggacgtaa acgtcggggc gaattggtgt
    12121 cttgtttctt gactcaagtc actgacgata tgaactctat cgggcgctca atcaactctg
    12181 ctttgcaatt gtcccgtatt ggtggcggcg tagggattac tttatcaaat cttcgtgaag
    12241 ccggcgctcc tatcaaagga tatgagggtg ctgcaagtgg tgtcgttcca gtcatgaaat
    12301 tattcgaaga tagtttcagc tattctaatc aattaggtca gcgtcaagga gctggcgtgg
    12361 tttatttgaa cgttttccat ccagacatta ttgctttcct ttcagcaaaa aaagaaaatg
    12421 cagatgaaaa gattcgcgta aaaactcttt ctcttggcgt gatcgtacct gataaatttt
    12481 atgaattgac acgtaaaaac gaagatatgt atttatttag tccatatagt gtcgaaaaag
    12541 aatatggtgt cccattctct tatgtaaata ttacagctga gtatgatcat ctagttgcaa
    12601 atccaaacat tcgcaaacaa aaaatcaaag cgcgtgatct tgaaaatgaa atctcaaaat
    12661 tacaacaaga atctggctat ccttacgtaa tcaatattga tacagcgaac cgcgagaatc
    12721 caatcgatgg caagatcatc atgagtaatt tatgttcaga gattttacaa gttcaaacgc
    12781 cttctgtgat taacggcaaa caggaatatg aagtattagg aacggatatc tcatgtaact
    12841 taggctcgac aaatatcgtg aatttgatgc aaagccctaa ctttggtcaa tctgtgcgta
    12901 cgatgactcg tgcattgaca tttattacag atgcatctga tatcgatgtc gtaccaccga
    12961 tccaaaacgg aaataagctg aatcatacga ttggattagg tgccatgggc ttacacactt
    13021 atcttgcaaa agaacaaatg gaatatggct cagaggattc attagacttt acaaatattt
    13081 acttcatgct gttgaattat tggacacttg ttgaaagcaa caatatcgct cgtgaaagaa
    13141 aacaagtttt ccataatttt gaaaaatcag cttatgctga cggcacttat ttcgaaaaat
    13201 acgtaactgg agaattccag ccaaaatcag aaaaagtcaa agcattgttt gaaggcattt
    13261 ttatcccaac tgctgaagat tggaatgctt tgaaacaagc agtaatgaaa gatggactgt
    13321 atcatcaaaa tcgcttggca gtagcaccaa atggctcaat ttcatatatc aacgatacga
    13381 gtgcaagtat tcatcctatc actcgcatga tcgaagaacg ccaagagaag aaaatcggta
    13441 aaatttatta cccagcgcct tatctatcca atgagacgat tccgtattac acttctgctt
    13501 atgatatgga catgcgtaaa gtaatcgatg tttatgcaat ggcacaacaa cacgtcgatc
    13561 aaggcatgag tttgacttta ttcatgcgtt ctgaaatccc agaaggcatt tatgaatgga
    13621 agacaacaac gaagcaaacg acacgtgatt tgaatatttt aagacattac gctttccaca
    13681 aaggaatcaa agccatttat tacgtacgta cctttactga tgatgcagaa gaaatcggaa
    13741 gcaatcaatg tgaaagctgc gtgatctaat atcatagata gattcgctta ttcatatttt
    13801 aattaaagga gcattctcat gtcagaaaca tattatgaag caataaactg gaatgaaatt
    13861 gaagatatca ttgataaatc cacttgggag aagctgacag agcaattttg gctagataca
    13921 cgtatcccac tttcaaatga cctagatgat tggcgtacat tgtctcaatt ggaaaaagat
    13981 acagtaggac atgtatttgg cggtttgaca ctgctagata ctgtccaatc tgaaagtggc
    14041 atggatcagt tgcgtaaaga tgtccgcact ccccatgaag aagccgtttt gaacaatatt
    14101 caattcatgg aatccgttca tgccaaaagt tattcttcta tttttagtac actgaatacg
    14161 aaaaaagaaa tcgaagaaat ttttgactgg acaaatacga atccttatct gcaaaaaaaa
    14221 gctgagcgta tcaatgaaat ctacaaacac ggtacaccgc tagaaaagaa aattgccagc
    14281 gtctttttag aaacattctt gttctattca ggtttttata ctcctcttta ttatttagga
    14341 aacaacaaat tggctaacgt agcagagatc atcaaactaa tcatccgaga tgaatctgta
    14401 catggaacct acatcggtta caaattccaa ttaggattca acgagttacc tgaagcagaa
    14461 caagaaacgt taaaagactg gatgtacaat cttctttatg agctttacga aaacgaagaa
    14521 cgttatacag aagaattata cgatccaatc ggctggacag aagaagtcaa gacattccta
    14581 cgctataatg ccaacaaagc attgatgaac ctagggatgg atccactctt tccagatact
    14641 gccaatgatg tcaacccaat cgtaatgaac gggatttcaa caggcacaag caaccatgac
    14701 ttcttctcac aagtcggaaa cggctatttg ttaggtcatg tagaagcgat gaaagacgac
    14761 gattatttga ttggattgga ataaggattt attttaattc aaaaattttt cgaaaaataa
    14821 aaagagttta gcgtacagat attttaaatg tacactgaac tcttttttat gcttattcac
    14881 ttgtttcttc cagcactttt ggactgtcta acttttccga aacatgttcc attcttctca
    14941 ataaatagat tttccggata tttaatatta tcgtcttaat cactgcatag acaggtatgc
    15001 aaatcagcat gcctaatatt cctgcgacac ttcctgctcc aatcaatacc aagatgatcg
    15061 tcaaaggatg gatattcatt gatttcccaa ataacaacgg cttgatcaaa tttccatcta
    15121 attgttggat cactaaaatc gacaacgcca tatacaaagc ttgaaatgga gagataaaca
    15181 aaccgacaat gattgctggt accgctccaa taaaagggcc gacatacggg ataatattcg
    15241 tgattccgca aatgaccgca agcaacaagc tataaggctg atcgaatatt ctcattgcca
    15301 aaaagctcat tacaccaata atcaaagcat ctaagatcgt actgctgata taggcagata
    15361 atgtttcgtt taattctttt accgtttggc gtaactcttt acgaatcgct ttcggaaaga
    15421 agtgagacaa cgcatcaata aatttgtgcc catccttgaa cataaatagc aaaataaatg
    15481 gaacggtaaa taacaaaata aaaaattgca tcataaatga aatgattttt gagacacttg
    15541 cagataaact gactaatatg atattgaaca aattactaag agataaattt gctgatgcta
    15601 attgttcttc caaattgaaa tttttaaact cttcccgctg cgccaattca tttaaccatt
    15661 tgcttgtttc atctgcatac attggcaagc tctgggtcaa ttgaattgtc tgttccatta
    15721 actgaggcac tacattcatg acagttaaaa caactaaaat gattacgacc aaaaacgtca
    15781 ataaaaaacc gaacactctc gggatccgat gcttttcgag aaaaacaacg attggatcga
    15841 acatataata gataaaacca gcaacaagca atggtaaaaa aactgccgaa agcattttca
    15901 atattggact aaaaatattc ggcatttgta aaataaagta cacgccgatg atcgtgagta
    15961 ctagctccac tgtccagaaa aataattttg attgtttaaa acgtgattgc atacatctcc
    16021 tccaaggtcc tttgatactt ctattctatc atttaacaaa gagttacgcg ctgagatttt
    16081 catttgctta aaaaaaacaa aaaaaataaa catattttaa tgagtagacg agtacttaaa
    16141 caaaaacacg gtttggagta gtgacaaaaa ttatactgca gtcccttttt ccaaataaac
    16201 ggtgttcaaa agcttgcatc ttcgtaataa gcccagccaa acgaaaaata taaggaacta
    16261 tttttatttg gctgggctta ttacttgatg taggacatct attttacaac ctcagatcat
    16321 ttattgcgca atactgacta aatgcaactt tgtgatattt ttgtgtacgg acaactcctc
    16381 gatcacatcg gcataagtga gcccttttgg aagatcaatc gtatagatat ttttgtacac
    16441 tcggtagtca tcaacgaatt ttacgtcaaa attcacatct tcgattacga tatttttctc
    16501 ttcaaaataa tcggtaagaa aatctttggt ttcttttcta tggataaatt ggatttctaa
    16561 ctttttagtc gtaggtacat ggatcacgcg tttcactact gtgagtgcaa tagcaatccc
    16621 gataaaactg atgatcgcaa tggaatagta acccatgccg atggcgatcc ctaatccagc
    16681 aattgcccat aaagaagcgg cagtcgtaag tcccgtcacc gattgtttcg tgacgatgat
    16741 cgttcccgcg cctaagaaac cgacaccact caccacttga gcaatcagcc ttgcttcatc
    16801 tgaacgaatc gttccagcaa gctgtggata ctctaaagca tctcttaatg caccactagc
    16861 gatctcaact tggatcaatg caatgataca tgctcccata caaactaaaa tatgcgtacg
    16921 catacctgct ggacgatttt tatattgtcg ttcaaaaccg attgctccgc caaatagcat
    16981 agcgatccct aaacgtaaaa taatctctgg aattgtcaat accatatcat tcatctaaac
    17041 accctccagt ccaatatatc atgtctgaaa ggagcagaca aaaaaagtgc aatttttacc
    17101 tcttacgaaa aaaggactgt gacaaaattt ttgtcatagt cctttttatg aataaacggt
    17161 gttcaatcag ctgaccctcg gtattactca acaaacggca aaatatgggg agctattttt
    17221 cctttgtctt ctctaatact caggtcaaaa cgctgatttc acaacctctt tagcctacta
    17281 ttgaaaatta caacttactt gctgcttctt catcgatgat cacaacaaca ttgtcgtgat
    17341 tttgcaatac acttgctggc aaatgattcg ttacaggtcc ttcaatcatc cctttgattg
    17401 catcagcttt gctttcgcca aatgccatca agacgatttc tttacttttc ataattgaac
    17461 cgattcccat tgaaacagct tttgttggta catcttctgc tttatcgaag aatcgtttgt
    17521 ttgcattgat tgttgattct gttaagtcaa ctactgaagt cgttccgtca aatgaagcac
    17581 caggttcatt gaaaccgata tgtccatttt gcccgattcc taagatttgg atatcgattg
    17641 gatgtgcatc gatgatttga tcatactttt tgcatgctgc ttccagatct tctgcttttc
    17701 catctggtac aaacgtttct ttgaatggtt ttttatcaaa taattggaca tccatgaaat
    17761 gacggtagct ttgttcatct gaaccgccta gtccaacgta ctcatctaag tttacagaag
    17821 tcatttcact gaaatccaaa tcgcttgaag tcatttcttt atataaagta actggtgtgc
    17881 ttcctgttgc caatcctaat acttttgcac ctttgttcat accttctttg atgatttcaa
    17941 atgctttttt tccgccttct tcagcgtttt ttacacgaat gatttccata gtgattgctc
    18001 ctttatattt tggtatagcc caattatagc attctttttt tctttttaca ataattagta
    18061 tagaccaaat tgcatttttt tcattttctt gacaataaat aggaatccgt gttaggtttc
    18121 ataagatggt tcaatcaaac aatccatgtt gcatgaaaac caagcaagtg attcactgtg
    18181 gacgctttat gtctgcagtt tttttatgaa aaaggagtta ttatgagtat actgacatta
    18241 aacaacatta cccagcaatt tggcgacaaa atcctctatg aaggtgtcga tctgcaatta
    18301 aatgctggcg aacatcttgg gctgattgga caaaacggag ccggtaaaag tacattgatt
    18361 aaaatcatca ctggtgagat cttaccagat gacggacaga tccattggca aaaaaatatt
    18421 cacaaaggtt acctcgatca atatgtggaa gtcgatgaaa cattaacaat cgaagaattt
    18481 ctaaaaactg cgtacgcaaa agaatacgaa aaggaagaaa aaattgcttt tctttaccaa
    18541 acatatagcg agaccttcga tgattccttg ctggaaaaag ctgggaaact ccaaacggaa
    18601 ttagaccaag gagtgtttta tcaaatcgac accttagttg ctgagatgag cagcgggttg
    18661 gggatcgatg tcttaggttt agattcactg ttgaaaaatc taagtggtgg acaacgttca
    18721 aaggtgattt tagcaaaact acttttagaa aacccagatg ttctgatctt agatgaacca
    18781 accaatcact tagatgatca gcatatccaa tggctgacgc agtttcttca ggattttgac
    18841 ggtgcgtata tcgtgatttc ccatgaccaa gaatttcttg atcagatcac cacccatatc
    18901 gcagacatcg agtttggaaa gattactaag tatactggcc atctaaaaca agcattgaaa
    18961 caaaaggaac aaaatcgcga aagctatttg cgccaatatc atgctcaaca aaaacaaatc
    19021 gaaaagaccg aagcttatat tcgaaaatac aaagccggtt cccgatcaac gatggcaaaa
    19081 agccgacaaa aacaattaga taaaatcgaa cggctgacgc ctccagcttc agcaagtaaa
    19141 gctgtctttg attttccatt ctcgcctatt gtcacgacat tagcagtgga aacaactgat
    19201 ttagtcatcg gatatgatcg accgctactt gcacccatca atttgaccat ccgctttggt
    19261 gaaaatattg ccattcgagg cttcaacggg attggaaaat ctactttatt aaaaacactg
    19321 atcggtgaga ttgaaaaaat aagtggcgat ttccacttcc cagataacac aaaaatcaat
    19381 tacttttcac aagatctgta ctgggaaaat ccattagaaa caccactaca atatttaagc
    19441 agtcaatttc caaaagcgac tatcaaagaa ttacgtcgcc aactagctaa agcagggtta
    19501 gttaatcaat tagcaagtga acctctcact acattaagtg gtggggaaca aactaaggtg
    19561 aaacttgcac agttgacaat gaaccaaggt aatttactga ttctcgatga accgacgaac
    19621 catatcgatc aagaaacgaa agaaagctta caagacggga tacagaaatt tccaggaaca
    19681 gtaatcatcg tctctcatga acaagaattt tatcaagatc ttgtgcaacg agtcattgag
    19741 attgaaccaa aataaacgtt tgattgaata gttgaaaaaa aggagctgtg acaaaagttt
    19801 tgtcacaact ccttcttctg aataaatggt ggccaattag ctgaccttcg gtattagctc
    19861 aaaaaacggc aaaatatgag gagctatttt tatttggcta ttcttattac ttgatgcaga
    19921 acagcttttc acaacctctt ttcgtctatt ttttattttc gagatttctc tttcctttaa
    19981 aaacaactgt ttgaaagaag aacgcacatc cttgaaaaac aagcccgcta accgttacac
    20041 ctaaccagcc atactgctgc cacattgtcg tccctagcaa tgaaccagcc gcacctccaa
    20101 taaagtacat aaacataaat acagtattat ttcggttgct tgctgcttca cctagccctt
    20161 gtactcgtgt ttgattggcg acttgaccaa attgattccc gacgtctaat aaaataatag
    20221 ctaaaagcaa aatggccaaa tggcttccgc caaccaataa agcaataaag cttattgttt
    20281 gcataacaag tcccatatac acgatttttc tttcagaata agaatctgcc agtcgtccca
    20341 cgataggtgc agcaactgct ccagacaaac caaaaatcgc taatatccct gcttcgaatg
    20401 ttccccaatt gtaggcggga ctgctgatat aaaaaatcaa cgtagaccaa aagatcgaaa
    20461 atgttccaaa catgaaaaat ccactgactg ccgcttctct taatagacgt tgagaagcaa
    20521 ctaaatgcgg caaactttta agtgaatcaa ggtatgaaac agctgtacta ctttgctgga
    20581 cagaacgcgg cattttccag taaagcagaa gtgtaagaat tccgactgca cataaagcaa
    20641 ataaatagat catccgccaa gaaaaaagac tggcaacaat accagatacc gtccgagaaa
    20701 gcaaaatccc cacgagtaaa ccgctaagca atgtacccat gaccttccct cttgcttttg
    20761 gtcccgctag taccgcaccg taaggaatga tgatttgcgg cacgatcgaa agtaatccta
    20821 ttaaaaaaga agcgcatgca aataacgtaa acgaaggcgc aaaaaaagcg gccaacagag
    20881 agacggcaga taacgctgcc attcgaatga tcaattttgg acgatccacc acgtccccta
    20941 aaggaactaa aaacagtaac cctaacgcat agcctaattg ggtcaacatc gtcaatatcc
    21001 cgactgcact tgtgctaaca gataaactag ctgctacttt tgttccaatc ggctggatgt
    21061 aatacatatt ggcaacaaca acgccacaag tgattgataa tagtaaaata agcgaagttg
    21121 ttaaaggttt tttttcatta ctcttcattg atttccccca tttcctatct tagtttacaa
    21181 cttatggaaa agattggaaa tagaaaaata caactattcg ccacacttat tatttgtgct
    21241 tttgttttcc ttgttttttc ttgcgatctt tataaatgaa gacaactaac cctactagca
    21301 cagtaacact tgcaaccaac gtgactgcag ttttgttata tccgtgaaac agagcttctc
    21361 caccacacgc atacaacaca gacgaaggga tgactccaat cacgatcgac aatatgattt
    21421 gctgtagagg caatttcatc gcatctgcag caaaattgac catagaagat ggaataaccg
    21481 gtatcatata gcctaacatt acacctattt ttggatgttt catttgtcgg attgcttgca
    21541 cccaatgatt cgtttcggtt ttcttatcta aaaattttaa gcgatgcata aggaaaattg
    21601 cagtgatgtt accaagagca ttacccaaaa tgttgatgat cgcacctgtc agtggtccaa
    21661 agctcaaacc agcaatgact ccaactactg aagtaggaat acctggaaca gcacacatca
    21721 ccgctgtcaa tacgatcaag agtatcgatg caaaagtccc atgcgaacga atatgtgcga
    21781 gtagctctgc cttactcgct ctcggattta aaaataattg gatgtctgtc tgatattcta
    21841 aatacaaccg atagcccact accagtatca gaatgattcc caacgagatc aatatcaatc
    21901 ggattatttt ctttttcata tttcctcctg aaaaaaatct atattcatta aataatagtt
    21961 taacataaaa acaaaaaagc accgaaatcc aacataggat ctcagtgctt ttaaatagta
    22021 aaaattattt aacagttaca gatgcgccaa cttcttcaag tttagctttc aattcttcag
    22081 cttcttcttt agatacgcct tctttaactg gtgcaggagc gccatcaact acagctttag
    22141 cttctttcaa gcctaaacca gttgcttcac gaactgcttt gataacttta actttttggt
    22201 ctccagctgc agttaattct acagtgaatt ctgtttgttc ttcagcagca gcagcgccac
    22261 cagcagcagc tacagctaca ggagcagcag cagatacgcc aaattcttct tcaatagctt
    22321 taactaggtc gttaagttct aggatagttg cttcttttaa ctcagcaacg atgttttcaa
    22381 tgttcaatgc cattataatt tcctccgttt aattgttctt ttttatttaa tgctttatgc
    22441 ggcaattaag ctgcatctcc atcttctttg tctgccactg ctttgacagc gtaagccacg
    22501 ttgcggactg gcgcttgtag tacagatagc aacatagata gaagtccttc gcgatttggc
    22561 aattttgcca atgctgtgat ttcttccaat gaagagactt taccttcgat gataccgcct
    22621 ttgatttcta atgcttttgc ttctttagca aattcgtcca tgattttcgc tggtgcgact
    22681 acatcttcgt tactgaatgc aacagctgta ggtccagtga atacttcgtc catgccttca
    22741 aggccagctt ttttagctgc acgtgaaaga atagaatttt tgataacttt catttctacg
    22801 ttagcatcac gaagttgttt acgtaaacgt gttacttcat caactgttaa tccgcggtag
    22861 tcaacgacta ctactgatgc agctgctgta aatttttcgc ttacttcatc aacgattgct
    22921 gctttttttg cgataattgc ttcactcatt tttgagtttc acctcctgaa ttttgatgga
    22981 agagtttttt gcactgaaaa ggcaagtcac ttttatagtt actttgctca accagtatta
    23041 ttcatcacct attcacttcg ttcatagtga ttctactcgc tttgctcgaa aggaaactcc
    23101 tttcgctttg ctcaaccagt attgttcatc atctcttcac ttcgttcata gtgattcaca
    23161 ataaaaaact ctatgccacc gcagacatag agggactatt gaatatattc aataaacgtc
    23221 ctcggtagga aattaaggct tgatgccacc tactgtcttc ggtacaagtt aattattaac
    23281 ctcaactagc ttagcatgct aaactagttg agtcaagaat tatttttcag ttggatcaat
    23341 caacattcta atatttaatt agaaagaagc ttggtcaaca tggataccag gtccaaatgt
    23401 tgttgtgatc gttacatttt tcatgtattg acctttagct gctgatggtt tagcttttag
    23461 caatacatca tggattgctg caaagttttc aactaatttt tcatcgtcaa atgaaacttt
    23521 tccgattggt acatggatat ttccagcttt gtccacacgg taagttactt taccagcttt
    23581 cacttcgttt actgcttttg ttacgtccat tgtaactgta ccagttttag ggtttggcat
    23641 taagcctttt ggtcctaata ctcgtcctag acgacctaca gtagccatca tatcaggtgt
    23701 tgcaacaact acatcaaagt caaaccatcc gttttggatt ttttgaacca tgtcgtcgtc
    23761 acctacgaaa tctgcaccag ctgcttcagc ttcttttgct ttgtctcctt tagcaaaaac
    23821 taaaacagtt tgtgttttac cagttccgtt tggtaataca acagcaccac ggatttgttg
    23881 gtctgctttt ttagggtcta cgttcaaacg gtatgcaact tcaactgttg catcaaattt
    23941 tgcaatgttt gtttcttttg ctaaagctac tgcttcggca acaggataaa ctttacttga
    24001 atcaactttt tttaatgcat cttgcatttt tttgctcttt ttagccattt tggttcctcc
    24061 ttgattgtgg ttataacggt tgtacctccc acgtgttcta tatctttcaa tatagagcca
    24121 actgagaagc tgctgtctta gggtgtatag attattctac agtgatcccc atgcttcgtg
    24181 cagttccttc aaccatgcgc attgctgctt caacgttagc tgcgtttagg tcttccattt
    24241 ttaattcagc gatttctctt acttgatcgc tagaaacttt cgcaacttta tttttgtttg
    24301 gttcgcctga acctttttcg attttagctg cttttttcaa tagaacagca gctggcggtg
    24361 tttttgtaac gaatgtgaat gaacggtctt cgtatacaga aataacaacc ggaatgatca
    24421 aacctgcttg atcagctgta cgagcgttga attcttttgt gaatcccatg atattgatac
    24481 ccgcttgacc tagtgcagga cctactggtg gagctggagt tgctttacct gcaggaattt
    24541 gcaatttaac gattttttct actttctttg ccacgagaca tacctcctta agtccgtgat
    24601 gtggttaatt ggagatgcat atttctcctc ccacgtttta tctgatctga aaaaatccag
    24661 acaaataatg tgcaaaacaa tgcacactgg tacagtatag caaatttatt tgttttttca
    24721 agattttttt ctgctatttt tacattttgc ttgaataaac gctaaattta ttctaaaaaa
    24781 acaacaattt ttcgttctca tttcttcttt tatgttacac ttgcgctagc atggttcaac
    24841 agatgaaagc aaacaataac acggtttttt agacccttgt acaaagattc ctacataaca
    24901 aaatgaggtg gagaaaatga aagatttaag gtatagaagt gtctttgata ttattggacc
    24961 tgtgatgatt ggtccaagta gctctcacac tgccggagct gctagaatcg ggaaaatcat
    25021 acgtagtata tttggcgaac agcctgattc tgtagatatt tatctatatg aatcatttgc
    25081 aaaaacctat cgggggcacg gaacagatat tgcattagtt ggcggcctgt tagggatgga
    25141 acccgatgat gaacgtttag ctgattcttt gaaattggca tatgaagccg gtatggaagt
    25201 cttgtttgtt cctaagagtg aaaaagccga acatccaaac tctgtaaaga tgcttctaaa
    25261 aaaaggaaca cgaaagcttt cagtcactgg catttcgatc ggcggtggca atatccagat
    25321 ttctgaattg aatggattca aaatttcttt aagcatgggt actcctacct tgatcgttgt
    25381 ccaccaagat gttccaggta tgattgctaa agtgactagt atactttctg agaccaatac
    25441 gaatatcggc actatgacag tgacacggga atcaaaaggt gaaaatgcca ttatgataat
    25501 tgaagtagat gatcctcaag tagaagaaac ggtaaaaaga ctcaaacagc tgccaaatat
    25561 cgacagcgta aattatttcg attaaggagc taaaaaaatg tttttatcga ttgaagaatt
    25621 agtagatcaa gcaaaagctt atccaagtgt agcagaattg atgattgcag ttgaaatgga
    25681 aatgagtggc cgttcccgta gtcacatcat cgagatcatg gaaaaaaacc tctcagtaat
    25741 ggaacaatca atcaacgagg gaaaagaagg tgttacctcc gtcacaggaa ttaccggtgg
    25801 tgatgcaaaa aaattagatg attatttaaa tgaggcagac tttttaagtg gagaaacaat
    25861 cttaaaagct gtacgcaatg ctgtagctgt taatgaagtc aatgcaaaaa tggggttgat
    25921 ttgtgctaca ccgactgccg gaagcgcggg tgttgttcct ggtgtgctga tggctgtccg
    25981 agatcggctt gatttgacac atgaacagca attggaattc ttatttaccg ctggcgcttt
    26041 cgggttagtc atcgcaaaca acgcttctat cagtggtgca gaagggggat gtcaagcaga
    26101 agttggttct gcaagtgcaa tggcagcagc agctttggtt tgtgcaaaaa atgggactcc
    26161 tcatcaagca gcacaagcag tagccattac attgaaaaat atgatgggac tgatttgcga
    26221 tcccgttgca ggcttagttg aagtcccgtg tgtcaaacgt aatgctttag gctcttctca
    26281 agcttttatc tctgctgata tggcattagc gggtatcgaa agtgtcattc cgccggatga
    26341 agtcgttgct gcaatgtacc aagtcggacg tcaaatgccg tcgattttca aagaaacagc
    26401 agaaggtggc ttagctgtta cgccaactgc taaacgactt gcggaagaga ttttaaataa
    26461 gagggcgtga taaagctcct taatttcgag tattaagaga ttagttatct tcataacaaa
    26521 aaacgaagag aggactaatt gcccttctct tcgtttttta ttaatccatc aatttcgtat
    26581 tttttacttc atcgatcgtt tttgttcctg caagttgcat cgtgatcgac aattctttgt
    26641 tcaggtgttc aaaaacagat gttactcctt ctgccccacc tagattcaac ccataaatca
    26701 ctggtcggcc aatcgcaact aagtcagcac cacttgccag agctttgaag acatgctctc
    26761 ctcgacggac accagagtcg aaaatcactg gtacacgttt gttgactact tcggcaattt
    26821 ttggcaatac ttcaaaggac gcaggacctc catctagttg acgtccacca tgattggata
    26881 cccagatacc atctgcccct gcagagatag cgattactgc atcttctggc gattgtattc
    26941 cttttacgat aactggaaga tgggtgattt ctttgattgt tttgatgtca tctggtgtca
    27001 agccttgttt tgcagctgca taaatttctg cgattccttt tccttcgcca ttaccacttg
    27061 cactttgttc gctatacgca gctaagttag gcattggtaa tggaaattgg aattgattga
    27121 tgacgtcttc ttcacggtaa ccaccgagcg ttgaatctgc cgttaaaatg atagctttag
    27181 cccctgcttc tactgctttg ttcaaaatga actcattgaa tctgtcgtct ttactcatat
    27241 ataattggaa aaactgcggt gcgcctggcg ctgcatcggc tgcgtctttg attgtcgtat
    27301 ttgcataagt gctgatggaa aagattgatc cagcagcagc tactccttta gctgtatcag
    27361 cttccccttt ttcatgtgcc aatccttggg ccgctgatgg cgcttggata atcggtgtat
    27421 ctaattcaat tccgaaaaca gatgtatgca aatcggctga gtcgattccg cgtaatatcc
    27481 gtggcatgat tttctttgta ttaaaagaag tcgtattttc tttcatcgtc cactcatctt
    27541 cagaaccacc gcgaatatag ccaaaagctc ctgcttccat gcgctctttt acacgacctt
    27601 ctagttaggc aatgtttaca atatcgattg gatgttcttc attacttgta tgataagtca
    27661 tagtagatcc ttctttcatc ttcttgatta catataaatc atactaaact tacaaaaagt
    27721 aagcaaacat ttactctcag atacttttat cgctattctt tccaaataaa tcgacttaca
    27781 agctgatcta cttcttggtt ttcatgaagc atactatgag aggcattttc acctgtcact
    27841 actttttcct ctatttcatt tccgcgttgc ttcaacaacg catagaccgc taatgcactg
    27901 tttaacggta ctgttccatc actcaagtct gtttcatcta gttgaccagc aatcaagaaa
    27961 aatcgagtgg ttataggaat attaccgatc atttgctggt aatcttgata gcggttagaa
    28021 acgacagcag gcccctttgc taactcatct gtcagagact gcgcactatc atcgacaaaa
    28081 tcattaaaag gcgcgccaat tgctgttaat gtctgaactt gtggcaatga cgtatcctgt
    28141 ccatacgttc ctaaatagcg tagtgcacta actcctccca tggaatgccc cactatgttg
    28201 actttatcta tgccgtagtt ttctttcaga tacaaaagac aagttttgat ccattctgct
    28261 tgattccact cgttattttt attatcttca aacaatactt ggaccatcgg attgtctgct
    28321 tttccagaca aatctcctgt tgcttgtatt tgtccatctg ttcctactgt taaaaccaat
    28381 tcttttactg cttgctgatt tttttccaaa cgccgcaaca tccctttaaa ggaattttct
    28441 gtcccgctgt agccgtggaa aaaaagtgtt ggaatagtgg ttttttctat cactttactg
    28501 gattctgccg ttttttcttt atcagaaact tcagtagtat ttatcttcgt cgtctggcaa
    28561 ccacttaacg caaacaaact gataaggact aaagctgctg cagttgcacg tttgcataaa
    28621 tttttgattg gttctgtcat acacctcttc ctttccatat agaaaaaaga aagacttgaa
    28681 aggccaatca tatgatcgac atttcaagtc tctttattaa tcgattttat ctacttgatc
    28741 gaaatcaagt tctgtactgg tttcacgtcc aaacatatcg atgttgactt tcaatttttc
    28801 tttttctccg tcaacttctg ttacttgtcc ttctaatcca gcaaatgcgc cttcgatgat
    28861 tttcacgact tcgccaactt ctacttctag atcattttgt cgcaggctca taccgataga
    28921 acgcaagata tgattgactt cttctggaag taatggtgct ggtttgcttc cggctccgtg
    28981 tgaaccaacg aatcctgtca caccaggtgt gttgcggaca acataccatg aagcgtctgt
    29041 catgatcatt tcaacaagta cgtatcctgg gaatgtttta tgaacgattt ctttttcttt
    29101 cccatttttg acttcagttt ctttttcttc aggaacaacg acacggaaaa taaagtcggc
    29161 cattttcata ctttgcgcac gagattcgat atttgctttt actttgtttt catatcctga
    29221 atatgtatgc aagacatacc agtttttttc aaaagattcc attgaaggct cctcatttca
    29281 ttgtaaaata aaaaaacctc agtacacccg aagtttttct ttcgcttcat tatagcacta
    29341 aaagtacgtt gtggctagcg agttatttta aaatccagcc aaataagctt tggatggctg
    29401 tatccattac gtaaaacatc aatccgaaaa taatagaagt ttcgatgact actaacgtat
    29461 ctttacggag ttgttttctg cttggccaag ttacttgttt gatttcatct tttacgcttt
    29521 ttagaaattt catgagacaa cctcccttgc tactgtttat ttcgtttctt tatgtaatgt
    29581 atattgatta caatatttac aaaacttatt gatttccaaa cgttcgccgc tttttccttc
    29641 actgactgat tttgtgtagt tgcgagaccc acaaacggaa caagcgagtg ctgctttttt
    29701 ctttgccatt tacttctgcc cccttgctga gaatcagact tatacttcat aacagtatca
    29761 cgtaactttt tacttgtcaa tttgtgaaat aaactatcta ttgttttgga taaaattatg
    29821 atatgattaa tttgaaatta agctaaaatg agacaattgg aggtatagcg atgctattaa
    29881 aaacacttgt atataagaaa aaagatctga caaccgttac agagactagt acattagaag
    29941 aagcattaaa aattttagaa acttccggat accgttgtgt gccgatctta gattcttcag
    30001 gtaatatttt ccgtggaaac atttacaaaa tgcatatcta tcgtcataaa gcaaatggcg
    30061 gtgatatgac gcttcctgtc actcatcttt tgaaaaacgc tacaaagttc atttacctgg
    30121 atacttcttt cttcaaagta ttctttacga tcaaagagct tccttatatt gctgtccttg
    30181 acagtgaaaa tcatttttat ggtattttga cacatagtac gttgcttaat atgctctctc
    30241 aatcttggag cgtagaacaa ggaagctatg tactgacaat tgcttccaca ggaaagcaag
    30301 gagatttggc tgctattgca aaaatcattt caaaacacag cagcattgcc agctgtatta
    30361 ctcttgatat cgaaaaagac gaatacattc gacgtacatt gatcacgcta ccggctaaca
    30421 ctgaagcgaa agtatgtgaa acgatcgtca accacttaga gaaaaagaat tataaagtgg
    30481 tagaaattga aaatttgcaa aaccaagaag cataatcaaa aagcgaaaaa agaaaacagc
    30541 taatctgctt tcttttttcg ctttttataa ttagacacgc ttttcaaaat caagcgcgcg
    30601 atctaaataa tacatcaatg gtgctgaaac agccatgacg ataaactcac ttaatgcaag
    30661 cgtgccatag gttgtccaaa atgcttggcc acctgttgga actaacatat aagcaatcag
    30721 tcccatgctg atggtaaaga agatcgtgtt caatactaaa cgcatcttta catttttcac
    30781 tttgttttgc aataaagctg tcaggcctaa agaaagcaat gtttggccgc caccaaaaac
    30841 cacatccatg ataccaaaac caaagaaggc attatagacg atgaccccgc ctaaaactcc
    30901 ccaaagcaat ttacggttaa agacaactaa atgattcaaa ctttctgaaa tacgaaactg
    30961 gatcggccct gaggatacag gcgcaactaa aatagttaac gctagataaa gagccatgat
    31021 gatgccattt acaacgatta cggataaccg ttgtctgctt ttcgtttttt gcattttctc
    31081 tcctcttttc tcctagtttt tttaacgcgg gatggttgat gaaccgcggt tatgtgaaac
    31141 aaacaataat tataacagac ttttcattta tttcaatccg tcttatctgc ttttaaaaaa
    31201 gcaaaagctt aaacaatgta aacgtttgct aaaaatcgtg ggatatggta taaagtaagt
    31261 gtaaggggtc ataagaattc ccagtaaagc agacgaagta ggatctgcaa gaacagaatt
    31321 gttgtgaaac ttaaataaat aaaaaaggat gtgggaattt gagcaacctc ttactagaga
    31381 acggtctagc aagcagctga agatctaagt ggacatgccg aaccttggaa cttctcctta
    31441 cgtaagtaat ggatcatgcg cttgctaggc ttttttgtgt ttttcggtct tcggaccttt
    31501 ttcattttcc tagacttctg ctataataac caagaattaa ttttgtaaca gatttttttg
    31561 aaaaaacaag tttctttcat tcaaaaaata gaaaaagctt gctaattatt ggttaaccag
    31621 caaacaggag tattaaaata tcttgtttta ttgaatagca ggaggaaatc atgaaattcc
    31681 attatcatat attgatcaat ccatcggcag gcagcggaaa tggctataaa gtagcagaaa
    31741 gaattttacc ggtattaaaa aataagcata ttgactatac acttcattat agtgaataca
    31801 aaggacacga tgctgagatt gctgaaactt tggcaaaaga aacattgatt ccttggatag
    31861 aagaagaaca gaaaacaatc gatactttcc cccttttgat catcgtagga ggtgacggca
    31921 ccttacatca ggtacttgat actttctatc agatggaagt agaattccca gttgcttata
    31981 ttccagcagg ttctggagat gattttgctc gaggggcagg ccttccaaaa aatcccaaaa
    32041 aaggacttca gctgatttta gcagctcaat caccagaaaa agtccacata atggcttatg
    32101 aagaaaaaat aagtgaaaaa aaaggaatag ctgttaataa tttcggtata ggattagatg
    32161 cagctatcgt ccatgcgaca aatcattctt ccacaaaaaa gcgattgaac aaatacaact
    32221 taggatcatt ttcgtattta ttctctattc tccgtgcttt attcactcaa aaaggatttc
    32281 caattctggt agatgcagga ggaaaacaat taagtttttc aaacgcattt ttatgtacag
    32341 caacgaagca tccttttttt ggcggaggaa tcgcgattgt tccaactgct gatgcgtcta
    32401 aaccagtcat tgattttgtc gtggtcgaac gaatcaattt ctttaaaatc ttgtggctca
    32461 ttcttttact tatacagaaa aaacaattga aatcgaaata tttccatcac tacacaacaa
    32521 acagattacg tatcgtttca acaacaccgc aacatggtca agaagatgga gaagaaatgg
    32581 aaaaacgacc ttttgatatc acaataacca caactaaaca gttgttttgg tgttaataaa
    32641 agaggttttg tcagaagcta catctggaac accgtttatt cggaaaaaag gactgtgaca
    32701 cgacttttgt cacagtcctt tttttactat gcagttaatt ctttgcccaa ttgggttgcc
    32761 atcgatagtt tctttcttta cccaaaataa attggatcat tgaaaacacc gcagcaaaag
    32821 caactaagac acaaccattg aaccaaaaag attctgcaaa tgtttgattg taactgagtg
    32881 tttcactgat caagttatta ttttgaacaa accaatatgt tggtgtgaat gccgctattt
    32941 tgctaaccat ttctggcaag tattctgctg gtacaaacac cccaccgata aagcaactgc
    33001 ctgtgataaa aatatctttg atcccaccgc tggtttctga gttttttacc aatgaagtga
    33061 tacaagccga aaagctaatg atcggcaata aaaacaagaa agtatttaaa atatgatacc
    33121 ataccgttat atcccacgag gtgtggatca ctaacagaac cattagaagt aagaagccac
    33181 tgaacaacag cagcgagtaa ctaatcaaac taaaggttac tttgcgattc aagtttcgtt
    33241 tggtaatggg cgcactctgg tttcgtttac tgatctcttt gcgattaaat gctagattga
    33301 tgacgctgaa accgctaaaa atggatagaa acaacccata agacaagaaa ttaaaggttg
    33361 tgcccgtcaa cagctttttc atccgctgac tatatcccgc acttagctca acttctccag
    33421 taaccgagag tgccatttgt gtctgttcca tagctgcatc gattgtttta tcttcttctt
    33481 gaaacagaat cagtgtttgc aaaaaggtgt tcacttgctg tgtaacaact gttttagaaa
    33541 aggcatctgg gcgactctgt atcttgatcg tcggcatctt tcctacttcg acttgttcgg
    33601 taaagtctgc aggcagatgt aagataatgt ttacacgatt aaaataaaga gcatcatcga
    33661 tttctttttg tgaggttcca tccaatgtaa cgactgtttc cttgtcattc aaatatttac
    33721 gaaggccatc gactacaggc gactcttctt cactaatcaa ggcgattttc gcatcttgca
    33781 gagaggtatt ttcaacatca atattttgcg caaaaaagaa tgtggcaaca acagtgataa
    33841 ttacacctat cagtaaattc gatttatttg ctttaagcaa tgtccaaatc gttttataaa
    33901 gcgtcataac gcacccctct ttccatccaa gcatttccta acgcaaaaag gaaaataaat
    33961 ccgatcaacc atagaagatt ggtataaaaa ctagccaaat cagtgtagaa ataaagttta
    34021 tacaaacttt cgctgatcaa gttgacgaga tttaattgac caacaatcgg aatatgaaga
    34081 tcaatccaat acttcacttg ttccgacccc atcataccag caaaaaagct catcgcaatc
    34141 gtcactgaaa tcaagatgct atccttttgc accagattca tttttggtat caaattacca
    34201 accaatgtac ctaacaaaat cgcacacaac gaccccagag cggtaacaat catcaaccat
    34261 tgccaacgtt caccaaagtc tacttgatag ataaaacgaa agacagctaa cataatataa
    34321 atctctgtca aaacaatcgt aaagcttgct gccaaattag cgattgaaac taacgattgc
    34381 tttctaggaa tcaaggacaa gcgaatacca tttgacgatt gatttgcttg ctgatcctgt
    34441 acattgcgga ccccccacat cgtcccgtag agaatcgcca ttccaatcag agtgaagaaa
    34501 taaaaacttt tgaaactcat attacgacct gcatgaactt cttgaaaaga tacttcttgt
    34561 tcaaaaacag tcatgatggg cgcgagatca acgttctcca cttctgccac tttactcgaa
    34621 attataccaa tacgctgtaa aaattgatcc atgacgtttt tcatcaacgt ctgactcatt
    34681 cctgcttgac ccacatggag cgaaacagat gaaccatcaa tgagataaaa accagccaaa
    34741 tcatctgcat ttagcttttc attcgcttct acttccgata gttcctgtac ttcaaataat
    34801 ggtttatcat tgacttcgac ttgttctaag actgtggtaa aattttccga aagcgctgtt
    34861 tgatccgctt tgattacaat tccagtatta atcgtttcga gattgccacc ttgatcaatc
    34921 tcgccaaagg ccgccataaa catcaatcct aaaagaattg gaaaggccaa tgtccaaaac
    34981 aacatggatt tatttcgcag cagtattttt gtgcgatatt tataaaaatg ccaaaacatt
    35041 atttcccctc ctctagtctc tcaaatcctt gccagtaatc tctaaaaaga catcattcaa
    35101 ggacgcttct ttggttgcta tttttccgta actgatcttt tcttcttgta agattgtaag
    35161 aatcggcaac aatactgtat ttagttcagt agttgttact tggagctgac cttttttata
    35221 agcgattcca tcagaagcta ttgttcgctg acaacacgtt ttctgggagt aatgggatgt
    35281 cgatcgtcaa acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag
    35341 caatcacctg tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca
    35401 tgtaatgtgt tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta
    35461 aaattttatt ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc
    35521 gcggcttgtg ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt
    35581 ctcctggacg aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca
    35641 aggctttcct tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt
    35701 gcacacttag ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga
    35761 cagcataatt ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat
    35821 ttaacaaaga aagcatacag ttgatggcag tcgatttccc actgccgttt ggtcctaaca
    35881 gacctaagac ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc
    35941 catagcgttt gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt
    36001 cttgttgatc tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca
    36061 cacatgacat ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa
    36121 actagctttc tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg
    36181 caagttatct atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca
    36241 aagccaaatt ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt
    36301 gtcctgtttc tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac
    36361 ttgtttttgt taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc
    36421 gcctttgtcg taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg
    36481 accgatcttg agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag
    36541 ttatcggaac aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt
    36601 gtggaagaac ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca
    36661 agtgcgattt tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa
    36721 ccattagaaa atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc
    36781 catgacctcc atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat
    36841 tttccttctt ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa
    36901 aactgcttct taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc
    36961 aaaaccgttg ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat
    37021 gatggcacca gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa
    37081 cgtgttcaag aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta
    37141 acgatcattt taccaaggga gggacaccaa tgataacagt agtcattatt gagatgatcc
    37201 atttgtgacg aaatcgttac aaaccatttt agaaagcacg aaagagatcc gtgtgttagg
    37261 cattggtcat tgtgccaagg atgcgctagt cttatacgaa acccatcgtc ccgatgtttt
    37321 gctgacggac atccgcatgc ctaaacaaac gggcatcgat gctgccaaag aaattttggc
    37381 taaatttccc aaagccatcc ttttgctttt gacgacattc aaagatgaag aatacatccg
    37441 cgaagcattt tccatcggtg tcaaagggta tttaatcaaa cagaacttgc aagcgattat
    37501 tccttctgtc aaatccgctt ataatggaca ggccgtattt ggtaatgaaa tcatcgagac
    37561 tttcactcaa atgatgaaga ataaaccgac gattgatagt catgcttatg cttcttttag
    37621 tgaacgtgag cttgcaatca tcaaagaagt agctgccggg aaaaacaata aagaaattgc
    37681 agatgccttg tacttgagtg acggcactgt tcgcaattac atcagccaat tactagaaaa
    37741 gctagattta cgggatcgca cgcaattggc tatctatttt tatcagcatt tgcaataagt
    37801 cttatctaac acaaaaaaac ctgaatgatg ctccttcgca tcattcaggt ttttttcaca
    37861 tcttctattc aattgtcccg caaaccattt cctcacgatt gaaagaatca aggattcgca
    37921 gggctgccag gattcgttgg attgttcggc gtgttaggcg gaaaaatagg cgccttgtct
    37981 aacgtttttt ctagttttga ttcttcacga tcgtctcgtt ctacatcagg actttcattc
    38041 aatttgaatt ctttttccat ctcatctaac tttttaaagt cattgccaga ttcttttgct
    38101 aatttcgcta atttatcttc ggtttcttta gccataaagt aacgcccccc ttttgatttc
    38161 agcttagcat atcacactga aaaaacaaaa gaaaacgcta accaacgatc atttttagct
    38221 atttctcatc tttaaaatca agcatttttt gccggtataa aatcgtaggg agaactgttt
    38281 tccataccaa tcatcggatc tactaactgg ttatcgatca cttctgctgt aagaacgaaa
    38341 tctttagaaa cagcaggttc tacctcttct ttcttttctt ctccaacaaa tgaagaaaac
    38401 agatctggtt ccactgtttc cattacttgg tttttcgttt cattcgtccc actgtcatct
    38461 tctacaggta gcttttcttt tacaacccgt ggctcttttg tatagttccc atcttcaaat
    38521 ggatcatttc cgaccaaaga atcttcataa gctgctagtt tggtccggag agtgatattc
    38581 attttatcat tatccatgat ccttgatttc aacgtagtca tccggcttgc tgattcattc
    38641 gctacacaag tgacataggc ttgctcttcc cctaacaaaa tcaacagttc cttactgcga
    38701 gtcactgccg tgtagagcaa attacgctga agcatccgct gatattgatg gaccatgggc
    38761 aaaataacca tcttgaattc actgccttgt gatttatgga ttgagcaaca ataagaaaga
    38821 gtgattttat tccattcgtt tcttttataa gtgacctcat ttgcatcaaa ctggataact
    38881 aattcatcca ctttatcttc agaatctttt gccaagatga tacccgtgat ttctcccata
    38941 tctccattga atacattcaa ttcgggagta ttgactaatt ggaggacttt gtctcctatc
    39001 cgataaacgg tgtcgttcca atgtacttcc ttttttccag gtttccctgg attgaatatc
    39061 tcctgcatca ttttatttaa cgcatcgatc cctgcaggtc cccgatacat tggtgctaat
    39121 acttggatat cttgtgcacg gaatccctta tttttcgctt tttccacaat tttagagatc
    39181 aagggttcaa tttgataagc atcacttcgg aaataggaac gatctttttg attctttgtg
    39241 aaatcttttg gcagacgtcc ttctttgatc tcatgggcaa gaggaataat actagaacca
    39301 tcaccttgcc gataaatctg attgagttcc attttgggaa tttgttcgat ttccaaaaga
    39361 tcatggagta cttgaccagg accgacagag ggaagctgat ctttgtctcc tactaagata
    39421 acttgcatat tcgtcggaat cgctttgaat aatgtgttag ccagccatgt atcgaccata
    39481 gacatctcat caacaatcaa aagtccgcct tctaattctt tcgtactgac tgaaggtgtt
    39541 ttttcccgtc cattcagccc cagaagacga tggatcgtac tggcaggcag acctgtcgtt
    39601 tcgttcattc gtttagctgc tcggccagtt ggtgctgcaa gtagaatagg aaaaatttct
    39661 tgggtatatt ttgaaggatc caattccagt ccgttcaact ctgcaaaaag attgacgatt
    39721 ccgttgatca cagtagtttt ccccgtacct ggtcctcctg ttaaaataaa tagaggggaa
    39781 cggattgctt cagcgatagc ttgttcttgc gaaggtccat actgtatgcc gaatattttt
    39841 tcgatacgcc gaatattctt ctgcacttct ttttcaggat aatggatttc ttttttacga
    39901 ctgagaaggc gctggatcga attaccgatc ccccattcag aaaagtacaa actgttttcg
    39961 taaagattag tctcttcttg ttgtatcttt ccttcttcaa caagttggat aattacagta
    40021 gcaacctgat caggctggat ttcaattggg cgacttgttt ctaaaatacg gatcgtttgc
    40081 tgaagcaatg tctccgccgg tacgtatgta tctcctgttt gaactgcatg ctgaaaaatc
    40141 tgatgcaaga tagctgctcg aattcttctc ggtgaatctg ctgcaatacc gatttgttcg
    40201 gctagattat ctgcacgttt gaacccgatt ccttctatgt cttcgactag ctggtacgga
    40261 ttttcttcga tgatatccaa agactcgttc ttataggcct ggtagatact gaaagctaac
    40321 tgactaccga acccgtaacg attcagtccc atgatcactt gatccatccc gtggtttagc
    40381 cgaatcgtct cagcaagcat ttctcttttt ggcttcgtca aaccggagat ttgttttaat
    40441 accgaaggat cagcaatgat tttttcgata gcatcttctc ccagcaattc tacaattttt
    40501 tctgctgttt tcttcccgat tcctgggaat ttttcgctgg aaagatacgc tacgatccca
    40561 tttgcagttg ttggcttttc ttgctcatat ctttcgagtt taagctgcct tccgtattta
    40621 ggatgatcca ctaattctcc ataaaaacga taagtctcat tttcttgaat atcgccgaaa
    40681 cttccggtga ctacaatctc tttttcacgg taatcagctg tggtttcact gatgtgtacc
    40741 aaaagcactt tgtaaaaatt actaggattt tgaaaaaaaa tcgcggcaac tgtaccaacg
    40801 aaataaggca tttcttctgg ctgcatcgga agccgcctcc ttcttctata gtaaaggttc
    40861 tggtgctgtt tcagttccta aaaaatctgt ttggttccat actttcagtt catatggcaa
    40921 taatctgcaa ggctcttcag tctctaaaat cgtcaaacta ctattgaaca atccacccat
    40981 ttcacgcaac tcacttagct cttttcctgt catccattga atggctgcag ttaaagaagc
    41041 tccatgtcct acgaataaaa gtgggccatc tttatgttgg gcaaccgcat ctgctacaac
    41101 agtttcgata cgtgtgattg ccgattcaat cgtttctccg tgaaaaggag aaggatcata
    41161 cagatctaaa tgattacgca aattcggcag attatccgga tatttctggt acatttcatc
    41221 gatcgactga ccttctaaag tacccagccc tagttctctt agcttatctg tataaaaaat
    41281 ctctaccgga cgagcaagct ctttgttgat accttctgct gtatcacgtg ctcgctttgc
    41341 tgtactggaa taaattgctt taaaaggaac atattttacc tttttcccaa atgctttgat
    41401 ttcttcgtaa ctttgtggca aaagcggaga atcgccattg ctcccttgaa aacgtctttc
    41461 ctgattccat tgtgtcttcc catgtcgggt aaaatatagt tccatacgca gcaaactcct
    41521 gtctttcttt tattttactc tataataaac tgtctaatcc agcttgtcat tttcatagtg
    41581 atgcgtcaat tctaaattag ggaatccttg ctggcgaagt gcttcataca ctatcattgc
    41641 agctgtattc gataaattca atgagcggac atgctcatca ttcataggga tgcgtaaaca
    41701 cttttcttcg ttttcacgca taaatgtttc tggcaaaccg gttgtttctt ttccaaataa
    41761 aaagaagtgc tcttctccat cagcaaaatt ttcgtcacta tatactttat gggcaaattt
    41821 tgagatcaaa tgtagtttac gattacctaa aaatgttaaa aaagcttcta aattactgtg
    41881 gtaagtaata tttacatcat tccaataatc caaccctgcc cgttttagat gtttgtcatc
    41941 agttgaaaaa cctagtggct cgattaaatg aagcggagta ttcgtggctg cacacgtacg
    42001 ggcaatattt ccagtgtttg cagggattaa tggttcaaat aaaacgatat gattcatcag
    42061 gatcattcct ctcatattta tgttcgtttc tattttctga caatttgatt tttgcataaa
    42121 aaaaaacgta tcaactcggt gtcagtcact gcgagtcgtt acgttagtga agctcttctc
    42181 actaactttt atcagaacag gaacctgata aaaaccaatg aaaaaacaac ttgttgctaa
    42241 tataacaccc tcaacttttt tttgcaacct ctactcatta aaaactgcta gaaaaacgtt
    42301 ttctcttagt tccttttaat aattctatgt caattgtaat cttcttcaaa ggattcttat
    42361 ataagtctgc tttgattcgc tcttctacct gccattctaa aggtgacatg gccaaaagat
    42421 ctttttgatt ttcctctggt atctcaaatt cataggttac acgctgacgt tcagttaatt
    42481 tcattgcttc agcaaacttg gctactacac gttcattgga ataactctgt ttttcaggtt
    42541 gattcggata aaaagcctga cgtagttcct ttaaataatt ttcttgtggc accaccttca
    42601 acacttctcc atcttctgag agtacacgtt ggaattcttg gtaatgagaa ggcgaaaaga
    42661 tatttaaaac cgcgttgaat gtttgatcag caaacggcaa attggtcaaa tcagctacac
    42721 accaaaatgc ttcggcagat tgttctgtag ctgcgtagac tccttctttt gaaatatcaa
    42781 atccaacagc attttgaatc tttttcaatt ttgaaagttc attcaagaag ctaccttctc
    42841 cgcagccaac atctaatagc gagacttctt caggtaaatg ctgcgcaata gtctcaataa
    42901 cgggctgata catgccagat tggatcattc tacgccttgg aacgaacatt ccttgatcgt
    42961 attctgtttt aaccgcatgg gataaaaaat aaagtgttcc ttttttggaa aggtcaaaac
    43021 ggtgattatt tttgcatatc aagccattat cagacttttt tacttcttct tggcaaaccg
    43081 gacagcggaa cagctgatga ttttctgcca aaaactggcg tcctcggtca atttttttta
    43141 acatagttcc ccctcatttc tgttttatct taccgttaga tgctcacttt ggcaactgct
    43201 aaaaatatga tacaatgcct agaagaaagt gaaagataaa ggagaagatt agatgatcaa
    43261 agaattttgc gctgaaaact acacatctat tcctctagca attgctagag gagccgatcg
    43321 gatcgaattg tgtgacaact tagcggtcgg tggcacaacc ccgagtactg gtgtgatcga
    43381 agaaacgtta agttatgctg gggaaaaaga tatccccgta atgacgatta ttcgtcctag
    43441 agggggcgac ttcgtctata acgatatcga attgaaaatc atgcatactg atttgattga
    43501 agcaaagaaa ttagggacgg atggcgtagt atttggttgt cttactcctt caggctggct
    43561 agacgaagag gccttagaac tattgatcga aacagcagaa gggttgcaga tcaccttcca
    43621 tatggctttt gacagtattc ctgaagatcg gcaattcgaa gcaatcgatt ggctggtaga
    43681 acatggtgtt gaccgtatct tgactcatgg cggtcctgct gacaaaacta ttgtagaaaa
    43741 tttcccacgg cttaaagaat tgattgccta tacagatggc cgcttaatca ttttgcctgg
    43801 tggtggtgtc actgcagaga atgcagcaca tgtagctaaa gaattgaacg taagcgaagt
    43861 acacggaacc aaaatcgtgg atcttcagtt ataatcttat aaacaaaaaa acatttgcaa
    43921 caggcataat gcttattgca aatgtttttg tttttttaca taaaatagcc cataaataaa
    43981 gaagcaatat tgaaataaat cagtacactc gtcaaatcgc tcaaagtcgt gataaaggga
    44041 ccgcttgcta ctgctggatc aaatcctaga cgatccatta gcatcgggat taaactacct
    44101 gcaagattcg ccactgtgat tgcaaatagc atagccatcc ctatgacaaa ccccaagata
    44161 aaattatgtt tccaaaaacc tacgattaga aaaatcgtga tccccgtaac agctcctgtg
    44221 acaacgccgg tcaagacttc agccaaaaca agtcgaacaa atccatgatc tttctcatca
    44281 gaaatcgcaa gtcgacgaac agccacagct agcgactggg ttccagcatt accggcagtt
    44341 cctgtaatca gcgaaatgaa gactgctaaa atactcgctt cacttaccaa gtcttcatag
    44401 tgactgatta aagaagcggt cgacatacct aaaaacaaca gagtgatcag ccaaggcaaa
    44461 cgtcgagacg ctgcctttac tggattttca ttaatttctt ccacattgac cccggcaagt
    44521 cctgaataat cgctagcagc ttcatcatca ataacgtcga tgatatcatc gactgtgacg
    44581 attcctaaca aatgatcatc ataatcggta acaggaagag ctaagaagtc ataatctcta
    44641 aatgtttgtg ccacatcttc ttggtcgtcg cctacatgaa ctgaaagcac tcgttcgctc
    44701 atcagatcag aaatcattgc gtcgtcatca ttcacgatta ggtcgcgcag agaaataacc
    44761 cctactagct gaccttcttg attaacgaca tagatgtaat agattgtttc agctacatct
    44821 gcttcgttct tcagcacgta catcgcagag cgaacagttt gattggctac gatcgaaaca
    44881 aattcagttg tcatgatcgc gccagccgtt tcatcctcgt aatggagaag ttccttgatt
    44941 tcactggcat cgtctgccga catcaaactt aaatatttag caatttgctt tttatccagc
    45001 gtattaagca agtcaacagc attatctgta tacatctcag aaagcatatc agctgcatag
    45061 ctaggacgca tttcatctag gtattctttc atatgttcgt tgtcttcttc aatcacatcg
    45121 aacatatccg ctaattcttt aggcgacaga taggaataga tcagctgcct gttctcttct
    45181 gtaagagatt ggtagaattg cccctgctca tagatatgca tctctaaaaa actgtttctg
    45241 aatgcctgaa tattattttc ccttaaatct tgagtcaact gggcaaatct ttcctctaat
    45301 tcttgatttt catccaaacc aatctcacct cgccttattc ttcaaaccgt ctcacaactg
    45361 cctccatgtc tgcggctaaa ggctgcttga actccagcaa ctcttctgta aatggatggt
    45421 aaaaacgcag atcataacaa tgaagggctt gtctttttat tcccagatcc attcgcccgc
    45481 catacatatc atcaccgagc agggcacagc caattgcgga aaaatgaact cttatttgat
    45541 gtgtccttcc tgtatgcagc tggatatcaa ctaacgcgat ttctgttagt cgcttctcta
    45601 tccagtactc agtctgtgcc gaacgtcctt catcagagac cattcgcttc aatagcgaag
    45661 aataatctcg ggcgatcggc aaatcgatat gtccatgtgc cgataaatct gaaagccttc
    45721 cactgactaa ggcctgatat tttttcacaa acttcttttc acgaagttgc atatctaatt
    45781 ttgcgtgcgc aaatccatgc tttgcaaaaa gcatcaaacc agatgtatcc cgatctagtc
    45841 gcgtcaccac atggattact tggttttcgt aaccttgttt tttataataa gcctttacac
    45901 gattagccat tgtcccattt ggatggtatt gggcaggaat agacgaaacg cctgctggtt
    45961 tatttactac cagaacgtgt tcatcttcat acacgatatc gatcggtgtt tcatccaaaa
    46021 gtacggtttc atgttctcct tcagcaggaa tcacaatggt taccttatca tccttagcta
    46081 gagaaaacaa gacattttct acttgatcat taactttgat ttgaccgcct tgaaatttta
    46141 ttttcgctaa cagacccttt gagatccctt tttcttttaa aaaatatttt acttgctgag
    46201 aatgatcttt ctcataatcc catgtaaact gcatatcaat cctcactaat aaaggcatct
    46261 ttcacacgat gccaaaaatg catatgtcga taagaagcaa aatggatccg ttcttctgct
    46321 attcgataat aaattgagcg gatatttgct tttgcgacat tcaattgatc aaccgttaca
    46381 agataatctt ctgtattttc caattttatt tctagccatt catcatgggc aatcacgatc
    46441 ggagaaccca aagtacgaaa cactcgatta ttcagtgaag caatctctgc caactgaaag
    46501 gcattgatac taggatggat cacagcaccg ccgacacttt tattatatgc tgtcgaacca
    46561 gtaggcgtag aaatcgtcaa tccatctcct ctaaaacttt caaacagctc gtttttgatg
    46621 tatacatcgg ctaccatcgt acgattggcc cgtttaatcg ttgattcatt cagtgctaaa
    46681 aaatgctggt ccggtgtttc atccaaataa ctgatccgaa catccagcaa gggataacta
    46741 acactttgtt cccggttcgt tcgtaagctg tctactagtt cttccagctc gtaatcccgc
    46801 caatccgtgt aaaaccctaa atgacctgta tgcacgccca agaaacgcac atcattcaga
    46861 caatgactgt agtgatggaa cgccgacaat aatgtgccgt ctccgccgac tgagatgacc
    46921 aattctggct catgatcatc tcgttgaatc cctgcttgtt ctaaaagtgc gttcaaacgt
    46981 tctgtcactt ctatagtctt tgcttcttta ttatgaacga tcgctacttt catcccctgc
    47041 ctcctctctt tcgctttctt tcttcaaatg cgttcgatag tagacatcat ctgattttcc
    47101 tcttccatgg gagaataacc gctgtgcttc ttggatttct tcacggatac tggacatctc
    47161 ttcatccaac tgataggcag cttctgccgc acgctgaagt cgttcactca tttcttctgg
    47221 aaaaacgcct tgatatttat aattcagcga atgctcaatc gttgcccaga aattcatcgc
    47281 aagagtccga atctggattt ccgccaaaat tttcttctca ccgctgatca gctgtacagg
    47341 atactcgatc accagatgat aagaacggta gccacttgct tttttatttg tgatgtaatc
    47401 tcgttcttgg agaactttca tgtcttttct tttacggatg atctcaacaa cttgatggat
    47461 atcttctaca aactgacaca tgatccttaa cccggcaata tcagacattt cttcttctaa
    47521 acgtcccatt ggaatatgcc gtaattgtga ttttgttaca atactatcga ctggcttgac
    47581 tctcccagtg acaaattcaa tcgggacatg tcgattttgt tcacggaact gtttgcggat
    47641 cccccgcagt ttcactttca attctgatac agtttgttca tatggtgcta aaaatgcttc
    47701 ccaatctcgt tccatagctg atacctctac ttcattaaca ttcattgttt attctaccat
    47761 atatgcaaag ttttttcgta ctcttgattt atgcattgac caaaaaaaga aaattaggca
    47821 aaatagacaa aaaggagtcg atcaaatgac acaaatgctt gaaatcgaat ataaatctat
    47881 gctgacaaag caagagtacg agatattcat cgctcactat caattaacag ataaagattt
    47941 ccgtgtacag actaatattt atttcgatac cgttgatgaa cagctgaaga aacaaaattg
    48001 tggcctacgg atccgtttcg ttggtcatca agctgaatat acattaaaaa cacctgctga
    48061 aaaagggcgt ctcgaaacaa cggatacctt tataacagaa gaagcagaag catttatctt
    48121 cgaaaaacgt ctgcctcgtt caggcgcagt ttttcaaaaa ctttctgatt tgaatatcga
    48181 tcctgcttca ttaatccaaa ccggaaagct tacaacaaaa cgggctgaat tccctattga
    48241 agaaggactt ttagctattg atgaaagctg gggagacaat ctacacgatt ttgaactgga
    48301 actagaggta ggggatgcat ctgtgggaaa aattgctttt ataaattttt taaaacgttt
    48361 ttctttacct tatcgtcctg ctaagaacaa gatccaacgt atgcttgaag ctaaaaatta
    48421 gaaacctttc attttcacaa ggcacttgag ttcatttttt tacttttttc tgttaagtta
    48481 taggatgtga aagaatctag tcactagttg tatagaatct attttggggg agattcatat
    48541 gatcgaaatc tatttatttg ttaatccttt aggggaatac tgtttcgaaa tggaacaaca
    48601 gttattacaa tttatcgaag aagagtacgg gacaccatca aaagaaaaga tgcaatttcg
    48661 ctttcttcct ttggtcaatt tgcagacaat tggggatgtc atgcaacaaa aaggaatttc
    48721 tcagaacaat cttgaggaac gtaatcactt attctcaatg acttattctg cagcattgga
    48781 ctgcaaagcg gcgcagtttc aaggaaagaa aaaaggccgc cgttttttga tcaaattgca
    48841 agaagcagtc ggctgtaatg acataccata ttctcaagaa cttgctgaat ccatatttga
    48901 agaaatcggc ggcgatttga atatgtttaa agaagatcgt cagtctgact tcgttaaaga
    48961 agtattcttt tctgatcaaa aaattgctcg ggaaatgggc atcacaaaac acccttctgc
    49021 agtcgtttac aattatgcct gcgaccgtga ttttggtgtt ttagtagaag taaaaggtac
    49081 aagtagcctt tggagaatca cagagctgtg taaaacaaca gataactcat atcaagtttt
    49141 taatggtgaa catttacata caaatgattc tcagttccat tcaagaacta gccatttaca
    49201 cttgctttct aattaaaaaa atacgtcgac tcccttacaa gagagccgac gtattttttt
    49261 cgtttctatt cgagaaaatc aaaaatagaa agccttataa acgatcgatc aattcttcta
    49321 attcatttag acgttgttca aacatcgaca ttgcatcttc aatatatgca gcctgtgtca
    49381 tatcaactcc tgcttttttc attacttcca caggataatc gctgttacct gcttttaggt
    49441 aagccaagta gttctctagt gcctctggtt cctggtttag tattttcttc gccagtgctg
    49501 aagcagcaga aaagccagtc gagtattgga aaacatagta attataataa aaatgcggta
    49561 tccgtgacca ttcgaattta atttctgggt cttcttcgac tgctggacca taatattttg
    49621 cattcaattt accataacta tcacttaggt attcactagt caatggcaca cctttttcat
    49681 cttccgtatg catgaaatgc tcgaattcag cgaattgtgt ctggcggaaa actgttcctt
    49741 taaacccatc caagtaatgg ttgagtacat aagcccgtac acgagggtct ttttctgttt
    49801 ccaataaata ctccgttagg atattttcat tcgttgtcga agcaatttct gctaaaaaga
    49861 tggagtagtc gccgtacaca taaggctggt tcgaacgagt gaaataacta tgaacactat
    49921 gtcccatttc gtggacaagc gtaaatagct gatccagcgt atcatgccaa ttcaataaaa
    49981 tatatggatt tgtgtcatag cttcccgaag aataagcacc gcttcgtttc cctttatttt
    50041 cgacaacatc gatccaacgt tcagagaatg ctttttctac gatggccatg tattcttcac
    50101 ccattggttt cagtgcttct aaagcttttt cttttgcttc ttcgtacgta aaggtaattg
    50161 gagcttcccc taagaccggt gtataaagat catacatgtg cagtttttcc acttctaata
    50221 aacgtttccg taattccatg tatcgatgta acaaaggcaa atgcttgttt accacgtcta
    50281 ccaaagtatc gtatacactt tcaggaatat gattattgct caaagacgct tctctggcag
    50341 agctgtaatt acggactttc gctttaaaat tatgtccttt tatatgtgtg cctaaagtag
    50401 aagcaaatgt atttctaaat tgttcgtaaa cactgtacaa tcccttaaat gctgcttcgc
    50461 gcacccttcg atccgtgctt tctagcaact gaccatacac gccatgagat aattggacta
    50521 tttcaccatt ttctccttca atcgttggaa aaactagatc tgcattattc aaaacagcaa
    50581 atgtatcact tgaagcatca aagatttcac ctgctccagc aagaagagat tcctgctcag
    50641 cagataagac atgggctcga ttatctacta tttgctggat ataatggcga tagacttcca
    50701 attttggttc ttctttgaaa tattgccaaa tctggtcatc tgacaattgc aatatttctg
    50761 gttcaaacca ggaaacggct tcactaactt tcgaaaacag actgcttgct cttgcataaa
    50821 gcgcttggta atctgtattt ccagtgtctt gatcgttttt aaggtgcgca tatacataaa
    50881 tgacttcagt ttggcgataa accctcaata cgaattcaat cgcatttaaa aattgagaag
    50941 cgccttgatc aagtgttcct ttgtgttttt cagattgttt taactcttct gataattcca
    51001 aatatttctc atcaaactct tggtcgcttg aaaagatctt ggtcaagtcc caagttaaat
    51061 tttcaggcaa ttcttctcgt tttggcaact gctttacttc catgaaatcc actccttttc
    51121 ttcccttagc tctatctatc aaaatagatc atagtctaac atccacatgt cacagcatct
    51181 aacttttcta ctcattcatg tgtctgcttc catcaatttt agcataacta aatcttgttt
    51241 tacatagaaa accgattcct agcctatttt tatacttact attttttcaa agaagacaag
    51301 tgtatgcatt cgttaaaaaa ctgctgatag accatctctt catcaatcat aggaaaaagc
    51361 cattcccttt tatgttcttt tatttggcaa agccatgagt tgaaatcagg aactttctgg
    51421 ttttccaagg cttcttcgta caacattcga taaagaaaaa cctgttcttg taaataaaag
    51481 aaaaaccgac ttttttgata gatccacgat gatagatgca aaagatgttt atgacgcaag
    51541 tagcattgtt cttgtatgga tactgtattc ttttgcttgt gaataagttg gcgtgtcagc
    51601 catttcttcc catcttcact cactttttct atttttttca tttggttcat tttaatttcg
    51661 ttactagtga atatttcttt aaggtcccta ctagaaaaag tccagcagct actgcaaaag
    51721 gaaatatgcc ctgatacagt ctctgtaata tgatgataca aataaagctt ttgtttcttg
    51781 agatcagctt tccagcaatg cactcctctt tttctattat agtagcagca acttttttct
    51841 atcaatgaga attgcgattg tccaagaaaa gaattaccaa gtatccacca tacttggtaa
    51901 ctgttattca aataattttg ggttctttca ataaatctct ggtgagacag tctagaacat
    51961 tggatttcta ttgctagatt cgccaacaaa atatccggcc tttgccgcaa cctcggcaaa
    52021 taggcttcca aaaatactgg ttctactgac tgtcccagcc aatccatgaa gcattccttt
    52081 aactgtaagt gctcttttga ttctctttca ctaaagaccg aacagttatc tgatttttga
    52141 tgggcaaaat gagcacattt tttctttccc tttttcaaaa tcactctttt tcgacagcca
    52201 cgacaataat atttctttcc tttttctgca tgtttagcaa aacagcactt attatattca
    52261 ttcattgcaa gcagcatttt tctccctctg aacttattta cgtctaagaa tttatttaca
    52321 cgaaaaaaga gggcgtgaaa aagctattct gcatcaagta ataagaacag ctaaacaaaa
    52381 atagttccca atatttttcg tttggttggg cttgttaccg cagatgcaag cttttgaaca
    52441 catttatacg aaaaaaagac tgcgacacaa tttttgtcac agtctcttac tataatgctg
    52501 atttatacaa aataaaattt cgttcgctcg atagcattac gcgccatcag acattgtcca
    52561 tattcattca atgtttctgg tgtacgatcg ctcttttcag caaactccaa aattcgagca
    52621 atctgattct ctacatccgc ttggttgaga ttttccatcc agaagtgaac gactaggtaa
    52681 tagcgctctt cgaataaata caaatcagtc caagcagact ctagttcgac atctgttgca
    52741 agttggatca cttgttcaaa atcttcaaaa gcaaaagttg cttgtctttc ctgttcttct
    52801 agggcgcttc cagataattc ttcctccgga tcgctgccaa taatctgctg acgaagaaag
    52861 tcactcacat cttctgaatc aacagtagaa tcttctgatt gagaaatttc ttcatccgga
    52921 agatttttac tgataaacag ttctaatccg tctccttttg gcaaaacttg gaaagtgaca
    52981 gcttcgcttc ctcggaactc ctcttcgata tcaacttctt ccaaaatact ataaaagaaa
    53041 ttttcgattt ctttgtgatt gccaagtaaa tccaaaaatg aaacgccccg ttcctctaaa
    53101 tcttcatgac caattagtac acggatcgta ttttcattaa tatgttccat ttccattttg
    53161 ctacacctca ctttgccaca atgtttaata tagattcatt gtagcgaaat cagcttaaat
    53221 gtaaagaaaa aaaatctcgc tgttaagcga atttaataaa accagcggat ttttcaacct
    53281 ttcaagctat cttatttaac tataacattt ttggcagacg aatactatat agccgacttt
    53341 tcagctaatt ttccctctct atttttcgtg caatcatcat cttctactac ctttttatac
    53401 atctaatcaa gaatactttt tgtaaaaaca aaaaaacttg gaaaaactat ctttgtaata
    53461 gt
//
LOCUS       DACEPD010000011        52906 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.011, whole genome shotgun sequence.
ACCESSION   DACEPD010000011 DACEPD010000000
VERSION     DACEPD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.011"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(233..1081)
                     /locus_tag="IW797_000606"
     CDS             complement(233..1081)
                     /locus_tag="IW797_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1223401.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IW797_000607"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IW797_000607"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ1223402.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="IW797_000608"
     CDS             complement(3582..3761)
                     /locus_tag="IW797_000608"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ1223403.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IW797_000609"
     CDS             complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IW797_000609"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1223404.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..8188
                     /locus_tag="IW797_000610"
     CDS             5909..8188
                     /locus_tag="IW797_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1223405.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8281..8928)
                     /locus_tag="IW797_000611"
     CDS             complement(8281..8928)
                     /locus_tag="IW797_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223406.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9107..9826)
                     /locus_tag="IW797_000612"
     CDS             complement(9107..9826)
                     /locus_tag="IW797_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1223407.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9957..11108)
                     /locus_tag="IW797_000613"
     CDS             complement(9957..11108)
                     /locus_tag="IW797_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ1223408.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11325..12065)
                     /locus_tag="IW797_000614"
     CDS             complement(11325..12065)
                     /locus_tag="IW797_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1223409.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IW797_000615"
     CDS             complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IW797_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ1223410.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12449..13042)
                     /locus_tag="IW797_000616"
     CDS             complement(12449..13042)
                     /locus_tag="IW797_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1223411.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13026..13673)
                     /locus_tag="IW797_000617"
     CDS             complement(13026..13673)
                     /locus_tag="IW797_000617"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ1223412.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IW797_000618"
     CDS             complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IW797_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ1223413.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IW797_000619"
     CDS             complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IW797_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ1223414.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15119..15646)
                     /locus_tag="IW797_000620"
     CDS             complement(15119..15646)
                     /locus_tag="IW797_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ1223415.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15826..16611)
                     /locus_tag="IW797_000621"
     CDS             complement(15826..16611)
                     /locus_tag="IW797_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ1223416.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16604..17473)
                     /locus_tag="IW797_000622"
     CDS             complement(16604..17473)
                     /locus_tag="IW797_000622"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ1223417.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17474..18853)
                     /locus_tag="IW797_000623"
     CDS             complement(17474..18853)
                     /locus_tag="IW797_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ1223418.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IW797_000624"
     CDS             complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IW797_000624"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1223419.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKTSAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IW797_000625"
     CDS             complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IW797_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ1223420.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IW797_000626"
     CDS             complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IW797_000626"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ1223421.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IW797_000627"
     CDS             complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IW797_000627"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ1223422.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IW797_000628"
     CDS             complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IW797_000628"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1223423.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22811..23035)
                     /locus_tag="IW797_000629"
     CDS             complement(22811..23035)
                     /locus_tag="IW797_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1223424.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23088..24050)
                     /locus_tag="IW797_000630"
     CDS             complement(23088..24050)
                     /locus_tag="IW797_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ1223425.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24060..24500)
                     /locus_tag="IW797_000631"
     CDS             complement(24060..24500)
                     /locus_tag="IW797_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1223426.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24806..25771
                     /locus_tag="IW797_000632"
     CDS             24806..25771
                     /locus_tag="IW797_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1223427.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25828..26058)
                     /locus_tag="IW797_000633"
     CDS             complement(25828..26058)
                     /locus_tag="IW797_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ1223428.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26144..27031)
                     /locus_tag="IW797_000634"
     CDS             complement(26144..27031)
                     /locus_tag="IW797_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ1223429.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27120..28343)
                     /locus_tag="IW797_000635"
     CDS             complement(27120..28343)
                     /locus_tag="IW797_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1223430.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28360..29439)
                     /locus_tag="IW797_000636"
     CDS             complement(28360..29439)
                     /locus_tag="IW797_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1223431.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="IW797_000637"
     CDS             complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="IW797_000637"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ1223432.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31297..31563)
                     /locus_tag="IW797_000638"
     CDS             complement(31297..31563)
                     /locus_tag="IW797_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ1223433.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32042..34276
                     /locus_tag="IW797_000639"
     CDS             32042..34276
                     /locus_tag="IW797_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ1223434.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34424..34744
                     /locus_tag="IW797_000640"
     CDS             34424..34744
                     /locus_tag="IW797_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ1223435.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34949..35173)
                     /locus_tag="IW797_000641"
     CDS             complement(34949..35173)
                     /locus_tag="IW797_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ1223436.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35371..36951)
                     /locus_tag="IW797_000642"
     CDS             complement(35371..36951)
                     /locus_tag="IW797_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ1223437.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37352..38728)
                     /locus_tag="IW797_000643"
     CDS             complement(37352..38728)
                     /locus_tag="IW797_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1223438.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38856..39974)
                     /locus_tag="IW797_000644"
     CDS             complement(38856..39974)
                     /locus_tag="IW797_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ1223439.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            40116..40550
                     /locus_tag="IW797_000645"
     CDS             40116..40550
                     /locus_tag="IW797_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1223440.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(40628..41161)
                     /locus_tag="IW797_000646"
     CDS             complement(40628..41161)
                     /locus_tag="IW797_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ1223441.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(41255..42433)
                     /gene="mutY"
                     /locus_tag="IW797_000647"
     CDS             complement(41255..42433)
                     /gene="mutY"
                     /locus_tag="IW797_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ1223442.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42426..43223)
                     /gene="recX"
                     /locus_tag="IW797_000648"
     CDS             complement(42426..43223)
                     /gene="recX"
                     /locus_tag="IW797_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ1223443.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43330..44712
                     /gene="rlmD"
                     /locus_tag="IW797_000649"
     CDS             43330..44712
                     /gene="rlmD"
                     /locus_tag="IW797_000649"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ1223444.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44886..45464)
                     /locus_tag="IW797_000650"
     CDS             complement(44886..45464)
                     /locus_tag="IW797_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ1223445.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45786..46256
                     /locus_tag="IW797_000651"
     CDS             45786..46256
                     /locus_tag="IW797_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ1223446.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(46364..47590)
                     /locus_tag="IW797_000652"
     CDS             complement(46364..47590)
                     /locus_tag="IW797_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ1223447.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(48344..48544)
                     /locus_tag="IW797_000653"
     CDS             complement(48344..48544)
                     /locus_tag="IW797_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1223448.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(48678..49286)
                     /locus_tag="IW797_000654"
     CDS             complement(48678..49286)
                     /locus_tag="IW797_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ1223449.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(49371..49841)
                     /locus_tag="IW797_000655"
     CDS             complement(49371..49841)
                     /locus_tag="IW797_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ1223450.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(49889..50803)
                     /locus_tag="IW797_000656"
     CDS             complement(49889..50803)
                     /locus_tag="IW797_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ1223451.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            50949..51752
                     /locus_tag="IW797_000657"
     CDS             50949..51752
                     /locus_tag="IW797_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ1223452.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            51930..52877
                     /locus_tag="IW797_000658"
     CDS             51930..52877
                     /locus_tag="IW797_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1223453.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT        15286 a        11143 c         9028 g        17449 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     7021 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8221 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8281 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8341 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8401 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8461 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8521 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8581 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8641 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8701 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8761 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8821 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8881 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8941 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     9001 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     9061 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     9121 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9181 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9241 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9301 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9361 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9421 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9481 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9541 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9601 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9661 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9721 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9781 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9841 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9901 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9961 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
    10021 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
    10081 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
    10141 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10201 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10261 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10321 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10381 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10441 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10501 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10561 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10621 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10681 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10741 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10801 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10861 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10921 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10981 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    11041 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    11101 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    11161 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11221 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11281 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11341 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11401 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11461 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11521 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11581 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11641 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11701 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11761 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11821 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11881 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11941 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    12001 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    12061 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    12121 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12181 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12241 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12301 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12361 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12421 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12481 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12541 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12601 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12661 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12721 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12781 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12841 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12901 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12961 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    13021 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    13081 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    13141 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13201 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13261 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13321 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13381 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13441 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13501 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13561 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13621 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13681 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13741 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13801 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13861 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13921 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13981 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    14041 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    14101 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    14161 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14221 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14281 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14341 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14401 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14461 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14521 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14581 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14641 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14701 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14761 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14821 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14881 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14941 tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa acaacgctga
    15001 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    15061 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    15121 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15181 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15241 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15301 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15361 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15421 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15481 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15541 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15601 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15661 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15721 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15781 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15841 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15901 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15961 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    16021 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    16081 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    16141 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16201 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16261 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16321 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16381 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16441 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16501 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16561 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16621 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16681 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16741 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16801 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16861 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16921 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16981 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    17041 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    17101 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    17161 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17221 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17281 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17341 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17401 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17461 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17521 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17581 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17641 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17701 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17761 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17821 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17881 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17941 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    18001 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    18061 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    18121 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18181 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18241 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18301 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18361 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18421 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18481 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18541 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18601 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18661 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18721 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18781 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18841 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18901 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagttttcac tttcaatatc
    18961 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    19021 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    19081 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    19141 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19201 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19261 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19321 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19381 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19441 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19501 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19561 tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac attgactggc
    19621 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19681 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19741 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19801 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19861 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19921 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19981 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    20041 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    20101 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    20161 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20221 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20281 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20341 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20401 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20461 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20521 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20581 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20641 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20701 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20761 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20821 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20881 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20941 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    21001 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    21061 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    21121 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21181 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21241 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21301 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21361 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21421 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21481 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21541 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21601 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21661 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21721 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21781 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21841 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21901 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21961 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    22021 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    22081 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    22141 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22201 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22261 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22321 cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt ttgctactaa
    22381 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22441 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22501 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22561 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22621 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22681 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22741 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22801 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22861 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22921 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22981 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    23041 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    23101 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    23161 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23221 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23281 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23341 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23401 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23461 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23521 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23581 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23641 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23701 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23761 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23821 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23881 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23941 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    24001 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    24061 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    24121 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24181 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24241 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24301 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24361 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24421 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24481 gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24541 gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac
    24601 tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa
    24661 tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt attttctgtg
    24721 aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt atagtctttt
    24781 tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc tccccatgct
    24841 gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa
    24901 acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt caaaaaaagt
    24961 attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca cggaattaga
    25021 tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata accgacaaat
    25081 gaattatttc agtaattata attatgtaca tattaccaat aagggatttg tattaacaga
    25141 tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc cgtctatttt
    25201 gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt tgttagcttt
    25261 gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa aggaactcca
    25321 agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa gacaacaaca
    25381 tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata tcgaattagt
    25441 tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac catcgctcac
    25501 cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg tttgttatgg
    25561 tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct attatgaaaa
    25621 gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca
    25681 aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg aactgttaaa
    25741 caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt aggcatcttc
    25801 aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt ccatcgctac
    25861 gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta attcaaatgt
    25921 atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt caaaacggcg
    25981 aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta tgattgcgac
    26041 taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa
    26101 gcatagccta cttgcattga ttttctctaa aaaaactcgt ttttcatgat tttttttctg
    26161 gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca ttctcaaaac
    26221 ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc aatacaagat
    26281 ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa
    26341 aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa gaccagttag
    26401 ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg tttttttgtg
    26461 catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc tgattagcca
    26521 tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta ccaaattctt
    26581 tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg atcgtcagtg
    26641 taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca tctgcgcctt
    26701 tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta gtcaaatcaa
    26761 ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag tagatttctt
    26821 ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct gctaccgctt
    26881 ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt gcttttgtcc
    26941 gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca
    27001 tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc
    27061 ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat gtttgaattt
    27121 tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc ttcttctttt
    27181 cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat catagcttcg
    27241 ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg atctttagga
    27301 acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt
    27361 ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc cacgacacaa
    27421 ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga agtagaagca
    27481 ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc cacttctcct
    27541 gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa ctcttctaca
    27601 taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat gacctgagga
    27661 aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa agaaagaatc
    27721 atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc ctgctgagtg
    27781 atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct
    27841 attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac tacacctgtc
    27901 gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat cacttttcct
    27961 ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat cagaggtatt
    28021 ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt atgggtatga
    28081 ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc tctaacaaca
    28141 actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac atctttttcc
    28201 aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc atgtctttct
    28261 aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg aaagtaggta
    28321 tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga cctgatttac
    28381 tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat ttttaagttc
    28441 ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt gtgtcgtact
    28501 cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt ttgtcttccc
    28561 catatagcag ttcttgctat cttctaacca gccattatac actggaatat ctcgaacaag
    28621 tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct cttcataaga
    28681 agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc cacgaatgta
    28741 accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga tgttccgagg
    28801 aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca tcttcttagc
    28861 aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac aaatgattac
    28921 tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca ttggatcagg
    28981 gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac ggataccata
    29041 gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg ctttttggta
    29101 caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatccga taaaggaatt
    29161 tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt ctcttctggc
    29221 tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga tgtcataatc
    29281 ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt tttgatgatc
    29341 aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat ataataatac
    29401 cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac tgtcactcaa
    29461 acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa aaagaacaaa
    29521 aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt tattttacat
    29581 attcatgaac aagttcaacg acttcttcca tcatatcgca ttctttcaat gcacgatcag
    29641 caagttcagc cattttcgct gtgtccaagc gtttcattaa actacgcgtt ttaagaatag
    29701 atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga acagctgttt
    29761 gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt gctgcatcaa
    29821 ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag taagaaacgc
    29881 gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta cctacagaga
    29941 agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga atctcgatca
    30001 tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat ttttgttttt
    30061 cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc atagggaaca
    30121 tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt gtacggaaca
    30181 tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag aatggattca
    30241 tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc attgtacgaa
    30301 caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca tattggtcat
    30361 cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac agaccaactg
    30421 cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg tttgcagcta
    30481 attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt tcccattctg
    30541 ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt tcatctgtag
    30601 gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct tctttgactt
    30661 tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt gccatgatag
    30721 cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa ttgcgatcta
    30781 attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct tcgttgatca
    30841 ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta gcaacgtcac
    30901 gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg aacataccga
    30961 tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg tctttaatat
    31021 tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg gcatcaaata
    31081 cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc tgcaattctt
    31141 cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta tcttctactg
    31201 ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca acggctacac
    31261 cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc cttctttctt
    31321 cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg cagtaatagt
    31381 aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt tcaagtttac
    31441 tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg tttgtactaa
    31501 tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt ctttcttttc
    31561 catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt cttttttagt
    31621 aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca ttttttttcg
    31681 ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt ccggattctt
    31741 tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc ttgtcgcttt
    31801 ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat atgaaacgaa
    31861 ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga atggtcaata
    31921 gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat tcttttacct
    31981 catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac gtgaatgatt
    32041 catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa
    32101 tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca aaaatcaaca
    32161 aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca gtttggacga
    32221 tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc aaacaccacc
    32281 aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag gcaatacaga
    32341 aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag gtgatatcga
    32401 tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa atcgccgaac
    32461 caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag ttgttgaagg
    32521 tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca aagaagtcat
    32581 tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat ttgaagaacg
    32641 tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat tcatagatga
    32701 agttcacgaa atcgtcggtg ctggttctgc tggtgatgga aatatggatg ctggtaatat
    32761 tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta ccttaaatga
    32821 gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg tacgggtaga
    32881 tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga
    32941 ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt tatccaatcg
    33001 ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg aaacaggttc
    33061 taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga
    33121 agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag cagcttatta
    33181 tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac
    33241 acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag gtattccagt
    33301 gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg acttgaaagc
    33361 tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg
    33421 tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg gaccaactgg
    33481 tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt cacaagattc
    33541 aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta aattgattgg
    33601 ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa aagtacggag
    33661 aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag atgttttgca
    33721 tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa gaacggtcag
    33781 ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag tagaagcaaa
    33841 cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc aattaaacaa
    33901 ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca gtgcattatc
    33961 aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc aaatgctggc
    34021 agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag ttgacttagg
    34081 ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga
    34141 tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag caaaactgga
    34201 taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag aatcagccga
    34261 agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg agaaatgccg
    34321 tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag aaattataat
    34381 aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat tggtaaatgt
    34441 aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg cccatctagt
    34501 caaggtctac acagccggaa ataccactat tgtctacact gaagcccctg aacataatga
    34561 aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa
    34621 ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca aagtaatcga
    34681 attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta cattagccgt
    34741 gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaagtaaag aacacttttg
    34801 tctcacgctc taaacacgta taaacggcga aagcagaagc aactccttcg gaaataagct
    34861 gaaattcacg aaaattgctt ttcaaatttt tgtgaattcc agcttatttc ttggagttaa
    34921 acgcttctgt ctcagcctct ttttctttct agctttttga tttttctctt ttcttttgta
    34981 cttttctctt ttcattttca gttctacgcg tgtctttatt tttaactttt tcctctttat
    35041 ggatgattga tcgtactttt tgtgcaattt gctcgttttt gtcccagtct tttacatcta
    35101 ataatcgaca taatcgtaac atctcttctt ttttctgttt ttttctttca cgtctttgcc
    35161 attctgtttc catgtgtcac actctccttt ctttaatcat atgtacgcaa aataattttt
    35221 atattttttt gattagacat ttctatgatt ttaatatagt aaaaagagcc gtcacggaaa
    35281 atccgtcacg gctctaaaca aaatatgaga ggctgttttt gtttggctat tcttattact
    35341 tgatgcaggg cagcttgtgt caagacttct ttacatcaaa ctttttaatt ttacattggg
    35401 atatttatcc gcaaaccagc gttctgcaaa ttggttctcg aataagaaca acggttggtc
    35461 gaaacggtca cgagccaaga tattgcgact tgaactcatc ttctcatcca aatcttctgg
    35521 atcgatccag cgagcgattt tgtgtcccat tggagacatg attacttcgg cgttatactc
    35581 atttaacata cgatattgga atacttcgaa ttgtagctga ccaacagctc caatgatata
    35641 ttcttccgtt agatatgttt tataaagctg aatcgctcct tcttgaacta attgatagat
    35701 tcctttgtgg aaggattttt gtttcatgac attttttgca gtcactttca taaacagctc
    35761 tggagtaaat gaagggagtt cttcataagc aacttttagt tttccttcga acaatgtatc
    35821 cccaatctga taattgcctg tatcatatac accgatgata tcgcctgcta ctgctttttc
    35881 gacgttttct ctcgaatctg ccataaattg tgtgacattg cttaatttca gttttttatt
    35941 tgtacgttcc aaccagacat ccattcctcg ttcgaatgtt ccagaacaga ttcggacaaa
    36001 agcgattcgg tcacgatggg ctggattcat atttgcttgg atcttgaata cgaaacctga
    36061 aaattcttcc tcataaggac ttaccatctg atcatcttgt gttttgtgtc cataaggagc
    36121 tggtgccaat tcaacgaaag tctctaaaaa agtctgtaca ccgaagttag tcaatgcaga
    36181 accaaaaaat actggtgtct gatcgccacg agcaattttt tcttgattaa atgcatctcc
    36241 agcctctttg attagttcta cttcttctaa gacttgttca tataaggaat tttcagttag
    36301 aggatgattt tcgggaagca gtccatcttc atccaattct actaatcgtt catcctgata
    36361 attttccgga cgataaaact ctacacgatt atggtagata tcataaagac cttctagccc
    36421 tttacccata ccgattggcc aattcatcgg ataagattcg atatctaata gctcttctaa
    36481 ttcttctaac aattccaatg gttcacgacc atcacgatct agtttgttga taaaagtaaa
    36541 tatcgggatg ccacgttttt ttacaacttg gaataatttc tttgtctgtg cttcgatccc
    36601 ttttgcacta tcgattacca tgaccgcact atccacggcc atcaaagtac gataagtatc
    36661 ttcagaaaaa tcttcatgtc ctggtgtatc caagatattt actcgtttcc cttgataatc
    36721 aaactgcata acagaactag taacagaaat cccacgttgt ttctcgattt ccatccagtc
    36781 tgatttggca aaattacctg tttttttgcc ttttacggtc ccggcttgac ggatagcccc
    36841 accaaacaat agtaattgtt ctgtaatcgt tgttttccca gcatccggat gggaaatgat
    36901 cgcaaaagtt ctgcgattgt ccacttgttg ctttaatttt ggattattca taacctaact
    36961 ccatttcttc cattcaatta tttatatttt ctctaggcac agattgtccg atctcttaaa
    37021 gaaagacaat ttcatatagg gctgtgacaa aagccgtgta gcagtctctt tttccgagta
    37081 aacggcgttc tagagacagc ctatcagcaa taagcccacc caagccaaaa atacgaaaag
    37141 ctgtttttgc ttggctgctc ttattactta atgcaggata gctttttcac aacctttgtc
    37201 aactactaca actgaatcag tatagcatgg attcagcgaa tgaaaaagcc accaaaccat
    37261 aagagtcaaa tatttttttg tcagctgact aaaagaaaag acgtgttgct tgtttattca
    37321 agcaacacgt ctttcataac caagtttcca attatcttct tggctcgtca tcttcgaatt
    37381 cttttctgaa gtatcttcgt tcttcttcag gttccgcttc tgtttcttcc ggatcatgaa
    37441 aaatgacacg gattttaagg attcgagaac cttccatttc ttcagaaacc agcgtaatat
    37501 tatcgacatc aaacgataac ttttcgcctt catccggaat catgcctaat gccgtaatca
    37561 aataaccagc catcgtatct acatcactca tatgcagatc actgtcaaaa gcttcattga
    37621 actcatcaat cagcatacgt ccttggatga tatattcgtg ttcatcgatt ttttcataaa
    37681 gattctcaac ttcatctgat tcatcatcga tttctccgac gatttcttct aaaaggtctt
    37741 ctagtgttgc taacccaacg acgccgccat attcatctaa taaaatagcc atctgattct
    37801 gcgtcttttt catttcatat aacagatcat caataaagac cgtttcagga acgaataaag
    37861 gctcctgcat gattttcttg atatctaaat tatcaaagcc gaatttatgg gcagctttta
    37921 gtaaattctt tgtatgaagg attccgacta ctttatcttt gtcttcgtta taaacaggga
    37981 ttcttgaata gttttcggat aaaacttcat tcacattttc ttccacagtg tcgttgatat
    38041 caatcataaa tgcatccgtt cgtgggacca tgacttctcg ggcaactttc gtatcaagag
    38101 aaaaaacccc ttggagcatt tctaattctt cattattcaa tacgccttcg ttttcaagca
    38161 tataacgcat ttcatctctg gtcatttttg aatcttcatc atcaaaagtc atcggcgtga
    38221 tacgcgccaa taaacttgtg gaagcagaaa gcagccagac gaagggctta gctactacac
    38281 caatcaagcg tatcagccct gaagtgaact ttgcgacttc ttctgattta tttaaagcaa
    38341 tacgtttagg gtacaattct ccaaaaacaa tcgatacata ggtcaaaata gccagcacaa
    38401 tgatatttgc aatatttttt gccgctgtgc cgccacctaa taccggagct aaccgttccg
    38461 ataaagtatt agctagtgaa gcaccagaca aaatgttgac caaagtgatt cctacttgga
    38521 ttgtcgatag gaaattattt ggattctgca atatttttaa aagcttttga gatttcacat
    38581 ctccttcatc agctttttgc tcgattcgat tcttattgat cgaaacaaca gcaatttcgg
    38641 aagctgctaa aaaagcattg atcaatgtca aaacgatcaa taataaaatc tgcgctaaca
    38701 gcgactgact ctcagggtca gcattcataa tcgcattctc tccttataaa attaattttt
    38761 agtttacgat agaaaaaaac gtggcactat agccacgtta aaatggtatc acaaagcaat
    38821 tttctttgca ataaaaacaa gtgtactaaa actacctacg aggtcgtagc aattttcttc
    38881 ttgttttcat tttgcttgtc ttctttgacg attttgatta tataggaaat gatcgtcttg
    38941 cagattgcgt aaaaaggaac acctaagaag ataccaagca atccggcaat atttccagct
    39001 actagaagaa cgataatgat cgttaatgga tggattttca gtgatttccc aattacatta
    39061 ggataaatga tattcccatc caactgttgg acgattagca caaccacaca acaaagtaat
    39121 gcttttaccg gatcattgaa gactgttact agaaatgcag gtgctagtcc tagataaggt
    39181 cctaaatatg gaattaggtt tgtcaatccc gcaatgaccc caaataagaa agcataatcc
    39241 acaccaatta ccatatatcc taaaaatgtg aaggtaccaa cgaataaaca ttcgattgct
    39301 tgtccgctaa tgtaattagc caatgtttta ttcagttgcc ccaataactc cacaatttgg
    39361 tttcttcttt tttctgggaa aaactgttgg atgtttggca ctagtttttc tccgtctttc
    39421 aacatataga acaagatgaa tggtgctgta atgattacta cggttgccga agcgatcgtt
    39481 ccaacgatcg aacctaaact attggatact ccactaagga attgctgaat gattgtcccg
    39541 taagaaatgt ctaattgctc aaaatattta ttgatatcta cttccttgaa caaaggcaat
    39601 tcggctacct cttttaacca gctttctacc tgtttgatga aatcaggcat attagatgcc
    39661 aatgaggaaa tttgctgtac tagacttggg atgacactaa gtaaaatcca aataattgct
    39721 gcaactaaaa ggagcagcac aattgcgaca gcccatattc ttttgatttt tgctttcatc
    39781 aacagcttga caataggatt caataaatag taaagaaagc ctgcaatcag tacaggtgca
    39841 aataacgtgg aaaaaaatgt cccgatcggt tcgaaaataa agtttatttc tgatgaaatc
    39901 atgatcaatg cagctatgat caataattca agcgaccaaa acattagttt agactgtcta
    39961 agtttttcaa acaatagatt tcctcctttc tttcttacta gtatatatta tattcgttaa
    40021 aaattctttt attctttttt tatttttgaa aacttgccat actttttatt tgttttattt
    40081 tccagtatac tatacgaaag gaaggtggct agaccatgaa agttgtacaa acaaaagata
    40141 caatgagcaa tatttactta gatgctgtac ggatcaggca tcaagttttt gtagtcgaac
    40201 agggtgtgcc tttgtctcga gaaatagaca aagatgaagc ccattgtatc cacttcgtcc
    40261 tgtactcaga taaaaaagaa ccgcaaggaa ctgtacgtct tttgccttta gaaaacggaa
    40321 aaatgaaatt acagcggatg gcgattcttt ctgaatacag acaccaaggg ttaggaaaaa
    40381 tattgattga agaagcagaa aactttgcta agaatcaagg ctataatacg attttattag
    40441 gggcacagtc cacagctgaa actttttacg aaaaactagg atatacagct tacggcgatc
    40501 cttttgaaga tgctggcatg cctcatattt atatgaaaaa gactttataa aaatttattt
    40561 cgaaaaaagc aaaaaacagc aagcttttga gaaaaatctc tcactggctt actgttcttt
    40621 tttgtgttta cttacgcgac aattgcttgt aacggttata ccaaatatca atatagcctt
    40681 ccgagaacgg gccatctcca ttattgatcc agtcaaccag aatcttcaca ttttctttta
    40741 ggatgaagtc aatatcgttc gggtaattca tcattttgct gtgcatctca tattcatcga
    40801 catccaatag tcgtttctcg ccgtcaggaa ataccttgat atccaagtca tagtcgatat
    40861 atttcaaggc ctcatcgtcc agcaaaaaag gtgaagctag attacaataa taggaaaccc
    40921 ccttttctct gatcattgct attacattga accagtattt tttatgaaaa taaacaatcg
    40981 ctggttcgcg cgttacccat cggcgtccat cagattcagt gactaatgtg tgatcattca
    41041 cgccgatcat agagtgttcg cttgttttta ataccatggt atctcgccac gttcgatgca
    41101 atttgccgtc gtgcttgtaa ctttggatcg tcacaaactc tccttctttt ggaactccca
    41161 tagcaaccct tccttaagca gcattttgtg ctttgtgttt gggtgttcat tacaccgaca
    41221 tcataattat tatatcaata aaaatgagct ttgcctagaa aggattccta ttgtttcgat
    41281 ttttctctaa ctgatctacc agtttcattt ggaccttagg aaaaacgacc gaatcaaatg
    41341 catcaggctt caaccacttc gaagttttgt tctcggtaaa ttcttgttct gctccctcgg
    41401 ttgcccgtcc gtaaaaaagg agaacatgcc atttcagatg actaaaaata tgagtaactt
    41461 ctcctaaatg tcgtgtctgc caaacaaaga gttcctgctt ttcaaatggg agattttgct
    41521 cttcttctgc tactaggtca tcaaacagat cgatttctgg ctttgtttcc cattcttttt
    41581 tcaatcgctc atactcctct tgtgtcactt ccatcattgg aaatgtccac atattcgcca
    41641 gcaatttttg gctatctctt tcttcaaaat aatacgctcc agaatgattt tgaagagctg
    41701 cactgatata ataaacgtct tttggctttg ctttttttgt ttttacagga aaactcgttt
    41761 ggattcctcg tttgttagct aaacaaaagg cttggatcgg acaagtctca cactttggag
    41821 aagttggtgt acagatagct gaacctagat ccatcattgc ttggttgaat tcacctggat
    41881 gcttttcatc aatgattttc cgcatcgctt catcaaagat tttcctgcta gaagcttttg
    41941 cgatgtctgc ttcaatgcaa aacaatctgc tgactactcg catcacgttg ccgtcaactg
    42001 caggttctgg aagtccaaat gcgatacttg caatcgcgcc tgtcgtatat ggtcctattc
    42061 ccttcaatga gctaatttct tcaggcgttt gcggcatttc cccatcaaat tctgacatga
    42121 tctgtttagc agcagcttgg atgttccggg ctcttgagta ataaccaagg ccctcccatg
    42181 cttttaaaag cttttcttct ggagcatttg ctagctcttc aattgtagga aaccattcca
    42241 tgaaacgata aaaataatcg atcactgtgt ctactctggt ttgctgaagc atgatctcag
    42301 agatccagat acgataagga tcacgattat agcgccaagg caggtttctt ttttcttgtt
    42361 cataccattg aatgaactga tcttgaaatt cttttgtttc tttatctgtc catttttccc
    42421 attctttatt cactttcctc ttccttttcc gtaatgaatc gctggatcat atccaaatca
    42481 aatccttttt gatataagct ttgctttact tttagatttc tttttttagg ttcaaacctt
    42541 aaatttcttc gccatagttt atctccttgt ttgactaaat aatcatattc tatttcttga
    42601 tctgcttctt gcggaagatc agctatcgct tgttggatca cttcttgaga aaagcctttc
    42661 gtcatcaatg tttgttggat ttttctcaat aattcttttt ggctttttcc gtgatttttt
    42721 ccaaacgctt tttcggctgt ctgatgtgca ttttgaactt ggtcttcaaa tgtatattgg
    42781 ctgagcgctt gttcaatgat ttcaggtttc aaaccttttt gctgcatctg ctgcgctaat
    42841 ttctttggtc ctttgtcact taagcgcata ttcgtgcgca cataactttc cccatagacc
    42901 aaatcatcga caaggtccaa ttcttttaag cgggctacga ttttatttcg atcttcttga
    42961 aggatttcct tttcttttaa gtatgttcgg atttcttttt ccgatcgcaa ctgataactt
    43021 aagtaattca ttgctaggtg aaaaccgaaa tcagctccgc tactcttctt gatctcttgg
    43081 atagtttctt cgtctaattc ccgtcctttt aataatcgat agcgtactaa tacatcttca
    43141 gatacttgta aagcttctcc ttcagataaa tctacgcgat agaaagggcc tttgccttta
    43201 ctgattctcg taacggttaa cataaacatc ctcctcgaac atatattctc tatcatacca
    43261 cagaaaatcc ccgtaatcag gtattatatg ttagaataaa ccacagaaaa aagaaaaagg
    43321 gagatactta tggcagaaac aattgtcaaa actggacaaa cggttccttt gaaaatcaag
    43381 cgtttaggca taaatggtga aggaatcggt tatttcaaac ggttgatcat atttgttcca
    43441 tatgcactac caaaagaaga agtactagtg aaaatcacga aagcaactcc tcgctatgca
    43501 gaaggagaat taataaaagt caaaaaaaca agcaaagatc gcgtagtggc accatgtcct
    43561 gtttactatg aatgcggtgg ctgtcaatta caacacttag cttatcaagc tcaattggat
    43621 tttaaaaagg atctgctttt gcaagcattg gaaaaattca aaccagcagg ctttcgatct
    43681 tatcaacttt tgccgacgat cgggatggat gagccttggc attatagaaa caaagcacaa
    43741 tttcagttac gaaaaaataa gcagactcaa aaaattgaag ctggtttata cgaagcaaac
    43801 tctcatcagt tagtccctat aacagactgc ttggttcaag aacctcgaac acaaaaagta
    43861 atgaatactg ttgttcaact attgaataaa tttgatgcac ccatctatga tgaacggaca
    43921 aacagtggaa tcttccgcac aatcatggtt agaatcggtg ttaaaactgg ggagttacaa
    43981 gttgttttta ttactcgcag taaaaaattc ccgcaaaaaa acgcaatgat ccgtgagatc
    44041 aatgaacgac tgcccgaagt tgtttcaatc atgcaaaatg tccaggcaca aaaaacgtct
    44101 ctcgtgatgg gggatgaaac gatccactta tggggaaaag aagccattga agaacaaatc
    44161 aatgaagtga ctttcgatct ttctcctcgt gccttctttc aactcaatcc tcagcagacc
    44221 gaattgcttt atggcgaagg aatcaaagca ttagatgtcc agcctaatga aacgatcgtg
    44281 gatgcttatt gcggagttgg gacgatcggc ttaagtgtag caaaacaagc caaagaagta
    44341 cggggaatgg ataccgttcc tcaagcaatc gctgatgcaa aaaaaaatgc ggaaagatta
    44401 ggatatcaca acactcgata tgaagtcggg acagcagagc aagttctgcc taaatggttg
    44461 aatgaaggtt ttcagcctga tggtatcatc gtagatccac cacgtacggg tttggataac
    44521 gcattgatcc aatcattatt gaaataccct ccaaaaaaac ttgtttatat ctcctgtaat
    44581 gtttctacac ttgccagaga tctggttaat ttaagtaaaa agttcaagat tgaatattta
    44641 cagtccgtcg acatgtttcc tcagactgca cgttgtgaag ttgtcgtgaa gatgacgaga
    44701 ctcgctgact aaatgaaaga ggttgtgaaa aagtctgccc tgtatcaagt aataagaaca
    44761 gccaaacaaa aatagctctt catgttttat gaatactgta cttgtgaccg aagagttaac
    44821 aggaggacac tatttattta aaaaaagggt tgtgacatag tttttgtcac agcccttttt
    44881 gattattatt ggatattcac gtctatcact tgataaaatg catttgtcgt atcgctgata
    44941 ttccatactg ctaaaatcac attatatcct tttcgatcct gcggaagtgt gatcgtttgg
    45001 ttgactgttt ttggtggaac agttgctttg tcgtctattt ttgtgatcaa ttcaaaatca
    45061 ctaaatttca aaggttgatt aggattccaa gttggtttgg taatgtagta atcccaagta
    45121 cttgtccgat gttgtgctgt taaattccaa gtaatattta atgttcctgg attaaccatg
    45181 tttttatgcc atctgttaga tgtctgttca tctaaaggct caaagccgct gactcctgca
    45241 ctagcaattt ttccatcaat gaatgtattt ttggtagctt cgatactttg cggttcccat
    45301 tgtgctcttc cgacattttg atttaagttt cctgctgctg atgttcctag gtatgcacgg
    45361 cttcctggag aagtaatata accatgcgca aaacctggaa tgctaaaacc gaacaaccct
    45421 aaactaacta acccaataaa caataacgta ctaatctttt tcatgtagaa acctccttca
    45481 tttgataacc acattgtaaa cgctttattg tattaacaca aacattatgc ttttataatt
    45541 ttatgttgaa gtaaaaaagg tgaaaaaagt aagatgcaga ttataacaga agtagacgag
    45601 aaaagtacaa atagaaaaca tgagaatcaa atcatatgat ctttttcaaa aacatcattc
    45661 atataaacaa atgttcaact ttttaataac aacaattatc aatttttttc ttacttcaca
    45721 aactaaatac aggtcttgct gtgagccttc atggtaaact aatattatag aaaggagtta
    45781 gtaaaatgaa gatgataaaa gatattcgtt tttgggtttg tgttatagga attgtcatat
    45841 taggcttttt atcagggttg cttagcggaa atcccggaaa atattactat tctttgcaac
    45901 ttccaccatt tgcaccacct agctggatat ttgggccgat gtggacctta ctgtatatat
    45961 tgatgggtat ttctctttat ttgttactta atcacaacaa caaaaaacag cgaaacaatc
    46021 ttgtcggtct ttttgtgatt cagttcattt ttaattttat atggtctgct ctatttttta
    46081 atttaagaaa tattttcatt gcagcaatcg atattacact tttagttatt ttcttgtcag
    46141 tgcttatgta tcagttgtgg ctgcatcatc gcctggcaat gtggctcatg attccttatt
    46201 atttatgggt actattcgca actctattga attattcaat ttatttttta aactaagcgc
    46261 taaaaagaga cgtcctgtaa tttaacacat agaaaaagtg ccacctaagt tagatttttt
    46321 gtctaattta ggtggcactc tttattaacc aaactatttt tttctagtac ccacttgttg
    46381 ttccatactc attattctgg tcataaccat aaccattttg ttcctgtcca gcgtaaggat
    46441 cataactttg agtatcttga cttggtgtat agtactgact tgtatcttct gtttgctgat
    46501 aataggtatt cggatcttgg tactgatcat atgtgttttc tgaataggaa gaataatcgg
    46561 tgtacccttc actattcgat ccatcaccgt ttgtcgaatc gccatcttcc gaatcatcaa
    46621 agaacatcat tgaagcattc tcatttttca aattaggtaa tgtatcgctt gtttttatat
    46681 ttaattgttt cttcagttca ttttggatcg aaagaagatc attttttcct aaaatttgat
    46741 agtagatatc attgatcatt tgtccttcgc cttgcaactg gtcttgtttg attgtttcaa
    46801 acgccggggt gtagttcgtc gctacatcaa gcatgtcatc ccaatctaag tccgtcgtca
    46861 cattgttctc aactgctttt aaaatttttc tataattgct tacgccatct aaacttaaaa
    46921 ctttattgac gattttcgtc actacttccc gctgacgacg ttgtcgaccg atatcgcctt
    46981 ctggatcttc gtgtcgcatt cgagaatagg ctaaaccttt ttctccattc aatgtttgtt
    47041 cacctactgg tacatggaca ccatccaaag taaagtcaat tttattattc actttgatgc
    47101 caccgaccgc attgatcaaa tcttccatgc catccaaatt gatcgttaca taatgatcaa
    47161 taggaatatc gagcagttgc tcaattgaat ccatggccat ttcgacacca ccaaatgcgt
    47221 aggcatgatt taatttatct accgttccat aacctacgat attcgtataa atatctcggt
    47281 ccaaactgat gatcgttgat tttttttgtt tcggattaac tgttactacc atcatcgtat
    47341 cagaacgtcc ttgttccgtt cttcctaatc caccggtatc taaacctaat agcaaaaccg
    47401 aaaaaggttc tttgtcatca atgcttactc tctcagaacg tttggaggat attcgattag
    47461 agtttttaga aattttgttt actgtttggc tcgcttctcc cataaatctg attccgtatg
    47521 ctgataatcc ggcaatcagc aaaacgaata cccctaacaa ggttaataga actttttgcc
    47581 atcgcttcat aatacacctc tgtttcacat tttctaaaat attgtctcat ataactgttc
    47641 aatcagcaaa ttcacttttt aatcataaga aaactttaaa aaacattttt gtgtaacgat
    47701 tattattttt taaaatttct caaaattctt aactatcata ccataccgtg aaaaagatcg
    47761 aaagaccgaa tacacgcagt tcgcaatcgt tttatctttt tttatgtatt ttttccagaa
    47821 aaagcgaaca acaaggtatt ttccaaataa tcgtgcagta aaatgatcgc acataccttt
    47881 cccatctttt taacagtctt tgcctaccgt tcagattatc attcgatttc acgacaattt
    47941 cttcctatga ctggtccttt tgttaaaccc atcttctatt cgttttattt ccagctcact
    48001 gcatggctct taaaatgact cgaactagtg attgcattac catcatcttt taatggatag
    48061 atatgctcct cttcaaagta aaacgatttt cttttaaagt aatagaattg acatggtaat
    48121 gagtatgtat actagtggtt tcacaaatgg tcttttattc aacatccaaa aactgaaatt
    48181 tctttaaaat ccattactaa cgaagaaaat gttccttgct tgataattag ttaggacatg
    48241 tcaagtgaac aaatgcattt acttagccaa aaaatctaaa aacttcataa atagacaaaa
    48301 agacacgact ctctaagaag tcgtgtcttt attcaacaaa ttattataat ttagtcactt
    48361 cagcagcttg aggtccgcgt gcaccttcaa caatcgtgaa ttctacttct tgaccttctt
    48421 ctaggctctt gaagccgtct ccttgaattg ctgagaaatg aacaaatacg tcatctccgc
    48481 cgtctactgt aataaaccca aatccttttt cattgttgaa ccatttaacc gttccgtgtt
    48541 ccataaatgt atatcctcca aaaaagttct tgcaagtaac cttgcaagaa cattataccc
    48601 aatttagaaa acgctagcaa gctttcgctc ttttattttc caaattattt ttaagactga
    48661 tatgacggag gaaatggtta ttttgctgct tcgaaacgtt tgtttacttc atcccaatta
    48721 ataacattcc agaatgctgc aatataatct ggtcggacat ttttatattt caaataataa
    48781 gcatgttccc aaacgtccaa acctagaatt ggtgtctttc cttccatcaa tggagaatct
    48841 tgatttgcag tagaggtaat agctaatttt ccattttcca ttacaagcca agcccatcca
    48901 gaaccaaatc gtccagcggc tgcttttttg aattcttctt taaaagaaga aaaatcacca
    48961 aaagcttcat taatcgcttc ttttatttct cctgtaggtt cgccaccagc atttggtgcc
    49021 atgatttccc agaaaaatga atggttagca tgtccgccac cattgttacg tacagctgtc
    49081 ttgatatctg ttggaatagc gtccatatca gataataatt cttctattgt tttttcgcct
    49141 aattctgggt atttctcaat tgctgaattt aaattcgtca cataagtatt gtgatgctta
    49201 tcatgatgca gatgcatcgt ttcttcgtca atataaggtt ccaatgcgtc atatgcataa
    49261 ggtagatctg gtaatgtgta agtcataaaa aaattcctcc tcaatgtttt cttaataatg
    49321 tctacattac aaagagtaac aaagagaagg aaattcttca attgttatgc ctagtcgtct
    49381 ttttcacttt gacctacttc ttcttcaaaa acctcagttt ctttaaaaat aaacaacttg
    49441 ctgaaaatat agttcgcaag gataacaata atttgagtga tgattttcgc cacatactca
    49501 ttgctgttca taacattgat caacaatacc attgccccca tgtccaatcc gtaagaaagg
    49561 atacggtaga agaagaactt accgcattca ataagtgcat gtgtaaaatt cggtgatttt
    49621 gaatggaata cccaaagttt gttcgttaca aaagcaaaca atacagaaca aaaccacgaa
    49681 atagaattgg ataaggccat acttaaatca aaatattggc gtgcgagagc aaaactaaca
    49741 aaatttacaa tcgtagccaa accaccaaaa aagagataga ctagtatctc ccaatatcct
    49801 tttgcttcta aatatccttt taactgtcga tactgcttca tgagccaacc taccttatta
    49861 aaaattctta tttatcatac tctatatttc aataactttc atctttattt aaaaaaatta
    49921 attttttttg atcctttgcc tttgtctctg cttggatcgt cacatgtgta ataccatatt
    49981 ttaattttaa taaagaaatt atacgaggat atatcttttc aatctcgctt agtagctgat
    50041 cttcaaaatc aatatgtccg tcgaaaataa ttatcttttc gctatactgc caaatatgga
    50101 catgatgaac atcctttacc ccatcgattg cttcaatttc ttgttccatt ttttcatagt
    50161 cgatagcagg tgcactttgt atcaaaatag cggcagcttt tttgatcacc attatcgctt
    50221 ctatcaatat atatatcgaa atgatcagtg taataactgg atcgatccag tagatatcga
    50281 aaaaacggat caaaattgct ccgataatca cacttaccga agacagtgta tcacttaaca
    50341 aatgcaaata actagatttt atattcaaac tttcatctgc ttcttgtcgt aataataata
    50401 ctgagaacaa attcgcgagc aatccaataa ctgctactgt tagcatcaaa tgactattta
    50461 tcttttctgg tgaagtgaac cgcttgaatg cttcaacggt taaaaacaag gataatatga
    50521 tcaatatgaa tgagttgatg aaagcactca atatttctaa acgctggtag ccatacgtac
    50581 gttttttatt tgcttctttt cgggccattt tttgtgcccc gtaagaaaaa ataattgcca
    50641 atgtatcact aaggttatgg aaagcatctg aaacaagaga caaactccct gacataatcc
    50701 ctccaataaa ttctgataaa gtgatcaccg cattcaaaac cactactcca aatagttttt
    50761 ttgatgatct ctgtggctgt gtaggcacta ttccttcctc cattttattt atattggata
    50821 gtctttagac aagaataaca tttttttgat atttcaccaa tcattcagct gtttccttaa
    50881 agcacttgta tttcacttga tacttcgcta gaattgcaag tgatgacact actagatgag
    50941 gagtacttat gacaacaaaa caattaatta tcagttcctt tatacgattc aaagacctaa
    51001 aggaggcgag aaatgtccca ttttggaaag ccattgtcta tcttttagtt ttgagtataa
    51061 ttatggcttt gccaatctct tttcaagttt ttcaagtctt aaacaacatt caattggatg
    51121 ggcaaaagat tgctactcgc attcctgatt ttacaatcca aaacgggcag cttcaaaccg
    51181 aagaaaaaga aggattcatt taccaaacaa actccattat cttcacattt gatccagagg
    51241 gaaaacgaac agaacaggac atctctactg atttgatggg gaattttgtt agtgtcggga
    51301 tgttaaaaaa caaacttatc atagctcttc ctaatacagg cacgacttct gctttgttga
    51361 acaataacca attagaactt ccctacacca atgaatcttt aaaaaactta acaggtaagc
    51421 agctccgttc ttttttaagt gaagcatcta taccactttg gataaaagtc ctgacattta
    51481 tattttctat ttatcctagt tttctgaatc tgctgatcac tttgctattt accaacattg
    51541 ctgcttttct ctatgcacgc tttcgactaa caaaagctac ttttcttgat tgtttaaaaa
    51601 cattgatcta ttcaatttca ttgcctacta taattgcaac aatcttgatg gtctttcttc
    51661 cttcttttga cactagcgcc ttcattgctt ttgcaggaat ctttattttt gctcaagctg
    51721 tcaaaggatg ggcaaaaata tcgatcaatt aagaaataaa gacaactcgt tatttctact
    51781 tcaatagacc cttttagagg ttgtgacatt tttttgtcac agcccctttt ttatttgaaa
    51841 aatatttctt tctcaaaaaa caagcaaggc gcttcaaagt tcttcttgta tgcgctacac
    51901 ttagagtaaa gaaaggcggg aaattaacta tgtcagaaag aatcatttat gcagatcgtc
    51961 catttcgtga tgaatttatc gaagaaatcc atactatagc gcctggttat atctttaaaa
    52021 cagaattaca accagaggat ctacaaaatg tagagatcag tttgggctgg aactcagact
    52081 accaagaaaa actattagcc tcttctacct tgaaatgggt ccaatctatc tcagctggtg
    52141 ttgatcagct accccttgag actttttcaa agaaacatat tttactttca aatggtagtg
    52201 gcatccacag tgaatcgatt acggagcatg tcttaggaat catccttggg tattctcgtg
    52261 gtctttttca agcacaaaag gcacagagtg acatgaaatg gttaggttca tcggtccatt
    52321 accaatctat ccaagggaaa aaaatactaa tcattggaac tggccatatt ggaaaaatga
    52381 ttgcaaaaaa atgcaaagca ttagacatga actgctatgg aatcaataca tccggtcatc
    52441 cagcagaagg aatggaaaag actttttctt taaatcaact aaaggaagtt cttcctgaag
    52501 tagatttcgt agtcaatatc ttacctctaa cggaggaaac aactgggctc tttgatcagc
    52561 atatgtttga acactttgat ccaaaatctg tattcatcaa tgttggccgt ggtgcttctg
    52621 ttaagacaca agatctggtg caggctttaa ataaccagca actttccttt gcagcattag
    52681 acgtttttga ggaagagcct cttccacaag atcatcctct ttggaaaatg gacaatgtac
    52741 tgattacttc ccacattgca ggtttgacac ctgattttca aaagaaatta atggctattt
    52801 ttttaaccaa tctgaaaagc tactttgcca cccatgagct tcaaaccaat caagtaaaat
    52861 tgtctgcagg atattaatag ctgaaaacaa tattttcaaa agaaaa
//
LOCUS       DACEPD010000012        52139 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.012, whole genome shotgun sequence.
ACCESSION   DACEPD010000012 DACEPD010000000
VERSION     DACEPD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.012"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            330..1175
                     /locus_tag="IW797_000659"
     CDS             330..1175
                     /locus_tag="IW797_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1223454.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="IW797_000660"
     CDS             1456..2676
                     /locus_tag="IW797_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ1223455.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="IW797_000661"
     CDS             2673..5393
                     /locus_tag="IW797_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1223456.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="IW797_000662"
     CDS             5377..6321
                     /locus_tag="IW797_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1223457.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="IW797_000663"
     CDS             complement(6390..7400)
                     /locus_tag="IW797_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1223458.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="IW797_000664"
     CDS             complement(7580..7924)
                     /locus_tag="IW797_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1223459.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="IW797_000665"
     CDS             complement(7917..8351)
                     /locus_tag="IW797_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ1223460.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="IW797_000666"
     CDS             8489..9226
                     /locus_tag="IW797_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1223461.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="IW797_000667"
     CDS             9219..10430
                     /locus_tag="IW797_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1223462.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10637..11416
                     /locus_tag="IW797_000668"
     CDS             10637..11416
                     /locus_tag="IW797_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ1223463.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="IW797_000669"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="IW797_000669"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ1223464.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IW797_000670"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IW797_000670"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ1223465.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="cpaF"
                     /locus_tag="IW797_000671"
     CDS             14132..15061
                     /gene="cpaF"
                     /locus_tag="IW797_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ1223466.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="IW797_000672"
     CDS             15018..16067
                     /locus_tag="IW797_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ1223467.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="IW797_000673"
     CDS             16064..16342
                     /locus_tag="IW797_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ1223468.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="IW797_000674"
     CDS             16317..16787
                     /locus_tag="IW797_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ1223469.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="IW797_000675"
     CDS             16804..17106
                     /locus_tag="IW797_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223470.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="IW797_000676"
     CDS             17123..17494
                     /locus_tag="IW797_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ1223471.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="IW797_000677"
     CDS             17466..17825
                     /locus_tag="IW797_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223472.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="IW797_000678"
     CDS             17997..19004
                     /locus_tag="IW797_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1223473.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="IW797_000679"
     CDS             19029..20213
                     /locus_tag="IW797_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ1223474.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="IW797_000680"
     CDS             complement(20650..22233)
                     /locus_tag="IW797_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1223475.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="IW797_000681"
     CDS             complement(22811..22954)
                     /locus_tag="IW797_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ1223476.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23176..23655)
                     /locus_tag="IW797_000682"
     CDS             complement(23176..23655)
                     /locus_tag="IW797_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1223477.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23728..24012)
                     /locus_tag="IW797_000683"
     CDS             complement(23728..24012)
                     /locus_tag="IW797_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223478.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24090..24284)
                     /gene="ssrS"
                     /locus_tag="IW797_000684"
     ncRNA           complement(24090..24284)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IW797_000684"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24356..25642)
                     /locus_tag="IW797_000685"
     CDS             complement(24356..25642)
                     /locus_tag="IW797_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ1223479.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRCVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25780..26451
                     /locus_tag="IW797_000686"
     CDS             25780..26451
                     /locus_tag="IW797_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ1223480.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26417..26905
                     /locus_tag="IW797_000687"
     CDS             26417..26905
                     /locus_tag="IW797_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ1223481.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26918..27865
                     /gene="prmA"
                     /locus_tag="IW797_000688"
     CDS             26918..27865
                     /gene="prmA"
                     /locus_tag="IW797_000688"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ1223482.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27866..28630
                     /locus_tag="IW797_000689"
     CDS             27866..28630
                     /locus_tag="IW797_000689"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ1223483.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28863..31076
                     /locus_tag="IW797_000690"
     CDS             28863..31076
                     /locus_tag="IW797_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ1223484.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31087..31533
                     /locus_tag="IW797_000691"
     CDS             31087..31533
                     /locus_tag="IW797_000691"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ1223485.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31704..32963
                     /locus_tag="IW797_000692"
     CDS             31704..32963
                     /locus_tag="IW797_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ1223486.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33052..33999)
                     /locus_tag="IW797_000693"
     CDS             complement(33052..33999)
                     /locus_tag="IW797_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1223487.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34393..35685
                     /locus_tag="IW797_000694"
     CDS             34393..35685
                     /locus_tag="IW797_000694"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ1223488.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35700..37469
                     /gene="aspS"
                     /locus_tag="IW797_000695"
     CDS             35700..37469
                     /gene="aspS"
                     /locus_tag="IW797_000695"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ1223489.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37578..38453
                     /locus_tag="IW797_000696"
     CDS             37578..38453
                     /locus_tag="IW797_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1223490.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38719..40602
                     /locus_tag="IW797_000697"
     CDS             38719..40602
                     /locus_tag="IW797_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1223491.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40677..40961)
                     /locus_tag="IW797_000698"
     CDS             complement(40677..40961)
                     /locus_tag="IW797_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ1223492.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40951..41685)
                     /locus_tag="IW797_000699"
     CDS             complement(40951..41685)
                     /locus_tag="IW797_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ1223493.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41814..42437
                     /locus_tag="IW797_000700"
     CDS             41814..42437
                     /locus_tag="IW797_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ1223494.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42629..43750
                     /locus_tag="IW797_000701"
     CDS             42629..43750
                     /locus_tag="IW797_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ1223495.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43757..46885
                     /locus_tag="IW797_000702"
     CDS             43757..46885
                     /locus_tag="IW797_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ1223496.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46904..50098
                     /locus_tag="IW797_000703"
     CDS             46904..50098
                     /locus_tag="IW797_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1223497.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50244..50951)
                     /locus_tag="IW797_000704"
     CDS             complement(50244..50951)
                     /locus_tag="IW797_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1223498.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51716..52114
                     /gene="spxA"
                     /locus_tag="IW797_000705"
     CDS             51716..52114
                     /gene="spxA"
                     /locus_tag="IW797_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ1223499.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        17112 a         9068 c        10788 g        15171 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat
    23041 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
    23101 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
    23161 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
    23221 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
    23281 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
    23341 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
    23401 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
    23461 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
    23521 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
    23581 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
    23641 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
    23701 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
    23761 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
    23821 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc
    23881 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
    23941 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
    24001 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
    24061 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
    24121 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
    24181 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
    24241 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
    24301 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
    24361 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
    24421 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
    24481 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgcaacggt
    24541 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
    24601 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
    24661 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
    24721 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
    24781 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
    24841 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
    24901 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
    24961 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
    25021 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
    25081 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
    25141 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
    25201 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
    25261 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
    25321 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
    25381 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
    25441 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
    25501 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
    25561 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
    25621 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
    25681 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
    25741 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
    25801 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
    25861 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
    25921 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
    25981 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
    26041 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
    26101 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
    26161 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
    26221 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
    26281 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
    26341 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
    26401 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
    26461 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
    26521 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
    26581 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
    26641 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
    26701 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
    26761 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
    26821 ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac
    26881 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
    26941 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
    27001 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
    27061 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
    27121 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
    27181 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
    27241 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
    27301 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
    27361 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
    27421 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
    27481 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
    27541 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
    27601 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
    27661 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
    27721 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
    27781 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
    27841 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
    27901 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
    27961 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
    28021 cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
    28081 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
    28141 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
    28201 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
    28261 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
    28321 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
    28381 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
    28441 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
    28501 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
    28561 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
    28621 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
    28681 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
    28741 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
    28801 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
    28861 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
    28921 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
    28981 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
    29041 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
    29101 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
    29161 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
    29221 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
    29281 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
    29341 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
    29401 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
    29461 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
    29521 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
    29581 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
    29641 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
    29701 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
    29761 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
    29821 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
    29881 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
    29941 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
    30001 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
    30061 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
    30121 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
    30181 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
    30241 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
    30301 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
    30361 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
    30421 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
    30481 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
    30541 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
    30601 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
    30661 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
    30721 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
    30781 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
    30841 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
    30901 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
    30961 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
    31021 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
    31081 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
    31141 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
    31201 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
    31261 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
    31321 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
    31381 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
    31441 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
    31501 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
    31561 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
    31621 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
    31681 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
    31741 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
    31801 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
    31861 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
    31921 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
    31981 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
    32041 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
    32101 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
    32161 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
    32221 gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa
    32281 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
    32341 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
    32401 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
    32461 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
    32521 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
    32581 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
    32641 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
    32701 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
    32761 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
    32821 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
    32881 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    32941 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    33001 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    33061 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    33121 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    33181 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    33241 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    33301 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    33361 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    33421 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    33481 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    33541 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    33601 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    33661 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    33721 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    33781 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    33841 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    33901 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    33961 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    34021 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    34081 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    34141 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    34201 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    34261 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    34321 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    34381 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    34441 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    34501 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    34561 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    34621 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    34681 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    34741 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    34801 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    34861 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    34921 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    34981 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    35041 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    35101 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    35161 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    35221 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    35281 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    35341 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    35401 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    35461 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    35521 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    35581 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    35641 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    35701 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    35761 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    35821 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    35881 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    35941 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    36001 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    36061 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    36121 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    36181 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    36241 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    36301 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    36361 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    36421 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    36481 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    36541 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    36601 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    36661 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    36721 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    36781 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    36841 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    36901 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    36961 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    37021 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    37081 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    37141 gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    37201 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    37261 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    37321 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    37381 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    37441 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    37501 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    37561 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    37621 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    37681 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    37741 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    37801 tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    37861 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    37921 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    37981 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    38041 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    38101 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    38161 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    38221 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    38281 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    38341 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    38401 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    38461 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    38521 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    38581 aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg
    38641 acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat
    38701 gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact
    38761 gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca
    38821 actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg
    38881 atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt
    38941 tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg
    39001 gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc
    39061 agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga
    39121 tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat
    39181 ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc
    39241 ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca
    39301 ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg
    39361 ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa
    39421 caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc
    39481 ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt
    39541 ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg
    39601 ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag
    39661 tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg
    39721 catactttag tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg
    39781 gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc
    39841 agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca
    39901 attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa
    39961 ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa
    40021 aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa
    40081 gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct
    40141 gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa
    40201 ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact
    40261 gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg
    40321 gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg
    40381 caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg
    40441 attgattggt acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat
    40501 ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga
    40561 agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca
    40621 ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat
    40681 atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg
    40741 gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct
    40801 gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca
    40861 ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga
    40921 tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc
    40981 tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc
    41041 taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg
    41101 gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc
    41161 taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact
    41221 tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg
    41281 tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac
    41341 taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac
    41401 agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg
    41461 aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac
    41521 tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg
    41581 agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat
    41641 atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa
    41701 tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc
    41761 aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt
    41821 atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct
    41881 atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta
    41941 cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg
    42001 caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt
    42061 ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga
    42121 aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta
    42181 aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc
    42241 aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg
    42301 aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac
    42361 gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg
    42421 aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc
    42481 tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt
    42541 gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa
    42601 ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg attggcatat
    42661 cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat
    42721 actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga
    42781 taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa
    42841 tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact
    42901 ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga
    42961 agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc
    43021 aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc
    43081 caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag
    43141 ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg
    43201 gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac taggacattt
    43261 gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt
    43321 ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct
    43381 ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt
    43441 caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat
    43501 ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc
    43561 tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa
    43621 agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga
    43681 agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga
    43741 aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat
    43801 caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa
    43861 gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac
    43921 ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga
    43981 aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc
    44041 agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac
    44101 gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc
    44161 agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt
    44221 actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt
    44281 tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa
    44341 agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa
    44401 aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca
    44461 atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac
    44521 ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa
    44581 aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa
    44641 agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt
    44701 cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag
    44761 tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa
    44821 tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa
    44881 caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca
    44941 attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc
    45001 tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca
    45061 agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga
    45121 aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc
    45181 aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca
    45241 gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg
    45301 ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca
    45361 gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt
    45421 agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca
    45481 attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga
    45541 acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac
    45601 aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgcaag
    45661 aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta
    45721 caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag
    45781 tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga tacgacgact
    45841 tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca
    45901 attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga
    45961 aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca
    46021 gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa
    46081 agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct
    46141 gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca
    46201 acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca
    46261 agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag
    46321 ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga
    46381 aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt
    46441 acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc
    46501 ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca
    46561 ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact
    46621 ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga
    46681 tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat
    46741 ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag
    46801 agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag
    46861 caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc
    46921 agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt
    46981 ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt
    47041 tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca
    47101 taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg
    47161 tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat
    47221 cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt
    47281 gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag agctttctat
    47341 tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa
    47401 aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca
    47461 gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca
    47521 tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg
    47581 aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca
    47641 gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca
    47701 attttccgat caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt
    47761 gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca
    47821 agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt
    47881 gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt
    47941 taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga
    48001 agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa
    48061 aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca
    48121 tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt
    48181 acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt
    48241 gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt
    48301 tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca
    48361 accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg
    48421 gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta
    48481 tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca
    48541 agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa
    48601 aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc
    48661 gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa
    48721 aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt
    48781 aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca
    48841 gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt
    48901 agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt
    48961 aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac
    49021 agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca
    49081 aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac
    49141 tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat
    49201 cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc
    49261 gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt
    49321 tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa
    49381 tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat
    49441 gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact
    49501 tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta
    49561 ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa
    49621 caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga
    49681 gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg
    49741 gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa
    49801 ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg
    49861 gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa
    49921 tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact
    49981 ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac
    50041 gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga
    50101 gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt
    50161 tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat
    50221 ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga
    50281 tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg
    50341 ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta
    50401 tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa
    50461 atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga
    50521 atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt
    50581 gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc
    50641 tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct
    50701 tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc
    50761 atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg
    50821 cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg
    50881 accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact
    50941 gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac
    51001 tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt
    51061 tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa
    51121 acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt
    51181 taatgcaatc tctgtggaat tttcgaaaga tttatttttt taatacagtc tcaatataac
    51241 tattcaaaca taaaataacg ttatttgatt tatttgttta tagtaaattt tatttagtaa
    51301 tcattgcttt aaaatactac gcttatataa cagagaaaat aagcgttata ataaagaaca
    51361 gtattattta aaatgacaga aaacattact attaaagaaa taaaaaacat aaaaaatctt
    51421 aagaaatcgg tttcatttag cttttgagat aaaataatag tacagtttta tacgctgttt
    51481 catatgttta gaaaatttga attagatttg aaaactctaa tttttaaatg ataaaaaaag
    51541 cgagaatgct ggttttactg tatattaacc ttagataact ttcatttttt taaaaaaatt
    51601 atgaaaaaaa ggtagctatt taaaagttga ttatgtataa tagaaacaga tgggagatta
    51661 ttatgatctt ctgttacaaa aaagtgattc ttatcacgaa aaggagtggt gcacgttgtt
    51721 aacattatat acttccccaa gttgtacttc ctgcagaaag gctcgtgcgt ggttgcaaga
    51781 acatcagatt ccatttgttg aacgtaatat tttttcagaa ccattgaaca gttctgaatt
    51841 aaaagcgatt ttacaaatga cagaagacgg aacagaagaa atcatctcga cacgttccaa
    51901 agtattccaa aaactgaaca tggatttaga tgatctgccg ttacaagaat tattggaact
    51961 tgtgcaaaac aatcctggat tgcttcgccg tccaatcatg attgatgata aacgtctgca
    52021 agttggtttt aatgaagatg aaattcgtcg gttcctaccg cgcgatgtac gacaattaga
    52081 attacgtcaa gcacaattga tggctggatt atagaaggat aatacgaaac ttggaaaag
//
LOCUS       DACEPD010000013        41205 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.013, whole genome shotgun sequence.
ACCESSION   DACEPD010000013 DACEPD010000000
VERSION     DACEPD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.013"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..328)
                     /locus_tag="IW797_000706"
     CDS             complement(26..328)
                     /locus_tag="IW797_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1223500.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(353..2446)
                     /locus_tag="IW797_000707"
     CDS             complement(353..2446)
                     /locus_tag="IW797_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1223501.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(2935..3699)
                     /locus_tag="IW797_000708"
     CDS             complement(2935..3699)
                     /locus_tag="IW797_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ1223502.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IW797_000709"
     CDS             complement(3810..4679)
                     /gene="mdcB"
                     /locus_tag="IW797_000709"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ1223503.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(4704..5780)
                     /locus_tag="IW797_000710"
     CDS             complement(4704..5780)
                     /locus_tag="IW797_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ1223504.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(5804..6397)
                     /locus_tag="IW797_000711"
     CDS             complement(5804..6397)
                     /locus_tag="IW797_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ1223505.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IW797_000712"
     CDS             complement(6401..8077)
                     /gene="mdcD"
                     /locus_tag="IW797_000712"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ1223506.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(8067..8366)
                     /locus_tag="IW797_000713"
     CDS             complement(8067..8366)
                     /locus_tag="IW797_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ1223507.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IW797_000714"
     CDS             complement(8468..10114)
                     /gene="mdcA"
                     /locus_tag="IW797_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ1223508.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(10121..11032)
                     /locus_tag="IW797_000715"
     CDS             complement(10121..11032)
                     /locus_tag="IW797_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ1223509.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            11152..12009
                     /locus_tag="IW797_000716"
     CDS             11152..12009
                     /locus_tag="IW797_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1223510.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(12196..12558)
                     /locus_tag="IW797_000717"
     CDS             complement(12196..12558)
                     /locus_tag="IW797_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223511.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            12904..14343
                     /locus_tag="IW797_000718"
     CDS             12904..14343
                     /locus_tag="IW797_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1223512.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(14350..16902)
                     /locus_tag="IW797_000719"
     CDS             complement(14350..16902)
                     /locus_tag="IW797_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1223513.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(17016..18347)
                     /locus_tag="IW797_000720"
     CDS             complement(17016..18347)
                     /locus_tag="IW797_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1223514.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(18313..19791)
                     /locus_tag="IW797_000721"
     CDS             complement(18313..19791)
                     /locus_tag="IW797_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1223515.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IW797_000722"
     CDS             complement(20096..21931)
                     /gene="tdc"
                     /locus_tag="IW797_000722"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1223516.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            22401..23828
                     /locus_tag="IW797_000723"
     CDS             22401..23828
                     /locus_tag="IW797_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1223517.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(23920..24285)
                     /locus_tag="IW797_000724"
     CDS             complement(23920..24285)
                     /locus_tag="IW797_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1223518.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(24282..24647)
                     /locus_tag="IW797_000725"
     CDS             complement(24282..24647)
                     /locus_tag="IW797_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ1223519.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(24839..25087)
                     /locus_tag="IW797_000726"
     CDS             complement(24839..25087)
                     /locus_tag="IW797_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223520.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            25086..25214
                     /locus_tag="IW797_000727"
     CDS             25086..25214
                     /locus_tag="IW797_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ1223521.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            25271..26965
                     /locus_tag="IW797_000728"
     CDS             25271..26965
                     /locus_tag="IW797_000728"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ1223522.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            26969..27088
                     /locus_tag="IW797_000729"
                     /pseudo
     CDS             26969..27088
                     /locus_tag="IW797_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            27170..27361
                     /locus_tag="IW797_000730"
     CDS             27170..27361
                     /locus_tag="IW797_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223523.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            27739..28872
                     /locus_tag="IW797_000731"
     CDS             27739..28872
                     /locus_tag="IW797_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ1223524.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            28903..29223
                     /locus_tag="IW797_000732"
     CDS             28903..29223
                     /locus_tag="IW797_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223525.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(29252..29716)
                     /locus_tag="IW797_000733"
     CDS             complement(29252..29716)
                     /locus_tag="IW797_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1223526.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(29847..31100)
                     /locus_tag="IW797_000734"
     CDS             complement(29847..31100)
                     /locus_tag="IW797_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1223527.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(31129..31446)
                     /locus_tag="IW797_000735"
     CDS             complement(31129..31446)
                     /locus_tag="IW797_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1223528.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(31472..31792)
                     /locus_tag="IW797_000736"
     CDS             complement(31472..31792)
                     /locus_tag="IW797_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1223529.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(32256..33671)
                     /locus_tag="IW797_000737"
     CDS             complement(32256..33671)
                     /locus_tag="IW797_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1223530.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(33688..35004)
                     /locus_tag="IW797_000738"
     CDS             complement(33688..35004)
                     /locus_tag="IW797_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1223531.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(35154..35852)
                     /locus_tag="IW797_000739"
     CDS             complement(35154..35852)
                     /locus_tag="IW797_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ1223532.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(36141..37130)
                     /locus_tag="IW797_000740"
     CDS             complement(36141..37130)
                     /locus_tag="IW797_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ1223533.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(37210..38043)
                     /gene="msrA"
                     /locus_tag="IW797_000741"
     CDS             complement(37210..38043)
                     /gene="msrA"
                     /locus_tag="IW797_000741"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1223534.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(38218..39852)
                     /locus_tag="IW797_000742"
     CDS             complement(38218..39852)
                     /locus_tag="IW797_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223535.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(39839..41173)
                     /locus_tag="IW797_000743"
     CDS             complement(39839..41173)
                     /locus_tag="IW797_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223536.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        12502 a         8474 c         6958 g        13271 t
ORIGIN      
        1 tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga actggggtga
       61 ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc gccgttttag
      121 gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg atcacatctg
      181 gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct actcctaatt
      241 tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc
      301 atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt tattatttct
      361 caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca atcaatcgat
      421 ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc agatgcgagt
      481 aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca ggattaccga
      541 aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc ccatcttctg
      601 gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg taatcattca
      661 ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta accaatggtc
      721 tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc atgatattgc
      781 tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt tctaaattgt
      841 ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg ccaccacttt
      901 ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg ttaggctcta
      961 accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata ttcgtctcag
     1021 ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga aaggtgctga
     1081 aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt aaaactgcag
     1141 taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt ccagtagcca
     1201 tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc ttgattacac
     1261 tggcgatcgt cgaaaagtgg atgacaagaa aagctatctc gtcctccagt atcttgccac
     1321 caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt gggaaataat
     1381 ttgttcggat gttttctttc aatggattca tgacttggat atgattcttg attcttacaa
     1441 tcagtgcagc catgtgctta tagagatttt cgaataaagc ttcttccttt agatggaaag
     1501 gtacgtgtgt ctcgttctct acaaattgga tcaattggat cgttagtaat tgagcttgta
     1561 cccactcgat tgaattggag atatctctag cattgaacgt ctcaatagtg aaacgaagat
     1621 aatttctttc tacttgcggg acattgtcca attgaaattc ctcgatcatg tggtcaataa
     1681 atgcagaaaa gctactgtct tttatttctg tatttttcca attaatggca gagataaatt
     1741 catgtttcct gattcgctgg atccagatca atgtaaaaag caggatttgt tttttataaa
     1801 tatcatcttc ttgtttcaac aacttattgg aatagatctc ctgtattttt ttgatttcca
     1861 ccaaaggaaa ataatggaga aagatttttt gagccggtgt taattgtgca tcttgattca
     1921 taaagaagtc ggtcattccc agattctgat tgataaaatc atagatcatc gtacggatag
     1981 atcgttctga acctttataa accagtcctt gctttccata actgacgatc tctatcccat
     2041 aactgaccag tgttgctcgt aaccgtctca tatcttcatc taatgtactt ttagagattt
     2101 gaaattcttc ttcttttttg tttagatgga tcgggattcg gctgtaagca acggctaaaa
     2161 ctagatcaaa aatacgttcg cgccgattca aaaagccgtc tttcacatct tttatttcag
     2221 tttctaatcg agcttgcttt tccttttcca gcttcaacaa aaaacctttg ttgcgaattg
     2281 tacggatctc cggaagcttt ttactgctca accagatatt cgcttcaaga acttcctgac
     2341 ggatcgtccg ctgactgaca ttgaactcac ttgctagatg ttttgctttt actggatatt
     2401 gggctgtcat gattctgtga agtatttcaa ttgttcttgt tttcatagaa atgatctcct
     2461 tctgatttca agtatatggt tccgcttaca taataaaaag tagatatttc tgccacttga
     2521 agtggaaaga aagtaagcaa agcaaacaaa aaataagcca tatgcttttt catctcctca
     2581 acgaaaaaga catacgccta ttattacaaa aattttgccc atctatacaa aaaagaggtt
     2641 gtgaaaaagc tgtcctgcat caagtaataa gaacaactaa actaaaatag tgcttcctat
     2701 ttttcgtttg gttgttctta ttatcgcaga tgcaagcttt taaacaccat ttagtggaga
     2761 ggttgtgaca gagatgttca gccttgagcg ataagaagcg attttgaaaa atcgcttccc
     2821 atattttaca aaatcgtgcc ttattgccga taggctatct ctggcacacc gtttattcag
     2881 aaaaaggagc tgcgacaagg cttttgtcac aactccgttc ataaatattt cctgtcaatc
     2941 cagcataatc ggctgaataa aatcggcttg ccatcttttt tctttctcag aaaaatcaaa
     3001 ataccatatc tgagaaaaat actgtcgttg tgtcttactt cctgagatca atcgcccatc
     3061 ttttcgatcg actgaataat cacagcatct gaaatcaatt cggatggcaa aactattttc
     3121 gtggatctgt cgataatttc ctacttcctg aatagttatt tttttagtat gatttcttac
     3181 accgtctgct ttgttttttt gcagaacttt ctgatgttcg gcaaacagct tgtctgtata
     3241 acagcttctg gctttttcag gagtttcttc ttcccaagcg gattggatct tcatgaatat
     3301 cttttggatt tccagaaact gtgctttctt ttctttagcc gttcctgaca tgcgggacaa
     3361 tatctccgct ttcagccttt tcttttgtct tttattcttg acgatagaaa aagcagaaac
     3421 ccctcctact gacaaaacag ctaatgcaag aaatcccgtt tgctcataac tagaaggacc
     3481 tgtacctgga ccataatacc cataagaatc tctgccgtaa gaacgactgc caaaataact
     3541 gcccccatat gaacgaccac caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc
     3601 tccaccatga ccacccgcga cagcgaatcc ttgggtcggc agagcaaaca aacataggcc
     3661 cactaacaaa atgattgcta aataattgat ttttttcatt gtcttcctcg ttatctttta
     3721 ttacaaaaaa ctatttttat gatagccttg ctaaaaggaa gagtcaataa ccagatgcat
     3781 aaactgtttt tttagcaatt ttgtcttatt tagtcattga gtaacatcgg acaaatgtat
     3841 gtgtcggcag tccataattc ctctatcttc agcatgaaca agcttgcaga caagagatct
     3901 gcgcttcctc caggagagat ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga
     3961 cgatttggta aagcactttg actagcagca tgagcaagtt tttgcatttc cactaacgtt
     4021 ttctgatcac tgcgatgcaa tacgcatgtg tcatctagcg aactcatcaa aactaacaac
     4081 atatgcattt gtttctgtac atatgaatca ttatctagct ccttagctgc ttgaattgca
     4141 agcctgatat gcggataccc taatatcgct tcttcatatg caccatttac gccatatttt
     4201 tgcttcaccc tatgaccatg agtggttttt ttatgacgat attttgtatc aggaaaagca
     4261 gcaagcttgc tgacatcatc aagtattttc gataaagaaa aaggctgttc catactgatt
     4321 ttttgtgcaa tgacactgat caatagcccc attgcccaaa tcgctccttt atgggtattg
     4381 atgccatttg tagcaatcag cattgccttt tccgcttttc gcccaatctc agcaattctt
     4441 tctcttaact cctgatcaat agcatgatgc catgctattt gagccatttc ttcaaaaaaa
     4501 ggagttagcg tcttagcaga cttaagaaat aaagcatagt ccatatcgga atgtgcaccc
     4561 gcatccagtg catccactaa tccaggtttt ggacttaatg ttacttctgc gatcaatgct
     4621 tcctctacca acgcagcgag ttgctgagtc actgcttttt ttcgttcgtc atttatcatg
     4681 acactcactc cttcttccta ctattacttg ctgaaacctt ttttgatagc tacccaaatg
     4741 atatgaagta accagcttaa agcaaatgca caacttgccg tcattagata agctacaaga
     4801 atcatgactc ctgaaggtaa ctgcagccaa ctatctttaa acaataataa cagtacttgg
     4861 aaaacaaata cattggctaa atacgtacga taagcataaa cagacaacca tttgaacaat
     4921 ggcaattttg gcgaatcgtt tacaatcatt ttggaagcgc ctatgaagac agcaaagatg
     4981 atgactaaac tgtataaact ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag
     5041 tctcctggat gtgcgagcaa tgaacggaca gaaaagaacg cgataactaa ggcaacaggt
     5101 aagaaaagat aacgtactct gtccaaatat tgatagactt tttcgtgata aacaaatgcg
     5161 gctaccccat aaatcatgaa actagcaaca aaccgatcta ataagtacca gctgtcatga
     5221 taaggcccag tcaatacttg tgtttgatag aacagatacc atgcgacata taaaatcgtt
     5281 gaaccaatca atactggcca tacatatttt ttattagcaa atactttgta accgagccaa
     5341 acaaaaaacg gcatgaacaa ttggatctgc agcatcataa cggtatacca taaatgaggc
     5401 gctccatcac ctgtgacata tttcagtaaa aagcttgcag tatccgtata cggagtttgt
     5461 tgttggactg ctggaaatag ccaaagataa gccgtcgtcc atagaagata cggaacgact
     5521 agttcaaaaa acttttcttt caaaaattcc caataagaca aatgctgact tgtttttacc
     5581 atattataaa caattgcaaa aataaacatt ggtgctgtgt atttaacgat gacgtaaaag
     5641 gtaccaagaa aagctgtatt tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc
     5701 gtttgcatca taacagccgt tacagctgat actttctgga agtcacttac gttgattttg
     5761 atttttttgt tttcaaacat ctctttctct ctctttttct ttattaccaa agttctttgg
     5821 ttaatttagg tccttcgact gttttcaata gaattttttt ctctggctct ctcaaatact
     5881 cttttagtga aaaaccgcct tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag
     5941 caggatgcga ttgaattacc tgaattggca attcaatcgg tgaatttgcg tacagcagca
     6001 aatcaaaatc actggtttct tttactgccg gaatcccagt cgataattca aatcctaagc
     6061 tgccgccgat tccccattta catcctgata atatgtttct agcagcatga aattgctgat
     6121 aaacaggata atccatgatt ttctcttgtc tatacgattc ttgttcgatg atgtcccacg
     6181 gatgaatgac ttgtttgacg gcatgttttg agatttctgt tgaccatctc tgattgcggg
     6241 caggtccacg taaaccaatc ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg
     6301 ttccgaacat cttttcagtt agaacgaccc attcaggcaa ggtgaaccct tctatttcat
     6361 gtttatcaaa aaggacaata tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt
     6421 ccattttggc acggacttta tttgtttcga cacgtccgaa tgttttggct gtttcgtttg
     6481 tgtatcgatt tgtcagcatc gtgtcatttg tattgcggac atcttgaatg gcttcattga
     6541 ttgcttcgat gactttgctg acattttgtt ctgaagcagc ttggtcggtg atatcttcta
     6601 ggaaatgata gatcgctcct agttttgtat aattattgat atcgtaagcg atagctggaa
     6661 ctttttcagc agctgcttcg atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt
     6721 ctttagacat cgcttggacg gtaatttctt tattgtttaa agcaatcaag cggcttgatt
     6781 gtaatccatg tgctaaaaat gcacctgaaa tagcatttcc tgcaatcaat ccaataactg
     6841 gatgtccagc ttgacggagt tttgcataag cagcagcact attagccaaa gccacatgga
     6901 taccgatcaa ttcttcgtta tagccgtatg cttggcttgg tacatcgatc acaacgatga
     6961 ttggccgttt cacttttttg tctttatctt cttcgtaaac gtgatccaaa atttgtccca
     7021 tgcggaaacc ttcaactaaa ccaacttcac cgtttcgtac acggtacatc gggtttttag
     7081 gatcaggtac gattgatgtc acgatggctt cttctatgaa tttacttgat ttaccataat
     7141 acacagtact aatagaagaa gttggatttt tcatgccagt tagtgccgca aaccaatcgt
     7201 aaccaacact tgctgcagct ggttcatttc cttctgctac ttctggtaag tcaattgatt
     7261 ttgcttgatg gttagcaatt gcttcattgt aatcgtcaat ggccattggt tttgaaagat
     7321 ctaaagaatc aagtaatgat aaataaaatg ctgagctttc actgcgatgc gtgtcttttc
     7381 gttcgtccat tgctgcgatg acagcagctt tgattgcttc tacactatca gtcactaatt
     7441 catcaatgat ttttgattct acccgttgac gagatcctaa tgtattccaa atcaagtctt
     7501 tgtcactaga atcaaattct cgaacgcctg cttcttgttc aataacttct ggtccattca
     7561 aaccgactct cgcttttttt gttgcgatta aataactcat taatgctgaa gtaatcgaca
     7621 taccgccaaa tgatcctaca cgtcctggaa tcaaaccgat gactggtaca tattgtttta
     7681 gagcaatgat tacatttccg atttctgaaa tagataacag tccgtagttt gcttcttgca
     7741 aacgaacacc acctgtatct agtacaatga ctggaaagat ctcatttcct tttttatttt
     7801 cttctaacac atgttcaagt gcagcaacga tttttgcacc ggaaacttca ccgataccgc
     7861 caccttggaa ttttccttcc atagcgatta ctactgcttg tttgccattg atcgtgccac
     7921 ggctgacaac gatcccgtca tcactttcag ggacgattcc ttgtgctggt aaacttggcg
     7981 cgatgatttg atcgaatgga tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc
     8041 gttctctggc gtttaattct acaaaactat ttttcatctg aaagttcctc cattgcttgc
     8101 gtcaatcgta ggtacacaac tcctggtgta gcaccgaagt cattgatcac gatatctgct
     8161 gaaattggat aacgatcaaa aaatctcgtt aatacattgt tccatacttc gccgaagcca
     8221 tcgcttcctg tacaaatttt tacagtagat tctgtttttt ctgttggaaa catcaagatt
     8281 tccaaatctc ctgaaccgac cactcctaca tggattttct tattgatcgg ctgtttcgtt
     8341 ggatatttat aagctaattg ttccatggat ctctcccctt tttgtaaaac agagcataca
     8401 cgttctgctt attcgtcttt cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc
     8461 gccaatttta tgaccaagtt ctgaatttca ttggtggttc atacaggcca tctgaccatt
     8521 cgatcaaatc atcgatggtt tgtgcagcta ataatgaacg tttcgcttca ctacgtttga
     8581 tgcctagatc ttctggtaat ttcacgatgc cggctgcacg caagctatct agtgattctt
     8641 tcgtgctagt cattccaaga ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt
     8701 cttcttttgt ttttgctttg tacaaataag caattccttc ttctgtcacg atatgtgtag
     8761 tatcttcgct ataaatcatc actggtgcat tggctaagcc tgcttgtttt tgaacagcta
     8821 gtgcatctaa ttcttcgaca aagactggtt ttttatttga accatatgtt tcaaccattt
     8881 gaacaactaa tttttggcct ttccctaatg gatcatcatt gtcgcgaagt gattgccatg
     8941 ctggtgtaga atgacgacga ccgccaggat tatgtcccat gttaggcgca ccgccaaaac
     9001 cagataaacg tccttttgtt acggtagaag agtttccttc gtaatctaat tgcaaggttg
     9061 aaccgataaa catatcgatg gcatattgtc ctgcagcttg tgataatgtt cggttactgc
     9121 gcatcgtgcc gtctttaccg acaaaaaaga catctgggcg agcagcaata tatttttcca
     9181 taccaacttc gccaccaaaa ctatggatgc tttcaaccca acctgattcg atggctggaa
     9241 tcatcgttgg atgtgggttc aatacccagt tcttcgcgat tttccctttt aaccctagtg
     9301 attcaccata agttggcagt aacaattcga tagctgctgt attgaatcca ataccgtgat
     9361 tcaaagattg tacgccgtgt tctgcgtaga ttcctttgat tgccatcatt gccattagaa
     9421 tttgcaattc tgtgatgttt tgaggatctc gtgtgaataa agcttctaat tgataaggtt
     9481 tatctgcttt cacgacaacg tctacccagt caccagggat atctacacgt ggtaaagaat
     9541 cgacaacttc gttaacttga acgatcacga taccatcttt gaaagctgca gcttcgatga
     9601 ttgttggtgt ttcttccgta ttgaaacctg tgtacaagtt gccgtcttta tctgctttat
     9661 ctgcagctac taaaacaaca tttggaatca aatcgatgaa taagcgtcca taaagttcca
     9721 aataagtatg gatatcacct aatttcattg tgccgtcttc aatcatttgg gcgacacgca
     9781 agctttgtgc accagcataa gagaaatcga tcttgcttgc gattcctttt tcaaaaatat
     9841 tcaaatgttc tggtcgtgag atactagaca tgatcaaatg cagatcattc actttttctg
     9901 catctacttt ttcaagactt tctgaaagaa aacttgcttg tttttgattg tcgccttcta
     9961 atacaacttt gtcgccggga gcaatcagtt tttccattac ttctacgatt ttttcagttt
    10021 ctacatactt gccattgagc aagttgctga ttttttccaa tcgctgcgct tttttttcgc
    10081 gtttagaagc ccagttgcgg tttggttttt ccataattag tcatctccat ttttgatata
    10141 gaaacgtggc gtcatcaata ccatgttggt ctagattgat cgtacggatc cctttatcta
    10201 caccaaattt tgtatgaatc agctttgtca aagtattgcc ggggggaaat tcaatcgtca
    10261 gatccattcc taattctttt gccaagtcca tcatttggtt ccactgcaca gggaaagcta
    10321 ggttttccaa caaatcttta cgaatctctt ctgcgtcctg agtggctctt ccatccgtat
    10381 tttttatata caaacatcta gcatcagata gatggacaga ctgcatatac ggcttgaatt
    10441 tcttgaccgt atcattcatc aagagacagt gagatggcac agggactttc agcagcttcg
    10501 ctgtttgcgc ttggtgccgt ttggcttctg ccaacgtaac ggataaacct tcgatactcc
    10561 cagataacgt atgttgcatc ggacaatttt catttgacag ataaactgga tggtcaggat
    10621 caaatgtttt tgctacaatt tcctcagctt cgcttctcgt cacacctact actacgccca
    10681 ttgcataacc gcttggataa gcttctttca ttaagactgc gcggtgatag actagttccg
    10741 ctgcattctc aaatctcaaa gatcgacaag caacagctgc acaaaaagca ccaatcgaat
    10801 gtcctgctgc aatatcaggc tgaataccgt ttttttccat ttcatctaaa taaaatactg
    10861 cttttgtcag taatgctagc tggataaaaa ccgtatcttg atagttttct tctttatcaa
    10921 ctaaccgata ccccgtcaca ttttctaaac gttccatgta catttcaggg atatccgcta
    10981 acatccctaa cttctgactg ccttgtccag gaaataagaa acctgtactc atgagcctcg
    11041 cctcctcagc acttactttt tccactgcta tcttagtcaa tttctgctat aataaaaaat
    11101 gccaaaaaaa gaaaaaacca taacaaaaag gtatggatgg gatggataat tatgaaattg
    11161 cttcatctgc agtattttaa aaaagtattc gaaacaaaaa atgttaccca agcagccaaa
    11221 gaattattta tttctcaacc tgccttgagc cgtgcgatca aacatttgga aaacgaatta
    11281 ggtgtccctt tgttttacca tagcggcaga aacattgaga cgaccgttta tgcagaagaa
    11341 ttttatccat atgcagtcaa gacattagac acattagaag aaggaatcaa attattgtct
    11401 tcggtcaatg agaagatcgt tacttccgtc atattgtatt tagaggtagc ctctgtttct
    11461 attccgaact tggtccgaat ttttatggaa aagcatcctg atatccaatt aacgatcatg
    11521 caacatcatt taccagatga tattaccgat cctgtactgt acatcacttc ggagatgaag
    11581 cctggcatga ccaatattcc tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa
    11641 catcctcttg ctaaaaaaga acatctgcaa ttagaagata tccaacgcac ccccatcttg
    11701 atgctgtcaa agagcaacgc cttccgtcat acactggatg cagcacttga agcgaaaagt
    11761 atcgaattga cgatcagttc gacgacagat gatcctgcga cattacgttc tattgtcaag
    11821 caaggattag ctgttagctt tttcccaaaa gtttcttggt cttacgataa aaatgatcct
    11881 ttcgtcttgc gggaaatcaa agatttgccc ttgacacgaa caatttattt atcttctagc
    11941 ttcacagaag atcatccttt gatcaaaatg attgcaaaaa cgatgcggga attttattta
    12001 ggaaaataaa aaaaaaagag ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa
    12061 cggtgttcca gaagtaattt cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc
    12121 aattttcgta aattccttct tatttcttga agctaaacat ttctgtcaca acctcttctc
    12181 tattgtccta gacattcatt gcgttgattt aaaacttcca tcaatcaaat ctgctagttc
    12241 atctagcttt tctattttac gtagatcaat agagatccaa tgttctttat tcatgtgata
    12301 ggctggaaaa tacccctctt ttcctcgcaa aattccgcca agttcaggat cgaccttcaa
    12361 atcaagaata ttgatgatcc tatggtcact tgcatcatca tagatttttt cttctggaac
    12421 agacatcagg accccaaacc attttttgct tttttgatgt ctgaatacgc aataatcagg
    12481 aaatttttta aaaatatatt ctggagacaa attgtatgtt tcattgatgt gatcgattag
    12541 ttcttttttc gttagcatct gcttccatct ctttcttatt gattcgcttt attcaaagat
    12601 accacgtaag aatagaaaaa tgaatacaag tagttgctaa tcaaatactt cgatattttt
    12661 cttttttatc aactcttaaa tgttcaatcc gcctgtgttg gtcttgctac catctactat
    12721 taaaatgttt attattgttt gcccacctat tttttgattt tagtggtatc atttttcaag
    12781 ttcatgatga aattcttatc atcagtaaat gctccagaaa aataaacaat gattgaaaaa
    12841 tcagttgaaa cttgaccacc gtttattttc tccgtattct tagaatagaa gggagtctga
    12901 ttgatgtatt cactaatgaa aaaaatcatt accgaaaaag atattcgacg tcatgtttct
    12961 ttagtagaac aattgctgaa tcatacaaag ataaccgtaa atgaattagc tgaaatcata
    13021 gggacaaccg aacgaacgat tttttctgac cttcaatcta ttcgctctca gctgcccgaa
    13081 ggctgggaca tcatcagcga tcaagcagga atcagtttgc aaaaccagca aaatttatta
    13141 acaaatgacc tttgggagat ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat
    13201 ctgcttttta caaaaaaagt tgctgtccct gattttctag ccgaccatgg gctttcttat
    13261 gggacattga aaagacatgt gactaaaatc aatcagcgtt tagcaagtta cgacttgcag
    13321 atcgatttga cgaaatatac agcttgtatt cttggaaaag agcgtgcgat tcgtactttt
    13381 tatcatcgat tgctgattcc tttcactcat aacaactatt ttttcgagga ttattcgatc
    13441 catgaatccc attattttca atttttaaga aacctcagtc aaactgaact agcggttgaa
    13501 acagaagaga ttttcggaac atgttggttc tttatcaata cgatccgtat aaaagcgaat
    13561 tgccgattag attcttctat ccataccaac agcacactct cctccctgta tgactctgca
    13621 ttgaaaaaat tgtatttaac ggaaggtatt tatcttaaag acactgagct ttcttttgct
    13681 tctttctgct ttctggaaag ttggaattac aacaataact atggacaaga aaccgctcgc
    13741 tgcctgcatc attctccatt tttagaggtc ctcgaaacgt ttgtagagga actagcttca
    13801 gagctgtctc ttgatcaact aaaaaaaaca tctctgactg ataacttggc tctcctcata
    13861 ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata ccatcgtttt
    13921 ttagaaacat atgatcagca atatgcacac ttgtatcaac agaaagagaa tttactgaac
    13981 gatctaaaaa aagaaataga aatcaatgaa ccaaattact ttctccaact cttgtctcta
    14041 ctgatccaac aaacgatttt ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc
    14101 caaggagaac cttcatggaa ggcgttcttg cagcaggaat taaaagatta cttaggaaaa
    14161 cgtgtctgct tcttacctat cgaactaacc gatctttcca ctatttcttt tgaaaaaaca
    14221 gatatcttga tttccaattt ccctctagat tctctagata tccctattgt atacatttct
    14281 tctattccaa cgaaaaatga gctcaatcgt ttgactgaac tcactttcaa aactttttta
    14341 tagtaacggc tagcgactgt attctctagc tgttttttct tttttgcgaa acagtttgtt
    14401 catgatccct ttttctattt ctacaactaa gaatagaaga atcacatttg ctccaatcaa
    14461 taactgttgt gtcatcgtta aacttactgt accgatcagc tgctggccga ctggtagaaa
    14521 aataaccatc ccttgcaata aagcaaggat acctaatgaa agaaaaagcg ctttgttttg
    14581 caaaagacct tggttcaatg acggcttgga caactctctg caattgatca tgtaaacagc
    14641 ttgagctaag acgatatttt gcagaagaat ggtttgctgt aagctttgcc cttcaaactg
    14701 catagccaat aaataggcag gaaccatgat cagtagggag acatagatga tccgaaacaa
    14761 atgataacca gataggattc cttgctttgg atctcttggt gggcgcttca tgatttctgg
    14821 atctggcgac tcgaacccta gtgcataaga taaagtgatt gtcgttacca tattgaccca
    14881 taaaatctgt actggggaca aaggtagcgg gcgattcagc ataagtgccc aaatcaagat
    14941 caatccttga gctaatgcag ttggcaagaa gaaattgatg gttttttgca aattatccaa
    15001 aatccgccgg ccctctttta cggcttttgc aatcgtatgg aaattatcat ctcctaaaac
    15061 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcaa ttcctacatc
    15121 ggatcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    15181 atttttctgc aaagctttaa caattcgcag tttatgttct ggtgtcgttc gagcaaagac
    15241 atcgactatt ggcacttgct gaatcaactc ttcatcagac ataagatcca aatcgatccc
    15301 ttctaaaact ttggcagtat gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc
    15361 tttgtgatcc cctgtaatca ttttcactga gatccctgct tgttggcatt cctttacagc
    15421 tttgactgca ctttcttttg gcggatcgat caatccggcg atccctgcaa aagttaattg
    15481 ggttaaagtc tcatgcgtga gttcctgttt cgaatctact tttttataag caaatcctaa
    15541 aacacgctga ccttttctcg caaattctgc agctgtctga ctccatgctt cttgttctgc
    15601 agaagaaaga gttgatttag caaataatac ttctggtgca cctttgacgt aaatcaccgc
    15661 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagaactga acggaatctt
    15721 tgcttctatt tttttcaaag gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc
    15781 tgtcggattg ccacttaaat tttctgtttt ctgttcttct tttgttttta attcctgaca
    15841 gtttttcata atggacaaca cgcaggcttc ttcttttgca gctacatcca caactgtcat
    15901 ttcatttttt gtcaatgttc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    15961 ttctaccgaa ggaagtcctt tgatgatcgc cttttctttt gccatttcat gtactccaac
    16021 ggatagaatc atggtcaaaa cagctggcaa tccttcgggt accattgaca ccaccaaagc
    16081 aatcgtagcc gaaagaagga tatgccaatc cataccatat cgaaatgtcg taaaaataac
    16141 taagaatagg ctcaaacaaa gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt
    16201 tttaatcaga ggcgtcgttt gttgttcgac cgattgcaat gcttgattga ttttaccgat
    16261 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    16321 tcctgaaaaa cccatatttt tctggtcgcc gatcgattct tcttctgtca aaacggcatg
    16381 acttttttca gatgcttctg attctcccgt caaaatcgat tcttccacta ataaatcatg
    16441 tgcttcgatt aatcggatat ctgctggaac gacatctccg gcacgtaaga caatgacatc
    16501 tccttttacc agttctttcg cagggatttt ttcttgcatc ccgtttgata gtactatcgc
    16561 ttcttgtccc atcagctgct tcaaaccatt caatgattct tccgcttttc tttcttgcca
    16621 gtaactaacg agtccattga cgatcactac gaataaaata atacttcctt caatatattc
    16681 actagaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    16741 atgctttgct atttttttcc atcgttttgt ctgctgcctt gcttttattt cattatatcc
    16801 ttgatcagtc aggcgtttct gtcgttcttc ttgcgacaag ccttctgttg atgtttgata
    16861 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    16921 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    16981 tctgaaataa ttcgttttgc aaaatgtatc tgggcctaac tgatatttct attgcttatt
    17041 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    17101 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    17161 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    17221 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    17281 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cggcaaaaaa taacattcct
    17341 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    17401 aaactttttt cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    17461 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gccttggcaa
    17521 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    17581 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agagaatacg ccaaggcaga
    17641 cagttgattg cttttttcaa attcttttgc ggatcacttg tctcgccaac ggttaagccg
    17701 atcatttcaa ttccagcaaa actgaataaa accataggaa atgagactac cattccagca
    17761 aagccaaacg gtagtaattc atcgatctgt ccaaaatttt ctttgattcc gatagactgg
    17821 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    17881 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    17941 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    18001 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    18061 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    18121 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatggc ccgcatcaaa
    18181 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    18241 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    18301 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    18361 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    18421 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    18481 tgttccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    18541 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    18601 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    18661 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    18721 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    18781 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    18841 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    18901 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    18961 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    19021 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    19081 tactccgcga atatacgttt taactggatt ttccaatcga gtgatataca cagaggacat
    19141 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    19201 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    19261 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    19321 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    19381 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    19441 cacattatcg ataatattac caaacaacgg agcgagtgct gaagcgacca tcaccatccc
    19501 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    19561 attcgatacc caaagttctg gaccaccttt ttggggaaac attccagcaa attcaccaga
    19621 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    19681 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    19741 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    19801 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    19861 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    19921 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    19981 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    20041 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    20101 ggatgatttc gtttagtttc ttttccattg ctttggcgat tttttttgta taaaaattga
    20161 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    20221 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    20281 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    20341 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    20401 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    20461 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    20521 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    20581 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    20641 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    20701 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    20761 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    20821 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    20881 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    20941 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    21001 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    21061 ttcctacgac agcaaccaca cctagaattg ggattttttg tccagctaat tcacgaattg
    21121 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    21181 gatctaagcc gactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    21241 ggaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    21301 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    21361 aattcaaaag ttcccactca gacatttttt tcactttttc aggcagcact tccttgacag
    21421 ctaaaggaat cgatttgatg caacgggcgt accataatcc ttcaagatta gcaatagaac
    21481 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    21541 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    21601 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    21661 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    21721 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    21781 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    21841 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    21901 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    21961 tgtcttgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    22021 attttttttt tataacatca aatccacgta gatttttaca aacaaaaata aataaagtcg
    22081 gatttttctg ataattcaac gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa
    22141 aatagataac ctaaaaaaag cgaacaaaca aataaaatga ctgtccttca ttcactaaac
    22201 atatttttat ttttttatat tgttaaaaat tcgcaattta ttttttaata gaattatctg
    22261 ttttccaaat tttataacat aaaaaacctt ttttaacggc atttatatgc tttttcagaa
    22321 attaactgat gatttcaaaa agaaaacatg tcataatatt tcccatattg tgcaaaaaaa
    22381 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
    22441 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
    22501 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
    22561 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
    22621 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
    22681 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
    22741 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttatttt aatgactgcg
    22801 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
    22861 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
    22921 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
    22981 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
    23041 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
    23101 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
    23161 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
    23221 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
    23281 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
    23341 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
    23401 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
    23461 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
    23521 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
    23581 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
    23641 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
    23701 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
    23761 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
    23821 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
    23881 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctac
    23941 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
    24001 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
    24061 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
    24121 gctgactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
    24181 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
    24241 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
    24301 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
    24361 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
    24421 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
    24481 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
    24541 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
    24601 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
    24661 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
    24721 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
    24781 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
    24841 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
    24901 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
    24961 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
    25021 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
    25081 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
    25141 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
    25201 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
    25261 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
    25321 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
    25381 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
    25441 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
    25501 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
    25561 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
    25621 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
    25681 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
    25741 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
    25801 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
    25861 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
    25921 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
    25981 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
    26041 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
    26101 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
    26161 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
    26221 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
    26281 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
    26341 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
    26401 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctatcgt
    26461 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
    26521 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
    26581 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
    26641 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
    26701 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
    26761 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
    26821 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
    26881 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
    26941 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
    27001 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
    27061 gcagatgcaa gcttttgaac accgtttacc aagaggttgt gaaaaagctg ccctgcatca
    27121 agtaataaga acacccaagc taaaactgct tcttatgttt ttgacttggt tggacttatt
    27181 accgcagatg caagcttttg aacaccattt accaagaggt tatgaaaaag ctgccctgca
    27241 tcaagtaata agagcagcca agctaaaaca gcttcttatg tttttgactt ggttagactt
    27301 attaccgcag atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta
    27361 gtttcgagta tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga
    27421 cttaataccg agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt
    27481 tttgaaaaca actatttttt caacgtttac tctcttgcca atccaacatc atctaacgga
    27541 tacaactatt tcaatgatta ccattacact aaaaaatcgg aaacacacct ctccgaacac
    27601 aaatacataa gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg
    27661 taagatctcc tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat
    27721 ctgaaaggag catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc
    27781 agaaacgtag atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca
    27841 acagccattt gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa
    27901 gggtatgttg taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa
    27961 acattgaaaa aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat
    28021 ttctgcctga atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc
    28081 gggcttttcg gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta
    28141 agagttcctt atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa
    28201 aaagttttat ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga
    28261 gtaaaaaaag gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa
    28321 aaattcgctg ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt
    28381 ctagataaag cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa
    28441 gctggaacag aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta
    28501 ggaatggacg gattagagcc tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc
    28561 ggcacaatct ctccattaca gatggcatcg caagctgtca aaaaatttgg aacagtccaa
    28621 gtaactggtg tttatatgac tccagcttct tcttacccgc tgcaagaatt ttttatgcgg
    28681 gatatcgctg tcaagcatgg acaggcacca attatccatc taatgccaaa aatttacgca
    28741 atgattgcag aaggactatt cgatcctaca caaatcatta ctcattcgct gccactatca
    28801 gaagcagcac aagcatatga gatctttgac aaaaaagaag ataaaaatat aaaagttgtt
    28861 ttgaaacctt gaattcagta aaaaatacag gtgattttct ttatgcccaa aatcaaacga
    28921 aatacccatg agatcgaaat aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc
    28981 cgcttctatt tccaaaatcg ttacacattg atctcactgg ctgtcgattt attaaccggg
    29041 attttctata tttttggaag ccttgcttcc ttaactaaaa tccccgatcg atatggcacc
    29101 tatttttatt tggctggtgc tattttcttg acgattcgtc ctattctcag aatcgtaagg
    29161 aacattttta tttacgacga aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca
    29221 tgagaatttt tcaaatctca tgcctctttt ttcaccctat cgtatcactt tgatacagac
    29281 gagcggtcgt cccactttct tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat
    29341 aaaacgtggt atgatctgta attaagtaaa gtttttcgta cccaagttga tgcatttctt
    29401 ttctgatttc cgctaatatc cgcttggcta tcccctgctt acgcttttcc ggttcaacaa
    29461 acagggcaca aagatttggt gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc
    29521 ccgctcctcc gacgatggtt tctttttcat ctaagaccac ataccattga ggaatacttt
    29581 ccttattagc gattccagta cgaatacttt cctcatacac aacagcagga actccccatt
    29641 ttgatgaaaa ccaattcgcg ctttgtttta ctaattctgg gtgttctctt aatttgatcc
    29701 agttatatgt aaccaatgtt taaacctccg actaaaataa aacgtttaca aaaattatac
    29761 aagtctttat gaaaattagc aatgacaaca atagtgatgt aaaaaaaaaa cggaaaacaa
    29821 tcacttgttc tccgtttttt gattaatcaa gcatctaaag cttcttttct tctttcttgg
    29881 atcgtagcta ccttgacgaa cggtagatag atcaaaatag aaagaaccaa attgacagcg
    29941 gctagtacac cacctgcaat actttttgtt gccaatacac cgccaataat tggtggagtt
    30001 acccatggag gcataacggt agctgctggc actaaacctg tagaagtcgc aaagtaagca
    30061 acactaacta agaccattgg tgtcaaaata aatggaatga acatgatcgg gttcaacacg
    30121 ataggtaacc caaacatcgt tggttcatta atattgaaga cacctggcgc aatcgacaag
    30181 tttgttacaa ccatatatgg tttgttttta cgtcctacta aataaatagc taacagtaaa
    30241 cctaaagttg cacctgttcc tcctaagttg acaaagctgt caaagaaagg tttgttcacg
    30301 ataaacggtg cgctatgccc agctgcgatt gcattaacgt ttgcttcaat ggctggcgca
    30361 ttgattgttt gcatcaaagg atcgaccata ttcgctccgt gtaaaccaaa gaaccataag
    30421 aatggtgtga taaatgcaag cagtaaggca gatggataag agtttgcaag tcccataaat
    30481 ggttcttgga ccactgtata gaaagaacct acgatatcat caacaccgaa tgcagcaaaa
    30541 attgcagcaa ctaaagcaaa cgcagagata gtgatcattg ctggtagtaa tgaagcaaat
    30601 gcttttgcaa ctgccggtgg cacaccatca ggcattttga tgatcaattt ttcatttccg
    30661 cttaaacgta caaagatttc tgttgaaata atcccaacaa taatagcaat aaacaaaccg
    30721 tttgagctca tacctaatgc tccgcctaaa gcgaagaaag agcctaaaga aatcacacct
    30781 gcggaaacac cgtcttttcg gtagccgtta gctaaatgat aagcaagcaa gaaagcaatc
    30841 aagacagaaa gaatggcaaa tgtaccgttc caagcagcag ccccgaatcc tttccatgat
    30901 tcaccaccaa aaattgagtt catcaattct tggaacccgg gaatcggtaa gttattgatc
    30961 attactgcta atgctcctaa gatcattaac ggcatactca ccataaatga gtcccgaatc
    31021 gcaactaaat gccgttgtgc accaattttt gatgctacag gtataaaatg tttctccata
    31081 aatgcaacaa atttatccat aaatcttccc tcttttcttt actgtagatt acgcgttcaa
    31141 tcgacgatac agatcaatga tctcttttgc taaatctgta aaagctatag atgtcatcag
    31201 atgatcttga ctgtggaccg tcaaaagcgt gacttcaacg tgatttcctt gtgcttcttg
    31261 tgtcagcatc tctgtttgag agtgatgtgc ttggactaaa gattcctctg catctttgat
    31321 tttttcatct gctaaagtga aatcaccttt tttcgcagca gcaatcgctt ccattgcatc
    31381 gctttttgca tttccgccaa acatgatcag acccatgatg gcttctagat ttttttgatc
    31441 ttccatttat tgtatcctcc gataattttt attatttgtt tattagtttt tctgcttggt
    31501 cgagtacttt ttcaccattc atcatgccat agtcagccat attgattaca tctaaaggaa
    31561 tgcctttagg tgctagcttt tcagcaaatt gattttgcat aaagcgtact tgcggtccta
    31621 aaagtaaaac gtctacattt ttagattcta gttgattatc cgcatctgaa gcagatacag
    31681 caaagatgtc tgcttccatt ccttttgctt ctgctgcctt ttgcattttt gtgactagga
    31741 gacttgtact cattccagct gaacatacaa gcatgattgt tttcttttcc attttaaaca
    31801 cgttcctttc tatacttaca cttgtatata agcaagtagc gtgccaactt taaaagcgct
    31861 ttaaaaagtt gatatcatca catttaaggt ctataccaag ttgcatacac actaaaaaag
    31921 aaagcgcaca ctaaacacac aaattaattt ttattacctt ttttctttta acgtgcacaa
    31981 aaaaagttgt gacacaaaac ttgtcacaac ttttttcaaa taaacggtat tcaaaagctt
    32041 gcatctgcgg taataagtcc aaccaagtta aaaacataag atgttgtttt tactttgcta
    32101 ttcttattac ttgatgcagg acagcttttt cacaacctct caataaacgg tgttcaatca
    32161 gctgaccctg ggtattagct caaaacgctg gattcacaac ctcttttatt tcaaataacc
    32221 agtaaataca atgaacatct acaaaaaact tctatttatt tatagatttc gtcaggcaaa
    32281 tccaattctc tcttatcaat caaagtctga taccacgaag cagatttttt caatgttctg
    32341 cttcgttcat cgttcaattc gattcgaact aatccatatc gattcttaaa tgcattcatt
    32401 ggtgacacac agtcagtgaa tgcccataac atatagcctt cgcaatttgc gccttcttct
    32461 gttgctttga tcaagtaatg caaatgctgt ccgataaatt cgatacggta atcatcttgc
    32521 acgattcctt tctcatccat atactgttct tcatattctc ttcccatacc gttttctgtt
    32581 atcagccaag ggatattatg ataattttct tttagataca tggctatatc atacatgatt
    32641 ttcggataga tttcccatcc cctggaatta ttcattttct tcccaggtaa atcaaagact
    32701 tcataatatc gttcaggatg gaaaggtgtc tcttcattcc attgataacg aggggcgcga
    32761 acgcgtttag gataatattg attgattcca aggaaatcta ctgtatgatg ctgaatcgtt
    32821 tctaaatcct ctgttgcagg atcgaaataa atcgattgtt tttgacacaa ctccaggaac
    32881 tcttctgtgt agtatccttt taccatcgga tcaaaaaata ctcgattata aaacaaatca
    32941 tacatataag cagctttttg gtcttctgga gaattggatc gagcatagac catttcagga
    33001 tttaaaatac agccaatcct tccaccataa tttccgctgc ggtaaatctc tactgcttta
    33061 gccgttgcta aaactttatg ataattccac atcatccact tttttgtgtt ttgttcatgc
    33121 ggccagcgaa cagcatccaa atagcaacgc gtttggggaa cgattggttc gttgaatgta
    33181 aaccagtttt ttaccctatc accgtaacga tcaaaggcaa tttgagcata cttagcataa
    33241 aaatcaacta cttttcttga agaccagcca tcatattttt ccaaaagaaa agctgggact
    33301 tcataatgtt ccaaacaaat catcggttct acgtctgctt cgatcaaaga gtcgatcatt
    33361 tcacttatat gctgagcata ttcttcatct actatacagt tctcataatc agtaaaaaaa
    33421 cgagcccaat tgatagaagt ccgataatgt gtcagatgga tggatttcat cagatccaca
    33481 tcctgctggt attgttccat aaaattagtt gctacggctg ggccataccc attatgccaa
    33541 acaaaatgtt catttttata ccagctatct ataaacgaat cctgtccttc tttttttcct
    33601 ttccaacctt ccgtttgcca agcagaagat gcagcaccaa gaataaaatt ttttggaata
    33661 gtaattttca tgatttcctc ctgtttttta gatcccatca ttttccacac gatttgctac
    33721 ttttacaaat ggcagataga tcaaaataga aacaataata caaacaagtt gcgtaataac
    33781 tgcaccgata tttcctgctg ttgccagata agcattgacg atcggcggca ttggccaagg
    33841 gaccattaca actgctttcg ccgcaaatcc taaagcggtt gcaaaataac caatcgttcc
    33901 agtgataaga ggcgttaaga taaacggaat agctaaaatt ggattcagca tgatcggcat
    33961 accaaaaata actggttcat tgatgttgaa gatcccagga gcaagggata acttggtgat
    34021 ttgtttcata tcttcccgtt ttccaactaa caatactgca atcagtaaac cgatcgtgac
    34081 tccagacccg cccatactca tatacatatc ccaaaatggc atagtaataa tgttcggtac
    34141 ttcttttcca gcttcaaaag cttccgtatt tacagcaatt gccgctaaaa gcaacggttc
    34201 ccgtatcggc ttcaccattt gatttccatg gataccaatc acccaaaaga cttgcgcaat
    34261 aaacatcaat agcaaaatac ccggtaaccc ttgtacgaca ccttctaatg ggcgttgtac
    34321 gacattgtaa atgatatcat aaaggtacat tcctgtaagt tctttgatga taaatcccgc
    34381 agccgcgata cccgatacag tcagaatcgt cgggaataaa gcagaaaaac tagcagaaac
    34441 attcgttggc acggtatcag gcattctcag ctgtagtttc ttctgtttcc tcaaccagca
    34501 ataaagttca atagaaagga tggagaccaa cattcccaaa aataaccctt tcgtatctgt
    34561 atattgttta gccaatacgt tttctactat aacagtctga ttttcattca ctaattcata
    34621 aacagttgga ttaacagata aataagacat cactgcgatg ataccaggga atacaccatc
    34681 cactttattc agcttggcta attcgatccc tatcagaaaa acagcataga tggtcaagaa
    34741 acttagtgtg acatagctga tggcagatgc gataggcttt aattcttcta aaaaacgcaa
    34801 gaaacttatc tgtgctaagc cattttccga gtcaaagacc attgctgaaa acaatgtgcc
    34861 aaaagcaccc gtaataatta ccggaatcaa tgcagcaaat gcgtttttta tcaccatgat
    34921 ataccttaaa ttattgattc tcgttgccac gttccctaaa acattggcta atttatccat
    34981 aaaactcatt ccagatcccc tcattcctgc ttcttctcct tgacgtcatc tctttttgtc
    35041 tattagtcag cgcttacatt tcgatgaaca gaaactattt actcctcttt tttaccaaat
    35101 ataaaacaaa aattcatctg atgtatttat cataaaatac atcagatgaa ctgtcaatta
    35161 gtttcttttt ctcctattat tttctgtaat tctctccgat ttattgccat atggacagtc
    35221 attgcatcaa acgcagcaag tgcattccgc ctttcgatgg cctgaacgat ctctgtatga
    35281 atatcgatcg tctcgatttt cagatgctgg atatcataat ttttattaaa caaagcaatt
    35341 gattcattaa tgattgggat aatatgcagc aaggctacgt ttccgctagc ttctgcaatc
    35401 atcgtatgaa aatcgatgtc taattgaaca tgttcttgtg tgccttctcg aaaagaagtt
    35461 tcaatctttt gcgttaattt tttcatttgg acgatttgat tcttcgttgc atattccgct
    35521 gccaaacctg ccatcttagg ctccaataaa tatctcagtt caaataagtc tcgtatcatc
    35581 cgatcctgat cttcaatcaa agaaaaaccg agtgggtcat tggcaatccc tcgcttatca
    35641 ctgacaaatg tccccgctcc ttgccgaact tcaaggatat tcctggaagc tagagcccgc
    35701 acagcttctc gcaccgtgct tcgccccacc tctaaataat cggacaattc atattcattc
    35761 ggcagtttat ctcctacttg caactgcatt tcctttaaat atctaaggat cttgtcagta
    35821 gttacttcta ctagggtttt attttcttcc aaaccacata cctccttttc atctgatgta
    35881 ttttttcgta attattataa cacgtttgaa tcatctgatg attttttgta tacaaaattg
    35941 attaatccgt tcatagacat acaaacgaag aagatattta ccataaaaaa gactaggata
    36001 aaattcactc ctagccttcg atcattttta ttttgaacga caaaaaatat gatgttcaat
    36061 aacagacgat cttcattcga ttcttgatgt catcttttgt tttttttaat atatggataa
    36121 aaagtttgat tacatcgttt ctattctaga atttggtcga ttctcaattc ttcttctggt
    36181 gagaaatcta aattatccat cattcgaaca ctttcttgta actgacttaa ccgacttgct
    36241 ccgaccagta cacttgtaac agatttttgt cgcaaattcc atgcaagggc catttgcgct
    36301 aatgattgtc ctcgtgatac ggcgattgtt tgcagtgctt tgattttttc cagagttgat
    36361 ccaacttgtt cttctgaaag gaatggtatt tccttacgat gtgctctaga atcttcagga
    36421 ataccatgta gataacgatt tgtcagcaac ccttgagcaa gcggactgaa cgtgattgcc
    36481 cctagccctt cttcttctaa tactggagat agatcgtctt ctagtagtgc acgagaaaac
    36541 atattgtagc gcatctgatg aataatgaac ggtgcttcct ttctttttaa aatttcagtc
    36601 attttttttg tatcttctcc attatagttg gatataccaa tatataatgc ttttccttgt
    36661 cttacaagtt gcatcaaagc ttctgctgtt tcttccaaag gtgtatcagg gtctgggcga
    36721 tgatgataga agatgtcaac ataatctagc cccattcttt ggagactttg gtcacaactt
    36781 gaaatgatgg atttacgaga gccccattcc ccatatggcc cttcccacat atgataacct
    36841 gctttgctag aaataatcag ttcatcacgg taggcttgaa aatcttctct aaaaatacgc
    36901 ccaaagtttt tctctgcagc tccagcaggg cctccataat tattcgcaag gtcgaaatgg
    36961 gtaattccca tgtcgaatgc gcctcttaaa atctctcttt gattatggat aggatcataa
    37021 tcaccaaaat tttgccataa tccgagagat aaaatcggta atttcaaacc tgaatgaccg
    37081 gatttacgat attccatttt ttcataacgt gcactatctg caatatacaa tttttctcct
    37141 cctcatcaaa tctttttaaa acgctttctt ttttctattt ttagtttact acattttaaa
    37201 gaaaaggagt taatcaaaat gattttttcg tatatttttt tgccatttgt tttttactcg
    37261 ttgatagatc agaaattgat tacgtgcttg ctggattttt ttataacgtt tcggttgttt
    37321 tttgtaaaat tgctgatgat agttttctgc tggccaaaac gtaccggctg gttcgatttt
    37381 tgtgacgatt ggttggtgga aagttcctga actgtctagt ttctgttttg attgttccgc
    37441 taattctttt tgtcgttcgc cttgataaaa aatcactgga cgatattgct tccctctatc
    37501 ttgaaattgt ccaaatgtat ctgtgggatc agtaatttgc caatagattg tcagcaactc
    37561 gctataactt atttctcttg tgtcataaat gatctccaca gcttccacat gacctgtgta
    37621 accaccactt acttgatcat aatttggttt ttcaacatgt ccgcccgtgt atcctgacaa
    37681 aacagatagg atcccttttt tcgattcaaa cggctcgacc atacaccaga aacatccccc
    37741 tgcaaatact gcacgttctt gatagtcagc atcttgcatg gcatgcttgg aaaagtcata
    37801 tttttgctcc ttatcaaatc cttccgtaat caataaataa aagtccatta catcaggcgt
    37861 cagattcatt cggatagcta atggacgtag tgttgcttct agttttgata actgttcttt
    37921 aacggatata ttttctctta tgtcattctt tgtctgaatc aatacttttc gttcccaatc
    37981 tcgtgtattc ggattcaaaa tcagattata aagtgtttga atgaatattt gttcctcatc
    38041 catagattct cctcctttcc gaagaagaca agctagtgac tttagctgta atttatcata
    38101 aagtgaggaa ataagcgatt acttcatccc cctacagatt tttcgtataa aaaagagaga
    38161 atccttatcg acagattctc ctttttaatc aatttatttt ataaatgaaa agttaattta
    38221 ccttttatgg aaaactattt taaaaaataa acataaatat gcagttaatg taacaagata
    38281 agaaataata gtcgttactg tataacttgt gcttacttta atatcagcaa ctcctttgac
    38341 tccgcttatt tgtaccaatc cattagcatt cacctgctcc tttacaggtt gatgatttat
    38401 ttctacattc gttcctaaat aagcatatac aggaagtgtg attacttggc tcttctcatt
    38461 agttactcta tatatgatag aactaccagt tgttttataa ggaaggttta cccattgact
    38521 gccaatcttt gcttcgtgtt ttattatatg ctccttattc tccaatgaag cgataggaga
    38581 ataatcatat aaaaaataat ccattccttt agtggtatac gcctctactg tttctttgtt
    38641 tacaacaata tgtcccggct gagaaattat tttttgattg aaattttttt tactagaaac
    38701 agttaaagtg attaataaca aaacagtcat aaataaataa aatagttctc ttgtcttatt
    38761 gtatcttcta tttttgaaaa atatcatcgt taatatcaga gaagagaata ttatttgcaa
    38821 gcttaagatc cgccaaggaa attgtaacaa tttggctggc gtattttgca gaagatacca
    38881 tggcaccaaa ctagttgtag ctagccaagt caaacacgta ccataataaa tctttttttc
    38941 tatgtttgtt aattttttaa ataaaatcag gggaatacat aaagataaca gaccaactag
    39001 tcccattgag taagtagtta agtcgttgtt catagcttca gtcagactat cgaacacatt
    39061 agatgcatga gcatataaat ttgtcacgct aggtttgttg attttttggt agagcatttg
    39121 ttcaaacatc ggataccaaa aagcactcgt cagaaccaat gtcattcctg ccgcactaaa
    39181 taatttttta aatatagcaa taaaatctgc attcttgaaa cctttagaaa ccaaacgaat
    39241 aaaaataaaa atgataatta atgctgtact cattaatgtt gatagtacat ggctgtacac
    39301 tagcaaactc atactcaatg ctagctttac agacggatac ttttcttctt ttccagagat
    39361 gatttgataa gcataaaata atacagggat aacaagtgtt attgcgatag cttcggcaat
    39421 tgcggaacga tagtagatgt ctactgtccg ataagcagag aaaatataga gacaactaaa
    39481 taaaaatgcc gctttttttt gttttaaaaa ccgctcgcca taatgataag ctacgagtgt
    39541 tgtacacaag ttcaataacc aaacgtaaat aatatagctt acaatcaaat tttttgtcat
    39601 ccaatataac attacagctg gaaaaaaagt cagaaaggga taaaaataat ttacaccata
    39661 tccagtataa ttgaatgtct cataattaat cggactggta aaaatagtcg ataacccctt
    39721 gattcgagat aagtgaaaag aaagatctcc agtggtataa atactctgtg tatttttgaa
    39781 aaagatgaaa gaatatatac tactaactat aattaatcca ctcaatatta ctactttttt
    39841 atttattttc attcttcttg tttatctcct ttttagtcac atatcgagaa tctttcaact
    39901 tattaggtgc aaatcgttta aaacttgaat acaccactaa acaataagtc agaatactga
    39961 tccataaagc catgagagca gttggagtat attttgttgt aatttctatc ttattttctc
    40021 cagcatttac atccacattc actaagccat gttttttata agagtcattt acgactttac
    40081 cgtttacttt tacctgtgta cctaaataag catatactgg tactgtcact ttttgctttt
    40141 tgttgcttgc cactgtgtaa gtaatgttat tatctgcagc tttatacttc cctttttccc
    40201 acgattctcc tacttttact tcatgttttt ctaagtgagg cctttctttt atcgcttctg
    40261 ttggggcgta atcgtataat cctcccatat ctgatgttgt ataacgtggg agtgtttctt
    40321 cattgataac aatatgaccg tgttttgatt ggataacttt tgcgtaattt tcttttgctg
    40381 ctatagacgt gacgagtaga aacaccacag tagcgccaat gattaaccat gaatattttt
    40441 tattcatagt ctttttcata aaaacaagcg ttatgatcat tgaagaaaaa aagatttgga
    40501 tacctaagat acgccaagga aactggagca agcttatcgg tgttttttga aacaaaaacc
    40561 atggaaataa attagttacg catacccatg tagaaactac gccaacatag atgatttttt
    40621 cttttttctc catttctttc agtatgaata aaggaaaaat caacgcaacc aatcctaata
    40681 accccatagt gtatgttgta atatcgttat ttaacgcttg aatgatacta tctgaaatag
    40741 aaagtgcttc tagctgcaaa tctgttttga agggactgtg tatttcttga taattcatct
    40801 gctgcaacat aggataccaa aaatagctag ttaatgcaca agtcaaaaaa acagccttta
    40861 ctaaattcac gaccacaaat tttatatcgt tttttatttg cttgcttaaa aacaaacgaa
    40921 ctataatcat acaagcaatc aatgcactac acaatagtgt agttaagaca tgagtataca
    40981 caagtagact tatactgaca gataaagcga tgactttttt ttctctattg aatatcaatc
    41041 cataagcata atataaaaca gctgggataa tcgtaagagc gattgcttct gcaattgcgg
    41101 atcgataata gatatcaact gttcgataag ttgcaaaagt atataaacaa ctaaataaaa
    41161 aagcagcatc tatcttttta aaaaactttt ctccataata aaaac
//
LOCUS       DACEPD010000014        39031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.014, whole genome shotgun sequence.
ACCESSION   DACEPD010000014 DACEPD010000000
VERSION     DACEPD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.014"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..272)
                     /locus_tag="IW797_000744"
     CDS             complement(<1..272)
                     /locus_tag="IW797_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223537.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
     gene            complement(310..447)
                     /locus_tag="IW797_000745"
     CDS             complement(310..447)
                     /locus_tag="IW797_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ1223538.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            complement(449..895)
                     /locus_tag="IW797_000746"
     CDS             complement(449..895)
                     /locus_tag="IW797_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223539.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRTDGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLNNVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(896..2962)
                     /locus_tag="IW797_000747"
     CDS             complement(896..2962)
                     /locus_tag="IW797_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAQ1223540.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNEVLAKVEAVSKNVSSAQNDLTILEDRIDQTNQQIGDLGKLKKMYSNSIDFGG
                     YDYSGNPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGK
                     TYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTAN
                     VNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWLGKAP
                     LGENIANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSG
                     DYRYGNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGD
                     TRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKGLNDAVNVYDPQRVEGLKNFAD
                     GIQIAGDKVISENDCTVYTLTKDNSQSFIDGYATFIKHGKEVIVNGTVKFKKAYAFGV
                     PLDDEVPDEFIARIVHGMLVGSSGGNSVAKAMYVRKDLGTIVTNSDFVVNEWFTFNGH
                     YWVGGK"
     gene            complement(2962..3744)
                     /locus_tag="IW797_000748"
     CDS             complement(2962..3744)
                     /locus_tag="IW797_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223541.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPGGIEDDSF
                     VEMMVFADHDLRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTASVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNESAGMTLIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNDVKITKIEGVK"
     gene            complement(3761..5518)
                     /locus_tag="IW797_000749"
     CDS             complement(3761..5518)
                     /locus_tag="IW797_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAQ1223542.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYTDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            complement(5493..8324)
                     /locus_tag="IW797_000750"
     CDS             complement(5493..8324)
                     /locus_tag="IW797_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1223543.1"
                     /translation="MSETIYFFDHLQKLIKRKNKRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPSETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPAVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPEATQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTELLKNTYSTLIDSARPLVQFKAEITGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPGVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            complement(8321..9088)
                     /locus_tag="IW797_000751"
     CDS             complement(8321..9088)
                     /locus_tag="IW797_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ1223544.1"
                     /translation="MYSFKDTTINLNSSKRFLPTSAMMYDGMYLEDLIEGYQTLTVEG
                     REMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELLNYLYRNE
                     DVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVSDGYIGNP
                     IQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQVTVNGEDC
                     TSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            complement(9121..12240)
                     /locus_tag="IW797_000752"
     CDS             complement(9121..12240)
                     /locus_tag="IW797_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ1223545.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEEQMSRVKAISGATGKSFDELRQQAVD
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTIQEAPGKVADWIKNKWTETKEFFSNLWSSIANSASEMWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMIWIDLKLFFTNLW
                     IDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLIKSWNSL
                     KQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNVIQGWKNLKQGTIDTFNNLVNG
                     AQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKSVQDFVG
                     GIGDWIREHKGPIQYDRKLLIPAGQAIMNGLHKGLMGGFNDVQNTVGSMADFIAELFN
                     ANPDVDIAANLKNANKNIGAQVEHKVNMGGSTKPAVFKFNLGRQSFRLFLDDIAQAMG
                     EGADINLEF"
     gene            complement(12257..12544)
                     /locus_tag="IW797_000753"
     CDS             complement(12257..12544)
                     /locus_tag="IW797_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223546.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            complement(12598..12966)
                     /locus_tag="IW797_000754"
     CDS             complement(12598..12966)
                     /locus_tag="IW797_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223547.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFIDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEEETITLDEMDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQAE"
     gene            complement(13012..13524)
                     /locus_tag="IW797_000755"
     CDS             complement(13012..13524)
                     /locus_tag="IW797_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HAQ1223548.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELNLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            complement(13535..13927)
                     /locus_tag="IW797_000756"
     CDS             complement(13535..13927)
                     /locus_tag="IW797_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HAQ1223549.1"
                     /translation="MIKTRDQSIFDELFKISQEKLGYKTYDYKPLDNVGYPFVEFENT
                     QTIHEANKTDIKGTVIVVLSVWGLQKKRKQVSNMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            complement(13924..14295)
                     /locus_tag="IW797_000757"
     CDS             complement(13924..14295)
                     /locus_tag="IW797_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAQ1223550.1"
                     /translation="MKVSLGYKGVDELLKHLEEAVTLRDVQMVVKTNGAELTKRMQEK
                     ARFNGHWEGDVFVHPTGFTRRSIRMWLLEGGFVAQVGPQSDYSPYLEYGTRFMSAQPF
                     VGPAFNVQKAIFMKDMQRLFK"
     gene            complement(14292..14618)
                     /locus_tag="IW797_000758"
     CDS             complement(14292..14618)
                     /locus_tag="IW797_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223551.1"
                     /translation="MRYDTEVTFVIEKDGYYDPELGEHVEPTLDEKIKLANVTDLGTD
                     RSKVLFGDIKQGAKVIRLLRPYTKEWDYVLIFNKLKSKTEKFEIITERNLRLKNTFIV
                     QEVIAK"
     gene            complement(14615..14953)
                     /locus_tag="IW797_000759"
     CDS             complement(14615..14953)
                     /locus_tag="IW797_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HAQ1223552.1"
                     /translation="MDETLEEVKRSLEVDNEELDKQLTDFIKRISSQLCVRLGFLESV
                     PAALNYIVVECTIKRFNRKGNEGMSSYGQEGESISYGKLLDDFEEDIFAYLKKQNGDT
                     PPYRSVATFI"
     gene            complement(14993..15199)
                     /locus_tag="IW797_000760"
     CDS             complement(14993..15199)
                     /locus_tag="IW797_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223553.1"
                     /translation="MTKYKVAKVFIDVHTKETYKVGQEIDLNKERFDEIEKNLEAFGG
                     GFLEPIKIEEKQVKKPTKSTKKKG"
     gene            complement(15210..16217)
                     /locus_tag="IW797_000761"
     CDS             complement(15210..16217)
                     /locus_tag="IW797_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HAQ1223554.1"
                     /translation="MANIAELFSQRNVLDYVNNRQAPPLLGETLFPARKVQGLEFDIL
                     KAGTRIPTIASVHAFDTEAEIASRVASRSAQELAFIKRKIQLKEKDLIALRNPRTAEE
                     QAFLENEVYNDVFSMVSSVNARVEKMRMEVLANGTVTLDENGLDLVVDYGVPSEHKAT
                     VDFAASSTDVIGLLTTWAGTLDTTPTRILTSTKVRNAILQNAGIKAYFKDAGLLPTAG
                     TLNQLLQQFGLPIIATYDAKYYKENAQGVLVKERYFPENKLVMFGDENPGESIFGVTP
                     EESRLLSGGTNDYQVGNIFAMVYESNLDPVGTWTKASGTALPSFPEADNVFQATVLAE
                     G"
     gene            complement(16242..16595)
                     /locus_tag="IW797_000762"
     CDS             complement(16242..16595)
                     /locus_tag="IW797_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223555.1"
                     /translation="MVYVKKSETFKEINFLKSQQFISFTKQVDHTHAGVVNGVLPAGS
                     VYPANDGKAEGITINDVDVSNGAQPVGVIIDGHILIERLPVKPSDAAQKAMREIKLYD
                     ASGKMIALPAAASGL"
     gene            complement(16607..17248)
                     /locus_tag="IW797_000763"
     CDS             complement(16607..17248)
                     /locus_tag="IW797_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAQ1223556.1"
                     /translation="MKKRLFMPMSLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENTTGKTFSRDEVAKMIAAETKKAVTAAEEKWRAEKDEAAKLAEMDDKEKADYEKQQL
                     EAKLAEFERKEVLSKMSEQASEMLSEKGATPTKEMLRLIVSEDAETTSNNVKTYLASV
                     EAEREAIKAEYEKRLGGKVPLDGNGATISRGAQLAKNANNQTKKPENDPWAIK"
     gene            complement(17363..17545)
                     /locus_tag="IW797_000764"
     CDS             complement(17363..17545)
                     /locus_tag="IW797_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223557.1"
                     /translation="MPDMENEEAIVRAIASLNQILQNEKVLRVSVEIDSEWEDGIKSF
                     DISVKYLPSGDINGAK"
     gene            complement(17883..18836)
                     /locus_tag="IW797_000765"
     CDS             complement(17883..18836)
                     /locus_tag="IW797_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAQ1223558.1"
                     /translation="MKSQDYFIKREKAWQEQQIKDDKKRMNEIKKRLQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRADEMDVKAFARKAKKYVKEKDFSPTANKELKLYNLTM
                     RVNRLELLKANIGLELIATFNDMDKYFSGELTSAGLKELQRQAGILEMTIAKSGYAKL
                     VEQVINSSFRADGFATFSERLWMYQAELKADLDKLLVRSVTMGRNPKQLAPELSKFLT
                     EEGRENTKFNTQRLMVTETTRVQTGIQEQSYRDADIDQFVFISEPTACRYCLPLNGKV
                     FDLKDMSPGVNCAPMHPFCRCSTAPYVDREAFEKSLKERGL"
     gene            complement(18841..19104)
                     /locus_tag="IW797_000766"
     CDS             complement(18841..19104)
                     /locus_tag="IW797_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223559.1"
                     /translation="MKARKKPVTVEIVQLKMLSARSYRKCKEFVGEAWVDHNNMPNGL
                     PGIETLEGTMEISDGDYIIKGVHGEFYPCKPDIFLETYEIIEE"
     gene            complement(19117..19347)
                     /locus_tag="IW797_000767"
     CDS             complement(19117..19347)
                     /locus_tag="IW797_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223560.1"
                     /translation="MNLKEQMMNQYQKKDSEKIKDAIAEAMKIGRNEVLYGKDVITDD
                     IRKEFQDGGFTVEDYEDKHTIDAKIELVRFSW"
     gene            complement(19301..20935)
                     /locus_tag="IW797_000768"
     CDS             complement(19301..20935)
                     /locus_tag="IW797_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ1223561.1"
                     /translation="MEQNIQLLGQQRFDEEANLVYKVPVSQLPKIDMLDQQTGEVSEF
                     INFKHEDMWKMIVGFIKHHRERQVPRLKELKRYLNADNNIKRRPNKPDGRADNRIASD
                     FANFIVSFKQGVLLGNPISYNGDKVIVERINRFASESNEDYHNQLMSRDAFGLGRAYE
                     WVGRDEYGKETIAKFDAEQTFVIYDNTKDRNSICGVHYFVEKFLDKSFTRIELYTNCG
                     YNYYFTAKDDDLENAVLDEDGEVQSYFDTVQINEWINNEERTSDFEHVMDSIDAYDLS
                     RSEMANFQQDSSEAYLVIKGNPDTADDQEGDNSKLAVFQAMMQARMLVLGDKKIYDNN
                     VAGAEPDAYYLKKEYDVAGMEANDSRTVADILRFTSLIDFTDENIGSNQSGIGFRFKG
                     WGSDNDRKNKERMVKKAIMRRLRLLTHSWSIKDGLDKPRGLIDTVKAFFVSDEKQQEQ
                     LYNKVNEIQIQFTPNVPQSDEEIMSVIAGMVGIVSDQTLCEMAERLTGVPFEEELKRL
                     KKQAVSGVFDSDKETDTEVEEDESQRTDDEPVSEEG"
     gene            complement(20946..22244)
                     /locus_tag="IW797_000769"
     CDS             complement(20946..22244)
                     /locus_tag="IW797_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAQ1223562.1"
                     /translation="MAKKLSEFLPPKFHSVWRATLNQDILNIVCKGGRGSGKSSDIAH
                     IVTQLLMRYAVNAVGIRYVDNTLEQSLYEQMKWAIEQQGVSHLFKFNKSPLKITYLPR
                     GNYMIFRGAQNPERIKSLKDSKFPFAIGWIEELAEFKTEDEVTTITNSLLRGELDDGL
                     FYKFFYSYNPPKRKQSWVNKKYETSFQPKNTFIHHSTYKDNPFISKEFLSEVEAARAR
                     NPRRAEWEYDGKAIGSGVVPFDNLRIEAGSITDEMVANFDNIRQGLDYGYATDPLAFV
                     RWHYDKKRNCIYAIDELYEVKCSNRRAAQWIKSNKYHYQDIIAEVEPKSNAEMRNEHD
                     ISKIRQVTKGPDSVEYGEKWLDDLDAIYIDPIRTPNIAKEFENIDYQTDRDGNPKPRL
                     EDKDNHTIDATRYAFNDDMRKQPEPVNVKKTIDTFKKLGL"
     gene            complement(22237..22692)
                     /locus_tag="IW797_000770"
     CDS             complement(22237..22692)
                     /locus_tag="IW797_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAQ1223563.1"
                     /translation="MSKLNPKQQAFADEYIITGNAYQSALKAGYKENYAKNAQEKLVE
                     KGGKVSDYIQEKLKEVQTKRHLTMEEALAITASIAKGEPQRFEVVKRDPYTNEIIERE
                     VSEYSAGFKERNQALEHYYKINAAFVDKQKVEISEIPTFIDDISSDDDG"
     gene            complement(22718..22942)
                     /locus_tag="IW797_000771"
     CDS             complement(22718..22942)
                     /locus_tag="IW797_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223564.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIVEMTREAT
                     PNEIDKCKLIYCGHGYFSEQNIQTNIKKYH"
     gene            23441..23620
                     /locus_tag="IW797_000772"
     CDS             23441..23620
                     /locus_tag="IW797_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAQ1223565.1"
                     /translation="MYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQTINSI
                     KNGITEDSQVIDMTK"
     gene            complement(23613..24026)
                     /locus_tag="IW797_000773"
     CDS             complement(23613..24026)
                     /locus_tag="IW797_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223566.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQIKYQGMDRKRLIDII
                     EQQRLELIQKEAVCKEYKKHLEQVIEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTP
                     VFNSVKSEKLESISTALVEKAAEMNSLANEVSRLI"
     gene            24317..24592
                     /locus_tag="IW797_000774"
     CDS             24317..24592
                     /locus_tag="IW797_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223567.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            complement(25050..25463)
                     /locus_tag="IW797_000775"
     CDS             complement(25050..25463)
                     /locus_tag="IW797_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ1223568.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(25540..25836)
                     /locus_tag="IW797_000776"
     CDS             complement(25540..25836)
                     /locus_tag="IW797_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223569.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            complement(25833..26390)
                     /locus_tag="IW797_000777"
     CDS             complement(25833..26390)
                     /locus_tag="IW797_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ1223570.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            complement(26387..26806)
                     /locus_tag="IW797_000778"
     CDS             complement(26387..26806)
                     /locus_tag="IW797_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ1223571.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            complement(26803..27048)
                     /locus_tag="IW797_000779"
     CDS             complement(26803..27048)
                     /locus_tag="IW797_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223572.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            complement(27008..27364)
                     /locus_tag="IW797_000780"
     CDS             complement(27008..27364)
                     /locus_tag="IW797_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ1223573.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            complement(27364..27669)
                     /locus_tag="IW797_000781"
     CDS             complement(27364..27669)
                     /locus_tag="IW797_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223574.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            complement(27666..27827)
                     /locus_tag="IW797_000782"
     CDS             complement(27666..27827)
                     /locus_tag="IW797_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ1223575.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            complement(27824..28126)
                     /locus_tag="IW797_000783"
     CDS             complement(27824..28126)
                     /locus_tag="IW797_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223576.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            complement(28288..28557)
                     /locus_tag="IW797_000784"
     CDS             complement(28288..28557)
                     /locus_tag="IW797_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223577.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            complement(28569..29318)
                     /locus_tag="IW797_000785"
     CDS             complement(28569..29318)
                     /locus_tag="IW797_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1223578.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(29321..30007)
                     /locus_tag="IW797_000786"
     CDS             complement(29321..30007)
                     /locus_tag="IW797_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223579.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            complement(30013..30684)
                     /locus_tag="IW797_000787"
     CDS             complement(30013..30684)
                     /locus_tag="IW797_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ1223580.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(30677..31018)
                     /locus_tag="IW797_000788"
     CDS             complement(30677..31018)
                     /locus_tag="IW797_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223581.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(31196..31894)
                     /locus_tag="IW797_000789"
     CDS             complement(31196..31894)
                     /locus_tag="IW797_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ1223582.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            31981..32451
                     /locus_tag="IW797_000790"
     CDS             31981..32451
                     /locus_tag="IW797_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223583.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(32494..32673)
                     /locus_tag="IW797_000791"
     CDS             complement(32494..32673)
                     /locus_tag="IW797_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223584.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(32660..32998)
                     /locus_tag="IW797_000792"
     CDS             complement(32660..32998)
                     /locus_tag="IW797_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ1223585.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(33014..33217)
                     /locus_tag="IW797_000793"
     CDS             complement(33014..33217)
                     /locus_tag="IW797_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1223586.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(33232..34008)
                     /locus_tag="IW797_000794"
     CDS             complement(33232..34008)
                     /locus_tag="IW797_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ1223587.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            34307..34627
                     /locus_tag="IW797_000795"
     CDS             34307..34627
                     /locus_tag="IW797_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1223588.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            34645..35076
                     /locus_tag="IW797_000796"
     CDS             34645..35076
                     /locus_tag="IW797_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ1223589.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            35206..35667
                     /locus_tag="IW797_000797"
     CDS             35206..35667
                     /locus_tag="IW797_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAQ1223590.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            35701..36336
                     /locus_tag="IW797_000798"
     CDS             35701..36336
                     /locus_tag="IW797_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ1223591.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            36449..37084
                     /locus_tag="IW797_000799"
     CDS             36449..37084
                     /locus_tag="IW797_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223592.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            37383..38522
                     /locus_tag="IW797_000800"
     CDS             37383..38522
                     /locus_tag="IW797_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1223593.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(38591..38664)
                     /locus_tag="IW797_000801"
     tRNA            complement(38591..38664)
                     /locus_tag="IW797_000801"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38628..38630),aa:Arg,seq:cct)
BASE COUNT        11089 a         8179 c         6353 g        13410 t
ORIGIN      
        1 aaagttttaa tttgttgcgt gtgttctacc aatttttctg catgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtgacttact tttgaaagag tctcagtaat cttcgagaaa
      181 gatgcagtaa ttggttttat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcgctc cccattcccc taaattaatc atgtgacaac tccttgaatc aaaataaaaa
      301 gcacatcaat taagatgcgc tctcttcttt gctaatgatt ttatctgctt cttcgtctgt
      361 aatgcatagt ggaacgaata gtcgaacttg atcgtcagta aaacagcccc aatcatacat
      421 cattttcaca tcgctaaaac taaacatact actcacctcc ctttgaagct ggatttagtt
      481 gctctttaat ttctgaaatg tctttgctat tttgtaacga agcaagcatc atttttgaat
      541 tgatttgtgc taaactatcc gctttttctt tcaatgcagt attttcctgt ttaattgcta
      601 cattgtttag catgagtttg gcgttgagct gttttaaatc gccgttctca ttttctaacg
      661 actcatacat cgctttgaga ttgtttaaat cgttgtgatc tagtacattc actaaaacaa
      721 tccattggtt cagtttagga tcaaacatct gatcagcaat cgttagcggt tcgccatcag
      781 tacgaattcc ttcgagtggt ggaacatcgg taaatggaac agtcataacc atatcatcaa
      841 gcacttgtcc agcatactct ccacctgttc gtccatattt ccaaatgttt ttcatttatt
      901 tccctcctac ccagtaatga ccgttgaatg taaaccattc gtttacaaca aaatcgctat
      961 ttgtcacaat tgttcctaaa tcttttcgta catacattgc ttttgcaacg ctatttccgc
     1021 cagaagatcc cactaacatg ccatgaacaa ttcttgcaat aaattcatct gggacttcat
     1081 catcaagtgg tacaccaaaa gcataagctt ttttgaactt tactgtacca tttacaataa
     1141 cttcttttcc gtgtttaata aatgttgcat acccatcgat aaacgattga ctgttgtctt
     1201 tagttaatgt ataaacagtg caatcatttt cactgatcac tttatctcct gctatttgaa
     1261 taccatcggc aaagtttttc aaaccttcaa ctctctgagg atcatacaca ttaacagcat
     1321 cattcaagcc tttttcagta tattcaggtg tgacatccca actgtaatca ttgggattgt
     1381 tactgtcttt caagccttca ccaaagtatt taaactgact aatattcggg gttcgtgtgt
     1441 cgcctttttc gatcttgagc cagtcaattt gacatgcacc tgttgttgat tggggatact
     1501 ggataatagt taaccgtttg gggtagttaa ctccaacctt ctgcggtgta aaagtcagac
     1561 cccatgtatc aactaaccct tctactggct ctaagttacc atatctataa tctccactat
     1621 cctcgttgta aacaatgaac gtttggatag gtggtttggt tgccttcatg gtaattgtat
     1681 aggtttgacc taaaacaaat tcttcttcca tgttagcttg gtataggctg taggcgctag
     1741 atttgattgg aaacgtaaca gacttattag caatattctc acccaaaggc gctttaccta
     1801 gccagtaagg gtcatcaagt aagtttggct gatatggggt ggctgttgag ccttcttcga
     1861 ttttgatgtc gtacaactta aatccgccat ttattttatc cctgtcaaca aagctaatac
     1921 tcatataaaa tcgatctaaa tttgtgattt gataattaac gttagctgta cctttgattg
     1981 ttatttcttt ccctacatca tctgttgtaa tatttgtact atttgcttcc aataatatct
     2041 tttctcctgg tgatgtacga tacaccaaac gtaatttatc aatagctcct gtagttcctt
     2101 catcaaatcg aactttcgca ctcagagtat aggttttccc actagcaagc tgaggtgtat
     2161 taatacgcgt aaacataata atgcttcctg taccatcaga agtaaaatgt agcttttcgt
     2221 tatccgaagt tagtgacccg tggtaaccaa cgttaaaatc gctcgatttt agtttggaca
     2281 ttaaattcgg attcccacta taatcatagc ccccgaagtc gatgctgtta ctgtacatct
     2341 ttttcagctt gccgagatcg ccgatttgct ggttcgtttg atcaatacgg tcttcaagta
     2401 tagttagatc attttgcgct gaactaacat ttttagaaac agcctcaacc tttgctagta
     2461 cctcatttac tgcatctgtt gcttcttgtt tgactccatc aagtaattgt tgaaagtctg
     2521 caataaagta gtctgcttta tcttgcgcta gtccgtcgat tgctgaacgc ttcatacgga
     2581 aggtgaagcc taagttatca cttgttgatc cgtctggata ctcgatgtaa acataagctt
     2641 ccaccatacc gttataagct ttcatcgctt ctggcaagat atatacgacc tgtccattca
     2701 agaagctttc agtgatgagg tttttcgtga taaaaggaat tggctctttc gtgatcattc
     2761 catctacttc atcatagata aacatcaaca agcgcaagtt agctcctagc aagtccgccg
     2821 gtgtgccgtt ctgttgttcg acgttgaact ctaacgctat ttgattatcg tatgatttaa
     2881 atacaagccc cgttgcttgc aaatcatagt ctttcggctg tgtaggtact ttgatagagc
     2941 cttttttgat gacatgcgcc attatttcac accttcaatc ttagttattt tcacgtcgtt
     3001 gctcattgcg ggattggaaa cattgccgga tgaaatatct aaagctctgc cgtttgacat
     3061 cgttatcttt cgcgcttcga ttgtcagttc aaactcgatc aacgtcattc ctgcactctc
     3121 gttccataaa ttagcttttg taatccgcgc gtatctttga cgtttcactt cttctacaaa
     3181 gtctccgtca ccgtctgtgt agtggatacg aagcgtttgg taacggaaag tatcatctgg
     3241 cagattgact gattgtcctt ttcttaattc tccttcaaaa agaacagaag cagtgtaaac
     3301 gcgccgccaa cctaacgagt tataaccctc taccggttcg ctgttatgtg tcattttgat
     3361 gtaaatttcg ccgctataac gagcaaaagc gacgattaat tttctcaaat cgtgatccgc
     3421 gaataccatc atttcaacaa aactatcatc ttcaatcccg ccggggttgt ctgcacccca
     3481 tccggctttg gttgcgtatt ttccgggtgg aatatccaag atgttcggat atttttgtgg
     3541 caacttataa tcgcgtaacc aacgcccacg cgcttgcata actgtttctg gtgtagcaaa
     3601 gcccgcgttg ctttcagtag ctaggacgtg cgcgtcgtga tccgagccgt cgtaatggat
     3661 agccgcttga cgcaataacc atttcacagc ctcttcataa ctcatttctt caaaaatatt
     3721 tggtttgctt tgaaaatcta acatttaatt gttcaccgcc ttaatagtta taagtgaagt
     3781 ctctcgcgcg ccctacgcct ttgcttgtca cttttcttgt tgctgtgtca acttctattt
     3841 tataaaacgc taattcatcc ggtgtatcaa cttggctagc tgtatgagag aagccgacgt
     3901 cgcaaagaat catttttact gtcccaaacg ccccttcgac ttgttcgtgc caatgcccgc
     3961 agaaataacc tactacaacg ccggcgcctt ttgtattcat tgcgaaggtc tttaatccgc
     4021 cgaacgaacc gtcattcggt tgtcctaatt tagaccaatc gatagttacc ggactaccag
     4081 atttaaaccc ttctatcaac gtgctaatca tgttttcatt gcgaacggga aacttgctga
     4141 gacctaaagg cgtatgtcct actaacgcca catgatagtt tctcggaacg ttcactaacc
     4201 attctccgaa agcgttaatt tgtttggcac tgaaagctcc cggcgctgta tcgctgtaac
     4261 cgtccgtgta cttgtcacct gtgccgcctt cataaaaatc acaagtatcg aaacggtaaa
     4321 ttgctgcgtt tttatctggg aacaacacgc caccgtacaa accgttccaa tactcttcga
     4381 agtcagcgtt gcataacatt ccctttcgct tgcgccacgc ggggtcgaaa caagcgtcgt
     4441 gattcccttt gcagataata accggttttt cttgtcctgc taccgctgcg ttagtgaagc
     4501 gtttgagcgt gcctaacatg gaatgtcgcg cgctccattc atcaataatg cctatatcgc
     4561 tgccgagtga accaagtccg ccgtcgatat tgtctccgcc gtaaatcatc acatctgttt
     4621 tgttccctaa tttttgaaat tgagggattg cgcgccaatg tcttaaataa gaagcatctt
     4681 tgtaaccgac gccatctata cgcaagttgt gattatctgc gtgaatgtct gtaatgaaag
     4741 aaaagttaaa tttgctgtta ttaacactgt tgacaaccgt attcaaattg cgcggcacta
     4801 ggtcaacgta tttcatcgtg tcgtaatcaa aaaagcgtgt tgtttctcta atttgtgagc
     4861 tgccgactgg cacttggtat ttttggttta aacgatcagc taatgattcg tagtcgcctt
     4921 tagcttcatt cagaatgtta atgatcatac cacctggatc gatattttcc agtatttcac
     4981 gattatcttc taaccactgc tcccagtcat ttttgccctg atccatgtaa tctttgaatt
     5041 ttcttagcaa atcctcaaat gtccacacat agccagagtc acgtaactgg cttctagata
     5101 ttccagaaat gactcgatag gtaaaatcct gtgtgctaaa ttgttcactc caagttccat
     5161 caccattaag tgatcggaaa ctgaaatgtg cggtgttttc accaccccat tgccagtcag
     5221 gctcacttaa ggtgtaaaca agccttgctt gcgctggact gtattcttgt actttttgtt
     5281 caacaggttg gttttcgcca aattttgttg tattaataaa aaacggcact aggccctcga
     5341 atgtttttag tttgccatgt tccaccactt caacaacgaa cttttgcgtt aaaacatccc
     5401 cttgccgaat tcgaaccaaa tttattccgt tgtttggttc ggtggtggat aggaccattt
     5461 tatgctgcgt ttctgccatg actatccctc ctttagaaat cgatgtagtc tcttgcattg
     5521 tggaaatgac ctgaagaaga tggataaaac tcatccataa attgaaaatg aagatgttct
     5581 ccagtggatg gtcctgttgt ccccatcagt ccaatttgct gaccagcagt taccttctga
     5641 ccttttgaga cgtctactcg gctttgatgc gcgtatcctg tatacattcc atcagcgtgt
     5701 ttaatcactg tccaatttcc ataccagtca aagtaatttg catcacctgc aacaatcact
     5761 tcgccatccg ctgaagcaaa aataggtgta ttaggatttc catttacaag gtcaataccg
     5821 ttatgaaatt cttgtgctcc tgtgattgga gaagtgcgcc agccaaattc gctcgtcact
     5881 gtgattggat ccgcaatcgg cttcatatat ccttttgata caggaatttc caaatctttg
     5941 aacttgtcat accattcttg tgaccatgcc gtccgttctg gatgtggatc acgtggacgt
     6001 tcaaagttag ccacgaatgc ttgtgctgct gtgttgatat cggtcagatt catgaattgt
     6061 gtccatgtgt aaggataagc gctagttgcg atccattgac catttggtgc atgccacatc
     6121 aacaatttga actgtgccgt gattgtgtct ggatcatcac tgatgccagc ctttgtcatt
     6181 aaattgatca tgtagacacg tccgctagtt gcgccagaag aatcggtcca ttgccataca
     6241 ccataaccga accctggtgc gccattgccc tcatcagcgg ttggattagc atctgattct
     6301 ccttgtgcat ttccaagtaa ggctgcagca gcttgttgtg taaaaccggc acctattgcc
     6361 gttgcccaaa tttgccaata tcgtttatcc cgatcagtag ttactcctgg tgggtattgt
     6421 ccgttccaac cgttatcgtt ccctccggaa ttgcctcctc cattagtgtc aatttttgta
     6481 ccgttaacat agagttcttt tacatctagc cgtccatcta tggttatatt tccttctgaa
     6541 aaattgccgt caccataaag attatatttg cgcttatcag cagtaacatc tgctggaatt
     6601 tgaaaaatag gattccctcg atcgccaccg tccccagcat taatggaaaa aatatagttt
     6661 ggctctttcc agacagcaaa gccgttaatc cctttagaac cataagtaga tacaattgag
     6721 cccaagcgtt ctccatgtac atcatctagt ccagtagact tgacttgttt ttcaaaagaa
     6781 agttctcccc cctctgccac taattggaaa tctttatcgt caaatgtttt taaagctacc
     6841 ccttgcacga ggataccaga cagaatgcct gcgcgaataa aattagcatt gaaagttcca
     6901 tccaacgtcc aagcggtcgt gctatcacca ttgtgtacat cttgaattgt ttgccactca
     6961 ccttttttac attgtttgaa agatatccct gagttatttt ggaccataaa aaagcgtgat
     7021 ctaggaatgt taggtccatc catataaaca gtttcataga tttctctact atcactaaca
     7081 ccagcttcaa ttccatttac ccaataaata gaaccgccat tatctcctgc acctcgcata
     7141 atgtcatctt gatattttcc aatctctgtc gattcgtaaa atgtcatttt gctagattct
     7201 aaactattaa tattattgac aatagaagcc gtttgttttc taacatcttg tgttaaatta
     7261 tcccctagtt cgatattcgt ttgaccggta agccgattga atgtagtttt ataaatacga
     7321 gttttatagt gataaccttt atcgtatctg tgaatagtca ctgtatttcc tatcacatct
     7381 cctccagtga tttcggcttt gaattgtact aacggtctag cagaatcgat taaggttgaa
     7441 taagtatttt taagtaattc tgttggatca tctatatcat caaacactac cacggtttct
     7501 cgctttctca ttgatccatc tttttgtggt ataccatact tttgagttgc ttccggatct
     7561 tcaagccaat tttggccttt aggcttatct aaaggatcac cattcgactt tttccattca
     7621 acatcagtga attcaattct tctaccgtat ccgtcaccaa cctcttcgcc tcgcccgcga
     7681 cctatcattg aagttgagat ggagcttcga tctatctctc ttacaactgt taatgcttta
     7741 ctaccatata caaaacgtgt attcgattct tcaccaattt gttcatatac ttcgatccat
     7801 ttatccttta ttccatcaga attcaaagaa cacctaaaga caaattccat acctaaggtt
     7861 tgcaattctt tcaacgcttc ttttacagag acatagtaaa aagttgcagt tactgctggt
     7921 aacattgctt ctacgtgacc aacgcgccaa tttccttcag taaattcaat caatcgatca
     7981 agaacatttt ttaagggctg cccactcggc ctaatatctt tgatgatgta agcatctaat
     8041 tcatttgtcg caaatcctaa ccctgtaaac tctaatgttt cagatgggtc gctcacttta
     8101 gtaattcgat acaacgaaaa agacgactcg ttttcacgaa tcgccatata tcttgcatct
     8161 tctatttctt tatcatattt tgtcgtaacg taaagagtat ctttcattag atcactctta
     8221 tcagaactaa tttctttttc ttgggagact tcaattaaac ttcttttatt ttttctttta
     8281 ataagttttt gcaagtgatc aaagaaataa attgtctcac tcaaattgtc gcccccctat
     8341 agaatatttt aagcttccca ttattgctag ttatcttctg accttgctta agataaaagt
     8401 tctcaaaatc actttctaaa tcaatcatag aagtacaatc ttctccgttt acagttacct
     8461 gctcatcgga aaaatcaaaa accaacacgt ctcctgtttt tattgccgca tcagttatcg
     8521 tgatattttg ttctccgttt gtaattttga ttgaattatt catggataaa gtaacttcaa
     8581 tttttcttgg tgttatagga aactgtattg gatttccaat atagccatca ctaacacatt
     8641 ctttcgtata ctttagtgga tccgcacaga atacattaaa actcgaaata atcgagttgg
     8701 agtctcctgg aacagtatca gttgatgtat agcgaccgta gtaataataa tctaattcat
     8761 catgaaacct aatttccacg tcttcattcc ggtataaata attcaacagt tctttgaatt
     8821 taaactgtaa tttttctgga tctctgtctt ctaacttgta tgttattttt agtgttcttg
     8881 aaggtatttt ctgatttgta atgattgaac caatttgtat ctcttgctgt tcaacttcta
     8941 cagaaagcat ttctctacct tccaccgtaa gcgtttgata accctcaatc aaatcttcta
     9001 aatacattcc atcgtacatc atggcagacg ttggaaggaa tcgtttagaa ctattgagat
     9061 taatagttgt atctttgaat gagtacattt tattttctcg ctgatccaaa atattccctc
     9121 ctaaaattct agattaatgt ctgcaccttc gcccatagct tgtgcaatat cgtccaaaaa
     9181 taatctaaac gattgtcttc caagattgaa tttaaataca gctggtttag tagagccgcc
     9241 catatttact ttatgttcaa cttgtgcacc aatgttttta tttgcatttt tcagatttgc
     9301 agctatatct acatcaggat ttgcattgaa aagttccgcg ataaagtctg ccatacttcc
     9361 aacagtattc tgtacatcat tgaatcctcc catcagacct ttatgcagac cattcataat
     9421 agcctgacca gctggaatca atagctttct atcgtattgg ataggtcctt tgtgttcacg
     9481 aatccagtca ccaatacctc caacaaaatc ttgcacagat ttccatgcat tttgtaaccc
     9541 ttctagaaaa ctatccatga tagcttttcc ggctgctagt aaatcgatat ttttcaagtt
     9601 atcaaaccag ccagttactc tatcaaccgt atcactaaca gcatttacta aattatccca
     9661 tgcctcttga gcaccattta ctaaattgtt gaaagtatct atagtgcctt gttttaggtt
     9721 tttccaacct tgaataacgt tatctttggt tccagtaatt aaatcaccaa tccaagattt
     9781 gaatgaagtc caaatatctt tagcaccttg aaccgtatta ttaaataaat ctatcgttcc
     9841 ttgctttaag ctgttccaac tttttataag agtttgcacg atattattaa ccgtttggaa
     9901 aaaccattgt tttagattat tccaaagttc aattgcacca tattttgtat caatccaagt
     9961 ttgaattatg gagaatttta gatttatcca catttgaatt gcactgtatt taatatcaat
    10021 ccataaatta gtaaaaaata gtttcaaatc aatccaaatc atagtcgctt gataaactac
    10081 ttcattccaa atattagaaa tactttgtat gaatccagtc cataaactaa cggcactatt
    10141 tactattgca ccgatatatt ccgtaaatat attttttaca gatgtccaaa tatttaccac
    10201 tgattggaca agtgtgttcc agattagatc taaatcctct ttcatttgtt cgaagtcacc
    10261 agtaattaaa tctataataa acagtagcgg cgcagctatt aaagacttaa tgatttccca
    10321 agcatttact attatgtttt taacctcagc aaatatactt gttattgctt tcactatatt
    10381 agagaacaca tctgaaaagc tacttacaaa tggaccaata tattttagta taaaatcaaa
    10441 accagatttg attttacttg taattgtttt ccatgcagta gatatagtat ttttaaaccc
    10501 ttcccatttt tcaccagcac ttgaaactaa atcatcaata actgatatga caccttcttt
    10561 taaactattc cacatctctg aagcagagtt tgcaatactt gaccataaat ttgaaaagaa
    10621 ctcttttgtt tcagtccatt tattcttgat ccaatctgcc actttcccag gagcttcttg
    10681 aattgtagtc caaacattgt ctgcgccttc tttaatggac ttccataaat tgttaaacca
    10741 ttctcctgta gatttccatg cattctgaat ccattctact gccgagctta cagcagactt
    10801 gattccctcc cataagccaa tccaaaagtt tctaaaatct tcactcgtat tccaaagata
    10861 gatgaaacct acaacgagta gtgctaccgc agctataacc agaaaaaccg gatttgtaag
    10921 aagacccatt gctttactta atccactaaa caattttgaa ctttcactta aagctttaaa
    10981 agctactttc accctattga tccatataat tactgaacca atcataaaaa ttaacggtcc
    11041 tatagcagcg acaattgctc ctatggccac tactaatttt tgagttgatt ctggagcact
    11101 tacaaatttt tctactaagc cggatatagc atctgctact tttttgatgg atggtgctag
    11161 aatcttttga attacaatag ctgctgactc aaaagctcca aacatttgct cgatggaaga
    11221 attcatatta tcttgcatgg tccgagccat atcgtcagct gcaccatcag aatctttcag
    11281 agattttgtt aatttgccca atgaatcagg tcctttatca atcaaagcca tcatccctga
    11341 taatgattct tgcccatata gtgttactaa agcattttgt tgttgttcag gcgtcaggcc
    11401 ttcaaaagct tttttaagta attctacttg agtttttaaa ggtttcattt taccgtcagc
    11461 atcataaaac gaaacaccta aattatccat tgtatcttgc atagcctttg ttggccttgc
    11521 taacctagac aatgctcctc gcaacgttgt acctgcttga gaacccttaa tacctgcgtc
    11581 actcataata ccaatagctg ctgcagtttc ttccaaagaa atacccattg aattggctac
    11641 aggagcaaca tacttcaatg cctctcccat gtctccaact tcagcattgg tatccgcagc
    11701 agcacgagca aatacatcag cgacatgtcc tgcttcactt gcttctaaac caaatcctct
    11761 caaagcagta gcagtatttt cagaagctaa agccacatcc cctccagata cagctgctaa
    11821 gtctaaaaga cccggcattg ctttcatgat ttcttgtgcg ctaaatccag cagaagctaa
    11881 gttttccatt ccagcagctg actccttagc gctaaatgct gtcttagcac ccaaatcaac
    11941 agcctgttgt cttaattcat caaaagactt acctgttgcg ccagagatag ctttcactct
    12001 actcatctgt tcttcaaaat cgccaccaac tttagcagct gctacgccta ctcctataag
    12061 aggagtggta atatactttg tcattgcggc accagtacct tgcatcactt taccaacggc
    12121 ggttgtcata ctatttgaat tcttttcaaa agttttaaca gcatcttgtg catctttaaa
    12181 agtctttaca aatccactat ctgtggcttt taataaggct tcaacagaaa attgttccat
    12241 gattttcctc ctttcctcaa gagttagctt tagttagtaa gctttggaat tttttatctt
    12301 gttttgaaag ttcggaaact cccatgattg aatcttcgat tttttgataa ttaaagaatt
    12361 cttcaaagga tcgatataca ggaactgtct ttttgcctac ttttttctcc gcttggactt
    12421 gctgatttgc ccacgctaat tcgtgaatca acttttcttt gtcaagccaa gataactggg
    12481 ctgcagtcat acgaatgttg tattcatata acgtcattct ttcgatatct gagatattgg
    12541 tcattcccaa atatcgaaaa gaattgataa gaatttgttc gtatgccagt gcagaatcta
    12601 ttccgcttgt tgtttttccg cttctttcaa tttctgattc aggtttcgga ccgctaactt
    12661 tcccgcgttt gactccgcca attcttttag gacttcatca aacaattttt cgatgtcttt
    12721 aacttcatcg atgtaatcat ccatttcatc caacgtaata gtttcttctt ctgttctatt
    12781 tgctatttct aagactcgtg acaacgtgtt gacattataa gaacgtaatt ccggtaagac
    12841 ttttgctgag agtcccattc cgaattccat atttccatcg atgaaaggca tcactttgtc
    12901 taattcacgt acaaatttag tgccaaattt aaacgaatat tctttacctt taatttttaa
    12961 tttcaatgtt tttcatcctc ctaaaataaa aaagagagca tctaagccct cttatgctcc
    13021 tgtcgaagtt gctttcacgg tatctttgaa tgcatattga acaacatcgg cttgatcttc
    13081 tgtcaaggtt gcataaccat cttggccaac accatttact gcaaatgata aatttaattc
    13141 aacgttatcc tctgcagcag ccgatggagt aaattcagac acatatgctt ggtaataagt
    13201 agctttgtac ttatttgcat tatcatctgt tccctgttct gctttgttga tttcccaaat
    13261 ttcaatgata tcgccattta ataaggcttg tttcatttca tctacatgag aatctccttt
    13321 agcaactatt gaagtagccg aaaaatcata ttcaaccggg cttaaacttt gaacgtttcc
    13381 gtcttttgtc actgtagagt ctgaatctct tgataagcca ttttcgtgtt ctgtttgaaa
    13441 tgccattttc caagcagctt cctgagtttc ttttttcaat aagcgataaa gcaaaatgac
    13501 atcaatacct tttaatgctt ccatgttctt cctcctatct aattctaaat tcaagtgtga
    13561 caaccgctcg ttttaggggg gtattggttg ttgtgtcgtc catcacttga attccacttg
    13621 cttgataatt taaagcccaa taatagcctt ctgtggcttc tatcaatcta gcttcattaa
    13681 aaagagcaga tgccatattt gacacctgct ttcgtttctt ctgtaatccc cagacggata
    13741 aaactacaat cacagtacct ttaatatcag ttttattcgc ttcgtgaatc gtttgagtgt
    13801 tctcaaattc cacaaaagga taaccaacat tatctaaagg cttgtaatca tatgttttgt
    13861 atcctagttt ttcttgggat attttaaaaa gttcatcaaa aattgattga tctcttgtct
    13921 taatcatttg aataacctct gcatgtcttt cataaagatt gctttctgca cattaaatgc
    13981 gggaccaacg aaaggttgag cgctcatgaa tcgagttcca tattctaagt acggactgta
    14041 atcactctgc ggtccaactt gtgcaacaaa accgccttcc aacaaccaca ttcgaattga
    14101 tcgtctagtg aagcctgtcg ggtgcacaaa cacatcccct tcccaatgtc cattaaagcg
    14161 ggctttttct tgcatgcgct tagtaagctc ggctccattt gttttaacta ccatttgcac
    14221 atctctaagc gtgacagctt cttccaagtg tttaagcagc tcatccacac ctttgtaacc
    14281 caatgaaact ttcactttgc aatcacctcc tggacgataa aagtgttttt aagtcgaagg
    14341 ttacgctcag tgataatctc gaacttctcc gttttgctct ttagtttgtt gaatattaaa
    14401 acgtaatccc actccttggt atagggtcga agtaaacgaa taacttttgc accttgctta
    14461 atatctccga acaaaacttt cgagcgatca gttcccaaat cagttacatt agcaagcttg
    14521 attttttcgt ctaaggtcgg ttctacatgc tcccctaact ctggatcata atagccatct
    14581 ttttcgataa cgaaagttac ttctgtgtca tatctcatat aaaagtcgcc acacttctat
    14641 aaggaggggt atccccgttt tgcttcttta gatatgcaaa aatatcctct tcaaaatcat
    14701 ctaaaagctt tccataggag attgattctc cctcttgccc atacgagctc ataccttcgt
    14761 tacccttgcg gttgaatctc ttgatcgtac attcgactac gatatagttt aacgccgcag
    14821 gaacgctctc taagaagcct aaacgcacac atagttggct tgagattcgt ttgataaagt
    14881 cagttagctg tttatcgagt tcttcgttat caacttcgag cgatcgtttc acttcttcta
    14941 aggtttcgtc catgactgcc tcctttcaaa aataaaaagg ctagtcaaat gactaacctt
    15001 tctttttagt tgatttagtt ggtttcttaa cttgtttttc ctcgatctta atcggttcta
    15061 agaatcctcc accaaacgct tcaagatttt tctcaatttc atcaaagcgc tctttgttca
    15121 aatcaatctc ttgaccaact ttataagttt ctttcgtgtg aacatcaatg aaaactttag
    15181 ctactttata tttggtcata gaggatcacc tacccttctg ctaatacagt tgcttggaac
    15241 acgttgtccg cttcggggaa gctaggaagt gcagtaccag atgcttttgt ccatgttcca
    15301 acagggtcaa gatttgattc ataaaccatt gcaaaaatgt ttccaacttg ataatcatta
    15361 gtaccaccag ataatagccg agattcttct ggcgttacac cgaaaataga ttcgcccgga
    15421 ttttcatcac cgaacatgac aagtttattt tctgggaagt agcgctcttt gaccaacacg
    15481 ccttgagcat tttctttata gtacttagca tcgtacgttg cgattattgg caaaccaaat
    15541 tgttgtaaca actgatttaa tgttccagct gttggcagca atccagcatc tttgaagtaa
    15601 gctttgatac cagcattttg caaaatagca ttacgaactt tagtcgaagt taagatccga
    15661 gttggtgtgg tatctagcgt acctgcccat gtagttaata atccgataac atcggtagaa
    15721 gaagcagcga aatcaacggt agctttgtgt tctgacggaa caccgtaatc aacaactaag
    15781 tccaatccgt tttcatctaa tgttacggtt ccgtttgcca atacttccat gcgcattttt
    15841 tcaacacgcg cattaacaga agaaaccatt gaaaatacat cattatatac ttcattttcc
    15901 aagaaagcct gttcttcagc agtacgagga ttgcgcaatg caatcaagtc tttttccttc
    15961 aactgaattt tgcgtttgat gaaagctaat tcctgagcgc tgcgagaagc tacacgagat
    16021 gcaatttctg cctcagtatc aaatgcatgt acgcttgcga tcgttggaat acgagtacct
    16081 gcttttagaa tgtcaaattc aagtccttgc acctttcgtg ctggaaaaag tgtttcaccc
    16141 aacaaagggg gagcttgacg attgtttaca tagtctaaga cgttacgttg tgaaaataat
    16201 tctgcgatat ttgccattta ttttttcctc ctatattcct attacagtcc gcttgcagcg
    16261 gctggtaaag cgatcatttt cccagatgca tcatataatt tgatttcccg cattgctttt
    16321 tgagctgcat cgcttggctt aaccggcaac cgttcaatta gaatgtgtcc atcaataata
    16381 acgcctactg gttgagcgcc gttactaaca tcaacatcat taattgtgat gccttcagct
    16441 ttcccatcgt ttgctgggta aactgaccct gctggcaata caccatttac aacacctgcg
    16501 tgagtgtggt ctacttgttt tgtgaatgaa atgaattgtt gagattttag aaaattgatt
    16561 tctttgaatg tttctgattt tttaacataa accattgata ttcctcctat ttaattgccc
    16621 aagggtcatt ctcaggcttt ttcgtttggt tgtttgcatt ttttgctagc tgtgcaccac
    16681 gagaaattgt tgcgccgttt ccatctaaag gaacttttcc gccgagtcgt ttttcatatt
    16741 ccgctttgat tgcttcacgt tctgcttcaa cagatgctag atatgtttta acgttgtttg
    16801 atgtggtttc agcatcttct gacacaatta gtcgaagcat ttctttcgtg ggcgtagcgc
    16861 ctttctcaga cagcatttca cttgcttgtt ctgacatctt agacagcact tctttacgtt
    16921 cgaattcagc tagttttgct tcaagttgct gtttctcgta atctgctttc tctttatcgt
    16981 ccatttcggc aagtttggca gcttcgtctt tttctgcacg ccatttttct tcagcagcag
    17041 tgacagcttt ctttgtttca gcagcgatca ttttcgctac ttcatcacga gaaaatgttt
    17101 tgccagtagt gttttcttct tttgcttcgg gcggtgtctc ttgtgagcca gctggtaggt
    17161 ttccttgttg tccctcatca ccagatccac cgtctcctgg ttcagaaaaa aattgtaagc
    17221 tcattggcat aaataaacgt tttttcatga ttaatcctcc acggttacgc cgctacccga
    17281 taatttaact agttacgcca gtcagtcgga acagctttct ctttagcgcc tgtaagcagt
    17341 aagaaggcat aataaaaagc cgttatttgg ctccgttaat atcgcctgaa ggcaaatatt
    17401 tcacactgat atcaaacgat ttaataccgt cttcccattc actatcaatt tcaactgaga
    17461 ctcttagcac tttttcgttt tgcagaatct gatttaagct tgcgatagct ctaacaattg
    17521 cttcttcgtt ttccatatct ggcatctcct ctggatcata ataaaaagcc gttagctaat
    17581 gggctagcga ctttgtaaca tgctctgatt catattgttt tgcaaagctt tgaagtactc
    17641 tgggttagac tgaaccaatt cggttattga ttgcacgaca ggttcaataa cctttctagc
    17701 agcttcatat acttctttta cgattggttc taagacatca attaagtgct gcttaacggc
    17761 atcatagtct tctttaaatt gagcatcgtt ttgatacaaa tgcaaaattt attctttgct
    17821 ttttccgtat gctgtttcta aaaatctgtc agatataact tgaaatgaat ctttctccat
    17881 ttttataacc ctctttcttt aagtgacttc tcaaaagctt ctctgtcaac ataaggtgcc
    17941 gtactacatc tgcaaaatgg gtgcattggc gcacaattta cacccggcga catatctttc
    18001 aaatcaaata ctttcccatt tagaggcaga cagtacctac aagctgttgg ttcagatata
    18061 aatacgaact gatcaatatc agcatcacga tagctttgct cttgaatccc cgtctgcacc
    18121 cttgtagttt ccgtaaccat caaccgttgt gtattaaact tagtattctc tcgtccttct
    18181 tctgttagaa acttactcaa ttctggagcc agctgtttag ggtttctacc catcgtcaca
    18241 ctacgtacaa gcaacttatc taaatcagct ttcaactctg cttggtacat ccatagtcgt
    18301 tcgctaaacg ttgcaaatcc atctgctcga aacgagctgt ttatcacttg ctccactagt
    18361 ttggcataac cgcttttagc gatcgtcatt tctaggatgc ctgcttgacg ttgcagttct
    18421 ttcaaaccag cgctggtaag ctctcccgag aagtatttat ccatatcatt aaaagtggct
    18481 atcagctcaa gtccaatatt tgctttaagc aattccaagc ggttaacacg catcgttagg
    18541 ttgtatagtt tcaactcctt gtttgctgta ggtgagaagt ctttctcttt aacatacttc
    18601 ttagccttgc gagcaaatgc ttttacatcc atttcatcag cacgcttcat tgcttcgcta
    18661 cgagtgattt tctgcccgtt ggaaaaacta tcccactgtg cgtctatctc tttctgtatc
    18721 gcatcctgtg cgtattgcag acgcttcttg atctcgttca tgcgtttctt gtcatcttta
    18781 atctgttgct cttgccaagc tttctcccgc ttgatgaagt aatcttgtga tttcacttaa
    18841 tcactcctcg atgatttcgt aggtctcaag aaagatatct ggtttgcacg gataaaattc
    18901 accatgaacg cctttgatga tgtaatcacc atcagatatt tccatcgtgc cttctaatgt
    18961 ttcaatacca ggtaatccat tcggcatatt attatgatca acccatgctt caccaacgaa
    19021 ttctttgcat ttgcgatatg aacgtgcaga tagcatttta agttgcacaa tctcgacagt
    19081 aactggtttt ttacgtgctt tcatttctta tcctccctac caagaaaatc tgactaactc
    19141 aattttcgca tcaatcgtat gcttatcctc gtaatcctca acagtaaagc cgccatcttg
    19201 aaactctttg cgaatatcat ctgtgataac gtctttgccg tacaggactt cgttcctgcc
    19261 aatcttcata gcttcagcaa tagcatcttt gattttttcg ctatccttct tctgatactg
    19321 gttcatcatc tgttctttga gattcatctt cttcaacctc cgtatcagtt tctttgtcac
    19381 tgtcaaacac accagaaacg gcttgtttct tcaatcgctt aagttcttct tcaaacggaa
    19441 cgccagttaa ccgctcggcc atttcgcaca acgtttgatc tgatacgatg ccgaccattc
    19501 cagcgattac gctcatgatt tcttcgtcag attgcggtac gtttggcgta aattgaattt
    19561 gtatctcgtt tactttgttg tatagctgct cttgttgctt ttcatcagaa acaaaaaagg
    19621 ctttgactgt atcaatcaag cctcgtggtt tatccagtcc gtctttaatg ctccaagagt
    19681 gtgtaagcaa ccgcagacgg cgcataatag ctttcttaac cattcgttcc ttgttcttac
    19741 gatcgttgtc tgaaccccaa cctttgaaac ggaatccgat gcctgactgg ttggacccga
    19801 tgttctcgtc agtaaaatca ataagagatg tgaagcgtaa gatatcagca actgtccggc
    19861 tgtcgttagc ttccattcct gcaacgtcat actctttctt aaggtaatac gcatcaggtt
    19921 ctgcacctgc aacattgttg tcgtatatct ttttatcgcc taatacaagc attcttgctt
    19981 gcatcatagc ttggaatact gctaacttgc tgttgtcgcc ttcttggtcg tctgctgtgt
    20041 cagggttccc tttaatcacc aagtaggctt ccgacgaatc ttgttggaag ttagccattt
    20101 ccgatcgtga aaggtcgtat gcatcaatgg aatccattac atgctcaaaa tcacttgtgc
    20161 gctcttcgtt gttgatccat tcattgatct gaacagtgtc aaaatagctt tgaacttctc
    20221 catcttcatc cagtacggca ttttccaaat catcatcttt agctgtgaag taatagttgt
    20281 atccacagtt tgtgtataac tcgatccgag tgaatgactt atccaaaaat ttctcaacga
    20341 agtaatgtac gccgcaaatc gagtttctat cttttgtgtt gtcataaatc acaaatgttt
    20401 gctccgcatc aaacttagct attgtttctt tgccatattc gtcacgtcca acccactcat
    20461 aagcacgacc taagccaaat gcatcacggc tcatcaactg attatggtag tcctcattag
    20521 attcgctagc aaaccgatta attcgttcaa caataacctt atcgccgtta taactaatcg
    20581 gattacctaa caagacacct tgtttgaacg acacaataaa gttagcaaaa tcgctcgcta
    20641 tgcggttgtc tgctctcccg tctggcttgt ttggacgtcg cttaatgtta ttgtctgcat
    20701 tcagatatcg ctttagctct tttaatctag gtacttgtct ttcacgatga tgcttgataa
    20761 agccaacaat cattttccac atatcttcat gcttgaagtt gataaactcg ctaacctctc
    20821 ctgtctgttg atctaacatg tcgattttcg gcaactgact aactggcacc ttatagacaa
    20881 ggttagcttc ttcatcgaat cgttgttgcc ctagaagctg aatattctgt tccactgtat
    20941 cacctctaca atcctagttt tttgaatgtg tcgatcgtct ttttgacgtt gactggttct
    21001 ggttgctttc tcatgtcatc gttaaaagca tatcgagtag catcgattgt gtgattatct
    21061 ttgtcctcaa gtcgaggttt tggatttcca tcacgatctg tttgatagtc gatgttttcg
    21121 aactctttag cgatgtttgg tgttctgatc ggatcgatgt atatcgcatc taaatcatct
    21181 agccattttt caccgtattc aacactatca ggccctttag taacttgcct tatcttcgat
    21241 atgtcatgct catttctcat ctcagcgttt gatttaggtt caacctctgc gatgatgtcc
    21301 tggtagtgat acttattgct cttaatccat tgagctgcac gacgattgct acatttaacc
    21361 tcgtatagtt catcaatggc atatatgcaa ttcctcttct tgtcataatg ccaacgcaca
    21421 aaagcaagag gatctgttgc gtaaccgtag tcaagccctt ggcgaatgtt atcgaagttt
    21481 gccaccattt catctgtgat acttccagct tcaattcgta gattgtcgaa cggtacaaca
    21541 ccagatccaa tagctttacc atcatactcc cactcagctc gtctagggtt ccttgctctg
    21601 gctgcttcga cttcgctcag aaactcctta gaaataaaag ggttatcttt ataagttgaa
    21661 tgatgaatga atgtattctt aggctgaaag gatgtttcat attttttgtt tacccatgat
    21721 tgtttccgtt ttggcggatt ataactgtaa aagaatttat aaaaaagacc atcgtccaac
    21781 tctccacgta aaagggagtt cgtgatagtc gtgacttcat cttctgtttt aaattctgct
    21841 aattcttcaa tccaacctat agcaaatgga aacttgctat cttttaaaga cttgattcgt
    21901 tctgggtttt gagcaccacg aaatatcata taattccctc ttggaagata agtaatttta
    21961 agtggtgact tattaaactt gaataggtgg gaaacacctt gctgttcaat cgcccatttc
    22021 atctgttcat acaatgattg ctcaagtgta ttatcaacat aacgtatacc tacagcattc
    22081 actgcatatc tcataagtaa ctgagtaacg atatgcgcta tatccgatga ttttcctgaa
    22141 ccacgcccac ctttacaaac tatattaaga atatcttgat ttaaagttgc tctccatact
    22201 gaatgaaact tcggcggaag aaattcagat agttttttag ccatcatcat cactacttat
    22261 atcatcaatg aaagtaggta tttcagaaat ttcaactttc tgcttatcta caaatgctgc
    22321 gtttatttta taataatgtt caagtgcttg gttacgttct ttgaaacctg ctgaatattc
    22381 actcacttca cgttctatga tttcgtttgt ataaggatct ctcttaacaa cttcaaagcg
    22441 ttgtggttct ccttttgcaa tagaagcagt aatagccaaa gcttcttcca ttgttaaatg
    22501 cctcttagtt tgaacttctt ttagcttctc ttgaatgtag tcggatactt ttccaccttt
    22561 ttccaccaat ttttcttgtg cgttcttagc gtagttttct ttatagccag ctttcagtgc
    22621 tgactgataa gcattgcctg tgatgatgta ctcatcagca aaggcttgtt gcttaggatt
    22681 caacttactc attttccatc accacctttg ttatatgtta atgatatttt tttatatttg
    22741 tctgtatgtt ctgctcacta aaatatccat gaccgcaata aataagtttg catttatcaa
    22801 tttcatttgg cgtagcttct ctggtcattt caacaatgga gtacttcttt ttaatctgga
    22861 ctgattggac aactctaatt ggatcatctg cgtttggttg cggatattta tttgataatg
    22921 atacatacca gtagttcctc attatgcagt ctcctttacg caaaataaaa agaccactca
    22981 acgagtgatc taatatgtaa aaactacatc tcaacaatga gatgcagtta tagctctatc
    23041 aagcaaccta caccgattcc atcgattact atcgacctcg ccttgctcgt gtactttgag
    23101 cgcccatttc caaccctcgg ttgctaaaat cactggcaag gaatcgaacc ttgcaacacc
    23161 attttccgat attttacaga ctctccatca taaaaactct cacctctaga attgtctatc
    23221 tttaggggac ttatttcaag atacctcttg ccctttaccc tcgacggtct cttgcctagc
    23281 tgcataaact aagctcaggt gttcattacc actgatttaa gttattagcg ttacctttcc
    23341 gccacagtga caaattaata ttgtgaaaat aaatactaag cgtataattt tagttatcag
    23401 cgagtggtcc gctgaaataa atcaaaaggt ggtgaaaaat ttgtactttg ttataagaga
    23461 agcaactaat ggccaatatt acttcgttat taagtccaat aacaatgaag tagtagcaac
    23521 tagcgaaaca tacttgacta aatattccgc tgaacaaacc attaattcta taaaaaacgg
    23581 aatcaccgaa gactctcaag ttatcgatat gactaaataa gacgactaac ttcatttgct
    23641 agggagttca tttcagctgc tttttctacc aaagcagttg agatactttc tagcttttct
    23701 gatttaacag aattaaatac tggcgtcttg atactaatcg atacatcttg acgaggatca
    23761 ggactcgttg ctctattttt ttgcaccact gatctgtact tttctactga atgatactca
    23821 attacttgtt ctaaatgttt tttgtactct ttacatacag cttctttttg gatcaattct
    23881 aaacgttgtt gctcaataat atcaatcaac cgttttcgat ccataccttg atactttatt
    23941 tgttcgactt ggtcactagc cttgttgagc agttctttta gttcagtcaa atctaatttg
    24001 acagttactt tgatttcttt atccattcgt ttttcctcca atacataaat taatagacag
    24061 caacaaaata acattgcttt gataatttgg tatcgaccac tataaatttc ttttcttgca
    24121 attattttta atatatgcta gattatcaac cgatatagtc actgcctgta ctagcggaaa
    24181 ctagtgcagg ttttttgttc tatttactca aaagttatta cgataaattc ttattgtgaa
    24241 aataaatact aagcgtataa ttttatttat cagcgagtgg tccgctgaaa tataaaacaa
    24301 ggatgtgcaa gacaacatgt ataaacccta catgattagt tatgatctta ataacccagg
    24361 gcaaaagtat gataaagtat ttgaaataat aaaagaattt ggggcatata taaagctaca
    24421 aaaatctttt tggttagtta aaactaattt gaatccaaat caaatgtgtg aaaaattaaa
    24481 tacagtactt gataataacg attccttatt catctgtgaa ctgcaaaaaa attatcaagg
    24541 tagagctaca gaggaaaatt ggaaattcat taacgaacat attttctctt agtaaggatt
    24601 agatattttt tctctgttta aacaattgca aataccgtca atatctgaat tagaaacact
    24661 taccttttgc tccttgctac taccaatagc ttggagcaat tcttgtattt cttctggagt
    24721 gccttctact gataatttca tttttcttcc ctccaataca taaattaata gacagcagca
    24781 gttgaaagac gacaagaaca tgtttagttt tgtgagtgct gctgtctaat caaaataaac
    24841 agcaaccgat gaagaattta ggaggagttg aattcacatc cttttcttca taattagttg
    24901 ctgtctatct aagaagaagt ttaaaacgat gagggagatt gcctcccttc gtttattttg
    24961 tcgatcctgt ttcctaatct ttcgacacta ccataataac actggtaaat gtctaaaaac
    25021 cgccatcatt ccgccaaaaa accgccaaat tatttataag caattatttt tccgtgttta
    25081 tatgcttctg caaactctat tagagcttcc gacttcatcc gttgtatact tctttctgaa
    25141 taacccactt cacggctaat tctgtagttt gagaagctat ctggcacaca gaagctgtag
    25201 tagagtatct gacgactaat cagactaaga gccatcaaag ccgctagaat cgcatctctc
    25261 tccgtttcta tatccatcat ctgaataatc gcgtcttctg ccttattgcc atgcttcggt
    25321 gccttcggca tatccgttat aatcggcgac ttaatatcta tcaaagagcg acctgccatc
    25381 cgctccaaac gccgaaagtt cttcagcaca tctctcgcat tacatcttgt ctgtttgaaa
    25441 tctacctctc gtaacaattg catcaagtca aaccgctcct ttatgtgata taataaatgt
    25501 gttggattta ttgaatcagt cggagcgatc cggctttttt tatttgctct tgattacttc
    25561 catgtcaact aatctcgcta cagctaaata atgcttgtgt tttgcagtcg aacgatcaca
    25621 cttcattgta ttttcaatac gaatgattgc tgagtttgac agtatctttt taacgtatcc
    25681 tctgaacgga taaacgaact cttctgcttc acagcggacc atgtcaccga ctttgaattt
    25741 tgatttcttg cgtgttttag ggttctttgt cggcatatct agcattaaac cgccgatacc
    25801 gtgactgcta gcgtaaaatc cgtcttttag tttcatcttt ctaccacctc ttccactgtt
    25861 ccattcacca acaatgctgc agcatccgct gttgctttat cagtgaaaag caatcgatga
    25921 gatggattgc attcaaaact ataatttact ccatcttttc cccataaagc acatttagta
    25981 agatagtttc catcggcatt ctttattgcc cataacggct ctttctcaac ctcgtagccg
    26041 ttgtataggc tcaatagtgt ttcatatgat tgtcttgttg cccaagcgag taattcttct
    26101 ccttgctctt gcgtcactat gccatctcgt acaaaccaat cacagaaata atatgaatca
    26161 ccatctttag atttgattaa atacgctatt ttttcagctt ttgtaaaagg gtccgtagat
    26221 ttctcaagcc aaccggccac gagttgtgga ataactggtt tctgcggttc gtctattttt
    26281 ttcattaacg taatacctga atttatactc tcgttatagc aagcagctat tgcatcattt
    26341 cctatcgctt taatactttc taactttttg attgcttcct gtttattcat cgctgttcct
    26401 ccaataactc gctattctcg tatatatttc cggtgacttc gtacgtgtat tcttcaaaca
    26461 gctctgtgtt aaaaatttga tactctaagt cttcatcaac agtggcacaa actaatccgg
    26521 aataatctat tgaatttttg acaatacaga ctttattatc taagtaatcg aagccatttc
    26581 gcacgctgac taatactata tccccttcaa atatatccac accattctta tctttcattc
    26641 ctgtggattg catgaggtcg atgtttcgca cgtgatatga tttcaattcg gtaggattta
    26701 tccaatattc aataaagttt gttttgccgt ttttagtaaa atgcaagacg gctacatctc
    26761 tcattacgtt ctttcgttta tcccacgctc taaacttcgg tatcattcgc tgtcctcctc
    26821 gtatttttca atcaattcca ttactttttt cactatttca aactcaaccg cttttgattc
    26881 ttcgaaatca tgaacaattt ctggaaacag tacatcatca actacccaca aaataatctg
    26941 gcgctttcca ccaaaattta tgattagatg gtccgattcc acagatagtg ttgctcctga
    27001 ttcgatatca tataaatcca tgctgaattg aacgagtttt tttatcattt gctgtcctcc
    27061 tcaaatactc ttctaatatt tctttatact tctctacaaa tttgaaacga tcttgatgaa
    27121 gtttcttgct ccaatttgtt tgccgatcca gctcacgcat ctgatcgaac cctttttgaa
    27181 tttcgttgta ataaaattca atgtttgctg ctgctttcca atgcctgcta cttcgcactc
    27241 ctgctcctgt ttcagccatt tctaacttaa ctaattcagc tcgttctttt gattttttat
    27301 ctttctgaat cttcatcatg attttcttga ggatgatgtc actgtattgt gtaatgagat
    27361 ccattatctc tcctccacat acctaaactg tcgtcctttt gaatcaatcc ataagctcct
    27421 agctctatcc cagataatgt ttttgcttaa tccagtaatt tcagataact gttcagcagt
    27481 acctgttact aaaattcggt catcatgcca gattgcaatt tttctcggcg ttctccgttt
    27541 gggcttttca gtccacattg atttaccgag cttttggact tctgcaacta tttctttgtc
    27601 ttcttgccaa gattctgact tggttaattc agcaattctt ttcattgctg ctttcttatc
    27661 cacgctcatt cctccaatcg atggatttcc cttcttaaat tctctatgtg caaatcgatt
    27721 gcctttctcg ccgtttcatt gaccatcact gcctttgttc gttccagatc gtcaatctca
    27781 cgctgaaggc ttcgaatacg catttgaatc acttcttctg ttgtcatgat gattcctcca
    27841 cgtatctaaa cgttctcttc ttagcgtctg tgtagccaca ccttgctctt ttcctcacga
    27901 ttttctcgtg caagcctgtg agacttgcta actgttcagc tgtccctatc actagaagtt
    27961 tgtcaccgtg ccaaattgcg atttttcgtg gtcttggctt gttgctcttg tctgcccaca
    28021 tcgctcttcc aagcctcatc acttctgaag cagcttcttt gtcattttgc caatcttctg
    28081 aataagtcaa ttcgataatt cgctgcattg ccgctttctt atccatcccg acattctcct
    28141 ttcagtaatt tgagtacttg atcaagtgcg ctctcacgtc cgccatggaa cgtgttgagc
    28201 cacttgtctt cgtacgatac actttgtctt aaagcttctt gatgcattag ttcaatctgt
    28261 gctgtaaatg tctttaggtc catctgatta cacctgctca agttcattaa gatgtttttg
    28321 caagccttta acacaatcaa caaatagtaa tttggtatat gctaaatttc ttaattgtgt
    28381 tgtatcgata taaagtgcga aatagtatct gagcttactc caactcgaac gatcattctt
    28441 aattccttca attccagctt cttcgagttg atcatatacg tctctcagga cgtctaattc
    28501 ctcgcccttt ttataattcg ctatttcatt aattagttct agataatcga tcttcatttt
    28561 tccacctctt agaatggtgc ttttgattgt ctattagctc gttctagcgc ttttttcttg
    28621 agataggctt cttggtcgat tgcccattca ggaagcttct ctcgtcttcc tgtgcgcttg
    28681 tatccactgc ttgcgttctt aggttcactt ttttctttcc ttgcccaact tcgaatagtt
    28741 gccaaatagt ttttataagt cttaccagat gattcacaat actctgacag tcgttcgatt
    28801 cgctcttggt aatcattagg gaattctgtt ttgagtttct ccatctgctc atctgacaaa
    28861 agaacatttt tatactctcc gtatttatga cggacgggct tagccttcga ttttttcgaa
    28921 ggcggtaact ctcttatata ttcttttgta ttattaaatg tattattaat agatgtatta
    28981 ttatctttga ctttttcgtc aataggggta ttgcgttttt cgtcaatagg gtattgatta
    29041 attcgtaggt acctattgat tatttgattg gtaccctctt tgtaaatgat ttcccgattc
    29101 aagtatccaa acttaatcaa atcacttacc catcgcgata tggtctcttt attcacacca
    29161 tataaatctg caaagtactc attgcctgcc caacaaaagc ctctttcatt acacaaggcc
    29221 gttatctctc cgtataacaa cttagtattt ggtttaagtc ttttgtcgta ccttacgttg
    29281 gctggtataa tcgcataata acttcgatgt tctgtcattt ttaccctcca atatttaact
    29341 ttttgattgt tttctggttt aatttgatcc ctttgatttg atatttattt ttgaaattaa
    29401 tcacacctat tttgtgcttc tctgtgtgat ggattctgca gagtgctgca aatgtgtact
    29461 ctgcatgatc aacttctttg cgctttcgtc ttcctagcgc tttgtcaaag tgatcgatgt
    29521 cagctcctgt tttgccacag atgcagcaga ctctttttgt gatgcatttg tagaagtaat
    29581 attcttgatt cgcaggtaaa atctcatagc cttctttgaa aggaatatga tgttcaaaga
    29641 tgaaatctaa gatgatattt gctaagacat tggcatcact cacagttgta ttcgattcgt
    29701 ctttcaggct tattttgcgc cctgtgacac cttcaaaacg aaagtagaag aattccttcc
    29761 agaagtccgt tggcatgcct gtatcgataa aaatatcgcc tatcagcgca tagatgaagt
    29821 ttcgttgctg tacggtgaaa cgtctaggat caataaaacg aatttcaatg actcgatcac
    29881 catcgtagcc gtcatacatc gtctttagtc gatcaatgtt cacttcctca ttgatggttg
    29941 cgcttatgtc ttttcctttg aactttttca gaaccgctga atatgaatcg attaatggtt
    30001 taaacactca tatcacttct tatctaattc ttttctctta gctgctattg ctcgctccat
    30061 caaggcacat tgctcatagc ttaactgttc aatagtttca acgttatcag ctaagagccc
    30121 taatttatct gtctgctcat taacatattc gattaaggtt ttggtcatat ctttacccat
    30181 ctgctcattg aaagcttcta gaatcgtctc tagcatgttt aatttctttg tatcgattct
    30241 aggtggtgtt ggaatatctt ccccttgaaa tacatataat cccagtccgt gtagagccaa
    30301 tgctttcaca aagcatcgct tcaatgagtt attgatttgc attgcatttg gtttaacaac
    30361 tggttggttt cgataatcta aaacaggaaa taattcggtt tccgtgtgtc ctttaaccgt
    30421 tactgagaca gatacataag tcccagtttc atccataaga aaaggtttat attcctcaac
    30481 aagaaagtct tgatgagttc cagaaacaac cctgtagtgt ttgtactcat taatagttac
    30541 cgttgcctgt ggatcattct ttttcataat ctcccacgcg tgagcccaag ataaataatc
    30601 aaaatttcct tttttcttga gaattttatt taacttacga ctaaaaagtt tttcaaagtt
    30661 cgttgtccct ttgatttcac tcatcaaatt ctgcctccat ttcagcaatg tatttcttac
    30721 ctggtccgta ataagagata tcaatcaagt tatctctgtc gtactcttct agcgcatcaa
    30781 tcaagccatc ttcgatgacg taaatatatt caggtttatt cgaatgcttc gatagatgga
    30841 taagataaac atgatcccaa atagttacat ggttgcctaa atcatcttga tcccaagcta
    30901 gttcttcatt cgtcaaaaga tttcgtctga tttttcgacc acttgtttcc tcaattttcg
    30961 gcttgcccca atcaggatca atcaaatatt gatctagagt ggaaagttct ttttccatat
    31021 gctaaaatct ccttatgatg tgttttcttt gtgactcttt gcttgccggc ggagtcactt
    31081 ttttatttgt tgccatgctt tttgcttgtc aatatgttgt tggcttagga tgattggttt
    31141 atagtatttc caccagcaat tagcaattgc cgtccctatt cttagcgctt cagctctatt
    31201 cattgtcatc accaaaaagt ctctgttgtc tgttcagttg atcaatttcc atacggatcg
    31261 cagtttctgg caaccacatt tcaataaatg aaacagcatc atcgaatctc ttacgaggta
    31321 actcgccata tcttgggatt gaaaaggtac gtttaaattc agaccaaaat tttgagaata
    31381 cttttttgct gatttcttca taagctcggc tttcttttcc ccctagaact cccataactt
    31441 tcatatttcc tttttgctta atttcaaact cttgttgtcc gctaattcgc atagtatctt
    31501 taagcatgga aacatctttt ttaacatctt tcatttcttc taattgatag atcatcatat
    31561 cttcaattgt ttgaggaaca gtattcttgc gaataacatc ttccatttcg ttgaatgctt
    31621 caatgtattt ttgtttgaag tagatagctt tctttcctgt aaaccccata gccagcaaga
    31681 aaaatccatc tctactaatg aagaaaactc gtcgatttct gccatatgaa tctggttcat
    31741 taccttctac aaacatctgt ccaaaattgg acacatcttt ttttagtgca tcaatatctc
    31801 ttaaaacatg ttgatgtttt ttctcgaagc tttctgccac ttgtaagctc gtagtcacag
    31861 cttctttatt tttcaaaatt actaattctt gcattatttc ttctctcctt tttggtataa
    31921 tttagttaaa aactggtggt gtttattttg aatttttttc acttaacaat ttttcaatgg
    31981 atgtcaataa taagttttat attgagtacg tttttaacag ggataaaaat aaaaggaatg
    32041 cgccctcaac tagaagttga acttaatgct tcttacttcg ctgcagatat gatttataca
    32101 aaagtaatca tttctaacta ttcaacagaa ccagctatgt tagttaattt agaactttca
    32161 tccgctaact taaaccgcag atggtcagct actccattta agaaattaat tgctaaagga
    32221 ggatcagtcg atgataatag aatatactct gaagctgtac cgctaaatat tcctcctaaa
    32281 agtgctatat cttgttacct tgcatttgaa gtagggaaaa ttaattttaa taaactcctt
    32341 aatcaccaaa caaaaatgat atttactcta aatagaaccc aaatccacaa aattgttgat
    32401 atcaaaaata caaacttccc aatagagaaa ttagtgaaag aattaaactg agtaatagta
    32461 atatacattc tagtttctct tttctattca tttttagtca gccccctcgg ttggcttttt
    32521 cgctctgtac tcagcttcag cttcatcaag ccccataaaa atccaaacca tgtaaacaat
    32581 cgttcctatc aacgcttgcc tgcttcccca aagtcctaaa gcgtagatga ttagcggtgc
    32641 gctgaacact agcgctctat taaattttcc catccgcttg cctccttaag atttccgaaa
    32701 aatttgtttc taaaaattca agtgttttac ttcttaaaaa tagatatgtg tctcttcctt
    32761 caactggata atagacaaat ccatttttgt ttttctcgat atcgataata tttctatatc
    32821 ttgggttctt taaaactcta gaagtaaacc aatcatattt tctgttaatc cgttctagca
    32881 cttctggcaa cgtcatccat ctaccagtat catcagcttt ttttaactcc tcataatcca
    32941 cttgggagat aattacatag ccttctggaa ttgggatttt tgcttctaga tattgcatta
    33001 gctgttcctc cctttacaat tcgtacatag tgataatcga atctataatt ctgtttgctt
    33061 ctgcagaagt ctttttaccg tttaaaatta aagataagta gcttttgcta attccaaatc
    33121 tttcagcaag catggtgtaa gttaagaact ttgaactttc gacatattct ttgatttttt
    33181 ctctatctcg ttgagtgatt tctgcaatgt cagtcatact aaaactcctt tctaaccaat
    33241 ttcctctaaa tccatttgag ggtaatatcc ttcttttttt agtaattcgt agataaatag
    33301 acgccctttc tgtgtccatt tggtattcat tacaatttta gtgccaccat cggctttcgg
    33361 gatctcagtt gtatgagatt ttgtgtatcc ttggttcatg tgttttttgc acaataacca
    33421 ttggttaccg acttttttct gaatacctag tttatgaagt aatttattca tctgttgtgg
    33481 agacatccca taatctgctg caatctgact aattgttact gaatctgtag aagataatat
    33541 gctatctaaa taggagattt taggttcgta ctctgcaatc ttttgttctg cgattaatct
    33601 tccagttctt tcttctttta gttgagttgc taattgaatg attgtatctg gattaagcaa
    33661 agcttcttct actttttctg gagttagata acctccatgt tttctaattg ctggcaacac
    33721 ttcacttgtt acccaacgtt taaatttttt ggcagaggga agttttgatt ttaagattaa
    33781 actgtacaaa ccagattcgt tgatgattgt catctctctt gattgacctg aggtcgtgat
    33841 tcgcgacccc atcttatctt ccaaatctac atgccgcgac aaagcatctt tagtgtttga
    33901 ataacccaaa acactcgcaa cgtctttgcc tacaaaatat ggttcatcat ttactaaaat
    33961 tgtccgaact tcgttttgtt cgaaattaaa aatttgcggt gtgttcatat ttctcattcc
    34021 tttcttttat attcgtaaac aaatttaaca acttttttct aaattcaatt gacagtatct
    34081 gagtaatact ctatactaag agcataatta aataagacat aaaaacattg attttaaagc
    34141 tttcttggcg gtctgcattt acatatcaat agtgtttttt gttgtctttt tagttgttaa
    34201 atctgtttac aagatatagt atagctcatt actcagaatt agtcaaatta tttctgcgta
    34261 tttttctaaa ctttttttgt aaacatttag aaaggttgtt ttatcgatga atacctatga
    34321 aataataaaa gacttggcga agaggaaaaa gatatctata cggcagttag aaattaattt
    34381 tggatattct aacggttatc tagcaaagtg gaaaaataat actcctaatt cagatgaatt
    34441 aagtcggatt gctgactatt tcgatgtctc tgtagactac cttctaggta gagaagaaag
    34501 agaaacccct aaacatgtgg atttatcaga agacgatact gttttttctt ttgatggaaa
    34561 agaaatatct aaggagacaa tgcgtaaagc gattgcaatt gctaaagctt tagaggaaaa
    34621 tgaatagttg gagtgatggt ttgtatgtat ttgaaattga aagaaatgtt gagcgaatac
    34681 aatttgaaag tagtttatat ggagatgaaa gagcctggtt tctactatcc aaaacccaga
    34741 atcatttttt tgaatgaaaa tctatacggt gaaactgcag aagcctttca tttatctcat
    34801 gaacttgcac atttcagcgc ctctcatttt gaattttcag ttttgtacga tacttctaca
    34861 acttttcact caaaatttga aacagaagca gataaaatag ctattctaat tttattaaat
    34921 atctatattg agaacgaact gactgatgaa tctcagttta atctcgaaaa atttatggag
    34981 tattattcta ttcagaataa gctcagatac acttgttatg ctgtctgcca atgctatttt
    35041 aagaaaaaat attcttacgc aaggcaatat gtatgaatac atcaagaatt attaaaaaaa
    35101 ccgatttcag caaaacatta aaaaagcccg tgctgcaaca cgggctcttt cctcatatat
    35161 gagcttctac aaaaaaaatc atatcacaga aatgaggaag aaaaaatgga aaaagagatt
    35221 ttaataaaat ctccaggaaa aaccttgatt aaagtaactg atgattctat ttcaattatt
    35281 agaaaaggat ttataaattt agttaatcaa ggtattaaag gagaaaaaac tatccccttt
    35341 aaaaatatat cagccgtaca attaaaaaaa ccaggtatga gtaatggtta tatacaattt
    35401 accttgctag gcggaaacga aagtcgcgga ggtatattag cggccacgaa agatgaaaac
    35461 accgttatgt ttacaaaaaa atattggaat gaaatggaaa atttaaaaaa atatatagag
    35521 aagcaacaat ctatattaga taataattcc aaagaaacac aaatttcaag tgcagatgaa
    35581 attaaaaagt ataaagagtt gttagatgaa gggattatta atcaagaaga attcgatttt
    35641 aagaaaaaag aattgcttgg tttataatat tcaatatcaa aagagaaaga ggatctatta
    35701 atggacggta tatttggttt tactggtatt gttttattta ttataggttt aatcatgtta
    35761 attgtaagat ttatcaaaaa aacaaataag aaaacaccta tgattctcct tattatcggg
    35821 attattttta ctaccgtagg tttctcgctt tcctcatcaa acgaggcaaa tgacaataac
    35881 aaacaggaaa gctcatcatc tgatactcaa actactacat ctaacagcag caataaaagc
    35941 ctaactcaat ttgaagaatt tttagagaca aataatcatg actggggtgc tttcttagat
    36001 agttattatt ctattactcc agctactgag caaaatacag cttttactaa atatattagt
    36061 ggtaaaacat atacttttga aggaactgtt atcgaatcaa tgactactag aatagccatt
    36121 atagcagaca aagagtacga tgataaaagt tggaatgata tttctgcaac acctaaagtt
    36181 tcatatgtaa tttttgcgaa ggacttaaac aatgctgata cctttacaaa gggcgataaa
    36241 gtaactttta caggagaaat tagttcaaga ggatcgaaca tagaaaaatc ctatgctcaa
    36301 tgggatatga ttaatagtaa agtaagtaaa aaataaatga accacgcccc actaagagtt
    36361 agattgtgca caataataaa acctatacaa aagagtgcca tcaggagtca caatgcttgt
    36421 aaaggaaatg acaaaatagg aggtaactat ggataatagc aatgaaaaac taaccaaaaa
    36481 agaatatctt gaattaatag ttgaaggcgg tattggagca attccttata ttggtggcgt
    36541 tgtgcagtca ctttattttg ggtctaaaaa cgagaagcgt ttcaaacgca tcgagaaatt
    36601 ctacaaaaat ttaaacgaat cattagaaaa cattcgcgat caaattccag aagatgtttt
    36661 caacttagag aacaaggacc aactcattgg aatattcgaa gggataaatg acgaggtaga
    36721 aaaatcaaag gcacaaaata aaatagatat gtataaaaat ctttataaga attgtttact
    36781 tcgaattaac aacgcttcat gggataacga ggagtattat cttcttgtac tgaatcaatt
    36841 aacatcgatt gagattcaat tgttagcatg cttaatgagt cgtggaaacg aaaatttcac
    36901 tggaaatatc agcatgtctg gctattcaca agagctaata gatggtagct taaatagatt
    36961 atctgatttt ggtctgttag aaaaaacgat tagctccata gtattaggtg atgtcggcaa
    37021 acaaaatatg ggctacaaaa tatctcattt aggaattcaa tttattaact caactcttac
    37081 ttaagttttc tttctaatat gacatctaga cctatcagca atgcaacaat aaaaaaccag
    37141 acataccaat aatcaacaaa taatctaaac tctggaagtt cagttattct tttaaaaatc
    37201 actttaatca taataaacac ctctgattgg atgatctaat acacatccaa ttataccatt
    37261 aaaacgacaa atagcacatc cttgcgccgg caagcaaaaa ggatgtgcaa aaaataataa
    37321 aacccaaaca atgggcttct ttatagttcc tattgtatca gagaaagaga gctgattcaa
    37381 ttatggcaag attagtcaaa cgtggaaata gttggcaata cgaaatttca tacaaaaaag
    37441 atgacggaaa atacacgaag ataagaaaat caggatttaa gacaaaagga gaagcaaaag
    37501 atgccgccaa cgaattagaa tataacttga acaaaggcct taaaggggat cgcaaaaatc
    37561 tattattatc agattacttt gaggattgga tgcaacttta taaagaagga acagtatctc
    37621 ctatcactta tagaaaatac gaagatacgt taatgaacat aaagaaatat atgccagcgg
    37681 tattgatttc tgatttagat agagtgggat atcaacgctt tttaaataag tatgctaaag
    37741 accatgtaaa atctaccgtt attaagttta ataaccatat tagagcgtcg ttgaaagatg
    37801 ccgtagaaga aggattaatt ccgtttgatc caactagaaa agcagtaatc aaaggaaaag
    37861 attcattgaa gccaaaagaa gataaatatt tagattatga tcaatttaaa tctttaatga
    37921 aactcgtaga agaaaacctt tctgcgcagt actcttctcc tatgctcgtg ttagttgctg
    37981 gtgccactgg aatgcgattt gctgaacttc taggattaac atgggaagat atcgatttcg
    38041 aagatcaaat catcactatt aataaaacat ggaattataa attaaatgaa tggggaaaaa
    38101 caaaaaacga aacttcaaat aggaaaattt ccattgataa acatacgatt gatctcttaa
    38161 aaaagtttaa aatcaatcaa aaagaattat tcgagaattt tgaaatcaaa aaccctcata
    38221 attttgtttt tttcaactta aaaaatggat tagtttcatc aaacgccgtc agcaaatatt
    38281 tgcgcaaaaa attaaaagaa ttagggattg aaaagcaatt tactttgcat ggactaaggc
    38341 atacacatgc atctatttta ctttatcaag gagtaaatat acttagcgta tcgaaacgtt
    38401 taggacatag cagtttagaa actacaatgt ctacttatct tcatattgtt cgagagcttg
    38461 aagatcagga taaagaaaaa atcaatactg tgttcgatag tttatataaa aatgataact
    38521 agtttggcac atttttggca caaatacaaa acaaacccct gtaccgcaaa ggatacaggg
    38581 gttatttata cgttcctgac tggaattgaa ccagcgacct atcgcttagg aggcgattgc
    38641 tctatcctac tgagctacag gaacaatttc aataaatcaa ttgtagcttg tttgtttttt
    38701 acttgcaagc ttatctgcta aaaaaatcag cttataagtt aggcaaaacg tttccactag
    38761 tccttaacag atgataaact agaggtagaa tgaaataatg tagggaggtc ctttccaatg
    38821 aatgataagc caaaagaaaa agtatacgca gaacgcttag attcatctaa aaaaaatcgg
    38881 gaaaagatta cgaaagaagc aatccaagac cacaaaaaag aaaaccctaa aacaaaagaa
    38941 aacaaaaaat tcaaatttac gaaaaaagag gatttacttt aacattttcc ttcaaatgcc
    39001 aaatgaaaaa caaaagggtg gtgacaaaaa t
//
LOCUS       DACEPD010000015        37959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.015, whole genome shotgun sequence.
ACCESSION   DACEPD010000015 DACEPD010000000
VERSION     DACEPD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.015"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(175..1485)
                     /locus_tag="IW797_000802"
     CDS             complement(175..1485)
                     /locus_tag="IW797_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1223594.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1497..2915)
                     /locus_tag="IW797_000803"
     CDS             complement(1497..2915)
                     /locus_tag="IW797_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1223595.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3155..4723)
                     /locus_tag="IW797_000804"
     CDS             complement(3155..4723)
                     /locus_tag="IW797_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ1223596.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4727..5137)
                     /locus_tag="IW797_000805"
     CDS             complement(4727..5137)
                     /locus_tag="IW797_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ1223597.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5134..6057)
                     /locus_tag="IW797_000806"
     CDS             complement(5134..6057)
                     /locus_tag="IW797_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ1223598.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6054..6932)
                     /locus_tag="IW797_000807"
     CDS             complement(6054..6932)
                     /locus_tag="IW797_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1223599.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6919..8256)
                     /locus_tag="IW797_000808"
     CDS             complement(6919..8256)
                     /locus_tag="IW797_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ1223600.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8259..9362)
                     /locus_tag="IW797_000809"
     CDS             complement(8259..9362)
                     /locus_tag="IW797_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1223601.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9436..10944)
                     /locus_tag="IW797_000810"
     CDS             complement(9436..10944)
                     /locus_tag="IW797_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1223602.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10910..11602)
                     /locus_tag="IW797_000811"
     CDS             complement(10910..11602)
                     /locus_tag="IW797_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1223603.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11624..12598)
                     /locus_tag="IW797_000812"
     CDS             complement(11624..12598)
                     /locus_tag="IW797_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1223604.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12898..13770
                     /locus_tag="IW797_000813"
     CDS             12898..13770
                     /locus_tag="IW797_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1223605.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14228..16426)
                     /locus_tag="IW797_000814"
     CDS             complement(14228..16426)
                     /locus_tag="IW797_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1223606.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16658..17650
                     /locus_tag="IW797_000815"
     CDS             16658..17650
                     /locus_tag="IW797_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1223607.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17710..19131
                     /locus_tag="IW797_000816"
     CDS             17710..19131
                     /locus_tag="IW797_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ1223608.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19124..20623
                     /locus_tag="IW797_000817"
     CDS             19124..20623
                     /locus_tag="IW797_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ1223609.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20662..22056
                     /gene="uxaC"
                     /locus_tag="IW797_000818"
     CDS             20662..22056
                     /gene="uxaC"
                     /locus_tag="IW797_000818"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ1223610.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22215..22898
                     /locus_tag="IW797_000819"
     CDS             22215..22898
                     /locus_tag="IW797_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ1223611.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22900..23736
                     /locus_tag="IW797_000820"
     CDS             22900..23736
                     /locus_tag="IW797_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1223612.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23747..25501
                     /locus_tag="IW797_000821"
     CDS             23747..25501
                     /locus_tag="IW797_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1223613.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25777..26118)
                     /locus_tag="IW797_000822"
     CDS             complement(25777..26118)
                     /locus_tag="IW797_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1223614.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26186..28357)
                     /locus_tag="IW797_000823"
     CDS             complement(26186..28357)
                     /locus_tag="IW797_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ1223615.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28568..29425
                     /locus_tag="IW797_000824"
     CDS             28568..29425
                     /locus_tag="IW797_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ1223616.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29496..30353)
                     /locus_tag="IW797_000825"
     CDS             complement(29496..30353)
                     /locus_tag="IW797_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1223617.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30338..33040)
                     /locus_tag="IW797_000826"
     CDS             complement(30338..33040)
                     /locus_tag="IW797_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1223618.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33053..34342)
                     /locus_tag="IW797_000827"
     CDS             complement(33053..34342)
                     /locus_tag="IW797_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ1223619.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34492..35538
                     /locus_tag="IW797_000828"
     CDS             34492..35538
                     /locus_tag="IW797_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1223620.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35540..37693
                     /locus_tag="IW797_000829"
     CDS             35540..37693
                     /locus_tag="IW797_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ1223621.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11213 a         7812 c         6760 g        12174 t
ORIGIN      
        1 caagtggttc tttttaacga cttagttatc ttatcacgtc taaaaacatg tgtcaactct
       61 tttttggaat tttcataaaa tgtttcaatg aagcgcgaca acgttgttta atatatcatt
      121 aaaaaaaata aaggtcaagc taaaatatta tttaagcttg accttattat aatttcaacg
      181 attgtcaaaa gtattgtttg cagctgtgtt gccagtttct atagataaat cacagctttt
      241 gaaacataag ttttgtactg cttcgattcg cccgaattct tttgcagtta tttggcaatg
      301 agaaaagcag acatcttcta taggcttttc aggaaatgct actaaatcaa aagctcggga
      361 aggaagtgag taacctggag aaaggcatgc ctgtacgttt ttgatgtgta tgtctttgac
      421 tttcgtcatt tgctttgatt caggtatttc ttcagccact gcttgccaac tttctggcat
      481 ttgagcaagg tcattgctat tttttctatt gtattcatta tgccagtcca ttatccaaga
      541 aaaaggaaat tgtacatcaa tcatattcag attttctacc ctgatatttt cgatcactcc
      601 ccctctttct tttgaagatt tcattcgaat accacagtct gactgaaaaa aatcgatatc
      661 gtgaatatat acgtcactaa ctccagcact aacttcgcta ccaatagtta caccgtatcc
      721 tgaacggatt ttacatctag caacctcgat tcttgatgat tttcgattga ctcttgcacc
      781 attgccatct ctgccagact taatagcaat acaatcatca ccacaggcca aatcacattc
      841 atagacacgc acatttgttg aagaatcaat atcaatccca tctgtacttg gtccatcatt
      901 gttgcggatg attacttttt ctactaatac atcgttagaa taagtgattt gcaagttcca
      961 aaaacctgat ttctgaaaaa tcaaatcacg aacaacaaca tgttctgcat gatacagtaa
     1021 acaagcgcgt ggacgtttaa tagcataatc cgcaatccaa cgaagtccct tttgatcgta
     1081 ttcagctctt gtcccgcttt tttgatcttt tccccaatac agctcccacc aatggtctcc
     1141 ttgaccgtcg attattcctt ttccttctat aagaatatct ttcgccgaga gaacattcaa
     1201 aatcgctgct ggccatttca tttctacgcc ggctactcgt gtatcgattt ctggaaatgc
     1261 ttcgatatct gttctcccct tgaggactgc accttcttca aagataagat gactcttatc
     1321 ttttaaaaaa agggctccga ctaaataaat gccttttgga ataaatacag gtaagtgttc
     1381 tttcgcacct tgatcgattg ctttttgcag tattattgta tcatcttgtt gagtgtcgtt
     1441 tgttttaaag ttcaaagcat tgataatccc tttttttgtc cgcaatacgt ttctccctat
     1501 ctttcattga tttttttcaa ttcttcacca ataaatacag tttttgaacg attcagtggg
     1561 taacctacta gcaaaatgat tgcggataca aataagcaga atgctggtaa caagtttgcc
     1621 atcgtataaa tcctttcttg tactaaaact gattggatgg ctccacctgt tgtagacgat
     1681 tggtaaccaa tgagtgccag cataaatccg cctaccgctc ctgcacatgc ttgtcctact
     1741 tttctcgcaa aagaatttac tccatatact gttccatctt ctcttaagcc tgtcttatat
     1801 tgatgataat caatcacgtc agtaatgaat gcccagacca tcaaattgaa taaaccagat
     1861 cctaaagtag cgataaataa aagaatcaga tatacccatg catttgttat atgaaggaaa
     1921 tacatcagca agtacataca tgaagaagca agtaacgcaa atacgcttgc ttccttttta
     1981 ccaaataatt ttgtcagctt tgtagcaaat ggtgctaaaa ctactactgt tccgtaagta
     2041 aataaaagag caacagacat tgcttgttta ttatggaagt aatcattgaa taaataagtc
     2101 atattcgtcc cagctaaaat ctgattgatc acaatgaaaa tgtcaacaat gactaaaata
     2161 atcagtgctc gattagctaa caaacctttt accaactgag aagcagatac tttttcgttt
     2221 ttgacaactc ttactctttc agtagtcaat ttacaagtta acatatatgc gacaaatgct
     2281 attgcagcac atccgattga aatccaaaag aaatgattcc ctgaaacgac ttgttgtcca
     2341 ctcttgttat gggtatacat taaaatcgga atgaagaatc ctgtagttga tccgccaatc
     2401 gcagaaccaa tgcttcggaa cgtggacaac gaggtgcggt cattaggatt tgagctaatt
     2461 gctgctgcca ttgctccata cgggatattg attgttgaga gaaaaatccc ataaacaatg
     2521 tatgaaacaa aaatataagc aatcttcacc gtcattggaa gatgttggac gaatggcaaa
     2581 aacaaaatga cagtcactaa gcaaaatgga tacttcattt ttctgatcca aggaataaat
     2641 cggccagttt ctgttaactc tgagacatca cataatcttc caaccatcac atcagcaaac
     2701 gcatccaaaa ttctcgaaat caagaataac gtacctacga ttgcccctga aacgccaaga
     2761 acattcgtat aataaatcat caagaaacta ttgactaaac ctaaaataaa acagttacct
     2821 agatcaccga acatgtagcc tatcttatcg gaaatcccaa attccttttt ttcttttttt
     2881 attgtatttg ctgtaacagc attttgtgaa agcatttgaa ttcctcctac ctcttctagt
     2941 aaacgttttc aaaattataa ctcttttttt cttgttttta tattcacaat gttggctttt
     3001 tattgcatta tcttgacaga taaaaaacaa aaaataatct aaaaacattg atgcataaga
     3061 ttgataactt tttttacata acaatatata caaacaaaaa aattcactaa ctagtgaaaa
     3121 taagttagtg aacctcattt attaaagaat taaattacag atgatctttt ccatcatact
     3181 tgaaaaactc aaaatcagca taacttcctc tttttttatt tagatcatgc actgctatcc
     3241 cgataaaatt tccagtaaac cctcctgaga gtatacacat gtttttttgc ggacctactt
     3301 tcttccattc ttcagttgat acgtctttca aatgaaactg tgctgtctct ttctttacag
     3361 tgactttcaa aaaagtcttc ttttctgtga gtggaatcgt ccaatcttct atttgtgcta
     3421 ctccgttttc tgattctatc atacgtaggc attttccctt gtcctcattc caagtaacat
     3481 aacaataaat ataattggat tcatttaagt acagtaccag tccagccatc tgattaaaat
     3541 tattaggcat gtattcaaat gctgtctccg catcaaaagt aaaatccgtt tgccgaatag
     3601 ctaacaaatg atgttcgaag gtagattgaa gagattcacc ggaaatcaga cgtaaaaaac
     3661 ctggtctaca ttttgtatca caccaatcag catttggcaa aatgcgtctg gcattccagt
     3721 tcttctttag ctctccttca aatgtatcca taaactgatg atctttttct tgttgtacct
     3781 cttcatttgt ctcaatcatt atttcttcca tcggagttgt ctggctatct ttaagtctta
     3841 accaatcgtc ggacgtccaa tacacttctt gaatagcggt ttctcttccc aaaatagccg
     3901 caccgtttaa tggtctggta caaagatatg tcatgtacca tcttcctgac ggggtttgta
     3961 ctaagctccc atgtcctgta cattgcaatg tagaagcagg tttgtcacta gctgtcagca
     4021 taggaaaacc aggatcaagc tcgtaaggac cagtaatatt ctttgctcgg caaactgtaa
     4081 ctgaatgtcc tcttcctgta ccgccttctg ccgtcatcaa ataataatac ccgttacgaa
     4141 aatacaaatg tggtgcctcc gttttcgcta attctgtacc tgcaaatatt ttatagatgg
     4201 gaccatcaag cttcttcttc tctgcatcat attcttgtaa tacgataccg ctggatttat
     4261 tcggtgtgtc cagacgataa tcccatagtt cattgacaag ccattttttt cccgtttcaa
     4321 gatcatggaa caaagaagga tcaaagccgt tactatttaa ataaattggt tcggaccaag
     4381 gacctgtgat ctcagatgca gtgatcaaat aattgtggac atctttaaac ggacggcctg
     4441 cacttttgac atctgtgtaa acaagataaa acaatccatc agcataactg atctgtggcg
     4501 cccatatact tccatttgta ggatttcctt gcaaagatac ttgagtggtc aaaatatccg
     4561 ttgtatgtga ccagttgact aaatttttcg actcatatac tcggattcct ggaagccatt
     4621 caaaagagga gacaataatg taataccgct ctttcactct taatatatta ggatctggat
     4681 tgaatcctct taaaatagga tttttcactt tgatagtctg catgattcag gctccttttc
     4741 tcattcttac tcgatataaa ctgaatgccc cagctgcgaa ataaaaaaat gcaaaaggaa
     4801 acgcaataaa ctgagtgcca atcaacaata tgaatcctgt tataaaatac aaaccagtgg
     4861 cagttgttaa gtattttcct ttagcagtca aaaacaatgc tactgctaaa aaagcaagtg
     4921 aaataaacaa cacaacggca aacagaccag tcatgtcctg taataatcca aatgtatttt
     4981 ctaaagacaa agttgaatga tctacagcaa gcgactgata acttttttta aactctgacg
     5041 cagacatatt acttaaagta actgctaacc cgccaaagaa aatcaaagat agactgccac
     5101 cgaccattcc tagcaatagt tcccattttc ttttcatttc tgaaccccct gcttttctaa
     5161 atgtatccat tcaggtcttt tgatcgatgt ttcataattg ttattacgtt cagagaattt
     5221 gacagtctgt tcatttttct tgtcatccca attatcccaa ccagttgggt gaacatgccc
     5281 acctatctta cacgcttcaa ataagacatt tgcatacgga cgccacggtc ttcctaaata
     5341 gacgtttcta gtatcaggtt ctgcagtcaa ggtacagctt tcaaaacgat accctgtttt
     5401 ttgatttttc ggcgtagatg ctgctgtgat gtatccagga tttttactgg ctaagctatg
     5461 gatatgacac tgatgaaaaa cggcatctgc cccaccaaat atgaaatcca ccgttccttc
     5521 aatccagcaa tattcatagg tctgacgaca atactcagga ttggcagaag ccggtgtaca
     5581 aaaaggagta ccatcttttt gcaaatcagg caacggaccc gtgcataagg tatcctgata
     5641 accagatagt cgacagtttt ttaaagatac gcgatctcct gaattgaata aagcaacagc
     5701 ctgtcctacc ttttctcctg gacctgctgt attttcgatg tgaagatttt ctaatacgac
     5761 attcgatcct tctaagaaaa aagtagcact tcggaaagtc cctaactggc taccgtcaag
     5821 atactcctgt tttgcaaaac gattaccgga gataactact gttcctgctc ccacaagttt
     5881 aaaattggat aatctgcttt ctatatattc atcatatctt ccttctttta ctatcaagac
     5941 ctttggatgg ctatccggca ggcctgccaa ataatcgatt ccttcttgga tagtttcaaa
     6001 atccgtcggg gtatctatag cattctttcc aattatcagt ttgtctttca tcctcataaa
     6061 tgctccctta tgaattgttt ccaaatattt ctcatttctt gtgtttccat atgaccgcct
     6121 gttaaagaaa aagaagaaaa gttttctgga ctacccattg attgatactt tttgtccagc
     6181 ccttttctca gcttcagtac gccttcgtcc aagcacatcg tatcgttttt tccaactaag
     6241 ctaagacgag ggcgcggagc gattttttct tggattgcta acgttgtata agctgtcaat
     6301 agatttggca catagtaata aaacccatga tgatctaggc ggcgatgtag caaaagtatt
     6361 tcgatgtcca cctgtgccgc tagatcaata caaactttga cacgagaatc caatgctgtc
     6421 acccaccagc ttaataagcc tcccatagaa agaccaatcg ttgcgagccg ttgagtatct
     6481 gtgtcttccc ttgtctccaa ataatcgatc aagctgatca catcataaat acgcattccc
     6541 cataacgttt ttcctgttaa taaaaattgt ttgaacaact cgctttcaga aatacctccg
     6601 cgttcttcaa atccccaagc atcaatcgac caaacagcat atcccattcc tgtcagtact
     6661 tctgcaaaag atggagaagc taagtaagga gcaccatgga tcaattcact tttcccgact
     6721 gaaaaatctc cgccgtgaga atgcaggtaa aggaccggcg gaaacggtcc ttctttttct
     6781 ggataagcat agagggctgg tactgtttcc aattcattta agtacaattg atagtattca
     6841 agcataaaac cgttttgttt ttctcggtat aatcgttttc cactaggggt atgtctgcta
     6901 gatggagcac ctaaaaaatc attcaactgc ataagcaaag tttttacgaa caaccagttc
     6961 atccatattt tctgattgat ttgattggaa tttctcaata ctgatttctt tagtattttc
     7021 taatagaaat gctggtccct cgtgattttc aatcgacatc tggttaaagg aaaccttacc
     7081 cgcaaagcca atataaaatc cttgtctgct catttcctct attcctgtca tcatagcagg
     7141 aacaccggga actgcatctt gggccatcga gacatcgata ttgtcaaatg tgatttcttg
     7201 tatgaattgt tccgctaatc cataaataaa acctgcagaa gcatggacat tccttgccgt
     7261 gatattcgaa aaatggatcc ttctgaatgc tggtgtccgt tcatctatcg gataagcctt
     7321 tttttcccat acatacggtt ctttccctcg cggaccacag aaataataaa gattcagtat
     7381 aaatggacac atcacattat ccatgactat attggatact cgaatatctt caacgatccc
     7441 gcctcgacct cgtctagatt tcaatcgtat tcctcgatca gtttcttgga agatacaatt
     7501 tgaaatggta atattacgaa tactaccact catttcactt cctaatacga caccgccatg
     7561 tccatggacc atcgtgcaat ttgtgatcgt gatattttcg caagcgatcc tttcgtacgt
     7621 atcttcagtc cctgccttga ttgcaataca gtcatctccc acgtcaatat ggcaattact
     7681 aatacgcaca tttttgcatg attctggatc aattccgtct gtatttggtg aatccgctgg
     7741 gttcaagatc gtcaagttat caaacgttgc attactgcaa agaatcgggt tgatcgtcca
     7801 acttggtgac tggatcaatt taatatcttt cacagtaatt cgatgacagt tatggaaact
     7861 catcaattta ggacgaggat aagcgagatt atctggttca tttctaaaag tatgccacca
     7921 tttttttcca tttccatcta atgtaccgaa accagtgaca gagatgttct ctacattttg
     7981 agcatagata caggaagcat aaacttttcg atggactcct tcccaacgtg aatgaacaac
     8041 aggataatct tttggatcgt cagagaattt cagaaccgct cctgcgctta aatgaagttc
     8101 gacattgctt tttaaaaata aagctcctgt tagaaattca cctgctggaa caactacggt
     8161 cccgcctccg tcacttgctg cagcatcaat ggcttgttgt attgcttcag tattcaattc
     8221 atcaattgat gcgccaaatg tcaaaatatc atacatattt ataatgcctc cacttcaatc
     8281 aatgcttgca agaatgcacc aacgcctttt tgatcattac aaataattgg ttcactgatg
     8341 tagtaagcat atgaaccatc tcgctgatct gctccgccta accctgctac ttgacagttt
     8401 ttattcaagt tcagccagcc ttcttttgtt tccagaacaa attcatcaag taatccttga
     8461 tgtgatttct tcacttgtgg aagccattct tctttgttca gcagtcccaa acgtattcct
     8521 ttagccattg cgtaaacaaa catactactg ccggaagctt ccagataatt tcctttccgt
     8581 tttgtttctg ttgttacttg ataccatgtc ccgctgtttt cttcttgata tggaagtaat
     8641 gcagccagtg tcttttctag aatttctttc agcaaagaac aatctgtttc ttcatgaagt
     8701 aattcatagg tatctgctgc agccatcaaa taccagccaa tcgatcggct ccaaaaatga
     8761 tgggatagac ctgtttttgg atcagcccat ggttggacct tcttttcatc ccatgcatga
     8821 atcagcaaat ttgtttttgg atcgaataga tgtttataac tgatttcaaa ctgtctcaaa
     8881 acatcttggt aatctttttc ttcactaaaa ttctggataa attcagcata aaaaggagaa
     8941 cccatatata agccatccaa ccaaatttga taaggataaa tttctttatg ccaaaaagct
     9001 ccttcggatg tgcgtggatg gtttgccaac tgctgggaaa ggagttctgc tgctttttta
     9061 tatttttctt cgcctgtttc tttgtacaat aaaaagaaaa gctttcctgt atttacatgg
     9121 tcgatattgt attcttcaac gctgtagcca cggatcgttc catcttcttg cacgaaatag
     9181 tcgatattgt cttttatgaa atcaaaataa attttttctt gggttcgttc ccaaagtaat
     9241 tggaaccctt taagtacgac gccatagtca taagaccatt ttcctttata ccacttttct
     9301 tcatataagc gaggtgttct ttccatgata ctttttgcta atttctttga ccagttttcc
     9361 attatttgct ctcctttaaa aattaaaata ggatttggga caaataccaa gaagtatttg
     9421 tcccaatcgt ctatattact gtgccgctgc tttgtatgct tcctcgtact cttgtgtgat
     9481 tcgatcgcct ccttgtgctt tccagtcttc tacagctttt ttgaatccat cttcgtcaag
     9541 ctgtcccatg atgtattgca ccgtagcatc aatgacgatt ttttcaattt ctgatcctac
     9601 ttcattgttc gtcggtgaat ccaatggcac agaaggatcc attacagcaa attcttcatt
     9661 ttctttgatc aattgattag agaattcttt ttcagggtct gtatctttga aagagaacgt
     9721 cacttcacta ggacgagagc ttgataacgg ttgcacctct tgttgccaaa gatccatatc
     9781 gataaattcg atagaaccat catcattcaa ttcataatgc gttccttcga taccgccagt
     9841 catcaattta tacacatctt cttctaaaag atcatcaaca aattgaagta atttcttcaa
     9901 ttgtgcttcg tcttttactt gagatttggg aaatgctagc aagccgccaa taccatttcc
     9961 ttctgaccaa acatgataac cgccgccttc accatctgag atcttgttta caggcactaa
    10021 ttccatatca tcttgaatac cttgacccat cgtcttcaga ttcttgatat ccaccattcc
    10081 agtgtagatt ccagttcttc cttgtgcaaa ttgttcttgc tgatcagtct tttgggtaac
    10141 tgcaaagtct tgtgccaaat agccattctc aaatagatct cttgaaaaat tcaaagcttt
    10201 catatactct tctgtatcaa attctggagt gaatttgcca ttatcgtcta cagcccagcc
    10261 atttggtgct ccaaaataag aagtgaaaag tttaaagctg gtataacgaa tatcactagg
    10321 cgcacgatcg ccaaaaccaa ctgtgtcgtc cacaccattt ccatcaggat cgttttctgt
    10381 aaattgttta gctacttcat aaagctcatc catggtagtt ggaacatcta gtcctagatt
    10441 atccaaccag tcttttcgaa tgactaagcc agcacgagcg tagtcttttt gtatcggtac
    10501 gccgtacaag gcaccgtcga tactcgcagc ttcgattcta tcttcagaaa tttgacttaa
    10561 atttttatag tcttttaaat attctgatac atcccagaat acgcctgatt ttaacgcatt
    10621 gcggacagaa gagtttttca acatcgcaat ttgaagtgta acgatatctc ctagttcatc
    10681 agaagccaat gcagttgtta atctttcttc ttttgaagca tcaggaatcc agttgaatgt
    10741 cagttccgta tcggtttttt cttcgatcaa gttcacaatc gtgtctgtag gcggtgaagc
    10801 atgatgcaaa atatttaacc aagtgatttt agcttttcca tcttctgtct ctgaagaact
    10861 gttacttgaa ctgctgccgc atgcggcaag tgctagaatt cctgcaaaac taagacctaa
    10921 ttttacgagc gttgcttttt tcataaattt tctccttcac tgttttaata atatttttgt
    10981 tttcttctgt tttttctgta tatgagttct tccattccaa atggcaaaat atagaatttg
    11041 ctgtccacgt gactgcggaa aagaagagac tgccaccgat aaaggttaaa acacctggca
    11101 ttgtttggat gatcatcaga taaatcccta ccatacttaa tatcaacact aatgagtaat
    11161 gaagatgtga gaatccgaat aaaaatgcca ttttcatttt gaaaaaatat cctttccaat
    11221 cataatgagc catgattggg aaaatataga gtgctgagag cacatatatg aacgcagcaa
    11281 tatacaaagc gatcctaaga taccaattgt gtatgaaaga aagatccaca agtaaaacaa
    11341 tcccgatggc gccatatatc cagctcatca gcaaggtttc acgatagttt tctttaaaat
    11401 atgtgaagta cgttttcgtc aaaggaaacg tttcttttga gcgcagccat tttctaatca
    11461 ctgcaaccat cgcatacgat gcaggaccga aagtaaaaac tcccaaacct gcgatgacta
    11521 aaccgcccca cagaaaattc aaataaacca actgtaaaat acgatttagc cttccgttta
    11581 ttttatctaa tgactgcagc atatctttcc ctctctttct atcttagtaa acgccgtctt
    11641 ctcctagctt tttggcaatc ttattagcta aaattaccat aaacaatcct acgacccctt
    11701 taaacaatcc cattgctgta ctgaaactta actgtccatt cttcaatcca gctgtataaa
    11761 tataggtgtc gaaaatttct gcaacgcttc gattcaatga attcaataac aagtacatat
    11821 gctcgaaccc taagtctaat gtctgaccga tttttagaat cagcaaggta atgattactg
    11881 gacgaattgc cggcaatgta acatgccatg ttttgcgtaa gcgtcctgct ccatccattt
    11941 ctgcagcttc atacaactgt gtatcgactg cagttatcgc tgaaaggtag atgatcgtag
    12001 accagcccaa ctctttccag atcacttgtc cgatatatac tgtgcgtgtc cattcagggg
    12061 atgttagaaa actgattttt tcaaatccca ttgttgcaag gaattcattc aatacccctc
    12121 cgtcaacatt caaaaataca taagtaatcg aaacaatgat tacccaagac atgaagtgag
    12181 ggatgtaaat gatcgtttga atcccgtttt tcaatcgttt gttcgttact tcatttaata
    12241 gtaaagacaa aatgattggc atcggaaaaa acacgaagat atttaatccg aacaagatca
    12301 acgtatttct taataatgta aagaatgtcg gttctgtaaa caaacgaatg aaatgtttga
    12361 aaccgaccca aggacttccc attattccta aaaatggttg ataatcttga aaagcaataa
    12421 cgagtccccc cataggaaga tacctaaaaa tcaagaaata aaggactccc ggcagaatca
    12481 tcacatataa aaatttattc gttttgattc ctgcaattat tttttttcgt cttctgagct
    12541 tttcttcttt tttcgatagc gtcagttgaa atggttcttg tttctttaca ctttccataa
    12601 gcccctcctt agttttgtga atgtttgctg cttatgactt catgatagat aactatctga
    12661 attacgtata taatcatttt taccgtaagt aaaaacgctt acatagcaat gatttttgtg
    12721 ctatacaaaa cgataaagtg tattttttca caaataaact acgcatagca aaccttctat
    12781 ttttttctat ctgacactta gcataggaaa tgattatata cgtaatcttt caccgatgat
    12841 aaattgaaca tatagaaaca gaaacattta atttattttg aggaggagcc tatctatatg
    12901 aaaaagaaag tctctatagg agaaagaagt tttacacttt tcaacaatac gtttctaatt
    12961 ttgctagctt tgatctgtat cgttcctttt ttaaatatca tcgctacatc ttttgcttct
    13021 acacaagaag tcgtcgcgaa aaagtttatc ctttttccta ctaccttttc tttagacgcg
    13081 tatcgctaca ttctttctac gccgaccatt tttagagcac ttgctgtttc gattggtgtt
    13141 acaggtgtcg gaacgatcgt cagtatgtgt gcaacttcac tgatggccta cggtctttcc
    13201 agaaagtatt tgtttggtcg aggtttcgtc aactttcttg ttgtattttc tatgttgttc
    13261 agcgggggga tgattccaac gttcttagtt gttcgttctc tagggctggt caattcttac
    13321 tgggccatga tcctgcctgt ggctgttaat gcaatgaata tgatcattat gagaaacttt
    13381 ttccaagcct tgccggatag tttggaagaa tcagcaaaaa tggatggttg cacagatttt
    13441 ggtgtatttt tcaaaattat gctgccatta gcgttaccgt ctattgctac tatctcgctt
    13501 ttttatgctg ttacttactg gaacacttat atgactgcta ttttgtatat caacgattca
    13561 tctaaatggc cgatacagat tcttttacgc caaatcgtca ttgtatcaag tgggatgcaa
    13621 gcagaaagta gtgccgttga tgtgattcca ccagcacaaa cagttaaaat ggctgttatc
    13681 gttattgcaa cagtgcctat gctgattgtt tatccttttg ttcagaaata ttttgtcaaa
    13741 ggagcactcg taggttctgt caaagggtga ttatgtactg aacgcaaaat ggtagttgtg
    13801 tcagaagtgg tcagcttcaa gaaataagaa ggcatttacg aaaattgctt cttaaatttt
    13861 tgtgaaattt cggcttattt ttcgaagaat ctactcctgg aacaccgtgt atcagaggtt
    13921 gtgaaagaga cgttcagcct tgagcgatga taaaaactct cacaaacatt gcgacgagta
    13981 acccacagga gcaataagaa gcgattttga aaaatagctt cccatatttc acaaaattat
    14041 gccttattgt cgatagcctg tctcttgaac gccatttatt cgtaaaaaag aactgtgaca
    14101 agacttttgt cacagttctt tttgcgtgct tagccttgaa atttaactat ccataaatac
    14161 aaatactagg gttattctaa cttcttttat tgcttgaatc agtccacaag aaaaacagcc
    14221 tcttgattca caaatatttt tttcgatatt ctcccggcgt aatcgattcc ttctttttaa
    14281 agaaacgaat aaaattttgc ggatttcgat attgcaatct ttctgcaatc tcttttacgg
    14341 agaggttagt ctctttcaac catttttttg ctacctctaa acgatgattt tgaacaaaat
    14401 caccaaattt ttctcctgtt tcctttttaa atacattact taaatagtta gggttataat
    14461 gcaacctatc agcgatcaca tctaaagaca gatcacgatc atattctgtg tagacgatat
    14521 gcataatatt ctccgacaaa tttttaaatt catgatcagt gattgcttga gagcattctg
    14581 tgattggcag aactaattgt tctaaaacaa gtttttctag ttctttggga tgataattgt
    14641 tcagtgcttt tacataaatt tgtttcatgt tatcaaagtt ttttgaatct actcccaata
    14701 actgtcctaa ttgaacgagt tcattgatca agcggaccga aactacttcc cgactcaaag
    14761 gatttttatt ttgtgcaaac aatgcatcaa tcaattcatg tactaaattc ttcacttcct
    14821 gatttccaga acgaatggct tcaaacaatc gattttgttc ttctatcgga tatctaataa
    14881 gtgttttttc atgagcagca ggaacgattt cctgataaaa aataattgaa cttggaccag
    14941 tatttacacg ataatacagg gcttcttttg cccgatcaac cgattgcttg ctttcattca
    15001 gcgtttcgaa ccggttactg atgcctatac tgatagttaa attcaagtat tcttttacac
    15061 gtttttgaat gagtcggcaa tactccatga cttttttgct tgcttctgca tcatctttat
    15121 ccattcgata aatagtggct tgcatttctt cattcaaaac gatcggcagc attcgatttt
    15181 cctttggcac aatttccgtg atcatgttgt tgatcgctaa aagtagcaaa tcacgctccc
    15241 caccgtgact atcttctaac agatcgattt gaaccaaagc tgtataatag cagtcatttt
    15301 gagaactata accaaattgc tgcaaccgct gatctagttc tcgcctttct actcgatttc
    15361 taaataaact aagtacaaat agcgtttcca gctgtggctt ttgggtaact aagtgtgcac
    15421 ttaacgcttc tttttctcct atgatccgac tgactgattg agcgaccaaa tctagttctt
    15481 tcccactgaa tccttttgat ttaagattca gacgttcctg tatttgagaa atcggacggg
    15541 caaatctgtc cgaaaataag taggaaagaa gaccaatgag tcctaccagt gttaaagaca
    15601 ctgcaactag cccgattctc agattgcgga tcattgcgcc aattgcacta gagtcaactt
    15661 ctaatctgta aatccaatta ttataatctg atttcagtac tatatacgtt ttatcttttt
    15721 ctcttatcac tttcagctga ttgtaatcta gatccacgtc cgttaataca gttggttgct
    15781 ctttattttt tactgtctga gtagtaaaaa ggttgccaac ttcgttttca atagaaagta
    15841 tgtcttctcc actttcacca acaacttgtt ctattgtatg tttctctatc gtagcaattc
    15901 ccaatgcata tttttctttt tcaaacattg gcaaagaaat caccatttcg attcctttgt
    15961 ttttcttttg ccaaaataaa ttatttttgt tatttatgta cttctgttga tacttttcaa
    16021 tttcctcttc ggtcagttga ctcaacgatc cattgattac tccccattta ccagccaaac
    16081 tgactaaatc ataattatta tcatccataa caatcgtttc tataaaattt agttctttcc
    16141 tgatttcaga ataagtggag taatctgtat atgtcagcgg atgcttgaat atgtccgaga
    16201 aatttttggt cgaaccatac gtagaaaacg aatattcaat cgttctaatt ttccattcaa
    16261 cgttagtttt aacttgcctg agcaaattct ttttgtctgt taaataactc gattcgatcg
    16321 attcataggt attctggtaa atgaataaac taaatcctat gacaagtacg gctcctaata
    16381 aaaacatcgg taaaaatatt ttataaaata ctgtactttt cttcattttc ttcctctcct
    16441 ccttttaaaa taaaaggctt tcatttttct gtaaaattga tactcatcta attaaatgag
    16501 cattccccgc tttcaagaat agcataatta aaaataaaaa tccaagtaat ttttaacgtg
    16561 tgcaatcttt tcatatcaaa gatctattga cttatcgtag ttatcgtaat atgatgaatg
    16621 cgttaacaaa aagtgaaaaa gtaaggaaaa tgatggaatg gtaacgatca aagatgtcgc
    16681 taaagcagct ggcgtttctc atacgacagt ttctcgtgct ttgaataaca gcagtttaat
    16741 aaaaaatgaa acaaaacaaa aaatacgaca actagctaag gaaatgaatt attcgccaaa
    16801 ctatagtgca aaaggtttag tgaatcaaaa aaaatatcta atcggactat ttttctcaag
    16861 tttgcataaa ggaacttcat caaatttcct agcagattcc attgcaggca tccattcagt
    16921 attagacaca aattacagtt tatctgttga aagtatcgat gttgttgatt taacagaaat
    16981 caacctgcaa cgctacgatg gactgatcgt tatgagtcaa agtgatgatg atcagccttt
    17041 catcgactat ctcaaagaaa aagaatttcc tttcgttgtt gtcaatcgtc acatcaatga
    17101 tccagcggtt atcaacgtca ctgccgatga tgcggccggt gttatgcagg cagtagatta
    17161 cggtattgga tgcggacata cgaagatcgc ttatattggc ggaaagaaaa attttcgttc
    17221 gacaaaagaa cgggaaaaag gggtattgaa aagtctaaaa aaagcgagca tccctgtcaa
    17281 tccagcctat tttttaacag gagattacag cttagaaagc ggcctccaaa acatggaata
    17341 cctattatca ctttctgacc tgcctactct ggttttttgc ggcaacgacg acatagcgat
    17401 tggtgcattg agagctgtac atttagctgg cctccgtgtt ccccagaaca tttctctgat
    17461 cgggttcgat gattctccag tcgtctccta tctcaatccg ccgcttacca ccgtacgtaa
    17521 gcctttgaaa gaaatgtgtc aagaaggaac aaagctttta ctagatttga tcgaaggcag
    17581 gacgatcgaa caatctaaaa ttgagctctc caccttgttg atccttcgtg agtcgatcaa
    17641 ttacttgtaa actacaagta attttatata aaaatgttaa cgtgtgcata acacagaaag
    17701 gaagtttata tgaaaaaact gacaaaagaa ttagtgaaaa aaaacgaagc acctgtaaaa
    17761 gttcttcagt ttggtgaagg caattttatg cgtggattta ttgattggca gattcagcag
    17821 ctgaataacc aacaattgtt taaaggtaat gttgcaatca ttcaaccttt agaacaaggt
    17881 cttggaaata tgatgaacca gcaagataat ttatatacgg tcattttaca aggactcagc
    17941 aatggcgaaa tcgttgatac ttccgagatt atcaccgtag tagaacaaat gatcaatcct
    18001 tatgatgaat gggatgctta tctatctcta gcagaaaatg acgacttggc ctatatcgtc
    18061 tccaatacaa cagaggcagg aatccaattc gtggagactg ataatttaac agatactccc
    18121 ccacagagcc tcccgggcaa actgacagcc tttttatacc gacgtttcaa attaaacaaa
    18181 gctggcttta ccatcattcc ttgcgaactg attgacagaa acggcgaacg tctaaaagaa
    18241 atcattttac gctacgcaga aaaatgggat ttagaacctg cttttgttga gtggttagaa
    18301 aaagaaaatg ttttctgttg tagtttagtt gatcgcatcg tcccaggtta tcctcgcgaa
    18361 gatgcaggtg ttttcaatca acggcataac tatgaagatc aattgatggt caaagcagaa
    18421 ccattcatgc tttgggtgat cgaagggcct aaagaattgg agaatcaact accattcaaa
    18481 caggctggct tgaatgtcat catcacagat gatatgactc cttatcgtga acgtaaggtg
    18541 catttgctta atggaccgca tacagcgatg gtccctcttg ctttgttggc ggaattggaa
    18601 actgtagaag aagtaatgaa agatccttta ttctccgcat atgttgatca gttattcaat
    18661 ctagaattga ttcctatgct ttctttacct aaagatgaat tagccattta tgctgaccaa
    18721 atcaaagaac gtttcttgaa tccttttgcc catcacaaat tagaagcgat ttcattaaat
    18781 agtgtttcaa agttcagcac ccgtctgctt ccagtgttca agaagtatat cgaagaacaa
    18841 aatcaggtac caccgttaat cactgtttca ctagctgcgc tacttcttat gtatcgaggc
    18901 gatcaagtaa agccacatga tgatgaaaag acgataagcg aatttactga tgcttggtct
    18961 gataatggaa cagctattcc tcgattattg caaaacgctg cgctttgggg agaagatttg
    19021 tctcaaatac cgaatgtgac agatacagtt caagaaatag ctgcaaagat cgatcaaaaa
    19081 ggtgtccggt cagtattaca agaaatgaaa ggaacgatgg tccatgcttg aaaccctgca
    19141 accaacaatg aaattaaatc gacgagacag tgtggtcgtc gcattaaccc ctattccaaa
    19201 acaaacacaa ctgacgattg atcagcaaac gatcaccact cttgaagata tcccgcaagg
    19261 tcataagatt gcattgggtg atttgaaagc tggagacaac gtgatcaaat atggctatcc
    19321 aatcggtcat gtgacgacag acgtaactgc cggtcaatgg ctgcacactc ataatgtaaa
    19381 aaccaatctt tctggtgaat tggattatcg atatgaaaaa gatgtccatc caagtcatta
    19441 cctttttgag aaccgaacat ttcaaggtta cttgcgaaaa aatggaaaag tagggattcg
    19501 taatgatttg tttttagtac ctaccgttgg gtgtgtgaat ggcattgctg aactgatcgt
    19561 caaacagttc aaggaaaaac atccagatct gggacagttt gaccatatta cgattttaaa
    19621 gcatccttat ggctgttcgc agcttggaaa agatcatcaa aatactcgag aaattttagc
    19681 ggacgcagta aatcatccca atgccggagg agttttagta tttggattag gttgtgagaa
    19741 caatacagtt cctgaattca aaaaaatttt aggtgaatat gatgaagaac gagtgaaatt
    19801 tctagtggct caagatgttt acgatgagat cgaacaaggt gtggctctat tagaagagct
    19861 tttaacagcg gcggaaaatg accagcgcat atcagtcccg ctctctaagt tgaatgtcgg
    19921 tttgaaatgc ggcggttctg atggtctatc gggaataact gctaatcctt tacttggtgc
    19981 attttctgac tacttgattg ctcaaggagg cagcacgatc ttaacagaag tgccagaaat
    20041 gtttggcgca gaacaagtat tgatggcacg agcggaaaat gaagaagttt ttgactcaat
    20101 cgttgacttg atcaatgact tcaaacagta tttcttgtct tacggggaac cagtttacga
    20161 aaacccatct cctggtaata aagcaggtgg gatcactaca ttagaagata agtctttagg
    20221 ctgcacgcaa aaatcaggaa gctctcctgt cgttgatgtg ctacaatatg gcgaaaaaat
    20281 cagaaaacca gggcttagtt tattgcaagc tcccgggaat gatcttgtag ctgcctctgc
    20341 tcttgcttca tccgattgcc agttggtctt gtttaccaca ggaagaggga ctccgtttgg
    20401 ctcatatgtc ccaacggtca aggtatctac gaatacaact ctttttgaac gcaaaggtca
    20461 ttggatggat tttaatgcag gcgtcttatt agaacaacca atggaagtaa tcttagaatc
    20521 gtttgttcaa aaaatcatcg ctgtagctag tggagaagaa acgaaaaatg aacaaaatga
    20581 cgttcgagaa attgccattt ttaaaaacgg agtaacactt tgattttcat aaaacagtat
    20641 aaaaagaaag aaggaagatt tatgttttta tctgatgatt ttttattaaa agacgattgg
    20701 gcgaaaaagc tttaccattc ctacgcaaaa aacatgccca ttattgatta tcactgtcac
    20761 ttagatccaa aagaaattta cgaaaacaac aatttcagca atctgacaga agcttggtta
    20821 ggaggcgatc attacaaatg gcgcttgatg cgagcttgcg gcgtgcctga agaaaaaatc
    20881 acaggaaatg cttctgattt tgaaaaattt ttagcttggt gtcaaaccgt accaaaaatt
    20941 atcggaaatc cgctttacag ttggtcacat cttgaattga agcgtttctt cggtattgac
    21001 ttgttgataa atgaagaaaa tgcagagaaa atttggacac aagcaaatga aattttggca
    21061 gctcctgatt ttcgccgtcg ggaaatcgca aaaaaatcga atgtagaagt gatttgtaca
    21121 acagatgatc ccgcagacga cttgcactat catcagttgc ttgctcatga agaaccagat
    21181 ttgaaggttc taccttcatt ccgtccagat aaagcactca atattgaaaa agaaggattt
    21241 aatgcatggg tcacactact ggaagaagca gctgaccaaa cgatcaccac ctatcaagaa
    21301 ctgatagaag tcttagggca gcggatagaa tacttccatc aacatggatg tcgtgtttcc
    21361 gaccacgctt tagacatctt acgctataaa gaagcggatg aaacagtatt agaagagatt
    21421 tttcaaaaag cacgagctaa caaactatta tcttcagatg aaatcgatgc ttatcgtaca
    21481 gaaacactga tacatttgac ccacttctat cattctcata attggacgat gcagctgcac
    21541 atccatgctt atcgtaactg taatacacaa atgttggaaa aattagggcc cgataccggc
    21601 tatgatggaa tgaatgatct ttctttgact attcctctgc aaaaattatt gaaccgtgca
    21661 gaagaaacgg atcagttgcc aaagacgatc ctttattcat tgaatccgaa tgactatcct
    21721 gcgattttag ctttgatggg ctgtttccaa aaagaacaga atggaaaact gcagttaggt
    21781 tctggatggt ggtacaacga tactcgagct ggtatgcgcg atcaattgac acaatttgct
    21841 gatggtagtg ctttggggaa ttttgtcggc atgctgacag attcaagaag tttcctgtcc
    21901 tacactagac atgaatattt ccgtcgtgtt ctttgtgaat ttttaggcga aatggtcatg
    21961 cgaggagaag cacctgaaga tgaacaactt ttaggaagtc tagttcaagc gattagttat
    22021 acaaatgcta aaaattactt tggctttttt gcctaattga ttaatagtaa cagtaacgaa
    22081 tcaaaaacag gatagtgaaa taacggaatc cgtttatcca cgttttagag aagaagacgg
    22141 accaaatttg ctccgtcttc ttctcttgct gttctgcttc attgcctata aacctttatg
    22201 aaagaagaga taacatgcct actatccata ttgccggtga ttctactgcg gcagaaaaag
    22261 atgaaaacaa gcgtcccgag acaggttggg gagaaaaaat ctctgcttat ttttcacccg
    22321 agatccagat caataaccaa gcaaagaatg gacgcagcac caaatcattt atccaggaag
    22381 gaaggttagc ccgtctagct tcacagtttc agccgaatga tttcttattt gtacaatttg
    22441 gacataacga ccagaaaatc caagaagaaa gaggtacgct tcccttcggt agctatttaa
    22501 aaaatctgca gatatttttt gaatgcgcaa aaaaagcgaa cgtccaaatg gtcttactga
    22561 catctgtgac gcgacgagat tacttagaaa acggaacatt gaatccagat atacttggag
    22621 actatccaaa agcgatgcgt gcatttgctg agaaacacca tattcctctt ttagatgtgt
    22681 tctcaagatc acaagaattg ttccagacct tctcaaaaga agagacaaaa aaattctacc
    22741 tgcatcttat acctgatact tgtaaaaatt acccagaagg actaaaagac aatacccact
    22801 ttagtccaga aggcgctgat aaagttgccc gaataattgt tgaattgata aaagaatccc
    22861 gtctgcctct tgctaattat ttacaaaaag gagtttgaga tgtttccatt aaatttaggc
    22921 atccgtgccc acgatttaga tgttcgaaat cgtgaagaaa tcgttcaaaa agtatcacag
    22981 cttcacttgt cccatatcca atttgcacca caaaaagcat ttcctgaaca acttaaatta
    23041 actgacatga cacttgggag tgctgcctat tttggtgaat acttccaaaa aaacaatatc
    23101 gaactttcca ttttaggatg ctatatcaac ctatccagca aaaatcaaga agttcgtgca
    23161 caagcattgg caactttcaa acagcaaatt gctctgtgct ctgcctatca agccaagatg
    23221 attgctacgg aaaccggtag tgtctctgtc ggttacacaa aagaaaattt tactgaagaa
    23281 gcttatcaga tcgccagaaa ttctatcatt gaattagtcg ctcatgcaga aaattttggc
    23341 gtaactgttg ctgttgaagc agggatcaat catccaatct ataactatca gttagctaaa
    23401 cgtttaatcg atgaagtagc ttcaccaaat ttaaaaatca ttttagattg tgcgaatttg
    23461 atgcataaag aaaatcatgc tgatcaggaa aaagtgatcc gaggtgcctt ggacaattta
    23521 catggttata ttacagcgct tcatctcaaa gactatatca tggaaaatgg aaagatccgc
    23581 atggtacctg ttggcaaggg ctgtttagat ttccgcccgg tactgcatta tatcaaatat
    23641 gagcgtcctc taatgtacgc cactcttgaa gctactcctg agatttatgt tcctgaagca
    23701 atagaacact tgcagtttat ttaccaagat tgttaaggag aagattatgg aggaacgaat
    23761 acagaaaagt tatcaaaaat atcatgcaaa tgcttttcag acattgctgc atctttatca
    23821 gggaaattat tggcgttttg ccatgtcagg actctttttt attatcaaac atcttccatc
    23881 ttggttaatg ccgatcgcta ttgccaatgt aatcaatgca gcttcatcga aagattcttc
    23941 tcagttgcag gccatttatt tcaacgcttt tttgatggct tgtctgattg gtcaaaatat
    24001 attgacgaat tattttcatg taaaatacca tagcacttcg atccgtcaag tagaagctgg
    24061 actaagagcg acccttatca agaaaatcca agaactatct attccttatc aaaaagaatt
    24121 gcaatctgga cgaatccagt caaagatcat ccgtgatgta gaagctatcc aaacgttgtc
    24181 ctctcaagta tttgtcagcg gattaaatat cgctgtcaat ttattcgtag ctttaggtat
    24241 cacgctttat aaaagtcctg ttgttttcat gtttttctta ttgactgttc ctctcgccgc
    24301 attcgttact ctctttttcc gtaaaaggat cagacagtcc aactcagact tcaggcaaga
    24361 aatggaagag acttctgcaa aagtgatcga agtgatcgaa ttagcaccga ttgctcgagc
    24421 tcatgggtta gaaaaacacg aaacaactcg cttaaataag cattttactc aaatctatga
    24481 aaaaggattc cacttagata tcctgcaatc tgttttcgga tcagtcagct gggctagttt
    24541 tcagatattt cagctgattt gtttgatttt cactgggata ttagctttac aagggcgcat
    24601 ccaacccggc gatgtcgtga tgtatcagac gtatttcacg acgatcgtca attccgtttc
    24661 tggaatgatc acgctgattc ctacgatttc aaaaggcatg gaatctatca cttctattgg
    24721 cgaactgttg attgctggcg atgtagaaac ttatcacggt aagaaaaaaa tccaacattt
    24781 ggatggaaat tttcgttttg aggatgtttc ttttctttat ccggactcac tcaaaccgat
    24841 tatcgatcat ttttctctag aggtgaaagc tggagaaaca attgcttttg ttgggccatc
    24901 tggctctgga aaatctacac tccttaactt attgattgga tttatccagc cgacttcagg
    24961 aaaaattttt ctagatgatc agcctttgga tgaactggat atgcgaagtg tgcgaaatta
    25021 tttagccgtt gttcctcaaa ccactttgtt gttctccgct tcgatcaaag agaacatcac
    25081 ttatggcttg aaaaacgtat caaaagaacg tttagatgaa gtcatcgaag ctgcgcaact
    25141 ttcttcactg attgaccagc ttcctgatgg tctggatacc ttagtaggcg aacatggcaa
    25201 caagctgtct ggtggccaaa agcaacgtat ctccattgca cgagcgttga tacgtcagcc
    25261 aaaaatcatt ttgctagatg aagcaacctc agctttagat aaccagtcag agaaaaaaat
    25321 ccagcaagcc ctgaattacc tgacagaaac acccactact tttattgtgg ctcatcggct
    25381 ttcaacgatc aaagaggcag ataaaatcgt tgtgatcgat gaaggaaaaa tcgttgaaat
    25441 aggtacttac gaaaaactgt tagctcaaaa aggctatttc taccgcttga aaaatgaatg
    25501 acaaaaaata ggagctatga caagactttt gtcgtagctc ctatttctga ataaacggtg
    25561 ttctcctgcc aactcctcgg tcacaagtca caatagtcat caaacatgag aagctgtttt
    25621 ttgaaattgt tccttgtaac tcagagttaa acggtaggct cacgacctct tggtaaatgg
    25681 tgttcaagag acagcctatc ggcaataaga agcaattttg tcatcgctca aggccgaacg
    25741 tctctttcac accctctgtt gtttcataaa atagttttat tcgctgtaaa ggtcgcgaac
    25801 tggtgtcatg atgatacggt tcaagtcatt gatgattgta ctgaagcctt gctctttttg
    25861 catcaagtca ttgattactt cttctgattg gattttcaca gccatttctt gtgctttcat
    25921 tgcatcttcg tcgctgaact cttgtccact catttgtttt tcttgtaatt cgatttgcat
    25981 cgcttggaag tctttgaaca gtttgtgtgc ttcttcgttt gcttgtactt tctcgtacgc
    26041 ttctttcaac gctttgaatt ctggtaattc acggatctca cgttcgattt gattcgcgct
    26101 atcgtagatg tttgacatgt tatgccctct tttcttttag tagttactct ctattgtaac
    26161 atggggttat tcgttttcaa aggggctact ctccttgata tttccgccag aggtctttag
    26221 ccgcttcacc aattccctga acgccttctt tcaccatatc gccgaagttt ttcagccctt
    26281 cttttgcttg ttcaccatat tgattcaagt tatcttgcca atttccagac tctccattag
    26341 aatttcctgt gtttccaact tgatctgcag ctaccacttg tcctcctgtg gcataggcat
    26401 ctgctacact aaatcctgtt tttgaagtat acggaaggat gccttctgcc gctgatttga
    26461 agactttccc gacaacattt ccacttgtcc cttccaaata gtgcagcttg ctggattctt
    26521 cgtaacccat ccatgtcgaa atcactacgt ctggcgtgta accgatcatc cattggtcat
    26581 ttgcttttgt ggcatcgaaa ttagtttctg tggttcctgt tttccctgct atcgtataac
    26641 ctgcaggatt cgcagcagcc gctgttccat ttgaaaatgt accaagaagc atgctggtca
    26701 tttcatcggc tgtttccttt gatatgacct gttttggttt tttgtttgtg ttatcgacga
    26761 tcaccgctcc agtcgaatca acgattttcg tgataagatg cggcgtatac atctgaccat
    26821 cattggcaaa tgcactgtaa gcacccgcca tgatcaatgg agatactcct ttttccagtc
    26881 caccaagggc taagccataa tacttatcag attttgctaa aggcaggccg aattcttctg
    26941 ctttatcata tcctttttgt aaaccgatct catgcaaaag ccatacagta ggcaaattca
    27001 aactttgtgc aacagcttga tacatcggta cttctccttg gtatgtcccg tcaaaattat
    27061 gcgcatcgta ataactttgc ggttcgtctt tcaagatact ggaaggcttg tatccagctt
    27121 ccagtgctgg tgtatatacg cttaatggtt tgatcgttga tcctggtgaa cgacgcatct
    27181 gtgtagcaaa gctgaatcct ctaaagacat gatctcctct tcgtcctact agtgcttgta
    27241 caccacctgt attcggatct aaagctactg aagcagattg gaccatggca ccatctgaag
    27301 catttgctgg gaataatgca ctgttttgat aagtagcttc taattgcttt tgataatcct
    27361 gatctaaagc tgtatagatt ttatagcctt tattcatgac atcttcttca tcaagaccat
    27421 actcgttaac tgcttcatcg atcactgcat caaaataata aggataacga taaccagaat
    27481 ctgaattatc gtatgtgtca ttaagatacg cgctgatatc tgtctggctt tctgcttgtt
    27541 cttgttcttt tgtgattttt ccgttttctt ccattacact tagtactgtg tttttccgat
    27601 cattcgcatt ttttgggtga tcgatcggat tataaatacc aggacctttc agcattcctg
    27661 ccaaagtagc agcctcagac agcgtaacat tcgttgcatc tactccaaaa tactttctgg
    27721 aagcgtcttg gacaccccat accccgttgc caaaataggc attgttaaga tacatggtca
    27781 agatttcttc tttgctgtac ttcttttcga tctcgattgc caaaaataat tccttcgctt
    27841 ttcgatcaaa cgtttggtct aatgtcagat acgcattttt ggcaagctgc tgagtgatcg
    27901 tacttcctcc gccccctgat gttccgccag acaagaccat tcggactgct gcacgggcaa
    27961 tccctttgat atcaaacccg tgatgctcgt aaaaattcct atcttctgtc gagattactg
    28021 catcttggat atagggagaa atcgcgttta attctacata tgttcctttt tgggcgtaca
    28081 gtgtgccagc ttcttcgttt ttcttatcat aaatgactgt cgattggctt aatcctgatt
    28141 ttaacgtttc gacatttgct tgttttgcta aaataaagag gtagatactt gtcactaaga
    28201 cacagatcaa accaatcagc aaaatcaatt tgttcacatg atattttttc cagatgcgct
    28261 ttcgccatcg atggaattgt ccgagatatg gtctgatcca acgccaaaaa ctgatcaaag
    28321 cagcttttat cttttctaaa atgctttgaa aatccatttg cttcttctcc tgtctatttg
    28381 attcaatcgt cttattttac catagtgaaa gagtgttttc ttcctgctta agttgtagtt
    28441 caatatgttt ttttacgtta gtgcaagtag agtaacataa gaatgaacag aaaaatgtaa
    28501 gttttttctt aaatatgttt taaaaatggt acaattagtc tgcgattgca ctgaaaggag
    28561 tacacttgtg aaaatcacga tcactttacc tatgaaccat cctgaaacaa caattcgcga
    28621 cttgcttgaa aaggaatggt tagttcctag aaaggttcga cactttttgc gtatacgtaa
    28681 aaacgtttgg atcaatcaac aacctgcttt gttccatcat gaagtaaagg gcaatgacca
    28741 gatcacattg ttattcgaag aaacagatta taattatcaa gaagttcaat taggagatgc
    28801 gaagcacatc cgtgttttat atgaagatga gcatttgatt atagtggata agcctgcagg
    28861 aatgaagact catccaaatg aaccgacaga aaacagtacc ttactcaatc atttagctgc
    28921 ttatctcgac actaaacagc aacgacctta cgttgtccac cgcttggaca aagaaacaag
    28981 cggtgccgtt ttatttgcaa aaaatccatt tgtcttgccg atacttggaa gaatgttgga
    29041 acaaaaaatg atctatcgga gatatcaagc catcgtatgg ggagaaattg ccaaagatat
    29101 gaccatcacc aataaaattg gacgagatcg ccatgatcgg agaaaaaggg tcgtagatga
    29161 taaaaatggt cagagcgcct tgacacatgt cactgtcaac aaagtacttt ctgaacaaac
    29221 acaagtctat tgtgttttgg aaaccggacg tacacaccag atcagagttc atttagctca
    29281 taagggacat ccgatcgttg gagaccctct ctaccaaaat agaacagctg aacgactgat
    29341 gctccatgca ttagaactgc acatgatcca tccatttact caagaaaaaa tagtcggtaa
    29401 agctttacct ggattatggg aatagaaata aaaaagaaca gagtcttatc agcagataat
    29461 gataagactc tgttcttttt gtgtaatttg tctgcttact tttgatgttc gtgttctgct
    29521 aacagcaaac taccgataat cccactttca ttcggcagtc cccaacgaac gatatattca
    29581 cttacttgag gtgtttccat ataaccaccc atcaattcaa caaattgctg acggattttt
    29641 tgaaggagat gatcctgatt cattacccca ccgcctaaaa taatcttttc aggtgctaac
    29701 gtcaacgtat agttgatcag tgcttgagca atataataag cctgaatagc ccaaacagga
    29761 tgatcttgcg gcagatcttg ccctttgatc cctgtgcgtg cctcaataga cggtcctgct
    29821 gctaatcctt ctaaacaatc tttatgataa ggacaagtac cttcataatg gtcatctgga
    29881 tggcgtttca cccaaatatg tcccatttca ggatgtgcga tccctgaaaa aatttcttca
    29941 ttgatcacga cacctgcacc gattcctgtt cccacggtaa ggtaaataca actatttttt
    30001 ccttgtgctg ctccttgctt caattcccca tatgcagcag cattgacatc cgttgtccaa
    30061 gcagcaggaa tattgtagcg ttccttgatt gtgccaagaa aatcgaattg tgcccaccct
    30121 tttttgggag ttgccaaaac gtatccgtaa ttatcttttg ttttgtcgat accaatcggg
    30181 ccgaatgagc caatcccgat tcccttcaat tcataacgat caaaaaattc aaatacctga
    30241 tccaatgttt cttctggtgt tgtggtaggg atacttaatt tttctaaaat ttctccacca
    30301 tctgaaaccg cacacacaaa ttttgttccg cctgcttcta ttccgccata cattcgtttt
    30361 cctcctcgaa tccgattgtc cgaatctcat aaggttggat tattgggaca accgcttgct
    30421 tcgttggttc ttcaagcaga ttcaatatga cagtttttcc attgtcttta gttatttcca
    30481 atttttctaa atgactagac atattgaaac ctcttactac tactttatca ctaaacttgc
    30541 tgcgtttcaa agctgtaatt gcaaacgttt cactttttat cgttaaatat tgttgttttg
    30601 aaatcaatgt tccttcatga tgtttgattt gttgagtgga aaaaggaatc tgtgctgcgt
    30661 atgcatgcaa ataagtagag aatttttctt ctggtccatg tagttcaatg ctgtaattga
    30721 accgatgctc acctagacac tgtgcttctg gtgttggaaa atatccccaa tcgcctagtt
    30781 ctcccacact gcgaagtagc gtcactgcga tttgtccgtc ttctgttact tcatattcat
    30841 tcaaaccaaa gtttccgact gttacaccgt actcttcatc atgaatattg acaaatgctt
    30901 gctgatgttg cggattcgtg gggttttccc aacttttaga cacatggttt gggcgcttca
    30961 ccacttcaaa aatactatct gcttcgtgat gttccacatg taactttgta ggaaacaaga
    31021 cacgtaagcg atgatctttc atctgattgt cgagagtcgt ttcaaagtcg atttttttac
    31081 tatcttttct gattgtcatg attgttttta tttgaaggat tcgtttttct ttggatcgtt
    31141 ctgcttttcg ataacgaaat tccacaacca tctgctgttc tttttctaaa cgctcgtctg
    31201 cagaaacagg gatttccatt tcctgtagaa tacttatttt tgctatttcc ggtgtatcta
    31261 ctaatacttt tacttcagag ttttctttgt tactggatag gattggctga tcacagaaag
    31321 gttgtttaaa gatatactca tttcctatat cgccgatatc ttcaaaaata tgcagattat
    31381 tcaactttcg tttgtttttg cgatcttcca ttgtaatggt accatttttt tcaatcgtca
    31441 aatgtagagg gccgttttcg atgattcgtc cactctgatg aatcattgtt tccttttctt
    31501 ctgctttcgc ttccccttct acaagcgcga atgtttccca agaaaatgct ggcatgtttt
    31561 ctaatggtaa agtcactttg atcattcttg ccatatatgg cacacggaaa cggtctttcg
    31621 gcaaatcata accaaattgg acagtttctt cagataaaat agctggtata gtggccccac
    31681 tttttgtttc tacatgatac ttacgtttag gcaaattttc taactcttga tatagttgtt
    31741 caggtattcc ttcagcgaat ctttttcttt ctaattcgat ggttatttct gcttcaccag
    31801 ttttatccat tcctgcagta tttacaatga caaatggaaa gctgtctttt gggaatccgg
    31861 tcgtatcgat tgctgcggtc agctgttcta aggaatcttg tgcaagatac tttccaactt
    31921 catctgcttt ttcaaaacga ggtatcattt cccgatggac agaatcgact gaacaaccgc
    31981 aaatactgtc gtgaggatga ttttgcatta atgtcttcca agcataatct aattgatcat
    32041 ggggatagtt tccactaact tcataagcca tcgtcgcaag tggttctgta atattttcta
    32101 gctgtcgctg gaccttggta ttccattgtt ttaagtaaac ccgagcagat gctgtattgg
    32161 ctaaagtata ccagccatcc gtttcttgac ttgtcaattc tccttctact gagcctaagt
    32221 cttctggcac atctttttga actgcttcca gatagtcagt aaaattagaa tgaataaact
    32281 cgtaatctgg aaacagttca tttgcgagat ggattgcttt tgatatatct ttttgtaccg
    32341 gttgatgatc aacaccattc atcatcaata agtggttcgt agaggcatat tgttctgcat
    32401 ccgctaattt ttgcttccaa aaggcaagag ctgcttcttt ttctactgga atctcattcc
    32461 cgttgctata ccaattggca aatagcagac caaagattgc tgtttgatca ggtcctttcc
    32521 accacatctc tgagtattgg gaagaatagt tttctgcttc caaaacttga ttatcaaagc
    32581 caattggttt tacaccacgg ccaaacgctg ctgctgaaat accagcttgt ttcattaatt
    32641 gaggcgtctg tcccatattt ccaaatgtat cgggaaaata gcctaacatg acaggggttc
    32701 cccatttacg gctttcttcc atgccaatca gcatatttct cacgttcgat tccgagctga
    32761 taagaaaatc atcttgcaag atataaaatg gtccaattct caattttcct tcattgattg
    32821 cttgttggat tgcctgtctt ttttctggcc ttacttgaag atagtcatct aaaataatcg
    32881 tttgcccatc caaatgaaaa ctgttgaagg acggatcaac ttcaaatagc tctagtagat
    32941 cgtctatcaa ttcaatcagt cgcatgtgat gctgttcgta agccatgtac cattcacgat
    33001 cccagtggct atgagaaatg atatatactt tctttttcat ttacgatacc tcctactttt
    33061 ctactctaat atcaaagtaa tccatgacta attcgcaaaa catcatattt gcccaagaaa
    33121 accattctct cgtataattt tcaggattgt tcacatcgaa tccttcgtgc ataaggtgtg
    33181 ttcctgcgtc agttgctaca agtaaattca atatccgctc tttttcagat ttgtctgacg
    33241 ttgtcattcc ttccatagct aatgcaatcg gccaaacgta tttttctggc gtatgggagg
    33301 atccaattcc ttttgcgtac tttccttcat agaaatatgg attttcttta ctcaacaatg
    33361 ttcttcttgt ttgcagataa tcttcctctt gttcagaaca atatcccaag taaggtgcgg
    33421 cgatcagatt gggcacatta ctgtcatcca tgactgtggc gtttcccatt ccatctactt
    33481 catatgcata aatcgtttgc cctttttgat taaccgttcg tccgtgattc tgaatcccct
    33541 cttggatctc tttcttcaat ttctgtgttt gctgtacaat ttctttatca tcgagtatcg
    33601 tttgaaaaat ttcttccaca taacctaaca cgaccaccgc aaacatattg gatggtacca
    33661 gatatccata acggcaagta tcatcgctag gacggaaacc agaccatgtc attcctgtaa
    33721 aagctgtttt tgcacctcgc ccgtcattga tcagcgtatc ttcttttctt gtcgtgtctc
    33781 gtacaaatgt ataaggagag tgttcatgat cttgctctgt tttgaacacg tccaagattt
    33841 ttttgatacc ttcaataaaa gattgattga attgatcagt ttttcctgta tttttgtaaa
    33901 gaagataggc caactgtatt gggtagcata aagaatcaat ttcatatttt ctttcccaga
    33961 tccaatcgtt catttttgta tgatctgttt ggtggccagc accgttagct tcttcgttga
    34021 aggcattcgc atatggatcg atattgatat aaaaaaattg ctttttgact aacccgctga
    34081 tcatgtcagc taattcctca tcttcttttg caatcactaa ataaggacga acttgtgcag
    34141 tagagtctct taaccacatc gctggaatgt ctcccgtcaa taaaaaggtg gttccgtcat
    34201 tttgcttttt gactgttgtc aacagagtat tggcaaatgc tgctgtaaaa ttctttgccc
    34261 agtccgcatg gatatcccca catttttgtg tgatatcttc cataaatttc tgcaccgatt
    34321 ttggtatatc tttataagcc actaataaaa ccttctttct atttgatata tctttaaaga
    34381 ttatacttca tacagccaat tatgtctacg ctttcaaaaa atatcttttt atttactttt
    34441 tcatccatta tggtattatt tgaatatgat atatcattaa gaggtgttta tatgaatcaa
    34501 ccattgtacc agacaatatt taatgatttg caaacggcta ttttaactgg tgaattacct
    34561 gtagacagcc aactaccaac tgaaaaagaa ctttcacgta cctataaagt aagcagaatc
    34621 acttccaaac gtgcattgac tgaattagag caattaggac ttatttatcg cgttcgcggg
    34681 aaaggcagtt tcgttaaatc cgcttctccg gaacagattc ttgcttctcc tacaaaaaca
    34741 cgacggattc tgtttcttct tccatatgtc gctgatttgt ctgttggtga ttttagcaag
    34801 ggattgaacc ctgtgatgca agataaacag attgatgtca tgatgacgac gcttgacttt
    34861 ctacaaaata aaacagcaaa agagctaatc aatgaatttg acggattgat ttattatgct
    34921 cttgatacag aacaacatct tgacttactt tttgagcttt cattgaaaga attccctgtc
    34981 gtcatattag ataaaaaaat ctatgagtta ccttttccta ccgttctttc cgataatttc
    35041 caaggcggtg cgcttgctgc ctcttatttg atcgaacagg gacattcaaa aatcgcttat
    35101 ttgtttggta cgcagactca tccacaatcc gttcgacagc gttaccttgg ttaccttgaa
    35161 gcattggaca aagctggttt gactttccac acttcactgg atgacaaaag cgcgacatta
    35221 gatgaattgc tttcttatat cactaagtat gaagtaactg cttttgtttg tgaaaacgat
    35281 gttgtcgcta tccagaccat gaatctcttg agacagcatg gttatcatat ccctgaagat
    35341 ttttcaatta tcggatttga tgatatccaa gcagcagctc ttgttgaccc gccactgaca
    35401 accatcgcac aagattttga acagttaggt aagcttgcgg gagatgcttt ggtcacttgg
    35461 ttagagacaa aagatcgacc atccgatagt aaatttccag tttcattcat caaaagacaa
    35521 tcaacaaagg agaaataaaa tgaatatcgg tcaaatagat actagacatg caacagcgaa
    35581 tcagccaaat ttctctaatg ggaactgcca gccttatact ggcgttcctt ttgggatgaa
    35641 ttattttgca ccgcaaacta cggatcaaaa cggcagctgg tggtttcatc ctgatgaccg
    35701 cgtttttcaa ggctaccgtc ttacccatca accaagtcct tggatgggtg atttcagtca
    35761 tatgctgttt acgccgatca atggaaaact acaggagaac acccttttcc atgctcaaag
    35821 ttcttaccgc ccagaagaaa gtatattttg cccgactcat ctgtcgatct gtcaattacg
    35881 ttatggcatc tgttctacat tgatacctag catgtatggc ggaatcttat ctattgatta
    35941 cagcaaaaat gacagcggtt tacttttatc ctttccggga cgccatcagc tctttgttat
    36001 agatccttac acagtggaag gaaaaatcac acattttgct ggttcagaag atcctgattt
    36061 ttctttttat tttatattga gatttgaata tcctttaaca attgatgagt tagctgtttc
    36121 caacaaaaat agcgactcga tcccgttgta ttttgaacag acaaaaaaac aaactatccg
    36181 atttgggaca tcttttatca gctcggaaca agcagcttat aacctggaac tagaacaaaa
    36241 ccaaagcaaa gagacttacc ttgctcacag tcaagaacaa tggcagtcct attttgatcg
    36301 tatacagctc acacaccatg atcaacaaca attgcggacg ttttatcata atttatatcg
    36361 ggtgttttta tttccacaaa cattttatga aattgatcct agtgggcaaa aaatccacta
    36421 tgatacgact agtcgatcta taaaacctgg tgttctctat acaaataatg ggttttggga
    36481 tacgtataaa acggtttatc ctctttattc tttgattgct gtagaaaaat acgaagaaat
    36541 gctggaaggt tttttgaaca gctatagaga aagtggctat ttgccaaaat ggctgtctcc
    36601 tgatgaacgt ggacttatgc ctgggacatt gatcgatgcg gtcattgcag atgctgctgt
    36661 aaaagatatc cgacgagact tgatgcctga atttctaacg gcaatggaaa aaggtgctac
    36721 ggttcaaagc aaacatccaa attatggacg ccaaggaaca gaagattact tgacatatgg
    36781 ctatgttcct aatcactacc acgaatcagt caatcacaca ttagactatg cttatagcga
    36841 tttttgtatt agccaagttg ccaaggtact agaagaagag gaaatgacga agaaatatca
    36901 gcagcaatca ttgaactatc aaaatatctt tgactatgag tcaggattta tgcgttcaaa
    36961 agataaagat ggcaagttca gagaaccatt ttcagctaat cgttggggac aagattatgc
    37021 agaaggaagt gcttggcaaa gcagctttgc tgtttatcac gatttttcag gactgatcgg
    37081 aaaatatggt ggcagtgaga gatataaaga aaaactcatt gagctgtgca atcagccccc
    37141 aacgtttgac ccaggaggat atggttttga gatccacgaa atgagtgaga tggccgcgat
    37201 agaatttgga caattagcta tttcaaacca gccaagtttt cactatcctt acttgttcag
    37261 ttatatagga caacctgaaa tggcacagcc actgcttaaa cagttgatga ctcagacctt
    37321 cgacgattct ccaatgggtt accctggaga tgaggacaat ggaagtatgg ccgcttggta
    37381 tatcttcaat agtctgggat tttatcctgt cacgcctgga accggtgaat atgttatcgg
    37441 tatgccatta attgacaagg ctgttttgct tttgtcaaat ggaaaggaat tgacgattga
    37501 agcaacgcct aatcggccgc agcaacaatt tatccataaa atcaaacgga tggacaaaga
    37561 atatacgaaa ttatatttta cccatcaaga tttgctgaaa ggcggcacga tctcttacca
    37621 attagggatc atccctcacc ctaagaaata tcaagcaaaa gatttgcctt tttccttatc
    37681 aaactataat taacaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc
    37741 aagcaaaaat agatcctcat atttttcgtt tggttggtct tattaccgca gatgcaagct
    37801 tttgaacacc gtttattgag aggttgtgaa atcagcgttc tgacctgagt attagagaga
    37861 caaatggcaa aatagttcct catattttgc cattttttga gctaataccg agggtcagct
    37921 gattgaacac cgtttagtgg agaggtcgtg aaaaagctg
//
LOCUS       DACEPD010000016        37288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.016, whole genome shotgun sequence.
ACCESSION   DACEPD010000016 DACEPD010000000
VERSION     DACEPD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.016"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            107..970
                     /locus_tag="IW797_000830"
     CDS             107..970
                     /locus_tag="IW797_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1223622.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1099..2721)
                     /locus_tag="IW797_000831"
     CDS             complement(1099..2721)
                     /locus_tag="IW797_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1223623.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2970..3491
                     /locus_tag="IW797_000832"
     CDS             2970..3491
                     /locus_tag="IW797_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223624.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IW797_000833"
     CDS             complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IW797_000833"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ1223625.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5572..6390
                     /locus_tag="IW797_000834"
     CDS             5572..6390
                     /locus_tag="IW797_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ1223626.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6493..6690)
                     /locus_tag="IW797_000835"
     CDS             complement(6493..6690)
                     /locus_tag="IW797_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223627.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6859..7437
                     /locus_tag="IW797_000836"
     CDS             6859..7437
                     /locus_tag="IW797_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1223628.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8482..8985)
                     /locus_tag="IW797_000837"
     CDS             complement(8482..8985)
                     /locus_tag="IW797_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1223629.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9343..10368
                     /locus_tag="IW797_000838"
     CDS             9343..10368
                     /locus_tag="IW797_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1223630.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10691..11569
                     /locus_tag="IW797_000839"
     CDS             10691..11569
                     /locus_tag="IW797_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223631.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12045..12161
                     /locus_tag="IW797_000840"
     CDS             12045..12161
                     /locus_tag="IW797_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1223632.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13089..13427
                     /locus_tag="IW797_000841"
     CDS             13089..13427
                     /locus_tag="IW797_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223633.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13521..13751
                     /locus_tag="IW797_000842"
     CDS             13521..13751
                     /locus_tag="IW797_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223634.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14151..14522
                     /locus_tag="IW797_000843"
     CDS             14151..14522
                     /locus_tag="IW797_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ1223635.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14796..15488)
                     /locus_tag="IW797_000844"
     CDS             complement(14796..15488)
                     /locus_tag="IW797_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1223636.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15491..16276)
                     /locus_tag="IW797_000845"
     CDS             complement(15491..16276)
                     /locus_tag="IW797_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1223637.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16513..17913
                     /gene="lacG"
                     /locus_tag="IW797_000846"
     CDS             16513..17913
                     /gene="lacG"
                     /locus_tag="IW797_000846"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ1223638.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17938..18255
                     /locus_tag="IW797_000847"
     CDS             17938..18255
                     /locus_tag="IW797_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ1223639.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18268..19941
                     /locus_tag="IW797_000848"
     CDS             18268..19941
                     /locus_tag="IW797_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1223640.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19955..21586
                     /locus_tag="IW797_000849"
     CDS             19955..21586
                     /locus_tag="IW797_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ1223641.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22077..23642)
                     /gene="guaA"
                     /locus_tag="IW797_000850"
     CDS             complement(22077..23642)
                     /gene="guaA"
                     /locus_tag="IW797_000850"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ1223642.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(23701..24624)
                     /locus_tag="IW797_000851"
     CDS             complement(23701..24624)
                     /locus_tag="IW797_000851"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ1223643.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            24820..25425
                     /locus_tag="IW797_000852"
     CDS             24820..25425
                     /locus_tag="IW797_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1223644.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            25733..26749
                     /locus_tag="IW797_000853"
     CDS             25733..26749
                     /locus_tag="IW797_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1223645.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            26890..28191
                     /locus_tag="IW797_000854"
     CDS             26890..28191
                     /locus_tag="IW797_000854"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ1223646.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28237..28899
                     /gene="deoC"
                     /locus_tag="IW797_000855"
     CDS             28237..28899
                     /gene="deoC"
                     /locus_tag="IW797_000855"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ1223647.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            28920..29318
                     /locus_tag="IW797_000856"
     CDS             28920..29318
                     /locus_tag="IW797_000856"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ1223648.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29384..30466
                     /locus_tag="IW797_000857"
     CDS             29384..30466
                     /locus_tag="IW797_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1223649.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            30678..32240
                     /locus_tag="IW797_000858"
     CDS             30678..32240
                     /locus_tag="IW797_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1223650.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32233..33366
                     /locus_tag="IW797_000859"
     CDS             32233..33366
                     /locus_tag="IW797_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1223651.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33366..34322
                     /locus_tag="IW797_000860"
     CDS             33366..34322
                     /locus_tag="IW797_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1223652.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34421..35587
                     /gene="deoB"
                     /locus_tag="IW797_000861"
     CDS             34421..35587
                     /gene="deoB"
                     /locus_tag="IW797_000861"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ1223653.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35611..36429
                     /locus_tag="IW797_000862"
     CDS             35611..36429
                     /locus_tag="IW797_000862"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1223654.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36446..37153
                     /gene="deoD"
                     /locus_tag="IW797_000863"
     CDS             36446..37153
                     /gene="deoD"
                     /locus_tag="IW797_000863"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ1223655.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        11629 a         6509 c         7891 g        11259 t
ORIGIN      
        1 ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct
       61 ataatagacg ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt
      121 aacagattct tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt
      181 atcaatgaat ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa
      241 ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa
      301 tgttggccga tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata
      361 tgtggctttt tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt
      421 gctgaaggaa gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat
      481 tggggaaggg cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga
      541 agaaacccta gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga
      601 cgatctgaaa tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag
      661 tctattgagt gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga
      721 caaagttcga ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat
      781 cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga
      841 gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt
      901 aggaccaacg atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat
      961 cagtcgataa aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa
     1021 cagcgactct taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt
     1081 tttaagctaa ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt
     1141 ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc
     1201 aatgatgcgg tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga
     1261 gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt
     1321 cgttggatca tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca
     1381 acggactttc tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa
     1441 caacattctt cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata
     1501 ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg
     1561 taaataagaa ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc
     1621 acccatgata actttgaata gcgttgttgt tgttatatca ttttctgcga cgaaagcgac
     1681 cttgtcttct ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt
     1741 tacacttaca ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc
     1801 aacgaatgga taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat
     1861 ttttttacgt gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa
     1921 ttcttggagt tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc
     1981 cgctagttga ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc
     2041 aaaatctaga tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac
     2101 aacgatcaca gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat
     2161 atccagaccg tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg
     2221 tgctaataac acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa
     2281 ttcttcaggg atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc
     2341 attaagctca gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa
     2401 atcctctttc atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc
     2461 catgatgact gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac
     2521 ggccatccgt tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga
     2581 taacacttta aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt
     2641 tccgggggta aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact
     2701 cacatcattt acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt
     2761 cttatagaat tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt
     2821 tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg
     2881 atttattagt ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa
     2941 ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct
     3001 cattgatttt gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg
     3061 gaaatttagt tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg
     3121 aactgtttgt acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa
     3181 taatcgggat catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag
     3241 tcgcagcatt gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat
     3301 ttactgctga cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca
     3361 atattttagg aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga
     3421 cattaagctt tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg
     3481 catttagata agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt
     3541 tgtaattgga agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt
     3601 cgagacaaat ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg
     3661 aagcatagcg cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat
     3721 ggacttcata tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta
     3781 aatgcagatc ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag
     3841 gacgatctga ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg
     3901 taaagctatt tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata
     3961 caccaaattt cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaatcat
     4021 tcgaaaagac aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca
     4081 tgaccatagc aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta
     4141 gttgtgcggc aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg
     4201 tatgtttttg tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga
     4261 catctgtgac gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct
     4321 ctggacgaat cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt
     4381 ctttattttc ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga
     4441 caagtctttg caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc
     4501 gtccaaattt tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg
     4561 cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt
     4621 catttccggg tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag
     4681 gaataattcc ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt
     4741 ctttgatttg cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg
     4801 ctttttcaag ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg
     4861 ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa
     4921 cagggatagt cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta
     4981 ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga
     5041 aaataggatt ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt
     5101 gtcctaagaa cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca
     5161 aatgtgtcgc accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg
     5221 catctgcagc ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt
     5281 cggttttttc ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt
     5341 aaatatggtc gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt
     5401 ttgacatggt gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta
     5461 tagaactttt tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt
     5521 ttgggaatat gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa
     5581 tggctattga gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt
     5641 atgacagctc ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta
     5701 aacttgatgt taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta
     5761 gatccggcta ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt
     5821 gggtttggtt taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat
     5881 tttggaccac aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt
     5941 gttttttcta ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcggt
     6001 gcagcagcaa ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta
     6061 ggtattggta tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa
     6121 ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt
     6181 gctggagtga ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt
     6241 gcttttgccg caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag
     6301 cagacattgt ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt
     6361 atgatgattt tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata
     6421 gggccgcgaa cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa
     6481 tggtatcata cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag
     6541 ccagatgata agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag
     6601 gacactgaca aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa
     6661 gatatttaag aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact
     6721 atagaaaaag tataacaggg attgtttaaa aacacctcca actgaagact gatttttact
     6781 tcatctactt aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg
     6841 gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc
     6901 gcgattatca tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag
     6961 gcgctcgctg cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg
     7021 atttattgga acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc
     7081 ttatgggcga acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg
     7141 atcagtgagt acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc
     7201 gctaatattg cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag
     7261 aaagcattga aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat
     7321 gtgttaggaa ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc
     7381 gtgattctag ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat
     7441 gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc
     7501 gagtgatact agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact
     7561 gattactagt tttgtcacga tgcttattta tttggatcaa ggtcatttga cttggacagt
     7621 agttggtgtc gtaggatttt tgacattatt cacagcaata aaaaaataaa catctttatg
     7681 taatctgatg ttctatctat ttaagaaaag catgtttaca gcagtatgat tctgttgtaa
     7741 acgtgttttt atttgtggaa aatttaattt ttagaaaagt gtatcgaaaa atagatagtt
     7801 agtttttcca cagtggataa cttcgtaaga gataaaaata aaaaattagt caaagaaggg
     7861 cgtatagatt tcttttgata ggagatatac aacagaaatg aataaatatt aacgtgtttt
     7921 caaacagtat aaaacggtgt ggtttttgga ataaaaaaat aacatatctc attttacact
     7981 tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta
     8041 ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca
     8101 gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat
     8161 ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta
     8221 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
     8281 taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt
     8341 ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt
     8401 tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac
     8461 ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc
     8521 tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt
     8581 tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt
     8641 attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc
     8701 tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa
     8761 attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg
     8821 taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta
     8881 agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag
     8941 aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat
     9001 ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac
     9061 caacgtagtt atcgtacgaa aattagaagc tttttttgtt gggaatccgg tttctttaac
     9121 aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca aaacgactcg
     9181 gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac taagtttatg
     9241 aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg gcatttcatc
     9301 ttggttcagc tttttttcca attactagga aggagggata gattgttcaa gacttatttt
     9361 cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct acttatcttt
     9421 attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt ttcatcatta
     9481 gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct gaaacgtatg
     9541 tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc gacttatgtt
     9601 ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt cgtgttgcga
     9661 ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc catgggaacg
     9721 agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt tatgggtgtc
     9781 gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc tgagcagaca
     9841 attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat cattgattct
     9901 gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt gaatctgcat
     9961 aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa cttggatgcg
    10021 atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg tgtgtcggca
    10081 ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac ttttctatat
    10141 ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt tcttaatccg
    10201 atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt gatcgaatat
    10261 cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg ggtaatgacg
    10321 gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa ggacaaatat
    10381 atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa ggtgttcaaa
    10441 agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa ctttcaaaaa
    10501 aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat aaactttcct
    10561 gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata gaaagtttaa
    10621 gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg gataatcagt
    10681 catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga aaataagcaa
    10741 tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag agcaaatgat
    10801 tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg aactaatgga
    10861 tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag aggaaccttt
    10921 tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa tttatcaacc
    10981 attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta gatttattca
    11041 tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga tttggcatta
    11101 cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat attttccgaa
    11161 agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata ttccttccga
    11221 tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc gtttttttga
    11281 actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt tactatcacc
    11341 caatcttact aaatggttga aaaaagagtc aaaaatctgt ctttttgcag ccgattatcg
    11401 attattatca gttgatgaac aggacgatag tggagtccct tggatctttg gacgagtaga
    11461 tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa aagagcaatt
    11521 gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag tttagtactt
    11581 cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc acgctctaaa
    11641 cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
    11701 tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca
    11761 gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac tagctataac
    11821 aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa cgtaataatg
    11881 aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt ttaacaagtt
    11941 gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt tcgatttgtg
    12001 ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa aaaacagata
    12061 tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa cgtgcgctga
    12121 agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga aacttaatta
    12181 aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg tgtaaggttt
    12241 ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta gaatctttct
    12301 tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa ccacaaagca
    12361 cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa cctcttattt
    12421 tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg aaacattttt
    12481 agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt catgaaatat
    12541 ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata agcagttacg
    12601 aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa aaaaatcagt
    12661 gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg ccgaaaaaat
    12721 atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa taataagttt
    12781 tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag taataaagta
    12841 tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa ttaatggtta
    12901 aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg tgatactaga
    12961 ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa tgaaaaagaa
    13021 gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt agccaagtaa
    13081 cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt cgtgctacag
    13141 aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct agtgagccaa
    13201 ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt atggtggtaa
    13261 ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt gaaaaggtga
    13321 gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc attttaacgg
    13381 attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca ctagtgaaat
    13441 actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa tcgatcatca
    13501 aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt tattacgtga
    13561 atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt cgctactcaa
    13621 ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa actgtttttt
    13681 ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat tatgttagta
    13741 tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt tattgttttt
    13801 tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta tccagagtat
    13861 tttattttaa cttttgtata tttagtaagt agctacacac ttctatataa attcagaagt
    13921 tcttctagag tttctaaagt ttgtttctta attagcttat tgattctata atatttttga
    13981 tttatatagg aaattaaaaa tattatagaa tcaacactca gagaacgggt tggtttttaa
    14041 tagatttaat aagtactgat aatgaaattt ctcctggttg ggaaaacgaa gttggttacc
    14101 ctgtagaaga attttttcaa gattactttg cggagtatgc cgataggata atgagagatt
    14161 acgtaacaga taaaactcaa gaagtcatca atcaatccca gtcattaaat gaattatctg
    14221 ataaggcgaa agaagaagca gttgccatta tttcttcagg gattagtgac tatattgata
    14281 tgagtggtga aatcaattat ggcgataaag acaaaattgt acgcatgata aaagctttta
    14341 tcaaaacttt tgatgtgaag gcagatctta agtttaatga tacttaccta gtaggaaaat
    14401 taattaatat ccttgggaca gagaaagata cggaaacgtt tatttcctct ttgtctatgt
    14461 tgtttacaga taagcatttt tcaactcagc ttggtggaga ggaactagtg gaaatctttt
    14521 aaccattacg cccagcatta ataaaagtat atcaaaaaag tactcctgat gatttttaag
    14581 attggtttga gaaataatgt gaaaacacta tttttaatgt taattataga tagagatgaa
    14641 cgcgtcatat ggttaaaaga atgaaaaagt caaagataca ctgctatatt tcactaagga
    14701 tatagcagtg tatctttcat gtcgtttcac tggcagtttt ttataaaagg aggtgtgcat
    14761 ttcagttgct tttctttggt agttagttga atcgtttaag aattaacgga tgtggcagac
    14821 aggtgctctg tccaaaaacc agtaaaatct ttgatggttc catctttatt tagctgtgcc
    14881 tgttcaaata ctgttttgtt tttaccgaat ggatcaattg caataattgt tccgatcccc
    14941 gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga taacaagaca atcagccgga
    15001 gctaagctca gtttgtctgc cgctttaaga aaaatatccg gttgcggttt cccagggaaa
    15061 gagccgtcgt catagacaat cttgtcccag tcaaaccagc gggctaaatc gaaaatatca
    15121 aaataaaaag caacattctc tttgacagta gccgtggcaa tcgttaatgg aatctgttgg
    15181 tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca actgaagctc gttttcattt
    15241 tgcaagcaaa gcatacgata atagctttct ttttcttctg acaatacggc gatctcagag
    15301 gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg tattcgtccg accatgaata
    15361 ttttttaaaa tatcctcgtc tgataaacca tcttttgtat gatcatgtat catatgcagc
    15421 caagcagctt catgcagatg agagtccaaa aacattgttc cgttgaaatc aaatataatc
    15481 cctttcatag ttacgctcct atagtggtat tttcggttga atcagcaatt tgcagatcga
    15541 catattgtgc atagtgctct tgaacctccc gcgagatctg gcgatccgta atgagcagat
    15601 cgaaatcatg caaattataa aacacataaa aatcatctcg attgaatttt ttattatcaa
    15661 cgagtagata tttcttacgg gaattattta gagcgatctg ctgtgcttct ccttcttcgg
    15721 tactgtaagt agagatcgct tcgttgtgaa cgccatttga actgataaat gcttttgtaa
    15781 actttagtct tcttaaattg tcgttggtga tcggtccgac aaatgcgccg gtatttttcc
    15841 ggaaatcacc gccaatcagt agaatttctg gtccatctgc ttgagcaaga atatcaaaaa
    15901 ctggataatt atttgtaatg atccgaattt tcttgttcag taaatgcgtc gccaatagtt
    15961 cgattgttgt acctggtcct aagaaaatcg tttctccctc gtgaatcaag gaggctgctg
    16021 tggcagcaat ttcttttttt tcttgaatct gcagagtggc tttttcagtg tgagaaagtt
    16081 cttgatctaa agtgtaggaa agactttgag cccctccatg gacacgcagc agcttgcccg
    16141 atttttctaa ttcatctaaa tctctgcgaa cggtcatatc agaaacatct aattgattga
    16201 tgatttcatt aacggtaagg attccttttt tattgaccat ttctacgata gtaagcaatc
    16261 tttctttttt caacattgtc ttcacctcta atttaaggat aatctatcat ataaacggac
    16321 aacagtcaat ctaattcgaa caaaagtaaa catttagggt ggtttttatg tttgttacaa
    16381 tagattttat tgctgttttc tggtttttat gattatttgt gtttgttttt gttgacaaat
    16441 gtttcggatg cgtttacaat gcaatcagtt agaaagagcg aaaactgatt atagatttga
    16501 ggagagatgt acatgcttaa attaccagaa gattttattt ttggcggagc cacagcagcc
    16561 tatcaggttg aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg ggatgacttt
    16621 ttagaggaac aagggcgatt cagtcccgat ccggcaagtg atttttatca tcaatatgca
    16681 aaggacattg aattgtgtga acgttttggt gtaaatggtc taagattatc cattgcttgg
    16741 agccgcattt ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc tttttatcat
    16801 cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt gcatcatttt
    16861 gataccccta aacgtttgtt tgaccatgga gattttttga atcgagaaac aatagaagca
    16921 tttgtttcct atgccatttt ttgttttcat gaattcaaag aagtcaaggt gtggagtacc
    16981 tttaatgaaa tctatccagt tgcaaccaat caatatttgt taggtgtttt cccaccagga
    17041 atcaaatatg attttacaaa gatcgtcgcc tgtcttcata atatgatggt tgctcatgcc
    17101 cgtgtagtga actatttcaa agaaaacgaa cttccgggtg aaattggtgt agttcactca
    17161 ttagagacga aatatgcggc aacagacgcg ccggaagata agcatgctgc ctttttagac
    17221 gatgcattgt cgatccgttt cttattagat gctacctatt taggctatta ttcaactgaa
    17281 accttgactg ctcttgatga gatatgtgaa gcaaatcagg ccagctatca ttttcctgaa
    17341 gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg actacttagg gatcaatcac
    17401 taccaatgcc attttgtcaa agcttacgat ggagaaaatg caatccatca taacggtacg
    17461 ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg gagaacgtat ttataaagaa
    17521 ggaattcctc gaactgattg ggactggctg atttatccag aaggtctata tgacttgttg
    17581 ctgcgaatta aaagcgatta cccacattac aacaaaatct atatcactga gaacgggatg
    17641 gggtataaag atcagtttga agatggcatt attatggatc agccgagaat cgactatttg
    17701 agagtctatt tggaatcctt aagcaaggcg ataacagccg gggtaaatgt gaaggggtat
    17761 tttttgtggt cgcttatgga tcttttctca tggacaaatg ggtataacaa aaggtatggc
    17821 ttgttttatg ttgattttga aacccagaag cgttacccaa aagaatcagc ttactggtac
    17881 aagttggtca gtgaaaccaa aacgattatt taataagtga taatggagga agaaaagatg
    17941 aataaagagg aattacaaat gcttggtttt gagatcgttg catattcagg cgacgctcgc
    18001 agtacattat tgaaattatt gaaagaagta agacaaggaa actttgaaca tgttgattcg
    18061 gcgttgaaag aagccgacga aaatttaacc cttgcacata attcgcaaac aaaaatttta
    18121 gcggaagaag catctgggaa agagatggag atgggattca tttttatcca tgggcaggat
    18181 catttaatga ccactttatt gctgagagat ctgattcaag actttattgt actgtacaga
    18241 cagaatcaag gataggagaa gaacgctatg gatggaatca ttaaacaaat cgaaaaaggg
    18301 aaaccatttt ttgaaaaaat ctcacgtaat agttatttag gagccgttcg tgacggattt
    18361 ttaacagcga tgccagctat tttattttca agcattttca ttttgattgc tgcggttcct
    18421 gagatttttg gttaccaatg gcctgaatct gtctccacat ggctatggaa agtctataat
    18481 tattcgatgg gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg cttggctgat
    18541 tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt gatgctggcc
    18601 tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg aaggtggatt ttcaacggaa
    18661 tatatgggga caaagggact actggcttcc tttgtcgctg catttttaac agttaattta
    18721 tataagtttt gtgtgctgaa agatattacc attaaaatgc ctaaagaagt tcctggaacg
    18781 atttcgcaaa ccttccgaga tatttttcct ttctcttttt cagtatttgc ggccgtttta
    18841 attgatttag gcattcgaca aatcttccaa atctcatttg cagaagcaat cattacggtg
    18901 ctgcagccgc tatttacagc agctgacggg tacttaggaa tcgctattat ctggggagcg
    18961 atggcgctgt tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc ggcaattgcg
    19021 gcaattatct atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg tgaacaagct
    19081 tctaaagtac tgacggtggg tttagggaat tttgtaggaa ctatgggagg aactggggca
    19141 acattggttg ttccttttat cttcctactc tttgctaagt ctaagcagtt gaaggcagtt
    19201 gggaaagctt cctttattcc agtaagtttc gctgtcaacg aaccattgct atttgcggca
    19261 cccatgattt tgaaccctta tttttttatt ccatttttgt taacacctgt tgtaaacgtt
    19321 tgcctgttca agttttttgt cgatgtattg ggcatgaaca gctttatgta cgttttgcct
    19381 tgggcaactc cagcaccgat cggtttggtt ctaggaacgg gcatgggtat cttagctttt
    19441 gttctagcag cggttctaat cgtcgttgac ttcctgatct atctaccatt ttgcaaagca
    19501 tacgatgctg tcttagtaga gaaagaagca caaaaagcag aggtgatcgc aaccgaggaa
    19561 ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt tatcagcgat
    19621 gcaaataaag aaatcaatgt gttggtactt tgtgctggcg cagggacaag tgcgatgtta
    19681 gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc aatggcgggt
    19741 gcttatggtt ctcattacga aatgctgcag gattttgata tggtggtctt agcaccgcaa
    19801 gtcaattctt actatgatga tatcaaagaa gacaccgatc gcttaggaat caagctggtt
    19861 gcgacaaaag gagcagaata tatcggatta acgagagatc ctgaaaaagc tgttcagttt
    19921 attctgaacc aatttcaata aaggaggagc cataatgaaa atgggaattt ggttgcgtaa
    19981 agctgtttta tttgcagctg tgttaggggc tttgggggct gtttcaaaaa ctgttgaagc
    20041 atctgccagc gaatttattc gcggagcaga tatttcgatc ctgtcagata tggaaaaatc
    20101 tggtgcaaaa tattatgaag acgatatttc aaaagatgcc ttagagattt taaaaaataa
    20161 tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct tataatagta atggagaaag
    20221 ttatggagca gggacaaatg atctggaaac gacgattgac cttgcaaaac gagcaaaaaa
    20281 tctgggactt aaggtgctgc tggattttca ctacagtgat ttttgggttg atcctgggaa
    20341 acagaatctt cctaaagcat ggcaaggtct gacctttgaa gaaatgaata cggcattgta
    20401 cgactataca aagaacgttc tgtcggaaat gaagcagctg gacgtttatc ctgatatggt
    20461 tcagatcggc aatgaactga atagcggaat gctctggcct tatggcaagt cttggggaga
    20521 aggcggggga gaatttgatc gattggctgc ttttttgaag tctggtatcc aagctgttcg
    20581 agacactcaa cctaaaacaa ctccagtaat gctccactta gctgatggtg gcgatacagg
    20641 agcatttact tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg atttaattgg
    20701 agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa cttcaaacca atatgaatgc
    20761 gatcagccaa cggtatggta aagaagtgat cgtagttgaa acagcttatg ggcatacaac
    20821 agcgaatgcc gatactatgc caaatgcttt tggtgaagcc gaagcagctg cggggggata
    20881 tgaaccaaca ccgacaggac aagcagagta tttgctggat cttgccgatc ggattcaggc
    20941 agtaccgaat aatcgcgggg cgggcttctt ctattgggaa cctctttggt acaacggaaa
    21001 tgtgagttgg gcaacacaag caggtatgaa ttatctcagt gttcaatcca ctatgggaaa
    21061 cgaatgggac aaccaagcga tgtttgattt tgctggaaac gccttgccaa gcttgcgtgc
    21121 gtttaaacaa gcaggggcgc aaataaatct agttaaaaat gcaagtttcg aaagcgatgg
    21181 accaacgacc agtccttcca attggcaaac ctggtttcaa caaggaaccg ctaagggtac
    21241 gatcaaaacg gaagcgggca ctttttttgg ggactacaaa ctaacttttt ggcaggatca
    21301 agcatacgag gcatcagctt atcaaaatgt tagtggcata agcaaaggaa cttacacatt
    21361 gagtgcctat gtaatgtctg ggggagagtt tgatactaat cagttatata tcaaaaatta
    21421 tggtggagcg gaactaaata ctgcggttgt taaaacaagt gaatggacaa aaattacaat
    21481 caaagacatt ccaattacaa caggtgtttg tgaaattggg gtttatagca aggcttcagg
    21541 aaatgcttgg acgaacgttg attttgtttc attggtgaaa caatagactg cggatcgttt
    21601 cgattcaaga tcggaggttc ccttcagcca catcgttagc gtagaaggca ggaatcgaac
    21661 ctgcacctcg cctttgccaa aaataaccag aaacgatttc cagtcatttt taccaaaacg
    21721 agtaaagcac cagacttcat ctacactttg tattatgcta aagagagttc aacgatttcg
    21781 taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt gtttaagaaa
    21841 gggtaacctt agaattccgt cgtgaatatg atttgagcac ttagatggat agaaaaaagt
    21901 tctcggaaaa cagttcgttt ttcgagaact tctttttatg ataagaatga aacaaattaa
    21961 cttatattat agtctctttt ggtagggaac catcttattt ttaatgaaaa tcaagcattt
    22021 tagctattga agaatgttga tttataggga ttccccctcc atattatgtt ggctctttat
    22081 tcccactcca ctgttgctgg tggtttagaa gtgatgtcat acacgatacg gttgacgtgg
    22141 gctacttcgt ttacgatacg tacagagatt ttttgcaaga cttcccatgg gatacgggca
    22201 aagtcagcgg tcattccgtc gattgaagtt acagcacgga taccgactgt gtagtcgtaa
    22261 gtacgtccgt cacccataac accgacagag cggatccctg gaagaacagt gaaatattgc
    22321 cagatatcac ggtcaagtcc agcagctgcg atttcttcac gcaagatagc atcagattcg
    22381 cgtacgattt ctagcttgtc ttcagtgatt tcgccaagaa cacgaatacc aagaccagga
    22441 ccagggaatg gctgacgcca aacgattgaa tcaggcatgc ctaactgagt ccctaattca
    22501 cggacttcgt ctttgaataa tgtgtttaat ggttcgatca attcaaattg catatcttct
    22561 ggcagtccac ctacattatg gtgagatttg atcgtctgtg ctgtttctgt accagattcg
    22621 ataacgtcag tatacaacgt tccttgtgct aggaaagaga cgccttcttc accagcaagt
    22681 tttgttgctt cgtcatcaaa aacatatacg aattcattcc caatgatttt acgtttttgt
    22741 tctgggtcag atacaccttt caacttgtct aagaagcgat ctcttgcatt gactttgatg
    22801 atgttcaagc cgaattttcc gcctagactt tccattactt gttccgcttc gcctttgcgt
    22861 agcagaccgt ggtcaacaaa gatagatgtc aattggtcac cgatcgcttt ttgtaaaaga
    22921 accccaacga cactagaatc aacgccacct gaaagaccaa gcaagacttt tttgtcacca
    22981 acttgttcac ggattttttc tacttgcata tcgataaagt tatccattgt ccagttacct
    23041 tcacagtgac aaacatcaaa ggcaaagtgg cgcagcaatt cgattccgta ttcggaatga
    23101 cgtacttctg catggaattg gataccataa aaattacgtg ttgtgtcttg gatagaagca
    23161 atcggacaat ctttgcttgt tccgactgtt tcaaagcctt caggaacttt agttaccaag
    23221 tcaccatggc tcatccatac tgtttgttgt tttggtgttt ctttaaacaa gacagcttga
    23281 tcgcttgtta cttctaacaa ggctttccca tattcgcggt ttttagcagg ctctacggaa
    23341 ccgccaagtt tgtacgtgat caattgcata ccatagcaaa tccctaatac aggaatacct
    23401 aaattgaaga tctcaggatc gatcccaaat gcattctcat catatacact gtttggtccg
    23461 ccagaaagaa tgattccctt tggattcatc gcttttactt cttctgcagt aatgcggtgg
    23521 ctcaataatt cggaaaagac tccaagatca cgaatacgtc ttgtgatcaa ttggttatac
    23581 tgactaccgt agtctaatac gataatcttt tcaacgttcg tcatatcggc aacgtttgtc
    23641 aaatttatta cccctatccc tttaaatttt atctcaaaat agtcaaacta ttttaaaatg
    23701 ctaatatttt cgcatgaaaa tcgaatcgat gatatgattt tcagttttat ggagaattat
    23761 atcggctctt cctcgtgtag gcaagatata ttcctgtaaa tttttcaaat tcacattttt
    23821 ccatacatct tttgccattt taaaggcttc ttcacgatct ccgatagcaa aacgataata
    23881 ataattctct ggattttgga aagccgtatc cagtaacgca ccaaaacgat ccaaatacca
    23941 tttttcgatc agcacgggat cggcatcgac atagacagaa aaatcaaaaa agtcgctgac
    24001 gtagatttgc tgattcgcag gtaattgaag cgtattgatc ccctcaacga tcaagatatc
    24061 aggctgctgg atcaattcgt acttgtctgg aatgatatca tacacatcat gtgagtaaac
    24121 aggtactttt atctcttcct gaccagattt tacacggttc aagaaatcga tcagcatctc
    24181 catatcataa ctttcaggaa atcctttacg gtccatgatt ccacgctctt ttaatatacg
    24241 atttggataa agaaatccgt cagtcgtgat cagctgaacg tttcttcgtt tgaaggtacg
    24301 agacaaaatc gtctgcagca gtctggctgt tgtacttttt ccaacggcta cacttccggc
    24361 gatcccgata ataaaaggcg gtacttctac gtatttatga agaaataaac ctttactcaa
    24421 gaacaaagat tcaaattctt tcatataaag ataaatcaga tgagcaagag gtacatagat
    24481 atctcgaaca tcctgcatgg aaatgcggtc attcaaactt ttgatattat ctagttcatc
    24541 ttgtgtcaaa ggtgcttgcc cattacgata aaattctcgc cattcttctc ttgccacacg
    24601 gtagtagttc attggatcat tcatattagc ctcctgcgtt ctcccatcaa tggaactatt
    24661 ttaacacaaa ataaacgcaa accccaccac ttaagagaga ttgccgatct ttttgtataa
    24721 tctttataat agaaagaaac ggagtcttat tcttgacttg cgcacggata cataaaagga
    24781 tagactgttg tcatgaaaga aatgagaagg atggaagaca tgacaaatca ttactattct
    24841 gaacgcccag atacggcgca cgattttgaa caatgggctt ttgagttaaa aggcagaacc
    24901 tttcaatttg taacagattc tggtgtattt tcacgtgaaa cagtggatta tggttcacgt
    24961 gtcctgattg atgctttcga ttgggaagag ttacctgatg aaggaaagat cttagatgtg
    25021 ggatgcgggt atggaccgat tgggttagct ttggcatttg cgacacagcg gtttgttgaa
    25081 atggtcgaca ttaatgaacg agcggtaagt ctagcgcaag gaaacgcaaa gcgtaaccaa
    25141 atcgaacatg ttgatatcca tcaatcgaat atctatgaag cagttcatga agaaacctat
    25201 gcagctatcg taagcaatcc gccgatccgt gctggaaaaa aagttgtaca tgaaattttg
    25261 acaggcgcat atcctcggtt gaagaagggc ggtacactga caattgtgat ccagaagaaa
    25321 caaggagcgc cgagtgcgca aaagaaaatg gaagaaacat ttggtaatgc agaaatcgtc
    25381 acaaaagata aaggatatta tatcatcaaa agtgtgaaag aataactagc tgaaaaatag
    25441 tggagcgacg aaagagtgga actgggcgtg catgtacggg tacgtatcag ttcttttttt
    25501 gttgagaagt ttttaaaaac aaagaactat tttttcttca aaaagaaata gtaggaacag
    25561 agtcgttgtt ttttacgata aaataaataa gatacagaac ttgtatagaa ggctgccaag
    25621 tcggtagata aaggaggttt ttatcaaaaa acatactttc cttttttctc tattcttttc
    25681 ttgggggttt tggtacactg atagggaatt tagatagaag ggagccaata ggatgaagct
    25741 gaccatcaga gatatcgcag aaatggcagg cgtatcagta acaactgtct cacagatttt
    25801 gaataacaaa ggaagcagat ttagtgataa gacaagaaaa aaagtgttgt cgatcgtgaa
    25861 tgaacatcat tataagcccg attatttcgc ttcaaatgtg atcaatcgac attcaaagac
    25921 catcggaatg atcgtccctg atgtcactga tttcttcttc tccaaactga tcgaaggtgt
    25981 agagagctat ttgaatcctt tgggctatgt gatcatgtta tgcaattctc gtcatagcca
    26041 agaaaatgag atcaagtatt tacaagagct ttcccaccgc tcagtggacg gaatcttgct
    26101 ggccacacca aatattttac ctgagaaata tgcattagac agcggttttt atcaaaaaat
    26161 gccaatcatt ttaatcgatc gcggattgaa tcgtcgggat aacggacggc ttatcgtaaa
    26221 ggaatatgag ggcgcctatc aagctgtttc gctgtttatt gaaaatggcc atacgaagat
    26281 cggcatgctg aaggaaacca ctggatacta tcaattagaa gaacgtttca acggatatcg
    26341 tcatgcgctg aaagatcatg gcctgccttt taatgggaaa tttgttgaac atggagagtt
    26401 gactgtccaa ggtggatata ctgcatcaaa aaaattactc aaacataaag atatcacggc
    26461 tattttttgc gggaatgatg ccatggcgat cggatgctat caggcgatcg atgaattggg
    26521 caaaaaaatc ccagaagata tctcagtcat aggattcgat ggattaaagc tgtcagaata
    26581 catgattccg caattgacga cagtgcagca gccaagtttt gatattggat tttatgctgc
    26641 acgtttcctg atcgatacga tcgaatttcc tcaaagaaaa gttccgaaca aagtgttcga
    26701 aacaaaatta attattcgtg agagcgtaaa agtattgaca aagggctaac tcggcggtat
    26761 attgcttatg taagcattta caaaggctag ttagtgcttt ttttattttt agtaaaccag
    26821 tttactaaag tggtttacct ttcctaaaaa taatgtataa taccgaatga agacaaggag
    26881 gatcaggcaa tgcgaatggt ggatctgatt gaaaaaaaga gggatggaaa cgagctgtcg
    26941 aaagaggaaa tcgagtacat cgtcacgaac tatacgaatg gaaagattcc agattatcaa
    27001 gtcagtgcat tattgatggc aattttttat caagacatga caaatgaaga aatcacgaac
    27061 ttaacattgg cgattgctaa ttctggtgat gtcatcgatt tgagttctct tgaaggaata
    27121 aaggtagata agcattcgac aggtggcgtt ggtgacacga ccacattgat tttagccccc
    27181 ctagtagcta gtgtcggagt gacagtggct aaaatgtctg gacgcggtct aggttacaca
    27241 ggtggtacac tagacaagct agaagcaatt cccggatttc agatcgagct ttcggatgaa
    27301 gaatttgtac gcattgttaa tgaaagtaaa gtagcggtaa ttggtcaatc aggaaacttg
    27361 gcaccggcgg acaaaaaatt gtatgccttg cgtgacgtca cagcgaccgt tgattctctt
    27421 ccgctgattg ccagttcgat tatgagtaaa aaaatcgcgg caggagcaga tgcaatcgtt
    27481 ttggatgtga ccactggaga cggtgctttc atgaagaaca tcgaagatgc aagacgtctg
    27541 gcaaagacca tgacgagcat tgggaaactg gctaaccgag aaacagtggc ggttatctca
    27601 gatatgtcag aacctcttgg cgaagctatc ggaaatagtc tagaagttgt cgaggcaatc
    27661 gaaacattac aaggaaatgg tccagaagat ctagtagaaa tgtgctacgc cttaggaagc
    27721 caaatggttg ttcttgctgg caaagctaag acaaccgatg aagcaagaac gttgcttcaa
    27781 gaagctctgg aatcaggtaa agcgttagca aaattcaaag aaatgatcca aaatcaaggg
    27841 ggagacccag cgatcgtgga acatccagaa cgaattttga ctgcacgcta cacaatggag
    27901 cttcctgcta aacaaagcgg agttgtgtca aaaatcgtgg caaatgaatt aggaattgct
    27961 gcaatgatgc taggtgctgg gcgtaaaacc aaagaagaag atattgatca cgctgttgga
    28021 ttgaaactgc ataaaaaaat tggtgacact gtgacaaaag gtgagtcact attgacgatc
    28081 tatagtaacg acgaagaaat cacttctgtc attgaattat tatataaaaa tattgaaatt
    28141 ggtgaatcgg caatgaagcc aacgttgatt catgacatca tcacagaata aaggaaaaaa
    28201 gcggacttta ccgttttaaa gaaaagggga aaagaaatgg aactaaaccg aatgatcgac
    28261 catactattt tgaaagcaga cgcatctaaa gaagctgtga tgcaaattat cgaagaagca
    28321 aaaaaatacc atttttattc tgtctgcatc aatccagcat gggttgcgct agcgaaagaa
    28381 caattacaag gtgagccagt agccgtatgt acagtaatcg ggtttcctct aggagcaaac
    28441 acacccgaga caaaagcatt tgaaacaaca gatgccatca ataacggtgc agatgaagtc
    28501 gatatggtca tcaatatcgg tgctttgaaa tcaaaggatg atcaacaagt ccaaaaagat
    28561 atcgaagcag ttgttgaagc agcaaaagat cgtgcgcttg tcaaagttat tattgaaaca
    28621 tcgttattag atagagaaga aatcatccga gcttgcgaaa tagcaaaagc tgcaggcgct
    28681 gattttgtca aaacatcaac tggtttctca acaggcggag caaaggtaga ggatgtccgt
    28741 ttgatgcgtg aaactgtagg accagaaatg ggtgtcaaag cttccggcgg tatccataac
    28801 gaagaagaag caatggcaat gatcgaagct ggtgctacaa gaatcggaac aagcgcagga
    28861 gtagcaatcg tatctggttc aactggtgaa ggctattaat tttttcaaag gagaagctca
    28921 tggcagaact caaacaagaa tggatcaaca cagctattga agcgttagat aaagcctatg
    28981 ttccttattc ccattttccg gtaggcgctt gtctcgtaac ggaatcaggc aagatttatc
    29041 aaggcatcaa cattgagaat gcatcatttg gattaacgaa ttgtgccgag cgcacagctt
    29101 ttttcaaagc agtatctgaa ggagaaaaaa catttacgca tctagtcgta gcggggcaca
    29161 cccccgatcc tatctctcct tgtggtgcat gtcgacaagt aatggcagaa ttctgttccc
    29221 ctgatatgcc ggttacgcta gtaggagaca atggtgtgac gaaggcaacg accgttcgtg
    29281 cactgctgcc ttatgcattt actgaaaaag atctataact catgttcatt cagtcagctg
    29341 actgttgaaa aataaaacaa ttattttagg aggctttaca gaaatgaaaa aagcaaaatt
    29401 atttggttta ggtgctgtcg cactagcagc agggttattt ttaggtgcat gcggcaacaa
    29461 cggctctact gattcatccg gtggcaaaag ctcatcagac acaacaactg cagcattgat
    29521 cacagacaca ggtggtgtag acgaccgttc attcaaccag tcagcttggg aaggactaga
    29581 aaaatggggg aaagatcaag gactttcacg aggaaatgat ggcttccaat atttccaatc
    29641 ttcaaatgaa tcagattaca tcccaaatat tgaccaagca ttgaatgctg gattcaaaac
    29701 gatttttggt attggttata aactaaaacc agcgatccaa gaacaagcaa caaacaatac
    29761 aggtacgaac tttgttatta tcgatgatgt catcgatgga ttagataacg ttgtatctgc
    29821 aactttcaaa gacaacgaag cttcttatct tgcaggtgtt gcagcagctt atacgactga
    29881 aacaaacgta gtcggcttta tcggcggtgt aaaaggtgaa gtaatcgatc gttttgatgc
    29941 tggattcaaa gccggtgttg atgcaggtgc gaaagaatta ggaaaagaaa tcaaagtatt
    30001 gaatcaatat gctggtgact tctctgctcc agataaagga cgttcaattg ctcaaggtat
    30061 gtatgcacaa aacgcagata tcatcttcca tgcttcaggc ggtacaggta acggtgtatt
    30121 ccaagaagca aaatcattga acgaatcagg cgacaaaaaa gtttgggtta tcggtgttga
    30181 ccgcgaccaa tcagacgaag gcgaatacac attaaacggc gaaaagaaaa acttcacatt
    30241 gacatctaca ctgaaagcag ttggaacagt agtagaagat ttggcacaaa aatcagcgga
    30301 cggtaaattc cctggcggcg aacacactgt ttatggattg aaagaagacg gtgttggtct
    30361 aacagaagga caactttctg acgaagcgaa aaaagcagtt gatgaagcaa aagaaaaaat
    30421 catttcaggt gacgtaaaag ttccagaaac tcctgaagaa aactaaaacc atttctatag
    30481 ccttgttaga cacatgtttg acaaggctat ggtttgatta gaacaagaat tgttcattta
    30541 tccaagctgc cttgtgacag tttggataag ggaacaactc cctgaagtaa tgtttgtttc
    30601 aaaagaagaa cgctcactaa gcgtgaatct tagtcaatcg ttgccctaga tagaaatcgt
    30661 taggatgtga aaaaaaggtg gttcaagaaa atattgtcat agaaatgcag aatattacca
    30721 aagaatttgg aacattcaag gcaaatgaca acatcaattt acaagtaaag gcaggagaaa
    30781 ttcatgcgtt gcttggagaa aacggtgctg gcaaatctac attgatgaac gtgctttccg
    30841 gattattaga gccgacatca gggaaaattt tgatgcgtgg gaaagaagta aagatcacaa
    30901 gtccgaccaa agccaaccaa ttagggattg ggatggtcca tcagcacttt atgcttgttg
    30961 atgcctttac tgtaacagaa aacatcgtgt tgggaagcga gcctagtcgc gcagggatgc
    31021 ttgaccataa aaaagcacga aaagagatcc aaaaagtttc tgaacagtat ggattgtcag
    31081 tcaacccaga tgcttacgtt cgtgatattt cagttggaat ggaacaacgg gtagaaattt
    31141 taaaaacact ttaccgagga gcagatgtac tgatttttga tgagccgaca gctgtattga
    31201 cccctcaaga aattgatgaa ttaatcgtga tcatgaagga attagtcaaa gaaggcaagt
    31261 cgatcatttt gattacgcat aagttagatg aaatcaaagc agtagctgac cgctgtacag
    31321 ttatccgccg tggaaaagga atcggtacag tcaacgttaa agacgtaacc tcacaacaat
    31381 tagccgatat gatggttgga agaacggttt cattcaaaac gatgaaaaaa gaagcgaagc
    31441 ctcaagaagt cgttttgtct attgaaaatc tagtggtgaa agaaaatcgt ggattagaag
    31501 ccgtgaaaaa cttgaactta gaggttcgtg ctggcgaagt acttggtatc gctggaatcg
    31561 atggaaacgg gcagtcggag ttgatccaag ctttgactgg tttgcgaaag gcagaaagcg
    31621 gacatatcaa gctaaaaggg gaagacatca ctaataaaaa accccgaaaa atcactgaac
    31681 atggtgtagg acacgtgcca gaagaccgtc ataaatacgg gttagtccta gatatgacat
    31741 tatctgaaaa tatcgcacta caaacctatc atcaaaaacc ttacagtaaa aacggcatgc
    31801 tgaattattc agtaatgaat gaacatgcca gagaattgat cgaagaatat gatgttcgaa
    31861 caacgaatga acttgttccc gcaaaagctt tatcaggcgg aaatcagcaa aaagcaatta
    31921 tcgctcgtga agtcgaccga gatcctgatc tgttgattgt tgcaaatcca actcgtggac
    31981 tggatgtagg tgcgatcgaa tttattcata aacgtctgat cgaacaaagg gacaaataca
    32041 aagcagtgtt actgatcagt ttcgaattag aagaaatttt aaatgtttcg gatcgtattg
    32101 ctgttatcca tgaaggagaa attgtcggta tcgttgatcc gaaagaaaca tctgaaaatg
    32161 aattaggtct attgatggcg ggctatacct tagaagaagc acgaaaagag ttagaaaagg
    32221 gggctggcgc acatgaatga tcgcagcgaa aaattccgta atttattagt gcctgtactt
    32281 tcagttgttt taggattgat cttaggcgcg atattgatgg ctgcttttgg ttttcagccc
    32341 atccaaggtt attcttctat gctagatgca gcccttggtt cgcaaagagg gataggtgaa
    32401 acgttaaggc aagcaacgcc gttgattttt acagcattag gtttttctgt agctaactca
    32461 gctgggtttt tcaatattgg gctttctgga caagcacttt gcgggtggat cgtcagtgtc
    32521 tgggtggcac ttgcgtttcc agatctgcca aaagttgttt tgcttccgtt atgcgtgatc
    32581 ttaggtgcct tagctggtgc gatggcagcg gctgttccag gtttacttcg agcatttttt
    32641 ggaacaagtg aagtgatcgt aacaatcatg atgaactata tcttattata cgtaagcaca
    32701 tttgctttac atgaagtgat gcctgaaagc tatcgctcaa gtttggactc ttccaatatg
    32761 atcagtcaaa atgcctcact gcggttacca tggttaacac aagcttttgg cggctctcgc
    32821 gtaaactcag gattattttt agcaatcatc gcgcttattt tcgtttggct gctgatgaag
    32881 aaaacgactt taggatttga aatccgttct gttggattga atccttttgc ttctgagtat
    32941 gcaggaatga gtagtaaacg tacgatcgtt ttgtcgatgg tcatttctgg cgctttggca
    33001 gggcttggcg gtgtggttga aggattagga acgtatcaaa acttctttgt tcaaacgact
    33061 tcgctatcta ttggatttga cggaatggct gtttccttgc ttggttctgg atcagcaatc
    33121 ggtattcttt tatcagcgat cttgttcagt attttgaaga tcggtggact tggcatgcag
    33181 acaggagcag gtgttccgtt tgagatcgtc aatgtatcga ttgctttgat tatcttcttt
    33241 gtcgggatca acttcctgat tcgtttcgtc atggctaaag tcttccaagg gaaaaaacaa
    33301 gaagagatcg ttgcgacaat agaatcgaaa ccaacgaatc aaaaccatga agggggcgaa
    33361 ctataatgtc gactatcgaa ttaattgcct tgatccttac ttctacactg gtttattcaa
    33421 caccgctaat tctaacgtca ctaggcggaa ctttttctga acgaagcggg atcgtaaacg
    33481 tagggctaga gggaatcatg gtcatgggtg cttttagttc catcgttttc aacttgacga
    33541 tgagtccatc acttggttct atgacaccat ggatcgctat tttagtgggt ggcgtcgtag
    33601 gtatcctatt ttctctgatt cacgcagtag caacagtcag tttgcgtgct gaccatatta
    33661 tcagcgggac agtaattaat ctgatggcac ctgctttagg tgttttcttg atcaaagcat
    33721 ggtatggtaa aggtcaaact gataacatca cgcagaatct ggggtatttt tctttcccag
    33781 gattagcaga tattccggtc attggaccaa ttttctttaa aaatacattc ttacctgctt
    33841 attgtgctat tttgattgct gtcctagctt gggtgatcat cttcaagacg aaattcggct
    33901 tgcgcctccg ttcagttggt gaaaatcctc aagctgccga cacattaggt atcaatgtat
    33961 atgctatgcg ttattcaggc gtactgattt ctggcttgtt aggcggtatg ggcggtgctg
    34021 tttttgctca gtcgatttcc ggtaactttt cagctggtac gatcgttgga caagggttca
    34081 tctccatggc tgctatgatc tttggtaagt ggaatccttt aggggcaatg ggtgcctcat
    34141 tgttctttgg ttttgctcaa agcttgagta tcattggcgc acaattacca gttatttctt
    34201 cgattccgcc agtattgctg caaattgctc catatttgct gacaatcatt gtgctggttg
    34261 ttttcttagg gaaagcttct ggtccaaaag caaacgggaa gaactatatc aaatcaaaat
    34321 aatcttttag cgaataaaaa gggtgggtta cgggaagtgt ttttcgtctc gccctttttg
    34381 ttttaaaggc aataaaaata aaaagaaaga aggaataatc atgtttaaac gcgtacatct
    34441 gatcgtcatg gattcagtag gaattggaga agcaccagat gccgataagt tcggtgatgt
    34501 tggttctgat acattaggac acattgcgaa agaagcagga ttgaacgttc cacatttaga
    34561 gcagcttggt ttaggaacga tccgtcctct tgaaggtgtg gaatcagtga aagatcatca
    34621 aggctatgcg acgaaattag aagaagtttc tgtagggaaa gatacgatga caggccactg
    34681 ggaaatcatg ggattgaata tcaaaactcc atttcgtgtg tttcctaatg gattccctga
    34741 tgatctgatc aaacaaatcg aagaattttc tggccgtaaa gtcgtatgta acaaacctta
    34801 tagcggaaca gctgttattg acgattacgg tgaacaccaa atgaaaacag gcgatttaat
    34861 tgtatatact tctgccgatc ctgtccttca gatcgcggca catgaagaaa tcattccttt
    34921 agaagaatta tatcggattt gccaattcgt tcgtgatata acaaaagatg atccgtatat
    34981 gattggccga atcattgcac gtccgtacgt gggtgaacca ggagacttca cacgaacaag
    35041 caatcgccat gattatgcgt tgaatccatt cggacacaca gtccttgatt ctttgaaaga
    35101 agcaggaaag gacgtgattg ctgttggaaa gatcaacgat attttcaacg gacaaggaat
    35161 cactgaatct gtccgcacga aaagcaatat ggatggcgtt gaccagctgc tgaaagttat
    35221 gaaaaacgat ttttcaggaa tcagctttac caatttagta gattttgatg cattatacgg
    35281 tcatcgtcgg gatgtagtcg gctatgcgca tgcgattgaa gaatttgact tacgaattcc
    35341 agaattgctt gaagcgatgg aagaagacga cttgctaatg attacagccg atcacggaaa
    35401 cgatccgacg tttactggta ccgatcatac gcgagaatat gtaccgttat tggtttacag
    35461 taaaaaaatg aaagaacaag gggcattgcc tcaaggatat tattcagata tttcagcaac
    35521 gattgctgag aactttagtg tttcttcgac tgaaaatggc gaaagcttct tgaaattatt
    35581 aaaataatct ttttaaatag gagaaagcaa atgaccaagt taagcgagat gttacaagaa
    35641 acgacccagt ttatccttga taaaggagta aaagaaattg attttggttt gattctaggt
    35701 tcaggattag gtgaattagc agatgaaatc gaagatgctg taatgatttc ttatgatgag
    35761 atcccgttct ttcctgtttc gacagtagtt gggcatgcag gaaagctagt ttatgggtct
    35821 ctggcaggca agaaagtgtt agccatgcaa gggagattcc actactatga aggtcattcc
    35881 atgcaaacag tgacctatcc agtacgtgtg atggccggac taggggccca ttcagtcatt
    35941 gtgactaatg catgtggtgg tgtcaacgaa tcatttgtac caggagattt gatgctgatt
    36001 accgatcata tcaattttac tggtgaaaat cctttgatcg gtccgaatga agatgcaatg
    36061 ggccctagat ttccagatat gagtcaagct tatacgcctg actatcaagc aacagcaaaa
    36121 gcagcagcag aaaaactagg tttggatctt aaagaaggcg tatatatggg ctattctgga
    36181 ccaacttatg aaacgcctgc ggagatccga atgtctcgga caataggagc cgatgcagta
    36241 gggatgtcga ctgttccaga agtcatcgta gcagctcaca gcggattaaa agtgttagga
    36301 atctcttgta ttaccaactt agcagcaggt atgcaagcga atctgagcca tgaagaagtt
    36361 gtagaaacaa ctcagagagt caaacaatct tttaaagcat tggttaaaga aacattgaca
    36421 ttattgtaat aggaggaaca ataaaatgag tgtgcatatt gaagcgaaag aaggcgagat
    36481 cgccgataag attttacttc cgggagatcc tttgcgagct aaatatattg cggagacttt
    36541 tttggaagat ccagtttgtt acaatcaggt acgcggtatg ttaggcttta ccggcaaata
    36601 taaaggagaa cgtatttctg ttcaaggaac aggaatggga atgccatcag ccaccatcta
    36661 tgcccatgaa ttgatccagt cgtatggtgt aaagaaattg attcgagtag gtacatgcgg
    36721 tgcattgtca aaagacgtcc atgttcgtga tttagtacta gcacaaggtg cagcgaccag
    36781 ttcttcgatg atcgaaaaaa atttccaagc ctttcatttt cctccaatca gtgatttcaa
    36841 tctattactg aaagcatatg agatagctaa agaaaagggc tatacggttc atgtaggaaa
    36901 tgttctttcg gaagattctt tctacaaaga tgatttgaca gagacgttcc aattagctga
    36961 acttggcgtt ttaggagtgg aaatggaagc agcggctctt tattacttag gcgcgaaata
    37021 ccatgtgcag acattaagtt tgatgaccgt tagcgaccat ttgatcaccg gtgaagaaac
    37081 aacagctgca gaacgtcaat ctacgttcaa tgaaatgatc gaagtaggat tggaaacagc
    37141 gatagcagaa taagaaggaa atgaaaaagc tgccctgcat taagtaataa gaacatccaa
    37201 gcaaaaatag ctcctcatgt ttttgacttg gctggactta ttattgcaaa tgtaagcttt
    37261 tgaacaccgt ttaccaagag gttgtgaa
//
LOCUS       DACEPD010000017        35438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.017, whole genome shotgun sequence.
ACCESSION   DACEPD010000017 DACEPD010000000
VERSION     DACEPD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.017"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1152
                     /locus_tag="IW797_000864"
     CDS             <1..1152
                     /locus_tag="IW797_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223656.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IW797_000865"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IW797_000865"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ1223657.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IW797_000866"
     CDS             2728..3003
                     /locus_tag="IW797_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ1223658.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IW797_000867"
     CDS             3009..3776
                     /locus_tag="IW797_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ1223659.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IW797_000868"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IW797_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ1223660.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IW797_000869"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IW797_000869"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ1223661.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IW797_000870"
     CDS             7920..8222
                     /locus_tag="IW797_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ1223662.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IW797_000871"
     CDS             8225..9388
                     /locus_tag="IW797_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ1223663.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IW797_000872"
     CDS             9414..12842
                     /locus_tag="IW797_000872"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ1223664.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IW797_000873"
     CDS             12863..13987
                     /locus_tag="IW797_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223665.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IW797_000874"
     CDS             13977..14414
                     /locus_tag="IW797_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ1223666.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IW797_000875"
     CDS             14398..14736
                     /locus_tag="IW797_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ1223667.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IW797_000876"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IW797_000876"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ1223668.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IW797_000877"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IW797_000877"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ1223669.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IW797_000878"
     CDS             15893..16936
                     /locus_tag="IW797_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1223670.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IW797_000879"
     CDS             17095..17823
                     /locus_tag="IW797_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ1223671.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IW797_000880"
     CDS             17939..18184
                     /locus_tag="IW797_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1223672.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IW797_000881"
     CDS             18181..18399
                     /locus_tag="IW797_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ1223673.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IW797_000882"
     CDS             18535..19074
                     /locus_tag="IW797_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ1223674.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="IW797_000883"
     CDS             19067..21340
                     /locus_tag="IW797_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ1223675.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="IW797_000884"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="IW797_000884"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ1223676.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="IW797_000885"
     CDS             complement(22513..22767)
                     /locus_tag="IW797_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ1223677.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="IW797_000886"
     CDS             complement(23167..23436)
                     /locus_tag="IW797_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223678.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="IW797_000887"
     CDS             complement(23906..24361)
                     /locus_tag="IW797_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ1223679.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="IW797_000888"
     CDS             complement(24499..26427)
                     /locus_tag="IW797_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ1223680.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="IW797_000889"
     CDS             complement(26624..27457)
                     /locus_tag="IW797_000889"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ1223681.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="IW797_000890"
     CDS             complement(27649..28353)
                     /locus_tag="IW797_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1223682.1"
                     /translation="MTETTLYIVRHGKPMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="IW797_000891"
     CDS             complement(28376..29002)
                     /locus_tag="IW797_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1223683.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="IW797_000892"
     CDS             29328..31703
                     /locus_tag="IW797_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ1223684.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="IW797_000893"
     CDS             complement(31727..32218)
                     /locus_tag="IW797_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ1223685.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="IW797_000894"
     CDS             32405..33325
                     /locus_tag="IW797_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1223686.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="IW797_000895"
     CDS             33479..33823
                     /locus_tag="IW797_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ1223687.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="IW797_000896"
     CDS             complement(34013..34513)
                     /locus_tag="IW797_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1223688.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="IW797_000897"
     tRNA            35041..35124
                     /locus_tag="IW797_000897"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
BASE COUNT        11775 a         6169 c         7297 g        10197 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag gcgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgttg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcggttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaa
//
LOCUS       DACEPD010000018        34522 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.018, whole genome shotgun sequence.
ACCESSION   DACEPD010000018 DACEPD010000000
VERSION     DACEPD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.018"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(133..306)
                     /locus_tag="IW797_000898"
     CDS             complement(133..306)
                     /locus_tag="IW797_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1223689.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="IW797_000899"
                     /pseudo
     CDS             754..3116
                     /locus_tag="IW797_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="IW797_000900"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="IW797_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ1223690.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="IW797_000901"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="IW797_000901"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ1223691.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="IW797_000902"
     CDS             5704..6489
                     /locus_tag="IW797_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1223692.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="IW797_000903"
     CDS             6502..7353
                     /locus_tag="IW797_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ1223693.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="IW797_000904"
     CDS             complement(7477..7929)
                     /locus_tag="IW797_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1223694.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="IW797_000905"
     CDS             complement(8394..8762)
                     /locus_tag="IW797_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ1223695.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="IW797_000906"
     CDS             8912..9394
                     /locus_tag="IW797_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ1223696.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="IW797_000907"
     CDS             9431..10441
                     /locus_tag="IW797_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ1223697.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(10556..11269)
                     /locus_tag="IW797_000908"
     CDS             complement(10556..11269)
                     /locus_tag="IW797_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223698.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(11459..12049)
                     /locus_tag="IW797_000909"
     CDS             complement(11459..12049)
                     /locus_tag="IW797_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ1223699.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            12533..12760
                     /locus_tag="IW797_000910"
     CDS             12533..12760
                     /locus_tag="IW797_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ1223700.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            12776..13942
                     /locus_tag="IW797_000911"
     CDS             12776..13942
                     /locus_tag="IW797_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ1223701.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            14176..15219
                     /gene="hrcA"
                     /locus_tag="IW797_000912"
     CDS             14176..15219
                     /gene="hrcA"
                     /locus_tag="IW797_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ1223702.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            15313..15876
                     /gene="grpE"
                     /locus_tag="IW797_000913"
     CDS             15313..15876
                     /gene="grpE"
                     /locus_tag="IW797_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ1223703.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            15927..17756
                     /gene="dnaK"
                     /locus_tag="IW797_000914"
     CDS             15927..17756
                     /gene="dnaK"
                     /locus_tag="IW797_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ1223704.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            17907..19073
                     /gene="dnaJ"
                     /locus_tag="IW797_000915"
     CDS             17907..19073
                     /gene="dnaJ"
                     /locus_tag="IW797_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ1223705.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(19155..19820)
                     /locus_tag="IW797_000916"
     CDS             complement(19155..19820)
                     /locus_tag="IW797_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ1223706.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            19796..19969
                     /locus_tag="IW797_000917"
     CDS             19796..19969
                     /locus_tag="IW797_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1223707.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(20014..20751)
                     /locus_tag="IW797_000918"
     CDS             complement(20014..20751)
                     /locus_tag="IW797_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1223708.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(20753..22255)
                     /locus_tag="IW797_000919"
     CDS             complement(20753..22255)
                     /locus_tag="IW797_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1223709.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            22514..24508
                     /gene="uvrB"
                     /locus_tag="IW797_000920"
     CDS             22514..24508
                     /gene="uvrB"
                     /locus_tag="IW797_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ1223710.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            24518..27334
                     /gene="uvrA"
                     /locus_tag="IW797_000921"
     CDS             24518..27334
                     /gene="uvrA"
                     /locus_tag="IW797_000921"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ1223711.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            27597..28862
                     /locus_tag="IW797_000922"
     CDS             27597..28862
                     /locus_tag="IW797_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ1223712.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            28866..29597
                     /locus_tag="IW797_000923"
     CDS             28866..29597
                     /locus_tag="IW797_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ1223713.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            29732..30616
                     /gene="rapZ"
                     /locus_tag="IW797_000924"
     CDS             29732..30616
                     /gene="rapZ"
                     /locus_tag="IW797_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ1223714.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            30613..31611
                     /locus_tag="IW797_000925"
     CDS             30613..31611
                     /locus_tag="IW797_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ1223715.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            31645..32580
                     /gene="whiA"
                     /locus_tag="IW797_000926"
     CDS             31645..32580
                     /gene="whiA"
                     /locus_tag="IW797_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ1223716.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            33123..33773
                     /locus_tag="IW797_000927"
     CDS             33123..33773
                     /locus_tag="IW797_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223717.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            33960..>34522
                     /locus_tag="IW797_000928"
     CDS             33960..>34522
                     /locus_tag="IW797_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ1223718.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        11453 a         5832 c         7324 g         9913 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat aacatattag aaagtctaat
    10561 tatctataac tgtcacatcg attaactgtt tcacgtcatt gggatttcct gtaggaatag
    10621 ctactacaga tgcattatca atgatttctt gagttgtttc tctaatgaac gctggataag
    10681 atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt
    10741 catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca
    10801 catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat
    10861 aatgaataac atgtaattcg aaatcacccg atgtatctct aaaagtatta cctctaatag
    10921 ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg
    10981 actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt gtctcaactg
    11041 tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat
    11101 tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga
    11161 ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc
    11221 ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct
    11281 cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt
    11341 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
    11401 tcatcagtaa aattacttgt cacactattg ataaggagta tttaattgaa gagaatgatt
    11461 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
    11521 tcgttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
    11581 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
    11641 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
    11701 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
    11761 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
    11821 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
    11881 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
    11941 tagaagcagt tttgttttgc catttctcat tttggtttag tgtgctagta atataagcac
    12001 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
    12061 cctttactat ctataacaag gacaacagtt ttaacctaca gacattcatt aaaattgggt
    12121 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagata gtcaacttaa
    12181 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
    12241 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tattaaaaga
    12301 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
    12361 tgaaaacaac cctagagata tataactagg taaccagttt aatgaaagaa aaaatcatcc
    12421 aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt agtatgcgta
    12481 tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat
    12541 agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg cgattaccgt
    12601 ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag catttttaga
    12661 gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc ttgaacgcga
    12721 ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat
    12781 agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc cattttgcga
    12841 gcatatctgc tattactgtg attttaataa agttttttta gaagggcagc cagtagatga
    12901 gtatgtggaa atgctgctga atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa
    12961 tttagaaaca ctttacgtag ggggcgggac accaacttct ttatctgcta aacaacttga
    13021 tcgactgtta tcaggtgcta gagagatcct gccttttcaa aatggtaatg agtttactgt
    13081 agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt
    13141 caatcgttta tccatgggag tgcagacatt tgataatcgc ttattgaaaa aaattggacg
    13201 taaacacacc gcagaagatg tgtatcaaac tatgcaattt ttagaagcag aaaattttac
    13261 caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag gatatctgga
    13321 tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact cattgatcct
    13381 cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac tgcctgatca
    13441 ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc atggacgtca
    13501 ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata acctcatgta
    13561 ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc ttggagatat
    13621 tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag caaatgaacg
    13681 cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag aaatgttctt
    13741 aggactaaga aaaaaaacag ggattactaa aaaacatttc tatgatcgct atcaaaaaac
    13801 aattgaatcg gtgtacggtg atgtgctaag ggaattagag caagaagggt tacttgttaa
    13861 tgaagaagac cgtgtttatt tgacaccaag agggaccttt ttggggaaca atgtgtttga
    13921 gcgctttttg tttgaaaaat agtttaaaga atatttgttt tttatctgaa tttaaataaa
    13981 aaaagttcaa aaatacaata aaggactatt gaaacgttga tataactgga tttacatcaa
    14041 ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca
    14101 ggagaagggc ttcttgctat atttataagt ggattagcac ttagaatata tgagtgctaa
    14161 tgagaggtga ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa
    14221 cactatacga gtagtggcgt gcccgttggt tccaaaacat tgatgtctga gggagtcaag
    14281 gccagctcag caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg
    14341 aagactcact cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat
    14401 catttgttaa aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta
    14461 ggcagagaat ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag
    14521 ctgacaagct atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc
    14581 tttcgtatcg tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg
    14641 aatgtagaaa gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa
    14701 atggtacaga ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa
    14761 cttcgtacgg agattccgat gattcttcat aaatatttcc aaacgacaga agggatgtct
    14821 cagttatttg acaccgttct tggtcatgtt tttgaagata aggtttatgt cagtggacaa
    14881 atgaatctct tggattatga gccccgtcaa gatcttgatc agttcaaatc gatgttttct
    14941 tttatgaaag actcagacgg acttacacaa atgatcactc cgatggatag caatattcat
    15001 atcaagattg gtgctgaatt aggcaatgat ttattgcaga atatgagtat gattcaagca
    15061 agttacgaga tagatggtca tggaagaggt acaatagcct tgttagggcc tactagtatg
    15121 ccttattctc ggatgttcgg tctagtcgat gtttatcgaa gagaactagc agctaaactt
    15181 gctgattact accgttcgct tgacttgtca aactcgtaat gtgaataacg aagctatttg
    15241 cagctgatcg ttctctcttt cttagtaaga gcaatcaaac agctgactga ataatgaaag
    15301 gaagactgta tcgtgaaaga aaatcaagaa gaactagata aagaaatgac agatgctcag
    15361 ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg gtatttcaga agctgaagcg
    15421 gaagaattcg aaacagcgaa attaaaagct gagttagaag aaatggaaga taaatacttg
    15481 cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg tgagcaatta
    15541 caaaaatacc gttcacaaga tttagccaaa aaattacttc catcaattga caacctggaa
    15601 cgtgcattag ccacagaagt gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa
    15661 atggtcttgg aaagtttgcg taacgcactg gaagaagaag gaattgaaaa aatccctgct
    15721 aaaggagaag cctttgatcc taatcttcat caagcagtac aaactgttcc agcgactgaa
    15781 gatacaccag ccgatacgat tgtagaagta ctacaagaag gctacaaatt acatgatcgc
    15841 gtgttaagac caaccatggt cattgttgca caataattgt gcggatataa aacttacact
    15901 ttcaattaaa gaaggagacg ttaattatga gtaaaattat cggtattgac ttaggtacga
    15961 ctaactctgc agtagcagta ttggaaggaa acgaagcaaa aatcatcgct aacccagaag
    16021 gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag
    16081 ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc atcaatcaaa cgccatatcg
    16141 gtgaagcagg ttacaaagta gatgtagaag gtaaatcata tacaccacaa gaaatctcag
    16201 caatgatcct tcaatatatc aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa
    16261 aagcagttat cactgtgcca gcttacttta atgatgcaca acgtcaagca acaaaagatg
    16321 ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc
    16381 ttgcctatgg tttagataaa acagatcgcg acgaaaaaat cttagtattc gaccttggtg
    16441 gcggtacttt tgacgtatct atccttgaac taggagacgg tgtctttgat gtattgtcaa
    16501 cagctggcga caaccatcta ggtggggacg actttgataa caaaatcatc gactacatgg
    16561 tagcagaatt caaaaaagaa aacagcattg acttgtctca agacaaaatg gctttacaac
    16621 gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca agcacacaaa
    16681 tcagcttgcc atttatcaca gcaggagaat ctggaccatt gcatttggaa atgacattga
    16741 cacgtgcaaa atttgatgaa ttaacagctg acttagtaga acgtacaaaa gtaccagtac
    16801 gtcaagcgct aaaagatgca ggtttatcac aatcagaaat cgacgaagtt attttagtcg
    16861 gtggatctac tcgtatccct gcagttgtag aagcagtacg taaagaaaca ggaaaagaac
    16921 caaacaaatc agttaaccct gatgaagtag ttgctatggg tgctgctatc caaggtggcg
    16981 tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt tacaccattg tcattaggta
    17041 tcgaaacaat gggtggagtg ttcactaaat tgattgaccg caatacaacg attccaacaa
    17101 gtaaatcaca agtattctca actgcggcag ataatcaacc agcagtagac atccatgtat
    17161 tacaaggtga acgcccaatg gctgctgaca acaaaactct aggaagattc caattgacag
    17221 atattccagc agctcctcgt ggtattccac aaatcgaagt aacattcgat attgataaaa
    17281 acgggattgt aaatgtatct gcgaaagatc taggtacaca aaaagaacaa aaaatcacga
    17341 tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg tatggtaaaa gatgcagaag
    17401 caaatgcaga agcagataaa gctcgtaaag aggaagttga cctacgtaat gatgtagatg
    17461 cattgctatt ctctgtggac aaaacattga aagaattaga aggcaaagtg gacgaagaag
    17521 aagtgaaaaa agcagaaaca gctcgcgatg aattgaaagc cgctgttgaa gcaaacaaca
    17581 tcgaagaaat gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta
    17641 aactatacga acaagcggca caacaacaag ctcaagaaaa cccagaagca gctcaaggcg
    17701 gagcagatga tgtcgttgat gctgattttg aagaagtcga tggcgacgat aaataatcag
    17761 ttcactgaat ttcgagagaa ggccaaagtt gctactttgg ctttttcttt tgaaaaatag
    17821 tttgaagtat cggtttttgt ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat
    17881 gatgagaacc aataggagga aagcctatgg caacaaaacg tgattattat gaagtcttag
    17941 ggctctcaaa aggagcatca gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa
    18001 aataccatcc tgacatcaat aaagaacctg atgcggaaga aaaattcaaa gaagtatcag
    18061 aagcatatga gattttaagt gacccgcaaa aacgcgctgc atatgaccaa tacgggcatg
    18121 caggcacaga tccaaattac ggtgctggtg gagctggtgg cggatttggt ggattcggcg
    18181 gcttctctgg tggcggcttc ggtggttttg aagatatctt cgactctttc tttggaggag
    18241 gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg
    18301 acttgtcttt tgaagaagcg atttttggtg tagaaaaaga aatcaagtat aatcgggaag
    18361 aaatctgtca tacttgtggt gggaacggtg caaaaccagg cacacaacca actacttgtc
    18421 ataaatgtca tggtgcaggt acgattaatg tagaacgcca aacaccactt ggacgagtga
    18481 tgagccgtca aacttgtgat gtttgtcatg ggacagggaa agaaatcaaa gaaccttgtc
    18541 caacctgtca tggctcagga catgagaaga aagcacattc agttaaagtg aatgtaccag
    18601 ctggtgtaga agaaggacaa caaatgcgtt tagctggtca aggagaagct ggagaaaacg
    18661 gcggaccatt tggtgatttg tatgtggtct tccgtgtaga agaaagtgat atctttgatc
    18721 gtgaaggttc tgaaatttac tatgaattgc ctttaagttt tgtacaagca gctttggggg
    18781 atgaagtcca agtaccaact gtccatggag atgtgaaatt gaagatccct gctggaacac
    18841 agacaggtac gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa
    18901 caggcgacca acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa
    18961 gagaagcatt acgtgcattc gctgaagctg gcggcatcaa agttgaagaa cagcaagaag
    19021 acggtttctt cgataagttt aaagatgctt ttggcgggaa aaagcggaag tagtttttct
    19081 agtaaaaacg ctaagtcatt aataattaaa aaccagacga acaatcatat gttcgcctgg
    19141 tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca gctttagaat atgataatcg
    19201 tcatagtttc taggggtata accagtgaga gaatggaact tgttcaatcg atattgcaag
    19261 gtgtttttat gaataaataa ttcttctgag caatttttga tgcttccgtt atttttactg
    19321 aagcatgcca aagtcttctt gaacaaatgt acttcttctt ctgtcaggct tttcaaaata
    19381 cgttttacat ataatgttcg ttgatctgac gggattgctg gtaaaacgat cccgaggtct
    19441 aaatcttcaa aaaagcggat ctcgttcaca gattgatgga aagctatcca ttcgcaaact
    19501 tgtttggcat gaaggaaaga ttgcggtgtc tcttgctctg tataacaaag cgacccaatt
    19561 ccaaagttta gctgtaactg atacatttta ttaaactttg tctggatttt atcaagcaat
    19621 gatagcaatg aggctctgga aatccgattg actaggataa tgatttgttg tctatgtaca
    19681 gtgagcaaag cctgtccgta ataagagaag accctttcga gcaactccaa taaaggattg
    19741 atccgttctt gacacaattg ctgattgtca gaaagttgtc ccacaactac ataatatgga
    19801 gaagaaaaat caagctcaat taagtgaggg tggtatcgac tatgtacttg atatcatcaa
    19861 tgaaccgatg gtactttctt atgcgatgga acaggtagag cgattgcttg tttgtgcagg
    19921 aaaacaagcg atgcggctga tacgattagg tgagaaaata agaagataaa aatacacggt
    19981 cccttcatgg cgaccgtgca tttttggttt ttattaaata tttaatactt tattcaagaa
    20041 atcttttgtc cgttcatttt gcggattttc aaagatttgt tgaggcgttc catcttctaa
    20101 aaagtttccg tcgtcaatga acatgacgcg attggccact tctttagcga accccatttc
    20161 gtgcgtaacg ataaccatcg tcataccttg ttctgctaac tttttcatga cgttcaagac
    20221 atcaccgacc atttcaggat cgagtgcaga agtaggctca tcaaaaagca tgatgtcagg
    20281 gttcatcgct agagcacgag caatagccac acgctgtttt tgtccaccag aaagcatttc
    20341 aggatacata tcttttttat cttctagacc aactgtctgc agtaatttga ttgctttttt
    20401 ctctgcttct tctctagatt ctttcttcag gtccatcggt gctaaaatga tgttttccaa
    20461 aatagaaaga tgagggaata ggttaaagtg ttggaatacc attccgatat gttgtctcac
    20521 caagttgata tctgtgcttt tatccatcaa gtgggcgccg tcaataataa tttcaccgtc
    20581 agaaggttct tctaaacgat tcaagcaacg caagaatgta ctttttcctg aacctgaagg
    20641 gccaatgata cagacgacat ctccttcgtt gatggataca ttgatgtcgt ttaatactgt
    20701 gttatcgccg tattttttga ctaaatgctc aactaagatt ttttctgcca tcttacttca
    20761 ccttcttttc taaaactttt gctaattttg tcaatgctgt gatcaaaatc aagtacatga
    20821 tcgcaatgat caagtaaaca tacgtacttt gtgttgtacg tgcaacaatg atcttaccag
    20881 tttgcagcaa ttcgactaca ccgattgcgg aaatgatcgt tgtgtctttt aatgagataa
    20941 cgaactgatt gacgaatgat gggatcatga tgcgtatcgc ttgcggcaaa atgatttttt
    21001 gcattgtccg attataagaa agacctaaac ttctggaagc ttccatttgt ccgacaggaa
    21061 cggcgttgat cccgcctcga acaatctctg cgatataagc acttgcgtta agagtcaagg
    21121 tgataacacc agcaaggaag tcaggaatcg taaatccaac gataccaggc aacccaaaga
    21181 aaataaagaa cgcaagaacc atcattggaa ttcctcggat gatatcgaca taaatactag
    21241 caattgtgcg aagagcgcgt attggtgcta cactgaataa tccaaaaatc acaccgatta
    21301 ctatagccaa agcaaaggag atcaaagtta ggagaattgt tctccaaagt cctttcaaca
    21361 atgcttgata gttgttttgg ataagtccaa gaaatgttga ttcatcagca gtggaagact
    21421 cactggaagt ttctgaacca tccgctacat attctttaat aatcttatcg tattcgcctg
    21481 ttctttttaa ttctttcaat ccttcattga acatattaag aagctcagga ttttgtcctt
    21541 ttttaacggc aaatgcatat tttccgccag attctttttt gatcggtgtt tccaattctt
    21601 gtccttgttt gatcgcataa ccgattaccg gataatcatc catcattgca tcgattgcgc
    21661 caacttttaa cccgtcgtac aattgatcag cagcatcaaa atatttgata gaatagccat
    21721 attcagattc atggtcgctt aaaaattcag cactttcggt accaactttt gctccgactg
    21781 tttttccttt caaatcttca taagacttga tcgaagtatc tccttttttg acagctaatt
    21841 ggatcccgct atcaaagtaa gaatcagaaa aatcatagct tttctttcgt tcatctgtta
    21901 cagtcattcc ggcaatcatt ccgtctgctt gtcctgattc caaagcttgc aaagcagcag
    21961 agaaaccacg gtagtccatt cggatgttaa atccttgcag tttggcgatg ttttccatca
    22021 attttacatc gatgccttcg tactcgccct ttgcgttttg aaattcaaat ggagcaaaag
    22081 cagagtcact ggcaattacg tatacgtctt ttttgggctc tattttttgc attgtctcgc
    22141 ttgtgcttga ctctgccgtt gtagtgttgt cagcttgtgc aacatgacca aacgcaagtg
    22201 tcacaagtcc tgtcattaag aaaacaaatg agtgaaataa atgctttctt ttcataatca
    22261 acctcctaaa tttccaatat tttattatta taggcgaaaa gtgctgaatc agacaagact
    22321 gtaagctcga aaaaatgaaa aaaatgcgag tttctaacat tttatgttat aaaacataac
    22381 atgtataata agataaaaaa gtggcgaaag tatgttcggt gtggtataat tttttagtaa
    22441 ggaatgcctt tgctttcctt ttctttttat tggtcgtaaa atgaaaagga atgaatgaaa
    22501 aggaggacga agaatgattg agagagatac ctcccgacac tttgagcttg tctcgaaata
    22561 ccaaccggct ggtgatcagc ctgaagcgat taaccaatta gtggatggcg tagtcggcgg
    22621 gaaaaaagca caaatcttat taggtgcgac tggaacaggg aaaacatata cgatttccaa
    22681 tcttattgag aaagtgaata aaccaacact gattattgcc cataacaaaa cattggctgg
    22741 tcagctgtac ggggaattca aggaattttt ccctaacaat gctgttgaat attttgtttc
    22801 ttattatgat tattatcagc ctgaagctta tgtgccttca agcgatacgt atatcgaaaa
    22861 agattcaagt gtaaatgatg agatcgacaa attacgacat tcggctacta gttcattatt
    22921 agaaagaaat gatgtgatcg tcattgcatc cgtttcatgt atttttggat taggctcgcc
    22981 ttttgagtac caaaaacaag tagtttcgat tcgacaggga gcagaactag atcgcaatca
    23041 gttgattcgt gacttagtca gtatccaatt cgaaagaaat gatatagatt ttcagcgcgg
    23101 tcgttttcga gtgcgcggtg atgtcgtgga gatttttccg gcttcaagag atgaacgtgc
    23161 cttacgtgtg gagttttttg gagatgaggt cgaacgtatc cgagaagtca atgcactgac
    23221 aggtgaagtt ttaggagaaa cggaacacgt agcaatcttc ccggcgactc actttgtgac
    23281 gaatgatgaa catatggagc atgccgtagc gaatatcaaa gaggaattag aacagcgttt
    23341 aactgtcttg cgaaatgaaa acaaacttct ggaagctcaa cgtttagaac agcgaaccaa
    23401 ttacgatatt gaaatgatgt tagaaatggg ctacacgtcg ggaattgaaa attattctcg
    23461 gcatatggac ggacgtaaag aaggtgaacc gccttatacg ctattggatt tctttcctga
    23521 agattttttg atcgtggcgg acgaatcgca cgtaaccatg cctcaaatca gaggaatgta
    23581 taatggtgac cgagcgagaa aacagatgct agtagattac ggtttccgtt tgccatctgc
    23641 tttagacaat cggccacttc ggttagaaga gtttgaaaaa catgtcaatc agatcattta
    23701 tgtctctgcg acgccaggac catatgaaca tgaacaaaca gataccgtta tccaacaaat
    23761 catccgtccg acagggctgc ttgacccagt gatcgaagtt cgtccaatca tgggacaaat
    23821 cgatgatcta gtaggcgaaa taaatgaacg agtcgaaaaa gaccagcggg tgtttgtaac
    23881 gacattgacg aaaaaaatgg cagaagacct cacagattat ttcaaagaat tagggatcaa
    23941 agtcaaatat ttgcatagtg atatcaaaac gctggaacga acagaaatca ttcgagatct
    24001 tcgattaggt gaatttgatg tgttagttgg aatcaatttg cttcgtgaag gattagacgt
    24061 acctgaagtt tcccttgttg ctatcttgga tgcggataaa gaaggctttt tacgtagtga
    24121 acgttcctta gtccaaacga ttggacgagc tgctcggaat gaagaaggta aagtaatcat
    24181 gtacgcagac aaagtaacag attctatgcg tctagcgatg gatgaaacgt caagacgtcg
    24241 tacgattcag cagaagtaca atgaagaaca tgggatcgtt ccaaaaacga tcattaaaga
    24301 aatccgagat ttgatttcta ttacgaaaga atcggaagat gatacaaaag aagcggtaca
    24361 agtctcgtac gaagaaatga cgaaggaaga aaaagacacg ttattgatga aattagaaaa
    24421 agaaatgaaa gatgcggcaa aagctttgga ttttgaaaca gccgcgaatg tgcgagatat
    24481 gattttagaa ttaaaagctt caaagtagga ggacaacatg gctaacgata aaattacgat
    24541 tcatggtgca cgtgcccata acttgaaaaa tatagatgta acgattccac gagataaatt
    24601 cgtggtcgtt acaggtttgt ccggttctgg aaaaagttcc ctagcatttg atacgctgta
    24661 cgctgaaggg cagcggagat acgtagagag cctttctgct tatgctcgtc agtttttagg
    24721 acaaatggat aagccggacg tagatagcat cgacggattg agtccggcga tttctattga
    24781 tcagaaaaca acaagtaaaa atcctcggtc gacagtaggt actgtgacgg aaatcaatga
    24841 ctatttacgc ttgttgtttg cacgtgtagg acatccgatt tgtcccaacg atcatatcga
    24901 gattaccagt caatcagtag atcaaatggc agatcaagta atggctttgc ctgagcgcac
    24961 aaagatccaa gtgttggcac cgatcgtggt aaagaaaaaa ggacaacata aaaaaatatt
    25021 cgaacgaatc caaaaagagg gatacgtccg cgttcgagtc gatggtgaga tctatgattt
    25081 aagtgaagtg cctgagctgg agaaaaacaa aaaacatgat atcgcgatcg tcatcgaccg
    25141 gatcattgtg aaagaaggga ttcgttctcg cctatttgat tctttagagg cggccctgcg
    25201 attagcagac ggctatgctc tggttgacgt gatcggtgaa gaagaaatgt tgtttagcga
    25261 acactatgcc tgtccttatt gcggatttac tgttggcgaa ctagaaccaa gactgttctc
    25321 attcaatgcg ccttttggag cttgtccaga ctgtgatggt ttaggtgtca aattagaagt
    25381 cgatcaagat ttagtgattc cagatagaac gaagacactt agtcaaggtg ctatcgttcc
    25441 atggaatcca atcagctctc agtattatcc tcaaatgtta gcgcaggctt gtgaaagttt
    25501 cggtatcgat ttagatacac cattcgaaga attaccggaa gagcatcagg agatcatctt
    25561 gaatgggaca gatgatcttt tccatttcca ttatgaaaat gattttggtg gcgttcgcga
    25621 tgtagaaacg actttcgaag gggtattgaa gaatatcgag cgtcgttatc atgatacgaa
    25681 cagtgatttc acgcgtgagc aaatgcgtct ttatatgaca gaattaactt gtcagacatg
    25741 taaaggttat cgattgaatc cccaagctct atctgttcaa atcaatggaa cgaatatcgg
    25801 gcaaacaaat gagttgtcta ttcaaaaagc agtagatttc ttctcaaact taacgttatc
    25861 tgaacaagag aaagtaattg ccaaaccgat tttaaaagaa gtaaatgaca gactttcatt
    25921 cttggaaaat gtgggactag actatctaac tttgagtcgc gcttccggaa cattatctgg
    25981 gggagaagcg cagcgaatca gattggctac acaaattgga tcgaatcttt ctggagtttt
    26041 gtatatttta gatgaaccat cgatcggatt gcatcaacgc gacaacgatc gactgattgg
    26101 ctcattaaag aaaatgcgtg atcttggcaa caccctcgtt gtagtcgaac atgatgaaga
    26161 tacgatgtgg gcagcagatt atttgattga cgtcggacct ggggccggag aacaaggcgg
    26221 acaaatcgtg gctgcaggca ctcctgaaga agtagctaat gatgaaaatt ctttgacagg
    26281 gaaatatctt tcagggaaaa aatcaatccc tgtaccaaaa gaacgccgta aaggtaatgg
    26341 gaaagcaatc aagatcactg gtgcttcaga aaacaatctg aaaaatatta gtgttgaatt
    26401 tccattagga gaatttgtag cagtcacagg tgtgtctggt tctggaaaga gtacattggt
    26461 caatagtatc ttaaagaaat cgttagcgca aaaattaaat aagaattctg ctaagccagg
    26521 taaattcaag acaatttccg gctacgaaag tatcgaaaag atcatcgata tcgatcaaag
    26581 cccaatcggc cggacgccga gaagtaatcc agcgacttat acaagtgtat ttgatgatat
    26641 ccgtgggtta tttgctcaaa cgaacgaggc aaaaatgcgg ggttataaga aagggcgttt
    26701 tagtttcaac gtaaaaggcg gtcgttgtga agcttgtcgc ggggatggaa ttattaagat
    26761 cgaaatgcac tttttgcctg atgtctatgt tccttgtgaa gtatgtcatg gtaaacgata
    26821 taattctgaa acattagaag tgcattacaa aggaaaaagc attgctgata ttttggaaat
    26881 gacagtagag gatgctgtag aattcttcaa gcacattcca aagattcatc gcaaactgca
    26941 aacgattgtt gatgttggct taggttatgt gacgatgggg caaccagcaa cgacattgtc
    27001 cggtggtgag gcacaacgga tgaaacttgc cagtgaattg cacaaaatct ctaatggaaa
    27061 gaatttctat atactagatg aaccaacgac aggacttcat agcgatgaca tcgcccgctt
    27121 gttgcatgta ttacaaagat tagtagatgc tggtaacaca gttttagtga ttgaacacaa
    27181 tctagatgta atcaaaacag cagattatat cattgattta ggaccagaag gtggagaagg
    27241 tggaggaacg atccttgcga ctggaacacc agaagaaatc attaacgtaa aagaaagtta
    27301 tacaggtcac tatttgaaaa aaataatggt ataaagagga tagatagctg agatagttaa
    27361 aaaaacataa aaaaatgaat ttttgcttgt tttattaata tcagtgttct agaatcatta
    27421 catggtagtt ctttctacac tgattggtat agataaaaac ttcctataag ttcaatccgt
    27481 tcaaaaaaga attgagcaat cggaggactt tcctttgatt gcttttttct ttatggtatg
    27541 atattccttg tgtagttgga aaagaaaaac aagagattat gagtaagtga gagataatga
    27601 tgaacagtat tgaaaatgaa gaatttatcc ctatattatt aggaagcgac atgaacgttt
    27661 atggtatggc acgttctttt aatgaagcat acggcaaaat ctgccaagca tatgcttcgg
    27721 atcagttagc ccctacccgc tacagtaaaa ttgtcaatgt agaagttatt ccaggatttg
    27781 ataaagaccc tgtatttatc gagacgatgc ttcgtttggc aaaagaaaga tattctgata
    27841 agagtaaaaa atatcttttg attgcttgtg gtgatggata tgcagaattg atctcccaac
    27901 ataaacaaga gctgtcagaa tattttatct gtccatatat tgattattct ttatttgaaa
    27961 ggctgatcaa taaagtaagc ttttatgaag tttgtgaaga atatgatctt ccgtatccta
    28021 aaacattgat cgtcagagaa gaaatgttag taaatggaca tttggaacag gaattgcctt
    28081 ttgaattccc agttgctttg aaacctgcaa acagtgtgga gtatctttct gtccaatttg
    28141 aaggaagaaa aaaagctttt attttagaga ctagagaaga gttcgattta attttaggcc
    28201 ggatatacga agccggttat aaaagtgaaa tgattgtaca agattttatc ccgggtgatg
    28261 atagtaatat gcgtgtgcta aatgcgtatg tagatgaaga tcatcaagtt cggatgatgt
    28321 gccttggtca tccactatta gaagatccta caccagcatc gattggtaat tacgttgtga
    28381 tcatgccaga ttataacgaa aaaatctatc aaaccattaa ggcatttttg gaaaaaatcg
    28441 aatatacagg ctttgccaat ttcgatatga agtatgatcc tagagatgga gaatataaat
    28501 tatttgaaat caatttgaga caaggtcgta gcagtttctt tgtgacattg aacggcttga
    28561 atctggctcg ttttgtcaca gaagatcgag tgttcaacaa accctttgtt gaaacgacct
    28621 atggaacgaa tcaatcagat aaagcaagat tatggatggg tgtgcccaaa aaaatctttt
    28681 tagaatatgc tcgggaaaat gaagacaaaa aactagctga gcaaatgatc aaagaaaatc
    28741 gttacggtac tactgtattt tatgaaaaag accgatcaat caagcgctgg ttgttgatga
    28801 aatatatgtt ccataactat attccaagat tcaaaaagta ttttcatgtg aaagaaggct
    28861 agttcatgga gaattttttc agtattttag gcggaatggg cacgatggcg acagaaagtt
    28921 ttgttcgctt gatcaaccac cgaacgaaag ctacaaaaga tcaagaatat ttgaattatg
    28981 tcttattcaa tcatgcaaca gttcctgatc gtacagctta tattttagat cgatcagaag
    29041 aaaatccaat gccgtttctg ctggatgata ttgagaaaca aaatttattg cgaccaaact
    29101 tcattgtgtt gacttgtaac accgcacact acttttttga agagttacaa gcggcaacgg
    29161 atattcctat cttgcatatg ccaagagaag cagcaaatga acttgtccgt cagcatacga
    29221 cagggagagt agcgattcta ggaacagaag gaagtatgaa agctggaatc tatgaacgcg
    29281 aagtcaaaaa tcttggtttc gaaacagtga ttcctgatac tgcgcttcaa gaaaaaatca
    29341 attacctgat ctatcatgag atcaaggaat cggattattt gaatcaagag ctctattacg
    29401 aaatcttaga ggaagcggta gagcgcctaa actgtgaaaa agtcatccta ggttgcacag
    29461 aattatcttt gatgcatgaa tttgctgaag ataatcatta cccagtgatc gatgctcaat
    29521 ctattttagc ggatcgaacg atcgaacgtg cattagctga aagaagcgag gcattggata
    29581 cagcatcgga aaagtaaaaa aattggattt ggggcaaata tcctaaaaga tatttggtcc
    29641 aaatcttttt gtaaaagaaa aagaaaactg gtaatgatac gcccgggatg atataatttt
    29701 attatgcaca atgtgagagg ggttcgtaaa tatgacagat aatttgcaat tagtcatcat
    29761 aacaggtatg agtggtgcag ggaaaacagt agctatccaa agttttgaag atatgggcta
    29821 tttttgtatc gataatatgc cgcctcgatt gattccaaaa ttctgggaat tgatcaaaga
    29881 atcaggtaaa gtcacaaaaa tggctttagg agtggatttg cgttctcgtt ccttctttga
    29941 agaaatccaa aacatgctga tcgaaatcga aaatacgaac ttcatcgaca cttctatctt
    30001 atttttagat gcttcagacg aagaactagt ggctcgctac aaagaaacaa gacgagcaca
    30061 tccaatggcg atggacggtt tgatcactga agggatacgt aaagaacggg cgatactaga
    30121 cgacttgaaa gcccaagctt ccgtgatcat tgatacgaca acattgacac cacgacaatt
    30181 acgtgaaaaa atcaatgagt cattcaaatc aaagggagac caaggatttc ggatcgaatt
    30241 tgtatcattt ggttttaaat acgggttgcc gatcgattcg gatattgtga tggatgtgcg
    30301 ttttttgccc aaccctcatt atatcccgga actaagacca ttgaccggaa aagatcagcc
    30361 agtctatgat tatgtgatgt cctttgatga aacgcaatca ttctaccata aatttttgga
    30421 tcttctggaa actgtattgc ctggatacat taaagaagga aaaagcagct tgacagttgc
    30481 aatcggctgt acaggcggtc aacaccgttc tgtggcattg actgaacgtt tggcaaatgc
    30541 tttagggaaa aactataaag tcaatgtgac acatcgggac aaggataaaa ggaaagagac
    30601 ggtgaaccgg tcgtgaaaat gaaaacctac cggataagaa aaccaaagat cgtagtggtc
    30661 ggcggtggca ctggtctgcc agttatcctg aaaagtttac gaaatcaagg agcagatatc
    30721 acagcagtag tgactgttgc agatgatggt ggaagcagcg gtgcaatcag agaatcgatc
    30781 gctatggctc ctcctggtga cttgagaaat gtcttagtgg cgttatcgga tatgccacaa
    30841 ttctatgaag atattttcca ataccgtttc aaaaaggaag atcagttttt agccaatcat
    30901 acgatcggta atctgatcat tgccgcagtt tccgagatgc ggggaagcac ttatgaggcg
    30961 attcaattat tagcaaaaat gatgcatgta gatggacatg tttatccttc ttcagaaact
    31021 cctttgacct tgcatgctgt ttttaaagac ggaacgactg cagtaggaga atctaaaatc
    31081 gcgatcaacc gtaaaaccat tgaccgtgtg tatgtctgca acacaaatga taaaagtgaa
    31141 gccaaagcgg ctcgaaaagt cgtttcagca attatggacg ccgacatggt cgttttggga
    31201 cccggaagtt tattcaccag tatccttcca aatttagtga taccagaaat cggcgaagca
    31261 atgctcaaga caactgctga aaccgtctat atctgtaata tcatgaccca aaaaggggaa
    31321 acggaacatt tcacagatgc cgatcatata cgtgttttac acaagcattt gaatcagcct
    31381 tttatcgata cagtattggt caatacggaa aaagtgcctg aagattatat ggacccagag
    31441 atctatgatg agtatctagt acaagtcgca cacgacttca acggattgag aaatgaagga
    31501 tgtcgagtga tttccaccga ctttttagaa ttgaaagatg gtggtgtctt ccacgatgga
    31561 gacaaagtag tagaagaatt gttccgttta gttttcggtt caaaatatta agcaatttat
    31621 tgttggagaa aggaggagaa taggatgtca tttgctgcag atgtgaagaa agaactaact
    31681 ggtttggaag ttcatcgaga acatgcgaaa gcggaattag ctgctttgct gcgaatgaac
    31741 ggctctttag gtattgtgaa ccagcagttt attttgaacg tccaaacaga aaatgctgca
    31801 attgccagac gtatctattc tcttttgaaa gatcattatc aggtacgaag tgagctactc
    31861 gttcgtaaaa aaatgaaatt aaaaaagaac aatgtctata tcgtccggtt gaagcaaggc
    31921 acaaaagatg tgttgatgga tcttgatatc atggatggta tgatgttcaa tggacacgtt
    31981 tcggacgaga ttatgggaaa ttcgcaaaaa atgcgttctt acttacgtgg tgcttttatg
    32041 gcctcaggtt cagtcaataa tccggaaacg agtcgttatc atttggaaat attttctatt
    32101 tatgaacagc ataaccaaga catttgcgat atgttaaatt attacggtct acatgctcga
    32161 acgcttgaac ggcgcaatgg ctatatttcg tatttaaaag gagcagaaaa aattgctgat
    32221 ttcttaacat tgattggtgc aaccaactca atgctccgat ttgaagatgt tcgaatcgta
    32281 cgggatatga gaaattcggt caatcgtcta gtcaattgtg agacagcaaa tctgaacaag
    32341 acgattgatg cggcttctaa acaaatcgaa aacatcgaat tgatcgaagc aaaggtcggt
    32401 ctacatgccc ttcctgaaaa acttcaggaa atcgcagaac ttcggcttca acatccagag
    32461 gtcagcttga aagaattagg ggaaatgatt ccctcaggtg caatatcaaa atcgggaatt
    32521 aatcaccgaa ttcgcaaaat aaatgaattt gcggaaaaac tgcgtgaaga agcagtttaa
    32581 tccaaagaaa aaactgatac atgaagcagg agatgcgaat gtatcagttt tttttaaaag
    32641 ctatacttgc ttttcttccg tcaaactata attccaactt tactggagta acataatgtt
    32701 gaaaggcttc tattgttttt taatacaatc gtataaaaag cggggagaca actgaatgaa
    32761 gattttagtt ttatacataa acttttgctt aatatagaag tcctgatata gataggatca
    32821 agtatttatc tataactgga gattaattaa aaactggaag gaactgctcg atcaaccttg
    32881 agaactgttt ttcttacttc tggtcaagat tctcgtggtt ggtggataag gatggttgat
    32941 aagttcattg gattagctag tcgaaaacaa ccaggtgtaa ttaatgtaga agttttaaca
    33001 aggggatgga gtgatttatt cagtgataat cataagttca taaatagtaa ttgggatgta
    33061 tggttggtat acgttatacc tgaacttgaa ctatcaagat ttggtttaga atttgagaat
    33121 cgatgtttat aaagggaggg caaaaaatgt gcttttttac aacttttact atcattatga
    33181 ttgcagtact ttttattttt ctagatatag caaagcgaaa tacagcattt ttattatatc
    33241 gggttttact tcgagcaggt ctcataacat ttatatcgat cgttggattt tttctattca
    33301 cagttattgt atttatttgg agaactacag caccaccatt gccagagatt acatatggag
    33361 aatttccgtt tagtcttgaa tatgaaatag atgaggaaag aattttgatt gaagatacgg
    33421 tgattgcaga atttatagga agttttagag ggaatgtgac aactcaacct agacgaagtt
    33481 ggagtataaa acttttaaat aatagtggag agttgaaagc atttcattat gaattcttaa
    33541 taatagaaag tgaagatgta acaatcactt taagtagtgg ctctgctgga tattttatga
    33601 atgatggtgg aagcaagcca cagctcacag tccgtgatcg tattgcaggt tttatgcatt
    33661 tttctcacta tgacttagaa gaagctcgta aaatacttgc agaatatggg atcacattaa
    33721 tcaattggga atatgcgcca ccgataagaa attttttcaa tgattattat tagtcttata
    33781 aaaaacagaa tgtaaaattt atgtgcgcga gtatttagtt ttccattttt tgtttgatag
    33841 atatatagca ttgttgaagc atgggatagt ggtgccaatt ttatcaatga ggttggtcag
    33901 gcagttaaca taattggtga tgctataaat ccatttaatt ggaattggag gaattaggtg
    33961 tggaaacagt gagattatgg atattcatga aaaattttcg ttcggctttg agatatgtta
    34021 ttctggaatc aaaatcaggt tattatctga ttgacacaga tcactatact ttttggggag
    34081 gctacctttt tccgattttt aattggataa tcccccataa ggttatgaaa attaacttgc
    34141 ctgcagaaga aataaataga ttaatactca acaaagattt acaaaggaaa agagagaaag
    34201 aacattctaa tgttgcttgg ataggtccag catttgcagt agtgacttct tcaattgtcg
    34261 gtcctatgct agagagtatt tatagtgatt tttctattgg aatacgttat ttatttgtta
    34321 ctttaagtat agctgttctt ttgtgcataa agggttatgt tagcaagaag aataaagata
    34381 ttttaacact aatcggtgta aacaatttat ctagtactaa aattatactg tttcctgaat
    34441 ctataaaaga aggatcaagg ttgttgataa tttttataat gcttatactg ttttctgttt
    34501 tatttggggt gatgtttata ga
//
LOCUS       DACEPD010000019        32460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.019, whole genome shotgun sequence.
ACCESSION   DACEPD010000019 DACEPD010000000
VERSION     DACEPD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.019"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..328)
                     /locus_tag="IW797_000929"
     CDS             complement(<1..328)
                     /locus_tag="IW797_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ1223719.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNI"
     gene            complement(338..1768)
                     /gene="gatB"
                     /locus_tag="IW797_000930"
     CDS             complement(338..1768)
                     /gene="gatB"
                     /locus_tag="IW797_000930"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ1223720.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(1765..3237)
                     /gene="gatA"
                     /locus_tag="IW797_000931"
     CDS             complement(1765..3237)
                     /gene="gatA"
                     /locus_tag="IW797_000931"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ1223721.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(3237..3542)
                     /gene="gatC"
                     /locus_tag="IW797_000932"
     CDS             complement(3237..3542)
                     /gene="gatC"
                     /locus_tag="IW797_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ1223722.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(3648..5684)
                     /gene="ligA"
                     /locus_tag="IW797_000933"
     CDS             complement(3648..5684)
                     /gene="ligA"
                     /locus_tag="IW797_000933"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ1223723.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(5701..7935)
                     /gene="pcrA"
                     /locus_tag="IW797_000934"
     CDS             complement(5701..7935)
                     /gene="pcrA"
                     /locus_tag="IW797_000934"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ1223724.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            8090..9649
                     /locus_tag="IW797_000935"
     CDS             8090..9649
                     /locus_tag="IW797_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ1223725.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(9760..11691)
                     /locus_tag="IW797_000936"
     CDS             complement(9760..11691)
                     /locus_tag="IW797_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1223726.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(11709..12623)
                     /gene="pfkB"
                     /locus_tag="IW797_000937"
     CDS             complement(11709..12623)
                     /gene="pfkB"
                     /locus_tag="IW797_000937"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1223727.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(12620..13372)
                     /locus_tag="IW797_000938"
     CDS             complement(12620..13372)
                     /locus_tag="IW797_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1223728.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(13531..13740)
                     /locus_tag="IW797_000939"
     CDS             complement(13531..13740)
                     /locus_tag="IW797_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223729.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(13751..15079)
                     /locus_tag="IW797_000940"
     CDS             complement(13751..15079)
                     /locus_tag="IW797_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223730.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(15444..16031)
                     /locus_tag="IW797_000941"
     CDS             complement(15444..16031)
                     /locus_tag="IW797_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ1223731.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(16114..17364)
                     /gene="clpX"
                     /locus_tag="IW797_000942"
     CDS             complement(16114..17364)
                     /gene="clpX"
                     /locus_tag="IW797_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ1223732.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(17611..18900)
                     /locus_tag="IW797_000943"
     CDS             complement(17611..18900)
                     /locus_tag="IW797_000943"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ1223733.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(19054..19986)
                     /locus_tag="IW797_000944"
     CDS             complement(19054..19986)
                     /locus_tag="IW797_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223734.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(20143..20802)
                     /locus_tag="IW797_000945"
     CDS             complement(20143..20802)
                     /locus_tag="IW797_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ1223735.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQMIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(20805..21830)
                     /gene="sppA"
                     /locus_tag="IW797_000946"
     CDS             complement(20805..21830)
                     /gene="sppA"
                     /locus_tag="IW797_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ1223736.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(21843..22133)
                     /locus_tag="IW797_000947"
     CDS             complement(21843..22133)
                     /locus_tag="IW797_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223737.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            22542..24347
                     /gene="glmS"
                     /locus_tag="IW797_000948"
     CDS             22542..24347
                     /gene="glmS"
                     /locus_tag="IW797_000948"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ1223738.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(24419..25774)
                     /locus_tag="IW797_000949"
     CDS             complement(24419..25774)
                     /locus_tag="IW797_000949"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ1223739.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(25798..26967)
                     /locus_tag="IW797_000950"
     CDS             complement(25798..26967)
                     /locus_tag="IW797_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223740.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(26964..27851)
                     /locus_tag="IW797_000951"
     CDS             complement(26964..27851)
                     /locus_tag="IW797_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ1223741.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(28040..31732)
                     /gene="nifJ"
                     /locus_tag="IW797_000952"
     CDS             complement(28040..31732)
                     /gene="nifJ"
                     /locus_tag="IW797_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ1223742.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            31931..32452
                     /locus_tag="IW797_000953"
     CDS             31931..32452
                     /locus_tag="IW797_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ1223743.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         8783 a         7063 c         5872 g        10742 t
ORIGIN      
        1 taatattatc tctaggaatt tttaatgctc gagcatagtc gttcgttgta ccagctggga
       61 tgatggccat tttaggacgg tgttcaagcg gagcgattcc attaaccact tcgttgattg
      121 tgccatcgcc accagctgcc actactagat caaaacctag ttctgcgact ctacatgctt
      181 cattcttggc tgaatctggt tctggtgtag ttgcataggc gctggcttca aatcctgctt
      241 cttctaatgc ctgtaggata tccgccaaat tttttttcaa taattctttt cccgaggtgg
      301 ggttataaat tacgcgtgct tttttcattt tcagcctcta tcgtttcgct aattcatctt
      361 taagtagctt attcacgaca ccaggatttg cttgtccttt agttgctttc atgatttgac
      421 cgactaggaa gccaaccgca cgatcttttc catttttgaa atcatcgaca gattgttggt
      481 tgttatccaa aatttcatta atgattggca gtagtttgtt cggatcagat aattgcaccc
      541 aaccattttt ttcaactact tcttgggctt ctccgccatt tgtgatcaat tcacggaaga
      601 cttttttggc gatttttgag ctgatcgttc catcggcgat caaacgaatc attcctgcta
      661 agttctcagg tgtcaatttt gtctcagcta attccagttt ctcactgttc aagtaagcag
      721 aaacttcacc catcaaccag ttagatgctt gcttggcatc tgcaccattt tccaaaacag
      781 cttcaaaaaa atcagacatt tcttttgtaa gtgtcaatac cattgcgtca tactctggaa
      841 gtccaaactc acggatatag cgttcgcgac gagaagctgg catctcaggc aaactagctt
      901 ttactttttg aatccattcg tcatcgatca cgaatttcgg gatatcaggc tctgggaagt
      961 aacgataatc actagaccct tctttgacac gcatcaaaac tgttttattc gttgtttcgt
     1021 caaaacggcg tgtttcttgt tggatttctc ctcctgaaag aagaactttt gcttgacgtt
     1081 tttcttcaaa agctaaacct tttttcacga aagataaaga gttcaagttt ttcaattctg
     1141 ctttcgtacc gaactcttct tgtccataag ggcgaagaga aatattggca tcacagcgca
     1201 tagagccttc ttccattttc acatcgctga cttctgtaaa ttggataatt gatcgcaatg
     1261 cttctagata agcgtatgct tcttctggtg aacgcatatc agcttcagaa acgatttcga
     1321 tcaaaggcgt gccttgtcgg ttcaaatcaa cgtaagaata accgccaata ccatgcatgt
     1381 tttttccagc atcttcttct aaatggacac gttcgatccg gatttttttc tttttacctt
     1441 ctacttcgat ttcgatccat ccgtcatgac cgattggttg atcaaattgg gaaatttgat
     1501 atgcttttgg attatctggg taaaagtaat ttttccggtc aaaatgagta tctttagaga
     1561 tttcacagtt taatgccaaa gctgctttca tcccgaattc cagcgcagct ttattcatta
     1621 ctggcagcac gcctggataa ccccagtcga tcacgtttgt attgctgttt ggttctgcac
     1681 caaaatgagc tggcgctgga gagaaaattt ttgagttggt ttttaactcg acgtggactt
     1741 ctagtccaat gactgtttca aaattcattc gtctttccct cctaaaatag ctggttgttg
     1801 tttatggaat tctgttgctt gttcaaacgc atatgccgct tgatacatcg ttttttcatc
     1861 aaaataatta ccgatgatct gcaatccaac tggtaatcct tctgataaac ctgccggtac
     1921 agacatacct ggaagtccag ccaagttgac agggatcgtt aagatgtcac tcatgtacat
     1981 tgtgattgga tcgttaatgt tctcaccgat tccgaatgca actgttggtg aagatggccc
     2041 gatgatcaag tcataatctg caaagacttt ttcaaaatct cgtttgatca atgtacggac
     2101 ttgtcctgct tttttgaagt aagcgtcata ataacccgcg cttaaggaga atgtacctaa
     2161 catgatacgg cgttttactt cttcaccgaa tccttctgaa cgtgagttga catatacatc
     2221 atctaatgat ttcacatttt cagagcgata accgtagcgg ataccatcga atcgttgcaa
     2281 attggaactt gcttctgatg aggcaatgat ataataaaca gccacaccgt attttgaatg
     2341 aggcaagctg acttcttcca caacagcacc taatgaacgg aatgtttctg ctgctttcaa
     2401 tacagcttct tttacgccag gggccacacc ttcgcctaag tattctttag gtaaagcaat
     2461 cttcattcct ttgatgtcgc ctgtcagtcc ctctgcaaaa tcaggaactg aaacgcctga
     2521 agaagtaccg tctttttcgt cataaccgct gatagctgtc aatgctaatg cattatcttt
     2581 caccgtacgg gtcattggtc cgatttggtc aagagatgaa gcaaaggcaa tcaaaccaaa
     2641 acgtgagaca cggccataag taggtttcat ccctacgatg ccgttaaaag ctgctggttg
     2701 gcggatactt cccccagtat cactacctaa agaaactgga atttgtccag ctgcaactgc
     2761 tgctgcagaa ccaccagaag agccaccagg tacttttgtt gggtcccaag cattttttgt
     2821 tttcttgaaa taagaagttt ctgtacttcc acccatagca aattcatcca tgtttagctt
     2881 accaacaggg atcatatctg cttgatagat tttatccatg accgttgcat cataaattgg
     2941 ttcaaaattg taaaggatct tagatgcagc ggttgtcaag atatcttttg ttacgatatt
     3001 atccttgata ccaattggaa taccagcaag gacgttgctt tcatcaatcc ctttttcatc
     3061 taaagctttt gcctgttcca atgctttttc ttcgttcaat gtaatgaatg cctcgacctc
     3121 aggctctgtt tctttgattc gttcaaatgt tgtttttgtc aaatcttgta cagtgatttc
     3181 tttcgataca agcatatgat gcagttcttc tagagaacga ttgtgtaatt ctgtcattat
     3241 gcgccagcct ccccattatc gatgattgcg ggtactttga taaagccgtc ttctgtttcc
     3301 ggcacatttt tcaataattc atcgcggttc catccagctt gcgcccgatc ttgtcgcatc
     3361 acgtttactg tttcaaccac attcgacgta aatggaacac cttccgtatc tacttcttcc
     3421 agcatttcaa ccatatcgat gattttacct aattggtcgg taaaacttgc aagttcttca
     3481 tctgcgaacg caagttttgc taatttggca acgtgtttca cttgttcttc actaatcgcc
     3541 atactacccc ttctttctaa acgagttaaa aactttgctc tctattttaa cacaagagtg
     3601 ccgtgtctga aattgtttat tgaaatttgt ctgaaaaaac tcatttttca ttcttctact
     3661 gagcgacttt tagccaatgc atctgccatt tgatcttctg tccaaacttc gattcctaat
     3721 tcttgtgctt tggtcagctt gctgccagca tcttctcctg ctaccacgat atccgttttt
     3781 ttcgatacgc taccagtcac tttcccgcca agattttcga tcgtctcttt tgcttcttca
     3841 cgagtaaatc gtgttagttt accagtaagg actactgttt tttctttaaa aggagactcg
     3901 acttcttgca attgggcatt tcgaagccct ttgtattcga aattgacgcc tgcttgtttt
     3961 aattcattca tcaattcatg gacttcttcg ttactgaaat aagtgactac actgtcggca
     4021 atgatatcac cgatcgtatc aagcgcaata atcgactcat agtcactttt actcaatgtt
     4081 tctagatcac cgaaatgttc tgctaaaatt tttgctgctt tcgctccgac atgtcggatt
     4141 cctaacccga aaatcaaccg ctcgaccgaa ttatctttgc tgttgtcgat tgctgtcaaa
     4201 atattatttg ctgatttttc ttttattttg tccaacgtca ataactcttc ttctgtcaat
     4261 ttgtaaagat cagcaacatc tgctactagt tttttatcat acatctgttc tagtacgcgc
     4321 ggccccaacc catcgatatt catcgcattt cttgaaacaa agtggttcaa accttctttc
     4381 atttgagcag ggcatttagg attgatgcac cgtaaggcta cttcttcatc caaatggaca
     4441 agttcacttc cacaaactgg acaatgtgta ggcaattggt attcttcgct gtctttaggt
     4501 cgtttatcta acacgacttg cgaaacttct gggatgatgt ctcccgcttt ataaatcaga
     4561 actgtatctt tcaagcggat atccttcaac ttaatataat caccgttatg caggctggca
     4621 cggcttactg tcgttcctgc tactcggact ggtgtcatga tagctgttgg cgttactacc
     4681 cctgttcgac cgactgtcca ttcgatgttt tcgattagcg tttcaacttc ttctggcgga
     4741 aacttgtaag ctgctgccca tctcggtgct ttgatagtaa aaccaagttg atcttgtagc
     4801 gagaattcat tcactttgat gacgattcca tcgatttcat agggcaaatc cacacgtttg
     4861 tcatgatatt cttcaatgta tgaccacact tcatcaatcg aatgacatag acgtttttcc
     4921 cgattcgtgt ggaatccaat tttttctagc tcttctagcg catcatactg ggtttttgct
     4981 tgcataggac cgaaatcagc tactgtgtat aaaaaagtgt ccagatttct tttcgctgtg
     5041 attttagaat ctaactgacg caaagaacca gctgcggcgt tccgaggatt cgcaaagata
     5101 tcttttccct ctgcttctct gtcttggttc aattggacaa atgaccgttt tggcatgaaa
     5161 cattcgcctc ttacttcgat gttcattggt tcttttaatt tgattgggac cgaccggaca
     5221 gtttttaggt tttcagtgat gttttctcct acagttccat ccccgcgagt cgcacctctc
     5281 acgaaatttc catcttcata acgcaaagaa acggaaagtc catctatttt tagttcacag
     5341 caatagtcaa ctacacgtcc cactgctttt tgtacacgtt gatcaaaagc tatcagctct
     5401 tctttgctga aaacgtcgtt caaactgtaa agcggaatat catgagtgac tttttcaaaa
     5461 ccttctaata cttttccgcc tactcgttga gtgggagaat cagacgtgat caaatcagga
     5521 tattgttcct cgattgctac taattctgcg tattctttat cataaacata gtcttccact
     5581 gttggtttat cttctacgta gtattcgcga gaccattggt tcagtcttgt acgaagttct
     5641 tctgctcgtt tagcagcttc gtcaatcgtc atttcatgtt ccatcattta ccctccacta
     5701 ctaagctttt tcaattggcg caaaggcagc caaaagacgt tttactcctt gacttgggaa
     5761 agccacatct agttctagat ccttcgacgt cccgcttact cggacgactg ttcctagacc
     5821 ccattttttg tgtttgactt tatctccagg tttccagctg ttattttccc ctccagtagc
     5881 acttttagag ggaacaaccg ctgtctctgg ctgtttatag ctatatgctg tttttttagc
     5941 gaatacaggc gtttttttag gcgtaggacg catgccttcg atttccagta actcctgatc
     6001 gatttcttct acaaaacgag aaggtcggtt atattgcgtc cgtccatata atgttctgga
     6061 gaaagcattt gttaaataca acgcttcttc tgctcgcgtg atccccacat atgcgagacg
     6121 gcgttcttcc tccagttcgc tttcttccat caatgctcga gataaaggaa aaaccccctc
     6181 ttctagacca atcaggaata caactggaaa ttctaatcct ttggctgcat gcaaagtcat
     6241 caatgtcact tgggaagcat cttcttctaa attgtcaatg tcggaaacaa gtgccagatc
     6301 atttaagaag accgttagtt tttcttctgg tgcatcagcg tcttcttcat tttgctgttc
     6361 gaattgtttg tcgaattctt gtgtgaccgt taaaaattcc tccaagtttt cgatgcgtgc
     6421 ttgtgcttct aatgtatttt ggatttttaa gtcttctaga tatccgctac gatccaaaac
     6481 ctctttcgtc aattctgtca ctgtcaagta tgggatcatc tgagtgactt cttggatcat
     6541 ctctccaaat gcacctaatt gctgtcctgc ttttccaccg atatttgcca gatccacatt
     6601 ttgcgctgct tcaagcagtg gccattcatg aagagaagca aaactgcgta gtttttcgat
     6661 agatcctggt ccgatgcctc gtttgggcga attaacgata cgctcaaaac tgattgaatc
     6721 ttgaggattt accagcacat tcaaatacgc taaaatatct ttgatttctt tacgatcgta
     6781 gaacttgtgc cctccaacca ttttataagg aatatttgct ttcaacagag tttcttccat
     6841 tacacgagat tgtgcgttcg tccggtacaa gatggcaaag tcgccgtaat tccgatgatt
     6901 gcttcgaatc tcttcttgca tccggtctac gataaaacgg gtttcgtctc gttcattatc
     6961 tgcacggtag taagtgatct tgtttccttc tttgttttct gtccacaagt tttttggttt
     7021 tcgattgcta ttgttttcaa tgacttggtt tgctgcgcta aggatgtttt tcgtggagcg
     7081 atagttttgt tctagcaaaa ttactgcagc atctggataa tctttttcaa aatccaagat
     7141 gttctgcata tctgcaccgc gccagccgta aatactttga tctgcatctc ccacgacgca
     7201 caggttccta aatctacctg ccaataaatt gactagcgta tactgtgcgt gatttgtatc
     7261 ttggtactca tctacatgaa tataatggaa tttgttttga taatacgtga gtgaatcagg
     7321 atgttcttca aataatcgaa tcgtattcat gatcaggtcg tcaaagtcca tacattgatt
     7381 atattgaagc tctttttggt aagcagcata acattttgcc gcaatctctt cgtaataact
     7441 gccttgcatt tttgcaaaat cctgaggggt ctgcaggctg ttttttgctt gagagattgt
     7501 tcctaatata cttcgaggat catatttttt cggatcaatg ttcagttctt ttagtatccg
     7561 tttcatcaag gtcagctgtt cagaagaatc gatgattgta aagttccggt tatacccgat
     7621 aaaatcaaca tctcttctta aaatacgcac acacatcgag tgaaaagtcg atacccagac
     7681 atcttcgcct ccagatgcga gaatcgcatt gacacgttct ttcatttctt ttgcagcctt
     7741 attggtaaaa gtaatcgcta ggatgttcca aggattgact tctttttcct caatcaaata
     7801 ggcaatgcgg tgtgtcagca ctctcgtctt tccacttcct gcacctgcca ttagcagtaa
     7861 tggtccgtca gtgtgaagaa cagcttcttt ctgacggggg ttcatcccgt ttaataattc
     7921 agctttggat gacattaaca acatcctttc ttttatcaat cttgttaatt atagcatttt
     7981 cttggctttc attaaactag ctagttcaaa atcatttgtt tctttttatg gatttttcct
     8041 tgtcaattcg ttgatttcgc gctaaattaa aagaaagcga ggcgtttgaa tggaaaaaaa
     8101 aggaaaaatc aagaaattag ataagactca gatatatttt attctaaaag gaatatttgt
     8161 tggactgctg acaggaatcg tcgttagcct gtttcgatta tctatcgaaa agctaagcga
     8221 tttagttcga agtatctatg aaatgagtcg ggaacagccg atttacttaa ttggcctagc
     8281 tatttgctgc atccttgcag catttttcgt gggttatctg gtcaaaaatg aaccggatat
     8341 caagggaagc gggattccac aagtagtggg acagcttcgc ggggaattat caatgaactg
     8401 gttctctgtt ttatggaaaa agttcattgg cggtgtactt tcagttggtg cgggcctttt
     8461 tctaggtaga gaaggacctt ctatacagct tggcgcatcc attggacaag gagctggaca
     8521 attgtttcgg agtccatctt cagaagaaaa gattttaatt tctagcggtg caagtagcgg
     8581 acttgcagca gcattcaatg ctcctatcgc aggattactc tttgttttag aagaaatcca
     8641 tcactccttt tctccacttg tttggctgac atcatttgct tcagcaatca ctgctaactt
     8701 cgtctctctt tactttttcg gcttgagacc tgttttatat ctaggtgacc ttccttcttt
     8761 gccgttgaaa tattatggtt ccttagtgat cttaggagta cttctaggca tcttaggttt
     8821 tatttatcag aatgtcttgc tggctttgcc aaaattttac aaccgattgc ctttaccagc
     8881 ttattttcac gggttcgttc ctttcttgct gattttgcca attggttatt tatggcctag
     8941 tactctaggc ggaggcaatc aaatcgtttt agcttttgga catgcttcct tgccaatcat
     9001 tatgattatc ggtttgtttg ttcttcgatt cgttttttcc atgatttctt acggtggggg
     9061 tcttccaggg gggattttcc ttccgatatt gacattgggg gctttaatcg gtagctttta
     9121 tggaaacatt atggttgatt tcgggatgga tccaatctac atcaaggatt ttgtcgttat
     9181 tgccatggct ggttatttca cagcgattgg aaaagcaccg ctgacagcaa ttattttagt
     9241 gacagaaatg gttggctctc ttaatcatct gatgccttta ggattggctg ctcttgttgc
     9301 ttatatcgtc aatgaccttc tcggcggtaa tccaatttac gaatcattat tggaacgtct
     9361 acttggaaat actcagaccg cctcactaaa aggtgcaagg acaacttttg attttccagt
     9421 aacggcagag agttctttag acggattaat ggtacgagac ttcaattggc ctaaaaatat
     9481 gctgctaatc tctatacgca gaggaaatca ggaaatcttg actcacggtg atacgatcat
     9541 gaatgtagga gatattttgg ttatcttgac tgatgaagca aacttgccaa gaatcaagca
     9601 ggaaatcgag gctaaatctt ctatcacaaa tttgaagcaa tcattttaac gaacatggaa
     9661 aaactatttt acaacaacta ttcatctaca ctacttgatt ctatatgatt ctataaaaaa
     9721 agaagaggct gggaaaaccc agcctcttct tttttatttc tatgcttctg ctactgcagg
     9781 agcttttctc aataaaccat aaatcacacc agatacgact gctccgatag caataaacac
     9841 caaatacatg accgggtgac tcagtaagaa tacaacgaaa atcccgccgt gaggagccag
     9901 taatttgatt ccgaaagctc cgactaatcc gccagttaaa gcagaaccaa taataaaact
     9961 tggaatggca cgaatcgggt cagctgctgc aaatggaatc gatccttccg tgacaaacga
    10021 tagtcccatc acgatgttcg tcaaaccagc atcttgatct gttttgctga atttattctt
    10081 aaacaaacgt gtagcaacga aaattgccaa tggaggtacc attcctccag ccataacaga
    10141 agccatcacg acacttccgc cagttgcaac agtcgtagct aaagttgctg taccaaacac
    10201 ataagcggct ttgttgattg gaccgcctaa atctgccgcc atcattccag ctaataatgc
    10261 accaagtaaa gcagcattcg aaccactcat acttgtcaag aaaccgttca atgcatcatt
    10321 gattgctttc atagggacat tgaccagcaa catcaagaaa ccagtaatca gtaagccaaa
    10381 cactggataa aagaggatcg ttttaattcc atccaatgat tttggtaagt tcttgaataa
    10441 tttacgtaaa aatacgatga cataaccagc taagaatcca ccaatcagtg cacctaagaa
    10501 tcctgcaccg cctgtattag ctaatgcacc agcagcaaat ccaacgatca aaccagggcg
    10561 gtcaccgata cttgaagcaa taaatcctgc taataccggc agcatgaaac caaatgcagc
    10621 ttgtccgatt tggttgaacc agctagcagc ttgattgtaa ttccctagat ttgataattg
    10681 atcatgtggc acgccaataa attggtcgat catgaaagaa agcgcaatag cgattcctcc
    10741 accaataacg aaaggtaaca tgtgtgatac gccgttcatc aaatctttgt aaattctttg
    10801 tccgatagta ccatccgcat ttccttcttc tgaactgctt tcttttccat caccatggaa
    10861 tactggtgca gtaccactaa gggctagatt gatcaactct tctggtttac ggataccatc
    10921 actgacagga cgattgatca gttcttttcc gtcaaagcga ttcatctcta cttttttgtc
    10981 tgctgcaacg attacacctt ctgcacgggc gatgtcttca gcagtcaatc ggtttttgat
    11041 tccttctgaa ccgttcgttt cgactttgat ctccacaccc atttctttcg cttttttctt
    11101 caaagcatct tctgccatgt aagtgtgagc gatccctgtt ggacaagctg taacggcaac
    11161 aacgaatttt ctttctggat gagcagaagc ttctgcagct tgtgtttctt gttctgcttt
    11221 ttcttctgct tctttttgct cttcagctgt ttggaacaag tcttggactt cttctggtgt
    11281 atttgcttct tttaattttc ctacgaaatc tgcatcaatc agcaatcgag atagtgctgc
    11341 taatgcttgc aaatgtgtgt cgtttgcgcc ttctggagct gcgatcatga aaaacagata
    11401 agttggctgt ccgtctaagg cttcataatc tactcctttt tggctttttg cgaataaaac
    11461 tgttgcttct ttaactgcac tgtttttcgc gtgaggcatg gcgattccat caccaagacc
    11521 tgtagaagtt tgtgcttcgc gggctagaat tccttcttta tatgtttcga tatcggaaat
    11581 ccggccggca tcaaacattt tttggaccat ttcatcgatt gcgccttttt tatcagtcgc
    11641 ttgcaaatcc atgatcatcg cttctttgac cattaaatcc ttgatattca tgattatcct
    11701 ccttgaaact aatcaatttt tgtgatttca acttctggca gcaactcatc aatcaattcg
    11761 cgagctgcca agtcatcaga aaaagctgtt gcgcttccgc aagcaactcc ccatttaaag
    11821 gcttcgattg gatctttcgt acggctgtaa gccccaataa agccagcaat cattgaatcc
    11881 cctgcaccta ctgaattttt caatgggcga actaatacat tcgttttata agttccttgt
    11941 tccgtgaaga gcaaagcacc gtctccagcc attgagatca gcacattctg tgccccttca
    12001 gctagtaatt ttttcccata cggaatgatt tcttctacag attggaaggt tgtgttgtaa
    12061 agctcagcta attcgtgatt gtttggtttg atcaacaacg gtttcttaac aagtgcattt
    12121 tttaggtcat cacctgttgt atcaatcacg aaatctgcac ctttttctcg aatgatatca
    12181 atcagttctt gataaaatcc ttttcgcagg ctagctggcg cgcttcctga caatacgacg
    12241 atatctcctt gtcctactgt tcctaatgct tgcttcaact catcgatttc ttcatctgaa
    12301 atagatgggc ctaagccatt gatctcagtt tcagtgtctg attttagttt gatattgatt
    12361 cttgtatctt cttttacggt tgtgaacaaa gactggattt gttctttttc aagccacttc
    12421 gaaataaatt cgccagtgaa ccctcctaaa aaccctagtg ctgtagattg agaatcgatc
    12481 cgtttcaaaa tacgggaaac attgatccct tttcctccag gtaacttgaa gtcttccttc
    12541 attcggttca aatcgcctag ttttaaccca tctacgcgta cgatataatc aattgatgga
    12601 ttaagtgtta ctgtgtaaat cattgtattg cctccttaat tgttgtcttt tcacggaatt
    12661 cattaacgaa ttctaatgga caacgctcgg ttatgatcgt ggctgcttcc aaaggtgcaa
    12721 cagaggtgaa ggacaactgg ttgaatttgg tatgatcaat caatacaaat gtctcttcgg
    12781 aatttttcat ggctaaacgt ttaacagctg cctcttctgg atccggagtc gtgaatcctt
    12841 gttcaagatg ggcaccgttc attcccataa atgctttatt aaaatgaaaa ttttttagtt
    12901 gttctatcgc attagatcct aaaacagcat tagttgccag ctttatcgtt ccacctaaaa
    12961 taatggtagc aagctccaaa tcaactaaat ttgccgcatg ttgaacggaa tttgtcacta
    13021 ctgtgatctg ttttccttgc agaaaaggaa tcatttctaa cgtagtagag ccagcgtcta
    13081 aataaatgac atcttcgtca ttaatggttt cagctgccga tttggcgatt tgctgttttt
    13141 cgtgaacgtt tttgagtgat ttttcactca tattctgctc aaaattcaat aattgctctt
    13201 tcttagctcc gccatgaact ctttctagaa acccactatt ctccagctct tgtaaatcac
    13261 gtcttatcgt tgattcagaa gcattcaaaa ggtttacaag atcttgtgat ttcacgattt
    13321 tctgctgttc aagcaagtct aggatttttt gtctgcgttc ctctgtaagc attttcacga
    13381 tcacctcaat acaaataata gcatattttt tttctactag caatataaat cagtcaaaaa
    13441 cgttcataaa aattataaat cagtcaaaaa aaaataaaaa accagtcaga aagactggtt
    13501 ttttattttt ttgtctatca tcaagccgtt ttatcgtttt ttattttcgc ttcttgtttt
    13561 tttcatataa acagaagcag gttttctttc cttatgaaga agataataat aaagtcccat
    13621 cccgccaaac atgaccaaaa aaccgatgac tgatcgaata ggaaaattag gaacaaaaaa
    13681 gagacagacg gataataatg atgccggaac cattattcga taatagagtt gtttatccat
    13741 tttcttcacc tcatattttt tgtaaagtaa tcaattgttc taattctcgg taatctaaaa
    13801 attgcacacg cagatgctgc tcacctttct tgacatcgaa ttggatactt cgccgctttt
    13861 tctttattcg tttgaaggta acatcagaga gtctatgttt gatttccttt aacggtaatt
    13921 ctgacaggga ttctgaagga acatagattc ttaaggataa aagatactct tgatattttt
    13981 catctgttac ctctacttct agatattccc taatataata acaataagct tcgattccat
    14041 tatccaaagc aacagtcgga tcgataactt tatagcacat catgcgtgat cgattctcgt
    14101 caacccaagg aaaatacgcc tgagcattta gtgttccgcg aacacgcctt tctttataat
    14161 ctccttgttg gatatcataa ctttttgtta caaaaaatga agcattcttt ttaggaacag
    14221 gttgcatttg tgcattctga tactggacag attggatcgt atggtcagtg taaatgtatt
    14281 caacgaatgg cggtttcaat aaaccgacac cattgtccca agcaagatga tcaaacccaa
    14341 gctggtccct aaaataggat tgaaacattt gattgtttgg cttttgccag ttaaaggggt
    14401 ctcgataaag ctggatacat atgacttcgt tttctaattc cttcactttt tctacttgtt
    14461 ggatgtcgag aaaaatcggt ttagggatga tttgatgata atagcgacta taatacattt
    14521 cccaacacga agtgaaacat aaaccttggt aaaaaagatc gtatcctgga aattggttgc
    14581 aatcgactac tacttccccg ttttgttgtt tcattttagg taaatgttcg atttcatcct
    14641 ttttctcaaa caatagccgt tgagagatct gttttgtatt gtgatagata aactcgctat
    14701 aagctcgcat ataggcgaac accccatgag caatcatttt tttctgaatg aagtctcgaa
    14761 tcaaaaattg cttttctttg aaaacagaga tagacaatac attttttatc aataattgat
    14821 tatcttccaa gcgaaaacta cattcgttct cgtcatttcc tacaactaac caactttcgt
    14881 ctcttaccga tgctagcatt attttttctt gaagctttcg aagatcatac ggatccgctt
    14941 gttgtgtgaa agagcggata aatgttggct ggatcaacag acgtgtcaaa atagcccaat
    15001 cgcttatttc ctgttttttt tcttcttcat ttaagtctgt gagctggata gtcatcaata
    15061 atttatcttc gccaatcatt ttctcacctc tttcaaatct ttttataatt taattataac
    15121 caatgataaa gaaaaactaa aactttatgt cttagaaacc aatttggacg attacatgat
    15181 agactaagta aaaagaaaca aagaggtgtt tacaatgggg ttgaaatttg aacgtacaga
    15241 taatttagat aaacttttcg aatcatttgc tgtcgatccg gaaaaaacag ttgcaccaaa
    15301 agaagagatc gatcgatttc taaaaccaca gccacaaaat gaaaagaaaa aagacaagaa
    15361 acaaaagtaa gttaagctca aaaaaagact taccagccaa acttttatga aagagtttga
    15421 ctggtaagtc ttttttattc tttttatgat tcaattgctt gttcgattgc tgcccacgct
    15481 tcttcttttc cctgttttgt aacggaagaa aagagaataa acgtgtcatt gcgatcaaaa
    15541 tccaacgctt ttttgatttc gctctcatgc ttgttccatt taccacgagg gatcttatct
    15601 gccttagtgg caacaacaat tacagggata tcataatact tcaaaaattg atacatctgg
    15661 atatcttctt gagaaggctt atgacgcaaa tctactaaag aaacgaccgc acgtagctgg
    15721 tctctagtcg ttaaatatgt ctcgatcatc tttccccatt ttgctcgctc agttttggat
    15781 acttttgcat acccatatcc cggaacatcg acaaaatgta atgcgttctc gatcaaataa
    15841 aaattcaatg tctgggtctt tcctggctta ccggaagtac gggctaaatt ctttcgatca
    15901 atcaatgtgt tgataaaaga agatttccct acatttgatc gtcctgccag agctatttct
    15961 ggcaattgtg tatctggata ttgttttggt gccactgcac ttatgataat ttcagcttga
    16021 tggactttca tggtctctct ccttaacaag gtgatgaata aagcaagcgg ctgtcgcaaa
    16081 agccaaaatg acttttacgg cagcctcttt tcatcaaccc gcttttttct ctgaatcttt
    16141 atagattact gttggctcac cagacaattc agctgcttct tttgtgataa tcactttttc
    16201 gatgttttca tttgaaggga tatcaaacat cacatccatc atgatttctt caatgatgga
    16261 acgaagtcct cgagcaccgg tattgcgttc gattgctttt ttcgcaatgg ctctcaacgc
    16321 ttcaggttcg aattcaagtt cggtattatc tagagaaaga agtttttgat attgtttgac
    16381 tagcgcattt ttcggctcag tcaagatccg taccagatca tctgttgtca atttctctaa
    16441 agctgccata acaggtaatc gtccgataaa ctcagggatc aatccgaatt ttagtaaatc
    16501 ttcaggaatg atatgctgca tgacactttc gtcttcagaa agtttttggt tttgtgtacc
    16561 aaatccaatc gtcttttcgc ccatacgatt cttcacaatc gtttcgatgc catcaaatgc
    16621 accacctacg atgaacaata tgttcgtcgt atcaatctga ataaattctt gatgtggatg
    16681 tttacgtccg ccctgaggtg gcacacttgc aacagttcct tctagaattt tcagcaaggc
    16741 ttgttggaca ccttcgccgg aaacgtcacg agtaatagat acattttcgc ttttacgcgc
    16801 aattttatcg atctcatcaa tatagatgat ccctttttct gcaagttcca cattgtaatc
    16861 agcagattga agcaatttaa gcaaaatgtt ttctacatct tcccccacat aaccagcttc
    16921 tgtcaaactt gtcgcatctg cgattgcaaa aggtacattc aatgtttttg ctaatgtttg
    16981 tgctaaaaat gttttaccag accctgtagg tccgatcaag cagatattgc ttttttgcag
    17041 ttcaacatca tctgtgtttt cttgtgcttg ctgattgaca cgtttatagt gattgtatac
    17101 ggcaacagct aacgagcgtt ttgcgcgatc ttgtccaatc acgtagttat tcaatacttc
    17161 aagaatttct agtggtttag ggacttcagt gaattcgcgt acagcttctt cataaaattc
    17221 ttcatcaatg atttctttac ataggtcgat acattcatta cagatgtaga cccctggacc
    17281 agctacgatc tttttcactt cttcttgggt tttcccgcaa aaagaacagc gtacggtctc
    17341 attgctactt ggattgtcat acatggtctt caccccttaa cttgcaaaaa gtatgcaaga
    17401 aactatcaaa gactttacag tcttttacta tcataacata gtttagtaaa aagaaaaagt
    17461 gtttaacaaa ggatcaactg acctatacct ttgcattttt tactcttaac aaaaaataag
    17521 gaactgtgac aaaatcgtgt cacagtccct tatttcgaat aaacaatttt ctagaagtag
    17581 cttcttcaga aatgatttat tacttgattt ctattctttc gcttctgtcg ctgtacctgt
    17641 gatcaattcg attgcttttt tcattgtcac atcatgttcc agcatatctt ctgtcaacac
    17701 gcgtttgatt tgttcgattg gcatgttgta tgtttctgat aattcttgga tttctgcatt
    17761 gatttcttct tctgttgctt gaagattttc agcagctgca attgcttcga tcacaagatt
    17821 tgttcttgtg cgcatttctg cttcaccttc gaattgttta tgcaagtctt cttctgtaga
    17881 accagttaat tgatagtaca tttcaggaga gataccttga cgttgcatgt tgttcaagaa
    17941 ttcgtccatt gaacgatgta cttcatcatg aaccatcacg tgtggcaatt caacgatttc
    18001 tgcgttttca acagccatac ggatcgctgc ttcatctttt gcatcgtccg ctgctttttc
    18061 ttttgtttct actaattctt tgcgatattt ttctttcaat tcgtctaatg ttgatacttc
    18121 gtcatcaaca tcttttgcga attcgtcatc taattcaggc acttcttttg cttttacttc
    18181 atggattgtc actttgaaga ctgcttcttt tccagcaaga tcttctgctt gataatcttc
    18241 tgggaaagtt acatttacat ctagactttc acctgattta tgaccaacta attgatcttc
    18301 aaaacctgga atgaatgagc ctgaacctaa ttcaagtgag taattttcgc cttttccgcc
    18361 atcaaacgct tcgccatcta caaagccttc gaagtcgatt actactgtat cgccattttc
    18421 agctgcagca tcttctttga taactaattc agcttgtgct tcttgttcac gtttgatacg
    18481 tgcatccacg tcagcatctg ttacttcacg gtcttgtttt tcaacaacta gatctttata
    18541 ttgtcctaat tttacttctg gtttcactgt tacttcagca gtgattaccc agtcttgacc
    18601 tttttccatg ctttcaacat cgatttttgg ttgagaaaca ggatcgatgc cagcttcttt
    18661 gacagcagct tcataagctt ctggtaatac agcattcaat gcatcttcat ataaagcttc
    18721 ttcaccgtac atacggttga atacttgacg agaaactttc cctttacgga atccaggtac
    18781 gttcaagctt tttttcactt tattaaacgc agttgttaat cctttttgga tttcgtcttg
    18841 cgcaatcgta aattttaaaa caccatcatt cgtgcctttt ttttcaaatg ttgcagtcat
    18901 ttaattccct ccgagtttag aaattcattt tatacatcaa aaacgtttca tgcatacctt
    18961 taaatagtac actaaccctt tcaaattgta aacatattga atggatagaa tccttgtttt
    19021 tcataacatt tttatctttt tcactgataa aatctaatgg aagaagatca tttgctgcaa
    19081 aattttttct ctttttttta aaaaatcttg tttttcatct tctgtacctt catacatcgt
    19141 ttttcctgta cacgcctcta cagtcagttg aaaccagcgc ttcgggtctt cgatgtatgt
    19201 atcatagaca ggataaacaa ttgcagattg caacgtgaat tcttctttta acacggaact
    19261 aagcagcgca tcttcattcc cgtaacgatc tgaaaataca cataattgct ggtaacttgc
    19321 ctgctgctgc ggctttggca gatcttttgg tatgactttg actaactttt cttcaatcgt
    19381 caaatagaaa acaggatggc attcgttcaa ttgcctaagt gtctccagta attttgccct
    19441 tgctaaaata tggacctctg gcgatacaat aagtttactc gctgtttgga taaactcatg
    19501 ataaggaagc tgttcgatct catcaataga cgcaagctga cgtgcaggtt ccaacgaagg
    19561 aagttcctcc gatttattgt ggagtgcctt gatcttctgc ctctccattt gtccggataa
    19621 ttcttctgca aattggattt tttcaatgac ccgctgctcg aaagcttcat aaaaatctgt
    19681 acttgctatc aattttctag catataaaaa atcttgtgta ctgattaaca agtcaaaata
    19741 aaattcagca aactcctcat tttgaagata ctgtttttca tgcaagagtg cttgcttcaa
    19801 tgcttctttt ttctcgtcca actcgtaaag acaaaaaaca agcaggctat tagcctcaaa
    19861 gctttctttc aactcgtaag ctcgttgaaa taattctttt gcctgctgat attgctggtt
    19921 tgctagtgct tgttgtgcca ctcgcattaa attctcatat ttttctggaa aatcaatcgg
    19981 cttcacgcag tgtcctcctc ttgctcatat tcagctcatg ttcaatagta gcaaaacaaa
    20041 aaaagcatga caattaaaaa taactgaagc accctctaaa agctagattt tagatctgac
    20101 ttttagaagg tgctttcaac tgttactgct ttttattgct catcagctgt tgacttcctg
    20161 gttgaacgct ttgatcagat tgatcgtaac aacacttgta ttagtgaaaa aatcgcccag
    20221 atgttgcttt ttctgattaa agactgctgg aagataccct aacatcagca aaggaaattt
    20281 tagaatgaac cggcaagcac cttctctaac taaaacggtt gaccagctaa gttcttcttc
    20341 atcaaagctg atgactcgaa taccaaagat cattttccca atcatttggc catgattgaa
    20401 tttcgtcaat aaaataaaat acgctagata aataagtaag gctgtgaatc cgtagagact
    20461 cagataagaa tctgaaaaat taagccaacc tagatttttg gcaataccaa gtgttaaacg
    20521 cgtaatagct ccgatacaca aaagatcgac caagaaggca aacattcgga taaaaaaacc
    20581 tgcatagaaa tacttaggaa aatcatttgc ttttggcggt tcatttcttg tgtccgtata
    20641 ctttttccat ttacgttgtt tctttttgat ttcttcgggc gtcaaaggct ctctttcaaa
    20701 agagctcagt accttttggg aaaagttgga gtcaggtcct acagcaggct gctgttgctt
    20761 atctttttct tgttccttat tttcatttga aagctctgac atgcttactc acctccgtaa
    20821 taatacattg cttttggtga ttccgctgtc cctagattct caagaacgga taagatctga
    20881 ctagtctcag aaggcttcaa tccctggaat tctgctagtt tacttcctaa ccatgtacta
    20941 gcaaacccag atggcgtgga agaatattct actagttccg catcttcaag ctgttgttcc
    21001 tttcgtatag cttttaaagc atcttcagga aatcccaatt catcgactaa acctacttct
    21061 tttgcttgga caccatcata aatccttcca tcagcaattt ttttgacttc ttcttcagat
    21121 ttattccgtc cctcgctgac gattgctaca aaacgactat atgcgctgtc gatatatgct
    21181 tgaagcaccg cttcttcttc ttttgtctct ggtcgcgttc ctgaacccat atctttcaac
    21241 gctccgcttt taactgttgt atcttccaca cccagttttt ctagaagtcc tgaataattc
    21301 aatcctgaca tgatcacacc aattgagccg gtcactgttt cttctgttgc aaagatttta
    21361 tctgcttgag cagaaatata gtagccaccg ctagctgcca tgtttttcat gctgacatac
    21421 attgggatat catgttcttt acgaatcgta tccaatttct tagcgatttc cgcactttcg
    21481 tagacaccgc caccaggaga atttacttct aaaaagatac ctttgactgt tggatcttct
    21541 tcaatcgcct tcagttgttc cataaaattt ttatgattgt aaccttcaga agcaaataaa
    21601 cctgaagacc ctcccgcagc aatcgttcca tctactgata attttacgat tttttgcgta
    21661 ctgtctccct cttccaacac atttggagct agttcttcat ccccatataa ccaactgttc
    21721 aatccctcaa tttgatcgtt ttcctgaggc ttagtcaaac tagctgaaac taacgaaacc
    21781 acaaataatc ctgcagcaat cagaactgct gcccaacgtc ttttattcat tctttctcct
    21841 ttctaataca agttttttat gacgatcaca gtttctgact gctcagaagt gtcgaaccgc
    21901 tctgcttttt cttcccgcgc atacccggaa aacgactctt ttgttaattc atcttctacc
    21961 gtaaactcac gagcttgagc aacttccaag gcatcaggtg cgatttcttg gatgctatcc
    22021 cagccagtat ctaaaaatat tttcgtatca tctgatacac cagctgatgc ttcgaatttg
    22081 gacaattgtt tttttaacat tcccagtgtc atgatctcaa tttttgaatc catttttact
    22141 cctcctctat ccgattactt tctattgtac cgaactcctg aaaaagatga tagataaaaa
    22201 cccattattt tttgacataa aaaggacatt tgacaaagaa tttgatatat accaatttat
    22261 tttttattct ttttttgttg acaagaacat tcgcaaaagg ttatgatttt aaatgtttcc
    22321 agataaaaaa caaaaaggag gatagttata ccttttagga tagcgccagg tctgtgtaaa
    22381 cagatgacga ggagagggtt tatcgaaaga ttcggcggat ggccctaggg actgcactct
    22441 acagaaaatc cacaaaaaat gtctgcaaag ataaaacaaa aggattttcc tgcagctgga
    22501 agcattcata cgaattcatt atagaaagta ggatgttttt tatgtgcgga attgttggaa
    22561 tgattggatt aaaaaatgta acaccaggat tgattgacgg tctagaaaaa ttagaatacc
    22621 gtggttatga ttcagctggt atctttgtct cagatggcac aactgattat ctggtcaaag
    22681 cacaaggcag aattcaaaat ttgaaaaaca aaataaatat tgatactaca ggaaatatag
    22741 gtattggtca tacacgatgg gcaactcacg ggcaaccttc tgaagaaaac gcccatccac
    22801 atacttccca aagtggacgc ttcgtgctcg ttcacaacgg cgtaatcgaa aactttgaag
    22861 aacttaaaat tgcttattta gcaaatgacc actttattgg tgagaccgat actgagatta
    22921 tcgctcatct aattgaaact ttcgcgaagg atactacaac gcaagaggcg tttttaaaag
    22981 ctttgcgggt catcaaaggt tcttacgctt tcgctttgat tgatcggaca gcacctgatg
    23041 ttatttacgt cgctaaaaat aaaagtccac tattaatcgg actcggcgat ggattcaatg
    23101 tgattgccag cgatgcaatg gctatgcttg ctcatacgaa ggaattcgtt gagattgaag
    23161 atgaagagat ggttaccgtc acatcagaaa aagttattat tcaaaatttt gcaggtgaca
    23221 tcgtagaacg ttcttcattc gaagcacaag tagatgcttc agatatcgag aaaggtacct
    23281 atccttttta tatgctgaaa gaaatcgacg aacagcctat tatcatgcgt cgtatcgctc
    23341 aaaaatacgt aacagaagac aatcgtgtct ctcttgatca aaagctggtc gatacattgt
    23401 ctgatagcga tcgaatctat atcgttgctt gcggaactag ttaccatgcg gggttagcag
    23461 gtaaacagac tttggaaaaa ttgacacaga ttcctgttga ggttcattta gccagtgaat
    23521 ttggctataa cacacctctt ctttcaaaaa aaccattctt tattttcttg agtcaaagtg
    23581 gcgaaacagc agacagccgc caagtgctgg taaaaatcaa ccgtctcggc tacccttcac
    23641 ttacgattac aaatgttgct ggttctactc tttcgcggga agcatccttt acactgctat
    23701 tgcatgctgg acccgaaatc gcagtagcat caacgaaagc ttatactgca caaatagctg
    23761 tactaacttt attagccaaa gctatcggtg ataaaaaaga atttgaaact tctcttgtat
    23821 tcgatatcac tcatgaattg agtttagttg ctaatgcgat ggaatcggtc atcgcccaaa
    23881 aagattattt agaagagttg gcagaagtat atctcagcga tacaagaaat gccttttaca
    23941 ttggacgtgg tgctgactac aacgtttctc ttgaagctgc tttgaaattg aaagaaatca
    24001 gttatatcca ggcagaaggt tttgccgctg gcgaattgaa acatggaacg attgctttga
    24061 tcgaagaagg aacaccagtg atcggaatca tcagtgaaga agtgactggt gcgcatactc
    24121 gaggaaacct gaaagaagtc gaaagccgtg gcgctaaaac actagttatt gtctcagagg
    24181 gattagaaaa ggaaagtgac cagctggtgc taccaactgt ccacccttat ctaactactc
    24241 taaccacagt cgtaccgact caattacttg catattacgc aacgctatta cggggattcg
    24301 acgttgacaa accacgtaat ttagcgaaat cagtgacagt agaataataa aagaacaagg
    24361 accagtgtca gacatctgac actggtcctt gttcttttat tttttatgaa aatatctctt
    24421 aatccagtcc gatttcttct ttgactactg ccgcgatttt atcgacatag taattaactt
    24481 tctcatcagt aggcgcttct gccatcactc gaagtaatgg ttctgtaccg gaaggacgaa
    24541 ctaggattct tccttctgat cccatttcag cttctgcctc ttcaatcact tttttgattg
    24601 ccggaacatc catcgcaccg tttttatcac ttacgcggat attcactaat ttttgcggat
    24661 agattgttac ctctgctgca agctctgata atttcttacc tgtctgtttc atgatattca
    24721 gtaactgaat tcctgaaagc atcccatctc cagttgtgtt aaaatctaag aaaatcatgt
    24781 gcccagactg ctcgccgcca aagttataat cgtttttgcg catttcttct acaacgtaac
    24841 gatcaccaac ttgtgtaacg acatctttca aaccgattcc ttccaccgct ttgtggaatc
    24901 ctaagttact catgacagtc gtaacgatcg tatctttttt caatcgattt ttttctgcca
    24961 agtatttagc acagataaac atgattttat caccgtcgac aatgtttcca agttcatcca
    25021 ccgcaataat acgatcgcca tcgccatcga aagctagtcc agcatctgct cctttttcaa
    25081 cgaccatctc agctaaacgt tctggatggg tagagcctac gccgtcattg atatttaacc
    25141 cgtctggatt tgtgcccatt gtataaaaat ctgtttcaag atcggcaaac aaacgattaa
    25201 cacttgttga agttgctccg tttgctgcat cgacacaaat ggtcaaaccg gaaaggtcac
    25261 cgttgatcgt ttgtacaagg aattgagaat atttcaataa gccttctgga aattcttcta
    25321 cagttcccaa tccttcagct gatggacgag gcaattcatc cacttcagca tctaataatg
    25381 cttcaatttc tagctcttgg tcatctacta gtttaaaacc atctgcaccg aaaaatttga
    25441 ttccattatc ttgtgctgga ttatgagaag cagaaatcat gacccctgcg cttgcttttt
    25501 gcaaacgtgt cagataagca acacctggtg tagagatcac acctagctgg aacacttcga
    25561 tacccaccga taaaagtcct gaaatcagcg cttcttctaa catttgacct gagattcttg
    25621 tatcccgccc tactaacacg cgtgggcgtt ttccttcttc ttcatgctgg cttaggacat
    25681 aacctccgca ccgtcccagc ttaaatgcaa gttctggcgt aagttccttg ttggcttcac
    25741 cgcgtacgcc gtctgttcca aaatatttac ccatagttgt aaaattactc ctttaactta
    25801 attttctgag gaatttgttc tgctcatttg tgttgattcg cttgtttctg atgtttcttc
    25861 tgtacttgtg gtcgagctgc ttgtcccaga ttccgaactg gaagaaatgg ttgttgtcga
    25921 tggttccgtt cctgaatcag cagatgaaat acttctactc gtacttgtat tttcttgtgt
    25981 ccgactgctc gtcgaagcag agctactgct tgacgtttgc aacacgggtg taatctccac
    26041 actaactgat tcgggcttga tacttaaccc ctcttctact ggtatttcaa ttttacgagt
    26101 tgttcgatga ttgatacctg agacattgat cggtacagca atactgtcac cgatcgatgc
    26161 aagcaaatcg ctagagccgc ttacttctgc ttccagtttt gaaaaactga aatcatagct
    26221 ttctaccccg gcagctggtg ttccttgctg tatcccataa agtcgtaccg acttgcttgg
    26281 cttgatcgaa tcagctctca ctgttacttg cactggatca gagacgatac ttaatacttc
    26341 ccctgctgca ttcagcgcct gtattgggac ctcactatta atcccatcat ctgtgaccat
    26401 cgaagcgtcc acatcagcta ctactcgttt gatttctgcc aatgttttgt cacctgtagt
    26461 gatagagact tttttaggac taacggaagt atcatcaagt tcatatccag atggtgtgaa
    26521 cgttgaagga atcaacgttt ccacatcgaa atcttttatt acctttttct caaccgttac
    26581 tgtaatagtc gatggttcga tctttgctgt gacagcactg ctcaaatttt gaatttttag
    26641 cggaacttca tgtgttcctt cgcccagctt agtgatatct gctgtgacgc ggaacatccg
    26701 tgtatcttcg tttgcctctg cattcaactg aactcggttt gcactggaaa gtttgacagt
    26761 cactgcattt tcatatccat ggatatagta gcgctttgaa tcataagaaa tcttgatcgg
    26821 cacccccgta actgtttgat tatacgcttc atttccagaa agcgtatttg gaacggactg
    26881 gccatttgca ttgaagtaca gcgcaatact gaacagcaat gcaataaaac tgtaaagaat
    26941 gcttgtccgt ttctcttttg agatcatttg cgatcccctc ccttagtcat accgtctagg
    27001 aaagattgaa tcacgttttt cttgtttttt gttttctctt cagcaattag ttctctacgc
    27061 aagattgcca aatattcttg ctgggataag ccagccatca gttcattatc taacgtgatg
    27121 ctgacgtccc cggtttcttc agagacaacg atggtaatcg cgtcactgac ttcgcttata
    27181 ccgacagctg cccggtgtcg agtcccgaat tctttaggga tcaacatact ttctgataga
    27241 ggaagatagg cgcttgctac tgcgatcttt ccttctttta caataactgc accgtcatgc
    27301 agcggtgtat taggaataaa gatattgatc agcagctccc cagtgatatc tgcatctaaa
    27361 gcaatccctg tctcaatata ctcatctaat ccagtatgac gttcgatagt gatcaacgca
    27421 ccgatttttc gcttggacat atattggatg gctttgtcaa aagacaagat catcttctca
    27481 tcttcctgct gttcgctttt tgattgtttg aaaagcgaac tgcgtccaag atgttccagt
    27541 cctctccgga tttccggctg aaaaatcacg atagcagcta tcaccccgta cgtaatgact
    27601 tggttcatca gccaagacaa ggtgtgcaga ccaatgattt ccgctacgat tcggataaca
    27661 ataaaaatag ctacaccttt cagaagctga atcgcttttg tgcctcgcag cagttggatc
    27721 agtttgtata caagatacca gacaaccaga atatcaagta tgttcaccaa aaaattcaga
    27781 gagaacaagt caccggaaaa catttgctgc caatagtcca aattaaaaag ttccccgatt
    27841 tgaaatgaca taaaaagcct ctcttctctt ttgagatttc ctctctagta tatcatatgt
    27901 tttaatttta gaggctgata tgctcgtatt tttcttgaga aaaacgcata attttcaaga
    27961 aaattgcaag tatgcgtttt atttttctct gcaataaata tttttaaact tcagagaatt
    28021 attcattata ttttttctgt tatttttcct ttttttcttt tgtagcttgt tccatttttt
    28081 tacgtcgttc tgctctggca gctcttctag cagctcttct ttcttctgct tcaaggtccg
    28141 tcgttttttc ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt
    28201 tcttgatttt ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt
    28261 cgtggacagt cttttcaaat aaagcttttg caagatttgg atttgcgtta tctagcgagg
    28321 aaaagcgaac ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt
    28381 ccaaagtcat tgggttcttg tttttttcga ccattgtagg gttatatcga tataatgacc
    28441 aataaccaga agaaacagct tcttttgctt ctttaagcga ttctttcatt ccgccaagca
    28501 agccatgtgt gatacaaggc gtataggcaa taatcagaga aggaccagga tacttttctg
    28561 cttcttcaaa tgccttgatc gtttgcattt gattggcacc tgaagcaatt tgcgccacat
    28621 aaacatttcc atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac
    28681 tagccgaaaa tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat
    28741 tggaatagac ttcgttatcc aaaacaagga tattcacgtc tgctccgctg gcaagtacat
    28801 gatcgatccc gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc
    28861 tgttttttac gaatagatcc tttctcttgt atatatcctc cagttcaggg aaaattgatc
    28921 gttcgctttc gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcactctcaa
    28981 acatgtgtac taaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata
    29041 cttctttgat tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat
    29101 aaccaaattc agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt
    29161 cattggtcgt ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg
    29221 cattcgcgat catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt
    29281 cgccacagcc tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca
    29341 agacagtatt ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc
    29401 aattcacagc ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg
    29461 cgggacaagc ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac
    29521 ggtattttac gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt
    29581 ccatttcctc ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac
    29641 attcattaca catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc
    29701 gcttttccat agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa
    29761 gtgtaccaac agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta
    29821 tataaccagt gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg
    29881 ggatcgcgac ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta
    29941 cgatagccat tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca
    30001 cctcatcaaa aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga
    30061 tccggcgtcc caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa
    30121 tgttattttc agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt
    30181 cgctccaaag agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat
    30241 agctatgcag gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg
    30301 ctgaacgtat cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt
    30361 tcgaatcata ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga
    30421 tagctgattt atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga
    30481 atgtttgcgg ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat
    30541 catccacgat ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat
    30601 caaaaactgc tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac
    30661 caataacagc cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag
    30721 gttctccacc tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat
    30781 gaggcaactt ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa
    30841 aacctgtttt ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa
    30901 ctgatcccat agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt
    30961 cataagaagt tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta
    31021 atttatcata ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt
    31081 tcgtaccact aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt
    31141 tctcctgatt gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct
    31201 cgtgactcgt acggaatcca tcaaagaaat tcaaaaaagg gacttctcct tcaatagaag
    31261 caagatgggc aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag
    31321 caaatcctgt ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat
    31381 ttgtcgtcac tgcccgacta gcaacgtgga aaacacttgg cagtagttcc ccagcaatct
    31441 tgtacatatt cggtatcatc aaaagcagcc cttgtgaagc ggtatatgta gttgtcagtg
    31501 caccagtttt caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta
    31561 ctacttttac tggttgaccg aatacgtttt cttttcctgc tgctgaccat tgatctacta
    31621 attcagccat ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat
    31681 aagaaatata tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc
    31741 atccttgttc atattctctc ttattatctg ttccattata acgtaaaaca tgccattttt
    31801 ttcttttatt tataaaaata ttttacatgt atctatttga attggtcatc taatttttag
    31861 acaaacagtc taaatcgttg cctttttttg tcagacatgt ataaactata agcgaaagag
    31921 gtgttttaac atggatatat cagtgattga tgcaacgaaa acaaatacac aaaaaggaat
    31981 cctttatggt tcatcgaatg ctcctaaaaa aatggtcgaa ttcatcaact tagcttgtcc
    32041 gtattgccga caatggtttg aagaatctta tgagctatta gaagaagcag tccaaagcgg
    32101 gcaattgcag cgagtgatca aattgttcga taaagaaaaa gaaagcttac agcgagggaa
    32161 cgtgatgcac cggtatctca caatcagcga tggccaaaaa gcgatcaaag agatcaaaca
    32221 aatattcgat actcaagacg agtggaaaca tctttctctc caagaagtag cggattttgc
    32281 agtagataaa ctaaagctga cagaacaaaa agacgaacag ctgagccaag ctgtcatcaa
    32341 cgaagctgag caagcacata ttcgatttgt tccaactgtc atcttgggta aagaaatctt
    32401 tgatgaatct atttcaatag aggaattaaa agaattgatc caaacaaaat aaaaaaataa
//
LOCUS       DACEPD010000020        32194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.020, whole genome shotgun sequence.
ACCESSION   DACEPD010000020 DACEPD010000000
VERSION     DACEPD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.020"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            161..490
                     /locus_tag="IW797_000954"
     CDS             161..490
                     /locus_tag="IW797_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223744.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            477..818
                     /locus_tag="IW797_000955"
     CDS             477..818
                     /locus_tag="IW797_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ1223745.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            932..1171
                     /locus_tag="IW797_000956"
     CDS             932..1171
                     /locus_tag="IW797_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223746.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            1238..1477
                     /locus_tag="IW797_000957"
     CDS             1238..1477
                     /locus_tag="IW797_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223747.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            1604..2293
                     /locus_tag="IW797_000958"
     CDS             1604..2293
                     /locus_tag="IW797_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAQ1223748.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            2299..3465
                     /locus_tag="IW797_000959"
     CDS             2299..3465
                     /locus_tag="IW797_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1223749.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            3547..4218
                     /locus_tag="IW797_000960"
     CDS             3547..4218
                     /locus_tag="IW797_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ1223750.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            4271..6247
                     /locus_tag="IW797_000961"
     CDS             4271..6247
                     /locus_tag="IW797_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1223751.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            6260..7012
                     /locus_tag="IW797_000962"
     CDS             6260..7012
                     /locus_tag="IW797_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1223752.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            7028..7789
                     /locus_tag="IW797_000963"
     CDS             7028..7789
                     /locus_tag="IW797_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223753.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            7802..8062
                     /locus_tag="IW797_000964"
     CDS             7802..8062
                     /locus_tag="IW797_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223754.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            8059..10149
                     /locus_tag="IW797_000965"
     CDS             8059..10149
                     /locus_tag="IW797_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1223755.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(10185..12242)
                     /locus_tag="IW797_000966"
     CDS             complement(10185..12242)
                     /locus_tag="IW797_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ1223756.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(12387..12578)
                     /locus_tag="IW797_000967"
     CDS             complement(12387..12578)
                     /locus_tag="IW797_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223757.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            12766..13221
                     /locus_tag="IW797_000968"
     CDS             12766..13221
                     /locus_tag="IW797_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223758.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            13218..13460
                     /locus_tag="IW797_000969"
     CDS             13218..13460
                     /locus_tag="IW797_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223759.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            13478..13780
                     /locus_tag="IW797_000970"
     CDS             13478..13780
                     /locus_tag="IW797_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223760.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            13849..15903
                     /locus_tag="IW797_000971"
     CDS             13849..15903
                     /locus_tag="IW797_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223761.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            15903..18515
                     /locus_tag="IW797_000972"
     CDS             15903..18515
                     /locus_tag="IW797_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ1223762.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            18512..20890
                     /locus_tag="IW797_000973"
     CDS             18512..20890
                     /locus_tag="IW797_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223763.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            20951..21493
                     /locus_tag="IW797_000974"
     CDS             20951..21493
                     /locus_tag="IW797_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223764.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            21571..21903
                     /locus_tag="IW797_000975"
     CDS             21571..21903
                     /locus_tag="IW797_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223765.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            21904..22497
                     /locus_tag="IW797_000976"
     CDS             21904..22497
                     /locus_tag="IW797_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223766.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            22519..24543
                     /locus_tag="IW797_000977"
     CDS             22519..24543
                     /locus_tag="IW797_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ1223767.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            24543..25742
                     /locus_tag="IW797_000978"
     CDS             24543..25742
                     /locus_tag="IW797_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1223768.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            25758..26402
                     /locus_tag="IW797_000979"
     CDS             25758..26402
                     /locus_tag="IW797_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223769.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            26413..27219
                     /locus_tag="IW797_000980"
     CDS             26413..27219
                     /locus_tag="IW797_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223770.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            27242..27471
                     /locus_tag="IW797_000981"
                     /pseudo
     CDS             27242..27471
                     /locus_tag="IW797_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27630..28565
                     /locus_tag="IW797_000982"
     CDS             27630..28565
                     /locus_tag="IW797_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAQ1223771.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            28609..28878
                     /locus_tag="IW797_000983"
     CDS             28609..28878
                     /locus_tag="IW797_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223772.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            29154..29429
                     /locus_tag="IW797_000984"
     CDS             29154..29429
                     /locus_tag="IW797_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223773.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            29471..29893
                     /locus_tag="IW797_000985"
     CDS             29471..29893
                     /locus_tag="IW797_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223774.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            29913..31505
                     /locus_tag="IW797_000986"
     CDS             29913..31505
                     /locus_tag="IW797_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223775.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            31526..31861
                     /locus_tag="IW797_000987"
     CDS             31526..31861
                     /locus_tag="IW797_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223776.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            32007..>32194
                     /locus_tag="IW797_000988"
     CDS             32007..>32194
                     /locus_tag="IW797_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223777.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT        11590 a         4964 c         6567 g         9073 t
ORIGIN      
        1 attggatcaa tcgagcattt accgctgtct actagggttt ctcgtttcgg acgccataaa
       61 cgagtcgatt tattttatat atttttgagt gaacgaaaaa cgataaagat attcgttatg
      121 aagtaaaact aatttagtag atagcaaagg tggtagagga atgattcaaa aatttgcgaa
      181 aatgagtaaa actgcatgga ttgaaaagct tgaaaatgac ttgagtgtca tgcgattagc
      241 tccaaagaaa ttcaaacgag agattgcaga aaaagaagca attttgtcgg tattgaatgc
      301 ggtagacgat aaatgtccag taaaaattag tttttcagat atagcaacaa ttatcgaagg
      361 tatttattat ggtgaagttg atgaagagat atcttctaca aaagatttat tagatttttt
      421 ggataaaaac aaagatacct ttatagtact aactgattat gaggaggtta caacaaatga
      481 aaaattatga tccgaatata cgattgggca cacatacgat taaggtttct ttccaacgtt
      541 gggattacaa aggatttgtg acgtttagaa gaggcggaaa ttgtaaagga ttagatgttt
      601 tagcattaga cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg
      661 gcttgctacc tgaagatgat gaaggtaatg aatggtttaa aatgacgctt acggatgata
      721 atggagacga attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagcg
      781 ttgaaatcat tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac
      841 agataaaggc ttagaaggag tcacgacgaa taataaaaag gtagtattac cttaggcatt
      901 gagtaaggaa tttatttagg aggaaaatta gatggcgcga ccagttttag aggcgatgga
      961 tatggatact ttgacaaaag gaaggtatgt acagctgtta gctcgtggtt attcacctaa
     1021 agatgtattc aaatccctgt ctaaaggaac agatatggaa aaagaaagat tacgaaaaga
     1081 attcgattat tggagaaccc ataatggaat caaggatttg aaaccagcac ggccagtaat
     1141 taaacgaaag aaaaagataa cccaagactg aagtgtagag cgtatgtgat cataggcttt
     1201 tttatttttg caaaaaacaa gaaaggtagg aaaactcatg aattatgaag aaatcgaaaa
     1261 cagaaaaaaa gtgtcgaaag aaatggaaga aaagttgcta aaaatgatga aacaaaaaca
     1321 ccttaaaaga ttatctgtca tgcaatatat caatgatatg aagataacag gaaaagagaa
     1381 agcttgtttg ttaggttcaa tgaaaaactt tgaacagttg aggcgaactt atgtaaaaac
     1441 aggttccaat tgccaactat tattagaagt ttcttgacga ttatgatatg aaaatgatca
     1501 attatgatgt ggcattgtta ttgaataaaa agaatagtaa aatttaacat ggtttatctt
     1561 ggttagatgt aggtaaatta attcatatat agaaggtaga tagatgaata aaaacaataa
     1621 aactttattt aatccttacg tgggcgtaat cattgttttt cttcttttta gttattttgc
     1681 ttatcaaggt tatcaaaagg acaaaatcgc tgaaaaagta gatggtgtaa caaaaacaat
     1741 ccatattcag gaacaggcta aaacctatca agataattta accagtttaa cggatatttc
     1801 taagagacaa ctgattacta atcaaaaaca ggcattagaa aaggatctca aaggtgaaat
     1861 tgctggaaaa cttgtttttt cttctacagg atttattctc cctgtttttc aaggagcgaa
     1921 tagcgacacg cttagtttag gagtagcttc tacttattat ctagatactg aaatgggaaa
     1981 tggaaattat attcttgctg gttataacgt agatacacca agcgttttat tatccgatat
     2041 gactgattta tctgaaggag aagaaattga tctaatcgat gctcattaca agtatcagta
     2101 tgaagtctcc aaaagaggaa aaatttctga ttacagccag gtagttcaat ctaaggaaaa
     2161 caattccttt ttatccttgc caaaagcagg taaaaaacca ctactcacac tgttagcctt
     2221 tcaagcaaat aatatgaaaa agtgcgatgt gatccagtgt gaattagtca atgtagaaaa
     2281 aatcaataaa taagggaagt gagtcacatt caagaacttg tagaaaaatt gatcaataaa
     2341 atgagtgcca tttttctgtt actgttggta tccattctat atttaatatg gacggtgggg
     2401 ttaaatcctc ctacgatatt atttatttat gataatgctg cacagttagt gacgtttttc
     2461 gtaggctgtt tgtttctttt gaatctgtac aaggtaaaga gaaacgatct attcttcatt
     2521 gcagcaggca ttctttttta ccttttcttt tcttttttta gaagctaccg aggagatact
     2581 ttctatggtg atctgatcgt tccgacaatt attgctatta ttttgattgt aaaatggacg
     2641 aaatttactg taattgatcg tagcttgtat cttgtagtat tctatgtctt tttactcgta
     2701 acggtttatc gagtatttac tgagatacca gtaccagaag gacaaagtat ttggaatcca
     2761 tcgaacaaat tatcagatat ttggatcaat acaaatacga taggtagttc tttaatgaca
     2821 ttagcccttc ttatcacagg atttgctagt tcatttggaa aatggtacat gcgattgcta
     2881 tcattaccag cgttgattct tgctggggca acgatttggg tttgccaatc gaaagcagca
     2941 ttatatgcgc ttgttatctt cattatctta gaaattttac ctaagaaggt ttttcgaaat
     3001 acacacttgt ccttttggat gtatgcagca gtttcggttc tagctttacc gatttcttat
     3061 tttgctgcaa tgtcagacaa agttcactta tttacaggac gagaagaaat ttggcgcaaa
     3121 ttctatgaaa cattaggcga aaaaacagga caaatatttg tagggatgaa acctttcttt
     3181 ttccatagag gaaatcagat tctaggtaat cataatacgt atcacgcact cgtcaatcac
     3241 tatggattga ttggttttgt tctatcgatt attcttttgc ttagtttctt atggttgctc
     3301 gtaagaaaac gtgagctgac aaatggacaa ctaacattct tgtgggcttt tttagcgatc
     3361 atgtgtcaat cgttcatgga agaaacatta acgacaatca gttgggtgcc aattgtattc
     3421 ttaatgttag caatggctac acaccaatat aaaaccacca gataaatgaa atagaatagt
     3481 aataagagaa aacacttttt cgttgtgaaa aagtgttttt tgttttgtct gaaaggaggc
     3541 agatggatga aaaaatggtt atttggtttt ttaggagtcg ctttaatcgt cgtttgcagt
     3601 gtgtttggct atgttagcta ccaaaaacat gaaggagagg tgtttaagca aaatatagaa
     3661 aaaaagatgc ccgtagatca aatcaatgca cacgcaaaga gttataagga ggatgcaacg
     3721 aacgtcaata atgatatgtc tttggggcaa atgttgtcca tacaaaaaga agcaatcgaa
     3781 atgggcgtca ataaacaagt atttgcacaa attcagattc ctgcacttgg tttagcattg
     3841 ccaattttta aaggggcaaa tcaatacact ttgagtcttg gagcggctac ttatttttat
     3901 gaagatgccg aaatgggtaa aggaaactat gtcctagcag gtcataacat ggaaatgcca
     3961 ggtgttttat tttctgatat tcaaaagttg tcattagggg aggtgatgga cttagttagt
     4021 aacgatggcg tctatcgtta caaggtaacg cgtaaattta tcgtacctga gtactttaaa
     4081 ttaatcgatg gcgttcctga agagaactct ttcttgtctt taccaaagaa aggagagaaa
     4141 cccttattaa cgttgtttac ttgtgtttat acgtcacaag gaaaagaaag atacgtggta
     4201 cagggagagc tacaataaca agaatttaat caacgcgagt cgttgaaata tgaaatgagg
     4261 agagtaaaaa atggcaataa atcggtatgt aaaaaaagct ggaatgattg ttggaatgtt
     4321 aggaatcatt gcaggctgct ttcaggggta tcgagcttat gcagaagatg tcacgcaaaa
     4381 aacgccacca gaaagggtca atatcacggt tcataaatta atgtacgatc aaagtacgca
     4441 attgaacgtt gatattgacg gaatcaaaaa tgatggttat acgcacgatc aatatcctac
     4501 aggtgtaacg aaatacaaca aggcagatta tggtgatgtt gagtttacgt taggaaatat
     4561 tacggatcaa gtattaccga ctgaagattc tgatctaacc aacgcgaaag tggacgaaat
     4621 cgttaaagat gtagaagaca agggatctaa cagtgagtac gttaagaatg caaccaataa
     4681 aattacttca gctgtagatg aaaatggtga gatcactttt gctgatcaac cagcctatgt
     4741 caattccaaa gggaatgtat atgtggttta tgaaagtaaa tccgctgctg gattagtcac
     4801 acaaaaagca aaaccaatgg ttgtgattgc gccaatgaca gacaacacag gatcaggatt
     4861 tttaaaagat attcatttgt atccaaagaa cattgtttct aagttgagtt ttgaattaac
     4921 gaaatttggt gacgatggca cagctcaaag caaacaaaca cctttaaaag gggcaaaatt
     4981 tgagttatac aagggcgaac ctggaaaagg aacaaaatta ggagagcttg tatcggatga
     5041 tcaaggaaag ctgactgcaa ctgatttgac gttaggaaaa tactactttg tggaagttcc
     5101 ttcagaagtt gtagttggat cagatcaaga accaacagca gaccaatatt tgttaggcgc
     5161 agacgctcgc aacgatgcgc acaataagtt aacctttgaa attacgaatg acggcgtgac
     5221 aagtgattta aaagcttcct acgtgaacta taaagcacca gtgatcgata aaacagtgac
     5281 aaatggaaca ggagaagaac attctttcca aattggtgac gcagtgaatt atcaaggtac
     5341 aattcatatc cctacagata tcgcaggtgg aacagatggg attacagtaa atggtgtaaa
     5401 aagtgaaact tctccttaca gtgtatttaa atggggcgac acagcaggta aaggactttc
     5461 ttatgttgct gaaaaagcag atattaaagt aacaaataat gatggttcag tcgtattaaa
     5521 agaaaatact gactataaaa tcgaaaattc tgatagtggt tttgtgatcg attttatcgt
     5581 aaataatgga caagtaagtg atacagttgc gaatttacat ggtcaagatc taaaaatgac
     5641 ttataacatg tttgtgaatg atagtgctga aatcgcaaat ccattaacaa atagcgtgga
     5701 tttcgtttat aacaataatc catttaatca ggaagaacac cacgaaaaaa cgaaagctga
     5761 cgttgtgact tatggcgcaa aattcttaaa agtcgatagc ggcctatttg gaacaggtat
     5821 caaagcaaca ccgcttgaag gcgcagaatt tgcagctaag aatgcagaag gtaagtacta
     5881 cggtggactt actgatacag ataaagatgg tgtcaaagaa gcagtttggg tagacgatgt
     5941 agcaaatgca gcaatcctaa aatcagataa agagggtcat tttgaaatca ctggtttaac
     6001 tgaaggagat tactcactgg aagaaacaaa agctcctgaa aactatcaaa aattaacaaa
     6061 agagatctct ttcaaagtag acaaagattc atacaaagaa gaaaaccgaa tcacgatcaa
     6121 aaataatcaa aaagcttccg taccaatgac tggtagcaac ggtttccaat cttacgtatt
     6181 gatctcttgc ttgttgcttg gggcaggcgc aatttctgca gttgtttact ttaagaagaa
     6241 agcctagaag gatcagctaa tgattatcag acaccctaag aaaaagcgaa tcatgggtaa
     6301 atggatcatt gctttctggt tactttcagc tgttggtgtc ttacttttga tgcctgctga
     6361 agcgagcgtg gcgaaatacc aacaaaatca gcagattgcc gcaatcgatc gaacaggaac
     6421 ggcagcagaa acggattctt ctttggatgt tgctaagatt gagttaggcg atcctgttgg
     6481 tattcttaca attccaagta tttctttaaa actaccaatt tatgacggaa cttccgacaa
     6541 aattttagag aatggtgtag ggattacgga aggaactggc gatattacag gaggaaacgg
     6601 taaaaatcct ttaatcgcag ggcacagtgg actttataaa gataatcttt tcgatgattt
     6661 gccttcagtt aaaaagggag aaaagtttta tatcaaagtt gatggtgagc agcacgctta
     6721 tcaaatcgat cggattgagg aagtgcaaaa agacgaacta caaagaaact ttgtcactta
     6781 cttagaacca aatcccaatg aagatcgagt aacgctaatg acatgtacgc ctaaggggat
     6841 caacacacac cgtttcctag tgtatgggaa gcgagtaacc tttacaaaat cagaattaaa
     6901 agatgaagaa aataagaaac aaaaattatc ttggaaatgg cttttaggaa gtactgtttt
     6961 tctttcagtc atgatcattg gttcgctatt tgtttataaa aagaaaaaat aaaggagggt
     7021 aaccaacatg agaaatagaa aatggctatt tgtttgtgca gccgctcttt tttctttcac
     7081 tgcctacaca cagttgacgt ttgcagacag acaagaaagc acggatagca cagaacaaac
     7141 aatccaaacg tcagatagca gcacgattca aagcagcgat gaaaccgtcg attcgtcaac
     7201 agttgattct tcagaagaac ctaaaattgc tgaagacgat caaatagtca aacagaccat
     7261 tcacgttcaa aaaattattt cttccgaaca attagacaac caggggcaaa agcttgaaaa
     7321 acaagaagga gtaaatggcg cgaagttttc tgtttatgat gtatcggaca ttttacaaaa
     7381 gatggatgtt aaggatctca ctactgacca aattgaatcc caattaaaag atcgagtgaa
     7441 aaaactatcc agtgatcaac tgaagctaat ttctaatgga gaaacaaaga caattgatca
     7501 acaaacaggt gtctttgaat tctcggtaga agtacaggca aatcagaaac aagcttatta
     7561 tattgtcaat gaatcaagtc ctgaaaacat ttctaatagc gaggatattt tgctattaac
     7621 acctgtttct gataaaaatg gtgaattctt aaaggatgtg tggatttatc ctaaatcaga
     7681 agcttcgcaa cctaaagaag aagtaaaaaa aatcgtatct actggcgtaa agaaaaactt
     7741 ctttgagaat tgttgggatt ttgttactca cttgttttct agagattaag gaagaaataa
     7801 aatgaaactg atcaaattat tattattcaa tttttcttgt ttaaccgcag tctttttgta
     7861 ctgcggttta ttattttcta aactatctat tttgccaagc taccaactat acctaatgag
     7921 gattttaaac ataccagctg cttccatgcc gctagatatt atggaaaagc tcattaggaa
     7981 tccattattt actacgatca atttgatcgt tattgtcgga atttcactag tcatttataa
     8041 aaaagagggg atgaagggat gaattttaaa cactcgtgga caagacggtt aaaatatgtt
     8101 tttcccataa tgatgatgtt aggtacattg tttgggttaa aaacagccaa tgtctttgct
     8161 gaaaaagtta ttgtcgatcc gtcaaatcca atccagaacg taaagaataa ttttattatt
     8221 ccaaaagatg ttcctaatgc aaaaacaaat attactgtaa acggagcgtc aaaagtttac
     8281 aaatatcatg ttgatgccaa caaaggtggt tttacagacg actacgtagg attagtggag
     8341 ggaaacaaaa atatacgcta tccttctttc caaaaagaat acggtgaaac aaaccttgtt
     8401 ttagaaggcg taacaaccaa caccaaagtc aatattgact atggaaaaat cggtacgtat
     8461 aacgggaaaa aagtcaatat caagctagtt ctatctaata ttcacttata ctcggatacg
     8521 ttgccgtgga atctactaga taacaactat acaaaaactc attttcgaga cgatggctat
     8581 aaaaatacga atggtgcaat gagtaaatct aaaaaaagaa cagtactttg gatctcagac
     8641 aatcttttct caggaattgt ttaccattca acacaaatga atgtgcagct agtagccaca
     8701 tatgaggacg gtagccccgt tcaattttca ggagatacat ttatcagttt taactctttg
     8761 aaccctgcag gaggaaaaag cacggatctt aaaggagaat atgcccacta tgacaaaatg
     8821 aatacgacag attggtatgt cagaaaagat acggttcttt cagaatttaa atctttctac
     8881 aacaatctga acgtggtagg tggccaccca ggaggaagct ctaagctgac acaagcggat
     8941 aatgatttca ataatttgca tgataaatta ggagatccga agtttggtca aggtactgtg
     9001 tcctttaaaa tatctgaagc aaatccgacg tttgtgattg gttcttctaa tgttcaaacc
     9061 tggtttactt tatcttctgc gacaatcttt agtgttgttc ctgatcaacc tgaaaaaaca
     9121 ggggttgata aaaatggaaa taacgtgaac gataaaatgt tgcaagtagg agacacgatt
     9181 caatatcgaa tcaaacagaa ggtgaatcga ttaggagtgg atttactagc ggtttacgat
     9241 cgatttgagt taatcgataa tttaccaaaa gaagtaaatt acgtcgatgc acacgttgaa
     9301 agtggtacaa ataaaaaatt cgatgtatct ggtgaagtga cctatgacaa aacaaaacac
     9361 caggtaaaat atgcagcgaa agctgataca ttaaaaaatc gaatgaagta taacggcgaa
     9421 acttacgaat tagtgatcaa tgtaaaagtc aatgaattgg ccaatcaaaa tagtgtagcc
     9481 aaaaatcagg gaacgtcgat cattaataaa gtagaaaagg atacaaatat cattaccgtt
     9541 tatttcccta aaattccagt gaaagaagta caacaaaatg gaaaagatgt aaacggacga
     9601 aatgacggta agaagggtac gccgacagct cctttaaacg caggatctga agtacaatat
     9661 ctagtcacac aaaaatggca cacaaaaggc gtagacgctg cgtcagatca ctacaaacaa
     9721 tttagtattc aagatccaat tgaagctcgt ttgacatata aagaaggttc tgcgcaagta
     9781 atagataaga gtaaaggaaa agatattact tctgaaggca cactaaccta tgatagcaat
     9841 tcaagaacgc tgaagtggga agcttccgct gacttcttaa ataacaatct tttagatgga
     9901 cgagaaattc agctgatatt tacagctaaa actccattac aatcagaaaa aaatattgat
     9961 aaccaagccg tagtagcagt agataatgtt tctaataaaa caaatgttgt gacaattggg
    10021 gttgatccta acttgccaca agttattgtt cctaaaacag gttctacaca tttagtaacg
    10081 attttagcgg tcagcttact attacttgtg ttagcaactt ttggctacgc agttctgaag
    10141 ttcaattaaa caaaaaaaca aggctgaaaa tcagccttgt ttttctattt gaaaatggga
    10201 ttggcactaa taaatacgtt gtctgaagaa agtgatccgt ctgcagaaac ttcataagca
    10261 tgggataaac caacgatact ttgattgttg taaagcaagt ttgcgttatg agcagcgcta
    10321 tctttccaca taaccatagc atttactgct cctgtatctg aaaggatctc gtcaacgtaa
    10381 aggtagaaac tattgatatt ttctccttta gcaccgtagc agcctaagct ttctaagaga
    10441 tcttggaaag taccgccgcc aggtcggtaa tgactgaagc ttacttgtaa ttcttttgca
    10501 cgaatatctg cagcttgttg aacgatatca ttgaattgca ttggggcaac gccattttgt
    10561 tgacggtagc tatttaacca agcaagttct ttttgacgca catagttgtt taaatcactt
    10621 agtgagatat ttttgtattt gaaagtgatc gttcgatttt ggtcaacatt attgattaca
    10681 cgttgtccat ttttatcaac ggtaacagat gaatcgtcat ttctacacaa gaagtagttt
    10741 tgatcggtca tatttttcca agaagtagag aaactatctc cctcgtaaac cgtttgtgtt
    10801 tcagtagata aaacagcgtt tgtgtcaacg tttatatggt tcacagtgac gttgtattga
    10861 tgagtatagg caaaattgat tccttgatta cttgtaacgt tgtttaaagt cacagtgttg
    10921 ctagagcttt ctctaggatc aagaacaaaa ttagtgaaca taggtgcttc aattgtgatg
    10981 ctttgtccat aaacgacggg ataactctta ctgtaattat agagttcttg gatatttgga
    11041 tcagtatccg agactttatt tggacgagct ttcacttcaa ctagaaagac ttcatcatag
    11101 taaaaagtaa atgtggtatt tgtgttaatc gtttgtgttt gtgtttcatt cccatttact
    11161 tgatactgtg gtgcttcaaa gttagtcaag tcggttctag cttttgcagt gtaacttgta
    11221 ccagctttta ctgagacatg ttcttctttt agcagcgttc cagtttgtcg attcatatga
    11281 cggacagtta ccgcatattc tgaaggttta ggtttgtgtt gtaacccttt aaatgcagaa
    11341 tcaagactat ttttagcatt atttacttgg gtttgagaag ctttagaatc gtctagtact
    11401 tttttagcat tggataaagc agtctgaaaa ttattccaag atccttctgt aaaatctcct
    11461 ttagcagttg attttacttg gttgtacaag gcttgtaatt ccgttttgtt cacagtagga
    11521 gctggttttt tctttaatcc cttgtaagca gaatctaatg tgtttttggc actatccact
    11581 tgtttttgag tcgcttttga gtcatttaac acttttttag cattgtttaa agcggtttgg
    11641 aatgcgttcc aggtattgtc ggtatagtca ccttttttag ttcccttaac gctcgtataa
    11701 agagcttcta aagctgattt attcactttg ggtttagagg tgtcagcacc ataccaacca
    11761 ataccttcgt atttccagcc atagtctttg gttaaccatt gtacttcttt ccaacttgca
    11821 gtgtagtgat gcgaaccaga ggtaacacct ggaaggaaaa ggcggtagat cggtttatta
    11881 tttttttcgg cagaacgcca ggcaggccct tcataagtcc aaccgtaatt tttggtcaac
    11941 catttcactt catcccaact agctgtgtaa tggtgatcac gaaggccttt attgtataaa
    12001 cgatagacaa ggtggccacc gctagtcggt gcttcccaag cggggccttc ataattaccc
    12061 cacttaattt tcactaggtg atcacgttct tttgtgctag aggtgtaaaa atgttcatgg
    12121 ttatttggat tataaaggcg atacatgacg ttagttgaag cagcgtcagc agatccagca
    12181 agagcaactg tggcagcaaa tgttccaata gctataacag tttttgggtt taataatttc
    12241 atgttttcaa ctccctatta aatgattaaa tattttgttt acattcataa tataccacgg
    12301 tataccgagg tagtcaacgg tataccgtgg aaattttgaa ataaaaaagc cgccctatga
    12361 agagtgtaag gatggctgtt tatatgctat gaagaaatgg attcggcgta tttggttagt
    12421 ttggcagcat tatcaaatcg cattttttca atcgcacttt ttttctttat taaatccgca
    12481 attgttgatt ctgcgactga agaatgtttg ctaatttcat atgctgtctt gccagtttca
    12541 aatagtaacc attcgatttt ctctttatca gcgtacattg caatgctccc tcttgatttt
    12601 ttctttatta tagctctttt ttgtaataaa tcacaatatc aagaggaaaa gtttctatag
    12661 aaaggtggga aattatgagt atccgttccc cgtgtatcaa aatgaggttt gttgctactt
    12721 tcgttattta atatactaaa ataaaacaac gaaagaagga atagaatgaa aaagttaata
    12781 atggtattta cttcgttgat tgccatgatt gttattttag gtggatgtgg tagtgaaaaa
    12841 aataagcaag atagtcaaga taatggcgtg ttaaaaccag gaacagtgtg gaaagaagaa
    12901 gtaggcggac ttgttcataa tttaaaaatt atagatgata caacatggga atattccgaa
    12961 agtgtttggc atcctgaaca agtacaaata acagttgaga agctaaaaga ctataaagga
    13021 ttagaaagat ataaggtaat cgattcaggt ggagtagatt tattcatagg taaaacagcc
    13081 ttaatagttc cctataacaa agacggttta gaaacgttta aatttattcc aacaaacgaa
    13141 aaagaacatc gagataaaag tgaaatatta aaagaataca gtactaaatc tggatacaag
    13201 ttacaaaaga ctagcgagtg attgctagtc tttttattta tcctgaagga ggaatcgcag
    13261 tggcaaaagc taaaacgcca tttgaaaaag ccgcgtctaa aatcttagaa cgtcgcggtg
    13321 tatctgaaac acagtggaaa caagaagtcc ttgaaaaagc acaatttgac ctattacggg
    13381 gaaaagacaa ggacttagaa ggatatatct atcaacgtga acgtgaaaag cttgttttcg
    13441 atgaaattat taaacactaa aaggagcgaa caattttatg tttgaatcaa ttacaatgaa
    13501 ttttttaaca caaccgaaag tagtagctgc ggttgatatt tttagagcat tacaaggtgc
    13561 agcaactagt ttaaaaaaca atctgatgat ttttgcggct gtagctgccg ttgcctccat
    13621 tgttcttggg gggattttct tcttctttgg ccgaagagga gctgaaaacg ggaagatgca
    13681 cgtaggttat gtattgatgg gggtaggagt tatattagct gccgcctcta ttgtggtttc
    13741 gttatttgga atgtttggac aaaccccaca gggactataa aaaataaata gaaggagtaa
    13801 aaagtacgtg gctacttttt actccttttt tatgaaagga gaatactcat gtactttacg
    13861 atttcaggca ataccaaaaa agataaagag ttaataaaaa aagcaacgaa gaaagtggga
    13921 aagcgtactg cctttttacc gaaaacaatt aaatattctg tcattgaaac ggaagaacag
    13981 ctacatgtgt taggacaagt gttagaacaa gagaatcaat cttctgttaa attgactctc
    14041 acgttacatg ataaagaagg aaaaatagaa aaggctggca cagaatacgt ttttggccca
    14101 ctggattatc aaatttcaga aggtttttat cctacattga tggatttgat ccgccttgat
    14161 gtgtttaagg aaacggatga aaccatgagt gtggaagagt cttatcgaaa cgaaaaactg
    14221 ctacaagttt tagaagaaga gttagagagc gaccaaagaa aagtgagtca agtgagtgaa
    14281 gtcgaagtaa atgatgaagt accatgggaa cgaccaatct ttccaaagaa ggagtcaaaa
    14341 gaaaaagaga cagtctatgt tgacgaacaa gctgatattc cgactcagaa atcgatattt
    14401 gaggaagaga acgatgtaat gggtcaattg gaggtagatc cctttgaaga aacaatggag
    14461 gatgaagaga cggccgaagt agaagttgat ctagttgaag ctaatgaaga gccaacatgg
    14521 actgaagggg attctcagga atatattgaa ccacttatca gtgaagaagt agagaaaaac
    14581 gaaacagaga tacaaaaggc aactgtattt gattatattt tggatcaata tccgccagag
    14641 catgaatgga tgaaagaaaa aaataaatta tttttaaatg agctttattc gaaatatcaa
    14701 ttaccccaaa cctatcaagc atttttagag agtaaagaag aattaatctc acagggtaaa
    14761 ggcacacttt tagaacagtt agagtctgtt caatcaacaa attggcatga gcttgcacaa
    14821 gaagatttat tagccacttt tgaagaacgt caacaagaaa cagatcaaga aataaatgag
    14881 tatgcgacgg aacagtcatc taattgggag agaacaaaag aggaactaaa taaagaagaa
    14941 caacgagtga ttgaagaaga agtacaaaaa attaaagagc gatacgaaaa gaaacgcgac
    15001 aatgcgtatt ccttgtgtat agagaaaatt aataacttta ctaaaactaa tcaagaagct
    15061 ttatcgaaag aaaaagaaaa cttgttaaaa gaacgtataa aagaacgaaa agaagagttc
    15121 taccgaggtt tacgtacaga taagctaaag attagcaaag aattaaatga acaactgaat
    15181 aaactttatc aaacaacgac tgaagttctt atggaaagac acgggcagat ccagcaagaa
    15241 ttacaagcac aaattccaaa atggaaaaaa gagtatgaaa ttcaacaaca agaattgaaa
    15301 gaacaagaag aaaaacaacg acaagaagaa aaagagcgcg aacaattaga actaaaaaaa
    15361 cgtgagcagc atcttgcaga acaaaaagtg gcgatagaaa aacaacggtt agagcaagaa
    15421 aaggaaaaag aagcagctgc tcgtaaggaa cgtcaagaac aaatggatac attgcgacaa
    15481 gcacagttga attatctagc acaacctcaa ccagtagctg ctgttcaaac gccagtgaac
    15541 aaccaagcaa atttaactcc ttcaccacag cgttccttta acggatggat ggtgggatgt
    15601 attgcaagtt taagcttgct tctaggtgga ggaacagtat ttgcttttaa tcatatcaat
    15661 caatcttcac aggcagattc tgtagccaca acggcgtcaa tcaaggaaga agcccaggaa
    15721 caagcaagaa aagagtatga acaacaattg gaagaattat tgaatcgtac caatcagtca
    15781 acagcagagt caagtatgga aaatagcaaa gagagtgatc ctagtaaggc taaagaagga
    15841 tcaagcgatt caactgtaca ctcaaacact tccgaaagtt cagaatcaaa tgaggtgaaa
    15901 taatgcgtat tagagaaaca acgagacgaa aagacaaacg attcaattta gaaaagaata
    15961 ggtatctttc tcatttctat cgctatactc cttcaggaga agagctacca gaaaaacatg
    16021 acaaacgtat ttctcaaaag attttttata gtggcttagc ttatcttcct ttaaactatg
    16081 ttgttacaag aataccagaa gcatttcatc gatttgagat tattccagga agtttacaag
    16141 gagtgaatat ttttcagtta caagaaatgc cgcagttatt ccattttgat tcaactggtt
    16201 tttggaatcc tttgacccca acaacaagtt taggcacacg tttagttgtt tctacgattt
    16261 gtgttgttcc agctgtactt atgggattga aaatggacat gtggtttcgt ccgctagcag
    16321 atgggcaaaa aggagataac cgattaaaaa cgttaaagga aatccaacaa atttatccgc
    16381 aaataccaga atcaaaggga agttttcctg gttatggcgg aataccaatt actcattata
    16441 aaaaatactg gtatattcag acggatacag taaatacttg tattgttggt acttcccgct
    16501 cagggaaagg ccagatagaa gttttagcaa ccattgacaa tttgtcgcgt gcagaaagac
    16561 aaagttcaat ggttgttaat gatccaaaaa cagaattata tgttgctagt aaagatgaac
    16621 tagaagctag agggtatgat gtgtatgcct ttaatatcac cgatccactg caatcaatga
    16681 gtgataatcc attggcactc attgtgaaat actggaagcg aggagacata gacaccgcaa
    16741 cacagttaac caatactttt acgaatacga tttattatga tgcaaatgct tcagacaata
    16801 aatactttaa cgaaaatgcg cagaaagctg tcaatgcgct tatttttgcg ttattggaac
    16861 aagcggatca aacaggggat tactccaaat taacaccaaa taatctcgtt gaactacttt
    16921 cagaaattgg cggttttaat tattatccag atcctgagaa tgaattcaag cagctcaatg
    16981 ccttagatga gttcatgaat cagttacccc ctggaaatat agcaaaaaaa caatatgggg
    17041 caacaaaaat tggatctgat aaagcgaaag ggaatatctt gtctacggca attacaggat
    17101 tgaatccatt taccctaacg aaaattgcga agatgacttc tcaatcgacc attgattaca
    17161 agtccattgg ttttccgaag tatttggatc tcaaattaca tgaaagccta ttaaatcaac
    17221 gcattgtcgt ggaattttat cataagggaa agaaatttca cgaggaacga gtcaaagttg
    17281 gctatcgtgg tttttgtgaa atcaactttg attgtcattt agagcaggga gatcatgttt
    17341 gcttaaaata caatcatcaa ggaaaacaat atgttgcttg gtatgagttt tctcaaaaag
    17401 tattgaaaga cgaagaggga aaaatcgttt atcgaaaaaa acgaggagaa actcatttgc
    17461 cagaactaga aaaagaagcc gttctaacat tagatgagaa gaaaagtaac ctattagtag
    17521 agtctattcg gatgcactat accgataaac caatggctat ttttatgtta acgcctgatt
    17581 atgatccatc taaccatatc attgttagca tctttcttag tcagttgtat aaagaattat
    17641 ctacgcaatg tgttaagact aaaggagata aatgtcaccg taggattcat tttattttag
    17701 atgaatttgg aaatatgcca ccgatggaca acatggaagg aatcatgacg gttacggcag
    17761 gtcgtaatat gttatgggat ctgtttattc aatcttatca gcaactttat gcgaagtatg
    17821 gagaacaaaa cgcagcgatt atcaaagaaa attgtcagac acatatttat attatgtcga
    17881 cagatgataa taccatcgaa gaattttcta aaaaagtggg gaataagacg gttgaacaag
    17941 agaacagtac catcaattca ttaggtatga atactcatat caatcgcaat gttgattcag
    18001 atcgtatctt aactcctgaa agaatttcta cttttttaga aggtgaaacg attgtactcg
    18061 ctcctttaaa acgacaagat ttgaaaggtc ataaaatacg gccgtatcct atttttaatc
    18121 atggggaaac gaatcttcct tatgcttatc aatatattga agatttcaat ccatcgtggg
    18181 atatcaatga tatagacata cattgtacac atgcggactt ggatcttaag gctttacaaa
    18241 ttgattatga ttatttccta ccagatgaat atgctagaaa tctctatcat ggactgcatg
    18301 atcaattaga agaaaaaaac gaagaaaata tgttttataa aatcgttcaa tcttttgaga
    18361 atgagacggc taaaaaattg ttaattcaat tttacgaaga acaagcaacg gatcgtcttc
    18421 aagaatatgt tgaaaagttg atcaaaaatg aagaaatttc tgaaatgatt aggaaacggc
    18481 tgttattgga agctgaaaag gcggtgaaag aatgatcatt ggaggaattt tctctggcat
    18541 agccgactgg tggtcaggtt tattcggaga aggggatgca gatcccaaaa ccgtaacgaa
    18601 agtcttacaa gattatggta attatctgca gtatcaagat gtattcagtt ggatttgtaa
    18661 tactttttta tggggattga tcaaagggtt atataatctc aatactttat tagaaaaaac
    18721 aatttatcaa agctttgatt taaaagactt attgaacgcc gctggtgtta atgaactatt
    18781 tcaaagtttg attagtaagg tattggctct tttttgtgtc gtcacattaa tttatttagg
    18841 cttgaaattt tttatgagta aacacccacc gaaagtcaaa aatatcttgg ttaatttatt
    18901 tatggccatg atcttgattt ttggtggtag ttctttaatc gatgaagggt taaatatcag
    18961 taaaaacttt tatggagacg tcactgcagc aaataaagca gataaaacgg gttcacctgc
    19021 ttttcaaatt attcaaaata atgtgattga tgtcagtaca ttactagcga ctgacccgaa
    19081 taaagtagaa tccttaccaa cagataaaag aaatgcttta acagcgaaaa atttcaaaac
    19141 agcgaatatc aatgggatta ttacacctga tcaagctaag aaaatgtctg aatcaagtga
    19201 tattagtaca gtacaaaaag atcggttaaa ggcattacag tatcgactag agttagacga
    19261 taatgggaaa gaaatccctg ttcaaattag tgatgaaggt ttagcaaaat atgtgtatac
    19321 gtcaggctat cgtcgataca ttagtagtcc agggattatt ttggctgggg aatcttcttt
    19381 gtcggtagcc tatttattta ttttgtttac aatcgttact tgcattattg agctagtatt
    19441 taaaaaattc tatttagtaa ttgccgcgtc aacggacttt gagacaggac aacggatgaa
    19501 aagtgcgata gaagatattt ctcaatcctt cttgttattg gcgtttacga ttttagaatt
    19561 acgaatctat acattaatgc tttcaggtat cggtgatttg catgcggtag gaaagatcaa
    19621 tggattcttg tatgttgttg ctttaattgt gctaactatt gcgctattca aaggctctca
    19681 atcggttacg aagattttcg gtgttgatac gagtctgaaa aacggcagca attctcttct
    19741 tagcatgttt gccttaggaa atattgcgaa aaatgtgggt agcggagcta aaaaccttgc
    19801 aagtgctggt aagagtggtt taagccattt aaatgatatt cgtaagaatg gatttggcca
    19861 tcgaaaagat attacccatg ataacgggga agaacaatca gacgataaag ctacaactgt
    19921 acctaatagt aagaagacag tttccgaaat gttcgggaaa gcgaaaaatg gtgtttctaa
    19981 agccgcagaa ggtcttggtt acgtcaatga acgtgggtta aaaggaacag ttgaagatct
    20041 tacagaaaaa gccaaagaca ccttagaaga gaaattagac ggcacgagag ctaaagaatt
    20101 tgctgaagct gtgaagaatc catccgaagc actagaaaaa caaaaagaaa aagcttcagc
    20161 agtcaaagaa ggactaaaag atgcgtatgg tgaaggacaa gtcaatgcag cagtgaaggc
    20221 aaatacaaca agtgaaaaag aaaatcaaga aggtgcagcg attccttcag ccaaagatca
    20281 ccaagatgag ttacattcta acgaaaattc gatcggagaa aaagattcag aagaaggaca
    20341 acatggtcag ttaccaaaaa ccgatccaga acaatatcgt tataaatcta acattaattc
    20401 tgaaaacgaa aaacgcattt cagatttacc aaaacgaaat caagaagtac aaacggatga
    20461 ggattccatc gatctgccaa atgataatgg catcgaagtt ggaactgaaa aatcaacgaa
    20521 aattggtttg cctaagacac aaaatttacc aatgaaagat agtcgtttga atcaattcaa
    20581 agagaatgat caaagctttg ttcatcaatc acctgcaggt actgttcaaa tgaaatcaag
    20641 tcgtttgcct gaaaatggaa atctgacaac tgtttcttac aatttagctg aaagtggtga
    20701 aacagagaat gcgtccgttc aaaatattgt gacaaaagaa gctgcaccta agccaatcaa
    20761 taaagttcat aatggataca gaccagcaaa cgtagcattt actgcaccga caaaaataaa
    20821 tgtgacaaga gaaactaaaa acaggaacaa tgaattgaaa gaacaactat tgaaggatat
    20881 caaaccataa aaggcctatt taagggcctt tttctttata aaacaagttt aaaatggaaa
    20941 ggagaaaatt gtggaggata ttttaatagc tacgtttata actgcactgg gagcattttt
    21001 aacgatattt gtggtgacta ttaaaggatt taaagatacg gatagtttac aaaaagatca
    21061 tcagtctatt ttagatggtc aaaaaacaca taccgaaagc attattgtac acctttcaga
    21121 aggattcact tatttgaaag atgctatgcc cacattcaca gtaaaattaa cggaaatatt
    21181 tcattctgtt gagcaaatat cgtcagaatt gaaagagcaa agacaacaaa aagatcgtca
    21241 acttggctca cttaatttta ggcaagaaac gatacaagaa tcattggaga atgtaaatcg
    21301 attaaacgat gaaatgcaac gagttcattt ggaaaatgaa gaattacgcg cacaaaatta
    21361 tgatttaatt caaaagaaaa gaaatctgga acaaaaaatt gttgaattaa aagaagaaaa
    21421 tcgtcaattg aattatcaat tacgacgagg aaaaaatcgt tctcgtgatt taggaaatga
    21481 attagagttg taatatttag aaaaacgctg tgagaaatca cagtgttttt tatttctaaa
    21541 aatacaaaat ataaggggta tggttatatt atggataaat acattcatat gaaaaatcct
    21601 ccaagattta gtggggaatg gaagtttttc gcctttttct ttttagatct attaacgctt
    21661 ggtggctgct ttttttgggc gaaaatagtc aataatcttg tccacttacc actctttttt
    21721 gcactcttga attatggatt atggcttgcg atagcgttag tgattgtttg gcgaccctat
    21781 aataaccctg gtcaacgcca aatttttgcg attatcgaat ctattttctt tacggataag
    21841 aaccactatc atgcgttaga tcctaaccga aattatcatg gtacgtataa ggaggatgaa
    21901 taaatgcgag tcaaaggaca aaattcagtt gataagaaat ccattttgca gtttggaaaa
    21961 ccagaagagc gctctatctt ggacttattt ccatataaat cctgtacaga agagggagtc
    22021 ttaattacta gagaagatca ttttcagcgt tattatcgtg tcgtttccac tgacgtggag
    22081 ggattaaatg aacaagagaa actagaacga atgaatcagt tgaccaccgt catgcgtacg
    22141 tatgtgccaa caattaagtt agttagtttg accacagaga cggatctaac ggaacaaatc
    22201 gtgcaaaagc ggcagttatt agataaaaat cgtatggaac agtcactagg aagaaatcta
    22261 cagcgattaa gaaaatatga aaagaaatta gtagaggaga tccaggaatt aaaacgggca
    22321 gaacaagaac gtcctgactt aagcttcttt ttcgttattg aaggaaagac aatcaaggat
    22381 ttacaaacta gaaatcgtca gttaatgcga tctagtggca ttttagggtt aaaaccactc
    22441 tacaaaaaag aattgattaa gattctttat cgaatgaaca acatgaatga cgaataaaaa
    22501 agatggagga aatgattcat gctagatgtg ataaaaacag ctttttcaac gcaaagtaat
    22561 caaaaggtga ttgatccatt aaaacaactt ggtaatcgtg aaatagagat tttaaaatca
    22621 aaaggttatg atttagattt cttaaaacaa gtgatgccac aaggtgggtt gaattttgat
    22681 gaagaatatg tgacctatgg aaatggttat tcacgatgtg tgacagttta tcattacaca
    22741 aaaaatccta ctcttttttg gttggccaat ctcatgaaca atcctcatac agtggccaca
    22801 ctagatgtta gcacagatga aaagcaaaaa gtgattgatc aaatcaaccg cgcattagat
    22861 gaattaaatg atcgatcaga taatgaacga aaagcgactg accgaaataa aagtgcggaa
    22921 gaatacctgg aactagcgga atatgcccga aaattgactt ctaatgggga aatttctaaa
    22981 caagtcaaag tgagagtaca tgtttacgga aatacgatcc aagaagtgga tgaacgggcg
    23041 caggaactga tcgaaaatct aaaagggatg gattatcgtg cggtgactta tctgcataaa
    23101 accaaacgag attggcaatc gatgtttacc atgttcgatg aacaagctga atggtttgga
    23161 aatatcaaag ggcaatcact tccatcgtta aacattggcg gaggattccc gttccaccac
    23221 caaacgctaa aagatccagg aggcacacaa tatggggaaa ccatgacagg cggcccattt
    23281 gtctggaatg ccacgtataa aaatagtgta cgtttatcta ccaatacctt gcttctagga
    23341 atgatgggag ctggtaaatc gaccgttctt aaaatgatcg cggaagccca tttagctgca
    23401 ggagacttta tttggggctt tgaaaaggga aaagacttta ttcctttctt aaaggaatat
    23461 aacggtatta tggtgcgttt agacggatct gacgggatga tcaatccttt agagatcttt
    23521 gccacacgga cgtatgatga agcatccagt ttatatgacg atggttcagt caaagatttg
    23581 aagatcaatg aagcagcgtc ctatcaaacg cacttagaca aagtagtcta tcaggtacag
    23641 ttagtgagtc ctcagctaaa aggaacgatg aaagcagaat tcaagaccta cttgaatcgt
    23701 ttttatgaag agtatggaac agtaccacgc ggttttactt cctcaaattc gcgttcaaat
    23761 actgatacgc aagtcacagg gaaagatcca gaagcttatc cgacgttcaa agaattttta
    23821 gcctttttag gacaactgga actaccaggg gcttcccaag agaaaaagaa tcggaaagaa
    23881 gaaatggagt ctatcgtgga atccttatgt gagacttatg gcatgatttt tgatggtcat
    23941 agtacgattc gacaccttga ccaacaacag ttggtctttt ttgacattga ctctattaca
    24001 ggcctagatc gaggggtaca acaatgtcag atgtacatgg ccatgacgtt gatttggaat
    24061 caggcattga ttcaaggacg aagacaacga cgcttaatta aagaaaaaca aatggaagaa
    24121 caagacttca aacgcttctt ggcgatgatt gatgagtgcc ataatgcgat taatccagat
    24181 ttcattgaaa cggtaaatta tgtcacaaac ttccaacgag aaatgcgtaa agtagaagca
    24241 atgactgttt tagctacaca atccccacaa gaaatggtgc cagaaaatat gtcttctgat
    24301 aacttctcga aactgaagaa agtctttgaa ttttcttcta cgaagatctt tatgcgtatg
    24361 gatgaatcta ttttacgtca tatagaagga ttagttggta aatcaatgac gaactcagaa
    24421 ttagaatcca ttcctcaaca aaatcaagga gacgctgtga tcaattttgg ctcaaaagaa
    24481 accattcgtg ttcattttag tccgaacaaa cgtcaattaa aattgtttga tggaggaaaa
    24541 taatggaaaa ccaaaatgaa agtttaatca aacagtatgt taagcgtcgt gcaaagcggc
    24601 gcttattttt gtgggtgttt ggtacttctg caggtttgat tactatattg atcactgtgt
    24661 ttgtgaccct ttttctaata cttgcggcag gttctattga taattctgat tcaagtagtg
    24721 gtggcgaagc ttttacagga gaatattcgg aaggcttacc catttataaa gaaatcaaag
    24781 gaagaggccc tttttctgat gagattgcgc aatatgctgt aggtgcagca gtgaagtata
    24841 aattattacc aagtgtgatc ctttcgcaat acggttatga atcagcgttt ggtacgtcgg
    24901 cgtccgcaag aaatgatctc aattattttg ggattacgtg gtttgatggg tgtctgtttc
    24961 ctaaaggaac tgcgcgtgga attggtggca ttgaaggtgg ctggtacatg aagtttccca
    25021 actcaaaagc tgccttctcc tattacggct ttatggtagc cacacaatca aattttaatg
    25081 cttgtgtcgg aaataaaagc cctggcgcaa gcttattgat cttaggacga ggagggtatg
    25141 ccgccgcagg aattactgaa gacagtgcct attataaaaa ttgtatgtct attattaata
    25201 caaataaatt gatggagtac gatgagtttg cgattaaaca ctggagagaa ggcggcggaa
    25261 ctggtggtac gattacagga agctggggca atccattccc aggaagtagc ttagataaaa
    25321 attcgttcag tggaggacaa ctttttggga aaaatccagg aggagaattc cgacctaacg
    25381 ggttccatga tggactagac tttggttcag tcgatcatcc aggaagtgag attcatgcgg
    25441 ttcatggcgg aaaagtcgtg tacgttggca atcctggtat ttcaggtttg ggtgcgtgtg
    25501 tgatcgtgat taattacgat ggcttgaata tggtgtatca agaatttgca aatagtacag
    25561 gaaattctcg agtgaaagta ggcgatcaag tgaaagttgg tcaagtaatt gggatacgag
    25621 atacagcaca cttgcattta ggatttactc gaatggattg gcgtcaagca caaggacatg
    25681 cattcattga tgatggtaca tggatcgatc cgttaccatt tttgaatagc agcaaaaaat
    25741 agatcaggag aaaaaatatg gaaaataaaa ccttaaaaat cgtcttaaca gctgcaatta
    25801 gcgttagttt aatcggcaat attgcgttgt tttggcagct taaagaagaa caagagacaa
    25861 atacctataa aattgaagaa gtggctaaaa acgcgtctga taagctcaaa aaaaattctt
    25921 ctgaagcaca ggcgaagatt gacaagttaa atcaaaccat caatgagaaa aaccaagaaa
    25981 tcgatcagct gaagcaacaa gatggcacaa aagcaaaaga ggaagtaact gaagcacaaa
    26041 gaaagacggc agaagagttc ggtcaattgg ccattgataa atcattaagc agaaatgaat
    26101 taacggaaaa actaaaagat gtcgcaaccc aagaagtgat cgacaaatta agtcctgtag
    26161 acgatgatca ccaacatgat gattacggaa gttactcttt tacattaggt gacgtgcaaa
    26221 cttacgctca aacaagcagt gtaaaagagg aacaaaattt tgtcgtattt gttgattata
    26281 caatcggcaa tcctcaattc aaagatgtca aaccacaaaa aataaaaggt ggattaactg
    26341 tcactgaaaa acaagtagat ggtcaatgga aagtcacaga ctttagttat ttcactcgtt
    26401 aggaggaaga aaatgagtaa gaaaaaagga gcaagacaac gttttaaaaa acgaaaaagt
    26461 tatcttagat ggctggggat ctttatgatt ctcggctttt tgattcttgc tagtccgatt
    26521 tacacacata aaaaggtaga cataccagat gaagtaagca cgaacgcatt tttaaacacg
    26581 aaggaactga cgatggtttc aaaagaaaaa ggagcgaacc agcaattagt ttttgaattt
    26641 gcgatcgacg atgaagatca gagcttatta aaagaactag ctaatcttaa ttatcgtgta
    26701 gaagtaaaga cagctaaagg ggattatcaa gcaataaaag ccaaagtgat caaggtgtct
    26761 gatgattatt ttgtagtgaa gttagcacat gtaccagaag aatatattgc tatgcgatta
    26821 actattattc ctgaaaaaat tgatccaaag gtggatatgc aagaaccgca agatctgatt
    26881 ttttatattc acgaagataa ggtcaaagat cacgtgaaag acgaagatta tgaacaacat
    26941 gcgatcaatt acaaagtgaa aggatacaag aaagaacaaa aagcagcaca aaaacaaatt
    27001 gaatccttcc aagcaaccat tgatttgaat gaagaattag tgaagaaact aaaagatcaa
    27061 ttgccttatc aagtcgcaga agatcaggaa aatactgaaa ataagattaa tagttaccag
    27121 caagaaattg aaacatcgaa aacgcaaatg aaagatcaag aagagagcat tcaaaaactt
    27181 caagaaaaaa ttgatcgtat gacaaaagaa aatatttaga agaataggtg gagtaaaaga
    27241 aatgaagcga gcgaacttgg tcggcaaaga tactttcgct gagcagaaag tattgattaa
    27301 agcctatctc aaagttgtaa aagctaaaga aatatgtcaa gaagtgaatt atctaacaca
    27361 acaatatctg agttgaagaa tggtagaaag agtttagata ccttaaaatt cagatcattt
    27421 gaagctctat atatagctgc taaaaaatat tttacagctg ataataaata aaaaattaag
    27481 gtgggttcta tgtgaaaaaa acgtatcaaa agtttttatg gattagctat atcgtcctat
    27541 ttatcgttgg gtttctcgtc agtgtgtccg tgatcgtcgg aacgcttttt cttttgttct
    27601 ctgacgaaga tctggtactt aaggggttaa tgagtacgtt atcgtgtgtc atgttacttt
    27661 atttatgtat acagatcaac gaaattatgc cccctcattg gcaagaaaag aagaaagaat
    27721 ttaaggaaga aatcgaaata aaatccaatg actggattga atacacatgg cttcctctga
    27781 aaagaaatag caaacgaaag gaattttggg cattgcaatt atggccaata ggaaaacttt
    27841 ttatttatat ggcttcactt atggttactt taatcggtta tttggtggtg gtctatgggg
    27901 tggcctatta cttgttaagt gagccaaaac agatcgcggc gtcaaatatt ttcacggcag
    27961 taatgacatt aatgtgtgtc atgtgctttt ctatttttag ttacttactc gtccaattta
    28021 tcaggcaaaa tttaacaatg agtaaacttt tcaaaagttt attggactta cttatcttag
    28081 gcagtatgtt aacgactcta acagatcggt tcgaacaatc tgaaaaaacg gtagagggga
    28141 taaagaactt cttaacgacg attgatcaat ttataaatgg atatatgtat ttgtttgaag
    28201 taggctttat tctcactttg tttggttgtg tatttataaa actcattaaa agatcaatcg
    28261 aaaaagaaat ggttattgat ctaattccga cgctgagcgg ctatgagtat tacgttgatt
    28321 ttggaaagta tgaggagttg atccatacgg atatcgttca cacgagtatc catgaagtaa
    28381 ccgttaaggg catcagatct actttaaaag atggacagga gacgaaaaca atcgaccgaa
    28441 gaaaagaggt ctacaatttt tataaagttg aagcaagtct acgatatggc attgggaaat
    28501 tgaggttaga tgggagtaat attcaacaaa acaaaccata tgttttagga aaagtggtga
    28561 tttagaactt cctatgaaag atgaataaat aaggagaatc ttttgaaaat gattcttctt
    28621 tacagaattg tgcttatagt tggcacggag ttaaaaatct taaagaagga gcagttattc
    28681 aaagtgaaag aacatcataa tcagttacta tgtattaaaa cgggtacgat gaacattcaa
    28741 taccgaacag aatatttaga attaacgaat caaactgatt ttttgtcgat tcgagagcaa
    28801 cataagcaag aaaaattaat ggctacttat agccaattga ttgatgagtg ggaacaattc
    28861 aaggaatcta aaacataaaa ttccatttaa acaccttatt ttttaaagag cataaggaaa
    28921 cgtgtaccca ctttcaaaat aaagtgggta gataggctgt gcctatcacg tttctgaaaa
    28981 aaataaatga ccccaaaatt ttcaatttat gtgtaggttt atgggtagca aaaatatgaa
    29041 tgtgggcagc aaaaagttca tttgtgggta gtaaaattca aatgtgtgta gcaatgtgtg
    29101 tagctacaca cattgctaca cacgctataa catatagtga aaaggagata tttatggaag
    29161 taaaaataag agatattgat gaaaaaacga ttcaaagaat tgaccgaaga tgtattgaat
    29221 taagtgaacg caccgggact aaatggagcc gaaatcgata cctaaaagtt ttgattgaaa
    29281 atgattttga ccatgaactt atggatcata gaaaagatca atttgatcgg ttgttagaaa
    29341 aatttgtgga tattcaaact tataatacga aaattttaga agaatatatt gcaacaaata
    29401 atcaattaat cgccttgtta attgaataat aaaaatagta ttgtgaattt ataaaaggag
    29461 gaaatcaatt ttggcaaaag caacaagaaa acatgtatat ctttacgata tgcatatcga
    29521 agtacttgat gaaataattt ctactcatga agatatatct aattatagtg aagcagtcag
    29581 acatttgtgt atgatgtatg atccaaaaat cgaagaaaaa ataaatccaa ttcaaattaa
    29641 attgaatgca atgggaaaag aaatttctat attaaccgaa atggtttcag agtttgcgga
    29701 tcttcatttg aaagatacag gaatactaat tggtacaaat tcaactgtct atcaagaagc
    29761 aaaagagcgt gtggaaggga aaataaaagc gaatcaaacg aaaaagtatt ctcctaagat
    29821 caagaaacca ataaatgaat ctgttcatgc cagagtagac ccgtttgatc caacaaagtt
    29881 tttaaaaaga taaaaagtag aaggggaaca tgatgaataa atcgcctgca gtcattctca
    29941 cttgtcaatt taccttgccc aataaaaaat ctttttcaac atacattgat tatatgacaa
    30001 gagaaaaagc gctggaagaa aaggaacgac gcacgccgga cgaagaacag gaactgcaac
    30061 gaatcaagaa tgctttagag gatttttata ttcctgaagg agaaactttt tcggagagta
    30121 agacagataa agaattcagt gaaacgtcga atgaagctca atcgttgatg gaacatggaa
    30181 tcaattttga caagttagag gaacaagatt ttaccaaata tctttcttat atgacccgtc
    30241 actatgcgtt agaacaaaag aaagaactga cgcaaaggga acaaaaagaa taccaacggt
    30301 tgaaccaagc aatgaaaaaa tatcaaacag agccacataa aaaagcttca ggaaatcttc
    30361 ctggagtttt ttctatggct tcagacgaag tgaaaggaag tgatctgaag gagatcaaac
    30421 aaatcttcca aagagggcaa caaaaaggct caatcattta tcaagatgtt gtctctcacg
    30481 acaattcgta tcttgaaaaa atgggcttgt acgatcccaa aacagatacc ttagatgaag
    30541 aaggattaaa agcagctgga agaaaaatga tggagacatt attcgagaaa gaacaattga
    30601 atgaaacagg ttactggatg gcaacgattc accgtaacac taagcatatc catattcatt
    30661 ttgcagttgt tgaaaaagaa aatacgcgta aaccttacac ggaggttgaa aatggtgtga
    30721 gttatatcgt accgaaaggt aagagaagac aagcaacatt agacgcaatg aagaccaatt
    30781 atgtacatga gttagaacgt ttttcttatg aaaaggatcg aatgattggt atggagaaac
    30841 gaaatttatt gacgcggaaa agtgatttgc gtaacacctt aacaaaagtg gtaaaagagc
    30901 cagcgcgtta cgatcgacac gcaatgaact tattaaatga agtatataaa accctacctg
    30961 acaaacgttc ggaatggcat tatggggatg aaaaacgaac caaactttca ccatacacaa
    31021 gagagaaact cgatgagtta accaaatatg tgttaacaaa agaacctgaa tatcaagaat
    31081 atatacagat ttcaaaatca ttgaaagaag aagcaaaaag tctttatggg gaaagcaaga
    31141 gagaatcaaa ggatgcagaa aaaaatgctc tctttgattt gaagaagcgc acaggaaatg
    31201 ccattctaac agaactgaag aaacaggata gtcaaataaa acctttaata gaaagtatgg
    31261 atcagatgca atctatttac aatgaacgat cctttcgaac gcttaaacag ttaccaacga
    31321 atgaatacaa atatagttac gaagaattca tgaagcgcag gaaacaattc caacaaagag
    31381 ttgtcacgca acggagtttg cgtcaattaa atcgaacgat taatgcccat gaagaaaaat
    31441 accacgcaag taaagcctac gaagagcaac aacgaagaat tcaaatggaa caagaaagac
    31501 agtaaagaca gtaggaggaa tagcaatgaa acaatattta gatcaatgga aagttataga
    31561 aggatcatta agagaagaac gaatcgaaca acttcccgat tgtttagaaa aagagcattt
    31621 gtttcagata agagaaatgt tgagaaacga acagtttgat ccaaatcaat ttttagtcgt
    31681 tgagtactca gctactggtg tgtactgttg taatcatgtg aagggtgaaa aatattttat
    31741 cattcaagaa tatgagggca agttagcgcc ttattatact acttgggaaa tgaatgaaga
    31801 ggggatcaat aacttcccgt gtaaaagcat tgaagagtct atttcactca cagaatgtta
    31861 gttgttaagt gaataaaaga agtaaaagag tcctgaacgt aattgtccag gactcttttt
    31921 ttcaatgaat tgttttccgc caattcaatg agtggtgcga tttgcgacta cattatatca
    31981 aaagaaagag aagggaggaa gaagaaatgg cttattcaac gattggagtt gttgttgaaa
    32041 aaagcagaga caatctcgtt tttgtgacag aaattcaaac gggcagagct tttgtcgtca
    32101 cagacaaagc ggcaaaagcg tatcaaagtg gcgatatttt aacgttaaat ctgactacga
    32161 agacgttcgt cgatgcggca gaagattatc catt
//
LOCUS       DACEPD010000021        32008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.021, whole genome shotgun sequence.
ACCESSION   DACEPD010000021 DACEPD010000000
VERSION     DACEPD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.021"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            370..690
                     /locus_tag="IW797_000989"
     CDS             370..690
                     /locus_tag="IW797_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1223778.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            844..1749
                     /locus_tag="IW797_000990"
     CDS             844..1749
                     /locus_tag="IW797_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1223779.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1746..2519
                     /locus_tag="IW797_000991"
     CDS             1746..2519
                     /locus_tag="IW797_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1223780.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2700..4124)
                     /locus_tag="IW797_000992"
     CDS             complement(2700..4124)
                     /locus_tag="IW797_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1223781.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4335..4781
                     /gene="nrdI"
                     /locus_tag="IW797_000993"
     CDS             4335..4781
                     /gene="nrdI"
                     /locus_tag="IW797_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1223782.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4846..6828)
                     /locus_tag="IW797_000994"
     CDS             complement(4846..6828)
                     /locus_tag="IW797_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1223783.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6842..7738)
                     /gene="murQ"
                     /locus_tag="IW797_000995"
     CDS             complement(6842..7738)
                     /gene="murQ"
                     /locus_tag="IW797_000995"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1223784.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7741..8802)
                     /locus_tag="IW797_000996"
     CDS             complement(7741..8802)
                     /locus_tag="IW797_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1223785.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(9013..9540)
                     /locus_tag="IW797_000997"
     CDS             complement(9013..9540)
                     /locus_tag="IW797_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1223786.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9607..11109)
                     /locus_tag="IW797_000998"
     CDS             complement(9607..11109)
                     /locus_tag="IW797_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1223787.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11367..12488)
                     /locus_tag="IW797_000999"
     CDS             complement(11367..12488)
                     /locus_tag="IW797_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1223788.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12614..13555
                     /locus_tag="IW797_001000"
     CDS             12614..13555
                     /locus_tag="IW797_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1223789.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13705..14601)
                     /locus_tag="IW797_001001"
     CDS             complement(13705..14601)
                     /locus_tag="IW797_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1223790.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14870..16210
                     /locus_tag="IW797_001002"
     CDS             14870..16210
                     /locus_tag="IW797_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1223791.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16428..16871
                     /locus_tag="IW797_001003"
     CDS             16428..16871
                     /locus_tag="IW797_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1223792.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17375..18793)
                     /gene="nhaC"
                     /locus_tag="IW797_001004"
     CDS             complement(17375..18793)
                     /gene="nhaC"
                     /locus_tag="IW797_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1223793.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19196..20419)
                     /locus_tag="IW797_001005"
     CDS             complement(19196..20419)
                     /locus_tag="IW797_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ1223794.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20450..21289)
                     /locus_tag="IW797_001006"
     CDS             complement(20450..21289)
                     /locus_tag="IW797_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ1223795.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21306..21986)
                     /locus_tag="IW797_001007"
     CDS             complement(21306..21986)
                     /locus_tag="IW797_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1223796.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21988..23061)
                     /locus_tag="IW797_001008"
     CDS             complement(21988..23061)
                     /locus_tag="IW797_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1223797.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(23708..24379)
                     /locus_tag="IW797_001009"
     CDS             complement(23708..24379)
                     /locus_tag="IW797_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1223798.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24466..27144)
                     /gene="mgtA"
                     /locus_tag="IW797_001010"
     CDS             complement(24466..27144)
                     /gene="mgtA"
                     /locus_tag="IW797_001010"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ1223799.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            27803..28240
                     /gene="ndk"
                     /locus_tag="IW797_001011"
     CDS             27803..28240
                     /gene="ndk"
                     /locus_tag="IW797_001011"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ1223800.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28262..29128)
                     /locus_tag="IW797_001012"
     CDS             complement(28262..29128)
                     /locus_tag="IW797_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1223801.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29288..31381)
                     /locus_tag="IW797_001013"
     CDS             complement(29288..31381)
                     /locus_tag="IW797_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1223802.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         9279 a         6681 c         5668 g        10380 t
ORIGIN      
        1 cgttattttt ttatcacaaa aattatctta cttattgtaa ggtaaagctt tttttaattt
       61 attgcccgat aatatataga aataaggtct agttactgtt ttttttaaat gattaataaa
      121 taacaacttc tttctatgaa attgcaaagc attcatcttc ttttccgcta acgatatatc
      181 cgtcagtgct tggtacactt tactaaaaag tatgagattg ccgatatctg gataggtgtg
      241 ctatactaca gaaaaagata tacaatctgc caccgggtag gataaagagg atgatcattc
      301 cattaggtct ttgaagggat gattttcctc tttttgtgta tttttttata gttaaggagg
      361 aatgtaagta tggcatggat ggaattaatc atagcaggaa ttttggaagt attctggtca
      421 acgatgatga aatggagtga tggtttttct aaaatcaatt attcttttta cacagtgatt
      481 ggcatgattg ctagttttta ctttttgtca aaggcaatca aaagtttgcc gatgagtctc
      541 tcttatccta tctggacagg gattggcgca gttggttcgg tcataattgg tgtggtcttg
      601 tttcatgata agctgaatct ttccacttgg ttctttgtgg ggatgctact catcagtatc
      661 attggcatca agattactag cggccactag acacttggaa taagtactga tcagaaaaaa
      721 taagcctttt tatttaatat tcaatttttt tatgaaaaaa taagaaataa gtccaatgac
      781 ctagcgactt ctccactcat caggtgtatg cttgtactat ctatagaaaa ggagtgaata
      841 tttttgaaaa gtctttcggt ccaagaatta tccaagaact ttggtcacaa acaagccctc
      901 agcgatgtca gttttagctt atcccctggc aaaatcgttg gattagtagg tcctaatggc
      961 gctggaaaaa caacgatcat gaaagctatt ttaggactga tccattactc agatggaaca
     1021 atctgtattg gtgaaaatga gatttctccc tctagtcata aagggttgga acaagtagga
     1081 gcattgatcg aatacccggg tatctatcca ttcttaacag gctatgatca tctgaagtta
     1141 ttctctgaaa ccaacgatgt atctgctatc gacactatcg tgaatcagct caagatggaa
     1201 aagtacattc acaaaaaagc gaaatcctac tcgttgggaa tgaaacaaaa attgggaatt
     1261 gctcttgcct tactgaacca tccgtctatc gttattttag atgagccgat gaacggtttg
     1321 gatccacaag caacaagaga tgtgcgaaaa atcattaccg acttggctac tcagggcacc
     1381 tctttcttga tctccagtca cttactcagc gagcttgaaa aaatggtcga tgagctgatc
     1441 ctgatcaatc aaggaaagat catcaaacaa tgtacaatgg ctgaattgaa ccaaacagcg
     1501 caagatttca tcgttatctc tacaacaaat gatctagctg ctcgaacgat cttgacagaa
     1561 gctggatata ctttggaaga tcctgataaa atcaagcttt taaaagaaaa cgaagaatta
     1621 ttaggaaccg tgatccagct attgtctgca caaaatatcc atgtgacaga cgtccagcat
     1681 ctgacgaatg atttggaatc ttctctattg gaaattctgg attcagtaaa gggggaagaa
     1741 gaaaaatgac attgcttgta aaacaagaat tatttaaatt gatcaagaaa aagtcaactg
     1801 ctgttttatc tgttctatta gttgttttgc tcataggaac agcacttcta gccaaaaaat
     1861 acacgacgat cattgatcca gttgaaatga ctgctcagct ttttagtgcc acatcttgga
     1921 ttgtctttat catgattgca gctgcaagca cgatcatttc gatggaagca caatatggta
     1981 cattgaaaaa tctcctttat cgaaagtatt ctcgaggaga aatattagta agcaaatgga
     2041 tcacattagt tatttattct gtttatttat accttttagc tattattgtc actgtgttga
     2101 tgaaactgat tcttttccct tcgatttcct tcacggagaa ggtttctact ggtcaaacac
     2161 tgatccaaag cttgttcact tatactttgg gtagttatat cggtttatgg ctgattttaa
     2221 gtttagtgtt gatgattgct tgctttatca atagttccgg cgcatccatc tcagcaggaa
     2281 tcgtctttta ttttgcttcg tcaatcatat ctggtatcct tattgcactc atccaaaaat
     2341 gggagtggat caaatggaat ccgatcagta tgttgaatct gcaaaaccaa attggaaacg
     2401 aagagatcat gaagcaattg actcatcttt ctactaatca aatgctattc ggtaatttag
     2461 cttacatcgt tttgttctta gcattaggat atctcgtatt taagcgaaaa aatgtatgat
     2521 gactcatcaa aaaagaggct gtgaaagaga cgttcagcct tattgccgat tggctgtctc
     2581 ttaaacaccg tttattctga aaaaggagct gtgacaacat ttgtgtcaca gctcctttcc
     2641 tagtatgttt ttgaataaac gacaggctca caacctctct ttatacttct tttatacttc
     2701 tatacgtgaa gcatcccagt ggcaaataaa accattgcaa tcactccgac catcgtcaca
     2761 cctataagta aaatcttatc taagtggata tgttgtacag gctcgttttg ctccttacgt
     2821 gctttgaggt aaaaataaat gcctggcaag aaaagaacaa gaacaagcat tacttcatta
     2881 aaggctacga ctagcatagc ccatacttga aaggcacttg ctaaaagccc tactaaaaat
     2941 tggacccatt ctttccgctg ataagaaagt ttcatttggt acaatcctaa gaaaagccac
     3001 gtgaagaaaa tagctgctgt accaagtgtg taagcaaagt tataagcact gtcagtgaat
     3061 aataatgaaa gtaaaaagac attcgtacat aacgtcgtca atacgatagc ataagttggt
     3121 gccccatttt tgttgagttt tccccatgct ttaggcaaaa gtccgtcttc agccatgttt
     3181 cccattgttt caatcggtaa gatcgtccat gagagccaaa caccaactaa agaaatgatc
     3241 aaaccgccgt tgatcacgac tgctccccat ttaccaacca tcatttcaaa cacatagccc
     3301 atagcaggct gcgccttgat cgtttgcagc tgattcatcg tcattgtccc atatggaagc
     3361 atggaaacca agacgtaaat aataatcaag ccgaatatcc ctagaatcgt tgctttccca
     3421 acatctgatt gtttttttgc tcgtgaacct acgacagagg ctccttcaat accaacaaat
     3481 acccaaagca tcaccatgat gcaactcaat acttgactgg aaaacgggat cggctgtcca
     3541 ttcgcattca atataatgtc gctagaaaag ttatcaatga attgttggat cgaaaacgtg
     3601 ctgatagcta ctacaataaa taaaaataat ggcactaatt tacagaatgt gacgatagca
     3661 ttgacgaaac tagcattttc gactccccga ttcactagcc atgcatatcc ccataaaaag
     3721 actgtactca tcacgatcga tgccagattt tgcccacctt caaatactgg gaagaaatat
     3781 cctaatgcgc tcatcaacaa tgttgcaaaa gctacgttgc ctaaccagca tgtgagccag
     3841 taaccccaac cgctgataaa accagcgaaa cggccaaaac cttcttctgc gtaactaaag
     3901 atcccgcctt ttaaatctgg acgtttttta ttgatattat tgatcgacaa ggagagcatc
     3961 aacattgctg ttcctacgag tacccaactg aaaatagctg ggccgccagc cgatccggag
     4021 atgtccgtca taataccaaa tacgccacct ccaatcgctg aaccaattac taaggcagca
     4081 agtggaatta aaccaattcc ttttttctgt tgctctgtat ccatgtaagc atcctccaaa
     4141 ataaaaccgc ttttatcaaa aagtataaca aacctttttt atttttactt aattaataaa
     4201 aactttaata gaaatataat tctaccggct tttttctcat tatacaagca aagcgcctag
     4261 tccctttcca aattacacca ccaaaaaact taaaaaagat gctatactaa aaggaaagaa
     4321 ttggaggatg atccatgaac attttatata tatcgatttc aggaaatacg cgtgcctttg
     4381 ccaaacatct agcagaatat gctgaaaaaa tgcatgcaga agatcctgtc aatcctgaag
     4441 ttactttaaa agaaatccat gaaaatagtg attttacgaa agaaacagaa ccatttttta
     4501 ctttcgttcc tacttattta gacggcggaa atggtttaga taacggtgat acagaaatct
     4561 tgaccgaaac gatgcgtgaa tatctggaac atgaagacaa tcataaactt tgtcttggtg
     4621 tagtcggtag cggaaataaa aacttcaata atcagtattg tctaacagcc aaacaatatg
     4681 cacaaacatt tggctttcct ttcttggcag attatgaatt acggggaaca ccatctgatg
     4741 tgacacgaat ttatcagatt ttagcagaaa acaataaata ataaaaaaga agaagctgaa
     4801 aaatagattt cagtttcttc tttcgtttct attgtttcta tcatattaag ctttgacatc
     4861 catcacaggt gcttttgctt ttgctttacc tgttttttcc aagacaaagt tttttacacg
     4921 atccatattg gttaccaaga cgatcatgga tgtttctttg cctgctgctt tgatcgcttc
     4981 taagtcagct tctgctacaa gcgtatcagg tgtcacttgc tgcccttctg ttactttcac
     5041 atcaaatggc gtaccgccta agtcaactgt gttgattccc atatgaagca agatttctaa
     5101 accatttggc gttgtgatcc caatagcgtg tttcgttggg aagacagagc tgactacccc
     5161 agtaactgga gaatagatat gtccgttttc tggttctaca aagtagcctt cacccatcat
     5221 cttttgtgca aaaactggat cacttgcttt atctaaagaa acaactgttc cgtcagcaac
     5281 tgatgagagg atagtcacac tttcagctga agtttgaggt actacttttt caggcacttc
     5341 atttactgtg gctgtttcag tcgctaattc ttctttttct aaacggtctt taggaatacc
     5401 gaagaagaat gtagcaacga agccaccagc ataagctgcc aataatccaa ggatgtagcc
     5461 taaccattta ccgtcagaga tcaatgggat caaagcggct cctgaaggtc caatagcgat
     5521 agccccgaca tttccgatca ttccgatcac tgccccgccg atacctccac cgatacaagc
     5581 agtaataaat gggcgtccta gaggtaatgt tactccgtaa atcaatggtt cgccgattcc
     5641 taggatcccg actggtaaag ctcctttgat catttctgtt aattctttat cttttttgca
     5701 gcgaatccat aatgccaatg ctgcacccac ttgtcctgca ccagccatcg caagaattgg
     5761 aagcaatagc gtacgaccag tttcatcaat catttgcaca tggattggag tcaaaatttg
     5821 atgcagtccg aacataacca tcggcaagaa taatgtgcct agaacgaagc cggcaaatgc
     5881 tccaccaacg ccaagtaccc agttgatccc accgaccatg ccatcagaaa tgaaacctgc
     5941 taaaggcatg atgaagaaaa tttcaatcag accgatggca atcaatgaaa gagttggtgt
     6001 aacgatgata tctaccgcat caggtactac tttatgcagt tttttctcaa cgattgacaa
     6061 tagccaaaca gcgaagatga cacctaggat cccaccttgt ccagctgcaa gagcttcacc
     6121 agtaaataag tttttcagtg gtgcttccgg attcatccct gtcagcatca caacggcccc
     6181 gataactcca ccaagagttg gtgttgcacc aaaaacttgt gcagcattga tacctgtata
     6241 gataactagg tagctaaaca tcccgttttt caagatgttc atcacatcga tatattgttg
     6301 ccatgttgct gcatcaagtg ttccggctgt gactaagttt gctaaaactg cggcaatccc
     6361 tgccacgatc ccagtaccga caaatgcagg aatcattggc acaaagatat tagaaatgtc
     6421 ttttaatacc gatttgattt tagatggttt ttgtttcgat ttttgtgccg ctttcatttc
     6481 ttgtgcccgt tcgtttacac ggtcttttcc agatgctgat gaatttgctg tagctgtact
     6541 tacagaagaa agtttttctc ctaatttcac gcctgccaaa tcaaccattt gctgtgctac
     6601 tttgttcact ttcccaggtc cgatgatgac ttgaagctgt tcatcttcta ctacaccaag
     6661 cactcctgga atcgctttta attcagagac atttacaaga tcatcattga tgatcttcat
     6721 acgcacacga gtcatgcagt ggacaatgct ttctacattg cccatgccgc ctgccccttt
     6781 ataaatttgt tttgccagtt cttccaaagt cagttcgttt tttgccatgg tcttttcccc
     6841 ctcataaggt cccttttaca aatccgtttg cttcagctaa ttttgcttga gcttcctctt
     6901 tatcactatt tgtcaaaatc atgacgatag ccagcttaac attttgatct gcttcttcaa
     6961 agtacttttc agctgtttcg taatcagctt ctgtcgcttg catgatgatt cgttttgagc
     7021 gttcgattag tttttcattt gttggtttga catcgaccat caagttattg aatactttac
     7081 ctataccaat catactgata gtagaaatca tattcaagat aagtttttgt gctgttcctg
     7141 acttcaatcg agtagagcct gtcaaaaatt ctggacctgc atcgacttcg attgggaaat
     7201 ccgcgtgttt actgatttcg gcttgtttat tgcaagaaat cgttcctgtg acagcaccga
     7261 tcgattttgc gtaatctaat ccaccgatca catatggtgt acgaccgcta gctgcgatac
     7321 caataactat atcatttttt gtcaaattca gcttgattag atcttcttgc cctagctcat
     7381 aagaatcttc ggccccttcg acagctacag tcattgcttc ttctccacca gcaattagac
     7441 ctatgaccat ttccggttct acaccgaatg tcggtacgca ttcagcagca tctaacacac
     7501 ctagtcttcc acttgttccg gctcccatat aaatcagacg tccacctttt ttaaaggact
     7561 caatcgtgtt agcaatcact ggttcgatac ttgtcagttc tttttcaacc gcagcggcca
     7621 ctttctgatc ttctttgttc attagttgaa cggcttgaga aatactcatc tcatctaatc
     7681 caaaagtttc ttggtttctg cgttccgttg ttaggttctc taaattgatt ttgctcattt
     7741 ttatcttcct tcttttctta taaattgata gttcatacca gctttgattt ggttgatcaa
     7801 tggtagctct tctttgataa ctttagctac tacattcact ttttcatccg ctggcaaatc
     7861 atatttggtt aattggatct cacccatata gcgcaaatac ttttcgttat ctaaagtaat
     7921 agaccctttc atacgcgtag ctgtatttct tgctgggata ggcggaatct ctcgaaaacg
     7981 tgcatctacg cttcgtatga catctcttgc atcgtcttgc cgattcgtat gcttccctaa
     8041 tatatattcg taaaattctt catctacagg ttccgtatgg agcaagactg tctgttcttt
     8101 ttggaaagaa gcaaattgct cctgaacgtt ttctgacaga cctgcatctc cgatgtaaac
     8161 aatatccgtg ttacttgcta atagatctag agcgcctgca agaggatgga tccctcgatg
     8221 ttcttccaaa gttggcaatc cttgatatag tgggcctctc aactctgtat ctcctggtac
     8281 aaaaccttgg acaaggagac cttgaatttt gagccattga ttttttttct gataccattc
     8341 tttgtccagc cctgtttcgg gacgtggata ataattatgc caagcttcaa tctgtgagaa
     8401 atcagcttct gcttctttca attcatcgat atctttaggc gtgatcgtgc tagcattcaa
     8461 actgattttc aattgatgtg accagtcggc aatctgctga ttggaaatat gataatccat
     8521 tcttaaacct gttacaccaa ttgctttcaa aggcgttaga tcttctaatg aaaaacccgc
     8581 tcgttccaat gcctctcctg aaatatcgac catcaattct agtttattct ttttcgcttc
     8641 gcttcctagt tcgatcaatc gcttttgata tgccgaaaca tcatcttctg gtatatgcat
     8701 ggaagtgaaa ataccgacaa agccattgga tgccatttgg cggatatact gtttcttctc
     8761 ttctgacaaa gaatcgttca taaatattga aaagccaaac atattatccc tctttttctg
     8821 tatccgtctt catttctatg actagagtat atatctaaag aaacaaaatt tcaatacttt
     8881 tattaaaata attaaatttc atttctaaag gttgagttta tattatcaaa agaattgtca
     8941 taacctaaaa aaggcactgc taagcaaaag cagtgccttg aaatatttta tttaaataac
     9001 acaaaaacat aattactcat aagaaaccaa ttctgtatta tctaccatga atgtaaagaa
     9061 cccctcatta ggcatcggat gaaaataagc attgatcact cttatcaagg cattatggca
     9121 aactaacaga tagacatttt cttcgtcttc gatgcattct ttcaataaag gaacgatccg
     9181 tgcatacaca tctaagactg actccccatt tgggaatcgt acagcgaacg ctaaacgtgc
     9241 tttttgaaag ttctcatctt tgcttggcag tccatcataa tccccaaaat ccatttctac
     9301 caatcgttca tcaacggtca gcggcaagct gagacgatca gcaactgctt gtgctgtatc
     9361 tcttgctcgt tgaagagggg aatggatgat tttcgtgatt ggaacaggca aatctactac
     9421 cttttctgct agtttctgtg cctgcatctg tcctttttca gtcagcggca catctgcccg
     9481 tccgcaaact aatccttgtg cgttccaagt tgtttctccg tgtctcgtta cataaagcat
     9541 gtgattctct ccttcgttgg caaagttccc cttaaccata ccttgttgtt cttgagatta
     9601 caagggttag attctctcaa gagaaataat ctcagtagat actttatcca ggaaggtttg
     9661 atcatgttcc actagtaaca tggcaggttt cacgttcaag atcagttgtt ctaattgttc
     9721 ttgattgaag acatccaaat aattcaatgg ttcatcccat gtatatagtt cagctggctg
     9781 tgataaagat ttagccaatt ccactttttt ccgttggccc atactcatct gctcgatctt
     9841 gttatgaaaa acatctcttt ccatcccaag ctttcggagg ttgttcaaaa atgcttggta
     9901 gtctacttgg tttttctctg caaattccgc caacgttcct cgattgtctt cataattttg
     9961 gcttgcataa ctaatgctca gatgttttgg ctggtatttt tctcctataa ccttgccgtt
    10021 gaatgccccc agaagataat ggataatgga tgactttcct gcaccgttag gacctgaaat
    10081 cgccacccgc tgatgaggct cgattgtaaa atgaattggc tcgaataaca gattttcata
    10141 ccctaattga agatcttcta cgcttaaaag tcgcttatgg tgagacgctt ggctattcat
    10201 cgtcaaagaa tcgatatatt cgatatcttt taatagtttt tctttctcgc tgatctgggt
    10261 ctccatccga ttcacgattg ctttggatcg tttcatcgtc cgtgcagcat cagcacccac
    10321 tgctccttta ttcactcgtc tagattcagt atcgatgaat ccgacttgtt tctttgtttt
    10381 atctccttct cgggaacgag accattcggc tttttcagct gctgttttct ttagcctact
    10441 gacttctttt ttcaattttt cattttgagc catttcaaat tcatcacgaa gttttttctg
    10501 ttcttcatag atagagaaat tcccttgata aagttccagt tgactttttt cgattgctaa
    10561 aacatggtcc actacttcat cgataaatcc ccggtcatgg ctgaccacga tgaagccttg
    10621 ttttttcttt ttcaaataag ccgctacttg ttttctacca gagatatcca aatgattcgt
    10681 tggttcatcg attaacggga aatgagtgtc atccacaaat aaaagtgcca gtaggacttt
    10741 tgtcttctct ccccccgata gtgtgctgaa ttctctccat aagatttcag gatctgtttg
    10801 catcaattgg agctctcttt cgatttccca tatctcaaaa tccgtaatat catttaacac
    10861 gtaataggtt aaacgttctt tatcttttgt ctgttgcggg aaataggcaa attcttgctg
    10921 atggattact tgcccttgat aaggtagttt gttttgtaga atattcagta aagttgtctt
    10981 tcctcgaccg tttcgtccga taagtcctag tttccattgt gtgtcaaaat ttagatttgc
    11041 ttgttcaaat aataatgtgc cttggctgtc gtagccgaat gtcagatttt ttatttcgat
    11101 tttagacatt ttccatcacc tcgtaaaaaa agaccagtcc tattttcaga actggctaaa
    11161 gaggatgcaa gggttggata cacacaaaaa gacccataca tcgaaaaaac caagccaatc
    11221 ccgaaaatct gcacactgct tccttgtctc cacaaggtac agtctgaatg cagctttcat
    11281 caatcgactt aatttttcaa tgcacggatc cctcactttg tcctatattt gaaattacga
    11341 tagcataaag aaaatgattt tgtcaactac ttcgggagga tggtgaaaaa tctgctaagt
    11401 gtctgtccta agatttttgt tttttctaat gtttgcctgt gttccattgc ttcttctaaa
    11461 cctattggac ccaaatgaat acttaagaca attgggagaa aatcagaaag tccttctagc
    11521 tcttggtctc tacttccgca aatagcgatt attggttttc gatatttttt tgcaagcact
    11581 gctacaccgt atggaacttt tccgtggatc gtttgctgat cgatccgccc ttctcctgta
    11641 atgatgacat ctgcttgttc gatctttttt tccaatccag tgatttggct gactaaatcg
    11701 aaaccagaaa ccatcttcgc tcccaacaaa aaaagggctc caccaatacc gcctgctgct
    11761 ccacttcctg gaatatcatc taaatccacc tcccagaaat cttttatttg ctgagcaatc
    11821 tttcctgctt gtttatctaa gaaagctagc gtcgtatcta ttcctccttt ttgtcttcca
    11881 aattgccaag cagctccttg ttgacctgta tatggattgg taacatctgc agcgataatc
    11941 aattttactt tagaaacaag aacgtttttt cgatcaaaat gaagtttttt agtagataat
    12001 aaaggatttc cttctgtgta cccttctaaa gaagcaccca aggattggag taatcctaga
    12061 ccaccatcgc tacacccgct tcctcctaaa gtgacaataa tttgttctac ttcttgaagc
    12121 agtgcatctt taataagttc tcctaaggca taactggaag caagtcggat agtttgatcc
    12181 gttggtttat caagtagatc taaccccaat gcttgtgcag attcgatgac tgcggtgttt
    12241 tttccttgcc aagttgtcaa taaatagctg atttttcgct gacggaataa gagatccatc
    12301 ccatcaatgg tacgccatgt tcccctgagc gcgtaataga ttgcttctgc agtattttct
    12361 ccgccatctg caatcggcac attgattttt cttcctttac gatcttccca gccatttaga
    12421 actgcctgat ttagttcaaa tgaagaagca cttcctttaa atgaatcaat tgccgccaaa
    12481 atatccatct gtatccctca ctttcttctt aagtaagaca ctttttttca tttttttcaa
    12541 acaaaacaat gctattttct tccatttttc tatatactaa aagggaactc attgaataaa
    12601 ggagagaaaa aacatgaaaa tcgcaatagc tggggccgga gcaatgggca gccgaatcgg
    12661 attgatgcta catcaaagcg gaaatgaggt cctcttgatc gatcgttggc ctgctcacat
    12721 tgaagccatt cgtacaaatg ggttgattgc tgactttaac ggtaaagaag tggtcgcaaa
    12781 actgccaatc tattcaccag aagaaatcat cgaaagcaac gaacacgtgg acttgatcgt
    12841 agcattaaca aaagccaatc aattagatga tatgttttgt tcgatccaat ccattattac
    12901 tgacaatact tatgttttgt gtttactaaa tggattaggt catgaagatg tactggaaaa
    12961 atatgtgcca aagaaaaata tcttatttgg gatcacgatg tggacagctg gtctagctgg
    13021 tcccggaaaa gtcacattgt taggtgatgg agaaatcgaa ctagagaact tggaaccaga
    13081 aggcgaagca tttacaaaaa aagttgtaga agtctttcaa gaagcaaatt taaatccgat
    13141 ctatagccat aacgttcgtt attccatttg gcgaaaagct tgtgtaaatg gaacattgaa
    13201 tggtttatgc acgattttgg attgtaatat tgctgaacta ggcgcgcaaa aagcggcaga
    13261 gagcatggtt cgaacaatcg tatctgaatt tgcttctatc gcagcaaaag aaggaatcat
    13321 actagatcaa gaagaagtgt accaacatat tgcttctact tatgatcctg acaacatcgg
    13381 gttgcattac ccatctatgt accaagacct gatcaaaaac catcgtttga ctgaaatcga
    13441 ctacatcaat ggtgccattt ggcgaaaagg acaaaaatat gatattgcta caccatattg
    13501 tgcctttttg acccaattag tccatgcaaa agaaggaatc cttggcgcag aataaaaggc
    13561 tctctgatga gaatcagctg aatcagcagc tggttctctt tttttaagcc caataaaaga
    13621 tcaaaaagaa aacacaactc tcatgatgcc ataagaattg tgcttgtttg tttcagtaca
    13681 taatctcttc aaaatgtagg ttttttattt gttttcaaca gaggagaggt catattcgta
    13741 ttgctcataa atagtggaat cgttagtgat aaatcgtttt gcccaatttt ctgcatcata
    13801 aaattccaat agctctgata ggattggctt aaatggtgta tgcagaaatt ccggtaattc
    13861 atctaaatca cgttctcgaa gcaaaaaaat attttcagga cgataaaaat gataatgagc
    13921 aatcgcttga ttatccgtca ctagtttctt ttgaaaatac atggattcaa atgtccgcaa
    13981 agtcactcct tgctgaccct cccgcaacag ttccagaatc ccgcgcgatt cccctgtttt
    14041 tttcaggtac tcttcataag taagatatcc aggttgttca ttccctcgat gtggaagaat
    14101 gaaatagttg gtcgtaacat ctctttttat aaattcctgc ttatagcttt ctgctcgttc
    14161 ttttctccca ttattcgttg caccaaaaaa tagatccgtc tttggttcgc tagaagaaaa
    14221 attcatatgt ttcgaataaa atgaactatt atgtttcagc ccaagttctt ttgcttccaa
    14281 aaaatcaaaa tgataaatct catcgattgc cggttcatct tgtgcctgct tcagcatcgc
    14341 ctcgtgttct gctttgaagt gattccaaaa aaataacaca atttttgcat tggtaaatgg
    14401 gcgcacgcgt cgagccatct ccatatcaaa tccataatct aaaaaaacga tcagctgata
    14461 atttgccaac tgttgtctag ctgatttaat gaattccgac tgtagtaaat cttttccgga
    14521 ccaaacatct aaagagcgga tggaaggcaa aaaataaagt gatttatttt ttgcaactag
    14581 cagtacgtca tttttgttca tcttttccgc ctcactttct ctgtcacttt ttcctatgat
    14641 agcctgttta aatagtaaaa agccagtaaa acgtttccta tatttgtatg aaaatttaac
    14701 atttgtatga aaatatgatg aaataaatca ttcttctatt aaaattcgcc taaaagtttt
    14761 ctgaatagtt gtaattgtct gaaaatttgt tatcattagt cattgttaaa tattgctatt
    14821 tattcttaat ataaattttg attgtgaatt taaaagaaag aggtcattta tggaagataa
    14881 aaaattgaaa aaacaactta gctccagaca tatcaccatg ttagctttag gtggagcaat
    14941 cggtgcaggt ctatttaaag gaagtggcga agcgattggg atcgccggcc cttctgtatt
    15001 gattgctttt ttgattggtg ggctgatttt atatatcgtg atgaaaggtc tgggaaaaat
    15061 cgttttaagc ggtggcgaca cgcatcacgg attatctggt ttgatacgcc cttatctagg
    15121 tgctcattct gctgatttta ctgattgggt ctactggtct atgtggatta tcaatatcat
    15181 cgctgaagct gttgcagctg cttccttttt gcagttgtgg ttcccgcaca ttccagcctg
    15241 ggtatttgtt tttatgttgg ctgttttaac tacgatcatc aatctttatt cggtacggtt
    15301 attcgcagaa acagaatatt ggctggcttt tgctaaaatc agtgtcatta tcttactgat
    15361 catttttgct acttatcttg tcggtcagca aatgttgggc acaggcgttt tccctaccct
    15421 tcagcaattg actgaccatg gtgggtttac tcctcatggt atgaagggaa tcatcagttc
    15481 tttgcttgta gttatctatt cttacggcgg ttctgaactg attgctatca cggtaagcga
    15541 agcagatgat cctaaacgtg cgattcctaa agcaatcaaa ggtgtcatgg gaagaatcat
    15601 ttccttttac atcattcctt tgtttttgct tttgattctt tttccttgga atactttagc
    15661 aggcacgaat gtcagtcctt ttgtgatggt atttgaaaaa atgaatatcc catttgcagc
    15721 ggatatcgtc aattttgtga ttgtccttgc tttgttctca tctatcaatt cgggtgtgta
    15781 tgcatcttca agaattttgt tcttccgttt gaaggatcga aaaggttcct ctagaaaact
    15841 agctgtttta aacaagcatc aagtaccgca acgtgctgtt ttattttgta ccagtgttct
    15901 ttatcttggt gtagctcttt cttattttgt cggtgacaaa ttatttggct atctggcagg
    15961 ttccctttcg tatacagtat tattgatctg gatcattatc agtgcggcat cctttgtact
    16021 ggcactaaga aaaggaacat tatgggataa aggaatcagc ttttttgcat tagctgtctt
    16081 aggattgatt ttccttggta tcctgttcac gaactccatt ggggtaactg tattgactgg
    16141 cctgctttac ctgttcattt atttcagtta tcaaaaaaag aatgatgcgt tcgtattgac
    16201 gaatgaatga aatcgataga aaattttatt ttatttatgt ggaatagatg gagctattga
    16261 agaaaggagt tgtgacaaaa gtcttgttgc aactcctttt tccgaataaa cagtctcatg
    16321 acctcttggt aaacggtgtt caaaaaccct ctgtgcaaaa taattgattg ttctccatgc
    16381 tcatgacatg atagaagaaa ggaatgaaaa aaaggagttt gttttaaatg aaaaagtatt
    16441 ggaagttttt gttgatgatc ggtgtttcaa cggtagttat gtttggtatg atgtacttga
    16501 atgtctatgc gctcgatcat atcttcttca gtcgtacacg agtattcatg gcgttgatga
    16561 tgggtgcagt catggcagtc atcatgctgt tgttcatgtg gaaaatgtat gaaaacaaaa
    16621 agataaatgt tatgatctta gccgctagtc ttcttgtttt tgccggttcc ttgtttatgg
    16681 tacgaagcca aactactgtc aacgacacag cttggatgaa ggccatgatt cctcatcatt
    16741 ctatcgctat cctggcaagt aaacgagctg atttaaaaga tcctgaagta aaaaaattag
    16801 cggaagaaat cattcaggca caagaagaag agatcgacaa aatgaaaaaa atgatcaaag
    16861 aaatggaatg accattatcc gtccaaataa aaaaagagga tgttccagaa gttatcagct
    16921 tcaagaaata agaagaagct acttctggaa cgccgtgaat aaggggttgt gaaaaagctg
    16981 ccctgcatca agtaataaga acagccaaac aaaaatagct tctcctattt ttcgtttggt
    17041 taggcttatt accgcagatg caagcttttg aacaccgttt attaagaggt tgtaaaatca
    17101 gcgttctgac ctgagtatta gagagacaaa tggaaaaata gttcctcata ttttgccatt
    17161 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tatcgagagg ttgtgaaaaa
    17221 gtcgcttagt ttcgcgtatt aagaaaaatt ttgtgaaatg gctttccaca tttttgtata
    17281 aatttggctt aataccgaga aactggcttt tgaataccgt ttattcgtaa aagggattgt
    17341 gacaaaagtc ttgtcacaat ccctttttat gtttttattg ttctaattgc gctgtcttgc
    17401 ttactgcttc ttgcggtttc acaaattttt ttgagattcc ccaacctgtg aaggcactga
    17461 ccatcgaaaa cacaggagaa agcaaactga aaaatacgaa tggcaagtaa gtgagtgtag
    17521 gaataccaaa tgtacttgct acgaaacttc cggcaattcc ccaaggcact aagtagttga
    17581 ttactgttcc accatcttct agcgttctac ctaatacagt tggatcaaat cctgcttttt
    17641 cataagtttc tttaaatgca ttaccaggca gaataacaga taggaattgt tcaccaataa
    17701 aaatatttac tccgatactt gtcatcaatg ttgtaaaaat caaatttggt gtgttcgtca
    17761 tctttcttga tactcgttcc atcacggttg agatcaagcc aaattcgatc aacaatccac
    17821 ctaatgacaa agttagaata atcaatgaca cagtaggcat catactttcg attcctccgc
    17881 ggctaagcag tgaatcgatt tgttggtttc cagtatttga aacaaaccct gattcgatga
    17941 ccccagccaa cttcgttaag gaagtttgtg ggttttgaat aaaaatcatt cctactgcta
    18001 cagcgatatt caacaaaatc gttaaaattg ccggcacctt tttccatgca cacagtaaca
    18061 ttaaaaataa aggaatcagt gaccagattg aaatagtaaa gtttgcttct agaacatcag
    18121 tcacttcacg aatagatgaa agacttgcgg ctttacttgt gtgtcctaaa atcatgaata
    18181 aaaagaaaga aacaacaaat gccgggacag ttgaccacat catatgcttg atgtgtttga
    18241 acaaatcggc atcaacaata gctgctgcta gatttgttga ttcggataat ggagacatct
    18301 tatctccaaa aacggcacct gaaacaatag ctccaacaac cagcgctgga tttaagccta
    18361 gtgttgtccc aataccgaaa aatgcaatcc caatcgttga cataacagta aaggcactcc
    18421 ctacagcgct acctacgatt gcacacacaa caaatacaga agggacaaac cactggacac
    18481 tgatcaattt gaacccaatg accattagag ttggaatgat ccctgcttgt atccatacag
    18541 caatcaatga accgactaaa ataaaaataa agattgggat gattcctggt ttgatgccat
    18601 tgatgattcc ttcgtgcagc caatctgtcg ggtatctttt tatcactgca tacaacatga
    18661 ccattccgat cgctgctaag atcgtgatat taggtgagac ctttaatccg acaacgccaa
    18721 tcgcaatgcc taaaacaata accattaata caagtgttgc ttctaaaaaa cttggtgttc
    18781 ctttttttcc catgataatc tctccttttt tagttgttct tatgttctaa aaaagttaag
    18841 atatcggatt actccgccgc aattcatccg cgattcctcc tttataaaaa taaaaagacc
    18901 tgttttaaac taaggacgaa tgactccgcg gtaccacctt aattaaaaac agatctgttt
    18961 ttcactttca aacttttatt caaatgtatg gactttccat gtaattcgtc aattaggctt
    19021 gatcggttca cagcgaccac cgactttctg aacaaaacac ctaaccgcta ctgcgtgaaa
    19081 agttgacttg tacgaagttt accttccata agatcatttg tcaatgaaaa agaaaatatt
    19141 ttttcatttt tctgaaaatt atttttataa ctttcacaca caagtttatt ttttactaga
    19201 aagtagcgcc ctcttgtttt tttcttgcta ctatttcttg atgttcttgc ttgattcttt
    19261 ctaatagctt tggctctttc aacagatcaa gagctgtatt ggcgagtagt tctgctgcaa
    19321 tcgcaataga agcaaggcct ttgtcacttt tagcagcagc tttgaattct tctgtgtgtc
    19381 cggcaatata tgtctcagag attgctaccg taggctggat tgttggtatg acttggctga
    19441 catttcctac atcagaagaa ccgagacttg ttctttgttc cgtttctatt tcttcatctg
    19501 gaacacctgc ttcttttgca tgctggaaga ataagtcatc aaaagaagga gtaacgatga
    19561 catcatcaac tgcattttgg aacaatccaa actcaaaagt cgtgcccgta cttatcgcag
    19621 cacctcgtac gatattttcc actttttcat atactttatc taatgtttgc cgatttcctg
    19681 cacgtaaata gaaacgtcca gcagcgtaat ctggaatgac gttggcagct actcctccgt
    19741 ctgtaatgat cccatggata ttgacatctt ttggcaattg caagcgtaga gcgttgattc
    19801 cattaaacac ttggatcaat gcttccaaag catttactcc tttttcaggt gctgctgctg
    19861 catgggaagc gacaccgtaa aatttgatgt cgactggatc attcgctaaa gattctgtcg
    19921 ttttcccata acgaaaagct ggatggacgc ataacgctac atcgacatcc tcaaaaaaac
    19981 cttctcgaac aaagctgcct ttagctgatc cgttctcgcc cccttcttcc cctggggtgc
    20041 catatacacg gatttctcca ccagtttcat caatgaattg cttcactatg ctggcggcta
    20101 atacggagta agtaccaaac aaattatgcc cgcatgcatg accaatacct ggtaaagcat
    20161 cgaattctgc caaaaaggca attgtgggac cgggtttatt ggaaatataa cgtgcatcaa
    20221 aggctgttcg gtgtccagct acttcttttt ctacccaaaa cctttctttt tctaattgtt
    20281 taatcaacgc tgctgaggaa taaaactcat agttacttac ttcaggatgt tcgtggatat
    20341 ctagagcaag tgcttgatat tctggtaaac gatattggat tagatccttc aattggtttg
    20401 ttgagattgt tgttgtcata aaaaattcct cctatgctag tcatttttct tattttctcc
    20461 cgaatgtttc ccatgcaggc acgcttgatc cttttgatgt tttctcgatt actttgactg
    20521 tgtcttctgt ttgataagcg tctactattt tttgatacgt tttattgttt tcatcttctt
    20581 ttcttgcaac gatgatattc acataaggac gtgcatggtc atccactggt tctaagaaga
    20641 tggcatcaga agcaggatta aatccagcat ctacagctac accgctattg atcactgaaa
    20701 tgtctacatc tgacaaagca cgtgctgttt gggaagcatc taattcagaa atgtctagat
    20761 tttttctatt ctcggtaata tcagaaacag tgggcgcttg tccttttgcc ttatccactt
    20821 tgatcaatcc agcagtttgc aataacagaa gagctctgcc gccatttgta acatcattag
    20881 ggattgctac ttttgctccg tctttgatat cagctacatc cttcaaagtg ttagaataaa
    20941 ttcccagcgg tgcattgaca gtatttccga tcgatactaa atcagttccg tgttctttat
    21001 tgtaattgtc taaaaagaat tgatgttgga aagaattcaa atcaatcgat ccatctgcta
    21061 aagccacatt tggttgattg taatctgtaa atttcacgta ttccaaatta atgttctcct
    21121 ttttcaaacg gtctttcacg ttgtcccata catctgtatc ttctccaata atccctagtt
    21181 ttacgatagt ttcttttttg tcagttgtat ctgcttttcc ttggctacac cctgccaacc
    21241 aaataattgc tacaaatgta atgactacaa aacgaataat ttttttcatt ttttctcctc
    21301 cactttcaat gtgtaatttt tttaatcagt tgattactga taaattgact taaaaaaact
    21361 aagattagaa tcaataatgt agccacaatc gttacatctg tttggaatcg attataccct
    21421 ctcgatatag caagattccc taatccgcct gcacctactg ctccagccat ggtagtcagt
    21481 ccgatcacgt tgatgatagt caacgccgag acccgaatga taccaggcaa accttctaac
    21541 aaatacacac gtaaaatgat accgattgga ctggttccca tcgcttctgc cgcttcgatc
    21601 accccaggat caacttctag cagtgcgttc tcgatttgac gagcaaaaaa cggaacaact
    21661 ccaaaaatca atggcactaa agctgctttc gcaccaatcg tcgttccaac taagaaacga
    21721 gtagtcaatg ctaaaagaga aagcaagata atgaaaggga tggaacgaat gatattgatc
    21781 agcttatcaa gtatttcaaa tagcagacgg ttagctagta atccattagg gcgtgtcact
    21841 actaacgtca cacctaagat gattccaatc atcccgccaa tgaatgctgt ccaaaaagac
    21901 atatatagtg tttgcttcgt cgcttcgaca aattcatccg gaatcaaata aacattgggc
    21961 aaatatgttt taaagaaatc agtcatttta tcttcctcct tcgatcactt tgatacgact
    22021 atctaaatcg tttttttgta aaatatttat ttttacacca gatttgatca aataatccaa
    22081 tgcttttttc cgctgtttgg tttctccagc taaagtaacg atcaaactcc ctaacggcgt
    22141 ttcttgtaat aactctacat tgccatataa aatattcgct gttacttgat atttgctgta
    22201 cagatgagcg attagaggtt cgttcgtctg atttccaata taacttaatt ccaccagcca
    22261 tttattggcg atctgatcgg caaaagcaga atgggcaatg atcgtttcta atgcttggtc
    22321 aagatgagta gctgttcgaa taaaatcttt ggtcaattct tcttcaggat tgctgaagat
    22381 ctggatgctg ctaccttttt caacgattct tccatcttcc ataaccgcta ccttgttgca
    22441 gatttctttg acaacttgca tctcatgagt gatcaggacg attgttaaac ctaactggcg
    22501 attcaatttt tttaacaatg caaggatctg aagggtcgtt ttcggatcaa gtgcactggt
    22561 tgcttcatca cataataata ccttaggttc attcgccaaa gctcgtgcga ttgctactcg
    22621 ttgtttttgt ccccccgaca gttgtttagg ataagcagat gctttttctt ccaaaccgac
    22681 aagttccagt agttcattca ctttttgacg acgttcctgt ttcgattttc cagaatactt
    22741 taaagaaaaa tcaacgttgt caaaaatagt tcggctttcc attagattga aatgctgaaa
    22801 gatcataccg atggcctttc tttcttttcg caattctttg ggagagagct gactcagatt
    22861 cgtctgattg acaatgactt ctcctgctgt tggtttctgc agcaaattca tcacacgaac
    22921 taacgtactt ttccctgcac ctgagtaacc gacgattcca taaacatcgc ctttttcaat
    22981 cgttaaatct acagaatcta ctgcctgtat ctctttttct ttttgtttga aacatacaga
    23041 aacatctcgc aattcaatca ttaaaattcc tccttatcta aaaaaagcgt cctcaagaaa
    23101 atgaaaatgt cattttcttg aggacgcttt tttgcgtgtt accacctcat gttcaagctg
    23161 tgttcacaca caactcctct tcaagtacga tcatactcta gcaagatatc ggtcgcagcc
    23221 gtcatcgcct tacatctatg ctcgggatga attgctcgat ggccattttc caatagtcgt
    23281 tccttgcttc ttctcagcta ccgaagctct ctgtcaaagg ttcttctatc gtacttacca
    23341 tttcaatgca aataatattc aaaaaaaaag tccttttact aacataaact atgtcagtaa
    23401 aaggacgacg tatcgcggta ccaccttttt tcggaagttt ccttccctca agcccaacat
    23461 ctcaaaaaga gagattggcg ttcgttttaa cggtcgaaca gttccgaatt tgcctactta
    23521 tcgacaaatt attctccaag gccattttca gcttttctta cttgactctt ctcaccagcc
    23581 agagcctctc tgcaaagttt ccaaacttac tttccttttc aacgaaatcg atattttgtt
    23641 gttgggatta atctacatga ggaaaactga aaagtcaagt acgaaattca aattattttt
    23701 tctatattta tttttctgtt tttttattta acatttcaat aacagttcga agatcttttc
    23761 cttccgcaag aagtttcaat ttttcctttt cttcgacggt ataatttcgg acaaaactgg
    23821 aatcaggtat caaaacaacg cctaatcctg ctcgtgaagc agctgttgct cctactaaag
    23881 aatcttccac tgcaaaggct gaactagctt ctaatcctgt tcgttccaaa gctgtcagat
    23941 aaggaatcgg tgaaggcttg ttctccgaca cttcgtctcc gaatgtaaac gtgtccaagt
    24001 aatcagctaa tttaaagtga tccaagatat ctgttgctct tttcgtaaca gtggtcgtag
    24061 caatgcctag tttcatatga cgttctttta agtctgccaa tatttctttt gcatatggtt
    24121 ttaagtggat ggctccgctt gtcagctgtt gataaaaata ttcttcacgc ttcgctcgga
    24181 cttcctgtac tttttcaggg ctacctgctg ctttcactaa aatatcaaaa ctttgtttcc
    24241 agttttttcc gccccaagcg tcaacgattt cttttgggat atggatatca aattgctgaa
    24301 atccccaaag ccagccatca cgatacgttt tttctgtgtc aactaacaaa ccatctaaat
    24361 caaatattgc acctttcatc tatacattaa tcctttccaa aacaatacac atagtttagc
    24421 acgactggtc caaaaagaac agaagcaaag aaccagcctt ctcgtttata agagttcttg
    24481 gaatcgacgg atatagattt tcttcacagc tgtcacaagc gccagataag cgatgactgt
    24541 agcaaataac cataaccagt aattgccagg aagtggcatc aagcctaatt cagcaccaaa
    24601 acttgtaaaa ggaaggacag aaccgacaat aattcctaat gtagtgataa caaacatggc
    24661 aaatgttgcg ttgctttgga taaatggtac tttcggtgta cgcagtgcat ggagcactaa
    24721 ggtttgtgtc catagtgatt cgacaaacca tcctgcatgg aataatgcga tgaaaatcac
    24781 tcgctgtgaa ctatccaatg tatggaaact tccgcccact acagcaggac aaataacaaa
    24841 atacatcaac aagtaagtag taatatcaaa tatcgaactt gttggaccaa agtaaaccat
    24901 gaattcccca atactagatg cattccattt ttttggctcg tccaaatatt ctttatccat
    24961 ccgatcccat ggcaaggaaa tgcaggaaac atcatagatc agattcaaaa acaaaagttg
    25021 taaaggcagc attggtaaaa atggcaggaa cgtacttgca atcagcacgg aaaacatatt
    25081 gccaaaatta gaacttgctg tagccttgat atatttcatg atattcccaa aagtttctcg
    25141 tccagaaaga aggcctcttt ccaaaatcgt caaatctttt tctaaaagga tcacgtctgc
    25201 agattctttc gcaatatcca cagctgtatc tacagaaata ccgatatcag ccactttcat
    25261 tgcaggagcg tcattgatac catctcctaa aaatccgaca gtatgaccat ttttacgtaa
    25321 agctgtcgtc aaacgtgctt tttgctgagg attcaatttt acgaatactt catggtcttc
    25381 agctacttgt gctaattcat gatcactcat ttccgagatt ttctcaccgg taaccaattc
    25441 attgatctct agacctactt gacggcacac tgaacgtgta actaattcgt tgtccccagt
    25501 cagaactttt acagctacac catgttcttt cagagctttc aacgcttgtt tcgttgtttc
    25561 ttttggtgga tcaaggaaag caagataacc aatcaaaacc atgtctgact catctttgac
    25621 tgaaaattct ccaacgacgc ttggattggt cttttgtgcg acaccaatca ctcgtaagcc
    25681 atcttcatta agtgactgta cttttgcaag tacggcttct tttcgttctt ttgttaatgg
    25741 tgtaacagta ccgccaagat caataaattt agaaatgttc agcatttctt caatcgcgcc
    25801 ttttgtgatc atttgtgttt taccagctgg atcagcaacc acaacactca tacgacgacg
    25861 ttcaaaatca aaaggaattt catccacttt ttgataattg atcttatctg tatctaaagt
    25921 ttctgttgct tcatcaatga tagccacatc taacaaattt ttcagccctg tttgatagta
    25981 gctatttaga tacgcatggc gcaaaacacg atcatcttca tttccttcac aatccatatg
    26041 atattcaaga atgattttat cttgtgtcaa tgtccccgtt ttatccgtac ataacacatc
    26101 gattgcacca aagttctgga tggcattcaa atttttgatc actgttcctt ttttggccat
    26161 cacacttgct cctttgacca aattcgtcgt aacgatcatt ggaagcattt caggtgttag
    26221 accaactgct acagatagtc caaaaagaaa agcttctagc caatcccctt ttgtaagccc
    26281 attgatgacg ataactgctg gagccatcaa tgacataaat ttgatcagta gcatggatgt
    26341 tttatggatg cctaattcaa agcttgattg aattggtttt tcagatagcg tttctgctac
    26401 atgaccaaat agtgtttcat ttccaacaga aacaatgatt ccttccgcac ttccactgat
    26461 gacattactt cccataaaga caagattttc gtaacttgtt tcgctttgct gatcgtcttc
    26521 acatactttc gcatgctttt ctactggata gctttcccct gtcaatgctg cttgggaaat
    26581 gaataaatcc tttgcggaca ataaacgcat atccgctggt atcatatccc ctgctgaaag
    26641 ccgaacaaga tctccacaga cgatttcttc cataggaatc tctgtgtact ttccattacg
    26701 ttttacagct gccgttactt tcacaagtga tttcagtttt tcagccgctt gattggaacg
    26761 tacggactga atcaacgtca ttgtcccgct gacgataacc atagtgaaga taatggctac
    26821 accgaaaaga tctttatctc caggagctgc taatacatat tccgtgatga atgaaataat
    26881 tcctaatgcg atcaacacta atgtaaatgg cgtgatgtat gctttcaata tttctacaat
    26941 gaatggtgtt tttttgccat atgaaatcgc attttcaccg taattttctc ttgaacgttc
    27001 gatttgttct tctgtcaatc catttgctga tgcatggaat agttcaaata attcttgttt
    27061 cgtttttcca gcaaaagctg catagatttt ttgtgtatct ttcttttttt ccatctcaat
    27121 ctctcctttg tgtccaaaag acagctgttc tcgctgcttt tggtctttag ctattcatcg
    27181 gatcgataga gaaaaaactg aaaggggctg agaacagata gataatagcc ctggcctttc
    27241 ctctagatcc ttgtttttcg aatgactgtc atccatttgt tctcatctcc tttactaagt
    27301 aattttaatt catgacttcc aatcattttt cgaatagtta tttttccaat tagaccacct
    27361 cctttttatt tttcaccgat ggggatttgc ggcaacaaaa aaacctctcg ccaaatactc
    27421 agcgaaaggt ttgaaaaagc atattaaaaa aagcttctca gatctcatcg ttgagttttg
    27481 acactgtaca acgtaaaaag cagatacctg cttttagcta ccttatgaaa agccttatct
    27541 cgacaattcc tgttctaccc attggcgtct ctcgacgttt ttgggcagta gcctatgttt
    27601 gtataggagc ctcacctaac aaatcttatt cggttttcct tcgttaatat aacaactaaa
    27661 aaagaggatg tcaaatcaat tttccatgtt tttcatactt tttttaaaat acacacgtta
    27721 tattcctgtc tcacgcggtt ttatttgtct cattttccac ctcccgctat aatagaaata
    27781 tagtaggaaa cgaggtgctg ttatggaaag aacacttgtt attatcaagc cagacggcgt
    27841 gagacgccat ttagtcggaa gcatcatcca acgttttgaa gcaaagggac tggccattgc
    27901 tgaaatgaag tttgaaacta tgactcctga attagcaaaa gaacattatc agcacttgac
    27961 tgagcgcagt ttttttgatg aactgatcga ttacatgact tccggtcctg tcgtttatct
    28021 tgtgctggtc ggcgaggaag tgatcgatat cgtccgtaag atggttggag caacaaaagc
    28081 tgccgatgct gttccaggta cgatccgagg agactacgcg ctgccgggga ctgaaaacat
    28141 cattcacgct tctgattccc gtgatgctgc ggtaaaagag atcgcccgct ttttcccgcc
    28201 attcgatata aacaaaccgt ctgaaaaagc ggcgaagtga tacaaattct aaactgctgg
    28261 tctagttgct gggttcagca atggccagca gttttttctc taaggacctc cgttgattct
    28321 ttcctaacat atattcgagc actgtgaaga attcttggta gtctttcgtc ttttcttccc
    28381 ctaaagcagc agaaatcaat ctttgataat aatctgccat caatgtttca tagatcactg
    28441 tacttctttc tttatgtgct aagctccgtt tcatatcttc attcacacta cgtgcctttt
    28501 tcaaccgacc attttctata taaaacgaag tcaggttcat taaaaaagta aagagatact
    28561 gctcatgatt gttcaactgt gctaatcgtc gcattctttt tttgattttc aaataattgt
    28621 tctcaatgaa ccgatcactg aatatccaag gaaaatcagc tagaatagat agttcaaatt
    28681 gaccatagtc tggtatctgc atcaagtatt cttcaatgat tcggcattca taacgtaaaa
    28741 attcaaattc tgtctcagat acttctgttt tttcgaccat gtgaagattc aaaaccaaat
    28801 aatacagatc ttctgttttc ttaaaatgcc tacggttatt gctgatctct ttataaggat
    28861 caaagctgtc ttcttgtacc cgatctagca ttcgtctttt tttctttaac tttattggtt
    28921 catccagctg gtttcgcatc cattcatatt ctgcaagtgt catgttcatt cgctctaaaa
    28981 atgatctcaa tgttacaaat gacatttttt tcactgaatt tgtctcaatc ccgactagag
    29041 aagtcttaaa acagattcct tccgctaatt gggactgtgt caacttcttg ttgactcgaa
    29101 gtttcttgat caattcacca tcatacataa tagttctcct ttttaattta aatttttcca
    29161 cattcaataa atagatacgt taaaaaggaa aaatacacga aaaaaataaa aaaatgtttt
    29221 tttcataaaa aataccaacc ttcaagcatt agaatgttgt ctaactactg aaggttggtt
    29281 tgattgttta ttgattattt ttttgctgtt caacgtctgt tcttgttact ggatataagt
    29341 tggttgttgt tttattacca ttttttgaaa agacacttgt cgaatgttcg cctttttgct
    29401 tttcgattgc ttcattacgt tccgcttcat ttttatagtc atattttttc ggatcaatcg
    29461 tctcaaatcc gtttggcaca taaaaccgca agagattttc ctgattcagc ttatctgaaa
    29521 gacttaacgc agtcttgact ttttcctgat ctttggagat ttcctcatcg attcctgctg
    29581 ctttcgtatc gatgatttct cctgtctgat tattgtaagc ttttcctcct aggacggtgt
    29641 atttgggtgt cacaaagttc ccatttcgaa aagcaactac ttgatcatgc tgagaagata
    29701 ataagtctgt accaaaatga agataatctt tatcatcgat tcccaataga tgtagtaatg
    29761 tcggcaaaac atcgatttct ccaccgtatt catgattgac tgtcccttta gtataaccag
    29821 gcatatggat catcaatggc actcgttgca tctgtgcgtt gtcaaattca tcccatgtat
    29881 ctggatcttt accaagagcg cttgctaaat ctttattatc tgtattcgag atcccaaaat
    29941 gatccccgta aatcacaaag attgaatttt ggtaaatacc agattttttc aaatacgtaa
    30001 aaaattgttc caatgattga tccagataat gtgccgttcg aacataatta tctactgtgc
    30061 tattgccagt attcaaagat ggaaagtcga aattcttgtc atccgtataa taaggcgtgt
    30121 ggttggttac ggaaagaaat ttcgtgtaaa acggctgttg tagatgctca agatattggg
    30181 cagattctct gaataaatct ttatctaata tcccataacc taacgtttcg tcggattcat
    30241 caaaataaga tcgatcaaag aaattgtcat aacctaagtt tttatataca tgatctctat
    30301 tccaaaaact agctacattc ccatggaaaa cagcgcttgt atagccttgc ttttgtccaa
    30361 ggatcgctgg tgcggcttga aagacattgt ctgacccaag ttctgtgaat aatgagcctt
    30421 gaggtaaacc aaatgtgcca gtttccaaca tattttcagc atcacttgtc tttccctgtc
    30481 ctacttcatg aaagaaattg tcaaagctga tcgttgcttg attctggaaa atactattta
    30541 aaaacggtgt gacttcttgt ccgtctacct tcatattaat caaaaactgc tggaaacttt
    30601 ctaaatggag aacgatcaca tttttccctt tagcgatccc aaatgtttcg ggatttggct
    30661 ctgcataatg tttctttgta taatctaaaa cttcatcaat cccattactg cttgcatgcg
    30721 cgcgaacact gctagtcatt cctgttttga ttccgtcata aatagtatac gcatcgattc
    30781 ctagatattt gacaatatat gaacggtcaa atgttcgctg cagcaattgt ggacggttgg
    30841 cttcgctcag cgctagattt cctgaaaaaa taagccctga aagacaggta acagcgatag
    30901 ccatcggttt gccgacaggg ttgcttttga taggtatttt cttgaccttc aaccaaacca
    30961 gtagcccaat gaacacagcg atatccagcc agtagatgac atcatggggc ttcatcaaag
    31021 aaaaggagct tcctgacaat ccttgcgaca ctttcgaaaa gcctaataca gatttgactg
    31081 taatgaaatc tgtgaattcc cgataaaaaa taatattcaa gtacaacaaa agcgtattta
    31141 aaatatcaat gatcaaaaga atagctaacg ctggctttgg ctttttaata tacaaagaaa
    31201 ggccaaaaaa caataaggta gtcgcaattg ggttgatcca caagataaag tactggatcg
    31261 taccttccac acctaatgaa aaatctgcat agtaggcaat gatcgtcttc agccagaaaa
    31321 agaaaacagc tgtcaaaacg attgctaaac gattttggca caatttttta agttgtgtca
    31381 cgtatatcac ttctctctat tcacattttt tcttattttg ctcctgaaaa atgaaaattt
    31441 cctaaaaaag ttgttatctt aagatttttt tattctttct aaagattatt ccgtccactt
    31501 ttcacagcat aaaaccgctg cgatgtaagc gattaaacta atgaagagat aaaagtcttg
    31561 tggtgaccgc ttcactccac ggtattcaaa agccagtttc tcggtattcg tacaaaaaaa
    31621 gaggctgtga caaaattctt gtcgcagctc cttatttctt gatgcaggac agctttttca
    31681 caacttcttc cttaaacggt gttctcctgc caactcctcg gccacaagtc acaatattta
    31741 taaaacatga gaagctgttt tttgaaattg ttccttgtgg ctcagagtta aacggcaggc
    31801 tcaagacctc ttgatacacg gtgttcaatc agctgaccct cggtattaga ttatgtcagg
    31861 acgctaattt tacaacctct tggtaaacgg tgttcaaaag cttgcatctg cggtaataag
    31921 cccaaccaaa cgaaaaatat aaggagctat tttttgaaat tgctccttgt ggctcagagt
    31981 taaacggcag gctcaagacc tcttgata
//
LOCUS       DACEPD010000022        31273 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.022, whole genome shotgun sequence.
ACCESSION   DACEPD010000022 DACEPD010000000
VERSION     DACEPD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.022"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..832)
                     /locus_tag="IW797_001014"
     CDS             complement(<1..832)
                     /locus_tag="IW797_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1223803.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     V"
     gene            1098..1442
                     /locus_tag="IW797_001015"
     CDS             1098..1442
                     /locus_tag="IW797_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ1223804.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1443..1862
                     /locus_tag="IW797_001016"
                     /pseudo
     CDS             1443..1862
                     /locus_tag="IW797_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1954..2304)
                     /locus_tag="IW797_001017"
     CDS             complement(1954..2304)
                     /locus_tag="IW797_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223805.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2270..3601)
                     /locus_tag="IW797_001018"
     CDS             complement(2270..3601)
                     /locus_tag="IW797_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ1223806.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3857..4423
                     /locus_tag="IW797_001019"
     CDS             3857..4423
                     /locus_tag="IW797_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1223807.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4568..4655
                     /locus_tag="IW797_001020"
     tRNA            4568..4655
                     /locus_tag="IW797_001020"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4600..4602,aa:Ser,seq:cga)
     gene            4989..5963
                     /locus_tag="IW797_001021"
     CDS             4989..5963
                     /locus_tag="IW797_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1223808.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            6288..8183
                     /gene="asnB"
                     /locus_tag="IW797_001022"
     CDS             6288..8183
                     /gene="asnB"
                     /locus_tag="IW797_001022"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ1223809.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8378..8824)
                     /locus_tag="IW797_001023"
     CDS             complement(8378..8824)
                     /locus_tag="IW797_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ1223810.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            9052..9201
                     /gene="rpmG"
                     /locus_tag="IW797_001024"
     CDS             9052..9201
                     /gene="rpmG"
                     /locus_tag="IW797_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1223811.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9289..9807
                     /locus_tag="IW797_001025"
     CDS             9289..9807
                     /locus_tag="IW797_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1223812.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9884..10840)
                     /locus_tag="IW797_001026"
     CDS             complement(9884..10840)
                     /locus_tag="IW797_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1223813.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            11006..11812
                     /locus_tag="IW797_001027"
     CDS             11006..11812
                     /locus_tag="IW797_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1223814.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11809..12798
                     /locus_tag="IW797_001028"
     CDS             11809..12798
                     /locus_tag="IW797_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1223815.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12795..13799
                     /locus_tag="IW797_001029"
     CDS             12795..13799
                     /locus_tag="IW797_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ1223816.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13931..14473
                     /locus_tag="IW797_001030"
     CDS             13931..14473
                     /locus_tag="IW797_001030"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ1223817.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14493..15191
                     /locus_tag="IW797_001031"
     CDS             14493..15191
                     /locus_tag="IW797_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ1223818.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15212..15424
                     /locus_tag="IW797_001032"
     CDS             15212..15424
                     /locus_tag="IW797_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ1223819.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15424..16386
                     /locus_tag="IW797_001033"
     CDS             15424..16386
                     /locus_tag="IW797_001033"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ1223820.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16404..16799
                     /locus_tag="IW797_001034"
     CDS             16404..16799
                     /locus_tag="IW797_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1223821.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16962..17327
                     /locus_tag="IW797_001035"
     CDS             16962..17327
                     /locus_tag="IW797_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ1223822.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17324..19135
                     /locus_tag="IW797_001036"
     CDS             17324..19135
                     /locus_tag="IW797_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ1223823.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19197..19364)
                     /locus_tag="IW797_001037"
     CDS             complement(19197..19364)
                     /locus_tag="IW797_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ1223824.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            19612..20601
                     /gene="galE"
                     /locus_tag="IW797_001038"
     CDS             19612..20601
                     /gene="galE"
                     /locus_tag="IW797_001038"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ1223825.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            20613..22097
                     /gene="galT"
                     /locus_tag="IW797_001039"
     CDS             20613..22097
                     /gene="galT"
                     /locus_tag="IW797_001039"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ1223826.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            22121..23101
                     /locus_tag="IW797_001040"
     CDS             22121..23101
                     /locus_tag="IW797_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1223827.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            23190..24014
                     /gene="tsaB"
                     /locus_tag="IW797_001041"
     CDS             23190..24014
                     /gene="tsaB"
                     /locus_tag="IW797_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ1223828.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            23998..24540
                     /gene="rimI"
                     /locus_tag="IW797_001042"
     CDS             23998..24540
                     /gene="rimI"
                     /locus_tag="IW797_001042"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1223829.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            24547..25011
                     /gene="rimI"
                     /locus_tag="IW797_001043"
     CDS             24547..25011
                     /gene="rimI"
                     /locus_tag="IW797_001043"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ1223830.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            25008..26024
                     /gene="tsaD"
                     /locus_tag="IW797_001044"
     CDS             25008..26024
                     /gene="tsaD"
                     /locus_tag="IW797_001044"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ1223831.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(26633..27334)
                     /locus_tag="IW797_001045"
     CDS             complement(26633..27334)
                     /locus_tag="IW797_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1223832.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            27795..29024
                     /gene="arcA"
                     /locus_tag="IW797_001046"
     CDS             27795..29024
                     /gene="arcA"
                     /locus_tag="IW797_001046"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ1223833.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            29115..30134
                     /gene="argF"
                     /locus_tag="IW797_001047"
     CDS             29115..30134
                     /gene="argF"
                     /locus_tag="IW797_001047"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ1223834.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            30249..31196
                     /gene="arcC"
                     /locus_tag="IW797_001048"
     CDS             30249..31196
                     /gene="arcC"
                     /locus_tag="IW797_001048"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ1223835.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
BASE COUNT        10187 a         5373 c         6480 g         9233 t
ORIGIN      
        1 ctactgaatt gaaatccata gatttcaata aaccatctct acgtaacaat ccgttagaat
       61 tctcgtttag gcctctttga gacggcgttc ctggatcagc gaaataaatg gcaatatcat
      121 tgacattact gatctgtttc caatttgaaa attcctttcc acaatcaaaa gtgatggatt
      181 tgaataggtt tttcggtaca gattcaaacc attgatttaa ttttttttca atatcaatcg
      241 cttgtctacc acacggtttc aatgtgatga taacttttga taatcgttca actaaggtaa
      301 ttacagcact tttatgtttc agacctacga tagtgtcacc ttcaaggtga ccaaactcat
      361 ttgagaattg gctataatcc ttttcacgtt catgaataga gcggcggaaa gtttgttttc
      421 cacgtctttc ttgatgtcca ttcggtttac gcttgccttt catcggtaag tcagaagaat
      481 caaatagccc ctttttgaac atacgataaa tggtacgagc agaacaacta ataggaaacg
      541 ctgcacgacc aacaatcaca tcgggtgtcc atccttgaac aacctttctt tgaatatatt
      601 ctgattgttc ctcgggtaaa acaagcggac gtctaccaca gtttgatttg tttttcttat
      661 actgttgata atattctaag gctgattttc cttgcttgaa gaacagatat actttatgga
      721 tcgtttgtct tgaacgattc aagcaatggg cagttttagc aacagattga ttcattttga
      781 aataagactc tattattaca agttcatccg ttgtaagatg ggtataggtc atttgtactc
      841 actccttata attctttggt cggaactatt ttgagtgtag cacaaatgac ttttttagtt
      901 gtctagctta attttacaat cggcgaataa aaaaagcgtt agcaactatg cttaaaaagc
      961 atatcgactg aataaactaa gcattggaca ggctgaaaga ctacatttaa aatgaaatgc
     1021 gaagaaacaa tagaaaataa agttgcctta gaaccatagt tgaatcacct aacagtcgtg
     1081 aagggagaat aagagaaatg gcagaaaaac aaaccgcagg acgagatcaa ttaggagatt
     1141 ttgcaccaaa atttgcagaa ttgaatgatg atgttttatt tggacaagta tggtccagag
     1201 aagaattatc tgctcatcag cgtagtatca tcaccatatc tgcattaatt tcaggaggaa
     1261 attttgaaca attgccagct catctgaaga ttggcaagca aaatggcatt actagtgaag
     1321 aaatagcaga aatcattacc catttagctt tttatgtagg atggccaaaa gcttggtcag
     1381 cttttagttt agcaaaagaa atctatttag aagaaaatag cgaagataag aaagaggcgt
     1441 aaatgacgac aaaacacgaa gaaatacaca atggcgtgat ttttccagca ggtgaaaaaa
     1501 acgaagctta cgctcaagta tttaccgggt aaagctatct gaatatgtta gtgaatgatc
     1561 ccgaagtttc tgttggtgta ggtaatgtaa cttttgaacc aggttgtcgc aatcactggc
     1621 atgtccataa agacggctat caaatcttac ttgtgaccgg aggagaaggc tggtatcagg
     1681 aagaaggaaa gccagcaaga cgattaatgg ctggagatgt cgtcgtaacg cacaaaggga
     1741 ttaaacattg gcatggtgcg acaaaagaca gttggtttga acatttggcg atcactgctg
     1801 gtaattcaga gtttttagaa cctgtaacag atgaagtgta tgaaggattg gaaaatcaat
     1861 aatgtcaagc ttgaacagct cctgaaagta gattttaatc gaactttcag gagctgcttt
     1921 tttgattcag tcttttataa atagaaaata gggttaataa taggaatgag aagcacgtcg
     1981 aaagatctta atattgataa tcggctgggc atacatgatt tccacgtcat caatcaaact
     2041 acctaacaga tgataatctt cccgatcatg tcggggatta cctaatagtt cttgataata
     2101 gatttccata ctagggtccc gaggattgtt cagcaaatca gcaagtacat ctaaatcttc
     2161 tggctgagtt tctgccgtac cctgtatctc tatgaaaagc ccttcctcag caaattctac
     2221 tccaatacgt atttcagtta tttcatggga atataaataa gtaagaagct catcgatgat
     2281 tctttttgat aattcttttt ccaagtgtct ttttttcctt tctgacagcg gtaaagttat
     2341 ctagtatcgg tacaaggata cctgcaacga aaccagttga aaaaccactg ttatatagat
     2401 ttagtcctcc gtgcaaagtg ctgacgttat ggacaagagc aatatgtagt acaccagcta
     2461 ttacaccgta aaaactgccg taaaaaccgc ttatgggcgc gagacctgtc ccaaacacag
     2521 ctgcaacaag catgccagta ctggtgatct catgaaaagt tccaaacaaa gaagcaataa
     2581 atattccgac tagtatcgga aaactgtttt ttaaatgaca accaaaagca ctgaaaccca
     2641 ccgcagataa aatagcaccg atgacaggtc catttaactg accgccaacc agcaaaacat
     2701 aagaagacag catcagacca actaagccta tgttgatcaa agtagcacca ataccagaat
     2761 tagcaatgaa atcagttgtc aattttccag atgaatcgaa aatttctcga atccccaaaa
     2821 gccgaaagga attataatag aaaccaattc cgactgtcag cgaaaaaaag ccaaaaataa
     2881 tccaaatgag aggaaagtga tattcattga aaaccaatgt attttcgacg attgaatagc
     2941 tgaataacct caaaacagca gtaaaagtca tcgcaatcaa cccagaggtg aatccaatat
     3001 tataaagtgt gaatccacga tggaaaacta aagtttgagc agctaagggc ggaagaagta
     3061 gaccaattgc tatgccagct aaattcccca ataaaatacc tatcagtaaa ggaaaacgca
     3121 taccaaaagt gatataacta acgaccggtg ataatgcgct accaaacagt ccgatcatga
     3181 tgtactgaga aaaaggttta ttgacaaata cagcgtatag atacacacct aaaatgattg
     3241 agatagaatt ataaaggttt ttgccaaaaa aagagaaacc ggtaactgtt agaatcgaag
     3301 caaataaggg accgtttaaa atcaccttgt gccgataagc taaaagcaca gaaagtaagg
     3361 tcaaaatccc tacatttatg aatgcgcttc cgaaaccgcc taaagcaaaa taatctgtca
     3421 aaagattgct tggcgaggta agaatagtta gcaatccact ccaattatca gtaggagtat
     3481 tagaaagcaa gccaataaaa agaaaaaaaa cacctgtaaa accaagataa aaatactttc
     3541 ttttctgtga aacgattcgt ttttgaggat gaatataaat aacgtcagtt tcgttttcca
     3601 ttacacacct tctttttcat tttctttaac tataacttga aatgaaagat ttggacaact
     3661 atcatttagg tcaaaactaa aaatgtaagg gaacccattg aatttaacca aattttttta
     3721 attttgcaaa aaaaagttaa agaagactga aaaaaaattt aaaatataac gttttgagta
     3781 aaatgtttgc aaaaaatgct aaaaataggg tacaataaaa caacatatac tgcagtaaag
     3841 aagtggggga gttttaatga gaagaaaagt ctataccaag gatcaaattc taaaagctgc
     3901 ttatgatgtt gttgcaaagg aagggtttaa aggatttact gcgagaaata ttgctaaaaa
     3961 gatggggatt tcgacacagc cgatttatct ggaattcaaa aacatggaag atttaaaaaa
     4021 tacgttgctg gattctatct ttgaacaact aaaagaagat gttttcccta ttgaacgtac
     4081 tggtgataaa atcattgatc tcggtttgaa ctatatctac tttgcaaaaa agcatcatag
     4141 tctttacatg gcattgtaca ttgatgaaca tggggacgga aagaaaatgc atgatttttc
     4201 atacaattat ttccgtgaaa tagttaaaga agatgaaaga tacaatcaat tatcggatga
     4261 atatgtcgat gctctgcata ctggtacatg ggtagcagtg actggcttag cgtcattgat
     4321 gtcatcaaac atcatgcatc caacagaaga acaaatcatt gcactgatca aacggacaat
     4381 tgactcgatt ttagcattag ataatcctga aataatcaga tagtgttcta tcagtatgaa
     4441 tttgatcggt aagtccgttg gctgcaaact gtcaatggac ttttttgcta aaaaaagcga
     4501 tttctgttat ttatctatta gaaataggct tgacatttcg tgaaaaaagt ttaaaaatag
     4561 tacttacgga aggttggcag agtggtaatg caccggactc gaaatccggc gaaccggcta
     4621 ataccggcgc gcaggttcaa atcctgtacc ttccttatat caacgattag aggttgtgaa
     4681 gaagctgtcc tgcatcaagt aataagaacg gccaaacaaa aatagctttt cctatttttt
     4741 cgtttgattg ggcttattac cgcagatgca agcttttgaa caccgtttgt tcggaataag
     4801 gggctgtgac aagacttttg tcacagcccc ttattgttgt acttttaacc tacaatattt
     4861 tttgattccc cctgagtact attttttata aatgctagta aatcaatctt ctttggcctt
     4921 tttttgataa aaaaaatact agatgtaaga taaaagtgta aaaacgattt gttttaggac
     4981 gggaaacgat gtgtaccgca atcacttatg caacgaaaga ccattatttt ggaaggaatt
     5041 ttgattatga gatgtcttat aatgaagtgg tcactattac tccaagaaat tatcgtttcg
     5101 attttagaaa ggtaaaaaat ttagataaac attatgcaat gataggaata gcagctgggg
     5161 tatccaacta tccactctat tatgaggcta caaatgaaaa agggttgagt atggcaggac
     5221 tgaattttcc tggcaatgct gattataaag aattacaaga aggaaaagat aacgttgcac
     5281 catttgagtt tattccatgg atactagggc aatgctcaac catagacgaa gctaaagaat
     5341 tattggctac tatcaatcta gtcaatatcg attttagtga aaaactaccc ttgtcacctt
     5401 tacactggtt attagctgat aaagaaaaat cgatagtaat tgaaagtatg aaagatggac
     5461 ttcatttata tgataatcct gtcggtgtgt tgacaaataa tccgccattt gattatcaat
     5521 tatttaatct gaacaattat cgctcgctat caaatggaac accagaaaat catttttcaa
     5581 accagatcag cttgaatgtt tatagtcgtg ggatgggagg attgggctta cctggagatc
     5641 tctcttctgt ttctcgattt gtaaaagcca catttactaa aatgaatgct gcatcaggtg
     5701 attcagaatc tgaaagtatc agtcaatttt ttcatatact aggctcagtc gagcaacaaa
     5761 aaggagtatg cgatactgga gaaagaaaat acgaatatac gatttattct tcttgctgta
     5821 acgttgataa agggatttat tattatcgta cgtatgaaga cagtcaaatt actgctgtaa
     5881 atatgaataa agaagacttg gatcgtcatg aattggtcag ttatccagtc ataaaagagc
     5941 aacagataaa ctatgtcaat taattaatga atagatccgt ttggtatcat attttctgat
     6001 acagtcataa ttagacacga tctttaggtc gtgtcttttt ttgctggttt ttttggaaat
     6061 agcatccact aatatttttt taacacgaag agaataaggt agcctcaaaa aagaaaacat
     6121 ggtaaattag atattgggac tttattttct gtaaaaacag aaactttcat cttttgtttt
     6181 cacgtgacga aattgtaaga gagaaaatat tcgtccgcta tttattctat gataagatgc
     6241 atatgaatga aataaacagt aattttatat taggatgtga aagttaaatg tgcggtattg
     6301 taggttttgt taacgacaag gacaacaaaa aaacaattat taatacaatg atggatcgaa
     6361 tcgttcaccg tggacctaat agttcaggag aatatatcga taaacatgtt gctttaggat
     6421 tcagaagatt aagtattatt gacttagagg gcggtactca gcctatttac aatgaagatc
     6481 ggacaaagat cattattttt aatggcgaaa tctataatta ccagcctttg agagaagaat
     6541 tgattgcagc tggccacgtt tttcaaacac atgcagatac agaagtcctt ttgcatggtt
     6601 acgaagaatg gggaacagaa ttattgcaaa agatccgcgg aatgtttgct tttgctattt
     6661 gggataatga aaagaatgag ttattcggtg caagagacca ctttgggatc aaaccatatt
     6721 attatgcaga aatgaacgga acatttatgt ttggttcaga aatcaaaagt tttctgcctc
     6781 atcctgattt taacaaagaa ttgaacaaag aagcgttgaa accttatatg actttccaat
     6841 attctccatt aaacggcgaa acattcttca aagatgtcta tcgtttgcct gaaggccatt
     6901 attatacgta taaagatgga aaaatagaca ttcagcaata ttgggatgca gattttgaaa
     6961 caaaagaaac gcattcccga caagaatgga tcgaaaaaat cgacgaaacc gttcaagctt
     7021 ctattgaagc acatacggtc agtgatgtag aagttggttc tttcttatcc agcggggttg
     7081 attccagtta tgtaacctct gtattgaaac cagatcattc tttctcaatc gggtttgatg
     7141 ataaaaccta taatgaagcg attgaagcca gaaaactaac cgaactattg gacttagata
     7201 atacagcagc agtgatcgac ggagacatgt cgttcaaagc attccctttg atccagtatc
     7261 acttagatga acctgattca aatccttctt gcgtaccttt gtacttttta gcaaatcttg
     7321 cttcacaaag tgtgcgggta gtccaatctg gagaaggggc ggatgaactg tttgcgggat
     7381 accaaacgta tggattccat acgaattcaa aattcatccg tgtgattgct caaggattga
     7441 aaaaattacc aaaaggaaca cgctataatt taggacgtaa aatcggaaaa atgaaaaatt
     7501 tccatggacg gatccattta tatgaatcac ttgctcctgc aaaagaatat tttatcggtc
     7561 atgctcgagt gttcgaagaa tcagaagcat cagaagtgtt aacaccaaaa tatcaaacag
     7621 caccatctgt ggatgaaatc atgactgttc attacgagaa aacagaagga atcaaagatg
     7681 aagtcaataa gatgcaatat gtcgatcttc atcaatggat gccaaaagat atcttgttaa
     7741 aggcagataa actttctatg gcaagttctt tggaagtccg agtaccttta ttggatatcg
     7801 aagtcatgaa attggctcaa caaataccaa gtaagttttt attgaatcaa aacaacacaa
     7861 aagatatttt cagacaagct gcgaacaaac atcttccgga aaaatggtcg aatcgtgtga
     7921 aattaggatt ccctgtgcca atcaaagctt ggttgaaaga agaacacggc tacgaacaag
     7981 tcaaagcatt gttcgaagca gattttgcaa aagaattttt tgatcaagaa aaaatcatga
     8041 agttgctaga tgaccatcac gaaggacgta aagaagagca aagaaaaatc tggacaatct
     8101 tcagtttctt gacatggtat aaagtctact ttgtagatga aacgattccg caagcagaag
     8161 caatcgacta tgtgactgtt tgatgaaaat caaatatcaa taaaaaagag gttttctcct
     8221 accgtttaac actgagtgat aagaaacaat ttcaaaaaac agctcttccc gttttataga
     8281 tattatgact tatggttcgg agttggcaga ggaacaccat tgtttcgaac ataagggggt
     8341 tgtgacacga ttttcgtcac aaccccttta catatgttca ataatcgatt tctttttctt
     8401 ttgtcccatc ttttttataa atgaacaaag ttgattcttt gttttgtgct acttgctttc
     8461 ctttttcgat tgcttctttt tttgtatcaa aatggttgct tgcctttttt gcccctttag
     8521 atttcacgat ccattgttct tcctcatatt ctacgatcac atctgaatca agcagttttc
     8581 cagcacgagg attcgtatcg tgttcatccg tcttgcttgg atttttttct ttttcgaaag
     8641 ttcgtttttc tgattcacta gcatcttcat accatttttt tgcttgatca atggcaattg
     8701 ggatcgcacg gttatcaggg tacccttcat ctaataatgc atttgcgatg tcgattgctt
     8761 tttttcttgt caatttatct aagtttttca ttgatgcagg aaagtccttc atgttccatg
     8821 gcatattagt atcccccttt attaaaaaac ataatctata taaacaagtg taagggtatt
     8881 tatacaaaaa gaaaaagatt ttgtttgaga agtaaaaccg ttgtatttat cgtcaagtag
     8941 ttttacttaa caaaatcgtg aaaaacacta gcaatctaga aaaaatcatg gtaagataat
     9001 caagtctgag agtcatctta gaggattctt gtaaaaatgg gagggaaaaa catgcgcgta
     9061 aacatcactt tagagtgtac ttcttgtaaa gaacgtaact atctaacaag caaaaacaaa
     9121 cgtaacaatc ctgatcgttt ggaaaaacaa aaatattgcc cacgcgaacg caaagttact
     9181 ttgcaccgtg aaacaaaata atgtttttaa agttctcaat tccttgctgg tcaaagaatt
     9241 gagacttttt ttatctcaaa acatctaaaa tcataaggag cggataatat gtttatttcc
     9301 atgtgggcac aagataaaaa tggtctgatc ggcaaaaatg gtttgttacc atggcgattg
     9361 ccaaatgata tgcgtttctt tcgcgaacat acaatggatc gaattttagt gatgggaaga
     9421 aagacctatg aaggaatggg ggatctttca cttccttatc gccatatcat cgtgttgaca
     9481 acgcagccaa attttcgcac gaaagaaaat gcggaagtaa tgcattcgat cgatgaactt
     9541 ttgtcgtata gtcaaacgat ctcagaagat atctatgttt cgggtggtag tcggattttt
     9601 caagaacttc ttccttacac tggtcggatc tggcgaacat tgatcgatag cacgtttgaa
     9661 ggcgatacgt atattgggaa tattgatttt tcagacttcg cccttataga ggagtatgag
     9721 gggataacgg atcaagaaaa tctatatgcc catcgttttc aaaaatggga aagagtaaaa
     9781 aatagggttg cttcaaaaga agaataaaat ctatgcgtct agtttgttaa taagaaaaaa
     9841 agcatatcac gactaatcgt gaagatgctt tttttagtta aaatcaattt agtttttctt
     9901 tgatatcatt gaccatttcc tgactggcaa taggtccgtt atatagccaa ctagattcag
     9961 gtccgaattg cagtacatga ttgtctgtca ctgctttaag attcttccag cttggttcat
    10021 tgaacatggc agcatctttg tcactgttca caaggatgat gtagtcagca tctaattcag
    10081 ctagcttttc agaggaaaca gcagaccagt cagaagtggc ttttttggag acctcttcca
    10141 ctagatcagg aacaccaaat tccagatcgt cgtatacgat tcttccactt gaccgatttt
    10201 tgctgaccat aaaggctgag ttgttggtca cccacaacac tgcagctgtc ttgttctcga
    10261 ttttatctgc tagttcttct cgagttttct tgatggattt ttgataatcg gaaatgatct
    10321 tttctgcctc tttttctttt gccaaagctt tgccgacatc ttttagttgt tcttcccatg
    10381 tgacatctgt tccgtttttt acaacataag ttggcgcgat tttgctatat tgttcgtatt
    10441 tcccgccttc gacagtagcg gaagaagaaa tcaacagcaa atcaggctca aagcttaaca
    10501 ctttctcata aggaaggtca taagaaatcg ttgggacatc ctttaaatca tcttgcagat
    10561 aatcttgaat ggaaccactg ccaacagtcc actgggcgat aggtttttcc cctagtgcaa
    10621 tcagatagtc ttctaagtaa ctgccgatga tccgtttggg cgcgtttgga atctctactt
    10681 tatgtccaag tgtatctgtg actgtatgag tactattgga tgaagtcgtc gaattggcag
    10741 ttgtagctgt ttctttatct gaagaggggg agcaagcgct cagtactcca gccagcagca
    10801 atatgctaga taatgtgaat acttttgttt ttaatttcat ttctttcctc ctaacataat
    10861 tgagaatctt tctcattggt tattattcct gatatttcga acgctgtcaa ccgcttcatt
    10921 tggctataga tatgaaagta gcgcttgacg aaatgttgaa acagcgtcat aattgagaat
    10981 gattttcagc gaaaggatgg gtgaaatgac gagattaaga gcagctgaca ttgcagccgg
    11041 ctacgaaaac aaaaaaatca ttgaaaacat gtctgttacg attcctgaag ggaaaatcac
    11101 tagtttgatt gggccaaatg gaagtggaaa atctacttta ttgaaaagtt ttacccgtat
    11161 actacctgtg aaatcaggaa agatcattgt caataactct gatatctcta catacagttc
    11221 aaaagaactt gcaaaaaaaa ttgcattatt agcacaaact agtgaacacc ctacaggtat
    11281 gacagtagaa gaagtagtat cttacggacg atacccttat caaaaactgt tcagtggcat
    11341 ggacacgcaa gatcatgaag caatcgagtg ggcgttggat gctacaggtc tagctggatt
    11401 tcgtatgcgg gatatcgcaa cgctgtctgg tggacaaacc cagcgtgtct ggatcgctat
    11461 ggcgcttgct caggaaacag atatcttgat cttggatgaa ccgacaacat ttttagatcc
    11521 ggcacaccaa ttagagatcc tacagttatt gactgaaatc aatcaaatga aacaaacaac
    11581 gatcttgatg tcgattcatg acattaatca tgcttcacgt ttttcagatt atttattagc
    11641 aatgaaaaac ggtaagttga tcacagaagg tacacctgaa gaagtgatca caaaagagtg
    11701 tatgaaaaaa atatttgaga ttgatgcaac gattgtcacg cttccaagaa caaataaacc
    11761 agtttttcta acctatgatt tgttgccaaa agagcaggag gatcaattat gaggactatg
    11821 aagaaaaata gactcctgct tttggtctgt tttgttcttt tgattatttt ttttgttgct
    11881 ctttcaaatg gcgcagcgac tgtgggaaaa gcagattttt ggcaggcgtt gtttgatttt
    11941 gatagatcaa aacaaagtca ccaaatcata cgaaatattc gtttgcctcg tgtggcggga
    12001 gcatttcttg tagggagttg ttttgcattg agcggtgcat tgatgcaagg agtgacaaga
    12061 aatcctttag cagattcagg cttactagga atcaacgcgg gtgcttcgtt ggcaatggcc
    12121 cttagttttg ctttttggcc taaagcttct gatttttctg tgtttctaat gtcattggca
    12181 ggttcttttg ctgtaacttt gctcgtattt ggttttttgc agttttcgaa aatgggcatg
    12241 aatcctttac agctgatttt agctggcgta gctattagtt ctttttttac tgctatcagc
    12301 caagcactta cccaattctt taatctgcag caggatcttg ctttttggtt tgtaggcgga
    12361 gctgccaata tcacatggaa acaattagca gtattgtttc ctttatatat acttgctgtt
    12421 ttggtttctt tttcattagg aaaagcaata tctttgattc atttgagtga aacacatgca
    12481 ttagctcttg gaagtcatcc taaacgaacg cggttgattg ttttctgtct agttgcagtg
    12541 ttagcagcca ttgccgtttc gttagtcgga cctgtttcat ttattgggct gttggttccg
    12601 ccagttgttc gcggaatggt tggaacggac tatcgttatg ttctacctgt ctctttttta
    12661 gttggcggtg gattggttat gttggcggat tttacagcga ggatgatcaa tccccctttt
    12721 gaaacacctt ttggattagt cattgcattg attggcgttc cgtttttact gatacaagta
    12781 aggagggaac aacggtgaaa aaaatatatt ggacgttcta tttcttgctt tttgtacttg
    12841 tcatcctgtc tataggtagt ttaatgattg gtacgccttt tatttacctg gatcagcttt
    12901 ttgccacgtt catagggaaa gaatcatctt ctttggcgct gattgttaga gaatttcgag
    12961 ttcctcgatt actgatcagt ttattagcag gagcttgtct tggcgtttct ggttatatct
    13021 tgcaaggagt aacaagaaat gaattggcag attctagtat attgggcatt aatgccggag
    13081 caggattttt agtgatgctt tatttaggtt tcttttcgca ctattcgctg ccgtttctat
    13141 tgctagttgt agcctttcta ggtggtgttt tagctgcttg ctgtgtctat cttgcggcct
    13201 atgatcgaaa tggctttttg ggtatgaatc gaatcttatt gtccggtatt gccgttaatg
    13261 ccggcctttc tgcattaaca ttgctatgca cgatccaatt atctaaggaa aattatggat
    13321 ttgtgaatgc atggttagcg ggatcaatct ggggtgcgag ctggccttat gtttgggcat
    13381 tgcttccatg ggcggttatc ttaattccgc taggaggact ttatgcacag aggattgtat
    13441 tgctatcatt tggtcaagaa cgcgcgcaag gattaggcct taacgtggct aaaagtcaaa
    13501 aaatcctttt gttgttagct gttgctttgg caagcggtag tgtagcggtg acaggtagtt
    13561 tatcttttgt aggactacta gctcctcatg ctgcgaagct agtcgtacgt aaagaaaata
    13621 actggacctt tgttctgagc ggtttgttcg gttccatttt tctgcttagt gcagatatgg
    13681 ttgctagagt gattctacta agtggggaag tacctacagg aattttgatt gccctttttg
    13741 gtgcgccata ttttctgtat gcacttttta tcagacaaaa acacgcgctg aacagttgaa
    13801 attcattaaa aaaggaaggc caatcatttc taggtcttct ttttattcta tttatgcatt
    13861 tatttgaaag ttcgattgct atttttcttt ttttcctata ccattttatg agagaaggaa
    13921 gggtgagttt ttggaccaat tgaaagtaag attacgccgg aaaggaatcg aaaagctgaa
    13981 aaatctggcc aaaagtggca agaaaaagga aaaagaacag cgaatctata aacagctatt
    14041 cgccagcgag tactggaagt cagcaaaagt gatagggacg actttatcga atgaatttga
    14101 actggataca cagccggtta tccaacgagc agaaaaagaa caaaagaaaa tagttgtacc
    14161 taaaactttg ccgaaacgtc agatggcgtt ttatgaaatg gatgaacagt ccgttcttgt
    14221 acgttcgagt tttggtgttc ttgagccaca atcagagaag ctctatttgc cggagatgat
    14281 cgatctcctc attgtaccag gtattatcta caatagagcc ggttatcgaa tcggttttgg
    14341 cggtggttat tacgaccggt atttaaataa ttttcataaa aaaacatgta gccttgtttt
    14401 tcgtgaacaa ttgaatgaac aatggcaacc ggagagcttt gatcagaaga tccaacaatt
    14461 gtttattgac taaaatgaaa tgggaggttt tcatgaatta tcaacagcaa ataaaattgc
    14521 gtgcatggtt aagacgaccg ttatggacct atgctttttt agggatacag acagcggtct
    14581 tcctcatgat ggaattattt ccgcgacttg agatccctta ttatgccgga atgtatggtc
    14641 cttatcttgt tcacttcaat gaatggtggc gtttagtgac accaatcttc atccattttg
    14701 gactgatgca tttcgtaatg aactcactga tactgtattt tatggggcag caaatcgagg
    14761 caatctatgg ccattggcgt tttttcttga tttatatgtt cagcgggata atgggaaata
    14821 cagcaagctt tgcttttaac gaagcgaatg ttctgtctgg tggtgcaagt acttcgatat
    14881 tcggtttgtt cggtgcgttg tttatcttag ggtttcattt taaacataac ccagcgatcc
    14941 agcagctagt cagacatttt cttttattta ttgtgttgac atttgttttt ggtcttctgg
    15001 atacctctgt agatgtttgg ggacatgtcg gtgggctaat tggcggatta gtgttaggga
    15061 atgttttggg tctaccgaaa caacagacaa gctattcgat acaccaaagg attctttcta
    15121 cactagtttt tgtatttctt tttgtaatat gtattttact gggtttaaaa aaatatggtt
    15181 tacttgtata atatgaagga agtgcttaaa gatggagagc ttatatgatg tacagcagct
    15241 gttcaaaaaa tttggcatct ttatctatgt gggtgcacgc atctatgaca ttgaattaat
    15301 gactatcgaa ttgcgtaatc tgtatgatag ccgtttgatt gatcatgata catacatgac
    15361 agcgtggcgc atattaaaaa gagagcaccg aattgaagaa agccgaaaga aaggaaattt
    15421 gtaatggaca agaagattat tgggattgat ttaggtggaa caacagcaaa atttgcaatt
    15481 ttaacaccag aaggagagat tcagcaaaaa tggagcatcg ataccaatat tttggatgat
    15541 ggtaagcata tcgttcctga aatcattgaa tcaatcaatc atcgtttgaa tttatataat
    15601 atgaaagccg aagactttat cggaatcggc atgggtaccc caggaagcgt ggacagcgaa
    15661 gctggaacgg ttattggtgc gtataactta aactggacag aagttcaatt tgtgaaaaaa
    15721 ctgatcgagg caggaacagg aatcaaattc gctatcgata atgacgcgaa tgttgctgct
    15781 ttaggtgaac gatggaaagg tgctggagaa aacgatccag acgttgtatt cgttactcta
    15841 ggaacaggtg ttggcggagg tatcgttgct ggcggaaatt tgattcacgg cgtgagcgga
    15901 gctggaggag aaatcggaca tatcactgtg gatcctgaag ggttcgaatg tacttgcggt
    15961 aaacgcggat gtttagaaac tgtatctagc gctacaggtg tggtacgcct aggtcgtttc
    16021 ttggcagaag aatacgcagg ggattcaaaa ctgaaagcaa tgctagacaa cggagaagaa
    16081 gtaacaagta aagatatctt tgaaatggct caagaagacg atccgtttgc attgatggtt
    16141 gttgatcgcg tatgcttcta cttaggttta gcttgcggaa acttggggaa tacattaaat
    16201 ccgtcaagta tcgtgttagg tggaggagta tcggcagcag gagaattttt acgtagccgt
    16261 gtagagaaat attttaaaga atttactttc ccgcaagtaa gagaatcaac aaaaatcaaa
    16321 ttagcagagc ttggtaatga agctggtgtc attggtgctg cctcattagc cttgaaattt
    16381 gcttaaaaaa acggaggcat tacatggttt tatgggtaat taatatcatt ttgctattga
    16441 ttgtgcttgc aatcatcctt tgggaagtct atctacgagt aatggccaaa cgttcagcaa
    16501 cgactttgac agaagaagaa tttcgagaag gaatgagaaa agcacaagta atcgatgttc
    16561 gcgaaaaaga tgtgtttgat gctgggcata ttttaggtgc aagaaacatc ccttacactg
    16621 ttttgaaaga ttcattagga tctattcgaa aagaccagcc tgtctatatt tacgatcaaa
    16681 agaaaagttt gagcatccgt acagcaaata aattacgtaa agcaggttat caagagattt
    16741 atttacttaa gggtggatat gacggatggt ctggaaaaat caaaagtaaa aaagtataaa
    16801 acagatgatt gtatgacagc tttaaaaacc gaatcaatca ttagattttg accaagatca
    16861 catgaacggc gcaatggata gttgtgccgt tcattttttt tattttgtgc tattatctac
    16921 atatataagc taagcgaaaa ggtagaaaga aggaaacaac tatgatcaaa ttaaaacgtg
    16981 tttaccttga tgctgaaaaa gaggatggct atagaatact cgtggataga ttctggccac
    17041 gaggaatttc aaaagaaaaa gcgcagttag atctgtggtt gaaagaaatt gcacctagta
    17101 atgaagcacg caaaactttt gaccatgtac cagaacgatt tgcttcattt gcaaagaaat
    17161 accagcaaga attgattttc gaagaagaaa agaagcaagc tatttttaaa ctcagtcaac
    17221 ttatgaaaga acatccagtc attacttttg tttatggtgc aaaaaatgaa catcagaacc
    17281 atgctgtttt attgaaaaaa tttatcgaaa tggaggcatg caaatgaaaa tagggatcat
    17341 tggtgccgga cctagaggat taagtgtagc agaaaggatt ctccataata atcaggacag
    17401 acattctctt gaactttttc ttttcgatcc caatggtcct ggaggtcgag tttggcggtt
    17461 gaatcagcca ccggagttat taatgaattc tgtttctcaa caagtgacgc tttttacaga
    17521 tgaaacattg tccagtggag gattggtaac acagggaccc aacttgtttc aatggagtaa
    17581 aggacctgct acagattata tcgaacagac ggtaaccaaa aataagcaac taattataga
    17641 agaaatcaat cgtctggaaa aagatcagca atctactcga tgtttatacg gactctatca
    17701 acgctggttt ttctcaaagt tgcaagaaga atttccagct tctattcagt ttgtttattc
    17761 tttagtaaaa aaagtaagca aaagaaaaga tggttatctt ttacagacag atcagcaaag
    17821 ctattctatc gatcagcttg ttttagcaag cggtcactgg gaaaatgatg agacagaaga
    17881 agaaagagtg tgggtagact acgcaaagaa aaatgtttta ctgtatcagc ttccttctaa
    17941 tcctgcagat gtagatcttt cgaagatacc gcctaaagaa aatgtctttt taagaggtct
    18001 aggcttaagt ttttttgatt atgtcggatt gtttacagta tcaagaggag ggcgcttcga
    18061 gaacgaaaag ggaaaactgg tctatcatcc atcaggcaag gaaccgattg tatacagtgg
    18121 atcaagaagg gggctccctt attaccctcg aggaagaaat caaaagcaag gcggagcaat
    18181 ggcatggcca cggttactaa caaatgaaaa cctggagcaa tggcatcagt cgggattttt
    18241 gactggcgaa acattttttg actatctgaa aaaagacgtt gagttgtttt attacaagaa
    18301 aatcatcgaa gaatatcact tgccgattcc aaagaaaaat tttgagcatg attttctctc
    18361 cctctcttca gaggagtgtc tcggaaagca tccagcacta atcccttata gatgggattg
    18421 gtcgtattta gaaactcctc ttgcctatca gaatgagccc ttttcagaag ctagccgaat
    18481 atttattgaa gggcagataa aagaagcgga aaaaggcaat tgcacaggag catttgcttc
    18541 aacatttgat gcattaaaag actggcgtga tccaatccgg caggctattg aatggcacgt
    18601 tttttctgct aaggaataca aagaaatact atggggatgg tttactcatc taaatagctt
    18661 tttaacgatc ggtccgccaa tcattcggac gaaagaatta gcagcactga tagatgcggg
    18721 tatttttcat ttgatcgaac cgccatttga tattcagcaa aagcacaacc gctatgttat
    18781 ttctctacaa gataaagaaa tcagcagtcg ctatttgatt gaagctcgat tgccacaaac
    18841 aagcttttct cgttcaagta atcctgtcat aaaaaatttg aaggaaaaca aacttgttcg
    18901 ccctttttca ttttcagata atacaggaag ttatcattca gaagctatag atgttgagcg
    18961 aggtacgaat cgcgtgattg atgcttcagg ggaaatccag tctgactttt attgctatgg
    19021 aatacctgtt gaaggtgtgg attggctaac tgcagcagta gctagacctt atacagatcc
    19081 atggaatctg agacaggcag ataaaatagc acaacttata ttgactgaca aataaaaata
    19141 cgcatgatga cttatctgtt ttttagaaag tcttcatgcg tatttttaaa taattattaa
    19201 cgagaaacgc gtttacgcat tgctttttta cggttttctt cgatcattgc ttctttttcg
    19261 tcaattggat cgatcactgt ttttttcaca gaagcaacca aaccagcaac ggcagcggct
    19321 gttgccagac ttcctactaa tattccagat acaaattttt tcattgaatc cacctcattt
    19381 attatttgtt atatctatta tggaacaaaa gagaaaaaaa ggctagtttt acgtttgttt
    19441 gtaaaccttt gcaaaaaatc cttgaaaatg tttactaaat gttttattat aaatttacca
    19501 tatgaattta cgaaatttta ctttgtttgt tttcaccata aaagtaaact tcgtttttta
    19561 aattatatag aatcaacaaa cacaatttta ctaatcaaag gagtgttttt tatgacaatt
    19621 ttagtactag gcggagccgg ttatatcggt tcgcacgctg tagaccagct agttcaaaag
    19681 gggtatcaag tagctgtggt agataatttg ctcacaggtc acaaacaagc agtacatcca
    19741 gatgctcatt tttatgaagg agacatccgg gataaagaat ttctacgttc cgtatttgaa
    19801 aaggaaccga ttgaaggagt aatccatttt gctgcaagct ctttagtagg agaatctgtc
    19861 gaaaaaccat tgatgtattt caacaacaac gtatacggta tgcaaatctt acttgaagtc
    19921 atgcacgagt tcaacgtgaa taaaatcgta ttctcttcta ctgcagcaac ttatggagag
    19981 ccgaaagagt ctccaatcac agaagataca ccagctaatc ctaaaaatcc gtatggtgaa
    20041 agtaaattga tgatggaaaa aatgatgaag tggtgtgacc aagcatatgg catgcgctat
    20101 gtcgctttgc gctacttcaa tgtagccgga gcaaaagcag atgcatctat cggagaagat
    20161 catacaccag aaacgcatct tgtcccaatc attttacaag tagctcttgg tcaaagaaaa
    20221 gccttggctg tttatggaga cgattatgat acaccagatg gaacatgtat cagagattat
    20281 gttcaagtag aagatttgat tgctgcacat attttagctt tggaatactt gaaagaagga
    20341 aatgaaagca acttcttcaa tttaggcagc agcaaaggat actcagtcaa ggaaatgcta
    20401 gaagcagcta gagaagtgac aggaaaagaa attccagcag aaatagcacc aagaagagct
    20461 ggagatccta gccgattagt cgcttcaagt gaaaaagcga gagagattct gggctggaaa
    20521 cctgaataca cagatatcaa agcgattatc aaaacagcat gggattggca tgtcagccat
    20581 ccaaatggtt atgaagaata ggggaatgag atatgtcaat cagtcaaacg atttgtgatt
    20641 ttgccacact tgcgatcgaa gctggcggat ggatggaaat ggaccgctta tatttacaga
    20701 atcgtctttt agggatggta ggagaagatg ctctagaaag cattgaagta agaccagtta
    20761 aggaagaatc tgtagactta ttggatcagc ttgtcgctaa ggcaagagaa aatggtgttc
    20821 tttcagaatt gccagcagaa caagagattt ttgaagcgca attaatggat tttctcacac
    20881 cgccaccttc agtcgtcaat gcattctttg cacagcacta ttcgaaaagc ccagaggaag
    20941 caactgagta tttctttaat ttaagtaaga aaaatgacta catcaaaaca agagccatcg
    21001 caaaaaacat caaatttccg gcagagtcgg agtatggtga actggaaatc acaatcaatt
    21061 tatctaagcc agaaaaagat ccaaaacaaa tcgcagcaga acgcgaggcg gtaaaagtag
    21121 actatcctac ctgcatgctt tgcatggaaa acgaaggata tagaggtcgc ttgaactatc
    21181 cagcccgcac caatcaccgg attattcgaa tgaatctaga cggtgaaagc tggggattcc
    21241 aatattcccc ttatgcttat tacaatgaac attcgatcat tttatcagaa caacaccgcc
    21301 cgatgaaaat cacaaaggaa acatttgcta gactgcttcg gattactgaa gtcttgcctc
    21361 attactttgt tggttcgaat gctgacttac caattgttgg aggatcgatt ctttctcatg
    21421 atcattatca ggcaggacgt catgaatttc ctatggcaaa agcaccgata gaaaaactta
    21481 tcaaactaga agattatccg caagtcatcg caggagtcgt gaaatggccg atgtctgtga
    21541 tccgtttgca atcaacagag aaaaacttgc ttattgaagc agctaactca gtattggaaa
    21601 aatggaaagc atattcggat gaaacagtat ctatcagagc tttttcagca gatggtacac
    21661 cacaccatac gattacgcca attgcacgaa gaagggacaa ttactttgag ttggaccttg
    21721 tccttcgaga caacaatgtt tcagaagaac atcctgatgg gatttttcat ccacataaag
    21781 atgtccaaca tatcaaaaag gagaacattg ggttgatcga agtaatgggg cttgctgttt
    21841 taccacctcg tttgaaacca gaactagtag aagtagagca tttcttatta ggagaagaaa
    21901 accagatggc tgaatatcat cgtccgtggg cagaacaatt aaaaaaagaa catcctgatt
    21961 tgacgaaaga tacggtcacc gatgtcgtac aaaaatctgt aggtcaggtt tttgcacgtg
    22021 tactggaaga cgctggagta ttcaagcgag atgaaaaagg acaggcagcg tttgaacgat
    22081 ttactgcgat tttataaacg taaaaaagga ggagagacaa atggcaacaa ttaaagacat
    22141 tgcgcagttg gcaggagttt ctcctgctac tgtttcaaga gttctcaact atgatcctga
    22201 tctttccgtt ggcttagcca caaagcaaaa aatttttgaa gcagcagaac gcttaaatta
    22261 tacgaaacat cgtaaaaatg cagcaaatac taagcaaacg cttcgcctga tccagtggta
    22321 cgacgatacg gaagagcttg aagatcttta ttatttatct attcgcctag gaatcgaaaa
    22381 aaaggcagaa gaagcaaacg ttactttatt gaaagaaacg tgggattcca ttagtgatct
    22441 tccggcagat ggaacgatcg cactaggaaa attcgacgaa gctcagttat tagaaatgaa
    22501 aggatcccaa aatgccttgc ttttggtaga ttctgacggc acttctcaag gaatcgactc
    22561 tctagttgtc gactttaagt caagtgttca aaaagtcatt gaacactttg tctcccataa
    22621 agcgcaacat atcgctatgc ttgctggaac agaatatacg aagaaaaacc atcagcggct
    22681 aaaagatccc agaattatgg catttcaaca agttttggca gaaaaaggct acgacgaagg
    22741 tccaattatc gaagcagatt tttctgtcga gtcaggatat caagcagtga agcagtattt
    22801 aaaaacgaat ccgcatgttc ctgatgcgct ttttgcagct aatgatgcat tagcgattgg
    22861 cgcactgaaa gcgattcaag aaaaaggttt agtggtacca gaagatattt ctgtgatggg
    22921 cttcaatgat gtcagtgtag cgaaatatgt aacgccctcg ctttctactg taaaagttta
    22981 taccgaatgg atgggagagc tagcagtgga gacgattctt caattaagta attccaaagc
    23041 accggtttca agaaagatta ttgtggaaac agagttgatt attcgagaat caacgaaata
    23101 aaagatggaa aagctgtggg aaaatggagt gataccgcag cttttttgtg tttcaggaag
    23161 gggcaaaatt tgcccgccag cagataatca tgtattattg tactagtttt gcctttggta
    23221 ttttcatcct cagaataaaa aagtatgcta caatggaagg cgagaatgtc atagcagaaa
    23281 ggatcaaata tatgattaca ttaggaatag atactgcaaa ccaaactttg gcgatcggtg
    23341 tggtagaaga cgaacaaatt cttggacaaa tacaaacaaa tatcaaacgc aaccacagtg
    23401 taacactgat gcctgcaatc gaccaattgt ttgcagatct tcaaataagt ccaaaagaca
    23461 ttgaccgaat cgcggtttct gatggtcccg gttcttatac agggcttcgt ataggtgtaa
    23521 cgacagcaaa aacgattgct tatacattag ataaagagct agtaggagta tctagtctga
    23581 aaaccatcgc ggctaattgc atcggtgtgg ataatattat tgtgcccatg tttgatgctc
    23641 gaagaaaaaa tgtatacgct ggggcatacc gctggaaaga aaatagatta gaaactgtac
    23701 ttgaagatgt acatatttct gcttctgatt tgttcacaca actacaaaag ctgaatgaag
    23761 ctatttattt cgtcgggagt gattgtcaca aattcttctc tgaaataaag gaaatattgc
    23821 ctagtgcgat gattaataca gttccagcat gggatattcc aagtggtgta actgtggctc
    23881 aattaggtgc tttagttgaa ccagttgcag agaaacaagc ttttcttcct cgctatttaa
    23941 aacgagtaga agcagaagaa aaatggttgg agacgcacac acctggagac gaaagttatg
    24001 ttgaaaaaat ttagagcgta tgccagaagc ttttttcaag ataaaatgat ttatacagaa
    24061 aaaacagtcg aacaaatcga tcgtcgttac gtactaagag aattgactac agaagatatc
    24121 aaagagctgc tggatgtcga acgagaagtc tataatggcg aattgccatg gacaaaaagt
    24181 gcctttatat cagaattgaa gtcaccgctt cctcatctat atctaggtat ctttgataat
    24241 ggaagaatga ttggttttat cggttcaaga gcgttcggtt tagattgcca tatcacgaat
    24301 attgccgttc gaccggcata tcagcgtaaa cgaatcggtt cgctgcttat tgacgaaatc
    24361 gagaactttg ctataatgaa tcgctgtgag acgatgtcat tagaagtgcg tatgagtaat
    24421 caagatgcac aaagacttta tcggaaattg gggtttcaag caacaacgat caaaaaaaga
    24481 tattacactg aaacaaaaga agatgcgttg gatatgataa aacttttaga aaatgagtga
    24541 gattagttga tttgcagaaa aaaagatttt gatgaagcgt acttggcaga atgcgtatgg
    24601 atgattagcg aaaatgctta tccacatggt tcaccttgga cgaaaatgca gtttttggca
    24661 gacatccaac agcctcactc tgattatctg cttttagctg aagataacca gatctgtggc
    24721 tttattggat actcgaaagt aatagatgaa gtcgagatca caaatattgc tgttgcagtt
    24781 tcagaacaag gaaaagggca tgcacggcat ttgttacaat tactgattac ggaacaaata
    24841 aaagattcgc tgcatgtttt tttagaagta cgtctatcta atgaagcagc aagaaaactt
    24901 tatgaatcag aaaagtttcg tatattaggt aaacggaaat catattatcg caatccgacg
    24961 gaagatgcga tcattatgag cagaaagtta aaaacggaga cgatgaaatg aatgaagaat
    25021 tgattctggc gattgaatca agttgtgatg aaacgagtgt agcggtcgtt agaaatggaa
    25081 cagagatctt atctaacatc gttgcttctc aaataaacag tcataaacgt tttggtggcg
    25141 tagtacctga agtagctagc cgccatcacg tagaacaaat caccttatgc ttggaagatg
    25201 cactggtaga agctggtgtg tcggcagaag atttatcggc agtcgctgtc acttatggac
    25261 caggacttgt cggctcgttg ctgattggta tttctgcagc taaggctttt gcttgggcac
    25321 accaattgcc gcttattcct gtaaaccata tggcagggca tatctatgca gcccgtctgg
    25381 ttaaaccatt ccagtttcca ttgatggcac ttcttgttag cggaggacat acagaattag
    25441 tctatatgca agaagatgga tcatatgaaa tcattgggga gacaagagat gacgcagcag
    25501 gagaagctta tgacaaagtt ggaagagtat taggtctttc ttacccaagc ggaaaagaaa
    25561 tcgatcagct agctcatcag ggaaaagata attaccattt tccgcgagcg atgatccatg
    25621 aagataatta tgatttcagt tttagcggat taaaaagtgc atttatcaat ttggtgcata
    25681 atgcacaaca gcgtggagaa gatctagaca aaaatgattt agcagctagc tttcaagcaa
    25741 gtgtgatcga tgtattgatt actaaaacac ttcgagcttg tcaaaattat ccagtcaagc
    25801 aactgattat agctggaggt gtagcggcca atcaagggtt gcgtgaagga ttgcaggatg
    25861 ctttggcaac taaactccct gaggtagaac tagtgattcc ccctcttcga ctttgtggag
    25921 ataatgctgc aatgattgga gcagcagcac atgtggaaat gcaaaaaaga aattttgcta
    25981 gctatcagtt gaatgctgat ccaagcttgg ttttatctga ataaagaggt tgtgaaaaag
    26041 ctgccctgca tcaagtaata agaacagccc aggaaaaacg gcttctcatg tttttgacct
    26101 ggctgttctt attaccgcgt atgcaagctt ttgaacgccg tttaatcaag agttcgtaag
    26161 cctgtcgttt aactctgatt cacaaggaac aatttcaaaa aacagctcct catgtttatg
    26221 aacattatga cttgtgaccg aagagttgac aggtacacac cgtttaatcg aaatagggct
    26281 gtgacacgtt tttgtcaaag ccctattttt tacatacttt taatcattat tgttattttg
    26341 ctatttttta cagcttattt atcattatcc gttcctctga ttgccctcac tgagtaatcg
    26401 tttattgtcg aggaatatcg aataatctag atcggatgag gacaagacgc ttcatctagt
    26461 taatctaaca gtattttttg caaacagtga atattacata actgaaacac acatagaaaa
    26521 tctttgagtc ttgtccagtg atctaaaact gccggatctt tcagaagttt tagggaggaa
    26581 aataagcagt gaccctgata tttatcaggg tcactgctta tttttgttcg cgttattctc
    26641 tcatagctgg agtagtagct gtcttacaga aagtaaaacg ctttttgtca taatctaaca
    26701 gcccggaatc acgtagttgt ttgattactt gtccagcagt ttcacgtgtc gtgccgctat
    26761 tcactgctaa ttcttttaag gtgatcggat aaggaatggt gatatgacca tcttttgttg
    26821 gtacgcccaa ttcttctttc aaaatgaata aattttgttt tacccggtca aaggcgctag
    26881 aggtcaatcc gatttggata cgcaattcat gtatttcaac tactttagaa attttttgta
    26941 tcatatagat caactgttcg ggattttttc gtgctaattt ttcatagtag gaaacaggaa
    27001 ggtagaatgt ttcaatatca gtcatcgctt gagctgtata agtgtaggtc tcttctccaa
    27061 acattcctga cataggaaaa aggttatttg tattcacata gtcatagtag taataagttg
    27121 cactttcatc ataacgttct aaacgaacga gtcctttggt caagatatac aaccgttgtc
    27181 tgggatcacc atcgtcgaaa aggatctgtc cttttttata agaacgaaga acagtatgtt
    27241 ccttgatctg ttggaactct tcatccgaga aatgttcaaa atcaggttga tatctgagtt
    27301 ttgaaaagtg aaaatcgatt ttattaacac gcattaaaaa gtcctctcct tattgttcac
    27361 tatatttatt gtcacttttt ttaaagaatt tgtaaagttt taacatacca acattagaaa
    27421 caagttttaa tactttacaa atataatttg ttattatgta aaaaatatac aatggtattg
    27481 aataatttgt tttgaagcga atgatttgtg tataaaaaac aacaataata catatagtaa
    27541 acgctaacaa cgataatata atttattttt ttaatttatt caaaaaaatt ttttcaatga
    27601 ataaatattt ttttgaaagt gtatacagaa taaaaaatga ataaattacg cttaaaaata
    27661 gaaaaacgtt taattattta attatcgaat attaatgtga ggaatgtctc attttgaatg
    27721 agacaaaatt atgaaagtga ttacaaaagg aggatatcct atacatgtaa tcaaaaagaa
    27781 ggaggcaaca caacatggat aaacctattc acgttttctc tgaaataggg aaattaaaga
    27841 cagttttact gaaacggcct ggacaagaag tggaaaattt gacaccagat attatggatc
    27901 gtctgttgtt cgatgatatt ccatatcttc cgattgctca agaagaacat gacaactttg
    27961 cgaagacatt gcaaaatgaa ggcgtcgaga ctctttactt ggaaaaatta gcggcagaag
    28021 cgattgacgc aggcaatgtc aaagaacaat tcttgaataa aatgttggat gaatctcata
    28081 ttgcatcgaa tgctgtaaga gagggtcttc atgagttcct tctatcaatg gaaacgcaag
    28141 aaatggtaga taaaatcatg gctggggttc gtacaaaaga tatcgaagta cgttctagca
    28201 gtctatatga tctgtcagca gacgatgact atccatttta catggaccca atgccaaact
    28261 tgtactttac acgcgaccct tccgcatcaa tgggaaatgg gatgacagtc aataagatga
    28321 cttttgaagc acgtcgtcgt gaatcgatgt ttacagaata tattttgaaa catcatccaa
    28381 gatttgcaaa taaaggaatc gaagtatggt tggatcgtga aaatccagat catatcgaag
    28441 gcggagacga attgatctta agcgataaag tagtagctgt tgggatttct caacgaacta
    28501 atgcgaaagc tttagaaaaa ttagcacgcc acctattcgc aaaaaattca ggatttgaaa
    28561 aagtgttggc aatcaaaatt ccgaacaacc gtgcaatgat gcatttggat accgtattta
    28621 caatggtaga tcacgataaa ttcactatcc atccagcaat tcaaagtaaa gacggtaaga
    28681 tggatgtctt tacaatcgaa ccagatggtg atgatatcaa gattactcat tcagataact
    28741 tacacgaaac gatgaaagct gctcttggat tagatgatct tgtactgatc ccaactggta
    28801 atggagatga gatcgttgca cctcgagaac aatggaatga tggatcaaac acattggcaa
    28861 tcgctccagg tgttgtagtc acttataacc gaaattatgt atcgaacgaa ttgcttcgta
    28921 gctatggcat caaagtgtta gaaatcaatt caagtgaatt gtcacgaggt cgtggtggtc
    28981 cacgctgcat gagccaacca cttgtccgtg aagacttgaa gtaatttgtt tcctgacggc
    29041 agtcttactg tcagctgccg tcgggatttc ctctttaatc ttaaataaaa acaatctaaa
    29101 ggagaatgtt taatatgaaa gaatcagtat tccaaggaag aagcttatta gcagaaaaag
    29161 actttacaaa agaagaatta caatatttga tcgatttttc agaacacttg aaagacttga
    29221 aaaaacgtgg tatcccacat cactatctag aaggaaaaaa tattgcgctt ttatttgaaa
    29281 aaacatctac acgtactcgt tctgcattta ctactgcagc gatcgatcta ggtgcacatc
    29341 ctgaatatct aggtgcaaat gatatccaat taggtaaaaa agaatccaca gaagatacag
    29401 ctaaagtact aggacgtatg tttgacggaa tcgaattccg tggattcagt caaaaaatgg
    29461 tagaggaact agcagaattc tctggtgttc cagtttggaa tggcttgact gatgaatggc
    29521 atccaacaca aatgatcgct gactttttaa ctatccaaga aaactttgga acagtagaag
    29581 gtatcactgt tgcttactgc ggagatggtc gaaacaatat ggctaactct ttattagtca
    29641 caggtgcaat tttaggagcg aatatgcgta tcgttgcacc aaaagaatta caacctgaag
    29701 aagaaatcgt caaaatggca gaaggctttg ctgaaaaatc cggtgcacaa ttgatgatta
    29761 ccgacgatgt tgataaaggt gtagacggag cagacgtcct ttattctgat gtatgggtat
    29821 caatgggtga agaagataaa ttcgaagaac gtatcaaatt gttgaaacct taccaaatca
    29881 atatggaaat ggtagaaaaa acacacaaca cagatcgttt gatcttcttg cactgcttgc
    29941 cagctttcca tgatacaaac actgtttacg gtgaacaaat gaaagaacgc tttggcatca
    30001 ctgaaatgga agtaacagat gaagtcttcc gcagcaaata tgctcgtcaa tttgaccaag
    30061 cagaaaatcg tatgcactca atcaaagcga tcatggcagc aacattaggt aacttgttta
    30121 tccctcgcgt ttaagcataa ccacattcat ctcacgaaaa aacgagtcag cattgaggga
    30181 aaatcacaaa acgctggctc gttttgttat ctagaaaatt cggtagaact gaagaaagga
    30241 cgaatgaaat ggccaaacga aaagtagttg tcgctttagg gggcaatgcc atcttgtcta
    30301 cggatgcaag tgcaaaagcc caacaagaag cgttaatgga aacagcaaag tatttagtga
    30361 aatttatcga acaaggagat gaattgatca tctcccacgg aaatggtccg caagtcggta
    30421 acctattgat tcagcagcaa gctgctgatt ctgagaaaac accggcaatg cctttagata
    30481 catgtgttgc aatgacagaa ggatctatcg gttactggtt acaaaacgcc atgggcgaag
    30541 tactgaagga aaaagggatc gataaagatg tggtttcttt agtcactcaa gttattgtgg
    30601 atgaaaatga tccttcattc aaaaatccaa gtaaaccagt cggtccattc tacacggaag
    30661 aagaagccaa agaacaaatg aatgctgact ccactgtgac attcaaagaa gatgctggac
    30721 gtggatggag aaaagtagtc gcttccccta aacccatctc gattaaagaa gcacgtgtca
    30781 tcgaaacgct ggtcgatcaa ggagttatca cagtctctgt tggtggcggt gggattcctg
    30841 ttgtagaaac agctactgga ttagaaggac gagaagccgt aatcgataaa gattttgctt
    30901 cagaaaaatt agccgaaatc atcgatgcgg atttgctgat cgtattgaca ggagtagaca
    30961 atgtgtatgt caattaccaa aaaccagatc aaaagaaatt agaaactgtt actgtttcag
    31021 aaatgaaaca atacattgat gaaaagcaat tcgctccagg aagcatgctt ccgaaagtag
    31081 aagcagctat tgcatttgtt gaagcaaaac caaacgctaa ggcaatcatc acttctttag
    31141 aaaatatcga gaatctttta gcttctgaag aaggaacgat tatcgtagct gattaatttg
    31201 ttaagtcttt cgtatttacg aaatgattta tagctatcca ggtggtattt cttctccttt
    31261 aggccatctg gat
//
LOCUS       DACEPD010000023        31002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.023, whole genome shotgun sequence.
ACCESSION   DACEPD010000023 DACEPD010000000
VERSION     DACEPD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.023"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            163..1104
                     /locus_tag="IW797_001049"
     CDS             163..1104
                     /locus_tag="IW797_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ1223836.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1173..1904
                     /locus_tag="IW797_001050"
     CDS             1173..1904
                     /locus_tag="IW797_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ1223837.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2048..4228
                     /locus_tag="IW797_001051"
     CDS             2048..4228
                     /locus_tag="IW797_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ1223838.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4248..4730
                     /locus_tag="IW797_001052"
     CDS             4248..4730
                     /locus_tag="IW797_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ1223839.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4746..5507
                     /locus_tag="IW797_001053"
     CDS             4746..5507
                     /locus_tag="IW797_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1223840.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5638..6972
                     /locus_tag="IW797_001054"
     CDS             5638..6972
                     /locus_tag="IW797_001054"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ1223841.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7279..7764
                     /locus_tag="IW797_001055"
     CDS             7279..7764
                     /locus_tag="IW797_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HAQ1223842.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIKSEGELN
                     E"
     gene            7757..8440
                     /locus_tag="IW797_001056"
     CDS             7757..8440
                     /locus_tag="IW797_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ1223843.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8566..11211
                     /gene="polA"
                     /locus_tag="IW797_001057"
     CDS             8566..11211
                     /gene="polA"
                     /locus_tag="IW797_001057"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ1223844.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11256..12092
                     /gene="mutM"
                     /locus_tag="IW797_001058"
     CDS             11256..12092
                     /gene="mutM"
                     /locus_tag="IW797_001058"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ1223845.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12056..12685
                     /locus_tag="IW797_001059"
     CDS             12056..12685
                     /locus_tag="IW797_001059"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ1223846.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            12737..13069
                     /locus_tag="IW797_001060"
     CDS             12737..13069
                     /locus_tag="IW797_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1223847.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13381..13872
                     /gene="nrdR"
                     /locus_tag="IW797_001061"
     CDS             13381..13872
                     /gene="nrdR"
                     /locus_tag="IW797_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ1223848.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            13895..15268
                     /locus_tag="IW797_001062"
     CDS             13895..15268
                     /locus_tag="IW797_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ1223849.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15268..16194
                     /gene="dnaI"
                     /locus_tag="IW797_001063"
     CDS             15268..16194
                     /gene="dnaI"
                     /locus_tag="IW797_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ1223850.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16323..16763
                     /locus_tag="IW797_001064"
     CDS             16323..16763
                     /locus_tag="IW797_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1223851.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            16907..17608
                     /locus_tag="IW797_001065"
     CDS             16907..17608
                     /locus_tag="IW797_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ1223852.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17605..18726
                     /locus_tag="IW797_001066"
     CDS             17605..18726
                     /locus_tag="IW797_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ1223853.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            18741..19973
                     /gene="pepT"
                     /locus_tag="IW797_001067"
     CDS             18741..19973
                     /gene="pepT"
                     /locus_tag="IW797_001067"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ1223854.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20105..21013
                     /locus_tag="IW797_001068"
     CDS             20105..21013
                     /locus_tag="IW797_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ1223855.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21048..21320
                     /locus_tag="IW797_001069"
     CDS             21048..21320
                     /locus_tag="IW797_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ1223856.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21331..22377
                     /locus_tag="IW797_001070"
     CDS             21331..22377
                     /locus_tag="IW797_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ1223857.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            22628..25237
                     /gene="clpB"
                     /locus_tag="IW797_001071"
     CDS             22628..25237
                     /gene="clpB"
                     /locus_tag="IW797_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ1223858.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25388..26059)
                     /locus_tag="IW797_001072"
     CDS             complement(25388..26059)
                     /locus_tag="IW797_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223859.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(26052..26546)
                     /locus_tag="IW797_001073"
     CDS             complement(26052..26546)
                     /locus_tag="IW797_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ1223860.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26566..27786)
                     /locus_tag="IW797_001074"
     CDS             complement(26566..27786)
                     /locus_tag="IW797_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1223861.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            27933..29768
                     /gene="lepA"
                     /locus_tag="IW797_001075"
     CDS             27933..29768
                     /gene="lepA"
                     /locus_tag="IW797_001075"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ1223862.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(29862..30989)
                     /locus_tag="IW797_001076"
     CDS             complement(29862..30989)
                     /locus_tag="IW797_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1223863.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10324 a         5552 c         6636 g         8490 t
ORIGIN      
        1 tttttctgtg cgaaacagga aagcgaagtc tttttaattc aaataataat attatgtaaa
       61 caaaaaactt tccatgcgta attatacaca agcaaataaa tcgtcaaatg cgaacgcctt
      121 ctttatactg tttaggaacg aaaagaagga aggaagaaat acatggatta tcaagtactg
      181 ctttattaca attatacaaa aatcgaagaa ccagaggaat ttgccaaaaa tcatttagcc
      241 ttttgcaaat ctttggatct taaaggtcgt attctagtag ctgaagaagg aataaatggt
      301 actgtgtctg gaaccgttga aaacacgact gcctatatga aagcaatgaa agctgatcct
      361 cgatttgctg atacgatgtt caagatcgat ccaagcgaag gacatgcctt taagaagatg
      421 tttgtaagat accgtccaga gcttgtaagt ttaaagctag aagaggacat tgatccaaac
      481 caagtgacag gtgcctatct ttcaccgcaa gaatttaaag aagctatttt agatgaacag
      541 acagtggtga ttgatgcacg gaatgattat gaatatgact tgggacattt tcgaggggct
      601 gtacgcccag atatacgcag ttttcgagag cttccagact ggatccgcga gcacaaagaa
      661 gaatttatgg aaaaacgggt agtgacctac tgtactggtg gcattcgttg cgagaagttt
      721 tctggttggc ttgtccgtga aggattcaaa gatgtcggac agctgcatgg cggtattgct
      781 acttatggta aggatcctga agtccaagga gaattatggg acggcaagat gtatgtgttt
      841 gatgaacgga tcgctgtcga tatcaaccaa gtagataaac aaatcgtcgg tcgcgactgg
      901 tttgacggaa caccatgtga acgatatatc aattgtagca atcctgagtg caatcgtcag
      961 attcttgctt ccattgaaaa tgaagaaaaa catttaggag catgtagcat agagtgtgcg
     1021 aaacatccgc gcaatcgcta tgttcaagac catggaatct ctccagaaga agcagaactg
     1081 cggtttacag ctttaattaa atagaaaaca atagttttga ttgtcgacca tttccaatat
     1141 gcgctatact agcgttgagg atggtgatcc acatgaaatc attgatttat gctattgggg
     1201 atgtccatgg caaacatgag ctttttgaaa aacttattca ctattatgat ccaaagatcc
     1261 atcagcttgt tttattaggg gatctcaatg atcgaggtcc tcgtacaaaa gaatgttttt
     1321 tattaggaaa gcaattagtc gaagaaacag gagccattta cctccgggga aaccatgaag
     1381 aatatttttt gcaatttctg caatcaccgg aggactggta tgcgccttat atgagaaatg
     1441 gtggaaaaga aacgtttgaa agcctgattc acccaggggc atcggaggaa tactccccaa
     1501 ctgaaatctc gatgatgatc caatcgagat attcagaact gattaaattt ttagtccagc
     1561 gccccttgta ttatgaatgg gaaaattatc tttttgtcca tgccggtgtg gatttgacaa
     1621 agaaagactg gcgtaagaca agtctaagag actttatttg gataagggaa ccatttcatg
     1681 aagggaaaaa caatacaggt aagacaattg tatttggaca tacgattaca ccaatgctcc
     1741 atggagatat gcagacaaca gacctctgga ttcaggatca taaaatcgga atggacggtg
     1801 gggctgtatt cggagggtca ttgcatgggg ttgtttttga caaggaaggt attcttcaag
     1861 atattgaata tcagaacaac gaaggacctt ggcatggaat ctgactaatg gaaagaatga
     1921 aggagctgtg actttgttgc ggctcttttt atttttatag agaatattgt ttctttcagt
     1981 agtttaaccc aggataaaaa gtatgataga ataaagaagc aatttttagc agaaaagagg
     2041 cattttaatg acggaaaatt tgaaccagac gatcattcag cttgtacaaa aagaactttc
     2101 agactatcgt cctaagcagt tgactactgt acttaatttg ttaaatgaag gaaacactgt
     2161 tccttttatc gcgcgttacc gaaaagaaat gactggaagt cttgatgaag tccagattcg
     2221 tgaaatcgaa gagcggtatg cctatcttga aaacttagaa aaacgtaaaa ctgaagtgat
     2281 tcggctaatc gatgaacaag ggaaactgac tcccgagtta gaagcagaaa tcacccaagc
     2341 cgtgaaaatg caacaagtgg aagatttgta tcgtccatac aaacaaaaac gacggacaaa
     2401 agcaacgatt gctaaagaaa aaggacttga accactcgcg aagtggctga tgcagcttac
     2461 agatggagag gtgcaatctg aagcagaaaa atatattgac aaagaaaaag aagtctcttc
     2521 tgccgaagaa gcgttgcacg gtgctcatga gattattgct gaacaagtca gcgataatgc
     2581 aaaatttcgg acttggattc ggtcttatac gtataacaaa ggtatgtatg tcagcaatgt
     2641 caaggatgag caagcagatg aaaaaggcgt atatgaaatg tattatgact ttgcagaacc
     2701 cgtccataaa atggtttctc atcgaatatt agctactaat cgtggagaaa aagaagatat
     2761 tctgaaagta ttctttcaag tagatgaagc agcaatttta gcttacttgg atcgccaaat
     2821 agtcaaaaat cctgcctctc cttcttcttc attcgtcaga gaagcctatc aggatagtta
     2881 taaacgcttt attcagccag caatcgagcg agaactgcgc aatgaattga ctgaaaaggc
     2941 agatgagcaa gcaatcgcta ttttcggcga aaacctccgt aatcttttgt tgcaaccgcc
     3001 actaaaaggt aaagttgtcc tgggatttga tccggcttat cgtactggat gtaaactagc
     3061 agtcgtagat gcgacaggga aagttttagc aatcgaagtt atttatcctc ataaacctgc
     3121 agcgcaagca aaaagagaag ctgctggacc agcttttatc caattaatta ataaatatca
     3181 agtagatatg gtagctatcg gaaatggtac agccagtcgg gaatctgaac tttttgtggc
     3241 tgagcagttg aaatccgccg accataaaac ctattacgct atcgtcaacg aagcaggcgc
     3301 ttctgtttac tccgctagcg aaattgcacg aaaagaattt ccgcatctac aagttgaaga
     3361 aagaagtgct gtttctattg cccgtcgcct gcaagatcct ttagctgaat tagtcaaaat
     3421 cgatcctaaa gctgtcggag taggccagta ccaacatgac gtttctcaaa aacgtttggc
     3481 tgaacaattg gattttgttg tggaaacagc ggttaaccaa gtaggtgtgg atgtgaatac
     3541 agcaagcccg cagctcttgc agcatatatc aggattgaat aaaacaactg cccaaaacat
     3601 tgtcatttat cgtgaagaaa acggagaatt caccgcacga actcaattga aaaaagtgcc
     3661 acgcttagga ccaaaagcat atgaacaagc aatcggattc ttgcgagtcc ctggtgggaa
     3721 aaacattttg gacaacacag gtatccatcc cgaaagttat tctattgcaa aagaaatatt
     3781 aacttcagtc caactttcag aaaaagagct tggtacagaa gaagcagtag aaaaattgac
     3841 acgattatcc gtagagaaac tggctgaaag cctatcagtc ggagaagaaa cgttagccga
     3901 tattttagct gggctgacac aacctggacg agatatgcgt gatgagatgc cagcgccctt
     3961 attacgtaca gatgtattga gtatggaaga cttgaaacca gggatggaat tgactggaac
     4021 ggtccgtaat gtcattgact tcggcgcatt tgttgatatc ggtgtgaagc aagacggact
     4081 tgtccatata tcgaaactca gcaaaaaatt cgtcaagcat cctacagatg tcgtgtcagt
     4141 aggagatatc gtaacagttt ggatagaaca agtcgatacg aaaaaaggca gaatcagttt
     4201 gacgatgctt tccccttatg aagagtaaca atgaggagta acaaaaattg acacaagaag
     4261 aactgcagca gttagtagaa gagatatcat taaaaagctt ccagcttcct ttcaagcacc
     4321 aagcaatgtt caatccacgc ttacgtacaa ctggcgggcg ctatcattta aacgatcatc
     4381 atttagattt taatgcgaag ctatttgaag agtcagacga agagacgatt gcaggaatca
     4441 tcaaacatga actttgccat tatcatcttc atctagctgg aagagggtat cagcataaag
     4501 atatggattt caagtatctt ttgaaaaaaa caggagggtt gcgctatgca ccagccttga
     4561 cgaagaaaca gataaagatg gagtattatc gatgccaaga ttgttctgcc acgatctgcc
     4621 gcaagagaag aatcgatgta cgcaaatatc gttgtggaag atgtcatgga gaaatcaaat
     4681 ggattgagac aaaaagagaa gactttaatg aagcacaaaa aatcagatga aaggatagaa
     4741 ggcctttgga aaaaagtgag cagaggaatc taaaagactg gaatcagcta ttaaattttt
     4801 tagccgcacc tgaagaggga aattttgagg aactgacggc acctacactt attttagctg
     4861 gaaactgtct tcctatctta gctgataaag cagcacaaat gtatttaaat ggccaagtag
     4921 atcaattgtt tttggtcgga ggggtggggc atgcgactag aatcctttat gagaattttg
     4981 aaaagcaagg atttcatttt gaagatggca tgagtgaaag tgaaatctgt cgtcagtatc
     5041 taaaagaagt ctatgatttg cccgataagg cttttctgat agaatcgaag tctacaaatt
     5101 ctggagagaa cgccactttt tcactggaga ttcttcattc cttaggagct gtaccagaaa
     5161 aaatcttatt gatgaatgat ccgacactgc aacgcaggac tcgagcaact tttgagaaag
     5221 tatggcaaaa tgaacaaact attttcgcga atgctgtgcc atttgtgccg gaaatccttc
     5281 acttttctga agagatcatc tttacagcaa aagaactgaa tcatcaatgg cctaaggaat
     5341 atttccatgc gctagtatta ggagaaatgg aacgtctcca tgacgatgag aacggctatg
     5401 gtccgaaagg aaaagatttt attcctcaca ttgatatatc agaggaagtt tggtcttctt
     5461 acaccagaat taaagagtca ataaaaacgg atttttctcg tacttgacca ttttagcaag
     5521 cgaatcctcc tttccaaata aatttggttg aaattttcaa tcgtttataa gataatgagg
     5581 agcgtgttga atgtaatagc taaaagaatg gacgctagat aagagaagag gattcaaatg
     5641 gaaaatcaaa ataaattgta tcattttgtt ggtatcaaag gttcaggaat gagctccttg
     5701 gctcttgttt tacatgaaca aggtctaaat gtccaaggat cagacattga aaaatatttc
     5761 tttacacaaa gagatttaga aaaagcaaat atcacgattc tgccatttaa cgcagataat
     5821 gtaaaaccag ggatgacgat cattgcagga aatgcatttc ctgattcaca tgaagaaatc
     5881 cagcgagcaa aagaattagg tttggaagtg attcgctatc acgactttat tggtcatttt
     5941 atccaaaatt atacgagtat cgctgtaaca ggctctcacg gaaaaacaag tacgactgga
     6001 ctgctctctc atgtattgag tggtgtccgt ccaacaagtt atctaattgg agatggaaca
     6061 ggacatggtg atccgcaagc ggaattcttt tcatttgagg cctgtgaata tcgccgtcat
     6121 ttccttgcct attcaccaga ctatgcaatc atgaccaata tcgattttga tcatccggat
     6181 tattatacaa gtatcgacga tgtatttaca gctttccaaa caatggctgg acaagtgaaa
     6241 aaagctattt ttgcgtatgg tgacgatgct tatttacgca aattaaaagc aaatgtgccg
     6301 atttattatt atggtgtcac agaaaacgat gatatccaag cacgaaacat cgaacgcaca
     6361 acttctggtt ctgcttttga tgtctatcat ggggatgaat ttgttgggca cttcactgta
     6421 ccagcattcg gtaaacacaa tatcttaaat gctttaggtg ttattgcagt agcttatttc
     6481 gaaaaactgg atctgaaaga agttgcagaa gaaatgctga ctttcccagg agtaaaacgt
     6541 cgattcagtg aaaagatcgt agcagacatg accgtagtcg atgattatgc gcatcaccct
     6601 gcagagatca aagcaacgat tgatggcgca cgccaaaaat atccagataa agaaataatt
     6661 gcagttttcc aaccccatac gtttacgcgt acgattgcat tgatggatga atttgcagaa
     6721 gcacttgatt tagctgataa agtttactta tgcgatattt ttggttcggc tcgggaagaa
     6781 caaggaaacg ttaagattga agaccttgga gcgaaaatca aaaaaggtgg agaagtcatc
     6841 aaagaaaata atgtatctcc attgttagat tatcatgatg cagtagtaat cttcatgggt
     6901 gctggagatg ttcaaaaatt tgaacaagcg tatgaaaaac ttttaagcag cacgacgaaa
     6961 aatgtactgt aatacaatta ctgcagtgtc cttataaaaa tagaaagagg gggagtggca
     7021 aaaatcttgc caagccccct tttttgcaac ttatgataaa cggtgttcaa aagcttgcat
     7081 ctgcggtaat aagcccaatc aaatcaaaaa catgagaagc tgttttttct tggctgttct
     7141 tattatttga tgcaggacag ctttttcacg acctctgtaa actataggaa tctgttaaat
     7201 caagggagca gaacttgcct tttacgctac ttttggtaca ataatgtatg attaaaaata
     7261 aactggaggg tttctatctt gaacataggt aagccgagaa aactaggaaa aacaataaat
     7321 gaaatcgagg aaggcgactc gttatcctta acagaatcta ttgaagataa ccagcttttg
     7381 ctgtatttgg gattgacgaa tgatgcaaat cctttgtata tccaacatga ctttgcacaa
     7441 aaaacagaat atcaacagcc aatcgttcca tcagtgatgc tgatggggat catcacaagt
     7501 gcaatttcaa agcatcttcc tggaccagga tcacatgttg tcaatttttc tgtcaatttt
     7561 attgaaccgg ttttccatta tgaaacactg acttttcagt ttgaggtcat aaaagtagat
     7621 aagatgaagg atgtggtgac gatttctgta gaagcggaga atacgaacga agatcgtgtg
     7681 ttggatgctg tcgttatggt ccaaccgcca caagttcctg aattggaaat agcaatcaaa
     7741 agtgaaggtg aattgaatga ataatccaag tacatcaatg tcaggtatca atcgttttta
     7801 tgcaaaagtt tatggatttt taggtatggg acttgccctt agtgcagtca cttcttactt
     7861 ggctctttgg gtatttcctt tgcaattagc aacttttatc aataattttc cactaggctt
     7921 tgtgggtctg tggatcgttg aactgatttt agtaatggtt ctaggcgtaa aagcacaaaa
     7981 gaatccgagc ttagcaatcg gtggatttat tgcctactct cttttgaatg gcataacatt
     8041 agcggttact cttgcatact acgatattgg aacagtaacg caagctttct tgacagctac
     8101 tttgatgttc atggggatgt ctgttgtcgg agtaacaaca aaaagagatc ttagcggggt
     8161 tggaagagca ggacaggcag ctttaatcgg agtgatcatt gcactagtct tgaacatctt
     8221 cattttgaga agtagcgtag ttgagctgtt catctcccta ctgttagtcg tcatctttgc
     8281 agggattaca gcttatgata accaaagcat ccgtaatatc tatgtccaga caaacggaac
     8341 agcacatagt ggaattgctg tattcatggc attgaagctg tatttggact ttatcaactt
     8401 gttcttggcg tttctgagaa tctttggacg gaacaactaa aaaagaaaaa aattcatcag
     8461 ttggggctat accgaattat ttggtatggt cccgcttttt cttttcgcat tccgcaggat
     8521 tctttgttag aatgaataag aactttacag taaggagtta agaagatgac aaaaaataaa
     8581 ttattactcg ttgatgggaa cagtgtggct tttcgcgcat tctttgcgct tcacaattct
     8641 cttgaacgtt tcaaaaatag aaacggtctt catacaaatg caatctatgc attcaatacg
     8701 atgtttgaaa atgttatgca aaaagagcaa ccgactcatg ttctggttgc gttcgatgca
     8761 ggaaaaacga cttttcggac agaaatgtat gcggagtaca aaggtggaag atccaaaaca
     8821 cctggcgaat tcaaagaaca aatgccatat atacgtgatt tgttgacagg actaggggta
     8881 cagtattacg aattgcccaa ttatgaagca gatgatatta tcggtacttt ggcagaaaaa
     8941 gtagacaaag atcaatttga tgtcgtcgtg ctttcaggtg atcgggattt gactcaatta
     9001 gctagtaaag aagtaaaagt ggatatcact gtaaaaggtg tcagcgatat cgaaagctat
     9061 acaccagaac atgtagcgga aaaatatgat gggttgacgc caaaacagat tattgacatg
     9121 aagggactag ccggagatgc ctctgacaat atccccggtg tgactaaaat cggtgaaaaa
     9181 acagctatca agctattgaa acaatatggc tcagtcgaag gaatttatga acatatcgat
     9241 gaaatgaagc aaagcaaaat gaaagaaaat ctgataaatg acaaagagca ggctttctta
     9301 tcgaaaaaat tggcgaccat caatacttca gcgcctgtgg atgtctctat cgactcattg
     9361 aagtatgaag ggaaaaatct cgataaactc gttccgtttt ataaagaaat ggatttcaat
     9421 cagttccttt ctaaattgaa tatcgtagaa gaagaagtaa aaatggatga tattttgttt
     9481 gaagtcgttc atgaattcaa agaggaaatg tttactacgg atatggcgtt gtacgtggaa
     9541 atgatgggcg acaattatca tacagaggaa atcgtcggag tagcgtgggg gacagaaaag
     9601 aaaatttatg tcacgaacga tctatctgta ttcgaaaacc gagcgttcca ttcttggatc
     9661 actgatccga ctcggctgaa aaaagtatat gatgcgaaac gaacttatgt tgctttaaac
     9721 cgctatactg gaaaaacgaa aggaatcgat tttgatgtct tgctaggtgc ataccttctc
     9781 gatacgaatg aaaaaagcac agatatcgaa ggaatagctg ctcattacgg atacaacgat
     9841 atccaatcag atgaagctgt atacggcaaa ggggcgaaaa aaggtttacc agaagaagag
     9901 gaactctttt ttgctcattt agcgcgaaaa gtagctgcaa tcaatgcgtt gacaccaaaa
     9961 ttgagccaag aactggcaga taaaaaccaa gaagatcttt tccggaaaat ggaactgcca
    10021 ttgtctatca ttttggcaga gatggaaata agcgggatca aagtagatgc gactcgctta
    10081 caagaaatga agggcgagtt ctctgctcgc ttaagagaga tcgaacaaaa aatctacgaa
    10141 gaagccggcg aagaattcaa tttgaattca ccaaagcaat taggagtgat cttatttgaa
    10201 aaaatgggat tacccgttat caaaaaaaca aaaactggat attccacagc cgtagatgta
    10261 ctggaacagc ttcgtgaaca agcaccaatc gtagaggata tcctcaccta tcgtcaaatt
    10321 gcaaaaattc aatctaccta tgtcgaggga ctgctgaaag tgatccaatc agatggaaaa
    10381 gtgcataccc gttatgtcca gacattgacc caaacaggac gattaagctc tgttgatcct
    10441 aatttgcaga acatcccgat tcgattagat gaagggagaa agatacgtca ggcatttgtt
    10501 cctagagaaa aagactggct gatttattct tctgactatt cacaaatcga gttacgcgta
    10561 cttgcgcata tctcaaatga tgaacatttg aaagctgcct tcctagaagg tcaagatatc
    10621 catgccagca cagcgatgcg tgtatttgga atcgaaaagg cagaggatgt cacaccaaat
    10681 atgcgtaggc aggcaaaagc agtgaatttt ggtatcgtgt atggcatctc tgattacgga
    10741 ctttcacaaa atctaggaat cagccgaaaa gcagcacagc aatacatcga tacgtacttt
    10801 gaaaaatacc caggtgtcaa agaatatatg gaaagaatcg tacgcgaagc aaaagaccaa
    10861 ggctacgtcg aaacgctgta tcaccgccgt cgatatctgc cagacatcaa ttcaagaaat
    10921 tacaatatcc gttcttttgc ggaacgaaca gctatcaata caccaatcca aggaagtgct
    10981 gctgatattt tgaaaatcgc gatgatcgaa ttggataaac gattaaaaga aacaggtctt
    11041 caagctacga tgctgttaca agttcatgat gaacttgtct tcgaggtacc ggaaaaagaa
    11101 cttgaatcat tagataaatt ggtgaaggaa gttatggaac aagctgtttc tcttcatgta
    11161 ccactgatca ctgacagcag ttggggaaaa acgtggtacg aagcaaaata aacattgttt
    11221 aagacagagc aatataatga aggtgaaaag aaaaaatgcc agaattacca gaagtagaaa
    11281 ccgttcgtaa aggattggaa cggttagtag taggaaaaac gattcaaaaa gttcaggtct
    11341 tgtggcccaa gatcatcgag caaccagaga cacccatctt tgaggcttct cttgttggcg
    11401 aaaccatcca atccattgga cgtagaggga agtttttaat ttttcatctc gatcattgtg
    11461 aactgatctc acacttgcga atggaaggaa aatatcaatt tacgaaagaa aatacaccaa
    11521 tcgataaaca tacgcatgta cttttctttt ttgaggatgg aagccaactt cgctacaacg
    11581 atgtacgaaa atttggtcgt atgacaatcg tcgaaaaagg agcaagtgca acttaccgag
    11641 gaatcatgaa attgggacct gagcctttgc cagatagctt cttattagca gatttcgcaa
    11701 atggactgaa aaagtcccac aaagcaatca agccattgct gctagaccaa cgtctagtga
    11761 ctggtctagg taatatttat gtagatgaag cattatggga agcgaagatc catccagaac
    11821 aaccggcgaa cacactgcgc cccaaagagg tagaacaatt acgtctatcg atcattgatg
    11881 ttttggatcg tgcaatagaa gccggaggta ccacgattcg cagttatttg aatgcattag
    11941 gagaatcagg cggatttcaa gtagcactgc atgtctatca gcaaactggc aaaccatgta
    12001 tacgttgtgg aacgccgatc gtgaaaacaa aagtggcaca aagggggacg cactattgtc
    12061 cgaattgtca aaggctaaaa atcgcaaagt aggatttgtt ttaggtttga cgggtggaat
    12121 agctaccggt aaaagtacgg cagccaaagt ctttcagtct catggatttc ctttgatcga
    12181 tggcgatgtg attgccagag aaactgttga gcctggaacg cttgctctga ggaaaattgt
    12241 atcagtgttt ggccaagagg tattgcagcc tgatgggcaa ctagatcgag ggaaacttgg
    12301 gatgattgtt tttccatcaa aggaaaaacg gcaaaaactt gatcagctat tagatccgtt
    12361 tatccgaaaa gcgataaaag atcaaatcgc ctctctaagt tccaatcatc ctttagtaat
    12421 tgtagatatc ccattgctct acgaaggtca ttatgaccac tatatggatg cagtagctgt
    12481 cgtttatact acgccagaaa ctcagcttca gcgtttgatg aaacgtaatc aactgacgct
    12541 agaacaagcg cagcagagaa ttagtagtca acttccaatc gaagaaaaaa agcagcgtgc
    12601 ggatattttg ttcgacaaca atggaacaaa agaaaaactt gtgggacaaa tcgaaaattg
    12661 gctttttgct aatcgctttc tttgagtatc tgtgtcaatt ttgctatcct gagtatatag
    12721 aaagtgaggg atagcgatgg gatcattaaa aaatcaaaag gataaagtcg taggtaacgc
    12781 aaaagaaaaa atcggcgaga tgctcaataa agaaaatcta atcgatgaag gtcgtacgca
    12841 atcagaaaca gcagaagaaa aagaaaagca gtaccaagca agaatcgatg aaatagaaga
    12901 ccagcaggca gaagaagaag cgaagcggca agatttgact caagaacgaa aaaatccgac
    12961 acctgatcgt agtaaaacac cgctcccaga aagtgaccac atttccgatg agcaaaaaat
    13021 cgaggaacag cgaatgaaac actatacagg aatcgaagag aagttataaa atagtaaaaa
    13081 atcgagatgt gacttgaagc agatgagaag gtgttcatct gcttcaagtt ttttgtgcta
    13141 gatgactaaa tcagcttttg tgtaagttat ttctcagtaa tcaaggtgat cagtattgga
    13201 aaattgaaat ttagtaagga caggggacac aattacgcat gaaagtagaa aaaattgttc
    13261 ttatatccgt gttttttggg taggacagtc gcgtttgtga tttgtttcgt gttataatac
    13321 aactagttgt aaagaaagac acatccttta tataaaatgc gatgcaagag gtgagacaaa
    13381 atgagatgtc ccagatgtca acataataat tcacgagtaa tcgacagccg tcaagcagat
    13441 gatggacgtg cgatccgtcg ccgtcgcgaa tgtgagaatt gttcgttccg attcactaca
    13501 tttgaacgaa ttgaagctgc tccgttatta gtaatcaaga aaaacgggga acgcgaagag
    13561 ttcaatcgcg ataaaatttt aagaggactc atccgttcag cagaaaaaag acctgtagct
    13621 atggaacaaa tggaacaaat cgtcaaccat gtcgaaaacc gcgtacgcag tctcggagaa
    13681 aacgaagtat cgacgaatct aattggtgaa tatgtgatgg aagatttagt tgatttagac
    13741 gaaatcgctt atattcgttt tgcaagtgtt tatcgccagt tcaaagatat gtctgttttc
    13801 ttaaaagagt tgcaagatat cgtggataaa gcaaaagaaa ataataaaga gaataataaa
    13861 gaaaacaatt aagaaacaaa aaggaggcat ttccttggaa aatgcttgga aagagcttca
    13921 accaaaaaat atttaccaag gaagaaaagc ggctccgctg agagaagaca gcaagaacgc
    13981 cttgctgtat ctttatcagc cactaatcgg cggagaagcc ttgagtatct atttcaccct
    14041 gttatcagaa atctctcaag aatctggcaa aggtcctgaa gggatgcatg cagatctgct
    14101 tagtagtcta ggctgcggta tcccgcaatt ttatgatgca agaaaaaagc tagaaggaat
    14161 cggcttactg gatgtttatt ttaaagaaga tagtgatctg ggacctcgtt ttatttatga
    14221 gctgttagaa ccactcattc ctaaagcatt tttcaaagac tctgttctgg cttttcttct
    14281 tctggaaaaa gttggtgaac gacgattcca gaagcttacc caattctttg aacctaaaag
    14341 gcatacagca gagggatatc aaaaaatcac aaaaaaattt ttagatgttt atcgcttcaa
    14401 tgaagcagtg tacgctgcta atcaagaaaa catcgaatcg ctgaataaat cttttgaaga
    14461 atctgaatca gtagcattac ttgaagcaga ttcgacattc gattggcagt ttttgaatga
    14521 ttggctggca aaaaaacata ttgcaagtct tgatgatcca atcatcaaac aagtcgacat
    14581 gtatcatcag ctttacggtt tcgatgaatt gacacttggc gaactgatca gtcaatctta
    14641 tgactttagt gaacaaaaag tatctgaaaa agaactgcaa aaagccattt tggcaatgca
    14701 gagacgtccg gtgaagcaaa caaaagaaac agttgcggtt tctgaaaaca gtaatgaacc
    14761 ctcgtcaaac gaacatttga caaacgcagc gcttcagctg ataaaagaag cacagagcat
    14821 tccaccgatg aaatatctgg aagcaatcaa gtcagaaaaa ggcggatatg tttctaaaca
    14881 agaaaactgg ttactgcaag atcttgtttc tcaatcaggt ctttccagta gcgtgatcaa
    14941 tgttttgctg aattatgtac tagtgatcaa gaataatgct agcttaaatg ccagttatgt
    15001 aaatacagtg gcaaatgaat gggcacaaaa gaaaatcgcc acagcagaag aagcgatcca
    15061 gcacattcgg caaataagta atcaagcaaa acaatcaaaa caaaagaagc aaacaaatta
    15121 tcaaactggc aagcgaaatg ttcgacggga aaagcttcct gattgggtca accagccaaa
    15181 agatgaaaaa cagatcagca gcgagaaaaa agcagagatt gaccgccgtt ttaaagaata
    15241 cctggcaaaa aaggagggtg atatctagtg gaagacgtag gcaaagaatt gaagaggatc
    15301 atcagcaaac gcaattatca agaaaagtat gctgaaatga tcgctgaagt cttaaatgat
    15361 gcggatgttc aagcttttct aagcgcacac caagaagaat tgacacaagc ggatattgaa
    15421 aaaagttacg ctaaattgta tgaattcgtg caagaaaagc gtaaatatca aaaaaacgat
    15481 ccaacgatga tcgctcccgg ctatgagccg aagctgacgt tgaattttca ttatgtggat
    15541 gtcacttatg taccaacgga agcattgtta gcacggcaaa gacaagaaga aatcagaagc
    15601 cgagtaaaag caatggatat gccaaaagat attcaatcgg cttcttttga aaactttgaa
    15661 cgtacagatg gacgaggata tgcgagctta gaagccctag attttgtaga gaaatatact
    15721 gctgatccaa aagggtatca taaaggactg tatctagtag gaagttttgg tattggaaaa
    15781 acatatttat taggagcaac agcgagagaa ttagcgattg ctggattcac cacgacgcta
    15841 gtccattttc cgacatttgc cgttgagatg aaacaggcaa tcggaaaaga tcaagtggca
    15901 gaaaagttag atgcagtgaa acgttcacct attttgatga tcgatgacat tggtgcagat
    15961 gcgatgagta gttggatacg agacgaagta ttcggtgtta tccttcaata tcggatgcag
    16021 gaacagttac caaccttctt ctcatcgaat tttacaatgg atgaattgga acagcatttg
    16081 tccgtcactc aacgtggaga tgaagagccg ctaaaagcga aacggataat ggaacgcata
    16141 cgttatttga caaaagaaat cgaaatgact ggtcgcaacc gacgcaatgg ttgaacagtt
    16201 gacaagtcca actgacattt gacctagtag taaaagcagg tcagcgaatc attatatgga
    16261 cagaaatgac cggagacaat caccgcaaca agtaatgcag aaacttaaac ggaggatttt
    16321 ttatgagggc tattttttct agtatatctg tagttggtct attttttctt ttagcaggct
    16381 gcggtagcgc agatagcaac acaggttctg ttgattcgat tgatgcgggt caaacagtaa
    16441 aaacaagcag tacgaaaacg gagagacaaa gcatatttgt cggaacgatt gaaagtatta
    16501 ctcaagtaga agcagaagaa gaaacgcttc agttgaaact cgtcgatgtc atggccaaag
    16561 aagatccagc gaatatggga acgtcattca gcaatgacgg agtgatctta aatgctactg
    16621 ctgaaaaaat aagcgtggac ataaatgaat tgaaaatcgg tgacaaagtc caatttacat
    16681 tagttgagat gccgatcatg acgatgtcta tcccaccgca agttcctgga gattcgatta
    16741 ttgatgtttc attgaacaaa taaccaataa cattttttca aaaataagaa aatgttgttt
    16801 tatagaacga aaaggtatat tttttaagga gatatgggaa tttttttatt cctagtctcc
    16861 ttattttatt tggaaaatgc tataattatc tagaaggagt gaagctatga accatttaga
    16921 tttatctcag cgcttagccg cagtgggtga ctttgtccct gaatccgctc gtttagcaga
    16981 cattggatca gatcacgctt acttacccgt tgcattgata ttaagaggaa aaatcgaata
    17041 tgcagttgct ggtgaagtcg ttcaaggtcc ttatgaatca gcacgtaagc aagtcaaaaa
    17101 agatggatta gaagaccgta tcaccgtacg ccttgcaaat ggtctagatg ctatccagcc
    17161 acaagatggt attacagctg tcacgattgc gggtatggga ggttcactga tccgagatat
    17221 cctagaagct ggcaagttga atcaacgctt aagcggacaa gaacgtttga tccttcagcc
    17281 aaacatcgga gagcgtacat tacgatcttg gctgcaagaa aatcagtata aaatccaatc
    17341 agaaaaaata cttgaagaaa ataaaaaaat atatgaaatc attgtagccg aaaaagaaca
    17401 aaaaccagtt acttacaatg aaatggagct gatgttcggg ccatttctgc ttgaagagaa
    17461 aagtgagatc ttcaaatcaa aatggacaag agagcttcaa caaagaaaag ctatccgtga
    17521 acagctcaaa caagcagcta atcaagaggc acgtcaaaaa caaatcgagc aggaaatcca
    17581 aatgattgag gaggttttaa agccatgaca atcagcggac gcactttcat ccaaaagttt
    17641 gaaagctatt gtcctcagtg gctagctgaa gaaggagacc cagtcggact gcatatcgga
    17701 acattggata agccaatcca acgagtaatg atgacattag acgttcgtcc agaagtggtc
    17761 gaagaagcaa tcaagaaaaa aatcgatttg ctgattgcta aacatcctcc aatctttcgt
    17821 cctgtaaaac ggctggttac tgatcagcca caagaaaaaa tgtatgcaga tttgttgaaa
    17881 catgatattg ccgtctatgc agcacacacg aacatggata ttatttggga tggactgaat
    17941 gactggtttt gtgaacttct cggtattgaa gtggagagtt atttagtaaa gactcatgag
    18001 attcattata aaaaactggc cgtttatgta cctgtggatc atgcacagaa aatgcgtgaa
    18061 gtattagctg cagctggtgc tggtacacaa ggtgattata ccggtacaag ttttacctcg
    18121 attggacacg gtcgatttac tccggaagct ggtgcacaac cagcgattgg gaaagtaggg
    18181 aagacagaac aagttcaaga ggcaaaagtt gaggttattt tgccggaaac tatcgaaaaa
    18241 caagtgattc aagccatgcg gtcagctcat ccttatgagg aacctgctta tgatcttttc
    18301 gctatagatg aaccagtcga aatgtttggg ttaggacgag taggtgaatt gccgcaagaa
    18361 atttctatag aggcatttgt agaacaagta aaagaagcgt tccaactaga tggactgaga
    18421 atagtccagc caaaaaatgc aaaatcctct gtgaagcgca ttgcgatttg cggtggaagc
    18481 ggtgaaaaat tttatccgca agcaatcgcc caaagagcag atgtctatat cacgggagat
    18541 atctattatc atacggctca tgatatgcaa agcgctgggt tgattgcaat tgatcccggt
    18601 cattatattg aatcgttatg caagcaacga tttatcgaga aatttgaatc ttggaaacaa
    18661 gaagaaaatt gggatattaa ctttttcgtt tctgaaacaa atacaaaccc atttcaattt
    18721 aattaaaagg agagaatact atgtacgaaa atcttttgcc acgtttttta cgatatgtca
    18781 aaacagaaac acgctccgat gcaacaagca aaacgactcc atcgactcag acacaagttg
    18841 cttttgcgca agaattgaaa aaagaattag aagagttagg cttagctgat gttttctata
    18901 atgaagataa cggattcgtt atgggaacat taccaagcaa tgttgatcat gaagtgcgtt
    18961 ccattggatt tatttcccac atggatacag cagattttaa tgcagtagga gtcaatccac
    19021 aaatcattga aaactatgat ggcgagtcta cgatcgtact agatgcggaa gaacgctata
    19081 cattgaatac aaaagatttt ccaaatttga aaaattatga aggccacaca ttgatcacaa
    19141 cagatggcag tacattactg ggagcagatg acaaatcagg gattgcggaa atcatgactg
    19201 caatggagat cttgatcaat aatccttcta tccctcacgg taccatcaaa gtggcttttg
    19261 gtcctgatga agaaatcggt gtcggagcgg ataaattcga tgttgagcaa ttcgatgtag
    19321 attttgctta tacagtcgat ggtggtcctt taggtgagtt gcagtatgaa acgtttagtg
    19381 ctgctcaagc aaatattacg atccaaggga aaaatgtaca tccagggacg gcaaaagata
    19441 caatgatcaa tgctctacag ttagcaatcg attttcataa tcagcttcca gccgatgaag
    19501 ttccagaaaa aacggaagga tacgaaggtt tcttccattt gatggcattg gatgggaatc
    19561 ctgaagaagc ttctatgagt tatatcattc gcgatcatga tcgtgaaaaa ttcgaagcac
    19621 gcaaagcaca aatcacacat atccaagaag agttgaatca gcgatttgat caagaacgaa
    19681 tcaaagtaga tatgtatgat caatattaca acatgaaaga aatcattgaa aaagatatga
    19741 gcatcgtgga attagcgaaa caagcaatga tcgagcttgg tatctctcca gtgatcgaac
    19801 cagttcgtgg aggaacagat ggttcaaaaa tctcttacat gggtattccg acgccaaatc
    19861 tatttgccgg aggagaaaat atgcacggac gattcgaatt tgtttcttta caagtaatgg
    19921 aaaaagcaac agacgtgatt gtaaaaattg cagaattgaa cacaaagagt taaaatcgaa
    19981 aaagaacagc ttatgacaag gcgttcgtcc caatgatgga atgtcatctg tctaaggcta
    20041 aagggcagga caatggaatt ggttgtcccg ccctcttttt tgaagagtaa ataggaggta
    20101 aagaatggaa aagctgctag ttttttataa caaaagttca gggaaagatg agggtgagaa
    20161 attagcacgc tggtttcaag attatgtaaa agaaaagcag ccagaattat atgtagaact
    20221 agctgaaact ggcccatcga ttgatgatgc ggaattagtc aagaaagcag aagccataga
    20281 agctgatact ttagtaatta taggtggaga cggcacgatt catcatatcg tccaagcgtt
    20341 ccaaaaaaca ttatctcaat accatgtcgg attacttcca ggtggaactg tcaacaacct
    20401 tgcacgtgtt cttgagattc ccctcaaaaa agaagaagct gctgacgtga tcctggaagg
    20461 caatgtacgg aaaatagact atggtcaagt caatgatgaa gtcattatct ctacactgac
    20521 aatcggaatc ttagcagata cagcagtttg gatcagtcaa gaagaaaaac agcgttacgg
    20581 ctcgtggatc tttatcaaga agttttttaa gttattgctg aagaagaaaa aatatcattt
    20641 ggatttagaa acagacgagg agcattggaa aggaaaatcc caactgttga cagtcacgat
    20701 gtctaactcc gtgggcggtt ttacaaattt tgacgactca gcgaccctag atgatggaaa
    20761 atttcatgta acgatcgtac cttccttaga tattttccga tttatttttt atttgcctag
    20821 aatcatgaga ggcaaattct atacgatccc cggcattact tatttcacag ccaaacgcat
    20881 gaaaatccaa gcgcaggaaa aatctgtggg cactcgaacg gatggagaca cgacagatga
    20941 tcttccagtt gaattatctg ttgtatcaaa aggattaaca gtttattcac caaagaaaga
    21001 agaaaaagct tagaaatagt agttaacaga aagttggaag aagagtaatg gatgtattgt
    21061 tacacaatct tttgtggttt taccttttca ttgtgaattt ttacttattt tgtctgatgg
    21121 ggtatgataa acatcaggct agaactagcg gctggcgtgt tcctgagaat aatttgatat
    21181 ttgtcagcct gttaggcggt ggtttgggag gattgatctc tcgaaaagtt ttccatcata
    21241 aaactcggaa aaagaagttt actataggat tcgctgcagg tgtacttgtt gatttgattc
    21301 tcatttattt tttccattaa ggagcaggag atgtcgtcta aaggaacgaa aactaaaatc
    21361 attctgaatc tgcttttgct gattgtgatt tttgccatta tttattattt aatcaatcaa
    21421 tcatttgcag atatctttgc cgaattgctg tctaccagtg ttgttgtttt ggcagcggct
    21481 gttatattag ggacagttta ccaaatcgct gaagggcgct caatcaaaga aattgctgaa
    21541 cctttcgcgc cgaattttac aacgaaagat gggttcttga cctctagcta tattgcgttt
    21601 taccgaatcg tttcgtttgg tacaggaacg ctgatctcgg agatttattt ttataacaaa
    21661 aaaggaatga agctatccca gggagcaggg gtgacagcac tccatatgat tatgtacaaa
    21721 ctggcagtga tcacttatgc attagctggc ttggtcatcc aattctctct tttttattct
    21781 aatggtccat caatgatctg gtttatcatc gctggtatcc ttttgacgat cgtgattgtt
    21841 gcctttttat tggcgatgtc cgtcagcttg aatgttcaaa tattttttgt cagcttatcg
    21901 aataagttat ttaaaagtga gcgtttgcgg aaatgggtgg atacatgcaa tcagcagatc
    21961 tactctttaa gggaaacggt ccaaaccatt atccaagaca ggactgcgct attgcggatt
    22021 tatctatgga atatgttaaa acttgtattt tggtatgtac ttccttattt gattctgatt
    22081 gaaaaccatc ctaaaataga ttttctactg accatttcgt ttatcagctt tgcagtagtg
    22141 ctttcagggg ttattcctac gcctgctggt attggttcct ttgaatttgt ctatttgtta
    22201 ttattcagac cagttgttgg aacagttgac gcagtttctt cgcttttact gtatcgtttc
    22261 agttccttta tcttgccatt tctctatgga tttgtgtatg tgattgctga acggcgaaga
    22321 gtgatcaaag aagaaataat agaagtaaaa aaagagaata aagaaaatca agggtaggtc
    22381 ttttataaga tctacccttt ttatcaaatc aaagaaaaac gttatggata agataatttt
    22441 acgtttgttt ggctcttttt ttatatacat acaatcaatt ttgttttttt aagatttata
    22501 taagtcgaga aaaaaagtca aaaaaccatg tgtatatttt gaccaaaatt gaccaaagcg
    22561 atataatagt ttttgtaaag atcaaagaaa taagtgatct tacctttaat taggagggta
    22621 tacctgtatg aatattgaga aaatgactac gacgcttcaa gaagcgattg ctgaagctca
    22681 acagattgcg gtcactcgaa aacatcaaga tatcgacatt gcccatgtat ggaaaatctt
    22741 tttacaacct aatcattttg gtcgaaattt ctatacggat gccggattgg atgtcgaatc
    22801 ttttgaacat gaaattgatc gtctactgga tgagtatcca gttgtttctg gtggaaacgt
    22861 ccaatatgga caaaacctca gccagaattt ctttcgatta ttgaatgaag cagaccaaat
    22921 acgtgaaagc ttcggcgacg agtttctttc aacggaagtc gttattttag cgttgatgaa
    22981 attaaaaaat tatccgttaa ctgtttattt gaataaaaac ggattatctg aaaaagaatt
    23041 gcgtaaaaac atcgaagaga tgagaggagg agaaagagtg acctcacaaa accaagaaga
    23101 acaatacaaa gcattagaaa aatacggcgt tgatttagta cagcaagtca aaagcggaaa
    23161 aatggatcca atcatcggtc gtgatgaaga aatccgtgat gtcatccgta ttctttcacg
    23221 aaaaacaaaa aataatcctg ttctgatagg agaacccggt gtagggaaaa cggcaatcgt
    23281 tgaagggctt gctcaacgta tcgtacgcaa agatgtgcct gaaaatttaa aagataaaac
    23341 gattttttca ttagatatgg gtgcattgat cgctggagca aaattccgtg gtgaatttga
    23401 agaaagacta aaagcagtct tgaaagaagt caaaaaaagt gatggacgta tcctcttgtt
    23461 catcgatgaa atccacaata tcgtaggggc aggaaaaaca gaaggaagta tggatgccgg
    23521 aaatctgttg aaaccaatgc ttgctcgcgg tgaattacat ttgatcggtg ccacaacttt
    23581 agatgagtac cgtcaatata tggaaaaaga caaagcgttg gaacgtcgct ttcaaaaggt
    23641 attagtcaaa gaaccaacag tggaagatac gatcagtatt ctgcgtggat taaaagaacg
    23701 ttttgagatc caccatggtg ttaacatcca tgacaacgca ttagtagcag cagctacttt
    23761 atctgaccgt tatattactg atcggttctt accggacaaa gcaatcgatt tgatcgatga
    23821 agccagtgca acgattcggg tagaaatgaa ctctatgcca acggaattag atcaagttac
    23881 tagacgtttg atgcagttgg aaatcgaaga agctgcactg aaaaaagaat cagatgatgc
    23941 aagtaaaaaa cgcttgaaaa atttgcaaga agaattagct gaacttcgtg aagaagcaaa
    24001 tgcgatgaag atgcagtggg aaacagaaaa agaagaagtc aattctgttt cagcaaaacg
    24061 tgctgaaatc gataaagcaa aacacgaatt agaagatgca gaaaataact atgatttaga
    24121 acgcgctgct gtattgcgtc atggtacgat acctcaatta gaaaaagaat tgaaggaatt
    24181 agaagctaaa gcaaaagaca gcgagattaa gatggttcaa gagtccgtta ccgaaaatga
    24241 gattgctcaa gtcgttggca gattaacggg tatccccgta acgaaacttg tagaaggtga
    24301 acgagaaaaa ctaattaaat tgaatgaaac acttcataaa cgagtgattg gtcaagatga
    24361 agcagttgat gcggtcagtg acgcggtaat tcgttcacgg gctggattgc aagacccaaa
    24421 tcgtcctcta ggttctttcc ttttcctagg accaactggt gtcggaaaaa ccgaattagc
    24481 gaaagcgtta gctgagaact tgtttgactc agaagatcat atggtacgga tcgatatgag
    24541 tgaatatatg gaaaaacatg ctgtgtctcg tctagtcgga gctcctccag gctatgtagg
    24601 ttacgaagaa ggtggtcaat taacggaagc tgtcagacga aatccttata caatcgtctt
    24661 gttggatgaa atcgaaaaag cgcatcccga tgtcttcaat atcttgttac aagtcctaga
    24721 tgacggacgc ttgactgatt caaaagggcg agtagtagat ttcaaaaata cggttctgat
    24781 catgaccagc aatatcggtt cacagttact gctggaaggc gtgactgctg atggaacgat
    24841 tccagaagca gtggctgaac aagtcaacac gctattacgt ggaaacttca aaccagaatt
    24901 cttgaaccga atcgacgaca cgattttatt tacaccattg agcctagaca acgtgaaagg
    24961 aatcgttgac aaaatggtgg cgcaattggc acaacgtctg gaacatcaag aaatcctgct
    25021 gacaatcagt gatgaagcca aaacgtggat tgctgaaaat gcttatgaac cagcatatgg
    25081 tgcaagacca ttgaaacgat ttatcactaa ggaagtagaa acaccactag ctaaagaaat
    25141 cgttgcagga catgttatgc ctaaatcaaa agtaacgatt actttacttg atggacaact
    25201 tcatttcaaa acagaagagc tagaagaaat cgtctaatct tccattgaga ctataaaaga
    25261 aaaaaggggt ctgacacaac tagtgtcaga cctccttttt tcaaataaac agtgttccag
    25321 aagtagtttc ttcgaaaaat aagccgaaat ttcttgaagc tgcccgcttc tgtcacaacc
    25381 tcttttatta ggtatttttc actttttcta atttataact ttcaaagtaa cgctcatctt
    25441 ttttactttg agaccatgca aacaatccca ttaaggcagc acctatcgta tttgtcagca
    25501 agtcgcccat cgtatccatc aatgctgctc gtccgacaaa cagtgtgcca tcagaagcag
    25561 caaaacgttg aagattcatg cctaagaact ggtcgcataa gaattcccag aactcccaaa
    25621 acacgccgca aagtccagca aacgcaaagc cgaataagag gaacaaccaa ggactggttt
    25681 ttgatatctc agcatctttc atcaagtaac caatcaatcc atagccaaga gctgtcagga
    25741 ccattggact caccgcatgt aagattttgt cccaaaaaga gatgatactg atcaaatgca
    25801 tccctgtgcc taaaaagaca gagatgaaca agaaaaacca gtaaaacaaa acgattgctg
    25861 ctggtatttt aagtttgaaa actttcaaaa tgacttgtgg catgaaaatc aatgcaatcc
    25921 ctaataaaga ttccacgata aatgtgattc cttgagaagt cgaatatttt tgtgtgataa
    25981 attcataaaa attatagcag acaacgaata caccaaaagc aagtaaagct atttgcacct
    26041 ttttgatttc tttaatcact gaaagtccct ccatctaaaa atctttttac ttctgaaagc
    26101 aactcaattt ctgaagaaac cacttttccg ttcaatttga tcaaacctat tgtatataag
    26161 ttcaggtagt gaaattgatt ttcagctgtt tcttgtagtg cagcgatttt tttcgggtgg
    26221 gttccgcctt gctgtcgagt atcagtatat aagccgatca ctgggatgcc tttcgcataa
    26281 gccacaccga tttccgatgc taccccggca tcgatcgtca gcccatctag taaagcaatc
    26341 attaaatcac tagccaacac tttttctgtg tcagcaagag caatcattcg actgtctgca
    26401 taagcagttt tgtcattgat tgcttcattt tcttgaggaa gatagatcga aagctcagaa
    26461 gagagctctc tgatttcttt aactaactgt tggttatata acaaatcact tttagcaaac
    26521 attggtgcgg caaaataaat tttcatctaa acatcatcct ttttatcaaa aataatcgct
    26581 aaagtaagct tccatgtttg gaatgatacg ctctagtgac ttcagtgttt cttcatctgt
    26641 ttctaaccgt tcgattcttg ccaaataaga agcacggcga taattcatca tcaagcaagt
    26701 caatgtttgg atatccagtc gaacggattt tcctataggt tcatttaaga cacggacttg
    26761 tccctgttca tcccatgtta aagcgaagac accattattc caaggagcga caggatcttc
    26821 aatgatgaaa tgaaaagcat cagctgttcc aacaaacgga aaccgttgaa ggaattcttt
    26881 tacatcaacg atccgagcca taaaatatgg ttcgatctgc tctttgatct gactatcttc
    26941 aatcaaaaat gctaaaggct catttttata aatatctcca tgtacccagt agatcatgga
    27001 aaaatgtgcg gaaataaagt tccataagcc atttcgagct tcttgatcaa gatagaacat
    27061 ttcttttacg tgaaatattt cttcagctac ccagtagaac agaacacctc ttggttcatg
    27121 gttgctatcg tagtagacag cggctgtgcg ctcatcttca ttttcgaaac gccaatactc
    27181 ttcccaatga aaatcactgc ggaataaagc accatggttt tgttgggcaa atttgtcata
    27241 aacggaaaat acgtctggat gatcaactgg taatcgctca accataccac ttactgggat
    27301 ttgtttaggt agctgtgtat ctctgatttt aaaggaaagt ttatcagaca tgatttccca
    27361 tcctttacgc cgatagtagg ggatactgta aggatacaaa taagagatcc attgcttatt
    27421 cttgcgcata ttatccaatg caacgataat tagatcttgc atcaatccat ggttagcata
    27481 ttctggataa gttccaactc cagtcacacc acccatttta taacgtgcac cgtgaatatt
    27541 gacttcacaa ggatagatag cgatttgaga gatcagttta ttttcgtgga accatccgaa
    27601 cactttcgat acttccaaaa tcggtttttt ggaacggata aaagctcgtt tattctcaaa
    27661 cccgctttcc tcgatgtctg cttcagtcac ttggaacaca taggaaagca attcattgaa
    27721 ttgatcgata tgttcttgag tgaccggttt taattcaaga ttttctctaa aatctttttg
    27781 atccattttt tcatctccat ttattagctt gataacagta tagcaaaatt ctacaaaaaa
    27841 aacttctata gctgactaat taaaaactct ttaaattact gcttttcgtt ggtataatgg
    27901 aaagtgctga ttatatgaaa agaggagacg ctatggatat aaagaaaatg aaagaacgcc
    27961 aagagcagat tcgtaatttt tcgatcatcg ctcatatcga ccatgggaaa tctacattgg
    28021 ccgatcgtat tttagaaatg acgcacactg tgacgtctcg cgaaatgcaa gatcagctgt
    28081 tagactcgat ggatttggaa cgtgaacgtg gaatcacgat taaattaaat gctgtagaac
    28141 tcaattacac agcgaaaaat ggagaaacat atatctttca cttgattgac accccagggc
    28201 acgtcgactt cacctatgaa gtctctcgaa gccttgctgc ctgtgagggt gccgttttag
    28261 tggtcgatgc agcacaagga atcgaagcac aaacattggc caatgtttat ttagcgttag
    28321 acaatgattt ggagatcctg ccagtcatca ataaaatcga cctacccgca gcagatcccg
    28381 aacgtgtccg tcaagaaatc gaagacgtaa tcggaatcga tgctagtgaa gctgtattag
    28441 ccagtgccaa atcagggatt gggattgaag atattctcga gcagatcgtg gaatatgttc
    28501 ctgcaccaag tggagactta gatgcaccgc taaaagcttt gatctttgac tctatttatg
    28561 atagttaccg cggtgttgtt ctaaatgtcc ggatcacgga cggtatggtc aaacccggag
    28621 acaaaatcaa actgatgagt aatggcaaaa catttgatgt tacagaggtc ggggtcttct
    28681 cgccaaaagc agttgctaga gactttttga tggtcggtga tgtcggttat atcacagcaa
    28741 gtatcaaaac tgtccaagat acacgagtag gggatacagt aacgttagca gacaatcctg
    28801 ccgctgaagc tttaccaggt taccgaaaaa tgaatccgat ggtttattgt ggtctttatc
    28861 caattgatac ttctcgttac aacgatttgc gggaagcttt agaaaaactg caattgaatg
    28921 atgctgcttt acaattcgaa ccagaaacct ctcaagcatt aggttttggg ttccgctgtg
    28981 gattcctcgg acttcttcat atggatgtcg tccaagaacg cttggaacgg gaattcaatc
    29041 tagaattgat cacgactgca ccatccgtta tttaccatgt taataaaacg gatggtacga
    29101 ctgctacagt agataacccg gcagattttc ctgaacctgt aacgattcag gatgtagaag
    29161 aaccattcgt caaagcacag atcatggtcc caaatgattt tgtcggtgca gtaatggaac
    29221 tttctcaacg aaaacgggga gaattcatta cgatggatta tttagatgat taccgagtaa
    29281 atgtcgtcta taatattccg ctttctgaaa ttgtctttga ctttttcgac aaattgaaat
    29341 caagcacgaa aggctatgcc tcattggatt atgaaatgtc tggttatcaa aaaagcaagt
    29401 tagtgaagat ggacatcttg ctgaatggag aaaaagtcga tgcattaagc tttatcgttc
    29461 accgagactt tgcctatgaa cgtggaaaag caatcgttga aaaactgaaa aaactaatcc
    29521 cacgccaaca atttgaggtg ccaatccaag cagctattgg acaaaaaatc gttgcccgtt
    29581 cagacatcaa agccttgcgt aaaaacgtct tggctaaatg ttatggtgga gatgtttctc
    29641 gtaaacgtaa actcttagag aaacaaaaag aagggaagaa gcggatgaaa caaatcggct
    29701 ccgtcgaagt tccgcaagaa gccttcatgg ccgttctgaa aatggacgag gacgaaccga
    29761 agaaatagca agggtttgtt tgaaaacagt gtaaaaaata aaccatgttt acatggattt
    29821 gccaccggtt tgccaccggt ggtttttttc ggtggcaaaa atcaagaatt ctttctaaga
    29881 ttttccatga tatcgaccgt ttcatttctc atcttatctg ttacatgcga gtaagtatcc
    29941 atcgtaattg aaattctgct gtggcccaat cgttcagaga tttctttcat ttttgcgcca
    30001 ttttcgagaa gaagtgtagc atgagtatgt ctgagagaat ggaagttaaa agagagggag
    30061 agtgcattcg atatcctcct agtattccat ttcactacac ttggcgtaac tagctcaccg
    30121 tcttccttcg tacatactgc atttgaatca atgtagagct ttccatactt cattcgattt
    30181 tctaattgtt gtttcttatg ttttttcaga attgctagca aggtttgtcc aataaaaatc
    30241 gttcgattgg aactgcttgt ctttggcgta ccatataccc atgcgccgtc attctttacc
    30301 atttgtttct ctacagtaat tgttccattt gaaaaatcga catcatccca cgtcagacca
    30361 caaacttcgc caacgcgcat tcccgtataa aatccaatat tcaaaggaat atagaaagga
    30421 tgtccttcag gagtaatttc tagcatatga tcaaagtcct caagagaaat gatttttaga
    30481 tcttttttag tcgttggtcg tgcttcatat tttggtattt ttacatacag cataggattt
    30541 tgcttgatta atccccaagg ataaaccgcc atattcagcg cattcttaag gacagagtga
    30601 gtaatagtca ttgttttctt cgagtaaccc tttttaaatt cagcattgat gaaattttgt
    30661 aaaagagcag gggagagatc cgtaagtttt ttctttccta aataaccgtt tatatgattt
    30721 ttgatggtaa atcggtagtt ttcataggta ttgtatttta gatttagttt aacgtattcc
    30781 tccatccaaa aatcaaggta ttgttttact cgagtatccg tacctaaaaa gtattgtcct
    30841 gtttcgtcaa tatctgataa aacttttcgt aaagcagctt cggcctctgg tcgggtgtct
    30901 ccgccaactt tctccacttt ttttcttgag ccatcatcat tgatatcttc gccgtttgta
    30961 taattaagcg agacaaataa aaaagccatt tatgttacac tc
//
LOCUS       DACEPD010000024        30939 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.024, whole genome shotgun sequence.
ACCESSION   DACEPD010000024 DACEPD010000000
VERSION     DACEPD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.024"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(2..1990)
                     /gene="treP"
                     /locus_tag="IW797_001077"
     CDS             complement(2..1990)
                     /gene="treP"
                     /locus_tag="IW797_001077"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ1223864.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            2213..2926
                     /gene="treR"
                     /locus_tag="IW797_001078"
     CDS             2213..2926
                     /gene="treR"
                     /locus_tag="IW797_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ1223865.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            3003..3449
                     /gene="msrB"
                     /locus_tag="IW797_001079"
     CDS             3003..3449
                     /gene="msrB"
                     /locus_tag="IW797_001079"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ1223866.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            3619..4221
                     /gene="ruvA"
                     /locus_tag="IW797_001080"
     CDS             3619..4221
                     /gene="ruvA"
                     /locus_tag="IW797_001080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ1223867.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            4234..5235
                     /gene="ruvB"
                     /locus_tag="IW797_001081"
     CDS             4234..5235
                     /gene="ruvB"
                     /locus_tag="IW797_001081"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ1223868.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            5264..5842
                     /locus_tag="IW797_001082"
     CDS             5264..5842
                     /locus_tag="IW797_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1223869.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            5894..6376
                     /locus_tag="IW797_001083"
     CDS             5894..6376
                     /locus_tag="IW797_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ1223870.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            6493..6867
                     /locus_tag="IW797_001084"
     CDS             6493..6867
                     /locus_tag="IW797_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223871.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(7667..9019)
                     /gene="brnQ"
                     /locus_tag="IW797_001085"
     CDS             complement(7667..9019)
                     /gene="brnQ"
                     /locus_tag="IW797_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1223872.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            9166..9756
                     /locus_tag="IW797_001086"
     CDS             9166..9756
                     /locus_tag="IW797_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ1223873.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(9884..11233)
                     /locus_tag="IW797_001087"
     CDS             complement(9884..11233)
                     /locus_tag="IW797_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1223874.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(11401..12141)
                     /locus_tag="IW797_001088"
     CDS             complement(11401..12141)
                     /locus_tag="IW797_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1223875.1"
                     /translation="MNEKIIEINHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(12154..13746)
                     /locus_tag="IW797_001089"
     CDS             complement(12154..13746)
                     /locus_tag="IW797_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1223876.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(14314..14610)
                     /locus_tag="IW797_001090"
     CDS             complement(14314..14610)
                     /locus_tag="IW797_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223877.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            14786..15511
                     /locus_tag="IW797_001091"
     CDS             14786..15511
                     /locus_tag="IW797_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ1223878.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            15504..16346
                     /locus_tag="IW797_001092"
     CDS             15504..16346
                     /locus_tag="IW797_001092"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ1223879.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            16349..16870
                     /locus_tag="IW797_001093"
     CDS             16349..16870
                     /locus_tag="IW797_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ1223880.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(17113..17676)
                     /locus_tag="IW797_001094"
     CDS             complement(17113..17676)
                     /locus_tag="IW797_001094"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1223881.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            17927..18196
                     /gene="rpsO"
                     /locus_tag="IW797_001095"
     CDS             17927..18196
                     /gene="rpsO"
                     /locus_tag="IW797_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ1223882.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            18384..20510
                     /gene="pnp"
                     /locus_tag="IW797_001096"
     CDS             18384..20510
                     /gene="pnp"
                     /locus_tag="IW797_001096"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ1223883.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLFKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            21020..21286
                     /locus_tag="IW797_001097"
     CDS             21020..21286
                     /locus_tag="IW797_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1223884.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            21430..23421
                     /locus_tag="IW797_001098"
     CDS             21430..23421
                     /locus_tag="IW797_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ1223885.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            23775..25076
                     /locus_tag="IW797_001099"
     CDS             23775..25076
                     /locus_tag="IW797_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ1223886.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(25206..26576)
                     /locus_tag="IW797_001100"
     CDS             complement(25206..26576)
                     /locus_tag="IW797_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ1223887.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(26758..27081)
                     /locus_tag="IW797_001101"
     CDS             complement(26758..27081)
                     /locus_tag="IW797_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1223888.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(27407..28015)
                     /locus_tag="IW797_001102"
     CDS             complement(27407..28015)
                     /locus_tag="IW797_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ1223889.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            28345..29061
                     /locus_tag="IW797_001103"
     CDS             28345..29061
                     /locus_tag="IW797_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ1223890.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            29074..29769
                     /locus_tag="IW797_001104"
     CDS             29074..29769
                     /locus_tag="IW797_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ1223891.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHAELYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            29853..30482
                     /gene="udk"
                     /locus_tag="IW797_001105"
     CDS             29853..30482
                     /gene="udk"
                     /locus_tag="IW797_001105"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ1223892.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        10016 a         5841 c         6297 g         8785 t
ORIGIN      
        1 actaagattt tacttcagct ttaccgactg cttcgcccgg gcgaacaagt cctgattggt
       61 caagagaaag tcctgcaact ttttcattat tggtcaaagc aacaataatg tcagttttct
      121 tgccagcttg ttctatttct ggcaaatcca tttcagcaat cagggtctct ggtgtgacaa
      181 ggtatccttc tttcacgaat acattgaaag gaacaccctt tagttcaaca gtatctaatc
      241 ccatgtgaac aagtacttcc agcccctcat tagaaagaat accaatggca tgttttgttt
      301 caaagacact cgtcacttta ccgtttactg gtgcatagac tttgccgttt gacggttcga
      361 cagcataacc gtcacccatc attttttgtg agaaaactgg gtcatttact tcagttattt
      421 ccttgatggt ccctgcagcg actgcgaata atgtttcttt tgttgcaact gccgtaccag
      481 ttgctgaaac aggttcgaaa gactgtggcg ttgctgttgc gccatttgct gtttgaagtt
      541 gtacatcagc agcattttca ggaacagcag gatcaatctt attcaaaatt ccctgacgtc
      601 taaagatgat cgtcaagcca aatggaatga tgacagcaat gatcattgcg atgatgaaag
      661 gtacccatgt ttctgcttgg atagctagta ttcctggcaa accgcctact ccgatcgcgt
      721 tagctcgaac acccatcaag ttagcaaaca tgccagctaa tccagagccg accattgcag
      781 cgacgaaagg atagacgtat ttcaaattga ttccgaacat agcaggttca gttacgccta
      841 agtaacaaga aatcattgct ggaatcgaga tttgttcttc ttttttattc ccacgatgta
      901 agaagatgat ggctaagact gcagagcctt gggcgatatt tgataaagca atcatcggcc
      961 ataaattcgt tgatccgaaa tctgcaatca gctgcatatc gatggcattg gtcatatggt
     1021 ggagaccagt aatcactaaa ggagcataca ggaatccgaa tacggcacta aacaaccagc
     1081 tgatagaaga cgtcaatcca gtatttacga tatttgagat ccagcttccg actgtccagc
     1141 cgataggacc taaaataacg tgtgcagcta aaacagttgg taacaatgaa aacaatggaa
     1201 caaaaatcat cgagatcgac tgagggatat atttgcggaa gaagatttct agatacacta
     1261 acataaaccc tgccagcatg gctggaataa cttgcgcttg ataaccaatc atctggactt
     1321 gagcaaaacc aaaatcccaa aatggaatat ctgctgcggc tgtggaagct acactataag
     1381 catttaataa ttgcggagaa actaacgtaa ttcctaaaac gattcctaat atttgtgtag
     1441 tacccatctt ttttgcaata ctccaagtga tacctactgg aagaaaatgg aaaattgctt
     1501 cacccggtaa ccacaaaaag gcattgacac cattccaaaa cttcgagtgt tcaacgattg
     1561 ttccaccaag actttcaaac tggatacctt caagtacatt tctgaatcca aggatcaatc
     1621 caccgacaat gattgcagga atcagtggtg taaagatttc tgctaaaacg gcaatagcac
     1681 gttgaacggg atgaaggttt tgttttgcag ctgcttttcc ttgttctttg gaaacccctt
     1741 caactccaga tactgcaacg aagtcgttat aaaaagtaga aacatcgttt cctatgatga
     1801 cttggaactg tcctgcattc gtgaacatcc cttttacgct aggtatatct tcgatcgctt
     1861 tttcgtccgc ttttcctgga tcattcagga caaaacgcat acgagtcgca caatgactaa
     1921 ctgcagcaat attttctttt ccgccgatat catgtaacag cttctcggca tctgcttgat
     1981 attttgccat atcaataaac ccctttctct taacttgtct atgcaagttg tttcttcatt
     2041 tactatactt tttttcggag agagttgcaa gcgttttatt aaaaagtacg agagaaaatg
     2101 atatcgtttt aaaaagctta aaataagctt ttcttaaaaa taggaacatt gaaatacaaa
     2161 aagagctgta gtatagtgaa gaaatgatgg aagaacgagt gagggaaaaa acatgaataa
     2221 gttcaatgaa atttttttag atttggaaca gaaaatttta gcgaaagaat atcctccgca
     2281 ttcgctttta cccagtgaga accaattgat caaaatttat ggtgtttcac gagaaacaat
     2341 tagaaaagcg ctgaatcttt tgactacgga aggttatatt caaaaaaaac aaggaaaagg
     2401 ttcgatcgta ctggatcgga atcgcttcga ctttcctatt tctggattga ctagctataa
     2461 agagctacag gagacccaac gtattccaag tgaaacgatc atccatacgt tagaagaaac
     2521 agaagtgaca aaagcattga acgagatcac tggctgggag atcggtgcgc ctgtttggca
     2581 tttgatacgc gaaagaaaaa tcaatggcga agtagttata ttagacaccg attatctatt
     2641 aaaggaaatc gttcctattt taactccagc acaagcacaa ggatcgatct atgaatattt
     2701 tgaaaatgag ctttctttga cgattgctta cgcgcaaaag gagatcacag tagaagaagt
     2761 aacggaaatc atgaaaacag cgatggatct tcacgatgac acttatgcag tagtagtcag
     2821 aagcctcgtt catctggaag atacccgctg tttcgagtat tctgagtcgg ttcaccgatt
     2881 agataaattc cgtttcgttg agtttgcccg acgccgaaaa gtgtaaatag ttcaaaagaa
     2941 agcaaattgt tggcatctgt ccactttggc gctatgatag gatcacaaag ggggcatttt
     3001 taatggataa accaataaga ccagaaaaag aagaactgaa agaaaagtta tcacccatgg
     3061 cttatgcagt cacacaagaa aatgcaacag aacgaccatt tagcgggaaa tatgatgatt
     3121 tttacgaaaa ggggatctat gtagatatcg tcagcggtga accgttattc agctctgcag
     3181 aaaaatatga tgcaggttgt ggctggccat cattcagcaa accaataacg aaaagacaag
     3241 tgcgcgaaaa agccgatttt tctcatggca tgcatcgaat agaagttcga agcaaagaag
     3301 cagattctca tctcggacat gtgtttaccg atggaccagt agatcaaggt ggtctacgtt
     3361 actgcatcaa ttctgcctca ctgaaattca ttccatatga aaagatggat gaattaggtt
     3421 atggggaatt caaatcactt gtagaataat ggaaatacac aaagagcggt acaatacggg
     3481 gatttactcg atcgtccgct ctttttttat tcaagcgatt gctatttgaa agaaacgctc
     3541 cccagactcc ttttttcaca aggtgaaatg tgctacaata aagggaacgt atgtgtgcaa
     3601 aagaaagagg actagaatat gtatgaatat ctgacaggtg tggttacctt tatcaacccc
     3661 tattacatag tagtagaaac caatggtatc ggctaccaga tcgcactagg aaatccttac
     3721 cgctacagta gcaaactaaa tcaaaaaata aaaatttatg tccatcaggt aatacgtgaa
     3781 gatgcgcatc ttctgtatgg atttgattcg ctagaagaaa agcaactgtt tttgcgatta
     3841 gtaagtgttt caggaatcgg tccgaaaagt gcccttgcaa tcatggcaag cgaagaccac
     3901 tccggtttga ttcaagcagt agaatccagt gatgtcactt atttgacgaa atttccagga
     3961 gtagggaaaa agacagcaca acaaatgatc ttggatctaa aaggaaaatt cggtgagctg
     4021 agtatcgata caccttttag tctatttgat gaagcaaaca caaaagacgc tacagctcta
     4081 tctgaagcaa tggaagctct ctcggctctt ggttacagtg atcgagaaat caaacgtgta
     4141 gaaaaacaat taaaagaagt agataatcag acaacggatg agtatctacg ccaagcactc
     4201 aagctaatga tgaaaaaata aggaggagac caaatgacag atgaatactt gttatcacca
     4261 gaatctggcg aaaatgaact gagtttggaa aaatctcttc gaccacagct gctttctcaa
     4321 tatatcggac agagaaaagt caaacaagaa cttcagattt acatcgaagc tgccagaaat
     4381 cgggaagaac ctttagatca tgttctgttg tacgggcctc ctggattagg aaaaacgacg
     4441 atggccatgg tcattgctaa tgagatgacc gtaaatattc gtacgacgag tggccctgct
     4501 atcgaacgtc cgggtgactt agtagcaatc ttgaatgaac tggaagcagg ggatgtcctg
     4561 tttatcgatg agatccatcg attacctaga gtggtagaag agatgcttta ttcagctatg
     4621 gaagatttct atgtagatat catggtagga caagggccaa ccgcacatcc ggttcatttc
     4681 cctcttcctc cctttacttt gattggcgcg acgacacggg caggtatgct ttctgcacct
     4741 ttacgagatc gatttggcat catttctcat atggaatatt atgacgaaca ggatctgcag
     4801 gagatcgttt tacgttcttc ggatattttt caaacagaga tcattgaaga aggggcattg
     4861 gaaattgctc gccgatcacg aggaacgcct agaatcgcta atcgcttgct caaacgggtt
     4921 cgtgattttg cgcaagtcca aggagacggg aaaatcgatc gtcctatagc ggatcaggca
     4981 cttactttgc tacaggtcga tcaggctgga ttggattatg ttgaccagaa attgttgcga
     5041 acaatgatcg agttatatgg cggcggacca gtagggttaa gtacgctttc agtaaacata
     5101 ggagaagagc gtgagacggt agaagatatg tatgaaccgt atctgatcca aaaaggattt
     5161 ttaaagcgta cacctagagg gcgaattgct actgcttatg cctatgaaca ttttggttac
     5221 gactatcata tatgattttt gcttttagat tggaggaaaa tgaatgagtg atacaacacg
     5281 tacgaaacaa ttgatcaaaa acgtattgat tgatttgtgc caaacgcaat cttttcgaaa
     5341 aatcagcgtc caaaaaatcg caaatgaagc aaaaatcaat cgacaaacat tttattatca
     5401 ttttacagat aaatacgatt tgcttcgttg ggtctattgt gaggatgcat tgcattattt
     5461 agatccacaa tgtctttcac ttgataattg ggaagagcaa gcattgaaag tgctgaaagc
     5521 aattgctgag aagaaagaat tttatgcaaa tactgtgctt tctgacccag aaattttaca
     5581 aaaagagttt tctcaactga tccaaaaatc atttatcaag atttttgaac aaatggatga
     5641 agaaagacaa ctttctgcaa gagacaaact attttatgct cgtttttttt cttacggttg
     5701 cagtggtgta ttagtcaatt ggatcacgaa tgaatatgaa gaaacaccga tggaaatcgc
     5761 tatgcagctt ttccgtttag ccaaggatac ggaattatat tcttatcgtc tttatgcaaa
     5821 agaagagttg gaagaacagt aatagaaaaa ttttttgttg gaaaagcaat aatcgctaaa
     5881 aggagatggg gaaatgacaa aaattttatt tgtttgtttg ggaaatattt gccgttcacc
     5941 gatggcagaa ggcttgctga gaaaacgaat agcaatagaa ggaagggaag cagaattttt
     6001 tgtggattct gctgctacaa gcacgtatga agttggaaag acaccgcacc ctggaacgaa
     6061 aaaaatatta aaccaagaaa aagtagatat gacgaatatg gttgctcggc aaatcacacc
     6121 ccacgatttt gaaacttttg attggatcat tggcatggat caagaaaatg tagaagaact
     6181 gaaacgaaga gcaccacgct cagcgcaggg gaaaatccat ttgtttttaa gtagcattcc
     6241 tggaaaagaa aaagaaaatg ttcccgaccc atactatacc aataactttg atgagacata
     6301 tcacttgatc acagaaggcc tgaatcattg gtggaatatt tggattgacg aaaataaaat
     6361 aacaaacaat ttgtaaagtt gacgctttct ttttaaggcg atgttagagc atttccttgt
     6421 ttttttgtaa aatgttaggt aaaataaaat taattaagta acactttctt agctaaagga
     6481 ggagtgcatt cgatgaacga agagacagta tcgccaatga agaagcttac gaagttagtt
     6541 actcatactt cgtatcaact ttttgattct tttgtaaatg acactgaaga acatgctaag
     6601 aatgtacgat tgcaaggaaa gtttattctt gagctgcaaa aagctgctac tgaacatagt
     6661 ctagttatct tacaagtaag agcgaatcgt ttgagtagca agtatgaaac gatctcaggc
     6721 tgggtggcga caaaaacggt cgaaaaagac cgagtgatga tccgcctaca aaacgaagag
     6781 cagcagatcc ggatggttcc tgtagaacaa attgaaaaaa taacaatcct ttcaccagca
     6841 ggggaacagg aacgattatc aaaataattt ataccagata gaaaagcacg tatgagcttg
     6901 taccatcaat cattactaat caaaaataac cgaatgcttt aagctgatat cagtaaaacg
     6961 gaacgtttta ggatgagtat cacagcatga cagcattggg ttgattagtt gaatcgattg
     7021 gatggtacaa gtttttttga taaaaaaagg tcctgctagc cggggagcta gcaggataag
     7081 gagttaaaaa tgaaaaagtg ttgttagggt tgtttgttgg tatgcttata taatagtcac
     7141 tagttgtgaa tattttgtga cgaaagtttt cgaaagttgt taagtttttt tgcgaaaact
     7201 atttttatat catatatatg tctaaaatct atattataaa agaaatgttg tttgatcagc
     7261 ttctcttcgg tattagttca aaacaaaacg gcaaagtata tggagctact ttgccgtttt
     7321 gtttctctaa tactcaggtc agtttataga cgctcaataa acggtgttca aaagccagtt
     7381 tctcggtatt aagtcaaatt tatgcaaaaa tgtgagaagc cattttcaca aaatatttct
     7441 taatacttga aactaagcgg ctttttcacg acctctcaat aaacggtgtt caaaagtcag
     7501 tttctcggta ttaagtcaaa tttatgcaaa aatgtgagaa gccattttca aaaaatattt
     7561 cttaatactc gaaactgagc ggctttttca cgacctctca ataaacagtg ttcaagagac
     7621 agcctatcgg caataaggct gaacgtctct ttcacaacct cttttatcaa tctgctactt
     7681 cttctttctt agcgaaactc caaatcagtc cgataacaaa accgataact gctggtacga
     7741 tccaccccat ccccaatttg aaaaacggca agtagctttc cgcaacatat aaaagatttt
     7801 gaacaatagg tgtttgtttg atagtttctg ggcaagcgtt caacccatca aaaatcgaag
     7861 caatcaatgt aaaataagta gtcattcgat aaacagccgt acgctgctta aacaaaggac
     7921 caactaacac tagtaaaatc agtgtcattg ctaatggata gatgaacatc aaaacaggca
     7981 acgaaaattg aatgatattc gtcaagccta cgtttgccgc taaacaagga aggatactag
     8041 caagtgcaac aaagaatgcg taatttcttt gcggaaacaa atctgtgaat gtttctgcaa
     8101 aagctgtaat caaaccaata ccggtcttca agcaagctaa tataacgata aatgctaata
     8161 agatactgcc ataagtacct aaataatgat cagcaatctg agccagcgtg atcccgccat
     8221 tttcacttag agcaaagcta cctaagctca tagcacctaa ataagcgagc aaagtataaa
     8281 tgattcccat caatataatg ctgatcgcac cagatttgat cgtgtcttta gcgatttcag
     8341 caggttttgt tacacccatg ttttggattg ttgtgacaat gatgatccca aatgctaaag
     8401 cagcaagcgc atctaatgtg ttatatcctt gagtaaaccc tgtgaaaaaa gcatgctctt
     8461 gataatttgg ctgaataggc gcttgattta cagaacctag tggattcaaa aaagcaagga
     8521 gaagcaaagc acctaataaa accaaaaatg caggattcag gaactttcca acataatcta
     8581 atatctttgt cggttttctt gaaaaccacc aagccagcac aaaaaataca atactgaaaa
     8641 ttgctagata tagcgtctgt ctgctaggat cgataaatgg tgctaaacca atctcgaaag
     8701 atgtggttgc aaggcgaggt aaagcaaaaa acggtccgat aactaagtaa agcaagatgg
     8761 taaaaaccat cgcatatgtt tgatttatcc gttttgctaa ttcatataca cctgagcttt
     8821 tcgatacacc aatggcaatc acccctaaga agggaagacc gataccggta accaagaaac
     8881 ctagattcgc ccaaaaggta gcagcgcctg cttcttgacc caaatgaacg ggaaagataa
     8941 gatttccagc accaaaaaat agaccgaaaa gcatggagcc tataaaaaga tagtctttaa
     9001 atgataattt ttttgtcata cataactcct ttacggtttt tttaaaagta acaaaatttc
     9061 gaaaaatatg caacaatttt cagaaaattt cctatataaa acgacggtta tttaataacg
     9121 taagtgataa aataaaagcg ctgaatcatc tgaggaggaa tcatcgtgac aaagtcgaga
     9181 ttagaagcat ttactgatgg agtggtagcc attgttttga gagttcttgt attagagatc
     9241 aagatcccta atacatctag ctttcattct ttatgggtca tccgaaatac attattagct
     9301 tataccatca gctttatatt tgttgcggtg atctgggtga accatcatcg tatatttcag
     9361 atggcagaac gaattaacta ccgagtggtc tggtcgaata tctttttgct cttttggctg
     9421 actttatgtc cttctgttac tagttgggtc gggcgtaacc ctgaagcttt ttggcctgag
     9481 atggcttatg tcttagtata cacgatgtgg agtttttctt ttggtatctt gatgaggcaa
     9541 atcatgaaag caaataaacc agattcccat gtcgtgagag ttttgtcaaa ggaccgtcga
     9601 agtcttatct ccatgatgat taacttatgc ttgattgccg gtgtcttttt tgtaccgttg
     9661 atcgggttat tcggacgttt ttttgtatct ggtatttgga ttttttccta taaaaaagca
     9721 gatgcctatt atcagcggtt gtttccgggg aaatagaaaa gaggctggga cagaagcttt
     9781 taactccaag aaataagccg aaatttcaca aaaatttaag aagcaatttt cgtaaattcc
     9841 ttcttatttc tcgaagctga ccacttctgt cacaactact ttcttacttc ttacgttcat
     9901 atttcatatg atagattagt aatagaagca agaaccacac aggtgttagt aataaagcga
     9961 ttctcgtatc ttttcctagg aacaagacga cacacacaaa ggccatgtaa agaaatacca
    10021 gataattact aaatgggtac aacggcattt taaatgaatg agcttttttg ccgaggatac
    10081 ttttacgata tttcatgtgc gtgtagacaa gcatactcca gataaacaag aaacaagtag
    10141 tggcaatact gcttactagt gtgaatactt cactaggcat cacgtaattc aagataacag
    10201 aaatgaaaat gacgatagta gaaaatgtca aagcattccc tggcacatga tgtgttgtta
    10261 actttttgaa ttttttaggg gcactgcctt cttgcgctaa agaacgaagc atacgtcctg
    10321 tactatatat tgcactgttg caggcagaag ctgctgcact taatacgact aaattgatca
    10381 aggaagcagc aatcgtgatc ccaatttccg aaaatacttc tacaaatgga ctgttatctg
    10441 gattcaagct gttccatgga tagattgcca taatgacaaa aagagatcct aagtaaaata
    10501 agatgatcct gataggaata ttattgatag cttccggcaa cactttttca ggattttctg
    10561 tttctccagc gactaatcca actaattcta tcccagcaaa agcaaaggtt accatttgga
    10621 aagctaagat aaatcctttt gcaccagttg gaaagaatcc gccatgagac catagattcg
    10681 tgatactggc tgtgccaata tctgtcgtat agtgggtgag gatcatataa gctcctacga
    10741 ggataaaggc aatgatcgct acaactttga tcaatgcaaa ccaaaactct aactcaccaa
    10801 acaatgagac agccaccata ttcaagcaaa gaagaagacc taatgcaatc agttcaggaa
    10861 gccactgagg aacaccaggc gcccagtagc gtacatacat tccgatggct gttaaatcgg
    10921 ccattgcaat cgatacccag cagaaccaat acgtccagcc agtgatgaaa gccgcttttt
    10981 tgccaagata ttctgcaaca aaatccagaa aagaatggtt attggtgttt gataacagaa
    11041 gttcgcctaa cgcacgcata atgaaaaaac agatgcatcc agtaataaga taggcgagca
    11101 atatcgaagg gccagctagc tggatagact ttccggaacc taaaaacaac cccgttccaa
    11161 tcgctccgcc aatagaaatc agttggacat gtctattttt cagtccacgc tgcagttctt
    11221 gctcttcatt catttttaaa tcatcctaac taaaaaagtt taattacttc catattaaaa
    11281 gagttttctg aaaaacacaa ctaagattcc aaaattttca gaaatttacc ttttatgagt
    11341 aattcataaa aaagaactgc ggtaaacacc gcagctcttt gtcccaatca gctattgatc
    11401 ctaagcattt aaaatacgtg aaaggaattc ttttgtacgt tcttctttcg gatggacaaa
    11461 gatatcttct ggtgtacctt cttctgcaat cacaccttta tccataaaaa tgacacggtc
    11521 agatacatct tttgcaaatt ccatctcatg cgtcacgata agcattgtca agccagtatg
    11581 tgctaagtct ttgatcgtat taagcacttc gccaaccatt tcagggtcta acgccgaagt
    11641 aggttcgtcg aatagcaata catctggatt cattgataat gcccgagcaa ttgcgacacg
    11701 ctgtttctgt ccaccagata attgtgctgg tttggcttcg atataacgat ccataccaac
    11761 tttttccaga ttttcgattg ctactttttt ggcagtttct ttatcacgtt tcaggacagt
    11821 tgtttgacca gacatacagt tttccaagac attcatattg ttgaacaggt tgaaggattg
    11881 gaaaaccata cctagattcg ttcgatattt aggtaaatta tatttaggag ctaatacatt
    11941 ttctccatta taaatgatct cgccgcctgt tggtttttcc agtaaattga tacagcgcaa
    12001 gaaagtagat ttaccagaac cggaagaacc gatgatcgtc acaacttcac ctttattgac
    12061 tgttacgctg atatctttca atacttcatt ttcaccgaag ctttttctta gatgattgat
    12121 ttcaataatt ttttcattca tgattcttcc tccttattcc tgcaaatttg aattgtcgat
    12181 ttcttcaagc ttggtataag ctgaaggacc atcgattttc ttttctaccg cccgcaggat
    12241 tctggtgatc gtaaaggtca tgactaagta gatgatcccg acgatcgtaa atgtttggaa
    12301 gaattggaaa ttcgcaccgg aagcagagtt tccttggaag aacaaatcag ctacgccgat
    12361 tacacttaaa actgctgtat ctttgatatt gataacaaac tcatttcctg ttgctggcaa
    12421 gatattccgt aaaacctgtg gaataacaac ttttctcatc gtttggccat gggtcatacc
    12481 aatggcttgc gcagcttcga actgcccttc atctactgca aaaatccccc cacgaacgat
    12541 ctctgtcatg taagctccag tattgagaga gacaataaac aaagcggcaa tcgttcggtt
    12601 caatgaaatg ccaaaagcga gtgccagacc gtagaagatg accattgctt gtaccatcat
    12661 cggtgttccg cggaatactt cgacatagat agaaagaagt gcatttccga ttttttggaa
    12721 gaagcgagtc agtgcatttc ctgattcagg taccgaacgg aagacaccaa tcagtaatcc
    12781 aagtgtagta ccgacaactg tcccgatcaa tgcgatgaac aaggtaagtc cagcacctcg
    12841 taagaacata ttgccgtatt gtttccaaat cgtagtgaaa tcagaaatga tgccaggttt
    12901 ttcttcacta tcagttgcag caggctgatc tttgactgct tggtccataa ttttggttcg
    12961 ttcatcgtta gagatgccag aaagaatctg gtttactttt tggatatccg ggtcgccttt
    13021 acgcatacca acagaaatct gaacatcttc cgggtttgtc tggaaaccat ctgcaccgct
    13081 gaattcaagc atttccaagt ctttgttcac actagttgcc gttacacctt ccggacgttc
    13141 gctgacataa ccatcgatca ttccagaagc taaagctgtt ctcattgctg agaaattatc
    13201 cattgcttgt tgtttctgca catctggtat ctgatcgatc acagtatagt ggaatgtatt
    13261 taactgagca gtgatctttg ctccagaaag atctgctaaa ctttttgctt ttgcatattt
    13321 gctatctttt tgaactacta ctactaattg tgattcgtaa taaggatcgg taaaatcaat
    13381 ttcttttcgt cgttcagcag tagggctcat tccggcgatg atcgcgtcga tcttgcctga
    13441 ttgaagggca ggagccagac catcccattt cgtttggacg atgactaatt ttttccctaa
    13501 accatctgct acttttttag cgatttgaac atcataaccg ccggcgtaac tattttggcc
    13561 ttgaattggc aaggcattat tctcatctgt ttgttgagac cagttgaagg gtgcataccc
    13621 agcctccata cctacgcgaa actcatcgct cggtgttttt tcatctgcat ttgccagaga
    13681 tcccacgctg aatagtgaga agacaaaagc aaatagtacg gcaactgaaa ccatattttt
    13741 tttcattttt ttctcccttt ctgattatat gatacacgag cggtctagat gaaatgcggt
    13801 aaagctttat ctgaaaaaat aaaaagcgac cgatttatag atataaaaag gccgctaaaa
    13861 ttccataagg tgataaacct ctcacaaaac atagcgcaac ttagcatcac tgctaagaca
    13921 gtccgcaagc tcttaacttg cgtcccaaca tacactccaa gcaaaaaagt gcacatttcg
    13981 gcgatcctcc ctattcctgc ttctccatgt ttgcttcact ccacagggtt aatgattctc
    14041 gcgacctcta cctcattgat tgaggcatct cgtattcagt tatttataca aaaaaagttt
    14101 acgatactcg agaggctttg tcaatctata actgctgttt tttgaaaaaa tactgataaa
    14161 tatagaaatc tttactttta ccgaaaaaac aatcaaaatg tgaatcatct atccattgta
    14221 aacgatagta taattataca caaaaatgta tgtaatgcaa taaaaaaaga actagaacag
    14281 aaaatttcct gtgtccagtt ccatctcgat catttaagct gccgctttct ttggctcacg
    14341 tcctaggatc gcttgcaaaa gaacaacgac gccataaatg gctaatatta gataaatcaa
    14401 acttgagtat tgggtgattc cccagcccca tgttcgatat acggctaatg taatggctaa
    14461 aacaattgcg gcaatcacgt tgaataaagc aaaagcccaa agatttccat tttctttgcg
    14521 tacaagataa aaaacagaga aataaacgct taacgctaac caggcgatac aagccagaat
    14581 aacgccccaa taaataaaga atggtaacat atcgattcac ctcactttta tacagcagta
    14641 ttgtaacacg ttttcattct tttgtcagtg aacattgtga tgaattgctc aaatgcccat
    14701 aacacttttt tattcccatt tttctgactt ttcaacaata aatttgttcg agtatgggct
    14761 ataatggaaa gtgaggtgac aaaacatgaa acaattaaca gtagacgaag cagcaaaaga
    14821 gatattagct tatcagcaaa ttacattttt gaccggtgca ggggtatcca caccttctgg
    14881 agtgccagac tatcgatctt tgtcaggaat ctaccacggg atggagcagc cggaatactt
    14941 gctgagtcat caagcaatgg tgaatgaacc ggataaattc tatcaatttg tcaaaaagat
    15001 ttatcatcct gaagcaaaac ccaatatcat tcatttggaa atagcacatc tagcgaaaga
    15061 aaaaaatgtt tgggttgttt cccaaaacat cgacggtttg cacgcaaagg ctaaaagccc
    15121 gcaactcgtc aattttcatg ggagtctgta tcaatgtcat tgtcgaaagt gtcaacaaac
    15181 ggtagactgg aaagaatatc ttcacagcga caaacatcaa gcttgcggag gacaaatacg
    15241 cccagacatc gtgttatatg gtgaagggtt tcaagatgga gtcatggagc aggcggctta
    15301 tgccgtcagc caagcagagc tgatcgtcat tgttggaacg agttttcaag ttcatccgtt
    15361 ttgtgatctg gtacagtttc gccagccgtc agccaaaatc ttagtaatca atcaaacgcc
    15421 tgtttattta gcggagcctt atacgtttat tcaagaagac ggagcggttg ttttcaaaaa
    15481 aatacaagaa ttaggagcag attatgactg aattcaaaac cgaaaaagaa aaaatgattg
    15541 caggcgagct ttattttgcc aatgatcctg agctggttgc cgaccgtatg tttgcaagaa
    15601 gtcaaagcca aatcatcaac caagcagaat cagcggaact gcgatcccaa ttgttgaaag
    15661 agacttttgg ccgtacaggg aaaaaaatct atatggagcc agttatcaat tttgattacg
    15721 gatataatat atttgttggg gagaattttt atgcaaattt caattgtaca tttttagatg
    15781 tcagcacgat tgaaatcggt gataactgca tgttcgctcc gaatgtccag ctttacacgg
    15841 cgacgcatcc actacatcca gtgaaacgta acagcggtct ggagtacgca aaacccatta
    15901 aaatcggtaa taatgtttgg ttaggcggag gagttatcgt cacgccgggt gtcactctag
    15961 gggacaacgt agtagtcggc gcaggttcgg ttgtgaccaa atcttttccc gacaatgtag
    16021 tgattgcagg aaatcctgct cgtatcatca agacagtaga ggaagaacta acagaagagt
    16081 ctcttgaaac attacgtcat aagatcgata tgatcgatcg agagttggtt gctttattag
    16141 aaaaaagaat ggatacggta actaaaatag gacaagtgaa aaaagaagaa aaccaggcag
    16201 tttatgatgc aaaaagagag cagcaagtat tggataaagt tgttagtctt ctgaaaaata
    16261 agggatataa ggaaacaata acagacacgt atgttgattt gatgaaacac tcgcgcgaat
    16321 accaaaacaa aatgaaggag gaataagtgt gatcgaagga gagacacggc gaaaacaaat
    16381 catgcaaacc ttggcaaata cagaaaaacc agtgagtgct agcaagtttg cgaaaacttt
    16441 tggagtcagc cgtcagatca ttgtaggcga tattgcttta ctacgagctg caggtaaaag
    16501 tatagtggca acagcaagag gttatatcct cgaaacagaa gaaaccaata ctgggcacat
    16561 cagtaaaatc gctgtccagc atggaaaaga tcaaacagaa gaagaactga gactaatcgt
    16621 tgaaaatggt ggagaaatca ttgatgtaat tgtggaacat cccctttacg gtgagttagc
    16681 aggaactttg cacatcaaaa cgccacaaga tatcgattcc tttatgagac gttatcaaaa
    16741 aagtaatgct actttgttat ccgaattaac aggcggcatc catctgcata cgatccgcta
    16801 cccagaaaag catatgctga aaaacatcaa acaaaaacta gctgaagcag gaatcctcta
    16861 cgaaggataa catcgtagct gctacttttt atcctagaaa ctattctatc atctctgaga
    16921 taaacaccca tatggaaaaa ataagaaaag atgcgtatcc aatacacaga attttttgga
    16981 attgcgattt ctagatgtct atctaaattt cgattagtgg agaaacataa aaaagggaag
    17041 tgcagctttt gagcactccc cttttttata aagctttctt gtagcatagt atcgaaggaa
    17101 cctgccaatt ctctactcga ttaccaatac gccttctttt agtgcaaatg gattttgatc
    17161 gttgatgtgg tcatagaaca tcacaccgtt gatatggtcg atttcatgtt gaacgacgat
    17221 cgattcataa ttcttcaaac gaatcttgtg tttttcacca ttcatatcat aataagatac
    17281 agtgattttg gcatggcgaa caacatatcc tggtacttct cgatcaacag ataaacagcc
    17341 ttcaccttcc cctaaacaag catcttgaac agaatggctc aaaattttag gattatacat
    17401 aacagtactt agagaaggtc catctgcctc tgggtcagga cttggtacgt gaacagcaat
    17461 gattctttta gaaatatcta attgaggagc agctaagcca acaccgcctc gtaagtgtag
    17521 ttcttcagct ttgattggat cttggctgtt ttttaagaac tcaagcatct cttttcctaa
    17581 agagatatct tcttcgctta atggaagaga aacttctttt gcaacttcgc gtaaggtcgg
    17641 atttccttca cgaatgatat catccattgt aatcatggaa tatgtacccc tttcttaaaa
    17701 gactgtgttt caagtctatt ttcgcattat agtttaccac aaaaatggaa aagtagtagt
    17761 aagattgttt gaaaactacc ttttcttaat gattaactac ttgattttca acccggtttc
    17821 aggtaaagta tagctgtgtg ctctaggcac atgacctgcc acttggttaa gcgaatcacc
    17881 aacgttttac tcagtcgcag gggaataatc gaaagaggtg aaagaaatgg caatttcaaa
    17941 agaacgcaag aatgaaatca tcaacgaata cgctcgccat gaaggggata ctggttctcc
    18001 agaagtacaa atcgctgtat taactgaaga aatcaaccac ttgaatgaac atgcacgcgt
    18061 gcataaaaaa gaccatcatt cataccgcgg cctaatgaaa aaagttggtc accgccgtaa
    18121 cttgttagct tacttacgta aaactgacgt tcaacgttac cgcgaattga tcaaacgttt
    18181 aggcttgcgt cgttaattgt agtcaaaaag agtgggattc acatgaatct cacttttttt
    18241 actcgtatat caattaagta aaattccgtg atcaagctag atggactcct actaaccaaa
    18301 tgaaagctgc tcaaaagacg gctttcggca gttttcattt gtttagtagc gtcttgcttt
    18361 cacgagaagg aaaaggagaa gttatgtcag aaaaacaagt attcaaaacg acatggggcg
    18421 gccgtccctt acaaatcgaa gtcggtcagc tagcaaaaca agcaaacgga gccgttttag
    18481 tccgttatgg agatacagta gtcttaagtg cagctgtggc ctctaaagaa gcaaaagata
    18541 cagacttttt cccattaacg atcaattatg aagaaaaaat gtatgcagca gggaaaatcc
    18601 ctggaggttt catcaaacga gaaggccgtc caagtacgga agcaacattg actgcccgtt
    18661 tgatcgaccg tccaattcgc ccgatgtttg cagaaggctt ccgtaatgaa gtccaagtaa
    18721 cgaacatcgt gatgagtgta gaaacagact gctcgccagc tatggcagca atgctaggtt
    18781 cttctttagc actatcaatc tcagatatcc catttgacgg cccaattgca ggcgtagaag
    18841 ttggtcgtgt aaatggcgag tacgtcttga atccaacagt agaacaagca gaacaaacag
    18901 atatcgaatt gactgttgcc ggaacgaaac aagccatcaa tatggttgaa agcggcgcga
    18961 aagaagtttc agaagaagac atgcttggtg cattgctgtt tggttttgac gctatcaaag
    19021 agttagttgc tttccaagaa gaaatcgttc aagcagtagg gaaagaaaaa atggaagtga
    19081 ctttactgca agttgatgaa gtattgaaaa aagaaatctt tgatgcttct tacgctacaa
    19141 tgaaagcagc agtaatgact gaagaaaaac ttgctcgcga agataatatc gagcaagtga
    19201 agatcgatat ccgcgaagct tatgctgaga aatttgcagg tcatgaagat gaagatcact
    19261 tgttgaaaga agttaaacaa atcacagaag atcttgaaaa agatgtcgta cgtgaattga
    19321 tcaccatcga taagatccgt cctgacgggc gtaaattgga tgaaatccgt ccattgtcat
    19381 cagaggtcag cttattgcca cgtgtacatg gatcaggttt gttcacacga ggacaaacac
    19441 aagctttatc agcttgtaca ctcgcacctc taggagagca ccaaatcatt gatggtttag
    19501 gggtggaagt cagcaaacga ttcatccacc actacaattt ccctcaattt tctgttggtt
    19561 ctacaggacg tgcaggctct ccagggcgtc gggaaatcgg acatggcgca ttaggtgaac
    19621 gtgcgttagc acaagtaatc ccttcagaag aagaatttcc ttatacgatc cgtttagtag
    19681 cagaagtatt ggaatcaaat ggttcttctt cccaagcaag tatctgtgct ggcacattag
    19741 cattgatgga tgcaggtgta ccaatcaaag caccagtcgc cggtattgcg atgggacttg
    19801 tttctgatgg tgaaaactat acgatcctaa ctgatatcca agggctggaa gatcatcttg
    19861 gcgatatgga ctttaaagtt gccggtacaa aagatggtat cactgcttta cagatggata
    19921 tcaagattca aggaatcaca gaacaaatcc taactgaagc attaactcaa gcaaaacaag
    19981 cacgtatgga aatcttagaa gaattgactt caacgattgc tgctccacgt gaagaattaa
    20041 gccaatatgc accaaaaatc gaaatgatcc agatcgaacc tgctaaaatc aaagatgtta
    20101 tcggtaaagg cggcgatacg atcaacggaa tcatcgatga aactggtgtg aaaatcgata
    20161 tcgatcaaga tggaaaagta agtattgctt cagctgatgc agaaatgatc aaaaaagcta
    20221 tcaagatcat cgaagatttg accaaagaag tcaaagttgg tgaagtttat ctagggaaag
    20281 ttgtccgtat cgaaaaattc ggtgcattcg tcaacttgtt caaaggaaaa gatggacttg
    20341 tccatatttc ccaattagca aacgatcgtg tcaataaagt cgaagatgtt gttaaactag
    20401 gtgatgagat ccttgtgaaa gtaactgaaa tcgataaaca aggacgcgta aatctgtcaa
    20461 gaaaagcaat gttgaacgaa gatggttcaa cgaaaaatga agaaaaataa tttaattcat
    20521 aggaataatg tacttgtgat cgtgaaggtg acagggggac aataattatt cgaaaaaagg
    20581 ggactgtgac aaaagccgtg tcacagtcct tttttttcga ataaacggcg ttcaaaagct
    20641 tgcatcaagt aataagtcca gccaagttaa aaacattagg agctgttttt acttggctgt
    20701 tcttattact tgatgcagaa cagctttttt acaacctctc caataaacga tgttcaatca
    20761 gctgtccctc gatattagct caaaaaaaac ggcaaaatat gaggaactat ttttccgttt
    20821 gtttctctaa tactcaggtc aaaccgctga tttcacaacc tctgattcac ggtgttccag
    20881 aagcagcttc tacggaaata agctgaacac ttttgttaca acttcttttt ttctggagtt
    20941 tcttggcgca tatttttgga gtccttctga ataaagcgta aaataaaagt gagaaatatg
    21001 gataaaggag ggaaaggaga tgctgaattt tttatggtcg ttatttatcg gcggtatatt
    21061 aggtttgata gctggcgcaa ttttaggaaa ggaagtcccg ggaggcgtat ctggaaatat
    21121 cattatcggt tttttaggta gttggctcgg cgaattttta ctaggaccac tgggaccgga
    21181 actcggcggt ttttatttga taccagcatt attgggtgca attttttgcc ttgctcttta
    21241 ttcatttgtg gcaagacgta tcagagaacg tccaaagaaa gaataatttt attcaaataa
    21301 gttgacactt cagtgtgtta cttggataat tagaagcaat ttaggaaagc gggtgtcatg
    21361 gataagaata tcatctatca ttcaagctta cctaaaggat atccatataa caagaaatga
    21421 gggaaaaacg tggcaacaac gcacaatacc aaaaagatat caatggctgg cctgcttgta
    21481 gcgatgggag tcgtctacgg agacatcggg acaagtccgc tttacgttat gaaatcgatt
    21541 gttgaagata atggcggatt gtcaggaatc aatcctgatt ttgttatcgg atcagtctca
    21601 ttgatttttt ggacattgac tctactgaca acaatcaaat atgtcgtgat tgcattgaat
    21661 gcagataacc atggtgaagg tggaattttc tcactctaca cacttgtccg aaaaggtggg
    21721 aaatatttga tcattcctgc aatgatcgga ggagctgcct tactagcaga tggggtattg
    21781 acccctgctg tgacggtaac gactgcgatt gaaggcttgc gaggaattcc gcagtttttt
    21841 gaccggttcg gtaataacca gaacgtgatc gtcattatca ctttagttat tatttttgtt
    21901 ttattcatgg tccaacgctt tggtacagat attgtcggaa aagcattcgg accaatcatg
    21961 ttcgcttggt ttactttttt aggcgtaatg ggattaatga acttcagtca agactggaca
    22021 gttatccgtg cactgaatcc ttattatgct atccagctgc tttttagtgc agataacaaa
    22081 ttaggcttgt tcattcttgg aaatattttc ctggctacga caggtgcaga ggcgctttat
    22141 tccgatcttg gacatgtcgg caaacgaaat attcgtctga gctggcctta cgtcaaaatt
    22201 tgtttggtta tcaattattt tgggcaggct gcgtggttgt tgaatgttta tcaaaaccct
    22261 gaagcacagc aaatcaagaa cctgaatcca ttcttccaaa tgatgcctca aagttggatc
    22321 gtgatcggcg ttgcttttgc aacagtcgct gcagtcattg cttcgcaagc attgattaca
    22381 ggttcgttta cgctagtttc agaagcaatc aagttgaaac tgctgcctcg gttaaagatc
    22441 atgtatcctg ggaacagtat cgggcaaatg tatatcccag cagtcaatct gattttatgg
    22501 atcgcctgtt ctctagtggt tgtcacattc cgaacgtctc atcatatgga agctgcttac
    22561 ggtctatcga tcactgtgac gatgttgatg acaacagcac tgctttactt ttatcttatc
    22621 caaaatgggc attctcgttg gcttgcatat ttagttacct ttttcttcgg tgcgatagaa
    22681 gtcgttttct ttatctcaag tgttgtcaaa ttcttccgag gcggttttgt agccgttctg
    22741 attggtttga tcatcttagt cgtgatgttt atttgggaac aagggaacat tattcgcgaa
    22801 tcgtcaaccg aggatgtgtc tttgagagat tatatcccgc agcttagaga attacgtgat
    22861 gatacttctt taccaatcta tcagacaaat gtggtcttct tggttcctga tatggctgaa
    22921 gataaagttg gccgacagtt catctattcg attttagata aacggcctaa aagagcacga
    22981 gtctattggt ttgttcatgt ggaagtgacg gatgagccat atacgaaaga atatcaagta
    23041 gatatgatgg acactgattt tattgtccaa gtgaatctgt tcttaggttt cagggtccaa
    23101 caggaaatca acgtatatgt tcgccaagtc atccatgatt tgatgaaaca aggccgtttg
    23161 ccaaaacagc cacaaagata ctctctgaca ccagggcgag aagttggcga tttccaattt
    23221 gtcctgattg atgaagtagt ttccaatgtg acaactctag gaaaatggca acgtcaaatc
    23281 atgcaggcaa aactggcaat caaaaaaatc gctacctcgc cagaaacatg gtttgggcta
    23341 gagtacagcg aagtaaaaca tgaaactgtt ccgcttatca ttggaaatac tcggaaaaca
    23401 tggttgaaag agcggttgtg aaagtagcgc tttgacctga attaatgtcg aatgtcagct
    23461 acttgaacac cgtttatcag aggtcatgaa aaagctgtac tgcaacaagc agtaagaacg
    23521 agcagataaa aacaacttct catgtttttc atctgtttgg gcttaatgcc gcagatgcaa
    23581 gcttttgaat accgttttta tcagaggtca tgaaaaagct gtttagcaac aagtaacgca
    23641 atgattttga agtgataagg agatgtggct gaaaaatgcc gcatctcctt ttttattcct
    23701 aaatatgaat acgcattatt ttcaatcttt ttgtagaaaa agggtacaat agaaaaagag
    23761 aaaggcggga gaatatggtt atcaatttca ttattttatt tttgatgatt gcaattactt
    23821 cattttttgt agcaagtgaa tttgctttag tgaaaatcag acgatcaaga ttggaacaac
    23881 tagaaaagga aaatgtaaag aacgccaagc tagctcttca tgttacacac catttagata
    23941 actatttatc agctagtcaa ttaggaatta cgctaaccgg tttgatcatc gggtgggtcg
    24001 gtgaaggatc tgtcgcggct ttactagaac cagtgatcgg taaactacct tttagttcgg
    24061 ctattagcag tacaatctct gtggcgctgg gatttatttt agttacgtat attgatgtgg
    24121 ttattggcga actgcttcct aagagctata gtatcgtcaa tacggagaag gtcgtcttgt
    24181 tcgtagttaa accacttcat tatttttata aagtaatgtt tccttttatt tgggtattga
    24241 atcattcagc tgcagggcta gggaagctat taggtgtgcg tctcgtatct gaaggagaag
    24301 agacattgtc tcaagaagag ctgacattag tcgcattgaa ttcttatgaa aaaggagagt
    24361 tgaccaaaga agaatatcat tatttggaaa acgtgtttga atttgatgat accttggcaa
    24421 aagatataca agtagaccgt acgtccatgc aagtattcga agcttcagaa accgtcaaag
    24481 aagcgataaa agcttcgatt gaacaaggac atactcgcta tccggttatc ttagaatcaa
    24541 aagacaatgt tttaggttat gtcaccttgc cagatttgat caagcaatct tataaagatg
    24601 accagctgac agtagaacag ttgattgaag agccgatcgt aacaactgaa acgatcccta
    24661 taaaaaaatt attgacgatc atgcgaaaaa agggaaagca tattgctatc ttaaaagatg
    24721 aatacggagg gacaagcggc ttagtgacga ttgaagatat tttagaagaa atcgtcggcg
    24781 aaatccgaga tgagacagat ttggacgaag cattgatagc ggaacagtca gatggctctt
    24841 atatcatctc cggcaaacta acattagatg atttccagcg ttattttcat gtagagattc
    24901 cagagtttga agaaacgaat tttactacac ttgcgggctt tgcttctagc cgatataaag
    24961 aaataaaagc aggaacaatc attgagattg catcattccg tttcacggta ttagaatacc
    25021 agcatgcaca tatcgattat ttcaaagtag agtccacgga aagaaaaaca gaataaaaga
    25081 aaaaaggctg tgacattttt tgtcacagcc tttttgttat ctaaaaaacc tgttttcggt
    25141 aaacgaagaa gatattttca ataaaagttt tcatttttag ccgcaacgat ttcttttaat
    25201 caaggctatc ttgcttctgc atgttctaat gtgaaatgtt cctcttcacc catgtgtttc
    25261 aattgtttca tcgaacgcat ataagagtaa agcaacacgg caacagaacc gatccaagct
    25321 aaaggagaag ctgctgctgc tcctgggtat ccccacattc ctgtaagaac gatagctgca
    25381 aaagaacgca taagcaattc catcataccg gcgatcgtag ggatcttgct ttgtccaagc
    25441 ccttgaagtg tgtaacgtaa aatgaacagg actgctaaga tccagtaaag agacccattg
    25501 atattgaagt aggtttgtgc taattcaaat acacgtgttt ctgatgggct aacaaacaat
    25561 tgaacaaaga aatgaccgaa gaaaatgacg atagcacctg ctaagagact aaatccgaca
    25621 ctcatcaata aagtttgacg taccccttgc aagattcggc catattcttt cgcaccatag
    25681 ttttgtgcag tgaaagttgc cattgcaata ccaaatgaca tcatcggctg atttgcaaat
    25741 tggtcgatac gaccagcagc ggcttgagca gctacgacat ctgtccctag actatttagt
    25801 gctgcttgta acacgatagc accaacagcg atgatcgaag attgaaacgc cattggcaaa
    25861 gcagcgttta ggtgtacagc aaactcttct ttttcaaatt tgaaatcttt tttgcgtaac
    25921 tgtaacaaag gcattttttt cttgatatac actagacaca gtacgctgga agaaacttgt
    25981 gcgattactg tagcaatagc tgcaccttcc acacccatgc caaaatagat aataaagatc
    26041 aaatcaagta cgacgttgat gatgaccgca atgatcagaa agaatagtgg tgtgcggctg
    26101 tcaccaagtg cgcgaatgac attagaaagc aaattgaaac tgactgatgc aaagatccct
    26161 aataaaataa ttgaaataaa tgtttgtgct tgatcaagta tttcaggagg agtttgcatc
    26221 aattgaagca tcggacgaat aaacaggaga cttaggacag ttaaaatcac agtgacgaca
    26281 agactaatga caacactagc agcaaaactt ttcttcagac cccgataatc ttttgcaccg
    26341 taacgttgtg cggtaataat ggctaaacca gctgttaaac cttgggcaaa gccaatgatc
    26401 aaaaatgtca ggcttccagt tgcaccaacc gctgccagcg catttttacc tagcgtctga
    26461 cccacgatga tcatatcgat catattgtag aactgctgaa aaacatttcc gatcagtaaa
    26521 ggaacagtga acatcaaaat cagtttagcg gggcttcctt ttgttaaatc gcgcatcttt
    26581 taaactcctt atatctttaa aatttagtta gtagtatata gcgtttatcc tgagaatacc
    26641 aattagaaat caatgataat ttatgaaact ccataaaaag aagccaagaa ttgagtcaaa
    26701 gaatcaacaa cttggcctgg tctgtaaaat aagggacatc cacgtttatc tatcacctta
    26761 atattgtctg atctcacttg gtaaaaggat cgtttctctc gtaccgtcat cataggcgta
    26821 tacttgagca ggaaattggt tcgaataggc aaacggcaaa tcaatatcca tttccacatt
    26881 gcttcctgct tgagaaaaat gcatcgtcac ttttccacga ttttcgatca aatcaaaggt
    26941 taatatattt tctaatggaa tgactccctt aagatctagg tcaataataa accatatact
    27001 gtcaatcaat tcatctggca aacttgatac tacaccaact gaagcgtaac ggttgcggct
    27061 gctatcaaaa ctttcaaaca tctgtttaac ctccttcgaa tcaacgaatg ttttattttt
    27121 ataattaatc ctaggataaa ctagaaatac ttacttcaac gagggttcat tctaataaag
    27181 aatcaggaaa aatgcaacaa taattgaata ctttttttca aagtataaat ttttataaag
    27241 gtacgataaa agtatgcgag gaaaagcttg gtaaattagg atttttcgtt tgcaaagcta
    27301 tttgtaaaag tggaagcctc atatgaaaat aaacgattag aaagaaaaaa aggcgtgtta
    27361 aaagctgctg acccgccgct tctaacacca cctgattgtt tcagtattat tcttctgttg
    27421 aaccgattac ttctggttcg ccaccttcac ctgtgctttc ttcttcttca ctttgagcga
    27481 ctacagcagc gatttgctct tcactgtcgg tcacgaattt gtaatcgtcg ttttttggaa
    27541 gatcggctac tgtaatagaa tcacctaatt ccaagtttgt cacgtcaact tcaattcgtt
    27601 caggcaattt gtctggtgta gcagatacga ctactgtata caagttttga gctaacactc
    27661 caccagattt agcacccact gattcgccaa caagaacgac ttctgtttcg acttctgttt
    27721 cttccgacat gtttacagat agaaattctg catgttcgaa atgattggta aaagtgttcg
    27781 tttggatctt gtgaaccaat gtattgactt tttgtccatc gatgtccatc gtgatgactg
    27841 tgttcgctcc atgctcacgc aacaatttgc ttagtgtcaa accatcgaat gagatagggg
    27901 tactttctac tttataccca taaacaacag ctggaacttt cccttcgtga cgaagtttgt
    27961 ttcttaatga acggggacgt acttctcttt tacttacttc taatgatact gacataacca
    28021 aaattcctcc ttaaaatgtg actgtctgct ttggtaacgg acagatttta tttaagtgaa
    28081 ttctaaaggc taatctcgtg cacatcatcg aaggcagggc gcttaaaaaa gcgcaaaagg
    28141 agagatgcgc gcatctcccc ttagaaagtc aggattcaac ttttctccac caggtcatta
    28201 cgctgtgtat gagaaatcca attcacttta ctatttaacc gtaacgtgaa ctgttaatta
    28261 agtcaaggaa taactatgat gatgtatttg caagcaagaa tattcaaggt ataattaatt
    28321 agtagtaata agaaaggaaa cagcatgcgt ttagataaat taatagaaga aaaattagaa
    28381 acgacacgca aggaaatgaa gcggctgttt gctttgaagc aagtgatgat agatggcaca
    28441 gtcgaatttc gacaaaatcg aaatgtagat agtttactgc atcaaatcga agtagcaggt
    28501 gaacacttag aaacaaatga gtcctattat cttctcaata aacctgaaaa agctgttaca
    28561 gctgtatcag atccagaaaa gacgacagta atcgatctga ttgcaagtgc cgatcgtttc
    28621 caaccattat atccagtggg aagactagat agagatacaa caggactatt gctgatcact
    28681 tcgaatggac aattgggata tgaaatgcta cttccagaaa aaaaggtttc aaagacttat
    28741 caagtagttg tgaacgaaag ggtgacaccg gcagacgtaa cagcatttga aaatggaatc
    28801 gtattcgctg atgggtatca atgtaagccg gctgatttaa aaatactcaa agcagacaag
    28861 caccaaagta cggttttatt gacgatccaa gaaggaaaat tccatcaagt caaaaagatg
    28921 ttcttagccc aaggaaagaa agtgaccgca ttgaaacggc taagcatggg accattggaa
    28981 ttagatgaac agctaggagt aggcgagtat cgatcgttga cattagcaga gatgaaaaag
    29041 ttaagtattt attttaaata ggaggtcaaa aagatgaaat ataaaacttt attgttcgat
    29101 gtagatgata cattgttaga ttttcaatta actgaagcca atgctttaag tgctttgttt
    29161 gcagaacaag gaatcacatt gacaccagaa atcgaaacca gctataaggc tttgaatcat
    29221 catttatggc aggagtttga aaaaggcaga ctatcacgag aagaaataat aggcagtcgt
    29281 tttgggttac ttttccagca atttgggaaa acagtagata gtgatcaaat ggaaaaacgc
    29341 taccgccact acttgaacca aggtcatgat cttgttccag gaagtctaga aatcctgaaa
    29401 aaggtccatc cgcatgctga actttatatt gtgacgaacg gtgtgtcgca cacgcaacat
    29461 cggcgattaa cagatgcagg aatgcttgat tacttcaagg atatctttgt ctcagaagct
    29521 gtcggagcgc aaaagccgat gaaagaattt tttgatcatg tcttttccca cattcctaat
    29581 tttgataaaa agcaaactgt gatcattggt gattctttga cttctgatat caaaggagga
    29641 aatatggcag ggatcgatac gatttggttc aatagaaata aattaccaga aatcgaagaa
    29701 atccagccta cttatcgaat cgattcatta gaggaacttt attcattatt ggaaattgaa
    29761 aaggattaat tttcagaaaa atatagcatt ttcgtgtttt ttcgctataa tagattagaa
    29821 tgaaaaaacg gatacgaaag aggttttagt caatgacaaa aagcaagcca attatcattg
    29881 gtgtgacggg tggatccgga agcggaaaga caagtgtcag ccgtgcgatt ttcaacaact
    29941 tcccggacca ttccatcatg atgcttgaac aagattctta ttacaaggat cagagccatt
    30001 tgagttttga agaacgattg aacacaaatt acgatcaccc atttgccttt gataatgatc
    30061 tgctgattca gcatgtaggc gacttactga actataaagc cattgaaaaa cccgtgtatg
    30121 actatgtagc gcatacaaga agtcaggcaa cgatcattca ggaaccaaaa gaagtgatca
    30181 ttctagaagg aatcctgatt ttagaagacg aacgtctgcg tgacttgatg gatatcaaag
    30241 tatacgtaga tacagatgat gacattcgta tcattcgtcg tatcaaacgg gatatggaag
    30301 aacggggacg cacattggac tccgtgatcg aacaatattt gacagttgtt aaaccgatgt
    30361 atcatcaatt tatcgaacca acgaagcgct atgcggatat cattgttcca gaaggcggag
    30421 aaaaccacgt tgctattgat ttgattacaa caaaagtagc tagtttctta aaccataaat
    30481 agtgcgcagc gaaacagttg aacggactgc gaccgtttga aaagcaagga ttctgctttt
    30541 tgaacagtca ccagccaact gtttttttat ttataacaag aggttgtgaa aacagcatct
    30601 tgacctgagt attaaaagaa gaaacggaaa atagctgttt atattttacc gttttttgag
    30661 ctaataccga aggtcagctg attgagcacc gtgaagtaga ggttgtaaaa aagctgtctt
    30721 tgcattaagc gataagagca tccaaataaa acagctcctc acgtttttcg ttgggttgga
    30781 cttattatcg tagatgcaag cttttgaaaa ccgtttaacg agaggttgta acagaagtgg
    30841 tcagatccaa taaataagaa gaaacttacg aaaattattt ctcgaatttg tgtgagattt
    30901 cgacttattt ttttgaagaa gctcttttgt cgcaccccc
//
LOCUS       DACEPD010000025        30721 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.025, whole genome shotgun sequence.
ACCESSION   DACEPD010000025 DACEPD010000000
VERSION     DACEPD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.025"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(256..810)
                     /locus_tag="IW797_001106"
     CDS             complement(256..810)
                     /locus_tag="IW797_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ1223893.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(936..2864)
                     /locus_tag="IW797_001107"
     CDS             complement(936..2864)
                     /locus_tag="IW797_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ1223894.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(3170..3886)
                     /locus_tag="IW797_001108"
     CDS             complement(3170..3886)
                     /locus_tag="IW797_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1223895.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3887..5710)
                     /gene="glpO"
                     /locus_tag="IW797_001109"
     CDS             complement(3887..5710)
                     /gene="glpO"
                     /locus_tag="IW797_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ1223896.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5724..7220)
                     /gene="glpK"
                     /locus_tag="IW797_001110"
     CDS             complement(5724..7220)
                     /gene="glpK"
                     /locus_tag="IW797_001110"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ1223897.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7356..8816)
                     /locus_tag="IW797_001111"
     CDS             complement(7356..8816)
                     /locus_tag="IW797_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1223898.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSSFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8806..10101)
                     /locus_tag="IW797_001112"
     CDS             complement(8806..10101)
                     /locus_tag="IW797_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1223899.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10433..11110)
                     /locus_tag="IW797_001113"
     CDS             complement(10433..11110)
                     /locus_tag="IW797_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ1223900.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11140..12231)
                     /locus_tag="IW797_001114"
     CDS             complement(11140..12231)
                     /locus_tag="IW797_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ1223901.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12332..12766)
                     /locus_tag="IW797_001115"
     CDS             complement(12332..12766)
                     /locus_tag="IW797_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1223902.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13350..14807)
                     /gene="ascB"
                     /locus_tag="IW797_001116"
     CDS             complement(13350..14807)
                     /gene="ascB"
                     /locus_tag="IW797_001116"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1223903.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14804..16699)
                     /locus_tag="IW797_001117"
     CDS             complement(14804..16699)
                     /locus_tag="IW797_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1223904.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16757..18532)
                     /locus_tag="IW797_001118"
     CDS             complement(16757..18532)
                     /locus_tag="IW797_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1223905.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18582..20078)
                     /locus_tag="IW797_001119"
     CDS             complement(18582..20078)
                     /locus_tag="IW797_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1223906.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20272..21894)
                     /locus_tag="IW797_001120"
     CDS             complement(20272..21894)
                     /locus_tag="IW797_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ1223907.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(22222..23535)
                     /locus_tag="IW797_001121"
     CDS             complement(22222..23535)
                     /locus_tag="IW797_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1223908.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23780..24004)
                     /locus_tag="IW797_001122"
     CDS             complement(23780..24004)
                     /locus_tag="IW797_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223909.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(24024..24392)
                     /locus_tag="IW797_001123"
     CDS             complement(24024..24392)
                     /locus_tag="IW797_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1223910.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24588..25298)
                     /locus_tag="IW797_001124"
     CDS             complement(24588..25298)
                     /locus_tag="IW797_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223911.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25316..25813)
                     /locus_tag="IW797_001125"
     CDS             complement(25316..25813)
                     /locus_tag="IW797_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1223912.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25850..26302)
                     /locus_tag="IW797_001126"
     CDS             complement(25850..26302)
                     /locus_tag="IW797_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1223913.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26437..27042)
                     /locus_tag="IW797_001127"
     CDS             complement(26437..27042)
                     /locus_tag="IW797_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1223914.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27492..28112)
                     /locus_tag="IW797_001128"
     CDS             complement(27492..28112)
                     /locus_tag="IW797_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1223915.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(28114..28983)
                     /locus_tag="IW797_001129"
     CDS             complement(28114..28983)
                     /locus_tag="IW797_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ1223916.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(29006..29413)
                     /locus_tag="IW797_001130"
     CDS             complement(29006..29413)
                     /locus_tag="IW797_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1223917.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29432..30721)
                     /locus_tag="IW797_001131"
     CDS             complement(29432..30721)
                     /locus_tag="IW797_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1223918.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         8712 a         6675 c         5069 g        10265 t
ORIGIN      
        1 tttgacctaa gtattagaga aaaaaacggg aaaatagctc ctcatatttt gccgtttttt
       61 gagctaatac cgaaggtcag ctgattgaac accgtttacc aagaggttgt gagcctacca
      121 tttaactccg aatcacaaga aataatttca aaaacaactt ctcctgtttt tggtttggtt
      181 ggcttattat cgagaagtag ctactgggag tctatttatc aaaaaaagga atgcgacata
      241 ggtcacaccc cttttttatt ttctttgaaa catctttcgg tactcgacag catacaagac
      301 aaaagattgt aaaagaccgt aagtgatgac ggcaaaaatg actctcaccc acagcgttgg
      361 taattctggg aaaagcagca aaaggatcat gccaacatag atgacagcag tcgctacttt
      421 gccataccac ttcgctccac ttaatttgat atttttagat agcccgatta atccagctac
      481 aaacataaag ccttctttta tcaagaatag accaaacaac agccaaagcc acggtcgata
      541 ccatgccata gacaaaatca gaaaaagttg tgtcaattta tctgcaaacg gatcaagcac
      601 cttccctaag tctgttacct gattatatct ccgagcaatg accccgtcta gataatcaga
      661 caaacctgag acaacaatga ccgctcctgc tatataaaat gactgtctca gcacaaaata
      721 aagataaatc ggcagcaaaa aaatacggaa tatcgataaa agattcggaa tcgtttgtat
      781 ttctttacgc cagtctgtgc ttaatttcat tttatcgccc tacctcctca gcactctttt
      841 ctttttattt tagcttacac ggggaataaa accaatgatt tcactgtttt tatttttctt
      901 tacagccact gatattatga gctaatttga gataatcact tgctgttttc atcgtttact
      961 cttctttctt cctgtcgttt gcgcatttct tttactagta ctttatgagc tttttttgat
     1021 cctcgctcta ggtcacgctg ccgtttacgt gttttataat cgataaacag actgtctaaa
     1081 tcgtaacctg tcgggtacag atcttctgct gaacgcacca gacgaacacg ctgacgaggc
     1141 acgtggatta tttttttgtc taaatataca tcaatcgtat cttcacctat atcttcataa
     1201 actaacgctt ctttttggta ttcattgaca aatatccgat caccttttgc aaagagctgg
     1261 cgttcttttt tattctcctt cctatttgta actgctgtct taggaagaaa ttcttttttc
     1321 tgtgtccgat aggctttttc cgccaagtaa cgctctgctt gatggatcaa tttcatcgac
     1381 atatccattt tatgagcaat ccacaaagct tggctttctc ctgtttctcc aacccgcaac
     1441 tgatatttag gacgaagtgc ttcccgatcg aaagccatag cagccggcac aaaatcttca
     1501 tgatctccgg cgaatttctt gatttctcca taatgggtcg tggcaatgat caatgcccct
     1561 tgctcataca tagattccat gattgcgatc gctagtgcag ccccttcatt tggctctgtt
     1621 ccactgccaa tctcatctaa aagtaccaac gtattccttt ttatcttttt caaaatagca
     1681 gcgatgtttt gcatatgacc agaaaatgta ctcaacgcat tttctaaatt ttggtggtca
     1741 ccgacatcga cgaaaatctc atcaaagaca gcaagctctg tcccttcttt tgccggaact
     1801 tgcataccga acatcgccat caaagtcaat aaaccgactg tcttcaacac gactgttttg
     1861 cctccggcat ttgctccggt gataatcaag cctcgatagt ctttaccgat ttcaacatcc
     1921 aatggtaccg catgctcagg taaaaatgga tgtctgcctt gcttgataat gatatgttcc
     1981 gatttattga ttctaggtgt gcttccattg atttctcggc taaattttgc ccgagcaaag
     2041 atgatatcta acaccgtgat cgtttcgatc aataaatcga tcacttcttc gttttctgcg
     2101 atagcaccag tcaatgccgc caggacttga tattcttcac tgatttcttc agcttttaac
     2161 aactgatagt gttcattcaa tttagaaatc acagttggtt caataaaaac cgtcgttctt
     2221 ttatttgatt catcgataat cgttccatct attttatttt tatagctggc tttgatagga
     2281 atcgtatagt attcgccttt ttgaacaatc attgcttctt ggatcatttc tttgtttttc
     2341 ggatgacgca agaatttcag taattttgac tggatttctt tttcgattag ttgtaattgt
     2401 ttgcgtactt tacgcaaatt acgtgacgcg tcgtcactga ctttattatt ttttattttc
     2461 tggtcaatca actcttctac atttatcaaa tcaggaagat gtttgctata agcaaacaac
     2521 aagggtgtct ggtattgatt cttatcaaaa aactttgtga tcatccgact gcttctaaga
     2581 aaatccgcat attcgatcaa atcggttggc tgcagaatca accctttttt gacttgttct
     2641 gtcagtgcat cgatcctcgg caaccccata aatggaacat gctgactgct ttctaagatc
     2701 aagcgtgctt ctcttgtttc ttcttgacga tccaagacag tttgtaggtt tgtcgagacc
     2761 gtcatatcgc tgattcgttt tttgctatag tttccaattg ctcttgcttg gatttccttt
     2821 ttgatccgat caaattgtaa ttgactaatc atttcgttgt ccatattctc tccttttctt
     2881 gaagagatac ctgtcccaaa ataaataagg gcctagagat aaataaaagg atagaacaca
     2941 gcttaaaaaa gtcatgtcct atccttatta gaatacaaag atattgtttt cctgacaggt
     3001 accgtttcat aagcacacca aataaacttt gtttattggc tattcgactt actgattaga
     3061 taccagctac actcacaaaa cataccccat tcttctaggt cttatttatt ttccacttaa
     3121 ctatagcaag acacattttg gttgtcaaac tttagtatgt tttatagatt tacaccatta
     3181 atgaatagac acctaccgcg ataattgctc cgatcattgg acctgcgatt ggtacccatg
     3241 aatatgccca atctgaagtt cctttattag ctattggcaa taccgcatgt gctaatcgag
     3301 gaccaaagtc ccgagcaggg ttgattgcat atcctgttgg ccctcctaat gaaagaccga
     3361 tggctaagat caatccacct acagcgaata cgttcgttcc aggtgcaaat tcgttttgtc
     3421 caaaagctag caacccaaga actaatacaa atgtaccaat taattctgtg atcacatttg
     3481 ctggataatt ccgtatagca ggacctgttg caaacgttcc taaaatcgcg ctttcatctt
     3541 tcgttgcatt ccaatgtggc aaataagaaa gccaaacaag aatggcccct gcaaaagctc
     3601 ctaaaacttg cgcaataatg aaaggaacga caagattcca actaattgcc cctgtcatcg
     3661 ccatagctag agagactgca ggattcaaat gagctgggcc catgaatcca gaaatataga
     3721 cagccattgt tacagcgagt ccccaaccaa atgcgatgac aatccagcca gaagcctgtg
     3781 ctttactttt gtttaaattc acagccgcac acacaccatc tccaagcaaa actaggatca
     3841 gtgtcccaat aaactccccc atgatttgag tcatatcact gttcatttat ttctccccct
     3901 ttagttcccg cagatctgat tctgcaatca atgcttctaa acgtttagtc tgttgcgcct
     3961 tctcctcttc tgtccattca aaatatccag caattgcatc gatcacaggt tgcttgatct
     4021 catcaagctg atctcgttca aacaacagat gatttgtacg acgaatgaga taatcgcctg
     4081 gggccaaaac catttcttct tctaaaccat aacgtaaccg agctgactca gcgagactca
     4141 aaccaggata aggtgccatt tcttttgcta attcaaagat acgtcgggca ttagtcccgt
     4201 aaaaatcagc gatataagta gcatcttctt cctctaaacc ggctgctact ccttcttttg
     4261 ctaattctgt cactgtttct tctaatttag ttggatcgaa gtttccacct gaaatctgat
     4321 attttttaga gtcgatttct ttcgtctcta ttccgtattc ttcttttaat aattggcgta
     4381 tcaatcgcaa agctccttct gccatcttac ggtaatcggt gattttccca ccagataaag
     4441 tcagcaaacc atctggttct ctttctaaag aactacctct ggaaatcgta gaaggtgctt
     4501 tttcatcacg gctgttttcc aaatggttca acacgtcttc tacttcagca cgagaaactt
     4561 tgttttcctt atattcactt actgtatcga caactttatt gaagctcttg tctgaaatcg
     4621 atccattatc tccaccattg taatcagaac cagaattacc aatcaacagt ggacgaagtc
     4681 ctgcccagct tgcttcgata tcattcaatg tgatatttgc ttctggatag cgatgattga
     4741 tcacatccaa tagataatct acatcttctt gtgtgacttt cggatccgta aaatctcctt
     4801 ggtaatccgt atctgtcgta ccaaagtaag tcttgttttc tcttggaata gcaaaaacca
     4861 tccgcccatc ttgctttcct gtatcgaagt atgtgggttg cggtacaggc agttttttcg
     4921 catctacaac taaatggatc cctttagttg gacgcatttt aggagagact ggacgcgtaa
     4981 aattcaagtt tcgtacttta tctacccaag gaccactcgt attgatcact aattttgctt
     5041 tgatttcgat cacttcatct gtcagcaggt cacgagcttt gacgccaacg atttgatcgc
     5101 catcatataa aaagccaacc gctttcattt tacttactag ataagcccca tcttcggcag
     5161 cctttttgat attatcaatc actaaacggg catcattgtt gcggaaatct agatagacac
     5221 ccgcgccttt tagcccttct tttttcaaaa acggttctct ttccaatact tcttctggtg
     5281 tcagcgtata attctcatat ttggttcctg tcacatttgc cagtttgtca taaaggtcca
     5341 ttgctacttt gaccgagaac atattgaagg ttgttgctcc ttcgccctca tagattggca
     5401 aaagcattgg atctggtttt gggatgtgtg gggcaatact ttgtacgact gcacgttcac
     5461 caactgtgtc agctactact tccacatcaa atgtcttcag ataacgaata ccgccatgaa
     5521 caagtttggt cgagcgagag gaagtccctt ctgcaaaatc ctgcatttcg atcaatcctg
     5581 ttttgattcc tgatgctgct gactggactg ccacaccggc accagtgatt ccgccaccga
     5641 tgatcaacag atccaattct tcttgctgca ttttttgaat gctatcttgt cttgtcttgt
     5701 ttgaaaacat atggatttcc tccttattca aatgcttggg tagcttttac tgcttttttc
     5761 caaccgttgt aaagtttttc tcggcgctct tcctccattt gaacttcaaa tagcttgcca
     5821 gcttcataaa attcgcggat ctcgtctgta tctttccaat aaccgacagc taatcctgct
     5881 aaaaatgctg cccctagcgc cgttgtttcc aaattttctg ctcgttggat cggtacattc
     5941 aagatatctg tttggaattg catcagatat tcattgtttg ctgcaccgcc atctactttc
     6001 aatacaggaa tcttgattcc agtgtcatct tgcatcgtat cgatgatatc tctcacttga
     6061 taagcaatcg actgtaaagt agctttaatg atatcttctt ttgtcgtacc acgtgtcaag
     6121 ccaaacatag cccctcgtgc tgcctgatcc caataaggag cacctagccc aacgaatgca
     6181 ggaacaacat aaacctcatc ttcgcttgta gagcgtttcg ctgcgtcttc tgaatcgcta
     6241 gctttttgca acatttgaag tccatctctc agccattgaa tcgaagaacc agccacaaaa
     6301 atacttcctt ctaaagcata gtaaaccttt ccatttatgc cgtaaccaat tgtcgtcaat
     6361 aaattatttt tggataattg cggtttttca ccggtattca tgacaataaa tgaacctgta
     6421 ccataagtat tcttgaccat gcctggttcg aatgccattt gtccgaataa tgcggcttgc
     6481 tggtctcctg ccatccctga aattggtgct tcgcttccat aaaaatgata accttgcgta
     6541 taaccataaa cttctgagtt agaagtgact tttggtaaca tcactcgagg aatatttagt
     6601 aaatctaaaa tctcctgatc ccaatctagc tgatggatat tgaacaacat cgtccgactg
     6661 gcattcgagt aatcagtaac atgtgcttgt ccatcggtca gtttccatac taaccaagaa
     6721 tcgatcgttc caaacatcaa ttcgcctttt tcagctcgtt cttgcgcgcc ttctacatga
     6781 tcaagaatcc agcgtatctt cgttgctgag aaataggcat cgatcactaa ccctgttttt
     6841 tcgtggaaaa attcttgata gccttcattt tttaagttat ctgcaattcc agctgactgt
     6901 ctggattgcc aaacaactgc atgatagatt ggacgtcctg tatctttttc ccatattact
     6961 gttgtttcac gctggttcgt tatgccaatc ccagcaatct gatttggttt gatccctgat
     7021 tcgatgaacg ctccggcaat aactgactgt acagaattcc agatttcatt tgcatcatgt
     7081 tccacccaac tttcttgcgg gaaatattgc gtgaactctt tttgagaact gccgatgtgt
     7141 cttcctttct tgtcaaagat aattgctcgt gagctagttg taccttggtc aatcgccatg
     7201 atatacgttg attctgtcat tgtgattccc tccattatat gcaaacgctt tctttaacta
     7261 ctcttatcat actactgttt taaaaaaaga aactgtcaaa attttattct ttttttcttt
     7321 ttttgacagt tttttattat gaagctattt tttaatcaat tcttttcttt tttcagcgtc
     7381 tctattcttc ttttcaatcg aataatgtcg taaggtgacg caaattctga cagataatat
     7441 tcacactgtt ttttgtttct ctggcaaaat gtgatcacca aatcatattc tttaccttct
     7501 tcataacttc caatctcaat accgccgatt cccctcagtt ctctgatcag atattgttga
     7561 aaagcaatac gataaatcgg cagactttct aaatcgattc caatcactac tggttttgca
     7621 atataaaaat cgatcatcaa aagaacattg gcatactgga tcatcaggta actaagctca
     7681 ctatcttcag ctcttttcct cggcaattgc tgcaccgctg tttcctgtaa tttcgccagt
     7741 aactgtgtta aggaatgccc gagcattttc tgctgctgtt ccaaaagccg atcacgctca
     7801 tagacttcga tctttccaac aaagaaaaac cattcattgt tgatctggga aatttggtag
     7861 cctactgcct tttcttcttt gattttcaaa cgacgaggac gataatgcca taacatcgtt
     7921 tcccgaatgt aagtatctaa aacagcggtt ggtaactttt tgctccgtgt caaatcatac
     7981 tgataaaata tttcttcgtc tactatcgaa aaagaaacaa aagagctgta aaacatcatt
     8041 gtttcgtata cattcagttc tgctgctgtt cgactaagat gcatcacgat gattggatcg
     8101 attgcttgat aaagccggtc cgactgatag agtaatttct tttctttcaa agttgcgtac
     8161 gtactttttt cattcaatag tctttttttc gtaatcccga gccagcaagc aatctgtgct
     8221 tctgctgatg ccgtaaacgt tgtttctaat acacggtcca atcctagaat gaacggtctt
     8281 ttttctggtg tattttgcaa aaaaagcggt cgctgatctt ccgggatata ttggaatagt
     8341 tcataataaa aatatcgtat ctgtagctct tctccgtata attgaccgtt tttgatttgt
     8401 aatccaaatt cctctaagag ctggttcaat tctttgatct ttcgaaaaac agaagactca
     8461 ctgatattaa acgctgtcgc taatcgaacg atggatactt gctgatgttc taataaatag
     8521 acgagcatct taaacttcaa gctttcctgc aataaaaaca atagaatttt ttccaaactt
     8581 acatcttcgt ccaacacaag cagcgcctga tactcatttc gctgtacttc tacttttttt
     8641 tccatcattc ggccaatcaa ttcaatatct tccaaatatt gatcaaacga ggcttttgac
     8701 agttggacat gctctctcaa accattgata gaggctgttc ccccttgcag aacaaggttt
     8761 cgcaaaatca ggatctgtaa ctgctctttt ctttctaata atgcttcaat cttcataggc
     8821 agtattcgcc acctttgacc acaccatatc tggcacacgg tattctggct ggaaaaatag
     8881 ctcatgttct tctaactgtt caaacgtcca tccttctttt actgcaattg tcagttggtc
     8941 aagaaggtgg aacatctctt ttttgctttg gagctgtgct ccaagaatct ttttttcaga
     9001 atcaaataga aatttgagtt taactttgct ttgatttatt gctgtaagct tgtagtctac
     9061 gtatcctgtt tcaatttttt ggggataaaa aatgctctct tcttctgtca aacctacgct
     9121 tcctaagaaa tagccaaacc actgattgcc gactggacga taggttccct gatcctttgt
     9181 ctgtgagcca ctaattgtct tgctgacatt tcgtgctgta cggacagcgt tatttactag
     9241 agaaacatac attttttcat ttgttggacg aaaagttact tggatcgcat cgccaatcgc
     9301 ataaacgtct ttttgagacg tttgcaagta gtcatctacc cagatcgttc catcatcatt
     9361 caatgttaat tgttcttccc acatctgatt gtcggggcga gaatgattag ccagcaaaac
     9421 aagatcatag ttctcctttc ttttttccgt ctctacaata gcggtttcat tttcggtcaa
     9481 agacacaact tgttcttcta aaaaaagtga aagcccttga tttttcattt cctttactaa
     9541 cggttctgtc atttcaggat ctagataacg aaaaagaatt gactttcggc tttcgtaaag
     9601 atggatttgt ttttttcttg atgccaaccc ttcagctatt tcgattcctg cttgtcctgc
     9661 accaataatt gcaattttct ttgcattcat gatcttttgc tgaagagccg ttacgttttg
     9721 tatcttttta actgggtgaa taagcgttga ttctgcagaa atatttcgaa acttttgatt
     9781 tgaaccagtc gctagaatca aacgatcaaa atccaatttt tttcctcctg ctaaaacgac
     9841 ttttttttct ttcaatccta ttactgtctt ttctgtgtaa acatggattc gatagcggtc
     9901 agtcagttct tctttcgtga tccaagaaag ttgtttttca ctggtaacct ttccttttaa
     9961 tatcaaattg aacccgctgg gaatccatcc taacttcgat tgcttttcaa tcaaatatat
    10021 ttcacttgtt ggcatcaatt ggcgaagata aatagccgca tgaatccctg caaacgaccc
    10081 gccgacaatt acaatgttca tctgcattcc tctttcttat aaggtgttct actgctaact
    10141 tctcaatcag aaaacacagt attcataact cgctttcaac cttatgatac atagcgatgc
    10201 agcagaatca accctctcca tcataccgtg ttctaggtct gcttctcaat aatgtactag
    10261 tggacctaat tgaagagtat catttctgaa gagatgtttc aaaaaatgta ctcttataaa
    10321 agaaaaaaac tcgcctttta cgtacctctg tccaaatagg aaacagaaaa gaacgaaaag
    10381 acgagtttta gcgtgtgtaa tgtaatagtt agaatagaca catcatttat ttttaattct
    10441 ttaatgcttc aacaactgct tcgcctaatt gatgagcaga ttgaggattt tgccctgtaa
    10501 gaagccgccc atcaatttga accacttctg aaaaagctgc tccagactga tagtttgctc
    10561 ctttttcttt caaagcatct tctgctaaga atggtacttc attcgttgta ccatttgctt
    10621 cctcttcttc attactgaac ccagtcactg tacgtccagc taaaatagat tttccagact
    10681 catctgtaac tggcaacaat cctacaacac cgtgacaaac agctgagacc agcccttcat
    10741 ttcgatagat ttgtgaagcg actttcccaa gatctattgc ttctggaaaa tcccacattg
    10801 ttccgtggcc accagcataa taaaccgctg cataatcatt tgcatccaca tcatcaggac
    10861 gtaaggtatt tcccaaggcc cgatttctga aatcttcatc ttcataatag gcccaatcga
    10921 tttcatccat attcaaaaca ctttgcggtt ctaaaggtac atatccgcct ttaggactga
    10981 caaaatctgc atcgatccca ttttttacta atacatcata aaaatgagtc agttcactta
    11041 accacaaacc tgtaggacgc tcgatctttt cgtacttttc tacatttgtt aatacgatca
    11101 atactttcat tttcccatcc tctttctata aaaattgcgt tattgataac gtgctttcaa
    11161 gaaattatat aatgggcttg gcaaataaag ctctttatat gcttcttcaa aaggtttcgt
    11221 tacaaattca tcctcacggt tttccaataa tttctctgcc cagtcaggat cgacgatcat
    11281 ttgctgtcca attgctgcaa gttctgcata tttcaatacc tgctctacat cttcgcgatt
    11341 gcggatccca ccgacaccaa ctaaagggat ctttccttga atcgtgttat ggaccacttc
    11401 aagaatcgat ttatcgttgt atttatcaga acgagctatt cgatcataca tatttaatga
    11461 aacatgcaga taatctagtt ttgactcacg taatcgttca agaagccaga tcgtgtcttc
    11521 aaaacggata cctggtgttt catattcttc tggtgagaaa cgatacccta cgatgaatgg
    11581 ttttttagca tattgttcaa ccgctgagaa aaccgttgtt aataattctt caataaaacg
    11641 ataacgattt tccaagctac cgccccattg atcctctcgt ctattcgaat gcggagagaa
    11701 aaattgttgg atcaaatacg tatttgctcc atgcaactcg attccatcaa atcctgattc
    11761 aatcgctcta cgtgtggctt tgccaaatgc ttggattgtt tcaacgattt cttcttcact
    11821 cattgctcgt ggtgtttccg catcaggacg ttttgctgca actgcacttg cagatacagg
    11881 ctgttctcca gataaagttg ctcgacttgt cattcgtcca ccgtggaaaa tctgaataat
    11941 cgcttttgcg ccttctcctt ggattgcttt cgctagctca cttaatcgag gaatcatttc
    12001 atcatgagcg atgctgagtt cacctggcca gccttttccg atatcttgga catttgcagc
    12061 accagtaata acagcaccaa gacctttaga acgttgttta taataagtga tctcatcttc
    12121 ggtgatcact ccattgaaaa agctgagttg tgtcgtcata ggcgacagca tcaatcggtt
    12181 cttcaattga actcctgaag gtaaagtaat tgattgatca aattgtgaca ttgtcattcc
    12241 tcccataatt cgctctgatt aacctatcga gaatcatcta tcattaaacc actcgattag
    12301 ttgtacatgc aactaatttg cttaaaaaat tttatttttt ttgtaactta tctgttgcat
    12361 cattcattgt ctgggctaat tctttacttg cttcatttcc caaaactgct ttgtatgact
    12421 gatgaagttc ttcccagcta ccatcgactt ccttagcaaa ttggcgtcct tgttctgtaa
    12481 gataaactaa tgacaagcga ccttcgctaa ctgttttgac gtatccttta tattctaatt
    12541 tttcaacaaa acgagcaatc gttgatgggg cggaatagag catttgtgca atatcttttt
    12601 gtggtattcc atctttttct tttaatatca ataaaataaa accataagtt ggtgccagac
    12661 ctgtccttct aaacgcttca tcagctaatt tattcaacat gcgatctagc tttttcacgc
    12721 taaagaaaag acattcatct aacgcttttt caattggctt ttccatttca tcccctccat
    12781 tggcgtacat atcatagtat gatttttatt ttttgtcaaa aaacacggaa taatgtccgc
    12841 ctcctctgtc tagatttgaa aaaaagaagt agccatcttc aaagaatttc ggcttgtttt
    12901 cgacaaaact ccttctggaa caccgtgaat cagaggttgt gaaaaagtcg tttagttttg
    12961 agtatgaaga aaaattttgt gaaaatggct ttccacattt ttgcataaat ttggcttaat
    13021 accgaaaaac tggcttttga acaccgttta tatcagaggt tgtgaaaaag ccgtcctgca
    13081 tcaagtaata agaacagcca aacaaaaata gttcctcata ttttgtcgtt ttttgagcta
    13141 atgccgatgg tcagctgatt gtacaccgtt tattgagagg tcgtgagcct gccgttgaac
    13201 tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gttttatgac tatggtgact
    13261 tgtgatcgag gagttggcag gagaatactg tttattcgta aagaaggggc tgtgacaaga
    13321 cttttgtcac agtcccttat ttccttttat tataggattt cgccatttga ttggatgact
    13381 tgtctgtacc aatcaaatga ttttttcttt aaccgtttct ttgtgccatt cccttcgtca
    13441 tcttgatcaa catagatata tccataacgt tttgacattt gtgaagtaga agcgctgacg
    13501 atatcaatgc agccccacga cgtataacca aataaatcta ctccatcttt gatagcttcc
    13561 ttcatctgct caatatgctg cttcaaatag tcaatgcggt aatcgtcttg aacggttcca
    13621 tcttcttcta ctttgtctat agctccgagt ccgttttcag ctatgaacag tggtttttga
    13681 tacctatcat ataattgatt caaagcaatt ctcaaaccct taggatcaat ctgccagccc
    13741 cattcacttg atggaagata cggattttta attcctccag ttattaagtt tccttggacc
    13801 ccttcccaat tttctggatg agcactgact gcactagaca tgtagtagct aaaagaaaca
    13861 aaatcaacag ggtaggctgc taagatttct tcatcaccta cttctatttt taagttgaca
    13921 cctttttctt caaacattcg ttgcgtatac gctggataat atcctcttgc ttgaacatca
    13981 ctaaagaaca aagaagttct ttgctgctca attgtttttt gaatatcatc cggatcacaa
    14041 gaatacggaa caagaagctg atagctgatc atacaaccga cttgtgaacc tttgataatt
    14101 tcatgacaag cttttgtagc taaagcactt gctataaact gatggtgtgc accttggtat
    14161 ttcgcttgtt ccaaatgggg atggttttct tcaattactc ctacgctaac aaaagggtga
    14221 tgttttacac agttgatctc attgaaagtc atccaatatt ttactttatt tttatagcgt
    14281 ttgaatactg tttcagcgaa acgaacgtaa aactgaataa gctttcgatt aggccatcca
    14341 ccatattctg taaccaaata taacggcatt tcataatggg aaagagaaac gactggctca
    14401 atcccatatt ttagacattc gtcaaacact tgatcataaa aaaataatcc cgcttcattg
    14461 ggtgtttctt catcaccatg aggaaaaatt cttgtccatg cgatagacat ccgatagcat
    14521 ttaaaaccca tttcagcaaa taaacggatg tcttctttaa agtgatgata gaaatcattt
    14581 ccgtgtcttt taggataata atacttgctg ttgggatcct gcgccgcttt tacttgctga
    14641 tgcgtcatca tatgccattg atctgtccat ttttctttag gtaaatcgct gtcgaatgta
    14701 tacacatcag acacagataa tcctttttca tctacatccc atgcaccttc acattgattg
    14761 gccgctgtcg ctccgcccca taaaaatcct tctggaaatt ttttcatatc gatcccccta
    14821 aacttgctag ttttcttgtt tgattatttt tcttatgact agtaatgata tttttgaatc
    14881 tactactatt tgtaacaata accattgtgg tcggatcata tccctctttg attaattctt
    14941 gcaaatctac ttccatcaat aaatcgcctt tacgtacttt ttttccatca gatactgcac
    15001 tttgaaagta tttgccattt aaattcactg tatcaatacc gatatggatc agtatctctg
    15061 ctccttcttc actgactacc ccaattgcat gtttcgttgg aaaaactgtt gaaacttccc
    15121 cgtttacaga actgtagatt tttccgtccg ttggaatgat cgcaaaccct tctcctaaag
    15181 atccactagc aaatgttgga tcgtttactt cagataaatc aattatttca cctgctgcag
    15241 gagtacttaa cactatatcc tttgaggatt cttcttcgat ttcatccttg tagccgaaaa
    15301 gaaaagttag catacaggaa ctgatcatcg ctaagaaata actagcaact agccaaacaa
    15361 atccttcacc aatataaatc gggaaagaca aaatacttct tgtagcaact gccaaagcac
    15421 cggctcctgc ggcaccagct actccaccaa atatagctcc acatatacaa gcaatgtaaa
    15481 atggtcgttt atatttaaga gtcacaccat aaacagcagg ttcagtgatt ccaaataatg
    15541 ctgtgataaa tgcagataaa gataaacttt ttactttctt atttttcgtt ttcaaaaata
    15601 ccccaagtac tgctcctgct tgcgcagcta cggcagtcat acatgctgga cctagcgtat
    15661 cacgaccgta gaaagaaaga ttgttggtca ttactggtac aagtccccaa tgtaacccaa
    15721 aaatcactaa cacttgccaa aatccgccga taacagcacc agataaaaca gggctaaagt
    15781 tataaacagc taaatatcca gctgcaatca gctgacttgc ataatctgca attggaccta
    15841 caactaataa aataattggg accatcacga tgatacaaat gaatggtgtc aataaatttc
    15901 gaatactttc gtgcaagctt ttgcgaatct taggctcaat ataagaaaga acccatacag
    15961 ctaaaattgc tggaataacc gtacttgtat accttgctag aatcacgggc atccctagaa
    16021 atcgcaaggt tatgctatca ctatatgcac tgacaatcgt aggataaacg agagctgctg
    16081 caataaccat agcgatgaaa cgatctgtat tgaacttttt tgcagcagta tatgccaaaa
    16141 atataggcaa gaaatagaaa aatgcatcag ctgctgcatt caaaatgata taagttcctg
    16201 attcagtagt taataaattc aaactcgtac ataaggcgag tactcctttg atcaaaccac
    16261 ttcctgccaa gacgcctaaa gtcggggcaa aaacagcggc gatcatgtct ataaaagagt
    16321 ttaaaatgcc ttttttctca gttcctctct ttccactaga tgcatcatct tctagtttca
    16381 tttgtttgga taatacgtca tacacttcat ttactgcatt tcctactacg acttgaaaca
    16441 ttcctccagc ttcgactaca gtgatcacac cttctagttg ctctaccttc tccttatctg
    16501 ccttcttatt atcttttagg gtaaagcgta accttgttgc acaatggtaa acattgctga
    16561 cattttcttc gcctccgaca aatcggacaa tatctttcgc taattgtgaa tagtccattt
    16621 ttacttcccc cttgttaatt aatgccgtag caatccttct tacaagataa gtatagttct
    16681 tagaaaacgg tttctccatt caattagtac cccttctaat agcgaacgaa actgtacaat
    16741 agtgtgtatg gtttacttag ccgagcaaat cactcactgc ttgaaagaaa acaagtatat
    16801 cgtttctctt atagtcaaag gagacaaaaa aaacacgctt gatcttcctt tgctgatgta
    16861 taagtggtat ctcaagctga tactctgtga tttttgtttc tacttggctt ttgataagac
    16921 agcacaacaa agtctcggca tttaacggaa gcataactag gtcagaacta ggagagatta
    16981 gttgctgaat ggaatctatc tttttatgga tttttatttg attttcttct ttgtagtaat
    17041 gatcaagcag ttttgttttc tttttttctt caactatggt cagcttttca cgcaaaatct
    17101 gataaatctc ccaagattca aactcatcta gaacactagg tagataaatc aaatcatccc
    17161 actcaaaaat aagctcttgc tcagtagtca aaattgcatc atattccgtt attttgtttg
    17221 attgattttt gaaaagatac actggttcta tccggcattc tttgatccac atgttcatac
    17281 tttgttgaat ctgttttttt agtgtattca aaatactaat tggctggtta ctaacaatca
    17341 gtatctcccc ttgttttcga aaaggattca acgcttcggc aagatacaaa acaaaataag
    17401 cgctttcagc taaattggga cgtaattgaa aatgtattgg gaagaaacgc cgaattaaat
    17461 aaatttctaa aggataacgg gagatgatct tttgtacaaa atgattcatg gtattatgtc
    17521 cggcatccat cctcgttttc atttgtttga tatgggttag caaaaattct gtttcatgga
    17581 acaataacga aacaggaaga tctaaaaaac gaataatcgt ttgctcaaat gctcttagat
    17641 tcttctctat ttctttgttt ttttcatcat taatcatcaa ataatttaac tctaatagtc
    17701 gattttcaat tgcttgtttt tcttgattgg aaaaagaata gggtagttct gatgataact
    17761 tttttgtgag ggtctctacc atcggaataa ataatgtata cctgtttgtt ttttctaaaa
    17821 gttcttctct tcgaagaatg gtcaaaacga gataccgaga aaatcgtatg aagtcctctc
    17881 ctgaaacaat ataattatat gatataagaa cctcttttaa tgtcttttgg acagtcggca
    17941 tgatattgga aaaaattgta ctgatttgtt gaggaaacct tgcgtgttgg atcaccgttt
    18001 ctgttccaac aagagataag aaaattcttt ttgaattttc ttcagcaatg atttttagtc
    18061 cttttgtcga cgaaacttcc agttctaatt taggattgcg ctcgatccaa cgcaaaatcg
    18121 tttttatttg taatgatacg gctgtttttg aaagaaagaa aatgtctgct atcttttgca
    18181 tactgacata ccctttttga aacaataagt acgtcacaat agttgaagct cggaaatcca
    18241 tgctggaata gattttatgt ttcgtcacct cttgtatgat ttgctgttta gtttcagatt
    18301 caattgattc taaaaaataa cctgaacgtt tatttgactg gataatgatt ttcttctcgt
    18361 agatagcaag catatttttg atttcagttt ggatcgtttt cttgttggta cctaaagctt
    18421 cagctaattc ttcgcttgtt atccaatgat tcttatttct taatactaca agaatttgga
    18481 gttgtcgttt atttaagtcc atttgcttac acctcctctt tgttataaac atacaggaaa
    18541 aacctctcaa atgacaatag tcacttgcag aggtttaaaa atcatttgaa gaaagatcta
    18601 tattcaaggt ttttaccatt agactggata atttgtttat aatattcaaa actatctttt
    18661 ttcaatctcc taaagcttcc gctcccatca ttttgtctat ccacataaat aaagccatat
    18721 cgcttcctca tttcccctgt tcctgccgat acgatatcga ttggtcccca ccataagtaa
    18781 ccaatgattt ggcaaccatc ttttattgcc tcagcaattt cctgaagatg tatttttaga
    18841 tactcacaac gttttggatc gtggattcga ttcaattcat ctggtgcttc atctagtcca
    18901 atgccatttt caactatgaa taatggtaat tcataacggt ctgtcaattc attcatgaca
    18961 taacgaaacc ctagaggatc aattggccaa ctccaaggtt cgggagattt ttccttcaaa
    19021 aagggattat cgaaaccatt tgctccgcca gtatctacgc gcatttctgc ttctttttta
    19081 tagacagcgc tacgataata actaaatgca atataatctg ccgtgttagc ttgcaagcac
    19141 tgttcgtctc catctaacat tactggtgca cagtcttctt ccttccatgt cctgtaaata
    19201 taagctggat aatgccctcg gcacatgaca tcggagaaaa accatgaatt gcgtttaaat
    19261 tgcagtgaac caaatacatt ctctggatca cagtcatatg cataagtcgc taatgcagaa
    19321 aagctacaca taatacccat cttagcattt ggatcgattt tctttaataa ttgagtagca
    19381 cgggcgttcg ctagaaacat atgatgacag gcttgataaa tcactttttt tgttagtgta
    19441 gcaatgggtt caagtttatc tctagcaggg aatgtaacca atccagcagc tgcataaggc
    19501 atacgaaaag cattattgat ctcattaaag gtcatccaat atttcacttt cccttggtag
    19561 cgttcaaaga cggtctgcgc ataaaattca aaaaagtcga ttaattttcg attttgccac
    19621 cccccatact ctcctaccaa tgctaagggg gtttcataat gactaagagt aattactggc
    19681 tcgatatcat atcgatttaa tgtattgatt agtctgtcat aaaatacgag accagcctca
    19741 tttggcttct gttcgtctcc tcttggaaat attcgtgccc atgagatgct tgttcgaaaa
    19801 gcttttaaac ctaactcctt cagtaaacga atatcctctt caaatcgatg ataaaaatca
    19861 atcgcttcat gagaaagata atggagtgat tcgtctaaag caatctccca aatctttttt
    19921 tcttcattcc actgaatact aggatgctgg ttcatgatcc cgataagcgt atctgtcaca
    19981 ttaggtaatt tgccgtcttc caaccatgca ccttcacatt gattagcagc tactgctccg
    20041 ccccataaaa aattttctgg aaatatttcc tgatccataa ctatctctcc tcgctatcaa
    20101 caatttctta cttgaagtat agctcgattc atcaaaacaa gatattctaa ctatttcggc
    20161 agaagagaag aaaaaaattg aatgctactc ttcattttta atatttatat ataacaaata
    20221 cataagacga tccccgaaag aaaacactcg ttaaccatct aagcaaacga tttattttac
    20281 ttccaacaca aatgcttcat aaggtcgtaa atcaactttg ccgtctggta actgtttcag
    20341 aggataattt gacacaataa cccttaaagc ttcacgtgga atagtgaacg tctgcttttc
    20401 actggacata ttacaaataa cgagccattg ctggtcttca tatatgcgag aataggcaaa
    20461 gacatgctca ggcgtctttt ccactaattc atagtctccc cagacgatga tgtcatgttt
    20521 tctacgcaaa tcgatcaatt tcttgtacat ataaaaaaca gaatcttgat ctgctaacgc
    20581 ttcttctgca ttgatttctt tataatttgg attgactcga agccatggaa ttccagtagt
    20641 aaacccagca tgttcacggg cgttccattg cataggcgtc ctagcattgt ctcttccttt
    20701 cgccttgatt gattccaaga tttcctcttt tgtaaatcca gaagaaatcc tttcgttata
    20761 catattgatt gtctcaatat cttttgcttc cgcgatatca tcgattgggc aattcgtcat
    20821 gcctaactct tctccttgat agatataagg ggtgcctttc atcatatgga gcaagatagc
    20881 aaacatcttg gcactttctt ttcgatacac atcgtcattt ccccaacgag aaacaattcg
    20941 cggaagatcg tgattattcc aaaataaact attccatcct tcatcaccta aagatgtttg
    21001 ccatttggat aatacttttt tcagttcaga gatactcaag ggtttcaaat cccatttgtc
    21061 tttgccttct tgctgatcta atcctatgtg ttcgaattgg aagaccatgg ataattcatt
    21121 tcttttagga ttggaataaa gtttagcgat ttcggtagtt gctccccatg tttcgccaac
    21181 agtcatcaga tttttgtcac caaaagttgc ttgattcatt tcctgaagat actcatgcag
    21241 tttaggtcca ttacttgtga tttctttatc cggttctttg ccaatcatat cgatcacgtc
    21301 catacgaaag cccccaatac ctttatcgat ccagaaattc atcatttgat aaatctcttg
    21361 gcgaacctgt ttatttttcc aattcaaatc aggttgacgc ttgctataca agtgcagaaa
    21421 atattgcccc gttttttcat ccaactgcca agcagaacca ctgaatattg agcgcaatct
    21481 attaggctct tttccattga ccggatcacg ccaaatataa tagtcccgat aaggattttc
    21541 tttattttta cgggcctcaa tgaaccacgg atgttcatca gaagtatgat tcaccaccag
    21601 atccatgatg atacggatat ttcttttctt tgcttcttca atcagttctt ccatttcttc
    21661 catcgtaccg aattctgcca tgattgcttg ataattactg atatcatacc cattgtcatc
    21721 attcggagac tcgtatactg gagaaagcca aattgcatca atacctaaat acgccagata
    21781 atccaacctg gaaataatcc cccgcaaatc tccaatacca tcttgatttg aatcttgaaa
    21841 acttcttgga tagatttgat aaacaactga atggtgccac catttttgtt ccatgatttt
    21901 cctcctataa atggatgttt ctattttttg cttaatcatt tattttagtc tctctcacta
    21961 aaaattgtaa cgctttcaaa tagctattgc accttgttat cggtaacaga ataaaacaaa
    22021 aaaacagctg aggaaattcc ctcaactgtt ttttgaaagg attgatttat gcagggaaga
    22081 atgctgattt cacaacttct ttataaacgg tgttcactca gctgtccctt ggtattagct
    22141 caaaaaacgg caaaatataa gaaactattt tttcgttttt ttctctaata ctcaggccag
    22201 aacgctgatt tcacaacctc tttatttcac attcaatatc cacatgaatc ccacaaatac
    22261 aaagaacaga ccatacatga ttggagatac ttcttttcct cttttagctg ctaacatggt
    22321 aattggatag aagataaaac ctaatgcaat cccatctgaa atactataag tcaaaggcat
    22381 gccgatcaag atcaagaatg aagggatcgc aatctctaag tttccccatt cgatttgtcg
    22441 caaagattgt gccatcaata ctcctaccat aatcaatgct ggtgcagtta cttgtgaagt
    22501 aacaactgat agtaatggag aaaagaacaa gcctaacaag aagaaaatcc ctgtagtaat
    22561 agctgtgata cctgaacgtc ctccaacggc gataccagct gatgattcta caaatgcacc
    22621 aacaggagaa gtaccaaata aggaacccgc aagcatagct gttgaatcag aagctaaagc
    22681 tttccctaca cgaggcatct tgttgtcttt catgaaacct gcttggtttg ctaatccaac
    22741 taaagttcct gctgtatcaa agaaagtcac aagcaagaat gtcagcacta cgacccacat
    22801 ttgaagagaa ttgatgtccc ctacatgttt taatgcaact aaaaatgttg gtttcaaact
    22861 tggtgctgcg gcaacaattt cagaaggcat tggaatcaaa cctgtgacca gacctaaaat
    22921 cgttgtagct gccatcccga tgaagatgcc acctggtaca cgccgaacaa gcaagatagc
    22981 agttacgact aatccaaaaa tcgtcaacca agtagaacca acgttgagtg atcctaatcc
    23041 tactaatgta gattcattcg aaacgatgat cccaccttcg ctcagaccaa ggaaagcgat
    23101 aaatagaccg ataccgccag aaatcgcata tttcaaatct gctgggatcg catcaatgat
    23161 catttcccgc agcttgaaga ttgtgatcaa aataaaaatc aaagaagcaa cgaatacgcc
    23221 ggctaatgct gtttcccatg gaatccccat tcctaaacaa acagaataag caaaaaaagc
    23281 attgatccca agagcaggtg ccgttccaat tggataacgg gctaataccc ccattaaaac
    23341 acagccgatc gcactggcta atgcagtcgc agtaaacact gctccttcat ccattccaga
    23401 agcacctaat acagttggat ttacaaataa gatataagcc attgagataa atgttgtaaa
    23461 tcctgccagt acctcacgtt ttaccgtggt gttcaatttt tctatttcga agtaggaaag
    23521 tattttttcc ttcatcttcc atccccctaa atccgaacat tataaataaa gtccgttaaa
    23581 atttcgattt attaataaaa caacgtgttt tatcatatac taaaacaaaa cataagtaaa
    23641 caaaaaattc ggattttaag caaaatccga acgttccatc tgattaaatc cctttatctc
    23701 aaagttcatg aacattcact ttggattaca agaatttctt tattgtgtta ataaagtttc
    23761 ttcattctag tctcaaaaat caatagtgtg atttgatttt accatttcgt ttctgttctt
    23821 ctttgatgaa ttcttttgta tcgacaggat tcagattatt tagtccgcca aaacctgtat
    23881 cgaccatttt tccccagcct tttgtcttct tttttcctga tttgagattc tttgctcgaa
    23941 gcttctcact atgttttgct cgccgatact tcaaataacc ttctaatctt cccattagat
    24001 ccactgtttt tcctcctttt tattcaatca gacgactcag cttttcctat aacaaaataa
    24061 ttgccttcag ggtcagaaaa attgaaagtc aagatgccat tgttatctgt gatttctcct
    24121 ggttcttcca tgttctcgta gatagtttca aattcatccg taaaaaatac taaagaaggc
    24181 ggtggtccta atacctctgg tgaaaacttc tgcacaaata cttttgggaa tacgcctaat
    24241 tctaattctt ttgagatgct gagtacaata ctcttaaatt cttctggcaa ttcgattgtt
    24301 tcagaaattt ctgcaccaat tttttctaca aagaatcggc aaatcatctc tacgtcattg
    24361 acataaagca tcacacgtgt tttttgtatc atcatttgac ccttctttct atttgatttt
    24421 ctttcctgtc tataaactga ttccattata tcacgaaagc gcaaacacct tgtgtttgat
    24481 actattcaag aaaaataaag caatagtttc taggattgct ttcctccaca aacttttttt
    24541 acaaaaaaaa aacggcaatg aatgccgctc ttcttatttc gattctttta taattctgct
    24601 tctgattcat ctttggttag tgtgtgtcta acactagtta cgccaaatgt ttgatcgatt
    24661 ttttccttca acgcttcgat tgcctgtgcg ccacgtgccg cttgttcagc attgatggat
    24721 tcaatgacca gcaccgctgc ttgtgtttct tccgtcagca tcgttctttg ggcttcacgc
    24781 cagttgagac agcgacagat cgctccttgt tcatcatagt agatgatttc ttccggaagt
    24841 gccggagcat cggaatctgc acctaaaggc aggaatgatt cgccaccttg ggcttttcct
    24901 aaatgcagat ttcctgcgat cttttcgata tcttctccgc cgcaaggtac gccaaaagac
    24961 aaggaaatac tattatagat atctacaagc ggattgattg ggaaaaattc ccgttcttga
    25021 tttgcacgtt tcagtaaagc ttcaatagaa gaacgtgcac cttttttagt cttgaatgtg
    25081 gtaaatgcct gacgccactg agcaatgact tcgttttggc tgaatgtttc ttctgtaagg
    25141 taatttcttg cttcttttgc tgcctcattc aataattgat gatacgtttc ttcactttct
    25201 tttgttacgt ggttatcgat accatttacg gataagattt cgatggttgc ttcaggaaat
    25261 acttcccaga aattggtatc cactattact tttttcatct gttcacacct cttgattaga
    25321 ataattgctt gatgaataga tagtctgttt ggggatcatc tcccatataa aaaacatgtt
    25381 cgctgtattt gataaatcct tttttacgat aaaaagcgag cgcaacatcg ttgtgttccc
    25441 aaacaccgag ccaaatggct gttttttgct tttcttttgc tttttcaata gctgtatcta
    25501 gtaaaaatgt accgatacct tgatgtttaa atttttttcg tacatagatt cgctcgattt
    25561 ctaaaaaatg atcagccatt tcctcagatt gactcgttcc tgtatttagt ttcaggtaac
    25621 ccgcaggctc ttgatcgata ctcactagga aaaaatcggt ttctttatct tttagctctt
    25681 ctgtaagttt ttctagtgca taagcttgtg tcaagtaatc agctaaatgc tccggtgaat
    25741 tatcttttcc aaatgtatct gtaaatgttt cgatactaat ttgttgtagt aagtctacat
    25801 ctgaaggcgt catttttcga atttcgtatt tattcacggc ttcttccttt caatagatcc
    25861 ttttattgcc ttttttgaca aaattccaat cttcgctaac attcttgcat actttattta
    25921 aaagatattc caattgtttt gcttcttcgt cagaaagacc atttaatgcg actgcattgg
    25981 agtagttgtt ttctcgaatg atgaaaggat agatttctgc tcctttttta gtcggataga
    26041 gtttcttgat ttttttattt tctttatctt ccaaacgttc gatcatccca ttggattcta
    26101 atttcttgat tgctcgggca gtcgttgtac gatctacttt gatcatttct gctagttttt
    26161 cttggatgat tccaggattt tcgcaaatac gtactaaata aagatactgt cctcttgtta
    26221 aatcgacttc tttgaattcg atattgctaa tagaatctag cgctcgggca atcattccga
    26281 tgtctcgaag gatttcagcc atttcatccc acctttattt tgtttgtaag actagttcac
    26341 ctctttttgt tgcaaatgca actattttca ataaaaaaat caagttcttt tttaataact
    26401 agtaaagtat attcttttct tcttttacga aaaagctcaa tcacaataga tctggttttt
    26461 gttttctaaa ataagtaact gtttcttcgt tgttcgcttt tctgatagct ttggcaactc
    26521 ttctatacta aaaaatccca tgttggaagt ttcattattc tcaataaact tggcctcttc
    26581 accgtggatc gcacaagcaa aaacaagttt ataatactga aaaagctgtg gaatatcttt
    26641 ttgtttattc gtatcaaaaa ctgctcgcaa ctgagtagtt tctactgtta atcctgtttc
    26701 ttcatagact tctttacgca cattttcttc aggagacaat cctatttcag caaatccacc
    26761 aggaagtgac cattctttcg tccgtttatc ttcgattagt aggaccttgc catctttttt
    26821 gatataggca cgaacatcta cttttggtgt tggatgtcct tctccaatcg ttgtgagcgc
    26881 ttttatttct tcgaaagttt cttttcctat ttcactgatc agttccaatg aaatcgtccg
    26941 gatctcctca aaacgctctt tatcaaatac atcttttcca taatatagac cagcttgaga
    27001 aagtgcgagt agtcttttgt attgagccaa cttcttgttc attctcttcc cttctttttt
    27061 agcaaatgta tgactatgta atcaagtata gaagactggt ttaattacgg caaataaata
    27121 taatgaaatc tgcaataaat aaaagaatgt taaaaaactc gagaacaaaa gacattcgac
    27181 ttctctgggg ctgtgaataa atggcgttca aaagcttgca tctgcggtaa taagccccac
    27241 caaacgaaaa atatgacgag ctattcttgt ctggctgttc ttattaattg aggcaggaca
    27301 gctttttcac aacctcccca ctaaacggtg ttctcctacc aactcctcgg tcacaagtca
    27361 caatattcat caaacatgag aagctgtttt tttgaaattg ctccttgtga ctcggagctg
    27421 aacgacaggc tcacgacctc tcggtaaaca gcgttcaaaa gccaattttt cgatattaag
    27481 ccatttcttt gttacttatt gtcattgatt tctttttcta atttatccaa tagtggagtg
    27541 taatccactt gagtatagtc tgttacatca aattcatata gtgttcctaa ctggaatttg
    27601 tcatattttc ggtcggcgat gatctttttg aatgcttctt tcgtaatatg attggtccct
    27661 ttcgttcgat cttccaatac gtcaccatag tgataatgat ccatgatata gctcaaatga
    27721 cgatctgatt cacgatcacg tttgtaccga cgttgccata acacttcaaa gtctgctgtt
    27781 aggcgaatcg tgatcacctg atatccgtat ctatctgcta attcttggag tcgtgctttt
    27841 tgcttatcac taaaaggata ctcggacaca atcactttct ttcctgcttc catatacaaa
    27901 tcaagtgctt tgtaatagaa aggataaact ttcgtccttt cttgttccgc ccgttcttcc
    27961 aaattgttaa acccgatgga ttctgcatag tattccttga tttcatcgag cgtgatggca
    28021 tacatatctg gaaatcgttc ccgtagttta ttcataagat atgtttttcc tgtaccagga
    28081 ctaccagcca gaagaatgaa atactttttc atctcattta tttcctaagt taattggact
    28141 ttccaagttc ataaacagtc caatcttatg cttgacaatt tcttttactg cttcatttgc
    28201 tggaatcaca ttatggcgaa gagacttgtt gatttctgtt tcactaaagg tttctttagc
    28261 tactttcgtc cagttgacta aaagctccgt tccaacattg aatttgcgga tcccgttatt
    28321 gatcaattca ggataatctt cttctttcac acctgttcct ccatggataa ctagggggac
    28381 atcaactact gcatcgattt ctttcagcaa gggaatgttt acttctgttt ttgatttgaa
    28441 ttgtccatga ttcgtaccga tagcaatagc taatgcatcc acgcctgtag cttctacaaa
    28501 tttcactgcg tcttccggtc ttgtatatac cttgtcattt tcagcgacat ggattccttc
    28561 ttctgtaccg ccgattgttc caagttctcc ttctacggaa acaccgtgcg catgggcata
    28621 ttctactact gcttttgtct tcaggatatt ttctttgaaa ggcagatggg acccatcata
    28681 catgacagaa gtatatccgc tgtcgattgc ttctttgata tcatcaaagt ctcgggcgtg
    28741 gtctaagtgc aagactgcat caaccatttc ttcttctgca atagctgtca ttacattgac
    28801 taacacatgc atgccgatat atttagcggt atcgacactt gtttgaataa tgatcggcga
    28861 acccatttct tttgctgcgc ctagcatagc aggaagcatt tctaggttat gcgtattaaa
    28921 agctccaact gtaaaattca actcttctgc tgtttttgtt acttctttca atgttgtata
    28981 catcttttat tgccacctta tctttttatt tgtttccttt gatcgttact tgttttgcaa
    29041 tttgtcgcat ctcatccatt ttgcccatgt agtaatcgat cccttgatca tcaccagcac
    29101 gagcagcatc tgcacgcttc tggttataga ggctcatcag ttttttatac ccatctccga
    29161 tcgtctgttt attttccaaa ctcttttcta aatattggat ggcttgatcg ttcacataca
    29221 attcagaaaa tttaagtcca acttgttcat aaagtttcgc taatttatgc ttatcttggg
    29281 tagtggaaat ctcttgtttc aattgtgtaa tctctgattg aatcttttcg gtcgcttctg
    29341 gcgatagggc tttttcttct tttgccagaa cttttttcgg ttctttttct tttttcttaa
    29401 aaaatccaaa catcattttc cctactttca attaaaatgc tttcaaaact gggcttaaga
    29461 tccaggcata taagagtgct gcggctactg tatctacatc ggttgctgta gcattgttga
    29521 atcccatcga attaaagatc gttactagca aagcgggcaa taatgtaata aagaaaccat
    29581 gagcaatacc gccaatgatc gctcctcggc gtccgccaac tgcgttgcca aatatccctg
    29641 ctgtccctcc ggcaaagaaa ttggttagca taccaggtaa aatcatcgct aagccgaaca
    29701 tgggcaaagt aaacatggca ataatcgtac caatcgttgt cgtaatgaaa cctaaaatga
    29761 cagcatttgg gctataagga aagaaaactg gacaatctaa ggcagggatc gcatcaggaa
    29821 ctaatttcat agctatccct cgaaatgccg gcacgatttc acctaacagt agacgtacac
    29881 cagctagcaa tacatacaca cccactacga attggatggc ttgtaaaaag gcaaacatga
    29941 tatagttttg atctcctgat aattcagaag cgaatggttc gcctgcaaat aaaacagtga
    30001 tgatatatag cgggaccatc acgaccatga cagacaaata agtatcttgc agaaattcga
    30061 aatgtgtcgg caacttgata tcttcgatcg actttttatt ttcgcctttc tcaccaaaaa
    30121 gcttagcaac accggcttca aataagtacc cgatcgtaca aaaatgacct aaagcaatat
    30181 catttgatcc agtgatttta cgaatgatcg gttgcgctac tgccggcata gcaatggcga
    30241 aacaagctcc gataaaacca ccgaccaaga tcaatacgat ccctcgcaga ccggcaaaat
    30301 agccaaagac tgtcgtcatc gttgccatcc ataaaattgc ttgaccagtt aaaaaaatgt
    30361 acttccattt tgtgaaacga gccaaaataa tattgaatac aaaaatggct aagaacgtca
    30421 gtgcaatatc tctacctaaa cccaaatcat tcattgcttg accattgata gattcaatag
    30481 aagggatgat cccttgcata tgaaatcctt cagtaaagat tttcccgaaa taagttaaac
    30541 ttccaacgat gatgctcgaa ccggcactca acacttgaaa tcctaggagt gtctttaacg
    30601 tgccagagat gacttgtcct gctgatttct tctgcagtag tagacccaac atagcaatca
    30661 atgcaatggt gatcgatgcc tgagtcaaaa tattttcgat gataaaattg ataacgccca
    30721 t
//
LOCUS       DACEPD010000026        30642 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.026, whole genome shotgun sequence.
ACCESSION   DACEPD010000026 DACEPD010000000
VERSION     DACEPD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.026"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(311..2386)
                     /locus_tag="IW797_001132"
     CDS             complement(311..2386)
                     /locus_tag="IW797_001132"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ1223919.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDYEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTYHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDADRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2388..3305)
                     /gene="glyQ"
                     /locus_tag="IW797_001133"
     CDS             complement(2388..3305)
                     /gene="glyQ"
                     /locus_tag="IW797_001133"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1223920.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(3691..4503)
                     /gene="recO"
                     /locus_tag="IW797_001134"
     CDS             complement(3691..4503)
                     /gene="recO"
                     /locus_tag="IW797_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ1223921.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKDMSEKDLPKKEE"
     gene            complement(4646..5545)
                     /locus_tag="IW797_001135"
     CDS             complement(4646..5545)
                     /locus_tag="IW797_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ1223922.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5560..5961)
                     /locus_tag="IW797_001136"
     CDS             complement(5560..5961)
                     /locus_tag="IW797_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ1223923.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIVGLILFVPKIYHLLFN"
     gene            complement(5939..6415)
                     /gene="ybeY"
                     /locus_tag="IW797_001137"
     CDS             complement(5939..6415)
                     /gene="ybeY"
                     /locus_tag="IW797_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ1223924.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6444..8636)
                     /locus_tag="IW797_001138"
     CDS             complement(6444..8636)
                     /locus_tag="IW797_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ1223925.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLFAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8660..9631)
                     /locus_tag="IW797_001139"
     CDS             complement(8660..9631)
                     /locus_tag="IW797_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ1223926.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10337..11716
                     /gene="brnQ"
                     /locus_tag="IW797_001140"
     CDS             10337..11716
                     /gene="brnQ"
                     /locus_tag="IW797_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ1223927.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGTNWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11872..12318)
                     /locus_tag="IW797_001141"
     CDS             complement(11872..12318)
                     /locus_tag="IW797_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ1223928.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12345..12521)
                     /locus_tag="IW797_001142"
     CDS             complement(12345..12521)
                     /locus_tag="IW797_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ1223929.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12683..13111)
                     /locus_tag="IW797_001143"
     CDS             complement(12683..13111)
                     /locus_tag="IW797_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1223930.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13203..14063
                     /locus_tag="IW797_001144"
     CDS             13203..14063
                     /locus_tag="IW797_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ1223931.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(14158..14451)
                     /locus_tag="IW797_001145"
     CDS             complement(14158..14451)
                     /locus_tag="IW797_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ1223932.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(14524..16251)
                     /locus_tag="IW797_001146"
     CDS             complement(14524..16251)
                     /locus_tag="IW797_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1223933.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            complement(16465..17391)
                     /gene="trxB"
                     /locus_tag="IW797_001147"
     CDS             complement(16465..17391)
                     /gene="trxB"
                     /locus_tag="IW797_001147"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ1223934.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17536..18969)
                     /gene="ascB"
                     /locus_tag="IW797_001148"
     CDS             complement(17536..18969)
                     /gene="ascB"
                     /locus_tag="IW797_001148"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1223935.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(18969..20372)
                     /locus_tag="IW797_001149"
     CDS             complement(18969..20372)
                     /locus_tag="IW797_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ1223936.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20602..21504
                     /locus_tag="IW797_001150"
     CDS             20602..21504
                     /locus_tag="IW797_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1223937.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21545..23431)
                     /locus_tag="IW797_001151"
     CDS             complement(21545..23431)
                     /locus_tag="IW797_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1223938.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIRAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILMMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGNLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKTSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFKSL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23527..24378)
                     /locus_tag="IW797_001152"
     CDS             complement(23527..24378)
                     /locus_tag="IW797_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1223939.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSEQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGEKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(24835..26619)
                     /locus_tag="IW797_001153"
     CDS             complement(24835..26619)
                     /locus_tag="IW797_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1223940.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQTITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26619..28370)
                     /locus_tag="IW797_001154"
     CDS             complement(26619..28370)
                     /locus_tag="IW797_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1223941.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFVIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(28512..28736)
                     /locus_tag="IW797_001155"
     CDS             complement(28512..28736)
                     /locus_tag="IW797_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ1223942.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            28987..30573
                     /locus_tag="IW797_001156"
     CDS             28987..30573
                     /locus_tag="IW797_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ1223943.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8541 a         6591 c         5458 g        10052 t
ORIGIN      
        1 taaaaaaaca taattaaact aatatattca cagaaatatt tgtaaacaag ttttccctat
       61 ataatcatac cacaaactgc tttcttgact agggaaattt acgatttaaa agtaaataaa
      121 acattaataa gataacaatt aatcgatttt acttttattt ccttaatttt cataggtgtt
      181 tttaaaagtt acacaaaaaa taaaataaca aaaaaaatta acattgaatt aataatagtt
      241 atttataaca aataaaccag agaaatagcc tattttgtgc ctgttctctg gtttatttac
      301 atattagaaa ttattttgtg atgatttgat ctaaacttgc tagtgacaaa gccatggcag
      361 caatttgact taattgcgtt aatcggttct tacgtactgc tggttcttct gccatgacca
      421 tcgtctgatc aaaataattt tcgatcagtg ggcgaaggct tacaagctga tcataatttt
      481 cgttgatcgt ttggtcagca aagcttgttt tcattgcctc tactgctttg tgcagttctt
      541 tctccgcttc gttttcgaac agttcagggt ctacttctga ttgtttttca agctccgctt
      601 ttttcgctaa gttcacgaca cgtgtcaaag cttccatcga tggtttgaag tcttcatctg
      661 caagatgctg cttgaacaat tgtgctgctg caaatacttt tgtcagatct ttttgttctg
      721 cattcaaaac agcttcgatg acatcatgac gaatattctt cgttgtcaat aattggcgca
      781 gacggccttt gataaagtcg atcacctgtt gttgtcccgc tgacaagcca atgccaaaac
      841 gatccgcatc tttactgata acttcactaa tcgtttcttg cagaacagat aaaggaaagg
      901 cccatttttt cgattcgatg attcgaacga caccatatgc ttgacggcgc aaagcatatg
      961 gatcgtttga gccacttggg atcatcccaa ctgcaaagaa agaaaagata ctatctaatt
     1021 tatcagcaat cgctaaaaca gaacctactg tagtctcagg tagatctcct tcactggata
     1081 taggcatgta atgttcacgg attgctgttg ctacggctgg tttctcgcct ttaagcagag
     1141 catatttttc tcccatgatc ccttgtagtt cagggaattc tcctaccatg ttggttacta
     1201 gatcaaattt atagatttca gcagcgcgtt gcagatccat caattcttcc tcagatagtc
     1261 ctacttgctc gcctatgatg cgggcgatga cttgtacccg ttgcattttc tcgtagatgc
     1321 taccgatttt ttcatggtaa gtaacagttt ttaatttttc aacgtattgg tcgattgaag
     1381 ctttcttgtc ttcattataa aagaattccg catcttctaa acgagcagtc aatacttttt
     1441 gattgcctcg aaccacattt tccaatttta cccgatcacc attacgagca gaaataaaat
     1501 gaggaagcag cataccttga tcattccgta cctcgaaata acgttgatgt tctttcatag
     1561 aagtgactag tacttcttca ggaacagaaa gatatttttc ttcgtagtcg ccagaaaaga
     1621 cagttggata ttcgactaaa ttgttgactt cttctagtaa atcttcgtct agatcaataa
     1681 cccagtgatt tttttctgct aattcgtttg cttgtgtaac gatcatttct tgtctttttt
     1741 gcggagacgc aatgacgaat tgttcattca atttttcttc atattctttt gcatgcgcaa
     1801 atgtcacatc ttcgcctaag aaacgatgac ctcgagaggc tctgcctgtt ttgacatcca
     1861 agatttcgaa tgggattact tcatcatcta acaatgccac gatccagtga atagggcgga
     1921 tatattcaaa atcgtattct gcccaatgca tcgttacagg gaaagtcagg cttttaatga
     1981 catctgctaa acctgtcaat acttctttgg attccttacc atgagtgtat ttcgttacat
     2041 agacatattc cacacctttt aattctctaa aagtgatagc gtctgtcgtc agaccttgtc
     2101 cgcggacaaa tccttccgct gctttcgtcc agtttccatc tgcgtccaaa gcaatttttt
     2161 tggctggtcc tttgacttct tcttcactgt ccgcttgtcg ttctggaata tttgttactt
     2221 tgattgctaa acggcgcggt gtggaaaatg tttcgatcgt atcataagtc aattggtttt
     2281 catctaaaaa ttttgctgtt ttttcttcta attgtttcat gcttggtgtg acaacatggg
     2341 caggcatttc ttccagcccg atttcaagta ataagtcttt tgccatctta ttcagcctcc
     2401 ttcacggttt ctggatttgc tgtgttttta ttcaacaaag ggaatcctaa tttttcgcgt
     2461 tctgccacaa agattttggc tacagcacga gccatgttgc ggatacggga aagataaccg
     2521 gcacgctctg ttacggatac tgcaccgcga gcatctaata aattgaatgt atggctgcat
     2581 ttcaaaatat aatcatacgc tggatgaaca agattttcat cgatacagcg tttagcttct
     2641 ttttcaaaac gatcaaagtt gcttagtagc aagtcttgat cgcttacttc aaaagaatat
     2701 ttggaatgtt catattctgg ttgtttgaag atctcgccat atttcacgcc atcagcccat
     2761 tctaaatcat agacactttc aacttcttgg atataagaag caagacgttc tagtccataa
     2821 gtgatctcag aagtgactgg tttacaaggc agtccaccaa cttgctggaa gtacgtgaat
     2881 tgtgtgattt ccattccgtc aagccatact tcccaaccca caccggcaca gcccatggat
     2941 gggttctccc agttgtcttc aacaaaacgg atgtcgtgtt ctaatggatc gattcctaaa
     3001 aggcgcaagc tttctaaata aagctcttgg atattctcag gtgaaggttt catcacgact
     3061 tggaattgat gatgctgata caaacggttt gggttttcac cataacgtcc atcagctggt
     3121 cggcgagatg gttctacgta agcagcattc catggttctg gtccgattgc ccgtaaaaag
     3181 gtatatgggc tcatcgtacc tgctcctttt tctgtgtcat acgcttgcat cagcatgcag
     3241 ccgtttgatg accaaaattt ttgtaatgtt aaaatcatat cttggacggt caatttttta
     3301 ctcatctgtt ttgccccttt caattttaaa gcgttgctgt caaataagaa tacaggaaag
     3361 aaaacgacaa aaaatcccta tgtctgatta cagacatagg gacgaataat tcgcggttcc
     3421 accctacttc cgaataaaga ttcggcagct tcatttatcg tactcgcaag tgcctcttca
     3481 aaaatcgttt gggtccggct cccactctcc cgaactcgct aaaccacgtg ttttttactg
     3541 atcttggtca atgtacgtat tcattttgca gttttcttgt ttctataata gatcctctca
     3601 ctgaacgatc tatttgatct acggttaata atagacggaa ctccctgtgt ttgtcaaatg
     3661 gttttgattt tttgttgtat gtttcattta ctattcttct tttttaggta gatctttttc
     3721 agacatgtct ttctctggcg tctttaatac gtgtgcccaa gacttcattt gatcgatgaa
     3781 ctttttgctt ttcaaatgaa taccgacata ttcatcatac agctggtcga tgagttcacg
     3841 gattgcttgt ttggtttctt ctttcagatg gattccactg attttatcat aagaaatagc
     3901 tgaaaacatc cgaacgaaat aaacagctcg aggatctgca tgataacggt gatcgtccat
     3961 cgaccaatgt ctttcgcata tcacaccacc atatttggaa gaatagtcaa attttccttt
     4021 tgtttcccca catacgacgc agtgtgtcca gattggtgca attccgaagc gttgcagtat
     4081 ttgaacttca aagatattcg tgatgatttc tgcatcatct ccttgatcca tccgctgcag
     4141 agcttgttgc gtaaattgga ataaatgcgg gtcatattga tgatcttcga ttgctgcatc
     4201 tactagattt agcaaatagg taccataacc gctcaaaaag atatcttctt ggatgttccg
     4261 aaacgcatgg acgtctttga cactgcttaa aaacgacaaa ccttcacttc gaaaattacc
     4321 gatatagact gcttcggtaa aggggagcaa agccggagct aatggctgat tttgccgatg
     4381 gattcctttg atataaaaca tcattttgcc agaagattcc gtaaagattt tgactaattt
     4441 atctttttct ttgtaattac ggctagataa aatcaagcct ttcgattctg caagctggct
     4501 catattctcc ctcctaaaaa ataaatctgg aaaagcagat tctgccgatc cagattattt
     4561 atgtttcgat cttccgccgt ttttctcaac gtgcggattt ttatccagtt tatttctata
     4621 cttaacctat aagaagttca gaatatcagt attcatcttt tcggtagccg aagtcttgca
     4681 gatagacttt tttgtctcgc cagtcttttt ggactttgac ccaaagttct agaaatactt
     4741 tgtctcctaa aagattttcg atatcctttc ttgctttcgt tcctacttgc ttcagcattt
     4801 ttccgccttt gccgataatg attccttttt ggctatctct ttcgacgatg atcgtggcct
     4861 ggatatggat tttatcattc tcgtttcgct tcatcgaatc aacaacgact gcgacagaat
     4921 gaggcacttc gtctcgtgtt aaaaacaaga ctttctcacg gatcaattca gaaacgatga
     4981 agtattcagg atgatcagtc acttgatcat ctggaaaata ttgtgggccc tctggcattt
     5041 cactgactaa tacatccatc aaacgttcga cgttgtttcc ttctgtagca gaaattggaa
     5101 tcacttgtgc aaagggcatc tgcgttgaat aatcttcgat gattcccaac aactcgtctg
     5161 gatggatttt atcgatttta ttgataatca gataaacagg agaatgcacg ttttttagac
     5221 gttcaataat aaaatcgtct ccccggccgc gtttttgatc agcgctgatc ataaataaga
     5281 ccgcgtccac ttctttcaac gcactgtaag ctgcttctac cataaaatct cctaaacgat
     5341 gctttggttt atggattcct ggtgtgtcaa taaaaatcaa ctgagcttct ggtgtagtat
     5401 atactccttg gattttattt ctagttgtct gcgccttatc actcatgatg gcaatttttt
     5461 gacccacgat tcgattcaat aatgtggatt ttcctacatt cgggcggccg acgattgcga
     5521 caaaccctga tttatgttct ggcatatacg ttctcctttt taattaaaca acaaatgata
     5581 tattttaggg acaaataaaa tcaacccaac gatgacggca aaacaagcgg tcactaaaac
     5641 tgctccagct gccatatctt tgattttttt cccaatcgga tgaaaatgaa aatccgtaaa
     5701 catgtctacg acattttcaa aaacagtatt gatgatctct actatccata cgagaaaaac
     5761 agctaaaagc agccataacc attctatccg atcaagttgg aaaatcacgc ccataaacaa
     5821 agcgaggatt ccgaacgcaa catgtttcct catgttgcgt tcttcctcaa aaacagtttt
     5881 gactccttgg acggcaaatt ccacggacat caggaaatgt ttgtttttct ctacgttttt
     5941 atcttttaag tccataggca tctaagatct ctttctgtaa accaaacatt tctttctcat
     6001 cctctggtgt catatggtca tacccgttga tatgcaagaa tccatgaacc gctaaaaagc
     6061 caagttcccg atcatacgaa tggccgtaag cttctgcttg ctctgcagca cgttcagtag
     6121 agatcatgat gtctcccagt acacgaggca atggattctc tccatcttca aaaatcactg
     6181 ggatttcgtc ttcaccctct tcttccaagg caaaactgat gacgtctgtc ggtgcatctt
     6241 ttccacggta atcacgattg attacttgga tcgctgcgtt gtccatgaaa gtaacagaca
     6301 tttctgtttc ttctggcaac tctaaatatg atgctgcaaa ttgcaacaag tcgtcgattt
     6361 ctttcatttt ctcttctgag acttggtttg tttcatccat gaaggtaata tccattatac
     6421 atcgcttctt tctattcttc ttgctattct tcttgttctt cttttatctt ctcaacttcc
     6481 gatttcattt taggatattt gatccgagaa tggaaggtac tgcttaaacc gctgatcaat
     6541 gatttttcta ctttacggat ttcttttaac gtcaatccac tgtcatccaa ttgtccatcg
     6601 gaaatacgct cttctataag attatggaca aaactttcga ttttttcgct tgttggatgg
     6661 tccattgccc gtactgccgc ttcacaactg tcagctatat tgacgattcc tgcttcacgg
     6721 ctttgaggtt ttggtccagg ataacggaat tgctcttccg tgacttccgg gttgcgttcc
     6781 tttgccttaa cgtagaaata acgcatcagc gtggttccat gatgctgtct gcaaatatcg
     6841 atcaccattt gcggcatatt ataatcttcc aatattttgg caccatctgt cacatgtcca
     6901 aagataatat gtttactgtc ttctggcaat aagaaattgt gcggattttc cgctcctgaa
     6961 ggcaagttct caacgaaaaa gctggcgtgt ttgatcttgc cgatatcatg ataatagcaa
     7021 gctactcgag ttagtaaaga acgtccaccg atttctgcta cagcattagc acttagattg
     7081 gctaccatca tgctatgatg atacgtacct ggtgcttctt ctaacaactg tttcaataaa
     7141 gggtgattag gattgctgag ttcatttaat acgatcacac tatcatcatt gaccatcaat
     7201 tcaatatagg ggtgcagacc tatcgtcagc aagaatgaga acaatgcacc tgtgaatccg
     7261 cagactagcg ttaaccaagt cgttgcatcc ccaaagctca ttccttggta gataatcaaa
     7321 atgacatcca tcatcacagg gaaaataatg acccacatag cagcgaataa cccttgttct
     7381 gagatccgtt tgcgtctgac gatcgttgcc atgagaccag aaaatacata ggacatcaaa
     7441 ataactgtca gaaagttcgt gccaatcgaa tcataaaaaa tgaaaagtgc ggatactgct
     7501 tggaaaatac cagccatgat ccctgctctt cgattcaaga aaaagcttaa aacaagtgga
     7561 gcaaaagccg cggggtaaaa cagcggaata taagcagctt gttctgtttg gaagagttgg
     7621 aaaaatttca ttagtactac gctgactgac attgctgtca cgtagaaaag tacgtatttt
     7681 gttctttctc ctgcattttg aaattgaagt gaattataaa tcagtacacc gatttgaagc
     7741 aaaacagcta aaaccatggc aatgagtggg aaaatcgaag tcgtttgatt cgtcaatcct
     7801 agtaattcca attttttcat cgctgaagca tcaatttgat ttccttcgcg aacaatgatt
     7861 tctccttgga agatcatcac tggttgtaca gcttctcgtg cagattgttt taattcttct
     7921 gtctttttct cattcaatac atcattgact acaatgcctt gatccactaa gtagcggatc
     7981 atttgttgct gctctgttgt gacattcaaa tactgaatct gatcttctgc ttcttgttta
     8041 aaagcttcca attcgctttc acgaacttgt ttactcatct gttcgtcaat gagcttcaaa
     8101 ctttcatccc taaccgtgtg aatttccgtt tcagtcattt caaatatcgt cgaataaaaa
     8161 ttgttcggca gtttttggta aaacgcaacg ttttctgcat tcacgttttc aaaatttttc
     8221 ttcagtgtag caatccgctc ttctgtagta ggtttcggga tattttcatc ttccttcgcc
     8281 ttatctacct tgtcattata agatttgttg atggtatcat tcgtcttatc gatcaattcg
     8341 aataattgct tgatccgatt gttctggtca tctgccagat ctttttgata agtatattct
     8401 ggagtcactg cttctgctgc tagttttctt ctttgttcgg tttcttccgt gttctcgatc
     8461 gttttattcg cacggatact ctcttctgct acttggcctt ctctaaaatc attggccttt
     8521 tgtttgacac tactaaatgt aacgccgcaa acaatgataa aaaacaagaa cagaaggaaa
     8581 ggcaatgctg cctttccgag tttttcttgt agtttctgca gtgttttatt caacatgtgc
     8641 ttatcctcct aagcaaagct tattcttgat gaagatccgc cttttcataa gctttgatga
     8701 tttcggcaac tacagggtgc cgaacaacgt cacttgcttc aaaattaaca aaatcgattt
     8761 ttttgatttg ctttagtgtt cgttctgcat gaacaagacc acttgtcgtc cctcgaggca
     8821 agtctatctg acttgtatcc ccattgacaa tcatttttga ttgataaccg agacgggtca
     8881 agaacatttt catctgagcg actgtcgtgt tttgtgcttc gtccaagata acgaatgcat
     8941 catccaatgt ccgcccccgc atgtaagcta atggtgcgat ttcgattacg cctcgttcca
     9001 tcaatcggtt cgtatgatcc aatccaaaaa tttggtataa agcatcataa acaggccgca
     9061 gataaggatc cactttttct ttcagatccc ctggtagaaa gcctaggttt tcccctgcct
     9121 ctacagcggg ccgcgtcaaa atgatttttt gcacttcgcc tttttttaag gcagcaattg
     9181 ctaatactac ggcaagaaac gttttccctg ttccggctgg ccctacacca aatacgatgt
     9241 catgtttggc taccgattca acatatttct tttgaccgct gttttttacc cgtataggtt
     9301 ttccattacg gtctttgacg atctcttcct catacatctc aacaaaataa tctaatgtac
     9361 ctttttgccc cattttcaat gccgtcacaa catcgggcgt acttacgtgg attcctcgtt
     9421 tgatcaattc ctgtaatgtc cgaatgactg aggcagccaa ggaaatctgg gattgttcac
     9481 caatgatctg aatgatttct ccacgtgtat ggatggttgt ttccgttgtt tcctcgatca
     9541 gttttatatg cttgtcgtgt gcacctaaaa gcatgctgac atcatcagca gctgtcaatc
     9601 taatctctaa agaactagac tcttccgtca aaaatagaca tccccttttt ttcgattcat
     9661 tctttcctat tttaccatac tcagaagaaa ctatatagca aataagaaaa ggttgtaaaa
     9721 gagtcgttta accatacgta ccaagaaacg ttaaaaaata gccgtttcca ttcgctaaag
     9781 gtctcgtttt gataaaaaga atctgagaat gtaagttata tgaaagtgga gaacaaaata
     9841 ttccgtttca ttcagcaggt attctctagg tatttttctt gtttcaaaaa aagaaaacaa
     9901 aatagttaaa atattcttgc atttcaaaaa aaacaggtgt attattacgt caacaaataa
     9961 acatacagca aagcgtcgag gaaaacagta ggctattcaa cgatttattc ccagtgagtc
    10021 atgtaagtga gaaatgacaa taaatcgaat agacgaacat catttccgag tcaatgtcga
    10081 aaacgtagat cattgtggct gcctccatta tcaggctagc ggataacttt atcaaaaagc
    10141 gtccgtgaat gaagaagctt ttttaagcga aaaaaggtgg taccgttcta gaatctagag
    10201 cccttttccc aagtggaaaa gggctttttt tatattttaa ggtggtggtt ttcatggaat
    10261 ctgatattaa tggcattact aattgctgca acgtttatat cgtcaatcaa aatgataatt
    10321 attggaggaa aaatttatgg aagaaacaaa aacttattca tggaaacaac tattgatggt
    10381 cagttcactt atcttcggga tgttcttcgg gtcaggtaat ctaatctttc ctgtccactt
    10441 aggtcaaatg gctggcacaa actgggtcag tgctggcgta ggatttgcca tctcaggcgc
    10501 tcttttccca ttactagcta tcttagctgt agtagtgaca aaaagtgatg gtctgtatga
    10561 cttagctaaa cctgtcggaa aatggtatgc tgccttgttc ttaatccttg ttcatttgac
    10621 gatcggacca tttttcggta caccacggac agctgctact gcttttgaaa tggctgctaa
    10681 accatttttg cctgaaaaat ataattcatt aggtatgttt ttattctctg ctcttttctt
    10741 tttacttgct tatttcctta ctgtccatcc cacacggttg gtcaaatacg tcggaaagta
    10801 tctaaactcc ttgtttcttg cattattagc aatcattttt gtcgtttctt tattccaccc
    10861 aatgggaaat ctaaatcaat ctgctgcaga tacttaccaa acaaacgctt taacaaatgg
    10921 tgtcctagaa ggttataaca cggttgatgc agtagctttg cttgccttaa gtgtcacttt
    10981 tgtccatgca gtccgtgacc ttggtttcaa agataaaaaa gtcacagaat taacagcaaa
    11041 agctggtagc ctgagcgttg cgctagaagt catgatctat tttggacttg ttcttctcgg
    11101 tgcgatgagt ttgaataaga tgtctttatc agaaaatggc ggaactgcac tttctcagat
    11161 tactaatttt tatttaggta acttcggcgc tagcttctta ggagtgatgg tcacattagg
    11221 tgtcttcact accgctatgg gactcgttgt ttcttttgca caagatttcc ataaactgtt
    11281 ccctaaagtc agttatatga catggctgcg gttaacgact ttcgtctctt ttgtcgttgc
    11341 aaatgctggt ttggacaata tcatccaatg gtctttacca gtattgatgc ttttgtaccc
    11401 attgtcgctt gcgttgatct tgctttcctt aacagcaaaa tttttccaaa agacaccatt
    11461 tgtctatcaa gtaacgatgt tgtttgctgc tgttcccgcc gtattggata tgctggcaaa
    11521 ttcgcctgct ttagtgagtc aacaaagagt tgtagcaagc atgctcgaat tttatcatca
    11581 tcacgttcct tttgctgcat taggacttgg ctggatggtc ccaacgttac ttggttacgc
    11641 tggcagctta ctcttctatt acgcctatcg cctatcaggg tacaaacaag aagcaaatga
    11701 actaccagaa gaataaccag atcaaacaca atttaacata agaaatatta aacaggaaaa
    11761 aaccagctag atgaaagaat cctattcttt cgtctagcta ttttttagat ttctttttta
    11821 cgagcacaaa aaaactgcgg acaatgattg tccgcagttt aaatgataaa attaagattg
    11881 taatagttct ttcacaatgg catttacttg gtttccatct gcttttcctt tgactttagg
    11941 catgatcgca cccatgactt tgccaaattc tttcattgat gaagcaccag tttgtgtgat
    12001 tgcttcttga acaagttgtc gaatttcttc gtcggaaagt tgttccggca tgtatttttc
    12061 gactatcaca atttcacttt ttactttttc agctaaatcg tcacgtcctg cttcttcaaa
    12121 ctcatgcaaa gaatcacgtc gctgtttcat ttcgcgagac aacaccgtta attcttcttc
    12181 ttctgtcaag tcgtgacctt ctttaatctg ctcgttttgt atagatgact tgatcattcg
    12241 gatgacttgt aaagattcct tatctttcgc tttcatcgct gttttcatgt catcgtttaa
    12301 tctacttagt agtgacacac taccaactcc aattcatcac agaactagaa ttttttgcgt
    12361 tttctagctg cttcagattt tttcttgcgt cttacacttg gtttttcgta gaattcacgt
    12421 ttacgagatt cttgtaaagt acccgctttt gacacggaac gtttgaagcg acgaagagca
    12481 tcgtcaagtg attcgttttt gcgaacgact gtttttgaca tgttaattcc ctccctccga
    12541 gcttaaaaag taaccgtaat tgttattgaa atatatctca aaaaacaaga ctgtcctgta
    12601 aacattatat ctaatgctgt tttgaccgtc aaggaaatct tgcttttttt taaaattatc
    12661 aaatgttatt ttatttaaat ccttattgac aattttcaca tcttccgaaa atctcgaatc
    12721 tatgcccttc gatcgtacat ccaccgagct gttcttcgaa ataattcatt ggacacatat
    12781 ggatttcttt cgttgttccg cacaccgtac agataaagtg atggtgatgg ccgatttccc
    12841 cttgacagca atgaaaacga aacttcatct ctccatttaa atcggtttct tccagtaaat
    12901 cgatttctgc aaaatcatgg agatttcgat aaatcgtatc gtaacttact cctttgaatt
    12961 cctgattcat atagtcaaaa acttctttag cggatacgta gcgattgcgc ttcactaaat
    13021 atgttaacaa tgcttctctt tttttagtat atttaaaacc gttatttttc aatgtttcta
    13081 gtgattgttc gatcaatgtc tgttttttca tgacttattc ctccaaatca aaatgattac
    13141 gaagtacaca ttctagagta tggtataata aacataaaaa aaatgcaagt gaggttttat
    13201 ccatgtcttc cgattctaca gaaatcgtta ctttttttgt catctccgac tctgctggag
    13261 aaactgcgac aaaattagcg caagccacta tggcccaata tcctagcgtt gagttcaatt
    13321 tattccgcag aacatttgtg acagatgaga aaacattgat gcaggcactg caagatgcac
    13381 tagaagaaaa agctcttgtc ttacacacat tgatcaatca agaattgatc gagatgacgc
    13441 gttctttttg tgaagaacat caattattta gttttgatgt gatgactccg ccgatatcag
    13501 aaatcgagcg cttaactggt atcaagccca ttcggcaacc tggcgccctt catttattga
    13561 atgagaacta tttcaaacgg attaaagcaa tggaatttgc tgtaaaatac gatgacggga
    13621 aagatcctcg tgggtttcta gaagcagatg tcgttttgct aggcgtatcc cgaacttcca
    13681 aaacgccttt gagcttgttt ttagctaaca aaaatttaaa agttgccaat cttcctttga
    13741 tcccacaagc acatattccc aaacagcttt gggagattga tcctaaaaaa atcgtgggat
    13801 tgacgaataa cccagatatt ttaaacaata tccgtaaaga acggatgcgc tcttacggat
    13861 taaatcctga tactgcttat tctgatatcg aaaaaatacg agctgaactc gattttgcaa
    13921 atgatcttta tgaaaaactg ggctgcgtag taatcaatgt cgcttcttta tctattgaag
    13981 aaacagcttc gcttattttg aatgctttag acctagaaga tcatagttat tatggaactg
    14041 aaaccaccga agaaacagac taacagtttt tcttgtcttt ttcttaaaaa aaactggact
    14101 gtgtcagcga agaagattcg ttgacacagt ccagtttgtc tctatttgca cctatcgtta
    14161 ttgatgttcc acatgtttga gtgtctggtt caaaatatcc agtacgtgag aatcatcaag
    14221 actatacaaa atcgcttttc cctcacgatg ggacttgacg acatggtttt ctcttaataa
    14281 acgcagttga tgcgatacag cagattgttc catttccact gcttgggtga ttgctgtcac
    14341 attttgttcc ccgtctttca gatacagcag aattttgaga cgagtggggt cactcaacat
    14401 tttgaatagt tggctgactt tctctacttt tgttttttgc gtgtctggca atccattcac
    14461 ctccaacttg aaaaaagact gacttctaaa gtttaccact ttttccatca gtctcaagtt
    14521 tttttattct tctgtcatct tttggataat cgcttcataa gcggctactt tttcagctgc
    14581 gtcttctgaa gagtttcctt ttacaccgat gtaaaatttg attttcggct cagttcctga
    14641 aggacggata gcgatccagc cattttcttc cagcgtatac ttcaatacat cagatggcgg
    14701 tgtcgttagt ttttctacgt ttccttcacg gtctgttctc gtcaactctt tgaagtcttc
    14761 tgtttggatg acttttgctc cggcaaattc tgttggtgct tcttcacgga atttttccat
    14821 caaagcagag attttcgcag aaccttcttg tccgctcatt gtcacagaaa tcgttttttc
    14881 tgcgtagtag ccataggttt caaaaatcgt ttgcaagccg tcatacaacg ttttgccttg
    14941 ttttttatag taagctgcta cttctgctaa taagagcaat gcttggatcg cgtctttgtc
    15001 gcgaacgaaa ggtttaacta aataaccgta actttcttcg aatccgaaca taaaagtatg
    15061 ggaatgatct tcttcaaatt gttgaatttt ttcagcaatg aatttgaatc ctgtcaatac
    15121 attgaacatg gtagccccat agtgttccgc aattgctgta ggtaattcgc tagaaacgat
    15181 cgatttcaat accgctgcgt tttcaggtaa agtgcctcgc gattggtgtg cttccaaaat
    15241 atattgagca aacagtgctc cgatttggtt tccggtcaat acttcatagg agccatttgg
    15301 caaacgaacg gctgcaccta aacggtctgc atcaggatct gttgcaatca gcaaatcagc
    15361 gccttctttc tcgccaagtc gtatcgcata ctcaaaagca gagtgttctt ctggatttgg
    15421 tgatttgact gtagtgaaat ctgggtctgc gacagcttgt tctggaacta gtacgaattg
    15481 ttcgaatcca gcttgtttca acgcacgttc gcccaacatt tttcctgttc cgtgaagtgg
    15541 tgtgtacaca agtttcaaat ctttccccat tgtttccacc aattcatgat caatcgttac
    15601 tgctttgatt tcttctagat aagcggcatc cacttcttct ccgatgatcg tgatcaatcc
    15661 gctatgttca gcttcttcgt cagataatac ttcaactttt aacggattgc tgacttcacg
    15721 gacatatttc gtcaatgcat ctgcgtctgc aggaggcatt tgtcccccat cttcgccgta
    15781 gactttatat ccgttgtaag ctgctggatt gtgggatgca gttaccatga ttcccgcaaa
    15841 cgtcttcaaa tgacggactg caaaagaaag ctctggtgtt ggacgtagac tttcgaatac
    15901 ataagatggg atattgtgat gtgctaatgt tttggctgct tccatggcaa attctggtga
    15961 ttgatggcgt gagtcataag cgatagcgac acctcggcgt tttgtttctt cgccttgtgt
    16021 atccatgaaa cgtgcaagtc cttctgttgc ttgtcttacc gtatagatgt tcattcgatt
    16081 gatcccaggc cctaagattc cccgcatgcc tgcagtaccg aattccaacg gtgcataaaa
    16141 agcatcttca agtttttcag gatcttcacc cagatcattt aattgttttt ttaagttttc
    16201 ttctaaattt tcttcatgta cccattgttc atacacttgt tcccaagaca tgaaaataac
    16261 acctctttct gaaatatggt tcttaataag tatagcattc acttattaga tataaaagta
    16321 tgaggaccaa aaaacgctta aatcaagaaa ggattctttt atcaagcacg gttgcaagta
    16381 ttcgcataaa tcggattcag tagatttcac gcgaaaaaaa agagtaaaag aacaattgaa
    16441 accgttcttc cactcttttt aaacttatgc agttgtttct tctaaagatt ccaagtattt
    16501 atacacttgt tgtccagcga tcccaccatc accaacagca gtagtgattt gtcgcaaatc
    16561 tttttcacga acatctccga tggcaaaaac gcctggtata ttcgtcttca tttcgttgtc
    16621 tgttggaatc cagccagctt cgttcgtcaa ccctgcttct ctaaatggtg cagttaatgg
    16681 atccagtcca acatagatga atactccgtc tgctgccaaa tcaaactcat tgccggtttt
    16741 gacattacgt gctttcacac ctgtcactac catttcattc ccaacgattt cgtcaacgac
    16801 tgtatcccac ataaaggaaa ttttttcatt cgcaaatgca cggtcttgga tgatcttttg
    16861 tgcacgtaat tcatctctac ggtggacgat gactacttca gaagcaaatt gtgtcaaata
    16921 gatggcttct tctactgctg agtctcctcc tccgatcact actagacgtt tgttacggaa
    16981 aaacgcacca tcacaaactg cacagtaaga aacgccacgt ccagcaaatt cttcctcgcc
    17041 aggtacgcct aatttacggt ggatacagcc tgttgcgata atgactgttt ttccttcgta
    17101 agttttatct tcacaaataa cttctttgtg gctgccgtga tccttgatat cttgcacgat
    17161 tccataagca ttttctgtcc caaatcttga aacaccttca tacatcttat aagctagatc
    17221 aggacccatg attgaatcga aaccaggata gttttcgatt tctgccgtat tgttcatttg
    17281 gccgccaggg gcgccttgtt cgatcatcaa tacggataag tttgaacgtg aagcatataa
    17341 agcagcagtc atcccagcag gacctgcgcc aataacgatt acatcataca tatgtagatt
    17401 ctcctttact taatcaagta agcatacttt acagccaatg cgaaaaaaag tctattgttc
    17461 tgcttgcgtt tctcgaatag ctttattata ctcgaaaaat ccgctgaaaa acaatagact
    17521 tttataaatc attgatcaat cattcgaaag attttctcca tttgaatcaa ttactttttt
    17581 ataccagttg aatgatttct tcttcgaacg ggctaatgtg ccatttccct cgttgtcacg
    17641 atcgacataa atgaatccgt accttttctt catctcacct gtaccagctg atactaaatc
    17701 aatacatccc caagtcgtat agcctaatag atcgactcca tctaattcta ctgcatcttt
    17761 cattgcttgg atatgtgctg ccagatagtc gattcgataa tcatcttcca cataaccatt
    17821 ctcatcgggc gtatcgactg cacctaagcc gttttctaca ataaatagtg gtttttgata
    17881 acgatcataa atatcattca tcgttattct caagcctaat ggatcaattt gccaacccca
    17941 ttcacttgct tccagatatg gatttttgac agaagcaaag atatttcctg cagtctgttc
    18001 caagagttcg ttgtcagtag tcgaaactcg agaagaataa taagaaaagg agataaaatc
    18061 aacagtatgt tctttcagca atgcggcatc cccaggttcc atttcgatct gaatgccatt
    18121 tcgttccatt tctttcaatg cataagcggg atattctcct cgtgactgga catcaatgaa
    18181 gaaataattt tctcgatctg attttctaga agcccaaaca tcttcaggat tgcaagtgta
    18241 tggataatat gctcctgctg ccagcataca gcctactttg ttctccggat ctacttcatg
    18301 agcaatcttc gttgcgatgg cacttgcaac caactcatga tgtgccgctt gatatttgat
    18361 ttgttcttca ttttctcctt cttcaaaata cagccctgcg cccatgaatg gtgcatgaag
    18421 gatcatattg atctcattga atgtcaacca atatttgaca agtcctttat agcgattgaa
    18481 gatgactcgg cacagatttt cataaaaacc aacgagttct ctggaacgcc acgcaccata
    18541 ttctttgatc aaatgcatcg gacaatcaaa atgtgtaatc gtcacaagcg gctcgatccc
    18601 gtacttttga cattctttaa agacatcctc ataaaatttc aacccagatt catttggctc
    18661 tttttcatcg cccattggaa aaatacgact ccaagcaatg gacatccgat aagtcttgaa
    18721 ccccatttct gcaaataaag caatatcttc cttataatga tgatacatat cgatcgcaat
    18781 ttttgctgga taaaaatgat tttcatcaaa ttcaaacatt ttcttttttc cagtaatgac
    18841 aggaaaacgg tcttctccga ttggcacaac gtctacgttc gctaacccac gtccgccttc
    18901 atcataaccg ccctcgcatt gattggctgc tgtagctccg ccccataaaa aatcttttct
    18961 gaatcccatc atacttcctc cattacatgt gtattgcttg ataattctct tgccagttca
    19021 tcttggactt cgattccttc tgcttttaac cggctgattg tttccttgaa ttgcggtaaa
    19081 tactgctgat gtgcaataaa caactcatcc attatccgtt ttgctgtctt tcctgatcgt
    19141 ataagcggat tgatcgtaaa cgcttgcaaa gctgttccat aatcaccagt cacggctgct
    19201 tcgatcgtca gcaattccat attcttcatt acttgcaacc agcctctttc agcagcagga
    19261 agggaaccaa acgctacatt tcgtgcgcct tgtgcgccaa cataggcagt gacttctact
    19321 acacaatctg ccggcagatc aggaacagct ccatcattct ttgtagatac cacgatctgt
    19381 gtttctttat ttccatagat cgaagcaatc gtttcacagg cagcatctga ataataagca
    19441 ccacctcgtt tttgcaattg ttcaggttta tgatccaatt ctggatcttt gtataattca
    19501 aacagctctg cttctgtttc tttcacttgc tgcgctcttg taccaatcgt ttgatattct
    19561 tctaaagcat gttccagcat ttcttcttca cggtagtaat agcggtggta gccacaagga
    19621 atcatgttca tttgttttaa ttgttctttg aaaaacggga tatcaaagat atttttcggc
    19681 aatccattgt cttcatcgta taaacgatca atcaattctg ccgtgcggtc atttccttta
    19741 ttatctgcta ctttgtgcca atggaagtga tttagaccgg caaacttgta gatcagctct
    19801 tcttttttct tgccaagcat tgttggttct gtcatcatcg ccattactgg cacattgcat
    19861 aatccgatca ctttatccca tttgccatat ctgataactg cttctgtcac catcccgctt
    19921 gggttcgcga aattgatcag ccaagcattt ggacataaac gcttcatgtc ttcaacgatt
    19981 gccaaaacca ctggaacggt acgaaaagct ttgaatatcc cgccagcacc atttgtttct
    20041 tgcccaatca tcccataata actagggatc cgctcatctt tgattcgtgc atctaattga
    20101 ccgacacgaa actgagtcgt aacaaaatcc gcatctttca atgcttcttc ccgatccaga
    20161 gttgcataga ttttgacttc atacggagaa gcatcccaca tacgctgaga catctgactg
    20221 acgatctcaa gcttttcttt tccttcttct acatctacga gccagatttc tcgtatcggt
    20281 aattcctcat atcttttgat aaatccttcc atcaactcgg gggtgtaact gctgcctcca
    20341 ccaatcgtag cgattttgat tccttttgcc atgttctcac tcctttactt tttacacttt
    20401 tattatcttt tttgtaaaca aaacaaacaa tagaaattcg tttctttcca gtaacattta
    20461 caaaagttag tgttttcttc tggatttttg taaatatctt gtcaaaaaat gtctttatta
    20521 agctatataa cataaatccg tttacaaatc attttcaaaa aagttataat ttacgtaaaa
    20581 aaacaagaag aaggtatcta catgttatta cgtgaaaaaa tcaaagaaat ctccttttct
    20641 ccttcagaaa atgaagtgat tcagtttctc ttaagcgatt ctccagcact tggcgaaatg
    20701 accatccaag agattgctga gcgatgttat gtccacccgt ccacactcat ccgtgtagca
    20761 aaaaaaatgg gttttgatgg ctgggtcagt tttcgtaatg cttttttagc tgaaacagcc
    20821 tatcttgaag ggaattttca agacgtagat gcgaatcttc ctttttccac cacagacggt
    20881 attatgacga tcgcaaataa aatggcttct ttagaacaga tgacgattca agatacactt
    20941 tctctcattc atcatgacca actgcaaaaa gccaaacaac taatgcttca agcaaaaaaa
    21001 atcgtgattt tcgccagtaa ctcgaatact ttgatttctc aagatttcat gctgaaaatg
    21061 aagcgcatca aaatggatgt cacgattgtc accacttttg gtgaatcgtt ctatgaagcg
    21121 tacaattgca cgagtgatat gtgcgggatt ctgatttctt atacgggtga aaacaatgtg
    21181 atccttcgta ccgcagaaat attgaaacaa aatcatgtgc caattttagc tttgaccagt
    21241 atcggtgatt caacgctatc ttcaatgagt acagccgctt tgcgacttac gacacgcgaa
    21301 cgactgtatt cgaaaattgg taattttacg atcaatacat cgatttgtta tcttctagac
    21361 gtgttatata gttgtgtttt tgcagaaaat tatcaacaga atttagtcca tctaatttct
    21421 gtaggacaaa aatttgataa acgccctatc agctcagcca tcatgaaaga gcctttcaat
    21481 tttgaaaata aagaagtgga ctaaaaagaa taattaagcc tagtccattc tttcacctaa
    21541 ccgttcaggt cagtgcggtg ataagtgcat cgtctggccc gaccgtctcc ccttgatttt
    21601 ccaaaatatc caaataatcc gtgtaattgg tgacgatcac aggtgtttct aaactgtatc
    21661 cttcctcttc gattttcttc ttgtcgaagc ggagcaacag atcgccttgt ttcactcttt
    21721 ctccttgctg caccaagctt ttaaaatatt ttccttctaa ctgaacagta tccaatccga
    21781 catgaatcag caattccaga cctgcttcag aaaccattcc gattgcatgt ttcgtcggga
    21841 acagggtcat gactgttccg tcaaaagggg cacgtacttc tcctttttca gggtagatca
    21901 agattcctcg acctaatgtt ccttgtgcaa aagctgcatc ctttgctgca ttcagtggag
    21961 cgatttgtcc ttgtatcggt gtttttacaa tttctttacc tgatgttttc tgtctatcat
    22021 ctgtttcatc tgcttcctcc ttccaaaaga aaaaagtcag cgcaaaggat accaccatcg
    22081 caatggcgat cccaatcaat acattaatca catctgttaa attaccatct ggtctaatat
    22141 agttgggaat tgtaaagatg cctagtccac cttgattcca agaagttaaa ttcattaacc
    22201 cactatacgc gccccctaac gcaccaccga cacaggtaaa aataaatatt tttttacgtg
    22261 gcaatgtaaa cccataaata gctggctctg taatccccac aataccagaa acaatggctg
    22321 gtattgcaat tgctttgtct tttggatttc taagcttaaa gtagagtgca gtaagtgctg
    22381 cagtttgtcc aaaagacact actgcaacag gtgtcatgat attttgccag ccattcacag
    22441 aaaattgcat caatcccatt ggtacgattg cccaatggag accaaacatc attagaattt
    22501 gccagaagaa gcctacgatt gctccataaa taactggcga aagtgctaaa agtgtttcaa
    22561 atccagccat taaaatatca gttgcgaatg tgaatgctgg tccaacgagt aaaaaaccta
    22621 agggcattgt aataagaacg gtgaagaatg gaacgaagaa attcgcgatc atctcaggta
    22681 ctaacttttt aattggtttc tgaatttggc tagctaacaa cacaataaag ataatgggaa
    22741 tgacagaaga agcgtagtta cgcgcaatcc agggaatgcc gaatactgtt tgataaatag
    22801 gtgcttcaaa aatagtgcca gaaaatatgg tcgtcagtgg tgcttctgct gcaccactca
    22861 aagcttccag cgacaagcta ggattcatta atgctgcccc aagcaacatc cctacgaatt
    22921 catttacttt aaatttacgg gcagctgtta aaccgataat aacaggcata aattggaata
    22981 cagaatcacc gatattatca aacataacaa atgtgccaga accgcggtca aatgcgccta
    23041 agaagacaag taatgctgtc agtccacgaa tcatcccacc agcagctaga atagcaagga
    23101 atggctggaa acagcccgat attccatcaa taagcatatc aaacggtctc attttttctg
    23161 ttgaaacagc ctcatcaaaa gtaaggtttc ctgctcttat aacctcttca aacacgactg
    23221 gcacatgatt cccgatgaca acttggtact gtcctcccgc tcgcattacc gtcacaacac
    23281 cgtccatatt tttcaagatc tcatcatttg ctttttcttc gttttttaat ttgaaacgaa
    23341 gccttgtgat acaatgtgtc aaaccattga tattttcctt cccgccgaca ttttctacaa
    23401 ttttgctcgc taattcttga tattttccca tttctattcc ctccaattta ttagtggaag
    23461 gtttagccaa ctgaatgtca ctacccttat ttcatgcgtt cttgttaatt tatcctcctt
    23521 ttttatttat ttacaacgat tctcatgcac taaacgcgca gtatggatcg ttaaatacat
    23581 tttttcgtcc gtggacatct ggtagttgaa tatctcggct aaaaagtctg ctattttctc
    23641 aacgcaacca aacgtatcct tatatttttt ttgaataact tggtacaatt cctcagaaac
    23701 ttctcccact tctgattttt ctccatctag gacgcgtgtc agaaaaaaac gtaggtgtgt
    23761 gacgaatcga aaaaaatgga ctgaatcttc atcaaatgta atcttatagt aatagcggac
    23821 gatatttaga atatcctgta ttgttgtagt aaacaggtag gtattgtctt gttggctttg
    23881 aatgccttgt gcattgacta aatgcaatgc gataaatccg gcttcatctt ctgataactt
    23941 gatgtggtat ttatccgaga ttttcttcac tgttttttcc ccgatggcat actctttagg
    24001 aaatagttgt ttgatatcat acagcaatac atttggtaca aatattccca ttttgtgacg
    24061 ttcgatagcc gtatacaaat gatccgtcaa tgcaatatac actgaatcat tcaactggac
    24121 ttgtagagcc tcttttgtat attcaatgat ttcacaagaa atttctaagt actcaagagg
    24181 aatcgcttgt gccaattctt ggaactgttc tgaaagtccg tcatttgtca actgaaattt
    24241 tttgtcgatg catgagtcaa aaatcgggtc acccactttc tttttgaatg ccaaaccttt
    24301 tcccatcacg atcatttcct gatttctttc atctctggtt attacgacgt tgttattcag
    24361 tactttctca atcagcatgt tttactcctt tccatgatat atagggctgt atccaaaaca
    24421 aaaagaaaaa cccaaaaagc cgtccatgga accatgggag actcatcagg tttagcttgc
    24481 atcaggcaat cactatccaa gttgtatcgt tttctttata ttatcgaaag caagcgcttt
    24541 tgtcaacgct ttcttagtgg ctattaggca cttctgacta gctgtcgaat cttttttaaa
    24601 tctggtgact acgacactac cgctcacaga tgaagaatct gggaagctct gttttatttt
    24661 cactctagcg cttaggtcaa aatgctacta tgctactgtc ccaacttcta atatacggtg
    24721 ttccagtatc agcttatcgg tgataagcca aatttatgca aaaatctgga aagccttttt
    24781 tacaattttt ttcttatcac ctaaagctga acggagccct gtcacaacca ctttttacac
    24841 accactgctt tgcagtttgt acatatctgc atacaaacca ttttgcgcta gaagtgtttc
    24901 atgattcccg cgttctacta tatgaccttt gtctaaaact aaaatcaatt ctgcatcccg
    24961 aatcgtggac agccgatggg caatcgcaat cgttgtccgt ccttgtcgca tttttgtcaa
    25021 tccttcttgg atcaatcctt ctgtttcagt atcgatattt gctgtcgctt catccaatac
    25081 tagtatttta ggatctgtca cgattgttcg cgcaaaagtg atcagctgtc gttgtccgct
    25141 agaatagctg gcaccgtgtt cgatcacttt tgagtgatat ccttttggta attcatcgat
    25201 gaacttgttt gcttgaacaa actcagctgc atggcgaatc tgttcgtctg tgatagttgg
    25261 attcatcatc cggatatttc ctgcgatatc tccataaaac aagaaggcat cttgcaagac
    25321 caatcccatt ttcttacgta actcttccat tggatattct cgaatgtcat gatcatcaat
    25381 caagatctgg ccttcataaa attcgtaaaa ccgcatcaat acgttgatga tcgagctttt
    25441 tccgcttccg gtgtgcccca ctaatgcaac ggtctctccc ggattagcga caaaagagat
    25501 attgttcaat acattgtgct ctccatcata agaaaagctt acattgcgaa actcgatctt
    25561 acctgaagta atcgtttgat ctgctccagg attttgttgc ggtgcatatt cttctgtatc
    25621 catgattttc aaaatacgac taccggcaac cattccatct gtaaaggtgc tcaaaaagtc
    25681 catcatttga gacattggat taaaaaatcc ctggacatag gtcgtaaatg catagatcag
    25741 tccaacttct acgggagaat gcagcgcatc gatcccaaac aaagtcaacg ccaacgcaat
    25801 cgccaatgca tatagaaaat tgatgatcgg acttaacaga atcgaattcg ttttgatcat
    25861 cgcatagcgc gtgtctaaat actcgttgtt cacttgttca aattctttag ctaaacgagc
    25921 ttcttggcga aactgctgga taatacgcat gccggaaatc gattcattca atttcgtgtt
    25981 cagctgactt aatttttccc gcatattccg gtaaatacga gaactgaatt tctggtaata
    26041 ccagatgacg attcctaaaa tcggcaagaa caccaaagtc cacaaggcaa tttctccatt
    26101 gatttgaaac atggcaatga aggaagaaac gactgcaaag atcccagtca ataccatcaa
    26161 aaagacatac caaaattcaa atagcgtttc tgtgtcattt gtcactcgag aaacggtcga
    26221 tcctgccggt gtctgatcaa aataacgcat acctagtgta tgcagctttt caaataatct
    26281 tacccgaaca tactgatagg tcttcaatga agccatggaa tacaaatacc attggaaaaa
    26341 ccaaatgata cttttgacta tgactccaaa caagtacata ccagcaaaaa acaaaataac
    26401 ttgtgtcgta gccgactgtt tagccagatg atcatccata aaagtttgga tgatccttgg
    26461 caataggaca ttgatcacag acagagcaaa cgcaaataag atagcaacga tgaacgttct
    26521 tctaaaaggt ttggcgaact tcatcagtcg tttgatgatc ttcttctgtt ctttaagtgg
    26581 aatcgatttt gtccattccg attgataatt ttcatccatc aggcctcgcc tccttctatc
    26641 ttcgcttcta attgctgttt ctcccacatt cgttcatacc agccgcctaa tgcaagcagc
    26701 tcatgatggg ttcctcgttc aatgattttc ccttgatcga caacaatgat ttcttttgca
    26761 tgcatcacgc tgcttaaacg gtgagctgca atgatggttg ttttctcttt tcttatttct
    26821 tttaaattat gcaagatagc ttcttctgtt ttggcatcta ctgcagaaag tgcatcatct
    26881 aagatcagca attcaggatt tttgatcaat gctcgagcaa tcgaaatcct ttgtttctgt
    26941 ccgccagata aagagactcc acgttctcca actaaggtat cataaccttt tggcaattcc
    27001 ttgatttcat tgttgataaa tgctaaagaa gccgcatgct ctacttcttc ttctgaaagt
    27061 tttggattag aaaaacggat attatctcga atggtcatcg aaaacaagaa gtgatcttga
    27121 ggcacatagc cgattgatcc taataaagca ttcaatgaat attctttgat cgaacgtccg
    27181 ccaaattgga tttttccttg ataatcatcg tattcacgca tcagtaattt gatgatcgtc
    27241 gtttttcccg caccagtttt cccaacaata cctaatgttt ctccttcttg gatcacaaat
    27301 ttgatttgtt ctaatgctgg tgattcttct ttcggatagt taaaagagga aatagctaca
    27361 ttcagctcgc cttttgccgg ggtctgcact gcacccagtt gttccacgat atgcgtcttt
    27421 tcatgcaaaa gttcattgac gcgatcataa gaagcatttc ctctttccaa tacgttgaac
    27481 aatcgaccaa tcgcaaacat cggccaaaca agcataccga tatagctaat aaatgagacc
    27541 atctgcccga tcgagatcgt gccttccaag acatactttc cacctaaaat aattgttgca
    27601 acataagaca gaccgataat caaggtaatg aatggatcaa ataaggagtc taaaaaattg
    27661 actcgtttgt ttttaataat tgcatcttca atcttttctt caaaatccgc gagatcttct
    27721 ttttcttgtc caaatgtttt aatgactttg attccagtaa tactttcttg tgtcttatca
    27781 ttgatactag aaaaagcttc ttgcgaatca cgaaaagcat cgtggagttt tgaccccagc
    27841 actcgtgaag taacggctag taaaggcaga ggaatcagcg caatcagtgt taagcgccaa
    27901 tcaataaaca aaatcatcgc aatgatcgtt gttccgcccg tgatcactga atctgcaaag
    27961 gtcaagatac cagctcctgc tacattttgg attgcattca aatcattcgt tgcatgagcc
    28021 atcaaatctc cagtccgata tttttggtaa aagagattgt ccatttctgt aaaatgtttg
    28081 aatagctgct gccgaagttc tttctccaac cgagctgcac ttccccaaat attcgtccgc
    28141 cagatataac gaaaaagata ttgtaagaga gctgcaagga tcaataataa gatccaccag
    28201 gaaatgatct ttattgataa atttttatcg gcaatctcat caatgatgat cccaatcacc
    28261 tttggaggaa ctaactgtac caaggccact gcgaataggg agaaaatccc aattaaataa
    28321 tgtttctttt cctgtcgaaa aaaccaacct aattttctaa aaatcgacat tttactcctc
    28381 ctttgtcttt atcaagagca taggattgtc tgtgcttgtc ttttttgctg aatagtctga
    28441 atagtctaac aaattacgaa ataaaaaacg gactgaaaaa atcagcccgc aaaaagtctt
    28501 tgggtcagaa cctatttttt cccttggttc ttcatttgct gcatcatctg gcgaattttc
    28561 ttctcagatg gtttttgccc cattgacatc atcattgatc gcaacatgtc ctcattaacg
    28621 ggaggatttt tttctaaata gtctttcata tatttacgcg ctaggaaaaa gccacctact
    28681 gcgccgatta gcaaagcaat aatagcaata agtacgacaa tacctgttga taccatataa
    28741 atctctcctt tctatttccg aattgctctc aatgatttat tttactaaag tttattgaaa
    28801 aaagaaaggc gtattcttta gtttcatgca aaaaatagtg gtttttttag agaagaacaa
    28861 aatttctttc attaaccagc aagacacgcc acgaaaaaaa attaggcata ccgaatattt
    28921 ttctttaatt ttttctttct gaggtgtaga atgtaatcgt agataaatag gaaaggtggt
    28981 tttttcttga ataatcaatc caacaaacaa tttcataagc atcgacttat tgaacataca
    29041 aatggtttat ctttagctga agtaaatgga acagtggatg tacccaaggg aaaaagtttt
    29101 tttaaaacac ttcttgctta ctctggacca ggtgcattag tagcagtggg ttatatggat
    29161 ccaggtaact ggtccacttc gatcactggt ggacaaaatt tccaatattt attgatgtca
    29221 gttattttga tgtctagtct gattgctatg cttttacaat atatggcagc aaaattagga
    29281 atcgtgaccc aaatggattt ggcccaagca atccgagcac ggacaagtaa gacattgggt
    29341 gtcattttat ggatcttgac tgaattagcc attatggcaa cagacatcgc agaagtcatt
    29401 ggtgcagcaa tcgccctcta cctgcttttt gatattccat tagtgattgc cgtatttatc
    29461 actgtgttag atgttttcgt cctcttattg ctgacaaaag tcgggtttcg taaaatcgaa
    29521 gctttggttg tctgcttgat tttcgtcatt ttattcgttt tcgtctatca agttggcttg
    29581 tctaaccctg attggcacag tttgtttgcc ggctttatcc ctaacagtca tacattctca
    29641 gactcaccgg tcatcggcgg tgaaactccg ctgactggag ctttagggat tattggtgca
    29701 acagtcatgc cgcataactt atatcttcat tcagcagttt ctcaaactcg gaagatcgat
    29761 caccaagatg aagaagatgt agcacaagcg gtacgctttt ctacttggga ttctaatatc
    29821 cagttgtcaa tggcttttct agtcaatgca ctgctattga tcatgggtgt cgctgtattc
    29881 aaaaccggtt ctgtccaaga tccttccttt tttggtctgt atgaagcttt atctgatcca
    29941 tcagctatga gcaatggtat cttgattgct gtcgcaaaat ctggtatctt gtccacttta
    30001 tttgctgtag cacttcttgc ttctggccag aactctacta ttactggtac gttgaccggt
    30061 caagtcatca tggaaggctt catccatatg cgtatcccga tttggctaag aagactagtc
    30121 acacgattgc tttctgtgat tcctgtattg atttgtgttt tgtttacaag tacaaaagga
    30181 acgatcgaag aacataccgc tttgaataac ttgatgaatg aatcccaagt ttttctggct
    30241 tttgcattgc cattctcgat gattccttta ctgatgatga ccaatagcca agcggagatg
    30301 gggagccgat tcaaaaattc atttgttgta aaactacttg gctggttctc tgtgattagt
    30361 ctgacttact tgaacttacg aggattacca ggtcaaatcg aaagtttctt cggcgagcaa
    30421 gccacctcag ctcaactagc attggctgat caaatcgctt atgtaatcat tgttcttgtc
    30481 cttctcctgt tggcatggac gattggtgat ttatataaag gaaacaaaaa atatgaacag
    30541 cgtttgcaag aaacgataga agaaatcgaa tagaaactga ttcttttaca caaaggaggt
    30601 tgtgacagaa gtgggcagct tcaagaaata agaaggaatt ta
//
LOCUS       DACEPD010000027        30050 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.027, whole genome shotgun sequence.
ACCESSION   DACEPD010000027 DACEPD010000000
VERSION     DACEPD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.027"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(348..1292)
                     /locus_tag="IW797_001157"
     CDS             complement(348..1292)
                     /locus_tag="IW797_001157"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1223944.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1588..2532)
                     /locus_tag="IW797_001158"
     CDS             complement(1588..2532)
                     /locus_tag="IW797_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1223945.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2753..3967
                     /locus_tag="IW797_001159"
     CDS             2753..3967
                     /locus_tag="IW797_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1223946.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3989..6004
                     /locus_tag="IW797_001160"
     CDS             3989..6004
                     /locus_tag="IW797_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAQ1223947.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6087..7460)
                     /locus_tag="IW797_001161"
     CDS             complement(6087..7460)
                     /locus_tag="IW797_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1223948.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7486..7770)
                     /locus_tag="IW797_001162"
     CDS             complement(7486..7770)
                     /locus_tag="IW797_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1223949.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7772..9787)
                     /locus_tag="IW797_001163"
     CDS             complement(7772..9787)
                     /locus_tag="IW797_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1223950.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9966..10271)
                     /locus_tag="IW797_001164"
     CDS             complement(9966..10271)
                     /locus_tag="IW797_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ1223951.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10360..11391)
                     /locus_tag="IW797_001165"
     CDS             complement(10360..11391)
                     /locus_tag="IW797_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ1223952.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11598..12473
                     /locus_tag="IW797_001166"
     CDS             11598..12473
                     /locus_tag="IW797_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1223953.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12625..13467)
                     /locus_tag="IW797_001167"
     CDS             complement(12625..13467)
                     /locus_tag="IW797_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1223954.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13559..15436)
                     /locus_tag="IW797_001168"
     CDS             complement(13559..15436)
                     /locus_tag="IW797_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1223955.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15433..16854)
                     /locus_tag="IW797_001169"
     CDS             complement(15433..16854)
                     /locus_tag="IW797_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1223956.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16857..20246)
                     /locus_tag="IW797_001170"
     CDS             complement(16857..20246)
                     /locus_tag="IW797_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1223957.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20503..21894
                     /locus_tag="IW797_001171"
     CDS             20503..21894
                     /locus_tag="IW797_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1223958.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21967..22614)
                     /locus_tag="IW797_001172"
     CDS             complement(21967..22614)
                     /locus_tag="IW797_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1223959.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22622..23308)
                     /locus_tag="IW797_001173"
     CDS             complement(22622..23308)
                     /locus_tag="IW797_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1223960.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23314..24387)
                     /locus_tag="IW797_001174"
     CDS             complement(23314..24387)
                     /locus_tag="IW797_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ1223961.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24609..25283
                     /locus_tag="IW797_001175"
     CDS             24609..25283
                     /locus_tag="IW797_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1223962.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25288..26628
                     /locus_tag="IW797_001176"
     CDS             25288..26628
                     /locus_tag="IW797_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1223963.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26777..27763)
                     /locus_tag="IW797_001177"
     CDS             complement(26777..27763)
                     /locus_tag="IW797_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ1223964.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28487..29518
                     /locus_tag="IW797_001178"
     CDS             28487..29518
                     /locus_tag="IW797_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1223965.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29627..>30050
                     /locus_tag="IW797_001179"
     CDS             29627..>30050
                     /locus_tag="IW797_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ1223966.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT         8908 a         5624 c         5399 g        10119 t
ORIGIN      
        1 tataaaaaaa ttttgcagtt tttcttactt tttttgtcgt ttaattgaat gataaaaacc
       61 agtttcttta ttcagttaat caaatatgaa cgaattagat atgaatcatt gtatttgccg
      121 ttgcggaagg attttttaat taaccacaga taaacattga taacgattcg cttattgtaa
      181 tcaacagatt aaatttcata tgttcatatg tacggataca ttttcggacc gattaatttt
      241 aaccattctg tttatttgct tacctattag atatcaataa aaaactgctt aacagctatt
      301 tcagctgtta agcagtttta ggatttttag ttcatattca ttgtttctta taatacagtg
      361 ttcaatactt ctcttaaagc acttgttgat ttgctgaaca ttgctttttc ttcttcgttg
      421 attgataatt caacaacttc acgaacacca ttttcgtcaa cgattgatgg cacacctgta
      481 aagatatctt tttcgtcgta ttcaccagtt aaataagcag atacaggcaa tacagcttgt
      541 tcgttattca agatagcttt tacgatacgt gcagtactca taccaatacc atagtaagtt
      601 gcttttttac gatcaatgat ctcataagca gcattacgga ctttatcagc aatcacatct
      661 aattcatctt gggcaatacg atggtctttt tctacaattt caaacactgg tttaccaccg
      721 acagttgtat gtgaccatgc agcaacttct gaatcaccat gttcacctaa gatatagccg
      781 tggacgctgc gtgggtcaac ttttaatttc aatgcgattt ctttacggaa acgagttgta
      841 tccaatgttg ttccagtacc gataacacgt gatgttggca gaccagattc gttccaagcg
      901 atatatgtca aaatatctac tgggtttgaa gcaacaacga taataccgtc gaatcctgat
      961 cccatgattt cttttacgat ttggcgcatg attgacgcat ttgttttaac taaatctaaa
     1021 cgagattgac caggtttttg gttgatacca gctgtgataa caacgatatc agcatcttta
     1081 cattcttcat agccacctga ccatacagcc acattttcat cgccccaagc cataccatct
     1141 aaaaggtcca atgcttcccc ttctgctttt tcttgattca catcgatcaa tactaattca
     1201 ttagagattc cttggttgat cattgcataa gcgatacttg ttccaacaaa acctgtaccg
     1261 acgattacta ctttacgact tgtttttttc atgaataaac agctcctttt cataactatt
     1321 tgttactttc atgaaaagta tagtcttgtt tattcatttt atcaacatgt taattataat
     1381 agttgttatt ataatttttg atggaaacga attcctgaat acgcataaca gataaatgac
     1441 aaatgtttac acctatatcc cctgataata caaccaaaag tttagaactg aaaataaatc
     1501 caaaaaaatt cgctgcaacg atataataag acagcaatcg cacctaaaac aattttcctt
     1561 catatgcttt agtgaaagat tttcttctta atgtttattt ctccattcat tgggagtaca
     1621 tgttgaatac ttcttgaata atttgaataa agaagtcgtt tgtttatatc cgatacgatt
     1681 agcgatttct tccaacgaaa aactcgtttc ttgaatcagt tccaacgcaa agtcgaattt
     1741 tttctttaga accagttctt gaaaggaagc atcaaaatac attttcaatt tattactaag
     1801 atagaccgga ttgtagccaa aatgagctgc tgtttctttt aacgtacaag aagtaaaatg
     1861 cttatcgata taatctaata ctctaagcat ctcattttct gcgcatccat ctgctgatga
     1921 aaagctttgt tgttctaata aatgagtcaa ttcgatcagt aatagtgatg tatagagatt
     1981 taacgaacgg atcttatgac tgtatgtatc aaaataaatc aacagcatat tttcaatcaa
     2041 atgcgtcaaa cgacttgttt ttccgccgcg aaaatgaatg aattgtgtat gttccccttt
     2101 ttcttttgat gcatttatca aaaaagaaga aagtagatta tttgtgcttg ccaaatgatt
     2161 caatatctct gtagataagg tttcttcttt taataagatg ttgattaaaa tatcctcttg
     2221 ccctgtgtat tcgattcttt gagtagcttc tttatcaatg atcaccacgt cactttcttc
     2281 catctctatc ctttctccat taataatttg taaacatgta ccgctataca tataattgat
     2341 ttcaacaaaa tcatgtgtat gctcgggaac ttccgcatat cgacggtgtt tactgatatg
     2401 aatatttccc tgttgaaata gatcccctcg gtggattttt ggcggtgtct gaagatggta
     2461 agctaagttt aaatcattga tattcattcc ttgttcacgc tgcagagttt cgattgattc
     2521 ttcgttacgc aaaaaaaatg tctaattcag ctagttccaa ttgactccct cctttttgta
     2581 agcggtttct ttcgcttatt agttcacgct gtactgctat tatatgcaaa gctatttttg
     2641 gtttcataaa gactttctat ggttgggaca tgaacttcct gtgattccgc tattgagaga
     2701 ataaccactt catactatat ttttgataag cgcttacaaa aatgaaagga acatgcttat
     2761 gaataaaaag atgatgaaat tttctctgct gtcagtctct cttattttga cttctgctgg
     2821 tgcagtcacg tctgcaattc ccagtatgtc agctacttat gccacgcgtt ctttatctgc
     2881 tgttgaatta ttaacgactg ctccctcttt tatggtcact atttttgttt tgattagtag
     2941 ttttattgct aggaaaatcg gacaaaaaca aactgtgatc atcggattag tgattgcatc
     3001 gatttgtggt atcctccccc tcttctcaca aagttacagt attgtcttag cttcaagagc
     3061 tggactaggt attggctttg gattgatcaa ttctttggca gtcagcatga ttgctgcctt
     3121 ttatcacgga gatgaacgag cctctatgat tggttttcaa agtgcatttc aaggatttgg
     3181 tgcagcttta atgacatttg ttgcaggaca gctagtacag ttcggttggc aatatacctt
     3241 tttagtctac gccatcagct tacctattct tgtcttgttt gttttgtttg ttcctacacc
     3301 tgaaacaact gaacttcaat cgcatggcac agccccaaca agacaagtaa tcaaaacgaa
     3361 tcctaaaatg attagttatg gtatttttct cttgctggtc atcgtggtat ataatgctgt
     3421 atcgattaaa ttagccacag tcctgacgac ttattctcta ggaaacgaaa ctaatgcagc
     3481 gaatatctta acaatcatgc aattggcaag tatggctgcc ggagtttgtt ttggctggct
     3541 tcagacaaaa gcacatcgct atatgcttac actttcttta ctactgatgg gaatcggctt
     3601 tgtattgatt gccactgtga taaacctgta tgtgattggg tttggtgcga tcctaacagg
     3661 aatttctttt tcgttattca ttccttactt attcaaccaa gtaagtatcc aagcgcccaa
     3721 gtacgcggag agtttcaata cttcaatctt attggttggc gctaatttag gtactttcat
     3781 tgcaccatat ggtttgctct ttctctcttt tgcttctctt gggagtaaaa cacttgctgt
     3841 tttcgtcaat ggcagtatct tgctgttgat tagcgcgctt attagccttc tagtagtaca
     3901 caggacgaat agaaagacag tgataagagt agatgaagaa ataaaagaac aattgacaac
     3961 aaaataaatt tgatggaggt cgtgtaaaat gacaaatgaa aaacataaag atagatggct
     4021 ttggtatcct ggagactttg agatccgcca tggactgcta caaaatttcc aacgggaaga
     4081 acgaggtttt gactggccag cgtactggta catggatgat tgccatcgga acgtcaagtt
     4141 taagagatat tactttcttg atcaaccttc catgtttaaa gtgacgatcc aaggagttgg
     4201 gtatgtagaa attaatggac aaaaacatcc atgtggaaaa tggctgactt gtccagctgg
     4261 taaagcaaaa attcgtatct ttgtaggaca tacatcaggg ctaccagcga tgtttatcga
     4321 gggagacgaa gtaaaatcag atattggctg gacagcaagt aattttattg aagaatatcc
     4381 agctggctgg agtccgcttt atgtcgatat cgcaaaagat ccaaatcaaa tctattatca
     4441 aaaagaatgc gtccatccag tcactgaaac ggagattggc ggaggaatct tattcgattt
     4501 tgggcgagca gtgaatggct gtgtagaact aatatctctg gatgaccata tcgaatctat
     4561 cactctatgt tatggagagt ccgatgtaga agctatggat atcgattatt gctactataa
     4621 gcaaatagga atgacaagaa aaatgaaagc tcgtaaacgc gcttttcgct atttgtttat
     4681 accagaggtt tctactggaa tggttcaact tcaagttttt catgaatttt tatctctttc
     4741 gaaacaagct gctttttcaa gcgattcttc tttactgaat cagatctggt cagtatcaga
     4801 agaagccttc aagttatgta gtgatttgtt ttttatcgac ggtatcaaac gagatcgctg
     4861 gatttggtct ggagatgcgt atcaatcatt catgatcaat caatatcttt tttttgacga
     4921 ggaaatcaat aaacgaacga tattagcatt aagaggacaa aatgaaatca agcagcatat
     4981 gaatactatc gttgactatt cgatcttgtg gttgattggt atcgaaaacc actatatgat
     5041 gacgaaagac tatgaatttt tacgcatcgt ctaccctaag atgcaatcat taatgagata
     5101 cttgatggag caaacgaatg aattagggtt tatttatggg agagcaaaag attggatatt
     5161 tattgattgg tctgaaatgg ataaagaagg aacgcttgct gcagaacaag tcttattact
     5221 gaaagcctat cattccatga tcacttgcgg agatatctta gctgaaacat atggagagaa
     5281 cgaaaattat catactcatc tctatcaaga aaaatatcaa ctattgaaac aaaacctatt
     5341 tgactacttt tgggatgaag aacttggcgc ttttatcgat tgctatgaat ctgggagaag
     5401 aaatgtgacg cgtcatgcga atattttcgc tgttttattc gattttgcta acaaagacca
     5461 acagcaatcg atcttaaaga atgtcttatt gaatgacact atcacacaga ttactactcc
     5521 atatttcaaa ttctatgaac aagatgcttt atgcaaactc ggacagacga atatcgtcta
     5581 ccaaacgatt cttgattatt ggggagggat gttatctcat gatgcggtaa ctttctggga
     5641 agaatacgac cctactcaaa caggaaatga aaagtacagt atgtatggag atccttatgg
     5701 aaaaagctta tgccatgcat ggggagcaag cccaatctat cttttaggac gctattttat
     5761 cggtttgtat ccaacaaagc caggttatga agaatttatg atacaaccta atctagattc
     5821 atttgaaaca ctgaattgta cgctacctat caaagatgga aagatttcgc ttgaaaaaac
     5881 aaaagataag atcgtgatta cttctgatag aacaggcgga acagtgaaaa tgaatggtaa
     5941 aagttatcct cttctagcaa aacagccact tgtcttaaca agctattcaa aaataaatgc
     6001 ataaaacaaa aaactgcttg cgacatatca gaagacttta aatcttcttc tgatattgtc
     6061 caagcaattt ttattttgaa gtttgcttaa ttctgttctt tagaaacaat gtttgttctt
     6121 tttccggcaa aaagaaagct agataatacg acaagtaaaa agaaagcgat gattccaact
     6181 gtcaaggcta tttgaccgta ttctcctagt ttcccaagta caagtcctgg tactagataa
     6241 tcagtatcgg caaatgtaga agaagcgatt ttaagatctc ctaatactgg aaggataaat
     6301 aatggcagcc aactaatcaa taagctattg aaagcagaac ctaataccgc ccctcttcta
     6361 ccgccagtcg agttgccgaa gacaccagaa gcagctccgc agaagaaatg tcctactacc
     6421 ccaggaatga taactgtagt ttgcaagcct atcatagcaa acatggatag tgttcctaca
     6481 aagaagctaa caaaaaagcc aattaataca gcattaggtg catatggaaa aacgatagga
     6541 acatctaatg ctggtttaga atcgggtacg agttttttag caatcccttg gaatgctgga
     6601 acgatttctg ccaacaccat tcgaacccct tgaaggacga taacaaaccc agctgtgaat
     6661 gttccagctt ggatcagtgc aaagattaca gcgttgtctc cattgcttag ttctctttca
     6721 acaaatccag gaccagctaa caaagcaagt gcgacatata cgatcgacat caagagcatg
     6781 atcgatacag tagaatctcg caaaaagctg aaacttttag gaatctttat gtcctctgta
     6841 gaacggtctt tgtttccaaa aaactttccg atccatccac tagaagcata accgatattt
     6901 ccggtatgcc ctaacgcaac ttgattgttt ccagtgatgt tcttcataaa cggttgacaa
     6961 agagcaggcg taatagttaa tagtgttcct tcgaagaccc cgcctaataa gatcatccac
     7021 aaatttgcag taaaccccgc gcttactaaa atgactgctg ttagacaaga aacataaagc
     7081 attgcttgac ctgtaagaaa aatgaattta tatcttgtga atctagcaag taaaatattc
     7141 acgatcatcc ctactagcat gatcaaagca gtaggtgtac cgtattgttg caaagcaatc
     7201 gccactactg cttcattgct aggaactact ccttgagtat gcagagctgt ttggaacatc
     7261 gaagcaaaag gggttagtga attactgatt aaaccagcac cgccgatcag tactaggaat
     7321 ccgacaaatg ttttgatagt tccttggatc acttctgaaa atttcttctt ctgaagaacg
     7381 agaccaagca gtgcaatcaa agaaacgagg atggagggct gactgacgat tgacacaaac
     7441 aaattcaaca atttatccat tgatagttcc tcccttgaat catttttatt cttttgacaa
     7501 gatcttcgtt actttttctt ttacttcttc gctgtcaatg atcgattgca aaggtacgag
     7561 cttttcatca ggtatagaag ttagcgtatc tttcaaagaa tgctcaatga agaaataatc
     7621 agccatatcg ctagatacac ttccaacatc cgtatgactt acttcaaact cgtccacatc
     7681 tgctccaaga tctgcaagtg cttgtttgat gttcatctct accataaaac ttgttcctaa
     7741 tccactttga catactgcaa aaattttcat atcattcgtc tccttttctt atgatcgcta
     7801 atatttcttc ttttgttgat gcgtgtaata aatcttgaag attttgttta ttagaaagta
     7861 gctttgttaa atttgccaaa gcccgaagat gtgcctcatt atcgatagca gctaaacaga
     7921 tgaacaaact aatgggatgg gctggattat caagcagatc cacaggttgt tgaagtttca
     7981 gcaaagacat ccctacttcg tttacgccat cttctggacg agcatgaggt aatgcgatgt
     8041 gatcgccaat atggataaac gcaccatact gctccacgcg ctcgatcata gctttgatat
     8101 aactttcttc tattttattt tctgataata gcggctgtgc ggcaaatgtg attgcttctt
     8161 gccagttttt tggttgatcc gataattgga tcatttcttc tgttaataat tcagatagca
     8221 taggacgttg atcctccttc ttttctaaaa gtttgttcat tttacgattt aatacttgat
     8281 agagcttttc ttcgtctacg ccttctttta attcgatata aggcagtagc gccgacaaca
     8341 attcttgggc agaaggcatt gaaaatcctg gtatcagcca atctctttgt acttgattga
     8401 tcagctgatt tttttccaag tcagtcatga ttggatgcag aacatagaga gactttttct
     8461 tatcagtaag tgtgagaggt acagtagaaa aaataatatc gtaagaagtt tcgtcaacct
     8521 cgtgtaagcg gtcagctgaa ttagtctctg taaaaaagat catcgggaat aattgctgaa
     8581 gttcactttt taagatcaat gaagagctaa tgccacttgg acagacgatc acggctctta
     8641 tttttctatt tctttcttct gcgtccgctt ttctgatctc tccgccaaat agtatagtga
     8701 aaaaaccaat ctcttcttca gggatgctct tccctattcg tctttctaac ggaagtaatg
     8761 cttttcttgt catttcgtag atggaagcat attgatgctt aatttgatcg atcatcacat
     8821 taggtaaata aaaagaatag gaaatacgaa aataggcagg gaccaagtga taaaagacat
     8881 tcatcaataa ttcacgaaac gaagcaaatt ctacggccat caggaattcc atgcgatgga
     8941 tgacttctga agaacattcc agcaaaaaat caagtgcagg ctcctggaca tctccttcaa
     9001 cgatcgatgc taagcaaaga gagaaaaata taatctccga attttgatgt tcggaatagc
     9061 caaggccagt caatacttgt cgactaatgt cagtgactgc aagagaagcc aacagttcag
     9121 catcttttac cagttccatg gatgtttttc caattcgaat ggttgtcaaa ctcaaataat
     9181 aagctaattc atccattcga tttggtgcga atcgaagatc tgatagtgaa atcgttttat
     9241 ctagcaaatc tctaactttg gcgtaataat caatggaaag ggttgcggtt aaatacgtga
     9301 gcccaaatct tccagcaagt gactgcatca aacgagcagt gcgatttttt gcttctcttc
     9361 ttaatattgc ttctgaccct agcagcctaa aaccagtttt cctagtatac gacagctgac
     9421 aatcttccac ttctaattca tttcttatct ttttgatatc tgacaggact gtccctttag
     9481 aaacctgaag aaaatcttga aaatgaaaaa cagaaacaga cgttgcagtc gaataaacca
     9541 tcaaataaag aagatctctt cgctcttgtt cggtatacac agcttcaaat cgagtaatat
     9601 ccaaaaattt ttctgcccag tcgattgctc ggaatttctt tatacggata cgggtatcat
     9661 ccacatattc ggcaaaagga gttaattgtt tgatcgcctc gcttgtattt acttcgatga
     9721 tctctgctgc tttttctaga ctgatttctc ttggtgtttt caggagataa ctaattttgt
     9781 agttcacagc gctgttcacc tcctacttat attctagtat ggtagcgctt ttaaatcgag
     9841 gggtgtttgg tacaacttac agttggattt tttttacgac tgtaccgaac gatttctatc
     9901 gaatggatac agaaaaaaag caggtgacca aatgtctcct gcttttatga attgatttcg
     9961 tccgatcaat gatgatcatg tttatttttt gaggtcagtt cttcaaattt ttctttgaca
    10021 tcttcaaccg ctttttcgat atctttttca aagtgattgg gctcattagg ctttgtttcc
    10081 tcttctacag aatttgaacc agataaagct gtttctttat tttctgtttc accagagttt
    10141 gtgccagcta atgattcttc tttgtcttct gtttcaccag aattagttcc tgctaaagcc
    10201 acttcttctt ctgttttagg ggccattgaa cctcttaatc gtttgtgttt ttcaccgtca
    10261 taatttgtca tgttggttcc tcctagtagt tctatacttt ttcatcgtac aataagacag
    10321 tttatgcgtc aaaaaacaaa cccgattctt acaaatatgt catatttttc cttctgattc
    10381 ttgaatgctt ttgattcctt cttccaaaat tttcatttgt aagattgttt cttcatcttt
    10441 gatgactttt tctgcgccat taactaatgt ctcgtaaatc ccttcgtaga cacggctgta
    10501 atcaccaacc tcggaaatta ctttttcttc atgataactc ccttgttcat ctatataggt
    10561 caatacacca taatgttcgg gcaaatcgat accaaagtct tcatgatcag gcatgtaaaa
    10621 cattttcaga tgttcttctt gtcgatcttt tgtttgtttc acgaacatcc ctttttttcc
    10681 gtaaacgacg aaacttggac gttctttgat gcggaaataa ctggatttta tcgaaatttt
    10741 catatttcca tagaaaaaat ccagatcgaa gtaatcattc attcgtcctt tccctaaaag
    10801 ttgacgtaca tcatagctga tatcatctgg ttcgccgaaa taagaaagaa cttgatctac
    10861 tgtatgacaa gcatgtccat ataaatagct gttcgcagta gaaaattcat gcgtattttc
    10921 tggtacttcc ggtctgaaat aatcataatg catttctact tctaaaatat cccctaattt
    10981 tccgctttcg attacttttt ggactgtcaa gaagtcagaa tcgaaccgtc tgttttggta
    11041 acattggata aatagacctt tttcttttgc taatgcaaat aactcttctg cttcctttga
    11101 cgtttctgca aaaggtttct ctactagtac attcttacca tgttcgagtg ccatttttcc
    11161 atattcataa tgaaattgac taggtgtagt gatgacaact aattggatct caggatcatc
    11221 ccaaagatca gacagctgat cagtatattc gatgtcttcg aatgttttcc acactggttc
    11281 agatggggaa aaaatttttt tgatattgat tttgttcgta agttttcttg aaaaaggcaa
    11341 atgatagcga tttgtactct ttccattgcc aagataagcg attgtaagca tgagtggtgc
    11401 ctccttttat tgatgttttc agtatatcct agaaaagcgc agaaagtatt tataatagct
    11461 attctggtaa aaaagttcag aaaaacactt gttatcattc ggtttggtga aattaatgtt
    11521 caaagtaagg ataaaaatca aaaagatagt agcggaacca agtgtttatc agtaagatag
    11581 aatttggagg tcagatcatg cttattgaag aaaaactaac aaaacaagag ttgtttacca
    11641 cgacagagaa acggattgtc gattacatcc gtaggaatat cgaagctgca gtctacatga
    11701 cgattgaaga attagcaaaa gctacgtata cttcccattc agctatcatt cgtttatgca
    11761 aaaaaatggg attcagcgga tttaaagaat tccgtttcga gctggggcgg gaagttcatc
    11821 aattgattgc tcagttcaat caaacgattg atcctaattt tcctttttca agtgaagata
    11881 gcactcagca aattgcaaag aaaatggcgg atttatcgat ccagtcaata aaaaaagcac
    11941 aattgcagat tgaacatgaa gatttagatc aaatcggtaa agtattgaac aaggcacagc
    12001 gaatctttct ttttgcaaaa ggtgattctc aaatcacggc aaggaaattc cagaataaaa
    12061 tggtcaagtt gaataagttt ttgatcatgg ctgaagaata cagtgattct tcttggaatg
    12121 cagccaattt gactagcgag gattgcgcca tttttattag ctacagcggt cgcatccatc
    12181 attatgaacg aataatgacc tatttgaagc atgtagaggc ccctaccttg ctgattaccg
    12241 gaaatcagca ttcagaaatg gccaaacagg ctgatatgtg tcttgttata tcgcaggagg
    12301 aatatgattt tgctaaagta gcgactttct cctctcaaat tgccttcgat tacgtattaa
    12361 atacgttatt ctcagtgata tattctcaaa attttgaaga gaatatccgc aacttgaaaa
    12421 acaaacaaga attgatgcaa aaaggccttc ttaaagaata tcggaaaact taagaaaaga
    12481 tcctcattta gtaataagaa agatagtgtt tgataggctc tctttgatat catcataaaa
    12541 acaggctgaa gttttctttt tcaaacctca gcctgtttgt ttacggaacg attcgaatag
    12601 atatattata gaaatgagga atagctaact tctgtttctt attttgaacg attgacgaaa
    12661 aagatatata agaaggagga aaatcagtat acatgcagct aacaatccat acaggaaaaa
    12721 tcgtttgttc tcggatactt tctgtacttc tttttgaact tcttttgcat gataaggaac
    12781 ccggtgccct ctgacaagta agcgatgact gttgaccata taaggtgtac aagttaggag
    12841 tgtcacgaaa tcctgccctt tttcgattct cagatctttt gtatcagtcg gttctaccgt
    12901 cttgatctga tcgacttgat aagcgagtgt ccgtccattg atttcgatat agaaagaatc
    12961 acctaaagtc agtttcggta agtctgtgaa caactttgct tgaggcagtc ctcgatggct
    13021 ggaaataacg gcatgtgtat tttctccccc cgtaggataa gacgttcctt ccaataatga
    13081 ggcacctttt tctagcagca cagtattcgt ttcatcaaaa attggcaaat ggacattgat
    13141 ctcagggatc gtcaaaatac cgatcgtatg tttctcaaaa taggttctat cctttttcgc
    13201 aggttctttt ttcttagtaa agggatctgc acctggattt ccaccttctt ttgctaattg
    13261 ctggtttttc tttgtcatgt tttcttggat tttagccatg acttcttcat tttctttgac
    13321 cgcttgttgc tgataatgag agatgatctg ctgatccaga taattattca gcgcgtcgct
    13381 aacaaatgga taagctagta tccctattcc tgagagaagc aagaaaagca tcagcagatc
    13441 tagaagtttc cttttctttg gtttcatttg tgttctcctt gtgatgactg tttgataata
    13501 aaagaaaaag gagcatttgc gtaatccgct gctccttttt ccaattcttg caaaaagact
    13561 aaatctgact gtgcttgcgt ctagcaaagt acagtccagc aatcagtaga aggactactc
    13621 ctgcaccgat atacacatag attcccttac cgcctgttga aggaagtgta cctttgtgtt
    13681 tatttggtat tttttcagga gaaatcaact gtcctgctgt tacataagat tgttcatcga
    13741 tagtaaatgc tacacggttt gttaatggaa catatttttc tggcgctttc gtttcttcca
    13801 gatagtacgt gccatatttt agacctgtca catcgattaa accgttactt gtggttgtaa
    13861 aaaccgttgc tgattctttc gccgataccc agctgacggc ttttgtggat gggtcgatcg
    13921 ataaatattt cgctgtgtca ctatcttgat cacgaacgac gaattctgct ccagcaagtg
    13981 tttggtctga tgtaacgtca ccatctactt taacaaatcg tttgccccca gtaacgacat
    14041 cgactgacgg tggtgtttga tcatttgtat gcccgttatc gacatttgct tggttagaaa
    14101 atcctttggt tggatctgct ttttcgttca aatgcatata gtaaacgaat ttcaatgtac
    14161 cgccaggagt taatgaagga atataattcg gatcaactga aaccgtgaat ccgtctgttt
    14221 gctcagtgac agaataattt actgggtcga tttctttatt tccatcatat aaggcataag
    14281 catacgttcc tgaagaatca ttatcaaatg ttaaagcagc gtcatgagta tcgataagtt
    14341 tgaatgttgt gtacttgttt tgcgttcctt ctttatcagc gatacctaat gggatattga
    14401 cagaaatctc atattgaatt ttttcaccga ttgcgacatc ttttccattc aattgaggtg
    14461 ttgttttatc aactttagac gtatcatttt tgatggtctt ttctactggt gtttggctat
    14521 ttgccaggat ctcaaaaggc gtgattgttt gcttttcaat catttccgca ttatcaggag
    14581 cttttacttc ttctaaatta tattttccaa cttctaaatg attaacgatc aactggcctt
    14641 tttcaataga tgcctcggca aagtcattgt tgccgatgtc ataagaatga ccagtaatga
    14701 aatgtttggc tttggtttga tcagttgtcc aagtgtacaa tccatctgtg acactttgga
    14761 tgtatttttt gacgcttggt gttccttctt ctttagaaat aataaattct gctccattta
    14821 gtgcttcgtt ttcggcagta ccgatttttg taactttcaa cgttccatca ttaccgactg
    14881 tatttttagg gtagagatgg atagtatcta gttcttctgt cccgtattta taagagccat
    14941 ctgcttgttt gatcatctca tatacaggga aagctaaaac catgtttgcg gcagctgaca
    15001 ctccgtcttt tggttcttct ttgatcgtgt agactgcatc tttcccattt tgttttttag
    15061 gtaaagataa agtgacattt ccatcagcat ctgtcgttcc tgaagcaact ggtgtaccag
    15121 gagtcaaaga ctggactgct tgttttgctg catccacgga cgctccttta tctcgttgcg
    15181 cataaaattc ttgagtgacg ttataaactg aaaatgaaat atcggctaat ccttggtatt
    15241 gatcgaattc gctcatttct ttcccgctgt tttggattaa aggatcgggt aaatcagtca
    15301 ttttcttttt atgaaggatg acttgagctg ctgtctcctc tgcaaacact ttttttgcgc
    15361 cgaagctgtt tgtgagtaat ggtaaaataa ggaaaaggac tcctaacata acgtttattt
    15421 ttttatgatt tttcattttt tcactcctgt tctttttttg ataaacaacc agattgcaaa
    15481 gcttgcgaat accataccaa caacgcctaa aagtgtattt ttcgtttcgt ttgtttttgg
    15541 aagaaatgga cggctatttt cttttttatt gactactttc aatatactgt ccgcctcttt
    15601 atcctcagaa agaaaagttg aaatggtaaa cggaatttct gtttcactta aaatataacc
    15661 agaaggtgct tcgatctctt ttaatcgata acttcctgtt ttcaacccgg aaattgaaaa
    15721 agcgccattt ttatcggaaa tcaggtgaag cgcatctgat tcgttctttg tccacttgta
    15781 tccgttggct gtttgctgga ggtaatttcc tgctttgttt ttaataagga atactgcgcc
    15841 agctaatcgt ttatctggtt tatttgtctc gattttgaca aattgcttac tccctgtata
    15901 tacagagact ttctttgtac tgacaccaaa accaaaatcc agtgttccgt tgttgttgat
    15961 ttccttgttc gcctgtgctg tactgcttag ctgcatctga taagaaatgg tcagtttttt
    16021 tccagcatat ttttccaacg aagggatcga aaataaaaca tcaaatccgt gaggtttctt
    16081 gtgtatgaca aagccttctg tcagcttttc tccattcgat gaaatcgtta aactattagg
    16141 taaaaacgtt aatgcttcat ccgcactatc tgacaattcg aacttttgat agtcaaggat
    16201 atttacagga attgtcgtat gcaaggaata agtgattttt tcaccaatcg taaaatcatt
    16261 tctaggctct tctaatacct tctcaaaagg tggatcaggg tagtcgtttt cttcattttt
    16321 aggatacaaa tggatagttg aaagtttttg attgttttgt ccatgtacag gaagaacaac
    16381 taccatattc tctgcctttt ctttgacgac ttctggtact ttggattcaa tgaaaagata
    16441 cgctttatct cttttttctt gatctttagc ggccagagaa aaagaagcga taccatcttc
    16501 gttgttaaga gttgtagttg ttgcttctgc ggtaaacata ccggaagacg caccgttttt
    16561 tgcgatctct gcttgtgctt cttctaccgt tttgcccttt tcccgtagat ggtaaaaaga
    16621 atctgtgaca tcataaactt ggaatgtgac accatttaat cctcgatacg tttgtaataa
    16681 agctttttct tgtccgtcat ttggatgatt tttcggcagt tgcccgtttg ggaaaagcaa
    16741 tttgtgtaaa gtaatctgaa ccatttctgt ttctgtttct gttgctacct gagtaaaagc
    16801 tggtttaaat agtaacaaga agagacacat actaagccaa ccaagttttt tcatatttag
    16861 ctgccccctc tttctttttt caaaaaaaga taccaaatag aaaacgcaca accaatcatc
    16921 cctactagat agattcctaa acggccaatt cctcccgttt caggtaatgg tacttttgcc
    16981 tgattcgtga tgtctaaaga aatctggttg tttttggctc ctggtgacag aacagattca
    17041 tgatcttgtc catccacttg aattgaccca tcttcgtgta taactatagt aactggctct
    17101 tttagacctt ggtatccttc tggtgtaaaa gtttcggtca gcgtataagt tccaggtttt
    17161 aaattttcga atagaaaggt atctgtttct tgtccatctt ttggcgattc taagtccatt
    17221 tctggtccct gcagtctgaa ttttgctcct tttaatgtct gtccgttgtc ttcttttttg
    17281 tgtaccgtta gatcaaacgc ttttagtcga ttttgatgag tgagcgtcca tacattattt
    17341 gtcatctcgg tattttttcc cgtgtaaatg atttttccat atttatcgat ttggaattca
    17401 taatttcccg gcgaagtatc atatccggca ggaccagctg tttcaacgac tcgatattta
    17461 ccaggtgtga gtgaatcaaa actggcaaga cctttttcat tcgttgtttg gctgtcaatt
    17521 ggctgataag taccatttgc ttcttttttc tgtagggaaa atgctgctcc tttaagattt
    17581 atctctttgt ccgtcccttg catcgcatac ttacgtactg ctacaggaac ttcaacaaaa
    17641 ggattttcaa tagtcaactg gatcgtatca tcggaagtag tgactgtttt tccatcaatg
    17701 gttaccgtac catcagaagc gatcttgatt tcccaagtag tctttttgct taatccatgc
    17761 ccttctggag cttttgtttc tgtcagcgta taggtcattt ctttttgcaa tttgacattt
    17821 tctggaagag aataggttcc gtcgccatga tctgttagta aagtatcaat agaatctcct
    17881 gttaatttga aaacagcgcc aataagatcc ttttcacctg tagaggaatt tttcgttatt
    17941 ttcaagttta acggtgtgaa ttgtttagta tttttccatg tcattgcatc tatttgttga
    18001 gaatcgtatc ctggtacagg taattctttg attgtttggt aactgaatgc ttgaccttga
    18061 ttattgtatt gaggtagtga taatatctct tgataacttt ctccgctatt tgcagataat
    18121 ttgtcaatgt ctgcacgttc ccacgtattt gttgtatctt tagctggttt aatgattcga
    18181 atatatccgt ttttccaagc tgcagcattt gtcgtatgtt cccgttgaat ctcaaaagta
    18241 acttgatctg gacgatcagc tagattgttg tcaaattctt cccataactt tttgatgtga
    18301 agactgactc cgggagcttt agcagatggt ataccgaatt cagctaattc attggtatca
    18361 atgtttggct ggaaagttgt cctgccgttc atttgatacc agaaatttgg atggaagtcc
    18421 tcattttctg tttggattct cacttggtaa tggatttgga tttcttggtc ttttcctaaa
    18481 tagatctgat tgctcttgat ggtatttcct tctatggaaa tagatggagt gaccgttgta
    18541 ggacttgtcc ccacactctt gactgaaagt gtaccaggct gataaatgaa tggttctgcg
    18601 attggatcat ttatttgtcc attgcttaca gttgctgaga tctgtacagc ttttttggct
    18661 aggtattcag agatatctgt tgcatgatca gctgattcat agtaaagatc gcctttttca
    18721 tctgatgaaa ccatttgtcg catacgagac tctacttctg cttttgagag accagctgcc
    18781 ggatcgcttt gaagttggat gccaagacca tgtatttcga ttcctcgttc tttgagtgcc
    18841 atcgcttctc cgatggttgc gataaacgta ctgtcgattc ttctggatag attgttttgg
    18901 tcaggtgcat catagattct tgagattaga gaagtatttc ccggccgatc ttgcgtatta
    18961 gaaaactgag ttccgtaata attgctgctt gattgtgcgt gtacccgctg tactttatag
    19021 gaaaatgttg gtacaccatc cgtcagcaaa acgatcactt ttttatgtcc atttggaacg
    19081 gatagcatgc ttcctgcatc tcttagtgct ttttgtgtaa aagtaccacc atttgtccgt
    19141 gaaggtgtaa tggattttac ttgatttttc actgaatcaa atgaacccat ctgtactgca
    19201 ccgttactgt agctatatcc ttcgcttgag tagccgacat atcccatatt gattttgtct
    19261 gtgataccgc tatctgctaa agtatcgaca aaacgatcga caccaatctt tacttcaccg
    19321 atccgattat tgtcgttcat acttcctgac cagtctacga ccaatacgag atcaagaggc
    19381 gtgatatctt tttgtacatt tccacgagca ttcaaataaa catcaaacaa tccgggtgta
    19441 cttgtttcct ttgcatactt tcgtatcgca tagtctgctt gattaccggt tcctccatat
    19501 tcgatatacg aatgggtccg atcattagga tctccattcc aagaatttat accatcccat
    19561 tgatttgttc cgtttttatt gccttgatga tcaagaacat ttgtatttcc tgtaggttgc
    19621 caactggctt taggataagt gccctgatca tctgttgtat attctgtagg aatcgtttga
    19681 ttagatggca cactcacagc tggcaaggat aatgtaggca atgtaaatgg gctgacagaa
    19741 ttttcgtttt cttttgatga ttcttcttct atttcgtctg gctttggtac tgacacatct
    19801 gtatctttct tattttctgt ctcatcaata atgtcatatt ctttattatc gacgatcagc
    19861 ttgtttgcct ggctgtggta aagtgctgga tcgattggaa aactcagatc gatagtttgt
    19921 ttagtgtttg atgagatttc caacgtaatt ttcccgctat ttacctgata agcatagcca
    19981 tctccttctt ttctttccac atcacttaag taattttcga ctagaccaat ttctttttct
    20041 atcttttcag cactggtgtt attcgcattt agcttgatgt tcagtgcttt ttttccagac
    20101 tgatccgaaa tgacttcagc tgaaattgtt tctaaaactg ttggatcatc tgtagtgtct
    20161 gctattgcag ataatggcac aaagtttgat aaactcaata ttatcagcat gaaaataact
    20221 ttcagtttct tccctgtggt cgtcattttt cccctccttt ttcttttttt ctgttttgac
    20281 gtgaatagtg aaaattatta aaataaacag gcgttacata attttattta ttttaatatg
    20341 ataattatta tacgaagggc gctaaaggga atcacaccaa ttttcatacc aattggtttc
    20401 atttatgaag ttgattcctt cgattaatga gcgtttcatt gaaagaaaag ggttgaggga
    20461 gaaaaacata tgatcaggtt gttggaacga aactatagaa agatggaaaa aatcattcga
    20521 attctgtcca tttctgacaa gtggatgact aaaaaagaac ttgccaaacg aattggtagt
    20581 tcagaatcta catttgtaag gtatatcgaa gagatcaaaa tccgctgggg aacggatgta
    20641 acgattcaat cttcacaaaa attggggtat cgtcttgaac atttgaatgt accagcttat
    20701 ttaaatgttc tgatggaaat gtcgcaaagt tctacaaaca gtcgtttatt aaatgagatt
    20761 attgtcaatc cgggtaaaac gattgagtat tattgtgaaa aaatttccat cagccggtct
    20821 tcttttacac gtaagttgaa acaatgcaat caaattttag aaagctattt attaaaagtg
    20881 atcgttgacc aaggttttca gttaactagc cttaaaagtg agctccagtt gcgtatcttt
    20941 gttacctttt tctttttaag ttactacgga agacgcacgt tgccatttcc aatcgaaaaa
    21001 aagaagatca aagaaattat ggtcaagaat aattgtcaga tgaatttgtt tcaggaagaa
    21061 agcccatatg agcaatcttt ctttattatg tattttgttg tgcatctcat gagagaatcc
    21121 caaggaactc catttgaatg gaaagctgga gactcttatg tacagttgat aaatatcgat
    21181 aaaaaagact tcttaatctt gaaaaatctc ttaggaaaat tgaagtacag cagctatata
    21241 aaagtagtga actattttgt caatacgatt ttttcagaat tttcggagaa agaaaaaaat
    21301 gaaatcagtg agcagatacg aaaaaatatg tcctactgtt cgttttcatc cgctgattgc
    21361 aaagaaaaaa acgaagaata tgttgttcaa ttattaacgc aaatttattt tttctcccgt
    21421 attttcccat acgatacgac tcatttgacg atccgtgcaa cttactttgc acagcagatg
    21481 caacaccgcc agatacgtct atatgctgga tttcgtgcag aacttcaatt ttattccact
    21541 ctactgaatc agaatctgat ttattattta tctagctata tcttttggat gcttatcggt
    21601 aatcctgaaa ttcaccatta tacttctgat aaaaaaatac tgattatcag cgacttaagt
    21661 cttcgacata gtcaatatat tgaagaatat atcagtgata ttcttgctgt gcataaaatt
    21721 cactctgaga caactgctat tactgaagat cagctgtcga aatacaatct tctcgagtat
    21781 gatctgatcg ttacgaatca acccattttg aatgcccatg ttcctcatat attgattgat
    21841 gatagtgtgt cattcgcaaa tgaagaagaa ctcatccgct tattcgagtt atgaataatc
    21901 aaaaaacagc tatgtataaa ccaccctgat aaaaatttca ggacaaatac acatagctgt
    21961 ttctttttat tgtattcctt cagcaatcca acgagattta tagcgaaagt cttttaacaa
    22021 ctcttctttt gtccaattat ttgcaatgag atccgtcaaa gtgttcatta cgagggtcat
    22081 ggaaaagtat aggaaactaa ctgcttcttc cgacgattga atggagaaat gattgattct
    22141 taataattcc agccattttt cgttcattgg agaatcagcc gatagttctc tacgtttggt
    22201 tgtctgctga tcatttcgct gggttaacaa gtataacctt tgccaataga tactttcgtg
    22261 atctaagtcg cagcaccact ctaaatattt ttcgatcccc aaaaagaaat gttgtttgtt
    22321 ttcgctaatg aattttaaga tatcttgatg gactatgtga gaactgcttt tgatcgtata
    22381 caaataagca tcttccaaat cttgaaaata tttataaaaa gcaccgcgcg acatccccat
    22441 cttgtcaacg atttctgaga ctttgacttg gctgatgtgc tgtgagcaaa atatctccag
    22501 taataggtca tcgattctct gtttctttgc gttcgataaa ttgaaaaaag tttcttttgg
    22561 catccacatc tctcctttat ctctgattat acgtggatag cccttcattg tcatgaagga
    22621 ttcattcctt ctttttctct tcttctgtca agtacccatc ttccattcga taaacgcgat
    22681 cactccattg aatcatacgg acatcatgag taaccataat agtcgctttt tgtttctcat
    22741 gagcttcttt aactagtaat ttgacgactt catatgcatg atctgtatcc aaactagctg
    22801 ttggttcgtc tgctaagatc aagcttggct cattatataa agcgcgagca attgccactc
    22861 tctgtctttc tccaccagaa aggtctttag gataactatt tttaagtgac tggatatcta
    22921 aagattgcag caaggaatcg atttttgttg aatcttcttt ttttgaagta acttggtcaa
    22981 ctagttcaaa ttgttccttt accgttaaaa aaggaatcaa attagagctt tggagaataa
    23041 atcctatttc tttaaagcgt aaatctgctc gttttttttc tggaagctca gttagtgagt
    23101 gaccgttgat cactatttct ccggaagtcg gtgtttgcaa tcctcctgaa atggtaagga
    23161 aagtactttt acctgaacca gaaggaccaa tgatgctgac gaattctcct tcctttacat
    23221 aaaaatcaat tgctttcaaa gctgagacct ttgtatgacc agaaccaaat gttttggtaa
    23281 cttttttcat cgttaatatt tttttcattt ttattaacct cctattgctg taattgggtc
    23341 tactttggtt atcgtacgta tcgaaaacaa tccgcctagc attgcgacaa tgatcaaaat
    23401 cccactatat agcaaccatt gtgaccaaaa aacagcaaaa ggcatcgcgc ttggtaaaat
    23461 cagactagtc aaataagcaa acaaaagagc aagcagaacg cctaccaccc caacaataaa
    23521 ggactgtgct actaaagatt tagcgatgaa gaaatttttg atcccttgtg ctttcattac
    23581 tccaaaaata gcagtcttct gaagtgtcat cacgtacatg aaaataccga ccacggcagc
    23641 aaccactaag aacaaaaagt atatcatcgc atttaaggtc atattttggg cagaatagcc
    23701 aggaatactt tcaatgaatg tcccaatagt taataactgt agacccttcg atgctttttc
    23761 tttcgaaatt ttagctgaat cctttattac gatcccattg atcggctgtt cttcttcaga
    23821 agcaaacggc tgttgtccat acttcaaagc agtccacgta tctagggacg catagatgac
    23881 gggagaaact gtgtaataag tttcaggaaa gataccgaca atcgtcaaag gatcgtcgta
    23941 actgccaatt ttgatcgtat ctccaatttt atagcctgaa tcagccagat tttgagagat
    24001 gatgatttca ttctttgttt taaattcatg ccctttcgtg agttttggta ataaaaatgc
    24061 ttctttcgtt gtgccgaaaa ctgtaacatt ttctttgttt tttcctttga ttgctccgct
    24121 gtacaatccg attggtgctt tctctcctcc ttcaatgtat ttcagatctc ctcttgttaa
    24181 ttgtgaagct gcaaatactt gatttgcatc ctccgacaaa acaatctctt gtgcgtccca
    24241 atcatcgatt gcttttttaa actcttctga aagtccattc gctagtcctg acaacatgaa
    24301 tacgacatac gcaatcaaga acataatacc gacgatcaag ccatatcgta atttagaata
    24361 gcgaatttct ttgatagcta gaaacatttg tttttcctcc ctatgataga ttgatagtga
    24421 caaaatgtca cttgttgaat agtgtaactc taaagtgaca atttgtcact tgtgttgttt
    24481 ttttcttatc aataaattgt ctaagacatc tttcgataaa accttaacat gagtcataaa
    24541 aaatttattt aagccttatt tatgctttcc atgataaact aaagtgacca aatcaatgag
    24601 gtgggaaaat gattaaacta ttagtagtag aagacgataa agtattatcc aataatataa
    24661 aagaaatact cgctgcaatc ggcgagataa cacaagttta tgatggtagt gaaggactgt
    24721 tcgagttgga aagtggagtt tatgatcttg caatcttgga tttgatgatg ccggaaatga
    24781 atggatatga agtgttagca aaaatacgga aagaaaaaat acagactcca gtattgattt
    24841 tgactgcaaa agatagtttg gaagataaga ttgaaggatt tgaacgaggt gcagatgatt
    24901 atctaacaaa acctttctat cgagaagaac tgatcatgcg cgtgaaagca ttattgaaac
    24961 gctcgctagg tctctttgat gaaaatgttc tgaagtataa ggatatcagt tgtgatttaa
    25021 aaagcaatga agtcttgtat aaagaacaag ttttgcccat acaaggaaaa gagtttgaat
    25081 tgctcgttta ttttttacaa aataagggaa tcattttgac aaaagagcag atatttgacc
    25141 ggatctgggg atttgattct gatacgacga ttacagttat tgaagtttat atgagcaact
    25201 tacgaaaaca tctgcgaagt actggattgg ataaagaaat caaaacctta cgtaatgtcg
    25261 gttatatact gcaaagggtg tgatgcaatg aatggagata ttacacgaaa gcaacgtagt
    25321 cgcttttttg cgacaaatgt agcagcattt gcttttatct tttttctttt gggattcatt
    25381 actttacaga tattaaacca atctgcatac agagaaacag atctgtcatt agagaaaata
    25441 aattttgatt ctcgaacagt acaattagag atacaaagat atcagcaagg cgatcccttt
    25501 ttaaacccga ctcaaacgga acaaaacaga acaaacatgg aacagccatt aatggaacca
    25561 tcaaatgacc gttttaacac tcaagtgatc ttgtggtcta aaaatggtga gatattgaat
    25621 aaagcagcta ttggtgggcg tttcaaccaa atcaaagaat taaaaatgga tacgaaaaat
    25681 ctgaatacca tccaaaatat tgaattaaat gaagacagtg gctcatctgc cctctctttc
    25741 cgttcgatta cgaaaaaagc ggagacgaat caagatggcg ttgcctacat ccaaatttta
    25801 gaaaatacaa atcaaatcaa gaattcatta gcgacttttc gcacgattat gattttgtgt
    25861 atgattgttt tttggattct ctctattggt atcagttatt acttgtctag tctaagcatg
    25921 cgccctatta ttctttcttg gagaagacaa aaagaatttg ttgagaatgc ttctcatgaa
    25981 ttgcgcacac cgttaacgat tattcaaaat agtttggaac atctttttac caagcctaat
    26041 catactatta ttgaagaatc cgaaagcatc gctcaagcat tgtcagagac gaggcgcctc
    26101 actggattga cttcggattt gctgacaatc gctcggaatg attctgatca gcagctgctt
    26161 tcaaagcaaa tgatcaatac acaagaatat atcgaacaac ttgttaaacc atttcaagag
    26221 atggccatca tagatggaaa agagtttatt ctccataacg atgcaacatt agaagtacca
    26281 attgatgaaa aaaaaatcca tcaagttttg gttattttgc tggataatgc attaaaatat
    26341 acgaatccag aagataagat cattttggat tcatctgtta accataaaga atggctgatt
    26401 cacgtaaaga atactggccc ttctatcagt gaagaagatc aagcaaggat ttttgatcga
    26461 ttttatcggg aagatcaatc tcgggcgaaa gagactggcg gatacgggct tggcttggca
    26521 attgcaaaac aggtaatcga acagcatcat ggacagattt ctgtatccaa ttatcttcca
    26581 caaggagtcg attttaaagt ccaattgccg ttgaagccta aaaaataatg cttatcccta
    26641 aaaaagggct gtgacaaaaa tcttgtcaca gccctttttt gttgcgaata aacggtgttc
    26701 cagaagtagc ttcttcggaa ataagccgaa atttctttaa gctgaccact tctgtcacaa
    26761 cctctttagc ttacgcttac gattttttat tgcggtcttc aatgtgtaca tcaccgcttg
    26821 tgtcaatatc caattcttct cggctaacat cttctgtcac tttttcaaca tcttcatgtt
    26881 cttctttatg aatcccaact tcttcacgaa taactgtatg tttgcttaca tctacttgct
    26941 cttctttgat tgggatagta atcgtgtcat cttccaattt ttcgtctgtt ccctgtgaag
    27001 attgatctgt tactggtttg cgttcgatga cgatttcttc acgtttgacc ggtacttcta
    27061 ccgtttcagt atcgttgacc acatgtttgt ggatgtcgac ttctccagtt gtcacatcat
    27121 gtgtatttac atctaatttt tcttctttta gttgaattgt atcatcatct agatttggtt
    27181 tgcgatcgat gtcttcagtc ttgcttgtac ccattgtatc agtctcgcca ggtacgtcca
    27241 tcggatcagt tcctggtgtc atattcaatg gatccgttgt attttgaaag cctgtcgtgc
    27301 cacctacgtc cattggatct gttccagtgc cagtttctaa tccagtcccc attgtgcttg
    27361 ctgggctaac agtatcttgt gtatccattg tatcttctgt tgtttctggc tgacgatagc
    27421 cttttactgt gatgacaaat tttccatcag aaatatctcc tctatattga tataaaggat
    27481 cattttctgg tgtagccgtc tcagaatcat aggtatcaaa agaaaaagta tcttttattt
    27541 tttcccataa agaacggtct tcttcaccat ccatccgatt ttcatctgtt tcaacatcta
    27601 taccagacaa tccgtcaaat cctagtctag atgctgcttc atcagtagta taaataacga
    27661 tatcatctcg ttcataccct tcgttacgca accgttcaat tactgataat gcttcttccc
    27721 tcgtactata gcttccgatg accgttaaat ttttctcttc catgtaaatt cctcctagca
    27781 aatagattta tgattggtat attgttactt ataaatttat tctataggag acgattgttt
    27841 tctacaaata tttagattgg aatttgatta aaacaattta attgaaattt gaaaacattt
    27901 atgttaatga aagtcatatt ctaataaaat gtcatttgtt tctatatgaa tttttatgaa
    27961 aaatttcata taatttccac tcgttgtgat ggtttaattt atccaatcca tcaataagca
    28021 aacaacttat ataaaaaagg attaattctc aatataattg ctcctaccat agaataaatc
    28081 ttcatgtaaa gtaatgaagt accaactatt tctaatactt ctttaagaat tatcccaaca
    28141 atatcgttag acaaaccatt attgaataaa tggagagcca atcgattctg aaaaaatgtt
    28201 ttctaatttt tcggaaataa aagaaagagc ctcctgccat tagaacgaac caaatccatt
    28261 tcattgctca tgaagtaacc acatagctaa aaagaggata aatcaattaa aagatcaaaa
    28321 gaatgactgc actaataaaa taactaaaaa tgtatcttct tacttcatat cttttagcta
    28381 gtacttagtt aaaaatttgc ttcctaagat caagacaaac tgtctatctt catttgatga
    28441 aatgcggtaa aatatagaaa atgaacacta ttggagggaa tttgtaatgg acagcaaaac
    28501 tagaaaacaa ctgcaagaaa agttaaagca acgtacacac ttgctccctt ccgtctttca
    28561 acagttttta gaagaaaatc gttcttccct ttcattaggc agtcgctatg aatacgcaaa
    28621 agattttcat ctgtttactg attatttgag aaaagtattt tctgaagaag tggatgatca
    28681 gctgattatc catttagaaa aacaagatta tcagaatttc ttagctaaaa tctatcgaca
    28741 tactcgttcg ttccaaacta cagcgaacca agaaacggag caattgtttg aaaatagcta
    28801 ttacgggatt tcaaggaagc aatattcatt aaatcatttt ttacgatttt tgtatcaaaa
    28861 aggactgatt gaagaagaga tttctctttt gggtacaagt ttaccggaaa caaccaaagc
    28921 agctccgcga tatatcgatg cagaactttt caaagatttt gagcatttgt ttatcccgat
    28981 tgaaggcttt caaacaagca gagaagctag ctttgaagaa aagaatagac cgaggaacct
    29041 tgcaattact gccattcttc tgtattgcgg attaaagatc catgaagtag tagaacttaa
    29101 acgtaaagat gtatctttga ccaagcagat tcttaatctg aatagaaaag aaaacagact
    29161 gaataaagta ccaataccaa aagaagcgat gccatttctg gagaactatt ttaagttaac
    29221 agcacaaaat acggacgaaa attcttttgt ttttcgttcc tcacatgatc agcaaatttc
    29281 tcctcggact attcgccaaa ttttgagcaa aattacagta tataaaaacg tatcgccaga
    29341 actatgcaga aaaacatatc gttattatgc agaactatat ttagatgatg ctgacgcggt
    29401 aaattattta tgtggtaatc gatacctctc atcccattct tttcaagata tctgggaaaa
    29461 gatggttgat ttttcttatc acgaattagc ggcattcgtt cgggtttctt ccatataaaa
    29521 caaatgtatg atcttttttt cacaataaac tatgttatta tataaaggat tatgttacga
    29581 ttatcttgaa tttgtatatt acttaacaaa agataatgga gtgtgaatga tggagaccat
    29641 aacgtattcc cctttgaact tatttacgaa ttttcaagaa gctgcagcta acttttccga
    29701 gaccccgatt atctttgatg agccactttc agctttccca gaattaggac ttgaaacaac
    29761 ttacaaaaaa tgctgcgaaa gtgtgttgaa acgggcttat caattggctc atcttggagt
    29821 gaaatccgga gacaaagtta ttatttataa gagttccgct tttgacacat accttctagc
    29881 tgtttctgtg tcttaccttg gtgcagtgcc tgtcatgact tcttatcatc taccaacaac
    29941 aacaatggaa gtatttatcg atcgtttaga agaccctttc attttatttg atgatgagac
    30001 aaaaaagcga gtgagagaaa tttcaaatgg aacaaaatca aaacagattc
//
LOCUS       DACEPD010000028        28258 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.028, whole genome shotgun sequence.
ACCESSION   DACEPD010000028 DACEPD010000000
VERSION     DACEPD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.028"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(425..994)
                     /locus_tag="IW797_001180"
     CDS             complement(425..994)
                     /locus_tag="IW797_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1223967.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1068..2357)
                     /locus_tag="IW797_001181"
     CDS             complement(1068..2357)
                     /locus_tag="IW797_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223968.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2711..3559
                     /locus_tag="IW797_001182"
     CDS             2711..3559
                     /locus_tag="IW797_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ1223969.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3573..4646
                     /locus_tag="IW797_001183"
     CDS             3573..4646
                     /locus_tag="IW797_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ1223970.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4636..6807
                     /locus_tag="IW797_001184"
     CDS             4636..6807
                     /locus_tag="IW797_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAQ1223971.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6919..9090)
                     /gene="copB"
                     /locus_tag="IW797_001185"
     CDS             complement(6919..9090)
                     /gene="copB"
                     /locus_tag="IW797_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ1223972.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9110..11296)
                     /locus_tag="IW797_001186"
     CDS             complement(9110..11296)
                     /locus_tag="IW797_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ1223973.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11296..11505)
                     /gene="copZ"
                     /locus_tag="IW797_001187"
     CDS             complement(11296..11505)
                     /gene="copZ"
                     /locus_tag="IW797_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ1223974.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11518..11958)
                     /locus_tag="IW797_001188"
     CDS             complement(11518..11958)
                     /locus_tag="IW797_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ1223975.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12032..12505)
                     /locus_tag="IW797_001189"
     CDS             complement(12032..12505)
                     /locus_tag="IW797_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HAQ1223976.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(12815..14281)
                     /locus_tag="IW797_001190"
     CDS             complement(12815..14281)
                     /locus_tag="IW797_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1223977.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(14592..16676)
                     /gene="cadA"
                     /locus_tag="IW797_001191"
     CDS             complement(14592..16676)
                     /gene="cadA"
                     /locus_tag="IW797_001191"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ1223978.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            complement(16818..17006)
                     /locus_tag="IW797_001192"
                     /pseudo
     CDS             complement(16818..17006)
                     /locus_tag="IW797_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(17200..17559)
                     /locus_tag="IW797_001193"
     CDS             complement(17200..17559)
                     /locus_tag="IW797_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ1223979.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(17589..17930)
                     /locus_tag="IW797_001194"
     CDS             complement(17589..17930)
                     /locus_tag="IW797_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223980.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(17927..18601)
                     /locus_tag="IW797_001195"
     CDS             complement(17927..18601)
                     /locus_tag="IW797_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1223981.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            complement(19396..20694)
                     /gene="asnS"
                     /locus_tag="IW797_001196"
     CDS             complement(19396..20694)
                     /gene="asnS"
                     /locus_tag="IW797_001196"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ1223982.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(20734..21924)
                     /locus_tag="IW797_001197"
     CDS             complement(20734..21924)
                     /locus_tag="IW797_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1223983.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(21945..22472)
                     /locus_tag="IW797_001198"
     CDS             complement(21945..22472)
                     /locus_tag="IW797_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1223984.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(22519..25308)
                     /locus_tag="IW797_001199"
     CDS             complement(22519..25308)
                     /locus_tag="IW797_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1223985.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDSSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAIYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKEIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(25455..25655)
                     /locus_tag="IW797_001200"
     CDS             complement(25455..25655)
                     /locus_tag="IW797_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1223986.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(26107..26427)
                     /locus_tag="IW797_001201"
     CDS             complement(26107..26427)
                     /locus_tag="IW797_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223987.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(26728..27846)
                     /locus_tag="IW797_001202"
     CDS             complement(26728..27846)
                     /locus_tag="IW797_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223988.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
BASE COUNT         7968 a         5993 c         4938 g         9359 t
ORIGIN      
        1 cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta actttaactg
       61 ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac aatcggaata
      121 gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt gccattccaa
      181 gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag aagagcaaat aaaaaagcac
      241 tcaaattgag tgctttttta agctcactat cactaaaact atcactaatt ttatttataa
      301 gcgaacatgt ttctatcact gattttttaa atctagcgaa aaacccttat cttaaaggcg
      361 ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg cctagaagtg cgaacatggt
      421 tttcttatag ctcatgtaga aaaattttct tgatgatgat tttatccatc aattgagatt
      481 gactgttttg ctctcctatg atttcagcta tgattgtccg attttgcggt tttcttctta
      541 actggtagtt gattttccca gtttccatca actcgctaag catatccacg actccgccaa
      601 atccggtctt tttcgtaata aaacctagtg ctaccattcg ttcatctaga ttttttgctg
      661 taatcgtcac accagtaatg agcgagtgct gtaatgtttg ggccagctct tccatccctt
      721 cagcattgga tacgacggaa gctgttttgt taataggttc ttcgtttatt tcttttggtt
      781 ctttcctctc cactttcact gtttgacgaa ggacatctag ttcttgcaag agcgtttcgt
      841 attgttcttg ggtcagcatc acgccagcta ctttttcacg attaaatata tatacgccag
      901 ttcccgcgtc tcgcgcttga gcaaatacat ccatcggcga tcgtttcact tctgtgatcg
      961 aagatgtggg aacttctaat ttctttaatt ccaatggatt tcctccttta ctaatcgctt
     1021 tttattatag cacagactga cttgttttga tcttttttcc aaaggtttta tcggtttttg
     1081 cctaaccagt aattgatatc ggtaagaagt ctttcgatac caaatgaagg atggtacaga
     1141 tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat gatcggtgat cactagatct
     1201 gcataccgag gcggcttatt tcgagtacat acatgtatag agatccgttt tcgatgtctt
     1261 agcgttttgt tcagtacccc agcaagtctt cttgcttctt ggtgttcata gcgaaaaaaa
     1321 aagacgattt tcacttcagg agggtggaca tctaaatacc ctcgttcctg gagtgttttc
     1381 agtaaaagca tcccccactc tactgcgggg tgtgcaaatg cttgttggat cgctagccga
     1441 aattgatata gtttatcttt ggaattggga aatttttcta atgtttcctg ataataacga
     1501 acttcttcta tcagccggtc atggatacca attacgtaac gaagccattc ttggtaaagt
     1561 acttttgtca gctttgattt gttttcgctc tcaaattcgc tattcagcca aaacagcgaa
     1621 tcaagctttt cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc tatccgtttc
     1681 caccgctttt cagtaatctg gttacagctc aagcgcaaaa ttgctagata atttttttct
     1741 tttattggca aaatctgctc ttctctaaat agcaattcag gaaacaattc aaagaatagt
     1801 gttcgactgt ctcctaaaat ctcgatttct gccggatcct ttctttgctt caataattgg
     1861 atgaacatct cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg aaaatctttc
     1921 aaaaagcaaa acagcagata tcttactttt ctttctgaac caaagatctg tgctttttta
     1981 taagaaaaag aaaggtggta ctcgccaaac cactttctca atcgctcttg cgtagaacga
     2041 tcgtacttct gctcgtaagg ttcattcaat agcagatttt tcagtaaaca aaacgggggt
     2101 gattggatca gaaaaacaaa caagagataa ggttcgctat atccttgatc ataaactaac
     2161 tgaacggttt tcttatgaag aatcagctgg atgggacagt cgctttccgc caaacgtttc
     2221 agatgccgat ccactgtttt aacatcgatt ttcagttctt cagctaaatc agcttttcgc
     2281 cagatcaaat ctttcattag attgattagt gaaaataaat tctgtgcatt ttttgataat
     2341 agtttttgcc atctcatcga tcatttctca cctgatttct aaacatattg tttgatcaaa
     2401 atgggttcac tcctaaaaag ataaaaaact ccctttctat ttatttaaat acgcaaattt
     2461 tcctcattat ttttaatttt actcaattta atcgtattct catttcaaaa cgaatgtact
     2521 ttaaaaaaac ataataaaat cactttgctt gtaggatgta ttcccttcca ttacaatgaa
     2581 ataaatagca attttatgag aaggtgatgt gaaaatggaa gaaaaaatcg aagaatccaa
     2641 acaaaaaatc gatcagttga tacaagaatt agaaaaagcg gtcatcggca aacgttcaac
     2701 tatccaattg atgatcgtgg ccctattagc aggtggtcat gtcttattcg aagatattcc
     2761 cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa gctgtacagg gaaccttcag
     2821 cagagtccaa tgtactcctg atctgctgcc tagtgatatt cttggttttt ccgtatacaa
     2881 ttcacaatca aaagagtttg aatttcgtcc gggaccgatc tttaccacga tcttgttggc
     2941 agatgagatc aatcgcacga ctcctcgtac tcagtcagca cttttagaag caatggccga
     3001 gaatcatgca acgatcgaca acaatacata ccctctagac aatcattttt ttgtcttagc
     3061 tacgcaaaat ccaatcgagt atgaaggaac atatcctttg ccggaagcac agctcgatcg
     3121 ttttttattc cgactacaaa tcggttatcc ttcttttgat gacgaacttt tgctactttt
     3181 agataaattc gaaaaagtgt tggataactt aaatgaagta ttgaacagtt atgaaataga
     3241 agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc gaagtggcat cttatgcctt
     3301 gcagttagtg tcagcaacaa gaagccatgc tgccatccag cttggcatca gtccacgagg
     3361 aagcctttcc tttattcagg cagcaaaagc ttatgcttta atccatggga gaaattacgt
     3421 cacgcccaaa gatttgcagg atttagttcc ttacgtgttt tcccatcgcc tgatttttta
     3481 cgatcgttca ttgaaggaag acgaaaaaaa acaaatcatt catactcttg ttgcacaaat
     3541 tcctgttcct gtgaggtaga ttgaggtaga taatggaaaa aatcaaagat tatttcatta
     3601 attttgtgtt atttctggta tatttcatca ttgtgctttt tgctcttgta ttcaacaatt
     3661 ctttaggctg gttcttgttg gcctttttta ctctttatgt atcatggagc ttgatttcat
     3721 taagcagctt tctaaagaaa ctttcggtta cttgtacatt gcagccactc gtttatcgca
     3781 aaaaaagtat gcaggttatt ttttccatag caaaaaagag aactttttct ttacctctcc
     3841 ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa aaaacaagag attttgattc
     3901 ttacaaataa accgcaagaa gtccaattgc tttggcagcc aaaagaaaga ggatgccatg
     3961 aaacattaac tgtcattttt acagcctatg atagtctagg acttttcaga aaaagtgcaa
     4021 aaagagagat ctcttttcct gtgactgttc tgcctgcttt ttgtaagatt caagcagaaa
     4081 aattacagag agttctggaa aaaaaacagc agctgaacgc gtaccaaaga ggtcttgatt
     4141 ttcgggaaca tcgcgcttat cgtccaggtg attctttcaa ccgaatcgat tggaaattgt
     4201 ctgctcatac tcaagagtgg ttataccgtg aatacgatta tcaagaagaa gaatgttctc
     4261 ctcttttttg cttttggggt tcgcacagtc caaaatttga acctctgcta gacttgtatt
     4321 tttctttatg gcatttgaga aaagaaaagc agccggaact tctgatatta ggcaaccgcg
     4381 cctttcatgg caaagacccc ggtcatacct attttgcttc tttggaagct ggcgatgaga
     4441 aagctttttt cgcacaccta aaaaaatatg caaaaaaaca aatcgtcctc tttgttcctg
     4501 agaattctcc tgaagtctca gaagctattg aaacattgtc aaaagatcga acggtttatc
     4561 ttgtttattt cgtagaaaaa caactcatgg tccaagaaaa agacaagata tgttcattgc
     4621 caggaggtga atggattgac gattaaatgg gaaaaatggc tgtgtggcgt ttactccttt
     4681 gtgatactaa gtgtaggtgc tccgatattt gtagtagcta atcaattgca aaacagctac
     4741 cttcttatcc taactattgg tgtcctatgc cttattgtct gctgtattcc taaaaaatgg
     4801 gtacgtttgc tgctttcttt gccagtaatg ataggttgtt tgtttttgta ttttccttcg
     4861 aagacatttt ccttattatg gatcttacag tttttttcgt cagtcacaga ggaattcttc
     4921 catctatccc aaggtaaact ttattatttg ccagaaaaga cagctttttt tctcataatg
     4981 ttgctgatct atttatttat tacattgacc gtggattacg actatttgta cgcacccttt
     5041 ctttctttga tggcttactt catgatgctt accgtttttc gggaaaaaga tttactgtac
     5101 gaaatgattg cggtagtaac tgttttgttc gtgtatctgg cagcaaggca atttttctcc
     5161 atgcggttgt tgtcgggaaa cactcgattc cagtcattga ctacagttct gcttatttgt
     5221 ttcttaacta ttagcgctat cttgccttta tatcttccga aagtacacca atctctcgag
     5281 gaaacgagtg aaccgatccg agaatatctt aatgatgaag ggctttatga tacactccat
     5341 gagtatcaat taaccggaag tgcaagaaca ggcttcagtg agaatgatga acagcttggt
     5401 ggacctttat atgataatgg cgaagttgtt tttacagcga accaaaaagg aaatggctat
     5461 tggaagatcg aaaataaaaa tcattacacc ggttctggat ggatagaaga tcaggacgca
     5521 cccatccaac caatccaaga attccctgtt gacatcacga gctaccaaac cgttatggaa
     5581 gacaaagaaa acattacatt gaccctaaaa aaacaagctt tcttacctta tccttatgga
     5641 aatactacct tgactgattt ttcattagct agtggacaaa cgctttacta tttgcctgaa
     5701 agccagcgat tcattcctgt cccgaatgaa tcgacacgtt ctgcaacttt acaaacagaa
     5761 aggttagttt ttacgagaga agaattaaaa agcagtcagt taaaagctga tgatttagaa
     5821 aatcagcaga caaatcttca attgccaaaa agtttgccta aacgtattga ggacttggcg
     5881 gtcgaattga ccaaaaatga agcaacttta attcgtaaag tcgaagctgt cgaacagtat
     5941 ttaaaaacaa gcggaaattt ccgctattcc aaaacggata ccggacatac tccgaaagaa
     6001 cacgattatg tggattattt cctctttgat tcaaaagtcg gctattgtga caacttttca
     6061 acttctatgg tcgtccttct tcgaacactt ggtatcccag ctcgctggac aaaaggtttt
     6121 acaaatgggc tcagaacagg cacagataat caagggaatg aggaatacag cattctcaat
     6181 agtcatgctc attcttggcc agaggtatac tttcctggat ttggctggct tccatttgaa
     6241 ccaacccctt catttgccaa tccaatgggt tcacaaccag ttgctgaaac aaccgaaacc
     6301 gtccagcctg ctatcgaaca accaaatgaa agcgaaaata tggcagaaac tatttccagt
     6361 gagagatctg aggtcattcc atcagaagaa acaacactca caccagctta taaaatcgtc
     6421 ttattcagcc ttggggtgtg tttatacgtc ttgttgttct ttctattgtt attcagaaaa
     6481 tctctgcgct ggacaagtta tcttatctat cttcattttt ttgcttctac taatacgatt
     6541 gcatatcaaa aacttcttaa ccatttggaa acattggtac cgcgcaaacc cgcagaatct
     6601 ttgcaggaat atagcctgcg aatagaacaa gaattcaagc tagatctgcg tttcattcga
     6661 ttaactactc aatatgaagc agaaatatat ggacgacaag cagatccggt aaatttcgat
     6721 aaaaaaacct atttagaaat tacgcatata ataagaaaaa cggtatacaa aaaagttctg
     6781 acaaaaggga gccgcttcaa aaaataagat aaaatttctg gagaatctac ttctggaatg
     6841 ccgagaacca gctgcttgag tatcgattat tcgaaaaaaa ggttgtgaca cgttttttgt
     6901 cacaaccttt tttcattatt atttgatttt caatgtcaaa gcatttagag ctacaactac
     6961 cgtacttaac gacatcaaga cagcaccaac tgctggactt aatatgatac caattggcgc
     7021 aagaattccg gctgctagcg gaattgcaag aatattgtaa cctgcccccc accagaggtt
     7081 ctggatcatt ttctttcgcg tttgcttggc tagatcaaga aaatgcaaaa tatcttttgg
     7141 atcgctgttt gtcaatacga catccgcaga atcgattgcg atatctgtcc ctgcaccgat
     7201 tgccatcccg attgaagcac gggctaaact tggcgcatca ttgatcccat cacccaccat
     7261 gattactttt ttcccttggt caaggtattt ctggacaaca gcttctttat catctggtaa
     7321 taaaccgcca taatattctt cgatccctaa ataatctgct actgcttggg cagcgttttt
     7381 attgtcacca gtaagcatga caggagtgat ttttctttct ttcaatgttc ggataaattc
     7441 ttttgcttct gtttttacta cgtcacctaa agcaacgatt gcagctagat gattttctaa
     7501 aatcaggaag ctgatggtat ttccttgttc ttggtaaggt ttcaataatg cttgttctac
     7561 gtttaatccg agacgctcaa cttctttttc attaactatt ttcacttgtt ggtttttgac
     7621 tgttgcctct agcccaactc ctgaaagatt ttttagattt tcggcttgat aaggcttgat
     7681 ttcatgttct ttcaaataat tcataatacc aacagccaat ggatgattgg cattcgcttc
     7741 caatgctcct aaatattgaa ggatttcatt tttattgaac gcatcatcaa gtacttcgac
     7801 acccgttaca gtaaattttc cttctgttag tgttcctgtt ttgtccagca tgatcacatc
     7861 tagatcattg gcttgctcca gtgcattgcg gtttttcaaa agtaatccgt ttttagctgc
     7921 tattgatgta gagcgagcaa caactaatgg gatagctaaa cctagagcat gaggacaggc
     7981 gatgataaag actgttacca tacgttctaa tgcattagga agatctgtca agaacaacca
     8041 ggcgatgaat gccaggatac ccacaattaa tgcgacataa aacagccatt ttgccacttt
     8101 gtcagataat gactctagct tggatttttc tccttgtgct tttcgcacca tttccataac
     8161 cttcgctaga taaccatttt caccagttcc agttacttcg atttcgatcg ttccatcccc
     8221 attgatagaa ccgccaatca ctttgtcatt ggcttgtttt ttgattcctc ttgattcgcc
     8281 agtcacagct gattcgtcaa caattgtttc acctttcaag attacaccat ctgttggcat
     8341 tttatcacca gaccggacga tcaaacgatc accttcgttt acttctttca atgaaactgt
     8401 ctcttctttt ccatctttcg tcaagcgttt tactgattct ggcagtaatt ctgctagttt
     8461 ttgtaaagca ttgctggcat tcgacacagc attcatttca atccagtgac ctagtagcat
     8521 aataacgatc aaagtagcca attcccagaa gaaatccatg acgtgtgtat gtggattgag
     8581 cagatttgcg ataaacgaat agacactata aatatatgca actgtaatcc ccatcgcgat
     8641 cagtgtcatc atagcaggac ttttttgttt caattccatc ttagctccac ttaaaaatgg
     8701 ctgaccgcca tagataaata gtatagttgc taagatcaat acgagccaat cagaaccagg
     8761 aaacgttact tgaaatggaa attccattcc catcattggt gaaaaaagaa tgattgggat
     8821 agctaggata agcgatagcc aaaatttttg tttgaaattt cccatgtgca tggaatgatc
     8881 catgccgcca tgatcgtgat ccatgtgcat cgaatggtcc atttgtttgc tgtttgctat
     8941 atctccatgg ttatgtccca tgtgcatgga atggtccatc tggctatggt catgtttcat
     9001 ctccatctgt ttttcatgag attctgtact ttctttgttt actacttctt ttttatgttc
     9061 atgttttttc tccatatcgt tcccattcat ttttactcca tcccttctct tactttttca
     9121 tgtgccgatt taagctcagg gagttcaaca atacactgac ggaactaaac gccatcgctc
     9181 ctcctgcaat gattggattc aaaaagccta gtgcggcaaa aggaatacca atcgtattat
     9241 agacaaaagc ccagaaaaga ttttgtttga tttttctcaa tgtcacccta gacaactcga
     9301 ttgtttgtgc aatggatgcc aaactgctac tcatcagcgt cacatcggct gtttccatcg
     9361 cgatatccgt tccacttccc attgccatac caacatcagc taatgctaaa gccggtgcat
     9421 cattgattcc atcgcctgcc ataccgactt ttttgccatc tttctgaagt ttttctacgt
     9481 aggctgcttt atcctctgga agtacttcag caaagatatg ctttggatca ataccaactt
     9541 gtttaccaat cgtttcggct gccagtttgt tgtctcctgt cagcataaaa acatctaatc
     9601 ctttattctg gagctgcttg attgcttgtt tggcttctag tttgatttga tcagccacgg
     9661 caatcaaccc gatcacttgc ttttcatcag ctaaaaacat gactgttttt ccctgctgtt
     9721 ctaattctaa cgcgtgttct tggtattcat caaatgatag gttcaattca attaatcgtt
     9781 tacgtgtacc cgcaaaatag cgtactccgt taatcgtacc gctgattcca gcacctgggt
     9841 gagctgtaaa atcaatcata tcgtatgttg ctgcttcttg cagttttccg tattccacga
     9901 ttgctttacc tagtggatgt tcagaagctt gttctaaagt ataaaaaata gataggactt
     9961 ctttagaacc gactagatct gtaactttgg gctttccttc tgtaatcgtc ccagttttat
    10021 ccaaaacaat gctgtctaag tgagcagctg cttctaatgc ttctccgcct ttaattaaaa
    10081 ttccatttcg tgcaccaaca cccgttccta ccataatagc tgttggagtc gctaaaccta
    10141 atgcacatgg acacgcaatg actaatacag agacactatg gatcaatgcg agttgccaat
    10201 caccagtgat caaacctgta gctatgagcg tgataaacgc gatgactaat acgatcggaa
    10261 caaaaatccc tgagatccga tctgcgattt tttggattgg ggctttgctc ccttgagcat
    10321 cttccaccat ttgaatgatt tgcgctaata ctgtctgctt gccgacttgt gtgacttcca
    10381 tgtgtagcag accattcgta ttgattgtcc cgccaaaaag cgtgtcatcc ggattctttt
    10441 ctacaggaag attttcacca gtaagcatac tttcatctat cgcactgctt cctgaaataa
    10501 ttcgaccatc tgctggaatc tgctcaccag gacggatgac tagttgatca ccaacgacta
    10561 cttcttcaat cgctaacgtt tcttctttcc cgtttcttat cacttgggcg gtttttgttt
    10621 gaagagacat caattgtttg attgcattac ctgttttcgt ttttgctgta tgttccaaat
    10681 atttgccgag caaaatcagc gtgatgatca tactgctgct ttcaaaataa agatcttggg
    10741 ggtgtccacg gaaaaatccg ttgtaaatgc tcaatgcaaa ggcagccgat gttccaattg
    10801 ctaccaatac atccatattt ggtgctttcg acttgattgc atggtacgca cctttataaa
    10861 aacgagcacc gacataaaat tggacaggag cagacagcac taactggacg attggaaaat
    10921 gaaaaaagtg aacaatcgcc gcatggcttc ctaacatcat agcaatcatt gacaacatca
    10981 acggcaaagt caaaaccgta ctcaatataa gatcgaatag catttttttc aaatacgctt
    11041 gcttttcttc tgcaattttt tgtttgtggg cttcatcgta caaaattgca ccatagccga
    11101 tgttttctac gctttggatt aatttttcag tggtcgtccc ttcatatttg acacttgctt
    11161 tttcagtggc caagttgact gtggcattga ccacaccagg ttgtccgttc agctcttttt
    11221 cgatcctagc agaacaattt gcacaagtca tacctgtaat gacgaacgta tccattttgg
    11281 tttctgctgt tgccatcaaa tcacctctgc tttataaccc aatccattga ttgcttgaca
    11341 aatcttttcg gattgaactt ttgtttcatc aaacttgacc gtaccgtttg cttttttcaa
    11401 atttactttc actttttgca cgccgtcaag tgcgcttact gtttcttcta ctcttgctgc
    11461 acaatgattg cagctcatac cttcgattga aaattgttgt ttcatttttt cttcctccta
    11521 ttgatgttgt ttacattcac attgacctgg gatacagtta cattcgatcg tatctactgg
    11581 ttgcttttgt tccagttgtt tcaaaatctc attgatatct tcttgagtca aagtggcttc
    11641 ttctactaga tccgcaatcg tctctccaat cctttttgca cagatatggg aaaaaagatt
    11701 ttccgtcgca ctgcgaactg tttccatctc cgagacagct ggatgataaa tgaatttttt
    11761 tccttcttgt tctgtccaga tcacttcttt ttttaccaaa cgacccagca aagttttgat
    11821 cgttgctggt ttccaatcca tcgaatcagc taagatctgc gtgatttctt gggctgtcgt
    11881 ctgtccaagt gtccaaataa cacgcatgat ctcccactct gaatcactaa tctttactgg
    11941 cgttttgact ttttccattt atttcacacc tttcgtttac aaatgtaatc attaacttat
    12001 gaatacagat tacacttgta aacaaaaact gtcaattatt tttgctctct attttcaatt
    12061 tagttaaaaa ttcttgtttc tctcgtcttg ttttaaggat gatcagttta tttcttgcat
    12121 tagctacttg gatcagctgc tgtatttttg gttcattgtt tccttcaaaa tgccagataa
    12181 aagataaaaa ttctaagtat ttcctgttta gtttctccga aaatgattca ggcatatctg
    12241 gccgagattt tttgtttatc tttcttttta gagaacgcca aactactcgg tagatggcga
    12301 gataacgcgg cactttaagc caaatgatct tatctgcttc tttaatcctt tcctctaacg
    12361 tagaagtata atttccttca acgatccatg acggattaga taccataaac gattgctgtt
    12421 cttgcaaaaa ccactttttt gcctgaatgc ggaaagccgg tgatttcttt gatcttcctt
    12481 gcgagggacg attttcccga cccactttgt ccaatcacga tgatcttcat tcttctccct
    12541 cacttctctt tggaatttgg ttcgatcata ccacttataa cgccgcaaaa aaacaaggga
    12601 ctgtgacaaa agtcgtgtca aagtcccttg tttcgaataa atggtgttca atcagctgac
    12661 cttcagcatt agctcaaaaa acggcaaaat atgaggagct atttttgttt ggctgttctt
    12721 attacttgat gcaggacagc tttttcacaa cctctgattc acggtgttcc agaagtagct
    12781 tctgtcacaa cctcttgtaa caagtatttc tattttaagg ctgaaccact gtttctggtt
    12841 cagtaatttc tttgtttttg tctcgacgag caattgctgg caagatcata cctaataaga
    12901 tcaagacaat tggtgtgatg atattcgtga ataattggaa ccaccatgct ttaggatcag
    12961 cagtataatc tacttttggc accattccta ggatacatgc aaacgcagtg aaggcaaagc
    13021 accaagcacc aaagataaaa ccgatttttg gatttttaac aaatttatat tcagaattga
    13081 atttcttata agctttattc aacatcatat atgccaagaa cacccatagg taacgcattg
    13141 gcatgacaac tgagttcaag ttcgtcaacc attttactaa ttcattcata tctttgatac
    13201 caaagatcgg caacaaaata atgattccta ctagtatacc tgtcagaaga taaccattca
    13261 ccaatgtacc tttacgatta cgtttacgta gccagtttgg tacaaattct ggatccgcat
    13321 ctgctaacaa gatttttagt ggtgcatcaa ttgaaaaggc taatgcggag atttgtccaa
    13381 tcatgtttgt caatgcgtaa atgatcatca atgagttacc gataccatag taattaccta
    13441 aaatttgaaa agcactgtat gccccatttg ccatcaagtc tttagggata ttatcgctag
    13501 caaacagcat acccattgcg actgagccta atacagcaca aatccctacc attgctgcta
    13561 agaagatcat ccctttagga aattcctttg caggatttct tgtagagttg acataagggg
    13621 atattttttc cgcccctcca actgcaaata ctagcatcga aacggttgtg aaataactga
    13681 aatcaaatgt tggaatatat gttttcactt gattcatatg tggtgtggca aattccatcg
    13741 ttggattgat gaacggtgcc gttacagcta aaagaataaa taagatcgac atgacgaaca
    13801 tcgctgttcc ggccagtccg ccaatcactt tcaaagtaga aagtcctttt gttgataaat
    13861 ataaaaaggc aagaaatata ataagtgaaa taactgctac catttgtact gacatcgtat
    13921 ttacgatatt tccatttcct tggactgccc aaccaccagc aatcagaatt gcttgaggct
    13981 tttgtgccaa ataaggaata tggacgaccc agtaagtcca agcggcatag tatgccagtc
    14041 gtttattaga tgtcgtattt tcgacccatg aactgacgcc tcctttacta tccttgaagg
    14101 ctgatcctaa ttgtccaacg atcaatgcgt atggaataaa ataaatggcc aaaatcaata
    14161 gccaagaggt gacaactgat actccttgct gagcatagtt attaactaca ttccccatgc
    14221 cccaaaccat gttaaatgca attaacgcaa cagtaaacca gcgtaattgt ttgttgttca
    14281 ttcgttctct cctttatgat aaaaatttgt tatccagatg taatcatatt caatttttaa
    14341 caaacttaca agacagtgtt attttatgtg tgtattcttt tatttaatac acatcgtttt
    14401 ttcatatttt ttccatcaaa aaaataagtt attttctttt atttcattga ccttattgaa
    14461 tttcaagcta ttttgaattt tctgcaaaat taaaaacatt agttatttta taatcctatc
    14521 tattttcttt acatttttca ttaagaaaag gatataaaaa aaagaggacg ttcattcgtc
    14581 ctcttcattg atcaacgttt attttttaca tgcaagcttc gcatggcatt caaaacagct
    14641 aatactgtca ccccaacatc tgcaaatact gcatctccca ttgatgcaaa accaaaagca
    14701 cctaaggaaa gtaccaaaat ttttatccca atagcaaaga caatgttctg tttgacgatt
    14761 ttcaatgttt tctttgctaa atggattgct tctgcaatct tcgatggttg atcattcatg
    14821 atgactacat ctgctgcttc aattgcagca tcgcttccca atccacccat tgcgattcct
    14881 acatctgctc ttgctagcac cggtgcatcg ttgattccat ctccgacaaa tgcggtcttt
    14941 ttattgttac tttgtaagat ttcctctaac cgctgcactt tatcttctgg aagcaattcg
    15001 ctatatatct tgtccacgcc tacttgcttc ccgatatgat cagcaatttt tttcgagtcg
    15061 cccgtcaaca tgactgtgtt tttcacacca gcctgtttta attgaaccaa tgcgtctacg
    15121 gcatcttctt tcaaggtatc tgcaataaca agataaccac tgtaactttg atcgatcgct
    15181 agataaagca atgtaccgat ttcttgactt gaaggaaaag agatttcaaa tctttccatc
    15241 aatttgtgat tacctactaa tacttgtttg ccatcaactt cagctaagat cccttcacct
    15301 gcaaattcct ggatattagc tgttggtaaa agcggacctg cgtagccttc taaaacagat
    15361 tgagcaatcg gatgtgtaga aaactgttct gcacttgcaa catattgcat aaatttcttt
    15421 ggatcaattc ctgttggatc gatcgtctgg atcgaaaagt cacctttcgt taatgtgcca
    15481 gttttatcga ataccactgt ctcagtttgt gcaagcaact ccaagtaatt tgaacctttt
    15541 actaatacac cgattttact tgctcctccg ataccgccaa agaaactaag tggcacggaa
    15601 atcactaatg cacatggaca tgagatcact aaaaatgtca atgctcgata gatccattca
    15661 gaaaacgccg ctctagaaac aacaagtgga ggtaaaacag ctaataaaac ggccaatcct
    15721 acaacgactg gtgtgtaata gcgcgcgaat ttagtaatga aattttcagc tggtgctttt
    15781 ttactgctgg cattttctac taattctaat aatttgctga tagtagaatt ttcataagat
    15841 ttttctactt gaactaaaaa tggtttattt ttattgataa atccgcttaa tactgtttca
    15901 ccaacatata cagatttagg gactgattct cctgtaagtg ctgacgtatc gactaatgat
    15961 tctccttcga tgatcgttcc atctaacggt acacgttcac caggcttcac aagtacaaac
    16021 tgaccgatca gcacgtcttc agggttcacg gtcatcaatc cttccgccgt ctgcacgtga
    16081 gctgtttctg gacggatcgc catcaattcg cgaatcgatt ttcttgaatg actgactgcc
    16141 aatccttgga aatactcgcc gatttgataa aatagcatca ctgctacggc ttctggatat
    16201 tcaccgatca acatagcgcc aatcgtagca acagacataa gaaaattctc atcaaagatt
    16261 tttcctttga cgatattcat cactgccttc ttcactacat catatcctat cagcagataa
    16321 atcaataaat accctatcaa gcgaattggt tcattaaaat caaataatag caataaagcg
    16381 atacctgcaa gggaaaataa aatccggaac agcgtatttc tctttttttc atttccatgt
    16441 tcagatactt tttccttatc tatttctgtt actttgacat ctggttctaa ttccttcact
    16501 actttttttg tttcttgttc aatcgtagcc aaatgatctt cctctgcgtc aatcgttagt
    16561 tttccagaag tgaagtttac cgttgcttcc ttgactccat tgattttctg aactcctttt
    16621 tcgattttca ttgcacagtt cgcacaatct aaaccttcta atctgtaact tttcatttta
    16681 atgacctcct cgcctcaaca tatgaatatc tcttcataac taattataaa tgaatggtta
    16741 ttcatatgtc aaggtatccg aaagaaaaaa tgaaaaacat ctgcatcgga aaatttcttc
    16801 catacagatg ttttggctta ttctttcttt ttctcttcga tcattgccac tgctcttatc
    16861 caacccgcac ttgccttctt gatcttgaca ggatagcaac taagtttgaa gcctgttgct
    16921 ggcaatttgt ctaaattcat taatttttcg ataggacaat atcttttttc aatccctgca
    16981 aaatgtcctt cccagatata cgaagctcca ctacgctttc aaaaatatag aaagagttct
    17041 ttaatgaaaa ttatttcaac tttccataaa cagtaacccc atataaataa aagacgtaaa
    17101 agtcacatag aagaacttat acaggaaaac gcatagagtc aggtgaaata catgtatccc
    17161 acctggctct atgctttatc taacctttat tattctcttt tatacaattt ctgcaccgaa
    17221 tggcatcaac gccaatcgag caagtttgaa agattgcttg gcaaaaggta tccctatgat
    17281 cgtgatacat agaaggatgc cgctgacgac atgtgcgaga gcaattggta ggcctgaaac
    17341 gatcagccag atgatattaa cgatcaatga catgccagac tggtgataaa ttacttcttt
    17401 gccaaaaggc gcaaaagaaa gggaggcgat cttaaaacat tgcataccga ttgggatacc
    17461 tatgatcgtg atacaccaaa gtatccccgc tgctaaccag ctaagaccac cgattagtcc
    17521 gccgaatata aaccagatga catttcctaa acaactcatt gtatttctcc ttctattact
    17581 gtttttgatt aaaactgggc aaaaattgat tttattcttc tttttttagc ttccagcatc
    17641 aagtattctg caaatttttt ttggatttct tccgtatttt cttttgttac taatgtgtgt
    17701 tggattagtt ttcctgatgg tcgctgatcc aatcgttctt ctgactgttg cacgtccgct
    17761 tgcgcaaatc cgataatccg tttttctttt gttacagcaa cgagcaatag tttattctta
    17821 tcatttaggt cagctgccgt ttttgctttt acgaatgatt ctggatgatc agtaatcagc
    17881 tgattgattt gaggtaaatc ttcttttact gcccacctta tttccatcat ttttcacttt
    17941 tctttcgctc atttttatct ccactaaaaa aatcgactgc gacaattgca tctatccagc
    18001 tttgtgcaat gatctcatgt ccgccaatcg ttgggtggac cccatcttct cttgtatatt
    18061 tcgaaaaacc ataagcaatc cctaaagcat tgaatatacc atctaaagga accagcaatg
    18121 cgccatattc cttagcaagt ttacgaacaa tttggatttt agggtctaag tctttacgcc
    18181 agccatgacg gtcgtcggtt ataggaagta caaacggctc catcaagatg atgttgcaga
    18241 tttccgctgt ttgaagttca tttagtaatt ttcgataaac ggattcaaat tgctgagcag
    18301 atttctcagt tccgaatata gcctgatcgc tagtattatg ccaggtatca ttgattccga
    18361 tcaaaatcga tacatagtca ggttgtaaag ctaaacaatc ttcttgccag cgtacttgca
    18421 gatctgcaat tttgtctcct cctatcccac ggttcatgca ttcaaattcc atcgttggat
    18481 attgggagat tagttttccg gcaatcaaaa gtggataacc tttacctaga tcagttgctt
    18541 ctgctcggta tcttcctgcg tctgtaatgc tatcaccgat aaataatata cgttttttca
    18601 tttgtcaaaa actccttttt gaataaaacg atgattttct tccttatatt atcccataag
    18661 ccctatcaag acgaaagaga aaatatatgt ataaaaaatg acaccagcct agttccttca
    18721 aacaattgaa tgaacgctca tcaagtgtct agttttttat gctatttttt tcttaacgtg
    18781 ccggattccc cttgattcct tcgttgtttg gtccagagag atcgatcaca cccaagcttc
    18841 cgtcaatcag accactgatg acgccatcga aacatatatt tataggctca tctaagactt
    18901 ctagtaaatg tccctttact aatatctctt ctagattgct tgggatagat tggtcataac
    18961 tttatctggt ccgatgattt caagggtgaa ttgaacttac atatggatta ttgcatgctg
    19021 aaaatacgga cctactccat cttcgaatgt tactattagt ttgttttcag gtgctagatg
    19081 ctgctttatt tcaacagcag aagcatcatc aaatcagatt tccatttgaa ttaccttctt
    19141 tgtaaatgtt tgattgattg cgatttcgtt atagaacctt gtgaatcaaa tagcaatttc
    19201 ttttgctttc ttctcttgtc tgctggaaac ttccattatc ccgtatttaa aaaaaagaac
    19261 tgcgagagtt cgcagttctt ttttttaaat aaacgttgct ctccggccac aagtcacaat
    19321 agtcatcaaa catgaaaaac tgttttttga aattactcct tgtgactcgg agctgaacgg
    19381 caggcgcaca acctcttatg gataaattcg gttcaacaaa cgtgggaatg gaatagcttc
    19441 acgtacatgg tcgataccag caatccaagt gactgttcgt tcaagaccta gaccaaaacc
    19501 agagtgcgga acagaaccgt atttacgtag atcaagatac catgaatatt ctttttcatc
    19561 caaaccgtga ttacggattt gttctagcaa gaaatcatag tccgttgccc gttcgcttcc
    19621 gccaataatt tcaccgtaac cttctggtgc gatcatatct gcacaaatta ccacgtcttc
    19681 tcttgttgga tgtggtttca tgtagaatgg cttgatggct tttggatagt ttaggataaa
    19741 gacaggctgt tcgaatgaat tcgcaatgaa cgtttcatga ggtgaaccaa agtcttcgcc
    19801 ccattcgatg tcatcaaatc catttttctt cagtaattcg attgcatcat cgtaagtaat
    19861 acgagggaat ggcaattttg tataagtttt taagacttct ttgtctcgtc ctagtacatc
    19921 taatgcgtat tcgcaatgat ctaaaacatt ttgtactaag aatgctacat attgttcctg
    19981 tacttctaaa ctttcttctt gatgagtgaa agccatttct ggttcgatca tccagaattc
    20041 gatcaaatga cgtcgtgttt ttgatttttc agcacggaaa gtcggaccga atgaaaagac
    20101 ttttccgaaa gccatagcag cagcttccat gtataattgg ccactttgag ataaataagc
    20161 tttttgatca aagtaatttg tttcgaatag atccgtagtg ccttctggtg cagaaccagt
    20221 caagatcggc ggatcaattt tgacaaaatg atgttgatta aagaattcat acgttgcacg
    20281 aatgatttca tttctgatct gcataatcgc atgttgtctt gaagaacgca gccataaatg
    20341 acgatgatcc atcaagaagt ctgttccgtg ttcttttggt gtgatcggat aatcgtggct
    20401 ctcgcttacc acttccacat ttgtaattcc gatttcataa ccaaattttg aacgactgtc
    20461 ttcacggatt tctccagtta ctaacacgga tgtttcttgg tgtagatttt tagctgtttg
    20521 gaaaacttct tccgacactt cgctttttac aactacacct tggaaaaagg ctgttccgtc
    20581 tcttaattgt aaaaatgtga tttttccgct tgatcgctta tttgcgaccc aagcgccgat
    20641 tgttactgtt tctcccacat ggtttttcga atcgatgatt tgaatttgtt ccacgtatga
    20701 ctctcctttt atattccttg aatttcttca ggattaattt tgacttttac tctcaataaa
    20761 ttgcttgatt ctgcgtaccg cttcttctaa cgtagcgcga tcagtagcat aactcatgcg
    20821 gacattttct ggtgccccaa agccttcgcc agtcacaagc gctacccctg tttcttctaa
    20881 aagatcttct acccattgtg tgacattttc atatttacac atgcgtagcg tttcttttac
    20941 attaggaaaa agatagaaag ctccttgcgg cttttcaaga gaaacacctg gcagttctgc
    21001 cacaagcgga tataacgtat tcaggcgttc ttcaaaggcg ttccgcattt cttctactgt
    21061 atcttgctca cctttcaatg cttcaacagc tgcatattga ctgactgctg tcggattact
    21121 ggttgactgg gaagcaacag caatcattcc attgatgatc tcagaattcc ccaccgcata
    21181 accaatgcgc cagcctgtca tcgcatatgt tttagacacg ccattgatga tgatggtctg
    21241 atttttgatt gcttctgaaa tcgttgcaat tggagtaaac tcattgccat tataaactaa
    21301 acgaccataa atatcatcag caactatcaa aatatcattt tttactgccc attcaccgat
    21361 tgcctgaagt tcattcttgc tataaatcat tccagtcgga ttcgatggag aattgatgat
    21421 tacggctttt gttttttctg aacgtgcttg ttctagctgt tcaacagtaa ctttccagct
    21481 attggattct tcccctttca caaagacagg aagtccttct gccagtttta cttgctcacc
    21541 gtaactgacc cagtaaggca caggaatgat gacttcatct tgcgggtcaa ggatcgtttg
    21601 gaaaagggta tacaaagcaa atttcgctcc gtctgttacg actgtttgtg aaggttcata
    21661 gacaagtcca tagtttttct tcagataatc aacgattgcc tgtcgaagct ctgggattcc
    21721 tgctgttggt gtataatagc tagctttccc attgcggatt gcttcgattg ctgcttcttg
    21781 gatgttttct ggtgtcgcaa aatccggttc tcccacagtc aaactcagca catctctgcc
    21841 ttttgctttt aatgccttgg cctttgccgc agctgccaaa gtgacagacg gttctaatcg
    21901 ttttgttctt tttgataact ccatcccatt cattccttta ccttttaaat tcccttgatt
    21961 gtcttgactt catctccaga ttcaaagcca atcaggtaat aattcagttt cccatttgtt
    22021 tcttttccta ctacttccca cacaggctga tcatcataca tccctaaagc agctttttcg
    22081 atttggattt caggatgggc cgcagcaatt gtctgttttg cttcgttttc agtcagtccc
    22141 tttgtttgat ccaatacttt tactttttca ccagatttag gaatgataac ggcgatttct
    22201 tttcctttat catttgtacc tgtcagtgaa aagtatgtct tctttcgagt aaaccaataa
    22261 aaatgatcgg tttcctcaag atttgcatat ttctttgcga tttctactgc ttctttcttc
    22321 gcctgcaccg ccggacgact ggaacgcaag taaaaaatcg agctgatcac gatgaccgct
    22381 aataaaaata cgatcaagcc aagtaacacg cgattaatcg ttttttcctt tgtctgtttt
    22441 ttcttcaagg gtcgtcgctc ctttatcttc attccttttc attatagcag attttaatta
    22501 tgaaaaaaga cagctcctct aacttccttc ttcatttttc tttaagaatt catggatatc
    22561 ctgatagatt tcctccatag ctagctcttt gatcggtact tttttcggta atgcatggat
    22621 gatccgtgcg ccatattttg cagtgactag tcttcgatcc aaaataagca atacgccacg
    22681 atctgtctcc gtccttatca agcgacctaa tgcttggcgt aacttcaaag ctgctttagg
    22741 tacggattcc tggtaaaaag gattgatccc ctctgattct aaataagcgt tcctagcttt
    22801 gaccatcggg cgaagcggat tatcgaaggg taaacgtgtg actacaacga tctgcaaagc
    22861 atctccaggc aaatctacac cttcccaaaa gctgtcagcg ccaaacaata cgcttttttc
    22921 tgacaaaatg aagcgtttca aaagcttctc ccggcttcca ccaaaccctt gagctagtat
    22981 ttctcgtcct ttttctagca tagacagatg cattttctga tagaccttct gcaaaacatc
    23041 atgagaagtg aataacacaa gtattggccg atcttcttgt tcgatcagac ctgtaagtgc
    23101 tgtctctaaa taatcgatgt atgcttgatt gctgacttga tggacggcag gtgcctcttt
    23161 tggcacatag atccttgcct gtttctcata atcataagga gaggcaatca ctttaagagg
    23221 tgctgcttca atgccaagcc tcttagagag atagtgtcga ttagggccga tcttcagcgt
    23281 tcctccaata taaagaatac gatcatatcg aggataccaa acagtcgacg gcaacatagc
    23341 cgcttggaaa tcatggagat gaaaaatcat ctgctgcgct gtgttcccag gaacgaacca
    23401 atggatcaaa tgatctcgcc agtcttccag ccattgatcc atcagcaagt actgcttgtc
    23461 taattgatca aataagctat ataaacttgc taaatcgatc cgcatctggt ttgtccagct
    23521 ttcttggata atctcaaaag tcagacgcat ttgtgactgt agctgctgaa tctcctgata
    23581 aaaaagctgc aggtgatcaa tggctgtccc agattccagt ggcagctgat ccagcatctc
    23641 ttttgtcatc atgacttctt ctggatactg tccgttgggt tgcttgccgc tcactaaact
    23701 cattaatcct tctgccatac aaatctgtgc ttcgcatagc gcagaaagtt cttctcgata
    23761 aatcgataac cagcgtaagg cttcatgttc tttcggtaaa tagctttgta ttcggtcaaa
    23821 cagctgattt ggttcttcta attgctggat ttgtttatgg aaatagttaa tacttaatcg
    23881 tttgtcattt actttttcag cgatttccgg caaatgatgt acttcatcga tcaacaaaaa
    23941 tggactagct ggaagcaatg gttcttttcg ctgtgtttct tgtactaaaa acgcatgatt
    24001 gacaatcaaa aaattacttt gcctgatttt cttttgcaag aaacgaacaa agtcttgttc
    24061 gtaaaacgac tgatttcttg ctaaaaaccc agtacctctg tgcgcgatat ctgcaaagaa
    24121 tgcatggttc aagtttgtta aatgtaattc atccaaatca ccggtcttcg tttgcgtcag
    24181 ccaaactaag accgccattt gatacaaagc atattgtttt tgttctggtg gttccacaag
    24241 tgttccttta aatcgttgca ggtcgatata gtggcgataa cttttgataa ttacaggttg
    24301 tatgggctga tctagcaatt gattcagcaa tggaatttct ttttctatga tctgctgctg
    24361 caaaactagc gaaacggtac tgatgacagc gggtttttca ggtgtagcca aataactcaa
    24421 tggcaacaga taaccgattg ttttacccat tcctgtagct gcttcgatca tcaaatcttt
    24481 tgattcatct tttgtgaaat ggtcgtagat cgcattcatc agtctgttct gttcttttcg
    24541 atacatcagt gttttaccaa acatcttttc ttttgcttgt ttttttcttg gatatgtccg
    24601 ctcgccgtaa tggacggatg tgaatagttc cacttctttt ttctgcaaag ctagcccatc
    24661 gacgatttct aatgaggaat caagtggctc tggtttttct ttcatttctt caatcacatg
    24721 ataaatgaaa cgactcgtat ccatacctgt gtgtctactt aatcgcgcaa tctgctctaa
    24781 cgtgatgatt ggcaattttc tcatccgttc ttcgatcagc aaaagaagtt gacctgtcac
    24841 ttcggcatca ctatctgcct gatgaggatt ctcatgaata aatccaaggc tttctgacaa
    24901 atcagctaag cgaaagcttg gttccgtcgg caagaaaatt tgtgcaagtt ctactgtatc
    24961 gatgcctggt atcttcaaac taggaagtcc gcatcgcatg agttcatggt tcaaaaaatt
    25021 ataatcaaaa taaatattat gcgcgacaaa tatggtgtct gccaaaagat tgtaaatcgt
    25081 ttgggcgaca tcttcgaaat aaggcgcttt ttggacgcgt tgattcgtga tccctgttaa
    25141 atgctggatt tgttttgaaa tcttccgccc tggatgaata tcgatcgaaa aacgtgaagt
    25201 gatccgacct gattcaatca tgacgcagcc aaattggatg atccgatctt cttttggatt
    25261 cgtcccagtc gtttcgatat caacgactgc ataagttctt ggtttcaaaa tatcttcctc
    25321 ttctcctgcc tctaatgttc ttttgaaatt atactacaaa gtgcagaaaa atactctcat
    25381 cgctttttaa agaaaataga caaaaacgcc cagacacgca aatgtctggg cgtttatttt
    25441 ttagtatagg ttaattaagc tttagtaacg tttgttgctt gaggtccgcg ttggccttct
    25501 tcaacatcga aagttactgc ttggccttct tctaaagttt tgaatccgtc gccttggata
    25561 gctgagaaat gaacgaatac atcgtttccg ttttctgcag tgatgaatcc aaatcctttt
    25621 tctgagttaa accattttac tgtacctgtt tccataataa aaatcctcct tgtgtatcaa
    25681 cacatatttt gcaattactt gaatggtgta tatctggaag atgtttattt gaaacaagct
    25741 accacagatt accgaacaaa aactactcct atagtatatc ataccgttta caaaaaagct
    25801 agagtttttc gacttttttt attaaaaaaa gttttctcag cgattacata aagtgaaaaa
    25861 acgaactttt tttaaaataa acgacatatt gttcagttgg tgtttttttg ctctgatgtt
    25921 cttctttaaa ctaaaaaaat tattctttct gactacttat cggttctgtt tcaacggtcg
    25981 aggctgggga agattcttta gtaggtctga tgttctcatg gtatttctga tagcctatga
    26041 cagtgaccag aagaacaagg caaaccaata cgatcaacga gattagtttt tcttttttgc
    26101 tcatatctat acctcataaa taacaggatg atgtccatca tatttctgag tatgagtaaa
    26161 agcaaaagct gcagctgtag aatgtaagaa atgccagcaa ctttcataat cacaggtcgg
    26221 acagtaccaa aagtaaccaa atcttggctg caaagaccgt tcttctgttt caggaatcgc
    26281 agaatcaaga tctgttgtct gtacataaac atttttgtac agacgtgtgc ctgtaatagg
    26341 agaccgctgt gtttcttcag caaaaactgg ctgacttgtt aaacttaggg atagaaacag
    26401 caaacaccat aacagtcttt ttttcataaa gacctcctta tcgataagtt tttcccaatt
    26461 aaaatcatat ctcaagaacg cccgttattc acattttgtt cataaatgat gttttttgta
    26521 tcataatcgt caaaaagagg tcgtgaccac gtcacaacct cttttttaaa cggtgttcca
    26581 gaagtagctt cttcggaaat aagccgaaat ttcacaaaaa tttgagaaat aatttttgtc
    26641 aatctcttct tatttcttga agctgaccac ttctgtcaca acctcttcct taatactcag
    26701 gtcaaaacac tgatttcaca acctctttta ataacgaaga atcgctgtca gactcttttc
    26761 atctggcgca tctttttgat ccaaaataaa cactttcccg ccattaatga tcacatcatc
    26821 tgctaatgca ttcaacactt gacgccgatc atattctgtt tcaggatctt caccgaaacc
    26881 atcagctaag tttgacgtag cgataaataa atgtgacact tttccttctt tcgcagctgg
    26941 aacgatatca actaactgat cgatgaattt cttatctaag agtttattgt attcttttga
    27001 ttcttgagct gcgagttcac ttgacagctt cttcgcccct tcttcgatat ctctaaagga
    27061 tagctgcgca ggagaagcag aaatcgcaac gtctgcacaa taatagggat ttttagcaat
    27121 ttttttgaac atcgtctgat tttctggtaa tgcaaataaa taaatcggcc attgttcagg
    27181 attttcgaaa gaatccttta agtacgtatc aacagcttgg taatagttga cccaatcgat
    27241 ctctacttct tcatcttttg gatttacgcc atggaaagtt actccatctc gggctccgcc
    27301 tcctccatta gaagagaagt tcaaaccgcc acctgtcagt tcagatccca atgcagtaac
    27361 gacatcaacc ggtgcccctt ctggtagaga tacttctttt acttgtttgt tgctgacctc
    27421 atacaatttc atggagtcgc ggttcaatcc caataaataa taagaatagt tgaactgccc
    27481 atttttgata atagccagta aataaggaag atcacttaca tagtattgat cgtctaccgg
    27541 gatactcaaa cgatggatat aggtctggtc agcagttata atgaccgcta cactcgtagt
    27601 tgcactacgc caaaacgaag gatctcctaa caaattttca attttttctt ggaacggaga
    27661 ccagtcatgt tcggaatatt ttttctcaaa acgtttcttt gcttctttcg caaaattttt
    27721 gaatttgatc tgatcttttt ccacatcttg atgggcgaca tgcgtattca gcatgatcgt
    27781 gacgaatggt cctttagcgc cttctgaagt caattgtgtc aataattctt tatccttttt
    27841 tgccataaac gtacccctcc taaaaaaatc attcctttgt ttctatctaa agtctaccac
    27901 aacactattt ttccatacaa taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt
    27961 aaatttagct atttttttaa gttatcttca taatttcctc aattatttag ccccagtctt
    28021 caactattct tcatgaaatg ctgataaact aagaaaaaga ataaaagagg tggatcttat
    28081 gcatttaatt atgattttca gtttattaag tattatcagt ttatttgctt tcagcgcttc
    28141 ccgcttatta gatgcggaaa cacggcctat tcctgtaaaa gtacgtaaaa acgaccgcta
    28201 aacttccata acgcgcgttc ttatcctaaa gttttttcag aaacattgaa actctctc
//
LOCUS       DACEPD010000029        28011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.029, whole genome shotgun sequence.
ACCESSION   DACEPD010000029 DACEPD010000000
VERSION     DACEPD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.029"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(5..586)
                     /locus_tag="IW797_001203"
     CDS             complement(5..586)
                     /locus_tag="IW797_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1223989.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="IW797_001204"
     CDS             complement(769..1395)
                     /locus_tag="IW797_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1223990.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="IW797_001205"
     CDS             complement(1417..2748)
                     /locus_tag="IW797_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ1223991.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="IW797_001206"
     CDS             complement(2903..3304)
                     /locus_tag="IW797_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ1223992.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="IW797_001207"
     CDS             complement(3390..3596)
                     /locus_tag="IW797_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223993.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="IW797_001208"
     CDS             complement(3691..5193)
                     /locus_tag="IW797_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ1223994.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IW797_001209"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IW797_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ1223995.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="IW797_001210"
     CDS             complement(5764..6381)
                     /locus_tag="IW797_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1223996.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="IW797_001211"
     CDS             6602..7975
                     /locus_tag="IW797_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1223997.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="IW797_001212"
     CDS             8050..9162
                     /locus_tag="IW797_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ1223998.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="IW797_001213"
     CDS             complement(9234..10871)
                     /locus_tag="IW797_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ1223999.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="IW797_001214"
     CDS             complement(11039..11647)
                     /locus_tag="IW797_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1224000.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IW797_001215"
     CDS             complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IW797_001215"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ1224001.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13201..13953)
                     /locus_tag="IW797_001216"
     CDS             complement(13201..13953)
                     /locus_tag="IW797_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224002.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14094..15044)
                     /locus_tag="IW797_001217"
     CDS             complement(14094..15044)
                     /locus_tag="IW797_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1224003.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15045..15884)
                     /locus_tag="IW797_001218"
     CDS             complement(15045..15884)
                     /locus_tag="IW797_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1224004.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15881..16627)
                     /locus_tag="IW797_001219"
     CDS             complement(15881..16627)
                     /locus_tag="IW797_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224005.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16865..18235
                     /locus_tag="IW797_001220"
     CDS             16865..18235
                     /locus_tag="IW797_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1224006.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18290..19186)
                     /locus_tag="IW797_001221"
     CDS             complement(18290..19186)
                     /locus_tag="IW797_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1224007.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19467..20144)
                     /locus_tag="IW797_001222"
     CDS             complement(19467..20144)
                     /locus_tag="IW797_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1224008.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20235..21140)
                     /locus_tag="IW797_001223"
     CDS             complement(20235..21140)
                     /locus_tag="IW797_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1224009.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21315..22769
                     /locus_tag="IW797_001224"
     CDS             21315..22769
                     /locus_tag="IW797_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1224010.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            22785..23816
                     /locus_tag="IW797_001225"
     CDS             22785..23816
                     /locus_tag="IW797_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ1224011.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23823..25010
                     /locus_tag="IW797_001226"
     CDS             23823..25010
                     /locus_tag="IW797_001226"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ1224012.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25166..26548
                     /locus_tag="IW797_001227"
     CDS             25166..26548
                     /locus_tag="IW797_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1224013.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26520..26906
                     /locus_tag="IW797_001228"
     CDS             26520..26906
                     /locus_tag="IW797_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224014.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27106..27774)
                     /locus_tag="IW797_001229"
     CDS             complement(27106..27774)
                     /locus_tag="IW797_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ1224015.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         7798 a         5729 c         5149 g         9335 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggatag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgattc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacaaccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    11821 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    11881 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    11941 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    12001 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    12061 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    12121 tacatcaagc catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    12181 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    12241 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    12301 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    12361 ctttaatttt ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    12421 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    12481 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    12541 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    12601 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    12661 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    12721 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    12781 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    12841 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    12901 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    12961 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    13021 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    13081 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc
    13141 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    13201 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    13261 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    13321 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    13381 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    13441 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    13501 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    13561 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    13621 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    13681 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    13741 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    13801 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    13861 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    13921 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    13981 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    14041 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    14101 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    14161 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    14221 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    14281 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    14341 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    14401 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    14461 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    14521 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    14581 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    14641 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    14701 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    14761 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    14821 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    14881 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    14941 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    15001 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    15061 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    15121 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    15181 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    15241 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    15301 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    15361 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    15421 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    15481 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    15541 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    15601 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    15661 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    15721 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    15781 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    15841 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    15901 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    15961 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    16021 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    16081 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    16141 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    16201 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    16261 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    16321 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    16381 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg
    16441 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    16501 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag
    16561 ttttcaaaac agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac
    16621 gttccaaatc ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac
    16681 taaaatggaa agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca
    16741 ttttatgaaa attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga
    16801 aaaacatgtg ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt
    16861 ttgtatggaa gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc
    16921 actaggtgga acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac
    16981 agggccctca gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg
    17041 tgctttaggc gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag
    17101 cgagtacatc catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat
    17161 tgtcgtagga ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga
    17221 tttgccggca attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat
    17281 ttctgtcaag gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat
    17341 cattttgatg atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca
    17401 ggcagtaggg atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg
    17461 atttttcttt gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat
    17521 gactgctggt gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat
    17581 cggtcgaatc ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg
    17641 gaatcaattg agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac
    17701 atttgctgca ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc
    17761 agggattttc agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa
    17821 aaaatttctg aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg
    17881 tggaatctta attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga
    17941 aaatattttc gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt
    18001 gattttgatc agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc
    18061 ttttaaaatg ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt
    18121 attgattttc atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt
    18181 cttgctttac atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa
    18241 gaacgaagct tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt
    18301 ttagcgtctc accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga
    18361 tcgtttctac tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa
    18421 ctacggttgt cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg
    18481 tgtcttgtgt tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat
    18541 cagaagatgt tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca
    18601 aataaatatc ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca
    18661 aagtatctaa gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc
    18721 tgatcccttc gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata
    18781 aaccagctat tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag
    18841 gcgtcttttc ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca
    18901 aaccatttgc tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac
    18961 ttgtttgttt cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt
    19021 ctgacgaatt ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca
    19081 cggcaactgc tgccccaatc atgatcatcc gcttacgagc tgcctgtttt tgctgtcttc
    19141 ttcgggtcat ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca
    19201 atttttatgt ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc
    19261 gctgtgcaca tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca
    19321 agattgtccg tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag
    19381 tttctttatt tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc
    19441 taattgttgg atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta
    19501 aaggtcaagt cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt
    19561 ttttcagatt cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca
    19621 atccgctgag tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc
    19681 ttctcccaac gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga
    19741 taggtcatat ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta
    19801 tattcttctt ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt
    19861 cctagttctt tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt
    19921 tttaaatgag caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc
    19981 tgggccaaga gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc
    20041 aaatgatgcc aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa
    20101 gattcttcga tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt
    20161 tagtcttcgc tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat
    20221 tttaaccaga ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc
    20281 aaggtatcca aagcaatatt cttaaaattt tcgtttttca tacgtttacg atagttcgta
    20341 gccgtttctc cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct
    20401 acttgatagg caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga
    20461 ttgatccgta ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact
    20521 cgcgacaaat ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt
    20581 tcagcataat gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt
    20641 tgtacaccac cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt
    20701 aatgctgtta actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa
    20761 ataatgtcga tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa
    20821 agcgactgcc gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc
    20881 cacggatctt ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga
    20941 tcaccttttt tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc
    21001 acgacatgca ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt
    21061 ttagagaaac cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt
    21121 aaaaaaatcg attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa
    21181 gttaaataaa agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc
    21241 attaaaagat ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga
    21301 gaggaataca gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt
    21361 ttggacacga cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc
    21421 agatgttcgc aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct
    21481 tagttgtagg tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg
    21541 ataatactcg tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta
    21601 gttccgtact cttggtagca atctttacca atttctttgg cttagctact cataatacga
    21661 ctttatttat catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta
    21721 aggatatcgc tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa
    21781 aatttgctac gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg
    21841 ttgtccctat cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt
    21901 caggctggtt ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca
    21961 ctgccttggg cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga
    22021 tcaaagatgt gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt
    22081 atatcgctta tgcgattgcc aatgcagcaa caactgccgt attattctac ttcttcaaat
    22141 atgtacttgg ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt
    22201 tagtagctgt tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc
    22261 ttggagggat cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt
    22321 tgccaatcgt tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat
    22381 ccgctttgat gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta
    22441 acgaagctgt tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta
    22501 acgggatcgt agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg
    22561 atatcactgc tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt
    22621 tgatgatttt ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg
    22681 cacaaatcgt tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac
    22741 ttcacgatgc agtagttcct tcccattaag aaaggatctt gaaaatgaag attacagaaa
    22801 aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt
    22861 ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt
    22921 tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg
    22981 gtcgcaccgc agggagaatc gaaaatggcc acttttctct caatggaaaa acttaccaat
    23041 tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa
    23101 aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta
    23161 caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt
    23221 tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat
    23281 tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc
    23341 aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta
    23401 taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag
    23461 cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat
    23521 ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa
    23581 gaatccaagt agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg
    23641 gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg
    23701 agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag
    23761 ccggatcagt atttgaaaca gtgacagaat tcaaaatcaa aacaagaaag gaataactca
    23821 tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa
    23881 ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg
    23941 gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag
    24001 acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta
    24061 ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg
    24121 tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag
    24181 gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg
    24241 ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta
    24301 aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg
    24361 gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg
    24421 ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat
    24481 tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc
    24541 aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta
    24601 ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc
    24661 agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac
    24721 tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag
    24781 atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag
    24841 ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa
    24901 acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac
    24961 acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc
    25021 aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag
    25081 ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc
    25141 taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga
    25201 aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga
    25261 gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc
    25321 cgattcgaca gaaacacggg gtagcggatt aatcttggct tccagtcctg cagcaaaaaa
    25381 agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga
    25441 tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc
    25501 tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt
    25561 ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa
    25621 actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga
    25681 taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt
    25741 tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga
    25801 cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt
    25861 ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac
    25921 tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc
    25981 aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga
    26041 aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc
    26101 agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa
    26161 tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct
    26221 agcagatgct ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt
    26281 aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga
    26341 tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa
    26401 atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc
    26461 acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga
    26521 tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat
    26581 taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaaatgaag
    26641 aagaagcacg caaacagcct cttcctaaac aacaaatgac attgcaagaa atcgatagta
    26701 tactgacaga agccttttta tctcgtaaag cagtaatcat tcagctcaat ttaaaagatg
    26761 aatttggaag actcttggat gaggtcgaag ggcacttcat tggtgaagcc tatgaagatt
    26821 actttatgat tgaccatcag ccgatattgt gggaagacgt acggtatatt gagttaaagg
    26881 aaacaaaaaa atggtttgtg acatgagagg ttgtgacaga agtgatcaac ttcaaaaaat
    26941 aagaaaggat ttttcgaaaa ttgcttctta aatttttgtg gaattttggc ttatttttcg
    27001 aagaagcaac ttctggaaca acgtatatta gatatcagag gttgtgaaaa agtcgcttag
    27061 ttttgagtaa caagccaagg cgctgatttc acaacctctg tctatctatc catctattcg
    27121 gtaagattca tgcatgacgt cttctttatt tagtatgtag agcccttttt cttctgacca
    27181 ttcaacataa atcagctgtt ttacatctgc atagccttct ttcttcaagt tctccataag
    27241 ccatgcttca gtcaattgat tggactgaag cactttatgc tgaatttgcc cttcatctac
    27301 aagcaggatc gataaagctt tgtcttcatc tgattttttc aatacactca tttgaccggc
    27361 attctctagg ataacatgtt tggcattttc aagaaaaaag cattcttgct gtcttaagat
    27421 tgctcgcaac tgctccatct cgatatggtt atttgtcaac tctgtcatgt tgatgacgcc
    27481 gtctttgatt agaacagatg gctcgccttt gacccaacga ttgacctttt catttttttg
    27541 gacgatacgt tcgataccat aaatcattac tgcccaaagc gcaatggcaa ataggacatg
    27601 ccctacatgg acattgtcat cataggttga ttcttctaag atcccaccta aaacaagcgt
    27661 atagactaaa tcaaacggtg tgatttctga catggatttc ttcccaagca aacgtaccac
    27721 taataataaa ccgatcaacc cgaacaccag tttcattgta attgaaaaaa tcattttttc
    27781 tccccctaac tatctgttaa ttaaattata tatgctttct cacgaacacg catggaatat
    27841 acaaggaaga aatgtatcct tttactaaaa aaaggactgt gacactgtag tgacccccaa
    27901 aagttagact tttttggagt ggagaaatcc actccttttt tttatgctct aaaatgatat
    27961 ttttacacaa ccgttacggt tgttttacga aattggacga gactttgcca g
//
LOCUS       DACEPD010000030        27214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.030, whole genome shotgun sequence.
ACCESSION   DACEPD010000030 DACEPD010000000
VERSION     DACEPD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.030"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            82..3123
                     /locus_tag="IW797_001230"
     CDS             82..3123
                     /locus_tag="IW797_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ1224016.1"
                     /translation="MIHLVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDC
                     QKLLGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRF
                     KVAYFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKN
                     QYTGQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPF
                     NQGSNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDRE
                     VTKKTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIH
                     DQLNYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGR
                     KIIITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEY
                     YELEGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFE
                     PFHVYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLH
                     PWAVTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDL
                     DVLVAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGF
                     DEPLLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQ
                     TNLREEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKP
                     IMDRYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKE
                     TRENIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEII
                     HKINERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDK
                     VMEQYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
     gene            3313..3897
                     /locus_tag="IW797_001231"
     CDS             3313..3897
                     /locus_tag="IW797_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAQ1224017.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFISKKAI"
     gene            3894..5033
                     /locus_tag="IW797_001232"
     CDS             3894..5033
                     /locus_tag="IW797_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1224018.1"
                     /translation="MNELFRVHLEKAVNTLLKTELTAFLDYEKYDRIGFNTGNSRNGS
                     YDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETVIHLFRKGITMSEIAD
                     LIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKE
                     AIHIVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAG
                     AVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFA
                     EKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQ
                     NEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
     gene            5411..7453
                     /locus_tag="IW797_001233"
     CDS             5411..7453
                     /locus_tag="IW797_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1224019.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            7464..8084
                     /locus_tag="IW797_001234"
     CDS             7464..8084
                     /locus_tag="IW797_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ1224020.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            8096..8554
                     /locus_tag="IW797_001235"
     CDS             8096..8554
                     /locus_tag="IW797_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1224021.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            8573..8851
                     /locus_tag="IW797_001236"
     CDS             8573..8851
                     /locus_tag="IW797_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1224022.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            8876..10243
                     /locus_tag="IW797_001237"
     CDS             8876..10243
                     /locus_tag="IW797_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1224023.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            10258..11403
                     /gene="dgoD"
                     /locus_tag="IW797_001238"
     CDS             10258..11403
                     /gene="dgoD"
                     /locus_tag="IW797_001238"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAQ1224024.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            11430..11921
                     /locus_tag="IW797_001239"
     CDS             11430..11921
                     /locus_tag="IW797_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224025.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            12364..13563
                     /locus_tag="IW797_001240"
     CDS             12364..13563
                     /locus_tag="IW797_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1224026.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(14058..14816)
                     /locus_tag="IW797_001241"
     CDS             complement(14058..14816)
                     /locus_tag="IW797_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1224027.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            14984..15919
                     /gene="pfkB"
                     /locus_tag="IW797_001242"
     CDS             14984..15919
                     /gene="pfkB"
                     /locus_tag="IW797_001242"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ1224028.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            15916..17361
                     /locus_tag="IW797_001243"
     CDS             15916..17361
                     /locus_tag="IW797_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1224029.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            17389..17844
                     /locus_tag="IW797_001244"
     CDS             17389..17844
                     /locus_tag="IW797_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1224030.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            17863..18837
                     /gene="lacD"
                     /locus_tag="IW797_001245"
     CDS             17863..18837
                     /gene="lacD"
                     /locus_tag="IW797_001245"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1224031.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(18948..19509)
                     /locus_tag="IW797_001246"
                     /pseudo
     CDS             complement(18948..19509)
                     /locus_tag="IW797_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            19653..20267
                     /locus_tag="IW797_001247"
     CDS             19653..20267
                     /locus_tag="IW797_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1224032.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            20508..20891
                     /locus_tag="IW797_001248"
     CDS             20508..20891
                     /locus_tag="IW797_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1224033.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            21254..21763
                     /locus_tag="IW797_001249"
     CDS             21254..21763
                     /locus_tag="IW797_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ1224034.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            21863..22513
                     /locus_tag="IW797_001250"
     CDS             21863..22513
                     /locus_tag="IW797_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224035.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            22964..23902
                     /locus_tag="IW797_001251"
     CDS             22964..23902
                     /locus_tag="IW797_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1224036.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            23915..24967
                     /locus_tag="IW797_001252"
     CDS             23915..24967
                     /locus_tag="IW797_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1224037.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            25233..25868
                     /locus_tag="IW797_001253"
     CDS             25233..25868
                     /locus_tag="IW797_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1224038.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            26059..26850
                     /gene="msrA"
                     /locus_tag="IW797_001254"
     CDS             26059..26850
                     /gene="msrA"
                     /locus_tag="IW797_001254"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1224039.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT         8740 a         4392 c         5747 g         8335 t
ORIGIN      
        1 atagctgaat tgatggaact tgagagttat aaaaaagctc tcatctacga atacgtaacc
       61 ggtaaaaaag aagcctagga ggtgattcat ttggtagaga acaaagaaat tcgttttgag
      121 caggatattg agacttcact gactacgtat ggcaaatgga agaaagaaac attcaatgaa
      181 tcaaactttg atcccataat tggtttggat tgtcaaaaat tgctaggctt tgtccagaag
      241 actcaaccaa aacagtgggc acgtcaccaa cgaaactata acaatgatgg agaagtcaag
      301 ttccttcaac gctttaatca agaggtagaa acccacggca tgttacatgt attgcgcaat
      361 gggatcaaag accgcggtgt gcgctttaaa gtagcttact tcaaacctgc ttcagagctg
      421 aatgatgaaa cgatggaaaa gtatgaagca aatagtttta cctgtactcg ccagtttgct
      481 tacagtccta ataaccataa aaccattgat atggttttgt cgttaaatgg tattccgctc
      541 gttgctttag aattgaaaaa ccagtacact ggtcaaacag tagaaaatgc caaactccag
      601 tttcaaaaag atcgtgatcc aaaagaattg gtatttcgtt ttaatcgccg cttcttagtc
      661 tattttgcgg tggaccatac agatgtttat atgacgacaa agttggacgg taaaaaaacg
      721 tacttcttgc cattcaatca aggttccaat gggccgggaa gagttggtgg agcaggcaat
      781 ccgtccaata aagatggcta cccaactgcc tacttgtggg aaaaggtgct tactagagac
      841 aatttgatgg atattatcca acgttttatg aatctagaga ttaaaaaaga aaagcaaggc
      901 gacagagaag tcacaaagaa aacgttgatt tttcctcgtt atcatcagtt agatgtggtt
      961 cgaaaattgg ttgcagatgt aaaaagccat ggtagtggag aaaattattt gatccagcat
     1021 agtgctggtt ctggaaaatc taatagtatt gcatggctgg cataccattt agcctctatt
     1081 catgatcaac tgaactaccc aattttttca tcaattatta ttgtgactga tcgaacagtg
     1141 ttggatcgcc agttgcaaaa tactatcatg aactttgatc atcagacagg tttggtagaa
     1201 acgattgacg atcgcaaatc ctctcaagat ttaaaagaag ccattaatga tgggcgtaag
     1261 atcattatta ccactttgca aaagttccca gtgatctatc aggaagtaga tcaagtggaa
     1321 ggtcgtaatt ttgctgtgat tgtggatgag gcacattctt cacaaactgg gaacagtgcc
     1381 cagaagctaa aagccgctct ttcggacaaa tctaaatcct tggaagagta ttatgaactg
     1441 gaaggcgaag ttgacgatgt attaccagat gctgaagatg aactagttca acaattggta
     1501 gcacaaggta aacatcaaaa tttaagtttc tttgctttta ccgctacacc gaaagaaaag
     1561 acattggaaa tgtttggtac gaaacaacca gatgggcatt tcgaaccctt tcatgtttac
     1621 tcgatgcgcc aggcgattga ggaaggcttt atcttagatg tgctgaagaa ctatatgacc
     1681 tatagtactt cctacaaaat tgcccgtgaa attgaagata acccagaatt accaaagagt
     1741 gaagcaaccc gagcgattgt tcgtttccaa tcattgcatc cttgggcagt tactcaaaag
     1801 acggctgtga tggttgagca attccgagcg gttactcgta cagctattgg tgggcaggca
     1861 aaagcaatgg tcgttaccgc atcacgctta cacgctgttc gctatatgaa agaatttaaa
     1921 aaatatatag cgaacaaagg ttataatgac ttggatgtgt tggttgcttt ttctggttcg
     1981 gtagtagatg gagagactga atatacagaa acgaagatga atgtcacaaa atctggtgag
     2041 aatgttcggg agaatcaatt aaaggaagct ttccatacag ataattttaa tatcttgatt
     2101 gttgctgaaa aatatcaaac gggttttgat gaaccgttgc tgcataccat gtttgtcgat
     2161 aagaagttaa agggtgttaa agctgttcag actttatcac gactcaatcg aacaatgcct
     2221 gggaagaagg atacctttat tcttgatttt gtaaatgatg ttgaagatat tgaagctgct
     2281 ttccaaccct tttatgaaca aacaaatttg agagaagaaa tcaatgttaa cttgatttat
     2341 gatactcaaa gcaagctacg aaaatttaat gtttacaatc aaggtgatat tgaacgtgtc
     2401 atgaaattgg tgaaacaagc ccaaaagcaa cagaatgagc gcttgcttgg acgaatttct
     2461 agcatgttta agccgattat ggatcgctat gaggaaatga acaaggataa tcagtaccag
     2521 tttcgggtga ctctacgtaa ctttggcaag tggtatgatt atatttcaca acttgatcgg
     2581 ttattcgata tggaactatt ggaggaacga atttttactg gatacttgct gaagtttcta
     2641 cctaaagaaa cacgagaaaa tattgatatt tctgacaaag taaagttgga atattataag
     2701 ttacgtcagg acttttccgg tgaaatccat ctagtgaata aagaaggcga gctagtaaat
     2761 ccaaagtcaa ttgatgcagg aatcaaaccg ccggatgaac gagattcact agaagaaatc
     2821 attcataaaa tcaatgaacg atttccaaat gattttgacg aaggagaccg ggtgttagta
     2881 gagattttgt atcggtcatt tgccgataac ccagatttga aggttgtcag catggctaag
     2941 aataacgatg ctcagatgtt tgaaaaatcg ctgttccctg atgtatttaa agataaggtg
     3001 atggagcagt acgagaagaa caatgcagct tttgagaaaa tgtttgggca tgatgataag
     3061 tactttgatc tagtatatac attagttgct agagatttat acaagttatt acggacgaaa
     3121 taaaatttat aaataaggat tagacaatac aatttctctc aaaaataaag gagaagttgt
     3181 attgtttgct tttaagaaag ccctgaaaaa tttgttacgt actaaattgt ctgaactctg
     3241 tcatatcagc tatgtcagaa taaaggaaat agatttattt ttataaacag tgtcgtctcg
     3301 gagatttaaa ctatgtcaac gacaattgaa gtgaacaaac aaagcgtgaa gcagtttctt
     3361 gaaacaggga aaatcaaaaa attcgttatt ccagaatatc aacgtcctta tgcctggact
     3421 gacgagcaaa accaagtact atttgatgat ttagcggagt acacagcaaa caataacgag
     3481 agtacttact ttttaggaag cattgtggct tatgaaaatg atcataacga gcaagaaatc
     3541 attgatggac agcaaaggat aactacgcta tttttatttt tgagagctat ctatgctaaa
     3601 ttagaaaata gttgtgaaaa ggaagcttta ttcttgaaaa gccaaattga acccgcgtta
     3661 tgggaacaag atgatttaac aggtgaggtt aaaccagata aaattctgat tatgtctcga
     3721 gtaatggggg atgaggggaa tgaagaattt gctagtattc tagtaagtgg tgaagcagat
     3781 gttaaatcaa aaagtaatta ttcgaagaac tatattttaa ttcaacatct attaaatgag
     3841 tatgcaacaa acgaaccact atcattttat cgttttatca gtaaaaaggc gatttgaatg
     3901 aattattccg tgtccatttg gaaaaagctg tcaatacgct tctcaaaacg gagttaacgg
     3961 ctttcctcga ttacgaaaag tacgatcgca ttggttttaa cacgggtaat tctcgtaacg
     4021 gctcctatga ccgtacggtc aagaccgagt acggggaact tcatctccag attccgcgcg
     4081 accgcaacgg cgagttcaag caacagactg ttcctgctta tagacggacg aatgatacct
     4141 tagaggagac cgtcattcac ctcttccgaa aaggtattac catgtcggaa atcgcagact
     4201 tgattgagaa aatgtatggg catcactaca cgccccaaac catgtccaat atgactaaat
     4261 catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt tatgccgcta
     4321 tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag gaagccattc
     4381 atatcgtagt tggcattcgc ccggacggtt caaaggaagt gttgagctat gcgattgcac
     4441 caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa cgtggcgtga
     4501 aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc gtgcagcggt
     4561 tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat atcagtcaca
     4621 aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg tatcaagcgt
     4681 catctaaaaa ggcggcattg gaagcacgtg gtgcttttgc ggaaaaatgg aaaaccagtt
     4741 atccaaaaat ggtggaatcg atcctttcga acgattacct gctcactttc tatgatttcc
     4801 cattagccat acgcaagact atttactcta cgaacttgat tgaatccttt aataagcaaa
     4861 tcaagaaata cagccaccgc aaggaacagt tccaaaatga agagtcgatg gaacgtttct
     4921 tagtctcgtc ttttgatact tacaaccaaa aattcctagg tcgcagtcat aaaggtttcc
     4981 aacaggccga aggcgagctc gaacaaatgc tgagtcaacc gatggagaat tagaaacaat
     5041 ctacagtagg gaagaaccat ttacacaaaa ttattgacgc tcccaaaagc attgttttta
     5101 gcagtttaat attattcttt ttcaattaga ctgacttgat ttagttacgg aatagatact
     5161 ttaatgtgtt gaagaagcaa cgtcccgtgt ttatttacta aagcatccaa tattctaccg
     5221 aatatttaat attcacatta gccctaaaat aattgtcata ccatgttaca tttgatataa
     5281 cagcgttcat tttaaaaata gctgttagtg tgacaaaaca ttaaaccaag tgtgacaaaa
     5341 aacgtgatga aagctttccg gaaacgtatg tgaacaagta tataatgatt ttgtcaggag
     5401 gatagtcaaa atgaataaac gaattcgtga aatactcaaa ctgattatta aaaaccctga
     5461 aatgaaactt tccactttaa caagtgaact tgatttgaca cgtcgtcaaa tcaattacgc
     5521 gatcaatcaa tttaatgaag aattgaaaac aaagaacatt ccgttaattc agagaagtca
     5581 ttctggaaat attacggtac caatcgaggt tatccaaatg atatcacagc ctgattacga
     5641 cataggtgat gatcaggaac ttgtcgttct cactgatagt gaaagacaag cattgatagt
     5701 gatgactctg atcgctaata tcgattacat cagtctggat catttggtag atgcggttga
     5761 tgttagtaag acgactgtta tggaagatat caaacgaacg gaaccgttca tcagaaatta
     5821 tagtctgaca attcgatata atcggatgaa tggttaccaa ttggttggtt cggaacaccg
     5881 tatcttacag ctgttatcgg atctagtaaa acggtttccg atttttcgcc gagaaatgat
     5941 tcgcagaaaa ttggcacaag atgtttctga agaagaaatc gtccatttga tccataacat
     6001 ggaacaaatg ttgcatctta gttattctga tgagtcggta gactatttgc agacatcagc
     6061 gagattttta gtaagccgtg ggatcaagat gtcagataag cacgcgtttt ttcccgatga
     6121 tgtgacggat actcctgaat tccgaatgct gatgatcttg gtacatgaaa caggttggag
     6181 tctatccaaa agttatatgg agtggttcac gttattattt ttaacttcca atatttttga
     6241 gagcaaaact actcaaaatt atgattcgga tcaagaattg cgaaaattga tcagccaaat
     6301 ggttgaaaat ttccaaaatc aaacgttgat tttgatcgat gaccgagaag actttgaacg
     6361 taggatactg agtcatctgc gtccagcctg ttttcgaata cgctataact tgagtttagg
     6421 agtttattca atagatagtc tgatcaagga tagtaatcat gcgattttga ttgatttgat
     6481 gaaagaattg attattccta ttgagaattg gctaaataag gcctttccaa atgatgaatt
     6541 agatctttta tcctattact ttggctatca attaagtaat cataataaat tgaataagca
     6601 acgacaaagg gctgtagttg tttgtactaa tggtgtcatg gtctcaaagt tagtacgagc
     6661 caatatggaa aaactttttc cagagatcca ttttttagct tccctatctg tgcgtgattt
     6721 ctacaaattt gaagctgatt atgatctggt ctttactacg acgccactaa aaagttctat
     6781 gctgcagttt atgatcgagc caatcatgac atatcagcaa caaatcagtt tacgatatcg
     6841 agttttgagt gatttaggaa ttactaaggt tgatcgagca atggatgaat tgttaacaat
     6901 cattaggaaa tatgcaaata tcaccaatcc agtttcgttg cgagaagaac tgcagtactt
     6961 tttgatcaag gaagatggaa gcacgccgct ggacaacttc aatgtattgc cttcattgac
     7021 ttattacttg cggccgagtt atgtacaaat cgttgaagaa gagatgtctt gggaacgagc
     7081 agtggaggtg gcttgccgac cgttattgga acatcaaatc attgactcgc gttttatcgc
     7141 tgattgcttg gatcaaatca gacaaccggg atatgcaggt tacttgggaa tgaagacttg
     7201 cattccccat acaacggtcg ataaaggtgt aatcagtgac ggtgtcagca ttttagtttg
     7261 taaaaagccg atacgatttc ctaatggtga agatgtcagc ttgattttgc cgctttcatt
     7321 ttttgattta acgaagcatc tgcgagccat caatcaaatc gctgatatcg ccaaagatga
     7381 ttcattgatt gagcagttat tgattgctga tgacgaaaag acagtttatc aactattaag
     7441 acaaagatct taaggaggaa aacatgagac tagcagatta tcctaaattg acagtgatca
     7501 tgcgtggtta tacttttgat caggcagatg ctattttaca agcaatgcag gaatttgacc
     7561 atcaatttgc cgtagagatg acaatgaaca caaaaggagc tcttgaaaat atacatgaac
     7621 taaatcaacg ttatggtgac acgacttata tcggcgctgg gacggtaagg acattagaac
     7681 aagcccaagc ctgctatgag gctggtgcaa aatttttatt aggaccacat atgttcacaa
     7741 aagaaatgtt gtcttacgcc aataagaaag gattattggc ggtacctgcc gcaatgactc
     7801 cttcagaagt cgaccgtatg tttaaagaag gagctgatat tgtaaaagta tttcctgcag
     7861 cggttgttac tccacgtttt ttcaaggata ttcaagcacc tttaggtaag ttaccactaa
     7921 tgggggttgg tggaatcagt aaggagaatg cgaaagagtt ttttgaacaa ggtgctagct
     7981 atttaggtct tggttccggt atgttcaaca agcaagacat cgaagagcta aatgttaaaa
     8041 acttagcacg ttcgatgaaa gaattaattg aggctattaa ataggagtga ggactttgga
     8101 actttatttg aatgaggaac tcgtttttcg aaatgttcaa gcaaaaacag atagtgaagt
     8161 cttagcgttt ttagcaagcg agatgtacaa aaaaaactac gtgaaggagg aatatattca
     8221 agcaatccaa gaacgggaaa aagaataccc tactggatta ccatcaacac caccaggaat
     8281 cgcaattccc catgcaaact atgaaatggt caataaaaca actttagcaa ttgcgactct
     8341 taaagatccg gtcctatttc ataatatgga agataacaat gagcagttac caatccaaat
     8401 tgtgatcatg atggcgattg gggaaccaca tgggcaagtt gaaatgttac aaaaaattgt
     8461 tggaatcata caagatgagc cactgcggca agaaatgatc agagcaggca atgatactga
     8521 attattagaa ctattaaaga aagcggtttt ttaatataag gaggaaatca taatgaaacg
     8581 aattttagta gcatgcggaa atggtatcgc cacttcaaca gtagtagcaa caaaagttag
     8641 agagtatttg caacaacatg gcattgatgt ttcaacgact caaacaaaat tgatggaagt
     8701 tccaggaaaa gttcaagact atgatctatt ggtgacgact ggtcaatttg atggtcaaac
     8761 aggtggtgta ccagtaatca aagggatgcc gattttaaca ggtatcggtg ccgatcagac
     8821 aatggaagag attttgaact tactgaaata atagctgatc aaaggagctg aaaaaatgaa
     8881 aatcattatg aacgtcttta acttctttat tgatgctgga ccaacagtta tgttgccagt
     8941 catcatcacg attattggtt tgatttttgg gttaaagatt tctcgtgctt ttaaatcagg
     9001 tttgacatta ggtatcggtt ttgccgggat caagttgata ttggacttta tgacgaccaa
     9061 tgttggacca gctgccaagg cgatggttga tcgaactggt gttaaactag atgctttgga
     9121 tgttggctgg ggttcgattg cggctgttac ttgggcatcg ccaatcattc caatcttgat
     9181 ttttgccatt ctattggtca atatcgtttt gctgattttg aaacgtacac atacgctgga
     9241 tgttgatatc tggaactatc accatatggc aatcgttggt gttatagtgt attttgtgac
     9301 taagaatgtc ttcttaggtg taggtgcatc agtagtgatg gcaattgcga cttttaagat
     9361 ttcagactgg tcgcaaccga tggtagaaag tttcttcgga atccctggtg tttctttacc
     9421 aactgtttct gcgctttcat cactggttat tgcttggccg ttaaattggc tgctggatcg
     9481 tatcccgtta ttcagaaaat caaaattcac catcaaagat gcacaaaaat atttgggatt
     9541 ttttggtgat tctatgatca tggggttagt tattgggatg gtgattggtg ttttagccgg
     9601 ctatgatatc aaagcggttt tacaattagg tgtcagcatg tcagctgttt tggttttgat
     9661 tcctaagatg acttcattat ttatggaagg attgatgcca atttcagatg ctgcacaaaa
     9721 atggtcacaa aaaaaattca aaggtacacg tttatttatc ggattggatg ctgcagttgt
     9781 tgtaggtaat ccagatgtta tcacaacagc attgatcatt attccactaa cgattgcatt
     9841 agctttagta ttgccaggaa accgtgtgtt accattcgca gatttagcgg ttgttccttt
     9901 ccgcgtggca atggtcgttg ccttgactcg tggtaattta ttaaaaaata tcattattgg
     9961 cttagttgtg acagcttcat tgctatggtg tgggtcagct actgcgccaa tgctgacagc
    10021 aattgccaaa tcggttggta ttaacttagg aacttcttca atgttaatct catcatttgc
    10081 tgcgacttcc atgatccaaa gttatttggt atttatcgca tttgcttaca aaccaatcat
    10141 cggtattcca gtgttgttat tagcctttgc gggcgcatgg tattacttcg atggtatcaa
    10201 aaaagttaat catgaaatgg atgaaccatt gaaggcacaa tagtaggagg gtttaaattg
    10261 aaaatttcag atgtcacagt gtataaagta aaaccaagat ggatttttgt taagatctcg
    10321 actgacgaag gaatcgaagg ctggggagaa atgatctcag gaactaaaac agaaacggtt
    10381 gttgctggag cttatgagat aggaaaccgg ctgattggac gtaatccgtt tgaaatcgag
    10441 cgcctcttcc aagaaatgca tcgatctttc ttccgaggcg gtcctattaa tggaaccatc
    10501 gtttcgggtt tagaaatggc tctgtgggat atcaaaggaa aagcgtttaa tgttccggtt
    10561 tatgaattat tgggcggtgc agcacgtgat aaaatcaagg tttattcttg gattggtgga
    10621 gatcgaccaa gtgaagttgc agaacaagca caagatcggt ttgatcgggg ctttacggcc
    10681 gtcaagatga acgcgacaga agagctgcat tatatcgatt cctaccaaaa agttgatgaa
    10741 gtggtggaac gagttgcgtc tattcgcgaa cgttttggtg atgcaatgga tattggtgtt
    10801 gattttcatg gacgggtcca taaaccgatg gcaaaagtgc tagcaaaagc tttggagcca
    10861 ttccatccaa tgtttttgga agaagtcgtt ttacctgaaa atgaagaaca ctttaaagaa
    10921 gtagctgatt ctgtggctgt accattagct actggtgaac gcttgtacac tcgttggcaa
    10981 ttcaagaacc tctttaaaca aggtactgtt gatattatcc aaccagatgt ggctctttgt
    11041 gggggaatct tggaaacccg taaaatcgca gcgatggcgg aagcttacga catggctgtt
    11101 gcgcctcatg cgccatatgg tcctgttgca ctagcagcaa ctctgcaagt agactcatgt
    11161 acgccaaacg tctttatcca agaacaaagc ttagggatcc actataacaa aggttttgat
    11221 ctgttagatt ttgtgaagaa caaagaagtt ttccaatata aagacggcta cgtagatcta
    11281 ccttcaaaac caggtctggg cttggaaatg gatgaagaca aaatcaaaga aattgcacaa
    11341 gaaggtttga tttggaccaa tccgcaatgg aaaaattatg acggtacgat cgctgagtgg
    11401 tgataaatga aaataaagga tgatgaaaga tggcagaagc acttatgatc acagtttttt
    11461 taggactgct gattattttg caaataatcg atcaaaagaa aattatcgtg tttgctcaga
    11521 gtagtcctgt aaggaagagt ttgagtattt tactgacagt cgccatcctg ttgattttct
    11581 ggccggaaca tctgactgat cagattaaac tggttgcttt tgcttctgtc attttgatca
    11641 atggtttttt gaaggagggt ctagcaaaag attctgttgt tagatttgga gtattgaacg
    11701 gtaactttaa tcgatatcct caagtccaag tagaaaaact aggtgacgat aaatcattta
    11761 tttcatttta caaaagcaag aataattctt tttcaatgat ttttgataaa gatcaaactg
    11821 aaatgatgga tgtctttcaa gaacttgata tccattccaa aattacttta gatcaaagtt
    11881 cggaagaaaa aagagcagtt gtaaaaaaag ataatgaatg attctactac cgaaattatg
    11941 atttctttat cacgaataat gaaacttgtt ttaatgaatc atattgattg aagtatacaa
    12001 tgtatattaa gttttggaaa gaaatatatt ggtacaagat atagcaggaa tcgattactc
    12061 gatccctgct ttttttaact tcctttgcag caaaaaaagc tcgtaatcta agagatgatg
    12121 gatagttttt agaatatata tattttcttc aattccagaa aaaaatgacc aagctagtat
    12181 gctaaaaaag tacgatgctt ctatgatctt ttttccgtct ttattaacag atgttttaat
    12241 tgatcaacgc atattgtaat ctaccctaat ttccatagtt cttcaccact tttaaaaata
    12301 gtaaaacccc tacaagtatg ctatagtaaa ttgctaaaga aaaaccaata aggtgggaga
    12361 tgtgtgcgcg atcaaacaag cggaaaaatg attcgttttt attatacaag tttgcatttt
    12421 ctttattgga tgatgttttg ttctgtctat gggtttgcga cattgttttt gatttatttc
    12481 aagattgatc cagctaaaat cggagggatc ttagcattgg tcaatattgt ttcaacggtg
    12541 cttcagcctt ttgtcagtca agtgatcatc aagaaaagaa agatcccact gatcaccgtg
    12601 ctgaagacga tgatagcagg attaggagtg attctcttag ctggggtatt gattccgaat
    12661 ttgccgatca ttacttatat cttgggtggg attttagtag tttctatgca atcatttctc
    12721 aatgcgctgg catttgagta ttcaaatacc ggctacagga tcaactttgg cttttcaaga
    12781 gctggtggtt cattttctta tgctgtaact tcttttttac tgggacagct gttagcgatt
    12841 tattctgccg cgattttgcc aatcttggtt atagttgaag cagtgatatt tttcgttatt
    12901 ttgcatctgc tgccggatgt aaaaaatcaa gaagtttttc ctatgacaaa agaacaggat
    12961 cgctcagttt ctttgagaaa aaaatatcct ttcctgattt tgttattttt aggtttcagt
    13021 tttcttttta ctttccacac catgatcaac agctttttgg cacagatctt tgaaaatatc
    13081 cacggcggca gtaaagaagt tggcatcgca ttgatgatcg ccgctttatg cgaattaccg
    13141 ggaatgatct tttttgaaca gcttgtcaaa aagaaatcca gcaaattttg gctgtctgtt
    13201 gccagtattt catttgctgt cagaggattc atgatgctgc tggccagttc gatcctgatg
    13261 atggaaatga cgcatctcct acaagccttc agtttcgctt tgtttattcc agcttcggct
    13321 tatttgttca atcaatttct ggcggatgat gatcgtgtat ttggacaaac gatgatcatc
    13381 atcgccacaa ctcttggtgg ggtgatagga aatgtcttgg gcggcagtct gctgcagcat
    13441 ttcggtgtat gggagatgct gattttcggt actggttttg cggtgctagg tgctttatta
    13501 actattttgg gcattcgaaa aataccgaag acaggaaacg atagtgattc tcttgttgag
    13561 tagcataaaa atacttagat actagtttta tataaataac atatagtgat tattggcgta
    13621 attacaaaaa acgagaataa cttaagtaag catttctaaa taattgagaa acctcatcta
    13681 aaaagataag aaaatatccc taataaaact tctgtaacat tttagataac tgattgtaat
    13741 ctgaataata aactataagg agacggttag acggtctcct ttttttgcgt attataattc
    13801 tagaattgtt ggaaattgat aataattaac aagaaatatt agacaggaac ttcgaactgc
    13861 ttatcacatt cttattatta tatatttgcg taagtagtcc tggtagtctc aatgcactat
    13921 tctatcttga aactaagaag ttatagaagg agcaatgata gaaagcacga gaatacaaat
    13981 agcgattaaa tatttttcta gacaacctta gttaattcac acagatatat tttaggtaca
    14041 gtcttacaga tatagagtta gtgctgtgtg aatcctgatg ttcgcagtgc ataggagtca
    14101 agtaagattc tagccagtat agaaagtgga agtctggagc gtacctcata atctgggaag
    14161 aaggagcctt ctgatttaaa gccaacaaaa ggaatctcga tatgttccat caaacttgaa
    14221 tgacgattag cagtaagcaa aattgtcccg atctcagctt tataacagtt ttcagctgcg
    14281 gtagtcaatt cctttgtttc accgtttagt gaaacaaaga tcacgatgtc atctttggat
    14341 aattttctac taatgttttt tatgatattg ggatcagtat gcatctcaca atatttaccg
    14401 gtaagttgaa atttgaccat catttcctga gcaatcaatt ctgaaaaacc tcgggcaaaa
    14461 atcatgattc ttctagcagc cttgattcgt tgaatagcat cttcaatatt cccgctttct
    14521 accatattga tggtgcggat tacctcttgc tcatttttaa gaatagcagt ctttattcct
    14581 tcatcaactt tatcaagagc ggcaaaattg atatttgtat tttctttttc ttttaacgaa
    14641 tgtttaaaag acgtaaaccc ctcatatcct tttttcttca tagtacgaac gacggttgct
    14701 gttgaaacgt tggcatattc acttagtttt acaatggaat aggaggggat ttcgagtatg
    14761 tgactttgaa taaaatccca gagatatttc tccgattcac ttaattttgt atccatattt
    14821 tgcctccgat tagttaggat aattctattg taaagcggga gtttccttta cttcaagttt
    14881 atttaatata ttttgtaaaa gttttcaatg aatttagtca tttatgaaaa catttacatt
    14941 cgatcttgat acataatgaa tatgtaagaa aggaggagcg taaatgtcag tttatacatg
    15001 tactttaaac ttggcaatcg acctttttat cgaaacagat gaaatgaaac catcccaagt
    15061 caatcgaaca aacgatgatg atatacaagc taatggaaaa ggtgtgaatg tttcattgat
    15121 catgaagcaa ttaggtatcg atagtacagc tttaggattt aaagcaggtt ttaccgggaa
    15181 ttatattgag gattatttgc atcaaaaaga tattagcact gattttatta gcgtaccggg
    15241 aatgactaga atcaacgttt ttacacaagt taataaaacc aatgaagaat tcaaattggt
    15301 taataaagga cctgagattc ctcaggaaca aatcaaaact tttctaaatc aaatacaaaa
    15361 gctgcaagct gacgattatt tgtgtgtttc tggcagtttg ccgcgagggg tctcacccga
    15421 aattcttatt gatatcagtc gtatttgttt tgaaaataag gtccatctga ttattgattc
    15481 aagctatccg gaaataattg attgtctgcc gtatcagccg ttcttattaa aaccaaacga
    15541 ggaggaattg caatcttggt ataatgtcga gcttagtgat cgtgaaggat atattcatta
    15601 tgctgaacgc ttgattgccg atggagcgaa aaatgttctc ctttctctag gaggagaggg
    15661 aggaatattt ttaaataagg atatcgttta ttctgggaat tctcctaaag gtaaagtagt
    15721 caatacagcg tgtgctggtg acactttgtt gggaacattt cttgcaggat atttaaaagg
    15781 tgaacaggta gatattaatt tgaagaaaag tttagcagct ggaagttcaa ccgcttttcg
    15841 taaaggatta acagattttt cggatgttgc agagttggaa aaacaaatcg aaatcaaaga
    15901 gggagcgagt aaaaaatgac aacttacaaa attgttgccg caacaggatg tccaacagga
    15961 attgcccaca cttatatggc tcaagaagca ttggagcagg cagctaagcg caaaggggta
    16021 tctatcaaag ttgaaaccca tggtcaagta gggatagaga atgaattaac accagaagaa
    16081 ataaaaaacg ctgacgctgt aatcattgca gctgacaaag atgttcaagc agaacgtttc
    16141 gcaggtaagc gtgtaatcga tgtgcctgtc agcaagggga ttaaagatgc tgaccaattg
    16201 atcgaagatg cgttggctgg taaaggttca atcactgcag aaaacaaaac agttgacgct
    16261 gctggtgaga aagcggagat gggggttgca gcaggcattg gtcacagtat ttataaaaat
    16321 ctcatgaacg gtgtttctca tatgctgccg tttgtagtgg caggtggggt aattattgca
    16381 atttcgtttg ccatctgggg tgtgtactct tttgatccca atgatcctac ttacaatccg
    16441 actgccgcta tgttaaaggg tgttggtgat gcggcaatgg gaatgatggt tcctatttta
    16501 gcagcttata tcgctgaagg aatcgctaaa cgtcctggtt tggttgttgg gtttgttggc
    16561 ggactgttag ccaattctgg cggagccggt ttcttaggcg gaatttttgc aggtttctta
    16621 gcaggctact ttatcttact attgcaaaag gtcatgaaag gcttgcctaa acaattggat
    16681 ggtctgaagg cgatcttctt atatccggtg atcggtgtct tgtttgttgg tgtagtcatg
    16741 tctttattag caaatccaat ggaagcagtc aaccaaggaa tgatgaattt cttggcagga
    16801 ttccaaaatt ctaatccatt aattttaggg attatcgttg gctgtatgtc agcatttgat
    16861 atgggaggtc cagtcaataa agcggcttat gttacaggga ctgcgttgtt ggcacaaggt
    16921 aaccatacat ttatggcagg ggtttcggca gcttgtatcg cgcccccatt gattactgga
    16981 tttgccgcac tgttttttgg aaaatatttc gacacgaatg atcgcaacgc tggtttagtc
    17041 aactttatct tgggctcaac gcacattacc gaaggagcaa ttccatttgc ggctaaagat
    17101 ccgctgcgtg ttctgccaac tatgatgcta ggttcatcaa ttgctgctgt actgacatat
    17161 atgtttggtg tccaagttcc cgcacctcat ggaggttttc tagtattacc tgttgttact
    17221 ggtaaggttg catgggtagg gtcgatttta cttggttcaa tcgttggcgg agtaatttta
    17281 gggttgatcc aaaaacgtag tgtagaaaaa cgtgaagcag aagcagctgc tgttgaaatg
    17341 aaagaagcaa ccaacaacta aaggttattc tagaaagaag gatttcaaat gactttgatc
    17401 aaaacaaata aagtttttat gaataaaaat tttacgacgc aagatgaggt aatgaacttc
    17461 cttgcagatc agtcaattgt cttgagcatt agtgaagacc gagatgcagt ttacgaaaaa
    17521 ttgatggaaa gagaagaaga aagcacgact ggtatgatgg atggttttgc catccctcat
    17581 gccaaagatg ctagtatcaa ggatgccgat attgtgattg ttcggttaaa cggtaaggtc
    17641 gattggaaaa gtttagatgg agaaggaacg gattttgtaa tcgctctgtt tattccagat
    17701 tcagaggctg gaacaaccca tctaaaattt ttatctcagg tagccagact tttgatgcac
    17761 aaagatgtga cagaaggtct gaaacaagcc cagtctgctg aagaaatagc tgaactttta
    17821 aaccaaaaat tagaagaaaa ctaaaaagga agtagtgaaa tcatgccttt aacagaaaaa
    17881 aagaaaactt atattcgtca actaagtaat gaaagtggca tcatcagtgc gttagcaatc
    17941 gatcaacgtg gtgccttgaa aaaaatgatc ggtaaatttc aaaataaatc agcaaccgat
    18001 gaacaaatca tcaatttcaa aaaaatcatt tcttctgaat tgactccata tgcttcagca
    18061 atcttattag atccagaata tgggttgccg gcagccgaag ttcgcgataa aaatgctggt
    18121 ctgttgttgg cctatgaaaa aactggttac gatgcttcaa ctccaggacg tttaccggat
    18181 attttatcta tttggtctgt aaaacgtcta aaggagcaag gggccgatgc ttgtaagttc
    18241 ttgttatact acgatgtaga tgaatctgat gacatcaatg accaaaagaa ggcgtttatg
    18301 gagcggattg gttctgaatg tgttgctgaa gatttgccat ttttcttaga attggtttct
    18361 tatgatgcca acgatcctga tacttcttcg aaggaatatg ccttgaaaaa accgcataaa
    18421 gtaaatgaaa tgatgaagga attttctaag cctcgctatg gagttgacgt tttgaaagtg
    18481 gaagttcctg ttaatatgaa ctatgtggat ggttacacta aaaatgaagt tgcttatacc
    18541 aaagaagaag ctgctgctta tttcaaagaa caatccgaga ctaccaacct gccatttatt
    18601 ttcttgagcg caggagtaac tgctgaattg ttccaagaaa ccttgaaatt tgcaaaagag
    18661 gctggctcta cattcaatgg ggtactctgt gggcgcgcaa cttgggcgaa tggtgtagaa
    18721 ccattcgtta ctaaaggtga atatgctgcc aaagaatgga tgcagacaca aggacgcaag
    18781 aacatcgaag agctgaataa tgttttgaaa gaaacagcaa cagctattaa attgtaagaa
    18841 agctttaact ataaccttaa gtttgtaacc taatgatttt taataatcaa tttgagcttt
    18901 taaataaaga attggaagcg tcccgacgct tcctttttcg tttgttttta aggcatgata
    18961 ggttctatgt tgtcgttcaa taagcgcaag aaggtcagca tttttttctt agagtatgca
    19021 aaattgcttt tctacccaat gatttaaaac ggagaggcac ccttggatat ggtagatggt
    19081 gaagtaacgc atttcttctg tgatcacggt ttttttgtct tcttgtagcc agatttttaa
    19141 ataattttct tcgatcagat tatttaatct cttttgaaat tggatatctc cgtggttgat
    19201 gacgaataac ttgtatacga acgtattcgt atagacatag tcgatcaatt gagcgtatag
    19261 atcctcgtag ctatgtgtcg tatcgtagga aatgatttca tccaagtctt ggaaaaagct
    19321 attttccagt ttttcataaa gatcatagat atccgtataa tgtgtataaa atgtcgaacg
    19381 gtgaagatca gcagtttcgg tcagttcacg gatggtgatg ttttgaattt ttttatctac
    19441 taataatttt gataatgctt ctgctaacgc tttttctgtt tttttgacgc ggcgatcctc
    19501 ttttttcatt ttgcagcctc ctaatcaaca taatcaagga attgtgtcgg ttatccgaca
    19561 atttcaccgc tatctgaaca tagataaccg acaccttgtc tattataata taagtatagc
    19621 atatcaatat ttaaagaata ggagaataag tgatggaatt agcctttaag caactggaaa
    19681 agaaagacta caaaaaagcc attcaattcg cgattaaagg tatgcacttt gattggtata
    19741 ccaagaacaa actccttttg aatctttatg gaagatactt ttggtatcta gaattgaatc
    19801 gcgcgacaca aatcattgct ttatatgcgg atgatgagtt ggcaggtgtt ttattagcag
    19861 acatcaaagg aaaatcaaaa aagcatcatt catttagtca aaagttatat gttaaactat
    19921 ttgatttctt acaaaatgca tttgtgaaag acagcaaagg cgggtatgac gacaccaatg
    19981 acgaattgct ttcagactat ttaaaaaaac attccccaga tggagaaatt gtctttttag
    20041 ctgccaaccc tgatttaaag atcaagggga tcggtagtaa attattaaaa gaatttgaac
    20101 gccgagagca aggaaaagaa gtatttctgt tcacggatag cggctgtacg tatcaatttt
    20161 atgaacgacg cggtttcgaa cggaaaaaag aaaaagataa cgtcatgaag atccttaata
    20221 aagaagtaga tctccgctgt atgttatata gtaagatcat tcagtaatgt tttttactat
    20281 cagaaagcag aagtaccacg aaggtcaatg gaaaaagcga gtaattgatc catttagatg
    20341 aagataggtg tagtctcaaa gatattagct ggcaaagata tccccagtta aaaatagatt
    20401 tctgtatcca acaataaaaa tatttttgta tttattagta tatatacttt acaaaaattt
    20461 gtatatatac taattttttt gtaggatcaa tgaaatgagg ggaaaatatg ccaaaatata
    20521 aagaaatagc cttggctcta aaagagagga tcgttgaggg aaaatatcag gaaggttcta
    20581 aattacctga gcaagaaagt ctggctagag agtttaaaac aagccgtgtt actatccgca
    20641 aagctataca actccttatt gatgaaggtt tgttatatac gagacgtgga tctggtactt
    20701 ttatacgaag caatatcaga caggacaatg agaatgtcac tcaagtaaat aatgtatttg
    20761 gcacttcctc acaagaaaat acaaatgtga caagtaaaat aatccgtttt gatgtccggt
    20821 ttccgacaga ttatgaaagg cggctttctg caacctactt ccgatcccct tcctgcaaat
    20881 catgggtata aaaaataggc atattaagcg ggctaggagg agaaatattc acatatcatt
    20941 ttttcattcc gcaagctgcg tcaagttatt tggaatttgt agaacagatg agagaggtag
    21001 aagcagcact acaaactact tttcaataag taggaattat agaaatttta ctcaaaaaat
    21061 cttcattaaa aaatctgact aaataagttg agaaaacaga atatacttca atatccagtg
    21121 atttataaat atcattcact catgataccc agctccgtat cataagtgat gcggttttta
    21181 tcattgatcc taaaaatctt agcaagacaa aaagtacgta agaaagtaaa gactaaatac
    21241 ttcggaggaa caaatgaata acaatgatag gttattgcga ttgcgttatg cgattgattt
    21301 gaaagatacg gatttgatta aagcttttga gctaggcggt gtaccactga ctaaagaaga
    21361 agctcaggcg gtactgacca aagtacagga taagaacaaa gataatgcag aaaataatgt
    21421 gtatgaaaaa accatcaaca accaagtttt tgacgcattc ctaaatggcc tgattctctt
    21481 ggcgcgtggt ccgcaaaaag ataagccgat agtagacacg ccgcaaaaaa gcaaggaaat
    21541 caaatatatc aacaatgtgt tgctaaaaaa attaaaaatc gccttgtcta tgaccactga
    21601 tgatatttta gaagtttttg ctgaggcaga actctatcca tcaaaaggcg aaatcggggc
    21661 atttttgaga aaagaaggcc aacgaaattt taagccttgc ggagataaat atatgcgtaa
    21721 ttttctcaag gggttaggaa tttataatcg gagaaaagta taaagaaatt tcatgagaaa
    21781 tcaaaacaat ggttgttttg atgagagcta gtttttgtat gagattaaaa tattatctta
    21841 atcgaggagc tggattggtt ttatgaatga tgaatataag aatgatgaag ataagatgct
    21901 ttttgaagag atagagaatc gatgtaggct caattttgag cttcggggaa agatgtcttt
    21961 aatacaacaa aaaaagtatt tagcgaataa atcagagttt actctgggac atgttgaaaa
    22021 attaatctcg gattggatta gtagtagaag cgaatttaca aaaataaaac agccgattaa
    22081 atttgacatg aaaaaacttt tgttaaataa gagtgagata ggtaatagag atcaatatat
    22141 tagagctaaa gggcaagaaa taattgattc attaggtgaa atgaggtcat acaattattt
    22201 gtatgtaaca catagagctg atggaatggt cattactgtg gggaaatctt cttctaatga
    22261 tattttttta gatggagatc ttttttatca actcaataca aatcacttgt ctggcactga
    22321 aaatataatt ttaaggacgg aatatggcaa tgaaatattt gcaaaatatg atgagatttt
    22381 aaaaaattat ttggactggg cttggataat tccggttgag tcaggagatg ctaagaaact
    22441 ggagagatta ttgggcgatg aactaattaa taaaaaagta ccaattttaa attattattc
    22501 ccatagacaa taaaaggcta tttttcatga taatactagt tataactgta aaaatggaag
    22561 aaaatctggg aaagaagttt ttaaataagt gtattgttcg attttaataa ataataagca
    22621 ataccatccg tctgtgttgc gcaacatcgt actacaatga atattttttc acaaaaagaa
    22681 aatcatccat cgaatgaatg gaaggtttta gtatacacga ggttaatgcc ccctctttta
    22741 taatcagagg agtgaggttt tttctatgaa tgtttctatt ttcttattgc tagtctagtt
    22801 tctcttacga tgaaattggg attctattcc agtttttatt gatttgctga taaaataact
    22861 tgaatggtgg atataaacat gataaataag agaagaataa caaaacaagg ttgtgaaatg
    22921 attctcaaat gaatataata gaaagaaaaa ggaggaagaa tcaatgaaaa tagcaattgc
    22981 aggcgctggc gcaatgggaa gtcgctttgg gttaatgctt caccaaagtg ggaacgaagt
    23041 attattaatc gatggctggg cagaacatgt ccaacaaatc aaagagcatg gattacaagc
    23101 aaattttaat ggtaaagagg tagaagcaaa actaccaatc gttcttcaat ccgaagtaga
    23161 aaaagaagat caagttgatc tgattattct atttaccaaa gcgatgcagc tggaaaaaat
    23221 gctgcaggat atccaatcat taatcaaaaa agatacagag gtcttatgtc tattaaatgg
    23281 tatcgggcat gaagatatta ttgagaaatt tgtaccaatg gaaaatatct atattggaaa
    23341 taccatgtgg acggcaggtc ttgaaggtcc tggtcaggtc aaattatttg gaagtggttc
    23401 agtagaatta caaaatttag gtgatggaaa agaagcagct gcaaaaaaat tagcagacaa
    23461 attgtctgaa tcaggattaa atgctcattt ttctgacaat attcactatt ctatttatcg
    23521 caaagcatgt gtgaacggaa caatgaacgg actatgcaca atcttagacg ttaatatggc
    23581 tgagctagga aaaacatcga ctgctcataa aatggtggcg acgattgtca atgagtttgc
    23641 caaagtagca gcagtagaga agattgaact agatgtccca gaagtcattg cacattgtga
    23701 atcttgtttt gacccggaaa caattgggtt acattatcct tcaatgtatc aagacttgat
    23761 taagaaccat cgattaacag aaatcgatta tatcaatggc gcaatttcta gaaaagggaa
    23821 gaaatatggt gttgcgacgc cttattgtga tttcttgact gagcttgtcc atgcaaaaga
    23881 agatagtttg aacgtaaaat aaaggagaga ttgaatggaa acttcacaaa aaatatcacc
    23941 gaaaattttt ctgaataaag tcctcgctgg tacagcaaca ggaatcatta ttggattgat
    24001 tcctaatgca gtattaggtg ccattttaaa atattttagt gcgtatccct ttgcggtaac
    24061 gatcgcacag ttagcagtta tttttcaact agcaacacct ttgatcattg gtggtttgat
    24121 tgccttacaa tttggatttg atccgatgag aatgatggtt gtagccggag cttcttttgt
    24181 tggttctggt gtagtcaaat tcacacctga tttgggtgaa acgggtgttt acgttggtgc
    24241 tggtacaggc gatattatta atacgatgct gacagcatcg attgctgtcg ggctgatttt
    24301 actgattgga aatagttttg ggtcagtgtc gattgtgtta acaccgattg ttgtaggtgt
    24361 aggtgcaggt ttacttggtt tttatttatt cccttttgta acagcaatta cttctgcaat
    24421 tggatcattg attaataact tcactacctt acaaccgctg ttaatgtcta tcttgattgg
    24481 ttgttccttt gcctttttga ttatctctcc tatttcaaca gtagcaatcg gaatggcaat
    24541 ccaactagat ggtgtttctg ctggtgcggc tgcgatgggc gtagctgcaa ccacttttgt
    24601 tttagtagtg aactcttgga aggttaataa gtcaggtgtg acaattgcga ttgctttagg
    24661 cgctatgaag atgatgatgc ctaatctatt tagaaaacct gtgattctta ttccttgttt
    24721 agttagtgca gtgattacag ctattcctgt cgctctattt tctatttcag gtacgcctgc
    24781 ttctgctggt ttcggcgtgg tcggacttgt tggtccgttg gcttctttag atgcagggct
    24841 aaatggcttt cttgttgttc tttgttggtt agtcatccct gtttttgcgg cactaattac
    24901 tcaattttta tgcgagaaag tatttaaact gtatgatcga gaagaagtct tcaaattttt
    24961 aggctagtag attttactaa gaaattttca atcaatggat gcgtaagtga gatgtcaaca
    25021 acatctgact tacgtttttt tgcctttttg agtaggagat tgatgctgac aactgaactc
    25081 ttcagtgcca ttcgattttt taaaatgtag aagaaactca cctttatgtt attgattgtt
    25141 caaaaggcaa ctagaataaa aatatgtgtt aaaatgagaa aaaatttgat tgttggatgt
    25201 atcgaataat cactaaaaag taggagaaga aaatgatttt tttgcaaaaa tatggttttt
    25261 attttttatt attaggtgtg attagtgatt tatcgacacc gtatatcctt ggcctttttt
    25321 atcccaaact gaatcaaatg actaccgtaa ttagtgtgtt tggtgacgta gatagtccag
    25381 tcagacgtgc tttcttggtc tggtcagttg tatctggact tttttttgtt ctttcgttgc
    25441 ctgcgttgta tcacctattt attggaacat caaaaacact tgcaatactt gctgttgcaa
    25501 cagttggttt atatgggata ggtgattgta tttttactgg gttattcagt atcaatacga
    25561 atgaaagctc ttggaatcta tcgacttgga tccataatat aggttctggg ctaggctatg
    25621 caggctttct acttttccca ttgttacttg ttctgcttta tcgtcaaagt ggtcaaggta
    25681 cgcttagcca tttttatctt gttttaacag ttattagttt actaattgca ggtctttacg
    25741 gattagcacg gattccgtcg attagtcagt ttgccttctt taagcaattg ggcttctttc
    25801 aacgcttaag ttttttcttt aactatttgc cgatgatttg tttaagtttt tttcaactac
    25861 gaaagtagaa aaagcagtgt gaagctgatt aagtaaaaga atttctctat gggtaaccat
    25921 tcgtgtggat gtgttcgact aagaatagag atgaggagag cgaaaagcaa gttagtctta
    25981 acttgttatg cgttgtgcta aatttcaggt agtcattttt tgaatgaata agaagaaaac
    26041 ttgaaagagg gtgacttcat gttagatgtg gagcagatgg aaatagacct ttataatatt
    26101 attttaaatc gaaatacaag agaatgggaa agaacacaat ttgaagaggc aaaagaagac
    26161 ctagcaaaaa tgtcgataaa gcaggtgata gaaaaactag aggtcaactt gcgtccgcta
    26221 gcactacgag ataatttgac accagatgcg atggattttt atttaagaat cacaggggag
    26281 tcagcgagtg caaaggctta tgattatgcc aaacataaga taacttcacc cttataccaa
    26341 gaacgggcga tttttgccgg tggctgtttc tggtgtatgg ttgagccttt tgaaacaaaa
    26401 ccaggtgtaa tctcagtgtt atctggttat acaggtggag agaccaaaaa accaaattac
    26461 gatcaagtca gtggtggata tactgggcat gttgaagctg ttgagatcat atttgataca
    26521 cgtcaaataa cctatgaaga gctgttggag atttattggc aaatttcaga ccctacagat
    26581 gcctttggtc aatttcaaga tcgggggaaa caatatcgtt caattctatt tgttgttaat
    26641 gaaaagcaaa ggcaattggc tgaaaaatca agagaacaac tgattcaatc taaccgattt
    26701 agccgtccta tcgttacaga gattcgccaa gcaaagactt tttggccagc tgaaaatcat
    26761 catcaacaat tttatcagaa gcaacctaaa agatataaaa agatcaagca ttcgcatcag
    26821 cttcatttat tttttcagcg acatagatag aggaaggttg tggcgcaaag cgttcagctc
    26881 cgacaaaata atgagcaact gacaaaaata gcttttcata tttttggaca gttatgaatt
    26941 atttcgagga gttgctttga gaacaccgag gaaaaggttg tggcgcaaag cattcagctt
    27001 caagtcgcaa ggaaacagga ggtatgacaa gtcttttgtc acaactcctg tttttttact
    27061 agcctttatt tccatgtgaa agagaattcg aattgtagaa tcttgatttt tctatggaat
    27121 tttgtagaga atcatcttga taattaatag acacctaatc gaattccaca ttaatgatga
    27181 actaaattcc gtataaaagc gcaaacaata acta
//
LOCUS       DACEPD010000031        26378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.031, whole genome shotgun sequence.
ACCESSION   DACEPD010000031 DACEPD010000000
VERSION     DACEPD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.031"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(81..776)
                     /locus_tag="IW797_001255"
     CDS             complement(81..776)
                     /locus_tag="IW797_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ1224040.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(866..1966)
                     /gene="ychF"
                     /locus_tag="IW797_001256"
     CDS             complement(866..1966)
                     /gene="ychF"
                     /locus_tag="IW797_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ1224041.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(1975..2166)
                     /locus_tag="IW797_001257"
     CDS             complement(1975..2166)
                     /locus_tag="IW797_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ1224042.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2187..3077)
                     /locus_tag="IW797_001258"
     CDS             complement(2187..3077)
                     /locus_tag="IW797_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ1224043.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3064..3831)
                     /locus_tag="IW797_001259"
     CDS             complement(3064..3831)
                     /locus_tag="IW797_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1224044.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3843..4556)
                     /gene="rsmG"
                     /locus_tag="IW797_001260"
     CDS             complement(3843..4556)
                     /gene="rsmG"
                     /locus_tag="IW797_001260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ1224045.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4788..6689)
                     /gene="mnmG"
                     /locus_tag="IW797_001261"
     CDS             complement(4788..6689)
                     /gene="mnmG"
                     /locus_tag="IW797_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ1224046.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6706..8103)
                     /gene="mnmE"
                     /locus_tag="IW797_001262"
     CDS             complement(6706..8103)
                     /gene="mnmE"
                     /locus_tag="IW797_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ1224047.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8447..8785
                     /locus_tag="IW797_001263"
     CDS             8447..8785
                     /locus_tag="IW797_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224048.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(8953..9711)
                     /locus_tag="IW797_001264"
     CDS             complement(8953..9711)
                     /locus_tag="IW797_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1224049.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9729..10547)
                     /locus_tag="IW797_001265"
     CDS             complement(9729..10547)
                     /locus_tag="IW797_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1224050.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10544..10900)
                     /gene="rnpA"
                     /locus_tag="IW797_001266"
     CDS             complement(10544..10900)
                     /gene="rnpA"
                     /locus_tag="IW797_001266"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ1224051.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11018..11152)
                     /gene="rpmH"
                     /locus_tag="IW797_001267"
     CDS             complement(11018..11152)
                     /gene="rpmH"
                     /locus_tag="IW797_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ1224052.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11676..13010
                     /gene="dnaA"
                     /locus_tag="IW797_001268"
     CDS             11676..13010
                     /gene="dnaA"
                     /locus_tag="IW797_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ1224053.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13208..14338
                     /gene="dnaN"
                     /locus_tag="IW797_001269"
     CDS             13208..14338
                     /gene="dnaN"
                     /locus_tag="IW797_001269"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ1224054.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14567..14809
                     /gene="yaaA"
                     /locus_tag="IW797_001270"
     CDS             14567..14809
                     /gene="yaaA"
                     /locus_tag="IW797_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ1224055.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14796..15920
                     /gene="recF"
                     /locus_tag="IW797_001271"
     CDS             14796..15920
                     /gene="recF"
                     /locus_tag="IW797_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ1224056.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15917..17863
                     /gene="gyrB"
                     /locus_tag="IW797_001272"
     CDS             15917..17863
                     /gene="gyrB"
                     /locus_tag="IW797_001272"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ1224057.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17883..20354
                     /gene="gyrA"
                     /locus_tag="IW797_001273"
     CDS             17883..20354
                     /gene="gyrA"
                     /locus_tag="IW797_001273"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ1224058.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20590..20889
                     /locus_tag="IW797_001274"
     CDS             20590..20889
                     /locus_tag="IW797_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ1224059.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20939..21451
                     /gene="ssb"
                     /locus_tag="IW797_001275"
     CDS             20939..21451
                     /gene="ssb"
                     /locus_tag="IW797_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1224060.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21477..21713
                     /gene="rpsR"
                     /locus_tag="IW797_001276"
     CDS             21477..21713
                     /gene="rpsR"
                     /locus_tag="IW797_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ1224061.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21856..23829
                     /locus_tag="IW797_001277"
     CDS             21856..23829
                     /locus_tag="IW797_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ1224062.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23835..24287
                     /gene="rplI"
                     /locus_tag="IW797_001278"
     CDS             23835..24287
                     /gene="rplI"
                     /locus_tag="IW797_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ1224063.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24561..25928
                     /gene="dnaB"
                     /locus_tag="IW797_001279"
     CDS             24561..25928
                     /gene="dnaB"
                     /locus_tag="IW797_001279"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ1224064.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         7999 a         5199 c         5227 g         7953 t
ORIGIN      
        1 attatttaac tatggataaa caaaatgagg ttctttacta cccctagata tctagcaaaa
       61 tagctgatct ttttgctagt ttatcttctt gggacggata aacttccaat aatcccgtat
      121 tttttcttca agtagtaacg gattgctaaa gcagttgccc cgataatgat aactactact
      181 ggatctaata ctggattgat aactggtggc aatagtgttg tcgcagaaaa cagtacgacc
      241 caaagaagtg tcgttccacc taggataagc aatgtcttac ccattcctgg tttcttgctt
      301 cgatctgctc cgggatattc atattgatag atgtatttgt acatcatata aaagacccag
      361 ccaccgattg cagaacctag aacgagtgta gaaaagccat aagctggcgt tgttccacgt
      421 gaaaacaatc cggtaatcgc aatcatgata cttaatagac caagcaacag caacgtatta
      481 tctaaccaca tcaataaagg ggtggatgaa ttttttactt ctgctggctt attgatgatt
      541 gcttccgttc tttcagagac tgtcccaaac aattgacggg ctgttcttcc gcctttttgt
      601 tctgcaacta aagtaggcag aatgtcatga agggctttta ctttttcttc ttcagacaaa
      661 ttcgctgctt ctaaagattt tttcaaatca aaaatgtact gttgatttct tttggttaat
      721 tttgtttcta aactattgtt ttcttttacg attgctcgta attgttctgg ttccattatc
      781 taatagtcct ccttagccgt tccttttctc aacggtcgtc acaaaagaca ttctaccata
      841 ttctacagct agtttgatag cccttttaca cattaaagcg gaataacatt acgtcgccgt
      901 cttgtacgac ataatctttt ccttctaatc gaacacgtcc agcttctttt gctgcatgca
      961 tgtttccgta atgatccaag tcttcaaaag agacagtttc tgcacggata aatccgcgtt
     1021 cgaaatctga atggatgatt cctgctgctt gtggtgcttt gattcctttt ttgaatgtcc
     1081 aagcacgtac ttcttgttcc ccagcagtaa agtaagtagc taagcctaac aaatcataag
     1141 cagcacggat caactgatcc aaacctgatt cttcgattcc taaagcttct aaaaattccg
     1201 ctttgtcttc ttcatctaat tctgcgattt cttcttctgc acgagcactg accacgatca
     1261 cttctgcatt ttcttctgca gcaaagttgc gcacttcttg tacataatga ttcgcgtctg
     1321 catctgctac gtcatcttct gagacattgg ctacataaag aattggtttt gtcgtcaata
     1381 aaaatagtga tttaacgatt ttttgttctt cttcagtaaa ttcgatcgtt cgggcagatt
     1441 ttccttcttc cagtacaggt ttgattttat ccagtacagc tagttctgcc actgcatctt
     1501 tatctttggt tttagcaact tttgctacac gagtatagcg tttgttgata gattctaagt
     1561 ctgctaacac tagttccaag ttgatcgtat caatatcttc tagtggatca acacgtcctt
     1621 ctacgtgagt aatattatca tcatcaaaac agcgaacaac gtgacaaata gcgtctactt
     1681 ggcggatatg gcttaagaat tgatttccca aaccttctcc tttacttgcg ccttttacga
     1741 ttccagcaat atctgtaaat tcaaaagtcg tcggaacagt ttttttcgga tgtacaagtt
     1801 ctgttaagcg ttgcaaacgc cagtcaggta cttctaccat ccctacgttg gggtcgatcg
     1861 tcgcaaaggg atagtttgct gcttctgctc ctgcttttgt gatcgcatta aacaaagttg
     1921 attttcctac gttgggcaac ccaacaattc cagcagtcaa tgccataaat ttattcactc
     1981 tttctctgca tttttttcaa ttactttttt taatttcttt tcaaaatctc gacgggtcat
     2041 catgacgata tgtccgcaat tcatacatct gattttgata tctgcgccca ttcgaatgac
     2101 ttcccagcga ttcgtctggc aagcgtgagg ttttttcatt tcaacgatat cgtgcaagtc
     2161 gtacataaaa atagcctcct ttttgtttat tcttcatcaa attgaatatt caagatatca
     2221 agaattcgag tcaaatcttt ttgagaaaga tattcaatct cgatcttacc tttcccattt
     2281 ttttcttgga tagctacact tgtaccaaat ttatccatca aacgatcttc gctttctcgg
     2341 atataatatg gtttttcctt tgctagcctt gggatttttt tcttttcttt tccatcattt
     2401 tgatttaact cagaaacaag ctgttccagt tggcggacag ttaaattttc agttactgca
     2461 cgattggcta atttcaagat caatgtttta ttttttaaac ctaacagcgt acgggcttgt
     2521 cccatagata aacgctgatc ttgtaccatt tccttgacaa gatccggtaa agaaagcaag
     2581 cgcaaataat tggcgatata aggtctgctc tttcctaaac gactagctac ttctgcttgt
     2641 gttagtttca ggtttttcat cagcatctca tatgcttctg cttcttctaa cggattcaga
     2701 tcttctcgct gcaagttttc cagcactgct acttgcatca tcgcttcttc gtcaaagtct
     2761 ctaacgatag cagggatcgt ttctttttca gctagtttgg acgctcgaaa acgtcgctcc
     2821 cctgcaataa tctcatatcc ttttactgcg gaacggcgta cgatgatggg ttgaaagaca
     2881 cctgattgtt caatagaatt agctagttcc tgcaaagata cttcttcaaa tgttttacgg
     2941 ggctgatacg gatttggtcg cagctcattc aatggtagct ggacaacggt atcatctttt
     3001 acatccaccg tctctaagtt ttctaaatct tgaaacagtg catcgattcc tcggcctaat
     3061 cctttacttt tcttcacgac ttaccacttc ctttgccaat gcttggtaga cttcagctcc
     3121 tcgagaacgt ggatcgtagt cgatgatcgg ttttccatga ctaggtgctt ctgataaacg
     3181 aatatttctt ggaatgatcg tttcgtaaac tttttcgcgg aagtatttac gtacttcttc
     3241 tacgacttca tttcccagat ttgttcgtgc atcatacatt gtcagtaaga ctccctcgat
     3301 ctctaattct ggattaaagt gtttttggac taaacgaacc gtattcagta attggcttaa
     3361 tccttccaat gcgtaatact cacactgaac aggtatcaaa atcgaatcac ttgctgtaaa
     3421 tgaattgatt gtcagatggc caagagaagg cgggcaatcg atcaaaatat aatcatactg
     3481 actgcttact tctgctaaag atccttttaa tcttgattct cttgccatca ttgacgtcaa
     3541 ttcgatctct gctcctgcta attggagagt cgcaggcaca atactcaaat tttcgtgttc
     3601 agtgatcaaa gttgcctcat caattggcaa ttcattgact agaacatcat agatgtctct
     3661 tgtcacatca ggttttctga tcccgacacc actggtagca tttccttgtg catccatatc
     3721 cactagcaat acttttttgc caaggctagc aagacaagca cctagattca ccgtcgtcgt
     3781 tgttttgccg acgccacctt tttggttcgc aacagatatt atttgtgcca tctcttctcc
     3841 tcctatttga ttggctgctt attaggcagt ccgggctttc ttgggtattt ctttggggtt
     3901 ggtttctttt tttgaataac gatgatatgc cgttgatcag agacattcgg caaggtaacc
     3961 gcctcttctt tgaggaattt accacctaat aaagcaatcg cttgttttgc ttctgtcaat
     4021 tcctcttcac tttttgctgc tttcaatgca taaaaatagc cttcttgttt agccaagggc
     4081 aaacacaatt ccgctaacac attcaaacga gcaaccgcac gagcagtgac aaggtcaaat
     4141 gatgcacgaa agtgaggatt ttgcccaaat gtttctgccc ggtcatgata taaggaagca
     4201 tcgattccta ataatcgggt taactcagat aagaaggtta ttcgtttatt cagagaatca
     4261 acgatcgtta tctgcagttc tggaaaggca atttttaaag gtatgctagg aaaccctgcc
     4321 cccgaaccaa catcgcataa agataaaggt tgcgtaaagt ctacttcaaa tgctaatgta
     4381 agtgaatcgt aaaaatgctt cagatatacg tctttttcat ccgtaatagc tgtaaggttc
     4441 atcttttcgt tccattcgac taatgtccga taataaatag cgaattgatt catttgatga
     4501 tcggtaagct ctatcccttt ttctgccaag gattggcgaa attcttctgg tatcataaat
     4561 attcctttca ttgtgaaaca gcaattgttt cacagattct ctccctactt taacgcaaaa
     4621 tctctagaca tgcaatcctt ccatatggat tcttaaaaat ttgaaaagta agaaaagccg
     4681 atagactttt tctttctagt atgactcata aaatatacac agaagagaga actagctttg
     4741 agcaatttct gcaaaaacta gttctctctt ctatttatta ttacgtatta cgctggttca
     4801 ctgactttcg caatcttccc ttgttcgata tagaccatta ggatacttag gtctgcaggg
     4861 tttacgccac tgattcggct agcttgggca attgtttctg gttggatttt ctgcaatttt
     4921 tgttttgctt ctgttgctaa tccgttgatt gctgcgtagt cgatgttttc tggaatcttt
     4981 ttcgcttcca tgcgttttaa tttttctact ttttccatgg cttttttgat gtagccttca
     5041 tatttgattt ggatttctac ttgttcgatt tcttttcgtg tcaattcttc attttctgga
     5101 ataaaccgta gaagttctgc ataagtaatc tcaggacgtc ttaaaaagtc gcttgctagt
     5161 acaccatctt ttaatggtgc cgcattaatc gttgctaaaa agtcttggac ttcttctgtt
     5221 ggtttgattc gtacattcat caaacgtttg atttcttgtt caactgcttc ttttttcttc
     5281 aaataattct tgtattgatc ttcttttact aagcctacac ggtgacccat ttctgttaat
     5341 cgcaaatctg cattatcatg acgtaaaagc agacggtatt cagcacgtga agtcagcaaa
     5401 cgataaggtt cgttcgttcc ttttgttacc aaatcatcga tcatgacacc gatgtaaccg
     5461 tcacttcgtt tgatcactaa aggttctttt ccctgaactt tcaaagctgc attgataccg
     5521 gcaatcagcc cttgtccagc cgcttcttca taaccgcttg taccatttgt ttgtccagct
     5581 gtataaagat tttcaacgac tttagtttct aatgtcggac ggagctggtg tggctctact
     5641 acgtcatatt cgatagcata tcctgtacgc atcatttcta ctttttctaa tccagcaata
     5701 gaatgaagca tgttgacttg aacatcctct ggtaaagaag tagataaacc ttgaacatag
     5761 acttcttccg tatttaagcc ttctggttcc aaaaataatt gatgacgtgg tttgtctgca
     5821 aaacgcacga tcttatcttc gatggatgga caatagcgag cgcctacccc ttcaacgatc
     5881 cctgtaaaca ttggggcacg atgtaagttg tctcgaataa tttggtgagt cgtctcacct
     5941 gtataggtca gccagcaaga ttcctgatct agtttatagg cactatctgg tgtgctgaag
     6001 ctaaaatgat tcggctcttt gtcaccaggc tgctcttctg taacagaata gtcgatcgta
     6061 cttgatttta ctcgaggcgg tgttcctgtt ttgaaccgtt cgatagcaaa gccaagttct
     6121 tttagatgat tcgctagtcc aatagaaggt tgagagttat tcggacctga tgaatatttc
     6181 aattcaccga taatgatttc tccgcgaagt gctgttccgg ctgtaataac aactgctttg
     6241 gcccggtagc tcgctcctgt ggacgtcact actccatgac aaacaccatc ttcgacgatc
     6301 agttcttcga cgattccttg tcttagcgtc aaatttggtt ctttttcgat cgtatgtttc
     6361 atttctgttg aataagcatg tttgtctgct tgtgctcgca atgctcggac agctgggcct
     6421 tttcctgtgt tcaacatacg catttggata taggttttat cgatattctt acccatttct
     6481 ccgcctaacg catcgatttc tcgaacgaca acgcctttag ctggtccgcc tacagaagga
     6541 ttacatggca tgaatgctac catgtccaaa tttattgtca gtaataatgt ttttgatccc
     6601 atacgtgcag cagcaagtgc agcttctgaa ccggcgtgtc ctgcgccaac aacgatcacg
     6661 tcatacgcat ctgcttcata atgttccaat cgtttgttcc tccttttatt ttcctaaaca
     6721 aaattgactg aataattgag tgattagctc atcttgtaca ctgtcgccga caacttctcc
     6781 tagatagtcc cagcaacgcg tcatatctat ttgaacgaga tccacaggca ttcctgaatc
     6841 aatacctgca atgacttctt caagtgagag ggaagctttt tctaacaacg cgatatggcg
     6901 ggtattagaa acataagtag cgtctctttc tcctgtttct ccgctgaaga ataaatcaga
     6961 aatagctgat tccaaacgat ctagtccgtc gtttttagct acggaaattg aaaaaactgg
     7021 ttcattttcg atcaatttct tcaatttttc ttgttccagc tgtgctggca aatctgtttt
     7081 atttagaaga atgatccgtt tcaatccact tgtcgcttcc aataattgtt catcttctgc
     7141 tgtcagtggt tcgctttggt ttagaacaag taagatcaag tctgcttctg ctaatgcttt
     7201 tcggcttctt tcgaccccga tccgttccac cacatcttct gtttcccgaa ttccagctgt
     7261 atcgattagc ttgagcggta ccccccgaac attgacatac tcttcgatta catctcgagt
     7321 cgttcctgca atatccgtaa cgattgcttt ttcttctctt aacaaatggt ttaacaaact
     7381 tgatttgccg acatttggcc ggccgatgat tgccgtactt aacccttccc gcagaacctt
     7441 cccttgcttg gatgtagcaa gtagcgcttg gattctttgt tggatcatct gcgctttttc
     7501 aagcaataac ttcgtagtca attcttcgac atcatcatat tctggataat caatatttac
     7561 ttctacttgt gctaatgtct ctaaaatctc ttgtctcaat gagcggatca aagcagacag
     7621 attgccgtct aactgattaa gagctagtcc cattgctttg tctgtttttg cccgaatcaa
     7681 atccatcaca gcttctgcct gtgaaaggtc aacacgacca ttcaagaatg ctcgttttgt
     7741 gaattcgcct ggctcagcta atcttgcacc ttctcttaac aatagctgca aaatttgatt
     7801 gactacaacg atcccaccgt gacaattgat ttctactaca tcttcccgcg taaatgtctt
     7861 tggtgctctc atcacggaga ccatgacttc atcgacaagc tgattgctct tgggatcaac
     7921 gatatgtcca taatggatcg tatgacttgg tacttctgat aatcgtttgt ttccagattg
     7981 ataaacttta tctgctagtt gcacagcttg atcgccactt aaacgaacga tactgattgc
     8041 accttctcca ggtggggtag aaattgccgc aatcgtatca aattctaaag taatttcagc
     8101 cattttttca cttccttttt cattttgcgc acaaaaaaag tgcccactcc accctttatg
     8161 cacaataaac ggtgaacttc gcactttgac tgcttgttct tgattaaatt ctggtcatag
     8221 attatcacac ttgctataag gaatcaagtg tttaaaacaa ttagatgaag ttgagcctgt
     8281 tctgcttcca ttaccttata tttaattgtc cttggatgtc tctttgcgtt ctttggttta
     8341 ttcgaagaga agatactgta taaacaaggc tttatctttc ttaaaaaaaa cggtacacta
     8401 aatggtagcg ctatctaaac tatacacttt tgaaaggaga aaatatgtga gcccgttcag
     8461 atctgctgca ttggaagaag aaagtgaccc tcggatcaac gatgtcgttt tatctacagg
     8521 gaatatcaac cagaaatata tcgtccgcga tatcgttttt gttgtggaat gcttagaagc
     8581 tgatttgttt gacccatctt ttcccaaaga tgaccttttc ttaacaacga agaggaaatt
     8641 aaagacgaag gtacttgaat atggtgcgaa tgcagtaatc aactgtcatt ttgagtatca
     8701 gcaaaaaaaa cgcgacggaa agtattttct ggaacttttt gcttatggaa cagtgatcca
     8761 atttatccaa acaacgattg gttaaatgaa acagacaaaa aaagcggatc aaaactgaag
     8821 acagtttcga tccgcttttt tctatcaaag ttagtgacta cttcatcgta tactctgata
     8881 agaatgattt ctagtaaaat atacttggtt tgaaaaactt tgtcaacgtg atttattcaa
     8941 aaagtaatgg aatcaataat attttttagc aggttctact actaaataac gataaggttc
     9001 ctcaccttcc gaatgtgttt ttacatgttc atctttgctt aatgcagcat ggatttgttt
     9061 acgttcaaat gcaggcattg gttctaagaa aacaggctgc cctgtccgtt ttactttttc
     9121 agccgtgcga tcagctaaac gctgaatgat ctcttggcgc ttttcacggt aattgcctac
     9181 attgactact acagacagct tatttcttgc taaacgatgg atgaagactt gtgccagata
     9241 ttgcaatgca ttcagtgttt ttccatgttt cccaatcagt atgccttgct tttgtgtttc
     9301 taaatggaaa acaaccatat tatttgattc tctttccatt ttgacaagag ctggagcatc
     9361 aagctgtttt gaaatttcag taagataaac tgccaattcc gttaaagctg cttcatcacc
     9421 tagctcactt acttcttctg ttttttcttc aagaatcgtt tcgttgacta cctcgatctc
     9481 agctgttggt tcttcttcca aaatttcttc tacagtctgt tctactgttt cagaaacttt
     9541 ttcgctgata cttggctgga tcgaaagccg tgcattcttt cgacccattc ctaggaaacc
     9601 tttttttcct tcttccagta tttcgatttc gacttggtct ttggccaaac ccaaatcagc
     9661 cagacctagt tggacagctt cgtctattgt tgcagcttca taaatcggca ttagcattac
     9721 ctcccctatt atttccgttt tttcttagga ttcattgctt tcttaagagc acgttctctt
     9781 tcgcggattc tccgtgcttc ttcttcacgc tcttttcgaa ttttgaaagg gttgttgatc
     9841 agcagcgttt gtacaacttg gaaggcattg gaaactaccc agtataatga aagaccgctg
     9901 gctaaattga cacccatcac taaaatcatt acaggcatca cgaaattcat gatcttcatt
     9961 gatgcattcg attcaatttg actcatgcta gataaatacg tacttgcaaa agtaaagatt
    10021 gctgctaaaa tcggcaaaat aaatgttgga tccgcgttcc ctaagttcaa ccataagaaa
    10081 tgcccttgtt tcaactctgg tacacgtgaa atagattgcc ataacgccat caagattggc
    10141 atttgtacta ataaaggaag acaaccagca tatggattga cgccattttc tgcatataaa
    10201 cgctgctgct cttctcttaa cttactttgt gtttccggat ctcttgaact atattgttgc
    10261 tgcagtgctt ttaatttcgg ttgtaattct tgcgttttac gcatgctctt tgtttggaaa
    10321 tgcatcaacg gcaataggat cacgcggatg atcagtgtaa ataaaatgat ccctatccct
    10381 gcattaccaa atgacaacgc cttgatcgcc tcagcaaagt aataaacaat ataacgatcc
    10441 caaattcctg tactctgtgc gctgacttcc ccagttccgc aaccggataa gacgatgacc
    10501 aaactaacga taccggccat caaaagtaaa cgtttgtatt tcttcactta ctcttttcct
    10561 ctctgtcgat tatttttgca agttttaaga cgtggactaa attggagcga acctcatcat
    10621 aagtcaaccc cttcacactt ggtctagcaa tcacaataaa atccaactcc ggatcaattt
    10681 cttcttttat agaataaact gctgctcgga tttttcgttt gacttcattg cggtttaccg
    10741 cgttgccgac cttttttcct acagataaac caactcgaaa atgaggctgt cccggttttt
    10801 ccaaacggta aacgacgaat ttgcggttag caaaagaatc tcccgtatgg aacacggctt
    10861 gaaactctcg ttcttttttt actcgatacg cttttctcat tagttttctt tccttttcta
    10921 tctctcactt acgctcataa tcataccata attacgattg tcctttcatc atttccctaa
    10981 aaaagagtat aaaaaaaacc actgaaaccc cagtggccta agctgaaaga acttttcttc
    11041 ctttgcgacg acggctagct aacacacgac gtccattttt agtactcata cgtttacgga
    11101 atccgtgaac tttttgacgt ttacgtttat ttggttggta tgttcttttc atatattcca
    11161 cctccaaaat gttcttatct attttataaa cacagacata ctttgatagt ataacgacaa
    11221 agtagtgatt ttgtcaatat aaaatagaag atcgattgtt aaaacttatc cacaagatta
    11281 tttttttcta tgggagtcct atgtggaaaa agttatgcac agggattatc cacagataat
    11341 aaatggctgg ttttacacag aaaacacacg tgtaaaaaaa gtaatccaca atcaggtgat
    11401 ttcctgtgta ttccgtctcc taactattgg tatatctata ttttttatcc acagatgcaa
    11461 gaggaaatct attgattttt acgagttttc cacctgtgca ttagtggtgt ggaaaacttt
    11521 tttttgacaa ctgtgttttt ctcttttttc tttttgtcga ttgtgtggat aattgtaatt
    11581 aaaaaatttt tttcttcggt ttctgtggaa aactattgaa aaaaatgcta aattagtact
    11641 agttattcac tttttaatga aggaggaggc cttatatggt atccctcgat gctttatgga
    11701 atgaattaaa agcaacatac caaaaggatt tatcgcctgc tagctataat acatggatcg
    11761 aaacagcgaa ccctcgaacc cttgatcaaa accagctcgt tgttgaggtt cctagcaaga
    11821 ttcataaaga atattgggaa aaaaacctgg cgacaaaaat cgttgaagtc ggttatatgt
    11881 tatcaggaaa tgagattatc cctcgcttta ttactggcga agaagcagag caagaagaag
    11941 ttatagaaga aaaaaatcca aaagttgtgg cacctagtcc gctaaaaaaa gccatgctga
    12001 accctaaata tacctttgat acgtttgtca ttggtaaagg aaaccagatg gctcatgcag
    12061 cagcgctcgt tgtagcagaa gatccaggtt ctatttataa cccgctcttc ttttatggag
    12121 gcgtgggact tggtaagact cacttgatgc atgcaattgg tcatcaaatg ctgcaaagtc
    12181 agcctaatgc caaagtaaaa tatgtaagca gtgaaacttt tgctaatgat ttcatcaatt
    12241 cgatccaaaa caaaacagca gaagagttcc gtcaagaata tcgaaatgtt gatttactgc
    12301 tagtggatga cattcaattt tttgcagaaa aagaagctac gcaagaagag tttttccata
    12361 ctttcaatgc cttgtacaat gaagggaaac aaatcgtact aaccagtgat cggttgccaa
    12421 acgagattcc gaaactgcaa gaacgcctcg tttctcgctt tgcatggggg ctgtctgttg
    12481 atattacacc gccggatctt gaaacaagaa cagcgatttt acgtaaaaaa gcggcggctg
    12541 aacgtctaga gatcccagat gacacactaa gctacatcgc aggacaaatt gattctaaca
    12601 ttcgtgaatt agaaggtgcg cttgttcgag tgcaagcttt tgctgcgatg aatagtgaag
    12661 atatttcgac tagtctagct gcagacgcac tgaaaacgtt aaaatcagga aaaggacatc
    12721 ctcaattgtc aatcctgcag atccaagaag aagtggcaaa gtattatcac atccaattga
    12781 aagatatcaa aggaaagaaa cgcgtcaaat ctattgttgt tcctagacaa atcgctatgt
    12841 atttggcacg tgagctcaca gataattctt taccgaaaat cggagctgaa ttcggtggca
    12901 aggatcatac aacggttatc catgctcatg aaaaaatcca gcagctgatg gattctagtg
    12961 tcagtatgca aaatgaagta tcagaaatca aaaacttttt actaaattaa tttgtcgatt
    13021 ctatcaactg aaaatagcat gtggataaaa gaaaaaaacg ttcaaaagtt atgcacactt
    13081 tttccacagc tgttttttcg tttctctctt acgttttttg agttttccac agaatcaaca
    13141 tgccctatta cttttattat tctttttaac taataataaa taaacaaaca ttcaaaggag
    13201 atacatcatg aaagttactt taaaccgagc tagctttatg caggaattgc aaactgttca
    13261 acgagctatt tcaagcaaaa ccacgatccc tattttgaca ggtgtaaaaa tcacactgac
    13321 acaagaaggt ttgactttga cggggagcaa cgctgatata tcaattgaaa cttttttgtc
    13381 tgttgaaaac gaaaaagcaa atatgcaaat cgaatctact ggttccattg ttttacaagc
    13441 acgtttcttt agcgaaatca ttcggagact tcctgaagaa acatttactt tagaagtttt
    13501 agaaaataaa caagtagcga ttacttctgg aaaagcgaat tttatcgtaa atggattaga
    13561 tgcagataac tatcctcatc ttcctgttgt cgaaagccat aaccagatga aattacctgt
    13621 acacgtattg actaaactaa tcaacgaaac agtttttgct gtctcacaac atgagagtcg
    13681 tccaatcttg acaggtgtgc atttcatttt atctgataat tctttattgg ctgtagctac
    13741 tgattctcac cgtctaagtc aacgcgtgat tccagtagaa caagcggctg atcattttga
    13801 tattgttatt cctggaaaaa gtttgatcga attatctcgc tcattaacaa atgaagaaga
    13861 aatcgtcgaa atcagcatta tggaaaacca agtgttgttc aaaacagaaa cgatgtattt
    13921 ctattcccgt ttgctagaag gaaactatcc tgataccaat cgtttgattc cttcaagttt
    13981 taatacagag gttgaatttt ctgttcctag ctttttagcg gcgatcgaac gggcttcttt
    14041 actttctcat gaaggccgta acaacatcgt tcgtttatct attcgtccag atgcggttgt
    14101 tttatacgga aattcgccag aaatcgggaa agtagaagaa agcttgagtt atacagcaag
    14161 cagtggcgat ccattagata tttctttcaa tccggattat atgaaagcag cgttgcgtgc
    14221 atttggagat atgagcatca aagtgaaatt tatctctgct attcgtccat ttacattgga
    14281 accaacggaa gatggcgttc aatttattca gctgattact cctgtacgta caaattaaca
    14341 atgattttca ttgttcttga aaatttaatt gaaaagaaat agaaaaaata aaattttaaa
    14401 cagaaggccc taaaatctct tttaaggctt tctgtttatt tttgtttaaa tcaccttttt
    14461 tagattaaat cgtcttatat ggaaaaatat atggataaat ttgttttatt gcgaaaaaaa
    14521 aggtataata gagtatactt ttaataatga aaatgaggga tgggcattga aacaacaagt
    14581 cattctaaat acggaattca tgacgttggg ccaaatgttg aaagaagtca cagttatcgg
    14641 tagtggcgga caagcaaaat ggtatttggc agaaaacact gtgttagtcg atggcgaacc
    14701 agaaaatcgt cgtggacgca aactttatcc cggcatgatg gttgaagttc cagaagtagg
    14761 aacttttttt atggtaaaaa aaggaaacga cgataatgag gctgaataag ctgtatttaa
    14821 aaaattatcg aaattacgaa gagctgaaca tcgaattttc caagaacctc gtcatctttt
    14881 taggagaaaa tgcgcaagga aaaacgaatc ttttggaaag tatctacgta ttagctatga
    14941 cgcgaagtca tcggacaagt agtgagcagg aattgattgg ctggactgac gatcaggcga
    15001 tgattcaagg agagatcaca aaaggttcat ccactcttcc attagaaatc ttattatcta
    15061 aaaaaggccg aaaaaccaag atcaaccata tcgaacaaaa aaagttaagt acttacgtgg
    15121 gacaaatgaa tgtcatttta tttgcaccag aagatctttc tttagtaaaa ggcagcccgc
    15181 agatcaggcg gaagttttta gatatggaat taggccagat caatccaatc tatctttatg
    15241 atcttgtcca ataccaagca attttgaaac aaagaaacca gtatttaaag caattgaatg
    15301 aaaaaaaaca aactgatttg ctttatttgg atattttgtc cgaacaacta gcagcgtttg
    15361 gcagcaaagt attgaaagca agagcacagt tcgtcaagcg attggagttt tgggctaatt
    15421 ctttgcatca gcaaatcacc catcaaaaag agcagctgga aatcgagtat ctcaccgcag
    15481 tcgattcttt agaaacacat acgcaagagc aaatccaaga acaatttctc gcacttttga
    15541 accaaaataa aaagaaagac ctttttcggg gaacaacgac agttggtcct catcgtgacg
    15601 atctttcctt ttttattaat cagaaaaatg tgcagactta tggatcgcaa ggacagcaaa
    15661 gaacaactgc actaagtgtc aaattagcag aaatcgattt gattaaagaa gagacggggg
    15721 aatatccaat ccttttgtta gatgatgtga tgagcgagct ggatgatagt cgtcaactcc
    15781 atctgctaga aacaatcgaa ggaaaagttc aaacgttctt aacaacaacg acacttgacc
    15841 atgtaaaaaa taaaatgact gttgaacctg agatttttta tgttcaacaa gggaaactaa
    15901 aaggagaaga aactgaatga cagaagaaag aagtttagta gaacgcgcta aagagtatga
    15961 tgccagtcag attcaagtac ttgaaggact tgaagccgtt cgtaaacgac ctggcatgta
    16021 tatcggttcg actagttctg agggtctgca tcaccttgtt tgggaaattg tcgataattc
    16081 gatcgatgaa gtattagcag gttttgcaac gaaaatccat gtgattattg aaaaagacaa
    16141 cagtatcacc gttattgacg acggtcgggg gatacctgta gatatccagg caaaaactgg
    16201 acgtcctgca gttgaaacgg tctttactgt tttacatgct ggaggaaaat tcggcggtgg
    16261 tgggtacaaa gtttctggtg gacttcacgg agtaggatct tctgttgtta acgcgctttc
    16321 tacacagctt gatgtcaaag tatacaaaga aggaaatgtg tactatcaag aatatcgtcg
    16381 tggagctgta gtggacgact taaagattat cgaacaaacg gatcgccatg gaacaacggt
    16441 tcactttacg ccagaccctg aaatttttac agagacaact gagtttgatt tttcaaaatt
    16501 ggctacccgg atccgtgaac ttgctttctt gaaccgcggg atgcgtatat cgattgaaga
    16561 caaacgagaa gaagaaccag tcatcaatga ataccattat gacggcggga ttaaaagtta
    16621 tgtcgaatac ttgaatgcaa acaaaaccgt gatttttcca gaacctgtat atttggaagg
    16681 tgaacagcaa gatatcgcag ttgaagtctc tatgcaatat acagacggct accattccaa
    16741 tatcatgagt tttgccaaca atatccatac gtatgaaggc ggaacacatg aatcaggatt
    16801 caagactgct ttaactcgtg tgataaatga ttatgcccga aaacaaaagc tgatgaaaga
    16861 aaatgacgac aacttgaccg gtgaagacgt gcgagaagga ttgacagctg ttatttctat
    16921 caagcatcct gaccctcaat tcgaaggaca aacaaaaaca aaattgggca attccgaagt
    16981 acggacagtc actgatcggt tatttagcga acatttcatg aaattcttgc tggaaaaccc
    17041 aagtgtgggc cgtcaaattg ttgaaaaagg attactagct tccagagctc gtctagcagc
    17101 gaaacgtgcg cgtgaagtaa cgagacgtaa aggtgcttta gagattagta atctgccagg
    17161 aaaattagcg gactgctcta gcaacgatcc tgaaaaatgc gaattgttca tcgtcgaagg
    17221 agattcggcc ggcggatcag ctaagcaagg tcgtaatcga gagtttcaag ccatcttgcc
    17281 tattcgtggg aaaatcttga atgttgaaaa agcaagcatg gacaagatct tggcaaatga
    17341 agagatccgg tctttattta ctgctatggg tacagggttt ggagaagatt ttgatgtatc
    17401 gaaagctcgc taccacaaat tagttatcat gactgatgcc gatgtcgacg gcgcgcatat
    17461 ccgtacattg ctgctgactc ttttttatcg ttacatgcgt cctattgttg aagctggata
    17521 cgtctatatt gctcagccac ctttatacgg ggtaaaacaa gggaagaata ttacgtatgt
    17581 acaaccagga aaaaatgcag aagaagagtt gaaacaagtc gttgcttcac ttcctgcctc
    17641 accaaaacca agcgttcagc gttataaagg acttggagaa atggatgatc atcaactatg
    17701 ggaaacaact atggacccaa gcaatcggat gatggcccgc gtcagtgtgg acgatgctat
    17761 tgcagcagat cagatatttg aaatgttgat gggggatcga gtagaacccc gtcgtgcatt
    17821 catcgaggaa aatgcacact atgtgaagaa cttagatatt taatcatgaa ggaggaaaaa
    17881 gaatgagtga agaaatcaga gaaaacatcc atgatgtcaa tctaaccagc gaaatgaaag
    17941 actccttcat cgattatgcg atgagtgtta ttgtcgcacg tgcacttcct gatgtcagag
    18001 atggtctgaa acccgttcat cgccgtattt tatacggcat gaacgaatta ggtgtaacac
    18061 cggataaagc ccataaaaaa tctgccagaa tcgtcggaga tgtcatgggt aaataccatc
    18121 ctcatggaga ctatgcgatc tatgagtcaa tggtacggat ggcacagcca ttcagctacc
    18181 gttatatgct agtggatggt catggaaact ttggttctgt tgacggtgac ggtgccgccg
    18241 ctatgcgtta taccgaagca cggatgagca aaatcgctac agaaatgctg cgtgatatca
    18301 ataagaacac tgttgatttc caaagcaact atgatgatac agaaaaagaa ccagttgtac
    18361 tacctgctcg cttcccgaat cttttagtga acggaacaac ggggatcgcc gtaggtatgg
    18421 ccacgaatat cccacctcat aacttgtctg aggtagtagc ggctatcgat ttgctgatgg
    18481 ataatcctga tgtgacgacc aatgaattga tggaagtatt acctggacca gattttccaa
    18541 caggtggttt ggttatggga aaatcaggaa tccgaagagc gtatgaaaca ggtaagggtt
    18601 ctattaccgt acgtgcaaaa gttgagatta ctgagatgcc aaatggtaaa gaacggatca
    18661 tcgtgacaga attgccttat atggtcaata aagcaaaatt gatcgaacgg atttctgaac
    18721 tgcacagaga caagcgtatc gaagggatca cagatttgcg ggacgaatct tcccgtgaag
    18781 gtatgcggat cgtcattgac atccgtcgtg acgccagcgc atctgttatc ttgaataact
    18841 tatataaatt gacatccttg caaaattcat ttggattcaa tatgttggcg atcgaaaaag
    18901 gtgtgccgaa agttttgagc ttgaaacaaa ttcttgaaaa ctatatcgaa catcagcgcg
    18961 aagtcatcac aagacggaca gtctttgaaa aagaaaaagc agaagcaagg gcacacattt
    19021 tagaaggttt gcgtattgct ttggatcata tcgatgaaat cattgcgatt atccgcgggt
    19081 ctaaatcaga tgacgaagca aaagcgacga tgatcgaacg gttcgattta tccgatcgcc
    19141 agtctcaagc tattttagat atgcgtttac gccgattgac cggtttggaa cgggaaaaaa
    19201 tcgaaaatga ataccaagaa ctcttaaaac tgattgaaga tttaactgac attctggcaa
    19261 gaccagaacg tgttacagaa atcatcaaga atgaattagc agacttgaat caacgatttg
    19321 gtgataaacg acgcacagaa ttactagtcg gtgaggtctt aagtctggaa gatgaagatt
    19381 taatcgaaga agaagaaatc gttattacat tgacaaacaa tggttacatc aaacgtttgg
    19441 caaataatga attcagagct caacgaaggg gtggacgtgg tgtccaagga atgggtatcc
    19501 acaatgacga cttcgtcaaa aatctggttt cttgttctac ccatgataca ttgttattct
    19561 ttacgaataa cggaaaagtg tatcgggcaa aaggatacga aattccagaa tacgggcgca
    19621 cagccaaagg aataccaatt atcaatttac taggtatcga ttcgagtgaa agcatccagg
    19681 caatcattgc ggtgacggga gaagcagaag aaggccacta tctcttcttt acgacgcgtc
    19741 aaggtacagt aaaacgtact tctgtcactg cttttgctaa catccgaagt aatggtctga
    19801 tcgcaattgg tttgaaagaa gacgatgagc tcgtcaatgt gctactgaca gatggtcaat
    19861 ctaatatcat tattggaaca cataacggct actctgtcac tttcaaagaa gaagcagtcc
    19921 gcgatatggg acgtacagct tccggggtta gaggaatacg cctgcgtgag gaagattatg
    19981 tcgttggggc agccttaatg aaggaagatc aagaagtatt agtgatcaca gaaaaaggtt
    20041 acggaaaacg gacaaaagta tcggaatatc cagtgaaagg ccgcggaggt aaagggatca
    20101 aaacggcgaa catcactgaa aaaaatggtc cactagctgg attgaccact gtttctggag
    20161 atgaagacat catgctgatt acagataaag gtgtcattat ccgtttcaac gtatcgactg
    20221 tttctcaaac tggacgttca acattaggcg tacgtttgat gaagatggaa gctgatacga
    20281 aagttgtcac gatggcagca gtcgaaccag aagctgcaga acaaacagtt gaagaacaag
    20341 gatcagaaga atagccatca agactaattg tgtaagctag caagaatgac cgattaaaaa
    20401 atctggttat tcctgctggc ttttttgatt agttgaattt ctcttaaaat attcgaaact
    20461 gttcattctg tattgctttt gaccaaaata gagtgtataa tcagagcttg tgagtagtct
    20521 aattaattag ataactctcc ttgctcttag gaacctaaga gccgaaagtc cacagggagg
    20581 tgaatatcaa tggaaaacac gaaatatgaa atcatgtaca ttattcgtcc aaacattgat
    20641 gaagaagcaa aaactgcttt aatcgaacgt ttcgatgcaa tcttgaaaga taacggagca
    20701 gaagttatcg aatcaaaaga ttgggaaaaa cgccgtttcg cttatgaaat gaacggttac
    20761 cgtgaaggta tctatcacat cgttaaagta tcttctccat catcagcaaa tgctgtcaat
    20821 gaatttgacc gtcttgctaa aatcaacgac gatatcattc gtcacatgat tgttaaagaa
    20881 gaagcttaat tgttgtttca tgtgaaacaa tagaaaacga tgagaggaga tgactatttt
    20941 gattaataat gttgtattag ttggacgtct gacgaaagat ccagatttgc gttacacagc
    21001 aagtggtacg gcagtcgcaa catttacttt ggctgtgaat cgtaatttta caaaccaaag
    21061 cggcaatcgc gaggctgatt ttatcaactg tgtgatttgg cgcaaatcag cagaaacttt
    21121 agcaaactat gctcgtaaag gaacactttt aggtgttact ggacgtatcc aaactcgttc
    21181 ttatgataat cagcaaggac aacgtgtata cgtgacagaa gtagtagctg acaacttcca
    21241 attgctggaa agtcgttctg catctgaaaa tcgccaacaa agcggcggat tccaaagttc
    21301 tggtcaatca gctggcggat ttggcggaaa caataactct aatcaaacat ctcaatcatc
    21361 caacggtatg cctgatttcg accgtgatac ttctgaccca ttcggctcaa gttcaacgat
    21421 cgacatttca gacgatgatt taccattcta aacgaatttg ataggaggga aatagaatgg
    21481 cacaacaaag aagaggcgga cgtaaacgtc gtaaagtaga ctacattgct gctaaccata
    21541 ttgaatatat cgattataaa gatatcgaat tactaaaaag atttatttca gaacgcggta
    21601 aaatcttgcc acgtcgtgta actggtacag gcgctaaaaa ccaacgtaaa attactgttg
    21661 caatcaaacg cgctcgtatc atgggcttgc tacctttcgt tagcgaagac taataaatcc
    21721 taggaattga atgactgatc cttttgatca gctattcaat tccttttttt ctgtaaaaat
    21781 aacctctatt cgtttagtcg ctcgaatgac gaaagttatg gtaaaatcaa attagtcata
    21841 tatgggagga aaattatgga aaataaaaac aatcatttat ccccgaaacc tctcttcctt
    21901 atcctgttta tattaatcga agtattggta gtcttaattt taccatttcg ttttgttagc
    21961 gcttcggtca ttctattgat gaatttatgg ggtgttcgct attggttgaa aatgaaacgt
    22021 tggcttattt taaataaacg tgaaaaaatc cagcaggctt cgactgtagc tgaaaccaat
    22081 ctagcttatg tctcagaagt catgcctgta ggagtgattg cttatcagcc atcaagccaa
    22141 aaaatcgaat ggctcaatcc attagcattg aaaatcaaag atcaaagtgg attaacagag
    22201 gaagaattgc tagatacttt ttttactgca aaagaaaaaa agaaaaacca agtctatcta
    22261 gcagataaaa catttcagtt tgatatggat acagtaaagg gtgttgttta ttttaatgat
    22321 attacccaaa accagttgtt aaaactccat cagacgaaca gtcagccggt gattggtatt
    22381 ttatctattg ataactatgc agatgccatt gataaattgg atgacaaaga gatttcttat
    22441 ttgaactctt tcgtcactac actagtttct gactggatga atgaatacca tgtctttttc
    22501 aaaagaatca acgcagaacg gtttttcttc gtagcacaga tgatcgatct gaaaaagatg
    22561 atggataaaa aatttgattt gctggatcgc tttcgagcag aagctgaaaa acagagctcg
    22621 cctatcaccc ttagtatggg gattgcttat ggctcaagaa ccctcgatga aatcggtgga
    22681 gaagctcaga ataatttaga tgttgcttta gtccgtggag gcgaccaagt tgtcgtgaaa
    22741 gaggtaaaag aaggagcaaa acctgtttat tatgggggga aatctgctaa gaatgtcaaa
    22801 cgcacgcgtg ttcgttctcg agcgatgagt accgctttgc gtcggatttt tgatgaaaca
    22861 ggcgatgtgt ttatcatggg gcacaagttt ccggatatgg atgctttagg ctctgctttt
    22921 ggtgtcagtt gtctggcacg cttcaaccag aaaaaagctt acatcgttat caacgagaac
    22981 gagatcattc cagatgtaga acggtgtctt gaagaaatcc ataagcatcc tgaattagaa
    23041 atgcaactaa tctctccaga acgtgccata gagctgaaaa aagaagagga cctcctcgtg
    23101 atggtggatt accataaacc ttctctatcg atctctcagg gcctatatga gcaatttgat
    23161 aagattgtga tcattgatca ccatcgtcgt ggggaagagt ttccaagtaa acctctgctg
    23221 acttatatcg aatcttcagc ttcttcagct tcagagctag tttctgagct gatccaatac
    23281 caaagcagca aaacaaatcg cttagatcgt tttgaggcca cattattgct tgcagggatg
    23341 gttgtagata caaatagttt caatgtccgt acaactgcac gaacttttga cgttgccagc
    23401 tatttaaggt cttgtggagc agacgcatcc ttagtacaat atttattgag ctcggattta
    23461 gcgacatact tggagatcag caatttgatt gctggaagcg actatgtgac ttctgatatt
    23521 gttgtggcgg cagggacaga cgaaaaagag tataatagtg ttatagcagc aaaaacagca
    23581 gatacattgc tttcgatgat caatatcaat gcagctttcg tcattacaaa gcggacagac
    23641 ggcttgattg ggatcagtgc cagaagtacc ggcacgatca acgtccaaat catcatggaa
    23701 tccttaggtg gcggaggaca cttcacaaac gcagctactc aactggaaaa tacgacagtt
    23761 gaggaaacaa aacagaagct atttgctgca atcaaagaaa atatgaatca aatatatgat
    23821 aaggagtaat ccaaatgaaa gtaatttttt tacaagatgt aaaaggaaaa ggtaaaaaag
    23881 gggacatcaa agaagttcca gcaggttatg cacaaaatta tctaatcaaa aataacttgg
    23941 caaaagaagc aacgaaaagc agtatcagtc agttagcagg acaaaagaaa gcacaagaaa
    24001 aacacgaagc tgaaatcttg gaagaagcta aaaagttaaa agaattcttg gaaaaagaag
    24061 atactgtagt cgaattgaag gcgaaagcag gggaagacag ccgtttgttt ggttcgatcc
    24121 cttcaaaaca aattgctgaa gggttaaaca aacaatacaa aatcaaatta gataaacgaa
    24181 aaatcgaatt agaaaatccg atccgttcac taggctacac aaacgtacct gttaagcttc
    24241 atcatgaggt gacggctacg atcaaagttc atgtcgtaca agaataacaa atcttgatag
    24301 cgatgttcag ctggtaagta taaccagaag gaaaaaagac aaaccgaaat ttatctgaga
    24361 aaacagctgc agcaatgaaa gcaaaagctg caaatcgatt tcagattgat ttcggtttct
    24421 tttttgggaa cagcaaaata gcataaagaa aacgagtata ataaaagaga actatttttt
    24481 tattacactt tcggtacgaa tgatgacttt tctctcattt tcttgtaaaa tgaagcaatt
    24541 gaagacaagg agcgatacgt atgaatgaag tgtggcagga tcgaatccca ccgcaaaata
    24601 ttgaagctga acaagcggtt ttaggctctg tttttcttga cgcagaaacg atcattgatg
    24661 ccatggagta catagagcca aaagactttt atcgtcgtgg ccatcaaatt atttttgaga
    24721 caatgatcga gttaaatgat cgtaatgaag cgattgacgt ggttactgtc aaagaccggc
    24781 tggaacaagc caatttgtta gaagatgccg gtggtttaag ttatctctca gacttggcat
    24841 tagcagttcc gacagcagca aatatcgtct actatgcgaa aatcgttgaa gaaaaatcat
    24901 tgctgcgtac attgatccag acagcaacag gaatcgttac gaaaggattc gagcaaggag
    24961 aagaagttca aacaatttta gatgatgccg aacgcagtat cttggaggtt tcggaaaaaa
    25021 gaaatcgaag cggcttttta tctattgctg aagtactgaa ctccacgatt gaaaatatcg
    25081 atcgactagc tcaaaacaat gaagaaatca caggtctacc aactggctat caaggtctgg
    25141 ataaaatgac agcaggtttc cagcccgaag aactaatcat tcttgctgca cgtccagcgg
    25201 taggtaaaac agcgtttgca ttgaatattg ctcaaaatgt aggaacaaaa acagatcgtt
    25261 ctgttgcgat atttagtttg gaaatggggg cagaatctct tgtaaatcgg atgctttgtg
    25321 cagaaggttc gattgaggcc agccatttac gtacaggtca gctttcggaa gaagaatggt
    25381 cgaatctgat cgttgcgatg gggagtcttt ctcgagcaaa tatttttatc gatgatacac
    25441 cagggatcaa aatctctgaa atccgtgcga aatgtcggaa actagcgcaa gaaaaaggca
    25501 atctaggctt gatattgatc gattatttgc agttgattga aggtaacggt aaagagaacc
    25561 gtcaacaaga agtatcagat atctctcgcc aactgaaaaa actggcgaaa gagctgaaag
    25621 tacctgtcat tgctctttca cagttatctc gtggagtaga acagcgacaa gacaaacgac
    25681 cagtgctaag tgatattcgt gaatccggat caattgaaca agatgcggac atcgtagcct
    25741 tcttgtatcg agatgattat tatgaaagag aaggcggaga tgacgatgat ggaaacgagc
    25801 caaaaaatga caacgtcatc gaagtcatca tcgagaagaa ccgaagcggt gcacgaggaa
    25861 cagttgaact actgtttatc aaagaataca ataagttttc gtcgctttcg ccaagagaag
    25921 aattttaaag aagaggttgt gagccttgag caatcccgag agctctcgta agaagatgca
    25981 aggagtaacc gcaggaacga ctagagtact taatttaaac agatctccaa agggattgtg
    26041 acaaaagtct tgtcacagtc cctttttccg aataaacggt gttcaatcag ctgacccttg
    26101 gtattagctc agcaaacgtc aaaatatgag gaactatttt gacgtttgtt tctctaatac
    26161 tcaggtcaaa acgctgatct tacgacctct tttccttaaa cggtgttctc ctgccaactc
    26221 ctcggtcaca agtcacaata tcatcaaaca tgagaagcag ttttttgaaa ttgctccttg
    26281 tgactcagag ttaaacggca ggctcacaac ctctttctta aacggtgttc aaaagcttgc
    26341 atctgcggta atacgcccaa tcaaacgaaa aatatgag
//
LOCUS       DACEPD010000032        26351 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.032, whole genome shotgun sequence.
ACCESSION   DACEPD010000032 DACEPD010000000
VERSION     DACEPD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.032"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(97..1227)
                     /locus_tag="IW797_001280"
     CDS             complement(97..1227)
                     /locus_tag="IW797_001280"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ1224065.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1876..3078)
                     /locus_tag="IW797_001281"
     CDS             complement(1876..3078)
                     /locus_tag="IW797_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1224066.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3138..3341)
                     /locus_tag="IW797_001282"
     CDS             complement(3138..3341)
                     /locus_tag="IW797_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ1224067.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3483..4715)
                     /locus_tag="IW797_001283"
                     /pseudo
     CDS             complement(3483..4715)
                     /locus_tag="IW797_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4878..5690
                     /locus_tag="IW797_001284"
     CDS             4878..5690
                     /locus_tag="IW797_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1224068.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6714..6866)
                     /locus_tag="IW797_001285"
     CDS             complement(6714..6866)
                     /locus_tag="IW797_001285"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224069.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7057..7935)
                     /locus_tag="IW797_001286"
     CDS             complement(7057..7935)
                     /locus_tag="IW797_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1224070.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7974..9092)
                     /locus_tag="IW797_001287"
     CDS             complement(7974..9092)
                     /locus_tag="IW797_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ1224071.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9089..11188)
                     /locus_tag="IW797_001288"
     CDS             complement(9089..11188)
                     /locus_tag="IW797_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ1224072.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11239..12060)
                     /locus_tag="IW797_001289"
     CDS             complement(11239..12060)
                     /locus_tag="IW797_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1224073.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12057..12866)
                     /locus_tag="IW797_001290"
     CDS             complement(12057..12866)
                     /locus_tag="IW797_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1224074.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12887..13381)
                     /locus_tag="IW797_001291"
     CDS             complement(12887..13381)
                     /locus_tag="IW797_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1224075.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13393..13881)
                     /locus_tag="IW797_001292"
     CDS             complement(13393..13881)
                     /locus_tag="IW797_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1224076.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13958..16654)
                     /locus_tag="IW797_001293"
     CDS             complement(13958..16654)
                     /locus_tag="IW797_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1224077.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17082..18155)
                     /locus_tag="IW797_001294"
     CDS             complement(17082..18155)
                     /locus_tag="IW797_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1224078.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18152..18979)
                     /locus_tag="IW797_001295"
     CDS             complement(18152..18979)
                     /locus_tag="IW797_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1224079.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18980..19786)
                     /locus_tag="IW797_001296"
     CDS             complement(18980..19786)
                     /locus_tag="IW797_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1224080.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19798..20883)
                     /locus_tag="IW797_001297"
     CDS             complement(19798..20883)
                     /locus_tag="IW797_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224081.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20900..21442)
                     /locus_tag="IW797_001298"
     CDS             complement(20900..21442)
                     /locus_tag="IW797_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1224082.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21700..22689
                     /locus_tag="IW797_001299"
     CDS             21700..22689
                     /locus_tag="IW797_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ1224083.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22686..23423
                     /locus_tag="IW797_001300"
     CDS             22686..23423
                     /locus_tag="IW797_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ1224084.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23420..23971
                     /gene="coaC"
                     /locus_tag="IW797_001301"
     CDS             23420..23971
                     /gene="coaC"
                     /locus_tag="IW797_001301"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ1224085.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23987..24619
                     /locus_tag="IW797_001302"
     CDS             23987..24619
                     /locus_tag="IW797_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1224086.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24704..25351
                     /locus_tag="IW797_001303"
     CDS             24704..25351
                     /locus_tag="IW797_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1224087.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25356..26339
                     /locus_tag="IW797_001304"
     CDS             25356..26339
                     /locus_tag="IW797_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ1224088.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8060 a         5570 c         4465 g         8256 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt gccaagcctc acatttttat agccaaactt cttttgcgac
      121 ttgctgcaat aattggattt tcttccattg ttcttcttct gttaaatggt ttccttcttc
      181 tgttgaagca aacccacatt gaggactgat acttagattt tccaatggta caaattgttg
      241 tgcttctttt attcgttctt ttattttctc cgtatcttcc aaatctggaa acttagaagt
      301 aactaatcct aaaacaactt tactttcttt gttactccaa tgtcttaaag gagtaaaatc
      361 tcctgctcgg tcagaatcgt attccaaaaa gaaaccatca taatttgttg aaaacaactc
      421 ttctgcaatg ggttcatatc caccggaaaa cagccaagaa gaagcgtgat tcccgcggca
      481 gatatgtgtt gtgatcgtca aatcttctgg tttgtcttcc agtgcgttat tgataatctc
      541 tgtacaaata tgggccattt ctttcggatc aattcctttt tgaagaagtg cttctctttt
      601 agtttgatca gctagaaacg cccaatttgt atcatcgaat tgcaggtaac ggcaaccttt
      661 ttcataaaaa gcatgaatcg ctgctttgta agtagaggac aagtcctcat aaaaatcaga
      721 tagatcagga taaatctcct tgatttttga agaaccatca ttagatagga tctcttgtcg
      781 taaaatcatc gtgggactag ggatcgttgc ttttgcaaca aaagtatcat ctgtttccac
      841 tacttctttc agaaaagcaa aatcttttaa aaatgggtga tttggattga aagataattt
      901 atctactacc cgaatattat aagctggtgc tgcttttccg tggaatgttt gattatatcc
      961 agtttctgga acatagcctt cgataccatt tagttcttct aaaaaatcaa gatgccaaaa
     1021 tccacgccga aattcaccat ctgtaattcc tttgatgccg actgcttttt ggtcttccac
     1081 taattttttt atttcctggt cttcgattgc acgaagttct tcttttgaaa gtaagccttc
     1141 ttgaactttt tttcttgctt ctttgatcga ttgcgggcgt aagaaacttc cgacatgatc
     1201 tactgtaaat ggtctatttt tgctcatttc actcactctt ttctttgttt tttgtaaaaa
     1261 aaaatccttt tactagcaaa tactagtaaa aggacgatta tactcgtggt accacctttt
     1321 ttcagaaaaa acattctttc tctcagcctg acaataaaat cagtggtctt ggtaacgaaa
     1381 gactgttcgt ttttgcctac ctttcgacaa aaaatcctct aaggccattt tcgacagtca
     1441 acttctagct ttttacacca accaaagcct ctcttgaacc attttctgtc tactttcctt
     1501 ctcatcggat actattagaa taaaattaga atagcatgtt tgaaagaaaa gtcaataggg
     1561 aataagaaca aacaaaaact aatttgttat tcttagtaat ttgtttgtgg aatacaataa
     1621 agcttttcta atatgataat tgttcagtcg tcctttcatc ttagatcact tccttaaaat
     1681 gatcataccc gtttcaacca cataaaaaag gacttaattt tgaaaaattt cccatcaaaa
     1741 ctaagtcctt tttattatag ttatgcaaat ttgcttattt tctcgctatt tccgatacat
     1801 cttgaacagg agcaattcga ctgaaaacac tggtgcaaaa gcatttattt gtcttatggt
     1861 agaaccgtgg aaaactcaga ctggtaataa attgtcattt tttgaaagaa tagcgccctc
     1921 ttgaacatta tcaatcgcac ttaccacttg ttgatctgac tttgcatata agtgaccgta
     1981 agttcctaaa gtgatttgaa tattcgcatg gcccaatcgt tcttgaatag atagcggttg
     2041 aatattctgc tcaattagaa atgcaacatg agaatgtcgt agcgcgtgaa gcttaatcgg
     2101 tttgacaccc gcttttttag cagcttcttt taaccagttc gcaaaagaat aaggtggcgt
     2161 aaatgttccg tccaactgag caacatattc aaaatttcca atacgttttt gcaagtcata
     2221 ccactgtctg agttttaagg aaagcttctt ccccaaccca atcgttcgat aagcacattt
     2281 agttttggga gtcgaaaagt accaatcatc ttttgtatta taaatcaatg tcttctcaat
     2341 cgttatcgtg tttttctcaa aatctacatt cgaccaagac aaagcttcaa gttctccaat
     2401 ccgaatgcca gtataaaaga gcatttcata cacaatcttc cgatggtggg tttgaatctt
     2461 atcatccttc aattcattca tcactttttg aaactcactg tgttcccaaa actcaacttt
     2521 cttattctca gaaggaagcg aagaaacatc ttcaacggga tttttagcaa tcaccccctc
     2581 tttaacagcc cgatccaata caatttttag gtgccctcga atccgattga tataagattt
     2641 tgataacgtt tcgccattaa cttttttccg tgaattacgt gactctgtct ggcttagttc
     2701 aaataaccaa tcctcaatat cgtgacttgt aatatctcga acacgaaaat gatagaaaaa
     2761 ctgcatacgc ttttcgaaaa tgaattgcgc attctcatat gtttgacttt tgacctgtct
     2821 ctcgtaccat tttacaaacc tatccttgta aaactcttcg aaagtcattt gattggtaga
     2881 acacagttga ttaggaattg tcttgttaaa atcatgtgtt tccatgtact tgtcataagc
     2941 aattttggca tctttttgtg aagcataacc tcgtttagtc ttctgaacct ttcttccatt
     3001 ttcatctata cctagatacg ctcggaagaa ccattttttt gtctttttat cttggtaaat
     3061 atttttaatt tttggcatcg ttccattcct ccctgtactt gagaaaagct actttaatag
     3121 taccgcgctt gtactgttta ttccgcaact tgaatgtgaa aaagatgttc aattactcgg
     3181 gatggcacca caccaacttg gcgattatta taaaaatcaa taccttcaat ccttgcaaga
     3241 tatgccttgc tctctttaat cattcgtgca gcttgactag gtttaaagcc catctctatc
     3301 aaaattgcac gatttaccat gaaatcacat tttgtttcca ttctctcacc tcctgattct
     3361 ttactccttt taaaataatc ctctttataa tctattgttt ctcattcgat ggttgtgtta
     3421 tactcgtctt gtcgaggcgg agtacgttgc acaacttgtt gtgtaagcag tactttgctt
     3481 ttttagtagc ttgttttctc atttgatata ctttgtaatt ttcgttcata atactcaacc
     3541 tcctcctgat aatactccgg gtctgccttc atattttgga gaagtttgat ctgtcttgga
     3601 ttcatttctc cttgttcaat aatcaaaggc aacaaggaag aatgaaacgt ttgatcgacc
     3661 tgtgcaataa aaactaatga tggtgaaaca ccatgaatta accaactcat agttttctca
     3721 aaactttgag gaattgtatc taaagaaatg gtgattgcgc cataattgct aataaaggct
     3781 gcccatttgg catcaacttt tgcacctaga tcatttggat cacggtcata aaaacaaatt
     3841 gctccattaa gtaactcaaa gaacagtttg gttaaatcat gggtttttga aataatcttt
     3901 gccaagcttt gcgctttctc ttttcgatac cgtagttcaa agcgattctt tacttttgcc
     3961 tcttctaaag gaattcctcg tcgcttccgt tgttcatggt ccttctgata aaagcaaaaa
     4021 cgacactgac tcttctttga gcctaaatac aaggtctcac catttgattc attgtttatg
     4081 atatccgtac cgccatgaga ttcactcact tgaaaagtgg tccacacatg gcctagtatt
     4141 accttatgct taagttctgg aatgctgaga ctccccacaa aatcatctaa agtaaaatca
     4201 atccgtgtaa agttcccctg ataatctaat accttacgaa aaaactgttg ccaatttgtt
     4261 tttcgtgttt ttagccatct tgctaagtgt ttacagcctt gacctgaaaa ctccatcaca
     4321 gttcctttaa ccgcatcgtc aatggaatac cgaatcgtga ttacttgatt ccacacgtat
     4381 tggccaatat agccctttgg cgcataatcc agttcctcaa aacgttcaat gggcatttca
     4441 ataatctctt caattagctt taaatgttga ttcgagaaaa agtgaatggt tagataatca
     4501 atcaaaacac ggttaactgg aacgacctct tcttgcaaac tatccaacac aagattcaat
     4561 ttttcttcac ttaattgtct cgttcctttt tctactttcg ataataacta tctcgaaatc
     4621 cccacttttt ctgctaaata tcgttgtgtc cacccccttt cttctcgaga aactcgaatg
     4681 gcttcaccga gatcatgttg attaacttgg ttcatcgaca aacctccttc tgctatttac
     4741 ttgtcaaaga acctattgaa cttattttaa gtacattttt attgtacgcc tgtatttatt
     4801 ttaagtcaat atgttttgac tttttttaag tacacacgta tactaaattt aagaacttat
     4861 cctgaggagg agttttcatg gagatagaca agcaagcagt cggcaatcga attcggcaga
     4921 ttcgccaaga attaaagttg agtatggaga aatttggcaa actaattggt gatttaccgc
     4981 gaagtacagt caataactgg gaacgtggga tcaaccttcc aaaaaccgaa acccttcacc
     5041 aaatcgcaga gataggtcat gtcaccaatg aatatctttt gtatggtgat caagagaatc
     5101 aatacatttt agaaatgctc caaaaaaagg caggtaagct tgatcctaag attgaaggtc
     5161 tcatagtaga cgaaatcaaa cacgctgacc ttgttagtga aaaagaaatg gatcgaatga
     5221 ttgaattttt tattacaaat ctcattccac caactgaaca agaccagttt actttccaat
     5281 gtatcgatga aaaaaagcag ctttatcttg gttcaactaa ttttggtaag gaagcccagc
     5341 tctatttgca acatgataat caaaatcata ttctgcacat gatgcccttt accttctcca
     5401 actttagtgt ggatcgcata ctcgtctatt tagcaaatca aaaatcctat tcctattttg
     5461 ggaaacattt acccaaggaa ctaggtgaga aagccatcct tctatactca attaatcaat
     5521 tgaccagcga tgtgcgaatt gccccgttgg tctattcaga agaaaccgct tcttatcagt
     5581 acacagagga caatcaatat ctactagaac agcaatatct ttatctacca ttcgtgatgg
     5641 aagtcgagaa agaacgcctg ttaaatgcag cgtatccatc atcaaaatga ggtgacaaaa
     5701 ttgataagca cgtgacaaag aaaatactgc taggacactc cccaatgggt agttgtcaca
     5761 gtttcctgtt ctttgttagc cccctgttag aaccgggggc tttattcctg cagtccggct
     5821 acgccttcct gcaatagaca aaactccaga tcgggtaatt cgcttgtggc gaaacccgac
     5881 ctggagtttg tctctgtatt ttgaacccta aaaattaatc aaacagactg atattctcat
     5941 aatcagcgcg aaataagcca ttaagtacag ttttcctacc tatttttgat aacccgctgg
     6001 ttagttaata ttccttttcc gagagagata aatgattggt taaatctata tacatctcaa
     6061 cctttaaaaa tgttttcgaa atacttcatc agtacatgtc atacaaatta ccagtgcatt
     6121 ctcaaattct agttctttaa ttgatttcag ttgaggagca gttacttttg gcggaaattt
     6181 ccatttttgt ccaaacctct tcccccgatg aaatttcttt gttgcaatta ttgcaatgaa
     6241 aaatttttgt ttcaaaaata tacataatta cttcctccaa taaaaactat ctacctctca
     6301 aattattttt ctctaataag aaaaacagaa aaggtaataa gatacaacag tgatgattcc
     6361 taataaagaa aagactacaa taatattttt ctaacttatc gccttcaaag aaatagtttc
     6421 ttatttttca ctaaaataaa aaatttattg caaaaaaaat agaagtgaca aaattatcaa
     6481 tcatttcaag gtaatataac tacctgagtt tattatcgat taaaatgcta actacatttg
     6541 caagagaagc gagagttaaa ggtaaatata gaatttctac ttttttagca atagaaatag
     6601 gaataaaata tttaaaatgt atagaaactt tctttttttt tcttgctatc tttttataat
     6661 cacaactctc tataatagaa ttacgattta ttttatacgg ctgaaaaaat ctatcaccaa
     6721 aaaaattcaa acattaagcc aaggattatg aagtcaacat ttcgaaaaat gaacatacat
     6781 tggtaagcaa ctataactat taccacagac ataaagtaac acaatatatt actatttaag
     6841 tgtacgccac cagtgaatat cctcaaggta tacagcgcac aacaaaaaga aaaaaggaaa
     6901 cgcagaagca tttcctttca gtgatttaat gaaaacaaca cgatttgact tagagccttt
     6961 ttatttgaat tcattttcgc tttatccaac ttcatttgat catttgatta ctcggttaaa
     7021 tacttttgac atgagtatca atgaaacatt cattaattat ggttgcttat ttcttaccgc
     7081 ttttctggcc aatgctaatc cgccgattat tccggcatta tcacctaatt cgggcgtaat
     7141 aatgtagcta tcaattggtg gtatttcaac atatccattt ataagctcaa caaatttatc
     7201 ctgaactttt ttcttcatgt gttcttgttt cattacacca ccgccaaaaa taattctatc
     7261 tggcgaaaac atcagtgttg tattgtatgc acattgggca aggtaaaagg cttcaatctg
     7321 ccaaaacgaa tcgtcagcca ataaattctg gccttttaca cccaaccgtt tttcaatcgc
     7381 tggtcctgct gccatcccct ctaaacaatc ttggtggaag ggacagtttc ctgaaaaatt
     7441 gtcctttgga tgtcgcttga tcaacatatg ccccatttcc ggatgactga acccttccac
     7501 gaaagtccca ttgaacaagg caccacctcc gatacctgtt cccacagtga agtatgcaac
     7561 acctgctaac cctttgccag aaccagcaac atattcacca tagcaagccg cattgacatc
     7621 tgttgtccat tcaatcggaa tattgaaata ttgctttagc gttcctacaa aattgaaatt
     7681 ttgccaagca agttttggtg ttgatgtgat atgaccgaaa ctcgttgatt tgcattgaat
     7741 atcaattgga ccaaagcagc cgaccccgat gcctgccaat tgttccttaa aaggtttaaa
     7801 aaaatcaatg acctgcttca ttgtttcaac tggagttgtt gtgggaaaac tgactctttc
     7861 caaaatcgtt aattcctcag taccgactgc acagacaaat ttagtccctc cagcttcaat
     7921 tgccccatat atcatcatag gttcctccct tcgaatatgt gaaaaacact tcattaacgc
     7981 ggcaagcgtt ctcgatcagt gaaatgacgt ttagtatttc ctgaagcttc tgttgaatac
     8041 cagtaagcga cactagcaac atcgtcttgc cgctcaaata agcgaacatc atcgttgccg
     8101 atttgttgta aagtgaccga taaatcttcg gtaaaacaga tggggtcttg aagatgccaa
     8161 cgataaagac catgtcgtgg cattgcgtcg tcaccaaagg catgaacggc attcaatttt
     8221 cccgtttcaa aacgatctct tgtggcatct ctatttgatt ggtaaggata ccctaaaaag
     8281 gctgttgaat agtttttcgc atgtggacgt ccgaactcat cgtactcatg aaaggcccac
     8341 gccccgccaa aataatcttc tgaacctgtt gaggttacgg tcggatactc actatcccca
     8401 tcaatgtaga atttaaactc gccttcgccc caccaataac gttctaggca ggtaagcgca
     8461 aggtaagtac ccacgtaata gccttgaccc tttaatcgat caattaactt ataatcctcc
     8521 tgtaatttgg taatgcgttc ccgatgccaa tttgcgtgaa aatataggct attttcttcc
     8581 aattcgtcca ccaaagcgta atttatcgta taaaaaaatg aggacatatc cttcggatgt
     8641 aaattctcga tcgtaatttt agcggatgtt tggaaaggca tttcgaaata ggaattaaag
     8701 ccccctgtag gattaaccac tatcggcaac gagttcacct cgcaacgctc tccaaaccca
     8761 ttacaaaaga aatcccctaa aggactttca acagcagggc tatccgaccc atcccaataa
     8821 attcttaaga ttaaatcacg taaaacaaaa ctgccaaatt ctgttcgatc acgaatcgtc
     8881 atccacatat gacgaattat tcccgttcca gtaatatccg caatcacagt ttcttcacca
     8941 gaagtcagcg gaattgcgcc acgacctttg cggcttggtc ctagctcgct tggcaacatc
     9001 gcagctttac ctttctcgcc tgttggattt tcgggagtga ttgctcgact ctggattttt
     9061 ttaaattggg taatattttt tagtatactc atctttttct cctttgaatg tgaatcgttt
     9121 ttcccttttc tacgtgaaga atttttttcg tatcacttat tttcaatggc ttttctgaca
     9181 aatttgaaat actcaactcg gattgcgtac attcaatcga aatgaaggct tgaccgaact
     9241 tcaaattctt ccaagaaaac gttaaattat caacaggatc aatttgcaat actccttttt
     9301 tgaatttcaa tccgaaatat tgactgatca ttaggctgac aaaaagaccc gatgcccagc
     9361 cgcatttgcc gcagccaaag tctcgcacga cttctccttg ttgagcctct aagcgatacg
     9421 gccaccacca ccacgaaccg tctaaatctg ccaaacaaat tagttcttct aatcgggtta
     9481 cgaaagcttg agggttgtgc agctcgctca ttaaaacagt gataaagcca ggaaaagtgg
     9541 cgccggattc taatccccaa gtgatgcctt tgatttcctc gctataggtt ggattgaaag
     9601 aactagcaga aaaggccatg gtgttctgat ataaatgatc tttattatct aaaaattgat
     9661 aaaaaggcat caacgtagtg tctgattctt ctccatcatg catcaataaa ttaatttttc
     9721 catccacaat cacatcactt aaaaagatta acccttgctc aagaatcggt ttttgatagt
     9781 gcttcactga ataggttgtt ttctcctcat caccgattcc ctccaaaaat tgctcaccaa
     9841 aaggaccatc gatagtcatt tgtttacaaa ttgattcctt tagtttacag gcaatgtttt
     9901 tgtatcgctt gctaagactc agttgttcta gggcttcaaa aatttctgca agtccaatac
     9961 aggccttcca aagacaaaga ttgctgccgg tatgatactt tccaagtgca aaggcgtctg
    10021 ataaccaaac agatggaaaa agcattggtt cccctgaccg acgttgagct aatatgtcct
    10081 caataattct tgaagcattc agtagaacct gcggacaccg ctcaaagaat tttgtatccc
    10141 cagtatgttc aaaataaaga ctagccagta acgcactgga taacgaatta ctcagagaat
    10201 gattgatacc tcctgtaaat tttgaaccag gaaacttaac gccatattga tcaaaccata
    10261 gaaccgtttt ttgacataac tccggttcaa acatggtgaa tggcaatgat gcgtaataca
    10321 tatccttatt ccaaattcga ttagtagcag gatagctgcc ccaattgctg ccgacgacct
    10381 gtccctgtcg attcataccg aaactagaaa agctttgata aagtgcacga cgatagagat
    10441 ggacaactct atcgtttggc attgacagct gcccaaatag ctgagcaaaa tagttcaatg
    10501 tttccttcag ccatttttcg atatcagatt cctgaaaagt cattacttct tcaaaagcat
    10561 ttgggtccac aaaagcaatg gcgaagcttg ccacttcctg ttgtgcgatc gtgtccttat
    10621 ttcctttttg agaaatcaac acattttgct gatccgaata cttctcttga tacgaaggca
    10681 catctttcac tgaaacttga aggctttcgt gtgtttcatt cacaatgtaa acctgttgaa
    10741 caagcatact taaacgctta ttagccagta tgggagcaaa ggatactaca atgaccttaa
    10801 acgtcgaata atgatgaata tatctaggca aacagtcttg aatcacatcg acttcaatac
    10861 gatgatccga ttctgacaaa tggtagacaa tcccgtttac ttcaaccttt aaggataacg
    10921 aattggtttg agagagctgt ttattgaccc aaacacctgg tttttcctca gttagtaaat
    10981 tttctcgata aaaggtcaac gtttgaatcg tcccgcaaac atcaaggttt gctaatgtct
    11041 tttgattact tacatcgaag gtcaatccct ttacatcagg aatattccga tagtagctgt
    11101 attcgtttga agtagtcaca tttccgtcgt ttaactcagc attaaaaaat gggtgtttgg
    11161 gtgaataaat tacttcatta atagtcatct tttccctcct tgctaaaaaa actgcagcct
    11221 agtagctgca gcttcctact atgcgattcc aagtaatgac aataaaattc ccaagacgac
    11281 cattccaaaa ataattttga tgacgccgac ttttttatct agtaatttga aacagctcaa
    11341 tgttaccaat aacggtacaa ttcctttaaa aatactatcg aggatttctt ggacattcat
    11401 tagttcttcg cccttcattg aaaagctcaa aacggtattg aatttgacca tactgatcgt
    11461 cattgcacct accatgatca gacccaagat actagcagct tttgtcagaa tttcaattaa
    11521 cccgttcgca tacagctttt caatatattt agagcctaat gaatagccta aaacgccgcc
    11581 atagtatcgc atcaagtaat taggtatatt gaacataact aaaaagataa ttggtgccaa
    11641 aatatttccg gcattggcta agttgatcgc tagtcccgcg gtaacgacac gccaaactcc
    11701 ccagaacaat gaatcgccaa ttccagccaa tggtcccatc aaactcgttt tgatcgcgtt
    11761 gatactttcc acgtcaaaac cagctttttc agaattttcc ttctccatcg atgccgcaat
    11821 ccccatgaca aaggacgcgc aaggaatggt cgtcgaaaaa tactgtgtat gacgttccag
    11881 cgccgcagct cgatcttctt cacttttgta gaaatgatta atgaagggca tcatagaata
    11941 ggcgaaacca ccgccgccca tcgtcaccgg attcaccgaa gcatacatag tcatcgagcg
    12001 ccaaaacatc ttccgaacca attttttctc atctttagaa attccatcag ctaatttcat
    12061 tgaaagaaat cctcctcttc ttggtcaagc gttgcattta cagcagcatc gctttgaact
    12121 gcatgaacag atatattttt tagttgtaga tctctttggg ctataacaat tgcaataatc
    12181 gttccaactg cggcaattgc aatcaatggt aactcgagat acgccataac aacggcccca
    12241 aagaaaaacc acacagcaat atttttactc caaagcatcc gtaacagcat cgccatccca
    12301 actgccggca gcagatttcc agcgactgtt aatccgttca tgatgacctc tggaatatta
    12361 tccaagacgc ttttgacaac atctgctcca aataaaacgg caaagaatgg aataagagca
    12421 taaagcgcat agtggacaaa ccacataaag aagtgcagat tgatgattcc tttttgatca
    12481 ccagattttg caaatttgaa aaatttagca gccatcggtc cacatacaaa agaaaaaatc
    12541 atattcatta actgtaatcc taatactgct agtggaaaga caatgggaat aatcgcatcc
    12601 acgtttcctc ccattttgat tgcgaacgct gtagccagcc cagcagccaa agaaggctcc
    12661 gccgtactag agattccgat attgacgaca cccataaaga tggcttctag tgacgccccg
    12721 acaatcaacc ccgtctgcaa atcccccatc aacgctcctg ctaacggagc aacaacaatc
    12781 ggacgactca gcatggacat cccgaacatt tcatgtccag caactccaat aaaaacggcc
    12841 aaagcgacta caaatgcatc atacataatt tttctccttc ctttttctaa aaatcactca
    12901 aaatagagat agctgaacgt ttttccatcg ccggtgtagg ctgaaaaacg gtatctggat
    12961 aaagttcaac taattctttt aagtcttgaa tttctgacgt cgttaacatc acttcctttg
    13021 ataaggtttt cttatcttcc gatggcggtg tcattttccc agcattcccc acgttcatta
    13081 attcgatttc cgttgggaaa gctttcgcaa cttttaccgc atctttgaca ttagggacta
    13141 aaaccatcaa gcgcatgtcc ttcgcccgcg gatcttttaa caaatgaatc gcgtcatcaa
    13201 ctgttttaat aatgactttg acattcgatg gtgcagccat cttcaatgtc atcttttgaa
    13261 tatcgttggt agccgcctca tcgcttgcta ctactaaccc ggttaaattt agactctttg
    13321 tccaagtcat cgctacttgt ccatgaatta atcgttcatc aattcgcact gctttaatca
    13381 tttttcttcc tcctaaaaaa aatcttcctc ttcatttact acatgtacct catcaagaat
    13441 ggtcatcttt aattcctgtt ggcatttatt taccagttcc tgaatatcct cctttgttaa
    13501 ttccttggtt gccaatacta tttcgagcag aattggcaga ttaaagccag acaaaatagt
    13561 tacagcaatc tctttctcca aaaaggtttt ggtaatcttc tgattgacac ttccgccaaa
    13621 taaatcggta aaaacaaaaa tctgatcctg ctcgttcacc tggtttacaa atacattcaa
    13681 ctcctgttca aaatcacact catccacata agcattaatc accgtaatct cttgaacctt
    13741 ccccgtcagt aattgaatac tgctaagaaa gcctgaagct aagtgaccgt gagtcgctat
    13801 tagaattttc ttcatatggt taattccttt ctttctgttc acttttatat aagcaattcc
    13861 tgtgccaact tttaaaaaca ttggtttaat cacgttaatt cacattaaaa aaagtgtcta
    13921 acctaaaaaa aacagattag acactttttt agacacttta tttttgaata tacggtccta
    13981 ataattcata gagcagcgaa agctcataag cattgacttt aaaattatat ttcatctcca
    14041 ttgattgaaa aatacttcta gaaacataat aaaattcatc ttcatctggt ttcaaatcat
    14101 aattcggttc ttcagatttt tctgtccgag ccaagaataa acgttccatc atcaatgccg
    14161 tatgcatata gagatttagt tttacacggc catttaaagt gaaatgatag taattttcat
    14221 atttagaaat gactgtttcc acttcactca tcacgatcgt tgggttcaga aagttcaaac
    14281 ggtccgcaat tccttccaac gaaaagaatt ttactagctc ctgaatcaag cggttaaatt
    14341 ctgccggctt catttcagga ctcaaaatat gctccaaatt ggctgctcca tcaccgtcta
    14401 agatgtcata aacattaata ttaggaattg aaaaggaacg tggaagatcc gaagtcgtga
    14461 tgaccaattt tgtcttgtca aaataagttt catcattttg gtcgattaat tcacgtagtt
    14521 ctctgtagtc cttcgtgaaa acttcgatgg attcatccga taaatgatga cgaaaaacat
    14581 ctttcaattt gtccgaaatc cccaaacccg acatacagga aatgataatg ttgctacttt
    14641 gagaaaagcc ttcaaagtat tgaacattga ttgaatactt gtctgctgca tcctccgcaa
    14701 tctgtttaaa tggcgcattc atcgtcatct tcaagccaat atcaagtgca atggaagtcg
    14761 tcaaattatt cagaagaagt aactcccctt ttaaatcgtt cttgatctgt gtgtaaattt
    14821 gatttaaaga gcccatatct acgatcagca ctagactatc tgtattcact tgccgtttca
    14881 cgaaattcct tgtcgattcg acaatctctg acacattact ctctactggc atatccaacg
    14941 cttcaaaaac ataattgccg cataattgat tcactactgc ttgaatgctg ctagccgtac
    15001 tatctccgtg agcaagcaac aggcccttca gctcgatgga ttcatctaca taatcagata
    15061 aagtcacgac aataagcagt cttagaaagt tctcgtcctt ttctgagagc ggtggtaaat
    15121 gctccaagaa ctgattacat aagtaagtcg tccgaggtag atttgcagct aatgtacgat
    15181 ttatcccggt taagtctgtc ttatagcttg gggaaatgac ataggatctg ctaataattt
    15241 ctgcaacagg tccactcgtc gtcaatccgt acgtttttcc taaaacggat acccagacct
    15301 ttctaaacat ctgaatcatc aattttctac tcattgataa aaaagaatcc ttatcaatgt
    15361 gatgcaccaa atgctgtact tcattcttta tcgaagaaaa attttcattg aaattacctt
    15421 catttatcag ctgagtaatt ttttccaaaa tcgaatcata atcaaacggt ttgattcgat
    15481 ccgtttttat cttttgattt ttctttatca ataaactagc aatcggcaag ctttctactt
    15541 cggattccag catatttaac tgaatcaaga tctcattttt cccaatcgtt aatggttctt
    15601 cttgtgttcg actatacaca tccgcacaac tgattttaat gatattcttc agcttaccaa
    15661 tattcccact aaagggatga gataacatta aagacaaggc ttctcttgaa acaataatat
    15721 ctttttgtaa aacatccgct tcattttgaa aaaataattg aatcagctct aatcgttctc
    15781 gaatggggcg atcttcaaaa gttggcaaca taacactaat ttgaatacgc cgatcaaagg
    15841 tgtctaataa ataattttca cctttttccg tcgtggcgaa aataaaacga accttagcag
    15901 aatgccaatt tacattttca ccgactggac gatagtttcc agtatcaata aatagaaaga
    15961 gcttttcttg attttctttc gataggcgat ggacctcatc gagaaaaaga tagccgttgt
    16021 tggccgttgc caataaacca tcggtatcct tttcagcccc cgtaaaggcc cccttcttgt
    16081 atccaaacag tgttgccgaa agcagttcgg gattgtccgc ataatcggca cagtttaaga
    16141 tagcaaatgg tgcatctttt tcaataattt gctcatggat agcgtattca aaaatttttg
    16201 tggctaaaaa actttttcca acacccgtgg ctccatttat taatacactg agtccgttag
    16261 gcggatattt gacagcggct atacattttt gaatgacctc tctcagactt ccgtcatgac
    16321 caactacaga atgaaaggct tcgtttattt gctccgtatt atctacaacc gcatattcat
    16381 ctaaaggctt ccacaaaaca ggctttcccg gtaatttctc gaccttttta tcttccacta
    16441 atctcgtcaa ataatggctg gcattctgac gagagatacc tagattcttc gaaattttct
    16501 cggcagtgac agaagacata ttccctgtta gtcgtatcag ctcatcaaaa actcgatctt
    16561 tagcgctctt tttcattttt tcctccagtt taatgtgtct aaaagcataa taaactatcg
    16621 gctgcaatat gtctattcta tatttgtatt ccaattttta ctttgaaaca caaatagtcg
    16681 aatatttgcc tcagatatct gacaaaataa tttatctacc aatttagttt ttatctaata
    16741 tgatttttat gttttttcat agctacaaat attgaactgg attcatatat tagttttatt
    16801 aatagattac agaagggtta agtatatttg catagtttcc aattacttta tccttctgta
    16861 actttttcaa tcttagattt actagcccgt ctcgggccct attttacaat ggttttgtcc
    16921 acttttctct ggtagaaaaa cgtggtagaa aaaataaaat aacacaaaaa aagcggcgtt
    16981 tcatccgctt ttggtagaag attggaaaac tcagatagaa aaaggacgat ttttagctct
    17041 ttagaacgtt gataagtccc cattcttact gagttttgcc attatctcct atacatcttg
    17101 aattctaaga ataggtcatt atagatgcct aggtatttcg atcctagatc tttgtagact
    17161 tcaagatggg agatcgtgtc atcagatgga tagaattgtt cgtccgacga aatgtctttt
    17221 ggcaataacg cttttgcttc ttcatttggt gtagaataac caatatactc tgcattttgt
    17281 gccgcatttt ccggttctag cataaagttg atgaaatcat aggcaccttc tttatttttc
    17341 acggtttttg gcataacgat attatcaaac caaagattgg atccttctgg tgggatcaca
    17401 taatgtaaat gttcattttc ccacatcata tcggcagctt cgccagaaaa tgtcactgcc
    17461 acggaggctt cttcattgat catatacatt ttgatctcat ctgccacgat tgctttgaca
    17521 tttggtgtta aatgattcaa tttatttgct gcttctgtca gctgtgccat attcttacta
    17581 ttcaaagaat agcctaaact attcaaagaa agccccataa cttctcgtgc accgtcaatc
    17641 agcatcaaac tgtcttttaa ttccggcttc cacaaatcat cccaatgctt aatttgattt
    17701 gcatcaaaga atttatcgtt atatacgatc cctaaagttc cccaaaaata tggaatcgaa
    17761 tactgattat ctggatcaaa atccaagtct aaaaatcgtg gatcaatatt atttaatcct
    17821 ttgatcttcg aatgatctaa tggaagtact agtttttctt tgatcatttt ttggatcata
    17881 tattcacttg gtatagcgat gtcgtagttt gtcccacctt gctggatttt tgtgaacatc
    17941 gcttcattag aatcaaatgt ttcataattc actttgtatc catattcttt ttcaaatttc
    18001 gtgatcaaac taggatcgat ataatctccc cagttataga tggtaagtac ctttgctcca
    18061 ctcatgccac ttgttttttc aagttgacga acaccaagca gcagcaccgc aataatgatg
    18121 agaataccta aaaacagaga ttgtaatttt ttcatcgcag ccgtgccacc ccctcgcgta
    18181 ctcttctgtt tttcttcagc cgttttggac ggttttcttt actaataaag tagtatccaa
    18241 tgaccaatat catggagaac aagaaaacta acgcacttag cgcattgatt tctaaactga
    18301 ttccttgtct tgcacgagaa tagatctcta ctgagagcgt cgtaaagcca tttcctgtga
    18361 cgaaaaatgt aaccgcaaaa tcatcaagag aataggtaaa tgccataaaa tatccagcta
    18421 tgattcctgg agaaagataa ggcaaaatga tatttttgac tacttggaaa ttattagcac
    18481 caaggtctct agccgcatcg accattgaat cgttcatttc ctgcaatttt ggcaagacca
    18541 tcaagacgac gattggaatg ctgaaagcaa tatgggaaag caatacactc tgaaaaccta
    18601 atccgaatcc tagtagtgta aagaaaatca agaaactagc accgataata acatcaggag
    18661 agactaataa gatgttattc atgctcaaca acgctgtccg tgcacgtcgt tttttcgtgt
    18721 aatagattcc catagcccca aatgtcccga tgatcgtggc gatcaatgca gataagaaag
    18781 ccagcatgaa cgtattcaat acgataatga tcaaacgtgt atcttcaaaa acagcttcat
    18841 aattttccca agtaaatcct gtaaagctgt tcatcgttcc tccttcattg aaggaataaa
    18901 aaatcagata gaagatcggt gcatataaga ataaaaagac gatccctaaa taaagatacg
    18961 accatttaaa ttttttcatt tatgagcgac ctcctttttt cttctcgcct gtcaggagca
    19021 tcacgatgaa catagcaatg attaaaatca caccgattgt agaacccatc ccccagtttt
    19081 gtgtaaccaa gaaatgctgt tcgatagccg ttcctaaagt gatcacccga tttccgccaa
    19141 tcagacgagt cagcatgaat aaagaaagag aaggaataaa tactgcttgg acaccacttt
    19201 tcacaccatt taatgataac ggaaaaatca ctcgtcggaa ggtctcaaag ctattcgcgc
    19261 ctaaatctct actagcactg atcaaagaag gattcatctc ttccaatgca ttgaaaattg
    19321 gcataatcat aaatggaatc tcgatatacg ttgccacgaa cagaaaactg aagtctgtga
    19381 ataatatctg tttcggcccg atcccgataa aatcaaagaa atgattcacg ctgccgtgca
    19441 cactgaaaat cccaataaat gcgtaagctt taagcagcag attcacccat gtaggcaaaa
    19501 taatcagcat cagccataat tgcttgtgct tcagttttgt taaaaaataa gcagtcgggt
    19561 aactgatgat cagcgtgaaa aacgtgatca aaaatgcata ccatacagaa ttgatcgtca
    19621 tagaaagcga tgtccccgac gtaaaataag atagataatt attcagcgtg aattgcccat
    19681 tcatatcaaa aaaagattga taaatgatca ttaatacagg cgcgatcacg aataaaccga
    19741 gccacaaaat ataagggacg gaatagatcc tgcgcattgt tttcattctt gtccccccta
    19801 ttcttcgtag ctgtcaagac gtgcatcgaa ttcttcttca gactcgttga aacgcatgac
    19861 atggatatct tctggctcaa aggccaatcc tactttgctt ccttctttag cttttttcgt
    19921 tgaatggacc atccattcat tttgctgttc atcataacag atgatttcat aatggacacc
    19981 acgaaacagc tgagtatcga ccgttacgac tagttttcct ttatctggtg tcgtgatcgt
    20041 caaatcctct gggcgtaaaa caacttcaac tggttcattt agacgcatcc cgccatcgac
    20101 acattcaaat tgcttaccaa caaactcaac tagattatct tcgatcatta caccgtccac
    20161 gatattgctc tccccaacaa aatcagcaac gaaatgattg attggctcat cataaatatc
    20221 tacgggtgta ccactttgga cgattttccc tttattcatg acaaaaatct catcactcat
    20281 tgccaacgct tcttcttggt catgagtaac aaaaataaac gtgattccta atcgttgttg
    20341 cagatcccgc agttcatact gcatatctgt acgtagtttc aaatcaagcg ccgataacgg
    20401 ttcatctaat agcaatactt tcggttcatt tacaattgct cgtgcaatcg ctacacgctg
    20461 tctttgacca ccagacattt cactaatctc ccgtttttcg tatccaggaa gctggaccat
    20521 acgcaaagca tcttttacct tcttttcgat atctgatttg gacattttct tgatcttcaa
    20581 tccaaaagca acgttatcaa aaacattcat atgaggaaaa agtgcataat cttggaagac
    20641 tgtatttact tgtcgcttat tagctggcaa atcattcaac cgtttccctt caaaatagat
    20701 gtccccttct gtagcttcgg tgaatccagc tataatacgt aggatcgttg ttttaccaca
    20761 gccggaagga ccaagcagtg tataaaattt tccttgttcg atctcaaaac ttactttttt
    20821 taatactgtt tcatcatcat agcgtttcac tacttgatca aatgaaatga ttggtttccc
    20881 cacgttttta ccccctatat tacaaatatg attgcgttgc aacaatcaat acttttgttc
    20941 gttttttgag atgattcctt aattgatgtt gttttgtcgc ttgataatag atcgactgtc
    21001 ctgcttttgc cacatagacc gtctctccta agcttaactc taattcccct tctaatacaa
    21061 aaataaacgt ttcagataat gaaggttcaa acgtcttata ttctcctgca ccttcaaaag
    21121 tcagcaagac aggctccatt tctttttcat tcgaatcagc cacgagccat tccaattcat
    21181 acccgttttc ttcatcgaaa taaaccgtat gatcttcttt tgaataaacc acttgcagat
    21241 tcgctgcttc ttgatgaaaa aattcttctg gagtcacacc taagacttca agaatagaaa
    21301 agaatgtttc cattgatggt gaacttaaat ctcgttccaa ctgggaaata tacccttttg
    21361 acaaatctgt gcgttcgcct agttcttctt gtgtcaaatt cttttggatg cgcaaatttc
    21421 ttaacttctc accaatttcc ataatttcct ccgaatcgaa gttttctaat agtaaacttc
    21481 ctgtttacta ccactcgtat tagtatacga aatttattga aaaaggcaac cctttgtaaa
    21541 attttttttg ctgaatattt ctttatcttt tggaataaat tctagttgtt tgattcttta
    21601 tttttcacct aatttttgtc taaatttacg gaagaacaag aataaaagag taggctttat
    21661 ggtatgataa aagaaaattt taaaaggagt gtctcacgaa tgaacgcttt tcgatctttt
    21721 caggtaataa aggaacctgt tttccataca gcaatcagtg atctgccatt tactgagttg
    21781 gctgaaaatg aagtcttggt aaaaattgcc tattctgatg tcaattataa agatgcattg
    21841 gcaatgagcg agtctggtca ggtgatacga acatatccga tgactccagg gattgattta
    21901 tccggaactg tcgtccaaag taacaatcca cgtttttcaa aagaagatct tgtacttgct
    21961 actggttttg ggcttggcgt cacccatcca ggcggttata gccaatatca aaaagtacca
    22021 ggagactggt tagttcctct accaaaaaat atgacgctgc gtcaagcgat gatccttggt
    22081 acagcagggt ttactgctat gttgtgtgtg aacgctttaa tccgtcaagg tatgacctcc
    22141 gataaaaaag ttgtcgttac tggtgcttct ggcggtgtag gcagtacagc gatagccctg
    22201 ttacataaac taggattcca gtcaatcatt gccttttcca gaaaagaaga gtctgtcaca
    22261 tggctaaaaa gtctaggtgc tttgcaagtc gtacgacctg atgaatttct gccagagaca
    22321 acaaaagctt taggaaaaca gcaaatcgac tatgtcatcg acacagtagg tggcgagcag
    22381 ctaagcagtc ttcttccatt gatctcttat aacggtgcag tagctttatg tggcaatgct
    22441 gggggaatca aactaaacgc gactgtcttg ccttttattc tgcgaaacat ccagttgatt
    22501 ggaattgatt ctgtcaatgt accaatagat caacgtcttt ctttatggca acagatggcg
    22561 gatttgcaga ttgcagatga actggtcgtc caagagatta ctctggatca attgcctgaa
    22621 acagcatcta aactacttgc tggaacacac caaggaagaa cactagtaaa tgtaggtgac
    22681 cataaatgaa gattctaatc acagctggag gaacgactga aaaaatcgat caagtacgtg
    22741 ctattacaaa tcattcgaca ggacgattag ggcaagcatt agctgattat ttagctgcca
    22801 atccggatac cacaatcgat tacgtgacta cgaaacaagc actgaaaccg aaaagacact
    22861 ccaatataac gatttatacg atcgaatctg cactggactt atttttacaa ttagaagaac
    22921 taacgaaaaa agaacactac gatgcgatta tccacagtat ggcagtcagt gattttactc
    22981 ctgcttttag tttttcagaa gaacagctgg caaaaaacct acccacttct tcaacgcaag
    23041 aagaactgga caattggttt gcggaaaatg aacagacaga taccactgtt tcaaagatct
    23101 cttctaacac cgagcatctt gtgctcgttt tgaagaaaac accaaaaatc atttcttatc
    23161 tacgtgaatg gcagccaaaa gcaaaaatca ttggtttcaa actattagta gatgtaccaa
    23221 aggaatctct tctggcagtc gcaaaaaata gtctgataaa taacaaaact gattttattt
    23281 tcgcaaatga tttgacggaa atccatggcg agacacacca cggctatctc ttatctaaag
    23341 atggaacagt ggaagaagct caaagtaaat ctgagattgc tgcattgatt actgagaaaa
    23401 ttagactgga ggaatcaaaa tgaaaaagat cttactaggc gttactggaa gtatttctgc
    23461 ttacaaaagc gctgatctta caagtcaatt cgtcaaatta ggtgcccagg tagatgtgat
    23521 tatgtctaaa agcagcacac attttatcac gccattaacc ttgcaatctc tgtcgaagcg
    23581 cgcagtccat acagacgtca tgcaagaatc aaatccgcag gtaatcaatc atatcgaact
    23641 agcaaaacaa gctgacttgt ttctcatcgc gcctgctaca gcaaacatta tcggcaagct
    23701 agcaaacggc attgcagacg atcttctttc aacagtcgca atggcgcttc cagtgggaac
    23761 accaaaattg attgcccctg ccatgaacac gaatatgtat caaaacccga tcactcagcg
    23821 taatctggaa acattgagat ctgtcggcta tcaggaaatt gaaccaagag aatctctttt
    23881 agcttgtgga gattttggaa aaggtgcctt agctgatatt cagacgatca tcgaacaggt
    23941 catgctgact cttaatgaaa gaaatgctta gaaaaaagga gtacttatga aaaatacaaa
    24001 aaactttaca ttaactgcga tgtttttagc tattttgatt ttattagctg ttacgccttt
    24061 aggttttatc cctatcggtc caatcaatgc aacaaccatg catattcctg taatcattgc
    24121 ttcgatcgtt ttaggaccaa gactaggtgc ttttcttggc ggtacatttg gactgatcag
    24181 catgatccgt agcacagtta tccaaacacc tttgtctttt gtcttttctc cgtttatccc
    24241 agtcatcgga acagaacatg gaagctggaa agccttgctg atcgcgttta ttcctcgcat
    24301 cttgattggg gtggttcctt atttcgttta taaaggtctg cgcaaactaa tgaaacaaaa
    24361 agcagattcc gtttctttat tcttagctgg actttccggt tcattgacca atacgatttt
    24421 agtgatgaat atgatctact ttttattcca gcaagattat gccaaagtca tcggaacaaa
    24481 tttaaatgct gtttattcag ctattctagc ggttattttt acaagtggta tacctgaagg
    24541 aatcgtcgca ggtcttgcga ctgcagcagt atgcaatgtc ttgttaagat acttcaagca
    24601 cccttctctt actaactgat agtcatcgta gccgatctct gatttttttc tcttttggtg
    24661 ttactattga atggaaagat ttgacgtcga aaggatgatc caaatgaaaa tttttgttgt
    24721 aggtgcaaac ggacaaattg gccgacacct gataaaagat ttagctccaa gttcccatga
    24781 aatttttgca ggggtgagag atgttgcttc ccaaacatta gtaaaaaaag agaatgtttc
    24841 gtatgtctct tttgatttaa cttggtccgt ggaaaaaatg accgaagcat tcaaaggcat
    24901 cgatgtattg atttttgctg ccggctctca aggaaagaat cttctgcaag tagacttgga
    24961 tggcgcaatc aaaacagtga tcgcagctga aaatgcgcat gtctctcgct atctgatggt
    25021 cagtgctgtt tatgcagatg aaccagcaaa atggcctgag tccatgacag attattatat
    25081 tactaaacat tatgcagatg aatggctgaa acgaactaac ttagattttg tcattcttca
    25141 accagtcaca ttgactaacg atgaagaagt tacttccatt caattaacta agcctaatga
    25201 gaaggcatca aaaacgatca ctcgctctac agttgctgct gtacttgccg ctctagtgga
    25261 agaaacggat atttctcgta cgactctagt actttctgaa ggtagcaaag agttgaatac
    25321 agcattccaa gaatgggcaa aggaggaata gatccatgcg tgccatttca atcaagcatc
    25381 ctggttctgt agatgtatta gaacaaattg aagtgcctgt tcctaaacca aaacaaggcg
    25441 aattactgat caaagtccat acagcagctg tcaatcgaac agatattatg aaaagagaat
    25501 cagtctcttt gaaacctccc taccctattt tgggagtcga agtagctgga gaggtagttg
    25561 agaataaaag tgatatctcc acttttactc ctgggacaaa agtatacgga ttagtcaatc
    25621 taggtggtta tgccgaatat gctgtgatgc ctgccgatcg tgcaatcctt ctccctgata
    25681 cgctagacta tgtttctgct gctggaatct cagaagtttt tttgactgct tatcaaacac
    25741 tttactggct tggaaaatta caagaaggcg aaactgtctt gatccatgca ggagcaagtg
    25801 gtgtgggaac agcagcgatc caattagcta gacagctttc tcacgcgaaa atcatcgtta
    25861 ctgcaggatc agaagaaaaa ctcgcctttt gtaaagaact tggtgcagat gaattgatca
    25921 actataaaaa acaagacttt caagaagagg tacgaaaaat cactgaaggt cggggagtcg
    25981 atgttatttt ggatttcatc ggcgcttctt actgggaaaa gaatttagct tctatagccg
    26041 ttgatggacg ttgggtcttg attggtatgc taggtgggac catcgtgcct gagatagacc
    26101 ttggtgtatt gattgggaaa cgtgtccaac tgcttggtac attattgaca cctagaagtg
    26161 atcagtataa agcagagctc gcacaaactt ttatgcaaaa agctgggcct tttctagaaa
    26221 atggcaagat tcggccaatc attgaccgta cattccaatt cagcgcagtc aaacaagcgc
    26281 atgaatatat ggaagcaaac aaaaacatcg gaaaaatcat tttgaagata actgaataaa
    26341 atcatgctga t
//
LOCUS       DACEPD010000033        26161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.033, whole genome shotgun sequence.
ACCESSION   DACEPD010000033 DACEPD010000000
VERSION     DACEPD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.033"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(64..1095)
                     /gene="queA"
                     /locus_tag="IW797_001305"
     CDS             complement(64..1095)
                     /gene="queA"
                     /locus_tag="IW797_001305"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ1224089.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKIGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1288..2463
                     /locus_tag="IW797_001306"
     CDS             1288..2463
                     /locus_tag="IW797_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1224090.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESDTPEESKEV"
     gene            2467..3108
                     /locus_tag="IW797_001307"
     CDS             2467..3108
                     /locus_tag="IW797_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1224091.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3105..4022
                     /locus_tag="IW797_001308"
     CDS             3105..4022
                     /locus_tag="IW797_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1224092.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVNNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4019..4690
                     /locus_tag="IW797_001309"
     CDS             4019..4690
                     /locus_tag="IW797_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1224093.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5232..5720)
                     /locus_tag="IW797_001310"
     CDS             complement(5232..5720)
                     /locus_tag="IW797_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ1224094.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVSLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRTAKSSCDGC
                     SP"
     gene            complement(5921..6187)
                     /locus_tag="IW797_001311"
     CDS             complement(5921..6187)
                     /locus_tag="IW797_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ1224095.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6187..6450)
                     /locus_tag="IW797_001312"
     CDS             complement(6187..6450)
                     /locus_tag="IW797_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ1224096.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDNIDWEAL"
     gene            6925..7110
                     /locus_tag="IW797_001313"
     CDS             6925..7110
                     /locus_tag="IW797_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1224097.1"
                     /translation="MALSYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7252..9075)
                     /locus_tag="IW797_001314"
     CDS             complement(7252..9075)
                     /locus_tag="IW797_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ1224098.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9671..12376)
                     /locus_tag="IW797_001315"
     CDS             complement(9671..12376)
                     /locus_tag="IW797_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ1224099.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSENLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIEAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGVLSKADQIDFESLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPTLKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12682..13629)
                     /locus_tag="IW797_001316"
     CDS             complement(12682..13629)
                     /locus_tag="IW797_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ1224100.1"
                     /translation="MRRFAIKGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13819..14088
                     /gene="rpsN"
                     /locus_tag="IW797_001317"
     CDS             13819..14088
                     /gene="rpsN"
                     /locus_tag="IW797_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1224101.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14264..14626)
                     /locus_tag="IW797_001318"
     CDS             complement(14264..14626)
                     /locus_tag="IW797_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1224102.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14637..15758)
                     /locus_tag="IW797_001319"
     CDS             complement(14637..15758)
                     /locus_tag="IW797_001319"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ1224103.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQIKKLPAGEGIGYGETYITSE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15774..16124)
                     /locus_tag="IW797_001320"
     CDS             complement(15774..16124)
                     /locus_tag="IW797_001320"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ1224104.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16254..17765)
                     /locus_tag="IW797_001321"
     CDS             complement(16254..17765)
                     /locus_tag="IW797_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ1224105.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18087..19439)
                     /locus_tag="IW797_001322"
     CDS             complement(18087..19439)
                     /locus_tag="IW797_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ1224106.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAVKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19592..20668)
                     /locus_tag="IW797_001323"
     CDS             complement(19592..20668)
                     /locus_tag="IW797_001323"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ1224107.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLIFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20864..22609
                     /locus_tag="IW797_001324"
     CDS             20864..22609
                     /locus_tag="IW797_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ1224108.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRIPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAKNGTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTRASQKTETVHEKEFGSFTFRASTSTEDPVAPYTVNYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGVFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWSSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22858..23325)
                     /locus_tag="IW797_001325"
     CDS             complement(22858..23325)
                     /locus_tag="IW797_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ1224109.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVIIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGLRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAIDQGLPSTEDSR"
     gene            complement(23309..24682)
                     /locus_tag="IW797_001326"
     CDS             complement(23309..24682)
                     /locus_tag="IW797_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224110.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLSWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFILAKEMGEPIHLLLITLNRESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25006..25494)
                     /locus_tag="IW797_001327"
     CDS             complement(25006..25494)
                     /locus_tag="IW797_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1224111.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25516..26118)
                     /locus_tag="IW797_001328"
     CDS             complement(25516..26118)
                     /locus_tag="IW797_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1224112.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLLFFFVLILYAYHHGIFRSSTSLQV
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7499 a         5559 c         4834 g         8269 t
ORIGIN      
        1 ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg gcttttcata
       61 accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct cttcaatcgc
      121 atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta acatgactaa
      181 agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga attcatagcc
      241 aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat taaatttcgt
      301 tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt tcccgccatt
      361 tttacgtact tggttcaatc gctcagctgc ttcttccgtc aaacggtaga attcactatg
      421 catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta accctacatg
      481 tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta attctttcgt
      541 aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat agacagtttg
      601 atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca tttctcccaa
      661 tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc gtccgccgtg
      721 ttccaattct tctttcacta ccgcttgcaa tctgccatca ccaaaagaga ttcttgtccc
      781 aactttggca cgtttcgcag gttttatcaa tgtctcccac gtatctcctt gtgtattcgt
      841 tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata aacgagcagg
      901 cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt caatgatttc
      961 atggaaatgt ttatcttcca tctctccaat tttccgatcc aaaacaagta aacgagaatg
     1021 atcgcggtct tttaatggtg tttgtgcgat caactcttcc ggcaagtcaa aatcaaaatc
     1081 ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt ctatcatgtt
     1141 tttcattctg ttggcaaaat cagcactttt tcacttctat tttttgtttt cctaaataaa
     1201 aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag atagttcttc
     1261 attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc aaaaatttat
     1321 aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg tgagttcatt
     1381 tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat caaccgaatg
     1441 actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga aattgatcca
     1501 gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat gcctcacatg
     1561 acgatccgtg aaaatatcac ccttgtccaa aaattgttga aagtcagtca agaagaacgg
     1621 aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat gttagatcgc
     1681 tacccacatg aattatctgg tgggcagcag cagcgtattg gagtcgttcg tgcccttgca
     1741 gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc catcactcgt
     1801 gattctttgc aagaccttgt caaagattta caagagcgct tgggaaaaac gatcgtattt
     1861 gtaacccatg acatggatga agcgattaag ctagcaagcc gtatcgccat catgagtgaa
     1921 ggccgtgtga tccaatttga tacaccagaa aatatcttac gtcatcctgc aaacgatttc
     1981 gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc gacaagagtc
     2041 ggagaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt acaagaagca
     2101 atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa ttcgaatgta
     2161 ttaaaaggat ttatcgatgt agaaacgttg aataaaaagc gaggaaaagt atccagtgtc
     2221 ggcgacatat taaataaaca tgttttttac gtaaaagaat ccgcctatct tcgtgatacc
     2281 ttgcaacgca ttttaaaacg cggactgaaa tacgtgccgg tcgttgatga acagaacaaa
     2341 gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt gatttgggga
     2401 gaagatcccg caactactga tgttgcagag tcagatactc ctgaagaatc taaggaggtc
     2461 tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa aggttttgaa
     2521 catctttata tttcgggact tgctttgatc attggtatca tcattgctgt accgcttggt
     2581 gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac cagtgccttg
     2641 cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg cgtaggtaaa
     2701 ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg taatacgtac
     2761 attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat ggggatgact
     2821 gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat catggctggt
     2881 attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta tatcggcgct
     2941 ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga tctcattttg
     3001 gcaggtacga tccctgtaac gattttagca ctattggctg attggcttct aggtctcttg
     3061 gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa cgtatactaa
     3121 aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttctttc cctggtcttg
     3181 ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca gaagcacaga
     3241 tcttagcaag cctagttgcc ggaatggttg aacattatac agataaaaat accgcaatca
     3301 tcaacaatct ggctaccaca actatcaacc atcaagcgat gatgaacgga gatgcttcaa
     3361 tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt cctcctgaaa
     3421 aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga tacgatcaaa
     3481 cgtggttccc gtcctatggt tttgaaaata cgtatgtctt tcttgttcga aaagacaccg
     3541 cacaaaaata ccatttatct aaagtcagcg atctgaaaaa tgtagcggat gaattagtag
     3601 ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc ttccaagaaa
     3661 cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt tatgacgcag
     3721 tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg atcgccagtt
     3781 atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat gctgctccag
     3841 ttgtaaacaa caaaatatta gctgatacgc ctcatttaaa agaagcctta aataagttag
     3901 gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat aataatctag
     3961 tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt gaagggaagt
     4021 gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc aggaaaatgg
     4081 ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg gtgtcctctt
     4141 tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta aattagcaaa
     4201 ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca tgatctccat
     4261 tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat ttttgtattc
     4321 cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca aaaacatcct
     4381 tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg tagagttacc
     4441 actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg ccatcgggat
     4501 aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta tccgaggaac
     4561 caatgcaaca gatggcacat cgattatttt agcaggtgca ttgccaactg cattaatggc
     4621 gattatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag ccagccgtac
     4681 atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg gtcgcaaagg
     4741 cttttttagt tctgtcattt tttaaggcaa taatccactc tgattcttcc ctccttatgg
     4801 gcaaagagtt gcttttctta gctatttatc cattccgtat actccaactt catactgcca
     4861 acgggttcat ctctgcatcc accgactaag tttttttctt atccgctaag gcattttgcc
     4921 aatccatttt cccatcactc gtcatggaat tcataccaca tgatctctaa atactcgtct
     4981 gcttgttctt tgaattgggc atcaattctt acatcctttg gcgtggcttt catttaagcc
     5041 ttttccatca gtcctcgatt gttcgatgcc ttcagctaca tcatcaaaac tggtgactgc
     5101 cgtatcggta acttcgatca tcttttatac aatcgggaac ttgccatcct catccttggc
     5161 taaaaacata tctagccatt ccagcttctc gccatgggag gtttgcggtt cctaaaaccg
     5221 gtacacgatt cttatggact gcatccgtcg caactagact tggcggtacg atgatccttc
     5281 accagaagag cacccccgta aaccaattga tcgatatatt gccaataaga aaagacattg
     5341 gcgtaaaaga tatcgatccc tcgtgatgca ttgccgttga tactgctatt catggccgaa
     5401 agcgccacga ctttgacttc ttccgtttga gtgacattca tgttcggcaa tgtcgctttg
     5461 gatacccgct ctgctaaaga aacgacactg gtattgtatt tcgtatctgt atcttttcca
     5521 aaagaccatg ccaacgaatc ctctgggaac caataagacg actccggttg attgtccatt
     5581 gcttttttca acatactttt ggctgtttct cgataaacga catttccaat tgtcttcgcc
     5641 tcactttggc gataatcgat ggtcgttgcc actgccaaca ctaacaacga tgctccaatt
     5701 ttactgattt tccttgtcac acttcttcct ccttgtttta cataaacccg tcaacattcc
     5761 attcctaaac tcaaacaatg acccttcaaa ggtggagata taggaacaaa ttaaagaaat
     5821 catgcaataa atccaagaag gttggtttta gaacaaaaaa agagtctcct ttttggggag
     5881 actctttgtt aactatctaa aaaggttggc tcttctcact tcactcataa tgtgtccagg
     5941 cagaataaat ttccacaact ttattttcct catcaacttt gtaaaccact cggtgctgtc
     6001 tattcagcct gcgtgagtat ctgccttcat gtgtaggtct cagcttttca aaagactgcg
     6061 tgggcatata tggatcttcc ttcaatgtct gaatcacttc ttcaaattgt ttctttaaat
     6121 tcgattgctt gatttttctt aaatccactt tcgcagagtt tttgattgcg actgtatagt
     6181 tactcatcaa agagcttccc aatcaatatt atcgatattc gtaaagccac tgtcgtcttt
     6241 ttcgcggtct cgtactttat ctaaagtacc tgtttgctca agaaaaaggg tttcttgaat
     6301 cgctctccag tcttctagcc caatcaatac agcattgttt ccactacgat cactaataat
     6361 ctcgatttca gtatgatttt catttacctc tttcaataac tgataaaaat ttttacgagc
     6421 tgtgctagga ttcatcgttg tattagccat tgtctctcct cctttttctg atataattat
     6481 aacgtacttg attaagtacg tcaaaaaata caataaagca cttaaattca gcaaactttt
     6541 cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc cacgctcact
     6601 tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca acgtatcatc
     6661 tttagcaaag gtcaaatcca aagcctgtga gtctatgcaa caacaccaat acacgaaggt
     6721 tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg cacgaaagat
     6781 ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct caaaaatgat
     6841 tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc ttagaccgtt
     6901 gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa atggtcactt
     6961 gacgaggctg agatttctac ttatgaagac acatggcact taagagtcga ggtaaaggaa
     7021 gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa ttatgatcaa
     7081 gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt tcatacgctg
     7141 ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca aaacagaccc
     7201 tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg tttatttctt
     7261 caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc gcaatcttaa
     7321 ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta caaattgagg
     7381 aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga tatctacata
     7441 acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa aacggacatc
     7501 tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat cttcgtctag
     7561 cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca acgcaccgat
     7621 atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat aatcatgtag
     7681 ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat catggatctt
     7741 ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt cccctaaccg
     7801 atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacctgtaa tattggcaat
     7861 cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc ctgtttgaga
     7921 taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt ctgttgagcc
     7981 atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc cattggaata
     8041 gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt ttgccagatt
     8101 gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta aaatcagcgc
     8161 tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt taccaatttg
     8221 agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga taaatgtgat
     8281 ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag cggcattttt
     8341 tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc cagttaaaga
     8401 ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg gcacagccgc
     8461 tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat ataaaccaac
     8521 aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata ggaacgatgc
     8581 agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg tcacagaaat
     8641 agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag cacctgctga
     8701 gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg aaatcaagcc
     8761 agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat gaggataagc
     8821 atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa caaaagcggc
     8881 aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga caacgatttg
     8941 ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca aagcagcaaa
     9001 tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta ctaacaacct
     9061 ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag ttttatagtc
     9121 gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag tcaataaact
     9181 ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa cgctttaata
     9241 cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat aaaaggatag
     9301 aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg aaagagagat
     9361 gtaaggttgc tttctaccca tcattcttac tactcgaagc atagcgcagt tgtcacaacc
     9421 tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc aaattaaaaa
     9481 cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag attttttaca
     9541 acctcttggt acacggtgtt caatcagctg accctcggca ttagctcaaa aaacggcaaa
     9601 atatgaggaa ctatttttcc attttttctc taatactcag gtcaaaacgc tgatttcaca
     9661 acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt
     9721 tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga
     9781 tcatgatcca gattgctttc gtagcatttg gccagtaaat gcctcctgca gattcccgca
     9841 acaagtttac cgcatgcgta aatggcaaga acggattgat catttggaag aatttccctg
     9901 agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag
     9961 cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg
    10021 ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta
    10081 gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg
    10141 tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta
    10201 aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg
    10261 cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt
    10321 gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcatctagt ttcaataatt
    10381 tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt
    10441 ggatgccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag
    10501 ctgtttcgac ctctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt
    10561 ttagtgttgg ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaagga
    10621 tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca
    10681 tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt
    10741 tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagcttagca
    10801 gagattcaaa atcgatctgg tccgctttgc ttagcacccc ttgtgcagtc ccaatcgtat
    10861 cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag
    10921 caatgatgcc attgatatta ttaacaacgg cctcaatttg atctagcaat tcatcaatag
    10981 ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc
    11041 ttagcaaggg ttttaattga tttagctgat caataattgg ctgcaatttc gtatttccag
    11101 cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag
    11161 atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca
    11221 gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca
    11281 cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt
    11341 tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat
    11401 ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt
    11461 catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta
    11521 gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg
    11581 cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat
    11641 ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag
    11701 gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt
    11761 catctgttga taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt
    11821 cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat
    11881 ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat
    11941 tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac
    12001 tcagcagatt ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac
    12061 ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt
    12121 tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg
    12181 gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata
    12241 atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa
    12301 gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat
    12361 ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg
    12421 tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg
    12481 gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac
    12541 tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta
    12601 tacgcaaaaa agctaaagca ttttttgtct ttgcccctac aaaaatgttt cagctttttt
    12661 cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg
    12721 atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat
    12781 tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc
    12841 tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg
    12901 caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg
    12961 gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc
    13021 agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt
    13081 ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg
    13141 gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc
    13201 tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac
    13261 cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg
    13321 caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac
    13381 atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg
    13441 tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc
    13501 ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct
    13561 gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaacctt taattgcaaa
    13621 tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct ttattttgac
    13681 aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaataattgt cttttttcgc
    13741 gaaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat
    13801 aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa
    13861 gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa
    13921 ggcttgcgca aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc
    13981 gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa
    14041 ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc
    14101 tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc
    14161 gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg
    14221 gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag
    14281 tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc
    14341 gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca
    14401 acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg
    14461 gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat
    14521 ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca
    14581 cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat
    14641 tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta
    14701 cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc
    14761 gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat
    14821 acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt
    14881 cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc ttcagaagtg
    14941 atatatgttt ctccatagcc gattccttca cctgctggga gttttttgat ttgaatcaat
    15001 tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag
    15061 ggattcagtc cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc
    15121 cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttt
    15181 agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct
    15241 gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca
    15301 gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct
    15361 tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgcc
    15421 tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt
    15481 gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac
    15541 tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc
    15601 tcagctgttt caatagctcc atgaccgtat ccgtttgctt ttactactgc aaacaattct
    15661 tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt
    15721 atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct
    15781 tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg
    15841 ccttcatgag gtgaacgcgt cacaacaggc tgaccgtttt cattttttaa aatctcgaca
    15901 tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa
    15961 gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc
    16021 atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg
    16081 atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc
    16141 aataaattta gaacactctc attctaccaa attataacca ttttgccaca aataagtcca
    16201 caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg
    16261 ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt
    16321 tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg
    16381 tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc
    16441 tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt
    16501 gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta
    16561 tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca
    16621 actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc
    16681 atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg
    16741 aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag
    16801 ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat
    16861 ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc
    16921 aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg
    16981 cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt
    17041 gttagttcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct
    17101 tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc
    17161 acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt
    17221 gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct
    17281 tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt
    17341 ccaagtttca atgtgtggcg gttgatgtgg tcaagcatac gtcctggtgt acccacaacg
    17401 atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg
    17461 gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata
    17521 gctaactcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt
    17581 ttttctaaca tcggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca
    17641 ataacgtctt ttccagctaa tgctagtgga atcgtttctg attggatagg tgttgcttct
    17701 tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat
    17761 ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc
    17821 gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca
    17881 ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc
    17941 caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct
    18001 atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt
    18061 ttcgttttct gcaagtttac agatttttat ttcatttgct gaagctgttc taccacttca
    18121 aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc aagattttgt
    18181 tttacagcat caatcaattt attcttttct ttttcaaaat agaacacagg tagttggggg
    18241 tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg
    18301 gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc
    18361 cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg tagattttta
    18421 aaagtatcta ataccagtcc catggctgtt ggatttgcat tatagacatc gctcaaaata
    18481 gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg
    18541 agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc
    18601 aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact
    18661 acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca
    18721 ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca
    18781 tctgcgggca caacgagtaa tccgtctggt acaagtccgt cagtgatttc cattttggct
    18841 ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt
    18901 gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg
    18961 cccatttcca agacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga
    19021 ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca
    19081 gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct
    19141 gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct
    19201 acaggaataa ctggaaaatc ttttggagct tgttcaacag gcaagctcca aaatgaagca
    19261 gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga
    19321 ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt
    19381 gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt
    19441 gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg
    19501 actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac
    19561 gcaatcactc cagcttcttc tttatttctc tttattcatt tttttcaaaa aaagattgac
    19621 gctgatggta tcgattcatt cctaattgaa tcagttcttc aatcaaatca ccgtatttca
    19681 agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagaat
    19741 ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcacac cggctcaatc
    19801 cgcttccgcc taacatcgtg taagctaact tcgcgtactc ttgtgctttt tgataaactt
    19861 cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat
    19921 aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat
    19981 ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag
    20041 aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg
    20101 taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac
    20161 cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta agtactggta
    20221 cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca
    20281 tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga
    20341 agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc
    20401 cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc tccgtctgtc
    20461 cacttgggtc ccatgaaaga tgcaagacat ctttgcttgc aggtttttct gttagtaatg
    20521 gacctttgac ccattgtcct tcttttgtaa taaatatgag ttgaacttgg taataattat
    20581 aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc
    20641 gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag
    20701 tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa
    20761 acgagttggt ttctaaaaga acaaacgtca tttatttttt atcagctaga ttatttgtcc
    20821 cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc
    20881 acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag taccaaactt
    20941 tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt
    21001 catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca
    21061 aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac
    21121 gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt ccaaatcgcc
    21181 ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgactcggt
    21241 cgtcgttaag atccaacacc ctaacatcgc cgaagagatt cgacttgatc ttcaactatt
    21301 tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg
    21361 ggtactccaa gaagtgaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca
    21421 aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca tccccaaaat
    21481 ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt
    21541 gaatcatttg atgaatgcca aaaacggaac agaaactttt atcaccggta ttcaaaacaa
    21601 gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac aggtatttga
    21661 tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa
    21721 agaagaacag acacgagcta gccaaaaaac tgaaacagtc catgaaaaag agttcggttc
    21781 ctttactttt cgagcctcta cttctacaga agaccctgtt gcgccatata ccgtcaacta
    21841 catcgatttt ggtatgatgg ggcatctttc tgcagggctt aggcaaaaac tcacacaagc
    21901 cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtct tgcgactttg
    21961 tcagcaagaa ggcgtgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga
    22021 acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca cacacgtcgt
    22081 gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc
    22141 attcgggaca ttagaagggg tgatccgtgt ccttgaccct gaagtttccc tcatggaagt
    22201 cgcttcgcct tttgcccaac attattttct cactcatctg gatgtagaag atacattgaa
    22261 acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct
    22321 ccaccatcta ttagaaatgt ggtcttcggg acaaggaaaa gtcaatttgg aattgaaaaa
    22381 gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat
    22441 tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc
    22501 agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt
    22561 tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt
    22621 gacagaaaga atcagcttca agaaataaga aggggtttta cataaattat ttctcaaatt
    22681 tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa
    22741 tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaaggaaca aggaatccat
    22801 ttatcctgtt tccctgttcc ttttttggtg tattcgagca taaataaaat accttcgtta
    22861 cctactatct tctgtacttg gtaaaccttg gtcaattgcc ttttttaacg cttgacgaaa
    22921 agacatatga tctttctccg taaagggttt tggacccttt gtccttatgc cagcttttct
    22981 taactttcca ctatgataac gttgttctag aagcgtgccg atattcgctt cgtcaaattc
    23041 ttttccagag tgatggaaca ctcggaggcc cttcggcaat acaagtgaag ctaaaccata
    23101 atcttgcgtg atcaaaaagt cattcttctt aaccataccg acgattttat aatccgcact
    23161 atcagctccg cggtctacgt agacgatttg gacatgatta gcgtattctt tggtcgtata
    23221 atggtccaca ctggttacga taatcacgtc gagaccatag gattctccta attttatgac
    23281 atcttctttt actggagaac catctccatc aatgaatagc ctcattcttt ttcgccttct
    23341 tccataaatg ataaacagtt actaatccgc tcagtaaaag accaacattg atccattttg
    23401 ccaagataag cagttgtaca ctttctatcg gcactgattc acggttcagc gtgatcaata
    23461 atagatggat tggctctccc atttcttttg ctaaaataaa caaaagcgtc atcaataaaa
    23521 actggtggat cacactccct ttattttgag tagtatactt gacaacgatc cggcgatcca
    23581 ttggaatagc tgcatcagtt tgataatatt ccttatttag ccagctgcct gttactacgt
    23641 gccagaaact aagcagaaag acacctatcg caaaaccatt tggaatcaag aggaccaata
    23701 aagaagaact cagtccaagc aattgcatag ccaatggaga ccatgataaa cgtttcaaaa
    23761 tccatggtcc gactattttt gctgcaatca agcctgctgc ataaggcaaa tacataaaaa
    23821 tcgaaagttt ttctaatccg tacagtatgc caacataaaa actgctaaac aaaaaaagga
    23881 aattccctag cattccattt agaaaagtaa acaaatttag ccagcttggc aacttccatt
    23941 ccttcttctg gtgtcctaaa acaaacacga acaagatgaa cagcagcaag aaaccataaa
    24001 tagcaagatc aaaaagcctt tcatcgaata acaatcgggc atttctcaaa agaaacaata
    24061 agccaaagaa tatcaaaaat aaaacaagct ctcgcgtagc taccagattt cttttgatgt
    24121 ctttgaaatc cagttcgtaa cgcggataat gactaaccgt atggtaggcc ataacgtaaa
    24181 aaagtgtata aatagtaaag cttgctgcaa tctgagtcgg tgcaggcaac tcaatcgctt
    24241 gatacaggag aaatagccag atagctgcaa ataagtactt tttcccatcc atattgataa
    24301 aaccttgcgt cttttcgtga aaattgaccg tggtattagc aggtacaagc catgctgcac
    24361 tcagccctag caaaacactt gaaaaataaa ataaaggaaa ataaaatacg cctaatattc
    24421 caagcaatga acccgcacca ccaatcagca gagcgatcat cagcaacgtg taactatcta
    24481 gaccaaatcg tagactgttg actaaaaata gtccagtcat ccgaaaggca taaaacagcg
    24541 taaatggcaa aatcgtcagc caattatgcg agtctgttag tcccaaaaat aaaagataag
    24601 gaataaaaat aaccgtgtta cttaagataa agggcgttgt ttgtgtaaaa ttacgcccaa
    24661 atcgtttgag tttttcgaac atgacaatgg ctcccttaaa cagtcttata actaatctgc
    24721 tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa
    24781 aaaaaataca gaaagaagca taagcttctt tctgtatttt tacggtactt gattactcaa
    24841 gcagacaagc ccttctccac aaaagtcttg tccgacaccc ctgcatccgg caggtgataa
    24901 agatagaaat caattatcct gtgcctccaa aatcaattag gaactctgta aaccagagtc
    24961 cttctcgacc cgtcgcataa atttacttta acacagttcc tatgtttatg cgagagcttt
    25021 tttcatttta taatcaacca tctggaatcc ttgaccgata tctgaaataa gttcttctgt
    25081 aatctcaaac ccttcatgct tataaacctc gattgcttga tgatttccct ggttcactcg
    25141 caaaaagatc tctctttcct ttgtttgcgc agcaagttct tcataccagc gaaaaaccga
    25201 agaagtcaat ccttggccac gtactttatt tagcaaatat aatttactta aataaatttt
    25261 tccatttatc tcttcataag cagtgtaacc gaccgtcttt tcttcgcttt ttaataaaaa
    25321 gtaggacaca ccttctgtca gttctttttg aatctgtttt tttgactgat aagttttcag
    25381 catgtaatcc acttgttttt gaccaatgat cggcacaaaa gcttccggcc atacttcttg
    25441 tatgactgcc atcagctcat ccagttcatt ttctgttact tttatttttt ccatacaccc
    25501 acgtcctttt ttacattata aatgaccttg cgcttgatgg aataaaaaga gcatgatcat
    25561 gctatacaca cctaccgacc atggtttcaa gaaaatcaca atctgcataa attccctaaa
    25621 agataatttc gtcaaaccag ctactagaca gatcaagtcg tctggcgcaa aggggagcag
    25681 caaggtaacg actagtactt ttttgatatc tttcgttttt cttgtcagca attcatcaaa
    25741 tttctgatac ttttttggcg ataaaataag ctggacaaag gctacgccat agtaacggac
    25801 aagtaaaaat ccgattgctt ccccaatcac taggccaata caactatata aaagcccaaa
    25861 tccattgcca aacatcagca tgcccgctac tgaggagatc cctcccggca aaataggaat
    25921 gatcacttgg atcacttgca gcagaacaaa tatcatcact gcatacttgc caaattgctg
    25981 gataaacact tgcaaagacg tgctcgatcg aaaaatccca tgatgataag cataaagaat
    26041 caacacaaag aaaaacaaca gaccaataat gggcatgatc tgaagacatt tttgtaaaac
    26101 tgatcctttt ttagacatct atattcccca catctttttt catttattaa aactgcctcc
    26161 t
//
LOCUS       DACEPD010000034        26028 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.034, whole genome shotgun sequence.
ACCESSION   DACEPD010000034 DACEPD010000000
VERSION     DACEPD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.034"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(44..682)
                     /gene="plsY"
                     /locus_tag="IW797_001329"
     CDS             complement(44..682)
                     /gene="plsY"
                     /locus_tag="IW797_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ1224113.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            809..1243
                     /locus_tag="IW797_001330"
     CDS             809..1243
                     /locus_tag="IW797_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ1224114.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1409..3454
                     /gene="parE"
                     /locus_tag="IW797_001331"
     CDS             1409..3454
                     /gene="parE"
                     /locus_tag="IW797_001331"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ1224115.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3466..5916
                     /gene="parC"
                     /locus_tag="IW797_001332"
     CDS             3466..5916
                     /gene="parC"
                     /locus_tag="IW797_001332"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ1224116.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6193..8424
                     /gene="pflB"
                     /locus_tag="IW797_001333"
     CDS             6193..8424
                     /gene="pflB"
                     /locus_tag="IW797_001333"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ1224117.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8668..9429
                     /gene="pflA"
                     /locus_tag="IW797_001334"
     CDS             8668..9429
                     /gene="pflA"
                     /locus_tag="IW797_001334"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ1224118.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9514..10440
                     /locus_tag="IW797_001335"
     CDS             9514..10440
                     /locus_tag="IW797_001335"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ1224119.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10622..11515
                     /locus_tag="IW797_001336"
     CDS             10622..11515
                     /locus_tag="IW797_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ1224120.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11649..11774
                     /locus_tag="IW797_001337"
     CDS             11649..11774
                     /locus_tag="IW797_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1224121.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11908..12540)
                     /locus_tag="IW797_001338"
     CDS             complement(11908..12540)
                     /locus_tag="IW797_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224122.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12688..13230)
                     /locus_tag="IW797_001339"
     CDS             complement(12688..13230)
                     /locus_tag="IW797_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ1224123.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13365..13754
                     /locus_tag="IW797_001340"
     CDS             13365..13754
                     /locus_tag="IW797_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224124.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13758..14522
                     /locus_tag="IW797_001341"
     CDS             13758..14522
                     /locus_tag="IW797_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1224125.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14651..15598
                     /locus_tag="IW797_001342"
     CDS             14651..15598
                     /locus_tag="IW797_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1224126.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15728..16468
                     /locus_tag="IW797_001343"
     CDS             15728..16468
                     /locus_tag="IW797_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224127.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16759..17427)
                     /locus_tag="IW797_001344"
     CDS             complement(16759..17427)
                     /locus_tag="IW797_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1224128.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            17561..17848
                     /locus_tag="IW797_001345"
     CDS             17561..17848
                     /locus_tag="IW797_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224129.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            18264..19802
                     /locus_tag="IW797_001346"
     CDS             18264..19802
                     /locus_tag="IW797_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ1224130.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            19799..20764
                     /locus_tag="IW797_001347"
     CDS             19799..20764
                     /locus_tag="IW797_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224131.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            20751..22073
                     /locus_tag="IW797_001348"
     CDS             20751..22073
                     /locus_tag="IW797_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1224132.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      22136..22240
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22314..23057
                     /locus_tag="IW797_001349"
     CDS             22314..23057
                     /locus_tag="IW797_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1224133.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            23072..24064
                     /locus_tag="IW797_001350"
     CDS             23072..24064
                     /locus_tag="IW797_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1224134.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            24076..24813
                     /locus_tag="IW797_001351"
     CDS             24076..24813
                     /locus_tag="IW797_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224135.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            24803..25525
                     /locus_tag="IW797_001352"
     CDS             24803..25525
                     /locus_tag="IW797_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1224136.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(25609..>26028)
                     /locus_tag="IW797_001353"
     CDS             complement(25609..>26028)
                     /locus_tag="IW797_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ1224137.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         8954 a         4426 c         5429 g         7219 t
ORIGIN      
        1 gatttgtcac actcccgttt tatctctttg gaatgaaaaa gtatcaggat tttttcttgc
       61 tgctattcaa accaaacggg attcgacttt cagtatggtt tctgatacgc ttgatattgt
      121 ctttatgccg ataaaagata aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat
      181 tgggcttagc caaaatcaca ggagctacat aaggaaggac gatcgtagat aaagtgatca
      241 atactgcact gatcatactg ctcaaactca ccatacttgt acaaaataaa ctaacgataa
      301 agatgataca agaataaaca aaaaatacag gattaaatcc taaaagcatt cctgcacttg
      361 tcgcaacagc ttttcctcct ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg
      421 cgcatacccc aaaccaaaga gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag
      481 tagccaatgt accttttaaa atatccataa gcaggacaac aatcccggct gttttgccta
      541 atacacgaaa tgtattggtc gttccagtat taccgctccc gtgttgtcgg atatcttttt
      601 tgaaaaacaa tttgccaatc cagacaccgg aaggaataga acctaaaaga tatgctaaaa
      661 taagtaataa gatcaatttc attttttacc ctctttcaag ttgaaaacta tcctttattt
      721 tagcatgaat aggtaacagg aacaattgaa aaaatgaaag atacaagtat aatagatgga
      781 ataaaaagaa aagcaagggg tggcagaaat gtctgtaaaa aatccaacac aagaaacgat
      841 tttcaactat ttggaagaag caaaaaatat tgccgtcgta ggcttgagca ataaaccaga
      901 acgaacaagt tacatggttg cacaggtatt gcaagacaat ggatataaaa tcataccagt
      961 aaatccatta ttggctggtg aagaaattct aggcgagaaa gtctatgcga atttgacaga
     1021 aatcgatgat ccaatcgata tcgtagatgt atttagacgg agtgaatttt tacctgaaat
     1081 agcaggtgat tttttgaaaa ctgatgctaa agtgttctgg gcacaattag ggattgaaaa
     1141 caaagaagca gctgagatgc taagaaaagc aggacgtaca gatatcgtaa tggatcgttg
     1201 tattaagatt gaacttggta agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag
     1261 agatgatcga tagaggggaa tgtcgaaaaa gcggttagga gataattaaa attgatttct
     1321 cgtctaccct cttttttttc acgacctttt ctagtatgat aatcgtggtg cggttttgcc
     1381 gcaaagtatt attgaaggag cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga
     1441 ctcgtccatc caagtattgg aaggattaga ggcagttaga aaaagaccag ggatgtacat
     1501 tggatccact gacagccgag gcttgcatca tttagtctat gaaatcgtcg ataatgcagt
     1561 tgacgaagca ttatcaggtt atggcagtga gattgatgtg accatccatg aagacaatag
     1621 tataactgtt gcagatagcg gacgtgggat gcctgtagga atgcatgctt ccggaatacc
     1681 aacagttgaa gtgattttta ctgtgctgca tgctggcgga aagtttggtc aaggagggta
     1741 caaaacttct ggtggactgc acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg
     1801 gctaattgta acgatcgtta gagatggcgt agagtatcag cagaaattta aaaacggcgg
     1861 taaacctgac ggtactttga aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt
     1921 ccatttttta ccagatgata caatattcag tacaacgaaa ttttcatatg aaattttagc
     1981 cgaacggtta agagaatctg cctttcttct aaaaggcgtt aaaatctctc tttctgatct
     2041 tcgtggagaa gaaccagtca aggaaatttt tcattacgaa gaaggaatca aagaatttgt
     2101 tgattatctg aatgaagaaa aagatacatt gacaccagtt gtttattttt caggagaaaa
     2161 agaaggtatc gaagtagagg tcgcttatca atacaatgac ggttattcag aaaatgtttt
     2221 atcttttgtc aataacgttc ggacaaaaga tgggggaacc catgaagctg gtatgaaagc
     2281 ggcgatgacg aaatcttata atgaatatgc gagaaaagtt ggtttgctga aagaacggga
     2341 caagaatctg gaaggtagtg atttcagaga agggttagct gctgtacttt ctatccgtgt
     2401 acctgaaaat cttctgcaat ttgaagggca aacaaaagaa aaacttggta cgccagttgc
     2461 tcgtaccgta gtagataacg tcatcagtga acaaatgggc ttttatttgc aggaaaatag
     2521 cgaaatgagc cagatgttgg tccgtaaagc aatcaaagcc agagaagcga gagaagcagc
     2581 acgtaaagcc agagaagaaa gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct
     2641 ttcaggaaaa ttgacacccg cacaatcccg aaaccctaag aaaaatgaat tgtatttagt
     2701 cgaaggggat tctgctggcg gatctgctaa acaaggaaga gacagaaagt tccaagcaat
     2761 tctaccttta cgaggaaaag tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa
     2821 aaatgaagaa atcaatacga tgatctatac gattggtgca ggagtcggtc ctgagttttc
     2881 tatcgaagac tgtaattacg acaaagtaat catcatgacc gatgcggata cagacggcgc
     2941 ccatattcaa gtattactgc ttactttctt ttatcgttat atgaaaccgc tgattgaagc
     3001 tgggaaagta tatatcgcgt taccgccttt atataaagta tccaaaggac aaggaaagaa
     3061 gcaagtcatt gaatatgcgt ggacagatga cgaattggca gcaatgatta aaaaagtcgg
     3121 caaaggatat atgctgcagc gatataaagg acttggagag atgaatgcgg aacaattgtg
     3181 ggaaacaacg atggatccga cttcccgtac gttgatccgc gtccgtatcg atgatgctgc
     3241 gcaagctgaa cgtcgagtaa ccacactgat gggtgacaaa gtagaacctc gaagaaaatg
     3301 gatcgaaaat cacgtgcaat ttacgctcga agaagatggc agtatcttag ataaaaaaga
     3361 ggatactgaa atctctcctt cagtttctaa tgatttatta gatgaagaac gagcagataa
     3421 aaatgaaaac aatcagttat ttgaagtaga ataggaggtc acctgatgga aaatcgacaa
     3481 gaagtacaag aattaacttt agaagaagtg atgggtgacc gcttcggacg ttattccaaa
     3541 tatattatcc aagaacgtgc actgcctgat atccgtgatg gattgaagcc agtgcaacgc
     3601 cggatattat ttgccatgaa caaagatggc aatacgtatg acaaaggatt ccgtaaatca
     3661 gcaaaatctg tcggaaatat catggggaat tatcatcctc atggtgaccg cagtatttat
     3721 gaagcgatgg tccgcatgag ccaagactgg aagttgcggg aagtactgat cgaaatgcac
     3781 gggaataacg ggagtatgga cggtgatcca cctgctgcga tgcgttatac tgaagccaga
     3841 ttatccaagt taagtggaga gatgctagca gatatcgaga aagaaacagt cgatctcgtg
     3901 tggaattttg atgatacaga aaaagaacca acagtccttc cagctcgata cccaaacctt
     3961 ctcgtcaatg gatcgacagg tatatctgct ggatatgcaa cagaaatccc gactcataac
     4021 ttagctgaag tgatcgatgg cacaatctat atgatcgatc atccacaagc gagccttgaa
     4081 aaattgatgg aatatattcc tggacctgac tttccaacag gagggatctt gcaaggaaaa
     4141 gatgaaatca aaaaagctta tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca
     4201 aagatagagg caatgaaggg taataaacag caaatcgtga tcacagaaat cccatatgaa
     4261 gtaaataaag cgaccttggt gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat
     4321 ggaatagctg aagtacgaga tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg
     4381 aaaaaagacg ccaatgctca aggaattttg aattatttgt tcaaaaacac tgagctacag
     4441 atcaattaca atttcaatat ggtcgctatc gaccatatga ctccgcatca agttggactg
     4501 aaagacattt taagaagtta tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa
     4561 ttcgatttag ccaaagctca aaaaagacag catatcgtag aaggattgat gaaagcttta
     4621 tcgattctgg atgaagtaat cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa
     4681 aagaatttag tcgacgtctt tcaatttaca gaagaacaag cagaagcaat tgtcacattg
     4741 caattatacc gtttgacgaa cacggatatc acagagcttc aaaaggaatc cgaaagcttg
     4801 attgcgcaaa tcacagagtt gaataaaatc ctttcaaatg acaaagagct tttctctgtt
     4861 atgaaaaaag aattgcgaga agtgaaaaaa aattattctt ccgctcgtct aacgacaatc
     4921 gaagacgaaa tcgaagaaat caaaattgat acgcaagtct tagtggcaca agaagacgta
     4981 attgtcagtg tgacgcgaga aggctatgtg aagcgaacaa gccttcgttc ttatagtgct
     5041 tccaaaccag aagaaatcgg gatgcgagaa ggcgactact tgctatactc aggtgaattg
     5101 agtacattgg accatgttct tctgatcaca aataaagcaa atgtgatcta ccgccctgtc
     5161 catgaactac ccgatttgaa atggaaagat gctggtgagc acatttctca aacaatctcc
     5221 aatctgtctg tagacgaatc gattttagct gttttcccat atcaaaaaat tcaagcagag
     5281 aaaacatttg tattcatcag caagaatgga ttgatcaagc aaacacgaat gacagagttc
     5341 gaaccatggc gcacctataa gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa
     5401 gatgaactga ttgctgtgta tctgaaagaa gatcagccag gcttagatgt tttcttagta
     5461 acgcatcaag gaatgggttt gcgctatcct ttaacagatg ttcccgttgt tggagcaaag
     5521 gcagctggag tgaagtctat gaatttgaaa gatggagata ggattatcaa cgggctgctt
     5581 gtgttagcag aaggtgatac gccgatcgta atcgttactc agcgaggagc tgttaaacgc
     5641 atgcttgctc aagaaatcag ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt
     5701 gagctgaaaa aaaatccgca ccgtattatc tatatgtcgg aaggaagatc aaaagaaata
     5761 accttgatga atcaaaaagg gcagcagtta acgattgacc caactgattt ccctataggt
     5821 gatcgaacat cgaatggatc atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa
     5881 gttatcgatg ctcctgagat tttcatcgag tcgtaactgt tgtagtattt taatgaagag
     5941 acgccatctg tattaataca ggtggcgtct ctttttcatt catttttaat aaatccttat
     6001 acgtttgcaa aaaaagatga aagtgttcac aatgcttaaa tgttggtatg tcaacgttga
     6061 tttaaatata tgtaaataaa acataaaaat tgttaaataa aaaacaagaa aacccttgca
     6121 aagatagggt gctgtgttat actaatcacg taaaaactgt tacataaaac agaaatttag
     6181 gaggtccata caatggagca atggaatgga tttaaaggta ccaaatggaa agaaggcgta
     6241 gacactagag actttattca aaataactac actgaatatc gcggtgatga cagctttttg
     6301 gaaggcccaa cagaagcaac agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc
     6361 caattcgagc gtaacggtgt ctatgatatg gacacagaaa tcgtatcaac tattacttca
     6421 cacgatgcag gctatttgga taaagatttg gaaaaaatcg tcggtctaca gacagacaaa
     6481 cctttgaaac aagcattcat gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa
     6541 agcaatggct atacaccaaa cgatgaattg acacatattt tcactgactg gcgcaaaaca
     6601 cataatcaag gtgtatttga cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa
     6661 atcattacag ggcttccaga cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt
     6721 gtggcattgt atggaatcga ctatttgatg gaacaaaaga aaaaagatca tgatatgact
     6781 ggttatcata caatgagtga agaagtgatt cgtttacgtg aagaaatcac tgaacaatat
     6841 cgtgcattag cgcaaatgaa agaaatggct gcgaaatatg gctatgatat ttctaaacca
     6901 gctgcaaatg caaaagaagc tgtccaatgg ctatacttcg gatatttagc agcaatcaaa
     6961 tcacaaaatg gtgctgcgat gtcaatcgga cgtatctcag cattccttga tatctatatc
     7021 caacgtgatt tggataatgg agtaatcaca gaaaaagaag cacaagaatt gatcgaccac
     7081 ttagtaatga aattacgtat ggtcaaattc gcgcgtacac ctgaatataa ccaattattc
     7141 tcaggattcc ctatctgggc tactttatcc attgcaggta tgggattaga tggtcgttca
     7201 ttagttacaa agaatgattt ccgtctgctt catacattaa caaatatggg accttcacca
     7261 gaaccaaact tgactgtttt gtattctgaa caccttccag aaggattcag aacatatgct
     7321 agcagaatcg ctatcgaaag ttcttcgatt caattcgaaa atgataatca gcttcgtgaa
     7381 caatggggtt ctgatgattg tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa
     7441 gacatgcaat tctttggcgc tcgtgctaac ttggctaaaa cactattata tgccatcaat
     7501 ggcggagtcg atgaagtgac tggcgcccaa gttggaccag aatatcgtcc aatcacatca
     7561 gaaatactag actttgaaga agtgaaacat aacttcatga atatgatgga ttggttagca
     7621 gaactatatg tcaatacgtt gaacatcatt cattatatgc atgataaata ttcttatgaa
     7681 gcagctcagc tggctttgat ggattctgac ttgcaacgca catttgctac aggtattgct
     7741 gggatctctc atgcggcaga cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc
     7801 cgtaatgaaa aaggaatagc agttgatttt gaagtggatg gcgacttccc taaatacgga
     7861 aatgatgatg accgtgcaga tgaattagtt tcatggatct tggaatactt catgggacaa
     7921 atcaaacgtc ataaaactta ccgcaactca aaaccaacaa cttcattatt gacgattact
     7981 tcaaatgttg tttacggtaa aaatacagga aatactcctg atggccgtga agctggaaaa
     8041 ccacttgcgc caggagcaaa tccaagttac aatgcggaac aaagtgggtt attagctagc
     8101 ctgaactcaa cagctaaaat gaattacggc tgtgcattgg atggaatttc aaatacccaa
     8161 acaatcaatc cttcaggtct tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat
     8221 gtcttagatg gatacttctc aaaaggagct tatcatttga atgtcaatgt attcagtaat
     8281 gatttattgc gtgatgcaat ggaaaatcca atgaaatatc cgaacttgac gatccgggta
     8341 tctggttatg cagttaaatt ccgtgacttg acaagagaac aacaattaga tgtcttgatg
     8401 agaacttcac acgacagaat gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga
     8461 aaggacgttg aatagccatc gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa
     8521 cgatttacag gggcaggact tgtatgttcc gtccctgtaa attttacaca taacgaagcg
     8581 gcaattgcga aaaattttgt tcagaaagct tcgcttcgac agctaacttg tacttaaaat
     8641 gaaatcaaag aaaagatgtg aacttaaatg gaagaaaaaa caatcggcta tgttcattct
     8701 atcgaaactt tcggttcagt agacggacct ggactgcgct ttgtcgtgtt tatgcaagga
     8761 tgccgcatgc gttgccaatt ttgtcacaat ccagatactt ggaatattgg cggagggaaa
     8821 gaatacacag cagatgaact tttagataaa gcagaacgct tcagacctta ctggggagat
     8881 aaaggtggta ttactgttag tggtggagaa cctctattgc agatcgattt tttgatcgaa
     8941 ctatttaaaa aagcaaaaga gcggaagatg catactacat tggatacatg cggaaaacct
     9001 tttacgtatg aagaaccttt cttttcacgt tttcaagaat tgatgaagta tacagattta
     9061 ctgttatttg acatcaaaca catcgataat gaagagcata aaaaactaac gcattgggac
     9121 aatgaaaata ttttagaaat ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt
     9181 cgccatgtac tagttcctga gcgaagtgat tatgatgaat atttgatccg actagacaac
     9241 tttattcaaa ccttgtcaaa tgtagacaga gtagagattc ttccttatca tacgatggga
     9301 aaatacaagt gggaaacact tggtttgaaa tatccattag aagggatcga accacctaca
     9361 aaagaacgtg tcgaaaatgc taaacgattg cttcatacag aagagtatac aaaatattta
     9421 acgcgatagg tcgcatcgga aagaagataa gtattgcttc tttccgtttt tttgctatta
     9481 ttaaatagga gaaatcacaa aggagcgaat gacatgtcaa aaattttagt ttttggtcac
     9541 caaaatccag atacagatgc aatcggggca gccattgcgt tttcacactt gcaaaatgaa
     9601 ttaggtaaag aaacagaagc agttgctcta ggtacaccta atgaagaaac acaatacgca
     9661 ttagaccact ttcagctatc tgcaccacga gtagtagagt cagctaaagc agaaaatgca
     9721 gatgtcatgc ttgtggatca taatgaattc caacaaagta tttcagatat cagcgaagta
     9781 aatattttag ctgtagtaga ccaccatcgt attgcaaact ttgaaacagc taatccattg
     9841 tactatcgtg cagaaccagt tggatgtaca agtacgatta ttttaaaact atataaagaa
     9901 aaccaagtaa caattcctaa agaaatcgca ggaatcatgc tctcagctat catttcagat
     9961 acattgttgt tcaaatcacc aacatgtacg cctgaagatg aagcagctgc aaaagaactt
    10021 gcagcaatcg ctgaagtcga tctaaacact tatggacttg aaatgttgaa agcaggaaca
    10081 aatctaagtg ataaatcagc tgcggttctt cttgatttgg atgcaaaaag tttcccaatg
    10141 ggcagttcca acgttcgtat cgctcaagtc aatacagtgg accttaatga agtgatggat
    10201 cgacaagcag agctagaagc agcaatggaa gatgaaatga aagcaaatgg ctacgatctg
    10261 ttcgtattag ttgtcacaaa catcttagac agtgattcag aattacttgt tgctggtaaa
    10321 ggaaaagaaa aagtagaaga agctttccaa acaactttga ccaacaaccg tgctttgctt
    10381 aaaggtgtcg tttcacgtaa aaaacaagtc gtacctcaat tgacggctgt atttagctaa
    10441 tcaaatggta gtatgaagca ttcaaaatga atggataagc gattgatata aaaagaaaca
    10501 tagaacatac taaaggccga agaaatggag gagaataaca gtcaattatg actcttgttc
    10561 ttcctccttt ttacgtaaaa gccttttaag taaaataaaa cggatagaaa agagaacata
    10621 tatgaactat tattcaaaaa ataaggaaaa cttttatcaa aaactgaccg atgatccgct
    10681 tttttcttca ttgaccgatt atctatatga acatcgagaa caagaaacga ttctaagaga
    10741 attgaaaaaa gaattttcgc aaaataaatt ttcccatttt cttgatttat tgatcgatgc
    10801 aggattaatc aagagggaag aacgaagata tcatttgaac tttccaatat ttgatcctaa
    10861 agattacttg caacaagcca cttcggctgc tgaaaccata gctgaacaat tgaagagact
    10921 ctctgttgat gaacaaaaat tagcgatggg agaagttatt tgggcgtatt gtttcgaaga
    10981 tgaacggaaa gaagcgtatt tttatggagt aagaaacagc cgagagacag aattgctgag
    11041 gacaactgca ggaaatgaga agtaccgttt tatcacactt tcctctatag aacacttccc
    11101 gcttactttg gctaattact tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt
    11161 caaagaatta gccgaactta ttggagacgt aaatgaatcg tacttttttg atcaaataga
    11221 agttatcgtt gacaggatac gtaagaataa atataaaaat cgccggccta gtattttcca
    11281 tcagtctcta cttgtgacga atactataaa ggaagaagaa tcattcacat tagaattacc
    11341 tattgtcgaa aaaaacaatt ttgagattga attaccaaca ttggatcctt cattgacaat
    11401 ggaggaaatg gcctttctta aacgacaaat cttttctgag ctgagcaaga aatttatacc
    11461 gcatgcattt tcttatatca aagaatatgg aacagtttta gtgagtaaaa cctaagagag
    11521 cagtaagcaa aaattatact gactcttgca ggaggagcct ttctcttcct ttatggttaa
    11581 aagaattatt tgagtatttt gcctatctac cacctattag attcactaca tggtctggaa
    11641 acaaaggaat gcaaaaaaat ggtgagaaat gtggaatgac aaaggaagta gtgataagaa
    11701 aagttagatt tttaaataat caatattatg attcagtcaa gtatggcatt ttatgggaag
    11761 aactagctga ttagattagg aagtccattt gataatccag aatcactaag aaacgaatgg
    11821 cgagtcatta ataatttatc caatcttggg ttattctttt agattgaagt gtttaaaact
    11881 aaaaaagcat ttatgaagtc gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt
    11941 ttttctttgc cacataaata cacctagact gacgatgaag agcatatcca acagatttcc
    12001 tgcgacttgg atcgtcgaag ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa
    12061 caagctgtaa ataaaacagg tgacaggaac taatcgaaag aagagcggac atttcaattg
    12121 gctaaaaatg agtgaaaaaa taaaataagt aaaaatggat aaaatcacga ctaaaccatt
    12181 catgataaag aagaaaagcg tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga
    12241 aaacgtttta aatgcaatca atacgcttga agcttcgttt aacgtaccag gatgtcgatg
    12301 aaaaaaatag gcaccgaaat aaacagaaaa cagtaagccg caaagagaag acaaaatcaa
    12361 aaaaaacgga ttttcttcgg cttctgtgat atcagaatga tccaaaagca accagcctaa
    12421 tgcactagaa ggcgcatgtg aggtcttaag gtatgaataa catagaaaaa ataagatcgt
    12481 caaagttgca aaaaaagagc ctaaatgtaa aaaattagtg gttctacgtt gaatttccat
    12541 aatatctcca aattaaattg ataagtatat aaagtataat aacaaattga tgagtatatg
    12601 tcgatgtttt ttaatcaaaa taataaataa aaactgtcta acatttaaaa tttaatttag
    12661 atattagaca gtttttaagt ttgtgagtta ttttccttgt cgttttctca cttttttagt
    12721 ccagaaacca cctgaaatat attttggctc ataagaaatg ataaaagcac gctcttcgat
    12781 ttctttgatc aggcgataca gttcacgttc gctttttcta ggagaaagta tttcgagaac
    12841 tagccgctca ccttccagcc ccattgcaga agtctgtgtc acaccataac cgtgttctct
    12901 cagtataccc ggcaaacttt tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat
    12961 gatgtatcct aatgccaatt tgtcttcaat caaaattccg acactgatac ctactgcgta
    13021 acctaacgca tagacaaata aattcagtgg attatctaaa cgatctaaaa caagggataa
    13081 gccgacgata taaatcgtga tctcgaccat actgacaagt ggagcaatca gtcgatagcc
    13141 tttcattgtc aacatgaaac gtaacgtgtt cagagtgata taagcaaaat taatgaggaa
    13201 gatcatcccc aacatttttg gatcaatcat ttgtaaaact cctttcattt atataacaac
    13261 aatatcaaaa aaaatcgtaa gtgaagaaaa attgaaactt tatacataca aaaaaatgat
    13321 aaaatgatta taatagaatt ataaagatag gttaggtgat taaaatgaaa acggtcaatc
    13381 gtgtgttttg tgccagccac gtgctaagtc tccttctcag tatatactta ggtgtctatt
    13441 cgattcaaca gccaattgag gtagagaaca ttgtttttca gatgtcgctg acagatggtt
    13501 taattttaag cgtttttttc tttttgatac tgtttattgg taatattttc ggtagcgttg
    13561 ccagtttttt gaatttttct atttatccac tcctttcgct agcattagga gtggtgggac
    13621 tttgttctct gtgcttattt cccgagccat tcagtcctgc atttatatta tttggtacac
    13681 tgaacctgtt ccagacaact gttggagcat ggttgctatg gcgatcagga aatttaatga
    13741 aaataggtga gtaacaattg aatccaatta tattgaaacg ttctgtccat catattccag
    13801 tgttagaagt tgttctgaaa gaattaagag aggaacgtat tcccgttgtc atctattatc
    13861 acggatggca gacaagtaag gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg
    13921 gcattcgtgt gatcctgcca gatgcgatga atcatgggga gcgcaaacag ccggtttctt
    13981 ctattccatc ctatacattt tgggatagta tctacggaaa tctgtttgaa tttgacacac
    14041 ttatcaagca tttagaaaaa agaggtcttc tacatgatca gcttgcagtt ggcggggttt
    14101 cgatgggggg aatgacaaca tgcgccttgt tggctaaaca tcctcatata gtagcgggcg
    14161 tgtgtttgat gggttcccct gcacctttgg aatatggaaa agaaatcgta aggcgcgcat
    14221 cggaatataa ttatgtcgta ccaaaagact attttgattt gatttcatgg gttaagagct
    14281 atgatttgtc attgcagccg gaaaagttag ctggacgtcc tctgttcttt tggcatggac
    14341 aagctgacga aaaaatccct tatatacaaa cggaacgatt tgtagaaaaa aatcggcata
    14401 agtcttacgg aaaagaaatt gtttttcgtt cctcaaaaac acagcgacat atggtacaaa
    14461 taccactaat ggaagaagct tccacttttt tagcaaaaaa tttgataaaa aagtataaat
    14521 aattgaaacg gaaaatcact catgacgata gaggattttc cgttttttat tttgggctat
    14581 aataaactct gttaggaaaa tttggtataa ttgatagtaa gaatgacaac atgttagaaa
    14641 ggaagagagc atgcccactt tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac
    14701 ggtctctcga gtcatcaatc atccggaaca ggtgactgac gaattgaaag agttagtttt
    14761 ctcagcaatg gctgaactgg attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa
    14821 tcggtcgcag atcatcaaat tatttatact ggaagaaatc gacacaactg agccctatta
    14881 catgaatttg ctgatgggaa tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt
    14941 agttacaaat gatgccttcg acgtcggttc ttgtgatgga tacatcatca caggtgtcag
    15001 aaaaaaagac ttcgattgga tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa
    15061 ttctgaagga tatgattatg tagacagcga taatgaatat ggtacagcaa aagcaacgca
    15121 atatgcctta gaaagagatt atcaaacgat tatttatatc ggtatcgaag tggacgagcc
    15181 ttttgaaatt tcaagggaaa ctggttattt atctgtgatg aaggaagttg atcttcctag
    15241 taaaatcttg cggtttccaa atcattcgac agaagtagaa agatatatga cagaaaactg
    15301 ggcagatttt acagataaaa cttgttttgt atgtagttca gatcggctag cactaggagc
    15361 agtaagaggg attataaatg ctggaggaca cttgcccgaa gaatttggtg tgattggatt
    15421 tgatggggta tttttagacc aaatcagttc acctaaattg acaacgatca agcaagatat
    15481 tatacgtatg ggtgaagtat gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc
    15541 gagtcaaggt tatcgtcgtt tttttcctga actagttatc agagagacaa cgaattaagt
    15601 cttattacta tatatatatt tatgaaattc atcgctattt tcttaaaata atcagcgcat
    15661 tgctactgaa aacaatgaat ctgtgctatt attgagaaaa gttccatttt tgagaaggat
    15721 ggtttgaatg aaagaaatca gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt
    15781 aggcgtaaaa ttaaccaacg acaagaagcg agtattattt gacggacaag aagatctttt
    15841 gactgaacag aatcagtccc cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa
    15901 caccgagaaa atccgtcgca agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa
    15961 ggaaaatttg gaaaagcata aaggaaaatt accggattat accggccatt accaatcaga
    16021 aatcacatcg acaggacgaa aaaaactttt tggtgattcc tctcgttctg tcagacaaag
    16081 tgaaacaagg acagagtcca agcatactac acgaaacgca tctgttgaca cgaaggtgcg
    16141 ggagcgttct tattttgttc ctaaatatgt acccgcatcg attatcccag accctagaga
    16201 aagcaaaatt tctgaggatc atttaatcga atcgatgaaa aaaacggatg actcttatct
    16261 gttgttcgat acagaaccga cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg
    16321 aacattcaat cgaacaaaaa cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa
    16381 aaaaagccat ggtatcttag aacgctcatt acaaggattg atcgatgatc aaggaaactc
    16441 tttgggagaa aatagctatt ttaaataagt gatatacaaa tttatattcg gctacagttg
    16501 gaaaggaaac gctgcggcgt ttccttattt tatactctat ccgaaagttt atatcgtatc
    16561 ctcaaatttt tttgagttta agcgaatttt ttctcccaat aagtaagaaa ataatggcaa
    16621 aataaaaaaa caaactattt tcgcagatcg tttgcaaaaa tagtttattt ttttataaag
    16681 aagattttac tgaataatat cattttagtt tacataatga tatttctgta aattaaataa
    16741 agtagtgaaa aataagaatc agtgctctaa ctgttcaaga ggatcaagtg gttcaaatgt
    16801 ttctgtagtc tgttttgctt ccaattgggg caaaacagat ccaatttcca gatacttcac
    16861 tttattcaat gtataagata gaatagaaca gctaagtaat gtatacagaa aatgtgtgag
    16921 atccaaataa agcaaggaga caactaaaat accaaaagta atcaagaaaa gacttttgcc
    16981 aatcttaata tgaaaatatt tttcaatggc ttgataaatg atatctgttc cgcctgtgct
    17041 tccgccgaat cgaaagacga gaccgcaacc aatgccagaa agaatacctg tcatcaaact
    17101 attgatcagt agactatcta cagcaaaatg tatatgaagg ctctcccata aatgtaaaaa
    17161 gaaaactagc gcaaatgttc cgacgatcgt ccggagaaaa gcgtgttttc caaaaagaaa
    17221 caatgagata agcaataaag gtaaattaaa aagcagtgaa gtgacagaag gagcgaatcc
    17281 aaaaagatta tgcaaaagca aagcaatacc agtaacccca ccatctgcca gcttagatgg
    17341 aatattgatt cctgtcacag ctaatccgta gattcctgcg cctaaaatga ttccgcacaa
    17401 ttctgtaaat aaatttaatt ttttcatttc tagcctcttt tcttttgaaa attttcagca
    17461 acttctttat tatactgact tatactctta cttcaagagg aaaatgaaaa aaaattattt
    17521 ttggctgttt ctttttattt atcaaataag gagtggaaaa atgcgagcag cagacttatt
    17581 gacaattctt acaaaatcac ccaaaaaaac agagtatcaa ggagtttttt tggctcaaga
    17641 acaaaaactg gtgcctgtga caatggtcca gataaataaa gcaaatcaat ttatcttttt
    17701 atttgaacac ggcaaagcac cacttgctat gaaagacatc tttctttttt tgatgaagaa
    17761 ccgtgagaaa gctgtttatt atcagtcaaa agaagaactc attcctcttt atggagtgaa
    17821 agagatagaa aataaagtgg tgatataagt aaaaataaga aaacaagacg tttatgatgt
    17881 caaaacaacc attttttaca tttttggcaa aaaagtgtca tgatacagta taatattcgt
    17941 tgaacaggcc aataaaggtg ttctccccca cgagatttct actttattgg ctgtttcttt
    18001 ttattaaaat aaaagttgtg tgtgtaaaaa agatatagct gattccaagt gacaatttcg
    18061 tatagatttt tatggtattc gtagtgaaaa aacgagaacc aaaaagttga tccgactttg
    18121 ttgcttggat tctttttttg tcagcatatt tagaggtatt tttttctttt ttttgttcgt
    18181 gctgtaaggt taaggcaaga caagttagtt gggaaaaagg aaagaaatgt ttatacttaa
    18241 agaataacag aaaggaaggt cgcatgaata attttataca aacattaagt gaaagaaaag
    18301 gcgagttgct ggaagctgca tttcaacatc tgtccatttc tctcatttct ttgctgattg
    18361 cagcactgat tgctattcct ttagcgattt gggcagccaa tcataaaaaa atggcagaag
    18421 tcttattgca gatcacgggt gtgctccaaa cgattccttc acttgcctta ttaggtttac
    18481 ttatcccatt tgtagggatc ggaactgttc cagcattgat tgcacttgtt atttacgctt
    18541 tgcttccgat tttccaaaac acgtatattg gtctagctga aattgacccg tctattgaag
    18601 aagcagcggt agcatttggt atgtccagaa tgagacgctt gctgaaagta gagcttccca
    18661 tcgcattacc tgtcattatc tcaggtatcc gaactgcctt agttttaatc atcggtacgg
    18721 ctactttggc agcattgatc ggagcaggtg gattagggac atttattttg ttaggtattg
    18781 accgaaatac acctgttttg acattgattg gtgcgatcag ttctgccttg cttgcaatta
    18841 tattcagcgg actgattcgc tggttgcagc ataagaaacc tcgatattcc atcattactt
    18901 tagcagcagt ggtgtgttta tttggcggcg tttctttata tcagtctgcc ttgtttaaaa
    18961 atgaaaccat cacgattgct ggaaaattag gagcggaacc tgaaatcctg atccaaatgt
    19021 ataaagaatt gattgaagac aacacaaaaa cagaagttga attaaaaccg aattttggca
    19081 agacgagttt tttgttcaat gcactggatc aaggtcaaat cgatatctat cctgagttca
    19141 caggtactgt cctagaaagc ttggtgaagg tacctgaagc aatgaaaagt gctgatttga
    19201 caaaagaaaa aacatatgac gaagcagaag aattattgaa taagcaattc gatatgacat
    19261 tattaaaacc aatggcttac caaaatacgt atgccttggc agtcaagaga tcttttgcga
    19321 acgaacatca acttaaaaag atttccgatt tggcagctat ctctgatcag atcaaagcgg
    19381 gattcacact ggaatttatt gatcgttcag atggatataa aggaatccaa gaagcatatg
    19441 gcttgaattt ttcaagtgta caaagcatgg agccaagttt gcgttaccaa gcaatcaata
    19501 atggagatgt caatattgtg gatgcctatt ccacagatag ccagctcaag gagtacgatc
    19561 tagtgactct agaagatgat cgagggttat tcccagctta tcaaggcgca ccgctgatga
    19621 aaaaagattt tgctaaagaa catcctgaaa tcgttaaagc actgaataaa ttaagcggaa
    19681 aaataacaga ggatcaaatg attgatatga attaccaagt caatgtcgaa aagaaaaagc
    19741 cgacagaagt agcacgaatc ttcctgaaaa atgaaggatt agtaaaggag gaagatcagt
    19801 gactaaatta atcgagttca aacatgtgca aaaaaagtat gatggaaagt atgtcatcga
    19861 tgacctaaat ctggcaatca aaaaaggaga aatctttgtt ttggttggtc cttccggtag
    19921 tggaaaaaca actactttga aaatgatcaa tggattatct aaaccatcag caggagacat
    19981 ctacttcaaa ggaaaatcgt taaatgaata taatctgcag aaaatgcggt ggaatatggg
    20041 atatgtgctg cagcaaatcg ctttgttccc gaccatgaca gtcaagcaaa atattgaagt
    20101 catccctgaa atgttaggtt gggaaaagca gaaaagagca gaccgtgtag atgaacttct
    20161 tcaaaaagtt ggcttatctc ctgatattta tcgagatcgt atgcctagag agttgtctgg
    20221 aggagaacag caacggatcg ggatcatccg agctattgct gcaagcccag atgtgatttt
    20281 gatggacgag ccatttagcg cattagatcc gatttcaaga aactcattac aagaattggt
    20341 tttgtcctta cacgaagaat tgggcacgac catcgttttc gttacgcaca atatggaaga
    20401 agcaatcaag ctaggtgatc gtattgcatt tatgaaggat ggagaaatta tccaatgtga
    20461 cacaccagag cagttattga tgaatcctaa aaacgactac gttcggcatt tctttgatga
    20521 accgaaacag acgaaagagt ggcgagtaga agatttagtc gtcaatggat atttcttaaa
    20581 tgaaatacca gaaaatgctc gtcaacaagt ttgctttgat acgcctatga aagaagtata
    20641 ttcgcttctt tcggtgtatc caagcattgt gatagtagaa aagcaaagaa gtattgggtc
    20701 gctgacaagt aaagaaatct ttgctttttt aagtcgagag gaggaaggaa atgaaaacat
    20761 atgatgcaat catcatcggt tcaggtgtga gcggcttaag tgctgcttat ggattgaaag
    20821 aagcaggtaa aacagtacta gtggtcgaag aagatctatg gggcgggact tgtcctaacc
    20881 gtggatgtga tcctaaaaaa gtcttgttaa gtgcagtaga ggcacgtaat cgggtaaaac
    20941 aattgagtgg aaaaggtttt aacgagatcc caactgccaa ctgggaagaa cttcaaaaat
    21001 tcaaacgaac atttacggat ccagtcccag aaagccgaaa aaaacaatta gcagaagctg
    21061 aaatcgatca tctttcagga accgctcgtt ttttggatga ttcatctatt gaagtcaacg
    21121 aagaagtctt ccatgcggat tacttagttt tggcaaccgg gcaaagacct acgatcttac
    21181 cagtggaggg aaaggaatac ttgaaaacta gtgcagactt tttatcattg cctgtactgc
    21241 ctaaagaaat catttttatc ggtggtggtt atattgcttt tgagctagct acgattgcca
    21301 atgctgcagg gagcaaagta accattgtcc atcataatca acggccatta aaagaatttg
    21361 aggcgtcttt ggtagaggaa gccgtccatc aaatggaagc atctggcatt cagtttgctt
    21421 ttggtgttga aacccaaaag atcatttcag aaggtactcg ctatcgttta gtcggaaaag
    21481 agacggaatt agtagcagat atgatttttt gtgcgactgg gagacagccg aatacagaat
    21541 ctttagcact tgagcaagcg aatatcgtct ttgataaaca cggaattgca gtcaatgatt
    21601 atctgcaaac gagcaatcca aaaatttttg cgtgtggaga tattgtttca aggaaaaccc
    21661 caaaattgac accagtcgcc acttttgaag gaaattatgt agctaagcgg ataacggatg
    21721 caacaagcga acctatcaaa tatccgatta tacctacgat cgtctacgcc agtcctaaac
    21781 ttgctgaagt aggagttaca aaaagtcacg cctcttcgtc agatcaagtg gtagagatgg
    21841 atcttaccag ttggttcacc tatcatcgtg tcaatgaacc agttgctaag gctgaattga
    21901 cctttgacca gcaaaattat ttgattggtg ctgcagtgat tagcgagcaa gcagacgaat
    21961 tgatcgatga tttgacactc gtcatcaatc aaaaattgac taaaaaagag ttggatagct
    22021 atatcatggg gtatccgaca ttggcaagtg atctctccta tttattgaaa taatcagcat
    22081 gataactaaa gcgcttgttg ctttttcctt cttgtgctac actttaaaaa atgaatacct
    22141 gtttctgggg atgtcgtaga cgacatgaga gtaagttgtg aacttagacc cattgaacct
    22201 gtcagttaat actggtgaag ggagaaacat agcagacgct tgaagcgggt tactatgtcg
    22261 tgcccatttc aagcgttttt tgttattcat attagaagaa aggaggtaac aaaatgaaga
    22321 aaaatcttta tccagcagtc agtatggcat tcattttaat tatttggcag attgttgtca
    22381 tagtttggaa aatacctgcc tttattttac ccggacctgc tgctgtattt tctgcattag
    22441 cggcagatgc acaagtatta ctttcccatt ctgtcacgac attaaatgaa gcagtgcttg
    22501 gtttggtaat agctagcgtt ctttctttta ttactgcctt ggcaatggat tattggaagt
    22561 ttttagaaat gtctttttat ccgttactag tgatttcaca aatgttgcca atcatggtgt
    22621 tagggccatt acttacccta tggtttggtt tcggtatgct tccaaaagtg atccttgttg
    22681 tcttgataag ctattttccg atcgtagttt ctttttcgga tacattgagg aaagtatcaa
    22741 aagagcaaat catctttttg aaaacgatgg gggcagatac agggaagatt tatcgaatat
    22801 ataaaatacc agcgggttta tccggcttct tttccggttt aaaagtggct gctacttatt
    22861 gtgtcagcgg agcagtcgtt ggagaatggc taagcgctca agcaggctta gggtattaca
    22921 tgatacgcgt aaaaaacagc taccagatcg ataaagtttt tgctgcgatc atttgtgtca
    22981 ttctattgag tttattgctg aatggcgctt gttcattatt gaaaaaaggg tattatcgta
    23041 tgctttatat tttttaagag aaggggatca aatgaaaaaa ttatggcttt tattaccgct
    23101 actgttgctt ttatccgctt gtggcactgc aaaagaaaca agtagcaaac aagaaataaa
    23161 ggatttgaaa aaagtgacac ttgttttgga ttatgtaccg aatactaatc atactggcat
    23221 ctacctagca aaagaaaaag gctattataa agaggctggt ttaaatgtcc aaatcatcga
    23281 accaggagat aattcgacaa gtattggtct agtaggtgcg gacaaagcac aatttggtgt
    23341 aagttatcaa gaagatgtca cttatgctca cgcagatgga cagaatatac cagtcaaagc
    23401 aattgctaca gtgatcaaac ataatacttc tggattcgcc accctatcag acagtaacat
    23461 ccattcacca aaagattttg aaggaaaaac atatgcaggt tggcaaagtc caagtgaaga
    23521 agctgtctta aaagctgtga tggaaaaaga cggcggggac ttttcaaaac tgacaatggt
    23581 aggaagcaat ggagaagggc cagaaagctt aggaaaatcc tcagatatcc aatggtactt
    23641 tgaaggttgg gatatgatca aagcaaaaga agcgggtatt gaactaaact atatcccatt
    23701 gaaagaatta gatgaacggt tagattacta tactccggta attattacga atgatcaact
    23761 gatcaaaagt gatccagaat tggtacagtc ttttatggat gccacgaaaa aaggctatca
    23821 ggaagctatt aaggatccaa atgacagtgc taaactgttg caaaaatatg caaaagaaaa
    23881 tgatcggacg tttttagaag aatcacaagc attcttgtca aaaaattata cggatgatcc
    23941 tgagaattgg ggattgatgg aagaaaaagt ttggcgcaat tatacagctt ttatgcagga
    24001 aaacggattg atcaagcaag atgtaccttc gcaagaacta ttcactaatc agtttataaa
    24061 ataggagtga tagatatgtc aatccaaatc agccacttga atgtagtcat tgaaaacctg
    24121 aaaatattag aagacatcca tttcactatc cctaaagata cctttacatc gattgtcgcg
    24181 ccaagcggag ccggtaagtc aacattgctg aaaactttaa caggagtcca gcctatgact
    24241 tctggaacga tcaaagtaga cgatcaaaaa gtaactggtc tccatacagc atttagttat
    24301 atgccgcaag aagatatgtt acttccttgg ctgagcgtct atcaaaacgt tacgttatat
    24361 caaaaaatca atcatctaac aatcaatgaa gaacaagtaa gggattatct ggatatcttt
    24421 gggttaaacg gctatgaaca ttttttgccg gaacaattat ctggcgggat gaagcagcga
    24481 acggccttgt tacgaacaat catgaatcct tcttcttact tgcttcttga tgaaccattt
    24541 ggagcgttag atgctatgac cagagggcag atgcaagact ggatgttgaa gctgcctaaa
    24601 aaagcaaagc ggactacttt acttgtgacg catgatatcg aagaagcaat ttatttatca
    24661 gacaggattc ttgtgctttc cgcccgtccg gcacatgtga ttgctgagat tcaagtgcct
    24721 gaaaaacgac ggtcacgtga atggctactg cagcaatcag agttgaagca aacgatttat
    24781 cagttattgg caggtgaatt agatgttaag tgatgctcat tgtcatttag atgggagtca
    24841 gagtttagca ctccttcagc aggaacacac gattttgacg atcatcaatt gtgattcgcc
    24901 agaagaatgg aaggaaaaca gacagcttgc agcaagtaag actcaagcct taagttacgg
    24961 tatccatcct tggaaagcag atagctatac gtttgaacaa gttgagcctt ttttgaaaaa
    25021 agcaaggatc atcggtgaaa tagggttaga taatatttgg acgaatgtac cgatgactac
    25081 acaaaaaaaa gtatttgagc gacagcttgc ttttgcagcc ataaatgaga aacctgttgt
    25141 actgcacaca aaaggatgcg aaaaagagat cttaaattat atccaagctt accctaatca
    25201 ctatttgatc cattggtatt cttctttgga ctatcaacaa gactatattg atttaggctg
    25261 ttatttcagc attggtcttg atttgaaaaa gaatccagca gtctggcaac tagctaaagc
    25321 agtcccaatc gaccacttac ttattgaaac ggatggacta catgcagcag agtgggcttt
    25381 aggggggatt ttttcagaaa aagattatgt ctcactgctt gaagaacagt taaagacaat
    25441 cgctgagatc aaagagctat cagttgaaga agttaaaaaa aatacttatc aaaacttgga
    25501 acgtttcatt agattaggta aataaaaaag agatgtgaca caactagtgt cgcacctctt
    25561 tttggctttc tttgtataaa agtaaactgt ttgattaata aatgaatctt agtaccaacc
    25621 gtttgcttcc cagaatgctt tcgccgcttc ccaagaacca taacgtgaag taacatattg
    25681 ttctgctact ctttcttggt ttgcagcaga gtagtcacca tttaagtaag aagaatctaa
    25741 ttggtaacgt ccgatgtaac gtccgttcgt tgctgtgtaa gaaccgcttg attctttttg
    25801 tgcgatccat tcttttgcag agcttgtgct tgctggagta gcagcagttt ctgttacagc
    25861 aggagcaggt tgtgtttcag caacaggcgc tgcttgtttt tccgtttgaa ccgtttcttg
    25921 tacaacaggt tgttcagctt ggactggtgc ttcttgaacg gctgcttgag gttcagcagc
    25981 tgcttgtgca gagccttctg ttgggattgt taattgttgt cctgagta
//
LOCUS       DACEPD010000035        25900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.035, whole genome shotgun sequence.
ACCESSION   DACEPD010000035 DACEPD010000000
VERSION     DACEPD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.035"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(85..2352)
                     /gene="gshAB"
                     /locus_tag="IW797_001354"
     CDS             complement(85..2352)
                     /gene="gshAB"
                     /locus_tag="IW797_001354"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ1224138.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(2539..3537)
                     /gene="ftsY"
                     /locus_tag="IW797_001355"
     CDS             complement(2539..3537)
                     /gene="ftsY"
                     /locus_tag="IW797_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ1224139.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(3549..4358)
                     /locus_tag="IW797_001356"
     CDS             complement(3549..4358)
                     /locus_tag="IW797_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1224140.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(4367..7948)
                     /gene="smc"
                     /locus_tag="IW797_001357"
     CDS             complement(4367..7948)
                     /gene="smc"
                     /locus_tag="IW797_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ1224141.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(7968..8654)
                     /locus_tag="IW797_001358"
     CDS             complement(7968..8654)
                     /locus_tag="IW797_001358"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ1224142.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(9002..10783)
                     /locus_tag="IW797_001359"
     CDS             complement(9002..10783)
                     /locus_tag="IW797_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1224143.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(10809..11723)
                     /locus_tag="IW797_001360"
     CDS             complement(10809..11723)
                     /locus_tag="IW797_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1224144.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(11750..12712)
                     /locus_tag="IW797_001361"
     CDS             complement(11750..12712)
                     /locus_tag="IW797_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1224145.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(12712..13656)
                     /locus_tag="IW797_001362"
     CDS             complement(12712..13656)
                     /locus_tag="IW797_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224146.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(13657..14661)
                     /locus_tag="IW797_001363"
     CDS             complement(13657..14661)
                     /locus_tag="IW797_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224147.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(14929..15171)
                     /gene="acpP"
                     /locus_tag="IW797_001364"
     CDS             complement(14929..15171)
                     /gene="acpP"
                     /locus_tag="IW797_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ1224148.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(15293..16294)
                     /gene="plsX"
                     /locus_tag="IW797_001365"
     CDS             complement(15293..16294)
                     /gene="plsX"
                     /locus_tag="IW797_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ1224149.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(16343..18379)
                     /gene="recG"
                     /locus_tag="IW797_001366"
     CDS             complement(16343..18379)
                     /gene="recG"
                     /locus_tag="IW797_001366"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ1224150.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(18496..20178)
                     /locus_tag="IW797_001367"
     CDS             complement(18496..20178)
                     /locus_tag="IW797_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ1224151.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(20230..20592)
                     /locus_tag="IW797_001368"
     CDS             complement(20230..20592)
                     /locus_tag="IW797_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1224152.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            20862..21050
                     /locus_tag="IW797_001369"
     CDS             20862..21050
                     /locus_tag="IW797_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ1224153.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            21288..23957
                     /locus_tag="IW797_001370"
     CDS             21288..23957
                     /locus_tag="IW797_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1224154.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(24048..24668)
                     /locus_tag="IW797_001371"
     CDS             complement(24048..24668)
                     /locus_tag="IW797_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1224155.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            24780..25181
                     /locus_tag="IW797_001372"
     CDS             24780..25181
                     /locus_tag="IW797_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224156.1"
                     /translation="MKKLFSALFILLFGFIGIGTSENIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            25316..25654
                     /locus_tag="IW797_001373"
     CDS             25316..25654
                     /locus_tag="IW797_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAQ1224157.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         7013 a         5494 c         4637 g         8756 t
ORIGIN      
        1 gaaaaaacac ataaattttt acatgcaaaa aagcttttga tcagattgac atcaaatagg
       61 acaatctgtc aaaagctttt tcttttaaaa tacttcagga tataacaatt tcaatacatt
      121 catcgtcaat cttctgcctt ttcctgcaaa gggataaaca tgcatatgca tggctggatt
      181 gaaatttgct tcgatgatcc catatgcttt tttatcagtt gtaggatcga tttctttatc
      241 tggtataatc aaatcgatac cactaatctt agctcctagt gcttctaccg cagaaacggc
      301 gatttttttg taagcttcag aaaattcatc agtcatatcg atcgaatcac cacctgtact
      361 gatatttgag ttctccctta gataaacaat ttgattggct tggggaactg attcgatcgt
      421 caacccttgt tctttcaaca tcaattgttc taattcacct aattggatca attctaaagg
      481 tgcacgatga ttcgttccac gcaaagggtc actatttttt tcttcaacaa gttctttcac
      541 tgttcggata ccgtcaccaa taacattagc agggactcgc aacatgatgg cttggacttc
      601 tccgtcgatc acaaagaaac gatattccgt tcccggcata aattcttcta ctaaaaccga
      661 actgtcttca cgaaaagcaa tggctaaacc tgcttgataa tcttccagac tagctccttc
      721 cttgaaaatc gtgataccta acccgtaatt ggttgattta ggtttgatca caaatgcctg
      781 ctcggcaaat ctcggataag cttttaccgc ttcttccatg gatgaaaatt ctgcgcctcc
      841 tggtacccgg aacccagctt cttttaagac ttttttcgtt actgttttgt tttccatgat
      901 aagtgggacg atatagctgt ccttacttgt catgtttgca tttttcacat attctacatg
      961 gtcttggaat tgcagacgca aaaattgatc cgactcatct aatactttga cttggacacc
     1021 tttttggatg gcatcgaaca taaatatctg agtagaaagt tccatttcgc gaaaaccggc
     1081 caactgatag ggacgttcat ggctttcttt ataatattga tgtcccaatg ccacagcaac
     1141 ttcttgctga ctcgatagct gtgcttcttg atagagtttc gctgctaacg tttgttcagg
     1201 atgatccatt tgagtcaatg cttcctctac taaataaaga gaatccaacc aatccaattc
     1261 aaccagcatt tgcttcatcc cttcaagaat ttctctacct tcttgatgaa attctgtttg
     1321 atctgatggt tgttctagtg ctactttgtt atttcgagtc gtaccttctg ctacccattg
     1381 gtcacagtct tctttttctt ctgtccataa catgtacatc aggaataatt gtaagaaccg
     1441 aacttgctct ggattgatac ccaatcttgc ataaggattc aaatcgatat tccgcaattc
     1501 aatataacga acacctgttg ttgctaaatc tgcgactttc tttccgcctc ggaaacgaag
     1561 cggtgtataa aattctttct cctccgaaag tttcccttct tcgatcattc gctcgatatc
     1621 tgctaaatac tgtttcatag aagcatagga aactttcaca tttggatgat tcgtatagcc
     1681 cagcgcactg ttacgaatac tgcgaacagg ttcttgcgga tgtgattcat ctaaaaagta
     1741 gttcttctca ctcattggtg aagcaccgaa taaataagtg atcatccagc gatagcgcat
     1801 aaagtttctc gccgttttca aataaagttc tgttttgaat tcggaaaaat cttgaaattc
     1861 ttcctgatgg ctgaacaatg ttcttagcaa ctcatcgcca aattcaaaat tgaaatggat
     1921 accgcttacc atctgttttc gcttcccgta ttcttttgct aaataacgtc gatacaagac
     1981 atcttcgaaa ttttttaatt ttgcaataat gatcgcttcg tctttttccg gtaaggcagg
     2041 tggcatactt aatggccaga tcatctcttc tttcggtata gaacgagcag tcacatcata
     2101 aacagcagcg agatactgaa ataattcggg aatagaatcg gtaacaggag tgatcatctc
     2161 tgtttgtgtt tcgctaaaat ctgtttggat ataggggtga taggatcgat cgccaaagat
     2221 tgctggatga tcggtttttg ctagatttcc tgcaagatca acccgctgtc cttcccgttc
     2281 tataccaaat cgagcttggt cgataaaagg gcgcgcattg acatgcaata ataattgctt
     2341 aaaattcatc attaagtgac ctccaagtca tatgaatcta attataatca aaaagtctaa
     2401 gaaaaagaac gaaattgttt gagaaccgct tacaaataag tataaaaatg aaggcatcaa
     2461 aaacacagct atgttaaaaa caatcattca aacaaggagc tgcaactttt gtcacagctc
     2521 ctgcttttta gacatgggtt attcttcttt tagtaatcct ttgaacaatc caacgacaaa
     2581 atcatttgga tcaaacggct ccaagtcatc gattccttca ccaagaccga ccaatttcac
     2641 tggtaaatgc aattcattac ggatagcaag tacgataccg ccctttgctg taccatctaa
     2701 ttttgtcaaa acaagaccgg tcacatctgt tgtttctttg aattgttttg cttgaaccat
     2761 cgcgttttga ccagttgttg catctagtac aagaagtgtt tcttggggtg cttctggtaa
     2821 ttcccgctgg attactcgtt ttattttttc taattcgttc atcaagttca ctttattttg
     2881 cagtcggcct gcagtatcaa ctagtaaaac atctgcctgc tctatctttg ctctttccaa
     2941 agcatcgaat acgacagctg ctggatcgcc gcctgcattg ccacgaacaa cttcaacacc
     3001 agcgcgttcg ccccagacaa ctaactggtc gattgctcca gcgcggaagg tatctgctgc
     3061 tgccatcaat acttttttac cttcgttttt gaactgatgg gcaagtttac caatacttgt
     3121 tgttttccct acaccattaa caccgacaaa caaaataact gttagtccgt cttcttggat
     3181 atttagttca tttacttcat tgattccttc cgcttcatag agatccacaa gtttctcgat
     3241 gatcgcattt tggacttctg ccgatttttt cgcatttctg agtttcactt cttgacgtaa
     3301 tgcttcggtg attcgtatcg ctgtttcaaa acctacatct gcaccgataa gtgtttcttc
     3361 taattcttca aaaaagtctt catcgactgt tcgaaaattg gcaaacaact cattcatccg
     3421 ttcaccaaat gttttacgcg tcttttctaa acccttgtcg tacttctcct gtacttcaac
     3481 ttcttctgtt ttctcttcgc cagtaaaagc acgcttgatt ttatcaaaaa atcccatttg
     3541 actcccccct actctaatac aaatttttgg atcactttgg ctacgccatt ttcttcattg
     3601 ctggctgtca cgaaatctgc tgcttctttg acgaaaggca cagcattctc catcgctacg
     3661 cctagtccag cgtattggat catcggtaaa tcattttctt catcaccgat tgccatgatt
     3721 tcttcttgtt tcaaaccaag atcttttgcc aataaagaaa taccataagc ttttgtaaca
     3781 ccttttggca taaattccaa aagcatactt ctcgttttga tgatctcata catttcatgg
     3841 aattctgctg gtatttcttt gattttagga tctaattctt cttggtcata agcgattact
     3901 gccttattaa gcacaaattc atctgtaata tcgcttatat ccgctggttg gaattgcagc
     3961 agcttattca acacattgta caaggattgc ttccttgggg cactaggcag ctgtaaaacc
     4021 acattgtcag acaaaacatc tagtggcaga tccaattgct gagccagttt gtataagcgt
     4081 tggatatctg atacttccat cactttcttt tccatgattt caccagtatc atttttttgt
     4141 accaaaccgc cgttgaaagt gatgctgtag tcaccttcat ctaccagtcc taattcttgc
     4201 agataatgca ccatcgcagc taaggggcgt cctgtacata gaacgatctt gaccccttgc
     4261 tgttttgctt gtgctaatgc ttgtttattc tcatctgaaa tcagcttttg ttcatttaat
     4321 aatgtaccgt ccaaatcgat tgctactaat ttgatcattt tcgttcctac actttcgttt
     4381 cgttgatttt cgcctcttca ttgatatctt ccatccgtac agaaacgatt tttgatacac
     4441 cggattcctg catcgtcact ccgtacagta catctgctgc gaccatcgtt cctttgcggt
     4501 gtgtgactac gataaattgt gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt
     4561 tcacgttggc ttcatctaac gctgcttcta cctcatctaa gatacaaaac ggaacaggac
     4621 agacacggat aatagaaaag agcaacgcga tggctgtaag tgctcgttct cctccagaaa
     4681 gcaaacttaa actttgcagt tttttgccag gaggttgtac ttcaatctca atacctgttt
     4741 tcagcaaatc agttggttct gtcagtacta attcagcttt accgccgcca aacatatttg
     4801 gaaagacaat tttgaattct tgacggatag cttcaaatac ttctttgaat cttgtccgca
     4861 cttcatcatc catctcatcc atcgtttcaa acaattggtt tttcgctgct aacagatcat
     4921 ctcgctgtgt tgctaagaat gtatgtcttt cactgacttg ttcgtattgt tcgattgaat
     4981 ttaagtttac tggtcccagt ttttcaatct gttcttttaa ggatgaaacc tttgttctac
     5041 tgtcttcgat atcggttgtt tcttgatagt cttgggaagc tttctcaaat gtcagctgat
     5101 attctgactg cagataggtc aaatgattat ctaaaatcat ttcagctcgg tctttttgga
     5161 cttctaattt ggattgttcc gacagtcgtg ctttttgctc acgattacgc tctgccaaga
     5221 ctgcttcaag cttatcgatc tccttttgcc ttctatctcg ctgctctttg gcttttgcca
     5281 actcggcttt caatgtttct cgctgagcag ataattcgtt aatttgtttt tccaaacttt
     5341 cttctgtgac ttcatgatca gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg
     5401 cttcttgacg ttcggttgcc tctgcttttt ggactcgggc accgcggagc tggatctgca
     5461 gatgattaaa ctgctctttc aagacagcaa gatctgcttg ttcttgtgct ttttgacttt
     5521 gtacttgtgc tcttctggct tccatctggt cgctttcttg acttaacgat ttgatttctt
     5581 cgtcgatttt ttgtcgttgc gattcaagat ctgtttgttt gtctttcaat tcgttcgttt
     5641 gcttttcgta atcttcgata aactgctgca gttcacgtgt ctcaaaagtt gagatttgct
     5701 tttctttttc aaaacgttca agttcattta tgatgttttg cagctgattt gtcgcttctt
     5761 gttcttcaaa acgtaattgt tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac
     5821 gagctgtttc ttgttgcagc tcctgaactt ttttttcttg tgcttggagt tgtttatctg
     5881 cttcttcaaa ttcactagtc aactgtttca attcttgatt ttgtacaaac aaactgcctt
     5941 ggtttcctcg tttattcgct ccacctgtca tggatcctcc ggcattcatc acatcacctt
     6001 ctaaggaaac cactcgatat tgatagcgga tcgtttgcgc gattgcattg gcactagtta
     6061 agtctttggc taaaaggatc gttcctagca ggttttgaac cacagtttgg atttgatcgg
     6121 gataactgac ttgttcactg gcaatcccta taaacccttc tacagctgct gcttgtgtca
     6181 atatatgagc cggtaattgt cttggtttga ttgtcgtcag tggaaggaat gttgctcttc
     6241 ctcctctttg ctgtttcaaa tacgtgatgg cttgccgagc atctttttca ttttcaacga
     6301 tgacatgctg ggctgcccca cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa
     6361 catcgatcag ttcagccact gctccgacaa tacccgaaag ctgctgttta tgctgcaaga
     6421 ccaatcgaac cccttggtaa aaaccaaaat agttttcttg gatttcttga aggcttttct
     6481 tacgagcacg tagctgctgg acttgattca ttaattgata cattttaggc tgttcttttg
     6541 ctaatgcttc ttggaacttt ttcccgtttt gttggatcat ttctaacttc tcttttgttt
     6601 cagttaaaga tgattttagc tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca
     6661 atcggtctac tgatgcctct acttccgatt gcttggctaa cgtctgctta ctttttgctg
     6721 tttcttgtac atattgacgt tctagatact tcagttcgtt tcctacagct gcttgctctt
     6781 gcatcaaatc aacatattga tctcttagtt ctgccagtaa ttctttcgtt gatttttggt
     6841 atttttctaa ttcttgctgt gtttttatga gacttgcttc agctttttgg atttctgttt
     6901 ctttttctgc tacttctttc aacaagcttt cttgaagctt ttcaaaatgt ttaacctttt
     6961 tttgtgcttc tgctaatgaa gtttgatatt cttgcgagct tttttgtgta tgctttgtcc
     7021 gctcctgcaa aacatctttt tgtccttctg tctgcttcag tttttccgaa agatctaata
     7081 atacttgctg atttttttcg atcagacgat cggcttgcgc attttcttta cgctgcttcg
     7141 ccaaaatgga ttcttgttct tggatcgact cagatagttt gcctaattct agattgaatt
     7201 tcgcaagctg tgcttgtttg ttgtcccaat ctttttttgc tgtcttgatt tcggcaacca
     7261 tcaaagagac atcggtttga gtcagtgttt cttttagccg taaaaactcc tttgctgcct
     7321 cactctgggc ggccaaagga gttaactgtt cttccaattc gtggatgatg tcttggacgc
     7381 ggctgaggtt gtcttctgtt tcaaaaagct tctgttctgc tttctttttc cgctgcttgt
     7441 atttcaagac accagctgct tcttcaaaga tcccacgacg gtcttccggt ttactactga
     7501 aaatcgcttc tacttttcct tgagaaatga ttgagaacga ttcttttcct aatccagagt
     7561 ctaagaacaa ttcttggata tcttttaatc tgcagctttg tttattgatg aaaaactcgc
     7621 tttccccagt ccgtcgataa cggcgggtta cactgatctc attgtactcc aatggcagat
     7681 aatgatcact attgtctaaa atcactgtga cttctgcaat attcagctgc ttacgggtat
     7741 cggaaccggc aaagatgata tccggcattt ttccgcctcg cagactttta gcagattgtt
     7801 ctcctaatac ccagcggata gcttctgtaa tattgctttt tccactgcca tttggtccaa
     7861 ccactgctgt gacgctattt tcaaagtcaa tgactgtctt gtctgcaaat gatttaaaac
     7921 cggctatttc aattcgtttc aaatacaaag ctttccctca cttactttta cagtgctgcc
     7981 aatgcatttt ccgccgcagc ttgttctgct aattttttcg attttccttg tccagtgcca
     8041 atcaattgat catctacata aacttcgatc caaaatactc gttcatgagc tggaccttct
     8101 tcattgatca atcggtattc gattgataca tcacctgatt tttgcaagac ttcttgtagt
     8161 tttgttttat gatccatctc atgtgaaaaa gcacctgctt tgactttagg aaaaatcact
     8221 ttttcgatga atgcatgggt cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa
     8281 gcttcgaata ggtcgcaaag caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc
     8341 ttgcccaaca aaacatattg atcaaagtga cattctttgg caaatttcga caagctgtct
     8401 tcgcgaacga tcgcagctcg tgtttttgtc aattttcctt ctggcaaatg aggatataaa
     8461 cggaataaat actgagaaac catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc
     8521 tcattatccg ataattgcag attgcgatgc tcattcacat aggatgaatg ggtaaaagct
     8581 tgttcaagca aatttagatc gtgaaaaacg atgttatagt tttcttttag ttcttttgtc
     8641 agctggttgt ccattttcca cattcctttt atggatattt tttcgatact tccttattat
     8701 actggttttt tgtcaaattt gaaatggttc ggtcaaaagg taagtaaagc tttaataatc
     8761 ctagtgattg acgagaagtt gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt
     8821 tgtgaaaatg gctttccata tttttgcata aatttgactt aataccgaga aactggactt
     8881 tgaacaccgt ttattggaga agtcatgaaa tcagcgcctt gacctgaata ttagagaaat
     8941 aaacagaaaa atatcaaaaa accgaaaaag gacatcgccc tttttcggtc atttttagtg
     9001 attatgaacg tgaatctgct gtaacagaaa catttgccca tgggtttggt acatcataag
     9061 accaatccca gtctttgaca cggtcgttga ctggcaatac ttcattacgg aacaacgttg
     9121 gaatcgcaaa tgcttcttca aatgcatatt gttgccattt ttgatatgct tctttttggt
     9181 actcttcatc aaatgctttg cttgaactga tatcgttgat caattttgta ttttcttctg
     9241 attcataacg agtgtagttg aacgcagcat ttggtcccca tagtccactt ggatttggat
     9301 cagttcctgt attccaagca gcttggtaaa catcaatttc aggatcatca ttttctaatt
     9361 tatcatagaa agaattgaat tcgatcagac gacctgttgt gtattggaca tccaaaccga
     9421 tttctttcca ctgttggata tagtaatctg ccaatggttg cgctgtttcc ccaccggaca
     9481 tcgaagcaaa gttgattgtt agtttatttc catctttgtc ttctcttaaa ccatcgtcat
     9541 ccacatcttt gtagccagca tcatctaata gttttttcgc tttgtctaga tcttgtgtga
     9601 atcctttcat ctcatcatca tggtatgact ggaatactgg aggaatcaat gaagtcgcat
     9661 tgcttctcaa acctgcatag aaacgctcac ctactgcatt attatctaat gcatagccca
     9721 ttgcttgacg taatgattta tcagccattt ttgaatctgg attgtattcc acactctttg
     9781 tatctgcatt ccatttacct aacttgaatc ctagataagt ataagaagtt tgttcacggc
     9841 ctaagtttgt atagccagca atttctttat aggtatcgta agtatctgtt ggcatttgga
     9901 aaatcatatc atataattta gagttcaatg cttcagttgc acttgccatc ggtacagatt
     9961 tcatcgtgat ctttttcaat ttaggcgtac cattccagta gtgctcgttt ggcaagtatt
    10021 ctactgattc tcctgcaacg atattactca tgtaataagg gccaaaagtc actggatttt
    10081 tacgtacttg atcgcttgat tccatatctt tgattgctac accttctaac gtatgttttg
    10141 gtgctgcata tgaccaaata ccaccacccg attgaagcat gcttgggttt acttctttat
    10201 attcgatttc aactgttttg tcgtctattt ttttgattcc ggagattgtg tcggcacttc
    10261 catcatgata ttcttccata cctacgatgt tacggaaagt gtcgtcataa cggataccag
    10321 tgtaatcttt attcccaatg atttcgtaag ggaaaatcac atcgtccgcc gttacatctt
    10381 caccatctga ccatttgacg ttgtctttta ctttgattgt cgcttttttc gtatcttcat
    10441 ctagatcaag cgttgcagct ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata
    10501 gcggttcatg agaaggagcc ataaaagctg catcatatga atcttggtaa aactcccact
    10561 ggaacaatcc tttaaactga ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga
    10621 ttgcatcatc attatttttt gttgcaattg gaaaagttaa ttcctctttg ttattgccgt
    10681 tgcctccaga gttgtttcca gaggtatctg tacctccgcc cccgcatgct gcaagaacga
    10741 cagcagacat cagcgctgca aatccaaaaa tcgttttctt cattcctatt tcccccttcg
    10801 cttctatact agcctaaccg ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca
    10861 taattgatac tcaacatcaa aactaaaata aggattgatg cgggtaacca gatccattgc
    10921 cgattcacta agacttctcc gttacttgca taaccgatca atgtacctaa actaggtaca
    10981 tttgccggca agccgaaacc taagaaagtc aaagttgtct cgataccgat gtttgccgca
    11041 aagttcatcg tcaaattagt aatgattaat gaacttagat tcggcatgat ttcgcggaac
    11101 atgattttaa aatcattggt tcccattgtt tttgaggcac tgacataatc tcttcttgct
    11161 tcagataatg tcttgcttcg gaataatcgg gctttagcta cccaataaaa tgcactcatg
    11221 atgaatataa aggaccagac attgtatctg gaaataatcg atacgaagac gataatgatc
    11281 agcatgatcg gtagaatcat gataaaatca acgatccgca tcaagatgtt gtcaatgatc
    11341 cctccgtagt agccagagat gatccctaat ccaaccccga tgatcgaagt aaggatcgtg
    11401 atcgcaaatc cgatcgtaac agagtttcta gcacctatga tcaattgtcc taatacatca
    11461 cgtccgcctt catctgctcc cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt
    11521 gcagcgtatt tgtcgaagat actaacatac atcactttgt cttgatcggt cagcatcgca
    11581 ccgataaaga tcactaataa taaaaccact agcaaaccta atgaaaaaat agcaatttta
    11641 tcttttttaa attctcgagc aatcattcgt atccccatag gaggaatact ttcttgaact
    11701 gctgtattgt ttttttcact catcgatcca ttctcctttc tgattggttt tattgaatcc
    11761 gaatacgcgg gtcaacgata ctcatgatga tatctgaaag taatgtcccc actagcgtcg
    11821 cgattcctaa aatcaatact aaggatgtga tcacggtata gtcccgttgg ccgatggaat
    11881 caataaatag ttttccgact cccggataac caaaaatctt ttcgataact acagaaccag
    11941 caatcaacga agtgatttca taccccattt gagaagccat cggcaacgct gcatttcgga
    12001 aaatatgtcg actgaatact ttattcgtcg gtaccccttt ggaacgggct gttctgacaa
    12061 aatcgagcga tttcgaatcg attacttcac ttcgcagata ttggattgtg acggcagtcg
    12121 ccaaaaatgc ttgggtaatt gatggtaaca gaatatattt caaacggtct atccattgag
    12181 tgataaatcc accttgcaaa cctagtgttt gtgatccact tgttgggaac cagcctaaac
    12241 gataaccaaa aacaaatagc atgatcaagc cgaagataaa taaaggaaca gcaaaactaa
    12301 agaagttgta gattacgaca atcttatcca agattgaatt ttgataccgg ccagcaagca
    12361 tccccagagg cacagcaatc aaataagtaa ggacaactgt caagatcgat agatataaag
    12421 tgttgatgat ccgttcgccg atcaagtcag caacagagcg tttgtataag aaactttgac
    12481 caaaatcgcc ttgcaaagca tttccgatcc aacggaagta ctggacatac catgggtcat
    12541 tcaagccagc tgcttgtcgc atctgttcga tcactgccgg atcctgattt ggattgatca
    12601 aaccggtaaa tggatccccc ggcatcgctt gggctaataa gaagatcaga acacttaaaa
    12661 taataacttg cgggatcatc aataaaacac gtcgcagaat cgttttccac attagcttgc
    12721 acctccatct ttcaacgcca ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg
    12781 accagtcgca tcatagtatt ctttttggtt ttggatatac tcttgttcta ctcttcgtcg
    12841 attttctttg tggctttccc tattttctac atcgatttgc ggaatagctg aaagcagacg
    12901 tttcgtatag atatgtcgcg ggtcattata aatgtcttct cttgtaccga tttctacaaa
    12961 tcgacctttg tacataattg cgatattgtc gcacatgtgt tttactacac ctaaatcatg
    13021 tgaaatgaat aaatagctga ggccgtattc ttgctggatt cgtttcatga aattcaatac
    13081 ttgcgcttga acagatagat ccagagcaga tacaggttca tcggctacga tcagttttgg
    13141 attggtagca acagcacggg ctactcctag acgttgacgt tgtccgcctg aaaattcatg
    13201 aggatattta tatagcgcat cttccggcat cccaacgata tctagcagtc cttttacctt
    13261 tttcttttct tcttgatcgc ttaaccgttc aaaatttcgg atgggttcag caatgatatc
    13321 caacactcgc tttttaggat tcaaactgga cattgaatct tggaagatca tctgcacatc
    13381 cttgttataa cccatttttt tgcggttgct tcgcttcgtt acatcttttc cttcatacat
    13441 gatttgtccg gctgtcacct tttctaatcc cacaacggct tttcccgtag tagattttcc
    13501 agagccagat tctcccacta aaccatatgt ctttcctttt tcgataataa aatccacacc
    13561 atctacagca tatacatgat ccgttactcg attgaagaat ccactgcgaa tagggtaatg
    13621 aactttcagg tctttgattg tgattaattc tgtcatctac acctctcctt tttcgtctct
    13681 aaagtggaaa tgcttatagc aggagcaacg taccaagtgg ttcggtgcga cttcatgaag
    13741 gataggctct tcttcatgat cgcttgctgg tatccatgga atacgtggtg caaaccgaca
    13801 acctttacga ggcatgtttt tcagagaagg aacgacacct tcgatcacat ggagttctgc
    13861 atcatgattt ccttcttgtg gtatagagtt cagtaaagag cgtgtataag gatgcttagg
    13921 atgatcaaac agttctttta cacctgctac ttcaacgaac tgtccgccat acatcactgc
    13981 cactcgatca gccatttctg ctacgactcc taagtcatga gtgatcaaga tgatccctgt
    14041 ttttgtttct tcctgaaggt ctttcagtaa atcaagaatc tgtgcttgga tggttacatc
    14101 tagggctgtc gtcggttcat ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc
    14161 gatgatgaca cgttgtctca taccgcctga tagttcatgg ggatattggc gtccaactct
    14221 cgcaggatta ggaatcccta cttgttctag caattctagt gcacgagctt gacgctgttc
    14281 tgcattcatc tttgtgtgat aggaaagtcc ttctttgatc tggtcttcga ttctcatgag
    14341 aggatttaat gcagacaacg gatcttggaa aatcattccg atatcatttc cgcggatttt
    14401 attatagagc gtttcgttca ggtcgattaa attcagatcg ttatataaaa tctcacctgt
    14461 aatttttgtg ttattaggat caagtaatcc cataatcgta gtagccaagg tacttttccc
    14521 gcacccagat tccccaacga ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc
    14581 atctactgca tcgtaatagt catctttgag tcggaatcct gtatgtaggt tctgaacttc
    14641 taataattgg ttatcggaca atcgaatacc cctctctgct tcctaaatgg aagtttttct
    14701 taataaaaaa ggacattaca cagaataaag cgttttataa caaaggttat tcttatcttt
    14761 gtgtaatgaa ttattaataa ttatatagaa aatcgcttta ctttgcaaca taatttctgt
    14821 ttatcgccag aattatcaga aaaatgactt ttacgtgtca aaaagattaa aaaaagaagg
    14881 ataaaaagct gtatgcagct ttttatcctt ctttgctttt atttgaaatt atttaagatg
    14941 gctgctgata taatctactg cgccaccgac tgtttcaatt tgttcagcgt cttcatctga
    15001 aatttccgta ccgaattcat cttcaagctc taaaacgaat tccataatac taattgaatc
    15061 agcttttaaa tcatctttga tgttcaattc atccgtcact ttatcggtat ctatctcaaa
    15121 atgatttgaa ataattttcg ctactttgtt aaatacttct tcgcgagtca actttttcac
    15181 ctccaagaat catacacttg tcaattacct acaccatggc taattttact ggcatttttc
    15241 aggaatgtcc accttttttt caatggaacg gattgcttca gatatccaaa tactattctt
    15301 cgcttttttc aaattgttcg actaattgtc caactacatc tgtctcaagc attgtgtgga
    15361 tttggcggat cgtatagcga acagcatcgg ggcctgttgc tccatgagtt ttaatgactg
    15421 gtgctttcaa gccaaacaaa acggcaccgc catgtttgga ataatccatt tctgctttca
    15481 aagaacgtaa gccgtctttt aaaagcatag cgcccatttt tcctttgatc ccttcattta
    15541 gaatagcaga ttttaataga ttcatcatat tcattgctgt tccttcaata gatttcaaaa
    15601 cagcgttgcc tgtaaatcca tctgtgacca ctacatcagc cacaccattg agtagctcac
    15661 gagcttctac gtttccaata aaattcaagc ttgtttcatt ctgaaggagt tcataggctt
    15721 ttttcgtcac ttcactacct tttgttgctt ctgttccgtt gtttaagagt gcgacacgag
    15781 gacgtccgat acctcggact ttacgtgcat aaaaagaacc taaaataccg tattgcaaca
    15841 aatgttctgg tttgttttct gcgttagcgc caagatccag catatcgaaa ccggcacctt
    15901 ctttccctac tactggcaag gtagacatca agccagggcg ttcgatattt ttgatacgtc
    15961 ctacgatgaa caaccctgct gctagtagcg cgcctgtatt ccctgctgaa aatacagcat
    16021 ctgcttcacc gtttttcact gcttgtgcag caaggaccat tgatgcagtc ttttttcgtc
    16081 gaattgcttt tactggctca tcatcactat tgattttttc gtctgtatgg ataatcgtga
    16141 tgtttttttc atttgttaag tatttcttga tttctgcttc ttttccgtaa agtaagaatt
    16201 ctacttctgg aaaatcttgc tgcgctaaag ccacgccttc tacaattgct tgaggtgcgt
    16261 gatctccgcc cattgcatct acggcaattt tcattgtttc cactcctttt tccgtcatca
    16321 gtataaagta tagcacagtc tgctaatcga aataacgatt atcctgattt tctatttgga
    16381 ggctttgcgc taaaggctga tactcaggaa gcatctgcca atcctttatc tgccaaaggg
    16441 ctgcagcctc ttctctggct acctccaaaa catttccatc cgtcaccaag tcagcaatcg
    16501 caaattgagg cagaccagat tgacggaagc caaatacttc accaggacca cgtaattcca
    16561 gatctttttc gcttagtaca aaaccattgg tggtctctgt catgattttc atccgttcca
    16621 cacctagttc atttttagga ttagcaacta agatacagta ggaagcatca cttccgcgtc
    16681 caacacgtcc acgaagttga tgcagctgtg ccaatccaaa tcgatccgca tccatgatca
    16741 gcatgacggt cgcgttcggc acgtttaccc ctacttcaat tacagtagtc gaaactaata
    16801 tctgcatctg gttgtctttg aaggcttcca tgattacttc tttttcctga ttcttcattt
    16861 taccatgtaa caagccaact tcatattttg gtgcaaatag ttcacgaagt ttttcataga
    16921 tttccactgc attttttaca tctaatgctt ctgattcttc aatcagcgga cagatgacat
    16981 acatttgatg tcctcttgcc agttcttttt tcgtccaatc cagtacactg tccaattgag
    17041 gtgttcttac ccagcgtgtt tcgatcggga tacgtccagc tggaagttca tcaatgatag
    17101 atacatccat ctcaccatat gctgtaatag ctaatgttct aggaatcgga gtcgctgtca
    17161 taaataagac atctggtcgc cagccttttt cacgaagtac tttccgctgg ttcacgccaa
    17221 aacggtgctg ctcatccgta ataactagac ctaaattctg aaattcgact ccatcttgga
    17281 tcaatgcatg tgtgccgatc acgatgttaa tttccccttg ctctagtttt tcaaggagct
    17341 cacgacgttc ttttgctttc gttgaccccg taagcaatgc gatggttact tcattgggat
    17401 caaacagctg ttgcaagctt tccatatgtt gttgtgccaa gatttctgta ggcaccatca
    17461 acgcaccttg gaaacctgct gtaacggtag cgtataacgc aatcgcggca accactgttt
    17521 ttccgctacc tacatctccc tgcaaaagtc gctgcatatg ctgcggactg cgaagatccc
    17581 gacagatttc attcgttaca cgtttttgtg catttgtcag ttcaaaaggt agcttttgag
    17641 taaattcttt caatttttgg acatcataaa ggatctccaa accattcgtt tctgcttttt
    17701 ctgctttttt caatccttga agacgcattt gaaataaaaa gaactcttca aaaacgacac
    17761 gtcgtttggc ttgatggttt tcttcgtggt cttttggaaa atgcatactt cttacagcag
    17821 aagctcggtc aagcagtcga tatttctcca caaggttgtt tggcagattt tcttcaatca
    17881 atgtgccgta ttcttgaaac ccttttttga ttagatcaac taatgtcgtt tgacgaattg
    17941 atttgttgac gtgatatatc ggcgaaaagt catccattgt ttgagaacct aaaatcttca
    18001 tcccgttcaa tgctttacgc tttgcatccc atttaccgta tacggcgatt tcctccgata
    18061 aaatgacttt ttcttttaga taaggttgat taaaaaaaga aacgttgaac acatcatgtt
    18121 cttgcatcat acgaaattgc aatcggcttt ttttataacc aaatcgattc atcacaggag
    18181 gcgagactac gattcctttc aaagtaacct tttcttgatc ttggatctcg tttagatttc
    18241 tttcctgaat atcttcgtag cggaaaggat aataagtcag taagtcttca atcgtttgga
    18301 tgcccaagct ggcaagacta gctgctcttt tttctccgac tcctgccagc atagtgattg
    18361 gatcatctaa agaacgcata aatttttcct ccctccctta acaaaaaaag gacttggtca
    18421 gcgatcatac ctgatcacta accgaaggcc ttttatgaat gaatactgtg ttagaaagat
    18481 attctgacac agaaattatt cagcagaaaa tagataagga tagacaggct gatctccttg
    18541 gtggatctcg atttccaaat caggatgttt ttctgttagt ttctcagcaa attcatttgc
    18601 ttgttcaact gttccttcat cacctgccaa gatcgttacg atctctgtgt cctcattgat
    18661 catttgttct agtgtactta acgctgttgc tgtgatatct gggttagaga tcaggatttt
    18721 tccatcgatc atgcccaaaa agtctccttc tttgatctct acaccgtcaa ttgaggtatc
    18781 tctgatcgcg tgagtgactt gaccgctcac aacgctagac aacatttctg tcatcgtttc
    18841 tttgttttct tctaaagaag cttgatcatt gaatgaaagc atagctgtca atccttggga
    18901 aatcgttctt gaaggaacaa ctgctactgg gatatctgct acttctgctg cttgatcagc
    18961 tgccatgaag atgttcttat tgtttggcaa gacaatcact ttatctgcgt ttacttcttg
    19021 aatcgcttta acaatgtctt ccgtacttgg attcattgtt tgcccaccac tgataacata
    19081 attagcgcct aaactttcaa acagctcttg gacgccttta ccagcagcga tcgctacgat
    19141 accatacggg atacgtggtg tttctttttt cgcaaactcg ttcacttctt catcgtgttc
    19201 caaaatcgtc tcatgctgca agcgcatgtt gtcgactttg attttgacta atgaaccgaa
    19261 tttttgtcca taattcaaga cttctcctgg gttttctgta tgcacgtgga ctttgatgat
    19321 ctcgtcatca ttgaccacaa gtaaagaatc tcctatacca tctaaatagt tacggaattc
    19381 ttcgtaatcg aatgtactat cgactgtagg tccttctccg atttttacca tgatttctgt
    19441 acagtaacca aaatggatat cttctgtcgc catttgacct tggacactgc gatgatgttc
    19501 tgcattgacc atatcatcca tttctgctgg agaaggttca tatgtttcat ccgcttggaa
    19561 ttctccattc aatgcactta aaaagccctc ataaacaaac agcagtcctt gccctccact
    19621 gtctacgaca ccgacttctt ttaggactgg aagcaagtct ggagttttag ctaaagcacg
    19681 ctttgcccct tttacaacag ctgtcattac ttcaacgaca tcatcagtac tttttgcttt
    19741 cttttctcca gctttcgctg cttctctagc tactgtcaaa atcgttcctt ctactggttt
    19801 cattactgct ttatacgcag tttcaactcc atgagtgaag gctgcagcaa gatcggaagc
    19861 atttaatgag acaacatctg gaatttgctt agaaaatcca cgaaacaatt gagacaaaat
    19921 aacgccggaa tttccgcgcg cacccatcag caatcctttt gaaagcattg ctgctaattc
    19981 tcctactttt tctgaggttg aatctactac tgccttagct ccactagtca tagatagatt
    20041 catatttgtt cccgtatcgc catctggcac agggaataca tttaatgaat tgacgtattc
    20101 tgcatttact tgcagacgat tcgcacctgc ctggaccatt tcctggaact ggcttgcact
    20161 tatttctgtt acactcacct agataatcct ccttcagatc gactgctgag ttaccagcca
    20221 gtctcctgcc tagtctggca gtacacgaac accttgtaca aagacattaa ctgaattggc
    20281 agttacgcct aataatgcct ctagattgta tttcactttt tcttggacgt tgcgggaaac
    20341 ttcagagatt ttcgtaccat agctgataat cgtataaaca tctacagcaa taccattatc
    20401 ttcttggcgg acaactactc ctcttgagta attttcttta cgcaaaatct cagtaatatt
    20461 atctttaatt tgatttttgc tggccattcc tacaacaccg aacacatctg tcgcggcgcc
    20521 accaacaact gttgcaatta catcattggt aatttcaatg gttccagctg acgtttgaat
    20581 ttttacagcc atgaatgagc ctccttaacg ggtaaaaaac tcccattttt cttctatcat
    20641 attttatcat agcgcaactt gaaataaaag gcagttaccc aaagtactca aaaaccttgc
    20701 aaaatttcag gtataacctt gtttaattta caattattct tctcaattgt caattgtatc
    20761 tatgaaaatg tacttgcaaa tttccattct atatgatatg ttagtctggt atgagctgaa
    20821 ttattctatt gctccaaatc aaggcaaagg aggaaattac catggcaaaa gtttgttact
    20881 ttacaggtcg caagacaaaa agtggaaaca accgttcaca cgctatgaac tcaacaaaac
    20941 gtactgttaa acctaactta caaaaagttc gcgttatggt cgacggaaaa cctaaaaaag
    21001 tttgggtgtc aactcgtgct ttgaaatcag gaaaagttga acgcgtttaa ttgaatgata
    21061 cttggaccgt tcagatagtg aacggttttt tatttaataa aaatttttaa gggtgggact
    21121 tttgattcct accctttttt tgcgtttatt tttgttatac gaacaaatta tgaagttatt
    21181 tattctaaat tttatttttt tgtgaaaatt agactctttt taagcagaga aaaacaagga
    21241 tatgataaac tgaatacaac ttcatagaaa tttggtggaa aataaatatg aaaaaaaatt
    21301 taaagtcatt ctttagagaa aacggacgct acgcagcagc tagtttcctg attccgtttt
    21361 tgattttagc acttgtttat ctaagtatcg ggatctatcc tggaagcagc agaagtattt
    21421 tggcaagtga tgccttttca cagttttcta attttcatgc cagcttccga aatgttcttc
    21481 ttggaaagca aagtattttt tatacatgga atgcttctct aggattgaat tatttatctt
    21541 taatttctta ctatctaggc gggatcttca caccactggt gttctttttt ccaaaccaga
    21601 tgatgccaga tgcactgtat gttctgactt tgcttaaggt cggctgtgcc ggattggctt
    21661 tttggtttct tgcacgtacg ttcaagattc ctcgctgggg gcatgttgca cttagcgtca
    21721 gttacgcatt gatctcattt atcactgcac attcggagat catcatgtgg ctggatgcat
    21781 ttgtttatct tccattagtg attttgggga ttcatcgtgt tatggatctt aaacggccga
    21841 aacttttatt tatcagttat ttactcttgt ttttatccag tttttatatg ggttttatga
    21901 ttggcgtatt ttctttctta tattttatcg cccgtctatt gacaaactgg tcagaatata
    21961 aaaaaacgat tgttccttat ggtatcactt ctttactggc aggcggcgct tcaatgatca
    22021 ttgtattgcc agcagtgctg gatcttcggt caaatggcga ggcactgagt cagatcacta
    22081 cgttgaaaac agaagcgact tcttatctgg atctaatcat gaaaaatatg attggtgttt
    22141 atgacacgac aaaatacgga tcgattccgt tcgtttacgc aggtctgcta ccattagtcc
    22201 tctgtctgtt ttattttgcc agtcggaaag taccgttgaa aaataaattt ttgtacggca
    22261 gtcttttcgc catacttatc gcaagtttct atcttgttcc attgaatttg ttctggcatg
    22321 gtatgcatgc accgaatatg tttttattcc gttatgccta tctgttctct ttcttagtgc
    22381 tcctgcttgc aggaaaagga tgggaaaaga tagaacaaga cgatcgtggg ctgttgttag
    22441 gaatcatttt ggtcgtagct gctctctttg cggtggcatg gggcatcaaa cctgaaggaa
    22501 gctacaccta tgtgacgatg acttcctttg tattgacagt ggttttctta gggatttatg
    22561 cttttgctgt gggattttat cagcataaaa aactttcatt caatcatctg agtatattgt
    22621 tattattgat catgtctgct gaagcactgg tcaatacgaa cagtatgatc catggcattc
    22681 ttgatgattg gaattatgct tcccgcagtt tatatactga tccttatcct gacttgaaag
    22741 ctcttgtaaa tgaaacaaaa aaagattccg actcgtttta ccgcttggaa aatctggatc
    22801 cagtatctgc aaacgattcg atcaactatg gttacagtgg tatcagtttg ttttcttcta
    22861 taagaaatcg taattcttcc tcttacctgg ataaattagg tttccgttca agagggacaa
    22921 atttgaatat ccgttataac aataatactt tgctaatgga tgggtttaca ggaatcaaat
    22981 ataacatttc taaaaacgaa agcacgttca gtaaatatgg cttccgccaa gctggtcaaa
    23041 gtggcgacta tacactgtat cagaatgtca atgcattgcc actagcattt actgcaccgt
    23101 tgtccgttaa tgatgttgag cagccagtga cagataattt gaccagtcag acaaacttga
    23161 tcaatacatt gtctggttta aatgaacagt attttacatt ctatacacct actttgaaga
    23221 atcagttgaa tacgacgatc acaaagacaa cgactggcgt cacttactca gaaacagcag
    23281 ataaccagcc aaaagagctc acttgggaag tccatgtgcc tgcaaataca caagcttact
    23341 taagtttgtt cccaaccaat tttgcacaat tagaaagttc tagtgcgacg atcactgtca
    23401 atggtacatc gcgaaaaacg caaatcaaca tttctggtca atactatgat cttggctatt
    23461 atcctcaaga gacaacagtg acatttactg caagttttta tggaacgaaa gaagtaagtt
    23521 tcatggagcc taaagtcgtt ggtctagatg ttgtagccta tcaagccgca atgaatcaga
    23581 tcaaagaaaa aggtgtcgaa atggaagcca ctggcagaaa agcttctggg actgtaaata
    23641 ccgctgaaga ttccatgttg gtgacaacta ttccttatga caaaggatgg aaagctaaaa
    23701 ttgatgggaa accagtcaag gtcagcaatt tcaaagacgc attcttgatg ataaaagttc
    23761 cagcaggtaa acatactgtc actttcagtt acttgcctga aggatttacg ataggtgcaa
    23821 tcctttttgt cctatgtatt gcattgttta ttctttatgt ctggtacctt gaccgcaatc
    23881 gcccctttta tggcacgatt gatccagcaa tagaagaaaa accgcgaaat acacacagaa
    23941 gaagacgaag aaaataaaga aacgatggaa tctgtgtcac tcttctattt aaatggcaaa
    24001 aatttcaatc tttaaatgac aaaaaccacc tgatttttgg tggtttttta tatacctatg
    24061 actaaactat ctcttgctgc ttcgacaact tctacagtga tcttatccat ttgattgatt
    24121 cttaaaatcc gatcaatctg cgcaaataaa cgatgaataa gcctaaaatt tccttttgtg
    24181 atcttaatga tagttgtgat agcctcataa tcagaaaaat cttctagctt gatatcaatt
    24241 cctaaatctg cccatttgta ttccaaaata tgatgtgttt cgtccttact aaggttatca
    24301 aattcatgtg caaatcctat gcgagagtaa agctgcggat aacgagacaa tcttttttca
    24361 ataccaggca taccgataag aaccattgcc agatcatttc tatcgtaaat atctcttagc
    24421 tgttctaaat gctgaagctt taaacgatca atttcatcga caataattaa gtcaattgct
    24481 tcataggttt taggcacagg aggatcttct tgattgtatt ttagtaccat atgcattgct
    24541 ttagtcagac ctattctact agttattgtg ttaatatccg aactgacacg agacatcttt
    24601 tcagctggta cagtataaaa aactttattt acacttaata tttcatctgt tgcatttatc
    24661 aagaacaaga aaaagtctga atattcaaaa aataacggtg tacattttta aaaggagatg
    24721 tataatcgag atttagaaag cgttttccta aaaatttaat gaattaggaa gtgattacta
    24781 tgaaaaaatt atttagcgca ctttttattc tgttatttgg ctttattggt attgggactt
    24841 ctgaaaatat agttaaagct agcacgatgg ttgaacaaac aagtgaaatg gcaaaaactt
    24901 ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc caaaggattt agtgataaag
    24961 atacttttta cactcaaaag atagagcctc aagatccgaa aacacgtttt tctgcaaatg
    25021 tattgcggtt aaccgcatct actaaaaaaa ttactaatac gcaggggcgg acagaatata
    25081 ttataacaag tcccaattcg gcgatgtatc gtatttctac acgcttgagt ttaggtagtg
    25141 caaaatcata tactcaaaca gtaaatctag ctaattctta aggttatggt ggaggaatgt
    25201 attttacatt tactgggaaa agacaatatt acagtgtaaa attagctatg caagtttata
    25261 atacatttgg tgtagggtca gtctcttgca ctgcgggagg attgacaata ggaaaatgat
    25321 aatttcttac tatgtcttaa atagaagtga tgatgaagga ctcttcgttt ttcaatcatt
    25381 acatgaggat acgatgctaa aagagtttta taatcggatt aagaataaac agatagaaac
    25441 aaatttatat tttttaagtg actctatctt gccttttatt agcaacccta aaattcttaa
    25501 cttgttgcag acaaaagatt tgaaaagttt accgattatt gtcattgatg aggaaattgt
    25561 aaaatatggc gcttatctaa gcgtagaaga attagaaaaa ttaactaaca ttggaatgag
    25621 tattcaggag gataatgggg tacatggaac atagaatgaa tatgcacagt tgagaacgga
    25681 atttgacatt tatttagacc aacagggtat accctgttgg tctttttgtg cgttcatgat
    25741 atacttggtg tcatttttat atttaaatgg cacaaatatt tctaattatt tgtcatttaa
    25801 cgctgaaaat ttttgctatt tatctaaaaa agtgacagga tactcatttg gccttcatag
    25861 aattttctaa atatgaatca tcaatgaatt taattaaaaa
//
LOCUS       DACEPD010000036        25536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.036, whole genome shotgun sequence.
ACCESSION   DACEPD010000036 DACEPD010000000
VERSION     DACEPD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.036"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW797_001374"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IW797_001374"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ1224158.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IW797_001375"
     CDS             complement(937..1518)
                     /locus_tag="IW797_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1224159.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW797_001376"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IW797_001376"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ1224160.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IW797_001377"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IW797_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ1224161.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IW797_001378"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IW797_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ1224162.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IW797_001379"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IW797_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ1224163.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IW797_001380"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IW797_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1224164.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW797_001381"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IW797_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ1224165.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IW797_001382"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IW797_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ1224166.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IW797_001383"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IW797_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ1224167.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IW797_001384"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IW797_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ1224168.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IW797_001385"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IW797_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ1224169.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IW797_001386"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IW797_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ1224170.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IW797_001387"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IW797_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ1224171.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IW797_001388"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IW797_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ1224172.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IW797_001389"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IW797_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ1224173.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IW797_001390"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IW797_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ1224174.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW797_001391"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IW797_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ1224175.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IW797_001392"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IW797_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ1224176.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IW797_001393"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IW797_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ1224177.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IW797_001394"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IW797_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ1224178.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IW797_001395"
     CDS             13972..14157
                     /locus_tag="IW797_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ1224179.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IW797_001396"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IW797_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ1224180.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IW797_001397"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IW797_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ1224181.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IW797_001398"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IW797_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ1224182.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IW797_001399"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IW797_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ1224183.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IW797_001400"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IW797_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ1224184.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IW797_001401"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IW797_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ1224185.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IW797_001402"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IW797_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ1224186.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IW797_001403"
     CDS             18232..18879
                     /locus_tag="IW797_001403"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ1224187.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IW797_001404"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IW797_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ1224188.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW797_001405"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IW797_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ1224189.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IW797_001406"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IW797_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ1224190.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IW797_001407"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IW797_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ1224191.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IW797_001408"
     CDS             20317..21255
                     /locus_tag="IW797_001408"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ1224192.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IW797_001409"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IW797_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ1224193.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IW797_001410"
     CDS             21935..22774
                     /locus_tag="IW797_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1224194.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IW797_001411"
     CDS             22750..23619
                     /locus_tag="IW797_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1224195.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IW797_001412"
     CDS             23609..24406
                     /locus_tag="IW797_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1224196.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IW797_001413"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IW797_001413"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1224197.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         8299 a         4712 c         5497 g         7028 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac cctcttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacatcctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tgtgacagaa gtggtcagct
    25381 tcaagaaata agaaggaatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag gagctacttc tggaacaccg tgaatcagag gttgtgaaaa agctgccctg
    25501 catcaagtaa taagaacaac caaacaaaaa tagctc
//
LOCUS       DACEPD010000037        25353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.037, whole genome shotgun sequence.
ACCESSION   DACEPD010000037 DACEPD010000000
VERSION     DACEPD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.037"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(127..504)
                     /locus_tag="IW797_001414"
     CDS             complement(127..504)
                     /locus_tag="IW797_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224198.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            760..1488
                     /locus_tag="IW797_001415"
     CDS             760..1488
                     /locus_tag="IW797_001415"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ1224199.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1488..1742
                     /gene="purS"
                     /locus_tag="IW797_001416"
     CDS             1488..1742
                     /gene="purS"
                     /locus_tag="IW797_001416"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ1224200.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1744..2415
                     /gene="purQ"
                     /locus_tag="IW797_001417"
     CDS             1744..2415
                     /gene="purQ"
                     /locus_tag="IW797_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ1224201.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2416..4638
                     /gene="purL"
                     /locus_tag="IW797_001418"
     CDS             2416..4638
                     /gene="purL"
                     /locus_tag="IW797_001418"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ1224202.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4623..6062
                     /locus_tag="IW797_001419"
     CDS             4623..6062
                     /locus_tag="IW797_001419"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ1224203.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6094..7137
                     /locus_tag="IW797_001420"
     CDS             6094..7137
                     /locus_tag="IW797_001420"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ1224204.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7134..7712
                     /gene="purN"
                     /locus_tag="IW797_001421"
     CDS             7134..7712
                     /gene="purN"
                     /locus_tag="IW797_001421"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ1224205.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7709..9244
                     /gene="purH"
                     /locus_tag="IW797_001422"
     CDS             7709..9244
                     /gene="purH"
                     /locus_tag="IW797_001422"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ1224206.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9260..10534
                     /gene="purD"
                     /locus_tag="IW797_001423"
     CDS             9260..10534
                     /gene="purD"
                     /locus_tag="IW797_001423"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ1224207.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10656..11570
                     /locus_tag="IW797_001424"
     CDS             10656..11570
                     /locus_tag="IW797_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ1224208.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11703..13268)
                     /locus_tag="IW797_001425"
     CDS             complement(11703..13268)
                     /locus_tag="IW797_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1224209.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13261..14712)
                     /locus_tag="IW797_001426"
     CDS             complement(13261..14712)
                     /locus_tag="IW797_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1224210.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14726..15742)
                     /locus_tag="IW797_001427"
     CDS             complement(14726..15742)
                     /locus_tag="IW797_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1224211.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15962..17050
                     /locus_tag="IW797_001428"
     CDS             15962..17050
                     /locus_tag="IW797_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1224212.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17104..18630
                     /locus_tag="IW797_001429"
     CDS             17104..18630
                     /locus_tag="IW797_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224213.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18632..19837
                     /locus_tag="IW797_001430"
     CDS             18632..19837
                     /locus_tag="IW797_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1224214.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20094..21071
                     /gene="guaC"
                     /locus_tag="IW797_001431"
     CDS             20094..21071
                     /gene="guaC"
                     /locus_tag="IW797_001431"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ1224215.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21335..22759
                     /locus_tag="IW797_001432"
     CDS             21335..22759
                     /locus_tag="IW797_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ1224216.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23220..24329
                     /gene="pdhA"
                     /locus_tag="IW797_001433"
     CDS             23220..24329
                     /gene="pdhA"
                     /locus_tag="IW797_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ1224217.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24332..25309
                     /locus_tag="IW797_001434"
     CDS             24332..25309
                     /locus_tag="IW797_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ1224218.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8198 a         4314 c         5696 g         7145 t
ORIGIN      
        1 aagaagcaga tccgttagcg gttctgcttc ttttagttta ttatattttc ttatcaataa
       61 taggaacctg attttaaaag cggctacctc tttaccgtgc ctctttacat acaaaaaata
      121 gaggaattaa gcgtaaatgg cttggctatt ataactaaaa tgctggttat ggagatgttg
      181 cagcaagttt tcgagaaaag cgtattccat ctcttgtcca ttgaataaga tctggatacg
      241 ataatgattt ttgaaacgct tctgttcatg aatctgactg attgaaagtt cgcctttgtg
      301 ttctgtctga taaagaaaag tccttttacg gcattcagca cagcgcttgg ttgctcggcg
      361 gatttttcgg aatgtataaa aactagtatc tttattgcat aaataaacgt ctttgatcga
      421 cagcaaatca ttatatagac aggaacgttc ttcagctgtt aattctgata aaaaaagctg
      481 accttgtttt attgcatctt ccatcggcaa gtgcctcctc gaacccatta tccaaatttt
      541 tagttaattc taatatacca tcattaagta taaatgtaaa cgcttgttga tggtataaga
      601 ttttcgtttc tgataaaaca cgaacattaa aagaatctaa tgccttaaat gtacatattt
      661 ttgttgattt ataaaaagat gattgctaaa atttaaagtg aaaaaacgaa caaaaaacga
      721 tgtatttgct tttaatgtaa ataaaaagga gagacgtaca tggagaagaa agaacttttg
      781 tatgaaggaa aagccaaaaa aatatatctt acttctgatg agaacttgat ttggatagaa
      841 tatttagatc aagcaactgc gttgaatggc gcgaggaaag accagataag tggaaaagga
      901 gaattgaata atcaaatcac aagtgcaatt ttccgttatc tgactcaaag aggaataaaa
      961 aatcatttta ttgaacaatt gtctaaaacc gaacaattag caaaaaaagt acaaattatc
     1021 cctctggaag tggttatccg aaatattgca gcaggaagtt tctcaaaacg tctaggtgtt
     1081 cccgaaggta aagaattcat caaaccaatc attgaattct actataagga tgaccagttg
     1141 gatgatccat ttatcaatga agagcatatc tatttcttac aacttgcaaa gccggaagaa
     1201 atcagtgagc tgaaagccca agcgttggct attaatgaaa ttttgaccga attatttgct
     1261 aaggtcggtt tggttttagt agatttcaag ctagaatttg gccggtcgaa tggacaaatt
     1321 ttgcttgcgg atgaagtaac accggatact tgccgattat gggatgaacg tacgaatgaa
     1381 catatggaca aagacgttta ccgcagagat ctaggtgatt tgatcccagt ctatcaagaa
     1441 gtatataacc gtctatcgga actaactgaa gaaaaggagc gagtatgatg tatttcgtaa
     1501 aagtatatgt aacctataaa gattcagtgt tagatcctca aggggaggca gtaaaaaaag
     1561 cagtccatcg tatgggatat gaaacaatcg aagatatccg aataggaaaa tactttgaga
     1621 tacaacttgc tgatgcagaa gaaccaatcg atacagtgat tgaaacgatt tgtgacaagc
     1681 tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat caaaaaaatg gaggaagtat
     1741 aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt gtgacgcaga tatgttatgg
     1801 gcagttcgcg agatcatggg cgctgatgcc gaatttgtac ggcatgatga gtcctcttta
     1861 gaaggatttg atggggtttt actgccggga ggattctcct atggtgacta tttgagatgc
     1921 ggtgcgattg ctcggttttc tacaatcatg gaagaagtga ttcgttttgc tgaagaaggg
     1981 aaaccagtat tcggtacatg caatggattc cagatattga cagaagcagg acttttgcca
     2041 ggtgtactga gacgaaatga gtctttgcat tttatttgca aaactgtacc attgaaagtc
     2101 aaccccaaga cggcctttac ttcagagtat gaagccgacg aaataatcta tcttccagtt
     2161 gctcatgggg aaggaaatta tttctgtgac gaagaaacac ttcaagaact aaaaaaaaat
     2221 aatcagattg cctttactta taatgacaac atcaacggca gcgtggaaaa cattgcaggg
     2281 attacgaaca aagcagggaa tgtgttagga atgatgcctc atccagaacg tgcagtagaa
     2341 gacttgctag gatcagaaga tgggaaacgg ttctttgctt ccattttaaa aaacttcgga
     2401 aaggtgaccg tatagatgaa aacattgaaa gaaccgacag ctgaagagat caagaaaaaa
     2461 agaatctatg ccgaatgggg attgactgat gaagaatatc gaatgatcga agaagatatc
     2521 ttaggaagaa tgcctaatta tacggaaact ggactgtttt ctgttatgtg gagcgaacat
     2581 tgttcctata aaaattctaa accggttcta cgcaaatttc ctactagtgg acctcatgtg
     2641 ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg gtgatggact agcagttgtc
     2701 ttcaaagcag aaagtcacaa tcatccttca gccgtggagc cttatgaagg agctgcgaca
     2761 ggagttggcg gaatcatcag agatattttc agcatggggg ctcgtccaat tgcgttgcta
     2821 gattcattga ggttcggcga attagataat ccaagaacga aatatttact agaagaagta
     2881 gttgcaggta tcggcggata cggcaattgt atcggcatac cgactgtagg cggcgaaatt
     2941 gcatttgatc cttgttatga aggaaaccca ttagtcaatg caatgtgcgt aggtttgatt
     3001 gaacacaaac atatccagaa agggcaagcc gctggcgtcg gtaattcgat catgtatgta
     3061 ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt ttgcctcaga agaatttaat
     3121 caagaagaag aacagcaacg ttctgctgta caagtaggcg atccttttat ggaaaagctg
     3181 ctgcttgaag cgtgtttgga attaatcttg catcattcgg attctctaat aggtatacag
     3241 gatatgggag cagcaggact tgtttcatct agtgcggaaa tggcttcaaa agctggctca
     3301 ggacttatat tgacattaga tgaagtccca caaagagaaa ctgggatgac accttatgaa
     3361 atgatgttat cggaatcaca agaacggatg ttgatttgtg ttaaatcagg agaggaagaa
     3421 aaaatccaag aactatttca gaagtatgat ctagatgcag tcactatcgg taaagtaaca
     3481 gacgacgggc aatatcggct atatcatcaa ggagaagaag tagcaaatct gccagtcgat
     3541 gcattagctg aagatgcacc agtttatcat aaagaaatga aagaaccagc tcgaatcgta
     3601 gaatttcagc agttagctcc ttatcagcca gttatcgaag aacctggaca agtattgctt
     3661 gatctgctgc agcaaccgac gattgcttca aaacgttcaa tctacgaaac ttatgattca
     3721 caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg ctgctgtgat gagggttaga
     3781 gggacgaata aagcgttagc tatgaccaca gactgcaacg cgcgctatct gtatttggat
     3841 ccaaaaatcg gaggacaaat agcagtggca gaagctgcta gaaatattat tgctagcggt
     3901 ggtaagccct tggcgatcac cgactgcctg aactatggtt ctcctgataa accagaagtt
     3961 ttttgggaat tatcgacttc agctgacgga attgctgctg cgtgtgaaac gttaggaact
     4021 cctgttattt caggaaatgt ctctttatat aatgaaacgg atggtcaagc aatctatcca
     4081 acaccaatga ttggtatggt cggattgatt gaggatcata aacatatcac tactcaggaa
     4141 ttcaaaaaat caggggatct aatctatata ttaggtaaga cgtttgcaga ttttgatggc
     4201 agtgaattac aaaagatgca gcttggaaga atcgaaggtg tgatccgaaa ctttgatcta
     4261 tccattgaaa agagaaacca agagttagta ttgacagcca tccaaaacgg attgattgaa
     4321 agtgctcatg actgttcaga aggtggcttg gcaattgcac tggcagaatc agctttcaaa
     4381 catcagctag ggatatctgt acagtttgaa ttgtcctctg ctcaattatt tgcagaaact
     4441 caatcaagat ttgtgttgac agtcgcaccg gaaaataaga cacgattcga agaaatgatg
     4501 ggagatgctg ctgttttagc aggtaaggtg acggatgaag cgataatcga aatttcagca
     4561 actgacggtc agatcaaaat agaaactgca gtagcaagaa agtgctggga ggatgcaatc
     4621 gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg gatcttcggt gtttggggtc
     4681 atccagacgc cgctagtgtg gcatacttcg ggctccatag tcttcagcat cgaggacaag
     4741 aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa ggggcatcga gatttggggc
     4801 tgctttctga ggtattcaaa gatcaaagaa aattgcagca gttaacagga gaagctgcaa
     4861 tcggacatgt acgttatgca acagcaggca atggaagtgt ggacaatatt cagccttttc
     4921 tattcaaatt tcatgatcag tcgatcggct tagcgcataa tggtaacttg atcaatgctc
     4981 gttcccttag aaaagagtta gaggaaaatg gggcgatctt ccattctaac tccgatacgg
     5041 agatattgat gcatttgatc cgacgaagtg aagaaacaga ttttttaaat aaattaaaaa
     5101 gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt aatgacagaa acggcaatga
     5161 ttgccgcttt ggacccaaat ggttttcgtc cattagcaat cggacaaatg aaaaacggag
     5221 catacgtgat tgcctcggaa acttgtgcat tagaagttat tggggcacgt tttataagag
     5281 atgtccagcc tggtgagata gtgatcatca atgacgaagg aatccagatt gattgcttta
     5341 cgaaggacac gcagctttct atttgttcga tggaatatat ctattttgcc agaccagatt
     5401 caaacatcgc agggatcaat gtgcatactg cacggaaaaa tatgggacgg aatttagcga
     5461 aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc taattcttct ttgtcagccg
     5521 caagcggtta tgcggaagct agtggaatcc cttatgaaat cggattagta aaaaatcaat
     5581 acattgcccg aacatttatc cagccaacgc ctgagctaag agagcaaggt gttcgaatga
     5641 aattatctgc tgtacgagga gtagtggaag gtaaaaaggt gattatggtc gatgattcaa
     5701 ttgttcgggg tacgacaagc agacggatcg tccatctttt gaaagaagca ggggcaaaag
     5761 aagtccatgt gaggattgct tctcctcctt tgaaatatcc atgtttttac gggattgata
     5821 ttcaaacgcg taaagaactc attgcggcaa atcacacgat tgatgaaatc aaagaatgta
     5881 tcggtgcaga ttctttaggt tttttaagtg aagagggtct gatcgaatcg atcggtctgg
     5941 aaaaagatgc tccgtattca ggattgtgta tggcttattt caacggagat tatccgacgc
     6001 cgttatatga ctatgaagaa aaatatcagg aatcactaaa agaaaaagta tcgttctttt
     6061 aaaagcagac caagaaggga gtatatgaat tcaatggcaa atgcatacgc aaaagctggc
     6121 gtagatgtcg aagctggata tgaagtagta gaacggatca agaagcatgt caaaaaaacg
     6181 gaacgtttag gtatgatggg cgctatagga ggattcggcg gttgttttga tttatcggtc
     6241 tttcaattaa aagagccagt ccttgtctcg gggaccgatg gcgtggggac gaaattagca
     6301 gtggcaatcg aagcagacaa acacgataca atcgggatcg actgcgtagc gatgtgtgtc
     6361 aatgatatct tagcacaagg cgcagagccg ttatatttct tagactactt agcaacgggg
     6421 aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg tagcagatgg atgtgttcaa
     6481 ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc ctggtatgta ctcaggtaat
     6541 gattatgatc tagccggatt tgctgtaggg atagctgaaa aatctcaatt attgacagga
     6601 gaaacgatca acccaggaga tgtgttgctt ggaatagcat ctagcgggat tcattcaaat
     6661 gggtactctc ttgttcgcaa aattttcttt gagcagcagc agttttcttt atcatcaaaa
     6721 ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg agccaacgaa aatctatgtt
     6781 tcttccttgc ttccgctgat caaagaaaag aaaataaaag gagctgcaca tatcactggt
     6841 ggagggtttg tagaaaatat cccgcgaatg ttaccagaat ctatggctgc acaaatcaaa
     6901 cttggctcat ggccgattct tccaatattc ggctgtttac aaaaatatgg tgaaattcct
     6961 gagatggaga tgtatgaaat cttcaatatg ggcatcggta tggtcctggc agtcgaagaa
     7021 tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg gcgaaaaagt ttatgaaatc
     7081 gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta taggagaaga acgatgagaa
     7141 tcgctgtttt tgcttctgga aacggaagca actttcaagc gctagccgat tatctatcga
     7201 aaaaaggatt ggaatcttcg attgactggt tgttttgtga tcagcctgaa gcatatgttt
     7261 tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt ttcacctaaa gaatttgatt
     7321 caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa agaaaaaaag atcgatttga
     7381 tcgtattagc tggatatatg cgaatcattg gaccggtatt gctgaaaaat tatgataaaa
     7441 gaatcatcaa tatccatccg tctcttttac ctgccttccc tggtttgcat ggcatacgtg
     7501 atgcctttga agctggagta aaagaaacgg gtgtcacaat tcattatatc gaccaaggtg
     7561 tagatactgg accaatcatc cggcaggaaa aagtgagaat tgaacaggag gacacgttcg
     7621 attctttaga agaaaaaatc catagagtag agcatcgaat ctatccagaa gtgattagtg
     7681 aaattattga aaatggaggc tatctgaaat gacaagagca ttgatcagtg tatcagataa
     7741 aactgggatt gtagcattcg cccaaggttt gattgaacaa ggagtagaaa tcatttcaac
     7801 aggaggaaca aagcatgcgt tggataaagc tgggatcaaa acgctttcga tcgaagaagt
     7861 gacagggttt cctgaaatga tggatggccg tgtcaaaaca cttcatccta aaatccatgg
     7921 tggtctttta ggaagacgcg atctccctag ccatatggag gcaatggaaa cggaaaatat
     7981 ccaaccaatc gactttgttt gtgtcaattt atatcctttc aaagaaacga ttatgaaacc
     8041 agatgtcgaa atagcagaag ctattgaaaa cattgatatc ggtggtccta gtatgctccg
     8101 tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt gatccatctg actatgaaga
     8161 agtactttca gaattacgtc agtatgggca tacttcactt tctttcagac aaaaactagc
     8221 ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg atcgctcaat acttaacgga
     8281 ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg tatgaacgaa aacaaacgct
     8341 tcgttacggt gaaaatagcc atcaagccgc tgccttttat cagtccgctc ttctggaaaa
     8401 atattcgatc gcagcagcaa aacaactcca tgggaaagaa ctttcttata ataatatccg
     8461 agatgcagat gctgcgctac ggatcatgaa ggaatatgac gagccaacgg tagtcgcctt
     8521 gaaacacatg aatccttgtg gtattgggac agcatctacg attttagaag cgtgggaagc
     8581 agcgtatgaa gcagatcctg tttcaatctt tggtgggatc atcgttttga atcgagaagt
     8641 agatctgcca acagcagaac agatgcataa gctgtttcta gaaatcatca ttgccccttc
     8701 ttatacacaa gaagctttgg aattactgca aaaaaagaaa aacatccgat tactgacagt
     8761 agactttgcg caggaagaaa atcaaaatcg agaagaaacg gtctcagtct taggtggact
     8821 tcttgtacaa gatcaagatc tggcaataga aaatgaagaa gagtggaaag tcgtgacaaa
     8881 acgtaagccg actccagcag aagcaaaagc attagtgttt gcttggagag ctgtcaaaca
     8941 tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag acagtaggaa tcggagccgg
     9001 gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa caagcgcaag agaaaatgga
     9061 aggtgcagtt ttagctagcg atgcttattt tccaatggat gattcggttg aatatgcagc
     9121 caaacatggt ataaaagcaa tcattcagcc aggaggaagc atcaaagacc aagattctat
     9181 tgatatggcg aataaatacg atctagcaat ggtctttact gatgttcggc attttagaca
     9241 ttagagagga gtcggataaa tgaacatttt ggtgatcggt gcaggtggtc gtgaacatgc
     9301 aataagtaaa aaacttttag ccagtccgaa ggtggatacg gtgtattgtg cgccaggaaa
     9361 tccggggatg acaaaagatg gtatcaattg tgtcgctatc agtgagcgag atcattcagc
     9421 gttaatccat tttgttaaga aagaggctat tgattggagt atcgttggtc ctgaagttcc
     9481 tttgttaaat gggatcgtcg atgattttat ggcggaagga ttgaaaatct tcggtccgac
     9541 aaaagcagca gctctgatcg aaggttcgaa aagttttgct aaacaattaa tggatactta
     9601 tcaaatcccg acagctaaat ccagaagttt tgaatgttac gaaaaagcag cagcgtatat
     9661 caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc ttagcagctg ggaaaggtgt
     9721 agtcgtggct accacagtga aggaagcgct ggatgcggca gaacaaatgt tgaaccagca
     9781 tcgatttggg gatagtagca aaaaaatcgt cgtagaagag tttttggcag gagaagaatt
     9841 ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg atggtcattt cgcaagatca
     9901 taagcgggca tttgataacg ataaagggcc aaatacaggc ggaatgggtg cttattcacc
     9961 agtaccgcaa atttcaaaaa agatgattga aaaagctgtg gaagaagttt taaaaccagc
    10021 ggctttggga atgaaagaac taggacgctc tttcacgggt gttttatatg cgggtttgat
    10081 tgctacgaag gaaggaccaa aagtgatcga attcaatgcg cgtttcggtg atcctgaaac
    10141 gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa gtaatcgatg atttattaga
    10201 gaatcgtaca gtagagttaa agtggcagca agaaggatac aatgtaggcg ttgtggttgc
    10261 agctgccggt taccccgaag aatatgagac aggaatggtt ctaccagatt ttctggaaga
    10321 agagctttct gtttactatg ctggagtttc ctcaaataac ggcaaactag ttggagcagg
    10381 aggacgtctt tatttagtag aagcatacgg tgagacgatc aaagaagcac agaaaaaaat
    10441 ttacaccgcg ttaatggata aaacgacaga tggaacattt tatcgtacag atattggaga
    10501 aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc tgcttccatt tataaaaaat
    10561 acaacaaaca taatcggaag cgccgatttt agtgaagcct tttcataaag agtgtggtac
    10621 acttttgttg tgtatataaa taaggaggag ttcagatgaa aaaaattcaa ttaggtactt
    10681 ctgatcagaa agcagctgca gtgatcttag gctgtatgcg tatcaacaat gcggaagatc
    10741 cagtaaaagt gattgagaca gcttatgaaa acggcattga tttttttgac catgcagata
    10801 tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc tctagcaaaa acatcaatca
    10861 aacgagaaga tctttttatt caaacaaaat gtggaatcgt tccaggtgtg atgtttgatt
    10921 tttctaaaaa acatatcatt gaatctgtag aaaaaagttt gacccgtctg aaaatggatt
    10981 atgtggatgc tttacttctt catcgtccag atacattggt agaaccagag gaagtagctg
    11041 aagcgtttga tgaattggaa cggtcaggaa aagttcgtta ttttggcgtc agcaatcaaa
    11101 aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca accattagta gctaatcagc
    11161 tgcaatttgg cttgaaacat actggaatga tcgatcaagg gattcatgtc aatatgacag
    11221 atgaagcaag tatcgatcat gacggtagtg tacttgatta ttcccgattg catgatatga
    11281 cgatccaagc atggtcacca taccagtatg gattttttga aggtgtattt atcgggaacg
    11341 aaaaattccc tgaattaaac caaacattag aagaactagc agctaaatat caaacgacac
    11401 caactggctt ggcttctgct tggattttac gccatccagc caatatgcaa gtgatcgccg
    11461 gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc ttcagaaatt aatatgtctc
    11521 gggaagattg gtacgaattg taccgtgctg ctggaaacat tttaccttaa agattgtcat
    11581 aagcaggtca aaagggctat aaattaaaaa gacagaataa cttgaaagct gttttcagct
    11641 tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt atcattgtac ggatttcgtt
    11701 tattagttat ttttgttttt aaggaaaagc tgtgtccgat attcttttgg agaaatccct
    11761 tgttttttct taaataaata actgaaatag ttagggtcat tataaccaat ttcaaaagca
    11821 atatctgaaa tacgatagtc tgattgctgc aataattttt ttgctaattc gattctttgt
    11881 tctgtcagat aatcgatgaa tgtttggtta aacgattgag aaaaaatcgt actgaaatga
    11941 gagggactta atgcaacttg ttgagcgacc caagttaagg acatatctgg atcggtaaaa
    12001 ttttctttga taaaatccaa tgcatggttc aaaattggct gatatttgtt ccttgaaggc
    12061 tgtgagggca gagcagttaa gaaagaaatg aacatataaa ctgtcgaacg gtaaagcgag
    12121 tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc aatgaaccat ttcttgcggg
    12181 attttctttt ctttcaggca gacatgctgg agtagcttag tcaagacaaa taaacgatat
    12241 aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt gttcgatgta cttagatact
    12301 tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa agtaaaagac agaatcaact
    12361 ggaagtttcg cttcttgaat caaatcctca tagctgataa tttgttcttt aagggaactg
    12421 gcggtttcaa gtatcatctg cgtgatctca aaagatgcag gtaattcgct gattctttct
    12481 gcgacaggac caatggcgac agaaaaatta tttttttgtc ccttttctaa ttcatggacc
    12541 aacgtttggg ctacctgata actggtctcg atcacatttt cttcaagaga atcaactatt
    12601 aaaaatttga tgaatcgaga ggaaacagca gagaagatca cttgttgatt ctccctaaat
    12661 aaaaacttca aataatcact gagatgagcg taatcttcaa agtctgtact gacttggttt
    12721 gctgctaata atacggtcat ttttttccct gctaaagagc gattgaaaga ttcagcaagt
    12781 ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac cgttcaagaa atgatttttt
    12841 ttcaattcga aaacaagatc agaagaagtg tgcgtttctt tttgctgcaa atctaaagca
    12901 gttactgctt tttctattgc ttgtttcaat tcgctacttt taatcggttt aagcagataa
    12961 tcatctgcct gtacctggat tgctcctttc atataatcga aatcgtcgaa tccactaata
    13021 aatatgagac gagtccaagg aagtagtttc cttgcttctt ttgaaaaagt caacccgtcc
    13081 atgaaaggca tttggatatc agtcagaatg atgtcgggct gtaagtcgat aattgaagac
    13141 agtgccatct caccatctcc agcttcgccg acataagtga ttggataata agaaccaagg
    13201 acagttattt gttcccgcaa gttattgcga atgaggtatt cgtcttcgat gataaaaact
    13261 ttatacatgt ttgtttcctc caattgtttt gggagataga gggatcatca gtgtgacagt
    13321 tgttccttct cggtatttgc tgtcaatcgt gataggtttt tgatcatttc cgtaataaag
    13381 aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata ctgaaatctg aatcgatccc
    13441 tttggctaac tctttacgta tttctgccag tttttctgca gggatgccaa tcccgttgtc
    13501 tataactttg aattggatca tctcatcaat tttgcatgtg gtaatacgaa taaatccttt
    13561 gtgacggacg tacttcgttc catggtatac agcattttca atcaatggct gcaagatcat
    13621 ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg atcttatagt caagcatatg
    13681 gccgtatctt atttttaaga tgactagata atcattgata tggctaattt ctttttcaag
    13741 aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa aagccggaaa gtgcatgggt
    13801 catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa gaaataatgg catccaaact
    13861 gttataaata aaatggggag taatctgagc ttgcaataca cgaacttcac tttgtgctaa
    13921 atggtattgt ttcaaagcgt tctcccgtaa agactggttt agatcttccg ccattttatt
    13981 catgctagcg gtaagtgttt gtaattccag tgtttccggc agggttaaac gaaagccaag
    14041 gttccctgaa gaaagttgtt ctgacatctc gatcagctga ttgatcggtt cttggacctt
    14101 tctctctaaa aagcgacgat tctttttcgc atagaaaaaa atggtactaa gaactccaag
    14161 ttccactatt gttaatagaa tgagcgagcg gacaagtgtc gtattttttt tgcttgctaa
    14221 gtcgatctcc acacgaacaa aatcttgtaa gatatctgac agcagttgag tgacagaatc
    14281 tacctgattg atgatctctt cattggtatc cataaaatct tttgccaaga tattattgat
    14341 cagctcgttt tgataatctt ccagtgtatc gatcgtacgt agcgatacat ctaacgtact
    14401 aatttcttta agtgtatttg tattattttt gatctctcga atatcattcc taagttcttc
    14461 taggatattt tcctcattga actcatctac ggttacttgg ccagaaatca agtcccacat
    14521 actttccaaa actttttgat caaccttcaa agcaattgca tctgcttttt ggatatttgc
    14581 gatcgtctgt tgataaatca aaagctgttg cgaatataac agtatgctca gtagcaaagg
    14641 gataacagca aatagaagca tggctttgtt tgcttgggca aaaatctgtt ttatgcttaa
    14701 attttttgtc atgatacttc tccagtcaat aatttctggt tgggatgtta gctatattct
    14761 gctgggaaaa gacagtctcg gaaataaaaa tatctttagg taatgttttt cctgtttgaa
    14821 tatttccaaa ataacgagta acggtattgg ctacatacca gccgacatca ggtttacact
    14881 cgactacaca attgacgatt cctgagcgta gcttctcgat catatcttct tgtccatcaa
    14941 tagttacaat gatcaaatct tctccaggtt tgattgatgt ttcttttatc gcagctaaag
    15001 caccgtgagt catttcatca ctatggctgt ataatacatc gatttcggat aaatcatttg
    15061 tttctaaata tcgtttcatc tggtcttttc ctttcatccg aataaaatca ccgacaattc
    15121 ggtgaacaat agtcatcgga ggtttatggt cgatggttcc atcttttttt agggttggtt
    15181 tacgattgat tgattccaaa aatccttcgg agcgtagctt ggtcggtgtg gagttctctg
    15241 aaccagcaag ttctaggatt ctgatttcct gtttctttga atttgtgaag tgattgctga
    15301 caaataaacc cgcccgattt ccttctgctt tgaaactggg gccaatgtgg gtgacgaaaa
    15361 gctcgggatc attcgtttga atatgtctgt cgacaagtat gacagggatc cctgctcgct
    15421 ttgcttcttt taatacagag tcccacccgg attcttgcaa aggagtaaaa atgatcatat
    15481 ccactttata ggcaatgaac gtccgtatat cttggatttg ccgttcctga ttcatgtagc
    15541 catttcggta gagaacttgg taatcttctt tttcaagcgc agtgataatt gattcagtat
    15601 gctttttccg ccagctactc tccgttcctg attgcgaaaa accaacgact agtgggtcag
    15661 gtgcttcctt tgtattaggt gtacacccca tcatttggta agcgattaca acaagggcta
    15721 aaaaaaataa aacacgtttc actttaaatc actccatctg gatttgttag tactatctta
    15781 tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg aaaaaaatcc aagaaactta
    15841 aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa agtaaatccc ataaaaaaat
    15901 caatgttaac gctttcttta ttagctatac tcggtgtatc aattgaaatg ggggataaaa
    15961 aatgaaaaac aaatttagaa agattgttgg gactatcgct attttgaccg caagcattct
    16021 attaagtgcg tgtggcaatt caggatctgc agatgatagt acggggtatg taggaatcgc
    16081 tatgccgacc aaatcagcag aaagatggat cgctgatggg aacaacatgg tctcggaatt
    16141 agaaaaactg ggttataaaa cagatcttca atatggagaa gataaagtag aaaatcaagt
    16201 agcacaaata gaaaatatga ttacaaaagg ggtagataca cttgtgattg cttctatcga
    16261 tggttcggct ttaactgatg tgttagctaa agcaaaagaa gcagatatca aggtgatcgc
    16321 ctatgatcgt ttactgatga attcagaaaa tgtcgattat tacgcgactt ttgataattt
    16381 tggagtaggt gtatcgcaag cagcttatat tgaagagcac ttaggattga aagaaggaaa
    16441 aggaccattc acgattgaat tatttggtgg ctcgcctgac gacaacaacg cactaatcaa
    16501 ttacaacgga gtgatgtctg ttttacaacc atatatggac aacggtcagt tggtcgtgcc
    16561 atctggacag acgagtttca gtcaaatcgc tacgcttcgc tgggatggtt ctacggcgca
    16621 agcaaggatg gataatctgt taagcgcaaa ttatactgac caaacattgg atgctgtttt
    16681 gtcgccatat gatccaataa gtttaggaat catttcgtca ttaaaaggcg tggggtatgg
    16741 gagtgaaagt aaacctttac ctgtgattac tggacaagat gcgacagttg ctggcgttaa
    16801 atcgatcatt gccggggaac aaacacaaac gatctttaaa gacacacgta tcttagcaaa
    16861 aaatacgatt gaaatgatca aagcaatcag tgatggagaa gaggttccgg tcaatgatac
    16921 tgaaacttat gataatgggg tgaaaactgt ccctacgtat ttagcaaata cagtatctgt
    16981 cgataaagat aattatcaag cagagctgat cgacaccgat tattataaag aatccgatct
    17041 gaaaaattaa gaggaaccaa acaaggaact aaagagatct aagtcagaga gaggataaga
    17101 attatggcgg attgtatttt aaaaatggca gatatcacca aaaaattttc tggaatccga
    17161 gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa tccatgcatt gtgcggagaa
    17221 aacggagcgg gaaaatcaac attgatgaat gtcttgagcg gcttgtatcc atatggctcc
    17281 tatgacgggg aaatctttta tgatggaaag ctgtgtcaat ttaaagattt gaaagatagt
    17341 gaagaaaaag gcatcgtcat cattcatcaa gaattagcac taagtcctta tctttcaata
    17401 aaagagaata tttttctcgg aaatgaacaa gcagaaagag gcgtgattga ttgggaactg
    17461 acagaaaaga aaacatatga actgctgaat aaagtcggtt tgtctatcga cccgaatctt
    17521 ctagtatccc agattggcgt agggcaacag caactagttg aaatagctaa agcattatca
    17581 aaatccgtcc gtctgttgat tttggatgag cctaccgctg cattgaatga agaagaaagc
    17641 gcgaatttgc tgagattgat ccgagagttg aaagagcaag gtgtaacctc catcattatt
    17701 tcacataagc tgaatgaaat cgtggatatc gcagatcgaa tcaccatttt acgggatgga
    17761 cagacgattg agacactaga aaatgaaatg atcagcgaag agcatattat tcgcggaatg
    17821 gtgggcagag atttagttga tcgttatcct gaaagaaaac cggatattgg agaagtttat
    17881 tttaaagtag aagactggac ggtctatcac cctcttgatc acgagcgaat cataaacgac
    17941 cacatcaact tgcatttgaa aaaaggagag attgtaggaa tagctggact gatgggagcg
    18001 ggcagaactg aattcgctat gagtctattt ggacattctt atggaagcaa gatatccgga
    18061 cgtatattaa aggaaggggc agagatatca gttaagaccg ttccttcagc cattgcaaac
    18121 ggactcgctt atgtctcgga ggaccgaaaa tctttaggac tgaatctgct gatggatatt
    18181 cgggaaaacg tgacgctgag cagtttggat aaaataagtc aaaaaggagt attagataaa
    18241 gaaaaagaag taactgaggc agaaaatttc cggaaaaaga tgcgtataaa aacaaactcc
    18301 atccatcaaa ttgtcagtag tcttagtggt gggaatcaac aaaaagtcgt tttatccaag
    18361 tggctgatga cacaacccga tattcttttt ttggatgagc caacccgtgg gatagatgtt
    18421 ggggcaaaat atgagatcta cacgattatc gaagaaatgg cagcaaatgg caaagctgtc
    18481 tgtcttatct cttctgaact tcctgaaata ttagggatgt gtgatcgaat ctatacgatg
    18541 aacgaaggac gtattacagg agaagtctcg cgagaagatg cagatcagga agtcttgatg
    18601 aaattaatga cgaaagaaga ggtgggttaa gttggaacag gcaaaaaatc ttacggataa
    18661 aaaagagaaa aaactaaatt tgaaaagtag agcagctgat ctttttggtc gctacagcat
    18721 ggtgatcatt ttagcgggac tacttattgc gtttcaattg atgacggatg gtattttctg
    18781 gcgtccattg aatataacta accttgttct gcaaaatagc catatattag tactagctgc
    18841 agggatgtta ttagttgtgc tcttaggtca tgtggatttg tcggtagggt cagtgatggc
    18901 gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc caaatgaacc cgtggatgac
    18961 agttatcgca tgtcttgcag taggcgggct gatcggcgca tggcaaggtt tttgggtagc
    19021 ttatgcaggg ataccggcgt ttatcgtgac tttagcagga ttgctgatgt ttcgtggact
    19081 gacacaagtt gttctaggag gccaatctct agcacctttt ccagctgttt tccaaaaaat
    19141 gtctacgggt tatttaccag acttaagcga aggaagcagc cttcacttct tgacattgat
    19201 tttaggaatg atcatcacga tcgtgttagt tgttggtcaa tggcgaacaa gaaatcgtcg
    19261 gaaaaatcat ttatttgaag tggaatctcc aacagctttc ttggtaaagg cggtatttac
    19321 agcagtcgtt gtactaggaa taacttatat ctttgcctct tatcaagggt atccgattat
    19381 tttgatcatt cttggtttga tcgttgcagc atatgggttt ttgacaaata aaacagtagc
    19441 aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg gctgaactat cagggatcaa
    19501 aacaaagcga atcacgttct gggtattcgt caacatggga gtcatggcag ctttagctgg
    19561 attagtttta gctgctcgat tgaatgcggc aacacctcaa gcaggtacat cgatggaact
    19621 ggatgcaatg gcggcagtat attttggtgg tgcttcgact tccggcggca ttggaacaat
    19681 catgggtgcg attgtcggtg gcttggtcat gggtgtattg aacaatggca tgtcgatttt
    19741 aggcgtcggg gtagactggc agcaggcaat caaaggattg atcttgcttt tagccgttgt
    19801 gctagacttg tataataaga aaaaacgaac aacgtaggag aacgccggtt attcagaaaa
    19861 aagactgtga caagacattt gtcacagtct ttttttcgct aaaagcgaac attcattgtt
    19921 tcgtttgtat agactaaaaa taatatatgt ttttttacca ttcgtataaa ttctcttttt
    19981 gctcttgatt tttttgctta attgagttaa attacatagg gcgctaaaaa aagccttgat
    20041 taaattaata atgttcgtgt tttgctggat gtgaacatca ggaggataat gtaatgaaag
    20101 tatttgatta cgaagatatt caattaattc ctaataaatg tatcgtaaat agccgttctg
    20161 aatgtgatac aacggtcaca ttaggtaaac atacattcaa aatgccagta gtgcctgcca
    20221 acatgcagac gattattgat gaaacaatcg cagaattctt agcggaaaat ggttatttct
    20281 atatcatgca tcggtttgat gaagcagctc gaattccgtt tatcaaaaaa atgaaaaagc
    20341 gtggattgat tacctcgatc agtgttggcg tcaagaaaga ggaatattcc tttatagaga
    20401 agttggcaga agaatcgcta aatcctgatt atatcactat cgacatcgcg catggacatg
    20461 ctaactcagt tattgatatg attcagcata ttaaaaaata tttgcctgaa acttttgtga
    20521 ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt ggaaaatgcg ggcgcagatg
    20581 caacaaaagt cgggattggt ccaggaaaag tatgtattac aaaaatcaag acaggctttg
    20641 ggactggagg ctggcaactg gcagctctta gatggtgtgc gaaagcagct cgcaagccga
    20701 tcatcgctga cggtgggatc agaacacatg gcgatattgc aaaatctgtc cgttttggtg
    20761 cgacaatggt catgattggt tcattgtttg ccggtcacga ggaatctcct ggcgaaacaa
    20821 aagtcgaaaa tggaatcgtg tataaagaat actttggttc tgcttcagaa ttccaaaaag
    20881 gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca acacaaagga tctttgaaag
    20941 atacactagt ggaaatgcaa caagaccttc aatcatctat ttcttatgct ggaggaagag
    21001 atctggaagc cattcgaaaa gttgactatg tcatcgtgaa aaattcgatt tttaatggag
    21061 acacaatcta aaaaataaat aaaatattag gatgttgtga aaaagttgtc ctgcattaag
    21121 taataagaac agccaaacaa aaatagttct ttatatgttt tgtttggctg gaattattat
    21181 cgcagataca agcttttgaa catcattatt cagaataagg gactgtgata agatttttgt
    21241 cacagtccct tatgttttgc aaaaaaagtt tttctttttt ctcgtaaatt cggtaaaatc
    21301 aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg gaaaaagtag agaaaaaaca
    21361 aggaccgatt tatcgtcaaa ttatggaaca gattatgaga gaaatcgaaa acggaaggat
    21421 caatcctgga agtcaacttg aatcagaacg aaaactaggg caactttttg gtgtgaatcg
    21481 ttccaccgtt gtccatgcat tggaagagct tagggagctt ggggtactaa cgagcaaacg
    21541 tggcagcgga tggtttgtta atcagactga atgggggaaa ttcgctgttc caagaatcga
    21601 ttggcggcaa atgatctctc ctcgttatga gcagacggac tggtatgagc aaaaaataag
    21661 agaagcaaag cagatccaaa aaacatcgtt tcttgattta tacgctagcg agatgccaga
    21721 tgagcttttg ccgaattttg agtttccttc ttattctctt gaagaaatct tagcggaaga
    21781 aaaagaaatg gatcttttag gctatactcc gttacgtaac aaaatcaaac ggtatttgga
    21841 aagtaaattt gaattggcat tttcccccaa ccagcttctt gtgactggtg gcggtcagca
    21901 agcaatcttt ttgattttac aaacgattct ttcagctgga gaggggatag ctgtagagtc
    21961 accttctttt ttttaccgat tacctctttt caaagcttca ggaacgcgtt tatttgggat
    22021 accgatggat gaagaaggga tcgacttaga aaaactgaaa gaatcgatcc ataaaaataa
    22081 attgaaagca gtgcttatca acccgaattt tcaaaaccct acaggaaaag tgatgagtca
    22141 aaaacgacga gaaaagctag tagaattgtg tcgacagtat cggcttccaa ttattgaaga
    22201 cgatgtattt acggatcttt catttctaaa ccaagaagaa gtccttccta tacgaacgat
    22261 tgatccggat aatgtgttat acgtcggttc gttatctagg gttttaggaa aaacaacgaa
    22321 aatcggttgg gtgataggac ctgagacttt tatctcgcaa ctagctcaag cgcaagaaat
    22381 gatggagttt tcgatgaata tccttacaca aatcacagtg gctaatgtat ttaatgaagc
    22441 aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa gaaaaaagcc gactgataag
    22501 cgcttgggga gattcacaat cgtttttcaa gcttcattca attttaggtg gttactatgc
    22561 ctggattact tgggatggga aaaaactaga ccccgaacta gcagaaaaag tagtagatat
    22621 gggtcttgga attgcaccaa gtctactttt tgggcaagat ataaatggaa tcagaatcaa
    22681 ttacagtagg ctggatgaaa accaaatacc gttgttttat tcaaagatga aaattctaga
    22741 aaagtggtta acagaataat ttcacaaagt aaatagatta aagaatataa cagcttggat
    22801 ttaaatttca agctgtttct tttttttcgg aaaaagaata cgatataaag aaactaagaa
    22861 aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa aataaatgtt tgcaacaaaa
    22921 ctgtataaca ccaacgtttt taaatagaaa cagatggttg taagtttgat tagaaaatag
    22981 aaaaaatgca gatgaaatat aaaacgcttt ccttattgtg attttattca ctaatgctgt
    23041 taatatgcgg attggcataa tttcctttca tcgtgaaaat ttgtgctttt agcttgtatt
    23101 tctcataaat ccatgataat ttattaatga agacggttaa atagtgatac agttttattc
    23161 actgtttcaa tcgcagaatg aagagatttt cttatagatg agagaggaat gtgtgacaaa
    23221 tggcaaacaa aaaagcaccg atcgacttcc aagcactgct agatgaagtc aatgcagatt
    23281 tcccagtata tcaagcactt gatcaagatg gaaacgtagt taacaaagat ttagtacctg
    23341 atttatccga tgatgaactc gttgaattaa tgactcatat ggtatggtct cgtgtattag
    23401 accaacgttc aacagcgctg aaccgccaag gacgcctagg cttttttgca ccaactgctg
    23461 gacaagaggc aagtcagctg gctagtgctt acgcatttga taaagaagat gttcttcttc
    23521 caggttatcg ggatgtacct caactagtaa aacatggttt acctttatct caagcttttt
    23581 tatggtctcg aggacatgct gcaggtaatt cttatccgga agaattgaaa gctctaccac
    23641 cacaaattat catcggtgca caatatgtac aagcagccgg tgttgcatta ggtttgaaga
    23701 aacgaaacaa gaaaaatgtc gtcttcactt atactggtga tggcggttca tcacaaggtg
    23761 atttttacga agcaatcaat ttcgctggcg cttaccatgc aaatgccgta ttttatatcc
    23821 aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca aactgcagca aaaacacttg
    23881 cacaaaaagc tgtcgcagct gggattcctg gtattcaagt cgatggaatg gatccgcttg
    23941 cagtatatac tgtatctaaa atggcacggg aatgggcgat cagtggaaat ggaccagtcc
    24001 taatcgaaac actgacttat cgttatggac cacatacact atctggtgac gatccaaccc
    24061 gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa agatccattg atccgcttcc
    24121 gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa agaagaacaa gtcatcgaag
    24181 caactaaaga agaaatcaaa gcagctattg ctgaagccga caaagtacca aaacaaaagg
    24241 tttcagattt cttgaaaaac atgttcgaag tatctccaca atcaatcaaa gaacaaattg
    24301 caatttatga agcgaaggag tcgaaataat catggcacaa aaaacaatga tccaagcaat
    24361 cacagatgcc ttagcacttg aactagaaaa tgacgagaat gtcgttgttt ttggtgaaga
    24421 tgttgggaaa aacggaggag ttttccgggc aactgaagga ttgcaggaaa agttcggaga
    24481 agaccgagta ttcgatactc ctttagctga atctggaatc gcaggattga gtttcggtct
    24541 tgcgttagaa ggatttcgtc cagttcctga aatccaattc tttggtttta ttttcgaagc
    24601 aatggacgaa gtagtagctc aaatggctcg tacaagatat cgaatgagcg gcactagaaa
    24661 tctacccatc acaattcgtt cgccatttgg tggcggtgtt catacaccag aattgcattc
    24721 ggataattta gaaggtttga tcgctcagtc tcctggtatt cgagtagtga ttccttccaa
    24781 cccttatgat gcaaaaggac tattgatttc ttctattaga agtaacgatc cggttgtttt
    24841 cctagaacac atgaaactat atcgctcatt ccgagaagaa gtgccggatg aagcttacga
    24901 agttcctttg gataaagcag cagttactcg tgagggaaca gatgtatcta ttatcacata
    24961 cggagcaatg gtgcgtgaag caatcaaagc agcagacaat cttgcaaaag aaaatatatc
    25021 tgttgaaatc attgatcttc gtacagttgc gccattagat gtagaaacga tcattcaatc
    25081 cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca caacgacaag cgggcgttgc
    25141 cgcgcaagta gtttctgaga tctcagaacg cgctatcctt tcattagaag ccccaatcgg
    25201 acgagtttct gcacctgata cagtcttccc atttggacaa gcagaaaatg tctggttgcc
    25261 aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg gaattttaag aaaagaaggg
    25321 acgaaacaaa gtcatggctt atcaatttaa att
//
LOCUS       DACEPD010000038        25325 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.038, whole genome shotgun sequence.
ACCESSION   DACEPD010000038 DACEPD010000000
VERSION     DACEPD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.038"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            237..1877
                     /locus_tag="IW797_001435"
     CDS             237..1877
                     /locus_tag="IW797_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ1224219.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2031..3353
                     /locus_tag="IW797_001436"
     CDS             2031..3353
                     /locus_tag="IW797_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1224220.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3415..3846)
                     /locus_tag="IW797_001437"
     CDS             complement(3415..3846)
                     /locus_tag="IW797_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1224221.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3910..4746)
                     /locus_tag="IW797_001438"
     CDS             complement(3910..4746)
                     /locus_tag="IW797_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ1224222.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4936..5238)
                     /locus_tag="IW797_001439"
     CDS             complement(4936..5238)
                     /locus_tag="IW797_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ1224223.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="IW797_001440"
     CDS             complement(5264..5695)
                     /gene="ruvX"
                     /locus_tag="IW797_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ1224224.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5692..5958)
                     /locus_tag="IW797_001441"
     CDS             complement(5692..5958)
                     /locus_tag="IW797_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ1224225.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6212..6937)
                     /locus_tag="IW797_001442"
     CDS             complement(6212..6937)
                     /locus_tag="IW797_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ1224226.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7303..7373)
                     /locus_tag="IW797_001443"
     tRNA            complement(7303..7373)
                     /locus_tag="IW797_001443"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7339..7341),aa:Gly,seq:ccc)
     gene            7490..8242
                     /locus_tag="IW797_001444"
     CDS             7490..8242
                     /locus_tag="IW797_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ1224227.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8258..8737
                     /locus_tag="IW797_001445"
     CDS             8258..8737
                     /locus_tag="IW797_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ1224228.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8988..10568)
                     /locus_tag="IW797_001446"
     CDS             complement(8988..10568)
                     /locus_tag="IW797_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ1224229.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10561..11304)
                     /locus_tag="IW797_001447"
     CDS             complement(10561..11304)
                     /locus_tag="IW797_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224230.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11480..12256)
                     /locus_tag="IW797_001448"
     CDS             complement(11480..12256)
                     /locus_tag="IW797_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1224231.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12504..13340)
                     /locus_tag="IW797_001449"
     CDS             complement(12504..13340)
                     /locus_tag="IW797_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ1224232.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13351..14280)
                     /locus_tag="IW797_001450"
     CDS             complement(13351..14280)
                     /locus_tag="IW797_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ1224233.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14472..14825
                     /locus_tag="IW797_001451"
     CDS             14472..14825
                     /locus_tag="IW797_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1224234.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14899..15555)
                     /locus_tag="IW797_001452"
     CDS             complement(14899..15555)
                     /locus_tag="IW797_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ1224235.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15781..17715
                     /locus_tag="IW797_001453"
     CDS             15781..17715
                     /locus_tag="IW797_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1224236.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17801..19024)
                     /locus_tag="IW797_001454"
     CDS             complement(17801..19024)
                     /locus_tag="IW797_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ1224237.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19018..20916)
                     /locus_tag="IW797_001455"
     CDS             complement(19018..20916)
                     /locus_tag="IW797_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ1224238.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(20910..22028)
                     /locus_tag="IW797_001456"
     CDS             complement(20910..22028)
                     /locus_tag="IW797_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ1224239.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22111..22944)
                     /locus_tag="IW797_001457"
     CDS             complement(22111..22944)
                     /locus_tag="IW797_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1224240.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22937..23854)
                     /locus_tag="IW797_001458"
     CDS             complement(22937..23854)
                     /locus_tag="IW797_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1224241.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24081..25205)
                     /locus_tag="IW797_001459"
     CDS             complement(24081..25205)
                     /locus_tag="IW797_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1224242.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISIADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT         7809 a         5047 c         4308 g         8161 t
ORIGIN      
        1 ttctatttat ctatctaaca tatcctttat tcttctactt ttctttcttt agcatataag
       61 agatacgcat agtaatcaag caaggattgt tgatataaaa agaacaaata ccgctatttt
      121 gatttctttt gactctatta ctcatataaa taaaatttat tatacctgct ctttttgact
      181 atatcctcat ctgactccta tacttcgttt gtattaaaac ttaggaggaa tcattcatgc
      241 ttacagggct aaacttatta aataatcctt ttctaaataa aggaacagct tttacaaaag
      301 aagaacgggc aaaatacgga atcactggta tgcttccaag tactgtccaa acattggaac
      361 aacaatctgt ccaagcttac ggtcaatact taagcaaaca gaccgatttg gaaaaacgta
      421 tcttcttaat gaatctattc aatacgaacc ggacattgtt ttataaattg atgggcgaac
      481 atttagttga attcatgcct gtcgtttatg atcctgtcgt tgcggattca atcgaacaat
      541 acaacgaaat tttcttgaaa ccacaagatg ctgtatttct ttcaatcgac gaaccagaaa
      601 atatcaaagc aagtttgaaa aacgcagcag atggacgcga cattcgtttg atcgttgtga
      661 cagatgcaga aggaatctta ggtatgggtg actggggtgt gaacggtgtt gacatcgcta
      721 tcggtaaatt gatggtctac actgccgcgg ctggtatcaa tcctgcacaa gtcttgcctg
      781 tatcaatcga cgctggtact aataacgaag aattattgaa taatccatta tacttaggga
      841 atcgtcatgc tcgtgtagaa ggcgaaactt actacgaatt cattgatcaa ttcgttcaat
      901 cagctactga attattccca gaattgttat tacattggga agattttgga cgaggaaatg
      961 ctgcaacgat cttagaaaaa tacgaagata aaatcactac tttcaatgat gatatccaag
     1021 gcacaggaat cgttgtacta gctggtgtat taggtggatt gaatatatca ggtgagcaat
     1081 tgaaagatca aacgatcttg acatttggtg ctggtactgc tggtgtaggt atcgcaaaca
     1141 tccttttaga tgaaatgatc cgtcaaggtg tacctgaaga agaagcacgc caacatttct
     1201 atcaagtaga taaacagggt ttattgtttg aagatacaaa aggattgact cctggtcaaa
     1261 tcccattcgc tcgtaaacgc tcagaattca ctaatgctga tgaattgaca aacttagaag
     1321 cagttgtaaa agcaatccat ccaacgatca tgatcggtac ttctacccaa cctggagcat
     1381 tcactgaaga aatcgtgaaa gaaatggcgg ctcatacccc acgtccaatc atcatgcctt
     1441 tgtctaatcc aacaaaattg gcagaagcaa aagcaaaaga tttgattgaa tggacggacg
     1501 gaaaagcttt agttggaaca ggtatccctg ctgctgatgt ggaatataac ggtgtaactt
     1561 accaaatcgg tcaagcaaat aatgcgttga tgtacccagg tttagggctt ggtttgattg
     1621 cttcaacagc tacacgagta aatgctgaaa tcatttctca agctagtcga gcattaggcg
     1681 gaatcgttga tgtaacaaaa ccaggagcag ctatcttgcc tccagtagca aaaatcactg
     1741 aattctctca aacgattgct gaaactgtcg caaaatcagt tgttgcacaa aaattgaatc
     1801 gtgaagaaat cacggatatc aaagaagctg ttgaatctgc taaatgggtt cctgaatata
     1861 aatctttaga agattaagat tgttatagta aaagaacata cagaaacttt gtatgacgtc
     1921 taaaaagaaa aaagcgactg cggcaaaaca gcagccagcc aactactgct cttttgccgc
     1981 ttctctttgg cttttttcat acgatacaga ataagaaagg atctgtaaaa atggcacaaa
     2041 aaacaacaac cgcaactgca gttgaggaaa agaaaaaaga agggttttgg tcaacaaaaa
     2101 ttaccggcgt aagtttgccc ctttatttga tcatggtcct tgtcctgatc attgcaatgg
     2161 ctttaggaaa attaccgacg aatatgattg gcccaatcgc tgttttggtc attttcggta
     2221 atttatttca ttttatcgga acaaaaatcc ctatagtcaa aagttattta ggtggtggct
     2281 ccgtttttgc gatctttgcc tctgccgcga ttgctacatt tgggatctta cctgagtatg
     2341 tagtaaaatc cacagaaaac tttgtcagca atatgggctt tttagacttt tatattgctg
     2401 ccttgattac tggtagcatc cttggtatga accgtaatct attgatgaaa gcttctgttc
     2461 gttttattcc cgttgcactt atttcaatgg tcgtctcctt ttttgccgta ggactcgtag
     2521 gtatgcttat cggaaatggt tttgcaaatt ccgttttata tatctcattg cccgcaatgg
     2581 ctggtggtgt tggagccgga gctgtacctt tatcaacgat ttatgcaaat gttttaggta
     2641 cggacgcttc ttctatcatt tcccgcctga ttcctgcgac agcaatgaca aatgtcctag
     2701 ctatcattgg tgcagcgtta gttgctcgtg cgggacaatc aatgccaaaa ttcaacggta
     2761 atggtaaatt aatggaaaaa ggcgaccttg gcgatggaaa acctagaaat gtaaaacctt
     2821 ccattccaca attaggtgtc ggtttgatgg tatcattgac tttcttcatc ttaggacaaa
     2881 tctgtaacta ttttgttcct aaagtccatg cttacgcatt catgatcatt atcgttgtta
     2941 tctgtaaaat cgcaaaccta ttaccagaat attacgaaga tgcagctatc atgttcaaca
     3001 acttgattgt caaaaacttg acagcagctg ttcttgccgg tatcggtatt gcgttattga
     3061 atctaaatgt attggcttct gctttgacat ggcaatttgt tgtcttatgt ttgacaagtg
     3121 ttatcgcgat ttcagtagtt tcaggatttg tcggtcgttt gtttggttta tatccaatcg
     3181 aatcaactat tactgctggc ttatgcaaca actcgatggg tggtacagga aatgtagctg
     3241 tcttatctgc agctaatcgg atggaactta ttgcttttgc tcaaatgggc aatcggcttg
     3301 gtggtgccat cgttttgatc atctcaggtt tcttgatgca attactttct tagtcaaaaa
     3361 aaatacctga tcatcaagta tcggcttgat gaccaggtat tttttgataa aaatttacag
     3421 gattttcaaa aatctttttt gcattttatc ggaatgataa ccgatgcgtt cataaaatcg
     3481 gtgtgcttct actctgtatt ctgcagaatt aagtcttatt gctgatattc ctcgtctttt
     3541 cgcttcttct acgccagtca tcaaggcctt tccgattcct cttttttcag catgtttaga
     3601 cacagcaagt gccataatat tgaacatcgg atcgctgaaa atcgattcat aggactccgc
     3661 ttgtacatag ccagctgctt ttttagaagt aggatcttca tagaccaaga aaaaattttt
     3721 ttctggctcc ttcaacagtt tcttcatttg tctcaccgta acggttaatg gtacatcata
     3781 tcctaattgt tccgcattga tttctcgtag tgcttctgca tctcctaaac gcatttcccg
     3841 caacatccaa atcaccagcc aatttttatt tttttaaatg aattttttta tcacagtata
     3901 ctcctttttt cacttaaaaa aagacttttc agtgattttg acttgcttga atccagatcc
     3961 ttctaatgtt tgcaagatga cgaaagcatt gtaaagattg atcaaaagaa ccacgatgac
     4021 aatcgcttga aaaataaagg tcactttttt tggagacaaa accctgctaa atcttgctcc
     4081 ccacaaacct ccaataacag cagcggggat aacataaaac aacatagata aatcaaagcg
     4141 cataaagcta cttgttaaag caatcgttac caattttgcc agttgagaaa agaaaatcgt
     4201 agatagagaa tatagtgttg cttcttttat tggcaatgca aacatcagca tcaacaaaga
     4261 aacattgact ggtccaccac caatacctaa aaaactagct aaaaagccca gaaccccccc
     4321 acatataagg taccaagtcc atgacgtcaa atgaaatttt ggctgatgat gcttggtgta
     4381 gaagaacgca aaaaccaagg tcaataccgt caaaaaaatt tggatcagct gcacatgctt
     4441 ctcgttttca aataactgta aaaaaacttc gaatacaatg ttgccgacta ctcctccaac
     4501 aacagctcca ctagataccc aaccgacaat ctgccaattc agtgatttcc cgcttgatac
     4561 ttgtcgcaca gtcgaaacaa tggacatcgt gaataccgca actgttgagt aaaaagaaat
     4621 ccctgcaaca gaatgggcgc cgataagatc aagaatcggt ttgatcaaca cgcctccacc
     4681 catcccggaa atagcgccaa ctgtgttcgc cagaacgatc accacaaaat aaatcagacc
     4741 aatcatcttg tatttccttt ctcttgttta tatcttttag cgattgttac tcttttcttt
     4801 tattatacag taaaagaaac aaacgaggct tcgaaagctt ttctactttt taaggaaatt
     4861 tagtgctaaa cgaaaaaacc gggctctact tttcagagag tccagctttt cttacacctt
     4921 atcgattctt ttgttctatt cttcttccgc catgaatgta ttgaatactt cttcgatcat
     4981 atcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     5041 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag ctggatataa
     5101 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagcaaga tttcgtataa
     5161 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcgt gttcgtggga
     5221 atggttatgt tctgtcataa gattacctct tttcttttta ttattaaaat aattgcttac
     5281 ctgagccgtc taaataattt tgtaaaatca tcacagctgc taatttatcg atgacttttt
     5341 ttcgtttcgc tcttgaagta tttgcctgct ctactagcat acgttctgcc tgaacagtgg
     5401 ttagtcgttc atcttgataa tcaaccggca aggagaatag ttctttgatc ttctctccat
     5461 atgacatgga agcttctgcc cttggtccga tggaattgtt catattctta ggtaatccaa
     5521 ctacaaaacg gtctacttcg tactgcgcca ctaattcctt caaccgatca aaaccaaact
     5581 cttcttctgc ctcattgatt cgaatgattt ctactccttg agctgtccag ccaaagggat
     5641 cgctcacagc aacgcctact gtcttagaac cgacatccaa gcccattact ctcataaatt
     5701 cactccatga tttgagaggt agtctttgac aattacttcc atgatttcat cgcgttcgtg
     5761 acggcgaatc aaatttcgtg catcacgata acgaggaatg taggccgggt ctccagaaag
     5821 caagtagccg acaatctgat tgattggatt gtagcctttt tcttccaaag cccgataaac
     5881 aatcgctaat gtttcgctca cttctttttt gcgactatcg tcaaaaccaa aacgtacggt
     5941 ttcatctgta aaacccatga ttctacacct ctttcccgca aggtaattcc agcttacctt
     6001 ctacaccctt attttactcg aagaaaggga aaactacaaa caaatattcc aaacttctga
     6061 ataaattaag aaacacgatt tgtctgattt ttcctttttt ctgtggcttt ttcaaaagcc
     6121 ctctttccat aaagtatacg ctatatttct ttgaaagtat gtttttacac aattttttca
     6181 taaagttcat aaaaaattcg aattgctgct actaattttt cgttttcgtt gcttgccctg
     6241 tctcataatt gatggtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     6301 ttttgtcttc aatgctttct gcttccaact gcacacctct tgctaccagt tcatttccct
     6361 cgaaatgagg agtgacacga tagcgtacat gatgatttgt ttccttgata tatgccgcaa
     6421 tatcattttc gtgggcaagc atgtacggag cattcaacga acgtgtccct gtcattaagt
     6481 ttttaatgtt attattttga ccagtcaact ggaaaccaat caaatggctg cggttgtata
     6541 accattgtcc gtcacttaat ttcttttgtt tccaacctgt cggatcaata tacaaaggtt
     6601 ctctcttttc agtaggaaac aagtcttttc ccaacatcgc attggcttgt cctactctgt
     6661 tcaatttgtc tatatttgaa tagctttccc atgtcccttt ggaaagacta agatcctctt
     6721 ttgtaaactc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     6781 gcacatccgc tacttgagaa gcttgcggtg tggaatcaaa aatggttgta atttgttgga
     6841 agatatctga atctttttgt gtggtggaaa taccgagtgc ccctgcaatc acagcagcga
     6901 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagt
     6961 tccctaaatc ttaacatatg ttcccctata aataaatact ggaaagaaat agaaacagta
     7021 aaaaaataaa accagttttt agacggctta agaatacaaa aaaagagacg gattcatcgt
     7081 ctctactcaa tcacaaaaca ctatttacaa ggtatttatt atcattcaaa aaatttttct
     7141 taatactcaa aactaaacgg cttctgtacg acctcttggt aaacagtgtt caaaagcttg
     7201 catctgcggt aataagtcca accaagtcaa aaacataaga agcttttttg cttgactatt
     7261 cttattactt gatgcagggc agctttttca cagcctcttt taagcgggtg acgggaatcg
     7321 aacccgcgac atcagcttgg gaagctggga ttttaccact aaactacacc cgcgatacaa
     7381 aaacagtata cgcctctttc actccttcct caagtttttt tattctttac gcttccgata
     7441 tgttttgtag taaaataaaa ctaatcaatc aattattgga gaagatgcta tgacgggaaa
     7501 agaattagat ctgttgctgg acacttgttt actcgctggg aaaatcatga tggaaagcaa
     7561 tgcggagatg tatcgggtag aagatacgat gagtcgaatt gctttagctt ctggaaattt
     7621 ccgtttggtc agctatgtca ctcaaaccgg tctctttatt ggtcttgatc gtacctcgac
     7681 catccgaatg gaacaaatta ccaaccgttc gattaattta gaaaaagtag taaaagtaaa
     7741 tgatctttca cgtaaatatg ttgcttcaga gctaacatta gaagaacttt actgcgaact
     7801 gcaagcaatc gaccgagacc gttctttctt tcctgtctgg ttgcaaatcg ttagcgcggc
     7861 agtgatcagt ggaacaatta tggtgttatt tggcggaaag atgaccgatt tacctgtgac
     7921 tttagtcatt ggaggattgg gatacgcagc ttatctatat agtttgaaat tttttagaat
     7981 caaattttta tcggaatttt tatcttcttt attgattggc atagccgcta tctacagcgt
     8041 acgttttggc ttaggaagca atcaggatct aatcattatc ggatgtgtga tgcctttagt
     8101 tcctggagtt caaattacga acgctatccg tgatttgcta gctggacatt atgtctcagg
     8161 agtttctcgc ggaacagaag cgatgatgac tgctacgatg atcggttttg cgattgcttt
     8221 tattttccag cttttttatt aggaaaggaa cacaagtatg gggacgatat ttattcagtt
     8281 ttcttttagt tttttagcga ctgcggcatt tgccgttatt acaaatgtac ctaggcgttc
     8341 attgatttgt tgtggtcttt ctggtgcttt tggctggatg atctactgga tctgtgttca
     8401 aataggcgga tctgctgctt ttggttcatt gcttggttct ttaggagtag cattcatcag
     8461 cgatcttttc tctaaacgtt tgaaaatgcc cgtaacgatt ttcaatattc ccgggatggt
     8521 tcctctagtc cctggtggtt tagcttatca agcggttcga aatctcgtta ctgaatccta
     8581 tcgagaagcg gctcattatg cggtccaagc aattatgatt gctggagcaa tcgctttagg
     8641 tcttgtatta tcagaagtct ttaaccataa catccgtaat ttcaaagtaa agcgtgacgc
     8701 aatcatgatg aaacgaaaaa aacggctttt taaataaacg aagtgtaaca aagaaaaggt
     8761 tgtgaaagag acgttcagcc ttgagcgatg acaaaatatc tcacaaacat tgcgacaagt
     8821 aaccgcagga gcaataagaa gtaattatga gtattaaaaa aagttttgta aaaatagctt
     8881 ctcacatttt tacataaaat tgacttaata ctgagaaact gacttttgaa caccctctat
     8941 ctgtaaaaaa ggggctgcga catgattttt gtcacggccc ctcttcttta ttcaaatttt
     9001 ttccaaaacg ttcgttgata ataaacgatc catagagcgg aaagtattag aactaaaccg
     9061 atcacaggaa tattcactaa ccaaaatgga taaaacaaag ccaacattcc gtataagacg
     9121 ataagaataa tactgatgat caatgaaccc atcatagttg cgactaaacc caaacttcct
     9181 gaacctctgt taaataattg gttaatattg gtccagttca gcagcagcaa ccgataatct
     9241 ctagcgaaat aacgtagtga caataagtag cagccgaata aagaaccaaa tagcaagcta
     9301 gggataaaaa acaaaggcag ctgaaaagac agtccgctta ataatgcgat cccaccagtt
     9361 aaaaaactct gcaatatcca tccgaatctg aacttttctt ttaaatattg gttcatagag
     9421 attggcaaag aacggataaa cagaaaattt tcttgatcca aagaaataag attgctgata
     9481 aaagaagtct gattaactgt taagaaagcc aatgcgatcc ctaccaggaa aacaacaccg
     9541 attaacttat tatctaaatc agttaatttt atttcgcctc ctagggcaaa agcaagaata
     9601 aaaatgattg gagttaacaa ggaagaagaa agaacttgca taatcaaatt aggttctttt
     9661 actaattgag cattgtaact aaataaaagc tgatgcacat tttggttctt tttatgggtt
     9721 ctttttattt tgaactcact gtttgaatca gtcagttgct ctgtaagctt tggtactatc
     9781 agctgtttta cagcaaggaa aaatcccaga gccgtgatag ctagtattcc ccaacccaat
     9841 aaaccttgtt gagaaagtgg ctgactggca gcatcataga atggcaataa tatcgaaatc
     9901 ggatgccgat ctgttaactc attttcaata gtccccgttt gatgattcat ccacatgatt
     9961 ccagcaaatg cgatcagcat cgagctgcca agcaagatac tagaaactgt ttttctatgt
    10021 tttttaaaca ctgctgttcg agccaaacca aaaacaatca aactagccag acaaaaaaga
    10081 ataatcaaaa atagagaaaa caaaaggatt gagataatga tagtaagcaa aatccctaga
    10141 tcagctcgcc atccggttaa aataaacatg actagaagcg gaaaaacaaa aggaatgatg
    10201 gtcaaagcaa ctaccaaaat ctttgctgaa aaaacctgtt cttgtccaaa aggcagaggc
    10261 aaaaatactt gcaggtcatt tccttcaaaa aagatattat aaatgactga tatactttga
    10321 gttaaaccta acataccaaa aagtgccacg taataagtaa agaaacctgg caatttaaca
    10381 aagttcatag caaacatagc aaatccgtaa atcagtaaaa aaatcagccc tgaaaaaaga
    10441 ttttgagaca aaagatagat agttaatcgc tttccctttt ttcctttctc tcttgcttta
    10501 ttcgtgagct gtggattaac atataaaaga ttaactcgaa ttaattctaa taattgtcgc
    10561 ttatacatac ggtccatctc ctgtcgcttg ccttccagcc attttcaaat aaatcttttc
    10621 tagcgtttca tcaggtgagc cattcaataa ttcactaact gccccctgat aaatcaattc
    10681 acctttcttc aaaatagcca gtttatcgca taactgttgt gcggtatcca atacatgtgt
    10741 agaaaaaatg actgtttttc cttttgcggc atgagccttc atcatttctt tcaaatcaaa
    10801 tgctgcttga ggatcaagtc cttgcaaagg ttcatccaat acccaaatat ctggatctgg
    10861 cagcaacgtg ccgatcaaaa tcgttttttg acgcattcca tgtgaaaaac ttgcgattgt
    10921 ttcattttga tgactataca tatcaaaaag cgctgtcaat tctgctaaac gcttttcttt
    10981 tttttgtgtg tctaattcat aggcagctga aatcaagtcc caatattctc cagccgtcaa
    11041 ttgtaaaaaa atatctgggg aatcaggcac ataaccgatt ttttgtttga tactcaatcg
    11101 attttccgtc aatttcattc catctactaa aatcgttccc gatgatggtt caataatact
    11161 aactaaactt ttaatcgtag ttgattttcc ggcaccatta tgtcctaaaa atccaaaaat
    11221 ttccccttgt cttatcgaga gagacaagtc ttttaatgct tgttttttcc cgtaactttt
    11281 acttacattg atgaattcaa tcatttctgt actccttttg tttgttatta ctttttattg
    11341 tacccttcgt tattggtgga gtaaatgtca aaaagagtgc ataacaaaaa aggatagaaa
    11401 cgactaggct gctacttggg ttgttctctt ctacaggaaa acactcttct aacaggtaag
    11461 atatgagtct ttgttttctt taaaatgatt cattttctat cttttcttgc ataaaattct
    11521 ttagtttatc aatacttctc tttcccatct ctagttgttc tccatttgca aatgttacta
    11581 tttcgtttac tctgctaata gatgtaatgt tttcagtatt aatgatataa gcttttaaat
    11641 ctttacataa atactcatat tcgagtgatt ttttatattt tttaattgtg ccgtttacga
    11701 gaatctcttc atctttcaat actaggccta ttttattttt ataacctggc atgctcatta
    11761 gataaaggat ttcatcgtaa tggatatagt gatcgctatt accataacgg acagttgtcc
    11821 accttttttt cgtagcatgt tgaacaggat agttttcttt ttctttcaat aagtaacttt
    11881 ggatgctttt tttatttttt tcataagtga attcttttat caaataacca ctaggcgtaa
    11941 tctttcgatt aataacagtt attgctttat tcggatgaga cgttagataa acaattttac
    12001 acttttcatt cttttctcta agcatttcgc ctagttcgat accgctataa taggcgttca
    12061 aatcgatgtc taggaaaaaa atatctgagg aatagatttc attattttta atgaattcaa
    12121 caaattgtat aacgttcaat ccctgaacga tctcaaattt ccaaggactt aataaaattc
    12181 tggattcttc caaaatttct actagcattt tttcaaaaac caaactatca tccaaaataa
    12241 atatacgcgt taccataaaa agtacctcca ctcgatattt tactatcaaa atcatttcat
    12301 acaacttttc aacatacatg ttaggaataa attgtaattc tcttcacaat cttttccaac
    12361 acctttgaaa ccgatatcaa atactttcag gatgctgctt ctaaaataaa tatcaaacaa
    12421 atgaatacat catgcaaaaa aagccagaca accgacgtcc ttgcgtgtca gctgtctgag
    12481 ctatgtttat ttattttgtt cttttaaaaa gaacgataca catactgatt tttcggagca
    12541 gagactttct cagcgtttgt aacaactaca gatgggattt cacgcttgaa tggttgataa
    12601 tagtctgatt ggatggttcc ttctatttta taccattcat catttttaac cgttactcct
    12661 tccggcatat gcgtcaacaa accaaagact cctgaatctg ccacacaatg gataatgccg
    12721 aatcggaaca agaagatatc tgtattgcta tcgttcggtg tttggtaaac aaatccttca
    12781 tagctgattt ttttgccaat gaattcgcta gggtaattat aaattagctc cataacttct
    12841 aaatagtttt catctgtgat cttgatagtt tgcttatccc catacttttc tttcaactca
    12901 ttcatttggt ctaagtagtc tgatttgttg aaataaatac tggtatccgg ctttaagtat
    12961 tgtgtctgca tctccggatc ccctactgat tctttactta ctgggaaatt aaatcctttg
    13021 gcttctacga tcgtagtgtc gagactaact gttggaaaca acgtccccac gattactggc
    13081 aaagctagca gtacataagc gattccacgt tgataaggtt ttgacaaccc atggtcatga
    13141 tcgtgctcgt gtgccttttc ttctgctttt ccgtttttga cccacaaata cagctgaact
    13201 aaagccaaaa gagcagataa aatcattgaa agaatagcta agtagcgata atggacattg
    13261 atatattgat cgagtttgcc agaaacttgc aagtacatca tcaattcgaa atacccagat
    13321 aaaatcaaaa accgcagcat attttccctc ctaaataaat aatgtgaatc caataacgct
    13381 aagggcaatc agtccgatca gcgataaaat aaagcgtcct gtaaaatatc gtttcatcat
    13441 cagtagattt ttgatatcga ccattgggcc aaagaccaaa aaggcagcta caggtgctgt
    13501 gccgaacaga cttaacaaag aagatcctat aaacgcatct gcttctgagc aaagagacat
    13561 cgtaaaggcc aataacagca tgatgataat ggctaaaatc tttgttgagc cgagcgttag
    13621 cataattcta gttggtaaat aagtctgcat cgcagctgcg attaatccgc caatgatcaa
    13681 ataacgacct gtatcaaaaa attcgtcgat tgaatgtgtc aacacatgcc cagtttgatg
    13741 gatcagctga ttttttggtt cattcttctc actttgttct tcttcatgga catgatgatg
    13801 gtgattttct gctactttag acgtgatttt ttctttcaat acagcgtctt tttgaaaata
    13861 ggcaagccag acaccaatca caagggcaac aaaaaagctg cctatcaccc gccagcttgc
    13921 catctgccaa gaattgccaa atgcgataaa agtggaaaaa atcacgattg gattgatgat
    13981 cggagcagtc agcataaaag caaacgctgt gtgaactggt acgccttttt taacaaattg
    14041 atgaacgatc ggtacgatgc cgcattcaca tgaaggaaag aaaaatccta agatcgaacc
    14101 agtaaaaata gagaagatac gattttcagg aagccatctc ttcacacgtt ctggggtcaa
    14161 aaatacttgt aaggcgcctg agataagaca gcctaataat acgaaaggta atgcttcaat
    14221 aacgatcgat aagaaaatcg tactcatctg caaaaccgaa tttggaataa aagaaaacat
    14281 gagtttcctc ctttttattt ctagttcatt cgctttgcaa atatacgctt ttattcaaaa
    14341 aaaacaagtt gctctgtaaa tttttacaca actcttattt tttaccaaag cacttcaatt
    14401 gactttttct gttattccca ttaaattaag gtctcataca tatcacttct atcataaaag
    14461 ggagaataaa aatgaaacaa accactaact tgactgaagt tcaagacata ctgcaacagt
    14521 catccgttgc cgtcatttat ctttcgatgc ctaattgcag tgtttgtcac gctgttcgtc
    14581 cacgactaga ggaacttctc acacattatg atattccggc acttcatttg gatgcatttg
    14641 aaacaccaga agtagctagt aaatttgaag tgctgactgc gccagttgtc ctgatttttc
    14701 atcaaggaaa agaagtcttt cggcaagcac ggttcatcga ctttaaaaaa atccgttatc
    14761 tactagatga actgacagca gaagatgatt ccctttctta tgaagaaatc ttccgaacaa
    14821 aataatcgtt gtccaaacag aaaaaagcga ttgaacaagt gaaagttttg ccttcatttg
    14881 ttcaatcgct ttttattttt attctgctgt attttcggaa tgcgcatttg cgtcagaatg
    14941 caagaaatag atcaaggttt gcaattcatt cgtcaaatca acattttgga tcaatacatc
    15001 tgacggagca ttcaatcgag cagcggtaaa gttcaagatt cctttgattc ctgcttccgt
    15061 taactgattg actacttctt gtgctctttc agcgggtatc gttaaaattg ctacttcgat
    15121 ttgttggatc ttgatttgtt ctgacatatc ttccattgga taaacgggga taccgtctac
    15181 gatgcgccca acgatttcct cattcacatc aaaagcacaa ctgatacgta tgctattact
    15241 ttgatggaat ttgtatttta gtaaagcact tcccaagttc cctacaccga tcagcgcaac
    15301 atttgtcaat tgatcgtcgt ttaatgtctt tgcaaaaaag tgcataagat cttctacatc
    15361 ataaccatac ccacgttttc ctaattcgcc aaaataagaa aaatctcgac ggatcgttgc
    15421 actatctact tgaacggctt cacttaattc agttgaagac acttttttct ttccagcatt
    15481 atttaaaatc cttaaatagc gataatacaa aggcagtctt ttagctgtcg cctttggaat
    15541 aatatgatct ttcacgaaat gacctccacg tattgtctaa ttacatcttt gatttctcca
    15601 tcatagcagt atttatagga aaaaatcaat tccattgctc agatattgtg aaattttatt
    15661 ctttattcat ttatttatca tttctgcaaa tctcgcagta taatggaatt tatggtagac
    15721 tactaattga aaaatagttt tttatttgag gagaattcac aaagtagaaa agaggattcc
    15781 atgatattat tacaagccaa tcagattgct cgccattttg gtgcagaagt cttatttgaa
    15841 aacatacatt tagaaatcag tacaggcgct cgtgtcgctt tagttggcag aaatggcgct
    15901 ggaaaatcga ctttattaaa gatcattgct ggaattgaag cacctgatga aggaaagatc
    15961 gctaaaaata aaacagcgac tctcggatac ctcgctcagg acacaggact tgattccaat
    16021 gaaacagttt ggaacgagat gctgaaagct tttgaacaag ttagacaaat ggaagagcgg
    16081 atgcgcgagc tagagatttt cattagcgaa ggtacgccag acacacctgc ttatagttct
    16141 tatttaaaag aatacgaccg gctgcaacac gatttttcag atcgaaacgg ctatggttat
    16201 gaaaatgaaa ttcgttccgt attgcatggg tttaaattcg attcttcatt ttatgatcag
    16261 aaaatcgata ctttatcagg cggacagcgc acacgtttag ctttagctcg catgcttttg
    16321 caacaccctg acattttgat tctagacgaa ccaacgaacc atttagatat cgagacactt
    16381 tcctggcttg agaattatct gcaaagctat actggtgcat tattgattgt ttcccatgac
    16441 cgttattttt tagataaagt agtcaatgaa gtctatgaat taagcagaag aaaaatgcgc
    16501 cattacaaag gtaactattc gaaatatcta gatttgaaag cagaacagtt agcaagtgaa
    16561 tggaaggctt atgaaaagca gcaaacagaa atccataaat tagaagactt cgttgctcgg
    16621 aaccttgttc gcgcctctac cacaaaacga gcgcaaagcc gcagaaagac attagaaaaa
    16681 atggaccggc tggatcgtcc tcaaggggat gaaaaacaag caaactttct attcagtata
    16741 gaaaagcctt ctggaaatgt agtcttgcaa atcgaagatg ccgctatcgg atatgataat
    16801 gtcaccttat ctgaaccaat caatctagat atccgtcgtc aagaagcgat tgctctagta
    16861 ggtccgaatg gtatcgggaa atcgacgttg ctgaaatcaa tcattcacca gatccctttt
    16921 atccgtggaa aagaaacgct agggacaaat gtcaccatcg gctattacga tcaaggtcaa
    16981 gctgatctgc atacaaacaa gacgatccta gcagaattgt gggatgagca tcctaccact
    17041 ccagaaaaag agatccgaag tgctttaggt gctttcttat tttctggcga agatgtagaa
    17101 aaaccaatcc ctctgctaag tggcggtgaa aaagcacggg ttgctttagc caagttagcg
    17161 atgaacaaag aaaatttttt gattttggat gaaccaacaa accatttaga tattgacaac
    17221 aaagaagtat tagaaaatgc attgatcgat tatgacggca cactgctttt tgtttcccat
    17281 gaccgatatt tcatcaatcg gatcgcgaca aaagtcgtcg aactctctga aaatggcagc
    17341 aaagtctatc taggagacta tgattattat ttagagaaaa agcaggaaga agaagaaatc
    17401 gccgaattgt tggcacaaga tgagccagaa tctgctcctg ctcccacgaa caaatctgat
    17461 tattaccaaa gcaaagaaca gcaaaagctt atccgtagcc ttcaacgaaa aattactcag
    17521 gttgaagaag aaatggcgcg gatcgatgaa tggatcgatc agttggaaca agaaatggtc
    17581 gcgccagaaa acctggaaga tcacgtaaaa ctcaatgagc tgaaccaaga tttagaagct
    17641 gctagacaag atcaagaaac aaaattgacg gaatgggaag aactaagctt ggaattagaa
    17701 gaatatgaaa attaacgaaa aaaccaaaac agaaaccatt catgctgttt tggtttttct
    17761 ttttttattt tatcaaacaa aaattccata tttacctttt tcaccaataa gaagaccaat
    17821 tactagtcat tctcactaaa gcttctaaat aatagtaatc accccaaata caaggtgtat
    17881 ttaccccatt atttttgggc ttattataaa cagactgtgt taaaaagcca tcttcttctt
    17941 ccgttgtagc atatctttct ttcaattgtc gcataatttt ccaaacagca gactggtata
    18001 agttcttttc tgttttgtct aatagacctg attctattaa ttctaataat ccacaaacca
    18061 tgatcaccat actagatgta tctttttctt ctctgctaga atcatcaaag atcaaatccc
    18121 aataacaaat ggtatcttct ggcaaccgat tcataaaata attggctaat attttgctta
    18181 aatcaaaata tttttgttct ttcaaataac gataacttaa tgcaaaaccg taaatccccc
    18241 atgcttggcc tctagcccaa caggaattat cactataacc ttgatgcgta cttccatatc
    18301 ttggttttcc atctttgaca tccatataaa atgtatgata agttgaacca tcttctctta
    18361 ctaaataatc tgctgcattt tgaatatgag cctgagcagc ttttttatat ttttgatttc
    18421 cagtttcttc actcgcccaa tacaataatg gaagattcat gcaacaatcc atgatcattc
    18481 taccttgttg tgaaggatca ctaggatctc cccaagcttg tataactcct gccttctccc
    18541 aaaaacgctg aagtaatgtt tctgcagcat caattgaagc ttccttcatt tttcgatttc
    18601 ccgttatttt ataatctgct acggttgata aagaatacaa aaaaccaaga tcgtgatgat
    18661 tagtgataat ttgacagtca atcctgtgga taaaatctat atctgctttt tcagctgttt
    18721 gttgaaataa aggatcaccc gtcttctcaa ataacaacca attaattcct gtccaaaatc
    18781 cttctgtcca accaatattg tctcttaatg gataacgtcc ttttatagga cactcgcttg
    18841 ggaattgata ctgactgtta ctgatacgtt tgattaacct ctccgtgtgt ctgcgacaac
    18901 gctcgatctc attattccat tctttttcca ttaattcttt ataacattca tttatccatt
    18961 tttctggatg tattatctct tcaaacatca ctttatcaag catttcccca ccagccttta
    19021 gcataatttt agtctttttt tattgttttt attttggata gttagcccgg ttgtcccatc
    19081 aaaatcaatt ttgggacgat tgttccatac ttctaaacta taatcccgat cttgatgagc
    19141 aaccactcct gttccgagcc aatgggttcc ttttccaaat gtaccctcta atccaggaat
    19201 aaaagtgttt tcccatgtca tcaagtttgt attgggcaaa cccataatag ctgtggattt
    19261 tcgtttaata gtatcgtcac ttaaacagat aatacctgaa aagccagctt cactcaccct
    19321 atagttttcc ttgtcttctt ctatgattat tccttctttc tcaggagaac attcaatagc
    19381 aaaccctgtc tcatatagaa catattctgt atctaattct actttgtgta cgcgaatctg
    19441 ccagccttca aaaggaataa gccaagttct gatttttgcc tgtaaagtag gttcccatgt
    19501 agaggcaagt acctctttct tacatacaat gtctcgtaca ttatttcgaa cgatgaactg
    19561 tccataggtt ttgatgctct cattcttaaa actggtcgtg atatatgtgt ccgctcgctg
    19621 aatccctagc attgaatcca ttgccatttc tgctttcaat tgattcgctc gcggtatcga
    19681 aaaaccaaat tgactagaat aagcaaattt taaatatttt tcattggtca atgtatggaa
    19741 atttggccct aattgtcccg cattcaaaaa aaatgtatgt ccattatcat gttgtatgag
    19801 caaattggct tctgaaagaa gtttagttcc ttcacgaata ggcataggca tttctttagc
    19861 cgtccaatac tcatggtctt caggtagcgc caacaataaa aaaatcttat tcagccataa
    19921 tggagataac gtactattat agggttcagt caatgtcaat tgagggtagg tataaccaat
    19981 attcaggata cctttttcat caaatatttc tttttgcatc caccatctta tattccgatt
    20041 gactaacccc ttgatttcac ctaatttaaa aggcaaaaga tcggcccaca acatagctga
    20101 ccaaaagctt gaaactgcaa aacgataaat catgcttcga ccaaaaggca catttgcacc
    20161 atcttccgca aaaaaatgaa tatagtctct agaaaaatca atagctcggt tcttatatat
    20221 ctggctaatg ggcttttctg gttctagtac gctataaatc aaaccataat aatgaaatgc
    20281 aaaaggatta taataatctt ggcgacctct tgaactgtct cgataccagc cattttgaag
    20341 ataacaattt tctatattat caaatgcttc tttcagtgct gattctttaa ctggataacc
    20401 aaatttttgg ataattgttc cgacaattat cttaaaaaat tgccaattac catctgcaca
    20461 ttcatattca aaaatttgcg agaaccaatg aataatctgg cttttttctc ctgtatctaa
    20521 ttttttccat aataattgtt catgatgata taacgctaat aacattgcgg gcatttctac
    20581 catacgctgg tctctttcat gtatttctcc ccaataatcg ggatgggaag gatctgttcc
    20641 agcttttatt cctgatagaa ttaatttcaa ccatcgttca tctaattggt caattgcggg
    20701 ccccgctccc cataaaaccc gagagaatcc ttccattcct gcaattttat ttccatatcc
    20761 taccccttga tgtatataat caatttgtgc gccacttgct cgataaaacg gctggagtgg
    20821 ttgatttatc tttttaaata agtcaaagta atcctgtctg gtccttaata gtggtactgg
    20881 atgtttcatt gtattttctc ctctttttac taccaaatag ctaattccga ctgttctaat
    20941 cgaagtaagc cttcaataaa aaaataatca gaatagatca gtggcacatt ataagtcagt
    21001 tctctatggt atgcttctgc tccgcctcca atgatcccat cttcatccat attccaattt
    21061 gcatagtgct tttctgtctt ttggagtata ttgatagcag cttcatgata catttttgat
    21121 cgtacagagg aaacatatcc tgctaattcc agaagaccgc atgcagcgca tactccagca
    21181 ctggtatcaa tattcgaatc ttttacagca aaatcaatga gaggaatata accagttttt
    21241 tcgattttct gaataaaatt tactgctatc ttttctgcta tagctagata gtacggattt
    21301 tttgtgtaac gataacttaa cgcgaatcca tataaagccc atgcatttcc tctgctccaa
    21361 caagaattct cattgtatcc ttgcccgcca agtaaccgaa tatatttccc tgattctgca
    21421 tcaaattctg cgatatgccc ggttgatcca tctttcctca ctagatactt agctgttgta
    21481 tctgcatgct gccgagcaat tttagagaac ctcggatcac cagtttccct cgaagcccaa
    21541 tacagtaatg gtaaattcat catactatca ataatcatcc aaccaggttt atggtcattc
    21601 catgctacta aatagttccc tgaaatatta tagcgtccag caagtaccgt agctgcatgt
    21661 aaccctagtt gtcgactatg tttgttttga gactgttgat aatgtgcaat agccgatggt
    21721 aagaataaaa aacctacatc atgatgaacg tctgaaaaat ggtacagggc agattctaat
    21781 aattgttctt gttgtattgc tgcctctttg aacttttctc gttttgtctt gtgaaagagc
    21841 tgccataaaa taccactcca aaaaccattg gtccaccagt cgattcctct gtcctttatt
    21901 acgtctgtat attttccgtt gtaagaaata tatggaattt tattccccat tctgtctaat
    21961 tccgcatcta tcttttcaga agctacattc aaaatttttc ttgaccattc cttcgtctgt
    22021 tcattcatgg atatcccgcc attcttttaa tttttatgaa tgtggagttg ctcctagaac
    22081 gaactagatg gttaacattc gttatttttt ttacttattt gcagctccaa tgtcattctc
    22141 cttaaattta gactgcgccc acacataaat caagaccatt gggatcactg aagttgctaa
    22201 aattagcata attgcgccat aattaattgg ctcacctgta ctagaaaccg tcatattttt
    22261 gataattaag ggaaccgtat atttatcttt atctgaaagc atgactaatg gaaatagata
    22321 ttgattccaa cttcccataa agataagtaa accactagta ataatcgtag gtgataaaat
    22381 cggtacagcg attttcgtaa aaatcaccca ctcattggca ccatcgattc tggcagcctc
    22441 catcagtgct gtaggaaatt gactgctata ttgtcgcatc ataaacactg aagttgtact
    22501 ggcaatcgaa gggatgatca cacctgcata agtattcgat aatcctaaac gactcataat
    22561 gtaaaataaa ggaatcaatg tggtaaaaaa aggcaccatc ctagatagca taactgtatt
    22621 gaacaacagt ttattcccca taaaacgata ctttgctaat gcataacccg ccattgtaga
    22681 actggctgta gctgaaactg tactgattac agcaataaca atcgaattcc aaataatcaa
    22741 gggataatta tatctcgcca tgatggcttg ccagtttttt aatagcatat ccccaaactt
    22801 gaaattgatt ccgccactta aaatttcttg attactttgc gttgaagcta caatcatata
    22861 taaatatggc atcaaaaata ttaaggcacc aaacacaata atgacgtacg taaaaattct
    22921 gctttgtatc ttttgtttag tcatttttat cacctccgaa tttaaattga atagccgata
    22981 atataacaga tacaaacacc atcgtccagg caatagcaga ggcttttcca aaatcaacca
    23041 tttcaaaact tgttttatat aaattgactc ctacgaccaa agctgaattt tctggtccat
    23101 tggaagtccc aaagaatata tttgggatat tgaaattacc caagccattg atagtggcta
    23161 aaactgtggc cattaaaatg attggcttta gcatggggat cgttaataaa aagaattgcc
    23221 gaaatttcga tgctccatct atttcagcac tttcgtatat ctctttagaa atcccttgaa
    23281 gaccagctaa aaataaaata gtgttatatc ctacactaag ccaaatcatc gtgatgatca
    23341 ttgatattcg tatccaaaaa ggatttgtta accactgtac accgttaatt cctagcgcat
    23401 tcaaaaatcc attgaataac ccagtcggat tgaaaaaagt catgaatacg gaagctacag
    23461 ccaccgttga tgtaatggtt ggaatataat agacagttct aaaaaatcct tttcctttta
    23521 actgtgctga attcaataaa acagcaaagt aaatagctaa tagcatcgtt attggaattg
    23581 atcctgccat gataatcaaa acattcgtgt aagatttaat aaacaatgga tctcggaaga
    23641 tagctagata attgtcaaat ccaacaaatt caaatgagta gccaattttc ataaaactaa
    23701 aagcaaacga gataatgatg ggaatcaaaa tcgtcacaat aaagaaaata acaaaaggtg
    23761 tgaccaatcc ataagcactt tttttatgag acgtatctgt tgcttttttc ttgctgttct
    23821 ttattgtctt gcttggtaac gaagattgca tcatcgttaa gtcctcctaa tattttttaa
    23881 tcaccagtta attttacgcc gtacttatga gcaaaatttt ctgcttcttt tagatatgaa
    23941 ttttcaaaat cacctgtttg ccaatagtta tatgcagcgg tagtcaaata agacgtagca
    24001 tctgaataag tggctggtaa aacagtattt gatgtatttt cattgatttt attcaatagt
    24061 tcatagtatt tttgtcctcc ttagtagtca aactttttct cagctgcttc tgtttgatag
    24121 gccaatgagt tagatgtagc cacgcccagc tccagtgcgt tggctaagca ttcttcatct
    24181 gtcagcataa atgtaagaaa ttgttgtgcc acttgtggat ttttggcatc tttagggaca
    24241 taaaaacttg atcctcctga tactgcacct tttcctggat tttcttttga aaaaggaatc
    24301 agtggagcca tgcgccattt gtcagatgca tcaggaaaat tcaaagacat attcgtagca
    24361 taccagccgc cagcaacaaa cattgcagat tcttgaaatg ctttctcagc atcttgtgca
    24421 ccgtagaaat gtacaatatc tgagtcgatc atttctttga ttaactttgc agcatgtttc
    24481 gcttctttag tattcagacg gatatttcct tcttcatcaa gcaaagattg attctgctgg
    24541 gctagcatgt tgataaacaa ttcttggtcc ccagattctg gcaaggcaat tccaaaacta
    24601 ccagtttttt cttttaatgc ttttgccacc tggatatatt cttcccaaga actgatttct
    24661 tcaaaatcta cacctatttc agcaaatttt tttggttgat aataacaaag tgctggggta
    24721 aaatcactag cccaaggaat aatcgtttca ttactcgttt gttgacgcaa aatattcaat
    24781 ttttgatcat aaaactgatc gccatatttg tcataaaatc catattcatc agcactataa
    24841 aaagcatctt gaaatttttc cagcacacct gctgctgaag tgtctgctat ggagataata
    24901 tccggcatat cttcgtgtgt taccaatttt ggggtgatca tttgtactgt tgctgttccc
    24961 gaagctacat tcacaatttt taagtcaata tttgctccag ttttgcggtt atagattttt
    25021 gcagcatttt caacatagtt tgtcagtttg ccgtttgcga catatacttc taatgtagtg
    25081 gcttttttta tcattggatc tgcgatgttt gctgttactt cttcgttagt ttttccacat
    25141 gcacttaagc tgaataatag ggtaatgttt agcaaagcta tcaccaattt gtatcttttt
    25201 ttcatgtcta tcccttcttt cttttcaagt tcttatataa aacgctttca cttatgtata
    25261 ttaacggaat tagtaacaca aatatttata taaaaaaata ttttacttgt cgttttggct
    25321 tttca
//
LOCUS       DACEPD010000039        24038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.039, whole genome shotgun sequence.
ACCESSION   DACEPD010000039 DACEPD010000000
VERSION     DACEPD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.039"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(157..744)
                     /locus_tag="IW797_001460"
     CDS             complement(157..744)
                     /locus_tag="IW797_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224243.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            919..2241
                     /locus_tag="IW797_001461"
     CDS             919..2241
                     /locus_tag="IW797_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1224244.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            2396..3172
                     /locus_tag="IW797_001462"
     CDS             2396..3172
                     /locus_tag="IW797_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ1224245.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            3165..3734
                     /gene="rnmV"
                     /locus_tag="IW797_001463"
     CDS             3165..3734
                     /gene="rnmV"
                     /locus_tag="IW797_001463"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ1224246.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            3727..4611
                     /gene="rsmA"
                     /locus_tag="IW797_001464"
     CDS             3727..4611
                     /gene="rsmA"
                     /locus_tag="IW797_001464"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ1224247.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            5062..5691
                     /locus_tag="IW797_001465"
     CDS             5062..5691
                     /locus_tag="IW797_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224248.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(5815..6924)
                     /gene="ugpC"
                     /locus_tag="IW797_001466"
     CDS             complement(5815..6924)
                     /gene="ugpC"
                     /locus_tag="IW797_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ1224249.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            7133..7555
                     /gene="mgsA"
                     /locus_tag="IW797_001467"
     CDS             7133..7555
                     /gene="mgsA"
                     /locus_tag="IW797_001467"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ1224250.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            7878..8414
                     /locus_tag="IW797_001468"
     CDS             7878..8414
                     /locus_tag="IW797_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1224251.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(8454..10238)
                     /locus_tag="IW797_001469"
     CDS             complement(8454..10238)
                     /locus_tag="IW797_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224252.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     EEKPDLPDAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGA
                     GKSTLVNLLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLR
                     FGNPEASDAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMIS
                     NPPMLILDEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGA
                     IVEMGTHDELLQKDGYYRRLYQAQFTAQ"
     gene            complement(10241..11956)
                     /locus_tag="IW797_001470"
     CDS             complement(10241..11956)
                     /locus_tag="IW797_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224253.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(12735..12959)
                     /locus_tag="IW797_001471"
     CDS             complement(12735..12959)
                     /locus_tag="IW797_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224254.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            13159..15927
                     /locus_tag="IW797_001472"
     CDS             13159..15927
                     /locus_tag="IW797_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1224255.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            16008..16427
                     /locus_tag="IW797_001473"
     CDS             16008..16427
                     /locus_tag="IW797_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ1224256.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            16439..16909
                     /locus_tag="IW797_001474"
     CDS             16439..16909
                     /locus_tag="IW797_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1224257.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            16926..17705
                     /locus_tag="IW797_001475"
     CDS             16926..17705
                     /locus_tag="IW797_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1224258.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            17692..18513
                     /locus_tag="IW797_001476"
     CDS             17692..18513
                     /locus_tag="IW797_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1224259.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            18530..19579
                     /locus_tag="IW797_001477"
     CDS             18530..19579
                     /locus_tag="IW797_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1224260.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            19592..20596
                     /locus_tag="IW797_001478"
     CDS             19592..20596
                     /locus_tag="IW797_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1224261.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            21229..22248
                     /locus_tag="IW797_001479"
     CDS             21229..22248
                     /locus_tag="IW797_001479"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1224262.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            22291..23172
                     /gene="galU"
                     /locus_tag="IW797_001480"
     CDS             22291..23172
                     /gene="galU"
                     /locus_tag="IW797_001480"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1224263.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            23472..23849
                     /locus_tag="IW797_001481"
     CDS             23472..23849
                     /locus_tag="IW797_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224264.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT         7808 a         4158 c         4963 g         7109 t
ORIGIN      
        1 ttatatctaa cagtagcgtt gatgtgttaa ttataagttc tatcacttta tgttcaatta
       61 gtttgtgctc ttatatgtgt gattgaaaat atagataaaa aaacaatcat cggctttgac
      121 ttcgatgatt gttttcttta ttttgtaaaa atcctatcac ttgattttat ggtaaccttt
      181 tatttttgtc ttcaaacggc ttggtaagag agcagtaagc agtgcaaatg ggatcgaaaa
      241 gacataccag aaaatcgtac gtccgatacc ataatgttga tagagctttg tcaacatcac
      301 gtgtaacaag atcaacccta aaagcaagga gccagctata aaccaatgat tatcccaatg
      361 catgacttca ctaagtgcca tcactccaaa aggatagaaa taaaaataga tcccgactaa
      421 ggtcatccgc cagttctttg taaatgttcc gatattcaat aaaaagaaaa tcgctgaaag
      481 caaaataccg ataggcggaa aaagcccaac caaaaaggaa tacaacatcc cccacaagat
      541 catcacagga cctttgatca atgcaacgat tgcgatgaac aaagtcagca atattagttt
      601 ttgtgataac gttgcaacag tcattaatcc aaataaaatt aatgtgaacc aatgccaatg
      661 ggactgctgt tgttctttac gggaaaaatc gacccggttg gataaataat ctgctaattt
      721 cggataagaa cgcttaaaat ccataaactg ttcctccttc gtggaaaagg taggtttagt
      781 atacgaaaaa taaaaaagaa atacatccgt cttaagaggg agtttttcac tgaaagtgga
      841 gaaaaaagaa tggtaaatta aaatattctc agttatactg aaaattatac caaatttcaa
      901 agaagaaaga ggatgggaat gaaaggcaaa ctaaaacgag aaatcaatct gtttggcgca
      961 ttagctaccg ttatgggaac cgtgatcggt gcaggcgtct tcttcaaaac tgctgctgtt
     1021 actgctagta ctcagtctac aagtttgaca ttgctagctt ggcttttggg tggtttctta
     1081 acgatctgtg ctggattgac agtggctgaa ttagctacag caatcccgga aactggtgga
     1141 gctgtaaaat atattgaagc tgcgtatgga aaattgccca gctttttatt aggatgggcg
     1201 caaagcttga tttactttcc tgctaatatt gctgcattat cgattatctt tgcgacacaa
     1261 ttgacgaact tattacaatt atctaccgat tatcttttgt tgattgcagt gatcaccgct
     1321 gtttctgtga cagggttgaa tttgttaggg acaaaagtcg gtgcttctgt acaatcagtg
     1381 acgttgattg ttaaattaat accaattgct gtgattgtca tatggggatt attgacccca
     1441 ggacaaggaa ctgtccagtt atttccaatc gaagctggaa aagatgttac ttttgtcgaa
     1501 ggattgagca gtgcgttgtt agcaacgttg tttgcttatg acggctggtt aggtgtcggt
     1561 gcaatggcag gggaaatgaa acggccggaa aaagatttgc ctaaagcaat tattcttggt
     1621 ttgagttttg taacagtcgt ttatctattg atcaattttg tttttttgaa aactttgccg
     1681 attgatcatc ttgccggtaa tttgaatgca gcttctgaag catcagacgt catttttgga
     1741 ggaatcggtg gaaagctggt cactattggg atcttgatct cggtatacgg tgcattgaac
     1801 ggttatacac taactgggat ccgcgtgcct tatgcgatgg ctttagaaga cgagctacct
     1861 ttcagtaaac agctgacaaa gctttcaaag aagtttacgg ttccttatgt tcctgcggtt
     1921 ttccagttag cagtagcttg tattatgatg agccttggtt ctttcgactt tttaacagac
     1981 atgttgattt ttgtgatgtg gctattcagt ttactgattt ttatcggtgt ctttatttta
     2041 agaaaaaaag cgccggaatt gccacgtcca tataaagtac cactttatcc aatcgttccg
     2101 attattgcga ttctcggtgc tattttcatt ttaggtatga cgatgatgac gcaaacgaaa
     2161 ttagcattga tcggtatagg tgttaccttg ataggtatcc cagtctatta tcaaaaaaag
     2221 aaaaaacgct cagaagagta aaatatttca acaacagaca gaaaaagtac gaactaggtc
     2281 ctctagatct tcgtgctttt tctatttcaa taaaacaagt tcttctattt tgatttttgc
     2341 tacttctgca agtagtgctt atgtgctaaa gtaagaatga aaaggagaga taaacatgat
     2401 atttgattct catacccatt taaatgctga acagttcaat gaagatattc cagaaacgat
     2461 tcagcgagca caagaaatgg gggtaacgaa aatggcggtc gtgggcttcg atacgccaac
     2521 gattgaaaag tctttgcaat taagccatga ctatccaaat atatacagta ttatcggctg
     2581 gcatccgaca gaggctggca gttatacgaa agaaatcgaa aataaattac aagaacaatt
     2641 gactttacca aaggttgtag cattagggga aattggcttg gattatcatt ggatggaaga
     2701 cccgaaagaa gtccaagata aagtatttcg cagacagatt gctatcgcaa aagaaatgaa
     2761 cctaccaatc agtattcata ctagagaagc acttgaagac acgtatcaaa tcctaaaaga
     2821 agaagatgtt cgagatatcg gtggaatcat gcatagtttc agtggtgatt atgaatgggc
     2881 aaaacgattt cttgacttag ggatgcatat ttcatttagc ggtgtcgtca ctttcaaaaa
     2941 agcattagac gtccaagagg ctgcagcaaa tgtaccaatc gaacgattac ttgtggaaac
     3001 agatgcccct tatctggcac cagttccttt tagaggaaag cgaaatgaac caggctatac
     3061 tcgttataca gtagaaaaga ttgctgaact tcggaatctt ccgacagaag aagtggctgc
     3121 ccaaacatgg acaaatgcgc accgattatt tggtctaagc gaacatggat aagatgaaga
     3181 tccaagagat tatagtcgtt gaaggaaaag acgatacaag aaggctgcgg gaagtagttg
     3241 atgcagatac gatagaaaca atagggtctg caatcaatga agagatcctg atgcaaattg
     3301 aacatgccca agagactcgt ggagtgatcg tttttaccga tcctgatttt tcaggagaaa
     3361 aaataagaaa aatcattatg gaagctgttc ctcaggcaaa acatgctttt ttgccgcgca
     3421 cacaagctaa accaaagaaa aagggaagcc ttggtgtcga gcatgcaagt gatgctgcta
     3481 ttttagaagc attgaaaaag gtcgtgacgc cagacatttc ccaaacggct gtttctgaaa
     3541 tcactagaca agacctttta gactatggtt taatagctgg ggcacaagca aaagaaaaac
     3601 gagaaaaact aggtgacgag ttgcgcatcg gttacacgaa cggcaaacaa ttggaaaaac
     3661 gtttgaagat gtttcgtatt acgccgaaag aactcgccca agcaatgaaa atagtggagg
     3721 aacaacatga gtgaatatcg tgaaattgcg acaccctcta gaacaaaaga gattttattg
     3781 aaacatggtt tttcatttaa aaaaagttta ggacaaaatt ttctgactga accaaatatt
     3841 ttaaggaaaa tcgtagaaac tgcgcaaata gatgaacata cgaacgtgat cgaagttgga
     3901 ccaggaatcg gtgcattgac ggaacaatta gccaagcatg caaaacaagt cgtagcattt
     3961 gagatcgatg atcgtttgat ccctgtattg gctgatacga tgcaacccta tgacaatgtg
     4021 actattgtac atcaagatat tttaaagacc gatttgtcaa cagctgttag agaaacattt
     4081 catgaggagc ttccgttaaa agtcgtagcg aatttacctt actatattac gacaccgatc
     4141 atgatgcatt ttcttgaatc agatttggtt gtggatgaac tagtcgtcat gatgcaaaaa
     4201 gaagtagctg atcgtatctc tgctgaacca ggaacgaaag cttatggttc attatcgatt
     4261 gctgttcaat attatatgga agccagttta gcattcatcg taccaaaaac agtatttgtc
     4321 cctcagccaa atgtagattc agcgattttg aaactgacaa gaagagatat accggcagtc
     4381 gaagtgacag acgaaaaagc atttttccga ttgacaaaag cggcatttca gcaaagaaga
     4441 aaaacgcttt ggaataactt gcagcattcg tatggcaaag atgaccagac aaaagcatgg
     4501 ttagcaaaaa gcttggaaac agctggaatc gatccaaaac gtcgtgggga gactttgtca
     4561 ttacaagaat tcgccgcatt aagcaatgcg atgtcagaaa atatgcagta aaaaaagagg
     4621 ttgtgacaga agttgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa
     4681 atagttcctc atgtttttcg tttgtttggg cttattaccg cagatgcaag cttttgaaca
     4741 ccgtttaaga aagaggttgt gggcctgccg tttaactcta agtcacaagg aacaatttca
     4801 aaaaacagct tctcatgttt ttgagttagt tggtcttatt acttgatgca agcttttgac
     4861 cactattatg ccgaacaagg agatgtgaca caaattttgt cacatctcct atttttgttt
     4921 cttgatcaaa aaattcttat acttgatact cacactcatt ttgtatggat ttagaaaaga
     4981 atatgtgtct tgacagttat tagtaagtga gttatactaa gtcgacattt atgtgtcaag
     5041 acatattagg agagagaaaa aatgagaaaa aattcagttc gaaaactaac gatttctgct
     5101 ttattgattg ggatgggggt cattatccca atggtcatgc ctaaaatcat gattccacca
     5161 gcttccttta ctttggcgtc tcatgtgcca ttatttattg cgatgttttt tacaccagga
     5221 gttgcagtgg ctgttgcgct aggtacgaca tttggtttct ttttaacaac gccggttatc
     5281 atcgcattgc gagcattgtc acatcttgtg tttgcgttga ttggggcttg gtacttgcaa
     5341 aagcatccga gtatcgtttt aaaaaatgga cagtttactt ttgctaattg gcgtttccaa
     5401 gggttcaatt ttgtgatcgg tgtgattcat tctttagcag aaatgctagt agtcagcatc
     5461 ttttacttta ttggtaatat gcctgaaaca tattattctc aaggatattt ttatacagta
     5521 ttcatcttga tggggattgg tgggcttatc cacagtttag ttgattataa tattgcttat
     5581 tttatagcag gaacactcag caaatatttt gatatccccg tatttacggc agcaaaaaaa
     5641 gaaaatcgga aagagtcgat tcaagggtac gcgaagaaaa cgacatatta gacatgatct
     5701 ctcaaaaaaa aaactctcat ctagtaatgg ataataaatc ctctaagatg agagtttttt
     5761 gtcctaaaaa gagctgcacc aacatcttct ggcacagctc taacaggggg caatttatgg
     5821 aagtttgatg acttgttctg tttctttatc aaagaagtgg cctttgctga tgttgaaggc
     5881 aaggtcgact atttcgcctg gtttatggaa atctcttgca tctacttttg agatgaactc
     5941 agtagaacca gtttttgtgt aaagcatcgt ttcagctcca agtaattcgg aaacgacaac
     6001 ttctgatttc actacggctt caggtgaagc ttcaagggct acttgttcac tgtggatgtc
     6061 ttctggacga ataccgaaga taacttcttt tccttcataa cctttttcaa ctaatacttt
     6121 gttttttcct tcaggaatac gaagcttaag cccatgtcca tctgtgataa caccattttt
     6181 caatgtcaca ttaaagaagt tcatggcagg agatccaata aagccggcaa cgaacatatt
     6241 gacgggtgta tcataaactt ctttcggtgt accgatttgt tggataaatc cgtctttcat
     6301 gataacgatt cggtcagcca tagtcattgc ttctgtctga tcgtgagtaa catagatggt
     6361 cgttgtttca agacgctggt gaagtttggc aatctcagca cgcatggcta cacgtaattt
     6421 agcatctaaa tttgataatg gctcatccat taagaagact tttgcatccc gaacgattgc
     6481 ccgtcccaat gccacacgtt ggcgttgtcc accagataaa gcagctggtt ttcgtttcaa
     6541 gtattctgtc agacctaaga tctctgctgc attgtccaca cgttttttga tgtctgcttt
     6601 atcatattta cgtaatttca agccaaaagc catattatca aaaacagtca tatgaggata
     6661 caatgcgtag ttttggaaaa ccatggcaat atcacggtct ttaggtgcca cgtcattcat
     6721 gacttttccg ccgatattta gttctccttc agagatatct tccagtcccg cgatcattcg
     6781 caaagtagtt gactttccac aaccggaagg accaacaaaa acgataaatt cacggtcttt
     6841 gattgataga ttaaaatcag ttacagaata attttctgca ttgtcatatt ttttatatac
     6901 gtttttcaga gccatttcta ccataatacg ttccacactt tcttttttag ttttattaac
     6961 tatttaaagt ataaatgaaa gcgttaacta acttcaatgt catgttgaac aagaattttt
     7021 tctttttttt gtcaatgtgc tagttttaag ctttaataaa tatccataag aaataaaaac
     7081 tgatacactg gagtaaataa gtatgttaac aatatcaaaa ggagtgtctc ttatgaaaat
     7141 cgctttgatc gctcatgacc gaaaaaaacc tatgatggtg aaattagcaa cagcgtataa
     7201 aacaatattg gaaaaacatg aactttttgc taccgggacg actgggcaga ggatcacgga
     7261 agcaaccggc ttgccgatcc atcggtttaa atccggaccg ttaggcgggg atcagcaaat
     7321 tggagccatg atttcggagg atcagttaga tttggtgatt tttcttcgag acccgctggc
     7381 tgcacagccc catgaaccag atgtaacagc acttatccgt ttatctgatg tctatgagat
     7441 tccgttagct acgaatatcg gaacagctga ggtgctacta agaggactgg aagccggctt
     7501 cctagatttt cgaagaatcg tccatgaaat agacaagcat ccactttcat tttgatggat
     7561 aagcgtatgg aaaaataacg tttaatgttc gcctatatgt agctataaaa gctaaacaaa
     7621 ctgataatat gcgatataat acgaacgtat tggataatat aaacaataat attagttttt
     7681 cgtatttgca gtaaatgtga aaatatgcta tagtttctgt gggaaaagat cccaaaaata
     7741 atacagagta gaaaataaag cgttaagtgc taagaggatg ggatgttgcc tcttggacga
     7801 aggacgtaat gttcgcggtt ttattttcgc attcgctgca tgatacgatg tagcagctct
     7861 taaaggagat gctaaaaatg aagaaacacc ggttagacgc acgtatgatt gctattatgg
     7921 gcttattgat tgcattgatg gtcactttgt cccgactggt agcaatcgag accccattta
     7981 ttaaaatcag tgtcacattt attccacaag tgatcatggg cattcttttt ggtccttttt
     8041 ggtcagggat aggcgcagtt ttagctgatt tagtaggaat ggccctcttc tcgaaatctg
     8101 cattctttat tggatttaca ttgaatgcat ttattgaagg agcaatctac ggattctttt
     8161 tctatcgaaa agagatcact tggaaaaatg ccattctggc tacgcttagt gtcacgctga
     8221 tcatcaactt atttttgaca ccattatggc ttgcgttgat gtaccatgtg ccattgttca
     8281 gctgggtcgt ttgggcgccc cgcctgttaa aaacagtaat atggttaccg attcaatcaa
     8341 ttgccattta ttacgtagga cgtagtattc cttacaaaaa aattttaaga agtttagcga
     8401 tccacgctaa ataaaaaaca agcagtcttc ccatcccaga agactgcttg tttttattgt
     8461 gctgtgaatt gtgcttgata gagccgtcgg taataaccat ctttttgaag cagctcatca
     8521 tgggtgccca tttcgacaat agctccctga tccatcacta atatctggtc cgcatttttg
     8581 attgtggaca ggcgatgggc aatcacaaaa ctggtttttc cttccatcat tgctaagaag
     8641 gcttgttgga tttttttctc tgtcaatgta tcgactgagc tggtagcttc atctaagatc
     8701 aacatagggg gattactgat catcgttcga gcaatcgtca acagttggcg ttgtccatct
     8761 gatatcttta acccttgacc gccaatctta gtatctaatt tttccggcaa acgttctaca
     8821 aattcataca tataagaggc ttttagggca tcattgattt ctgcgtcact tgcttccgga
     8881 ttcccaaaac gaagattttc tcgcaatgta ctgtcaaata gccaagtatc ttgtaacacc
     8941 attccaaaac ttttacgtaa tgtatcacgt tggatagagc ggatgtcatg gtcatcgact
     9001 gtaattgttc cttttgttgt gtcataaaaa cgcatcagca aattaacaag tgtggatttt
     9061 ccggcccctg tttttcctac tatagcaatg gtttgacctg gataagcagt aaaattgaaa
     9121 tcttcgatca aacgctgctt ggggtcataa gaaaaagaaa catgatcaaa agagactttt
     9181 cctttgatat ttttgggttc caaggcatag gcatctggca aatcaggctt ttcttctggc
     9241 aaatcaggct tttcttctgg ctgatcgatc aaatcaaatg ctcgttctag accagaaaaa
     9301 gcggtttgca gttgtgtcgt gattccagaa agttcgataa atggtttaga aaactgactt
     9361 gagtagatcg taaagctgga aatcacacca acagtgattg acgaaccact cttcaatgcc
     9421 aataggccgc caactaaacc aatcgataaa taagcaaggt gatctacaaa acgagaagac
     9481 gggtttgtca aggaagaaga aaattgtgcc cgttgtcctt tttcatatag atcagcattt
     9541 agcagttcaa attgctgttg actagcttct tcttgctgaa aagctttcac gattttctgg
     9601 ttaccgattc gttcggtcac aaagcccgag atatccccaa taatcctttg ctgtctatcg
     9661 aaatttgttt gtgaagcacg agcaacgatc caactcacaa ggaaaataat tggtgtagaa
     9721 caaagaacga ccaatgtcag aagtggactt agacgcatca taaaaataaa cgaaagaatg
     9781 atgattgcca ctccagaaaa taattgattg aaggccgcag aaattgcaat ggaaatattg
     9841 tccatatcat ttgtaaatcg actgacgata tttccgtggg agttttgatc ataataacga
     9901 agtggcaatc gattcagatg agcaaatgca tcttttctta attttgcaac ggataagtaa
     9961 gctaaccgat tgcttaacac ttggatgagc cattgactga ctacgttgat taaaacgata
    10021 ccaccgaata gaataagtaa atggaacagt cgattgaagc ggacctgtcc ttctccaatc
    10081 atttgatcga tactttgacc aatttgtaac gtcatcacaa ctgttgcaat cccattgatg
    10141 attcctagta tcacagctag ccaaatgtct tttgcatagg cagtaaggta aggaatgaat
    10201 cggcggagcg tctgcaagga taattttttt actttcataa ctattcttcc tcctgtgatg
    10261 cgacgatttc ctgatattct gctgattgag caagtaattc ttcatgggta ccctgagcag
    10321 caatcttacc ttgatccatc actaagatat gatgtgcttc ttgaatagag cggacacgtt
    10381 gggagataat aatcactgta ctgccaagat aagtctgtag cgccttacgc aaatccaagt
    10441 ctgtttgata atccaacgca cttaaagagt catctaaaat cagtaatgct ggcttggcaa
    10501 tcaaagctct tgcaatcgtc agtcgctgtt tttgtccgcc agagaaattt ttcccatttt
    10561 ccatgacttt tgtatctaaa ccatttggaa gctgacggac aaattcttct gcctgagcga
    10621 ttttcagagc ttcccaacat tctttttctg tggcgtcttt cttaccccat tgaaggtttt
    10681 cacgaatagt cccggtaaat agtacagaag tttggggaac aagagcaatt tgctgacgga
    10741 gatgatccag ctgccaagaa cgaacatcca ttccgtccat tataactgta ccggaactga
    10801 catcatagaa acgcggaatc aattgtgtca aagtcgtttt cccgcttccg gttggaccaa
    10861 tgattccaag gactgtattt cgcttcagct gaaatgtaat attttggaga gaaagaccag
    10921 aatcaggagt ataacggaag tcaacatggt caaaagcgat aattgcttca gaagaaaaat
    10981 cgggagtagt actgacctca gcttcaagaa tcgaattttc tgtgtctagc acctctttca
    11041 cccgatttcc tgaagcttcg gcacgtgtga agatcacgac gaggtttgaa acaacgatca
    11101 gtgccaaaag catttgattc atataattga tcaaagcaag aacttgtcct tgttgcaagc
    11161 taccaatatt tactttgatc ccgccaactg tcagcaaaca gatgatcccg acattcatga
    11221 tcaaggttgt agcaggtgat aatagggcag agatattgga aacacgtaaa taattgttcg
    11281 ctagatcatt tgtgctttta tcgaacttct ctgtttcttt ttttgtctgc gcaaatgcgc
    11341 ggatgacacg aacaccgcta agattctgac taacgtgttc attgagctga tctagttttt
    11401 cttgcacttt ttggtagaga ggaacggttt ttttgatgat aaaaaataaa atgattgaga
    11461 aaataggcaa taatgctaag aaaaacagac ctacttccca gtcgatcaca aaagccatga
    11521 ccactgaccc gatacttaaa aacggtgctc tgatcaataa acgaatgacc attgctaaag
    11581 ctagctgaag ctgattgatg tcgtttgtca ttcgggtgat caaggtatct gttccaaaat
    11641 gattcaactc gttgtgagag aaagtattga tttttttgat tagctgatta cgtaactctg
    11701 taccaaatcc ttgtgaagct actgaagcat agtattggca aatgatagca caaatcagac
    11761 caatcacaga catagcgacc atccataagc ccatttcaat gatttttcct cggtcattca
    11821 tttttagtcc attatcgatc aaggaagcca tcattaaagg caggacaagt tcgaagacag
    11881 cttcaagaaa tttgaagaac gggcccaaaa tgatttgctt acgatagtct tttgcataac
    11941 gtaataactg aatcatatga ttcctcctat tatttatttg tccacaattg aaaaaacaag
    12001 tactaccaca ttgtagctta ggaaatttag aatgaaaaga gaacaagcct tttcataaat
    12061 gaaagtttta ttatgaagaa gctttagaaa ttgaatagac tatttttttt aaaaaaaaga
    12121 aaatgagaaa acatgccagt ctcttcattc ataggaacgt ctattttact taatttttct
    12181 ataaatgaat tctgcataaa aaatatatca agtaaatgtt tatagatgaa actctattta
    12241 cttctttttt aatttgttct tgcaatcttt tttgctacgt aatttttgta aaaaagtggg
    12301 ttgagagaaa tctaattgat tttcaaagaa ggagtatgaa aatgagaaaa atatttattc
    12361 gtttattgtg ggtactatct tagaggaata aataatagtg gagaaaacta tggtttggcg
    12421 cagattctat caggtttgat ggaacgaaga caacctcccg acatcaagtc aaaagctact
    12481 tgagattttg atctgaaaat aaaaaaagaa aacaaggtag gaacatcaat gtttctacct
    12541 tgttttcgta atcaatggga aaaaactaag agtggtgtac gtaatcaata aaaaggatgg
    12601 tgacaacagt acctctttac tctctgaaag catcataact cgtttttcct cattatgcca
    12661 ataaattgtt gttataccac atataaggat ttgtaatagc taaaaacaga caggaaagtt
    12721 gaaatctgtg aatcttattt tgtatggaag gatttataga acgcaaaaat caagataaag
    12781 atgaataaaa atcctgtttc ggctatatat tgcgtatcta cgccaataaa acgagtttgt
    12841 tctgtcatag ccatataaag caaaggcaaa aatacaacta cagcgagata agatccgatg
    12901 atttctcgaa aagttttcca ggaagtatgt ttcctgtttt tatatttatc cgatttcatt
    12961 gaatagtcat agcctcctta ttctttcata tgttgaaatt ccagtataaa aactggaagg
    13021 aactgtacgt tttttagaat aaaaattata agggataagt aggctgctgt ctataataag
    13081 atatattttt caattcttta ttaaagattt tattgacaaa atgaatccgt tttctatgat
    13141 ggaggagggg tgaataagtt gttgaagaag gaaattttat gttacttaga gaaccagaca
    13201 gcctttttcg atttagaaca tatgaacgag gtctttacag cacaaaggtt agctgatcat
    13261 tttggggtaa aaagaaacac agtcagtcat tatttgaacc agttaaatga tgagcaacta
    13321 ctagtcaaaa tcaattctcg accagtgatt tatttccaca aagaagcttt tgaaaaacaa
    13381 ttttttaagt tacgcacgaa tttttatttt tctatcgatg atctcaaaga ggaacagcct
    13441 tttttcgcgc aaccaaaaga cttattctct tttatgatcg gatacgacgc cagtttgaaa
    13501 gaaagtatcg aacaaatcaa aacagctctt tactatccag atggcggatt accacttttg
    13561 attacaggcg aaagtggcac aggtaaaagt tatttagtta atttggtcta tcagtattgt
    13621 ctgcttcatg atctgctaga agattctgca ccattcatca cttttaattg tgcacaatat
    13681 gcagataatc ctgaactttt gactagcaat ttgtttggac atgttaaggg agcgtacact
    13741 ggtgcagaag aaaataaaaa aggggctttt gaagcagcag atggtgggat cttgttttta
    13801 gacgaggtac atcgcttaac accagaaggt caggaaaaac tgttcaccta tcttgatcaa
    13861 ggtgtgattt atcgtatggg agaacctaat ttgtcacgcc ggatcaaaac acgtctcttt
    13921 tttgctacga ctgaagagat tactagtcat ttcttaacaa ctttcattcg aagaatacct
    13981 atccgaatcg aactacctgc acttaatcag cgtagtcgta atgaacggct agaactgatt
    14041 tactcttttt ttctcgaaga acaaagaaaa atggggagcc ctttgtcagt cagtggacca
    14101 gctttgtcac ttttaacaag tcagaaatta tccggaaaca taggagagct gaaaaatatc
    14161 gtcaaagttt cagcagcaaa agcatatgct gaacagcgtg aacagcctga aatccatgtc
    14221 acgatccatc atttgcaaaa agaactactt gcgcagccta ttactaaaaa tacagcacag
    14281 caaggaatct atattacgca agaccagacc ttagagcaat taatggagaa gcaacagccc
    14341 gaacagcagc gaatcgttaa aagttttgag caaatcctaa ttgatttcaa gaaaaataac
    14401 tgtctgctat attcttgcga aggaaaatta aagcaagaag tgattcaatt atttgatttt
    14461 ttgctgtttg agaccagtag acaagaaaaa cacgagctgc tggtttattg gacacaatct
    14521 atccgagaga cctttcgaca aatggaaact gcctatcaaa tcaaattcga tggaaacagt
    14581 gtctatgcct tgagttacta tctctatcag cgaagcacta tcaaatggtt accggaagaa
    14641 catgatatta tccaactaat caaagaatta gaacaacaag tttctcagtc ttatccatct
    14701 agttatcact atgtccaacg aattctggaa ttaagtaaga tcaaattaga tattgaagac
    14761 acaagtatgg atcggatttt actgacgatc tatctaaaaa aagcgaaatg gtcaaaagaa
    14821 aaacgactgc caaaagcgat gattgctgct cacggttatg caactgctag cagtatggca
    14881 aatgtagtaa atcgtttatt acaagaggat ctatttgaat catttgatat gccactagat
    14941 gtcacaccac agcagattgc ttcagaaatc cttgattatt gcgaaaacag cgatgtttca
    15001 aatggattga tcatattagt agacatggga tctctgaaag aaatccatca gttttttaaa
    15061 aaacagttat ccgtaccgct tttgattttg aataatgtga cgacaccttt agcaatcaca
    15121 gtaggggaat gcctgcaaaa aaatgtatca ttagaagaga tagcagaaga gactgttcga
    15181 cagatccagc cagaatggcg actgctctat ccagaggaaa acaaacccaa agcactcatt
    15241 actacttgct tcactggaat cggtacggcg gtccacttaa gtgaactgct agaaaaaagc
    15301 ttacctacgg cttgtcagtt gaaaatcatc ccatatgaat atcagcaatt aaaagataag
    15361 aaaaacagtg acccattatt ttccatatac gaagtcgtag gaatgattgg caccacagat
    15421 cctgatatca ccaatatcga ctatctttca ttagaagacc tcatctcggg ggaaaagatg
    15481 tctgtactag ctgaatggtt ggacactact atgaatgctt cagagaagga aatattcaat
    15541 cagcgcatca ttcgtaattt ttcattggaa aaagtactgg attccgtgac tattctagat
    15601 acagagaagg tgatgggaga aatcgatcat tttatgagag aattagaggt gcagttgaat
    15661 cgaagcatca gcaatcccaa aaaattagcg ttgtatgtcc atgtgagctg tctcattgaa
    15721 cgattgatcc gacaaatgcc aatcgaaacc tatcaaggat tagagaaatg gaagcagtgt
    15781 caaaaagaag ggttatctgc tattaaatct gcgtttagtg tcattgaaga gcattatagt
    15841 gtcatgatcc ccaattctga gattgcctat atctatgata ttttgagtga agatacagag
    15901 tttctctcga tagaagaaga attttgaaca taagcaagtt gttgccacta attttggcat
    15961 gcaacttgct tttttagtat gtgtaagtga aaagaggaga tgaaaaagtg gatagagaaa
    16021 ttatcttagc ttcccatgga aaatttgctt cagggatatt agattcatta gagctgatct
    16081 atgggaaaaa tcattctatt actgtattcg attgctatac tgatgagaat tttgatttag
    16141 cggaaacagt aaggcagctt tttatgaggt acaaagataa agaaatcatt gttctgacgg
    16201 atctttttgg cggaagtgtc aacaacgaat tcttacaata tatcaatcgg gatcatgtct
    16261 atctcgttgc cggattgaat ttgccactat tgattgagct agtatctcgg ttggacgctg
    16321 aagtagagac aactgttttg atccagcagg tgctaactga ttcaaaaaaa atgatccagt
    16381 tttgtaacga gcggctcaat cagaaaatag aagaagacga attttaggag gaagaaaaat
    16441 gattgtattg acaagagtcg accatcggtt attacacgga caagtagctt tttcatggac
    16501 acaaacaata ggcgcagact gtattttgat tgctaatgat gatgtaccta cgaatgagat
    16561 acgaaagaca acgatcaaat tagccaagcc acaaggagtc aaactagtga tcaaatcaat
    16621 tgatgactcg attgccgcgt tgaaaagcgg ggtgacagat aaatataagt tattcatcgt
    16681 agtggaatcg atcgaggatg catataagct ggcagaagcg tatccagaaa tcaaaaccat
    16741 caacttagga gggatcaagg caaaacctgg tactcgcagc atcggaaaag cggtaaatat
    16801 tttaccagaa gaggaggaac gcttgaaacg attaattgac aaacaaatag aagtagaaat
    16861 ccgtcaagta ccagcagata aaaaagtgga tgtaagagaa gttttgtaag aaaggaagaa
    16921 cctcaatgat tgtacaagca gtcttattag gaattgttgc atttgtcgca caatcagaat
    16981 atgcactagg gacatcgttg ctttctcgtc cgattgtgac ggggctgttt acaggaatcg
    17041 tgttaggtga tgtaaaaact gggatcatta tgggcgcaac tttagagtta gcatttatcg
    17101 gatcgttctc agtaggtgct tcgattccac ctgatgttgt gaccggtggg attttaggga
    17161 cggcttttgc tattacagct ggagccggta cagaaaccgc gcttttacta ggtttgccga
    17221 ttgctacgtt gacattgata ttgaaaaata tttacttagg gcttttgatt ccaattatga
    17281 accataaagc agacaattat gcagaagaag gaaattataa aggagtcgag catatgcact
    17341 tgcttgccgg tatcggtctt tccttgatgc ttggtgttgt ggtaatggtt tcttatatgg
    17401 tgggaagcaa tacgatcgga aatctattaa atatgattcc tgacttcgtt caaaaggggt
    17461 tgtcagtagc tacagggttg attccagctc ttggttttgc gatgcttgct cgtttgctga
    17521 tcaacaaaca agttgcacca tattttttct taggttttgc tatagcttct tacttagaga
    17581 ttcctgtgac cggagtagct atttttggtg cgattttagc agttgtggta gtcaatatta
    17641 tgaatgtccg tcaactacca cttcaaacaa ctgcagggga ggtagaagaa gatgaagaat
    17701 tctgagatca aaaacagcaa aataacgaaa aaagagctaa atcacgtatt ttggcgttct
    17761 tttcaaatgg agttttcttg gaattatgaa cgtcagatga atatggctta tgtttatgca
    17821 atgattccga ttttgaaaaa gttatatcag tcgaaagaag aaatggcagc agctttgaaa
    17881 cggcatctag agttttttaa cacgacccca catatcgtta cactgatttt aggaatcacg
    17941 gctgcaatgg aggaagaaaa taaggatgat cctgaatttg atgtgactgc gatcgatagt
    18001 atcaaaacat ctttgatggg acctcttgca gggttaggag attctttctt ttggggaact
    18061 ttgcgattga ttgccacagg tgtgggtacc tctttagcca tgcaaggaaa tattttaggc
    18121 cctatcttgt tcattttgat tttcaatatt ccgcacattc tgtttcgcta tctagcaaca
    18181 ggttggggat atcgcttagg aacgggtttt ttgaaaaaga tccaggcaaa cggtatgatg
    18241 gaaagtttga cattaggcgc gtctattatt ggactgatgg tcgttggagc aatgactgcc
    18301 acgatgatca ccatcaatat tcctttaaaa atcggttcag gagaaaatgc tgtgactgtt
    18361 caaagtattt ttgatgatat cgttccaaat atcttatgtc ttggagcatt tggcatagtg
    18421 ttttatctat tgaagaaaga agtgaaacca ttaacgatct tgatcggact agctttcttt
    18481 ggaatttttg gttcgtggat agggattttt taaaaaagga gcagagaaaa tgtttacgat
    18541 gcaagattat atctatgaag aaaaggaagt actaagtacg atcttgaaaa agaatgattt
    18601 ttcgacaaga gaacatatga aaaaaacagt gaatttactg atcttagcaa cgggttcatc
    18661 ttataacgct tgtttagcag cgaagccagc attggaatct tatggtgatc ttactgtcga
    18721 tattcaagaa cctttttatt ttttcaatta tggaaaactt tctccttcaa tcgatactgt
    18781 gattgctgtg tcacaaagcg ggaaaagtgc ttctactgtt gaagcagtaa aaatgataca
    18841 aaaacaaggg ttacctgttg ttgctatcac aaatgacgtg caaagtccac ttgctttaga
    18901 agctgcacaa attattgatt taggtgccgg ggtagaatca gtaggcttcg ttacaaaagg
    18961 ctattctgcg actgttttac agttactact cttagggata ggaattggaa taagtaaaaa
    19021 taaaatcagc aaaaaaatag aacaagacta catgcaacag ttgcgcaaaa taatcaacca
    19081 tttaccagcg attattcaaa aaacggaaga attttttgat ggatatcaaa gtttatttcg
    19141 tttagcacag cgtttcgtca ccattggata tggtccaaac tggggaacag ctaaagaagc
    19201 agagacgaaa ttgactgaaa caatccgcgt tccgtcacaa ggatttgagt tagaagctta
    19261 tatgcacggc ccatacctgg aggcagatgc ttcgcatttg ctattcttta ttgaagggga
    19321 aagtgtaaat aaggaacgtt cgcaaaagtt gcaaagatat atgagtcggt acgttggaga
    19381 gactctcacc attaccacaa aaaaagcaag aaacgagaaa acacttggat tagcaataga
    19441 atgtgatgaa tatctgtctg tattagcgct tgtcgttccg tttcaactat tcgcttataa
    19501 aaccgcagta gctaaaggta ttgacttgaa caaaaagatt tttgaagact ttgatacagt
    19561 cttgaaaagt aaattataag aggtggaaat catgctgaaa ttcaatgaag aagaacaaat
    19621 caaagcaata aaaggcgcac tagctttacg tccgcaagta gaagaaatca tcgataaact
    19681 ttatgcggaa aaatttgacg ctgtctatta tctaggaatc ggtggaacgt atgcttcctc
    19741 gatgcaagct gtcacttata tgaatgggaa aagcaacttg cctgtctttg tacagcatgc
    19801 tgcagaatat tacactactg gaaacaaaag attaacaaaa gattctatcg ttattttgtc
    19861 ctctgttacc ggtacaactc aagaagtagt gaaagcagta gaacaaatca agaaagtagg
    19921 agcgacactg attggattta tcgataaagc gaacagtaag ttgtctcagc tatgcgattt
    19981 tgtggtcact tatccagctc ctggaacaga acaaatcaaa ttttttatgg cagcagatcg
    20041 attgatgtat ttgaatggag aattcgaagc ttattcagaa tactatgaac agctcgaacg
    20101 ttatttacca acagggttag tggaagcaga gaaaaaagca gatgcatttg gcttatcatt
    20161 tgcagaaaaa catcgacgtg atagtatgca ttattttatt ggagcaggga atcaatgggg
    20221 agctgtttat tcatatgcca tgtgttactg ggaagaacaa agctggcttc catcgaaatc
    20281 tatccatgct gccgagtttc ttcacgggac gttagagatc gtagaagaaa caaccccagt
    20341 cacattattt ttaggcgaag atgaacagag agtgttatct gaacgtgtgg ctaaactttt
    20401 gccaaaaatt tgcagcaatt atacattgat agacacaaaa gactatccag tagaaggtat
    20461 cagtgaaaaa tatcgcggac gtgttttgtc tttcttattg atgcatgtag tgacacaacg
    20521 catcgatgct catgtggagc agttgaactg ccatccatta gagatacgcc gctactatcg
    20581 tcagtttgat tattaggata gactggataa aaagaatata aaaaataaag agagcagcga
    20641 taactgctcc ctttattttt ttaaaatatt ggcatcaatt acgttataaa acacattttt
    20701 tgcattatct acagttcaac ttataatctg tttaatggga catgtgggtg ccaaccattt
    20761 tttgttatgc aatagtacta tttagttgtt gcatggatcc gtagtaaaag tccaagtaca
    20821 acgagttgcg ccagtttaca tagcgttttg tatccagaat gttgactaaa acaaaagttc
    20881 ctatgtcaac cgtcaacaga gacaatctgt ccgttgcagg acctgacaga tttagaaatg
    20941 ttcaatctgt ctcttatgta ttttaaatca atactcacta ggagaagttt cctaacctcc
    21001 taatagtaat gctggtagta acattgtcaa aactgcttcc tcttaggaac aagcaaagca
    21061 taacgagtgt aaaaataaaa tgtagactat tgtaacatat cattgattat ttcaagtaaa
    21121 aacgataaaa gaagtaaaca aacacttaaa aacaatggaa attagtccca actttaagaa
    21181 atgtatacta taatggaaat tatcaattat ttatttggag gaatatccat gaaacaaaaa
    21241 atcgcagttc ttggtcctgg atcttggggc actgctttag cacaaacatt ggcagaaaac
    21301 gggcatgatg tccgtatttg gggaaatgta cctgaacaga ttgatgaaat caataagtat
    21361 catacaaatc agcatttttt acctgatttg acgatcccag aatcgattat cggatataaa
    21421 gaattggcag aagcagtgga taacgcagat gctattttat ttgttgttcc aacaaaagcg
    21481 attcgttcag tcgcacagga acttattggt aaaatgaata ctaagccaat cattatacat
    21541 gcaagtaaag gactagaaca agatacgcat aaacgaatct cagaagtttt ggctgaagaa
    21601 attccagaag aaaaaagaca ggcaattgtt gtattatctg gtcctagcca tgctgaggaa
    21661 gtggcagtac atgatatcac aacaattaca gctgcaagta ttgatcaaaa agcagcagcc
    21721 tatgtacaaa aactgttcat gaatgattat ttcaggattt atacaaataa tgatgtgatt
    21781 ggcgtagaaa ctggtgcagc actgaaaaat atcattgcaa ttggtgccgg cgctatccat
    21841 ggattaggat tcggagatga tgcaaaagca gcaatcatga ctcgaggatt ggcagaaatc
    21901 agccgtcttg gtgtcgcgat gggtgccaat cctctgacat ttatcggttt gagtggtgtg
    21961 ggggacttga tcgttacttg tacaagtgtt cattctcgca attggcgtgc cggtaacttg
    22021 ttaggacagg gacataagct ggaagaagtt ctagaaaaca tgggaatggt cgttgagggt
    22081 gtggcaacga caaaagcagc agtagagcta gctcaacagc taggtgttga gatgccaatt
    22141 actcaaacaa tctataatgt tttgtataat ggagaagata tcaaaaaagc agcaaaagaa
    22201 atcatgttac gtgacggaaa aatggagaat gaattttctc ttcgttaaat agatgatcaa
    22261 tagttaatcg tcgtttagga ggaacagcaa gtgaaggtca aaaaagcagt cattcctgcc
    22321 gctgggctag gaacacgttt tttgccggca acaaaagcca tggcaaaaga aatgcttcca
    22381 atcgtggata aaccaacgat ccagtttatt gtggaagaag cattggattc gggaatcgaa
    22441 gacatcttga tcgtaacggg aaaagaaaaa agacccattg aagatcactt tgatgcaaat
    22501 atcgaattgg aaaacaattt gcgggaaaag gaaaaaacag aattactcgc tttagttgaa
    22561 gaaacgactc aagtcaatct tcacttcatc cgtcaatctc atccaagagg actgggggat
    22621 gccgtgcttc aagcaaaagc atttatagga aatgaaccgt ttgtcgtcat gctgggggat
    22681 gatttgatga aagatgatat cccattgact caacaattga tgatggatta tgaagaaaca
    22741 caagcttccg ctgttgccgt catgcgtgta ccagaaaatg aaacatctaa atacggaatt
    22801 attgatccta aaacagatag cgaaaagggt cgttgcagag taaaagggtt tgtagaaaaa
    22861 cctgaaatgg gaaaagcacc aagtaattta gccattatcg gacgttatct actgactcct
    22921 aaaatatttg agatattaga aactcaagaa ccaggagcag gaaacgagat acagctaacg
    22981 gatgcacttc agactcttaa ccagacagag gcggtctatg cgagagagtt taaaggaaaa
    23041 agatacgacg taggcgataa gttaggatat atgaagacaa atattgaata tggacttcaa
    23101 cattcggaaa ttgggacttc attaagtgca tatattatcc agttgagtaa aaagctaaga
    23161 acagaaagtt aactggaaat aagtggcctg ctgagaatga aaggctattt gttattaaag
    23221 cggcagcttg tgcgtcattt gcgcaactgt cgttttagtt ttttgactct agatcagttg
    23281 agtcgtggaa aacgaaagat agaaaaagac tttcgtgaat agattcagaa aatattatgc
    23341 taatggttta attttttttt aagttgtata aaagaaaatg attacataag agaaatgaaa
    23401 gccaagccag aagattcata gtagaataaa aaggcgaatt ttttaattat aaaaaaaggt
    23461 gtgtgttcac tatgggaaaa ttagtcgctg ttgcacagtc gctgcttgat atactcaagt
    23521 ggctgatgga aggaagcaat tggatatttg tgttgccact attgcttatt agtctattta
    23581 tcatgtttcg catggtcaga aaaaaagaaa cgtttctgat ttttatctgg tatgcagcat
    23641 ccgttggggt tgctaccttg atggttgaat tggcgttagg tagttgggaa gcttgcgtat
    23701 ttgctttatt ttttgctgta ctgtatctaa tcatttatat cccatatatg aaaagaaagt
    23761 ctcggatagg cagaaaaggt agacatacaa ctactttttc tagtacagat tctctcgaac
    23821 aaagtagaag aacgagaaga agacggtaat atttagtagg tatacgcaaa aaaagataaa
    23881 ataatggata aactattgag atgagagatt tatttaattg gtggaaaaaa gcttttttca
    23941 cttatataaa agaatctttt ttaaaggaat gcttgttgta tcgggataag ccattccttt
    24001 ttttatgtta tttatatata tataaatcat accaatgt
//
LOCUS       DACEPD010000040        23656 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.041, whole genome shotgun sequence.
ACCESSION   DACEPD010000040 DACEPD010000000
VERSION     DACEPD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.041"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            255..530
                     /locus_tag="IW797_001482"
     CDS             255..530
                     /locus_tag="IW797_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224265.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            543..2621
                     /locus_tag="IW797_001483"
     CDS             543..2621
                     /locus_tag="IW797_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1224266.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2781..4667
                     /locus_tag="IW797_001484"
     CDS             2781..4667
                     /locus_tag="IW797_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1224267.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="IW797_001485"
     CDS             4679..5626
                     /locus_tag="IW797_001485"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1224268.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="IW797_001486"
     CDS             5646..6173
                     /locus_tag="IW797_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1224269.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="IW797_001487"
     CDS             complement(6236..6889)
                     /locus_tag="IW797_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1224270.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="IW797_001488"
     CDS             7021..7863
                     /locus_tag="IW797_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1224271.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="IW797_001489"
     CDS             8021..8917
                     /locus_tag="IW797_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1224272.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="IW797_001490"
     CDS             8920..9633
                     /locus_tag="IW797_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1224273.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="IW797_001491"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="IW797_001491"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1224274.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="IW797_001492"
     CDS             10168..10395
                     /locus_tag="IW797_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1224275.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="IW797_001493"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="IW797_001493"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1224276.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="IW797_001494"
     CDS             complement(11181..11723)
                     /locus_tag="IW797_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1224277.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="IW797_001495"
     CDS             complement(11960..12358)
                     /locus_tag="IW797_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1224278.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="IW797_001496"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="IW797_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1224279.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="IW797_001497"
     CDS             13471..14238
                     /locus_tag="IW797_001497"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1224280.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="IW797_001498"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="IW797_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1224281.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="IW797_001499"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="IW797_001499"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1224282.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="IW797_001500"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="IW797_001500"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1224283.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18894..19799
                     /gene="xerC"
                     /locus_tag="IW797_001501"
     CDS             18894..19799
                     /gene="xerC"
                     /locus_tag="IW797_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1224284.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19836..20384
                     /gene="hslV"
                     /locus_tag="IW797_001502"
     CDS             19836..20384
                     /gene="hslV"
                     /locus_tag="IW797_001502"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1224285.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20398..21798
                     /gene="hslU"
                     /locus_tag="IW797_001503"
     CDS             20398..21798
                     /gene="hslU"
                     /locus_tag="IW797_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1224286.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21816..22607
                     /gene="codY"
                     /locus_tag="IW797_001504"
     CDS             21816..22607
                     /gene="codY"
                     /locus_tag="IW797_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1224287.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22689..23564
                     /locus_tag="IW797_001505"
     CDS             22689..23564
                     /locus_tag="IW797_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1224288.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8192 a         4079 c         4983 g         6402 t
ORIGIN      
        1 tttgtcgcag ccccattttt ttgttgtgat aaaagaacaa gaaaggcagg aatgggaaag
       61 taaacatcct tttgtcagaa acttttatct ttcttaagtt ttacttgata tctgatacta
      121 tttggaatag aatgaggcgt tgaaaaattt atggaaattt ctaactagca ataaacgatg
      181 aaacaatgaa atatttttca tccaaaaaga actttgctat aatgatggaa gtttgtgtat
      241 aggagatctg gaaaatgaga gagagttttg aacaacaaaa aaaactgttg catgatcgct
      301 atggcgccct ttcgatggat gaccgccgtc aaatactttg taagttgcgt aaaagaaata
      361 tattgatgta tcgtcagttg gaacgtctga aacatgatct gcttcgtttg gagtcaaaac
      421 gtgtacagtg tgaattagag gggaaccaaa cacaggttga agtagtcgaa accaaaattt
      481 taaagaaaaa agaacaattt ttaaaaatgc ttacacaaaa taaaaaataa aggggatgga
      541 cgatggaatt tgctgagtta gttattgtat ttgcagtaac gatcacctta tcgaacatag
      601 ctagccgcat tttgccaatg attccagctc cactgatcca aattttcctt ggagtggtat
      661 taggtctgac agagtgggga caatcaatcg attttgaacc tgaattgttc ttagttatga
      721 ttattgcacc attgttattt agagaaggag aaaaagcaga tatttcttcg attctcaaaa
      781 atttcgggac aattttgttc ttggcatttg gaggagtcat tttgaccctt gtcggggtag
      841 gtgcgacgtt atcattcctg ttgccaagtg tcccacttgc tgcctgcttt gcttttggag
      901 cagcgttagg tccgacagat gcggtagcag tcagttctct ttctggacga gtgaatatcc
      961 ctaaaaaggc gatgcatatt ttggaaggag aaggtctttt gaatgatgca tctggtgtta
     1021 ctgccttcca gtttgctctt ggtgcgctga tcaccggatc tttttcagca gtaaacgcgg
     1081 ggatgagtct agtagtttct agtattgggg gagctttgat cggctttttg cttgtctggt
     1141 tcaaacaaaa aataattcat ttgatcgaaa aagcatcagc acaagatgtc acagggtatc
     1201 tgctgatcga acttttgctt ccttttctag cctatgtatt ggcagaggca tttgatgtat
     1261 ctggaatcat tgctgcagtg gcagcaggta ttctccaagc ttctggtttt aggaaaatct
     1321 ctgtgtttga tgcagaattg tcgagcttgt cccacagtac atggacaact attgctttta
     1381 cactgaatgc attagtattt atatttttag gaatcgaatt gacgcaagtg ttctcgccag
     1441 tatggggaga cggtttatat ccaaacggtc ttttattagc gattatcgta ttaatctctg
     1501 tcatgctttt tgtgatcaga ttcatttcta tcagtctttt ttatgtattc aaagatggca
     1561 gtaaaaagtt caaaaaacag ttgaacgaga tcttgatctt aacatttggc ggtgtaaagg
     1621 gaacagtcag tttggctacg atttttattt tgccattttc aatcaataat atgatgtttt
     1681 accagcggtc tttgcttttg tttctgacgg cgggtgtcat cttagtgact ttggtcatag
     1741 ggatcattgt cttgcctatg ctgacggaaa cagaggaagc gaaaagcacg gatttgaatg
     1801 ctttgatgat tttagaagag gtcgttgaag ttttgcgaaa agaaataaag gaaattgatc
     1861 gtaatacaaa agaattttta gccactgaag cagtgataga aaattatcaa gaacggatac
     1921 gtgatctgta cttggaagat ttaaccgatg atgaaaagca agaagtacaa gaaatacaag
     1981 cactgatttt gtcgatcgaa cgggatggat tggatgaaag ttatcgttcg ggcaagcttt
     2041 cttctaatgg ttatcgtttt tactcccgtt ttctttctcg attcgaacat tctatcacaa
     2101 gccagatttt gtcttttatc ggcttttggt tcattgttgt ccgtaggtta atgcgaatcg
     2161 ttctccatcc gaaaatgttt tggcaaagaa gatatgctga tcgtcagaca tttattagtg
     2221 atgaagatat ccaagaaatc agagagacat atcaaaaaaa tactcagttg attattgaaa
     2281 gtttagataa cttgactgat atttatgatg aaacgatggt tcaatttttt atccagcaac
     2341 gcaaacttga aggaataaaa atgaactcgg ggaatttgat ttcttcgtgg atgatccagc
     2401 aagatagtct gtttacgaaa aaaatgctaa gaggttatta tcttgaaaga aaaatcattg
     2461 atgaatacga agttgctgaa aaaatcacga cgttttctgc gaacagctat cgcaggaata
     2521 tcaatttgtt agaatcttat acgatgaaca aaccatctga taacttttct ttccgatttg
     2581 cgtttcgagc aaagtcaaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcattttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacaccggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt cttgcgtttt tcttaagaat ttctcaaaca aaatgtgagg
    18781 aattgcgcca attgtgaaca aacttagaaa tattggttaa attctgacaa tttatttgtc
    18841 tttttataaa cgctgtgcta aattattctc tgggacaaaa ggaaggtgag aaaatgacag
    18901 aagccacgtg ggaacaaaaa tttttgcgtt atttgattgt cgaaagagga tattcagaaa
    18961 aaacgaaaga agcatatgaa gaagacattc tccatttcaa acattttttg gaggaatctg
    19021 gagacgctga tctgctgaag gcggaatatt ttacggttcg tgtctatttg agtgcattag
    19081 ctgatcatcg tcctagctat agcagaaatt ctatcagtcg caagatagcc agcctacgtt
    19141 cgttttatca gtatcttctc aaagaagaag tgataaaaga aaatccattt tcttacgttc
    19201 atctcaaaaa gaaaaatttg agactgccac gtttttttta tgaaaatgaa atgcaagcac
    19261 tttttgacag cgtagcagga gatagtcctt tggatttgag gaaccgcgct ttactggaag
    19321 tcctttacgg gtcaggcatc cgtttaagtg agtgtagcag tttgtcggta tcagacattg
    19381 attttgattc agaggtcatg ttgatccatg gtaaagggaa caaagagaga tatgctcctc
    19441 taggttcttt tgctcaagat gctctgcaag aatatttcag ggaaggccgc caggtattga
    19501 tgacaaaata tcacgaagag catgatatcg tttttgtgaa tcatcatgga aaaccaatca
    19561 cgcctactgg aattgaatat gtcttgaatc aaatgatcaa aaaaagcagt ttggatagtg
    19621 atatccatcc tcacatgctg cgccatacat ttgctactca tttattaaat aatggggcag
    19681 atttgcggac agtccaagaa ttgctaggac atgccaattt atcgactacc cagatctatg
    19741 cccatgtaac aaaagaaagt ttacaaaaaa actatcgttc tttccatcca agagcgtaaa
    19801 aataaggaaa agaacaaatt taggaggaaa caaacatggt cgaatcacca tttcattcaa
    19861 ccactatctg tgccgtcgaa aaagacggga aatttgctat ggctggagat ggccaagtaa
    19921 caatgggtga atctgttgtc atgaaaggca ctgcaaagaa agtacgacgt atctacaatg
    19981 gagaagttgt tgtaggtttc gccggaagtg ttgcagatgc gtttacttta gaagaaaaat
    20041 ttgaaggcaa actaaacgaa tataacggga atctacaacg cgcagctgtc gaattagctc
    20101 aagaatggcg gacacagcaa tctatgcaaa aacttgaagc gatgctgatc gtaatgaatg
    20161 ataaagaaat gcttcttgtt tcaggtacag gagaagttat cacaccagat gatggtatct
    20221 tagctatcgg atcaggaggg aattttgcgt tggctgcagc ccgagcaatg aaaaactatg
    20281 ctcaaaacga catgagtgcg aaagaaattg cagaaaatgc gttaaatatt gccgcagata
    20341 tctgtatctt tactaatcat aatattatcg tagaagaact gtaagaggtg agtagatatg
    20401 tatgaattaa aacaaacacc aagacaaatc gtaagtgaat tagatgaata tatcgtagga
    20461 cagcacgcag caaaaaaatc tgttgccgtt gctttgcgta accgctatcg ccgtttgcag
    20521 ttagatgaaa aaatgcaaca agatattaca ccaaaaaata tgctgatgat cggacctaca
    20581 ggggtcggta aaacagaaat cgcacgacgt ttggcaaaaa tcgtcaatgc accatttgtc
    20641 aaagtcgaag caacaaaatt tactgaagtc ggttatgtcg gaagagatgt agaatcaatg
    20701 gttcgggatt tagtagaaaa tgcgatccaa atcgtagaaa aagagcagta cagccgtgta
    20761 tacacaagtg ctaaaaaaaa tgcagaaaaa cgtttggtaa aaatcttggt acctggtatc
    20821 aaaaaagaac agaaacaatc cactaatcaa tttgaacaaa tgatgaatct ttttaatgct
    20881 agtcagcaaa ataccgctga accgcaagaa gaagtgacag aagatattcg aatcaatcgc
    20941 cgtacagtct tggaacaact acaaaagggt ttactggata gtcgtgaagt aaccattgaa
    21001 gttgaagacc aaaagaaacc tgcaccaatg atgaacaatg gattggagca aatggggatc
    21061 gacctaaatg aaactctagg tgctttgaca ccgaagaaaa aagtggaacg gacggtaaca
    21121 gtcaaagaag ctttagagtt attgatcaag gaagaatcag caaaattagt caaggatgct
    21181 gatatccata gtgaggcaat caaattagcg gaagaaagtg gaattatttt tatcgatgaa
    21241 atcgataaga tcacttctaa atcacagcaa tctggagaag tatccagaga aggcgtccaa
    21301 agagacatat tgccaattgt cgaaggttca caagtaaaca caaaatacgg gatcatccaa
    21361 acagatcacg tcctattcat tgcttcagga gctttccatt tgtctaaacc aagtgatcta
    21421 attccagaac ttcaaggacg cttcccgata cgggtagaat tagatgattt gacagctgaa
    21481 gattttgtga agattctgac cgaaccaaat aatgctttag taaaacaata cgtcgcgttg
    21541 atcggtacag aaaatgtgtc agttacgttt acaaaagaag caattgaacg gatcgctcaa
    21601 atcgctttcg atgtcaaccg tgacacagat aatatcggcg cacgcagact gcataccatt
    21661 ttggaaaaac tactagaaga tctattattt gaagcaccag atatgcaaat gggagaaata
    21721 acgatcacag aagcatatgt ggatgacaaa cttggctcaa ttgcggcaaa tgaagattta
    21781 agtcgctata ttttataaaa aatcggagga cgacaatgac agacttgcta acgaaaaccc
    21841 gcaagataaa cacacttttg cagcaaaaga atacttttga catgcaagca gatttgcctt
    21901 acgataaaat ggctgtcaca ctaggtggta ttcttgacag caacacctat atcataggaa
    21961 gtgacggagt attactaggt tataatgaac gtcacgatgt gaacaatgaa cgaatcaaaa
    22021 caatgtttgt tgagaaacaa tttcctgaat cttatactga tacagtggca cagttgatga
    22081 aaactgaagc taatatccca attactagtg atattactgc atttccggta gagtcaagag
    22141 agaaatatcc atttggtctg acgactgtcg ttccgatctt tggagctggc gaacgtttgg
    22201 gtacgattat cttatctcgt atggaacaat cgtttgcaga tgacgatcta gtcttagcag
    22261 aatatggagc tacggttgtc ggtatgcaga tcctttatca gaagtctcga agcatcgaag
    22321 cagatgtacg gagtgcaacg gctgtccaaa tggcaatcaa cacattatct tatagcgaat
    22381 tgaaggctgt acaagctatc tttgaagcat tggatggaga tgaaggtcgt ctgactgctt
    22441 ctaatattgc cgatagtatc ggcatcactc gttctgtgat cgtcaatgct ttacgaaaat
    22501 tggaatcagc tggaatcatt gaatctcgtt cgttaggcat gaagggaact tatttaaaag
    22561 tattgaattc ccgttttaaa gacgagttag caaaacacag ttattagaag taaggggact
    22621 ggggcagata gaaatgtttg ttccagcctt cccattttaa aaagtaagtc gaagttggag
    22681 gaatggcaat gactgttatt attcaaaatg accagctgat cgccgaaatc gctgaacacg
    22741 gtgcggaatt gatcagtctg aaatcaaaag aacatggttt agagtatatc tggcaaggtg
    22801 atccagcgta ttggggcaga catgcaccgg ttttattccc ttttgttgga cgtttaaaag
    22861 atgatcagta tgtttatcaa gggaaaacat atcaaatggg gcaacacgga tttgctcggg
    22921 atatggattt tgaagtcatt gaacatggac aagaaatggc tagtttcatc ttaaggagta
    22981 cgccggagac attagaaaag tatccatttg attttgaatt agtgatcagc tatgaattag
    23041 gaggagacgg catcacggtt cattatcaag tggcaaatac cggtgaaaaa gaaatgttct
    23101 tttctatcgg tggacatcct gctttcaatg tacctcttga agaaggattg tcatttgaag
    23161 atttctatct ggcattttct ccattgaaat caagattacg cctaccattg aaaggcccat
    23221 tcatcgatat ggatcaaaag acgttaggtc agacaaatac gaatctggct ttgaatcgtg
    23281 aactttttga gcaagatgcc ttggtttttg aaacaagggg tctaaatgcg tacgccattc
    23341 gaagtgaaaa atcttcccat agcgtaactt taagctataa agatatgcct tatgtaggga
    23401 tctggtctcc ttatccaaaa gaagcgcctt tcgtttgtat cgaaccgtgg tgcggaatcg
    23461 ctgacacggt gaacagtagt ggaaacttga tggaaaaagt agggatcaat caactaggtg
    23521 cccatgaaat cttcaagaca aaatattcca tcacagtgaa gtaagaggtt gtgaccgaga
    23581 agttagcagg gaacaccgtt taccaaggtg tgtaaaaaag ctatcctgta agtaataagg
    23641 gagtgtgaca tcgatt
//
LOCUS       DACEPD010000041        23538 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.042, whole genome shotgun sequence.
ACCESSION   DACEPD010000041 DACEPD010000000
VERSION     DACEPD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.042"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1751
                     /locus_tag="IW797_001506"
     CDS             <1..1751
                     /locus_tag="IW797_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1224289.1"
                     /translation="GIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALIGMIPEGLYL
                     LTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMSVQKVIVSKK
                     QEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPFSSVQKFSSV
                     ALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEEPVLTEAVTP
                     LGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGISHADQYVDAS
                     KLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDILAMKEADCSI
                     AMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVKNIFSFLLSL
                     FSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVMEKAVPGGLT
                     DMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFRSQIILFCAS
                     GNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFERKNEKEQKP
                     FSRVAKKFLSIFKREN"
     gene            complement(1854..3590)
                     /locus_tag="IW797_001507"
     CDS             complement(1854..3590)
                     /locus_tag="IW797_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ1224290.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(3587..4291)
                     /locus_tag="IW797_001508"
     CDS             complement(3587..4291)
                     /locus_tag="IW797_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1224291.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            4422..4925
                     /locus_tag="IW797_001509"
     CDS             4422..4925
                     /locus_tag="IW797_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ1224292.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            4885..5223
                     /locus_tag="IW797_001510"
     CDS             4885..5223
                     /locus_tag="IW797_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ1224293.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            5244..6662
                     /gene="ffh"
                     /locus_tag="IW797_001511"
     CDS             5244..6662
                     /gene="ffh"
                     /locus_tag="IW797_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ1224294.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            6774..7352
                     /locus_tag="IW797_001512"
     CDS             6774..7352
                     /locus_tag="IW797_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ1224295.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            7356..7877
                     /locus_tag="IW797_001513"
     CDS             7356..7877
                     /locus_tag="IW797_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1224296.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(7956..8510)
                     /locus_tag="IW797_001514"
     CDS             complement(7956..8510)
                     /locus_tag="IW797_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1224297.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            8763..9038
                     /gene="rpsP"
                     /locus_tag="IW797_001515"
     CDS             8763..9038
                     /gene="rpsP"
                     /locus_tag="IW797_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ1224298.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9050..9301
                     /locus_tag="IW797_001516"
     CDS             9050..9301
                     /locus_tag="IW797_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ1224299.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            9426..10217
                     /locus_tag="IW797_001517"
     CDS             9426..10217
                     /locus_tag="IW797_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224300.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            10387..10908
                     /gene="rimM"
                     /locus_tag="IW797_001518"
     CDS             10387..10908
                     /gene="rimM"
                     /locus_tag="IW797_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ1224301.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            10898..11659
                     /gene="trmD"
                     /locus_tag="IW797_001519"
     CDS             10898..11659
                     /gene="trmD"
                     /locus_tag="IW797_001519"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ1224302.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            11795..12142
                     /gene="rplS"
                     /locus_tag="IW797_001520"
     CDS             11795..12142
                     /gene="rplS"
                     /locus_tag="IW797_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ1224303.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            12515..13177
                     /locus_tag="IW797_001521"
     CDS             12515..13177
                     /locus_tag="IW797_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224304.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            13256..16483
                     /gene="fss3"
                     /locus_tag="IW797_001522"
     CDS             13256..16483
                     /gene="fss3"
                     /locus_tag="IW797_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ1224305.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            16597..16911
                     /locus_tag="IW797_001523"
     CDS             16597..16911
                     /locus_tag="IW797_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1224306.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            16924..17298
                     /locus_tag="IW797_001524"
     CDS             16924..17298
                     /locus_tag="IW797_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1224307.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            17308..17652
                     /locus_tag="IW797_001525"
     CDS             17308..17652
                     /locus_tag="IW797_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ1224308.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            17723..19075
                     /locus_tag="IW797_001526"
     CDS             17723..19075
                     /locus_tag="IW797_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ1224309.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            19135..20277
                     /locus_tag="IW797_001527"
     CDS             19135..20277
                     /locus_tag="IW797_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAQ1224310.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            20302..20556
                     /locus_tag="IW797_001528"
     CDS             20302..20556
                     /locus_tag="IW797_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224311.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            20812..21996
                     /locus_tag="IW797_001529"
     CDS             20812..21996
                     /locus_tag="IW797_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1224312.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            22011..22130
                     /locus_tag="IW797_001530"
     CDS             22011..22130
                     /locus_tag="IW797_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ1224313.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            22135..22356
                     /locus_tag="IW797_001531"
     CDS             22135..22356
                     /locus_tag="IW797_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224314.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            22369..22869
                     /locus_tag="IW797_001532"
     CDS             22369..22869
                     /locus_tag="IW797_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1224315.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            22966..>23538
                     /locus_tag="IW797_001533"
     CDS             22966..>23538
                     /locus_tag="IW797_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAQ1224316.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYD"
BASE COUNT         7744 a         4144 c         5111 g         6539 t
ORIGIN      
        1 ttgggatcat cattattcct atcggtatcc tgcttttttc tcaaagttac ttttttaatg
       61 gaaacacgat caaagaaagt gtcgttgcaa tggaagcagc attgatcggt atgatacctg
      121 aaggactgta cttgctgacg acgattgcat tagcattaag tgcgactaaa ctagcaaagc
      181 aaagagttct tttacacaat atgaaaagca ttgagacatt ggcaagggta aatgtccttt
      241 gtgtagataa aacaggaacg atcacagaaa ataaaatgtc tgttcaaaaa gtgatcgtct
      301 ctaaaaaaca agaaataatc agtcagtcag atcaattgga agcatgcatt gctgactatg
      361 caaaagcgat ggcaggagac aatgcaacaa tggaagctgt taaagcatat tttacaaaaa
      421 ccactgatca atcctatcac catgttattc ctttttcttc tgtccaaaaa ttcagcagtg
      481 tagcgttggc agacaaggtg tatgttttag gagcgcccga aatggttttg cgcgatcaat
      541 tgccggaata tgcggaagag tttgtttctt tcgctgaaaa aggctatcgc gtgttggtct
      601 ttggtgtgta cgatgggatt cttgaagaac cagtattgac agaagcagtc acaccattag
      661 gctatattct gattgctaat cctatcagaa aagaagcaag agccacattt gattacttca
      721 aaaaacagca agtagccatc aaagtgattt ctggagataa tccgctcacc gtatccaatg
      781 tagctgtcca agcagggatt tcacatgctg atcagtacgt tgatgcatct aaattatcag
      841 aagaagaata tgaagcagct gtcgaaaaat atacagtttt tggacgcgtc aagccagaac
      901 aaaagaaaat cttcgttcaa ttgataaagc gtaacaatac agtagctatg acaggtgatg
      961 gcgtgaatga tattctagcc atgaaagaag cagactgcag tatagcgatg gcttccggaa
     1021 atgaagccgc catgcaggcc tcacaagtgg tcttacttga ttctgacttt tcacgcatgc
     1081 cagaagttgt tgcagaagga cggcgcgttg tgaacaatat tgagcgatca gccagcttgt
     1141 ttttagtgaa gaatattttc tcctttttgc tctcactgtt ttcagtgatc tttgctctga
     1201 cctatccttt agaaccatca caaatcacat tgattagttt gtttactatc ggacttccat
     1261 ctttcttgct tgctctagaa gaaaacaaaa agcgaatcag agggaaattc atcatgaatg
     1321 tgatggaaaa agctgtgcca ggtggactaa cagatatgat cgtagtcggt gcgttggtga
     1381 tctgcggggt gactttgaat ctaaataaaa cagatgtttc tacagcatct accatgcttt
     1441 tgattgcagt agggttcctc gttctctata aaatctgttc accgctgaat aaattcagaa
     1501 gtcagatcat tcttttctgt gctagcggta actttttttc ggtcatcttt ttgcataagc
     1561 tgttttcaat caccggtatc tcagctgtat cacttttgct gttgagcatt ctatttttct
     1621 cggcagattc gatttttagg catttgacca cattggtaga atatttgttt gaacgtaaaa
     1681 atgaaaaaga gcaaaagccg tttagtcgcg tagctaaaaa atttctatcg atatttaaac
     1741 gagaaaattg acgaaaagta tctcaaaaaa gagtaaaaaa gaacaaccac tcgtgtatct
     1801 ttcgaagcag gatgagtcat ccaactttaa aacgcacgag cagttgctct tttttacatt
     1861 ttcggaatgg taacggtgaa agtcgaacct gttccaagat gactgtctac agtaatcgta
     1921 ccattaagga gttgcacgta gtcttttaca atcgctaacc ctaatccggt cccgcctgaa
     1981 tgtcggcttc gcgccttgtc tacgcggtaa aaacgctcaa agatccgttc ctgatcctca
     2041 atgtcgatac ctataccaaa gtcttggaca gaaaacgata gatcatctgt tgcctgatag
     2101 cggataataa tctcgctgtc gctttttgaa tattggacag cattttcaat cagatttttg
     2161 ataattggat aaaggatatc tgttttagta gtaaatggtt cgttctttcc aatcaatcga
     2221 atcgtcaacc gtttcttttc gatgattggc tgataatttt gaacgatttg ctggaaataa
     2281 ggttctgtat aaattgtctg gatctcgtaa gaattctcgc catcttttga cagctggatg
     2341 atttcccgaa tcagtttatc taaccggatc gcatcttttt gcatgatctc aagaaaagaa
     2401 gtcagagttt gcgggtcatc ttttgcacca tccagtaaag tctcggtaaa accaatcagt
     2461 gaagtcacag gagtttttaa ttcgtgagag acgttgccaa cgaaatcctt ttgtagcttt
     2521 tctaaacgtc gcactttcgt taagtcatac gccaccccta gaatctgtcc ggtaccttct
     2581 gaattagtaa agtagcgcaa actcaagtct aatgtctgcc gaccttctgt aagggtgatc
     2641 tcctgatgga tcagcggatg ctcaggagtc acttgatgaa tcagctgaat gagttttggc
     2701 tcttgaatga cttctgtgta ccgctgttca ggttgatagc ttaccacgtt taaatgtagc
     2761 tgcatctttg gattaagcag tagtaaacga gaatcagcat catcaatcag gaatacgcca
     2821 atcatcagtt cgttcagtaa cgtatataat tgctcttctg tggtagtaca cgcccgatac
     2881 atcttactca tttgttcgct caacgcatta atcgtctgat acaattcttc ccattgatcg
     2941 ggtgtttgca tgattacttc cgcctgatta ggattttgca ccattttttt tagtacgggc
     3001 agcacggttt tcaatggctg gtttttttga tagatcagat aatagatcat tcctgcaaga
     3061 acaaggaaga aagacagaaa gaagaaaaag acccaacggc gaaaactctc agttctcggt
     3121 aaaaaaccgc ttgtcggttc tgcgatacgt aaaatggctt ctaactggcc actttttttg
     3181 actggtaatg cgacatataa aagatcctca ttcaaagtag tacttttgcg caaagcgcta
     3241 cctaggttcc caccattcaa tacggctttg atttcgggac gggagtttcg ctgttcattc
     3301 aatgtttcat tgtttgtgtc aaataagatc tttccagtag catccattaa agtaatccgt
     3361 tcgttcgact gatggacgaa ttcttccaaa gcagttttgt tttgcgctgc ttggaggttt
     3421 tcgacatcta attgccggat cagcagttgt gctttttttg tcaggtagct ttcttgctgt
     3481 tccacgatct gctgttgaaa ataatgggaa atcaattgcc agccaccaaa aaacaaacta
     3541 actaaaagaa taagaaagaa gagacgttca taaatttttt tccatttcat cgttttggtt
     3601 cctgaaactt atagccaaat cctctaatag tcaccaagta ctttggatgt tttggatctc
     3661 gctcgatttt ttctcgcaaa tgactaacgt gtacatctac gattcggctt tgtcctgcga
     3721 agtcaaaatt ccaaatgcgg tctagcaatg tgtctcggtc gatcacacgg tcttttcgtt
     3781 tcatgaaata aacaagtaat tcaaattctt tcggtgttag ttcaatcaga tggtcgtcta
     3841 cggtcacttg ataattggtc aaatcagcag tgatttgtcc aatcgataga tattcaggct
     3901 cagcttcatc ttttgattgc ttacgaggtt caatccgtct gaatattgct ttcatacggg
     3961 ctaatacttc gcgaggacta aatggtttgg tcaaatagtc gtcagcacct atttctaggc
     4021 caatgatccg atccacttgg tcatctttag ccgtcagcat caaaatcggt gtttcatttt
     4081 tttcttgacg cagccgctga gtgatctcca tgccatccat cgttggaagc atcacatcta
     4141 aaatgatgaa atcaaatgat tgttctaatg ctaattctaa tccttcgcct ccatctgtgg
     4201 cactggttac cttgtagcct tctttttcca aattgaacgt caagagcgtt acaatagaag
     4261 gttcatcatc aacaactagt atttttttca ttcaaatctc ctttaaatag gtttcattct
     4321 gtttatattt tagcatagaa tcagtacaaa gggctatttt acaaaagaaa taaatggtat
     4381 aataggaagg cactttgctg aatagaaagg aagagcagtc aatgattgga atcagtgcat
     4441 gtttaggcgg tctggcctgt cgctatgatg gacagaaaaa agaagtacca gaattgatgg
     4501 aactagttga aactggagaa gctgtcatgg tctgtccaga agtgatcggc ggattaccaa
     4561 ttcctagaga tcccgctgaa atcattggtg gaaatggatt cgatgtgtgg aaagatcaag
     4621 caaaagtatg gacgacaagc ggcgaagatg taacggaagc gtacaaaaat ggcgcgatca
     4681 aagcctatca aaaattgcag gaaaaacaca ttgatctagt catcatgaag gaaaaaagcc
     4741 cttcatgcgg aacccattcg atttatgacg gaagtttttc cggaataaaa aaaaatggtg
     4801 ccggagtggc tactgcttat tttattcagc aaaaaatgac tgtgctctct gatgaagaat
     4861 ggctgaaaac tagaggtgag cagaatggaa atcgaaaaga ccaatcgaat gaacgcgctc
     4921 tttgaatttt attcgacctt attgacagaa aaacaaatga actacatgga attatattat
     4981 gcagatgatt tttcattagg tgagatcgcg gaagaatacg aagtaagccg acaagcagta
     5041 tatgacaata tcaagcggac aagcaagatc ttggaggatt atgaaaaaaa actccatctt
     5101 ttttctaatt atattgttcg agaacaggta ttggaaaata tgactgcata tattacagat
     5161 aagtacccgg aagataaaaa actacttgaa tatgtacagc aaattcaagc aatcgaagaa
     5221 taaataaaag gaatgaagaa aatatggcat ttgaaagctt aactgaacgt ctccagcagg
     5281 cgatgggaaa aataagaaaa aaaggaaaag tatcagaagc tgacgtaaaa gaaatgatgc
     5341 gggaaatccg ccttgcctta ctagaagccg atgttaactt gcaagtagtc aaagatttta
     5401 ctaagcgcgt acgcgaacgt gcggtagggg cagaagtgct ggaaagttta tcacctgcac
     5461 aacaaatcgt aaaaatcgtt gatgaagaat tgacgatcac acttggttct gaaacagcag
     5521 agttaaataa atcaccaaaa ataccaactg ttatcatgat ggctggtctt caaggggcag
     5581 gtaaaacgac ctttactggt aaattagcca attatttaaa gaaaaatgaa aatgctcgtc
     5641 cgctcctaat cgcaggggac gtatatcgac cagctgcgat cgaccaattg aaagtattag
     5701 gtcaacagct agacgttcct gtatttgata tgggaactga agtcagcccg gtagagatcg
     5761 tccgtcaagg gatggagtta gcaaaagaaa agaaaaacga ctatgtattg atcgatacgg
     5821 caggacgtct gcatatcgat gaaacattga tggatgagtt gaaacaaatc aaagaattga
     5881 cgcaaccaaa tgagattttg cttgttgtcg atgccatgac tggtcaagat gccgtcaacg
     5941 tagctgatag cttcaaccag caattaggta ttacaggggt cgtcatcact aaacttgatg
     6001 gggatactcg aggcggggca gcactatcga tccgctcagt gacaggcgca ccaatcaaat
     6061 tcatcggttc tggtgaaaaa ttgactgact tagaagtatt ccacccagat cgtatggcta
     6121 gccgtatcct tggtatgggg gatatgctga cattgattga aaaagcgcag caagactacg
     6181 acgagaaaaa agcggaagaa cttgcacaga aaatgcggga aaattcattc gatttcaatg
     6241 actttattga acagcttgat caagtgatgg gtatgggccc actggaagat ctaatcaaaa
     6301 tgatcccagg aatgaatcaa gtccctggaa tcgaaaacgt caaggtagat ccaaaagatg
     6361 ttgaacgcaa aaaagcgatg gtttattcta tgacgccagc agaaagagca aatcctgatc
     6421 tgttgaaccc aagccgtcgt cgccgaattg ctgccggttc tggaaacagt gttgttgaag
     6481 tgaatcgaat gatcaaacaa ttcaaggaat caagaaaaat gatgcagcaa atgtccaaag
     6541 gggatatgaa catcccaggt atggaccaaa tgtttggttc aggcgtcaaa ggaaaactag
     6601 gcaaaatggc gatgaatcga atgatcaaga aaaataagaa gaaaaagaaa aaacggaaat
     6661 aagatgaaaa aaacagcttc ggtacagact gaaaatgtct actgccggag cttttctatc
     6721 agcaattttt attctttact aatacaatta ctctaaacga agaggtggca cagatgagat
     6781 gtccgtggag tgaacaaaca gaaagtatgc ggcaatatca tgatactgtc tggggtgttc
     6841 ctgaatatga tgaccaaaag ctgttccgaa aattgatgct tgatattaat caagccggat
     6901 tgagctggca gacgatttta aataaaagtg aagcattcga tgaagcatat gatcgattcg
     6961 atatcaaaaa agtcgcaaat tatggagaaa caaaaatcag tgaactaatg gaaaacaaag
     7021 gaattattcg taaccgaaga aaaatcgaag cagccgtcca aaacgccaaa gcagtcttga
     7081 aaattcaaga agaaatcggc agtttttcag agtatctttg gtcgttttct aatggaaaaa
     7141 tcgttgattc ttcttatcat tcacaagaag aagtcccaac aacaagtcct ctttcagatg
     7201 aattagcaaa agatctaaag aaaagaggat ttaaatttat cggaagcaca acaatctatg
     7261 cttttttaga agctgtcggg atcatcaatg atcaccttga cagttgtttc cgcaaagaag
     7321 aggtcgtacg tatcggcaga aagggcgaat aaagcatgcg aaaaatcata ttttatggtg
     7381 ctataagttt agatggctat ttggcaacga aaacggatga tcttcaatgg ctttttgaca
     7441 ctccaacagg taagcaaact acttatgaag ctttttatga aacaatcgat acgaccatca
     7501 tgggcagaaa aacttatcag gaagcaaaaa aatatttgga gaccgaagca atttatccag
     7561 agaaaaagaa tttcgttttt tccacccacg cacaattaca attgccagat gctgaggtcg
     7621 taaacgaaga tcctgttgct tttctaaaaa atctacaaat gacagaagga aaagatatct
     7681 gggttgtggg tggcggtcga ttgatcaaac cgctattgga acatcaaatg attgatgaat
     7741 ggtatatcca gattacccct gtattattag gggacgggat tcctttattc caaaaaggaa
     7801 catatgaagc gcgttttgaa atgctagaca cgacacgatt cggtgaattt atcgaattgc
     7861 attatgtacg aaagtagaag taaataaaat aaaaaaggca actatatgca gaaatgtcct
     7921 gtacatagtt gcctttttta ttaccgttat gctttttact ctgttaattt gttagaaaaa
     7981 tctagatacg catctaaaat cgtttggaag aaacttaatg ttccttcaac gatattacca
     8041 ttttcatcaa ttagattcgc aacgtttccg atatatgctt ccggttgctg caaggttggc
     8101 acattcaaaa agacaagtga ttggcgtaga tggtggtttg cgccaaagcc gccgatccca
     8161 cctggtgatg cagtaacgac taaacctggt ttatgatccc aaacactgtg tccataaggt
     8221 cttgagccta catctaaagc atttttcaat actgctggca cagaacggtt gtattcagga
     8281 gtaacgaaaa cgacaccgtc tagtcccctc acttcctcac gaaaacgagt ccattcagca
     8341 ggagcctgtt ctggagtttc gagatcctca ttgtaaaatg gcagatcatc gatttttaca
     8401 aatacacttt cgtacccttc aggaagcaat ttcgcaaaag cttctgctac ttttttgttg
     8461 taagaatctt ttcttaaact accaataaaa aatccaattt tttttgtcat acgatttttc
     8521 ctccttcaaa ctatctataa taatcttaca aaagataagt aactggagca aataaaaaca
     8581 cttactgaca aaagctacat agaagataga ttacttattt ttaacaaaaa agaagtctaa
     8641 aatcttctat tttctaaaaa aaatcgaatt taatcaagct gtaaagaaaa aaagctttac
     8701 agggtaaaaa aaaactgtta cactacatct tgtgaataaa acaatggagg tggaaataaa
     8761 taatggcagt taaaatccgt ttaaaacgta tgggttctaa aaagagtccg ttttaccgta
     8821 tcgtcgtagc tgattcacgt tctcctcgtg atggacgttt catcgaaact gtaggtactt
     8881 acaatccttt gaaagaccct gcagaagtag ttttaaaaga agacttagtt cttgactggt
     8941 tatcaaaagg tgcacaacct tctgacactg tacgtaacat cctttcaaaa gaaggcgtta
     9001 tgcaaaaaca ccacgaagct aaattctcaa agaaataagg tgaccggata tgaaagattt
     9061 aagcgactta gtcttaacaa tcgttcgtcc gctagtcact tatcctgatc aagtcaagtt
     9121 agacgtcgta gagtcgaaag acttttacga gtacaatcta acagtggctc ctgaagatat
     9181 tggccgtatc attggaaaac aagggcgtgt tgctaaagca atccgcacaa tcgtttacgg
     9241 tgttcggatc aatgcaccga aaaaagtgcg tttgaatatc atcgatgata aggaaaagta
     9301 aataaacgtg ctgtactcga attttcgagt gcagcttttt tgtttttttt attaattgaa
     9361 gaaatgatta tttggcaaaa caagcaagaa agcgtatcct tataggagga gggagtgaac
     9421 agccaatgag aaagaaaatg ttatttattc catttgctct attgttaagc gcttgtagtt
     9481 cggttactca agaatcagac aatacacatc tatcaagcag tatatctgaa actgttacca
     9541 gtccagaaaa aagtagtacc aaaacggctt ccaccactga acaaacaatg gctacctcaa
     9601 ataatgagaa aaagactgct ttagaccagt tgaaagaaca acagccaaat gttcccatgc
     9661 ctctagatgt accagtctct tcaggctatc tcaatatcgc agcgacacat acaaagcaag
     9721 gttattctat tttatattac agaacagatc gtcccctcgg gttgaatgct gacgaattga
     9781 atcaagaaac gcctattgca acctatctat atcaatatgg gtttgcttct agtcaagaaa
     9841 caatccaagt tttgcagcca tttgagatcg acacaaatgg tcagcaagtg gacttaggtt
     9901 ctcgaatcac agggtatcag caaggtgcag caggttcaag ttttcttgaa tggcaagaag
     9961 gaaactggcg tattcgaatc cgtgggaata atatagaagg ccaagatccc ttactattag
    10021 ctaaagaaat cgtagcttat ttggaagaaa acagtctacc agcacctgaa caatttggta
    10081 aaattactgt tgatatgggt gatacgacga atcgagccgt agaagtaagc tggcaagaac
    10141 cgaaaaatgc ctatacgatc actcatcaag atcctatgtc tgcgttgaag atggcagttt
    10201 ctatgaagag gttgtgaaat cagcgcgctg atctgagccc atcatttaac tccaagtcac
    10261 aggaagtaat caaaaatagc acgccgcatt tttgcataat tttggcttaa taccttaaaa
    10321 gaaaactaga attgcctctt cacacatgct aaaataaaac agacaaaaaa gaaatgggga
    10381 tataagttga ctgaatactt gaatgtagga aaaatcgtaa acacacaagg tatcaaaggc
    10441 gaagtccgtg ttatctcaac aacggatttc ccagaagaac gttacaaaaa aggaacggtg
    10501 ctaacactat tccaagacgg aaaagcacct gtggaattaa cagtcaaaag tcatcgcaaa
    10561 cataaaaatt ttgacttatt gagttttgaa ggacatcctt ctatcaatga tgtggaaaaa
    10621 tatcgggatg gcatattaaa agtgtcaaaa gacaactctg tagatctagc tgaaaatgaa
    10681 ttctattacc atgagatcat cggtctgcat gtattagaag atggaactga gttaggtaaa
    10741 gtaaaagaaa tcttgtcacc aggtgccaat gatgtttggg tcgttcaacg tccgaaaaaa
    10801 gctgatatat tgcttccata tatcgcttct gtcgtcaaag aagtcgattt agaagctgga
    10861 gtggtccgtg tagaaattcc agaaggactg attgacgatg aagattgatg tattgacttt
    10921 atttccaaga atgtttgaag gtcctatggg ggaatccatc atcggtaagg ctgtagataa
    10981 aggattatta gatatcaata tctctaactt ccgcgattat tcagataaca aacatcagac
    11041 agtagatgat tatccatacg gcggtggagc aggtatgctg ctcaaagtcc agcccatcta
    11101 tgataacatc caagccattg aaaaagctgc accggatgtc aaaaaaagag taattcttct
    11161 tgacccagct ggtaaacgat tcgatcagaa aatggcagaa gaattttcac aagaagaaca
    11221 cttagtattc atttgtggtc attatgaggg atatgatgaa cgtatccgca cattagtgac
    11281 agatgaagtt tccttaggag attatgtcct tactggggga gaattaggtg cgatggtcat
    11341 gatcgatgca acagttcggc ttttgccaga tgtgttaggg aatcagactt cagctcaaac
    11401 ggattcttat tcaaccggtt tattggaaca tccccaatat actcgcccgg cagaatataa
    11461 agggatgaaa gtaccagaag tattgacaaa tggaaatcat aaattgattg aagaatggca
    11521 attaaaagaa tcactaagaa ggacctatca acgtcggcct gatcttttgg aaacacttga
    11581 atggacacct caaatgacga aatttttaga agaaatcaag aaagaagaac aagaaaaaac
    11641 agcagatgaa gccaagtgac tttttctaaa atcttcttta cagtaggttg ataatatgtt
    11701 agtattttat ggtgagtgca aaagcactta actattacga tattccgctg tgagaaaagc
    11761 tcataagaat atttggaata aggagaagaa aaccatgaat ccattaatcg aagaattaac
    11821 aaaagaacaa ttacgttctg acatcccagc gttccgccct ggtgacactg tgcgtgttca
    11881 cgcaaaagtt gttgaaggta ctcgtgaacg tatccagtta tttgaaggtg ttgttatcaa
    11941 acgccgcggt gctggaatca gcgaaactta tacagtacgt aaagtttcta acggagtggg
    12001 cgttgaacgt acattcccat tgcatacacc acgtgttgca aaaatcgaag tagttcgcta
    12061 cggtaaagta cgtcgtgcaa aattgtacta cctacgtgca ttacacggaa aagctgctcg
    12121 tatcaaagaa atcagaagat aaacacggaa aacttccttg gaaatctgaa atttgtgaaa
    12181 agtccttgta tatcaagggc tttttctttt ttctctatca cagttttaag ccatttttgg
    12241 ctgttttgga ccatttttca atcatattcc cttaaaaatt gtcctagatt gtccgacatt
    12301 gactatcagt gtcgtcaaaa tagggcaaaa tttagtagtc agcttgacga caaatataat
    12361 acgaaaaata ccaaaacatt taccgaattt agcaactttt gaacccaagt ttctcaaagg
    12421 gtcagctatt agcaaaaaag acaaaacatg tacttttcta ctaaaaagtt gatgtgatag
    12481 gcttctatct aaataactat taggagtttt tatcgtgaag caaaatgatg gacgtatcat
    12541 ttggaaaaat caaagtgagt taaaactgat tttaacaatt aatgaattca tcgaaaaaca
    12601 cggaattaca tctagccgtc aataccagaa aaagctatca gaaaatccaa attctgcacc
    12661 aagtatgtgg ttcattaaca aaaaatatgg atcatgggag aatttattga ttagtatagg
    12721 acgagaaaat actgggtatg gaaaatgggc tagaatgtca gaacaagaat tattggaaat
    12781 agtagaagcc tttattaagt gcgagaagat aacctctcaa cgaatgtacg aacaaaaatc
    12841 agtagggaag aatattcctt cacttagtac cattaaaaag atgttaggag atatacgtcc
    12901 actttttaaa gaaaaaaatg atggatctcg atttactgat ttcgaactgc tgttagaact
    12961 aaaaaatgaa ataatccgat taaaactaca ggatgattta tcaatgacaa agtttcgaaa
    13021 actagttcaa tccccaaaac taccatcagt tgatacaatt atgaaaagaa cgaacaaaaa
    13081 ttgggaggaa ttgatggcag agattggatt tgattatcga agaatcaaaa tttataagca
    13141 gagaaacaac ctatccaaga caaagaaaac taaatagtat cactctaaat cagacgttca
    13201 aaattaaaat ttggacgtct tttttattac ccaaaaacga aaggagattt attttatgaa
    13261 aaagccaaga tttaagaatt ggcgtttgct cgcaacactg agtctattat gccaaacaat
    13321 tggtggatcg cttggcccaa cgattgcctt tgctgatgaa attactcatc cacaaacggt
    13381 aactgtggaa ttggatttgg ctcaccagta tgctgtcgaa ggtacattta gcgatggtcg
    13441 tcccatgtca gaagttaccg ttccgcacta tgcagtttac aatggtgtga agcaagatat
    13501 tttctgtatt gagccagggg tgccgattta caatgagttc actcctggtt atgagaaaaa
    13561 tccattgcct gatatgtcgg agaaagcgaa attagtttcc gttttatgga aggaagctgg
    13621 tacggatgtg gatacacata ttgtggccca gaagatgatt tggcaagaag ttaatggcta
    13681 cacgcttcac tcaattaaac gttctgatgg tagtgcagta aatatcgcag ccattgagtc
    13741 aaaaatcaat caagccattg cggattatca aaagaaacca agcttccata actccacagt
    13801 gaaaaccgtg ttaggtcaat cgactactgt gacagatacg aacggcttga atttatcgga
    13861 gtttgatgaa gttgtcgaga atatcgcaaa tattgattat cgtgtgaacg gcaatcaact
    13921 agtgattact ccaaatgcca attcaaaaga gagtggtgaa ttaaccctaa agaagtcagc
    13981 taatacagga acaccggtag cttataaaat ggctggtcaa caaactttga tggcgggggc
    14041 aattgataag cccaatacct acacaatcaa aattgatgtg gaaactgagg gttctttgaa
    14101 gattaaaaaa gtcgataaag aaacaggaaa tgttgtaccg ggaaccgttt tccatttaga
    14161 ctttggaaaa acattacctg caaaagacgt gaccaccgat aaagaaggca ttgcgacatt
    14221 ggatgagatt cctcatggcg cgaaagtaac cattactgaa aaatcagtgc cagctcctta
    14281 tacgattgac actacaccaa tcaccgcaac cattaaatct ggtgagacta tttctgtgac
    14341 ttcaaaaaat actcgcgaaa aaggtcaaat cattttgaat aaaatggggg tggaaacagg
    14401 aactgatctt tggaacgaca aatactcatt agccggaaat acgtttgcca ttcgcaaaga
    14461 cagtccaacc ggtgaaattg tccaagaaat gaccacggat gaaaaaggtc atgcggaaac
    14521 accaaaagag attgccaatg cgttggaatt gggaacctat tatgtgaccg aaaccaaagc
    14581 tagtaatggc tttgtgaata cctttaaacc agtaaaagtc gaattgaact atgtcaatca
    14641 aaccgtggct cttgttacca gtaacgtaaa agggcaaaac caagaaatca ctggtgaaac
    14701 cactttgaca aaagaagaca aagataccgg taatgaaact caaggtaaag ctgagtttaa
    14761 aggagctgaa tatactctct ttactgcaaa agatggtcaa gcggtcaaat ggagtgaggc
    14821 ttttaaacca gagttagtga agggaacgaa agcttctgat gaaacggtga ctttgacttt
    14881 agatgaaaag aatcaagttg ctgttaaaca cttagcgatt aatgagtatt actggcaaga
    14941 aaccaaagca cctgaaggat ataccttgga tgaaacaaag taccctgttt ccatcaaaaa
    15001 ggtggatgat aacgaaaaaa atgccgtgat tacccgagat gttacggcaa aagaacaagt
    15061 cattcgcttt ggctttgatt tctttaaatt tgctggatca gctgctggga ctgccgaaac
    15121 tggcttcaat gatctgacct ttaaagtatc gccattggaa ggaaccagtg agattacaga
    15181 tgccgaagat gaagcaatta cagcttataa cgagcaacta ggttttgatg gctatggcaa
    15241 gtttgaaaat cttccttatg gggattattt acttgaagaa gtagaggctc cagaaggatt
    15301 tcaaaaaatt acgccattag aaatccgttc tacttttaaa gaaaataaag cggattacac
    15361 taagagtgaa tacattttta cgattaccga aaaaggacaa aaacaaccaa ttaaaacggt
    15421 gaccgttcct tacgagaaac tgacgaataa agcattttct gttagcttga atcgtttgat
    15481 gctctatgat ttacctgaag aagaagatag cttaacttct cttgggactt ggaaagacgg
    15541 aggcaaagaa ttgacagacc ttgattctac cgagctagtt gataaattga gctataactt
    15601 gcatgaaatc aaagaagact ggtatgtggt agcgcaagcc attgatgtgg aagcgacaaa
    15661 agctgcccaa gaaaaagatg aaaaagccaa accagtggta attgccgaaa caaccgcaac
    15721 gttggcgaat aaagagaaaa caggaacttg gaaaattttg cataaattaa ccgcagaaca
    15781 agttttggat aaaaccatcg tcttattcaa ctatgtgtat gaaaacaaga aaccctatga
    15841 agcaggtgat gatccagtag cgaaagatgc tagcttgaac aatcaagccc aaactgtcaa
    15901 ttgtacggtg gaacgccatg tttccatcca aacaaaagcc cacctagaag atggttcgca
    15961 aacctttact catggggatg tggtggatat gttcgatgat gtgtcgatta cccatgatgt
    16021 actggatggc tcaaaagaag cttttgaaac aattctgtat gcattacttc cggataatac
    16081 gaccaaagaa atttggaaat ctggcaaaat tgattatgaa gtgaatgaca aagaattcac
    16141 taaaaccgta cttgcggaaa aagtggacac aagtaaatat ccagaaggta caagttttac
    16201 cttcaaagaa attaactatg gcaaagacgg aaacatcaat gggaaacaca atgaggatct
    16261 aaaagaaaaa actcaaactt taacacctaa aaaagttccg acaacaccaa gtgcaccaga
    16321 acaaccggaa ataccaactg ttccaagtga ctctcaagaa tctagtccta cagtaaagac
    16381 attcccacaa acaggggaga aaaattctaa tgttctgctg tttattgggt ttaccttgat
    16441 ctttgcgact gctgggtatt atttctggaa tcgccgaaac taaggtgatg tgatgaaaca
    16501 actaaatagg aaaaaccaac gtgcgcgacc gcctcctaaa aaaagtggtc gcgctatttt
    16561 tgtatccaaa actaaaaatg aaaagaggaa aagaagatgg aattgaaatt tgttgtgcca
    16621 gatatggccg aaacctttgg aaagataagg tatgccggcg aaggcgaagt tttaactgaa
    16681 ggatatgggc ggaatactac agtaattggt cgtagttatc atttgtattc cagtaagcaa
    16741 cgagccgatg atattgaagt ggtggtagct gcggaagccg gcgaaaagga ttttgatcaa
    16801 gatcaaccac taaaagccgt gaatccccat ttggttgcca aaggctatga gattgagaat
    16861 cgtgggttta ccgactacgt gttgtatgtc gatgatttag tgaaagcata ggaggaaaag
    16921 aagatgagat taacagaagg tattgttgta gattcaggac taacgtttgg gaaattacga
    16981 ttttccgcat tacgccgaga agtacggaag caaaatgagg atggaacggt tagtaacgag
    17041 gtaaaagaac ggacttataa tttgaaatcc tctgcgcaag gtcgaatgat ccaagttagt
    17101 atcccagcaa atgtaccctt acgcgagttt gcctatgacg cagaggtaga gttggtcaat
    17161 ccaatcatgg atacggtcgc caattatgtt ttccgagaag gaactactgt caattggttc
    17221 attaaggccg atgatttagt cttgaaacga caaccagatc agggaaattc tacaaaccaa
    17281 aatgaaggaa agaaataggt gaagcgtatg gaaattaatg ttgaacatat tttagattgt
    17341 ctcgatcaat atggaaaagg tgagctaacg gaggaacaac tcactaaagc attaacctat
    17401 gatgaaaaaa tgtttctgat tatgcatcaa gggttactgg aagtaggtaa cgatgccgaa
    17461 gaagattttg atgtactgaa ttggttaggg gaagaagagt ccttctttat ggttatcgaa
    17521 gtaaatgaaa gtctatgtcg agaagccgaa accgtattgg aagaaattgg tgtggaaatg
    17581 ccggatgcca ttgaagtttt tttaaagcaa ctagtggaaa cgaaacaact ccctatagca
    17641 gtaaatgact agcataactg cttgaaattg gatcaatgaa atcctatgaa gcttttttcg
    17701 attggaggtg gaaaccataa aaatgtacaa agggcatcgt atacgggcag gagatcagca
    17761 cttggtttat cactttgttc ttggctggct actcgctctg ttcatcggtt ggatgagcgt
    17821 cttttatttt caagagctta gacaatttga tatttctaag ctatcacttt ctactataga
    17881 aatcgttcgt tctataaaag atttaatcta tttgctagga agtctcgttc tttcaggaag
    17941 cacgatgttg ctttatatac acttttttca ggatcattgg cgcagtttgt ggcatcgaca
    18001 aaagctagcc cggatgattc tagaaaatca ttggtacgaa gtaaaacaaa ctcagagtga
    18061 aggatttttc aaagacctca atagtagtcg aaccaaagag acaatcagtt acttccccaa
    18121 aatctattac cgtatgaagg atggtttact ttctattcgc gttcaaattt cactgggaaa
    18181 atatcaagat cagcttttaa agctggaaaa gaagttagaa agtggtctat attgtgaact
    18241 agtagaaaag gaactcaaag actcctatgt ggagtacact ttactgtatg atataattgc
    18301 caatcgaatt gggatagacg aagtagtggc agaaagtggg gctttgcgat tgatgaaaaa
    18361 tcaagtatgg gcttatgatt ctttacctca tatgttaatc gctggcggca caggtggtgg
    18421 gaagacttac tttttactca ctatcatcga ggctttatta aaatctgatg ccgagctttt
    18481 tgttcttgat cccaaaaatg cggacttagc cgatttaggt acggtaatgc ctcatgtcta
    18541 ttcccaaaag gaggaaattt ctgcttgtgt ggaagatttc tatgagcgca tgatgactcg
    18601 tagcaaggca atgaaagaaa tgcccaacta caaaacagga gagaattatg cgtttcttgg
    18661 acttccacca aactttttaa tctttgatga atacgtggtt tatatggaaa tgttaacgac
    18721 gaaggaaagt gcggtgattt tgaataagct caaacaaatc gtgatgttag gtcgtcaatc
    18781 tggtttcttt ctgattctag cttgtcaaag accggatgcg aaatatttag gagacgggat
    18841 tcgcgatcaa tttaatttct gggtggcttt gggtcgtatg agtgaactgg gatattccat
    18901 gatgtttggc gaggttgata aaaaattctt tatgaagcgt atcaaaggtc gaggctatgt
    18961 agatacggga gggagtgtga ttagtgagtt ttatacgcca cttgtaccaa aaggatatga
    19021 tttcttaagg gaaattagcg acattggtgg attaaacgtt aatacggaag gataaaataa
    19081 tagtatgtag cctaggagtg tcaagaatcc tgggcttttt ttacgaggtg atttatgaca
    19141 tttttagatt tattcgctgg gattggtggc ttttgcttag gtatggaaca agcgggtcac
    19201 cagtgtatcg gcttttgtga aattgatgat tttgctcgcc agagttataa ggcaatccat
    19261 gatacaagta aggaggtgga aatgcatgac atcacaagtg tatcagacga gtttattcaa
    19321 tctctcggac cagtggatat cctttgcggt ggatttccgt gccaagcttt ttcaattgcg
    19381 ggaaagcggc aaggattttc cgatactcga ggtactttat tctttgaaat cgctcggttc
    19441 gccgctattc tccaacctaa gtttttattc cttgagaacg tccggggatt actcaatcac
    19501 gaaggagggg ctacgttcga gacgatcctc cgaacgctgg atggattggg gtatgatgtg
    19561 gaatggcaag tgcttaactc aaaggcctac gttccccaaa accgtgagcg gatcttcctt
    19621 atcggacatt ctcgagacgc atgtaccgaa caagtatttc ctatcattgg atcgtctcca
    19681 acatctgatc aaaacatcag aaacttatta aatatcaacc cttccaatcg aggaatgggg
    19741 ggacaagtct atgggtcaga tgacgtagcg cctaccttga ccggtgatga aggaatcaaa
    19801 atcgcattgc cggtagatga tggaatttct gtagcgggta tgttactggg aaattttgaa
    19861 caaggcaatc gggtttatga gataactgga acagcgccta ctttgtcaac gaaacaaggc
    19921 ggaacgaaga tcatgcttcg gaaaaacgaa acagaatatc aagaggtgaa gccagggaac
    19981 agtgtgaacc tggcttttcc taattccaca agtcgtcgag gccgtctggg aaaacaaagt
    20041 gtgcatacct tattaacagg agaccaacaa gcagtcgtca cggatcagta tcaaattcga
    20101 aagttaacac ctagggagtg ctggcggctt cagggctttc cggactgggc ttttgatcga
    20161 gcttctcaag tcaattcaga tagtcagttg tataagcagg ctggaaattc tgtaacagtt
    20221 ccggtcattt ttgatattgc tagaagatta aaggaggtaa aaacagatga tttatgatga
    20281 gttgattggg gaatctactg ggtgattggc aagattcagt cagatccgga attggaagaa
    20341 gaactgtacc ggctcaattt tgatattcgc aaaaacgggg tgaaagtccc tgatgatccg
    20401 tatgtaattg atgaggaaac ggatgctcga atggagctga atcaagtcat cagtgaattg
    20461 gaacgaattg cggactttgc taaagaacct gatatccgcc aatacttgtt tgagataaaa
    20521 gcagagttgg aaattgaggg tattaccgcg gagtaacggc caaataaacc actgataggc
    20581 actgaccgcg ggggatttgc gaagcagaaa tcctccgtgg tgctgtgcct gtttatggac
    20641 ggcgttagcc ggccatgatg tctcaccccc cgtatctaac aggggggtac aaaaacacaa
    20701 aaaataagca agcaacgata aggaatggct gtcctatcaa ggataataag caatttgtgt
    20761 agatgttaac tgatttgaaa aagttaacac cttaaatttg gaggtgagaa cttggcacaa
    20821 aggaatttag attatcggct attaaaagac cggcgtaatg aatatggcgt ttcacaaaat
    20881 aaattagcta cgacttgtgg acttagtcgc ccgtacttaa atcagataga aaatggtggc
    20941 gtaaccgcat cgacaaaaac catgaggaaa atttttgacc aactggaaag tttcaatccc
    21001 gagttacctt taacgttact gtttgactat gtaaggattc gctttcccac aacggatgcg
    21061 aggaaaatca ttcaagagat tctccatttg aaatttgatt atatgctcca cgaagattac
    21121 gccttttact cctatcaaga acaatatgtt atgggagata ttgtggtgat gttgtcacat
    21181 gaagaagata aaggcgtcct tttagaatta aagggtcgtg gttgtcggca gtttgaaact
    21241 tttttactcg cccaaaagcg tagctggtat gactttttcg aagattgtct aaaagccggt
    21301 ggcgtgatga aacgcttaga cctggcaatc aatgatcgag ttggcctgtt agatattcct
    21361 gatttaacga aaaaatgtca gaaggaagaa tgtatctcct tgtttcgtac cttcaaaagc
    21421 tatcgttccg gggaactgtt gaaacctgat gataaagatg gtatgggcaa tactttgtat
    21481 attggcagtc ttaagagtga ggtctatttc tgtttgtacg aaaaagacta tgagcaatat
    21541 atcaagttag ggattccttt agaccagacc gaaacgaaaa atcgttttga aatccgactg
    21601 aaaaatgatc gagcttatca tgcaattcaa gatttgttaa aaggtcgcag tattgaaagt
    21661 accacatttt ccattattaa ccggtacttg cgatttgcgg ataaggtgga aggcaaaagg
    21721 cgaactaact ggcccttgaa tgaacaatgg gggcgcttca ttggacggaa tcagaaggaa
    21781 attcaattaa cttctgaacc gaagccttat accattgaac gaacgttgaa ctggcttgga
    21841 cgacaagtcg cccctacgtg gaagatggcc aaggagttgg atcggttgaa tcagacaacg
    21901 tacattcagg atatggtaaa taatactcga ctatcagacc ggcacaaaaa gattttggag
    21961 caacaaagta tggcaattga aaatttgatt atatgaaaag aggaatcgtg atggaaattg
    22021 tgaaagatat tttattggtg tttggtggca gtatgcttgg tgttaccgtg atgtgcttga
    22081 tgcatgcaag tgcagcggcg gaccgagcaa tggaaaagaa aggaaagtga tgaaatgaat
    22141 ttcggtcaaa atttgtatca atggttttta accaatgctc aatctttagt cttactagcg
    22201 attgtggtca ttggtttgtt tttaggattt aaacgcgagt tttccaaact gattggcttc
    22261 ttggtgattg ctttgattgc ggtaggtctg gtctttaatg cttctggtgt gaaggatgtc
    22321 ttactcaatt tgtttaaccg aattgtcggt gcctgagggg gaatcacaat ggaacagata
    22381 cgtgtttata ttgcgaacct tggaaaatac aatgaaggtg agttggtggg ggcatggttt
    22441 accccacccg tcgattttga tgaagtgaaa gaacggattg gattaaatga cgaatatgaa
    22501 gagtacgcaa ttcatgatta tgaactgcct tttgagattg atgagtatac accaattgag
    22561 gaaattaatc ggttgtgtgg tttagcggaa gagcttaaag gaacgccaat tggtgaggtc
    22621 gcttcagaga ttcaacatgc cttcttcaat tctttcgagg aaatggtaga gcatgtggat
    22681 gatatcatct attatcccga ttgtaatgac atgagtgatg tagcgtatta cttgattgta
    22741 gaagctggtg atttaggcga agtaccagaa cacttgaaga cgtatattga ttttgatgcg
    22801 tgtggtcgag acttggagat tggcgggaat tatttggtta cgagtcgagg ggtttttgaa
    22861 ctaatgtaaa tcatttgtta gaattgacca gaaatttcta tcgtactttt acttttctat
    22921 ttgatatact gaaatagaaa atatttgttg ataggggtgt tactaatggt tggcaataag
    22981 tggtacaaat gtgatcttca tattcattca actgcgagtc agtgctttca ggataaaaag
    23041 gttactgcta aggaatgggt agaagaatgt ttaaacaaag gactcgattg tgttgcatta
    23101 actgatcaca atacagggga aaatattgat gaatataaga aagtggctat cgaaaatggc
    23161 ttagattttt ttcctggagt ggaattaacg tgtggagaaa atggaactca tttactagtc
    23221 ctatttgata aaagtgcatg tacaaatgat atctctgatt tcttaaccaa gatgggtata
    23281 aaaagagagg attgggccag cgctgcagct tgctcaatga aatcagtttt agaagttatt
    23341 gattttgctg agcacgaggg cttgctcgtt attcctgcac atatagatga gtttaatgga
    23401 atttcttcag caaaatttgg aaatctagga aagatatttg aatcagaaaa tatatatgca
    23461 gtacagacgg ttcaaaaaga attttttgaa aatcgctcac aaatgattcc ctcatctaag
    23521 cgtgacacta tctatgat
//
LOCUS       DACEPD010000042        23181 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.043, whole genome shotgun sequence.
ACCESSION   DACEPD010000042 DACEPD010000000
VERSION     DACEPD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.043"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(540..1565)
                     /locus_tag="IW797_001534"
     CDS             complement(540..1565)
                     /locus_tag="IW797_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1224317.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1840..3525
                     /locus_tag="IW797_001535"
     CDS             1840..3525
                     /locus_tag="IW797_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ1224318.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3539..4822
                     /locus_tag="IW797_001536"
     CDS             3539..4822
                     /locus_tag="IW797_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1224319.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4892..5761
                     /locus_tag="IW797_001537"
     CDS             4892..5761
                     /locus_tag="IW797_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1224320.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5758..6594
                     /locus_tag="IW797_001538"
     CDS             5758..6594
                     /locus_tag="IW797_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1224321.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6620..7687
                     /locus_tag="IW797_001539"
     CDS             6620..7687
                     /locus_tag="IW797_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ1224322.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7684..8511
                     /locus_tag="IW797_001540"
     CDS             7684..8511
                     /locus_tag="IW797_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ1224323.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8556..9581
                     /locus_tag="IW797_001541"
     CDS             8556..9581
                     /locus_tag="IW797_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1224324.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9578..11761
                     /locus_tag="IW797_001542"
     CDS             9578..11761
                     /locus_tag="IW797_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ1224325.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11761..12891
                     /locus_tag="IW797_001543"
     CDS             11761..12891
                     /locus_tag="IW797_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1224326.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12888..13565
                     /gene="pgmB"
                     /locus_tag="IW797_001544"
     CDS             12888..13565
                     /gene="pgmB"
                     /locus_tag="IW797_001544"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ1224327.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13649..14176
                     /gene="msrA"
                     /locus_tag="IW797_001545"
     CDS             13649..14176
                     /gene="msrA"
                     /locus_tag="IW797_001545"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1224328.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14377..15063)
                     /locus_tag="IW797_001546"
     CDS             complement(14377..15063)
                     /locus_tag="IW797_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ1224329.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15143..16681
                     /locus_tag="IW797_001547"
     CDS             15143..16681
                     /locus_tag="IW797_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ1224330.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16801..17268
                     /locus_tag="IW797_001548"
     CDS             16801..17268
                     /locus_tag="IW797_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ1224331.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17787..18230
                     /gene="rplM"
                     /locus_tag="IW797_001549"
     CDS             17787..18230
                     /gene="rplM"
                     /locus_tag="IW797_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ1224332.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18246..18638
                     /gene="rpsI"
                     /locus_tag="IW797_001550"
     CDS             18246..18638
                     /gene="rpsI"
                     /locus_tag="IW797_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ1224333.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18727..19953)
                     /locus_tag="IW797_001551"
     CDS             complement(18727..19953)
                     /locus_tag="IW797_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1224334.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(20025..20183)
                     /locus_tag="IW797_001552"
     CDS             complement(20025..20183)
                     /locus_tag="IW797_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1224335.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            20871..21611
                     /locus_tag="IW797_001553"
     CDS             20871..21611
                     /locus_tag="IW797_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224336.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGETNYIESSKN"
     gene            complement(21703..22890)
                     /locus_tag="IW797_001554"
     CDS             complement(21703..22890)
                     /locus_tag="IW797_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1224337.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT         7516 a         4141 c         4789 g         6735 t
ORIGIN      
        1 tctaaaataa tttgtctaac tagaagaaaa gtgggaaagg atcttaacaa catcggttcg
       61 tatccaatta aggtatcgga acgatcatct tccaagagaa gtcaatcaac aaaagtaaag
      121 atagatcatt ttttgaatgc aaaataaatt gatgggaaga tagagcggtt atttttaata
      181 ttttgctagc tattattgaa taattcctcg aataaaacaa aaccagtaga catgtctgtt
      241 attctattta tgttttagag ataagtatcc ccaaataaca tggattgaaa taagggtaaa
      301 tgtgtttaaa aaaacgaact ataaaaactt atacacaacg tggttaaaaa tagttttttc
      361 ttccctgtgg aaaaaatagt ttaacacaaa cattatagtt atccacagct atctaaaaca
      421 gatggaaaat aagaaaaatt cgctaaaata ccgggagggg gtaacgaggg atgagtgatt
      481 gaaaaaagaa cagagtaact gacttatcct cagtgtttct gttcttttat aattctgttc
      541 tacatttctt gttgttggat cgaatctgtg agagtaatct cggttgaaag aatgattctt
      601 ttttcttcag ggtattggtg gtggattagt tggaataaaa gttctgtggc tttgattcct
      661 aactgatttg tcggaatgtc tatccgagtc aactgtggcg aaaataggga ggacaataag
      721 ggatcactga atcctgcgat cgaaagatca tcaggtatct gctttccaga ttgttctgcc
      781 actttgtaaa tactcaatga ttttaatgca tcggtagaaa aaatcgcagt tggtctttct
      841 cgtagcttca ttaaatcatt cattgctgcc atgctatctt ctaagcgaaa gccgccgtct
      901 ataatccatt cattacggat atctaggtgg tgatcaaata agcattgacg gtaaccattg
      961 agacgatcat tggatacata aacttctgtc ggagcatgga gacagccgat atgacggtgt
     1021 ccattcttga tcagtgtttc caccaaatcg taactatcct ggaaattatc cgtgtccact
     1081 gagtagatgt tcgtgtagtc gtttccgact tttccgatta ccacaatagg aatattgtat
     1141 tgatccaatt caaaaagaag tgtttcatcc atcgtagagc ttaaaataat aattcctcgg
     1201 atcatttttt cttggatctt tttcgcgcat ttttctagct cttcttctgg tgtttttgcc
     1261 gtttgtagaa tcaagtcgaa gttattttgt tcagaaacgg cagaaatgga ttgaataaca
     1321 tctgaaaaaa aaggattgcc ataggtcgta tgagaagtac gatgagataa tgctagaatc
     1381 gcgtcaaaac catttcctga cagtcctctt gcaattttgc tcggttcata attcaattct
     1441 ttgattgctt gaagtacact tgcttttgct ttatcagaga tatttggctg gttatttaat
     1501 actctggaga cagttgattt tgagacatta gcttttttcg caatgtcata aatagtaggt
     1561 ttcaatttta tatgcacctc ctttttggga gcgttttaga ataattccat agtacaatag
     1621 acattctttt ttgtctatga ttttgaaaat ttaggaatag cttgcatgat catgagagac
     1681 catatttttt taagaagcta ttaattattg atacataagc tttcttattt gtatttttgt
     1741 gtatatcaaa attatttaaa aaaatgctat tgacagtaag cgttttccat aatatgatca
     1801 aatcgttggg aacgctccca aaaaagaaag gggaatccga tgtatacaaa acttttacaa
     1861 gcgatttatc gcgactcaga tttgtctgag aaaaccgcct ctatgattga acaaaaagtc
     1921 agtgtttatc agcagaagaa aggccaggca ggcaaactgc attgggacga agaagatatt
     1981 ttcttgatta cttatgggga tcaatttttt gaagagcagc aaccaacttt gcgtacattt
     2041 aagaaatttt atcaaaagta tttggcagat acatttagag ttgtacattt cttgccattt
     2101 ttcccatata catctgatga tggtttctcc gttgtaaatt atcaggcaat caacgaccgt
     2161 ttgggcacat gggaagatat agaagaaatg agaagcgaaa cacggttaat gttcgacttt
     2221 gtctgcaatc atatgtctgc cgaaagtact tggtttcagg aatatttaaa cttgaatgat
     2281 acgtacaaag atttttttat tgatttgcca ctggatacag atacaagcag cgtgactaga
     2341 ccaaggacca gcccgttact tacaacgttc attgacaaaa acgggaaaga aaaacacgta
     2401 tggacgactt ttagcgaaga tcaagtagac ttgaatttta aaaatccaga agttttattg
     2461 aaaatgatcg atattctttt gttttactta gaaaaaggcg cttcatttat tcgcttggac
     2521 gctgtgggat ttttatggaa agaactagga acttcttgta tacacctgcc agaaacgcat
     2581 aaaatcatcc aattattccg tgcaatcgct gatgaagcag ctccaggaac ggtattgatc
     2641 actgagacaa atgtgccaca caaagataat atcagttatt ttgggaatgg cgagaatgaa
     2701 gcacagatgg tctatcagtt ttcgctacca cctttgaccc ttcaagcgat tcgttcgggt
     2761 aatacaagta agttgaaaga atggttaaaa accatcgatt tcgcatcgaa aaaaacaact
     2821 ttttttaatt ttttagcctc ccatgacggt atcggattga atccggtacg aggtattctg
     2881 ccggaaaaaa cgatccttga attagtggaa cagctgaaga aagagggtgc ccgagtaaac
     2941 tacaagaaaa acccagatgg aacgaccagt ccatatgaga taaacgctac gtatctagat
     3001 gcgttaagtt ctccaacaga tacgacggaa tggagaagca aacgcttttt agctgctcat
     3061 tcatttttgg taacgttacc aggcgtgccg gcaatttata ttcaaagtgt gctgggaagt
     3121 cgaaatgatt acaaaggcgt agaaaaaaca ggagagaatc gaaaaatcaa tcggcaaaaa
     3181 tttttgttag cagatattga gaaagagttg cagacagatc cattaagaac agcagtatat
     3241 ggtgaattga cccagatgct atctattcga aaaaaagaaa aagccttcca tccgaatagt
     3301 ttgatttact tactggactc agcagattct tttatcagtt ttattcgcga agcgaaggaa
     3361 gagaaagtct tagttatcca taatttttcc aatcaatcag caggttatct tttaccaaaa
     3421 ggagattacc tagacctgtt ttctggacaa attatccatt gcagtcaaga acgaaagttg
     3481 atattccagc cgtatgaata tatgtggctg aaaataataa aataagaggt ggaaaaaaat
     3541 gaaaaaaatt attgttacta cctgtctgct agctactgga tttcttctta ctgcttgtgg
     3601 tgaaagtcaa agctctaacg aatcacaaca gacaacgatt caattcatgc actcttctgt
     3661 cgaacaggaa cgattaagtg tgatcaatga actggttgct gattttgaaa aagaaaatcc
     3721 agacatcaaa atcaagcaag tgccggtaga agaatcagcg ttcaatacaa aaatcgttac
     3781 gttagctcgt tccggtaaat taccagaagt aatggaagtc agtcaggatt ttgctaaagt
     3841 gatggataaa gatgaactga ttgatcagac ggcagtccaa aacgttatcg aggaagctgg
     3901 agaagataat tattatgacg gagccaaaaa ccttgttcgt tcagaagatg gaaaaagcta
     3961 tattgctgca ccaattagcg gctgggttca aggtatttgg tatgacaaag aaaaattatc
     4021 agaagctggt ttttcagaac cagaaaattg ggatgatatt ttaaaaattg ctcaacattt
     4081 cacagataaa gagaataaac aatatggaat tgctatgcct actgctgaag gaacaatgag
     4141 tgagcaagcg ttctctcaat ttgctttgtc aaatggagca aatgttttgg atgatgaagg
     4201 cgaggtaacg atcgatacgg aaaagatgag agaagcattg tcgttttatc aaaacttatc
     4261 ccaatacacg atgccaggct ctaatgatgt gacagaaatc aaagatgctt tcatgaatgg
     4321 aacagcacca atggcaatct attcaactta cattttgcct tctgtttatg aagcggggaa
     4381 ctcagaaaat atcggttttg ctatcccaac acaaaaagat caagctgtct atggcacagt
     4441 atctggattg acgatttctt ctggtctaga cgagaaacaa aaagaggctg ctgaagcatt
     4501 cacagctttc ttatctcaac cgaaaaacat ggaaaagtgg gtcttgatgt ctcctggtgg
     4561 tgctcaacca gtgaataaac aagtagtcga actggatggt tacaaagaaa atgaagtcat
     4621 caagtctttt ggcgagctgc catctgagat tgctagtgct tttgatgaag tacaagtctt
     4681 tggactagtc ggggaaaaga atttcacgaa aatgggagat atcacaagct caggtgtgat
     4741 cggaaaagca gtgaaccaag taactgtcgg aaacgaagat gtcgatcaag ctttagctga
     4801 cgcacaaaaa aatacgaaat aacaagaagt tctgccaatg gtgttcgaaa aaagtagata
     4861 gctattggca gattatcagg gggaggtttc aatgaatcgt ccaaggaaac attctgactt
     4921 aaaattagcc atgggacttt tagcaccaag catgctttta ttagctgttt tagtgctgat
     4981 tccaatgctt tccaatattc agatcagttt tttcgatatg ccgctgaatc ctaactcaga
     5041 cgccgttttt atcggattaa gcaattatct tgaaatttta agtgatagtg aattttatcg
     5101 ttctttaggt ttaacagttg cttacacatt cttagttgta gcaggcagta cagctttagg
     5161 attagccgta gctattttgc ttaaccgcaa gttcagattt aggaaaacgg tgcgttcact
     5221 gattttatta agttatgtta cgccatctat ttctttgatt tttgcttgga aatatatgtt
     5281 caataatagt tatggaatcg tcaatgatct gttagtagat aggttgcagc ttttcaacga
     5341 agcaccgctg tggttcgacc agccagttgc tagcttcttt atggtcgttt tgtttgctat
     5401 ctggagatac tatccgtatg cgtttatttc atttttggct attttgcaaa cagtcgacaa
     5461 aacgctttat gaagcagctg aaatggacgg tgcaaatgct tggcagaagt tcaagatcgt
     5521 gacactgccg gcaatcaaac cagtactagc aactgtgatt gttttacgat ctatctggat
     5581 gttctatatg tttacagatg tctatctact gacgaataaa gtcaatatct taggcgttta
     5641 tttgtatcag acagcctttg cgttcaatga tcttggaaaa gcagcagcta tatcgattct
     5701 attgttcatc attattgcag cggtcgtctt gctctcacga aagaaggtgg acttgaaatg
     5761 aaaaataaaa agagcaggaa aatcttattt tacgcgacga ttttgttgct attagctgtc
     5821 aatttgtttc catttttgat catgctgatg acatcattca aggattcaca agaagcaatt
     5881 gcaacgacac aaactatttt tccgaaagaa tggacttttg aacattatca ggatattttc
     5941 aatgctgaga tttttccatt catcatgtat ttcaaaaata gtttagtgat ctccttgata
     6001 gcctccgtaa ctgcagtagt gattgggatc agcggggcat acgctttgtc aaaactacgc
     6061 ttcaaagctc gctcgaaaat caatgctagc ttttacaccg tttatatgtt ttccggtatt
     6121 ttgatcgttg ttccattatt taaaatcatt tccggtctag gtttgtatga taccaataca
     6181 gcactgatta tcacgatggt cgttcaaaca cttcctactg cgatattcat gctaaaaagt
     6241 tattttgaca cgattccgac tgatattgaa gaagcagcaa tgatcgatgg tcttagccgg
     6301 atccagatca ttattcggat cattatacca ttggctattt ctggaatcat ttctgtcttc
     6361 gtttattgct ttatggtttc gtggaatgat tatttatttg cttcgatttt cctgtcgacc
     6421 tccgaaaaat ttactttacc aatcggtttg aacacattat tcagtacacc agactacatt
     6481 tggggacgaa tgatggccgc ttcattggtg accgctttac cagtcatcat tatgtatgcg
     6541 ttatctgaac gatttattaa aggtgattta gtcgaaggcg gcgtaaaagg ataaaaagaa
     6601 aagaaagagg aaagaaaaga tgaaaaaatt agttgcatta gaaccaagaa tagccggatt
     6661 caaagattac gaagaccgtg agatcgaaaa gaacgaagta cgtatccgag taattttcgc
     6721 tactccaaaa ccaggaacag aaattgtcga ctttagagga ttaagtccat ttatcgatga
     6781 agaattttca gaagaatggc gcctattctt gccaagagaa gaaggaagcc aacgcggtgt
     6841 ggtatttggc gaatttcaat taggaaatat gattgtcggt acgatcgaag aaacgggcag
     6901 tttggtgaca gagtatcaaa aaggagagtt agtctgcact tatggaccca ttcgtgaaac
     6961 gctgattaca aacggtgttg ataattataa actacgtaaa cttgatcggt tggatcaatg
     7021 gaaaaatgca gtatgttacg atcctgctca attcgcttta ggtggtatcc gggaagcaca
     7081 tgttcgagca ggtgatgctg tagcaatctt cggtttaggc gctatcgggc aaattgcgat
     7141 tcaattagct aaaaaagcag gtgctactac catcgttggg atcgatccca tagcgcagcg
     7201 tagagaatta gcagaaaaac acggtgccga ttacacgcta gatcctatga aagcagatgt
     7261 cggttttcgt ctaaaggaat tgacagataa acgtggagta gattcagtta ttgaaacgag
     7321 tggcagtgct gccgcattac aagcttcttt aatagggatc ggctatggcg ggatcatttc
     7381 ttatgttgcc tttgctaaac cttttccagc tgggtttaat cttggtcgcg aagcacattt
     7441 caataatgca aaaatcgtct ttgctcgagc atctagtgaa ccaaacccag attacccaag
     7501 atggaaccgt aaacgaatcg aggacacatg ttgggaatta ttgatgaacg gttacttgga
     7561 ttgtgaagaa atcattgatc cagttgtgcc atttgcagaa agcgcagaag cttacatgcg
     7621 ttatgtagac cagcacccag aagaaagtat caaaatgggc gttcaagtcc gaggaggaga
     7681 acaatgaaat tcggtacgca agatcaagca tttttcccaa gagacatttt agaaaaattt
     7741 agatttatca aagactgtgg atttgaagaa tacgaaatcg acggtaagtt gttaatagaa
     7801 aatatcgaca cagtcaaaca ggcaatcaaa gaaacaggga tcccagtagt gacagcttgt
     7861 aacggctacg atgggtggat cggtgacttt atcgaagaac ggcgtcttaa tggtgtacgt
     7921 caaattgctg agattttgcg tgcactaagc gaggttggag gcaaaggaat cgttatccca
     7981 gcagcttggg gaatgttcac ttatcgatta ccgccgatgg tttcaccccg aagcaaagaa
     8041 ggagactata aagcagtcag cgaatcactt cgttatttag ataaagtagc cgaagaaaca
     8101 ggtacggtta tttatttgga gcctttgaac cgataccaag accatatgat caatttgtta
     8161 gcggatgcgc gtcattacat tgactctaat caattgaaac atgtgcagat catcgctgat
     8221 ttttatcata tgaatattga agaagatgat atttcaaaag cattgactac gcaaaaagag
     8281 tatgtgggtc atatccattt agcagataat catcgctacc aaccaggatc tggcgcaatt
     8341 gattttactc gccattttca aacgctactt caaaatgagt atgaaggtta tatggtatat
     8401 gaatgtcgtt tgcgaggaga aaatgaagtg aaaatttatc aggaatcatt gcaatacttg
     8461 aaaaactgtc tggaaacagc gcgtcattta aatgatttag agaaagaata gagaaagaat
     8521 aacaataagt aaataagcaa aggagagaga aataaatgaa gaaagtagca atcataggcg
     8581 ctggacaagt agcagaaaaa gtacatgctg cttattatca aaaaagatca gatgtcttag
     8641 aattggccgc tgtggttgat ccagtcagcc agcgagcaga agacttttgc cgacgtacag
     8701 gattttctca tgcgtataca tcagttacac aaatgctgaa tgagatttct cccgatatcg
     8761 tgagtgtctg tacacccaat cgctttcact tcgaacatgt attggctgcc ctcgaagcta
     8821 atgcttcagt gatgtgcgag aaaccaccag ctatgacagc tgatcaagca agaaagatgg
     8881 cagagacagc cgataaaaac gggcagattt tggcttatgg attccagcat cgtttttctg
     8941 acgaagcaac aatgatcaag aatcatcttg atgttctcgg ggatatttat tacgtagaag
     9001 cgaatgcgct tcgtcggtct ggtgttccag gctggggaac gtttatcgac aaagaacttc
     9061 aaggaggcgg gcctttgatc gattatggca ttcacatgct tgatacagcg atgtatatta
     9121 ctggatttcc taaagtaaaa agcgttcggg cgtacagctt ccaaaaaatt ggtacgaaaa
     9181 aaaattcagg gaaattcggg aaatgggatc caagcaaatt cacagtagaa gatgcaattt
     9241 ttggcacact ggaattagaa aacggcaaga tcatccaact gaatacttcg tttgctttga
     9301 atatcagaga agacaaactg aatgtggctt tttgtggcga tcaggcaggt gctaatctgt
     9361 atccaggcga actattcgaa gatgtagatg gaaagctggt ttccttacaa aagaccacgc
     9421 cagttaatga cccacatgaa ctaagtatgg ctcattttgt gaatcgggcg ctcggtgacc
     9481 agactcagat gattgcagat ggctggcaag gatatgtcat ccagaatctg gtggaaaatt
     9541 tatatcgttc ggcagaaaca ggagaagtta ttaccttatg aaagtagaag aaaaacagtt
     9601 tgatttagag tatatcaata agtatgcgag tcagatgacc attggaaatg gattttttgg
     9661 tatcagagga agccaggagg aagactatcg gaatcaagta aggggaatgt ttgcagctgg
     9721 tgtatataac cgacctttag gagcagaatc agccgagtta gtgaatctcc cagatgtcat
     9781 cagatgggaa ataaaaatcg acggtgaaca tttttctctt gagacgatga aagttcttgc
     9841 atacgagcgg gcactagatt tagaaatggg agagcttaaa cgctatgcgc tcattgaaac
     9901 tgctcaagga aaaaaagtgg aagtcattac ttgtcgtgtt gctgatcaaa ataatctcca
     9961 tcaggcagcc ctgaaagtgg tcatcaagcc gttgaccgaa gactgccaag tagatgtgaa
    10021 aacgggtatc gatggacagc aaaccaattt tggtacacag cagttggttg aaaaagaact
    10081 gcgagtattt ggtgacgatt tgatcagtgc atcctaccag acaacagaaa gtaaaattga
    10141 tattgggatt gcagctaaat tcaataaagc aggtgtattc caagcaaaaa atcgccggat
    10201 caccgcttcc ttttcagaaa agatcagaaa aaatacgtcc tttacgctgg agaagatcat
    10261 cgtgctctct acctctcttc agcaaacaga cccgtttaag aaaagtattg cccaagccca
    10321 gaaaagcgtt agttatacag aaattttaga gcagtcgatg tcttattgga cagatttttg
    10381 gcatcagcat cggataacga tccaaggcga ttcttctttc gatcagttag cgatggactt
    10441 cgctgcttac catttacaga ttatgactcc ccgcaatgat ccaagtgtta gtgtaggggc
    10501 gaagggacta actggcgaag gttataaagg gcatgttttc tgggatacgg aaatttttat
    10561 cctgcctttc tttttatatc agtttcctga agtagcaaag aatttgctgc tgtaccgtta
    10621 tgatcgatta gaaggcgcac ggcaaaaagc aagagattat cactatcaag gtgcactttt
    10681 cccatgggaa agcgcacaca ctggtcagga agagacaccc aaatttgctg cattgaatat
    10741 caaaaccgga acaagacaag aagttgcttc agctgccgca gaacatcata tcagtgcaga
    10801 ccttgcttat gccgttcaag agtatgtcca agcaacagag gatcaatcgt ttttagaaac
    10861 aaagggaaaa acgctcattc atgaaacagc agctttttgg ctgagcagag ctgtaaaagt
    10921 cgatgatcat ttagagatac acagagtcat tggtccagat gaatacacag aatatatcga
    10981 caataatgca tatacgaact atctggcgca ttataatgtg gccttagctg ttaaatatca
    11041 agctggcgat ccgctttttt tgaaagaatg tcaaaatttt ttagataagt tatatttgcc
    11101 agtgcctaac aaagaacagc tgattccgca agatgacacg tatctcagta agccagttat
    11161 tgatttgacc acttacaaag aacgtcctgg cagccagtct attttacttg attatgcccg
    11221 tagcgaagta aatgaactcc aagtcagcaa gcaagcagat cttgtcatgc tgttatactt
    11281 attacctgag tatttttctc aaccaatcgt ttctaagaat ttgcattact atgaaaacaa
    11341 aacaatccac gactcatcgt tgagtaaagc gattcacgcc atagaggctt gccgtatcca
    11401 agaaatggaa tgggcgtatc atttattcca agaagcgtgc ctgattgatt taggaccaga
    11461 tccgcattca tcagatgacg gaattcatgc tgcttcatta ggagcactat ggctagcagt
    11521 tatatttggt ttttcaggta ttacagtaac gaatggaact ctttctattt ctccaagact
    11581 tccttcagca tgggaaaaaa tttcttttcc ttttatttgg aaaaatcaca ggttgctatt
    11641 tgttttgaca aaaaaacaaa tagtaataaa aaaagagacg aaagaaacat tacctatttt
    11701 tgtcaatgga gaccaatacc agctggaaaa agaactgatc ttggaacgga ggggaaaata
    11761 atgcgtattc tgattgcgcc agattctttc aaagaaagtc tgacagcctt agaggcagca
    11821 caagccattc aatcaggttt tcaagaagtt tttgttcagg ctgaatatga tctgctacct
    11881 ataggcgatg gaggagaagg aacggtttca tcattagcag acagtttaag gttgaagcgg
    11941 caaacagcag tcgttactaa tgcatttggc aaaaacaaag aaatccagta cgcttcagac
    12001 ggtcaaactg cgatttttga aatggcagaa atcgtcggat tgaaagatgt cccactaaac
    12061 caacaacagc cacttaggtt atccacgcga ggagtaggag aactgatacg acaattgttg
    12121 caacaaggtg ttcgacacat catcatcggc gttggcggaa gtagtaccaa cgatggcggc
    12181 atcggcatgg cagaaggtct aggttatgct ttttacgatc aagataatca aaaagtagaa
    12241 gcactgggag aaaatctagg taaaattgct acagttgata aaacgaacgt gcctattgag
    12301 ctgattaaag ccaaaatcac tgtcattact gatgttacta atcttttatg cggaaaagaa
    12361 ggcgcttctt ttgttttcgg accacaaaaa ggcttgaaag aaaatgaatt agcagtggct
    12421 gaccaagcca tgcatgattt ttatatgaga tttgcccctc aagtaatcga ggcatcgggc
    12481 agtagtgctg gtggtggaat gggtgcagga ctgttagcat ttgcttcagg gaaaatagtc
    12541 tctggcatcg actacgtatt ggatttactg gagttcgata aacgggtaca gcaagcagac
    12601 ctcgtaatcg taggcgaagg acgtctagat ggacaaagtc taaaaggaaa agcgccagtt
    12661 gggattgcta gaagagtacc agagggcgtt cctgtgatag ctatttgcgg aagtgttggt
    12721 aaaggaagcg aaaaagcagc agatgttggc atttcggccg tttttccgat ccttcctgca
    12781 gctgtgcctt tacaagaagc tttacagcaa gccactgata atcttcatcg cacagctaaa
    12841 aacatcgcat ctttacttca agtcgttcat tttcaaggag gtaatcgatg aaaatcaaag
    12901 cagcactatt cgatttagat ggcgttcttg tagacacagc tcgttatcat tacgaagcgt
    12961 ggctggtttt agctaaccag ctgtccattc cattcactga gaaagaaaac gagcaactga
    13021 aaggaatatc cagaaccgag tcactagagc gattgctgtc atttggaaaa atggaacaga
    13081 aattctcaga aaaagagaaa tcagcctttg ctgagcaaaa aaacaatcta tatcttcaag
    13141 cgattcaaaa aatggatgaa acatccgttt tgcctggtgc aatagctgtt cttgagtact
    13201 tgaaaaaaac aaacatcaag atcggattgg gatcagcaag caaaaatgca cgcctcattt
    13261 tagaaaaaac gaatttgaca agttattttg atgtccttat tgacggcacg caagtatcaa
    13321 aagcaaaacc tgatccagaa gtttttctca aaggagcaca gcaattgaat gtaccgccaa
    13381 atgcatgctt ggtcattgaa gattcagaag ctggttgtca agcagctttg gctggtaaca
    13441 tgcatgtatt gggaataggt gaaaacataa atcttccttc tgctgaatat gtgattcctg
    13501 atttaaccgt ttttgatcaa gtacgttcgt tttggcattt atcagaagcc gtatcacgta
    13561 tataatagtt tccgtttttt ctgtcacaag caaaacgtta tcctcctttt ggaaatcaag
    13621 gtatgatgag aaaaaagggg gattttatat gacaaaaaaa gcagttttcg caggcggatg
    13681 cttttggtgt atggtcaaac catttgaaga gcagccaggg atcatttctg tgacatccgg
    13741 ttatactggc gggcatgtac caaatccaac ttatgaacaa gtaacaacag gcacaacagg
    13801 gcatacagaa gcagtagaga tcgaatatga tccagaagtt atttcttatg atcagttggt
    13861 ggaaatctat tggcagcaaa ccgatccgac tgacgcatta ggacagttcg ctgatcgcgg
    13921 tgattcttat cgtccagtga tctactataa tgatgaagaa gaacgaaata ttgcagagcg
    13981 ttcaaaacaa gcattgcaag acagcgggcg attcgataag ccaatcgtga caaaaatcga
    14041 accaagaagc gaattttatc ctgcagaaga ttaccatcag gattattaca agaaaaacaa
    14101 gctccattac aatctttatc gagaaggttc tggacgtgca ggtttccttc gtcgacattg
    14161 gggcgtcaag aaataagaag ccgaaaaagc tacttatgga acaccgtata tcagaggttg
    14221 taaaaccagc attttgagct aatactgagg gtcagctgat tgaacaccat ttagtggaga
    14281 ggttgtgaac ctgtcgccta gctccaagtc acaaggattt ggtaagagaa cactatatat
    14341 cagaggttgt gacaagtcgt ttgtcacaac cttttttcaa tacctaagat gaaggacaat
    14401 cagcaacgca ctgctcaaaa aaggaatgaa aggcagcttt tccaactttt ttttatgaca
    14461 gataaagtaa acagaaaaaa cggatattcc aaacagacta gcaagaaaca agatctgaag
    14521 caactcttct agagacaagc caccactcca agaaagcatc aacagagtat cgccagctcc
    14581 gagataggat tgaccatagc gaaggcacac accgcctacg ataaacgtta acagcacact
    14641 ttgccattgg aagacgccgg cctgcaacca aaaaatccat aaaattgccc aagaacagaa
    14701 tagcgtgtga gaatggacca tgaagtaaaa taaatccatc aacgaaaata gcaaagccga
    14761 aagcatccat atgaacgtcg aaaaatttct tgcggaagaa aaagtaaaga accaggcaaa
    14821 aagaccacca cataaggctt ctgccaacaa atagtagaag ggcaaccgac acctgcaata
    14881 gcggcagcga aatcgttgga cgatgatcga gagaatcgga atcaattcat accaagaaag
    14941 cgaacgacag catttgacac aatgagatct aggataaata atagagtgac ctaacggaat
    15001 ccgctgggcg gttaaacata aaaatgatcc gatacagcaa ccaatgataa aataaagtaa
    15061 cacgccatct cccccttttc aaaaccaaga tacgcaagct taaactaaat ttttgtacat
    15121 acataaaatt tgaggtgaaa aaatgaacgt ttcaatcgga ttagatatcg gaaccacaca
    15181 aaccaaagca gttgctttca cagatgaagc ccaagaagca gcatccgctt attttcgcta
    15241 ccctttgatc caagaaacac aagggatggc agaacaagat caggaattga tctttcaagg
    15301 tgtctgcacc gttataaaag aagtaattaa acagttgcct tcagacacaa tcattcaatt
    15361 tgtctccttc tcctctgcga tgcatggcct gatattgttg gatgaagaga aaacaccgtt
    15421 atctcgaatg atcacttggg cagataaccg agcagcggac gaagcagaaa aactgaaata
    15481 taccgtgctt ggcaaagaat tttatcggca gactgggacg ccgattcatc caatgacacc
    15541 attgaacaaa atcaagtggc tgcaaaacag ccaacctgaa ttattcaaaa aaactgctta
    15601 ttttgtcggt atcaaagact atatctttta tagattgttt ggcgaactga tcagcgacta
    15661 cggaacagct tcaggaactg gatattttga tatccatgaa ttccaatggg cagaagaagt
    15721 gcttgactac ttgaacattc gaatagagca attgccgcaa gtctttcctt ccacctatca
    15781 agtaacaggt ctaccatcaa aaacagcagc agaactaggt ttgccaaatg atatatcatt
    15841 tgttttggga ggggcagacg ggccgctttc taatctaggg ttaggtgctt ttggtgatac
    15901 gatcgccgct ttgaccgtag gaacgagcgg agcacttcgt tttattacta ctcagcctca
    15961 gttccatcca gaaatggaga cgttttgtta tgttctggat cagaaacatt gggtagtggg
    16021 aggagcgact agcaatggtg ctggcatctt tgattgggca gggcatacat ttttacagga
    16081 agtcgttcaa tctgctaaag aaaaaggaga aaatccatat gatgcactgc tttcggctat
    16141 cgcttttgtg ccagcgggtg ccaatggtct gatttttcat ccctatttac ttggtgagcg
    16201 agcaccgctt tgggacgcag atgcgtttgg cagttttatc ggtttacagc ggcaacatac
    16261 agaaaaagaa atgatgaagg cgattctaga agggatctgt ctcaaccttt atcggatttt
    16321 aaaaggagtt tgccaggaaa atcagctcac agagatccgt gctacaggcg ggtttgctca
    16381 atctccgatt tttcgtcaga tgatggctga tgtactgggt tacccgcttg ttttccctac
    16441 tgtcacagaa gcatccgcat taggagctgt gattttaggc tggagaagca tcggaaagat
    16501 ccaaagtatc agtgaagcac aggaattgat agaattgaaa gagcaagttg gagtacaaga
    16561 aactgatcat aaaaaatatc agaaaatcta tccattattt gtttcgacac aaaagcattt
    16621 agcacagact tatcagcaat ttgctcaact gcgagaagaa ttagctgatt ctgaaaaatg
    16681 attggcttta cggataaaaa agcgaactga aaatcgttct caggtaaatg attttgttgt
    16741 acttttcatt gaaagtctac tattatttta agtgtaagaa aatgttagga ggcaagaaag
    16801 atgaaattcc aacaaaccaa agaagtatta aatcaattag ttgcagatct aagccaaatg
    16861 tctgttgttg ttcaccaaac acattggtac atgagaggac caggtttcct tacattgcac
    16921 ccaatgatgg atgagttcat ggatgatttg aacgctcagt tagatgaagt atccgaacgc
    16981 ttgatcacat tagacggttc accttattct acattgcgtg aatttgcaga taacacaaaa
    17041 atccctgatg aaattggccg ctgggatcgt actattccag aacgtttgga aacacttgtt
    17101 gcaggctatc gttatctagc agatctttac caaaaaggaa tcgaagtttc tggagaagaa
    17161 ggcgacgatc caacacaaga tatctttatc gaattcaaaa cagccacaga aaaacgtatc
    17221 tggatgatcc aagctcattt gggcaaagcg ccagagatcg acgcgtaaaa actgttgttg
    17281 aacttgaccg aaaaagaggt tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa
    17341 cgtcaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagctgatt
    17401 gaacaccgtt taccaagagg ttgtaagcct gccgttcagc tccgagtcac aagaaacaat
    17461 tttaaaaaac agcttcttat gttttatgga tattgtgact tgtgaccgag gagttggcag
    17521 gagaacaccg tttattcaaa ataaggggtt gtgacaagac ttttgtcaca gcctcttttt
    17581 gtttttcatt ttgacaggaa acgacccttt tatataaaag aaagaaaaga agtaaagttt
    17641 catataaatt tttcaatcaa gcagaaaaac gttgacattg actagattag cgggtaatat
    17701 gttaaatggt attgtttgcc ccacaaagag ccccggaaac tcgaagtgta tgtcaaacat
    17761 cagaacttaa attggaggaa aataacgtgc gtacaacata tatggccaaa ccaggcgaag
    17821 tagaacgtaa atggtatgta gtagacgcaa ctgatgttcc attgggacgt ctttcaacag
    17881 ttgttgcatc tgtactacgt ggtaaaaata aaccaacttt cacacctcat ttggatactg
    17941 gcgatttcgt catcgtcatc aatgcagaca aagtgaaatt aacaggtaaa aaagcgactg
    18001 acaaaattta ttaccgtcac agcaactacc caggaggatt gaaatcaatc actgctggcg
    18061 aattgcgtgc taaaaattct cgtcgtttga tcgaaacttc tgtaaaaggt atgcttccta
    18121 aaaacacttt aggccgcaaa caatttacaa aattgaacgt atacggtgga gcagaacatc
    18181 cacatgcagc acaacaacca gaagttttag acatcacaaa cttaatctaa taggagggaa
    18241 tttcattggc acaagttcaa tatatcggca caggccgtcg taaaaacgca gttgctcgcg
    18301 tacgtctagt accaggaact ggcaaaatta ctgtaaataa aaaagatgtt gaagaataca
    18361 ttccacatgc tgacttgcgt gaagtaatca atcaaccttt cgctgttact gaaacaaaag
    18421 gcgcatatga cgtatttgtt aacgtaaacg gcggaggata cgctggacaa tcaggagcta
    18481 tccgtcatgg tatcgctcgt gcattgctag aagttgatcc tgacttccgt ttagctctta
    18541 aacgtgctgg cttacttact cgtgacgcac gtatggtcga acgtaaaaaa ccaggtctca
    18601 aaaaggctcg taaagcatct caattctcta aacgttaata ttatttatat atcaatgttt
    18661 caaggcactt ttcaattgtt tgaaaagtgt ctttttcttg tttaagaaca catttaagaa
    18721 cacatactaa tcacatttat tcaatcctct ctgaaaggct tcaaaaacag ttttatctac
    18781 tacatctggt gtatttacgt atattctcgt tgtagaaata tctgagtgtg ttaaagcact
    18841 agatatttga ttcatatttg ctccgccttg tctagctata gttgcatagg tatgacgaag
    18901 cttatgtgga gataaatgag ccaattcagg atgtcgtctt ttaattgaat tgattcgata
    18961 gtttaggtaa tcagcgtgaa cagggcaatt tacttctccg gatggtttag tataggtaaa
    19021 gagatactga tctggtgttt gattaatttt taatctcaac aattgctcag cttgcactga
    19081 tttccatttg cttagtaatg ccattacaat ttcgggtact tgaattacgg tatcctttcg
    19141 accttttgta aatttctttt tttgatattt atccaaagca tgtttaaggc gaaccgtttg
    19201 tttctcaaag tcgatatatt tccattgcaa agcatacgtc tcactttttc tatcaccaag
    19261 atatagggta agcatgaaaa gagtatagtc atgaagagta agcaagtgat tattcaagtc
    19321 agtgtttact gcttctatcc aagcaataag ctcttctgac gataatgctt gattaccttc
    19381 ccgtatcctt ctttcttcta aagcaatttt tttaggtgca gtgatacatt gaataatttt
    19441 ggtagttcgg ttaaattcaa tataatttag tatttctgca atatcaaaca tagaacggac
    19501 atatcctttg ataattttta tatttgcgta ttctttcgat ttttttgtta attcatctga
    19561 tattatctca gtatttgaat ttagatagtt cattgcatac tcaccaaaca ttggaaggat
    19621 gtgcaatcgg aatatatctt tagtattact aattgttacg taacttggag ctctttttga
    19681 tctagtagtc tgacctagtt catatctagg aagccatttt gattcataga attccttaaa
    19741 ttttattttt ccttttaatt ctagtgaatt tgcattctcc tctcttaaaa ctgtttcaat
    19801 tttccttttc attgcatttt cggctttaat agccaattgc ctagttggaa atactttttg
    19861 atacttttct gatgtaattc cagtgatttc tctgaatttt tttggatacc tcagattagt
    19921 tttatatttc ccatttgaaa gttttgtagg caaaattcct cactccctta aaaagtaagg
    19981 cagtatattt cgctattctt agtatatgcg gcgagaagaa attttcaaat tgttttaaat
    20041 gtattcataa aatctttgat atcctctatg tcaaagcgaa ctataccgtc aattctgatt
    20101 actggaaaat tatatatacg aatccactta tctaaagtat tattatcaat tcctagaaac
    20161 tcacaggttt gttttttatt caagtagact ctcaatttta taaaatcatc tcctaaaaaa
    20221 gattagaatt tttgaattgc tttgggtaag cacataggaa tttcacctct ccccagttta
    20281 cggcgagcct tacggaagta tcattatttg ctgctattgt catggcattt tgttttccca
    20341 gaacaaaaag aaatctctaa gtatttgtcg ctaatcaggt catggcgtac ttgagtttct
    20401 agctcgaata gcagggatcg ttattcaaaa attgtattta attgtcaatg atcaaaaata
    20461 ggttttttcc tattcactat taataacgat aaacatgtat aaaaagacac actacgaaaa
    20521 aagttaaatg gagtttactg attttcatat ttgtatatgg aacagacata gtcttgaata
    20581 atttttataa atgttttgat gcttatcaac ttattcaacc cgctatccac tttaagtata
    20641 atttttccat acgccaataa agtagtttta ggagcatgtt ctcatttgag aacgctcctt
    20701 ttttatatat tttgaaagct acttactata cgcgtagttt caaatagttt tgtcattcta
    20761 atcttcagca acagaaaaaa atctaaatta taaaatttag ggttgactaa aatataattt
    20821 ttgtatacca tatgatcgtg ataatcatta tcatttagaa ggagaaagtt atgttagaag
    20881 tgaaaaagct taatgtttgt tataacgatt ttttagcatt gaacgagatt tctttaaaaa
    20941 tacaagaagg tgctttaaca ggtattattg gtccaaatgg tgctggaaaa tctacattgc
    21001 ttaaagcaat gttaaatatt atccctcatc agggagaggt ctccattaat aatgaagata
    21061 tcaataaaaa actggtgaag attgcatatg tagagcaaaa agctgatatt gattttactt
    21121 ttccaataaa agtaaaagaa tgtgtatcta tgggaacata tgccggaatg aaagtttttc
    21181 aaagaattaa aaatgcagaa tggcaaagag tttcaaaggc tttggaaaag gtcgatatgg
    21241 gaaaatattc taatcatcaa ataggagaat tatctggagg acaatttcaa cgtgtacttt
    21301 tagcaagatg tcttgctcaa aatgcggatt tcatattttt agatgaacca tttgtaggga
    21361 tagacttagt tagtgaacga ataattatgg atacgttaaa agaattgaaa catcagggaa
    21421 aaacagtgct aattgtgcat catgatttaa gcaaggtaaa gaagtatttt gataatatta
    21481 ttcttttgaa tcgaaatcta attgctcacg gaagtgtaga atctgtattt aatgaagaga
    21541 atttaaaaaa agcctatgga gatactatat ttattggaga gactaactat atagagtcaa
    21601 gtaaaaatta gccaaatcaa aaaatatttt ataacagata attccgaaaa aagggtacaa
    21661 caaataagct tcttaccatt agtttttttg aatgttcaat agctaatcag aaacaattat
    21721 ataaggctcg tttttagtca aaatagcatg aatcacgtaa caaagcttac gagaaaccgc
    21781 acctaaggca gttagatgat gttttccttc ggaaactttt ttctggtaaa acgcactaaa
    21841 aacaggatca cattgagaag ctactaaagc cgccgaaaac aaggcttttc gcaaatatgg
    21901 agaccctcgt ttactcataa cattatgcgt tgcctcatat tgccctgatt gtgtcacgga
    21961 agcgtctatc ccagcgtaag caacgagttt tttcgggtta gaaaattttt gaatatctcc
    22021 gatctcacca agaatagtcg caccgtttat aggaccaatt ccaggaattg tgagaataac
    22081 agaatttaaa gtgtccatta tgtgcttgat ttctctttcc gtttccttta tttggctttc
    22141 aatgaatttt aattgttcaa tcattgaacg tagttggaag gtgaacgctt cttgagcaaa
    22201 ttttactcca aaggagttag aagctgcttg cttcaattgg tccgcttttt ctgccttgat
    22261 tcggttcctg cttaactcag ctaagtattg ggccaaagtt tctgaggaaa tgttttctaa
    22321 gtcgatcggg gcactaaacg ccagcaataa ctctttagaa gcttttccga aaacgccctg
    22381 cttggtaaaa atcgtcgcgt attctggaaa gacttgatcc aatacggcta taatttttct
    22441 tttgaagtcg ctcgctgtac ctactaaata agttcggtaa cgagaaagtt gtttcaaaga
    22501 aaaaacattt tcatcggata agaccgtttc gacaaatgag ccatagcgca ttaagtcagc
    22561 aatcaaaaca gaatcaataa tatcgttttt tcgtttccgt atttctgtac ctttacgcca
    22621 tccgtctgtt tgaaggggat tcacaacgtg aatcaaataa tctgattcgt gaaggtatga
    22681 aaagatggac agccaatagt gccccgtggc ttccatccct ataacaaagt cactgggtgc
    22741 tccggaatag tcgaccattc gctcaattaa tgactctgct ccttcagtgg tgttaggaaa
    22801 tgaaaaacct tttagtaata cctttccttc ctcgttcatg atagaagcaa cgtgagtgcg
    22861 tttaccgata tcaatgccta aaaagtacat gtatatctga cctttcaaaa aaagaatttt
    22921 ggattgaaat ccactcaact gaaaagcgtc actaccttgt acgagatacg gagtaaagta
    22981 aggactttca acatctaact catacgcaat ccacttatca gagagaggag ccagtctctc
    23041 gagtgcgggt tcaatggccc aaggggggaa cggcttagcc tctcaatcca ataaatatat
    23101 tatctcaaaa gaaatgagtt aataccaaat taaaataacg atggttcata gggttatcca
    23161 atcaaaaccg gaaggttaaa a
//
LOCUS       DACEPD010000043        23067 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.044, whole genome shotgun sequence.
ACCESSION   DACEPD010000043 DACEPD010000000
VERSION     DACEPD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.044"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            493..1200
                     /locus_tag="IW797_001555"
     CDS             493..1200
                     /locus_tag="IW797_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1224338.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1259..1903)
                     /locus_tag="IW797_001556"
     CDS             complement(1259..1903)
                     /locus_tag="IW797_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ1224339.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            2030..2317
                     /locus_tag="IW797_001557"
     CDS             2030..2317
                     /locus_tag="IW797_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224340.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2425..2745
                     /locus_tag="IW797_001558"
     CDS             2425..2745
                     /locus_tag="IW797_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1224341.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2826..4139
                     /locus_tag="IW797_001559"
     CDS             2826..4139
                     /locus_tag="IW797_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ1224342.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4136..4855
                     /locus_tag="IW797_001560"
     CDS             4136..4855
                     /locus_tag="IW797_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ1224343.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4983..5534
                     /gene="raiA"
                     /locus_tag="IW797_001561"
     CDS             4983..5534
                     /gene="raiA"
                     /locus_tag="IW797_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ1224344.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5756..8290
                     /gene="secA"
                     /locus_tag="IW797_001562"
     CDS             5756..8290
                     /gene="secA"
                     /locus_tag="IW797_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ1224345.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8416..9517
                     /gene="prfB"
                     /locus_tag="IW797_001563"
     CDS             join(8416..8478,8480..9517)
                     /gene="prfB"
                     /locus_tag="IW797_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ1224346.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9622..10308
                     /gene="ftsE"
                     /locus_tag="IW797_001564"
     CDS             9622..10308
                     /gene="ftsE"
                     /locus_tag="IW797_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ1224347.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10292..11185
                     /locus_tag="IW797_001565"
     CDS             10292..11185
                     /locus_tag="IW797_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1224348.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11337..12785
                     /gene="cls"
                     /locus_tag="IW797_001566"
     CDS             11337..12785
                     /gene="cls"
                     /locus_tag="IW797_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1224349.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13122..13979
                     /locus_tag="IW797_001567"
     CDS             13122..13979
                     /locus_tag="IW797_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ1224350.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14094..15014
                     /gene="pstC"
                     /locus_tag="IW797_001568"
     CDS             14094..15014
                     /gene="pstC"
                     /locus_tag="IW797_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ1224351.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15014..15898
                     /gene="pstA"
                     /locus_tag="IW797_001569"
     CDS             15014..15898
                     /gene="pstA"
                     /locus_tag="IW797_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ1224352.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15913..16719
                     /gene="pstB"
                     /locus_tag="IW797_001570"
     CDS             15913..16719
                     /gene="pstB"
                     /locus_tag="IW797_001570"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224353.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16735..17493
                     /locus_tag="IW797_001571"
     CDS             16735..17493
                     /locus_tag="IW797_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224354.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17506..18183
                     /gene="phoU"
                     /locus_tag="IW797_001572"
     CDS             17506..18183
                     /gene="phoU"
                     /locus_tag="IW797_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ1224355.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18406..19437
                     /locus_tag="IW797_001573"
     CDS             18406..19437
                     /locus_tag="IW797_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1224356.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19434..20201
                     /locus_tag="IW797_001574"
     CDS             19434..20201
                     /locus_tag="IW797_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ1224357.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            20301..21869
                     /gene="liaX"
                     /locus_tag="IW797_001575"
     CDS             20301..21869
                     /gene="liaX"
                     /locus_tag="IW797_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ1224358.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22048..22353
                     /locus_tag="IW797_001576"
     CDS             22048..22353
                     /locus_tag="IW797_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ1224359.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF"
     gene            22353..22703
                     /locus_tag="IW797_001577"
     CDS             22353..22703
                     /locus_tag="IW797_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ1224360.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            22841..>23067
                     /locus_tag="IW797_001578"
     CDS             22841..>23067
                     /locus_tag="IW797_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1224361.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAH"
BASE COUNT         7597 a         3972 c         4964 g         6534 t
ORIGIN      
        1 gttagttcga gagctctttt ttttgctttg ctaccttgtt ccttgaagat tctgctgaat
       61 taagatagag ttaagttaac tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa
      121 gtagacgaag cagctgaaaa gttatatgat ttggtcaaat cagaaaaata caatattgaa
      181 gtgaatattc cgaaaaaagg cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat
      241 catacaaaaa aatggatggc taaaaaccgc taataataac aaaagatcca taagtcggca
      301 ttttcatcga ttttagggtc ttttgttatt ttctaggttg aacatatcta aaaaattgtt
      361 tctgacaata ggagtaggga gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac
      421 ctttatcttt ttcagttgtg aatcaggtat ataccagtta taatagaaga aacgaaagga
      481 cgggtgtacg ttatgtcgaa tcaattacct gtatatatac agattcatga ccaattaaaa
      541 aatgaaatcg aacagggtat atggaaagtc ggagaccgtc ttccttcaga aagagaatta
      601 gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa cttggcagat
      661 gaaggaatct tagaaagaaa gatcggttcg ggtacgtatg ttgcgcgaga aaaagtacaa
      721 gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg aatcacaaaa tcgagtaccg
      781 tctagtcgga caatctctta tttcttgact tcgccaagct caagtgaaat ggaaaaacta
      841 aacttgagta aagatgacac gatcttgcga atggaacgta ttcgtttcgc agatgatgtc
      901 cctatttgtt ttgaagttgc cagtgttcca caaaagctga tcgatggcta cagcaaagca
      961 gagattactg aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca
     1021 gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa ttgctgacta tttagaggta
     1081 aaacgaggag atgcagttct tcgtttgcgc cagatttctt attttgaaga cggtacgccg
     1141 tttgagtatg tgcgtacgca atacgtagga aatcgttttg agttttattt ggaaaaataa
     1201 aaaacaggtg atccctgatt ccttattaga aataaaagag aatcagaatc acctgttttt
     1261 atatatgatc agttatcagt tgctcgtgat aagaaacaga accctcttca aattggactt
     1321 gtccgttcag cagctcaatg atggattctt taaagtggtc agcacttttt tcatctacag
     1381 cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa tgttggagtt
     1441 caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag atctcctgtt
     1501 gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagaccgca tgagtgtatg
     1561 ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg actactgcag
     1621 ctacattgat cagttgattt tttttgagga cttccaacat cggtacgccc gccgttccac
     1681 ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg atgaaggcgc
     1741 tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa ctttttgctt
     1801 ggtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg atctcaatct
     1861 cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca cctctttttt
     1921 tcattttctt tattcagtat aaataaagtt ctaggttgtt tcaacactgt aacggttgcg
     1981 caattgctgt agtatgcgat aataaagaca aaggcaggtg gatctgggga tgcaggaacg
     2041 aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac aatggcggct ctcaagtaac
     2101 gttgaccttt acccaatttg acgaactgct gtttcccagt aacggacttc ccaaaacagc
     2161 aagggaaagt acagattggt gggcaaatga ttataaacac cctgaaaaag gtgcctatgg
     2221 ctggataaat gcaggctatg aagtagttgt catcaatttg gacaaagaat atgtcgtgtt
     2281 caataaatta gtgaaatcta gttggctatt tgattaaata aaccgcgttt agaaaacgtt
     2341 gacaaatttc tttcttttta tataatagag taacagacgc gaagaaaacg tttgcatagt
     2401 ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa tcatgcttgt atgttcggct
     2461 ggtatgagca caagtttact agtaacaaaa atgcaaaaag ctgctgaagc aaaaggaatc
     2521 gactcggata ttttcgctgt gtccgcttct gacgcagata acaaccttgc aaataaagac
     2581 gtggatgtgt tgttattagg cccacaagtt cgctttatga aagctgattt tgagaaacgt
     2641 ctggaaccaa aaggcattcc tttagatgtc atcaatatgg ctgattatgg tatgatgaat
     2701 ggcgaaaaag tgttagatca agcaatcacg ctcataggtg aataaataga acagtataac
     2761 tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt cgtatatttt tttagggcga
     2821 agaatatgaa tcatttttgg ggtagaaggc ttctgacgag agatattgaa gcaatagact
     2881 gtgaggaggt aaatttacca aattatgaga agatcgcagc agtggaacgt ttgggcaact
     2941 ggattctttg tcatcgctgt ggaatgagga cgtccgtcat tgatggacag cttgcagatt
     3001 acggttattt ttgtacacat tgtctatatt tagggcgatg cgacactcaa caagatttat
     3061 atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt ctttttttgg acaggacagc
     3121 ttactgaaaa acaaacggaa atctcaaaga gaatactttg ccattcagaa aaaagacatc
     3181 atcttatttg ggctgtaaca ggtgcaggga aaacagagat gctttatcct attttagtgg
     3241 aaactttaaa agctggagga agagtagcga tttgtacacc gcgagtcgat gtttgtaatg
     3301 aattgttttt acgctatcgt tatgtttttc cagaagaaaa aatcacgttg ctccatggga
     3361 atacagcaac ggcttatacc tttacttctt ttgttatctg tacgattcat cagctatacc
     3421 gtttttatcg gtcctttgat ttaatagtga tcgatgagat cgatgcgttt ccttataacg
     3481 gggatgcagg attagctcat gctgtgcaaa cagcgattaa accagacgga cgattcatct
     3541 acttaagtgc cacacctgat aaaaagttac taaaggaaat caagcagacg tttgacatcc
     3601 acaaacttgc tttacgcttt catcggcgtt tgcttcctga acccgtcttg gttttcatga
     3661 ataattggcg acaaaaatgc aagaacaaga agaagataaa atctttggtg cggatcattc
     3721 atcaattaat taaagaaaac catatccttt tattttgtcc aagtatcacg atgatgcatc
     3781 gtttgaaagc cacacttcaa gaggctcttc cgagatatga gatcacagat gtttcttcaa
     3841 gagacaatca gcgggcggaa aaagtccaga aaatgcgaga agcaaaatat gatatactgt
     3901 taaccacgat gatccttgaa cgtggagtca catttgaaag aatatccgtt atcgttttag
     3961 gagcggatca tccggttttt actaaatctt ctttggtaca aatcgcagga agagccgatc
     4021 gtaaagggcc ttatacgaac agtcaagtat actttttcta tgaagaaaaa actgcagcta
     4081 ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga agggaagaag atgctgtgag
     4141 gtgcagttgc tgcagtaaag atatcttcca aaatttaaca ttcagcgaga ttattggtgt
     4201 ttcgaaatac agattacctc agacttgttc agtttgtcgc gcacaatttc atttattgga
     4261 aacaagaggc tgttctggct gtcaaaaacc aatccatttc aatctagaga gtacgaaaaa
     4321 gaaagtaaag tctattcccg tttactgcag tgactgtcaa gaatggaaga agaaatatcc
     4381 gacctatgat tttcagcacc gtgccttttt tgtgtacgat gaaaatatga aggcctggct
     4441 caatcaatac aagtttatgg gagatatccg actagcaggc acctttgcat ataaatggac
     4501 gcagataaaa aaagaatacc cgacatacat ctattgtcca atacctttat ctgaaaaaag
     4561 gcgaacagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa gaattccttt
     4621 tcagatgcta ctcaaaagag agaagcatct gctggctcaa gcaaaaaaca cgcgaaagga
     4681 aagattggag atgcctcaac cttttgaact tgcagtcacc ttttctaaga tacaaaataa
     4741 aaaaatattg attattgatg atgtctatac aacaggacag actcttttcc acgcagcaga
     4801 ttgtttgcag gtcgcacagc cgaaaatcat ccgaaccatt tctttggcga gatgaattag
     4861 atgtggaaat ttgatagcga tttaatttgc aaataaccta gagttcgata taatagaatt
     4921 aagaagaggg aaagagtggt ccttctcctt cttgattgtg gtagacgaaa ggggtaattt
     4981 ttatgtttag atataatgta cgcggcgaaa atatcgaagt aactgaagcg attcgcgact
     5041 acgttgagaa aaaagtaggc aaactagaac gctatttcag tgacgcaccc gatgcaacgg
     5101 cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa agtggaagta acgatcccat
     5161 taccttatct tgtattacgt gccgaagaaa cttctccaga tttatacgca agtatcgatt
     5221 tggttgttga taaattggaa cgccaaatcc gcaaattcaa aacaaaaatt aatcgtaaat
     5281 cccgtgaaac gggcatgaat actgcagatg ctgctgcttt attcaatgaa gaggacgaaa
     5341 acgaaaatga ttctgaatta gatatcgtac gtacgaaacg cctttcattg aaaccaatgg
     5401 atagtgaaga agcagtctta caaatgaaca tgctgggaca taacttcttt atttttgaag
     5461 atgcagaaac aaatggtaca agtatcgttt accgccgtaa agatggaaaa tacggactga
     5521 ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa acttgcagca gctactgaca
     5581 gctgccgcaa gtttttttga gtttcctcct cttttagatg aaatatgaat aaattatgaa
     5641 gttcttactt taatacgctt aagatatttc aattatctat aactaatgat aaaatgaaaa
     5701 gtaaagtcta catagtctgg ataatacaca gaaggaaaag gagattacac aacgcatggc
     5761 caatttgatt cgttcactta tcgaaaacga taagaaagaa ctaaaacgtt tagatagcat
     5821 tgcaaaaaaa gttgaagcat acgctgacca aatggcagca ttaactgatg aacaattaca
     5881 ggcaaaaact gctgaattca agggcagata ccaaaaagga gaaacattag atcaattact
     5941 tccggaagct tttgcagtcg tccgagaagc agcaaaacgt gtattggggt tatatccata
     6001 ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga aatatccctg aaatgcgtac
     6061 tggtgaaggt aaaaccttga ccgcaacgat gccagtatat ctaaatgctc tgactggcga
     6121 aggggtacac gtagttaccg tcaatgaata tttagcaacc cgtgactcga cggaaatggg
     6181 cgaactttat aatttcttag gattaacagt ggggttgaat atcaactcaa aatcttcgga
     6241 tgaaaaacgc gaggcttata attgtgatat cacttatagt acgaataatg aattaggatt
     6301 tgactacttg agagacaata tggtcgttta ccgccatcaa atggtacaac gtccgctgaa
     6361 ttatgccatt gtcgatgaag ttgactcaat cttgattgat gaagcgagaa cgccgctgat
     6421 catttcaggg caagcagaaa aatccacagc tttatataca agagcagaca acttcgtgaa
     6481 acgtttgaaa gaagaagaag attataaaat cgatgtacaa tcaaaaacaa tcagtttaac
     6541 cgaagcaggg atcgaaaaag cagaagaaac attcggtttg gataacttat acgacattga
     6601 aaatactgca ttgacccatc acttggatca agcattgaga gccaactata tcatgctttt
     6661 agatatcgat tatgttgtcc aagacggaaa agtactgatt gttgaccagt tcactggtcg
     6721 gatcatggat ggacgccgtt attcagatgg acttcaccaa gcaatcgaag ctaaagaagg
     6781 cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc actttccaga actatttccg
     6841 tatgtacaag aaacttgcgg gaatgactgg tacagctaaa acagaagaag aagaattcag
     6901 agaaatttat aacatccaag tataccagat tccgacaaat cgtccggtta tccgtgatga
     6961 tcgtccagat ttactttacc caacattaca aagcaagttc cacgctgtcg tgcaagatat
     7021 caaagatcgc taccgcaaag gtcaacctgt gttagtcgga actgttgcgg ttgaaacatc
     7081 tgagttatta tcagatatgc tgaatcaaga acgtattcct catgaagtgc tgaatgcgaa
     7141 aaatcacttt aaagaagcag agatcatcat gaatgctggt caaaaaggtg ctgtgacgat
     7201 tgctaccaac atggctggtc ggggaacaga tatcaagtta ggacttgggg taagagaact
     7261 tggtggtcta gcagttatcg ggacagaacg acatgaatct cgtcgtatcg ataaccagct
     7321 tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca caattctact tgtcattgga
     7381 agatgatttg atgaaacgat tcggttcaga acgaatcaaa gctttcttag atcggatgaa
     7441 gatcggtgag gaagatgctg ttatccaaag caagatgctg acgaaacaag tagaatctgc
     7501 acaaaaacgt gtagaaggaa ataactatga cacacgtaaa aacgtcttac aatacgatga
     7561 tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga caagaagtaa tcatggaaga
     7621 aaaaagctta tccaagccac taatgaacat ggtcaaacgg acaatcagtc gtgtagtaga
     7681 tgcgcataca caattagaga aagaaaattg gaatttggaa agtatcatcg attttgccgg
     7741 cagtactctt gtccatgaag atagtattac tcttgcagat atcgaaggaa aaacatctca
     7801 ggagatcaaa gagtatctaa acgaacgagc acaagaagta tttaatacca aagcggctca
     7861 actaaatggt ccagagcaac tgttggaatt tgaaaaagtc gtgatcttgc gtgttgtgga
     7921 ctctaaatgg acagaccata ttgatgcaat ggatcaatta cgtcagtcaa tcggcttacg
     7981 tgcttatgga cagaacaatc cattagtcga atatcaaaca gaaggctata agatgtttga
     8041 agatatggta ggcgccatcg agtatgaagt cactcgaatc tttatgaaag ctgagatccg
     8101 tcaaaacgta cagcgtgaac aggtttctca aggaacagct tctcactcag aagacggaga
     8161 tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca atccatgtga acaaggtcgg
     8221 aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat aagaactgtc atggaaaaaa
     8281 tgcaaactaa tttctgacag caggattggt gagacaaatt ctttttttaa gaattcgtct
     8341 caccttcttt ctgttatttg ctgattctgt tagactagat ataatgaaaa aagtatagtg
     8401 aaagaaggaa aaaacatgga aatatccgaa attcgaaatc tcctgacaga aatgcaggaa
     8461 aaggtcacaa gtttcaggag gtctctttga cttagatcag ctggaagaag acatcgcgga
     8521 agcagaagca aaaatggctg aacctggttt ttgggatgac agcgaagccg cacaaaaagt
     8581 gatcagcgaa aataacgcta ataaagaaac ctatgaccaa ttccattctc ttgccgaaga
     8641 actagaagaa atcgaattga tggtggagat gctgcaagaa gaacctgatg cagcaatgca
     8701 agaagaagca gaaacacgta tcaatgcatt agatgaaaaa atgaagacgt atgaattatc
     8761 tatgctgtta gacggacctt atgatcgaaa caatgcgatc gtcgaattgc atccaggggc
     8821 tggcggaacg gagtcccaag actggggcag catgctttta cgtatgtata cacgttgggc
     8881 agagcagcat ggctatcaag tagaaacact agattatcaa gcaggtgatg aagcagggat
     8941 caagagtgtg accctgctga ttaaaggaca taacgctttt ggttatctaa aatcagaaaa
     9001 aggtgttcat cgccttgtac ggatttcgcc ttttgactca gcgaaacgac gtcatacttc
     9061 tttttgttcg gtagatgtga tgccagagct tgatgaaacg atcgatatag aaatcaatcc
     9121 agatgatttg aaaatcgata cgtaccgagc aagtggtgca ggtggtcagc acatcaataa
     9181 aacagaatct gctgtacgga tcactcactt gcctacagga acagtagtag ctagtcaggc
     9241 acaacgctca caattgaaaa accgtgagca agcgatgggg atgttaaaag ctaagcttta
     9301 tcaattagaa gtggaaaaga aagaacaaga agcagctgca cttcgaggag aacaaaaaga
     9361 aatcggctgg ggttcacaga tccgttctta tgttttccat ccctattcta tggtaaaaga
     9421 ccatcggaca aacgtagaga caggaaatgt ccaagcagtt atggacggtg accttgattt
     9481 atttattgac ggctatttaa aaatgcatct acaataagaa ctcattgtta aattgaaata
     9541 aaattgtaag attttgcaag catgttgaaa cattgccatg ctataatagc taaagactga
     9601 aaaagatatg gagagaacgc catgattgaa atgaaggatg taatgaagaa gtactcaaat
     9661 ggtacgaccg ctatccgcaa tctctcgatt gtcatcgaac aaggagagtt tgtctatgtg
     9721 gttggtccgt ctggagccgg taaatctaca ttcatcaaat tgatgtatcg agaagaaaaa
     9781 gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa tagcaatcaa aaaccgcgaa
     9841 gttccttatc tacgcagaga aatcggcgtg gtgttccaag actataaatt attaccaaga
     9901 aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta tcggcagaaa acctcgagat
     9961 atcaaaaaac gagtaatgga agtgctggac cttgttggtt tgaaacacaa agttcgagtc
    10021 tttcctagcg aattatccgg tggggaacag caacgtgtct ctattgctcg ggcaatcgtg
    10081 aatacgccta aagtacttat cgcggatgaa ccgacaggaa atctggatcc agaaaactca
    10141 tgggaaatca tgaaattatt agatcggatc aatgcccaag gaactactgt cgtgatggct
    10201 acacataaca gtacgatcgt aaatacgatt cggcaccgag taattgctat cgaaaacggc
    10261 cgcattattc gagatcaagc ggaaggggaa tatggatacg atgattagaa cctttttccg
    10321 ccatttatta gaaagtatca agagtttgaa acgtaacggc tggatgacag ttgcttcagt
    10381 cagtgcggta acgatcacgt tgactttagt cggtgtattc atggcagtta tcatgaatgc
    10441 aactaaatta gctcaggata tagaaggaaa cgttgatgta tctgtattcg tagatatcgg
    10501 tacaaaagac gaagagatga aaaagctaga aaaagaattg aatgacttag atcatgttaa
    10561 aaaagttgaa ttttcaagca aagaacagga attgaaaaaa atccaaaacg aaatgggtga
    10621 ttcctggaaa ttgtttgaag gtgacaataa cccgttatat gatgtgtatg tagtaagtgc
    10681 tactgatcct tcttatacaa aacaagtagc agaagaagca gcagatttga agaatgtttc
    10741 tcgttctgat tacggtggag cttcctcaga tcggattttc caaatcgctg gtgcagttcg
    10801 gacatgggga ttagttgcag cggcattgct gctgttcgta gcgatgtttc tcatttcgaa
    10861 cacgattcgg atcacgattt tgtctagaca gcgtgaaatt caaatcatgc gtttagtcgg
    10921 tgcaaaaaat ggatatattc gctggccgtt tttccttgaa ggtggctgga tcggtctgat
    10981 cggtgcgatt cttccgattt tgatcatcac atttggttat gcatggtttt tccaattgat
    11041 caatccatca ttattacgtt cccattattc attgattcgt ccacaaaata tcgtttggaa
    11101 aatcaatctt ttaatggtcg gcattggtgt gatcattggt tcacttggct cgatcatttc
    11161 tatgagacgt tttttgaaat tctaatacca caatataaat aaaatgagaa agttggacag
    11221 tttcgttcca gctttctttt tttattggct aggagaatag aaggaagcgg cttttttccc
    11281 tttactttaa aaaacaactt gggtattctt aagttggtag aagaagaggt gaagccgtga
    11341 aaattatcgt tgacaatttt tttacgatcc ttttgattat gaatattttg ctttccttta
    11401 tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg ggcatggctg ctcgttttga
    11461 tgttcattcc agttgtagga tttattttat atattttttt aggaagaggg atttccaaag
    11521 agaaaatctt tgatttgaag atccaaggga aaatcggtaa gaacctggaa attgaagaag
    11581 acaaacaggc attgatgcga gggctttatc ctcatccgcc tactggacaa gtagatgtga
    11641 aacaattgat ctatatgctc acggtatttg aaagtacgct ctatacgact aaaaatgaaa
    11701 tcactttatt tacagatggt cgtgaaaaat tcgatgcgct gattgaagat atcaagcagg
    11761 cagaagacca tatccacttc cagtattata tttatcgcag tgatgcatta ggtgaagaag
    11821 ttcgagatgc attgacagag gcggcaagac gaggggtaaa agtcagagtc cttcttgatg
    11881 catggggttc cacacaagtt tcttccagct tttttcagaa tttgaaaaaa gcaggcggag
    11941 aagtcgcatt tttctttcct ctatttgttc cgtatattaa tcctcggatc aattatcgca
    12001 atcatcgtaa aatcgtagtt attgatggga aaatcggata tacaggtggg ttcaatgttg
    12061 gaaatgaata tctaggattg gtaaagaaat ttggttattg gcgagacaat catttgcgta
    12121 tttacggaga agcggtgtat accttgcaaa accgtttttt gatggactgg aattctcagc
    12181 atacaaaaga agtaggctat tctcccaaat ttttcccgtc cattcacact actggtgata
    12241 tagctgttca aatcgtgaca agcggaccag ataccgaaca tgaacaaatc aaaatgactt
    12301 atttgaaaat gatttcgtta gccaaacggg agatattgat ccaaacgcct tattacatac
    12361 cggatggatc gattcatgaa gcactgaagt tggctcttct ttcaggcgta aaagttcata
    12421 tccaaatacc taacaaacca gatcatcttc tcgtttactg ggcaacgtat tctttcgctg
    12481 cagagctgat cgagtatgga gcaaggatcg aaacatatga gaatggattt attcatgcaa
    12541 agacgatgat tatcgatggt ggcattgttt cagttggctc agcaaatatc gatgttcgtt
    12601 catttcgctt ggactttgag gtcaatacac tggtttatga tgaacgaatg gcttcacgtg
    12661 taagaaaagc tttttttgaa gactcgaaaa tttctacgca tctcacaaaa gaaatgtatg
    12721 aaaaccgagg aataatcatc aagatgaaag aaggattagc aagattgatt tcgccgctcc
    12781 tttaaaacat aaaaaggaca tgagaaccat caacagatgg agatctcgtg tccttttcta
    12841 acggggccat ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta
    12901 actttggggt atctcttgtt ttttggggaa agcaagaaat aagctaatat atgctattaa
    12961 aagagacaca tttcaaagcc agtatagcat agaaactttt aatgagatag ttttttttac
    13021 aattatgaga atttattttg ttatttaatg attttgattc gttttcatca agtaatgaaa
    13081 atgataaaat aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat
    13141 tcttaccaat aataggatta gccattcttt tatcaggctg ttcaaagtgg attgatcgag
    13201 gagaatccat tacggctgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg
    13261 aaacttacca gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa
    13321 ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg
    13381 aagaaaaatc ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga
    13441 tcactcctat tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa
    13501 aactatttac aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag
    13561 ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg
    13621 acggagaaac agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg
    13681 ttgccgacac acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag
    13741 atacgttgaa tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga
    13801 cgatttgggc atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat
    13861 ttttagacta tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata
    13921 tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat
    13981 ctttacacaa aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg
    14041 ttcaatttgt acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag
    14101 atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat
    14161 tcatcagttt tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg
    14221 tagctagtaa aggattggcc acctttttta ctgataagat caatgtattt gattttctgt
    14281 ttggtacaga ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat
    14341 tgcctatgat cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat
    14401 ttgccattgg tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct
    14461 tacagccggt catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt
    14521 tatcagttat cgtgcctttt gtcagaagta tttttggcgg gacagggttt ggtatcttag
    14581 ctggtacatt tgtgttgttc gttatgatct tgccgactgt cacaacgatg actgttgatg
    14641 cattaaaggc agtaccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat
    14701 ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg
    14761 tttttggtat ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg
    14821 cagcattgat gccaacagga cttactactc ctgcttctac attgacgtcc atcttaacaa
    14881 tgggaatcgg aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc
    14941 tgatcttgct attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag
    15001 gagagctgaa ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat
    15061 agtttcgggg atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg
    15121 aataccgcac atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggtgg
    15181 tggaatcggg atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag
    15241 ttttcctatt tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct
    15301 aacagatgtg attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg
    15361 tttattcggt ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg
    15421 tgcattggct ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt
    15481 acaagcggtc cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga
    15541 aacagtgatg aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct
    15601 aagttctgga agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc
    15661 acctgcgtta gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt
    15721 ccgtcaagct gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc
    15781 tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg atcattgctg ttttactgtt
    15841 caacttcggc gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg
    15901 agaagaagca aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa
    15961 gagaaagaag tagcactata tacggatgat atacatgtct ggtatggcga caatgaagca
    16021 atcaaaggag tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc
    16081 ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat
    16141 acgaaaatca ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat
    16201 gtttatgaga tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag
    16261 tcgatttatg acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa
    16321 ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac
    16381 agcttggaca aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgcattgct
    16441 cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac
    16501 cctatttcga caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc
    16561 atcatcgtga cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc
    16621 tatctaggaa aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt
    16681 caggcaaccg aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca
    16741 aaagaaatta tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa
    16801 gggatcgatc taagtttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc
    16861 ggaaaatcca cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg
    16921 atcaccggaa gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt
    16981 gaactaagaa aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc
    17041 tatgaaaatg tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat
    17101 caagtagttg aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg
    17161 cataaaagtg ctttatcttt atccggcggg cagcagcagc gtgtatgtat cgcacgtgtt
    17221 ttggcagtta atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt
    17281 tctactggga aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc
    17341 gtgacccata atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat
    17401 ggtcacttga tcgaatacaa cgatacgaag aagatattca tgaatccaga aaaacaagag
    17461 acagaagatt atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa
    17521 tttgaagaag aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc
    17581 agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa
    17641 gttatcgaaa acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt
    17701 gaaatgatcg cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg
    17761 aaagctagct cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg
    17821 attcgcgtaa aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc
    17881 gattatgtga aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa
    17941 gacgcacgta cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat
    18001 aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac
    18061 ttgaatgttg ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg
    18121 atcgtctact tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc
    18181 taaactgaag ctgtgattat gccgtttgtt tatgaaaaga ggttgtgaaa aagctgttct
    18241 acatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg
    18301 acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa
    18361 aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt
    18421 agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagcc
    18481 gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga ggatcatcaa
    18541 ttgattacag gaaaactgat gaactcggat caaaaaggag aatcgattta tttatcggtt
    18601 ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc
    18661 caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg gcttttcttc
    18721 ttcacaagta tcttcattgg cgccatgttg cttattggcg gaaaacaggg tctatttgct
    18781 ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat ctttactaaa
    18841 acagaaggga tttctctatt aaataaatct atcctgttcg tcgttatagg gatgattatt
    18901 agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc agctgcagtt
    18961 acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg gaaagaccaa
    19021 ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt ttttctttct
    19081 agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt cattgcgaca
    19141 ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg gctttcagga
    19201 aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt cgtctatatc
    19261 agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcttt caaagaaacg
    19321 atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt aggaatagta
    19381 ttatcgatcc ccatctcggt cctctttttt ctgactgtaa ggaggctgaa acgatgaatg
    19441 tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc ggtacgcaag
    19501 gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg ttgttattcg
    19561 gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta ttgattgctg
    19621 tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct tttgtgagta
    19681 tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt ttagcgatcc
    19741 aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact ttagcgattg
    19801 attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct gttttagatg
    19861 cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg caattatctt
    19921 tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg tcaacagtaa
    19981 tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg tttattgatt
    20041 tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa tatacgatgg
    20101 ctattcttac cacattgtcc gcactaattg ttcttccttt aacctgcgtg actacagcat
    20161 atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa cttaagtcct
    20221 atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg caaacaattt
    20281 tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta gtgaaaaaag
    20341 gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact gaaaaagacg
    20401 aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa aaagataagg
    20461 caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag ccagttgatc
    20521 caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat gaactagcaa
    20581 ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc gctgacaata
    20641 aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa gaagaattag
    20701 gcgagttgac agaagaagaa ttagcacagc gtcaggcgtt agaggcagaa atcaaagagt
    20761 tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca gaattaaaag
    20821 atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag tttgaattgc
    20881 cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa aaaatgtcag
    20941 aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt tcagaaacag
    21001 tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt gcgacaacga
    21061 aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat atcaaggcag
    21121 caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt gaagctaaga
    21181 tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa cggagccaga
    21241 ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg cgtgcagacc
    21301 ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt ctaaatggca
    21361 acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg aatggtaaca
    21421 tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac ggcaatatcg
    21481 atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt acagtgacat
    21541 ttggtgcgac agcagaaaat ctttctgtgt ctttagtaaa tggtgatgta cgtcttacga
    21601 ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat gtcaaagtcg
    21661 ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga agtatcaata
    21721 gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac caaatgttgc
    21781 aattccgtcg tgtttcagat gatgagatcg cgcaagtgca actttcaacg acaacaggaa
    21841 gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt cagccttgag
    21901 caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg ccttattgcc
    21961 gataggctat ctcttgaaca ccgattaaga agaaggttgt cacaacgatt ttcttcattt
    22021 aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc caataaaaat
    22081 gttgttttga caggtacttt agcagggatt ggggaatata tcgggattga tcccactgtt
    22141 atccgtgtgc tttatgtatt cctaagctta gttgcttttg gaagtccaat cattttatac
    22201 attttattag ctttgattat cccatctgcg cgttctgcac gaacaaacta tggacataac
    22261 aatgcatatt atgaaaaaaa caattatcgt gaacaaacaa aaaaacgcaa agaagcggaa
    22321 aaaatcgatg atgacgaatg gagtgacttt taatgtcgta ctttcagcgt attttggtta
    22381 atacgttaac attcatatcg ttggctgttc tttttcctgc gatggtccat gtgagcagta
    22441 tttggacagc cgtccttgcc agcttcgtat tatctgtgtt gaacatgttt gtcaaaccga
    22501 ttctgacgat cctgtccctg ccctttacat tagtcacatt gggattgttt acgtttgtta
    22561 tcaatgcagc gattcttaaa atgacttcct tttttgtagg agcagaacat tttagttttt
    22621 cttcatttgg tgcagcgtta ttcgtagcta tcttgatgtc acttgtcaat gtaatcgtta
    22681 gtgaacataa cagcagaaag taaaaaaagc tgtgccgaga tctcggcgca gcttttttgt
    22741 ttatcgcgaa ttgtaaaatt aagttagaaa aataaaaagg catttatggt acactcaaat
    22801 tgtatttccg acgaaagaaa acaaaggagt gtaaccataa atgacctaca aacatcttac
    22861 catagacgaa ctgacaatga tagaatcata ttatcttcaa cataataagc cggttgaaat
    22921 cgctaaccga atgggacgtg ctatacaaac tatttataat gtagtcaata agttcaagca
    22981 aggtaagact gctcttgatt attggcacca gtataaagaa aataagaaaa aatgtgggag
    23041 aaaagtcatt caattacctg ctcatga
//
LOCUS       DACEPD010000044        22922 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.045, whole genome shotgun sequence.
ACCESSION   DACEPD010000044 DACEPD010000000
VERSION     DACEPD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.045"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(161..445)
                     /locus_tag="IW797_001579"
     CDS             complement(161..445)
                     /locus_tag="IW797_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224362.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(463..1353)
                     /locus_tag="IW797_001580"
     CDS             complement(463..1353)
                     /locus_tag="IW797_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ1224363.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1373..1591)
                     /locus_tag="IW797_001581"
     CDS             complement(1373..1591)
                     /locus_tag="IW797_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224364.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1561..3261)
                     /locus_tag="IW797_001582"
     CDS             complement(1561..3261)
                     /locus_tag="IW797_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ1224365.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3528..4301
                     /locus_tag="IW797_001583"
     CDS             3528..4301
                     /locus_tag="IW797_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224366.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="IW797_001584"
     CDS             complement(4382..4951)
                     /gene="thiT"
                     /locus_tag="IW797_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ1224367.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5017..5119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5125..6096)
                     /locus_tag="IW797_001585"
     CDS             complement(5125..6096)
                     /locus_tag="IW797_001585"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1224368.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="IW797_001586"
     CDS             complement(6190..7563)
                     /gene="glmU"
                     /locus_tag="IW797_001586"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ1224369.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8066..8884)
                     /gene="purR"
                     /locus_tag="IW797_001587"
     CDS             complement(8066..8884)
                     /gene="purR"
                     /locus_tag="IW797_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ1224370.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9051..9875)
                     /locus_tag="IW797_001588"
     CDS             complement(9051..9875)
                     /locus_tag="IW797_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1224371.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9848..10528)
                     /locus_tag="IW797_001589"
     CDS             complement(9848..10528)
                     /locus_tag="IW797_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224372.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10551..11489)
                     /locus_tag="IW797_001590"
     CDS             complement(10551..11489)
                     /locus_tag="IW797_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ1224373.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11734..12585)
                     /locus_tag="IW797_001591"
     CDS             complement(11734..12585)
                     /locus_tag="IW797_001591"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ1224374.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="IW797_001592"
     CDS             complement(12739..15312)
                     /gene="mprF"
                     /locus_tag="IW797_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1224375.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15453..16259
                     /gene="proC"
                     /locus_tag="IW797_001593"
     CDS             15453..16259
                     /gene="proC"
                     /locus_tag="IW797_001593"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ1224376.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16338..17894)
                     /gene="rny"
                     /locus_tag="IW797_001594"
     CDS             complement(16338..17894)
                     /gene="rny"
                     /locus_tag="IW797_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ1224377.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18305..19354)
                     /gene="recA"
                     /locus_tag="IW797_001595"
     CDS             complement(18305..19354)
                     /gene="recA"
                     /locus_tag="IW797_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ1224378.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19463..20704)
                     /locus_tag="IW797_001596"
     CDS             complement(19463..20704)
                     /locus_tag="IW797_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ1224379.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="IW797_001597"
     CDS             complement(20944..21522)
                     /gene="pgsA"
                     /locus_tag="IW797_001597"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ1224380.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21731..22615)
                     /locus_tag="IW797_001598"
     CDS             complement(21731..22615)
                     /locus_tag="IW797_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1224381.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
BASE COUNT         6521 a         4990 c         3969 g         7442 t
ORIGIN      
        1 taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag attgtgacac
       61 gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg aagaaatcca
      121 attctgatta tttattgtat ttctttgttc agcatccttg ctaaaagccc atattggaca
      181 aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct gtcggatctt
      241 ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg taagcaccgt
      301 cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct ttccatacgt
      361 caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca cttaattcgt
      421 ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa tattttgtac
      481 ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt taatttcgat
      541 tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga tcaggaaatc
      601 taattcccga cctacaggac attgtgcttc gataagttca tctagttttt gtacatggat
      661 cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg ctagctccgt
      721 caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt tttgatacct
      781 tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta gtacttcttt
      841 gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt tttctcgact
      901 cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg ccaattcttc
      961 catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagcg cgaagcgttc
     1021 cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa ttccgtctgt
     1081 caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt tttcgttttt
     1141 gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact gctgtttaat
     1201 aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta tttgaacatc
     1261 tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt ttgtttcgcg
     1321 gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct cattacatat
     1381 tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg cctttcccca
     1441 ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga ccattccagc
     1501 tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata gcgatttctt
     1561 ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat aatataaata
     1621 taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac gatccttgcg
     1681 ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt gtattttttg
     1741 ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt gtgccaataa
     1801 atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca tgtctacttg
     1861 atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca tcgccaatcc
     1921 gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc aaaattttat
     1981 gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca aaaatcctcg
     2041 ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat agtaatagcc
     2101 taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc tttggctatc
     2161 aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga taaaaatgtc
     2221 tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa atgcctctgt
     2281 tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac cgtctgcata
     2341 tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag aggcgagtat
     2401 ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt ccgctagttc
     2461 tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga gtaaggacaa
     2521 atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc cttcaattag
     2581 tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt cttcaactgc
     2641 agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata aattcacagc
     2701 ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg aggtacctgt
     2761 gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa tctgataagc
     2821 accgtgcgct cctcctccac ctaaaacaag tgctgtatga tagaccagct ttttatttaa
     2881 ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt ctgatagagg
     2941 agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag cttccatata
     3001 ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat taaaaataac
     3061 atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata ctgctttaaa
     3121 agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat gactttgaaa
     3181 aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt tctgtgaaaa
     3241 gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca gtacgtcata
     3301 aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa ttcataaaac
     3361 ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag agtgttttca
     3421 atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt tttaatgcaa
     3481 atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg actgagtcag
     3541 tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg actgctactg
     3601 actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat ccatctgctt
     3661 ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca gaaaatattc
     3721 agcgtttctt ttcctcttct cgtaacaggc gtggattcga tgaattgaag tggatcgact
     3781 ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa ttattatatt
     3841 ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag aataattttg
     3901 tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta gatgaatttt
     3961 ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat gtaaaaaaat
     4021 cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg aaagaaatgc
     4081 gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg atcgatggta
     4141 aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa ggcacccggt
     4201 atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga cactggcaat
     4261 tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc ttatcaaaaa
     4321 ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc ttctttttta
     4381 tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt tggatcccta
     4441 ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc attacgaaag
     4501 aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc actcctgcaa
     4561 taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg atgctttttc
     4621 taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc atgagccctg
     4681 ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg acacttaaat
     4741 aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc agcatccctg
     4801 cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat gaaatagaaa
     4861 aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct gcaacaatcg
     4921 ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg aataaaaaag
     4981 tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca caattcctac
     5041 gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc agcccaattg
     5101 gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg gactaactgg
     5161 tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac ttacttcatc
     5221 gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa ctagacgctc
     5281 gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat gtgtacatga
     5341 agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg tgatcgtacc
     5401 tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt gtccaatgat
     5461 attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg caatcggaga
     5521 tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg gtgaaacaac
     5581 gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa gtggcgcacc
     5641 catcaaatga tcgactggaa tatcaaagaa accttggatt tgaactgcat gaaggtccaa
     5701 agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt tcgctgtgat
     5761 tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag gcatgactac
     5821 gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca actccatcaa
     5881 gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc cacgaatact
     5941 ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg attttcctaa
     6001 ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat ttaacgcaaa
     6061 aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt tctttttcac
     6121 aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc aagtgatttt
     6181 gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat tgacttgtct
     6241 tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg acccggcagc
     6301 aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag aaccaataaa
     6361 gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga cgaagacaac
     6421 accacatccg acattgattt cttctcccag cgtagcatca ccaacataag tcaagtgacc
     6481 gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc cgatatggac
     6541 gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat ctgcatgttt
     6601 cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct caatcctact
     6661 gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg tatttccttg
     6721 gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg catcgatgta
     6781 tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt taattcgctt
     6841 tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca ttgattcttc
     6901 aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga tttcgatgat
     6961 atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta acgcatcgaa
     7021 caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa gagcttcttc
     7081 tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt cacggatgat
     7141 tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac ttgcattttt
     7201 tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg gtgtatcgcc
     7261 actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt gcaaaacagc
     7321 atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc ctagttgtgc
     7381 tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt tcgtttcact
     7441 gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta aaactttata
     7501 taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat aacgtgcgtc
     7561 caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta tacccatttt
     7621 ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa aatagataga
     7681 ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc ggtcacaagt
     7741 cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg acttagagtt
     7801 aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc ttcggtatta
     7861 gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta atactcagga
     7921 caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt tctcggtatt
     7981 aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct tactactcga
     8041 aactaagcag ctttttcaca acctcttatt ctgaaaaata atttcctggc acaaccgtaa
     8101 tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa tcatcgatgg
     8161 cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt tctgattcga
     8221 attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg aaatcatcca
     8281 ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc ttttcaattc
     8341 tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt atttttgagt
     8401 cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg gcaataggta
     8461 cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat ttagaagcaa
     8521 tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag agataaacat
     8581 atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat tcaatatatt
     8641 ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca gcacccggga
     8701 ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt cccaaatctt
     8761 cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag gaaatcaatt
     8821 catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg cgacgtactt
     8881 tcacctattc caactccttt taagaaattt tatcgtttta cattatataa gtttttcttc
     8941 aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt aaaataaaaa
     9001 gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat ttagttattt
     9061 tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa caaagcaata
     9121 aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct agttagtcct
     9181 gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt gaagcttttg
     9241 cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa aagtgcaccg
     9301 gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat catcgacatc
     9361 aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag aacatacatc
     9421 ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc taaaaagatg
     9481 acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat acttgttgct
     9541 gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc agccattaaa
     9601 atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc caatatcaca
     9661 gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa aaagaatcct
     9721 aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga ttgtcttctg
     9781 atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc ggcaagaaac
     9841 gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt ctcaccagtc
     9901 ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc attagaactg
     9961 atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt ctggattctt
    10021 catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta gcaaaaatac
    10081 gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc cgatttcgca
    10141 tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct ttttcagaga
    10201 gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg atcaccgtac
    10261 taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata cttaattttt
    10321 ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc agtaatccta
    10381 acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa atgacaaatt
    10441 cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca tagtaaaagg
    10501 tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac ttaattgata
    10561 ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc gcctgctttg
    10621 atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac tcctgtttca
    10681 ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat cactttgatg
    10741 ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg ttcctgatct
    10801 ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa
    10861 gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc ggagtatttt
    10921 tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa agtttccttt
    10981 ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt tatagctaat
    11041 accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc ttcttctgat
    11101 tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc tgcttcgatc
    11161 atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct
    11221 gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt tgcacttggt
    11281 tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct
    11341 acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt caaatcctct
    11401 tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc acctatcatt
    11461 accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa caaatcggaa
    11521 ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt caatagaaat
    11581 ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg gatgagttgc
    11641 tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt taggattcct
    11701 cagtttttca tcacctattt tacttcttat attttattgc agggttcgta ctaaataaac
    11761 ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat aatgttgaca
    11821 caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc cgtacttgat
    11881 catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat cttctaaact
    11941 gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt cagcaagtcc
    12001 gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg tacgtgtaga
    12061 gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag gtaatacttg
    12121 gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt aaggaacatc
    12181 tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca gattccataa
    12241 ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta atccagctgc
    12301 tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt atttattttt
    12361 tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa atgctttatt
    12421 tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat ctgccaagtc
    12481 tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat ataatacatc
    12541 cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct cacccattcg
    12601 aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc ttatgtctgc
    12661 ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct tcataatcca
    12721 aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac tcggaacatt
    12781 tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga aattacttcc
    12841 tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt tcttttcata
    12901 agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca tgactttttc
    12961 ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc ctagattggc
    13021 ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat agtcagctaa
    13081 ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct tcttcgtcat
    13141 cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac cgacaccgct
    13201 agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc gtgcaccttg
    13261 aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca gttgatcgta
    13321 ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct gactcagccg
    13381 agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg tgaagtgaaa
    13441 acccaattca tgaagcatca ctacgtagct ttctgatatc ttataaaacg caagctgata
    13501 gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca ttttaggatt
    13561 gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac caaaaacgac
    13621 ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc gaagctgatg
    13681 gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt tttcgtactc
    13741 actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta aagctagcaa
    13801 tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa acaaaataaa
    13861 atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg caattgcgta
    13921 aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc aagaataaac
    13981 gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa tcaagacata
    14041 taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac caaaagatag
    14101 taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc tagcaaggcc
    14161 gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga atgagaacgg
    14221 caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg atagaagtac
    14281 catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa ccttttggaa
    14341 aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat gtgtgatgaa
    14401 caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat ataatagcca
    14461 aactactgct acatcttgcc gaacaggcaa ttgactcatt cctaagatca tcaaaacgtc
    14521 aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag caatcatgaa
    14581 cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga tgatcaaaaa
    14641 taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa gcaagccttt
    14701 tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga aaatgactgg
    14761 tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac tttctgactg
    14821 aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag tcagcataaa
    14881 tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct ttccataaaa
    14941 actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt
    15001 tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc cttgtttttc
    15061 taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca gcagtccagc
    15121 cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc aaatatcttg
    15181 ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta acacggagcc
    15241 gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca tcaagtatcg
    15301 tttatttttc acattttccc attcctaact tgtatacttt tgtgtcatac cccaatgtta
    15361 tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg atagaaaaag
    15421 aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc ggcacaggga
    15481 atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg aaagaagagg
    15541 tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc
    15601 atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt ttagccgtga
    15661 aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag aaagtcttat
    15721 tcatttctat tgcttcagga aaatcattaa cagatttaga aacagcgctt gaagatcctc
    15781 aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg
    15841 cgatttgcaa aaatgaagct gccacggagg aacagcttca gcaagctacg atgatttttg
    15901 aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc attggccttg
    15961 ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg actggtgtac
    16021 tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt atgggttctt
    16081 gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa gtttcttctc
    16141 ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc atcccagcaa
    16201 tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat aaaaaataat
    16261 gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt gccgcggctc
    16321 ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg aataacgatt
    16381 actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat atcacgtact
    16441 aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg aacttcgcgt
    16501 ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat attttccaga
    16561 cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg tcttgctgct
    16621 gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc cacatctcca
    16681 tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc agctaattcc
    16741 gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc aatatcatgg
    16801 agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc taagatacca
    16861 gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact agttctgaat
    16921 cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc gacttcaaac
    16981 gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga tttctcgacc
    17041 atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc caaggtcatc
    17101 cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc cggtgtatca
    17161 tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg accttctcgt
    17221 ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac agtcgtttct
    17281 gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt cgctttgcga
    17341 tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa ctcatgattc
    17401 agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga taaaccagca
    17461 attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt ttctcgctct
    17521 tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga gttctcgcgt
    17581 ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa acgattctct
    17641 tgagatttta attctaactt actttctctc agctcacttt caacttcaga acggtatttc
    17701 tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc ttcttttttt
    17761 gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt
    17821 gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat agcgaggaga
    17881 atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc atctatacag
    17941 cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat gtgtacaaaa
    18001 aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa ataaacaaag
    18061 aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca ctagtttcat
    18121 ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta gatgaacaga
    18181 tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct ttattcaata
    18241 aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt gtttatcata
    18301 atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct gggacagcga
    18361 cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc atcatttcag
    18421 gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga tcttctttat
    18481 aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg tctagaagtt
    18541 ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt ttgaaaggag
    18601 gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata tctgtacctt
    18661 gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc
    18721 gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca cggatttggt
    18781 tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta cgcaatgctt
    18841 gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg tcgatttctg
    18901 ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc ccacttgata
    18961 ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt
    19021 catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc tcagcatcaa
    19081 tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt gcaactgttg
    19141 ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg taaccgccga
    19201 cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt tgttggtcga
    19261 ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt tttttcagtg
    19321 cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt atatttacaa
    19381 atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa
    19441 accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa agatcaaatc
    19501 cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga tatttttccg
    19561 aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat tgaccttcta
    19621 acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt gctatctcac
    19681 gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat tctgctaata
    19741 gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta acgaatccgc
    19801 ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg
    19861 tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc actacgactt
    19921 ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt tcttggattt
    19981 tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt ttcactgtca
    20041 gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg atctgtgtct
    20101 caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa
    20161 ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt
    20221 ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac gcatgtgtat
    20281 ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga accccgcctt
    20341 cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt gtttcaaaat
    20401 atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg aggtgagccg
    20461 cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat aagagtatga
    20521 gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga ttatctccta
    20581 caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac aagaaagtgg
    20641 cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt atttctgctt
    20701 tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt ttatttaaaa
    20761 agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt
    20821 tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa agctgttttt
    20881 tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc cactaaacga
    20941 tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca cacctgaata
    21001 gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac cgatcaggct
    21061 gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag ccgtcttgac
    21121 ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta aacgcaagcc
    21181 tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg cctttccttg
    21241 gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg ggtccgcaaa
    21301 cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta accagtctgt
    21361 gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca gtgtggtgtc
    21421 tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga aaatcgggat
    21481 catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga ctcctttctt
    21541 ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt tccaaatgaa
    21601 tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa gaaggaccgc
    21661 gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat gcgtaaacta
    21721 atttatacga tcattctgcg tattgaagag taaggttcac attttttgga ctctgattgt
    21781 tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca ttgcttgctg
    21841 caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc tgtgtttcat
    21901 ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct aaccatacac
    21961 gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata tttactgcga
    22021 tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca cttgattttg
    22081 ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag gaagaggact
    22141 cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt ggatcagctt
    22201 gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca accaatccta
    22261 ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc tgatgcattg
    22321 cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat cctgtcaatt
    22381 tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca gcttcagcgt
    22441 attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt
    22501 cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca tctaaagata
    22561 tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt
    22621 catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa agacgttatg
    22681 tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa cattgttctc
    22741 ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct gttttttgaa
    22801 cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt aaacggtgtt
    22861 caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag atatttttcc
    22921 gt
//
LOCUS       DACEPD010000045        22890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.046, whole genome shotgun sequence.
ACCESSION   DACEPD010000045 DACEPD010000000
VERSION     DACEPD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.046"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(290..1087)
                     /locus_tag="IW797_001599"
     CDS             complement(290..1087)
                     /locus_tag="IW797_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ1224382.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1084..2268)
                     /locus_tag="IW797_001600"
     CDS             complement(1084..2268)
                     /locus_tag="IW797_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1224383.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2293..3540)
                     /locus_tag="IW797_001601"
     CDS             complement(2293..3540)
                     /locus_tag="IW797_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ1224384.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3755..5500
                     /gene="dnaX"
                     /locus_tag="IW797_001602"
     CDS             3755..5500
                     /gene="dnaX"
                     /locus_tag="IW797_001602"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ1224385.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5520..5834
                     /locus_tag="IW797_001603"
     CDS             5520..5834
                     /locus_tag="IW797_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ1224386.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5859..6455
                     /gene="recR"
                     /locus_tag="IW797_001604"
     CDS             5859..6455
                     /gene="recR"
                     /locus_tag="IW797_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ1224387.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6519..7163
                     /locus_tag="IW797_001605"
     CDS             6519..7163
                     /locus_tag="IW797_001605"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ1224388.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7178..7507
                     /locus_tag="IW797_001606"
     CDS             7178..7507
                     /locus_tag="IW797_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224389.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7521..8459
                     /gene="holB"
                     /locus_tag="IW797_001607"
     CDS             7521..8459
                     /gene="holB"
                     /locus_tag="IW797_001607"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ1224390.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8495..9319
                     /locus_tag="IW797_001608"
     CDS             8495..9319
                     /locus_tag="IW797_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ1224391.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9312..9659
                     /locus_tag="IW797_001609"
     CDS             9312..9659
                     /locus_tag="IW797_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ1224392.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9728..10600
                     /gene="rsmI"
                     /locus_tag="IW797_001610"
     CDS             9728..10600
                     /gene="rsmI"
                     /locus_tag="IW797_001610"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ1224393.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10709..11830
                     /gene="dinB"
                     /locus_tag="IW797_001611"
     CDS             10709..11830
                     /gene="dinB"
                     /locus_tag="IW797_001611"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ1224394.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="IW797_001612"
     CDS             complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="IW797_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ1224395.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="IW797_001613"
     CDS             complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="IW797_001613"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ1224396.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15316..16083
                     /locus_tag="IW797_001614"
     CDS             15316..16083
                     /locus_tag="IW797_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224397.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16073..18118
                     /locus_tag="IW797_001615"
     CDS             16073..18118
                     /locus_tag="IW797_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1224398.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFF
                     PPMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18399..18548
                     /locus_tag="IW797_001616"
     CDS             18399..18548
                     /locus_tag="IW797_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ1224399.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18559..20073
                     /gene="dltA"
                     /locus_tag="IW797_001617"
     CDS             18559..20073
                     /gene="dltA"
                     /locus_tag="IW797_001617"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ1224400.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            20070..21275
                     /gene="dltB"
                     /locus_tag="IW797_001618"
     CDS             20070..21275
                     /gene="dltB"
                     /locus_tag="IW797_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ1224401.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21320..21553
                     /gene="dltC"
                     /locus_tag="IW797_001619"
     CDS             21320..21553
                     /gene="dltC"
                     /locus_tag="IW797_001619"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ1224402.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21556..22824
                     /gene="dltD"
                     /locus_tag="IW797_001620"
     CDS             21556..22824
                     /gene="dltD"
                     /locus_tag="IW797_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ1224403.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
BASE COUNT         7172 a         4114 c         4730 g         6874 t
ORIGIN      
        1 ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac
       61 ttgtgaccga ggagttggta ggagaacacc gtttaccaag aggttgtgaa aaagctgttc
      121 tgcatcaagt aataagaaga gccaagtaaa aacagtctct tatgttttta acttggctgg
      181 acttatcacc gtagatgtaa gcttttgaac gccgtttatt tgcaacaaaa ggggctgtga
      241 cacgattttt gtcacagccc tttttttgcg aacattcatt tagcaatttt tagaaacttc
      301 tgccgccacc gccaaaagtc ccgccaccgc tagaatgggt agtacttccg ccaccgccgc
      361 ttccaggagg tgggcttttg gggattcttc ttgtcgtgac aaaagaattt gtcagacgat
      421 cggttttttc agttaatttg atactggatt tttctcggaa tggatatttg tagatgcctg
      481 atttcaattg ataacgcgaa acagtcacaa aataaaagac aagactaagg ataagagcca
      541 aaacaacagc aatgatgatt tctactgtcg tcaaaacctt atagcgtgta attttgccag
      601 tttcttcgtc tacacgataa tgtcctcctg gaactccgtc ttccacatat gttgctgatt
      661 tagacaggta agcttttgca gcattgtaat aggcagcatt tgtcatctga tcataaacat
      721 catctaagat cgaatcgatt ctgctatcag tcaaataatc gatcatatta ccagaagtcg
      781 aaatgtagat ttcacgctgt cccatatcta atagcaatac tgagccattt tgatcatttc
      841 cgactgcgtc tcttaagtaa ttatcagcaa agtctcttgg ttcttctgaa ttagatgtag
      901 tcgttacgat caagactcgt cctttgattt tttcattcag cttttctgcc tgttgttcca
      961 aagattggat ttgatctgtt gtgaataacc cagcctcatc atttaccgta gctgtatctg
     1021 catgagtagc caccggccag ccaacactta ctagcaaaag aaagaataaa aaaaattttt
     1081 tcatcatatc aagtaacctc cgatcagaaa gaggatcgct agcccagcga aaatcccaag
     1141 agcggtcagt cccaattttt tctggctgat cggcaggaca ccgctgacct ttcctgtttg
     1201 tccattcatt gcgtagtagt agactttttt acttgagtca ttgcttcgat aagtaacaag
     1261 ccataccggc agtaatacgt aattatttac ttcgtttgtg attgaagcag aagttcgcac
     1321 accggtcaat gttgtatatc catttgcggt atcttttaac agcttctctg aatagtcttt
     1381 taattcagat tgaatctgtg atttgattgc ctcatattcg atatctcttt tttctgcttg
     1441 gaacccggct aaatactggc ttttaaaatc aattgcttta tctaacggga aaggttgcac
     1501 gccttcgacc atcttttgtt gcgtattttt tgataaggca ttttttacta gttctttaaa
     1561 agaaagcttg cctgctcttg aaacagcaaa ttgcttcgtt tctgtgtatt cgatgtcacc
     1621 tatccgccat atgcggataa ctgtaccatt cgcctgcatg gcaccatcta cttcagcatc
     1681 tgtagcccaa taagggaaat acacacctgt caacttatcg atttgatcct tattgaaaaa
     1741 agctttggga acaaaccatt ttttcttggt ccaagcgaga aatctgttga ttgcttcttc
     1801 tttctcgaca gcaaaaggaa gtactttatt tggcaagaat tctccgctta aacgtcccga
     1861 tagtacgacg ggattatggc agtagtaaca ataagtcgca gcagttgtcg catcagtcac
     1921 gatctctgca ccacagttgg gacaaagaaa aagatctatc gctgcggtat tatctgcaga
     1981 ttcgtccgct gtatctgaaa aaccgcctgc ctcgtcaacc gctttgcgct cggcttcatt
     2041 catatcggca agctgttctt gggctgtaaa agtaaaatct tcggtaggtg aaggttcttc
     2101 tttgctttct tcgccagcca tacgcgcttc tttttgcttt tgctcatatt ggctgacttc
     2161 ttcttctgta tagatattca ggcagtattc gcaatgaaat ttttggtctt tgggatcaaa
     2221 agtcaacgga ccgccgcaat taggacattt atgtgtaaga acatccatca gctgcacacc
     2281 tttctaaata aattaattaa cggggattcc tttaaacggt ttaccacagt ttggacagaa
     2341 tttagggatg ccgttggaaa gatccacgat ctcattgcat tcactgcatc gcatttgtag
     2401 cttaggctga acgttttcta caggtttggc agctccgcag ttggaacaga attttccgga
     2461 attttctgtc ccgcaaactg gacatgtcca agtatccgca tttccttgtg cagtttgttg
     2521 attttgtttt tctgcttgtt gtttgatttg ttcttggtta ttttgagctg cttgagaaag
     2581 ataagaacca tttgcattca ttcccatacc tacacccata aagccggtca ttgctcctgc
     2641 ttcatttttt ccggctgctt ccataccacg tgcaatggaa ccttgcacgt atccttcacg
     2701 tacgctagga tcacctagca tcgccccttc gttacgcata ttgatcagtt tcacagaatc
     2761 atcggtgtaa gaaatgctgg ctacagccac agaaacaatc tccatcccac gtaattcacg
     2821 ccaagaatca tccaatgcag tatccatgta tttgcttaat tccaaactct tagatggtac
     2881 ataagaaatc cgttgaccat ctgcagacat ctgattgatt gctgattgta gcgctgttag
     2941 gaattcagct aaatactgct cattgatatc attgatttcc acttgtgttt tgtttttagg
     3001 gattgcgttt gtataaaata aaatcgggtc aacgacatga atagaataag tgccatgtgc
     3061 ccgtaagaat aattctgcgt tatagaaatt gtcaaagtag ttcaacggcg cgcttgtacc
     3121 gaatttgatt ccctttattt cttggagatt gatataaaaa acttgttgtt tctgcggtgt
     3181 gacgccgcca aatttgaatc ggtcaaaagt ttcagcaatt gcttctttca gtgttccatt
     3241 gaacatggaa ggagctgcgt catttttaat ggtgtaataa ccttcctcag cggtataatc
     3301 gatgattttt ccaccatcga ccaaaagcat catcatgttt ggatagacat ggacgactgt
     3361 tccgtctgtt aaaacatctt ctgttccttt acgattggag ccacgtttgt catctttgcg
     3421 gactttgaca ccttttgtca tgaccgttgt atcactcata ttgtctggtt cgatgacttc
     3481 tagccattga tctgccagac cgccgccaac catacttgtt gctgctttga taagacccat
     3541 aaaaaattcc tcctctgatt cagctagtgc caaacaatta ttgtttattt attataccat
     3601 aggaagatgt gaaacttctc ggactagaga cggaattgaa atggaaaatt gcgtattgtt
     3661 taaatacctg aaaaagccga gaaggatagt agttttttta cctaatatca cgtataataa
     3721 gagatagtga attttttaaa aaagggggat ccttatggca tatcaagcac tttatcgagt
     3781 ctggcgttcg cagcgtttcg aagacatcgt tggacaaaaa gctgtgacac agacactaaa
     3841 aaatgctatt gtttcccaca aaacctcaca tgcctacttg tttacaggac ccagaggaac
     3901 cggaaagacg agtgcagcaa agattttcgc aaaagcaatc aactgtccga acagtaaaga
     3961 cggtgagcca tgcaacgaat gcgaaatgtg tcgatcaatt actgctggta cgcaagaaga
     4021 tgtgatcgaa atcgatgctg ccagcaataa cggagtcgaa gaaatccgat ttatccgtga
     4081 tcgggcaaat tatgcgccta caaaggcagc gtacaaagtg tacattattg atgaggtaca
     4141 tatgctttca acaggagctt tcaatgcctt gttgaaaaca ttggaagagc caaaagaaag
     4201 tgtcatattc atcttagcaa cgaccgaacc tcacaaaata ccagctacga tcatttcacg
     4261 gactcagcga tttgatttta aacggatcaa tacggcagat attgtcgaac atcttgaatt
     4321 tatcttgaaa gaatcgacta tcagctatga accagctgca ttgaatgtga ttgcccgttc
     4381 tgcagaaggc gggatgcggg atgcattgag tattcttgat caggcaatct cgtttagtga
     4441 aggaacaatt actttagatg atgccatgca agtgacaggt agcttaactg acgaaatgat
     4501 ggacagttac ttgagtgctt gcgtgaaaaa agaagtacca gaagcattgg aaacattgaa
     4561 ccaaattctc gcagccggca aagaatcacg tcgctttcta gaggatatgt tacaatactg
     4621 ccgtgacttg ctgatgtatc agcaagcacc taacctagtt gaggaacaat cgagtacgat
     4681 cagaagtaca tttacagaat tggcacaaac tgtttcagcg gatcagctgt tttcaatgat
     4741 aaagattttg agcgatactc aaaatgaaat tcgttttacc aatagtgccg ctatttattt
     4801 ggaagtcgct acagtcaaac ttgcacagct accgtctgct tctgttcctt cagcagatgt
     4861 agcatttgca gatgatcaga tagtaaaaca gctacaacaa gagattaagg aactaagaaa
     4921 cgaaatcaag gaactacgca gtaatacagg aattgagaat acaaaagtac agccggtaca
     4981 gcaaacaaat cataaaaaac agacaagtac ctatcgtgta ccgacggaac gagtatataa
     5041 agttctgcgg gaagcgacaa gacagcatct gaaccaagta aaagaagtat gggaagactt
     5101 gctgatgagt ctatctgtca ctcagcgagc aatgcttaaa gcaagcgaac cagttgcagc
     5161 tggacctaca ggactagtga ttgcgtttga ttatgagatt gtttgtcaaa gagcagcaaa
     5221 tgacgaagaa ttgcagcttt cggtacacaa caacttaagc cgaatgatca aagattatgc
     5281 gcctgatcct gtttatatta caagagaaag ctggccagaa cttcgaagaa attatttgac
     5341 gcaggcacaa gaagagggaa cagaaaactc ttttgcttct gaagactcag aaggagaaat
     5401 cgagttgctt cctcaagaac aagaagtacc agaagaaaca cttgtagtaa aagcagagga
     5461 actgtttggc tccgaagctg ttacagtggt agatgattaa taaaaaggag tagataatta
     5521 tgatgcgtgg aatgggcaat atgcaaggca tgatgaaaca agtacaaaaa atgcaaaaag
     5581 aaatggcaga agcacaagag ctgctaaata aaacagagtt tacaggtaaa gccaccaatg
     5641 aattggtcaa agcggttttt acaggcgatc gtcgcatgaa agatatccaa atcaatgaag
     5701 aagtcgttga tcctgatgat atcgagatgc ttcaggatct tgtcgtaatg gcagtcaatg
     5761 atgcgatcgc acaaattgat gtggaaactg agaaaaaatt agggaaatac gcaaaaggtt
     5821 tgccaggatt ttagttttaa actgaaggag taatgaatat gcaatatcca gaaccaattg
     5881 cgaaactgat cgacagctac atgaaacttc caggaatcgg tcagaaaaca gcgacaagac
     5941 tcgcctttta tacgatcgat atgaaagatg aagtagtaaa tgaatttgct aaatctttgt
     6001 taagtgtaaa acgcgatttg catttttgta gtatctgcgg aaacatcaca gaagaagatc
     6061 cctgcgaaat atgtagaaat ccttcacgtg atcgaagcgt tatcctagtg gtcgaagagc
     6121 cgaaagatgt aatggcaatg gaaaaaatgc gagaatatca cggactttat catgttctcc
     6181 atggtgtgtt atcaccgatg gaagggacag gtccagaaga catcaatatt tcttctctgt
     6241 tgcagcggct gcatgatgca gatgtaaaag aagtgattat cgcaacaaat gcaacaacag
     6301 agggagaagc aacagctatg tatctctcac gtctgatcaa acccgcaggt atcaaagtga
     6361 ctcgtctagc acatggtctg tctgtaggct ctgatatcga gtatgcagat gaagttactt
     6421 tattgaaagc tgtcgaagga agacgtgagt tataaatttc taattaagtg ttttttcaga
     6481 tacaatataa aagcagcaca agagatgggg gcaacaatgt gaacggacta tttattacaa
     6541 tcgaggggcc tgatggtgca ggaaaaacca gcatcttgaa tgaactttac cctcttttga
     6601 aagaagtagc aaaaacagaa atcattcaaa caagagaacc aggaggcatt ccgattgctg
     6661 aagagatccg agcagtgatc cttgatccaa aaaatgatcg gatggatgaa cgtacggaag
     6721 cactgcttta tgcagcagca agaagacaac atctagtcga aaaggttctg cctgctctgg
     6781 caaaagggaa aatcgtttta tgcgatagat ttgtcgacag ttctcttgct tatcaaggtg
     6841 caggccgacg catcggcgtt accgagattg ctcggttgaa tgagtttgct acagaaggta
     6901 ctacgccgga ttttaccctt tatcttgacg tagattcaga caccggatta agaagaatca
     6961 agaaaaacag acaaaatcaa atcgaccgtt tagattcgga aggtttggaa ttccaccagc
     7021 gtgtacgtca tgcttactta aagctggcag aagagaatcc ggaaagaatc cacaaagttg
     7081 atgcaagaaa aagctttgaa gaagtgctgc aaacaagtta tcatacaatt attgaacagt
     7141 atccgcaatt ttttgaaaac taggaaggtg aacgtttatg aaaattattt tagcgattat
     7201 ccaagacaaa gacagcaacc gtttatcaaa tgaattgatt gatgcaaata tccgtgccac
     7261 aaaactatca tcgacaggcg gcttccttaa ggctggaaac agtacgttta tcgtgggtat
     7321 tgaagatgac cgtgtagaag aagcattgga aatcatcaaa aaaacgtgtg aatcaagaaa
     7381 gcaatttgta tctacacctg ttacactgga tatttcgatg gatgggggcg tcccttatcc
     7441 agtagaagta gaagtaggcg gtgccacagt cttcgtctta cctgttgaag gattccatca
     7501 attttaggag gagctgagct atgaatcaag aaataaactt ggctcaaatg caaccgatcg
     7561 tgtatcagca gcttcaacgg agttttgagc atgggcgtat cgctcatgct tatctttttg
     7621 aaggggagaa aggaacagga aaacatgaag taggtatatg gcttgcacag cacctgttct
     7681 gtacccaaat gaaagacaat ctgccttgtg gaaaatgcaa caattgtcag cggatcgaaa
     7741 agaaagaaca tccagatgtt ttggttattg aaccggaagg acaaaccatc aaagtcgatc
     7801 agatcagacg tttgcagacg gaattcagtc gcagcggtta cgaatcaaga aaaaaagttt
     7861 ttttgatcaa agaagctgaa aaaatgaata gcagtgcggc aaacagtttg ttgaaattct
     7921 tggaagagcc ccctggagat tttttagcgg tgttagaaac agatgcagta ggaagaattc
     7981 tccctactat tcaatctcgt tgtcaagttc tgcattttca agaactttcg aaagaagcac
     8041 tgatccatcg attgcaagaa gagcagattc cattggaaaa agcaaaactt ttggctttct
     8101 taacaaacag tttaggaaaa gcagttgaaa tctctcaaga tgaatggttt aatgatgcta
     8161 aggacttgat ttatcaatgg ttcgtttact tgcaaaagca agatactcaa gcctttatct
     8221 atgtccaaaa aaaattggta aaatcattta aagaaaaaac acaacagttt acaggattat
     8281 ctgtcttgat gttttattat caggaagcac agcgtcaagc agtagcaaac ggcgaatatg
     8341 gcaaagtgaa acagataaac cagataattg aacggatatt gatcgcagag cagaaactac
     8401 gcagcaatgt cagttttcag gctgttgcgg aacagttcgt tttgcatacg attttttagc
     8461 ttgccgcaaa ggatgatgat caaggaggat ggaaatggta gaagtagtag gtgtccgata
     8521 tagagatgca ggtcatatct attattttgc accaggagac tctgaatatg tttatagtga
     8581 caaggtatta gtcgaatctc agcaatcctt acatattgct actgttgtcc ttcctaaaaa
     8641 agagattgat cccaatgacc ttcctgaagc tatccgtccg atcttgcata aagcgtcaca
     8701 agctgaatta gaaaaagaca aaaaaaacaa aaaagatgct tatgatgcat tgaaaatcgc
     8761 aaaagaaaaa gtgcagttgc gtgagttgga aatgaaaatg gtcaaagccg aatacacatt
     8821 tgatcgaaat aaattgattt tttctttcac agcggacggg cgaattgact ttagagaatt
     8881 ggtaaaagat ctggccgctg tatttcgtac tcgtattgag cttcgacaaa ttggtgtccg
     8941 cgatgaagca aagataatag gcggaattgg tccatgtggg cgtccattat gctgttcttc
     9001 ttttttaggc gatttcgtgc ctgtatcaat caaaatggca aaagatcaaa gtttatcttt
     9061 gaatccaaca aaaatttccg gtttatgcgg acgcttgatg tgctgtctgc aatatgagag
     9121 cgaagaatac gagacagcga aaaaagaact ccctgacttt ggaaaagtaa ttgctacacc
     9181 tgatggtaaa gggaaagtcg tagggttgaa tctgcttagc cgtatcgtta gtattcggct
     9241 gactggacgg gaaaatcctg tcgaatatga atgggatgaa ttgaaagaat ttatccaagt
     9301 aggtgagatg aatggataag agatcattat acgacggatt gaatcaatta gaatcagagt
     9361 tgagaaatac attaacagaa cttgttgaaa tgaagcatgc tttgcatgag atcgtagaaa
     9421 aaaatacaac tttggagatg gaaaatcaac gtctgcggga acacctgcgg gaattgaacc
     9481 aagttgcaca agctccagct gaaaatgtga agcaggaact ttctaagtct cgtatgaatc
     9541 ttgaaaaaat ttatgaagaa ggattccatg tctgctacga tttatatgga tcaagaaggg
     9601 acaatgacga gccttgtgcg ttttgtctag aagtgatcta tcgcgaaagc ggtcgatgac
     9661 caataacaag aggagactgg agcggagaaa tactgctcca gtctttttat taaggagatt
     9721 gtgacagatg tataatcaaa aaagtttcaa gggaaatccg gtatatgggg atttatatct
     9781 tgtaccgacg ccaattggaa atttagaaga tatgagctac agaagtgttc gtatgctgca
     9841 agaatccgat cttatcgcaa gtgaggatac gcgtaataca caaaagttat tgaatcattt
     9901 tgaaatccat acaccccaaa aaagtttaca tgaacataat tataaagaac gtgtccctca
     9961 attgattgct ttgttaaaag aaggaaaaac gattgctcaa gtcagtgacg caggaatgcc
    10021 ttccatcagc gatcctggac atgagctagt tttagcctgc ataaaagaag ggatttctgt
    10081 gatctctatc ccaggaccta cagctgggat gacggctttg attgcttcag ggctagtgcc
    10141 tcaaccattt ttattttatg gcttccttcc acgtaagaaa aaagaacaga aagaaacatt
    10201 gaaaaagcta aaagaacaga cagttacgct gattttttat gagtcacctt atcggatcac
    10261 tgctacattg acgaatttct tagaaatttt tggaaatcgc caagtcgttt tgtgtcggga
    10321 attgacaaag gtccatgaag aataccttag aggaagtacg gaagagctac tcgcttatat
    10381 cgaagaacat ccagtaaagg gtgaatgttg tctgcttgta gaagggaatt tgttcgaaat
    10441 gccagtgacg gaagttgatg aagagttggg aacgttaaag gaacaagtag aagcaaagat
    10501 cgcttcaggc gaaaaaccaa atgctgctat caaagcagta gcgctgaaaa atgggctgaa
    10561 aaaacaagaa gtctaccgtc agtaccatga attagactga gatagtataa gttgtctata
    10621 atgaaagact aatgaggaaa tcagctacta acttttcaaa tctcttgaac gcgaacgaat
    10681 gttcttgtat aatgaataag aggtgaggat gatggagctg caatttcctt tacataatga
    10741 tacatcaaga aaaatcattc atattgatat ggatgctttt ttcgcttcgg ttgaagaacg
    10801 agatcatcct gagtttcgcg gacatccatt agtaattgcc agacacccca gtgacactgg
    10861 tgggcgtgga gtagtgacta cagcaaatta tgaagcaaga aaattcggaa ttcattcagc
    10921 aatgagtgca caaaaagcgt atgagctttg tccgcaagcc atctttaagc ctggcgacca
    10981 tcataaatac agcgatattt ccaaacaagt acgggaagta ttctatcgtt atacagatat
    11041 tgtggaacca gttagtatcg atgaagcgta tcttgatgtg accgaaaata aaatcaattg
    11101 caagtcagcc atcaaaatcg cccgaatgat ccaagcagat atttgggaga gtgtccagct
    11161 aacatgttct gcgggagtaa gttacaataa gtttttggcc aaattagcat cagactatca
    11221 aaaacctaga ggaatcacgg tagttgcccc tcaagacgct gtgccatttt taaaagcttt
    11281 gccaattgaa aaatttcatg gggtagggaa gaaaactgta cctagaatgc atgagctagg
    11341 gatttataca ggaaaagacc tatatgaatg cacagaaatg atgttgattc gaaattttgg
    11401 aaaaatgggc tattctcttt atcgtaaagt cagaggaatc catgattctc cagtaaacgt
    11461 tacaagagag cgaaaatctg tgggaaaaga gcatacttat gggcagccgt tgcaaacgga
    11521 agaagcagtg ctgactcagt tgcgccaact agctgaaaaa gtagaagaag cactgagaag
    11581 agtccaaaaa catgggaaaa cagtagtttt gaaggtcagg tacacggact attctactgt
    11641 gaccaaaaga gtgacattgc cagaatatat ctataaaaaa gaagcactct tttatcaagc
    11701 aagccttata tgggaggaaa tcttgggagt agaaaaagga atccgattgt tagggattac
    11761 tttgacgaat ctggacccga tgacttatga aaacatcgtc cttccattat gggaaaatca
    11821 agaaatataa aaacagcttt gagagttgaa accggaaatc cgttttctct cccaaagctg
    11881 tttttatatc aagtcttgct ttttgatttg ttcataggtt tctgttgcat ccgtacattt
    11941 ttcccaaatc acaacttgat tggattctaa agatttttgg acgtcaatga tccgctggtt
    12001 gctacttccg cgaaattgta gttttaggtc acgtttgctt aattcaaagc gtccgtcaac
    12061 aaggatatcg atttgcgaaa ggagttctaa tttatcttct gaatcttgta gcaattcatc
    12121 aaaggtatag cctgtccatg accagatatc ttttgtcgta ccgaactctt ttcggatacg
    12181 tcgtacgact tgcagacaaa catctgtatt caaaaaaggt tcgccaccaa gtaaagttaa
    12241 tccttgcacg taatcgttgc gcaagtcagc gatgatttgt tcttctaatt caggtgtgaa
    12301 tggtttgcca tatcggaaat tttgtgcagc tttgttgaag cagccttcgc aggcaaataa
    12361 gcagccacta acgtaaagac tgttgcggac accttcacca tcgacaaaat taaacggttt
    12421 gtagtcagca atatagtttt ggctgtagtc ttttgctaac cattcttttg gttttggatt
    12481 tctcataaaa aaacttcttt catttaaggt tcaaagatat agctattgaa gataaaatct
    12541 actcgaccac ataaataaga acaaactgac ctaaatccat tgttcaagaa agcttgttct
    12601 tattttgata gagggcgttc aaagatacaa agccatcgaa agagggtggt gaaaaaagac
    12661 ttgtcatagc ctcttattct gaataaacgg tgttcaaaag cttgcatctg cagtaataag
    12721 cccaactaaa cgaaaaatat gaggaactat ttttgtttag ctattcttat tacttgatgc
    12781 aggacagctt tttcacaacc tcatttcatg tgtttcacac gtgaggaaat ttctttatgg
    12841 cggccatgta ccatcggacg agcttgcgga tttcctaaat agccacaagt acgtttgact
    12901 acatcacatg tttttggatc atgatttcca cattctggac attcgaaccc tcgttctgtc
    12961 ggtttgaaat caccttcgaa accacattga tagcaatgat cgattggcgt attcgtacca
    13021 agatacccga ttttgtcata tgcaaaatcc catacagctt ctaaagcttt cggattttgt
    13081 tggagcatag ggtattcaca gtagtggatg aatccacctg aacaatattt tggataatcc
    13141 ttctcaaatt ccaatttttc aaatggcgtt gggtttttac gcacatcata gtggaaactg
    13201 tttgtataat attctttatc tgtgatattt tcgatgatgc cgaatttttc tgtatccaaa
    13261 cggcagaaac ggtcagttag actttcactt ggagttgaat agacactgaa atgataacca
    13321 tattcatccc cccatgcatc tgcattagct tttaattctt tgacgatatc aagtgtgaaa
    13381 gcttttgctt ctgggtttgt ttcccattcg ccaccgaaga aagctgaagc aacttcatac
    13441 aatccaatgt agcctaacga tacagtagct cgacggtttt tgaataattc attgacagaa
    13501 tcggtacgtg aaagacgttt cccgaaagca ccatacatat ataaaattgg cgcatttgct
    13561 ggaatggctt ctttacaacg ttctacacga tagagaagcg catctttcat gatttctaag
    13621 cggtcagaca gtaactgcca gaatttattc tgatctcctt ttgattcgat cgcgattctt
    13681 ggcaaattca atgtaacaac acctaaattc attcgtccga catttacttc ttgaccattt
    13741 tcgtctttcc acccttgcaa gaatgaacga cagcccatag gtactttgaa acttccagtt
    13801 aattcaacga ttttatcgta attcagaata tcaggataca ttcttttcgt tgcacattcc
    13861 aatgctagtt gtttgatgtc gtaattagga tcttcagggt ttaagttcac accttttttc
    13921 aaagtgaaga tcagtttagg gaagatagct gtccgctttt cactccctaa tccattgatt
    13981 cgaatctgta aaattgcttt ttgaatttct ctttcaaacc agttttctcc tagtccaaat
    14041 cctaatgaag tgaaaggagt ttgtccattt gaagtaaata aggtattgat ttcatattcc
    14101 aaactttgca tagcatcgaa gatatctttt ttggtttttg cttttgcata ttctttttgg
    14161 cgttctggcg aatcgatcca ttcttccgcg tcttttagat gttttttata gttcaattct
    14221 gcaaaaggag caagcaattc atctgttcgg tcggcagaac agccaccata ttgacttgac
    14281 gctacattgg caataatctg tgagatttgt gctgtggcgg tctggataga ttttggcgat
    14341 tcgacttcgg cattgccgat tttgaaccca ttgttcagca tgcctttgaa atcaatcaag
    14401 cagcaattcg tcatcggtgt ataaggatga taatccaaat catggtaatg gatatcgcct
    14461 ttttgatgcg catttgctat atgtggtggc aacattttca gtccgattga ttttccaacg
    14521 atcccggctg tcagatctcg ctgtgtattg aaaacattac tgtctttatt tgcgttttcg
    14581 ttcacgacag tttggtcctt gttgattagc ttttcaatcg aaaagttgat atctgttgct
    14641 ttactgcgag caaaatctcg tttggtacga taattgatgt actcttctgc taattcatat
    14701 tcgttttttt ctagtaatat atgttccacg atgttttgaa tctcatagat tttgacattt
    14761 tcagcaaacc ggcttttgat ttcttggaca accaagtctg caatcatttg aattttttca
    14821 taagtaagtg gtgataatga gccgtgaatt ttcttttctg ctttgatcag tgcttcattg
    14881 atcttttgtt catcgaaatc aacatggcgg ccatcgcgtt tgatgacttt catctgggat
    14941 gtctctggct gctgagtaac gacagttttt tcttcataat ttactttcat tatcttcaac
    15001 tcctgctaaa ttgttcttgt ctatcataaa acaaaatcgg ggaaggagca actaaatatc
    15061 ggctatatat agtattcaat gtgctgtata attggatttc tatactatat gtagtgtata
    15121 ttttattgtg aaaaaaggtg aaaacttgta gctggtggat ttgttttttg catatttaat
    15181 tttgctgaat aaaataattg aataaaattt tttattcagt ccaagtaaaa gtgacaaaaa
    15241 cataagagca aattattcgt agatatgcta ctatgcaaat aaatagaaat gaaaaatgaa
    15301 agaggaggag aagaattgga aaagatgatc gaagtgaaac atctaagtaa aagctacggc
    15361 agcaaacttt ctcccgtcaa agccttagat aatatctcat tcacagtaga agaaggcgag
    15421 tttgttggga ttatgggccc aagtggtgca ggaaagacaa cgctgatgaa tatcttgtcc
    15481 acgatcaatt tgcctacgat gggcagtgtc tcgatccaag gaaaagaaat cacaaaaatg
    15541 aaaaacagtg agctcagtga ctttcgtcga aaaaaactag gattcatttt tcaagagttc
    15601 aatttgatcg ataccctaaa tgcaaaagac aatatcctat tgcccctagc agttgaacgg
    15661 atgactaaag aggaaatgga aaagagagta agacatgtag ctcaattgtt gaatatagaa
    15721 gaactgctca aacggtatcc cgatgaactt tctgtcggac agagacaacg aattgcagct
    15781 gctagagcac ttgttgttca gccacaagtg atttttgcag atgaaccaac tggctctttg
    15841 gactcgaagt cggctacaga attgttgaac tacttgaaga ccatgaatca aaaagaaaaa
    15901 gcaacgatac tgttagtcac ccatgatcca tatacagcaa gctactgcga tagaatttta
    15961 tttattaaag atggtgtgat tttttctgaa gttgtcagaa gagggacaag aagagagttc
    16021 tttgagaaag taatagacat gcaggcgacg attggcggag gaggaaaaat gaatgctgta
    16081 taaactagcc gttaaaagtt tttttcggca gtcacgaggc tatcttgttt attttttcag
    16141 tttgacttta tccgtaatga tctattattc cttcagcgca atgacctacg atcaatcttt
    16201 agtcagaaga gcaagtcaag atacttcgat agatggtgga ttgagttttg gtggatttat
    16261 cattactgtt gttttgcttt ttttcgtatt cagtgccaac cgatttttct tgaatagaag
    16321 gcaaaaggaa ataggcatct atcagctttt tggtatgaat aaactgcaga tatcaggtat
    16381 atatgtgtta gaaatcatga taattggatt gttcgcctgt atttctggga tattactcgg
    16441 tattattttt tccaaactct tttcaatgat tttggttcga atgatggata tggatcttac
    16501 tagtccattt tttatctcca ttccatcagt agtagatact ttatttgctt ttttcatcat
    16561 ccttttagca gtatctgtat attcaatatg gaaaatatgg cgctatccaa tgattcgaag
    16621 ttttggagaa aaagaacaag cagatagttc aacaatgcgc ttcaggacaa ggcaccgatt
    16681 actcgcagtc atcgggttac tacttttaac gagcggatat tttggagcta gtcattttag
    16741 ggaaatcact tcatggatga tcagttatca ttatcttgat acattagttt tcttttttcc
    16801 ttttttaatt tttttcgttt gtgtggtcgg tacttatttg tttttctgtt atacatttcg
    16861 gttatttttg atcatattga gtaaatggaa gatttattac tatggtatca attttttgat
    16921 aatgggtaac acgcaagttc atttgctcaa aagttggcgt acaaattctc tgattacagt
    16981 cattctcgga atttctttaa tgacaattgg cggcacggct agtatctcta ctattttatc
    17041 ctaccaggag aaagtttcct ccccgatgga ctatcagttt gatgttgaga cagagaaaaa
    17101 aatcaagccg attctagcag aggaagggca gaagataact aaagaaatca ccttgcatta
    17161 taaagtcatt gggagtatct atagtcaaaa tctcgaagaa cctatgtttc aaactgctaa
    17221 tctgatcacg gaaacacaat atcaagcctt cagaaaagtt aatccagatt tgccgaacat
    17281 ttctttgaaa tcacctggtc atactgtttt gcttgatcaa atgcaaactc tttttcggaa
    17341 tatttcatta tatggatcta gtattggact gccagataat caagaattga agattcaaca
    17401 aatgcttcca agtgtattag gtgacgaaac aatggtatat ctaagtcctg tactgattgt
    17461 agatgatacc gtttttgaaa aagcaaaagg tgtggattat caagtagtca atgtagatgt
    17521 atcaggtgga aataatgaaa aaatagatga acgactgagc aatgacctgt cgataaagtg
    17581 gctcaatgct gtttattatt cttatgatat cgtaaatgga agtctacaag gagaaattag
    17641 cacaaagaaa cttccggatg accaaatgaa ggaagcacaa tttagccaag aaagctctcg
    17701 tttgaatttc tcttccagct attcgaaatt gcgtgcagtt aatcggcgaa tcggtatcaa
    17761 tatttttgtc acactatttg tcgggatgat cgtgattatt gctactggtg gtagtatttt
    17821 gacagtccgg caattagcag aagcagagga agaaaaagaa aattatcacc tattgactaa
    17881 gctggggatt ccacaaagaa ggatcagacg gatgatcttc gagcaaaacg cacttacctt
    17941 ctttccaccc atgatattag ggatcactca cgcagtattt gcaatcaatg tcttcactca
    18001 gtatataaaa actgccgatt actggttggc ttatcttgtg agtggcttat tgatccttat
    18061 ctatcttttg ctttatgtca ttacgagtca gttatattgt cggattatcg aggaatgata
    18121 tacggaataa gacaagataa taaatagaac taaattttga ataccattta tttggaataa
    18181 gaggttgtga cacgattttt gtcacaacct ctttattttt tatatttttt aaaagtacga
    18241 gaaattataa agaaaaaagc agtgatttga tgtgctattt ttataaaagc tatgtgacaa
    18301 aactgttagt aaaaaaacgg tttctgtaag ctagacacat gtttcttttt gtcaaaatta
    18361 gttacacttt gtgtgaggtt atatgaggag ggaatccaat gaacaaatgg cgttctaatc
    18421 caaaattgaa ttattgggga aaattcatca ttcgaacaat tttttatagt gcagtactgc
    18481 tgttcctgat ttatctgtat cactataaaa atattcaagg aggaactttt atctataacg
    18541 aattttagga ggaagagtat ggaaatcaaa acgattattg aagcaatcga tgattgggga
    18601 ataaatgaac ctgatcgagt tgcttatcag gcggatgaga cgcatacttt tggtgaattg
    18661 aaacaagcat cagacgcact tgcttattat ttagaaaaaa aggtagaagg cgatggacca
    18721 attgtcgttt ttggcaattt ggagtttgaa atgattgtct catttctagg ggttgtcaaa
    18781 tctggccatg cctatttgcc gattgaagag catacaccca aggagcgtat cctatcgatc
    18841 tttcgggtgg ctaaaccatc tatggtcatc agtatcgggg actggataga agatattcct
    18901 tcggttcctg tcataacaaa aaaagaattt caagaaatca agaagatacc agtaggattt
    18961 tatcccagaa atagcgtaaa aggtacagaa aatttttata ttatttttac ttctggtact
    19021 actggagaac caaaaggcgt acaaatcagt catgataatc tagtcagctt tgttcaatgg
    19081 acgatggaag actttgggat caagcctggt atgcatttct tagcccaagc gccattttct
    19141 tttgatttat cggtattttc tatttatcct gcattagttt ctggcggaat gctgaagccg
    19201 ttaaataaag cagttgttca agactttcgt caattattcg cgacattgcc agccttgaag
    19261 ttgaatgtgt gggtttctac tccatcattt atggatattt gcttgatgga acctacattc
    19321 aatggagaaa atgtgccgga attggacatg tttttatttt gcggagaaga attgacgaaa
    19381 aaaactgcag aaagtctact tgatcgattc ccaaatgcac ggatatataa tacttacggt
    19441 ccaacagaag caactgtagc tatttcaagt attcagattg accagcatgt actagatagt
    19501 tatgaccgac taccgattgg ctacgtaaaa aaagacacga aagtttctat tgtccaagaa
    19561 ggtaaacctg tttcaaaagg agaaacgggt gaaatcatta ttgcaggacc aagcgtatca
    19621 aaaggttacc tgaacaatcc cgaaaagaca gctgctgcgt tcttttgtca aaatgactta
    19681 gcaagttacc atacagggga tgcaggtaga ctagatgaat atgggctttt attttatgaa
    19741 ggacgtatgg atcagcaggt caaattacat ggataccgga ttgaactcgg ggatatcgaa
    19801 cattatctgc tccaagacaa ccgaatcaaa caagctgtcg tcgtaccaaa ataccaagga
    19861 acaaaagtac agcagctggt tgcttttgtc gtattagaaa acaaaacgaa acagccggat
    19921 tttcagttga ctaaatctat caaagaacaa ttactagaag ttgtaatgga ttatatgatt
    19981 cctcagaagt tccattatgt cgatctgctg cctcagacag ttaatggaaa aatcgatcga
    20041 aaaaaattaa cagctgaggt gaatcctgaa tgatgttacc tcatatgatc ccttatgcag
    20101 agccgtttta tttttttctt ttagccattg cgcttgtacc gatcgttcta tctttattga
    20161 tatgggaaaa aagattacca gtttatcaat cagcagtgac acttttcttt ttatttgtca
    20221 gctttggagg taattattgg caacagggag cggcgctgat tgggtatgtt ttgtggcaga
    20281 cgatacttgt atggggctat tttgtttatc ggcaaaggaa aaatcagaca ggatggtttt
    20341 ttgccgccgt tattttagcg ataattccac tgtttttaac gaaagtgact cctttttttg
    20401 aacatggaaa agcatcgtta cttggttttt taggaatctc ttatttaacc tttaaatctg
    20461 tacaaatgat catggaaata agagatggca tgatcaaaac atataagcca tttcgttata
    20521 tccagtttct gctgttcttt ccaacaatct cttctggacc aatcgatcgc tataggagat
    20581 ttgaaaaaga tcagctccat ccgcctgttc gtgataaata tgttgatctt ttgggaaaag
    20641 gcgtccaaaa tatttttgtc ggttttttat ataaattcat tatcggttat tattttggtc
    20701 aagtactgct tcctgtagtg ggacgcattg ccttacagca tggtggtatt tcatgggcat
    20761 tagtaggtta tatgtacgtg tacagtatgt atttgttttt tgattttgct ggctatagct
    20821 tatttgcagt cggtacaagc tacatgatgg gatatcagac accaatcaat ttcaataaac
    20881 cttttttatc atggaatatc aaagaattct ggaatcgttg gcatatgacg ctgtcctttt
    20941 ggttccgtga ttatgtatat atgcgattga tgttcttcat gatgaagaaa aaatttttta
    21001 aaagcaagat cgtaatgtca aatatcggtt attttgcttt gtttcttcta atgggtatat
    21061 ggcatggatt gacgtggtac tacatcgtat atggactgta tcatgcttgt ctgatctgtc
    21121 tgacagatgc atggatccga tttaagaaga agcataaaga gcagttgcct tctaacaaat
    21181 ttacgcatgg cttagccgta tttttgacat ttcaagcagt atgttttagc ttcctgattt
    21241 tttcaggatt tttagataaa ctttttttta gctaacgaat agtgacatat taatttatat
    21301 aacaaaggag aaaaggaaaa tggaagaaca aattttatca atcttagaag aaataacagg
    21361 aacagatgaa gtaagaaaaa atcgtgatat tgatctattt gatgaagggt tgcttgattc
    21421 tttagcatct gttcaattat tagtcgagct agacggagag ctggatatcc aagtccctgt
    21481 atctgaattc gagcgagagg attggagcac accgaataaa atcattcaac aagcaactgc
    21541 attgaagggt tgagtatgaa aaagaaactt tttgctattt tcggcccggt tgttttagct
    21601 gttggcttac tgtttgtgtt ttttacctcc tcctatcaag tagatatctc taatccagaa
    21661 acgattagac gtgcctcttc ttccatgtca gtgaacgtgt tgaaaggaga tgcgataaaa
    21721 aattatgcat tatctgaaaa acaatatatt cctttttttg gctcaagtga attaagcaga
    21781 atcagtccat ttcatccgtc tgttctggca gaaaaatacc agcgaaacta tcgtccattt
    21841 ttgctgggag cacccgggac acaatcgtta agccaatata tgatgatgcg ttctgcagga
    21901 gatgctatga aaaacaagaa agtcgtcttt attatttctc ctcagtggtt cgttaaaaat
    21961 ggtgtaaaaa ctgattactt taatacgtat tattctgaac ttcaaacata cgactggttg
    22021 ttttcaatga agaaagtaac gcctgctgat cgctatttag ctagaagatt attaactttc
    22081 tcaaaagtaa aagagaatga tacgttgacg gcgattctgc aaacgatcaa aaaagggaag
    22141 ctacctttac ctgaatcttt gaatcagctg cgcagccaat ggaacatgct gaagagagag
    22201 gatgaagtat tcagcaatat tgggttgaaa gatcgacagc aaaaaatcga tcatgagtca
    22261 aaacgattac caaaacaata ccaagaaaca gaattatcca ttttagcaaa tcaaattgga
    22321 gaacgagaaa cgacgaataa tccattcggg ttgaaaaacg atttttatac gcatcgtatc
    22381 cgagcccacg agccagagct caaacagtca caaaaaaatt gggattatcg tttttcgcca
    22441 gaattttctg attttcagct ggtcttggat caattagcaa aaaaccataa cgaagtattg
    22501 ttcatcatcc caccggttaa cgagaaatgg agcgattata ccggactatc tcaagaaatg
    22561 ttacaaggat ttgccaaaaa gatcaaattt caattaaata gccaaggatt caatcgaata
    22621 gctgattttg tcaatcaagc gggtaccaac tattttatgg aagacacgat ccatctaggc
    22681 tggaaaggct ggttggctgc agatcaacag atcagaccgt ttttagaaga aaatcatatt
    22741 actgctagta agtatcatct tgacgatgct ttttttagca aatcttggca acatcaaata
    22801 ccagataaac tacaattaaa ataaagaaaa caggcgagtg ttgctcgctt gttttcttta
    22861 tttttgaata ttctgaataa aagataaaaa
//
LOCUS       DACEPD010000046        22055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.047, whole genome shotgun sequence.
ACCESSION   DACEPD010000046 DACEPD010000000
VERSION     DACEPD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.047"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(452..652)
                     /locus_tag="IW797_001621"
     CDS             complement(452..652)
                     /locus_tag="IW797_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1224404.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(957..2189)
                     /locus_tag="IW797_001622"
     CDS             complement(957..2189)
                     /locus_tag="IW797_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1224405.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2443..3012
                     /locus_tag="IW797_001623"
     CDS             2443..3012
                     /locus_tag="IW797_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1224406.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3190..3630)
                     /locus_tag="IW797_001624"
     CDS             complement(3190..3630)
                     /locus_tag="IW797_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ1224407.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3788..4552
                     /gene="map"
                     /locus_tag="IW797_001625"
     CDS             3788..4552
                     /gene="map"
                     /locus_tag="IW797_001625"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ1224408.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4584..5507
                     /locus_tag="IW797_001626"
     CDS             4584..5507
                     /locus_tag="IW797_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ1224409.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5583..6722
                     /locus_tag="IW797_001627"
     CDS             5583..6722
                     /locus_tag="IW797_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ1224410.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6715..7515
                     /locus_tag="IW797_001628"
     CDS             6715..7515
                     /locus_tag="IW797_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1224411.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7515..8342
                     /locus_tag="IW797_001629"
     CDS             7515..8342
                     /locus_tag="IW797_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1224412.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8320..9054
                     /locus_tag="IW797_001630"
     CDS             8320..9054
                     /locus_tag="IW797_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1224413.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9154..10020
                     /gene="rfbA"
                     /locus_tag="IW797_001631"
     CDS             9154..10020
                     /gene="rfbA"
                     /locus_tag="IW797_001631"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ1224414.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            10034..10606
                     /gene="rfbC"
                     /locus_tag="IW797_001632"
     CDS             10034..10606
                     /gene="rfbC"
                     /locus_tag="IW797_001632"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ1224415.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10628..11656
                     /gene="rfbB"
                     /locus_tag="IW797_001633"
     CDS             10628..11656
                     /gene="rfbB"
                     /locus_tag="IW797_001633"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ1224416.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11754..12605
                     /gene="rfbD"
                     /locus_tag="IW797_001634"
     CDS             11754..12605
                     /gene="rfbD"
                     /locus_tag="IW797_001634"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ1224417.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12640..14673
                     /locus_tag="IW797_001635"
     CDS             12640..14673
                     /locus_tag="IW797_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224418.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14717..15997
                     /locus_tag="IW797_001636"
     CDS             14717..15997
                     /locus_tag="IW797_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ1224419.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16207..17013
                     /locus_tag="IW797_001637"
     CDS             16207..17013
                     /locus_tag="IW797_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1224420.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            17025..18245
                     /locus_tag="IW797_001638"
     CDS             17025..18245
                     /locus_tag="IW797_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224421.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18235..19821
                     /locus_tag="IW797_001639"
     CDS             18235..19821
                     /locus_tag="IW797_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1224422.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19860..21998
                     /locus_tag="IW797_001640"
     CDS             19860..21998
                     /locus_tag="IW797_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1224423.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6964 a         3732 c         4570 g         6789 t
ORIGIN      
        1 aaaaagtata cacccttctt cattctagtt ttcgcagcag gaattttgaa atagttctta
       61 ttagaatagt agagattgat taataagcta tcttcgattg ctaaaaagaa aacttctgaa
      121 ttccatactt tataaaaatc tttgataaat ctattcgaag ggtcaaattt aaaccataat
      181 tgtgtttttc cttccatcag cataaaagtt cacctcaaaa agagtatacg aacgaatgtt
      241 ctttttgtga aggcggaatt taatatactg aataatagaa aacaagtact tgtgccaaat
      301 tatgtgccaa aaaaattcga attcaacaaa atgtaaacaa atagaaaacc ttctatatta
      361 agatttcctg caataacaaa cacaacagaa gacgtgtaat agttagtcag gaacgtacaa
      421 ataacccctg tatcctttgc ggtacagggg gttattttgt ataagtagac attgtagttt
      481 ctaaactgct atgtcctaaa cgtttcgata cgctaagtat atttactcct tgataaagta
      541 aaatagatgc atgcgtatgt cttagtccat caataaacag tcgaagagaa aatagggaaa
      601 aattgattac taaagaaaaa cttactaggt cgacggtgta gttgattttc atacttcaat
      661 agattcgaat taaacgattt cgattggctt ataccagtcc tatccaacga aagatatatc
      721 gttcttaact ctagcggtat aacttgaagc tttacgtaaa gccaatggga atgaactgga
      781 gatgactttt tattattttt agtaatagct gatgggttaa tacttacaac tttccttttc
      841 aaagtaccat ataaaaaaac ggtggggttg tttagcgaaa taaaggatct aagccaatag
      901 cttagatcct ttattctatg ctttatatta ttttttgcat gtgtgcgaaa gtatactcaa
      961 gcccaatcgc catttcggaa aatcggtact gtagagccat cttcacggat tccgtcgata
     1021 tccatcttgt cagatcctac catgaaatct acgtgggttt gacttctatt caatccagct
     1081 tctgctaatt cttcatcgga catttctgta ccgcctttca cactgaaggc ataagcagaa
     1141 cctagtgcta agtgattcga tgcgttttca tcaaataatg tattgaaaaa gatgatgcca
     1201 gattgagaaa taggtgaagg gtcaggtaca agtgcgactt ctcctaaacg acgcgcacct
     1261 tcatctgtat caagcagttt tgctaagaca tcttctcctt gttcggcaga aaaatcaaca
     1321 acttttccat ctttaaaagt aaatttcata cccgagatga tggtgccagc ataactcagt
     1381 ggctttgtac ttgaaatgta accatctact cgatgactgt caggtgctgt gaacacttct
     1441 tccgtcggca tatttgccat gaatttttct ccacgagcat tatagcttcc agcgccttcc
     1501 caaagatggt ttttaggcaa tccgataatg atatctgtac cgggagcagt atagtgaagg
     1561 gcagaaaatt gttctcgatt caattcttct gctttttttg ctaatttttc atcatgcttc
     1621 ttccatgcta agacagggtc ttcttcatag acacgagtcg ttttgaaaat ctgatcccat
     1681 aatgcctcaa cttgtttttc ttctggcagt tctgggaaga cttttgctgc ccattgttta
     1741 ccagccgcag ccacgacagt ccaactgact ttatttgctt gagttgcctt gcgtaaattc
     1801 attagtgctt tgccattcgc tgattgataa gacgctactc gatggctatc tacaccagca
     1861 aatgcatcgg gatcggcaga aacaacgctg atcctgcttg ctcctttttc aagccattca
     1921 tccgcttgat cgattttaga ttgtggtaca ttctcaatac gatccgttgc agcatgtaaa
     1981 agaaattctc tttggatctg gtcgtcagtc cattgaacga taacctcagc ggctcctagt
     2041 ttataagctt cttgtgtgat caatcgagca aggggtgcct gttccacgct gatctgtaaa
     2101 acaactgtgt gtcctttttc tgtggctacg ccagtttctg caattaagcg tgcatatttt
     2161 tttagaagtt cattaaaatc agataacata ataaaaattc ctcctcacca ttttttctgg
     2221 taactattag catgatacca tttaattatg attcaaacaa tgaggcttta aataaaaaaa
     2281 ggaggttatt tttttaaggt taagtttcat gatatatgtt actacttgct atttcagctt
     2341 gtgaagaaaa tcaaaaacta attttgcata tcaaaaaaac atgttacaat gaaattaatc
     2401 aatggtggaa gcgttataaa aaaattaagg agattaaaaa atatggcaag aaaaaagacg
     2461 attactagag aacagatttt ggcagctgct tatgaagtag ctgccaccga tggatttaca
     2521 cgatttactg cgagaaacat cgcgaacaaa atgaattgtt ccacgcaacc gatttatctt
     2581 gagtttaaaa atatggatga cttgagacaa gctctttttg agcagatcta tgatcattta
     2641 caatatgaag tgttcactgt tgagcataca gggaatacga ttatcgatct tgcttttaat
     2701 tatatccatt ttgcaagaag agagaagaag ttgtatcgtt cactgtactt agaagaatat
     2761 ggtggaggaa aagagatgca gaatttctct tatacttact ttgtgaatac agtgaaacgc
     2821 gatccagctt atgtgaatct ttcagaagag cagatcgaat ctttgtataa tgggacatgg
     2881 atcatagcta ctgggatcgc agctttgatg tcttcaagca tcatccatcc aactgatgaa
     2941 cagattgaaa agatgatcca agacagtata gatgctatct tgaaattcaa agaaccaatc
     3001 gaaatcgatt gaatatactc aaaagttcca caaatcagta ggtaacaaga gatgtgtcaa
     3061 aataaggaaa agttcgtttt tcttacagcg tatgcatttt taactcccga agaactcttc
     3121 catgaatgca atgaataaaa gagccataca gattgaataa atctggatgg ctcttttatt
     3181 gcaagtatat taaatttttg cgactagttc tttagcgaaa gcttctaaat tttcgatatc
     3241 ttcttcttca gcattcagat ccactttaac attttcggct ccttttgatg cgcctgtttt
     3301 tgcaaaagca gcatcgaaat catccaccga tttgcagaat tcatcgtaaa aagtatcacc
     3361 agatccgcag acaccgtaaa tttttcccaa tagatccaat tcttgcaagt cttcataaaa
     3421 gtctacgatc tcatcaggca gatcgccatc atcgtatgta taagtagcta ctacgcaaat
     3481 atctgcctcc tcgaattcat ctgcatctac ttgggtacat tcgttgattt cgacttcgat
     3541 atccagattt tcaaatgctt ctgcaacaat atcggcgatt tcttcagtgt ttcctgtcat
     3601 gctagcatat acgatttttg ctaaagccat gatgtgcctc ctcgtttata attatttaac
     3661 aattatgaat tataccaaaa catcattgaa aagaaaataa aacctaaaga cacctgggga
     3721 atgtcatttg gctaaatgta tgccgctatg ttaaaatgag agggatttgt aaaaaggaga
     3781 cgaaaatatg attactttaa aatcagcacg tgaaattgaa atgatggcag aatctggagc
     3841 tttattagca gatgtacata aaaatctgcg tgattttata aaaccaggaa tcacaagttg
     3901 ggatattgag gtatttgtca gaaactatat cgagagtcat ggcggaattg ctgcgcaaat
     3961 tggctttgaa gggtatgaat atgcgacttg tatcagtata aatgatgaaa tttgtcatgg
     4021 attccctcgt aaaaagcctt tgaaaaacgg tgatttggta aaagtcgata tgtgtatcga
     4081 tttgaaaggc ggcatttctg attcttgttg ggcctatgta gttggagaat ctacacctga
     4141 gatcgaccga ttgatggaag taacgaagaa agctttgtac atcggtattg agcaagcaca
     4201 agtagggaat cggattggtg acatcggtca tgctatccaa acttatgtag aaggcgaaaa
     4261 tctagcgatc gttcgtgatt ttattggtca tggcgttggt cctacgattc atgaagaacc
     4321 agcagttcct cattacggag aagctggaaa agggttgcgg ttgaaagaag gcatggttat
     4381 cacgatcgaa cctatggtta ataccggaac ttggaaaatg aaaatggatc ctaacggctg
     4441 gactgcgtat acaagagacg gcggactttc ttgccaatat gaacatacat tggctattac
     4501 taaagatggt ccaagaattt tgacatcaca aggagaagaa ggcacttatt gatctatcca
     4561 gtcaataggt acgggagagg tacatgaaag tttggaacaa aataaaacaa aatgaaaatc
     4621 tgatgcgctt tattgagacg actcaaaagc gcatcactga ttcagaaatg agtacgacct
     4681 ctgtcgtcgt tgcttattat ttactcttgt ctttatttcc attgattatt gcttttggta
     4741 atattttacc gtttctccac atcgatcaag agactgtact gacctattta cgagaagtga
     4801 ttcctgagac cgtttaccag tttatcggtc ctgcaatcaa agaccttttg acacaaagtt
     4861 caggaggctt gttatccgtt tctgcattag cggctttatg gtctgcaagt caaagtatca
     4921 atgctttgca aattgcgatg aataaagcgt atggcgtaga aaatagaaaa aattttttga
     4981 tcgtccgttt cttctcacta attgtcattg tcttgttcat gattgctatt agtggggtaa
     5041 ctatcgttct tgggttggga cagctgattt tggatgctat tcagccaatc gtacaaatac
     5101 ccgtgaactt tatcgatcag ttccaaactt tgaagtggcc aatcacattg gtagctttat
     5161 ttgtcatcat gttcttgatt tatttaattg tccctaatgc tcagttgaag ttgaagacga
     5221 tcattccggg cacgatcttc gctactgtcg gctggatgct gttatcacaa gtgtttagta
     5281 tctatgctag gtattttgca acgaggatca gcggttatca gatcatagga agttttattg
     5341 tactcatgct ttggcttaat tttgctgcta cgatcatcat tttgggcgga atcatcaatg
     5401 cagttgttca agaatacgtg acgggtcatc aaatcaaaaa acgtcgagat ccaagccgaa
     5461 aatggttcaa taaattgaaa actaaatttt ctcataaaaa aaagtagaaa caagagttct
     5521 tattgtaaat ttatatcagt aatttataat aattatgact cttaattaaa gtaggtgccc
     5581 atatgcttgt ttcttttagt atggttataa gattttttct gacaatttta ttatcactga
     5641 tattaacacc tgtattcaaa atcatttcag tacaaacagg tatggtcgac aaaccaaatg
     5701 aaagaagaat aaataaagtt ccaatgcctt cagcgggcgg actgcctatc tttatttcat
     5761 ttgttatatc gactttattc ttattccgta aaattatccc tcaatcgtat attgtaccga
     5821 tcttgattgc ttctttagta atcatagtca caggagtgct cgatgataaa tatgagctga
     5881 cacctaaaca aaaaagtata gggatcttga ttgcttctct tattatttat tttgtagcag
     5941 atatacgtat tgattccttc acgcttcctt ttattggata tatccaacta ggctggctta
     6001 gttttcccgt aacgattttt tggatcttcg gtatcacgaa tgcggtcaat ctgattgacg
     6061 gtttggatgg gctggcggca gggatttcat tgatcggttt aattacgata ggaatcattg
     6121 gatatttttt cctccatgct tctacagtgt atattccagt tgttattttc tgtcttgtag
     6181 caagtattat cggttttttt ccttacaatt tttatccggc aaagatttat ttaggcgata
     6241 caggagctct attcctaggg tttatgatgg ccgtcctttc actacaaggg ctgaagaacg
     6301 tgacttttgt ttcatcgatt tctttgctga tcgtcatggg agtgccggta acagacacgt
     6361 tttttgctat cattaggaga aaagcaaacc gtgtttcttt ttctacagca gataaaaaac
     6421 atcttcatca ccgactaatc agtttaggct ttactcataa aggtgctgtc ctgacgattt
     6481 atgcgatggc tttgatgttt tctttcacag ctatggtcat gaattatact ggaagaatcg
     6541 gaacgatcat attgattatt gccatgttgt ttgctgcaat tttattattt gaattgattg
     6601 gtctgatcaa tgagaaacat cagtttattt taaaaacatt gcgattttta gggaataaag
     6661 agtaccgtac gcaagtaaaa cagaaatatt tgaagaaatg gaaccgtcat atagatgaat
     6721 aacaaattaa accagccgat ccttattagt attgtgacgg caaacagtga taaaattttt
     6781 cggacactag ataattttat ttcaactgta aaagatgaaa cagcagtaaa aatccgaatt
     6841 tttgacaaca attcacttcc cgagtacaaa aaaagactaa cagagtatag gaaatatccc
     6901 tacatcgata tctattttca tgatgaaaat tccggatttg gttatggaca taatcacaat
     6961 ctgatagcta gcgattctcg ctatggcatt atttgtaacc cagatatttt agtggatgaa
     7021 gagacagtta cagcattggt cttgttattg aaagaacatc cggaatgtgc gatgttagca
     7081 cctaagattc ttaatgaaga tggaacaact cagcacctca tccgtgagca gctggctgtt
     7141 tttgattacg ttttgcgctt tgtgcctttc aaatgggtca aaaaaaagtt tgacaaacga
     7201 ctggctgtgt ttgaatgccg aggattgcct actgatgcta atagttatgt acggatgatc
     7261 tcaggtagtt ttatggtagc tgatattgat aaatttaaag agataaaggg gtttgatgag
     7321 cgtttcttca tgtactttga agacaacgac ttgtgtttga gttttgaaaa agcaggttac
     7381 aagttattgt acacaccctt ttatgcagtt atccattttt acgggaaggg tgcgcatcga
     7441 aatgcaaaac tgtttcgcgt gtttttgact tctatgatga aatttttcaa taagtggggg
     7501 tggcgttttt tctgatgaaa ccagaaatat tagtagtcgt tgtgctttat cagcaactat
     7561 tcagtcaatc tccagcttat ggaccattaa gcaaagcact tagcgaaaag caaattcagc
     7621 ttattattta cgataatagt cctcaaaggc agcaaaatga gctgtttgaa aaagaaaata
     7681 tcgtttatca tcatgatccg caaaatcctg gtttagcggt ggcttataat tttgcgttgt
     7741 ctcaagcaga tgaaggaaca cgttgtctcg tcactctgga tcaggatacg aggctagtcg
     7801 acgattattt tgaactattg cgtaaagtaa cctttacaga tgaatgtgtt gctgcagttc
     7861 cgatgatttt ttctgggagc cgacaagttt ctcccctata ttcagatcgt tatatcaatc
     7921 gtacagctca agcagtagaa gttgaaggcc caacagctga acgaatcatg gcaatcaatt
     7981 caggtgttgc ctggtctgtc aaatttctaa aaaagattgg cgggtttaat cctgcttttt
     8041 ctttagattt tttggatcat tggctgtttt ggaaagtgga tcagttggaa aaaactgtgg
     8101 agatccttcc tgtacgaatg gaacatgatc tttcggtttt agactatgaa aaagtttcaa
     8161 gtaatcgata ccgttctatt ttacaagcag aaaatcgatt ttatcatgaa tatgatcaaa
     8221 ggcatttatc cagtcaccga cgacaattat tgttaagaac agctaaacag ttcttgaccg
     8281 tcaaaaaccg gcatatctgg cggatgactt tgcgctcata tgttaagaat tggaaggtgt
     8341 gaattcgtgc gttcggtttg tatagctacc tttaatggag gcagatattt aagagagcag
     8401 ttagatagta ttttatctca gctttcagca gaagatgaag tagtcatttc tgatgatggg
     8461 tcagtagatg atacatggga tatattaact gaatacgcag aaaaagataa gcgaatccag
     8521 cttttttcgg ggcctcgtca aggattgatc gctaattttg gttatgcgat ccagcagacg
     8581 aaaggagatc taatcttttt ggcagaccaa gatgatgttt ggttggagac aaaagtgaaa
     8641 aagatcactg actattttga gcagaatccg gatcagctag tagtagtttc tgatttaatc
     8701 attgtggaca gcgagctgca agagatccat gattcgtatt tttcgtttcg aaattccaaa
     8761 gaaggatggc ttaataatgt gataagaagt cactatattg gtgctggtat ggctttccgg
     8821 tcttctttaa aacccgatat tttgccgatc ccgtcagaag tgccgatgca tgatatgtgg
     8881 attggtttgc tgggaggaaa gaagactggg ttcctaagag aaccgttaac tctgtaccga
     8941 agacatgatt taaacgcgag tgagattgaa acacattcta aattacggca aaaaatttct
     9001 tggcgtgttc atctgattac ggcgttgatc aaacgaaaat ggtttaaacg ttgataaaag
     9061 atgtttaaat ccttaatatt tccatgacaa atataacaat tgatgagata ataacaaagt
     9121 gaaatttgaa atcaaaaagg agagcagata tccatgaagg gaatcattct tgctgggggc
     9181 agcggaacac gtctgtaccc tttaacaaag gcaacatcga aacaattgat gccgatttat
     9241 gacaaaccaa tgatttatta tccaatgtct actctgatgt tggcgggaat caaagaaatt
     9301 ctgattattt caacgccaca agatacacca cgtttcaagg aattatttgg aaacggaaat
     9361 gatttaggac tgcagatcga atatgcagta caagaaagtc cagatggatt ggcacaagcg
     9421 tttatcattg gagaagaatt tattggagat gatagtgtct gcttggtatt aggggacaat
     9481 atttactatg gtggcggtct gtctaaaatg ttgcaacgag cagcttcaaa agatacaggc
     9541 gcgacagtat ttggctatca tgtcaatgat ccagaacgct ttggtgtagt agaatttgat
     9601 gagaatatgc aagctttgtc gattgaagaa aagcctgctc agccaaaatc aaattacgct
     9661 gtaactggcc tttattttta tgacaatgag gtaatttcta tcgctaaagg tatcaaacct
     9721 tcagaacgtg gagaactaga gattacggat gtcaataaag catatttgga taaaggaaaa
     9781 ctatccgtag aacttatggg acgcggattt gcttggcttg atacaggaac acatgaatca
     9841 ttgcttgaag catctacctt tatcgaaaca attgaaaaac gtcagaactt aaaagtggct
     9901 tgtttggaag aaattgctta ccgtatgggc tacatcgata aggaacaatt gctggccctt
     9961 gcacagccat tgaaaaaaaa tcaatacggt caatatttat tgaatttggc cgctgaataa
    10021 ggagtcctta cagatgaaag taacagatac aaaattaaca gatgtcaaaa tcgtagaaat
    10081 ggacgtcttt ggcgatcatc gcggtttctt cacagaaagt tattcaaaag caaaatttgc
    10141 agaacatgga ttgaaatatg actttgttca agacaaccat tcactatcag ctgaagctgg
    10201 tgtccttcgg gggcttcatt tccaaaaagg agaagctgca cagacaaaat tgattcgggt
    10261 agtcactgga gcagttttag atgtagtagt ggatatccgc aaaggtagcc cgacttatgg
    10321 tcaatgggac ggatttatct tatctgaaca taatcatcga cagttgcttg tacctaaagg
    10381 atatgcccat ggattcgtta ctttgacacc aaatgtgaac ttcctttata agtgtgataa
    10441 ttactataat gcagaagcag atgggggcat cgcatttgat gatccagatt tagcgattga
    10501 ttggccaatt gatccatcaa gagcgattat gtcggaaaaa gatcaccgtc atccgacatt
    10561 aaaagaattt gaagcagaaa atccatttgt ttacggagaa atctaagaat taaggagaaa
    10621 ttttcttatg aaaaacatca ttgttaccgg aggtgccgga ttcatcggct ctaatttcgt
    10681 tcattacgtg gtaaacaatc atccagaagt gcatgtcact gttttggata aactgactta
    10741 tgcaggaaac aaagagaact tggccggact tcctagcgat cgcgtagaat tggtcgtagg
    10801 agatgttgca gatgcagaat tagtggaccg tctagtaaaa gaagcggacg cagttgttca
    10861 ttacgcggca gaatcccata acgataattc attaaaagat cctttcccat ttgtacaaac
    10921 gaatatcatt ggtacgtata cacttttaga agcttgccgc aagtatgatg tacgctatca
    10981 tcacgtatct actgacgagg tttacggcga tcttccatta cgtgaagacc tgccaggaca
    11041 tggggaaggc gaaggagaaa aattcactgc ggaaacacca tataatcctt caagtccata
    11101 ttcttctaca aaagctggtt cagatctgtt agtaaaagca tgggtgcgtt cttttggctt
    11161 acgtgcaacc atttcaaact gttcaaacaa ctatggtcct tatcagcata ttgaaaaatt
    11221 tattcctcgc caagtaacga gtatcttaag cggtattcgt ccaaaattat atggcgaagg
    11281 aaagaatgtt cgtgactgga tccatacgaa tgatcattct tcagcagtgt ggctgatttt
    11341 gacgaaaggc agaattggcg aaacctatct gatcggtgca gacggtgaag aagataataa
    11401 gaccgtgatg gaattgatct tagaaatgat gggccaacca aaagacgcat acgaccatgt
    11461 aaacgaccgc gcagggcacg atttgcgtta tgcaatcgat tcgacaaaat tgcgagaaga
    11521 gctaggttgg cagcctgagt ttacgaactt ccgtgatggg ttggctgaca cgatcaaatg
    11581 gtacgaagaa catgaagact ggtggaaaaa agaaaaagag gccgttgaag cagcatatgc
    11641 caaaaatgga caataataca gaggaaattt cactgtatta atggaaaaac acatgtgagg
    11701 caggcgctcg cctgtctctt ttttaaaata aaaatcaagg agcggataac catatgtttt
    11761 tattaactgg aggaaatgga caattaggta cagaattgcg ccatttatta gatgaaaaaa
    11821 atctgccata tgtttcaaca gatgcaaaag acttggatat cacagatgct gagaaaacaa
    11881 tggcttatat tacagaatta aaacccgatg ttattttcca ttgcgcagct tacacagcag
    11941 tagataaagc agaagaagaa gcaaaagaat tagatgagaa aatcaatgtc gatggcacaa
    12001 gaaatgtagc acttgcagca aaagctgtga atgcacaatt cgtctatatc agtacagact
    12061 atgttttcga tggaaaaaac aaaaatgtag aatacgaagt agacgaccaa acgaatccac
    12121 taaatgaata tggtcgtacg aaattattag gcgaaaaagc agtagaagag attttagaag
    12181 actattatat catacgaact tcttgggttt tcgggatcta tggacacaat ttcgtcttca
    12241 cgatgcaacg cctagcggaa acccgcgaca aactgactgt agtaaatgac cagtttgggc
    12301 gaccaacttg gacacgtact ttggcagaat ttatggtata tgtcatcgaa gaaaaagcac
    12361 catttggtat ttatcatttg tcgaacgaaa atagctgttc ttggtatgag ttcgcaaaag
    12421 aaatattgaa agatacagat gtagaagttg cacctgttac gtcagaagaa tttccgcaaa
    12481 aagcaacacg tccgcagtat tcagtcatga gcctgaaaaa gacagaagcg ttaggttttg
    12541 tcataccaac ttggcaagaa gcacttgcgc aaatgctgga agctgccaaa aaaatgaata
    12601 agtaatttcc tttttatcaa atagaatttg gagcatgata tggaaaaaca aacaagaagt
    12661 gcacgacgaa aagaaaagcc aaaagcgaat caggcagatt cgaatttggt gcttgacctg
    12721 tttctaaaac tccgctatct tattggcgtt ttagtcatcg tggtcatcgt tacttttaat
    12781 ttaaacggaa gttcacttgg tgtttgggat aaatatgtgt cgcaaagaga cgatgggaaa
    12841 aaaactgatg tgatttttgg acagaatcgg gaagtgcggt cagacgaatg gctggtgcag
    12901 acaccttttt acctctcaca agctgaagag gacttttcgt tggtgaacga ggattattca
    12961 ttaaacggac aaaacatgat cattgcgtat aattctcctg taaaggatat tacggttatc
    13021 ggaaaaccat tcaactgggg attctttttc ctcggaagag atcgaggact ttccttttat
    13081 tgggcgatga aattagtcgt catggtcttg cttggattcg aagtactgat gatattaacg
    13141 aagaggaaaa aagctctatc gctaatcggt gcatttgcgc tgacattttc tccagcagtc
    13201 cagtggtggt tcatgcaaca tgtgggcgat ttgattttct ttactttggg actgatgatt
    13261 gctttttatc attatttcta caatcatgat aaaaaatggc tacgaacgtt gatgatgctt
    13321 ttggtggtta ttttcggtat tgggtttatt ttagtgattt acccagccca tcaagtgatg
    13381 ctggcgtact tgctgatttt ttattttgtt ggcctgctta tttactatag caagaaagtt
    13441 tcatgggatc tgttggatgt agtgttgatt ggaggggcag ttttatttat tggcggagtc
    13501 atggttcatt tctggctaac gtcaaaagac gcattgttag cttctttgaa taccttatat
    13561 ccaggaaaac gggtaagtac tggaggaaag tggacgattg gcgaattttt ttctttcctt
    13621 acaaattgga aaattccttt tgaagatatc aactattcga ataactctga agtggccctg
    13681 ttctatcact tttttccaac tgtattccta gcaagtccat ttgtgttatt tggcaaaaaa
    13741 gatagcgaac aaaagttact gggacgtatt ttgatgattt tttgtctgtt ttctatcttt
    13801 tggattaccg ttgggttgcc aaaatggcta gcagaaatca ctctgctgtc ttatgttcct
    13861 ccattaagag ccatccttac ttttagtttt gcggcttgtt tattatcgat ctggtttatc
    13921 gcttatattt ggagagaaga tgttattcct atttggtata aattcatttt gttggcagta
    13981 aatgcatggt cttatttcta tgcgattacg cattcgaaaa tggataatta tttttcggat
    14041 tttgaaacca tcgcagttgc tgtgttggga acagtgattt tagcttttgc attatttggc
    14101 tggagacgct tgttttacct cgcttttgtc gccttagtta tcgtatctgg ctttttcgtc
    14161 aatccagtcg tagaaggtac aggagcgatt tttgaaaaaa cactggcaaa agaaatccaa
    14221 aaaatcgatc aacaagatcc tggtaaagtt tggttgtctg aagatgaatt atataacttc
    14281 accccaacat tgggagtgaa atccttcaat acagtacgct tttatccaga tatagctgca
    14341 tggaagaaaa tcgatccgaa agaagaatac gagaagtatt ataatcggta tgctcataca
    14401 agagctttta tcgctaagga agatacaaag ttcattttag atcgtccaga tatgttttcg
    14461 gtcacgttga attttgaaga cgcagaaaaa ttagggatcg actatgtgat cagtaaacga
    14521 ccattaactg attacaacca attatatgaa gcgcagttca cgcagctata tggaccggat
    14581 aaagacggtt atatgatttt taaagtagaa tatccggatt atccagatgt accacaaaca
    14641 caaactcagg aaaatcagct agtcgtacag taataggcta gctgagaata atcctgaaat
    14701 attagtagaa aggagagtga agataattca agcaatcgta caaaaatttg tcaacaaaat
    14761 gagtgtcggt ttcttgcttt tgttgctgac gctagtcttt tttgtttgga cagcaggggt
    14821 aggttttcca gcaactttgt ttatatataa caatagttca ttacttatcg cttttagtgt
    14881 ggctattttg ctcattttaa atgtttataa agcaaaaggc gtcgattttc tttatctagg
    14941 tgttgcgctt gcccttttca ttttcttttc ttactttcgg gaattaagaa atgagtcttt
    15001 atttggagac ttactgtttc ctttgattat tttattttgt ttagtaatta aatggatcga
    15061 gtggacaaaa tgggatcgcg gtatttatct gattgtcttt acggtcttcc tcggaatcac
    15121 gttatatcgt gtatttacag agatccaagt accagaagga caaagcatct gggctccagg
    15181 caattcgctt tctgatattt ggatcaatac gaatacaatc ggaagttctt tgatgactct
    15241 tgctttattg atcagtggat ttgccagttc ttttgaacga tggtatgttc gaatcatcgg
    15301 tgggatagca gtcgttgcag cctttttagg tatttgggtc tgccaatcta gaggtgcatt
    15361 agtcgcgttg attgtttttg cgatattgga tcttttacct aaatcgttga tgcgtgtcat
    15421 tcgtgcgcca tttattgcct acacggttac gattctttta gctttgccga tttcttatct
    15481 ggcggctgtc tctgaaaaag tcaatttgtt taccggtcga gaagatattt ggcataaatt
    15541 ctatcagacg ctaggagaaa aatctgaaca agttttacta ggaatgaaaa catttatctt
    15601 ccagcgagga aaccaatttt taggaaacca caatagttat aattctattt tgaatatcta
    15661 tggattgata ggatttggga ttgctgcttt gctgcttatc ctttttattg gtcgcttaac
    15721 gttgaaagca gatttatcca acgggcagat gacttttatc tgggcctttt ttgcggtgat
    15781 gatgcagtca tttatggagg atacattgac ttcataccca tgggttccga ttatttatat
    15841 cctgttggct atggccgttc atcgatatga tgaaccagaa gttacggagg aattctatgt
    15901 tatggaagag gcatatgacg atatgactta tgatgataca tcttatgagg aacaaccaga
    15961 tagcgctcat tcttccagga tccaacgcta tcattgatag gcacaaaata cgagagaggc
    16021 tgtgacaacc gtcgcagcct tttttggttt attgcaattg tctagcatct tctgtaaaat
    16081 agaaacgtta gagaaaatct tgtatcatag aaaattaaga aagaaataag aacgcagctc
    16141 ggtaccaaaa atatggtaaa atggcacttg actgtaattc actgaaaatc gcttggagga
    16201 acacaaatgt ttaaagattt gtatttattt atgaaggaca tttatcagaa cagaaaatta
    16261 ctgctacagt tttcgatcaa tgacttcaaa tcaagatatg caggatcgtt tttaggaatc
    16321 ttatgggcgt ttatcaatcc gctatttact gtattgattt attggctggt atttggtgtt
    16381 ggactgaaag cacaaatgat cgatgcaaaa tacccattta tcgtatattt agttaccggg
    16441 atgattccct ggttcttctt ctcagatgca tttgcatcaa ctacggttgt ttttcgggaa
    16501 tatacttatt tagtaaagaa agtcgtattc aatattcgta tcttgcccac gacgaaaatc
    16561 ctatccaatc tgtacaccca tttattcttc attctgattg gcatagctgt cgcgcttata
    16621 aatggtatat atccttcagc aatgtcttta cagctgattt attatttatt ttgcatgtgt
    16681 gctttcttga caggattgac ttggctgacg gcatcgatcc aaccattttt acctgatatc
    16741 atgcaattaa ttacgatcgt gctgcagttg atcatgtgga cattaccgat attatggagc
    16801 cctaatcagt ttgattcagg tcttgtaagc atattgaaat taaatccgct gtactatgtt
    16861 gttcaaggtt atcgtgagtc atttttaagt gaagcttggt tctgggagca tggaacatat
    16921 actttgtatt tctgggtttt cacgttagta atgttcgtaa tcggctctgc agtgttccgt
    16981 agattgaaac cacatttttc agatgtgctg taaggagaat agctatgaca gaatatgcaa
    17041 ttagactaaa agatatcaca aaatcttata atatgtacgc aaaaccttcg gatcgatttc
    17101 gtgaagccct caatcctttt aagaaatcct atcatgatgt attttatgcg ttgaaaaacg
    17161 tgaatgtaga aataaaaaaa ggtgagatga tcggtttcgt gggagaaaac ggatcgggaa
    17221 aatctacaat gttgaaaatc attacgggag tactgacacc aacatccggc acgatggaaa
    17281 tcgaaggaaa aatctcagct ttgctagaac taggatcagg attcaatcct gagtattctg
    17341 gctatgaaaa tatctattta aatggaatgg ttctaggatt ttcaagagaa gaagtcgatg
    17401 aaatggtcga tgatatcatt gaatttgctg acatcggcga ccatatttat caacctgtaa
    17461 aaacctattc aagtgggatg ttcgttcgtc tagcttttgc tgtagcgatc aatgtgaatc
    17521 ctgatatttt gattgtggat gaagcattgg cagtaggaga tcttgaattt caactaaaat
    17581 gtatggaaaa atttacagaa atcaagaact cgggaaagac gattttattc gtttctcatg
    17641 atattagttc gattcgccgt ttttgtgatc gttcctattg gttgaaaaat ggaaaagttg
    17701 tcgaatatgg tgatacgatg gaagtaacga cgaattacga aaactttttg aagaaaaaat
    17761 cagttaaaac agtagatcga aataaaaacc aagtagaacg cttcgcagct ccagatatcg
    17821 tagacgtgac gaaagccgaa ttgttggata aagataaaaa tccagtggat atgctggaac
    17881 aaggagaaaa actatacgtt aaagtaacct atgaagtaaa agacgataca atcaaaaatc
    17941 cagttttagg aattgctctg cgtacagtcg acaattttta tgtgtgcgga ctcaatactc
    18001 tactagatgg tatcaagatc ccttggaaaa aaggtgagaa tatcttttat ctggagtatg
    18061 aaaagttagg tttgttagct ggtgaatatt attttgacgt tgcggtcttt gaagaaaatg
    18121 caacggttcc tttggtatac aagacaaaat acatgaactt gtttgtcagc ggttcttata
    18181 tcggagaagg catcgtggtc ttagaccata aatgggaaga agggactcat agcaatgaaa
    18241 tatgattttg aaatggacct agatgaacaa agcagtgtgg gaaaaattgc cgcacagatc
    18301 aaaccaggaa gtaaagtttt agagtttggt cctggaaacg gccgattaac gaaacatttg
    18361 atcggagcaa aacaatgtga agtgagcatt gtcgagctag ataaggaatt gttcgatttt
    18421 gtcagcgagt ttgcccaaga tggattttat ggagatatcg aaagttttga atgggcaaac
    18481 tattatgctg gacaaacatt tgattacgtt ttgtttgccg atgttttaga acatttggta
    18541 gatcctggaa aaacgttgaa gaaagttaga gaatttttga atgaagaagg agaaattcta
    18601 attacattcc ctaaccttgc gcataattct gtcatgattg atttgttcaa taatcaattg
    18661 ccatgggctt cttatggttt gctagatgaa acccataatt ctttttatac tcatgatggc
    18721 ttccaaaaag tttttgaaaa agcaggatta tacatcaata ttgaagatta tttgtactta
    18781 gctgtaggtg atacagaatt aaaatcaact tatgaagaat tgccggaagc tgtacgttat
    18841 gatttcaaaa tgcgaccatt tggcgaagtc taccaatact tcttctcatt gatgaaacat
    18901 ccagtagcac aaagttccat tgcgcagccg caaaattcta attatgtgaa agtattagaa
    18961 gtaatccagc aaacgaaaca agatgaaata attcaacaaa taccattcaa taatttcaca
    19021 ggggaaaatg agacactaag tttccatgta tcagaattag cagaacgaat ggtgtttcgc
    19081 ttcgcgcagc aacctagctt tatcgaattc agcgcagaag ctgctggaga aaaactcaca
    19141 tttatcgatt caaatgctgt agtcaaaaca gtaaatgatt gttatttgtt tgatggtaaa
    19201 gatttaccag agttcttttt aacggatatt gcagggaaag aagtgactat ccattgtcac
    19261 tatcgtttta tcggagaatt gacaccaaca atgaaagaat tgctggaaac aatccgtcca
    19321 atggctgaag ttacaaagca gctttctgag aaaaatgatg aattagaaag agaaaatcat
    19381 tatcttattg agaataatac aagattagat cagacgttga aaacaaccat aaatcgctat
    19441 tgcacgttgt tggaaagtga tgaatggaca atcaaacata gattagggcg acgtaagaaa
    19501 gaaacatcga aaaaaatcca agaaaaagag ctttctctat gtattgacga aaaaatctgg
    19561 gatgcagaaa caaagatatt gaaaatcatc gggtggggaa tcgctaattc ggatcgccaa
    19621 ccattgtcct ataagttatc agccgaccaa tcaccatttt ttgaagcaat tcctgtttta
    19681 cgagaagaag tcaatcaagc aaaacagcta gctgaaggag caaaagcagg atttgaactg
    19741 cggatccttt gcgagaagga acgttcattc ttagttgaag cagtggctca aaatggtcaa
    19801 tcatggatca ttgaaatgta aataatgatt aacttataaa gataaggagt aaacgaatcg
    19861 tgcaaaaaaa agaaattgcc gtatttatcg atcaaatcac tcgtgaacgg gccactggcg
    19921 atttgcttat tgtgggctgg gcaattgatg aagtgaccaa agaaatccct acgataaagg
    19981 tagaaaaaga aaatgtgatt gctgaagcaa cacatgtcgt aagattggat atcaatcatt
    20041 tatataatct tgatgttaag acccaaagcg gttttaaggt acgtttatca gggaaaatga
    20101 gaggaaaagc aatcttagac ttccaaacag caagacatca aaatggcata gctgtgaaat
    20161 taaacggtaa atatccttat gatgacggca ttgaatcttc atgggaaaga aaaaaaagat
    20221 tactgaaaaa agggataaat tatgcaagga ctcatggcgt aaaaaaagca atccgccggg
    20281 tcaaactgga attgaaacca ggatcgatcg actatgcaga gtggatcagt cgtcatgaga
    20341 aacctgatct gaaagagcaa aggaaagaag tacaaggatt tgcctaccgc ccattgattt
    20401 ccatacttat gcctgtctat aacgttgaaa tcaaatggct cgaaaaatgc attgattcgg
    20461 tacttgatca aacgtatgat cactgggaac tatgtatcag tgatgatgct tctactgatc
    20521 cagctatccg aaagtgtcta gaatcctatc aagcaaaaga tgatagaatt aaagtcgttt
    20581 tccgacaaga aaatggacac atcagtctgg caactaattc tgcattggaa atggcagaag
    20641 gagagtttat agcgctctta gataatgatg acgaattacc tccatttgcc ttatatgaag
    20701 tggcaaaagt gctaaacgta catccagaac tggatttgat ctacagtgac gaagataaaa
    20761 tcgatgcaga tgggaatcgc tttgacccgc acttcaaagc agactggtct cccgatacat
    20821 tgatgggaaa taattatatc tctcacttag gcgtatatcg gacaagcatt gttaaggaac
    20881 ttggcggttt ccgtaaagga tatgaaggct ctcaagatta tgatttggtt cttcgtgtga
    20941 cagaacaaat tcctgcagaa cacatttacc acattgaccg tgtcttgtat cattggcgga
    21001 cgatcccggg atcaactgca agcaatggag aagctaaaag ctacatctat gattctggag
    21061 tgaaagcatt gacagatgct ctatctagaa gaaatatcaa gggaagcgta catcctggca
    21121 gaatttctgg tttttatgaa atcacttatg atgttttgca agaagacttg gtcagtgtga
    21181 tcattccaac taagaatggc tatgaagatc taaaaacttg tgtggattcg atcatcgaaa
    21241 aaacgtctta tccaaattat gaaatcatta ttgcagataa tggaagtaca gatcctaaaa
    21301 tgcaagaatt atttgcagaa tacaagcatc agctgaaaga ccgatttatc gtcgaattaa
    21361 ttgacattcc attcaattat tcacgtatca acaatctggc agcagaaaaa gcaaacggca
    21421 aatacttttt attcttgaat aacgatacag aagtgattga acctgactgg atgacagcga
    21481 tggtttctta tgcacagttt gatcgaatcg gctgtgtagg agcaaaatta ttttatccgg
    21541 atgatacaac gcagcatgca ggtgtattat tagggatcgg tggcgttgca ggacatgcat
    21601 tgaataacta tgatcgaaca cattgtggtt actttggccg attggtcatt gacgtcaatt
    21661 acttagcagt cactgcagct tgtatgatgg tcaaggcagc tgacttcaat gctgtcaatg
    21721 gatttgatga aatattagaa gtagcattca acgatgttga cttgtgcttg aaagtttatg
    21781 aactgggtcg ttacaatgtc tatgcacatc aagcagaatt gtaccatttc gaatctaaat
    21841 caagaggtta tgaagataca ccagaaaaac aaagacgttt tgctggggaa atcaaaaaaa
    21901 tgcaagataa gtggccttct tatattgctc atgacccatt ttataacgat aatctaacta
    21961 aagaaggaat cggcgatttt tcgttgagac ctgattgatt aaaagaactg tattttcatt
    22021 tttattaatg aaaatacagt tcttttaatt taatt
//
LOCUS       DACEPD010000047        21925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.048, whole genome shotgun sequence.
ACCESSION   DACEPD010000047 DACEPD010000000
VERSION     DACEPD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.048"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            284..523
                     /locus_tag="IW797_001641"
     CDS             284..523
                     /locus_tag="IW797_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224424.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(885..1145)
                     /locus_tag="IW797_001642"
     CDS             complement(885..1145)
                     /locus_tag="IW797_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224425.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1330..1827)
                     /locus_tag="IW797_001643"
     CDS             complement(1330..1827)
                     /locus_tag="IW797_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ1224426.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1957..2667)
                     /gene="budA"
                     /locus_tag="IW797_001644"
     CDS             complement(1957..2667)
                     /gene="budA"
                     /locus_tag="IW797_001644"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ1224427.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2680..4344)
                     /gene="alsS"
                     /locus_tag="IW797_001645"
     CDS             complement(2680..4344)
                     /gene="alsS"
                     /locus_tag="IW797_001645"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ1224428.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4549..5211)
                     /locus_tag="IW797_001646"
     CDS             complement(4549..5211)
                     /locus_tag="IW797_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1224429.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5221..6036)
                     /locus_tag="IW797_001647"
     CDS             complement(5221..6036)
                     /locus_tag="IW797_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1224430.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6298..6741)
                     /locus_tag="IW797_001648"
     CDS             complement(6298..6741)
                     /locus_tag="IW797_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ1224431.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6875..7213)
                     /locus_tag="IW797_001649"
     CDS             complement(6875..7213)
                     /locus_tag="IW797_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1224432.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7201..7578)
                     /locus_tag="IW797_001650"
     CDS             complement(7201..7578)
                     /locus_tag="IW797_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1224433.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7802..8995
                     /locus_tag="IW797_001651"
     CDS             7802..8995
                     /locus_tag="IW797_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1224434.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9161..9583)
                     /locus_tag="IW797_001652"
     CDS             complement(9161..9583)
                     /locus_tag="IW797_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1224435.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9978..10172
                     /locus_tag="IW797_001653"
     CDS             9978..10172
                     /locus_tag="IW797_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224436.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            10169..10663
                     /locus_tag="IW797_001654"
     CDS             10169..10663
                     /locus_tag="IW797_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224437.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(10825..11997)
                     /locus_tag="IW797_001655"
     CDS             complement(10825..11997)
                     /locus_tag="IW797_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1224438.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12203..13018)
                     /locus_tag="IW797_001656"
     CDS             complement(12203..13018)
                     /locus_tag="IW797_001656"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1224439.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(13168..13614)
                     /locus_tag="IW797_001657"
     CDS             complement(13168..13614)
                     /locus_tag="IW797_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1224440.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(13611..14615)
                     /locus_tag="IW797_001658"
     CDS             complement(13611..14615)
                     /locus_tag="IW797_001658"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ1224441.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(14655..14984)
                     /locus_tag="IW797_001659"
     CDS             complement(14655..14984)
                     /locus_tag="IW797_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ1224442.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(15110..17437)
                     /locus_tag="IW797_001660"
     CDS             complement(15110..17437)
                     /locus_tag="IW797_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1224443.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(18280..18573)
                     /locus_tag="IW797_001661"
     CDS             complement(18280..18573)
                     /locus_tag="IW797_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ1224444.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(18976..20154)
                     /locus_tag="IW797_001662"
     CDS             complement(18976..20154)
                     /locus_tag="IW797_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ1224445.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(20258..20572)
                     /locus_tag="IW797_001663"
     CDS             complement(20258..20572)
                     /locus_tag="IW797_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1224446.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            20679..20894
                     /locus_tag="IW797_001664"
     CDS             20679..20894
                     /locus_tag="IW797_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224447.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(21194..21436)
                     /locus_tag="IW797_001665"
     CDS             complement(21194..21436)
                     /locus_tag="IW797_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1224448.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(21423..21851)
                     /locus_tag="IW797_001666"
     CDS             complement(21423..21851)
                     /locus_tag="IW797_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1224449.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         6454 a         4314 c         3707 g         7450 t
ORIGIN      
        1 gctatcctcc tagcaactat ttaaataccc aaaaaaatta cctctagata atatcatacc
       61 tacgttttca cttataaatt ttttcataaa aaaagcgttt agaggcataa tcgttaatac
      121 agatggtaac caatgtatct ggtttgaact gggatatcac cctctccata tatgtattac
      181 taagactaat agatactata aaagacttga tagttttgca ttgattttgc ctaatactga
      241 gcataggtgt atgctaaacg atactataag gagtgtgtac ataatgaaaa gaattctgtt
      301 tatcagttta tcaaatttat tgttacttac tgcttgtggg acttctgcat caaagaacat
      361 accttcagat acgcaaattt cgaatagtat cgattcaaca atttctacaa atgagaaaaa
      421 ttctccggta gaaacttccg atataaaagt ttctacctct gcaaaaaaca gggtaaagaa
      481 actgtcagca cgtctccttt atttcaaaca aactctgaat tagcttcttc atcttcttca
      541 gcaaataatg atgatagttt cgcgtttact cctaactaaa tgcaaccttt acaaatgctt
      601 tacgataaca agcctgagct aaatgatcct gatatcagtt ttgcattttt tgaaatgatt
      661 ggatcggatg atctttttaa aactactagt ttgtctatcc gaaaatagga cagttctggc
      721 agtataggtt tttatcgagt ttctccacca ggcctcgtta cagagacaga ttctctaggc
      781 gaaccttttg aataaatcaa aaatcatgaa caatcactgc caactaaata aagtgccttc
      841 aaaagcttgg tcttctcgct tttgaaggca ctttatttaa atctttatga aactaaagta
      901 tgttcatatt tgttgctata gttatataga tattctgttg tttccaactc ataaacgtga
      961 gtatattttg ccactacttt tccgcatttt tctaatattt cattgcgatc gacgtcatga
     1021 tattcgtcta cgcagatcac aacacaattt tctaatttac tgattacttc tccaaccact
     1081 ttttgtttca agccaatagg ttggcaaata tatttttttc ctaaaatgat agtttcgtta
     1141 ttcatatttc atccatcctt aatgtttttc cattatttaa aatgataata ccattaaaat
     1201 atgattatga aaatcaataa cacttgtgat tttataaaaa aataaaaaaa ccgaaaaaca
     1261 gaaatgccca ctgtctttcc ggctcttttt attcttgatt tctcttcttt tttgtttcaa
     1321 tagacgattt tagttattaa taaatgaaaa agcatttata ttaaaatttg ctaataaaag
     1381 atctacatat ttttctagaa atgcttgatc gatttcatca taccctttcg ttactccgca
     1441 atcgatatct aaaacaccga ttaaattacc ttcttttacc attggcacta cgatttctga
     1501 ttgtgcggct gaatcgcagg aaatgtaatt tgcatgggtt ttgacatcat caacgatcaa
     1561 tgtttcttgt ttttcagctg attctccaca aactcctttt cccatcttga tacgtgtgca
     1621 agaaactcgc ccttggaacg gtcctaaaat caattcatta ccatcgaata aataatatcc
     1681 tgcaaagaca gtctcaggca aagtctcatt caacaatgca gatgaatttg ctaaatttgc
     1741 taaccagtta tcttctgctt ctaataatcc ttgttgctgt aataataaaa tttcgtatgc
     1801 atcttttttc tgtttttctg tccacatgat aaaaaccgct cctttttgat ctatggagaa
     1861 gattatagct cagttttttt gtaaacgcta ggtttcctcc tcaaaaaaac gagatacgtc
     1921 aggttaatca ccttgaacgc atctcgctgt ctttttctat tctgcttgtt ggatttctgc
     1981 tgctaaatca gcataatcaa tcgtttcgtg aacaaaagtc ggatcatcaa ctggaaaatg
     2041 ctgtacaagg gacgagatgc ggctaatctc aacttttcct tcagccactt caaaatccat
     2101 tacatgtccg ccaaatgtat gtgtatcatc gataaaatgc aagtgaaatc ccgctgcagc
     2161 aactccttgg aataattcag gcgtaaagaa cccgactaat gttccttgga cattgctttg
     2221 ttggaattct ggttgagact ctgaaatttt tgctagtctt ctgtaaggtt tttcttgctt
     2281 cggcatgata cggatgtgca tatttttgaa taatccagtt atcttcactg ccaaaaatag
     2341 gttttccccg gcttctctta ctaatatgtc ttttttcaat tgttctccat ctgcagtttt
     2401 tgagacagaa aaactagtat ctggcgtaaa atctgtaatt gctgcatagg gggtaagttc
     2461 acttccatct aattgggcaa ctgagccatc tgatcttcct tgataagcta cgccatctaa
     2521 aaagatgact tcaccatcaa gcccatgcaa tgttcccaat cccagagttc ctttttctaa
     2581 tatatgagca atcgtttctg ttccatccat caagccagcc atcaaagctc ctaatgtccc
     2641 gtgctggtat aagatttttt cagtcatgtc gacacttcct taatagaatt gatctggtaa
     2701 gattgtttct cccaatttct cattatcacg gtaatcaatc ggaatatcaa taattacggg
     2761 tccttctgtc gcaaatcctt cttctaaagc tttttctaat tcctctacac ttgtagctcg
     2821 gattccttta gcgccaaaag cttctgcata tttcacaaaa tcaactggac caaagtctac
     2881 acctgacgcc cgctgatatt tcattttttc ttggaactct accatattgt agtgtccatc
     2941 attccaaatc aaatgaacga tattcaattt tttacgtacc gccgtttcta aatcctgggc
     3001 agagaataaa aatcccccat cgccggaaac tgatacgatt tgtgtatttg gacgcaccaa
     3061 tgcagcagaa atcgcccatg gcaatgcaac accaagcgtt tgcataccat tactgaataa
     3121 caaatgacga ggttcataac ttctaaagtg gcgtgccatc caaatataat gactacctac
     3181 atctacggta acagtcatat catctgttac ttttgactga agcgtgttaa taacttctaa
     3241 aggatgcaag atacctgctt ctccttttga ttgaacaatg tcccgctccg ttaattctgc
     3301 ttgaagagat gctaaatatt cttttgcatc ttctgacact tgttgaggct ctaatgaacc
     3361 tgtcaataaa tcaagagtgg ctgagatatc accgatcaat tctctttcag gctgcataaa
     3421 aggatcgatt tcagctggcg cttcatcgat cacaatgatc cgcgcatctt tttccgcatt
     3481 ccagtttctt gcttcatatt cgattggatc atagccaata gcaataacta gatcacttcg
     3541 tttcaaaagc atatctcctg gttggtttct aaataaaccc acacgaccaa agaaatgcgc
     3601 ttctaattca cgtgagatca ctccagctgc ttggaatgtc tctacaacag gcaatgctgt
     3661 ctttccaact aattgcctga tggctaacgt ttctttttca cttgatcctc gcatacctac
     3721 taatagtacc ggtagttttg cttctctaat gcgttcagct aggtaacgga tatcatctgc
     3781 agaagcagaa cctaatttag gatcgctcaa tggcttgatg acattccctt ggacaggtgc
     3841 atccactaca tcttgcggga tactgataaa gctcgcgcct ttttttgaac tcttagccat
     3901 acgataagca ttggcaatca cttctgaaat tgtttctgga tcttggattt ctgcactgta
     3961 ttttgtgatt ggctggaaca gtgctgcatt atccatgctt tgatgggtca acttcaatag
     4021 atcgcttcgt tttacttgtc cggcaattgc taaaactggg tcaccttctg ctgtcgctgt
     4081 taccaaacct gtagctaagt tactagcacc aggaccactt gttgcgataa ctactcctgg
     4141 ctctcctgta atacgtccga ttgcttgtgc cataaatgca gcattttgtt cgtgacgagt
     4201 cacgattaat tcaggacctt gatcctctaa ttcattaaat acaccatcga tttttgcacc
     4261 tggtataccg aatacataat caacatgatg attaatcaaa ctttctacta cagatttcga
     4321 accttgtttc attttttcgt ccattgaaca tcgtcctttt tatgctttat ttttaattat
     4381 tagttagaaa taatcacaaa taattgatac agtttctaaa cctgtttcat tcaaaagtat
     4441 agcacgtttt atttcatttg tttagccata tgtttcaaaa aatgaaaaag aatgacagtt
     4501 tttacataaa aactgtcatt cttaattgaa agtttcaatt tataaagtct acagatccat
     4561 agttctcatc aatttacgat tgttgaagta aatcaaacca aatacgatag aaggacctag
     4621 tagaatacac cattggggct ttacgaaaaa gaagagaaac aaacttgtca atagtaaaaa
     4681 atctagtaaa ctcacatacc attttttcaa cgtatagtag aacgctatcc gtagaacttg
     4741 atttggtttc gacgctggat ttctcacctg aaaataccag tagttgatgc taaaggaaac
     4801 tgataaacaa gcaagtaaaa caaacaacgg aatcaaccat tttccataag aaaaacgtat
     4861 aaaaaagaaa caatcaaaaa tcattatcac actaactatc caagcaaata gccaataaaa
     4921 aaatcccttt agccaaaatt ggcgaaacgt ctttatgaac aaacgtactg gtgaaaaact
     4981 tttttctttt gctaattctt ctgtcatacc tatcatcacg atgcaacctg gaccaataaa
     5041 aagtaaagaa aaaataaagg aaaagacatt ccttccatct atcgacaaaa ggaacgcagc
     5101 tgctatatac aagatattga cacaccaaaa acaaaggcta cttgccaatg attgatacaa
     5161 ccaataaaag aattccatat aaacattttt gtcaaaacgc tgctctttca taatcacctc
     5221 ctattccttc attcctgatg aagcgatccc ttctacaaaa tatttttgca tcgctaaaaa
     5281 tataaaagca acaggaagaa cagaaacaac tgaggcagcc atgattaatg cgtactctgc
     5341 atcaaatagc ccaataaaca ttttcaaccc taactggatt gttttatttt ccgtagaaga
     5401 caaataaatg aatggcccca tataatcgtt ccatgtatta acgaatgtga tgatcgtcaa
     5461 actggcaatc gccggttttg taagaggtaa aatgatcttg cgatagattc cccactctga
     5521 taagccatcg atccgcgcac tctcacataa agaatctggg atactactgt agtattgctt
     5581 caacaagaac acaccaaaag cattgaacgc ttgtaatagc accaacgacc acaacgtata
     5641 tgttaaaccc acttttctca tcataatgaa ctgcgggatc atataagact gccaagggac
     5701 agcaatcgtg ccgatatatg ctaaaaataa aatatcgcgc cctttaaaat ttagtttaga
     5761 aaacccgtaa gcagcaaaac tagaagtaaa caactgaatc aacgtgatca caacgcttaa
     5821 aaaagctgta ttcttaaaaa atgtcactaa aggaatacga gaccaaataa ccagataatt
     5881 ctctaaatgc caagttttag ggatccattg aatcggaatc gtgaacactt cattatttgc
     5941 tttgaatgaa gcagaaacca tccataaaaa aggaatgatc gtcaataaag ctaatacaat
     6001 cagcagaatc atacccaacc attgaataaa gggcatgttt tttgaacgaa tttttgcttt
     6061 ttttctttca tttcgaacac ctccaaagtt ataatcttca tttctttgta atgattacaa
     6121 tccagaacaa cggttattat agctgattca tttccgttct tcttatgttt gtaagcgctt
     6181 atttaacaag ttatgtaaat ctctttattt tttttgattt aaaaatgaaa aaatacaaaa
     6241 gcaagactgt catacgtctc gcttttgtat tgaaatttct tcgattagag atacttatta
     6301 ctgttcaaaa gcttctttga aacggtgaat caattcattg actacaggtg ataaaatccg
     6361 attctttttc cagactagca tgcagttcgt ttcaatcatt gggtcaagcg gaacgaatgt
     6421 cactcgcttt gaaagatgac ttacagcacc ttctatgact aaagcagcac ctacctgatt
     6481 ctcaattagc gcaaatacat tgaaaatcaa attgtacgtt cctgtgatca gcaactgctc
     6541 cttttttatg cctaaccagt cagcaaacat tcgctgtatt tctggacgct ttgagatcaa
     6601 aagaggtaca ttcgcaaagt cttcttttgt tatcacgtct ttttttgcta aaacagaatt
     6661 ttctaaaatg agcaatcccc atttttcctt tcttggtaac aaaatcgttt cgtatttctc
     6721 tgtatctact ggatctagca aaatcgctaa atctaacagg cttttttcta gtcactccgt
     6781 gatatcatca cttgttccgc tgtggatatg aaattgaacg ctgggataat cacggatcaa
     6841 cttttctagt aatagtgcca atgtattcga attatcagct tccacacagc cgatcgtaaa
     6901 tcggccgctg aataattgtt tttttctgtt ctcgaattct tgagtggttt gattcgttaa
     6961 atctaaaatt tcttctgctc ggctttttta gaaaataacc tgcttctgtc aagatcattt
     7021 tgcggttttc gcgtacaaac agttctgttc ctagttcctc ttctaactcc ttcagttgtc
     7081 ggcttaaggt tggttgtgta agatgtagaa cttctgcggc ttgagagatt gttccttctt
     7141 ctgcaattgt ccaaaaatat tttagtaagc gtaactccat agcgatgctc cttgattttt
     7201 ttacttgata cattccattt tatttctaga taacacacga tcatatttct tgatttttcc
     7261 ttgtaaacgt tccaaacact cttgcatttc agcaatttct ttcgtcaaaa gttcactttc
     7321 ttcaatcaaa agcttacgcc gctcaaatac agttgcttct ccttcgctag ataggcgaat
     7381 atattcaatc aaagactcga tcgataatcc ggcatttcgc atacagatcg taaagtcgac
     7441 ccattttagg tcttcttctg tataatcacg gattccgcct tttgtccgat taactggtgg
     7501 aatcaacccg attcgttcat aatagcgcaa tgtatcggcc gaaatgccct tttcttcact
     7561 gacttgtttg atattcatct gatcactctt tctcttaatt tcttgccact ttacttatgt
     7621 attttattta ttctacaact ttaagtgtac tccaagtcaa tactcatttc tattaaaact
     7681 aagctggatt atctatatcg gttgtttgtg tattatttca aataaaaaag tcttgttttt
     7741 ttattctttt ttggatatac ttttaacaat ttagggaaat tttaggaaaa ggggtataaa
     7801 aatgacaacg tatcagcgaa aggtattatt atcgacatcg gcgggaattg ctttagagaa
     7861 tatggatatt atgtttttag ctttttcatt atcttcaatg attacttctt ttcatattag
     7921 tggtacgcaa gcgggtttca tttcaacgat cactaatttg gggatgctca ttggaggtat
     7981 tttttttggc atcatagctg ataaatatgg acgtgtgaaa gtattttctc aaacggtcct
     8041 tctgttttcg attgcttctt tattgatgta ttttgcttca aacatctact tggtttatct
     8101 ctttcgcttt atcgcaggtg taggagcggg aggagaatac ggggcgtgta tgtccttgat
     8161 ttccgaaagt ttttcaaaaa aacaaattgg acgagcttct tcggttgctg gtattggtgc
     8221 gcaggtcggt gcggctttgg ctgcgatttt agctgctgtg atcattccaa cgtttggttg
     8281 gaagatgttg tatgtcgtag gtgtactgcc ggtgttgatg gtcttgttca ttcgtcgcgg
     8341 cttgactgaa ccaaaggaat tcaaatctgt acaatctgcc gaaaagaaaa ccaaacttag
     8401 cagactgttt gaatcaaaac atcttgcttg gcaaacatgt ggccttagta tcatggtaac
     8461 tgtccaaatt gccggctatt ttgggttaat gaattggctg ccttcaatta tgcaaaatcg
     8521 attagggctt tcggtttctg ggtcttcatt gtggatggtc agtactattt taggcatgtc
     8581 tttggggatg atgacttttg gcataatcat ggataaattg gggcctaaac tagccttttc
     8641 tttgttttta atttgttcat ccttatctgt ctatctactt gtgttagccc atagccagtg
     8701 gagtttggta gcagccgctg tgatagtcgg ctactttatc aatggcatgt atggtggata
     8761 tggcgcagtg attagtagtt tgtatccaac agaaatccgc gcgacggcga ataatttcat
     8821 tatgaacctt ggacgagctg ttggtggttt ttcttctatc gtcatcggtt tcttgatgga
     8881 tcattatagt ttaactgccg taatcttgtt tttgagcggt atttacctag tcagtctgct
     8941 ggtcttgaca actcttactg gtgttacagc attaagggaa agcttgcagc attgatagtt
     9001 gagtctgtaa atgacaagaa agaggtgtgg caaaagcttg catctgcggt aataagacca
     9061 gccaaatcaa aaacatgaga cactgtttcc gtttggctgg tcgtattact tgatgcaggg
     9121 cagctttttc aactgctaat ttccttctat ctggcggcga ctattctttg acgtccgtca
     9181 tttcaaagta ctttactaat aacttagcat aacgaaaaac tgtggataca ctcgtatatt
     9241 gagcgtctgc ataatcttcc aaatttttct caggatgttg atacatgaat tcaatcaaat
     9301 gataattata tgattttctg tagaaatgaa gataaataat gtagattggg aagttctcct
     9361 gcttttccat agaaaagcgc cgattattct cattcttcaa aatgacctta tcaggatatt
     9421 tttggaacaa ttcttctaaa tctgttagat aattcaagat agacttactt gataccttca
     9481 attcgttttg tagttcttct attgaacacc aacgttctgt ttcaagcagt aaatgaatca
     9541 ttttaatttt cttttgagaa acttttgtta aaaaagtctg catattttct cctcctgtat
     9601 ctactttact atactacata caagaaaaaa aacggataca aatatcatat tgtcacaccg
     9661 tcaatagtgc ataaacctgt tattatttct ctttttttcc taagtattct attgatatga
     9721 atcatttaat cgtttattat ataaatgtca ataatctaat aataaaataa cgtacatatt
     9781 ttattattgc atacttaata acagaactat agcaaaaaat cccgtgtatc aacacgggat
     9841 tagattaagt ttcctcaatc actattcata acacaattat tataacaagt aagtaaacaa
     9901 ttgaaaagga catttattga aaacggttgt tttttactta aggaggaaat ttagcgaaaa
     9961 aattagttgc attgtttttg gtcgcaggaa gtttgttgag tgggtgtggt gctactacgt
    10021 ctactactca aagcacaagt tcagatcagg cttccagcag cagtaccacg atctccgaaa
    10081 gtacggcaga ttcatcgaaa gaacaagaac aaattgaaaa gaaaaaagaa gagaaacaaa
    10141 aagcggaaaa agctgcacta gaaaaaaagt gacagaagcg gaatctgctt tgtcacaagc
    10201 tgaagcaaat cctacaaaag aaaactatgc aactgcttca gcagctatcc aagcgctacc
    10261 tgaaaacaaa caagagttga caagccgtct tgctgcaatt gattccacaa tcaaagcaaa
    10321 agaagcagaa gaagaacgga aaaagcaaga agaaattgca cgacaacaag cagaacaagc
    10381 gcaacaagaa gcaatcgcac agcaacaagc agaacaggcg aggcaagaag caatcgcaca
    10441 gcaacaagca gaacaggcgc gacagcaatc agcagctgaa caggcctctc agcaggcaca
    10501 agccgcagct cctgagcaaa ataatgaaca aactgtctat gtcacaccga ccggttcaaa
    10561 ataccataca cataaatgtg gaaatggtac gtatacccct gccactttaa gcgaagctca
    10621 agctcgtggt ttaacccctt gctccaaatg ttatggaaac taattcaaaa gttatactat
    10681 aataataaaa ttcttctgaa acataaaaaa atagaagatg gcgttccaga tagtaaacca
    10741 aaaataacag tttacacgtc catttcgtca gcttctctct tttttcattt agtctgttcc
    10801 ctctgctttt ttggacatct tcattcattc tttctcaatc aaatagtaca cctctttttc
    10861 ttttcgcagt ttatatcttc cttttttcaa tttcattgaa aactgtttcg aatgtttatg
    10921 ttttacatgg attttaagca ggagctcctc atgttgcaag cgatagttcc tgccttttaa
    10981 atcagaaaat ctcactagtc cgtattcatc agaagctttc acaaatggaa ctttttctct
    11041 agtaatatag tgtttgccgt tgagcgtttt tacagaaaag accattcctt cgattggtcg
    11101 tccgtttgtg ttctttacat aaaaagataa aggatagttt cgcttagcga tggccaaaga
    11161 atctaaataa gcgatgagta gccaaactaa gccgattgat ccaagtagta taccgataaa
    11221 ccaaataaaa aatttccatt ttttccattg atcaattcct tggatcgctt tttttgcctc
    11281 ttttgcttga taaggaatac gatgccccgt cacaagcagt cgatgtgtat tgaccatata
    11341 aggagtacag gtaagcaaag tcacgagatc tctgcctttt tggataccca aagaatctac
    11401 ttcatcaggt aagaccactt ggattttttc tacttgatac gccaatgtct ttccattgat
    11461 ctggatgaaa aactggtctc cttttttcaa cttaggcaaa tctgtaaata attttgcttc
    11521 tgggagtcca cgatgcccag aaagaacggc atgggtgttt ttcccgccag tcggataaga
    11581 actttcttcc aataatgacg ttcctttttg caaaaatatt tccgttgtct gatcgaatat
    11641 aggtaagctg acattgattt ttgggatttc gatgactcca agcatatgtt ccataaaaaa
    11701 ggcattacta ggtaaatctt ttaatgcttt tgcatcaact gcttggttga aactgcctat
    11761 ccctggtgat acgttttgct ctgccaactg ttggtttttc tttgtcattt tttcctgctg
    11821 tttttttatt tcctctgaat tcttattcga tgcttgcttt tgataatgga tgatcatttg
    11881 ctgggcaaga aaatcattca gtgaatcttc tacaaaagga taagaaaaga tcccgatccc
    11941 aacgactaaa atgattgcca tgaagatgtc cagtattctt attctgcttt tattcatcat
    12001 gtccacctat tttctaatat gtatatgaag aggttgtgaa atcagcgcct tgacctgagt
    12061 attagcgaag caaacggcaa aatagttcct cctattttgc cgtttgttga gcgaatacca
    12121 agagaccgac cacttgtcac aacctttctc cccatttgaa tcactatcgt ttatttatcg
    12181 tatgattttt gtttccaata gtttaagctt ctgtttcttg atggcgtttt ttgaaataga
    12241 tcactgctcc tccaactaaa gcagtaccta ccaagatgaa tacgatgatc cccataccac
    12301 cagttgaagg caagattcct ttaggttggt tatcgatcgt tgtttcatca gaagttgttg
    12361 gatcatcatc tactgtaaat tcagtgtacg cagcttcatt cttaggtaat gcgtagcctt
    12421 ctggtgcttt tgtttcttga aggcagtatg ccccttgttt cagcccttcg attgaaaatt
    12481 tcccttctga atcagaaatc aatttcactt cttcccagtc agaacctgtg acttcatctg
    12541 accaagcata tgggtattct cctgaggcaa ggtttgctat ttttttcatt aatttaacag
    12601 ttggatcttt tgctcttaga atagaaaatt ctgcatcggc taatttttct tgcgtgttca
    12661 catcttgttt gatgaagtgg aaatcaccgt agttttggac attttctaca gtaatcgtgt
    12721 tatccgtagt tgtattttcc cgattgacta caccgtttgg attcccaaga ttggcttcat
    12781 ctgcgccatg gatgataccg gttgtttgtt gcaattttcc gttttttact ataaaagtag
    12841 tttctttttg tggattggtt aattgataac catctggtgc ttttgtttca actaaagtat
    12901 aattgccgtc tattaatcca ttgacactga tggatccttc agtatcagtt gtgaatgtca
    12961 acgctgactc agcaggatca tagagagcga caccagtgtt ttcatcaaaa ccggtcacga
    13021 attgattctc tgaatttttg atctggaatt gcgcccctgc taacttatct acccctgaaa
    13081 agttcccagc tttctggatt ttgatattgc tttctttgat gacattttta gcatataggt
    13141 gaatcgtgtc taaaggtttg tcagggtcta gggtaagctt aacacgatat ttggcgacgt
    13201 ttcgacaccg ggctttggtg tttctacaaa gacgtataca gcatctttcc catcagattt
    13261 acctggaaga gtcaggttag ctaatccttc accagaagtc gttacacttg cgaagggtgt
    13321 agcgtctgct ggaactgctg tgtctttact tggatagtac gtagcagtga tgtcatatac
    13381 gttgaattca acgccgttca acggttcgct attcggaaat gtcaattccg ttccgttgtt
    13441 tatttgattt tctggtgctg aagtgaattt caatttatgc aaattaaccg taacattttc
    13501 tggtgtaggt gatacggctg cagaaacctc tgcagttcct aggccgagaa cgaaaggaag
    13561 cagcatgata gcagttccta aaaacgtcca tatttttgtt ttgatgtttt tcattttatt
    13621 tccctcctat tgtttccttt ttgttgtttt ttataaaacc aagctgtccc tgttgaaagg
    13681 actagcaata accctaaaat tgagaaacct ccaagcatct cttcgcttgt ttttggaaag
    13741 cttcctccat ttttttcatt tgatggacca tttgtctgca tctgaactgg cgtttttcca
    13801 ttattcgtcg gatttgcttt tggttcgttg atgatttcca atggtatagg atgaagcgga
    13861 tcataactgc ctttttcaac ttcaaagaga atggttgatt gactcaatat atatccttta
    13921 ggcggtttaa tctccactag ttcgtaactt ccaaaagaca atccatggac agaaaatgtt
    13981 ccatctttac tggaagtaaa aatcgttgct cttgaatcat ctttggctac ccaatgattt
    14041 atcccgtttt cttgcgctaa gtattggtca ttttttttga ctagaaattg agcatctgct
    14101 agtttttgtt tcgtttgccg atccactttg acaaattgtt tgcctcctgt ttggatggca
    14161 agatgtgttt ctactgtttg attgtcagtc gtcaaacgta catcgttatc aaatgtcgtt
    14221 ttttcactgt tttctttcaa tgtcatttgg taacggatcg ttaatttttt tccagcaaac
    14281 gaactaagat cttttccaga atctgataag acaaaaccat gttttatttt ttgtgtgtga
    14341 aatgtatgaa tcttgtctcc accaatcaaa aaatcgatcg tttctggttc tagccataac
    14401 tgaggatcag ctgtatcttt gatcgtatag gttcctatct cgttgatgtt ctcaggaatt
    14461 gttacggtga tgagataagg gactttatcc ccatctgcaa aatctgtctg cttattctct
    14521 actgtttttg ttaatgtgag tgcagcaggt attttttgtt cgctctttgg atacaagtgg
    14581 atggtcgtca atttatgatt agatgaatct gtcacaggaa gcacaacgac aaatggtgct
    14641 gatttttctt ttacttaatc tgaagtcttt atttccgtaa atcggtaaac agcgtcacga
    14701 ccttgctcat ctttgtccga tgcactaaaa gaagcaatgc cctcttcctc gtctactgtt
    14761 tttgtcacat tttctgccag tattttttca ctatcacttt tttgtgctag ttttctttgt
    14821 gcgtcttcca cactacttcc ttctgaccgc agcttgtaaa attcatccga gacatcatat
    14881 atagcgaaag taacgccgtt caaccctttt actccttgaa agttatccgg gatatttatt
    14941 ccgtcgtttt gactgatact tggcatctta tcgctttcga acattcgatt atgcaaagta
    15001 aatgtaatag gtgattctct agctgacaca gtcggagaac ctataaaaaa gaccagtagg
    15061 ataaaaaaga gaacagcaaa tatatccaca cgtacatttt ttgttttttt catcgctgtt
    15121 cacctgcttt tttcttacga tggataaaat aaaagaagcc agaaaaaata aagatcagtc
    15181 ctaaaaagac aaagggcagt agacctatcc cacctgtcga aggaagcaag agagtcggat
    15241 tgatcgtcaa cttagcggta tctgttgcga taatcgtata attattgttt acacttcctt
    15301 cgcctaaact aatcgttact atttgtgatc caatatctgt ccggctgtta actgttaggg
    15361 tataatccaa tgattcgcct aagacaacac cggtaacgtg gagatttcca accgaaatcg
    15421 agtgactttg ggcatcataa ttaaaggcag cgtcatcgac agtaatcact gcgttagctt
    15481 tatagatggt ataagttcct gatgtgttgg ctatatcagt aaatgacaac ttgggatttg
    15541 ctgctttgac tttgtctatt ccgctattcg ttagtttaat tgtatagctg ccagcattaa
    15601 ccatattttt tgctgattgt gcatctgctg tgtaatccat actgctaagt gccacttgta
    15661 ctggagtaag cgaactagtg acaccctctg ctagcttgac atggattgac gggacacctg
    15721 cttttccatc atatgtctta ctgccactga gctctaccgc accgctgatt tgcttaatcg
    15781 tatagacatc agatgtctgc acgatagtaa ataaatagtt tgttctggca gctagtttag
    15841 ctagtccgct ttttgtcaat tgccaagacg tataggttcc aatttcagta ggcgcagtta
    15901 cttcaactcc cttttcattc atcgctttga aatcagttgg attccatgtg atttgatcaa
    15961 tactgagatc attgtttaaa agggatgttg attctgtttt atcattaaag gcatatttaa
    16021 cgatcggtac aacagaaaga ccatcatagt acttactgcc atttgcattg attgatactg
    16081 ttgcatttgt cttataatat aggatgactt cagaaatctg ttttccattt gcgtccttta
    16141 cgattacatt tccatttgta tctaaactag ctaaaacact cattgctgat tggttctttg
    16201 tttctatcgc tgaaacagct gtaaaggtat atccgttgat agtaggcgta ttagtcgtca
    16261 ggtcttgtgc agcacttccg taagtaagat tcattatttt agaaggagca atcatctgtc
    16321 caaaatagtc ttgatactta atagtgattg gtgtttgttt gcctgtatag gtcaccgtat
    16381 cgacgttact ttctagggct gtgaaagaac cgtttgtttg tgcaaaagaa tagcccgtta
    16441 ctgctgttgg agtgataacc actttgtcac ccacatggat tccagataat gtagtcccgt
    16501 acgttccgat ttttgctgct ccaccattga ctgtataaga aaaagttcct gtccgattcg
    16561 cattgacctg aaccgttgct gcttgatagg ttggaaaatt tgaaacattt cccttaccat
    16621 caacgatctg cccatttttg atcgagtacc ccgtgaatgg aatatcattt gctaactgta
    16681 taccgattga aaaagtactc atacctcctc cggcaggaag ccctccccat gttgagggtg
    16741 taaaagaata acttttatcc gcattgggtg taactgaatc gatcgtattt ccagcggtag
    16801 ccacccccat atagatactt tttgggaatg tgatagtagt accatttact gtggagccaa
    16861 taaacagatt atggatatct ggacggttat caataaaagc gattgctttg gttacgcctt
    16921 gtaacgcagt tttatccgca ctcatcgatc cgttaaccgc aacaatgact ccatctttgg
    16981 catatatcac tgtatatcca tctggtatgg tatgtccgtt gaaattcgca gtggccgcaa
    17041 cagtactaaa tgtctggctt ctacctgttt ctgaataagc tttctcccct gctgcttttg
    17101 ctgctgtgat ttctttttct gtcggatcaa tcagttgagt cgaggatgtg gttgaggttg
    17161 tctcgttgtt gctagactga ttggtgcttt ctatggactg tatttcagaa tagggtggac
    17221 tatttgtctc ctctgtcgtt tggttagcaa aatttgacgt ttctgtataa acgtttacat
    17281 aaatacttga taagctccct aagaaagtaa aaatcaagat gatcgaaatc ataatgggat
    17341 gtaagtattt tcttttagat acagctttga gtctagaaac aaaatgtttt tgacatatac
    17401 gttggatttt attttgagtt ttcttaatca ttttcatccc tcctctcaat aaaaaatcat
    17461 caaataatcc tttacacgtt attctaaatt taatttttat tattttattg atgtatagaa
    17521 agtttggggc tttttttctc tttttattct aaggttattt tagtcttgtc ctttaataca
    17581 aagattttat ttttaaataa aaaatactct ttttttacga ttattgaaaa aaaaagataa
    17641 aacttgatta attgaagtat tgcttaaatt tggaagctat gagggaagaa taaaagagtt
    17701 aggacgggat tatctactat ttttctttgt tttaaatatt tgttatcttt gaacataaaa
    17761 gagaaatgtg tcgactgcag tgttgtgtcc ctcatcacga ataaacggtg ttcaactagc
    17821 tgatcttgta ttcacttaaa aaatggtaaa gtacgagttg ctattcccca ttttcaattt
    17881 tctaacgctc aggacaaaat actgttttca caacctcatt ggaataaaag catatatatt
    17941 gtttcaagaa cgaaatcttg ttatcttttt aaagggcatc tgtccaaaat ctaattttag
    18001 gaggtacgtt ttggtattta ttcgtacaaa taaaagagaa ttatctttat tcatgtaacg
    18061 catccaattg attttttcat gtacgctcct ggaacgattt tactgacgat tgcttattta
    18121 actgcgaatc gctcattagc ttttatcata gctatccata ttttgaataa cgttcttggc
    18181 tttgtattat agatacagat gacaaaaaaa gttcaagtat gcggtgtaga accaatatcg
    18241 catacttgaa cttttttcaa tgagtataaa ttaaaacttc taattttcaa ttcccatcat
    18301 ctgatatgcc cgaccgtctg aacttctttt gaaaaagcca aatgtgatta gataacgtct
    18361 ttgttcgaca taatcgccac tagaaaatac gttcagcagt atcgaattaa cctctgtttc
    18421 tgtataagtc actccatggt caaagcgttt caagacttct ttcaatagga tgtatttctt
    18481 ttttgttgaa cttggtactt gttttacttt tccatcattc atgaatgtgt ctaaaacttt
    18541 catcttttct tgctccgtat aagtataaac catcttcttt ctccttttga ttctaagaat
    18601 actccattta tgttagaata ttctggattt tttattaaag cggttactct taagaaagat
    18661 attatcatag tgctcttttt tttactacta cttataggta gatttattct aatccattat
    18721 tctcccctac caataattaa agaaaaaact ggatcagggt gttcaaatta catgctaaga
    18781 aaaaagaacc cttgaatacc aagggttctt tctcagtctg ataacaatga ttaacgacgg
    18841 atttctttga tacttgtgaa aagtcgttaa agtattgata taaaaggatt tttattacct
    18901 ctgactacat attgactaat ttgttaaaaa atcgtttttc aagaatcctg taataccagt
    18961 gctttcaccg tttttttaac tctctaaaga actaaaagca atcagaggtt ttaccttttc
    19021 atttgacgtt aagtggcgat aaattttcga agttgtttgg tcagtatggc ctaagcgatc
    19081 tttcaagact tcccagctac agtgttccgc tactaagctt gccattgtat gacgagctaa
    19141 atgagtatga aatggcttat taattcctga gcgattcacg caccgtttga cgtgatcatt
    19201 tacatactct cgacgataag gaacaccacg tttactagaa tcaagataca caaacaaata
    19261 tgtttcttct ataataaacc tgtaatgttg cctcatctca cccaaagcct taatcaactg
    19321 ttgtagcttt tccataacta gaggagttac tagaactcgt ctagcagaac taactgtctt
    19381 agtagtttca attcgaaaat ccttactctt acctttaact gccgtttgct tatagaacag
    19441 aatttcattt cttttgaaat ctatatctcc aggctgtaga gcaagcgcct cgctgactcg
    19501 gcagccagtg taaaatagaa catggaaaag tacagacatc ggtaattcct catgaacaat
    19561 gccataattc aaaaaagttc tcaattcatc gacagtaaga aacttgtcct caatattatt
    19621 tatggcttct tttaaatcag ttaccgttaa ctgatatttt ggcattctca cagacttcat
    19681 tggattttca cttatcaaat tctttctttg acaatattta aaaatcatat ttagaacttg
    19741 aataagtgat tgtaaactat ttttggcata gccaacattt gttccattaa cgccacattc
    19801 ctttaaggta aaaagcaatt cttgaaggca ctctggtgtc aaatcagaca actttttatc
    19861 accaatccat cgaacaataa tttttaattg ttcctctcgc ttatcatagg ttatttcctt
    19921 ttgttcattt aacgaacggt aatattggaa ccattgctca cctacaacac taaacttgtc
    19981 attgcttgca acaatttttt tcttaagcct ttgttgtttc tccatttcca gataagcgag
    20041 ttgcgcctca cgttttgaat taaagccacg tcttgttgtt cgatatgctt gaaagtacca
    20101 tgtgatccta ccagtagaac tgatttgctt cttgaatcga tctgtttttt ccatttattg
    20161 attcctccta ttttgaagga acccaacaag cagaaaccct ttggaaatct ttctatctat
    20221 gtcatcattg taccgcatct tagaaagaaa atgtaactta taaagatcga agtagtcgca
    20281 atttttctcg atagtcattc gaatcactct ttctcatcca ttctaaaaca tcatcacgaa
    20341 aatagtgtcc tcgaatcccg taccaacagg gaggaaactc accaagtttt actaaacggt
    20401 tcagtgtact atcacttatc tgaaatagtc gctttaaatc agtatttgtt aataccagag
    20461 gtaaatgact gactgcaagc aatttttccg tgtaatcttc cagttttttt tgtaaaattg
    20521 aatagaactc tatttccaga gaattaacat ttgttttaac ttccaactcc atcacccttc
    20581 tctctcaaaa tcaaaacgac caccacggta ccccttaaaa ttgtaaaaaa acactccagt
    20641 actccatcaa aacggactag aaaagaggaa tttttaatat gaaacaagga attgcagaca
    20701 taaaaataat taaagaaatt cttgaaaaat ctacggctaa tgcaatagca ttcggaacag
    20761 gaatcaactt gagtacagta aaaaaactta aaagtggaga gagagctgaa gaaaaattaa
    20821 atcttgccga tgctattaaa atcacagaat ttggcatgaa aaatatgcca accaaaatcg
    20881 aaatatggaa atagtagttt ttctgatcta tcagctgttt tgccaaagca cataggaatc
    20941 tcacctcccc ccagtaatgg cgggccgatt tggttacgga agtatcatta tcgtagtcgc
    21001 gattctcata ttaaaatttg tcgcttaaaa gctaaccttg tcatggcgta actagagaat
    21061 caaaatctac atgtactgat agatttgcta ttcatttgtc aaagagcgct aggctatttt
    21121 cagcctatgg aaatccttta ttttgacccg ataaaaaact tccatagaat gaaaaggaaa
    21181 gggaaaaaat actttaacga gttgtatcaa attcaagtat cttctcaatc aattttgttt
    21241 ccagttgtct acgcatatat tcatctacat acataaccat tcctttttct tcttcataaa
    21301 ctggtttcat ggctaaccgt gaaatataat tttgatattt tgacagaacg atggacatgg
    21361 caattgaatc accagaagct gcggctttga ttgtcgttag tggtatgaat ggtatatatc
    21421 gtttattttg cttcatattt gacacccaaa tactttccta gctcttccaa agccatttgt
    21481 cgttggtacc aaacactgcc gagacttttc ccaataatat cgccaatttc acgatcatta
    21541 agaccgatga agaaggacat aaaaatgatt tcttgtcttt gactaggtaa tgctgcaatc
    21601 gcatttgcca actcctcatt ggaaataaag attttcatac caaggacaat aaaagtacca
    21661 ctactattca aatttggttc caaataatag aactctgtac ctaacccttt ttgttccaat
    21721 atttccattg atatttgccg ttggcgaaat ctagcgtact tcttttgaat atttaacgcc
    21781 tcattgcaga ctactttctt acaaaaactg tcaaacgtat gttgaatgtg ggtttcctct
    21841 gagtcgccca taatattctc cccctcaaaa aattcattca actcgtttta cttctatccc
    21901 tttccattat tactacaatc tagga
//
LOCUS       DACEPD010000048        21498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.049, whole genome shotgun sequence.
ACCESSION   DACEPD010000048 DACEPD010000000
VERSION     DACEPD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.049"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            5..403
                     /locus_tag="IW797_001667"
     CDS             5..403
                     /locus_tag="IW797_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ1224450.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="IW797_001668"
     CDS             387..1082
                     /locus_tag="IW797_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ1224451.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1666..2553)
                     /locus_tag="IW797_001669"
     CDS             complement(1666..2553)
                     /locus_tag="IW797_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ1224452.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2738..3577
                     /locus_tag="IW797_001670"
     CDS             2738..3577
                     /locus_tag="IW797_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1224453.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3773..4747
                     /gene="sgrA"
                     /locus_tag="IW797_001671"
     CDS             3773..4747
                     /gene="sgrA"
                     /locus_tag="IW797_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ1224454.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5045..5401
                     /locus_tag="IW797_001672"
     CDS             5045..5401
                     /locus_tag="IW797_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224455.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5404..6561
                     /locus_tag="IW797_001673"
     CDS             5404..6561
                     /locus_tag="IW797_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224456.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6587..7027)
                     /locus_tag="IW797_001674"
     CDS             complement(6587..7027)
                     /locus_tag="IW797_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1224457.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7187..7693
                     /locus_tag="IW797_001675"
     CDS             7187..7693
                     /locus_tag="IW797_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1224458.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7861..9774
                     /locus_tag="IW797_001676"
     CDS             7861..9774
                     /locus_tag="IW797_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ1224459.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9779..10780
                     /locus_tag="IW797_001677"
     CDS             9779..10780
                     /locus_tag="IW797_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1224460.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10982..11188)
                     /locus_tag="IW797_001678"
     CDS             complement(10982..11188)
                     /locus_tag="IW797_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ1224461.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11834..12682)
                     /locus_tag="IW797_001679"
     CDS             complement(11834..12682)
                     /locus_tag="IW797_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1224462.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12846..13748
                     /gene="gnd"
                     /locus_tag="IW797_001680"
     CDS             12846..13748
                     /gene="gnd"
                     /locus_tag="IW797_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ1224463.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13750..14745
                     /locus_tag="IW797_001681"
     CDS             13750..14745
                     /locus_tag="IW797_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1224464.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQARVKDDRWEDRAEFVGRTLFNKTVGVIGAGNT
                     GSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTEEN
                     YHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQDH
                     PYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14742..15026
                     /locus_tag="IW797_001682"
     CDS             14742..15026
                     /locus_tag="IW797_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1224465.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15059..15856
                     /locus_tag="IW797_001683"
     CDS             15059..15856
                     /locus_tag="IW797_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1224466.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15870..16703
                     /locus_tag="IW797_001684"
     CDS             15870..16703
                     /locus_tag="IW797_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1224467.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16721..17578
                     /locus_tag="IW797_001685"
     CDS             16721..17578
                     /locus_tag="IW797_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1224468.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17600..18109
                     /locus_tag="IW797_001686"
     CDS             17600..18109
                     /locus_tag="IW797_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1224469.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18146..18547
                     /locus_tag="IW797_001687"
     CDS             18146..18547
                     /locus_tag="IW797_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224470.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18659..19012
                     /locus_tag="IW797_001688"
     CDS             18659..19012
                     /locus_tag="IW797_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224471.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19046..19489
                     /locus_tag="IW797_001689"
     CDS             19046..19489
                     /locus_tag="IW797_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ1224472.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19506..20591
                     /gene="uxuA"
                     /locus_tag="IW797_001690"
     CDS             19506..20591
                     /gene="uxuA"
                     /locus_tag="IW797_001690"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ1224473.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20584..21297
                     /locus_tag="IW797_001691"
     CDS             20584..21297
                     /locus_tag="IW797_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ1224474.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         7397 a         3300 c         4490 g         6311 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta cttatttctc atttaaacga
     1201 tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt tttgaataaa
     1261 tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc tctagtataa
     1321 cagaataaga ggaacaagtc cagaagggat atttttagtc ttttttaaaa taacttttca
     1381 gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa aaaataggga
     1441 agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa aaaacttttt
     1501 taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca aataaaaaaa
     1561 ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga atttccaaaa
     1621 atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt tgaatatatt
     1681 ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac ggattgtttt
     1741 atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta gctcagattt
     1801 gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta gataatagct
     1861 ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc cgttttttag
     1921 tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat caaaattcat
     1981 ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa gcttggagaa
     2041 attttcccct tcatttgctt ctttttttgc tttatctgct tcttctttag tcgaaaatag
     2101 aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta gatctttatc
     2161 tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt taaagagttt
     2221 cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt ttggaccgag
     2281 ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct cttttccgta
     2341 tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat tatttgattt
     2401 aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa acgttgcaat
     2461 cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg aaaggcacat
     2521 aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa cagtctaaca
     2581 gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt cttagttcgt
     2641 aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga caagaaagaa
     2701 aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta ttcggttgat
     2761 tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca ataatgcact
     2821 tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta cggtcggaca
     2881 tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa aaagaataga
     2941 agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag gtctcgttgt
     3001 tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg cgtatcggtt
     3061 agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat ttattgcagt
     3121 gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc ttagatttgg
     3181 tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg gattgaatcc
     3241 taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt tcagcgcact
     3301 tatctacagt acaggaagtt tatttacggc tattggtgct catattactt ggaattggac
     3361 acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt ttttctccac
     3421 tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg aagcatctat
     3481 atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg catataaaac
     3541 aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag caaggctgat
     3601 atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca aattcaagcg
     3661 aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca tcattttcat
     3721 tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag aaatgaagaa
     3781 aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa tgattggaat
     3841 cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga aaactgatcg
     3901 gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa acaaaagcgt
     3961 gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg gaagttcaga
     4021 aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta ttgagtatac
     4081 aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata aagttgattt
     4141 gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa gcagttggac
     4201 agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta cagaaaacac
     4261 gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa acacgagcag
     4321 cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga gcagcgaaag
     4381 cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg agagtagcac
     4441 aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg atttggtacc
     4501 tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa gtgtgctaaa
     4561 acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag aaacagataa
     4621 tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa ttacatggat
     4681 gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct tccatcgagt
     4741 gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt tttgagtgaa
     4801 tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag atctattttt
     4861 gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa ataagagcaa
     4921 gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt aacgaatcta
     4981 tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa aaggagtatg
     5041 tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat ttttgaacta
     5101 ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag atctaaacta
     5161 caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg aaaggtatcc
     5221 agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt tttcttctca
     5281 gtttttactg agtaaagttt attctatact tttagaggga acgattggtt atattttact
     5341 ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa aaattatata
     5401 agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa aaactatcat
     5461 ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg tatccgatac
     5521 ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat attatcaagt
     5581 ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata tacgcgtagt
     5641 atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat gaaacaaggc
     5701 cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt gataacaaaa
     5761 gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg gtcgatgaat
     5821 cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat gaacacctat
     5881 ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg ctggattgaa
     5941 tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac ttcagaaaaa
     6001 aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt tgggacaaaa
     6061 aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca tatatttcca
     6121 gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat tccagagctt
     6181 aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt agtcagggat
     6241 atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta tggtaaaaca
     6301 caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat aaaatatagt
     6361 gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga caaaaaggca
     6421 tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa aaatgattgg
     6481 gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc catattggaa
     6541 aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa gacattgcaa
     6601 aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg ttgagctctt
     6661 gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat ccagtcaaaa
     6721 gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat gtacgaatct
     6781 caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt ttataactaa
     6841 tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt aagtggataa
     6901 attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt atatcatgga
     6961 cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta tacaatttaa
     7021 tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa gaaatagtcg
     7081 aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg gaactagcta
     7141 gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt caaacacagc
     7201 cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt cgccgataca
     7261 cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg aaacgattgg
     7321 gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa caaaaccatg
     7381 ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa aaacccatgc
     7441 gaattcattt tataagacta atttgagtgt tttattaact gaattggact gtcattcgat
     7501 cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt ttgctcatgg
     7561 attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata atccttttat
     7621 gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat ttctgacatt
     7681 tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg gtttatccat
     7741 tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg ataaaccatt
     7801 tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac ataaaaagga
     7861 gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt atttgctgta
     7921 agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga atcggaatct
     7981 cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc gagtacttca
     8041 caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac agatcaagaa
     8101 acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa aatgtcaaac
     8161 gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa tgatgatatc
     8221 ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa acagatgaca
     8281 gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt attagtcact
     8341 gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct gattgctact
     8401 tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat gcttggaggt
     8461 acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac aaaacaactt
     8521 catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc atattatgtc
     8581 gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa ttggaataac
     8641 gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt attatttgtt
     8701 gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca atatgagtca
     8761 gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca aaagatttta
     8821 ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa agatattcct
     8881 gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga acaactacgt
     8941 tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa tcagttagac
     9001 atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca atactatttt
     9061 gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat gtcagattca
     9121 ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc taacgcgcaa
     9181 aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg agcagggcat
     9241 ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga ctttttgaaa
     9301 gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa aagtctactc
     9361 aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat gacagagcaa
     9421 aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg gatttttaat
     9481 gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc accaaaaaag
     9541 caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga ggatcaatca
     9601 agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac agatgcaaaa
     9661 aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta ttcaggtgga
     9721 tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg gtaaatgaat
     9781 gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta ttttagtcac
     9841 tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta gaaaacaatt
     9901 tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc aaaaaatcga
     9961 acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata ataatatgga
    10021 tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga tgcatggagg
    10081 cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc tatgttcaga
    10141 cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa aacatcctac
    10201 accagttatc caattgaacg aagccattca agcagctaaa aaagagataa aagatcaagc
    10261 agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta gtggagaata
    10321 tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc cgactttgga
    10381 gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt ctgcacttac
    10441 tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg cttactttga
    10501 taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca gacatgcgga
    10561 tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta cgaaacaaat
    10621 ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag tagattatgc
    10681 ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata aacaagcgca
    10741 agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga tgcaaaagag
    10801 gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac aagcttttca
    10861 tggtttatga atattgtaac ttgtgactga ggagttagag atggcaaggg gcacctttta
    10921 ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta aattttataa
    10981 gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct gcggaggcaa
    11041 gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat attccgttgt
    11101 tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaatgca ctagcgaaaa
    11161 aaagcaaaga aatatggata gctttcataa gcagcacctc cttttttata attgtatgat
    11221 aaatagatta agaatctata aatgtgacaa gtttgttagg atgtgaataa aatgatcatc
    11281 tagcgaactt gaaacaaaga tggctaatga taagcaatta atgattcagc aatcagatag
    11341 gtacaaagtt taccatcata cgtctagacc aataatagaa aatatgggaa tataatagaa
    11401 aagatttagg atacccccaa aagaatttcc ccaaaaattc gatgtatcct aaactttttt
    11461 attttacata acaatcatat caactgtgaa ataaaattac aaatgataac ctaaaatgag
    11521 gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt taatttttta
    11581 gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact actgtatact
    11641 aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct tctccaaaga
    11701 tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc ttgcagaacc
    11761 aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat ttttcgcttt
    11821 ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta gcgatagaac
    11881 gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc aaaatgctta
    11941 gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca gaaatagaaa
    12001 taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta ataacgattg
    12061 tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt gattgccctg
    12121 tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc atcaattgaa
    12181 tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta tgataagcgt
    12241 cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta gattcattga
    12301 taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg tcatttaaag
    12361 ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct gttttctgaa
    12421 tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg gccattttaa
    12481 aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta gagtcagcaa
    12541 tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt ggattttcta
    12601 aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt gttttgattt
    12661 gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt atcatttttt
    12721 ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca aaaaatattt
    12781 ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga aatggaggcg
    12841 ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta gctttaaact
    12901 taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct gttagtcagg
    12961 caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct acttttgagc
    13021 agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac atgttaagaa
    13081 aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac tcttattata
    13141 aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac ttggattgtg
    13201 ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt ggaggagata
    13261 aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa agaggatatc
    13321 tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat ggaatcgaat
    13381 atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt gagtatgctt
    13441 ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc ggctggctga
    13501 tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat cggggaatcg
    13561 tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg gctgttccag
    13621 ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa gatagttttt
    13681 cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata gttgaaaagg
    13741 gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt cggaagagtc
    13801 tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt tactgttgat
    13861 caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat cattattgca
    13921 agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact gctattgatt
    13981 tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga acaccagaca
    14041 atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa taatataact
    14101 aatttgttgt ccatgcttcg acgcacgaca caagcaagag tcaaggacga tcgatgggaa
    14161 gatcgggcgg agttcgtcgg taggacgctg tttaacaaaa cagttggtgt gattggtgca
    14221 ggaaataccg gaagcggagt aatcgaaatt ttgcgtaacg gttttcgctg tgatgttcta
    14281 gcttatgatc cttacaaatc agatttatac atcgaaagct ttggtgcaaa aaaagtagaa
    14341 cttcaagaat tactggaaca atcagatatc atttgtttat gtgcgaattt gacagaagag
    14401 aactatcaca tgatttctga taaagaagtc gatcagatga aagaaaatgt ttatctatct
    14461 aatagtgcaa gaggcgctct agttgatgaa gaagcgatga ttaatggtct gaaaagcgga
    14521 aagatcgcag gatatgccac agatgtttta gaagaagaac cgggacgtca agatcatccg
    14581 tatttagcgt ttgaacaagt agtgattacg ccacatatat cggcctacac aagggaatgt
    14641 ttagaagcaa tgggtgaaaa atgtgtcaga gatgtcgaac agatcagtca gcaccttctt
    14701 cctgaacgtt ctgtccagac aaagagcaac tatataaaag gatgatgaag aaaatggtga
    14761 acggtttaaa agtgaaaaca ggccctcaat tttatttata tgaagaaggt ggtatttcaa
    14821 aagtttctga tctcttgaaa tcatacggcg cgaaaagagt actggtcacc cacggaacgg
    14881 tatcatggga aaaggcgcta cctaaattag tatttttgaa tgacgaaacg attcaatttt
    14941 tttatcatcg ttattctgga gaatgtagtt atgctgaagc cagacgaata gctacaatca
    15001 tcaaaaaaat gaaatcgatt tcgtagttgg cgttggcgga ggaaaactga ccgatctagt
    15061 gggttatagt gccaatcttg caagaatcga tttcggagtc atccctactt tagctagtaa
    15121 ttgtgctcca tggacacctc ttgctgtcat gtataaggag aatggacaag cagaagggaa
    15181 aacagaacac ttttacagac aagctgcttt ttttataaca gatccccaat tagtgattga
    15241 ttcccctgtt ccatatttca ttgccgggtt agcagataca ctagcaaaat ggtatgaatc
    15301 tgagctcatc ttagaacaag attttttgca agatgaatca tttttgaagc tagcacaaga
    15361 tactgcgaag atctgcaaag aagagatttt gaatcattct gctaaagcaa tccaagatat
    15421 ggcacaaaga aaactgacat cggagttcaa acagctttca gagattatct ttgctgttgc
    15481 tggtttagtt ggaggattag gagataaata cgcaagaaat gtggcagcac atgctatgca
    15541 tgacgctatc agtaaatata ttccggaaag ccatcgtttt ctacatggag aaaaagtagc
    15601 gtatgggatt ttctatcaac tggcactaga aggtcgctgg gaaaaaatag acgagttata
    15661 tcctttgtat cgggaattag aactgccggt ttctctagaa caaatgggat tatttccgaa
    15721 agataaacag ataatagata aaatggctga atttattgat tcgaaagaga aagtgcattt
    15781 gattcctata gaggtaacac agaaatcact gatacagtca atgatggaac tggaaggtta
    15841 tatcagaaag atctgaagga ggaggacaca tggaaacatt aagtgtagta caaagtttgt
    15901 tgattgcttt atgggtagcc gcggttatgt cccgctggtt aggtggagga gccacgttga
    15961 ccttacgttt ttctcctttg atgactggat tagtcgtcgg gttgatcatg ggggaagttg
    16021 cacaagcgat gattgttaca gccgctttgc aaatgattta tatgggggtt ttttctccag
    16081 gaggatcaat gccagctgaa ccatctattg cagcagctat cgcagtacca gtcgcattat
    16141 taggggatct gtcgccagaa gcagcgattg ccgttgcggt gccagtcgga ttgctcggaa
    16201 gttacttgta tcagtttcgt tttttcatca atactttttt aggtaaatat acagacaaag
    16261 ctgtagaaga tttgaataca aaagctatgg cccgatcaat tattctttat ccaacggttg
    16321 cctcattttt attattcgtt ccgcttgttt tcattgcttt atatttaggg gcgccattga
    16381 tcgctgatgt aatcaaagct ttagagggaa cagtcgttat ccacattctt gaagtagtag
    16441 gtggagggtt agcggcaatc ggtatcgcga caactgtata cgttatcggt cgaaaagatt
    16501 atctggtctt tttcttcctt gcttatttta tgagtgttgt tttgaaatct ttagatatta
    16561 cgatggttac ttatgccatt tttgggatta ttatcgcttt gatctttgtc cagtcgcaaa
    16621 aagggaaaca agtacagcct gccacaagca ataacaattc agcatctttt gatgacgaag
    16681 atgactatga tgacggattt taatttagga ggaatgggaa atggctgaaa taatggataa
    16741 tcaaacaggt gctcctgaag aaatcacgaa aaaagatgta acaaaagcct atctgagatg
    16801 gcattttgca aacgaaattc ctcactcttt tgaacgttac ttggcgccat cactactata
    16861 cggaatgatg ccaatcttga aaaaacttta taaagatgaa gactctttaa aagcagcata
    16921 taaacggcag ttactgttct tcaacactca attaagctgg ggtggcggtg tgattactgg
    16981 cctaatgtct tctatggaac agcaaagagc agaagaagag tatgaagaaa aagagatctt
    17041 gatgcaggat gacttgatgt ataacacgaa agcgggattg atgggggcac tagcaggaat
    17101 cggagatgca atcgattcag gaaccgtcca atatattttt atcgcaatcg cggtaccttg
    17161 ggcacaacag ggaagtcctt taggcgcact ttttcctttt atctgctttg ggttgtatca
    17221 ggttttacta ggggtatttt tcgcaagaca agctttcact atgggccgta atgctacagg
    17281 cctaaagcaa aacactggtg ttcagacagc gattgaaatg ctatcagttt tagggttgtt
    17341 tatgatgggg gtgcttgcgg gaaactatgt aaaagtagaa tcttcattga aatttgcgat
    17401 atccggacgt gagtttgtta tccaggaaat tcttgatcaa atcgtgccag ggatattacc
    17461 tttagctgtc gtaatgggtg tgtacttgtt ctatacgaaa aaaggattga aagtcacaca
    17521 agcattatta tggctgactg gaatattaat tgtgctagca ggaatcggta ttttataaaa
    17581 agaagggaac gtgaataaga tgggtgtagt caatttagcg cgtgtggatg agcgtttgat
    17641 ccatgggcaa gtaatggtaa cgttatctca aaaaagtgga gtgaattcca tttttgttgt
    17701 agacgaagtt gtagcaaaag ataaatttat gagagatctt tacaaaaatg caggaagccg
    17761 tacaggacaa aaaacaattg tcattacacc agagaaagct aaattctatt gggatgaata
    17821 tcaatttaaa gaatacaatt gtattttgat cgcaaaaaca gtccaaacga tttacgactt
    17881 agtaaaacac ggaatcccca tggaagaatt gaatattgga gggattgctc aaaaaaatcc
    17941 tgatacagat cttttagtga cgaaatctgt ttatttaaat aaatcagatg ctgaaaaatt
    18001 gaaagaaatg aatgaacaat atggcgtaaa gaatatttat tttcaagcca ctccatctgc
    18061 acctaaaaca ggattaaaag atgtattgaa caagtttgat ttgtcataaa caaatgctta
    18121 gaaaaaagat aaagagggcg aaaaaatgaa gaagaacgag ctgtttcgtg attgggaatt
    18181 tcgttatcgc tatatttacc gtaagcgccg tacaaaaaag agtaaacagc gttttctttc
    18241 agcattagta tcagatattt acagtatgag aacagatgtg acagtcattg cttacgatac
    18301 gctagcctac cgatcaaaaa atatttatgt tggagatatc gaaaaagcag agaaagttat
    18361 ctgtacttat tacgatacac ccgttcatgc attaggaagt tatttcatgt ttgattggaa
    18421 agaccaaagg aaaaaaacaa tctactctat attattgtcc ttcattttgt tgttcagttt
    18481 aggttggtgg ggaatgatga tatataacaa aaatcctcat cacgtattcg atctgctatc
    18541 cgtataaaca ggtatcactg tactagcatt tgggagctac tttttcttat tgggaaaagc
    18601 agctcgcgga tggcctagca gacaaacttt tattcgaaat acttcatcca tattaacgat
    18661 gctagaaatg atccgtacga ttgatgatcc gagtgtagcg tatgcgtttg ttgatgaagg
    18721 ctgttatgga gaaaagggct tagacagcgt gagatcgggt atgaaaaaag aggctattct
    18781 tttttatcta gactctgttg gggcagatac accgctacag ttttcgggaa attatttttc
    18841 aaataaagaa cagtggctga aacaagttga taagttgaaa gaaaaaaacg tcaattatat
    18901 ttttagtgca agaaaaaagc aagcgcagtt cttctatttg accaaaacag atttacgagg
    18961 gaaaacattt aattggcaaa atgccaatca gattattgct ctttttcgat aagtaaaaag
    19021 tgcaaaacaa ggagtgagaa gactaatgtt ggaaatcatg atcgttacac atggtaagtt
    19081 gagcgaagga ctgaaagatg cggcggaagt aattgtcggg acatcgagcc atattgaaac
    19141 aatgtgctta gagtctgggg aggacatcca aacattcgga gagaaggtca aagaaaaatt
    19201 aactatgatc aacacagcag aacccatgct agtatttgtt gatctaatta gcgcaagtcc
    19261 gtacaatcaa gcgctcttgg cagtcaatga actgactgaa gaaaagaaag cagccactta
    19321 tgtcataggg ggcgtgaatc tgccaatgtt gttagaagca atcaatcatc agatgcttca
    19381 cacaccgata gaagaagcgg tggaacaaat tgtcaaacaa ggaaaagata atttaactgt
    19441 ttggcatgct tcagcggaag aaaaaaaatt agatgaggac gatttttaga aggaaggctg
    19501 aaaagatgaa gtggggattt agatggtatg gtgaaaaagg ggattcgatc ccattaaaat
    19561 atgttcgcca aataccggga ataaatggga taattggaac tttgctgact aaaatgcctg
    19621 gagatgtttg ggagatttca gaaattgagg agttgaaagc atctgttgag agccaaggac
    19681 tggaactgct tggaattgaa agtgtagcga tccacgatgc aatcaaggca gggacagatg
    19741 aacgagatta ctatattgat aactatatac gaacaatcaa aaacttgtct cgatgtgggg
    19801 ttcgattgat ttgttatagc ttcaaaccaa tttttggatg ggcgaaaact gatttgttta
    19861 atgaaaacaa cgatggaagc tattctcttt tatacgatca gaaaatcgtc gatcagatgg
    19921 agccaggaga aatgtatcaa ttgatacaca gccagtcaaa gggattcaaa ttgccgggtt
    19981 gggaagaaga acggttgaaa aaattcgctc agctggaaaa actgtatgaa ggaatgaccg
    20041 aagaagaatt atttgaaaat ctgacttatt ttttagaaaa agtgattcca gtgtgcgaaa
    20101 aagcagatgt aaaaatggct atccatccag atgatccgcc atgggaactt tttggttttc
    20161 ctagagtgac gaaaaatctt ggcgacttga agaaaattct aaatgcagta gattctccat
    20221 ataatggact aacgctgtgc acaggatctt taggggcaaa tccggaaaac gatttagttg
    20281 aaatcatcca tgaggtaggt tcgcgcatcc attttgtcca cttcagaaac gtggagtttt
    20341 taggagaaag aaagttcaaa gaaacggcac atccctctac agaaggctcc cttgatatgt
    20401 atcatattat gaaagcgttg gtgcaagaag ggttcgacgg tcctatacgc cctgatcatg
    20461 gtagaaccgt gtggggtgaa gtggctatgc ctggatatgg cctgtatgat cgtgcaatgg
    20521 gactcactta catgcaaggt ctatatgagg cgataagtaa ggaaaaacca ctgggagagc
    20581 attatgaata aaatcaagct cttcatcttt gatatggatg gccttttatt agaaactgga
    20641 cgtttagctt atcgtgctta tgtaaaatca gcacaaaagt atgattatga aatgcgtaag
    20701 gaagtctatt atttactaac tggccaaaca gaaatggcga taagagaaca aatgggcatt
    20761 ttgtacggag aagatgttcc atatatcaaa tggcgagaag caatcaatca atataaagaa
    20821 aaaatcgtga aagaagataa gagagtatat acgaaaaaag gagcagaaga aattttatct
    20881 tttgcaaaag aaagaggaat ccatacaatc gtggcttcgt ctaatacacg ggtaaaagta
    20941 gaaatgtatt taaaaatgga aaatctatat gccttgtttg atcaaatcat ttcaggggat
    21001 gatgtaaaaa aaggaaagcc ggaaccagag atctttttga aagcttgttc aaaaatgaat
    21061 attcctccaa gtgaagcact tgtatttgaa gactcgattg cagggatcga agcagcaagg
    21121 cgagcgggca ttttgtcttt tctcatcaaa gatcatttga tagggttgcc cgatcataat
    21181 gggaagcata agttgaaagt agatgtaagt ttatatgaat ccaataaagc agactatgaa
    21241 ttttatgacc tgcatcaagc aaaacgattt ttagaaagta aagggttgta tctctaataa
    21301 agaggactat tttctagtcc tctttattta ttgtcctgca cgactgaggg aacagatgaa
    21361 aaagaagtaa ttttttattt tgaagtatct accttatttg tagtgaacaa tttagaacaa
    21421 acacaaaacg catctactgt tatctaccag tagatgcgtt taaagagaag aacgagaatc
    21481 gtccatatga gggtttcc
//
LOCUS       DACEPD010000049        21210 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.050, whole genome shotgun sequence.
ACCESSION   DACEPD010000049 DACEPD010000000
VERSION     DACEPD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.050"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IW797_001692"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="IW797_001692"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ1224475.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IW797_001693"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="IW797_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ1224476.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IW797_001694"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="IW797_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ1224477.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5848)
                     /gene="hflB"
                     /locus_tag="IW797_001695"
     CDS             complement(3743..5848)
                     /gene="hflB"
                     /locus_tag="IW797_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ1224478.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGARI
                     PAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETAKK
                     NAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATNRS
                     DVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGFAG
                     ADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYHEA
                     GHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGGRT
                     AEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQTK
                     AYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKSLF
                     EHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEENKA
                     EHDSDSQQSEDNHSDDQYK"
     gene            complement(5938..6483)
                     /gene="hpt"
                     /locus_tag="IW797_001696"
     CDS             complement(5938..6483)
                     /gene="hpt"
                     /locus_tag="IW797_001696"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ1224479.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6483..7928)
                     /gene="tilS"
                     /locus_tag="IW797_001697"
     CDS             complement(6483..7928)
                     /gene="tilS"
                     /locus_tag="IW797_001697"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ1224480.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8048..8518)
                     /locus_tag="IW797_001698"
     CDS             complement(8048..8518)
                     /locus_tag="IW797_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ1224481.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8564..8989)
                     /locus_tag="IW797_001699"
     CDS             complement(8564..8989)
                     /locus_tag="IW797_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ1224482.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9056..9325)
                     /locus_tag="IW797_001700"
     CDS             complement(9056..9325)
                     /locus_tag="IW797_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ1224483.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9336..10931)
                     /locus_tag="IW797_001701"
     CDS             complement(9336..10931)
                     /locus_tag="IW797_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1224484.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10946..14467)
                     /gene="mfd"
                     /locus_tag="IW797_001702"
     CDS             complement(10946..14467)
                     /gene="mfd"
                     /locus_tag="IW797_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ1224485.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14484..15044)
                     /locus_tag="IW797_001703"
     CDS             complement(14484..15044)
                     /locus_tag="IW797_001703"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ1224486.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15397..16371
                     /locus_tag="IW797_001704"
     CDS             15397..16371
                     /locus_tag="IW797_001704"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ1224487.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16541..17941
                     /locus_tag="IW797_001705"
     CDS             16541..17941
                     /locus_tag="IW797_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ1224488.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18019..18741)
                     /locus_tag="IW797_001706"
     CDS             complement(18019..18741)
                     /locus_tag="IW797_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ1224489.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18820..19326)
                     /locus_tag="IW797_001707"
     CDS             complement(18820..19326)
                     /locus_tag="IW797_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1224490.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19449..>21210
                     /locus_tag="IW797_001708"
     CDS             19449..>21210
                     /locus_tag="IW797_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ1224491.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTED"
BASE COUNT         5891 a         4652 c         3827 g         6840 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc cgccgccttg ttgtcccatc atcatgtaaa
     5401 agaaaaagat gatgattacg attggcaaga agctaagcaa tagggaaacc catataccgc
     5461 tgcttgattc ttcttttact gttgtagcaa ctttgttttc ttttgccagt tctgttactt
     5521 ggctcaatgt tgtatcattt ggcaaaatga ttgttgtgaa atgggtagta gtcgtatctg
     5581 ttgaacctaa gatagctaac ccaccgctgt tagcgacggt ctgctgttct ttgtattcac
     5641 cagtgatctt atatacacca tttgcaggct gcacttcaaa ttctttgatc ttgcctgctt
     5701 gtagttgttc tgtgaatttt gagtactcga tatctgacga tcgctggccg ttattaccaa
     5761 aaatgaaata aacgaccatt accatcgcta agattacgag gacataatac aaggcatttt
     5821 tcatcatgcc gttgttcttt ttgttcatag ttgccctccc taagcgtcaa tttaatttaa
     5881 cagtttttta tcaatcctga ccattctatt gtaccataat ttaattgaga tacagcatta
     5941 atttgattga taaacactag gtttcaatac accgatgtac ggaaggttac gatatgcttc
     6001 tgcataatcc aagccataac cgaccacaaa ttcatttggt acgttgaagc caacataatc
     6061 tgcttcgata tttactactc ttccttcagg tttgtcaagt agtgtaacaa ttttgactga
     6121 ttttgctttg cggtatttga aaagatccac taaataagca agtgttcttc cgctgtcaat
     6181 gatatcttca acgatcaaca gatcgcgacc ttctacattc gtatctaagt ctttgacgat
     6241 ttttacttcc cctgacgaga ctgttgcatt cccgtagctt gaaacatcca tgaaatccaa
     6301 ttccaaatac gtatcgatag aacgaacgat atctgccatg aatggtaccg ctccttttaa
     6361 gacacctact actaggggat tggtgttttg gtaaatttct gttaactctt tgcctagttc
     6421 tttgcaacga acttgtatct cttcttgtga gatcaaaatt ctctcaatat ccttttccaa
     6481 catcaggttc tccttccaat tgcttctttt tgatatttgt aaagaagtat gtagtgtatt
     6541 ttatcagttt cttcacgaat actcaaatat gaaaaggcaa aaggtatcaa gctatatata
     6601 tttcttttgg catctgtaat gatccaagca tgctgccttt gtgaattagg tactttctga
     6661 tcgataaaat atctagatac tttttttgtg agattctctg ttaatttaat gcggtctcct
     6721 gcttttcttt tttcgacata cagttgcaca tcagcaggaa tccaaatgtc atgagaaaat
     6781 agagaccatg aatctgttgc tttgatgtcc cctgcatttt caggtttgac cagaccaatc
     6841 cattctgttt cattcaaata tattccttgt tctggaatca gcaacaagcg ctggtcttct
     6901 gtctggctag ttgcttgttt ttctttttta gagagtacaa caaaatgata ctctcttttc
     6961 agtatccaat tattgccaat actgagttgc cattggcttt ttttctgttg ccactggtct
     7021 aaaatcgact ccatttgttg ttctttcaaa ataattcctg tttttgagaa tgctttgtcg
     7081 aaaaaggagt aaaaaaaata gtatcgttca gcatctgtca tttcttcaat catttctgca
     7141 gaaaatcgaa attgttcctc ccactgttcg acttcctttt cgatcaactg tcccatatat
     7201 ttttgaatga cttggtctgc ccattggatc tgttgcgaaa attgctggaa atgtctcaac
     7261 gcctgtgtat tttcttgttt caattgagga actaccaaat gacgaagccg atttctctgg
     7321 atatctaaca attggtttgt ctgatcctca aagtaaacaa gctgcgattc cgctgcataa
     7381 gtatataggt cttctttgct aaaactcagc aacggacgaa tcagtctgcc cgtagcaaat
     7441 ggttgtactt ctttgattcc agaaaaagta tttaactgtc ccccacgaac gattttcatt
     7501 aacaccgtct cgatttgatc atctccgtga tgagcagtca tcaacgtgtc atattgtcct
     7561 tcttccatca cgcgagtgaa ttcttgataa cgaaagttac gagcttctgt ttctatggcg
     7621 gatttggctg gatcttccca aatacttaca tataaaggga gattcttctc ttcacaatat
     7681 gagcgaagat aggcagcttc ttctgctgat tcttctctta attggtgatt gatgtggacg
     7741 atacctaaag aaaaaccgat ttggcgttgc acttgtaaaa gtaaatggag caaaaccatc
     7801 gaatcaacac cacctgatac cgctgctaaa attctcgatc cttgttgcca gaatccttgt
     7861 tcattcccgt gccgaataaa tgactgctgt aatagcggtg cttttttccc gcctttttca
     7921 acgttcatcc tttccacacc ctttcttcct aaaaaaaaga gagcgggaaa acgtctaaaa
     7981 cgacaattaa cctgccctct gttgatacta tgacccaaat agtcgcacta tcattatttg
     8041 cggcttttta gctgcgacgt ccgccacggc caccgcgctt tccttctgtg tttcgcttca
     8101 atgaagataa gcggtcatcg ctatctttca aaaatgaact catcaatgaa tcgaagtctt
     8161 ctttttgagc tgaaggttga ctgcgagaaa aagatttttt ctgtgctgga cgatttcggt
     8221 tgtcacgggg atctttttgt tgatattttt taggacggct ttcttctcca gattcagcag
     8281 gttgttcctg cgctttccgg atagatagtc cgatttttcc atcatcattt actgaaataa
     8341 ctttaactgt tacttcatct cctacactta aaacatcatg gatgtctttt acatagccat
     8401 ttgacacttc gctaatatga actagacccg tttttccagc acctagatca ataaacgcac
     8461 caaaatttgt gattcctgat acttttcctt gcagttttgc tcctacctcg attgacataa
     8521 aaaaaatgtt cctcctaatt atttcccttt tttatattcc tgcttatttt gcagttgtag
     8581 aactagttga ttgatctgtt tcatttgtcg agctgtctgt atttgattct aatataggat
     8641 aaactttttc accgtctttt gagtaaaaga atttgcttct ggcaagttta gctacatact
     8701 catcatcttt cagcaatgaa acatcttggt gcagctgatc aacgtctgcg ttgacttctt
     8761 tcatttcagt atttgcttcg actctcaatt cgttcaattg atgcatcctt tgcatgtcat
     8821 ggaaaatctg cacacctatt atcgcgaaaa tgaccaaagc accaacaaaa attgcagcca
     8881 gtctgcgtct tcgaaaaatc agttgacgtt gttgttgctg aaattcagca tactttttct
     8941 ttgcatattc cgtatctagc gcagcgattt tattgctttt tttcgtcact tggattcgct
     9001 ccaatctttt tgattgtctt tttcattata ccaatatcgt gaaagcttgt ctaatctatt
     9061 tctctagtgt ttctgtgcga ttttcagaaa taatttcgta cattttttgt gcatcttctt
     9121 ttttggttga ttcatgtaat tccgtaattt tcacttccaa cgttttgttg ccaaactgga
     9181 ctttcaaaat atcgcctact ttgacatcac tggaagattt cgccaattta ccgttgattt
     9241 gaattcttcc tttatcagct acttcttttg ctactgatcg tcttttgatg attcgggaaa
     9301 tttttaaaaa tttatctaat cgcatatctt attcctcact ttttaatttt tttatttaat
     9361 cgcaggattt tcttaccaaa aggcaacatt agccattctc gtactgtaaa cagacgaaga
     9421 cgaatcactg cataaatgaa cacagaagcc ccgatcccaa ctcctatcac gctgacaaaa
     9481 aaagcagtgg aacgatgagt tacaggacca aatactatat tcaatacacc ataatacagc
     9541 agcagtacta ctgtcataca aagaaggctg aacatcaact tccgtccaaa atgccgttct
     9601 ttccaaaaag catttagctc ttttgtttca attcgaacaa aataccaaag cgtcacacct
     9661 aatcctaaaa gggtagacag gcttgcacct gctgttccta ataaaccggt cagaaaagaa
     9721 gtagtcaatc cttttaccaa tagtccccat cctgcgcctt ttaaagaagg gcgaaacgag
     9781 ttcttgcttt gagcaatact ttgataagct tggatgactg cagtgaaagc aatggaaaaa
     9841 acaaataata caagcgctgc atttcccgca taatctttaa acaacgcata attgatataa
     9901 gggagcagca aggctaatcc caaagacgca gctaatgcta aagcggtcgt caatctaaga
     9961 tagattttgg ctgtctgtaa aaactgacga tagatccggt tggtcaaatg acgagtcaac
    10021 gcaggcagga atgtcgcact taatgcagtg gcaatcacta aacctaattg tactaaaggc
    10081 tgacctcgat catagacccc ttttgctatc ttcgcaccgt attctgacag tccgtaaact
    10141 tccaaagcat tttttacaaa gaaagagtcg atcagctgga aaaagataag caatccacta
    10201 taaatcgaaa ccagtccgcc ttctaccaga aaacgccgga tcaagggctt tgacggctgt
    10261 ttgaacaatg gaaaccgtcg gaagcttaac gctcctccat gaatcttttg ttcatagtag
    10321 tgaaggatcc catatgcgca taaaccgcca aaaacagctc cactcattgc tgccgtccct
    10381 gtttggtaca ccgtccatcc aagctttcta aaagcaaacg cactacagac aatcacaaaa
    10441 acacgaacga actgctcaac tacttgagaa acagcagttg gttccatcaa gaatcttcct
    10501 tggaaattcc ctcgataaaa agatagacca ggcatcagca aaaaggtaaa ggaaactact
    10561 tggatcagcg gtttcaattg tatgtctccc atcatccaag caatccagct gctaaatgca
    10621 aagacaaaca accacaaaaa gacacctgtc caaaatacta agggataaag attttgcaaa
    10681 gcttgctttc gctcgaaagg attctttctt tctgcaacgt actttgatat gaactgcgga
    10741 agaccggata aagctaaagt cattgctatc ccataaattg gataaacttg ctgataaaca
    10801 taaaaacctt cgtcgccgac taagttttgg aggggaattc ggtaaagggc acttaatact
    10861 tttgctatga aagaagcaat cgtcaataca aatgctcctt gcatgacgcg ccgcatttct
    10921 ttatgatcca aatcattgct ccttcctacg ctgacagata tttttgctca cgcaacgctt
    10981 tgacaaactt ttggatttcc tgaagccata ctgcttcttc catttggttg gggatcgtca
    11041 agcgtatcac catctgttct ttttcaacgc ctagactggc ttttagtttt gtcgctgtca
    11101 gtgcttcgaa taactgttcc acactatagc gttttgttcc aactttactt agagtaaaga
    11161 ctatatgcgc ctcctgtttc cttatcgttt ctaggagcgc tcgatcgcca tccattttga
    11221 tctgcccaat tgtcaagaga tgtgctactt cttctggata ttctccaaaa cggtcgagca
    11281 gatcatcttc tagttcatct agcatttctt gcgaatcaag ttcacgaatt cgtttataga
    11341 tctcgatttt ctgccgttga tctgccacgt atgtttcagg tagatacgca tcgacaccga
    11401 ggtcgatctc aacagtggtc ttctccactt gactattctt tccttgttta cgagaaacag
    11461 cttcgcttag catttgagaa tacatatcga aaccgactgc atcaataaat ccatgctgct
    11521 gagcacctaa gagattacct gcaccgcgaa tggaaagatc ccgcatcgca atcttgaatc
    11581 cagatcccag ttctgtaaaa tctttgattg cctgaagacg tttttcactg acttcgttaa
    11641 ggatcttctg ctgctcatac ataaagtaag catatgcgac acggctgctt cttcctaccc
    11701 gccctcgcaa ttggtataac gtggataatc ccatataatc agcgttctca acgaacaacg
    11761 tatttgcgtt cgggatatct actcctgttt caatgatggt tgtagtgaca agaacatcat
    11821 attgaccttc aataaattca aaaagcgtat tttctaattg cgcctctgtc atttgcccat
    11881 gagcataacc gatccgtgct tccggaacga gttcttggat ttcttctact ttttgttcga
    11941 tcgtttctac ccgattatat aaatagaata cttgtccacc tcgtcccatt tctcgatgga
    12001 ttgcttcgcg aatagcccca ggattttttt ccataacata cgtttggatc ggataacgat
    12061 tcgcaggcgg tgtttcgatt actgaaagat cccgcacgcc taacatcgac atatgcaaag
    12121 ttctcggaat cggtgttgca gtcagagtca aaacatccac ttgtgcacgt aactgtttca
    12181 atcgttcctt atgttttaca ccaaaacgtt gttcttcgtc aatgatcagt agacccaaat
    12241 cgctaaattc aatatctttt gacaaaattc ggtgggttcc tacaacaata tcaacttgac
    12301 ctgtacgcag ttgttcgatg gtttcttttt gttgctttct tgttctgaaa cgacttaata
    12361 gcccgatatt tacggggaaa ccttcaaatc tttccagcat cgtttcatag tgctgctgtg
    12421 ccaagatcgt tgttggaact aaaaaggcta cttgtttgct ttcctttatc gctttaaagg
    12481 ccgctcgaag cgctacttct gttttcccat aaccaacatc acctacgagt agtcgatcca
    12541 ttggtttttc tttttccata tcccgtttga tctccgctgc acttctcaac tggtcatcag
    12601 tttcagagta ggggaaggca ttttcgaatt ctttttggta ggcgtcatca ggtccaaaag
    12661 catatccttt ttctgactct cttgcggcat ataatttgat tagatcgtca gcgatatcct
    12721 cgatttttga agagactttt cgcttggttt tcgtccattc acttccgcct agcttattga
    12781 tgcgaggcgc tttggcttca gaggcgacat atttttggat cagattcagt tgagtaacag
    12841 gaataaacag tttgtcgtcg ttttgataca aaatagtcat atagtcttgg tgaacgccat
    12901 ctacttctag cgtttccatc ccaatgtatt tcccgatacc atgattggca tggacaacgt
    12961 aatctcctgc ttttaactca ttatagcttt tcaatcgctc tgcgtttgtt actgtttgac
    13021 gtctcgtttg ttttttcgtc gttttttgga aaatttcttt ttctgtgata gctactattt
    13081 tttcttgagg aagttcaaaa ccagcctgca aagctccttc aactaattgt acctgtcctc
    13141 tgatcaatgc atttttttct gtttctacta ctaatatgtc ttcatctcga agcatttgtt
    13201 ctgctttgcg tattctgtcc tttgtcggaa taaagatcac aacagtctgt ttttgcttgc
    13261 gccaacggtc catttctgtt ttcagcaatg gcatttgtcc gaagaactgt tgcatcgtcc
    13321 gatattgaaa attatggatt gcttggaaac gaagatttcc catccctttt tggaagagtg
    13381 agaaaaaggt tgtggtgaac ttttccttct gcacgatttt ttgtatatct gctgaaaatg
    13441 tctgctcaga aaacacccgc aattcgctga ttttttgagt ctgccattct gccgcttccc
    13501 tttcgatttc ccgatttgtt tccatgattc gctgataatc atcaacgact agtaaactat
    13561 ttgaagggaa ataatccaag atcgtcacag attcttggta aagaagatct gagtagaatt
    13621 ttgcttcttc tgtcgggaca cctgcttgcc agcttgaagt cagctgaccg aagtaatctt
    13681 gcaaaaactc gcggtctgct tcttctgtgg tcgcagcaag ccgtttttct agcatttttt
    13741 gcatttgctc tattccatgt tctagatcct ctggtccgta gacttgttca ctcgttgggg
    13801 aaacccagat ttcttccgtt ttttcgattg agcgttgtgt ttctgcttca aaataccgca
    13861 tagaatctat ctctacatca aatagttcta cacgaacagg ataggttgtc gttaacgggt
    13921 aaatatcgat aatgcttcct cgaatactga attctcccgg ttttgcgacc atctcttctc
    13981 gtacatagcc catcaaagtc aattggcgag gaagattttc cacgtctatc tcatcaccaa
    14041 tatgccaatt caattgcgcc tgtagccaag ttttcttatt cggcagtcgt ttgtgcaaag
    14101 atgcgactgg caacaaatag atacctgctt ttttttcttg aagtgcgtta agcgttgcaa
    14161 ctctctcgcc tcgcgcttct ggagaagaaa aggccatttc agcagaaagc acttcatcca
    14221 ctggaaataa atggatctga tcttgcggaa tcacatgttg cagatcttct gctaactgat
    14281 tcgcataata caaatttggc atcagaataa tcatgttttt atttgttttc ttaaacgctc
    14341 gagcaaatag aagtgttttc gctgagcccg ctaatcctgt gattagttgt ctaggcattt
    14401 tttcttctaa attgctgacc cattctttta cttgcggatc ttttccgact aattgaataa
    14461 tatccattcc ttttcctcct tcctcaattg aatcgattca tcgcatccac gaacgaatgc
    14521 ccttctatga gatattcgac tgctttaaca gattgacaca tgctttcttc tatcaaaggc
    14581 tgattttctt tgctgaaagg actaagcaca tgctggacta ctgttttttt cccttctggc
    14641 ctgccgattc ccaccttgat tcgatcaaag acattcgtat tcaaatggga aataatactt
    14701 ttgataccgt tatgcccgcc ggcacttccc ttttgacgga gccggatttt tcccactgcc
    14761 agatccaaat catcgtagat tacgactaat tcctctggat aaatcccaaa ataagtcatt
    14821 agtgggccga ctgcacgacc agattcattc atgaatgttt gtggtttgac taaaaggatc
    14881 ttttctccat tatgaaaaaa ttcggcaact tctgcttcga acggattttt tttgaaagta
    14941 gcttgatgct cgcgtgcaat tttatccacg accataaagc cgatattgtg ttttgtatat
    15001 tggtatttgg tacctgggtt tcctaacccg acgatcattt tcatttttgt tttccaccta
    15061 attctaaaaa gcagcacaaa aggctggact gctaattagc ggtccagtcc atcgagcttt
    15121 tttctttttt atacttgtca atgcatagta taacacaaaa caaggcgttt cggctaaaag
    15181 agaatcgtta ataaatgata aagaaattaa aaatttcttc aaagtttaat gaaataattg
    15241 atatataaaa atagtgaaat aacgcacatg taagctttta catataatac aaccttgtta
    15301 tctgttatat tttatagcca aatagcgtga catggggctt gagaaatgtt agcataagca
    15361 tgggaaaaaa caaatcaatg gaaggattga tactaaatga ctgcagctgc agaaaaaaaa
    15421 gatcatcaaa aagttattct tgttggagac ggcgccgtag gttctagcta tgcctttgct
    15481 ctagttactc aaaatatcgc tcaagaagta ggtattgtgg atatcaatac tgctaaaact
    15541 gaaggagacg caattgactt gtctcacgct ttggcattta cttcacctaa aaaaatctat
    15601 tctgctactt acgcagacgc acatgatgct gacttagttg ttattacagc tggcgcacca
    15661 caaaaaccag gtgaaactcg tttagactta gtaaacaaaa acctaaaaat caaccgcgat
    15721 gttgtcacac aaatcgttga atcaggattt aacggtatct ttttagttgc cgctaaccca
    15781 gtagatattt tgacttactc aacttggaaa ttctcaggtt tccctaaaga acgcgtaatc
    15841 ggttcaggta cctctcttga ctcagctcgt ttccgtcaag cacttgcgga attagttgac
    15901 gttgatgcac gtaacgtcca tgcttacatc ttaggtgaac acggagattc agaattccca
    15961 gtttggtcac atgcaaatgt tgccggcttg caaatctatg aatgggtaaa aaacaaccca
    16021 gaaatcgatg aagaagctat ggttaaccta ttctttggcg tacgtgacgc tgcttacaca
    16081 attatcgaga aaaaaggcgc tactttctat ggtatcgcag tagcattagc acgtatcact
    16141 cgtgccatct tagatgatga aaatgctgtt cttcctttat cagtattcat gaatggcgaa
    16201 tatggattga acgacatcta cattggtgca cctgctgtta tcaaccgtca aggtatccaa
    16261 aaagtaatcg aaattccttt aagtgacagc gagcaagacc gtatggctgc ttctgctaag
    16321 caattaaaag aaattctaga tgaagctttt gctaaactag acgctgaata atctttgaac
    16381 atgacggaaa acatctattc ggtgttttcc gtttttttat tttatcaccc ttatggtttc
    16441 taaatttttt cataaagtag agataacctt cgtcttttcc tctaaattat gctatcatga
    16501 gaccgttcca ccctaaactt ttacttaagg agttgtcgat atgacaagat cttcgcatcg
    16561 aaaaaaatcg aaacatgttg ctttgctttc agttcttggt gttctcttag tgctgatcag
    16621 cttttatacc tatcgcagta catactacac aaagcatttt ttgccgaata cagaaataaa
    16681 tggaataaat gtcagcaatc taacagtcga aaaagcaaat gaaaaattaa aagaagctta
    16741 ttctgataaa aaactggcta ttaaagaaga tggaaaagtc tggcaggaag taccaaaatc
    16801 cgagctcggc tacaaagatg atttcacttc ggagctttct ccgatcctaa atgaacaaaa
    16861 cggatggact tggggaatga cttatgtctc agcggcagaa aaacaagaaa tagatcctgt
    16921 cagtcaaaac caccaaaaac tagacactac cgttcagaca ttgacaacta agctgactga
    16981 tttgaacaaa gaccggacac ctactactga tgcaacgatc gaaaaagcag gtgactcttt
    17041 cgttatcaag cctgaagtca atggaaatac gatcgacgtc gatgctgcgg tcaaacaatt
    17101 aaaaagtgcg gtaaactctg gtaaagatac aattgaattg actgaattca aagaaaaacc
    17161 aaaagtaaca tctgaagaca gctcactgaa agaacaactc gcatccatga atgctatcgc
    17221 aaatgtaaaa gctacttaca gtataaacgg tgagactttc cagatcccat caagtgatat
    17281 tatgagttgg ctgacctaca atgatggaaa agtcgattta gatacagaac aagtacgtca
    17341 atatgtaaca gatttaggca caaagtataa cacaagtacg aatgacacaa aatttaaaag
    17401 tacaaaaaga ggagaagtta cggtcccagt tggcacttac agttggacga tccaaacaga
    17461 cagtgagaca gaagcattga aaaaagcgat tctggcaggc caagatttta ctcgttcacc
    17521 cattgtgcaa ggtggcacaa ccgctgatca tccattgatc gaagatacgt atatcgaagt
    17581 tgatctggag aaccagcata tgtggtatta caaagacgga aaagtggccc ttgaaacaga
    17641 tatcgtttcc ggaaagccaa ctacacctac acctgctgga gtcttctatg tttggaataa
    17701 agaagaagat gcaactttaa aaggaacaaa tgacgatgga acaccttacg agagtccggt
    17761 aaactactgg atgccgattg actggacagg tgttggtatc cacgattcag actggcaacc
    17821 tgaatacggt ggagacttat ggaaaacccg cggttcacac ggctgcatca acaccccacc
    17881 aagtgtaatg aaggaattat tcgggatggt cgaaaaagga acgcctgtat tggtcttcta
    17941 acaaaaataa tatataaaaa gaaagcaccc gtacgattgg tacaggtgct ttctttttat
    18001 tatctaacaa tgtgctactt acatttcagc agcagtaaag acttctgaaa catcatcatc
    18061 gtcttctagc ttatctacta gacgttccaa ttgctctttt tgttcctcat ttaaatcaat
    18121 cgtcgtttgt ggaatcatcg tcaattctgc ttgcgccaaa gtgaactctt tttccaactc
    18181 atctcgtaca gcagcaaaat cttctggtgc agtataaatc tcgaatactt caggagaagt
    18241 ttgcagatct tctgcccccg cctctaatac atcctcaaac atcgtctctt catcaacagt
    18301 caacccatcg cgttcaatag cgatatatcc tttgcggtca aacaagtaac tgactgaacc
    18361 cgtttctccc agtgatccac cgtttcgagt aaaagctact cggacatttg tcgctgtccg
    18421 attacggtta tctgtcaatg catggactaa gattcctact ccacccggac catatccttc
    18481 atacgtgatt tcatcgtaat tcgtttcatc tacggcactc gtagcctttt taatcgcccg
    18541 ttccacattg tcgttaggca tatttgcagc ttttgctttg tccacaacaa gccgcaaagc
    18601 tggattcata gatggatcag gaccgcctgc tttagctgcc atataaattt cacgagaaag
    18661 tttctgaaag actttccctc gttttgcatc ttgggcattt tttcttcctt ggatattatg
    18721 ccatttacta tgtcctgaca tgatttttcc tcccttgttc ttttgagaat acacttctat
    18781 tctagtacta tttcacaaaa aaacaaagcc acagccaatc tataaacgct tcttgaatgc
    18841 cgcaaaaagc acacataata tcacagcagt cagagcaccg atcgaatcca acatgacatc
    18901 ttggaacata ggtgttcgtc caccggtcat catctgatgg aattcgtcca aacctgcgta
    18961 ccctgttgct gaaagccaag caaggatcgc tgtcagccac caaattttta gcttggggaa
    19021 aacacctaaa aacaagctgc ctcccaaaat aaaataggta ccgaaatgtg cgcccttcct
    19081 taaaaagaac tctaaaaatt tggcgtagcc cagatggtcg atactcacca tacttccacc
    19141 ataattaaaa cgtatacccg ctaatgtgtc tttaaatggc tgattaggca gccagctttc
    19201 taatcgcgaa acttgtgatt gctgtccgta agtttgagaa gaactgtaaa ataagattat
    19261 aacgactatc agtgcgatta ctaaataaat atttccgttt ttcatttgtt tttttgaaga
    19321 attcataagt cacctctgta ttttatctta gacttttttt gaagaaacaa caatcatttt
    19381 catagagact tcttccataa aaaagtgcta tacttaattt tgtattacaa tatgaaggga
    19441 gttttttcat gagttattca ttaaattgga atctagattc tattttttcc ggaggcagtc
    19501 attctgatgc tttgaaccaa cggatgaagc aattggaaga ccaaatgaac gagtattatc
    19561 accgcgtaac aaaatggtca cctagttctg acaatgcaga gcagttaaac gctattcttc
    19621 aattgcaaga aacgattacg aatggtttta cccaatgcag cagctatatt actgcattgc
    19681 tttctgccaa tgtcaatgat tcagatgcaa aggtcttatc aggcaagctt tacgcaatgc
    19741 tgcctcgtct gcaatcagcc gaaactgtat tgtcaaagaa atttgcagaa atatctgaca
    19801 atgattggaa tcagatgctt tctcaatctt cgtttgaaac aattgctttt cgtttaaacg
    19861 agattcgtcg tgatggctca caattattat cagaagctga agaaaacatt ataaatacgc
    19921 tttctttaga tggtctaaat gcttggagca gtcattatga tacgattgta gccagcattt
    19981 cgattccctt cgaacaagat ggacaagtag tcgaattatc tgctggacaa gccttcaata
    20041 agatgatggg cgatccagat ccaaaagtca gagaaacact tttcgcagca tgggaagaag
    20101 cctggaaaga aaaggcaccc ctcttcacgg atactttgaa tcatttagat ggttttagac
    20161 tttctgacta tgaacttcat ggtgtcacag attttctaca aaaaccatta gagtacaatc
    20221 ggttgaagaa agaaacattg tctgtcatgt gggatacaat ccagaaaaac aaacaacccc
    20281 ttgttaatta cttgactaga aaagctaatc ttttcggtaa agaaaaaatg gaatggcaag
    20341 atcaagatgc acctattatt ttaggcgatt tgaaagaaaa aacctttagt tttgacgaag
    20401 cagcagcatt tattatcgaa aactttaaca aattcagtcc aaaaatggct aaatttgccc
    20461 aatctgcttt tgaaaagagc tggatcgaag cagaagatcg tcctgggaaa cgtcctggtg
    20521 gttattgtac tgagttacca gaaacaaaag aatcaaggat ctttatgacc tacagtaatt
    20581 ctgttaatga ggttgcgaca cttgctcatg aactaggaca tgctttccat agcagcacga
    20641 tgtgggattt accttcttta aatcgcgaat atgccatgaa tgtcgcagaa acagcaagta
    20701 cattcgcaga actgatcgta gccgatgcta cattaaaagt cgcaaaaaca aaagaagaaa
    20761 aaatcaattt gctagataca aaactgcaaa atgctttagc tatgttcatg aatatccatt
    20821 cccgattcat ttttgaaaat cgattctatg aagcacggca agaaggatta gtctcagaag
    20881 ataagatcac agagatgatg gttgaggcgc aaaaagaagg ctatcaagga gctttagcta
    20941 cttaccatcc atatttctgg gctgcaaaat tgcatttctt tattgatgac gtaccgtttt
    21001 ataacttccc atacacattt ggctacctct ttagtttggg catctatgct tacgccaacc
    21061 aaaagggagc aaattttgaa gaagaatata tccatttact acgcgatact gcctcaatga
    21121 cgacggaaga tcttgcgaag aaacatttga atgtcgatct tacccaacca gatttttggc
    21181 aagcaggtat cgacatggtg actgaagata
//
LOCUS       DACEPD010000050        21008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.051, whole genome shotgun sequence.
ACCESSION   DACEPD010000050 DACEPD010000000
VERSION     DACEPD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.051"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            204..3482
                     /locus_tag="IW797_001709"
     CDS             204..3482
                     /locus_tag="IW797_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ1224492.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(4023..5930)
                     /locus_tag="IW797_001710"
     CDS             complement(4023..5930)
                     /locus_tag="IW797_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ1224493.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6060..6935
                     /locus_tag="IW797_001711"
     CDS             6060..6935
                     /locus_tag="IW797_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ1224494.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSEKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            7015..7989
                     /locus_tag="IW797_001712"
     CDS             7015..7989
                     /locus_tag="IW797_001712"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ1224495.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8178..9314
                     /locus_tag="IW797_001713"
     CDS             8178..9314
                     /locus_tag="IW797_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1224496.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9451..9795
                     /locus_tag="IW797_001714"
     CDS             9451..9795
                     /locus_tag="IW797_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ1224497.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10066..11352
                     /gene="efmA"
                     /locus_tag="IW797_001715"
     CDS             10066..11352
                     /gene="efmA"
                     /locus_tag="IW797_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ1224498.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11490..12089)
                     /locus_tag="IW797_001716"
     CDS             complement(11490..12089)
                     /locus_tag="IW797_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ1224499.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12493..13614
                     /gene="mnmA"
                     /locus_tag="IW797_001717"
     CDS             12493..13614
                     /gene="mnmA"
                     /locus_tag="IW797_001717"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ1224500.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNDKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13860..15281
                     /gene="arcD"
                     /locus_tag="IW797_001718"
     CDS             13860..15281
                     /gene="arcD"
                     /locus_tag="IW797_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ1224501.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15295..16413
                     /locus_tag="IW797_001719"
     CDS             15295..16413
                     /locus_tag="IW797_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ1224502.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16473..17765)
                     /locus_tag="IW797_001720"
     CDS             complement(16473..17765)
                     /locus_tag="IW797_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1224503.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFLVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17915..18475
                     /locus_tag="IW797_001721"
     CDS             17915..18475
                     /locus_tag="IW797_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1224504.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18604..18981
                     /locus_tag="IW797_001722"
     CDS             18604..18981
                     /locus_tag="IW797_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ1224505.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18914..20986
                     /locus_tag="IW797_001723"
     CDS             18914..20986
                     /locus_tag="IW797_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1224506.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         6806 a         3803 c         4468 g         5931 t
ORIGIN      
        1 ttgttttatg ctctttttta tacgttattt taaaaaaact tattcttttt acgttaataa
       61 atctctttat tatcaacaac ttaagttata ttttttcttt ttataaaata cacttattat
      121 aattttgttt tttattgaca gtaaaaataa attaggtaat actttaagta aacgctttct
      181 aatttgttac gggaggaata cacttgttaa aaaaacgtat aaaatacagt gcgctaatga
      241 tgaatctcct tatgctcagt accccggttt taatttctgt cgttgaagtt gctgctaacg
      301 aacaacagtc tgtgaacgaa gaaaatgcac acgtgaacat tgaagggatg tcaacaatgg
      361 aggaaatacc aagaactagt gaagatatgg atacggaaga aggaaatgat cctatgatga
      421 aagaatcaga aaatgaaaag acaaaaaagc aatctgaaga agcaaatgaa gagtggaaag
      481 gggttgtttt cggagaaagc acaagtgcta gtaggtatgc cattgaacca ttagaagacg
      541 gcgtgcggct ctcttccacg aataacggcg gaaaattcca gtcaagcgga tcagatggaa
      601 tgacctatta ttatcaagca atccccaaag acatcaactt taggatgcgg gcgacgatcg
      661 aaatagaatc ttggacctat acgaatgccc aagaaggatt tgctttaatg gtaagagacg
      721 cagtaccaaa aaatcatttg tttgggcagg cattttattc aaattcattt gccatactag
      781 gcagtcggat cgaatatgtc tgggactcgg atagacaaga agtggtcaac acgagcggca
      841 gcaaatatac gatgcgactt ggattgggga cacgggcaat cacaggaata tcatctgaag
      901 atccgcaagc tgcgccagct cctgggagtg tctcagtgga aacaacacct ttagagactt
      961 cagcagggtt cttggaaaaa gaaacaggtt cttataatat attcggaaac ggtcatggga
     1021 atctgtttcc ggccacgaac tcgattacta aactggacgt agagatgaaa aaaacgaata
     1081 ctggtttaga ggcatattat tacgatcctg aaacaggaga agaaatggct tctgacatca
     1141 tgtatgactg ggaaaaatta tacgcttcag atgaatcggt tatttatgca ggatttgctg
     1201 cagcaaggaa tatgacgatc aaggtagaag gaatcgaagt tgcttactca gatcctgcga
     1261 cagatcctcc tgggcaagaa agacctactc gcttgattga tccgatttac actgtgacct
     1321 ctagtaatca ttcaggaaac caagatcata ctatgtcctt tagagcaaat gctgacggat
     1381 tattgacaat taaaaataaa gcaacaggag aggtgcttaa gaacgcaaaa aacttggcta
     1441 tcactgcgaa tcgggaattt gattatcaga caaaactgat ggaaggaacg aacgaattta
     1501 ctttcagttt tacgccagat aaaaactatc cgccagaaga atacgtagag atgactagtt
     1561 atgaaacaaa agagattctt catacagtgg actaccgtaa acctaaatat tcgacagttt
     1621 atgtaacacc agaagggtca gatgcaggaa acggaagcag acaaaatcca ttatctctga
     1681 cacaagcatt gagatacgca tatgctgggc agacaatcgt catggcgcct ggaacttatt
     1741 ccttcgaaag aggattgacg attccaaaag gcgttaacgg gacaagagaa aacccgattc
     1801 agttgattgc agagaaaaat ccagaaaaca agcgtcctgt acttgatttt aaaggaactg
     1861 gaaatgggat gacactgttc agtcactact ggggtttacg agggtttgac attacaaata
     1921 gtgcagctat gcaaaaagga ttacagattt ctggaaatca taatttgatc gaagagattc
     1981 atgcttatcg gaatggaaat acaggaatcc aaatcagcgg tagctcaaat gatcctttcg
     2041 aagcatggcc cgcgcataac ttagtcaaga actgtacatc ttttgaaaat gccgatccag
     2101 gatttgaaga tgccgatgga tttgcggcca aactgacaat aggagaagga aatgtatttg
     2161 acggttgtat cgcgtatcat aatgcagatg acggttggga cctttttgct aaaaatgaaa
     2221 caggttcgat cggaaaagta atcatcaaaa actcagttgc ttatcgaaac ggctgggtac
     2281 caggaattga gggagaaggt aacggtaatg gattcaaaat gggcggttct tctttaacgg
     2341 gccctcatga attgatcaat agtttgtctt tcgaaaatct tgcgaaagga atcgattcca
     2401 atagcggaac agatattatc gtcaatagca gtacaagttt taataacggt tcatataatg
     2461 tggctcttta tacctcttca gcaggaaaca cagactacca tgcttctggt atcttatctt
     2521 tccgaacaga gaatcttgag atgagagagc agatccgccc attgaatcaa aacgaagatc
     2581 aaatctacca ctcgttgaac tactattgga atgaacgaga gaaacaatca gaaaatactc
     2641 ttggacatac catatcaaaa gattggatcg agtctctaaa tacatgttcg aaagaaggtc
     2701 agcagcctgt tacgcgctat gaaaatggaa gtatccatgt tcatggactg atgcagatta
     2761 aaccggggaa tcgtcaaaca gaaaaggaaa gagtggatgg tcttccatta tctgtaggtg
     2821 ctatttttga cggagaaacc tcaccgtctt cggaatatcc agagtatgca atcgtacaag
     2881 aaccaaatca gacaaaacca aatgaacaaa ataatccgat tattatacaa gaagcaaatg
     2941 gtgtggcgga gaatgcagaa ggaaatatca ttgaactgaa gccttttaca gtttttgtgt
     3001 caactttcac accaaatcaa ggaacgcctg tctttgaaga tatgcgttat ttcccagaaa
     3061 agatccaaat cgttgaaaca cagtcaagtg ctcaattttc taatcatacc gctatttttt
     3121 cagcaccttc aaaaggagaa tatacgctag aagtcactta tcgttcggaa atatttgatg
     3181 gggaaaaatg gtctgctcat acagaatctg ttacccagca aaaagcgatc actgtgcgag
     3241 aaactgccga tgtgcccccg ggaaatgaca aaccagaaag agaaaatcca ggaaatgaat
     3301 caccaaatgg aaaaccaaat ggaaaacctt ctcatccgga taggacagca aaagaaagtg
     3361 aaaaaaatgg cactcaagga ctcccaaaaa cgggagaatt taataacccg ttgctagcat
     3421 tagctggagg aattttgtta atcggtgtag ttgtttatgt catgaaacag cgaaaaaaat
     3481 aagtggaatt tctcctttta tgatgtaaaa caattgactg aggaaagtaa aaatagaagg
     3541 aaatgagacc aatagatgtc tcacttcctt ctatcttttt atagatgagg ttctccttgc
     3601 tgcttttgat tggctgttct tattacttga tgcaggacag ctttttcaca atctctccac
     3661 taaacggtgt tcaatcagct gaccctcggt attagctcaa caaacggcaa aatatgagga
     3721 actatttttc cgtttttttc tctaatactc aggtcaggac gctgatttca caacctctcg
     3781 ataaacggtg ttcaaaagcc agtttctcgg tagtaagtca aatttatgca aaaatgtgga
     3841 aagccatttt cacaaaattt ttcttactac ttgaaactaa gcagcttttt cacaacctct
     3901 aatacatggt attccagaag taacttcttc gaaaaataag ccgaaatgtc acaaaaattt
     3961 gagaagcgat tttcccaaat tccttcttat ttcttgaagc tgaccacttc tggcacaacc
     4021 tcttaccaga ttcgcactct tttttctggc gctagataca tagcgtcttg ttccgtgata
     4081 ccaaatgttt catagaattc gtcaaggttt tttggttgca cgtttgctcg gagtttagca
     4141 ggggcatgga catcgatttg cagtaaaagt tgttggtatt ctttttttgc ttttgtacgc
     4201 caaatcgttg cccaattgac aaagaattcc tcgatagaaa gatctgattc cgtttttgct
     4261 gcttccaatg cacagcttag accgcctgca tcagcaatat tttccgaaac ggttaatttt
     4321 ccgtttactt ttccgtcagc aaaaggcaga ccatcgaatt cttcgatcat tgcttgtgct
     4381 ttgttttgaa attgtgtaag atcttcttct gtccaccagt tgttcaagtt gccatattcg
     4441 tcaaataaag cgccgttatt atcaaatgca tgggaaatct catgagcgat aacagctccg
     4501 attccaccat aattagcaga gcttgattgt tctagactgt aaaatggtgc ttgaaggatc
     4561 gctgccggaa agacgatcac attccgaaat ggatgataat aagcattgac cgtgtcagca
     4621 ctcatttccc attctgaacg atccactggt ttgttccatt tgctgaaacg atcttttaat
     4681 gtgagtttgc tgaaaacaag agtatttgaa agcaaagtac caccctgatc tgcaggtact
     4741 gttttgaact ggtcgaataa tgctgggatc ttatcggggt agcctacttg gatacctaag
     4801 ttatttaatt ttatgacggc tttttctttt gttgcatccc caagccagtc attttcttct
     4861 agacgttttt tataaacggc gatcattttt ttcaccattt gttctacgtc ttttttagca
     4921 gcttcgccaa aatatttttt tccgtagtaa tcgccaacta cttgatcgaa ttgtccagag
     4981 gcaaggtaat acgctgcttt tttctgaggc attgcttcgt ctgttccaga caatacgcga
     5041 gaatagattc cgctgacttg gcggaattct tctgatagat aaccactaag agaacgtact
     5101 gttttaatga tcatccagct tttgaataac ggaaaatgag cttctgtcag gatgtcattc
     5161 aaatgatcga agtacactgg atcagttacg atcactttat ctggagttgt cccaatcaaa
     5221 cccgtgacaa gttttttcaa gtccagttta tctgtgtatt taataaatgt atcaaaagat
     5281 tgcggattat acattttgct gtaatctgcg ttttcttctg cactttttac atgaggagca
     5341 atcaatttat caaattgcac tgcttgttcc gtgattgctt ctgcctgact tttttctttg
     5401 ccggcaagct ctaataactg aacagccata tcaaagaaaa cgcctattag ttgtgcaccg
     5461 ttgggatggt ctgatgcata atacgtctta tctggaagga ataaagaagg cggataagca
     5521 aagaaggcgt tcgtttgcgc attcttcata tctgcatcta catccagatt aaatggcaat
     5581 ggtaatgtat ccaacagcca ttctggaaat tgcttattca attcgtcgaa agactgcaat
     5641 gattcaactt tttcgattat aggttgaagc ggttgagcat gcaactcatt tcttcggtca
     5701 taatcgtttg ccagttgata atatgcaagg aaatgtttca ttttttctga agacactttt
     5761 tctggatggg caagcagatc gtctgtttct cccatcaaaa gttggtcgat cccgtcaact
     5821 aaatcttgga aaccgcctgt agctggttta tctgcaggga tttttgctgt tttttgccat
     5881 tcttcattga ctgcttcata gaaatcttgt ttcaatcgtt ctttgttcat tcatcgatcg
     5941 ctcctttttt gtttcgtgtg cccattatct tacaaaatga aggaattaac aaatagaaca
     6001 taaaaaattg aaaattcgaa caatcaaact gatttgtttt tttcaatcct tcacttataa
     6061 tgaaaaaaag gaggcgatcc caactgaatc aagtagtgga taaaccttta tatttcaaag
     6121 tagataaaaa aattaaaaaa ttagccagca ctcagcagct gcagtcaaga aaaagtaaat
     6181 tgcttttttt agctctggtt tttgaagatc aaagttatgt gattatcgat caatcaggac
     6241 atccggtaga atattctcca gcaaaatata cttatcaaga gggtctttct tgtaaaaaat
     6301 ggaagctgat caatgaagag ccaatcgagt tatctagatg gatcaacaga aaagaagata
     6361 ttcccgtttt gatcgaagaa aaaaggtcag aaaaggaact cgcaaattgc tgggtgggat
     6421 tgccagaaga acgttttttg aggtataaga aatgggctac cccctcaggc tatctttgtg
     6481 gtacttacgc ggcagcagtt ctccttgcct attatcaaga ctatcgtaag gggtggatgc
     6541 ttccaaatga gattcgcaag aaaaatacgg ctgattcgtt ggttttgacg aaggcattgc
     6601 gtagccagat ccaaccacta ggacttccta cgattccttt tcaggttagt acagggattt
     6661 ctagtttttt aaagaagaat ggcaatcatg agcgtgcacg agcaactctt ctaggttcat
     6721 ggcagcgagc tacaaaacgt atacgtgaag gcaaacctgt gatgattggt attttgaaag
     6781 tacttggcag tacatatggc aatcattggg tgacagcgta cgcttatttt gaaactgaaa
     6841 caggagaacg ttattacaaa gtacacgaca actggggcga ttatcataaa gtcattcctg
     6901 ccagttggag taacggaaca gtttcacttc cttaagaatt tgctgaaatt aacagaaaat
     6961 cttggaaaaa tatgttaaac tatcgaagaa tagagaagtt ttggggagga ctacatgagt
     7021 aataagtatc aggatgacac gttgaagatt tttagtttga atggcaaccg tccattagct
     7081 gaaaaaatcg cgaaagtgtt cggaacagaa ttaggtaaaa gcgtcgtgaa gcaatttagt
     7141 gatggcgaga tttccatcaa tatcgaagaa agtattcgag gggatcatgt gtatatcgtt
     7201 caatcaacga atgcacctgt caatgattac tatatggaac tactgatcat gatcgatgca
     7261 atgaagcgag caagtgcgaa aacgatcaat gtcgtattgc cttactatgg gtatgctagg
     7321 caagaccgca cggcgaaacc tcatgaaccg atcactgcga aattgatcgc taacttgatc
     7381 gaagaagcag gggcaactcg agtactgact ttagatctgc acacggttca agtacaagga
     7441 ttctttgata ttcctgtaga taatttattt acgatgccgt tgtttgctca ttattaccgt
     7501 caattaggtc tgacgggaga tgacatcgta gttgtttcgc caaaaaacag cggtgttcag
     7561 cgtgcacgta gtctttcaga atatttgaac agcacattag ccatcgtaga tcatgcagat
     7621 gatgcttcaa ctgagggtgg ttatgtgatc ggtgatgttc aaggaaagac atgtatcatg
     7681 gtagatgata tcttgaacac aggagcaacg cttgcacgag cagcaaatgt attaaaagaa
     7741 aacggcgcaa aagaagtata tgcctgtgcg tcacatggat tattgtccga tcatgcgaaa
     7801 agtattttag acaatgcacc aatcaaagat atttgtatca ctgattctgt tttcacagaa
     7861 gaaaatcgtc atccagaaaa cttaacaatc gttacatgtt cagaattaat gggtgaagcg
     7921 gtcaaacgca tccatgaaaa tacaccgatg agtccgttat tccgtctgga agaaaaagaa
     7981 ttcgaataaa caaaaaaaag agggaaagaa gttgtgacag aagtgagcag cttcaaggag
     8041 ctgcgacaag acttttgtca cagctccttt tttctatgcc catgaaacgt cctctagaca
     8101 aaaatgagct cttctttggt ttgttcgcta ttttatgcta gaatgtgcct agcaataatt
     8161 tggaagggat gaatgaagtg aaaactattt atctggatca tgcggcaact acccccatgc
     8221 atccacaagt aattgaagcg atgacggcaa tcatgcaaga cacgtttgga aatccttcaa
     8281 gcatacatgg atttggtcgc cacgcgcatg aaaaattgga aacagcaaga caaatcattg
     8341 cagacagtct gcaagcaaaa cctcacgaga tcatctttaa cagtggggga acagaagggg
     8401 ataatacagc tatcttagaa acagcgttct cgcgtcaaaa agaaggcaga catctaatca
     8461 ctacagctat cgaacatcct gctgttctta ataccatgaa atatttagag acaaaggggt
     8521 ttgacgttac atatctgcct gtagatgaaa aagggaatct gtcgatcgaa gaggtcaaaa
     8581 aagcgttacg agaagatacg atcttggtct ctgtgatgta cgggaacaat gaaatcggaa
     8641 atttgatgcc aatcaaagaa atcggtgaac ttttagcgga tcatccagct tatttccata
     8701 cagatgcggt acaagcgtac ggaaaccaaa cgatccatcc agaagaatta ggaatcgatt
     8761 tattaagtat ctctgctcat aaaatcaatg ggccaaaagg cgtcggtttc ttatataaac
     8821 gagacggagt aaatatccct gcattgcttt taggcgggga gcaggaagaa aagcggcgtg
     8881 caggtacaga aaatttagca gggatctggg gtatggcaaa agcagttgaa ctgctgtcac
     8941 ctgaagaacg tgaagcacgt aacaacaaat ataaaggatt ccagcaattg attcttgata
     9001 cattagaagc aaacgaaatc ccttatcaag taaatggtga tttaacgaat aaactccctc
     9061 atgtgttgaa tcttcgctta attggcgaag caaatgattt gctactgatg aagcttgatc
     9121 ttaatggggt cgctatttct actggttcag cttgtacagc aggaaatatc gagccttcac
     9181 acgtattaga agcaatgtat ggagaagata caccgatact aaaagaatcc atccgtatca
     9241 gctttggttt gggaaatacc gaagacgaca tccagtattt ccttgatcaa ttagttcgtg
     9301 ctgtcaaaaa ataactagtg aagtcattct tatatgaaca gatgtctgag agaacgcctt
     9361 cattttttag ggaacttaac tgaaaaacct agtaaaaatc aggataaact tttataataa
     9421 agagaaacta attttatcga ggtgaaaatc atggcatttg aaacaacagc aacggtactg
     9481 ggcgcaccaa ttagctatcg tttgcatcct gatgcaaaaa gatatacatt gcgcgataat
     9541 ggatttacag aaacaaacgg aggcaattat caattgatcc gaccattaga tgccacgccg
     9601 caaagcaaag aaggattcaa attaaaaatc actgtatcaa aagatattca aacattgaaa
     9661 atgtcgatca ctacagcaaa tggattgaaa gcagtcaata ttttcaaaga tccaaaacag
     9721 aaaatgagcc aagataaatt ttattttctg atggatggga tgattagccg tgggttattc
     9781 gaaaaagtcg aatgatctgt ggatataaaa agacaactag ttttgaaacg gcctattgtt
     9841 agatgactaa ctttgtaagg acgacgcaaa ttagttgtct tttttatttt taaatttaac
     9901 acatgttgtt atttttaata ttttgctgat ttattgtttc taagtaaaga aatttaaatt
     9961 gaaacgattg ttatttaggg aaaaaggtat tttttgaaaa aagaatcccg tataatgatt
    10021 gttgtgcaaa cgctatacaa aatgaaaaag aaaagaggga tgtttatgga aaatgaacag
    10081 tcggtcgttt taacgaattg gaagagaaat tatttgttct ttttatcggg acagtttttg
    10141 tcaggaatca ctagtatggt cgtccaatat gcgatcatct ggtatttgac aagagaaacc
    10201 ggttcggcga caattttgag ttttgctact ttactaggaa tgattcccat ggttttacta
    10261 agcccatttg taggaccgct agtggatcgc tgggataaaa aagctctttt gattgtcacg
    10321 gatattattg tagcgatttt cgcgcttatt ttagcagtag tcggaacgat ttcagaatcc
    10381 ttcccgattt ggctagtttt tgtgtctttg tttatgcgtt cggtcgcaca aacattccaa
    10441 atgccaacga tccagtcgat tatgccgaca atcgttccat cttctcacat aacaaggacg
    10501 aatggacagt taggaatggt ccaatcagcg aatttcatca ttgcgccagc tctaggtgcg
    10561 gctttgtttt cagtcgttcc tgtcaattac cttattttat tagatgtatt gggggcagtt
    10621 tttggtgtcg gcttattgat ttttgtgaag atcccaaaag tttctcctga aatattggaa
    10681 gttccgctta ctatttttaa agatgcaaaa tttgggctgc agcaattgat ggataataaa
    10741 gggttatggt acataacgat caatggtgca tttgtgatgc tgttgttcat gccagcaatc
    10801 agtctgtatc cattgatgac gctagattac tttggtggtt ccgttggaca ggcgggagca
    10861 gtggaagtag tctacgcagt cggaatgctc ttaggcggtg cactgatcag ttttatagga
    10921 acatggaaag accgaatgaa acctattatt atagcctaca tcattatggg cctgacgatc
    10981 ggtgcaagcg gattggttcc aaacgatagt caagggttct tgtactttct tatcttgaat
    11041 gcaggagcag gttgtgcaac gccatatttc aatacactgc tgatggcaat gatccagcag
    11101 agttatgaat cgaatgtttt aggtcgtgtc ttagggaact tcaactcttt gatgaacctg
    11161 gcaggtccaa ttggattatt atttgcagga ccattagctg atcgtttagg ggtagaaaaa
    11221 atgttcttgt tttccggaat cgggatttta ttatgcggaa tcgttttatt tttaacgtca
    11281 gctgcccgaa attatgataa agaattacag aaaaaactgg taaaagaaca ccatgagcaa
    11341 aaagacgaat agtatggaat ggagttgtga cagaagtctt gttacaactc catttctgat
    11401 cctaatgttc ctgtggcaat aagcctactc aaatgaaaaa tatgagtaat tatctttatt
    11461 tgtttaatac ttgatatagg gcagtttttt cacgacctat cgtacttcct ctttgcttca
    11521 tagatttctt tgatttgttg ctcagataac ggagtgccaa atggtagttt ttcctttaag
    11581 agttctcttt ccggtgcgtc tacgcctaca ttgacatcgt tctcgatagg tacgctgtaa
    11641 tgatggatat gtccatgaag attgcggata gtgtcgttag gcccgagcaa catcggataa
    11701 tgagtcaagt aatattgttg atggtcgaat ttaacgatgg cacctacatc ataaaaataa
    11761 aatttgggac ttccgtcagt aagcctagga tcattttttt ctaaataacg gaaaaaagcg
    11821 cgactgtcat ggttcccttt gatcaagtgg attgttcctt gcagttgagt gaccagttcc
    11881 aaaatttctg catttgcttt ttcatattgc ggatgcatag caaggtcgcc tagatgataa
    11941 acatggtcgg tttcttttat tactgcattc cagctagtga tcaactgttg gttcatttca
    12001 tccacatttg caaagagccg cggagcaaag tcgttttttc ctaataagcg ttcatggaaa
    12061 aagtgcatat cactgataaa gtaattcatg tatcttttgc ctcccttttt tcttagagta
    12121 aacaaaatca cttatctgag caactcataa catctaaaaa gaaaaggttg taaatgagac
    12181 gtgcagcttt aagcgataag aagcaatttt gaaaaatagt ttttcacaat ttataaaatt
    12241 attccttatt gccgaaaggc tggccctttt tatgagtatg ccaatttttt atatcgaatg
    12301 ctcaaaagat gagtgatttt ttggttgact atcatgtttg tgtactgaat aggtccagta
    12361 gaacagtatt tttacgactt gataattaag ggatgaaatt ttcagaaata tgacgtataa
    12421 tgtaaaatac tgaaaagttg cgaccttcaa ctcgtagatg tttcagaatc taattgaaat
    12481 gatggtgata tcatgacaga caacagcaaa acacgcgttg tcgtagggat gagcggcggt
    12541 gtcgactcat ctgtaacagc acttttgtta aaggaacaag gatacgacgt gatcggaata
    12601 ttcatgaaaa attgggatga cacggacgaa aacggtgtat gcacggccac ggaagattat
    12661 aaggatgtag cgaaagtagc tgctcaaatc gggattcctt attactctgt gaattttgaa
    12721 aaagaatatt gggatcgtgt atttgaatat ttcctagccg aataccgcgc aggacgtaca
    12781 cctaacccag acgtgatgtg caacaaagaa atcaaattca aagcattctt ggattatgca
    12841 atggatcttg gcgccgatta tgtagctact ggacattatg cgcaagtcac tcgcgatgaa
    12901 aacggcgtgg tccacatgct tcgtggagta gacaatgata aagatcaaac ttacttcctt
    12961 agccaattat cacaagaaca gctttcaaaa acgatgttcc ctctaggcgg catggaaaaa
    13021 tcagaagtac gggcaattgc tgaacgcgca ggccttgcga ctgctaagaa aaaagattca
    13081 acgggaatct gctttattgg tgaaaagaac ttcaaacaat ttttgagcaa ctatctgcca
    13141 gcaaaaaaag gaaatatggt cactcttgat ggtgaagtca aaggacaaca tgctggcttg
    13201 atgtattata cgattggtca acgtcaagga ctagggatcg gtggcggagg agattcgcaa
    13261 gaaccctggt ttgttgtagg aaaagatcta gcgacaaaca cattatatgt ggggcaaggg
    13321 ttccatcatc ctgcattgta tgccacaagc ttggatgcaa gtgaaatcca ctttacgacg
    13381 aatgaaccaa tgccaaaaga attcaagtgt acagcgaaat tccgttatcg tcagcaagac
    13441 gttcctgtga cagttcgctt attggatgat aatcgtgcag aagtcgtatt cgacgaacct
    13501 gtacgtgcaa tcactcctgg acaagcagtt gtattttatg atggaatgga atgtctgggt
    13561 ggtggtttga tcgaccacgc atatcaagaa acaaaagttt tacaatacgt ttaagcgata
    13621 ttaaatgaat caaaacagct atggctaaca aaagaaacac cttttctttt ggatagcata
    13681 gctgtttttt tgcaataaaa ttaattttta ttcattttat acttgaaata cttgtgtaaa
    13741 aagttcataa tatccaataa tatgaataaa gttgcttttt gttaagaaag agcagttgca
    13801 aaatagaagg gaatcttttg caagtgattt gttcaatttt ataagtgagg gtgatggaaa
    13861 tggaaaagaa atcgacgggt attagcaaat ggggactggt tgctctggtt gtcagttcgt
    13921 caatcggaag cggtgtgttt gggatcacaa gtgatctggc tagttctgca gctccagggc
    13981 cagcgatcct ttcatggata atcgttggga ttggtatttt agctttggta ttatcgctga
    14041 atcatttagg agaaaagcgt ccagatctag atggtggtat tttcggttac gcggaagctt
    14101 catttggtaa attaggtggc ttcatcagtg gttggggtta ctggttgtct gcgtggctgg
    14161 gaaatgttgc ttttgcaacg atgctgatga gcgccatggg agaatttttc ccgatattca
    14221 aaggtggaca aaatgttcct tctattttat ttgcaagtat ttttatttgg ggtctgactt
    14281 tattagtaaa caatggagtt gaaggagcga gcgtcatcaa tacaatcgtg acgatctgta
    14341 aattagtgcc attattccta tttttaatat ttgtatttat cgcatttaaa gtgaatatat
    14401 tcactgctga tttttggggt aacgtatctg ataatctagt gaatggaaca gggcagcaag
    14461 gaacgatctg gttgcagatt aaaggttgtt tgatggttat gatgtgggtc tttgtaggga
    14521 tcgaaggcgc aagtgtttta gccaaccgcg cgaagaaacg ttcagaagcc cagcaagcat
    14581 cgatcattgg tctgctttgt ttattgttta tctatatcct tgcttcgatc ttgccatacg
    14641 gcgtcttatc tcaagaagag ttagcaacta tttcacaacc tgctatggca aatattttaa
    14701 aaggaatcgt gggaaattgg ggagcagcgc tgatcaatat cggcttgatt atttcgatca
    14761 ttggttcttg gttgtcttgg acgatgctcc cagcagaaac aacgatgttg atggctcgag
    14821 acggtctgtt gccgaaaggt tggggaaaaa cgaataaaaa aaatgcccca acttattcgt
    14881 tagttgttac tgcaatcttg actaatctat tcttattgac tttcttagtt actgattatg
    14941 cctatcaatt tgcctattct ttatgtacag cggcaatttt gatttgttat ctacttgtcg
    15001 ggatctatca agtccagttt tctttaaaag aaggaatcaa aaaacaagta gtgatcggtg
    15061 ggattgctgt agtttttgaa ttgatgggca tcattttagc aggattttct tatgtccttt
    15121 tatgtagtat tgcctatctt cctggttttt gcttctactg gatcgcttgt cgtgaaacca
    15181 aacatcaaat tacgggaaaa gaaaaagtaa tgattgggct gatttgtgtg gccgcactgc
    15241 tttcaatcgg gttgcttgct gtaggctgga tcaagatata agggggaaag aaacatgaaa
    15301 atcgcaaatt tcggtgtaga agaatggcta aataaatggg aaaagaaagc ggtatatgat
    15361 attgcacaaa gctcgatcga agcattaaca ttagacgaac tcgttggctt agatgggact
    15421 agcgtaagta aatttttcga aaaacgcaaa aacgaaccat tggactatgg ctggatcgaa
    15481 ggatcagatg ttttcaaaca agaagtcgct tctctttatc aaaccgtcga tccggaaaac
    15541 atcttacaaa cgaatggagc gactggtgct aatcttttag ctctatacgc gttagtggaa
    15601 gcaggagacc atgttgtttc gatgcttcct acttatcagc aattatatga catccccaaa
    15661 tctttaggtg ccgctgttga ctttgttcat ttgaaggaag aagaagattg gcagtttgat
    15721 ttagaacagc tagaagcctt agtcaaacct gaaacaaaaa tcatctgttt gaatagtgca
    15781 aacaatccta caggcacatt gctagatcgg aaaactttag aaaaaatcgc agaaattgct
    15841 cgtacagtag atgcatatgt attgatagat gaagtatatg cgcctttaac ggataaaggt
    15901 gaattcttgt cgatcgtaga tgtctatgaa aaaggtatag caaccaattc cctatccaaa
    15961 acctactcca ttccaggtat tcgtattggc tggacagcta ctggacctga gttggcagaa
    16021 gtttttcgaa agtatcgaga ctacacgatg atttgcggcg gagtcctgtc agatgatctt
    16081 gctgtacatg cattgaagaa taaagaaaaa atattggcgc gaaacaaaaa gatcattaca
    16141 gagaaccttg tgattttaaa acaatgggta gccaatgaac cgaaagttga acttgtcgct
    16201 cccaattacg tctctacttc tttcattaaa ttagccatcg aagaagatga ccagacattt
    16261 tgtatcaatt tattagaaga aacaggagta ctattagtac caggaagtgc ctttgattta
    16321 ccgaagcatg cccgactagg ttactgttgt aaaaaagaaa ccttggaaaa gggattattc
    16381 ctcttatctg agttcttgaa aaaacaggcc taaacaaaaa cactgcaagg acagtctgtg
    16441 acaactgttc aagcagtgtt tttcatttac gcttattgtc tatgagaagg agagtgccct
    16501 gaagctatgg agactcctga agctttttct tctgcagttt cttgctgatg gatctcttgt
    16561 gtattgtgtc gtacaagcaa gaagttgacc aaaacaagaa gagaagtcga aagaaagacg
    16621 ccacgataac caaatgcact ggaaacactg gagccgatca taggccctac tacgttacca
    16681 gttgcttgga aagattggtt ataactgaag atccgtccag cagcttgatg aggcgaatat
    16741 tttgtgatca atgcttggac agcagggagc agacaagcat cagagatacc aatcaaaaaa
    16801 cgtaaggcag ctaattgcca aacattcgtg acgaatgcca ttgggatata aaccagaatc
    16861 gcaaaaccca aaccaatcgc aagtattctt tcactcccaa tcttgtcacc taagcgacca
    16921 aatcttggag cggctatcaa agtggcaatc ccaggtatcg aagcaatgat tccgctgatc
    16981 aacgtaacat tcccatgtcc atgcagcaat tggcgaatgt acagactgat gattggacta
    17041 atggaattgt tggatgcttg gatgatcatc gttgtgacga acataccgat gacgacgtgt
    17101 ggatatttca agtctttgaa aatctgtttg gcagaaacca tgttttcttt tttcactggg
    17161 acaaattttt catggactaa aaacagactc aacaagaaga cgaaaaataa gatgatccct
    17221 gtaatgaaaa aggtagggcg atagccaaac gctgaagcag agatcccgcc aagaagcggt
    17281 ccaagtaatg ttcctgtaac agaaccagtt gctaaggttc caagaacttg accacttttt
    17341 tcttttggcg tcccagttgc gacaagggca gtggcattac tgatataacc agaaaaaatc
    17401 ccttgaagca gacgcaatgc gactaattga tacacactag tcacgcaacc catcaaagaa
    17461 atgacgattg ccattcctaa tgaagcacgc aacagcatca attttcttcc tttttggtcg
    17521 gctaattttc cccaccaagg agagataagc gtagtcacca agaaagtaga tgaaaaagtc
    17581 aatccgctcc aaaagttcaa ttgtgacgta ctataattac ctaacgtatc tatgtaaagt
    17641 gacataaaag gcatgactaa actaaatccg ataccagcca taaatgttcc aaaccataag
    17701 acaaacaagt tttgttccca agtttctctt ttcctaaata atttttcctt tattcttgca
    17761 gccaattaga ctccttcttt ctgaaaattt tcatctacag ctctttatta taaactacaa
    17821 tatgtagttt atcaatgaaa gtctcttttt tctctttctt tttcgtggta aactgaaaga
    17881 gtgaaggatt tattttatga gaggaaacat ggaaatgaaa aaaagaaatc tgtctcaaga
    17941 gaaaatcatc gactgctttc gagaactagc agaagagatg gatgttcagc aaataacctt
    18001 tcagcattta gccaaacgct tggatatcaa ggctccctcg ctgtataacc atttcaaaaa
    18061 tattcgtgat gtcaaaacag cgctaacagc gaaacttctg caggaactta atgaccagct
    18121 gcgacgggct cttgtgggaa aaagtggtca ggaagcactg cagatctatg cagaaactta
    18181 tcgatccttt gctttttcaa atcaagcagt gtatgagtta ctgatcagtg taccgcatac
    18241 caatgaagct gttttattag atggaatcca tgaaacgaat cagatcattt tgcagatatt
    18301 tgaggctttt caattaacga aagaagaaaa agttcatcga agcagggaac ttcgtagtat
    18361 gatccacggt tatctttcct tacgtttttt aggatacttt acaaaagaac caacaatcga
    18421 tccagaagac agctattttt ggatgatcaa taactttatc gctactttgc cttaaacaat
    18481 cagattcatt tgtaacaatt ttaagatgtt cattaaatga cccttttata agagaaaatt
    18541 cttgcaactt tcttgaaaag tgactaagat gagtctagaa aagctgaaag aaggagcacg
    18601 agtatgtatc aagtaattac catgttcggg gacaatgagc cttggtggtt ttttgaagac
    18661 tgggaagaag atattgaaga agaagaaaca ttcgagtctt tcgaagaagc acgacaagcg
    18721 tacgaaaaac aatggaacga aatccatcaa aattatgaat atatcaatgc aaaaagtaac
    18781 tttctcagcg ccttttggaa tgacggagat gagcgctggt gtgaagagtg tgacgatgat
    18841 ttacagcaat acaagggact tgcgttgttg aaaaatcatc agcccatcac agtagaaagc
    18901 agaaaggaat tttatgaaac aactaattct agcggaaaaa ccaagcgttg cgaaagacct
    18961 aagcaaggtg cttggtgcta atcaaaagca caaaaattat tatgaaggac ccaaggtgat
    19021 cgttacttgg gcccttggtc atttattagg attgaaaatg ccggaagatc tgaataagga
    19081 atggcagagc tggcaaatgg acacgttgcc aatgatccct aaaaaactag gaatcaaacc
    19141 acttcctaaa acaggacatc aattaaaagc aatcaagcag ctggctcagc gaaaagatgt
    19201 ctcagaagca gtcattgcga cagatgccgg aagagaaggg gaacttgttg ctcgctggat
    19261 cttagagtat gtgcatttca ataaaccagt gaagcggtta tggatctctt ctcaaacaga
    19321 taaagcaatc aaaactggat tcaagcaact aagacctgcg aaagaatatg atgcattgta
    19381 tgaatctgct cttgcccggg caaaagcgga ctggctggta ggattgaatg tgaccagagc
    19441 cttaactgtg aagtacgcgg ataatttatc agcaggccgt gtgcagacac cgacactagc
    19501 attagtgaga gaccaagaaa agaaaatcga gcagttccgt ccacaaacgt atttcacgat
    19561 cacgttgcaa gctggaaaag aacaagccaa aatgcttcaa aaaaatcaat ttgctttaaa
    19621 aacacaagaa gaagcacaaa aacttgttca attgatcagt acaagcaaag gaactgtcac
    19681 agaagtccaa gaaaaattaa aaacggaatc tgcgccattg ccttatgatt taacagagat
    19741 ccaaagagaa gccaatcaac gctatggcta ttctgcaaag aaaacattaa gtcttgtaca
    19801 aagtttgtat gagacccata agatcgttac ttatccgcgt acagacagca aatatctgac
    19861 aaatgacatg aaaagtacga tgaaagaacg attgcaagca gtcagcgact ttgctcctga
    19921 ggtcaagcag tatttgaaaa acggagccat cgtccaacag acaaaagtat tccaagacag
    19981 taaagtgacc gatcaccatg cacttttgcc aactgaaaac agagcacgtt atgaaaaatt
    20041 aagtaatgaa gagcgaaaaa tctatcaaat gattgtctct cgttttttag gactgtttgc
    20101 tcaaccccat aaagtcagcc aaacaaaagt tacggttgaa tttggtaaag aacgattcat
    20161 ttttcggcaa aatcgtgtcg tccaagcagg atggaaaggc gaaactgaaa ctgagacgga
    20221 aacagtcaaa tgggaaaaag gaatgagaat caatcccgat ttcgccatca aaaaagaact
    20281 atctgcaccg cctaaaccat tgtcagaagc aagcttgctt ggtcaaatgg aaaaatacag
    20341 tctgggaacc ccagctacgc gtgcagagat tatcgagaaa ctaatcaaat ctgaactgat
    20401 ggaacgtacc cctcaaggac tgcaagtatc tcctaaaggt aagcaattat tagtattggt
    20461 aaatccctct ttagtaacac cagatttgac agaaagctgg gaaaaagacc ttgaagctat
    20521 tgctgcagga aagaaacaag caggaacctt cttgaatgga atcgaaaaag aaacaaaacg
    20581 cttggtgaat gagatcaaat caagcaaaca agaatatcaa gatttctcga ttacccaaaa
    20641 gaaatgccca gaatgtgggg ccaatctgcg cgagaaaaac acacgtgacg gcaaaatcta
    20701 tgtctgcacc aattcagatt gcaactaccg cagaagaaaa gatccaaaag tctctaatca
    20761 tcgatgcccg caatgtcacc ggaaaatgga aatcatcgaa ggtaaaaacg gtgcttattt
    20821 ccgttgtaaa tacgatggaa cgacagaaaa attgcttgat aaaaaagagc agaagaagaa
    20881 aatgaccaaa catgaagaac gacgcttgct gaaaaaatat gcacaagcag aagaaccaga
    20941 agaaagccca ttagcagcag cactaaaagc agcaatgaag aagtaataag atatgaaaga
    21001 ggttgtga
//
LOCUS       DACEPD010000051        20801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.052, whole genome shotgun sequence.
ACCESSION   DACEPD010000051 DACEPD010000000
VERSION     DACEPD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.052"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..300
                     /locus_tag="IW797_001724"
     CDS             <1..300
                     /locus_tag="IW797_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1224507.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(724..894)
                     /locus_tag="IW797_001725"
     CDS             complement(724..894)
                     /locus_tag="IW797_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1224508.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1088..1267)
                     /locus_tag="IW797_001726"
     CDS             complement(1088..1267)
                     /locus_tag="IW797_001726"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224509.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1277..1501)
                     /locus_tag="IW797_001727"
     CDS             complement(1277..1501)
                     /locus_tag="IW797_001727"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224510.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            1654..2766
                     /locus_tag="IW797_001728"
     CDS             1654..2766
                     /locus_tag="IW797_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1224511.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2844..3137)
                     /locus_tag="IW797_001729"
     CDS             complement(2844..3137)
                     /locus_tag="IW797_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ1224512.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3305..3619
                     /locus_tag="IW797_001730"
     CDS             3305..3619
                     /locus_tag="IW797_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ1224513.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3640..5619
                     /locus_tag="IW797_001731"
     CDS             3640..5619
                     /locus_tag="IW797_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1224514.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5632..5958
                     /locus_tag="IW797_001732"
     CDS             5632..5958
                     /locus_tag="IW797_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ1224515.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5974..6492
                     /locus_tag="IW797_001733"
     CDS             5974..6492
                     /locus_tag="IW797_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224516.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6492..7844
                     /gene="celB"
                     /locus_tag="IW797_001734"
     CDS             6492..7844
                     /gene="celB"
                     /locus_tag="IW797_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1224517.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7928..9433)
                     /locus_tag="IW797_001735"
     CDS             complement(7928..9433)
                     /locus_tag="IW797_001735"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ1224518.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9525..10115)
                     /locus_tag="IW797_001736"
     CDS             complement(9525..10115)
                     /locus_tag="IW797_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1224519.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10294..11940
                     /locus_tag="IW797_001737"
     CDS             10294..11940
                     /locus_tag="IW797_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ1224520.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            11989..12708
                     /locus_tag="IW797_001738"
     CDS             11989..12708
                     /locus_tag="IW797_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1224521.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13187..14134
                     /locus_tag="IW797_001739"
     CDS             13187..14134
                     /locus_tag="IW797_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1224522.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14146..15066
                     /locus_tag="IW797_001740"
     CDS             14146..15066
                     /locus_tag="IW797_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1224523.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15107..16564
                     /locus_tag="IW797_001741"
     CDS             15107..16564
                     /locus_tag="IW797_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1224524.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16909..17523
                     /locus_tag="IW797_001742"
     CDS             16909..17523
                     /locus_tag="IW797_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ1224525.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17516..19240
                     /locus_tag="IW797_001743"
     CDS             17516..19240
                     /locus_tag="IW797_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1224526.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19237..20688
                     /locus_tag="IW797_001744"
     CDS             19237..20688
                     /locus_tag="IW797_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1224527.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6709 a         3510 c         4166 g         6416 t
ORIGIN      
        1 gatggcttag caccaggagc atatcagctg gtagaaactc aagcgccaac ggggtatgaa
       61 ttggatgcga caccagtaac tttcaagatc gaaaaagaac aagaagtggc gatcttttta
      121 acaaaagaga atagaaaagt tgctgatagc tcagatataa gaggaaacaa tccaacaagc
      181 agtggaaata agcatcttcc aaaaactgga gaaaccatca ttagccagtt tatattgtca
      241 atattgggtg tgctacttgt tttcatcagt ataaaattta gaaagaaacg aaatatatga
      301 gctaaaataa aaaattgaat aagcatagtt tttaaaatca actagtaccc atcaattgaa
      361 tggaaactac cgattttaga aggggtatac aaacaaatat tagtatcgga gtagagaagg
      421 ttgaacccaa ccagtaaata agtaaagaca attttgtgct gcgttgagaa gtgaaaaata
      481 aacctttgta gtaatactag ttaataaaaa atatacaaaa acactgcgaa aatcattctt
      541 gagtttcgca gtgttttttg cctaatgact caagtttatt gggtaatact ttgattttct
      601 ttctacaaat aatcagcgat cagttgtaat tttttctcta gttttaatgg ctctttgtca
      661 ataaggagaa aaacaagtcg ggtcttttgt ctcggaaagc ttcaaccaac tcgtgaataa
      721 aggttacgcc tccttgaaag gcgtggaaaa accaagtaat cgatcaatca ccaatttcgc
      781 atttggtaga acatgtttgg tgagctgaaa gtaggtggcg ttcatgtctg taactaagaa
      841 ttctatttct ttaggattag tgcagctaag gaaatagttt gttaatcgag gaattttttg
      901 tgtaggtaaa ttttctcgat taatttttct gtctcgccat ccgcggaaat aaaactcagt
      961 ttatcctcaa cggaaacgtg agaacgaaat tcacaaccct caacactcaa gacagaattc
     1021 ttttggaaga ctttggtaaa tagcttctca actctttcaa tgtgcgaata acggttgtca
     1081 aagataccta acaacttttc gaaataaaaa ataacgacac tttttcagtc agtaaagaag
     1141 tgattttatg tctgacatga tttgaaatcg agtgccttgg acggataaaa taactttgag
     1201 cgttccaatg gcttcgacag tttttacagg tatagcgctg cttttttagg cgtataacca
     1261 ttggcatatg attatattat tcaaagtgga caatggtttc cttcttcttg tttttcacca
     1321 ccattaattt cctatttcca tctaccacag cagaaccaca atttctacag acacatggag
     1381 cagggaagag aactgcagca acgatcaatg tttttttctt ctgtaaggtt tcgtaagaga
     1441 cctctgtaat catcaaattt ttgtctattg atctcagaat ttttttgata gaaccattca
     1501 taaagcatat cgttctctca gttgtttatt ttgtggtgat ttaatcatac aagaaaacga
     1561 tatgtttttt tataaaaaca tgaataggaa aaatagttga caagaaaatg acaacgccat
     1621 atgatgttcc atagtattta caacctgtca tagatgactg taaaaaataa aagtaaaaag
     1681 aaaggacgac aacaggtgga tttttatcag tctctacaac tagatccgtt tattttaaaa
     1741 caaaaaatac gagaagcagc ttctaaaaaa gaaaagtgca tgtatatttg ttcattgttt
     1801 ttacgatcat tattcatcgt attgtttgcc atttgtttta tcattataat tacaacatta
     1861 tttgaatcga agcataaacc atacgctgtg gttttattct gtatgttgat gagtattcgt
     1921 tttgttgact ttggttataa gatttcccat tcgatcatag gtttagcaat cgtcatgttc
     1981 tcactattag tagcaccgta tgtccagctg atcaaatggt cagttatggg catgttgatt
     2041 cattttattt tactaagcag catattaatg gcaaccgcta gtgatcccaa aatggggaat
     2101 gcttctttgt acggcttttc atatctcttt attgtttatt cgttacctaa agattcgttg
     2161 aacaagaatt tctttacaca aacaagtagc ttattatttt tgtttttttg ttggttttca
     2221 gttctccttt acagaaaaca tcgagaaaag aacaaagaca aaagcttgtt taaagagatt
     2281 tttttgaaag gcatgtattc gcaggaaaaa atttggatgc tcatctatgc atttggcatt
     2341 agtcttttga tcgttgcagg agagtatgtc ccttttcagc gattgatgtg ggcagggttt
     2401 gcattttctt ctattgtatc tagctatgga ttgatgtcga ttggtttcaa agaacgagca
     2461 gtagaccgaa tcattggctc attgattgga tgtgttttat ttattggtat cagccaattt
     2521 attcctttta actgggtcgg tatattaggt ggtctagctt taggtgtttg ttctacctat
     2581 agatataaaa cagtttttaa ttcttttggt gctttggcaa tcactgccag tttattcggg
     2641 gttccaggag cagtaacgat ccgcattttt gagaatatcc ttggggtatg tttaggaatc
     2701 atgtatattg gggtgactga aatacttatc cgaaaaataa gagaaaaaca cggtttgaat
     2761 cattagatga gatcgaataa aaaataagga aaaagtgaag atacacatgg atgataaatt
     2821 gctatcattc catgtgtatc tttttacggt aaatcagtga aacgttcgac tttcattttt
     2881 aatttatact ttttacggag ttcgtttatt tgagccatca tttcggattt gtgatgcttt
     2941 tcgattgctt cttcatcttt ccaacggtcg atcagtaaaa gagattctgg atcttctagt
     3001 ggaaagtaat agtcatattt ctcgtttcct tcttctgcac gaatcgcttt gacaatgttt
     3061 ctttcgacca tttcttctgc aaattttctg gcttgtccat ttgtacttgt atacaaaata
     3121 ttcactgtga tactcactaa atcccctcct gaaaaaattc tatcactagt atatcacggt
     3181 gccagaaatt attattcaag atatgataca cattttttcc gcaatgctac ggaaatatat
     3241 attttgaata gaaaacgctt acaagatatg cttggtttat aaaaataaaa ggagtgattg
     3301 atttatgaaa aaagcattga ttatttgtgc ggcaggaatg tcatcctcga tgatggcctc
     3361 aaagacaacg gatttcttta agcaaaaaag agaagaggta ctcgtagatg cagtttcagc
     3421 tacagaaggc gataatatga ttaagaatag tgattttgat ttatttctga tcagcccgca
     3481 aacgacgatg tttttggata aattcaaaaa attaggagaa tctgttggaa agcctgttgt
     3541 tagtatccct ttccaagcgt atgtcccaat ccctacaggc atccagaagt tagcagaggt
     3601 gatcgaagag aacatatgat ggaataggag tggatgataa tgaaacagaa ggaaaaattg
     3661 ctgttgagat atttgattga tcacaagaag gagtatgtca ccagtcaacg tttagcttct
     3721 gaattgttat tatctgatcg gacaatccgt aattacttgc agcggatcaa agagttagta
     3781 gaaaaaaacg gaggtaagat cattgctaaa ccaggttatg gctaccaact gcatatttta
     3841 caaagactga catttgactt gtttttgagt cggcaagaaa tagtacctgg atatgctaga
     3901 gaaccgcaag gattttatga gtccgaagat cgacaaaagt atatattgaa ccaattgctt
     3961 ttggaagatg ccattctttt tatggatgat ttggcagaag agctttatat cagtcgttcc
     4021 agtttgacaa aggacatgca agaaatcaag gagcgattga ttccatattc actaaaaatc
     4081 gtttctaagc atggtcaagg gacatggatc gaaggggaag aaagaaatcg acggcatttt
     4141 atcatggata ctttttttgg aaaccattat ggaaattcat tgaaagaata tttagggcaa
     4201 agccgttttt tcccagacat cagttttgaa gagctagtca ttatcattct cgatgaaaca
     4261 agagaagcta agttaaaagt ttcggatttt atcatacaga atctgacttt acacttggct
     4321 ttggggatca aacggctccg agaagggttt gaaatcaagg aattaggcat cgaacaagaa
     4381 gtttctaagc gagtagagta tcaagtggcg cagcggatcg ttcaacgtat tgaggtaatc
     4441 gcaggtgtat cgtttcccga agaagaaatt gcttatttga cattacatct catggctaag
     4501 tcgaatcaca aagaaaatca aaatgatcag gaactatcaa aggaattagc gaaagttctg
     4561 aaaagattgt ctcaggaatt tcatttttca ttagtagagg atctccagct tttcaatgga
     4621 ctactcgatc atttgtcacc gatgatgatt cgattgagcc gaggtattcg cattgagaac
     4681 ccattgactg aggagataaa gaaagaaaat cccaaagtct ttgacgcagt aaaaaggcat
     4741 ttcagcaaca tgccggcttt gaaaaactat accatcaatg aagatgaatg ggcctatttg
     4801 gctttgcatc tcatggcggc attggaaaaa gaaagagctg cgcataaact acatgcattg
     4861 attatctgtg caacgggtta cggcagtgcc caactattaa agaatcgcgt agtcagtgaa
     4921 ttcggtaaaa atattacagt ggtaagtgta aaaggctatt atgagatcaa tgaaacaact
     4981 ttaaaaggaa tcgacctgat tatttcttct attgatcttt cgacaatgtt tttcaaaatt
     5041 ccagttttac atgtcagtat ttttttgaat gaagatgatg ttcgtaaaat acgaaaggta
     5101 gttggagaat ctatacccta tcattcctca gatagaccga taccagcatt ttctctacag
     5161 caaaaaaagc agatttacac cgaacagttg tctgaaaaat ttttcaagac ttatacgtac
     5221 gaaccgaaaa aggaagaaat tcttcatgat ttgctgcaaa agctgagcat aaatgaagaa
     5281 gaaggatata taagagaaat gcgaaagcag atccagcaac gtgaaaatat gggacaaatc
     5341 atattcagtg atacagtggc agtcccacat cccgctgttc cagttggaat cgaaacaaag
     5401 gtagccgttg cattgattcc ttctgggatg cagtgggatc aatatcaaga aattcatttt
     5461 gtttttttag tttcgccttc ttatgtcgaa aatgaaggaa ttacggtcgt gactaaagca
     5521 atcgttaggt tagttgacca gctaaaagta cagcaggaaa tcttagctga acctattttt
     5581 gagaatttca gcagtaaatt tatcaaactg atcgaataag gaggaaaaca gatggctgaa
     5641 aaaataaaca gtgaaacact acaagtgact tcttttgata tcattttcca tagcggaaat
     5701 gcacggacac tgatacatga agcatttcgc tcgatgcgta aagagatgtt tgaagaagta
     5761 gaagagaagt tgcgacaagc aaatgaagag attcttcatg cgcatcagtc acaaactcaa
     5821 cttctgaaag aatatgccag cgggacaaaa atagaaatgg aaattatcat ggtccatgca
     5881 caagatcatc tgatgacgac gatgacatta ttggaagttg cacaggaaat gaattatttg
     5941 tatcaaaaaa ataaatagaa aggaaggatc attgtgcagg atacagtaaa aaggaaaatc
     6001 gaattagaaa aaaagcaact tagtttgaaa aacatgtatt tcaatcgtta tctaatcgta
     6061 cgttacctga ctgccttttt ctttttcatg aacatgcaat ggatgattct gcttctatca
     6121 gctaaaagtt taggaagtag tttaccgatg attctgattt tagctatttt gcctgcagta
     6181 ggagaacaag taaagcttta tcgaaaacac caaacgaacg ttccatggac aaagtggtat
     6241 tttttgttcc agagtgtctt taatttatta gtgattccag tgttgtttac ctcaggattt
     6301 acattcttgt atccttttat ggcaaataac aatggaggac agttatttgt tttcattctt
     6361 gttgtaagcg gtatttttgc cagtgcattt atacagtatc gtttaaagaa aatttcactc
     6421 aaccaagacc aacagtatat ccgtattaaa caatatgaaa aagcgttata tttaggaaag
     6481 gagaataact aatgtttgat tttttgcaga aatacctgat gggacctatg gggaaaattg
     6541 cgcaacttaa aatcgttcgg gctgtcatgg cagcggggat ggcttctatt cctttcacga
     6601 ttgtaggctc gatgtttttg gttttaaata ttttgccatt accgttccca tttttagaag
     6661 gattttttaa tgcaactttt ttcaaagtta gtgacttgta catgatcgtc aatacaatga
     6721 cgatggggat tttatctatt tactttgcca ttgtgtttgc ttatgaatta acgtcaattg
     6781 aacgagatga acaaaaatta aatgttaatc cgctcacggg ggcattacta tctgtttttg
     6841 cattctttat gtgtatccct gaattgatca tgactgatgg taaaatcagc ttgattgctt
     6901 ctatcaatga gacagaaact gtagtaaatg gtattaggat gggctctttt gtcgaacgtt
     6961 taggaacttc aggaatcttt acagctatta ttatgtcgat tattgcggtc gaattgtatt
     7021 gtatgtgtgt caagagaaat tgggtcataa aaatgccaga tgttgttccg ccaggagttt
     7081 ctcgttcttt cactgctctg atcccaacat ttgtcattgc gtttgtggtg atgatcatca
     7141 atggattatt agttgcacta ggaacggata tcttcaaaat gattgctatc ccatttgggt
     7201 ttgttacgca gttgacgaat acatggcttg gtgtcatggt gatttatttc ttgatccatg
     7261 cattgtggat cgtagggatc catggagcga atatcatcac ttctttcttg accccgatcg
     7321 tattagctaa tatggcagct aacgcacaag gcgcaaatta tccgttggca ggagaattca
     7381 acaattctta tgtaaccgtt ggtggatcag gtgcgacttt aggattgatc atctttattg
     7441 cattcctcgc gaaatctgat cagctgaaag tgttaggaaa agcctcactt gtaccaggat
     7501 tattcaatat caatgaacca atcattttcg ggatgccaat cgtctacaat ccctatttag
     7561 cggttccgtt tttcttagct ccgatggctt ctgctacact cgcttatttt gccattcggc
     7621 tggaaatcgt caaaccgatg cttgcacaga tgccgtggcc ttcaccagtc gggatcggag
     7681 catttgttgg tagtggtggt gattggaaag ctgctgtgtt agcagtcatt tgtgcaatca
     7741 tcgcattcat tatctggttc ccatttatca aattctatga taataaatta ttaaatgaag
     7801 aaagagaaaa ggcagcagaa ttagctagtg aaaagttaaa atgatttcta agaaaaaagg
     7861 aactgtgaca aaaaatcaca gtcccttttt ttcttagaaa tgggaaacca ctagtatata
     7921 aataccatta ttcttcaatc aaccgcttca cgattgcata atccccttca taaggttcat
     7981 ctacaccatt gattttttga tagggatcca cgccttttcc cgcaataacg actgaatctt
     8041 gcggagcgct ttctgctaaa gcgcgtcgaa tcgcttcagg acgatccatt tcataatgaa
     8101 cgatcaaatc tggattagta attgcttcat tgatttcttc cgcgattttt ttcggatctt
     8161 caaatgctgg gtcatctgcc gtcaaaaagg cgatgtcagc tgtttctgat agcacttttc
     8221 caaaatcatg tcgacgggag attgctttgt ttcctgggct gcctaataca acaattacac
     8281 ggccatctgg atgttcattt ttggcaaatt tcagcaatgt tttcaagctt agataattat
     8341 gtgcatagtc cacatagaca tgcgctccat tggattggat caattgatcc attcggcctg
     8401 gtacacgagc ttcttttaat ccgtcttgtg catcttgact ggaggcacct gttagtgctg
     8461 cggcaatgat cgcactggct gcattttctt ggttgaatcc tccagccagc aaaatatcat
     8521 aagcagtatc gcagatagct aagcgatctt catgagaaga taaagtaaaa ccgtgagtac
     8581 ctttatctga acggatcact tgataattgc tttcttccga acgaccataa gtgatcgttg
     8641 gaatctggaa tagatcggct gtttccctaa gtaaatcaaa atagtcactt tcatgattta
     8701 aaacaactgt ttttgaattc aagatcaatt gtcgcttgca atagaaatag tcatcaaatg
     8761 tcggatgttc gatcggactg atatggtcag gcgagatatt caagaagatt ccgacatcta
     8821 aagtcaagcc gtatacgcgt tgtgttttgt aagcttgtga agaaacttcc atcactagat
     8881 gagtcatacc attttcaact gcttctgaca tcatacgata taaatctaat gactcaggcg
     8941 ttgtcaaatg ggatttgaaa tatgttttgc catctaacgt tgtattcata gtagaaagca
     9001 atgctgtttt tttatttgtt gtgtgatcca agattgcttt tgtaaagtac gcagcagttg
     9061 ttttaccttt tgttccagtg aatccgatca ctttcaattt attttgcgga tagtcgtaaa
     9121 aagccatagc aagaagcgcc attgctttac ggatatccgt tacgatgatt cctactgctg
     9181 aattttcgta tggttgttct gatacataat attgaatacc ctgttcgatt gctgaatcca
     9241 aatattcttc tttgaaattc attcctttac agaaaaacaa agtagaagca tcagcttttc
     9301 ttgaatcata acttaactga agaaattcag ctttttctgg tacatcaaga tgccagcctc
     9361 ttgttgaaac aaattctttt aatagatttt cttttaatag taaatgacgg atgtcttcta
     9421 aagagaagct cattgattga tccctcgctt tattcaattt cttggtttat tatagcacac
     9481 tctttttaat atatcgtttg tttccgctat gacaaaatcc agctttattc aaattttctt
     9541 gacacagggg acaagcggat gagtattttc ataaacaatg aactgactgt aatagatagg
     9601 agaacgaata gtgtctccaa caatccaaaa acataataat tcaaaaagac gataagacta
     9661 ttggtaagta aaagtccttg cggcattgga agccgaaaaa atttgtggaa aataatcggc
     9721 ggtatcgtta taccgccatt tgaggccccg actgtgtaca atgtgtaaac cccaagactg
     9781 aacaaaagac ttcctgaaat cagtgaaaag aaatgtgagg agatcatttc cacttttgga
     9841 acaatctcta aaacaacagg aaaaagcaga cttcccgcta cagtcttaaa aaatactgat
     9901 tttcctaata ggacagcggc tagaagcagc atcataagat tgatgctcca aacagtaact
     9961 gagcgttgga tagaaaaggc cgtttcaact aaatgtccga tggcgcccac tccggcggaa
    10021 gctatatcgt gagggagcaa gaaccaattc aatccaactg tgataaagaa aagacctaaa
    10081 aatataacgg gtagtttttt tatttcttgt gtcattgata tctcctataa atgttagcca
    10141 taaaaggctg tatatttctt tactttacca aaaaaacgaa accgaattca aaaaaacaat
    10201 tgttcttata aaagttatat aaaatcgatt gccagtttct gttttaccaa ttaaaatgat
    10261 agtaatgttt tgtagaaagt tggaatctga ccgatggata atcaaagaaa accagcatta
    10321 gatcaattaa caccgcaaga aaaaatggca cgaggctctg cttggatgac cgccagtaat
    10381 atcatttcaa gattgctagg cgctgtctat atcattccct ggtatgcttg gatgggcgaa
    10441 aatgctaagg ctgccaatgg attattcaat atgggataca atatatatgc attgtttcta
    10501 ctgatctcta cagcaggaat acctgctgcg attgcaaaac aaactgcacg ctataattca
    10561 ttgaatgaat atggcacttc acgacgatta tttatccgtg cattacagat gatgggcggg
    10621 ttaggcctgt tgtttgcttt gttcatgtac atcgcctctc cttggctagc tcatgcttct
    10681 ggtggcggag aagaactgat tccaacgatg cgttctttga gtatcgctgt attggttttt
    10741 ccttgtatga gtgtgatacg agggtatttg caaggcaatc aagaaatgat gccttatgcg
    10801 ctgtcgcaga ttgtagaaca ggtggcacgt attttttata tgctattggc tacattcatc
    10861 atcatgaaag tcatggatgg tgactatgtg acggcggtca cgcaatcaac tttcgcggca
    10921 tttatcggta tgctagcaag tattcttgtc cttttgtatt ttttgaagaa acatcaagcg
    10981 tatacatcgg cgtttattca ctacagcgaa aacaaaataa atattgcaac gaaggaattg
    11041 ttgttagata ccgttaaaga agcgattccg tttattattg tgggttcagg ggtaacgatc
    11101 ttcaaattag tcgatcagtt cacttttatg aagatcatgt ctgcatccac cgagtattcc
    11161 aatgcacagt tactggatct tttttcgatt tttagtgcca atccagataa gttgacgatg
    11221 gtagttattg cattggcaac atcgatcgcg tctactgggt taccgttgat tacagaagct
    11281 gttacgttga aagatcgagt aggtctggca aaattgacaa gcggaaattt acagttgttt
    11341 tcatttttta tgtttccggc gacatttggt gttatgttac ttgcctatcc gctaaataca
    11401 ttattttata caccggattc tctaggaagt aatgtactga tccaagcaag ttttgtagga
    11461 ctcttcctag gactatatat gctagtttcc aacatgctgc aaggaatgtt tgaaaacaag
    11521 gcggcaatta attatttggt tattgggttt ctagtgaaac tccttcttca atacccagcc
    11581 attcgtttat ttgaagtata cggcccttta ttggcgacaa tgatcggatt tgctgtgtct
    11641 tgtaccttga tcttaaagag aatccatgaa gtagcccgct ttaaaccaag ttttgtctgg
    11701 cgcaggacat tgctgatctt tattcttacc ttgattatgc ttttggcttc gtggatcaca
    11761 aagatgattt ttggtgcttt tttaaacgaa gatagcaaat tccagtctct attgttgatt
    11821 cttgtagtag caggtatagg ggctttagtg tatggttatt tagctttgaa aatccgcttg
    11881 gcagaaagac tactaggacc aggaatagcg cgtttacgac ataaattaaa aataaaataa
    11941 gtgaatcaga agagctagat tgatgctctt ttgaaatggg ggaaacgcat gcgtttagat
    12001 aagtttttag cagatatggg acttggcagc cgcaaagaag tcaaaggatt aatcaaaaaa
    12061 ggaacgatcg aagtcaatgg acaagtggtg aaaagcgata aagcgcaagt aaatgaattc
    12121 gaagatgcgg ttacttattt aggagaaatc attacgtatc aaaaagattt ttactacatt
    12181 ttgcataaac ctgcaggagt catttctgct actcaggata atcatgatca aaccgtaatc
    12241 gatttattgg aagaccagga ttatcgggaa gatctttttc cagtcggccg attagataaa
    12301 gacacagagg gcctcttgat tttaacgaat gacggggtgt ttgcccatca gttactatca
    12361 cctaaaaaac atgtggagaa agaatatcta gctgaggtta gcggtataat gacagaagaa
    12421 gatatccgtt tatttgcaga aggactgatt attgatggcg atgagcagac attgccggca
    12481 aaactccaaa ttgatcaagt agatcaagag acagaaactt caattatccg tctgattttg
    12541 catgaaggaa agtttcacca agttaagcga atggtcaaag cagtaggaaa agaagtctta
    12601 tatttaaaaa gaatccgtat gggaaaattt tggctgccta aagatttgaa taaagggcaa
    12661 tatcgaccca tgaccaaaga agagctagaa caagtgagag aaaaataatt gaatggatag
    12721 ctaaaagaat aagcagagta gttgtagcgc catctgaaaa gcagacttgt ccgtctgact
    12781 ttcggatggc ttttttgtga aatttcggct tatttccgta gaagctactt ctggaacacc
    12841 gtttgttcgg aataagggac tgtgacaaga cttttgttac agtccccatt gttccttgtg
    12901 actcagagtt agatagtaag gacacgatct ccctaatact caggccaaaa cgctgaattc
    12961 acaacctctg ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    13021 aaatatgagg aactattttc ccgttttttt ctctaatact caggccaaaa cgctgatttc
    13081 acaacctctc ctatactttg tttcattttc cctatacttt gtttatttat tctcattatt
    13141 gaaagcgcta taattacgtt gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa
    13201 ggggttcaat cattttttta tgaatctatg gcgttataaa gcacttgtcc tgatggctgt
    13261 accgggtatg ctttggatga tttttttctt ttacatacct gtaatggcga atgtcgtagc
    13321 attcaaaaat tttcatattt cagcagatgg gttcttggct agcttgaggg aaagtccttg
    13381 ggtgggttgg gaaaatttca aatttttatt ttcttcggat caagcatttc tcatcactaa
    13441 aaatacaatt ttatataatg tagcatttat ccttttgaat ctgctgatct cagtcgtatt
    13501 tgcaatcatt atgagcgagt tgagaaataa acgactcgtt aaagtttatc aaacgatgtc
    13561 cttactacct tattttctat cttgggtcat tatcagttac tttgtttacg cttttttaag
    13621 tcctgacaaa ggaatattca atcagtggat cactggacat ggaggagaag ctgtcaattg
    13681 gtataatgaa ccgaaatatt ggccgtttat cttggtgttc attggtacat ggaaagggat
    13741 tggttataac agcattattt actttgcatc tgttatggga atcgatccga cttattatga
    13801 agctgctatg gttgatggag ctagcaaatg gcagcagatc aaacatgtga cgataccaca
    13861 acttattcca ctaatgacga ttttaacgat tttagccgta ggaaacattt tccgagctga
    13921 ttttggcctg ttttataaca ttcctaggaa ttccggtgca ttatatgatg tgactcaagt
    13981 tttggatacg tatatctaca atggcctgac atccacagga gattttggta tgacggctgc
    14041 tgcaggactt taccaatcag ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc
    14101 ccgacgtttt gatagcgatt ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa
    14161 gtggagagag tccaagtccg ctctttcaat aagacggcta attttttctc taatataatg
    14221 attgcactct ttgctatttc ctgtattttg ccatttatat ttgttatcgc catctctttt
    14281 accgatgagt ccatattgtc gactcacggg tacagttttt ggccagaaaa gttcagtata
    14341 tttggttata cgtacttgtt cgaccagatg caggataaaa ttttccaagc gcttttcgtc
    14401 actgtacttg ttactgtttt aggtacactg atcaatagca cggctacttc tttatatgct
    14461 tatgcgattt cacggtcaaa ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt
    14521 acgatgttgt tctcaccagg gatggtggcc aattatcttg ttatgacaaa tcttcttcag
    14581 ttaaaagata cgatttgggc attgatcttg ccaatggctg tcagtccctt caatattatt
    14641 gtgatgcgca cattctttaa acgttcggtc tctgattcga ttattgaatc ggcacggatc
    14701 gatggagcta gcgaactgcg aatatttgtt cagatcgttt taccgttagc tgttccaggt
    14761 attgcgacaa tcagtttatt tgccgcattg ggttactgga atgactggtt caatgcttta
    14821 ctatacatcc aagatgataa gctagttcca ctacagtatc ttttgatgaa aatccaaagt
    14881 aatattcaat atctgacaca aaattcagga gcaggaagcc aattgacagg cggattagcc
    14941 tcagttccag gagaatctgc cagaatggcg atcgtagtca tctcgacgtt gcctattgcg
    15001 atcagctatc catttttcca gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa
    15061 gaataaagaa acaaaaatat aaatcataaa attaggagga agaaaaatga agttatggaa
    15121 gaaactagcc tttacaggtg tttcagcgtt gttgttggga acattagcag catgcggagg
    15181 ttctggttct aaagaccagg cagaagctag caattcggac actttgcaga tgtatcaaat
    15241 cggagataaa ccagataatt tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa
    15301 agagatcggt gtaaaagtca atatcaatta cattggctgg ggcgattacg aaaagaaaat
    15361 gaacgtcatc atttcttccg gggaaaatta tgatatcgcc tttgcgaaca actatgtttc
    15421 caatgcccaa aaaggagcct ttgctgattt gactgaatta gcgcctaagt acgctgaaaa
    15481 agcttataat gatttagatg aagcatatat taaaggaaat ctggtcaatg gaaaattata
    15541 tgctttccca gtcaatggaa atgtctttgc tcaacaagta cttactttta acaaagcttt
    15601 actagataaa tacgatcttt caattgatgg aatccaatct tacgctgatg cagaaaaagt
    15661 cttgaaggaa ttccatgaaa aagaaccaaa cacggcagct tttgccattg gacaaggctt
    15721 caaagtccaa gggaactttg attatccttt agggaatacg ttaccatttg ctattgatct
    15781 ggatggagat gattcgaaaa tcatcaacca atacgataat gaaacgtata tcggactgtt
    15841 acgtacaatg catagctggt acaaacaagg actcattcca agtgacgctg caacgagcaa
    15901 taaggattat ccactagaag gaaatacttg gctaatgcgt ggagaaacac aaggaccgta
    15961 tgattatggc gatacaattt tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat
    16021 tactgttcca ctaaaatcta cagctcaagc acaaatggca aattttgtcg tatcaaatac
    16081 ttcaaaaaat aaagaaaaat cagtagaact tctagggctg cttaacagtg atccagaatt
    16141 attgaatggg cttgtttggg ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga
    16201 tgataaaatc aaattgctag atggttatca gccaaataca catatgtctg cttggaatac
    16261 aggaaacaac aagattctat atacacaaga gtctattacg gatgacatga ttgccaaacg
    16321 tgatcaatcg attgctgatg cagaaacttc accgatttta ggattcagct tcaacactga
    16381 cagtgtcaaa acagaattaa gtaatatttc taatgtcatg aatcaatatt tagatggatt
    16441 gaatacaggg actgttgatc cagatgaaac attaccgaaa ttgaaagacg ctttggacaa
    16501 ggctggctat gataaggtat tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga
    16561 ataataatga ataaagacaa aggggttgtg acaaaagacg tgtcacaacc tcttgttccg
    16621 aataaacggt gttcaaaagc cagtttctcg gtattaagct aaatatatgt aaaaatgtgg
    16681 aaagccatat tcacaaaatt tttcttacta ctcgaaacta agcggctatt tcacaaactc
    16741 ttggtaaacg gtgttcaatc agctaaccct cggtattcgc tcacaaaacg acaaaatatg
    16801 aggaactatt tttccgtttg tttctctaat aatcaggtca aggtgctgat tgcccaacct
    16861 ttttttgtat aatgagaaag tataaactta atgtttggag gagaagatat gttagggaag
    16921 gttttggaag agttatttat ccggatctgg gtcattataa aactaacctt gtatttctgg
    16981 atatatacat ttgccggcgg gatcatcttt ggattaggtg cggcatggaa aacagtcaat
    17041 gaactatttt atttgtacgg attcgagtat aaagaaatca caataaaaag aggctggaat
    17101 atatataaaa gaaatttttt aagaggcaat ctgctattta gtttattttt atctggtact
    17161 gctttattga gttacaattt atatttatct gttcaaatca aagggctcat ttttttagct
    17221 attgatttta ttttactttt tgccttgctc tgcctatttg ctacttatca atacagtatg
    17281 attttagata gtgaatatac gatctctgtt cctaatctgc taaagctaag ttttatttcg
    17341 gttttttcca gcttttcgtc ttttctaaaa ataattattg gtagcggtat aattttaggc
    17401 gtgacttggc aattcaaagg attgatcctg tttggtgtca ttggtctttt gacggtttgg
    17461 aatggtacga tgacaacaca ttggcgtgaa gaattagata aacagttgga aagttatgaa
    17521 taagtttcgg attctatttt caaaaggaac cttactaaat cgtctgttgc ggaaatacgc
    17581 ggttatcatg atcggtgtaa cgatgactgc taccgttatt tttagtatcc atacttggga
    17641 acaaaatcaa aaacaggcag aaaacatgac aagtgatgcc gttcaaagca caagcagaat
    17701 gctgaatgat aaaacaacct tgagtaggat catcaaaaac cagcttgtag ggaattccga
    17761 aaagatcgaa aatgtgacga cctacttaac aaaacccata gatcagtatc tgatgtatgt
    17821 atacgaacag caaaacagta ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct
    17881 ctacgcaaat tatgaagaac taagtgcgat atatatcgtg ttgaatcaac taccggagta
    17941 ttatgaatct tctagagaaa acaaaggtgg ggaaataaaa aaggggacac caaaattact
    18001 agacgccttt tatatcaagt tgcctatcac acaaggagga gctgagtcag gaagtatttt
    18061 tattggtttt caaaagaaag aactagatat gattttagaa aatctaacgt cattcaatgg
    18121 actctcttta tacatgattt ctggaacgac aaatcgacta tacacattcc atgataataa
    18181 aatagctaaa gaaacgtttg aaagacaaga aatgatcatc acagagagtc tgaagaaaaa
    18241 ttcacaattg ccgattgacg atctggaaaa aaacaatttt gttcaacacc aagaactgtc
    18301 tgatgattat cgtatattag ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa
    18361 aaatcttgcc ccgctgatca ttggtatcat tattttagac aatatactgc ttatctttct
    18421 ttataaaaca ttcaaacgat attcacagca agtatctatc gtcatgcagg ccatggatca
    18481 aacagcagca gggaatttag agacgcggat cgacataaca aacacggaat atgaactgaa
    18541 ggaactttca atcggtatca acgaaatgtt ggatagcatc aatcagtttg tagaagacat
    18601 ctacaaatta gaaatcaaac agcaagacgc acatatgcgg gcgcttcaag cacagatcaa
    18661 tccacacttt ttatataata cattagaata tatccgaatg tatgcaatca gcgaaggaag
    18721 tgaagaatta gcagatgttg tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca
    18781 agagaaaacg atcactttaa aagaagaact ggacttttgc gaaaaatatg tctatttgta
    18841 tcaaatgcgt tatcctaatc gagtagccta tcattttatg atcgatcctg atttggaaaa
    18901 gatcgaagta cctaaatttg tgatccagcc attagttgaa aattatttta aacacggtat
    18961 tgattttact cgattcgata atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt
    19021 aaggatcatc atcaaagata atggaaaagg gatgactgaa aaacggctaa aacagattga
    19081 agaaaaactg tctcatccta aagtggaact tcacggttct atcggtctgc aaaacgtgaa
    19141 tgaacgttta cgtgccagct tcggcagcag ttattatatg tcgcttgaga ataatgagac
    19201 aggtggactg accgtttcta ttacattcaa ggagggatga gcgtgtatca agtgttattg
    19261 gtagatgatg aatacatgat tttgaatggg ttaaaaaaga taattgattg gcaaagttta
    19321 ggctttcaaa tagtagcaac agcagagaat gccaaagaag gactagctgt acttgaacag
    19381 cgacagattg atttagtagt aacggatgtg acaatgccag aaataaatgg actggaattt
    19441 attgaagcgg ctcaaaaaga acgtcataat tttgaattta tgattttatc aggataccaa
    19501 gaattcgatt atttgaaagg aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc
    19561 gtgaataagt tcgaattggt tgaaagcctc aagaaaatca aaacacggtt agatcagcaa
    19621 aatgaacaaa aaaatcaaca agaaatctat caagaactgt tattcagtca atggttgaat
    19681 gaagaattgg atgaaataag tgaagaagaa ataataggac aaataagtga gaagcagcat
    19741 aggatagtat tgattcaact ggcaagaact catgatactc ttatcaatag atggttgcaa
    19801 gaacggcaac aaatctttta ctatcaacga aattacggag accgtgtatt gatcacgttg
    19861 ttgttggagg cagatgaagt tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat
    19921 caggaatggc tgatcagtat cggggaagag acagattctt tggacaaaat ccctgaaaca
    19981 ttccaacaag cgaaagacaa tctacagttg catcaatttt atggagataa agacgagcat
    20041 gttttttacg cagtacaagc agaaacaaaa gatcaaacca tcgatttttc aagtttcaga
    20101 cgcgtgctgc aaaataaaca attggaagtc gctcaaaaaa tgatcactga tttttttgaa
    20161 cagtttcagt tagcagccat gatgccggaa gatatacgat attccagttt tttactgttt
    20221 atggagattc aacgggaatt gattgatctg gaagatgagg aatatttaca aggaatcgaa
    20281 aaaatccatc aggcaaaaac ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa
    20341 aagcatcaga gacagaaaaa gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac
    20401 cagcattatc aagaaccact tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc
    20461 atgtatctag gacagctgtt caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac
    20521 catttacgta tggaaaaagc gaaacaactg cttttgcaca gtaatcaaaa catcaatgag
    20581 atagcaagtg agattggcta caacaacacc acgtatttct ctaaattatt caagaaaatc
    20641 gtcggcagat cccctaaaga gtaccgagaa aacagtggag aatcataaaa gaaaaagcca
    20701 atgtaatgaa ataacttata aaaaattgtc tagaaatcta attttgggct gttcttatta
    20761 cttgaggcaa ggcagctttt tcatgacctc tcgataaacg g
//
LOCUS       DACEPD010000052        20268 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.053, whole genome shotgun sequence.
ACCESSION   DACEPD010000052 DACEPD010000000
VERSION     DACEPD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.053"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            292..1431
                     /locus_tag="IW797_001745"
     CDS             292..1431
                     /locus_tag="IW797_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ1224528.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="IW797_001746"
     CDS             complement(1498..2073)
                     /locus_tag="IW797_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1224529.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="IW797_001747"
     CDS             complement(2078..3052)
                     /locus_tag="IW797_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ1224530.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="IW797_001748"
     CDS             3335..4303
                     /locus_tag="IW797_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1224531.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="IW797_001749"
     CDS             4416..4694
                     /locus_tag="IW797_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1224532.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="IW797_001750"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="IW797_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ1224533.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5160..5906
                     /locus_tag="IW797_001751"
     CDS             5160..5906
                     /locus_tag="IW797_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1224534.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="IW797_001752"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="IW797_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ1224535.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="IW797_001753"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="IW797_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ1224536.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="IW797_001754"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="IW797_001754"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ1224537.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="IW797_001755"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="IW797_001755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ1224538.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="IW797_001756"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="IW797_001756"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ1224539.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="IW797_001757"
     CDS             10643..11185
                     /locus_tag="IW797_001757"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ1224540.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="IW797_001758"
     CDS             11208..12764
                     /locus_tag="IW797_001758"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ1224541.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="IW797_001759"
     CDS             12778..13680
                     /locus_tag="IW797_001759"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ1224542.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="IW797_001760"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="IW797_001760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ1224543.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="IW797_001761"
     CDS             15123..15545
                     /locus_tag="IW797_001761"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ1224544.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="IW797_001762"
     CDS             16014..16244
                     /locus_tag="IW797_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ1224545.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="IW797_001763"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="IW797_001763"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1224546.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="IW797_001764"
     CDS             17655..17831
                     /locus_tag="IW797_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1224547.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="IW797_001765"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="IW797_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ1224548.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="IW797_001766"
     CDS             18326..18709
                     /locus_tag="IW797_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1224549.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="IW797_001767"
     CDS             18878..19762
                     /locus_tag="IW797_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1224550.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19879..20112
                     /locus_tag="IW797_001768"
     CDS             19879..20112
                     /locus_tag="IW797_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ1224551.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
BASE COUNT         6739 a         3539 c         4404 g         5586 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatc
//
LOCUS       DACEPD010000053        19496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.054, whole genome shotgun sequence.
ACCESSION   DACEPD010000053 DACEPD010000000
VERSION     DACEPD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.054"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            168..362
                     /locus_tag="IW797_001769"
     CDS             168..362
                     /locus_tag="IW797_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224552.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IW797_001770"
     CDS             631..930
                     /locus_tag="IW797_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224553.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IW797_001771"
     CDS             complement(1027..1461)
                     /locus_tag="IW797_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1224554.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IW797_001772"
     CDS             complement(1693..2403)
                     /locus_tag="IW797_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ1224555.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IW797_001773"
     CDS             complement(2393..3253)
                     /locus_tag="IW797_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ1224556.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IW797_001774"
     CDS             complement(3258..3896)
                     /locus_tag="IW797_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ1224557.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IW797_001775"
     CDS             complement(3911..4210)
                     /locus_tag="IW797_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1224558.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IW797_001776"
     CDS             complement(4253..5737)
                     /locus_tag="IW797_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ1224559.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IW797_001777"
     CDS             complement(5757..6218)
                     /locus_tag="IW797_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1224560.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IW797_001778"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IW797_001778"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ1224561.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IW797_001779"
     CDS             7521..8291
                     /locus_tag="IW797_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1224562.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IW797_001780"
     CDS             complement(8564..9391)
                     /locus_tag="IW797_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1224563.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IW797_001781"
     CDS             9959..11194
                     /locus_tag="IW797_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ1224564.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IW797_001782"
     CDS             complement(12193..12453)
                     /locus_tag="IW797_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ1224565.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IW797_001783"
     CDS             complement(12480..13781)
                     /locus_tag="IW797_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1224566.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="IW797_001784"
     CDS             complement(14073..14531)
                     /locus_tag="IW797_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1224567.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15869)
                     /locus_tag="IW797_001785"
     CDS             complement(14544..15869)
                     /locus_tag="IW797_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ1224568.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17298)
                     /locus_tag="IW797_001786"
     CDS             complement(15886..17298)
                     /locus_tag="IW797_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1224569.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="IW797_001787"
     CDS             complement(17315..18079)
                     /locus_tag="IW797_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ1224570.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="IW797_001788"
     CDS             complement(18183..19058)
                     /locus_tag="IW797_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1224571.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="IW797_001789"
     tRNA            complement(19313..19397)
                     /locus_tag="IW797_001789"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
     gene            complement(19415..19486)
                     /locus_tag="IW797_001790"
     tRNA            complement(19415..19486)
                     /locus_tag="IW797_001790"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19452..19454),aa:Gln,seq:ttg)
BASE COUNT         6204 a         4011 c         3006 g         6275 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
    19441 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaaaa
//
LOCUS       DACEPD010000054        19452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.055, whole genome shotgun sequence.
ACCESSION   DACEPD010000054 DACEPD010000000
VERSION     DACEPD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.055"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            133..3660
                     /locus_tag="IW797_001791"
     CDS             133..3660
                     /locus_tag="IW797_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ1224572.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IW797_001792"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IW797_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ1224573.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IW797_001793"
     CDS             7448..7789
                     /locus_tag="IW797_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1224574.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IW797_001794"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IW797_001794"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ1224575.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IW797_001795"
     CDS             9146..11566
                     /locus_tag="IW797_001795"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ1224576.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKEINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IW797_001796"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IW797_001796"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ1224577.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IW797_001797"
     CDS             13000..13737
                     /locus_tag="IW797_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224578.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IW797_001798"
     CDS             13751..14566
                     /locus_tag="IW797_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ1224579.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IW797_001799"
     CDS             14579..15226
                     /locus_tag="IW797_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1224580.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IW797_001800"
     CDS             15242..15898
                     /locus_tag="IW797_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1224581.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IW797_001801"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IW797_001801"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ1224582.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IW797_001802"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IW797_001802"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ1224583.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IW797_001803"
     CDS             18240..18758
                     /locus_tag="IW797_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1224584.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IW797_001804"
     CDS             18865..19365
                     /locus_tag="IW797_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1224585.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6539 a         3340 c         4176 g         5397 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaaga
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga ca
//
LOCUS       DACEPD010000055        19308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.056, whole genome shotgun sequence.
ACCESSION   DACEPD010000055 DACEPD010000000
VERSION     DACEPD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.056"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            968..2218
                     /locus_tag="IW797_001805"
     CDS             968..2218
                     /locus_tag="IW797_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ1224586.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            2237..3247
                     /locus_tag="IW797_001806"
     CDS             2237..3247
                     /locus_tag="IW797_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAQ1224587.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDVHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFEDGGTRVIHYTPETYIEKQ
                     NG"
     gene            3257..3910
                     /locus_tag="IW797_001807"
     CDS             3257..3910
                     /locus_tag="IW797_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1224588.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            3929..4927
                     /locus_tag="IW797_001808"
     CDS             3929..4927
                     /locus_tag="IW797_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1224589.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWKPTVSLEEGLRESYNWIEKQIKG"
     gene            5003..5764
                     /locus_tag="IW797_001809"
     CDS             5003..5764
                     /locus_tag="IW797_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAQ1224590.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            5782..6888
                     /locus_tag="IW797_001810"
     CDS             5782..6888
                     /locus_tag="IW797_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1224591.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRDVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            6878..7111
                     /locus_tag="IW797_001811"
     CDS             6878..7111
                     /locus_tag="IW797_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224592.1"
                     /translation="MINDMEVTKLMALRNRYALNIVDNCTRKIAKILGCCIGKGAQIG
                     NSVEFVHNSVGTVIHSDTILEDGVKVYQNVTCG"
     gene            7310..7765
                     /locus_tag="IW797_001812"
     CDS             7310..7765
                     /locus_tag="IW797_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAQ1224593.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            7779..8552
                     /locus_tag="IW797_001813"
     CDS             7779..8552
                     /locus_tag="IW797_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1224594.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            8539..9669
                     /locus_tag="IW797_001814"
     CDS             8539..9669
                     /locus_tag="IW797_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1224595.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            9700..10935
                     /locus_tag="IW797_001815"
     CDS             9700..10935
                     /locus_tag="IW797_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224596.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFVPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLSVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            10960..12069
                     /locus_tag="IW797_001816"
     CDS             10960..12069
                     /locus_tag="IW797_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224597.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAKYEKYMDFVWEE"
     gene            12111..13445
                     /gene="gmhB"
                     /locus_tag="IW797_001817"
     CDS             12111..13445
                     /gene="gmhB"
                     /locus_tag="IW797_001817"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAQ1224598.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            13480..15117
                     /locus_tag="IW797_001818"
     CDS             13480..15117
                     /locus_tag="IW797_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ1224599.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            15219..15614
                     /locus_tag="IW797_001819"
     CDS             15219..15614
                     /locus_tag="IW797_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ1224600.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            15632..16363
                     /locus_tag="IW797_001820"
     CDS             15632..16363
                     /locus_tag="IW797_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224601.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            16387..17838
                     /locus_tag="IW797_001821"
     CDS             16387..17838
                     /locus_tag="IW797_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ1224602.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            17864..18475
                     /locus_tag="IW797_001822"
     CDS             17864..18475
                     /locus_tag="IW797_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ1224603.1"
                     /translation="MIDIISAIMVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            18657..19172
                     /locus_tag="IW797_001823"
     CDS             18657..19172
                     /locus_tag="IW797_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-keto-6-deoxy-D-glucose epimerase"
                     /protein_id="HAQ1224604.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNGERLSF"
BASE COUNT         6223 a         2699 c         4217 g         6169 t
ORIGIN      
        1 ttaccaacat gtttagacat tttcctctag gaaaaaatta actagcacca cgggtgatat
       61 atatgtatat ggtgactgaa gacaaaaaaa cagaactaat gaaactgaat aaaaatatag
      121 agtgaactag tttctaagag ggattttaaa ctagatttca ttggtaataa aatttggaga
      181 gatggaaaaa cagcacttaa ggaagcagat tttgttgtca tttttgttcc tacaaactat
      241 ggtagtgaat gtgttattga attagtgatg tctgttattt cagaggcatt ggggataatt
      301 aaatccacta tacctgtagg atatacaaaa agtggaagaa aaaaatatta tcaatagatt
      361 ttatttagtt cagaattttt acgtgaaagt agattattaa tgataatctt tatccaatta
      421 gaattattat cgtaggatgt gatgatgaaa gccgtgaaat tgttcaagta tttaccaatg
      481 tctccctgcc agtaaatccg cttagaattt gagccttgaa aatgtgcaat gataaaaaac
      541 aaaaactcgc tcaaaataga cagtttgggg tgggaataga ccaataatgt accagacaaa
      601 cttccatttg gggtgaaaat aggtcaataa agcgatgtta aacatacctt tggagtaaga
      661 atagagggta ataagtgaat ttataaactt tttggtgact ggttgagtgg aaaatgtagg
      721 tacatttcaa tgcgaaaatg tattataacc ccaaacgagg tcaataaaaa tgactcaaac
      781 ttgcaatata accctaaacg ggtaggggat ttatgaaaaa ttgaatttgc cggcgtagga
      841 gctggctgtt ttaagtatga atgaacacag ctttcgggct gttttatata aatagcaggt
      901 tactgttcac aaacagcatt ttgttaagaa agtagtgacc acagcagtgg tggaaagagg
      961 aaatattatg aagaaacagt ataacattgc agtcgccggc accggttatg ttggtttgag
     1021 tattgccact ctgttggcac agcaccataa ggttacagcg gtggatatca ttccagagaa
     1081 ggttgaactg attaataacc gtaaatctcc aatccaggat gattatattg aaaagtacct
     1141 ggctgagaaa gaactgaatc tgaccgcaac attggatgca aaggcagcat accagtctgc
     1201 ggattttgtt gttattgcgg ctcctactaa ctacaatagc cgcactcaac attttgacac
     1261 atccgctgtt gaggctgtta ttaggctggt catggaagta aatcctgatg ccattatggt
     1321 tattaagtcc accattcctg ttggatacac cgcttctgtt cgtgtaaaga ctggtagcaa
     1381 gaatattatt ttcagccctg agttcctgcg tgaaagtaag gcactgtatg acaatctgta
     1441 tccttcgcgt attattgtgg gtaccgatat ggatgatgag cgtttggttg aggatgcaca
     1501 cacttttgct acactgctac aggaaggcgc aatcaaagag aatgtggaca cattgttcat
     1561 gggctttact gaagcagaag ctgttaagct gtttgccaat acatatctgg ctcttcgtgt
     1621 cagctacttc aatgagctgg acacttatgc agagatgaaa ggcttgaaca ctcagcagat
     1681 cattaatgga gtttgccttg atcctcgtat tggtactcat tacaacaacc cttcctttgg
     1741 ttacggtggc tattgcctgc cgaaggatac caagcagctt ctggccaact acgcagatgt
     1801 tcctgaaaat ctgatcgagg caatcgttga atctaaccgt actcgtaagg actttattgc
     1861 ggatcgtgta cttcagatgg ctggttatta caattacgca gaagcaaatc agtatgatgc
     1921 tggcaaagaa aagcctgttg tgattggtgt ttaccgtttg accatgaaat ccaattctga
     1981 taacttccgc cagagttcta tccagggcgt tatgaagcgt gtaaaagcta aaggtgcaac
     2041 tgttgtgatt tatgagccga ccttgaaaga tggagagact ttctttggca gtaaggttgt
     2101 gaatgatttg gaacagttta aggcaatgtg tgattctgtt atagcaaatc gctacgatgc
     2161 ttgccttgat gacattaagg aaaaagtata tacccgtgat atcttccaga gagactaaga
     2221 ttaggagaga gtatttatga ttattacaaa aacaccgttt cgtatgtctt tttttggcgg
     2281 tggaacggat atggaggatt atttcaaaga gaatggcggt gcagttcttt ctactacttt
     2341 tgataagtac tgttatgtga atgtgagaca tctgcctaga ttttttgatt acagcgctga
     2401 gttatcttat tctcgtactg agagagtaac tagcattgaa gatattgagc atcctgcgat
     2461 tcgtaatgcg atgaaaatgc tcgatgtgca tgaaattcgg cttacatacg aagctgattt
     2521 acctgctcgc tctggtctgg gtacatccag ttcttttgca gttggtatgt tgaatgcttt
     2581 ttatgcactg gagggtaagt atgctgacaa gaagaaactt gctgatgaag ccatttatct
     2641 tgaaagagtt ctgtgtaatg aagccggtgg atggcaggat cagattgccg catcttatgg
     2701 tgggttcaat cgaattaatt tcaatgtgga tggttatgag gtgctgcctg tcattatttc
     2761 gcctgaacgt aaaaagcagc ttaacaacaa tctgatgatg ttctttaccg gattcactag
     2821 attttcttct gatgttcaaa aggctaacaa cgtcagtggc acagaggata agagagtaag
     2881 actgaaaaag atgtatgagc ttgtagatga agctgaggca attcttactg ataagaacag
     2941 aaatttagat gatttcggta gacagcttga tgttacttgg agattgaaaa aaggtactgg
     3001 tggagcaatt agtacaggaa gcattgatga actatatgaa aagggtatgg ctgcaggtgc
     3061 tttaggcgga aagttgctcg gtgcaggtgg tggaggtttc ttggttttct atgtacaacc
     3121 tgagaaacag gatgcagttc gttgggctat gcgtgatttg atgtatattc cgtttgaatt
     3181 tgaagatggt ggaactagag ttattcacta cacgccggaa acttatattg aaaaacagaa
     3241 tggataagga gtaacaatgt tagcggaaaa acttaataaa catattgact tattgattaa
     3301 acgatatcct acacttgaga cgattaaaca ggacattatc gatggatacc taattatgga
     3361 ggaatgctat ttgaatggtg gtaaacttct tgtggcagga aacggaggat cagccgctga
     3421 ctcggaacat attgctggcg agttgatgaa gcggtttaag acgccacgtc ctgtaagtaa
     3481 ggaatttgcc gataagttag ttgcaattga ccctgtaaga ggtccggaat tagccaaaaa
     3541 tctcgagtgt ggtttaacgg caattccttt ggtcgcacac gaagcattgt ctactgctta
     3601 tattaatgat gtcgatggtt tgggtgtctt cgcacagcag ctgtttggct ttggtagatc
     3661 tggtgacgta ttccttggta tttcgacgtc cggaaatagc aagaatatta tgagcgcaac
     3721 tgttgttgct agggctctcg gtataaaagt tattggcttg acgggcgcaa agggtgggga
     3781 acttgccacg gtagcagatg tttgtgttaa ggctcctgaa gttgaaacat acatgattca
     3841 agagtatcac ttgccgatat atcattgttg gtgtttgatg ctggaagata aattctttgg
     3901 aaacgaataa aatttggagg aatgttaaat gaaaaagaca attatggtta ccggtggttg
     3961 cggtatgatt ggctccaatc ttgttaagag actggtgaag gaaggctgct gggatgttta
     4021 tgtagctgat aatctgtgga gaggaaagtt ggagtacctg aacgacgaag atgggcatcc
     4081 tgtcatcgac ctggatactc atttcttcaa tgctgatctg actgattatg agcaggctaa
     4141 aaaggttatt ggtacgactg agtatgtagt tcatctggct gatgttgtgg ctggcattga
     4201 ctatgtgttt aagaaccagg gcgaattgtt ccgaatcaat aatctgatca attctaatgt
     4261 ttttgattgt tgccgtaagg ctggcaagga gaaaatcaag ggtgttatct atgttggtac
     4321 cgtatgctcc ttccctctga ccagacagaa tactttgaat cctgagcctc tccgggaaga
     4381 ggagttgttc cctgctcttc ctgagtctgc ttatggctgg agcaaactga tgggccagtt
     4441 ggagatgggg tatttggaaa aagaaaccgg aattccgtgc tgtatcctga tgcttcataa
     4501 tgtttatggc acacctactg attttggcga acgtagtcag gttattcctg ctctgattcg
     4561 taaagcaatc aataatcctg aagagccttt taatgtgtgg ggtagcggag agcagggacg
     4621 tgctttcatt catgtcaatg atatcgtaaa tgcattggtg ctcgccctgg ataagggttg
     4681 gggtcatggt catattcaga ttggcccttc tcactgtact tccatcaagg aaattgcata
     4741 taagatcatt gaaatttctg gcaaagacat caagcctttc tttgatactt ctaagcctga
     4801 aggtgataag gctcgttgcg cagattattc taaggcgaaa gagattttgg attggaagcc
     4861 tactgtttct ttggaagagg gtcttcgtga atcctacaat tggattgaaa agcagattaa
     4921 gggttaataa gtatataagc tgttcttcct ccgtgtaatt agaggatgcg gaggaagaac
     4981 atatgagtaa ggatgtactg agatggaaaa gaatatgtat aaaagaaaac gtgatttcac
     5041 tgtgtgtgga atagtaggtg tttgtgctac tacaaagaac gagtttgtaa gggaagtttt
     5101 ttccatgcca acatctccgg taagtgcgac tgttgatctc gtgggagtgc cggccattat
     5161 ttcggcaaag gaaaatccca aaattgctga gatgtatagc caggctacaa tggctgcaat
     5221 agatggtatg cctattgtta aaattggacg gcgcaaagga tttacctgtg aacgatgtgc
     5281 tgcaccagat attatgggaa tggtttttga agaaggcgtt agacagggaa aaacacatta
     5341 tttctatggt ggaaaagata acgaagtgtt aaataagctt cgtgcaaacc tagaaagaga
     5401 ttacccggga atcaaaattg ttggaatgta ttccccacca tttcgtccgt taaatgaaga
     5461 agaggatagc aagatttgcg aggaaatcaa caacttaaat ccagattttc tgtgggttgg
     5521 tattggtgcg cctaagcaag aaatgtggat gtgggaacac cagaagaaga tccataacac
     5581 agtaatgttc ggtgtaggcg ccggctttaa cttttttgcg ggcactttgg aaaaagctcc
     5641 tgaatggatg gaaaaagcta gtctagagtg gttatttaga ctggtaaagg aacctcgtag
     5701 actatggagg cggtatatct tgggaggtat aaagtatatc tactatacta ttgaatctaa
     5761 gtaggggggc aataaaaata gatgaaaata cttatagtag ttacaatgac gaataacttt
     5821 ggtaagaagg ggtcctataa ttctcaggaa ataggattag ctcgagggtt agctgaatta
     5881 ggccacgaag ttattgtata taaaagtgta aaaagtgatg aggaagttgg aacagagcaa
     5941 ttaagcaata cggcgatgat tcactatatc aagtgtcgtt atgtaggatc aaatggattc
     6001 ttatcaacaa agaattttga taagaatgcg gatgttctaa tacaatttgc ggaccttcaa
     6061 ctatcagtac ctcatgtata ctcatggact gtaaaaaatg gaataaaata tataccttat
     6121 aatgggataa cgcgaagtca aagtggcaat aaatatataa agacaattat tgaaatattg
     6181 gcgcatcgaa attataaaat ttataagaaa aatgggtgtg ttgccaaaaa tgatcgagtt
     6241 tctgaaaaat tagtacaatt aggagtaaat aaagtgaaac ttgctcctgt ttgtgtggat
     6301 tttgaacagt tacataagaa ttacgaagca atagatttta ctactctcag agaaaaatgg
     6361 aatattcctc gcgatgttcg tgttatttta tatgtaggac ggtttgaaaa ggataaaaga
     6421 ccgttagata tgattagttt atttaataaa cttgatgagc ataattattt gctgatggta
     6481 ggaactggtg ctttactcga gcaaacgaag cagcagataa atttacttgg actgcagaat
     6541 agagtaaaaa taattgagcg tattcctaac aaagatattt gggagttata ttgcttatcg
     6601 gatgtatttg taaatctaaa ctatactgaa atttggggta tggttttgct agaggcaatg
     6661 tactatcgcc tcggaattat tgctgttaat gcacctggcc cacagaatat tattttggat
     6721 aatagatatg gaatgttggc taaatctgac gatgaaattt tgacattctt aaaagacgga
     6781 cgatttacca acgatatcat agatgcagca tatcagcgtg taactacaga atttagatgg
     6841 tcaaagtcgg gagaaattat gcttagcatg ttggaaggtg ataaatgata tggaagtaac
     6901 gaaattaatg gcattgagga atcgatatgc tttaaacata gttgataatt gcacgagaaa
     6961 aatagccaaa attcttggtt gttgtattgg taaaggtgca cagataggga actctgttga
     7021 atttgttcat aattctgtag gcactgtgat tcattctgac acaatccttg aagatggtgt
     7081 taaagtttat cagaatgtga cgtgtggttg agcagatagt aatgaacgta tcatcattcg
     7141 agtaaaaaag aacgctgttt tatgtgtggg tgctaaaatt ctttgtaaat ctaacacaga
     7201 attatgcgtt ggggaaggtg ctgtagttgc tgcaaataca gtggtattga ggtctattcc
     7261 accctacgaa gtgtgggggg tacctgctaa aaagatgggg agataaaaca tgaatgcata
     7321 taaattatac aaagtagaac gatggtttta cttacacaaa atggagtttt ttgccaagat
     7381 tgtaaaggca ctaatttatg tacttcataa tagctcaatt tcatatcaaa cagaaattgg
     7441 agataattgt aagttcttat acggtggcat aggttgtgta attggaaaag atacaaaaat
     7501 aggtaatcac gttataatcg gaacgaatgt actgactggt ggtagatcca ataagcctgg
     7561 tatgcctaca attggaaaca atgtatatat cgctactggc gcaaaaattt taggtaatat
     7621 cacaataggg gacaatgtaa tagtaggggc taatgctgtg gtcattaatg atgttgaacc
     7681 taattgttcg gtaggaggcg ttccggctag agtattgaaa aaagatattg atgtaaatga
     7741 ttattgtagt ttgaatcgtt actaaaagga gagattgtat gttgtatttt gttattcctt
     7801 taatgagtgc gaaggcagca aacaattggg agatggttag taccttattt aaccgaacat
     7861 tatggtcgtg ttataatcag actgatcctg atttcaaaat cattgttgct tgccatgaaa
     7921 ttcctaaact cacgcatgaa tatgatgaca gagttgaatt tattcaagtt agcgaaaagg
     7981 atgcccctat tcccacaaat caagacgaaa aaatgattga taagggttat aaaacacatg
     8041 ctctcgcaat gcgtttgcga gagttgggcg gcggtttcgc tatgatggtt gacgcagatg
     8101 atcttgtaag ttctcatttg gcggaatatg tgaattccca tccccaggaa aatggatttt
     8161 atgtcaaaac gggatacgtg tactttgttg gagacgatta tatgaaggtt ctaccaaagt
     8221 tctcatcagg aagtgcgtgt atagtaaaat atgcagtgga agacctgcct gaggcatatc
     8281 cggaagttat gacagcaaat tgtgatgata atccatggat cattagaaaa cgacatgggg
     8341 gcgttgttcc agcatgtgaa gagcaagggc gtccattaaa acctcttcca ttcaaagcag
     8401 cagtatatgt attaggaaca ggggacaatc actcgcttta tggtaaaacg acaaaatatc
     8461 agacgaagat gcgagagata ctggaaatgt ttatagcaaa acgtaaaatt aaaggaaaac
     8521 tgaaagcaga attttctgtt gattggttat aaggtgagta ttatgaaggt attgatgtgt
     8581 gaaagccaaa tgccagagaa ttggtattcg tttgatcttt ctaaagaact taatcaatat
     8641 gttgatttaa cattgctgtg taagcgtgat gttcctcctt tagatcctaa aattaagagc
     8701 aaacatattt tgtatcataa aagtacaaat aaagtgtttt cggcacttgt ttttctgaaa
     8761 agcttgttct taatttggaa ggaaataaga tttggtgatt atgatattgt tcatttgcaa
     8821 ttgatgaaaa gtctcaaata tgaaggcgga ttgtacgttt tatgtaagag acactataaa
     8881 aaattaatta tgacagttca taatgtgtta ccacatgagg ttcgtgaaaa ggatcgccaa
     8941 gagcacttta atatatattc aaattgtgat ggtttcattg ttcacaatcg agtaacagaa
     9001 cagcatttat atgctgaatt cccagaatta aatgcaaagt catatgtttg tccacatgct
     9061 atctatggag atgttaaaca tatatctaga gcgtatgata aagataagtt tgaatttatc
     9121 ttgtttggac aaattcgtcc atataaggga attgatgtac tgcttaatgc aatcacagaa
     9181 cttccaatgg atgtaagaaa gaaagttcat tttaccatag caggtcaaca acatcctaaa
     9241 caggatagta ctgactataa aaaaatgatt gaagatttaa aggtttcaga ttgtgttcta
     9301 tataagccag agagaatttc tgatgaagaa atggacagta tgttcagaaa tgcagatgcc
     9361 gcaatatttc catataaaga aatctatgga agtggagcgc ttcttatggc atacacttat
     9421 tgcttaccaa ctattgttag taatgtacct gcttttatgg aagaaaccga ggaaggaaag
     9481 atagggttac ttttcgaaaa tttgaattct aagagcctag cagacacaat tgtaaagtat
     9541 gtgaacctta gcccagctga gaaaagcaaa atagaggatg atatatatga gcttgtaaac
     9601 cgcaagtata cctgggcggc atctgcaaaa ctcacatacg aggcatacaa acaaattttg
     9661 aatgagtagt aaaacaatta gaaaggaata ataattaaca tgcagattag taaatggaaa
     9721 atcatacagt ttctaatggt ctttctaatg attatctcat caagcatatt gaagcctttg
     9781 ggcggctggt tgggagaaac gatgaacgtt agacttgtga cagtcattct agcaatagta
     9841 tgtttgttaa tagcctcatt accagcattt agaaaaaata gatatttaaa tttctgcact
     9901 ttactgattt gtgttgttat aatagcagct tacatctact cagataagac aatatgggtt
     9961 aaatctaggg aaagtatgag tttatactat gtattattgg cgtatccatt gttctgtact
    10021 ttaattaacc aagcgtggaa tttggattca atgctaaata cgatatgtgg tgtaacattt
    10081 ctttcataca ttcttagaac atctatttca cttattcaga aattttcagg agtacttctc
    10141 tatgaaaaca tatatcgtga gtgtgccccg gaaaattggt atcgtggagg ctttttaaga
    10201 attaatccac catgttttac aattataata ataccgattg ctttgtatat gattgagcga
    10261 caagttgtag ctagaagaaa agtaaaatat tatttactga ttgctagcgc actggcatat
    10321 tctgctgtta ttcatggttc tagatctgtc cttgtctatc aaattattgt acttggttgc
    10381 tatatccttt ttaagaaagg ttcttcgaaa agaaaaattg caatgtgggt tttagctggg
    10441 ttgcttgtag tcatatttat taattcgacc atgttctcaa catttgttga aactttcaca
    10501 aacagtacag gtgaatatgc aggatctgct gaatctcgat ttgcttcagt ttggtttttt
    10561 gttcctaaat ttttgcaatc gcctttgttc ggtactggta ttcttactgg tgaggaattt
    10621 acgtttatat taccaggagg agttagagga cacctatctg atattggatt tttctataca
    10681 attactcaat atggtgtagg tatacttata tttgatattc tgtttattat aaaagggttc
    10741 aaaacagcat tacttgcatc aaaagtgggt atgactggct atcaatattt agcatttgga
    10801 attacattaa gtgttttgct aacagggatt aatatagact ggttttatcc gatatttact
    10861 ccagctattc ctgtatgctt agcggttata gaatacatct atcaagaatg tagaagtgtg
    10921 gctaacatag aataggaaat attaatagga gtgcatctca tgaataagaa agagatttta
    10981 tataacatat tgaagaagta cgaaaattta ccatatgtgt ttaggaacat gtttggtcat
    11041 aatataagta aactcgtatc ttggaaattg actgctgatt gtgcaacagt agaagaaaat
    11101 tctgaagttt atgcagcgtt tcaattgcgc tcaaaggaca tgtctgatgt tccagtgatg
    11161 ggtttagtta attgttgcaa agatgtagca attgtaatgc aggggccact atgtttaaag
    11221 gatgatttct cttataatac tctggcctac tataagaaat gttatccaaa tgctttaatt
    11281 attctttcga catggaagga cgaagatatt aaggcattga aaaggataga ggatcttgga
    11341 gttatagtta tttgtagtga gaaacctgaa aagcctggac acttaaatat taattatcaa
    11401 gttggaaaca ctttgcctgg aatagtcaga gcaaagcagc ttggtgcaaa gtatgtttgt
    11461 aaaactagaa cagatcaaag aatataccat ccaaatgcaa tggcgttttt ctgcagttta
    11521 cttgaacagt atcctgttaa taacgaagat gattggaaat taatacaaaa tcagcgatta
    11581 ctgttactat caatgccgta tggagacatg tttttcccgt attgtttgtc agacttcctg
    11641 tattttggtg atgttgatga tatgataaac ctcttctcga ttcctagtga tgtgagagaa
    11701 aagggagctg tgtctagagg agtctcaaga agggagatat ctgaaaataa tttagctcca
    11761 gaagttcagc ttcttcgaag ctatatttca cgaatggggg gcaatgagga gtgctctatt
    11821 agggcatatt gggagtttgt aaaaaaccat gttattacta ttaacaaaaa tcaaattgat
    11881 ttgtactggc ataaatatgt aggtcgttat tcaaataata ctatttatgg tacgtattat
    11941 attgatgata gccaagatgc acttcactgc tataattttg attttataaa ttggttgaat
    12001 ttgtacactg gaaaatacga atacaaagca aaatatgaaa aatatatgga ctttgtttgg
    12061 gaagagtaat taacttttat agtaaaaagt actttaggga agggcatatt atgaaaacag
    12121 taattatggc gggcggacga ggaagccgaa tttcggaatt gtttccggat attcctaagc
    12181 caatgattcc tatagatggc attccagttt tagaacgaga attgtgttcg cttagagatc
    12241 aaggctttaa tgatattatt ttgactgttt cctatttgca tgaaaaaata gaggagcatt
    12301 tcggagatgg ttcaaaatta ggaattaaaa tcaaatattt tattgaaaaa actccacttg
    12361 gcaatgcagg tgcattgtat tttttgttgg atgaactggg tgatgaaccg tttttacttt
    12421 tgaatgctga tgcagtattt gatgttgatt tcaggcagat gattagattt catgttgaga
    12481 ataacgcaga tgttactttg tttacgcatc ctaactcaca tccgtatgat agtggattaa
    12541 ttatatccga tgacaataag aaagtgcttc aatggttagc gaaggaagat caaagacctg
    12601 aatattataa aaaccgagta aatgcaggtc ttcatgttat aaatccgaag gtgttgagag
    12661 aaaatccatt aatggaaatt gtaaataaat caatagttga aggtgggggg ctaccataca
    12721 aaattgattt agatagaatg attctgaaac cattggctaa cacagggcgt atgtattgct
    12781 acgattctcc agagtatgtt aaagatatgg gaacaccgga ccgatataga gctgtaatta
    12841 aggactttca aaagggaatt attgttggta aaaatctatc caatccacaa aaagcaatct
    12901 ttttagatag agatggaacg attaataaat acgttggttt tttgagaaat gcagaagaat
    12961 ttgagttaat tgatggagta tcggaggcaa ttaagattat taattctcta ggttttctgt
    13021 gtattgttat aacaaatcag cctgtaattg caagaggtga agttactgtt gatgagctta
    13081 atagtattca caataagctt gaaacaatgc tagggcatgc tggtggattt ttaaacggta
    13141 tttattactg tccgcaccat ccccataaag gatatgcagg agaggttata gaattaaaaa
    13201 ttgattgcaa ttgtcgcaag ccaaaacctg gactgttatt agaagcatca aaggatttta
    13261 acataaacct aagtcaatca tttatggttg gagatagtga aagtgatgta attgctggaa
    13321 aaaatgctgg ttgtaagaca gtactgatta cagaagacat caccttgcct gctaattatg
    13381 gccaagacta tagtgtctgt tcattgttag aatttgttaa aaatcattta tgcactatgc
    13441 aataattggt ttagtgaaac ttgtaaaagg agtaattaga tgaaggtatt aattccagca
    13501 gcaaaagttg ttgaaaaaga attgcagagt ataggaatgt taccagcaat tatctatcca
    13561 attaataaaa aaattgtctt tgattatttg tataatcagt ataatgcaat ctgcgattca
    13621 ttggatatta tctgttatga aaaagcggat aaggttcata gaagactgaa aaactatcat
    13681 gattccagaa ttcatatatg tgaccttcct gaattagggg atctagggca aaccgtgtat
    13741 tttgggttat cagatgaaga tgagcctgtt ctaattaact tcgcagatac aattgttatg
    13801 gagagtatgt ctgatataga aatggataca ttctattatt ctgaagattt tgtgtcgagt
    13861 acttggacat actttaaaat taatgaagga ataattactg atatccacga taaagaggaa
    13921 gcttacgtat ccgataaacg tgaaaaatta tttattggcg tatttatgtt ttcttcgtca
    13981 aaacttctga agagttgctt aagagaggca gttgagagtc ggaaacaggg gggaataaac
    14041 tcattttatc gggctttaca gctatacagc gaacgttgtc cgctaaaggc cgtaagaaca
    14101 gaaaagtggt ttgatattgg acacatcgat aaatattaca atacaaagtt ggaagttaaa
    14161 gcacgagagt ttaatcatat ctctatagat aaagatcgag ggatattgaa gaaaactagc
    14221 gatgacaagg ataaatttat tggagaaata aagtggtatt taaaacttcc tgctgatatt
    14281 gaatatgtaa gacctagaat ttttgattat tctacatctt acgtagctcc ctatgttgat
    14341 atggaatatt atgcatatca tacggttcat gagttatact tatatggtga tttaaaatat
    14401 aaccagtggc atgatctgtt tagccgtata aagtttatct gtagcgattt taagcgatac
    14461 accttaaaag atgaaaagat tagagagtca ttggaagata tgtatttgcg aaaaaccatg
    14521 cagagatttc aaaaattgtc tgaagataaa agattttcag ctttttttga gaaacaaatt
    14581 caaattaatg gtcgtaagtt tatttcttta accggtgtaa ttgaacttct aaagaatctt
    14641 atcccaagta tgctctatga tgtaaatgaa ttttgtatta tacatggaga tttgtgtttt
    14701 gctaatatga tggtagattc caattgttcc tttgttaagg ttattgatcc taggggaaaa
    14761 tttggaaaat atgatattta tggggacagc agatatgaat tagcaaaact atttcatagt
    14821 ttagatggta aatatgatta tatcatcaag gatctattta ctgtagaata tgataccgag
    14881 tgtgcagtga tagattatac aattaatacg cgtgtaagag attacgattt atttgaattg
    14941 tttaagtcgg tcttttcgga cgagattaag gacgattttg ataagattgt attgatagaa
    15001 gcgttgctat ttcttagtat gattccatta cacggtgaaa gttttgatca ccaaatagtg
    15061 atgcttgcta caggtatgga acttcttgca agagtaagta ataaagttat actctaatat
    15121 aaattgtgcc ttaatgaata acggttacaa tactgtttta gcaatgtagg cattaaggca
    15181 cgattatatt aattaataca atagaaggga agagaaaaat gtatagtgaa tattcttttg
    15241 tgtttgatat cgatggcact ttgtgtccga taaagaagaa ggatgagcga tatgaggatt
    15301 tggtgccgta tgagaatatg gttgaaaaac tgagattcta taaggagaat ggtgctaaaa
    15361 ttacattgtt cacctcacgc aatgttaatt cctataaagg aaatttggga atgataaatg
    15421 ctaatactgc aaaagtgctc ttggcgtggt tggataaatg ggacattccg tatgatgaaa
    15481 tcctgtatgg taagccttgg ccggggcata aaggcttcta tgtagatgat cgaacggtta
    15541 gacctgatga gtttttgaga tgctctgtag atgaattaaa tgaaatctgc aacagttcaa
    15601 aaaaaagatt ctaaataagg aggttagttc tatggaaaaa aagatgacag ttatcattac
    15661 gatggcaggt ttgggaagtc gttttcgaaa aattggttat gcggttccta aatatatgat
    15721 taatgccaaa ggaagaactt tatttgattg ggccatggat agtttggttg gatacaataa
    15781 aaatgttgat aagtatattt ttgttgttcg tcaggaagac aacgcttcag cttttataaa
    15841 ggagcattgt gcttactttg gtattgaaaa cgtagaagtg attgaaatag actatatgac
    15901 agatggtcaa gcaactacat gtatgttggc tattccttat tgcaatcaag acaatgagat
    15961 tatggtttac aatattgata catacgtgga atcaaatgag atgaaatacg aagatatttg
    16021 tggagatgga catattccat gctttcatgc tgatggcgat cattggagtt ttgcaaagct
    16081 taacgatcga aatgaagtaa tagaagtccg cgaaaaagtt cgcatttctg ataattgtac
    16141 tcttggtgcg tattatttct catcggcaaa gctgtataaa gctttatatg aagagtatta
    16201 tgcagactcc tctaatatgg agaagaatga aaaatatata gcaccattat ataactttat
    16261 gataaaaaaa gggttaaagg tgactattag catagtagat gcgaaaaagg ttcatgtgct
    16321 tggtactcca gaagagctca atgtgttttt gaatcaagaa taaatagtgt ttaggagaaa
    16381 atagcagtga ccagtaataa tctaattgta aaaacattaa gcagatataa gaatactcca
    16441 cttcctgtta aggctgcatt atggtttatg gtatgtggtt ttttacagag aggggtttca
    16501 atgattacaa ccccaatttt tactagagtt ttgtctacag aacaatacgg tttatacagc
    16561 acttatctat cgtgggaatc cttattgctc atgattacat cgcttagttt gttcaaagcc
    16621 atgatgaatc tttatgtaaa atatgataat caagaacata ttctttcgac agtatccgga
    16681 ttgactttag tactgactat tttttggttt gttattgcta tttttattaa ggatatattg
    16741 gcagaaattt taggattgac accagttcta gttgtctgta tgttctcata ttttatattt
    16801 caggcagaaa tacaatgttg gtctttacac aaacgatata tatatgatta taagacttta
    16861 ttagtagcta cccttgcagt tactgttggc tcatctgtag ttggctgttt agcggttctg
    16921 tttatatctc ctaccgctga ggcgagagcc ttaatgaatg tcctagtgac tggtgttgtt
    16981 ggcgtggctg tgtatatatc ggtatttaga agatgtaagg attttaagga taatgaagtg
    17041 tggaatttcg cagtagtatt ttgtgtatcg ttgatgccac actatttatc tgaatttatt
    17101 ctgaatggtt ctgataaaat tatgataaat tacttatgtg gttcgtcaga tgttgctatt
    17161 tacagcgtgg catattctat tggtgctcta attaaccttg ttacaaattc tgtcaattcc
    17221 acttttgcac cttatcagta tcaaaaaatc aagtcaggag aatatgattt actatcaaaa
    17281 agagctaatg aagttcttgg tttggtagcg gctatgcttt ttgcattaat gctatttagt
    17341 aaagaaattg tgttgatatt tggtgggcag aaatatatcg acagtgttaa tttaattgta
    17401 cctatatgta tcggtattta tttcaactat atgtttcagc tgtttgctag agtacaggaa
    17461 tactttgaaa agaaactcac tgtcgttatt ccatcggttt tgtgtgcaat tatgaatatt
    17521 gtactaaatt atgtttttat aaagttatat gggtatcagg cggtagcata cacaactgta
    17581 ttctcatata tgtttttctg cgtcatccat tatatttttt atagaaaagt ttgcaaagaa
    17641 ctattggatg gaaaaaacat atatgatgtc agaggaatac ttttgatttc tgcaatcacg
    17701 atagctggtg gcatcctaat ttcaataatt aataaatttg atttcataaa atatttaatt
    17761 atagcaattg cttgtgtcgc tgcaattata tttagaaaac gattgctact tttagtaagg
    17821 agaattatga gcaaataaac atttgctcat agagagatag agaatgatag atattatttc
    17881 tgctataatg gtaaatgtat taacagcatt atatcagccg ttcttatttg ccgtattaac
    17941 agcgtatctg tttatgtact ggtatctatt tatagttgat aactctcgag atcctaaaaa
    18001 cttaaaagat tctgtattaa tctggttgag atattttaaa gcatctagta aatttcgcag
    18061 actctttttt cttgcgtttt attttacttt gatattattt aaaaccttgc ttaatcgaaa
    18121 tatgtggatg aacccgcttt caaacgttat tggacagtgg tcattatata ttatagataa
    18181 tgctacagga gagagcagat tgacaacaga atgcgtggag aacacgatgc tgtttatccc
    18241 tttttgcatg ttattgttat ggtggcgtga cattaaatgt gatattatac gcaccatcta
    18301 tgttgggatt aagtatgtgt ttttattctc attaagtata gaatttacac aactgttttt
    18361 taggcttggt acatttcaac tatcggattt gttttataac accctagggg gcttaattgg
    18421 tgctttgcta tattggctat tctaccgatt aaataagtat atagacttaa aatagaactg
    18481 ctttgaattt taatatggtt aatatttttc tgcagacagg attgtgtaat ctcatcaaga
    18541 atactttaca tgccatctat ttttgctaaa acattgttgt tatatcttca agaggtaggg
    18601 aattttattg ctttaaatga ggtcgagcga attaaaaaat tggtaaaata tataacatgg
    18661 tggtagttat ggtaaaagtg tttgatataa ttaagttttc ggatttcaga ggatatactg
    18721 ctgttgcatt ggataatgag actcttttct cgattggtat tgagtttcac attaagcaaa
    18781 taaatcaagg atttagtcat aataaatata ctatgcgagg gctacattat cagagtggag
    18841 agcatgctca aggaaagata gtgatgtgcc tacatggtga aattatgaat atcgcacttg
    18901 atattcgaca aggatcttcc acttatggat gtgtgtatgt caacacattg agtgaaagca
    18961 atggtaaatg tgcgtatatc ccacgtggat atgctcatgg ctatcttact atggaggaca
    19021 atacgctagt tcagtggtgc gtagataatg atttcaattc taatgctgcc aaatgtataa
    19081 gttatgaaag cgtagatatt gaatggccgg tcagtcggca gtgtttcgtt ataagtgaga
    19141 aagacaaaaa tggggaaaga ttaagctttt gattaaaatg agctgtggag aaaagaagga
    19201 tactggaaaa taagaagaac aataaccgag caaatgctcg gttatttcta tgaggaatac
    19261 ataaaactaa atttagatat agatatttat tcacgaaaaa ctaatatt
//
LOCUS       DACEPD010000056        18895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.057, whole genome shotgun sequence.
ACCESSION   DACEPD010000056 DACEPD010000000
VERSION     DACEPD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.057"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(21..251)
                     /locus_tag="IW797_001824"
     CDS             complement(21..251)
                     /locus_tag="IW797_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224605.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="IW797_001825"
     CDS             complement(687..1253)
                     /locus_tag="IW797_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1224606.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="IW797_001826"
     CDS             complement(1253..2209)
                     /locus_tag="IW797_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ1224607.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="IW797_001827"
     CDS             complement(2209..2865)
                     /locus_tag="IW797_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1224608.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="IW797_001828"
     CDS             3235..5253
                     /locus_tag="IW797_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1224609.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5680..8094
                     /locus_tag="IW797_001829"
     CDS             5680..8094
                     /locus_tag="IW797_001829"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ1224610.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="IW797_001830"
     CDS             8442..9995
                     /locus_tag="IW797_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ1224611.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="IW797_001831"
     CDS             complement(10376..12160)
                     /locus_tag="IW797_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224612.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="IW797_001832"
     CDS             complement(12175..13896)
                     /locus_tag="IW797_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224613.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="IW797_001833"
     CDS             13996..14757
                     /locus_tag="IW797_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1224614.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="IW797_001834"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="IW797_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..>18895
                     /locus_tag="IW797_001835"
     CDS             16664..>18895
                     /locus_tag="IW797_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224615.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6205 a         3353 c         3786 g         5551 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
    18841 tcaaggagct gtttttgagt tacagaaccg agaaggagaa acgctacaaa ccggt
//
LOCUS       DACEPD010000057        18736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.058, whole genome shotgun sequence.
ACCESSION   DACEPD010000057 DACEPD010000000
VERSION     DACEPD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.058"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(48..1112)
                     /locus_tag="IW797_001836"
     CDS             complement(48..1112)
                     /locus_tag="IW797_001836"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ1224616.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(1109..2194)
                     /locus_tag="IW797_001837"
     CDS             complement(1109..2194)
                     /locus_tag="IW797_001837"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ1224617.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(2207..3184)
                     /gene="mvaD"
                     /locus_tag="IW797_001838"
     CDS             complement(2207..3184)
                     /gene="mvaD"
                     /locus_tag="IW797_001838"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ1224618.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(3177..4121)
                     /gene="mvk"
                     /locus_tag="IW797_001839"
     CDS             complement(3177..4121)
                     /gene="mvk"
                     /locus_tag="IW797_001839"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ1224619.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            4443..5096
                     /locus_tag="IW797_001840"
     CDS             4443..5096
                     /locus_tag="IW797_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ1224620.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            5181..6086
                     /locus_tag="IW797_001841"
     CDS             5181..6086
                     /locus_tag="IW797_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1224621.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(6088..6720)
                     /locus_tag="IW797_001842"
     CDS             complement(6088..6720)
                     /locus_tag="IW797_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1224622.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            7040..7564
                     /gene="infC"
                     /locus_tag="IW797_001843"
     CDS             7040..7564
                     /gene="infC"
                     /locus_tag="IW797_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ1224623.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            7636..7836
                     /gene="rpmI"
                     /locus_tag="IW797_001844"
     CDS             7636..7836
                     /gene="rpmI"
                     /locus_tag="IW797_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ1224624.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            7889..8248
                     /gene="rplT"
                     /locus_tag="IW797_001845"
     CDS             7889..8248
                     /gene="rplT"
                     /locus_tag="IW797_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ1224625.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            8600..9838
                     /locus_tag="IW797_001846"
     CDS             8600..9838
                     /locus_tag="IW797_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ1224626.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAES
                     YTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLL
                     PVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGF
                     LMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSA
                     ILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIP
                     TGWIIGAILCLITFLLYGNA"
     gene            9886..12015
                     /locus_tag="IW797_001847"
     CDS             9886..12015
                     /locus_tag="IW797_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ1224627.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            11990..12679
                     /locus_tag="IW797_001848"
     CDS             11990..12679
                     /locus_tag="IW797_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224628.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(13087..13773)
                     /locus_tag="IW797_001849"
     CDS             complement(13087..13773)
                     /locus_tag="IW797_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1224629.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13909..14103)
                     /locus_tag="IW797_001850"
     CDS             complement(13909..14103)
                     /locus_tag="IW797_001850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224630.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(14153..14593)
                     /locus_tag="IW797_001851"
     CDS             complement(14153..14593)
                     /locus_tag="IW797_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ1224631.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(14597..15442)
                     /locus_tag="IW797_001852"
     CDS             complement(14597..15442)
                     /locus_tag="IW797_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ1224632.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            15591..17009
                     /gene="pepV"
                     /locus_tag="IW797_001853"
     CDS             15591..17009
                     /gene="pepV"
                     /locus_tag="IW797_001853"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ1224633.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(17085..18287)
                     /locus_tag="IW797_001854"
     CDS             complement(17085..18287)
                     /locus_tag="IW797_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224634.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(18274..>18736)
                     /locus_tag="IW797_001855"
     CDS             complement(18274..>18736)
                     /locus_tag="IW797_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224635.1"
                     /translation="NLPSNIKIYTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMK
                     QVDLIEHLTGLSGMKKIIDARDFFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLV
                     FATIMIATPVAFSLRYVFVLALGLPVYILSPFLLDKREKMKTDIGDTHETI"
BASE COUNT         5765 a         3555 c         3866 g         5550 t
ORIGIN      
        1 tgcttccgcc gtttatacgt gtttagagcg tgggataaaa gtgttcttta cttttgtccc
       61 acgctctttt ctgtgcgtct tccatacttc actaagtcga tccctcttgc ttcgcaccaa
      121 tccttcactg gtccggaaaa gatcagtgat tgttggtgta gggccgcggt attcgttgcg
      181 ccgaccatag tataaagcag acgaagctct tcttgccatt gcttcatcag tataatagtt
      241 tcttctacgc cgtgattcat cagatgcgtc agtacggttc ctgaagtacc cactgcattt
      301 gcgccgagac aaagggcttt aaatatgtca taggcattgc ggatacctcc ggaggctagg
      361 atttccatag aagtgtctgc ttcatttgct tcaagcaatg aagagactgt cgattgcccc
      421 cagtcagcta aataactcag ttcccgcttg gagcgacggg cgttttcgat ctgggtgaag
      481 cttgttccgc ttcgaccgct gatatcaact gtatgaactc ctaaagcagc tagatcattg
      541 agcgtttctc ttgtcatgcc aaaaccgact tcttttacga tgagggggac gtcaatggct
      601 gtttgagtct cttgaataag tactttccaa ttggtaaaat cacggtctcc ctcaggcata
      661 accaactctt gaggtgcatt caaatgaatc tgtaaggcat ctgcgtgaaa caatcggata
      721 gcttcttggg ctctttcgac agaagtccct gcaccgatgt tggcaatgat tttcccgtga
      781 ggatattctt gtcgcataat cgtataagta tcggctaaag aagggtcttt caaggcagcg
      841 ctgactgaac cagtggcaat catcaaatct gcttctcttg cgacgattgc aagatcttgg
      901 ttgatttttt ttgttttttc gcttccgcca gtcatcgcat tgatataaaa tgggctgctt
      961 agtgtaaacc caactgcctg tgtacttaga tcgacatcag aaactgctat ttgcggcaaa
     1021 gggttatgga tgatccgtac gaagtcaaat tcgttttttt gtttatcatg aaaggcttta
     1081 gctagtgaaa catgttcgtc ttttcgattc atcggttttc ctttctttga tcgctataga
     1141 catgtaacgg cagtggagtg atttctgctt tttcccatgc actcattaaa ggaaggatgc
     1201 cagatttctg gtcaacaatt acgattccgc aatcgccacc acctgcacca gaagattttg
     1261 cggctccttc atactgttca gctaaattac acaatttgtt caaagcaggc gtttcgatga
     1321 cgaccccagt gattgcagat aaatcatgca gtaattgtcg gttttttcga atcatctgtt
     1381 ggatcaacgt tacattattt tctttaaacc ctttgatcat ttcgttgaca cattctgtac
     1441 tgttttttaa gaaaagctga taagccacca ttttgtcttc tctcgaacgg tgaacttgat
     1501 cgaccaaatc agaagtagag gcagggctac ccgtccaacc aatcaataaa cgtaaatctt
     1561 caggagcaat caatggctca atggatagac ctggccaatc cagggcaagt aactcactga
     1621 tagaatgctg agtttcttgt tcttggagcc aaggatggtc gaaggttgag aaagcgatcc
     1681 agccaccata acagctagca gcgatgtcgc cgcaggaacc attatcttga actgctaaat
     1741 tggctagtgc tgcaatcttg aaaatctcca attgagataa attcaacgcg taaaaaacat
     1801 tcaaggcttt gaccgttgca acggtcacgg caccgctaga acccaatcca tatttccggc
     1861 catttgaact gtctaactca cttgttactt tcagatcata aaaggataaa agaatgtttt
     1921 tttcttgtgc atacttttca gtcaaacgaa tagcagcaag gatataatga aaaggatttt
     1981 cccgaatatc taaaactaat tctccgttgc gtcttgtcca acgtacaggc atcccactat
     2041 attgtgcaga ttggatactt ccgacttttc gtgcggattc tacagtgact gttacgaatt
     2101 gatcgactgc agcgataact gctggatgtc ctgtttcaac aactgcatat tctccggcaa
     2161 tatagagttt gcctggtgca gatacttcaa tcataaaaat ctccctttat tcaataatcg
     2221 caattcctgg accagcatgg gcagtaatta cttgttcttt gctaaataat ttggtaaatg
     2281 tttcttgtac ctcagataaa tggctgtttt ccactaaaac tttgacatta gggccagcat
     2341 ccatcgtaaa gtagcatgga atgccttttt ttctcaattg ccgcaccgca tccatcgctt
     2401 ttaagctgtc aggagaccag taagtgaatg gaggctgggc agctaaagtc gttccatgca
     2461 tttttagtcc gtttctttcc atcgtttctc cgagaagttg gaaatctttt gttttgattg
     2521 cttgtttcaa ttgatataga tccccttcaa cggaatctaa ccagccttga taaaaactgg
     2581 atgtttcgac tgttcgacgc atcccatttc tgctggacac ttcttttttc tggtcgttga
     2641 tcaaaacgaa aaccattgct aaatcgtctt cgaaagaatc ggaagggact ggcttagcgt
     2701 aagaacttaa gtcgtcatgg cctttttccc attcaacgaa accaccgaaa atacttcggc
     2761 aagctgaacc agacccgcgt cgagcaaatc ttgaaagaga gagatcgtct aatccaagct
     2821 taagagcagt attgcaagct cctgctaaag cagctagccc gctggcagag gaggctaatc
     2881 cagctgcggt aggaacgaaa ttctgactga ttactgaagc ttttagtgag aggccggctt
     2941 ctttacgaaa aaggtcaaga aactgactga ctttttttgt tgctttttcg tcttgtaagg
     3001 tgccatctag ataaaattca tctaccatat agctatcaga aaagatgact tctgtctctg
     3061 tataaaatgc atccaatgtt aacgaaaggc tgttgttcat tggaaggatg agttcttcat
     3121 ttttcttacc ccaatatttg attagagcaa tgttggtata tgcgcgtgct ttgcctttaa
     3181 acataggtat gtactcctaa tccttgtatc caagtttcag cagcacccgc atcttcaagt
     3241 gcttgactga tttcttgggc ttttgttttt gtttgtgcta atgcaatcat acagccaccg
     3301 cgtccaccgc cagttaattt ggcacccaat gccccggtat cttttgctgt ttggatcaat
     3361 agattaagaa aatcattgct gattgtcagc ttttccagaa gtgactgtga ttcatccatt
     3421 gtctgtgcca acgtttctgg tgaattctca atgattgctt gttttgcttg cttcgtcaag
     3481 tatcctaatt tttggatcat ttgtccagtt tcatgaggct gggtttcaaa gaggtgagcg
     3541 acatctttga cggcttctct tgtttgtcct ttgattcctg tatcagcgac aatcaaaaaa
     3601 gcatcgatgt tcaaagaaaa gtagtcaaaa ggatgccctt ttgtaaaata aatcggctgc
     3661 aagctgctag tagcggctgc atcgattcca ctaggattac cgtgggcgat tttttccgaa
     3721 agctggacat tttctaatag tatttcacga gacaaaggaa atgctaagta atcataaaaa
     3781 gcacgagtga ctgctgtggc gactgcagcg cttgatccca ttccacgttc agcaggaatc
     3841 gtgctttcaa tggtcagttg caagtttttt tggatgtcat gctgtttttt taagtgaatg
     3901 aataattttt tgatattttt taatgcatga ggagcttcgg ctaaatttcc tgaatagtag
     3961 gaagaaacca gttgatcgtc cattgcatcc agctcttcaa ggaatgcggt gacttttgtt
     4021 gcatagaaag gaaaggcaat cgctggttct ccataaacaa ccgcgtgctc gcccatcaat
     4081 atgatctttc cgcttgattc tccttggcca tagtttgcca taatctaact ccttctcacg
     4141 taaaagtgat ttttaagcaa aaaataaagc gcccaaaacg agttgcgctg cttcacgaat
     4201 ttttttgtcc actaagggtc tcaaaaaaca ttcctccttt attttactaa aaccatctca
     4261 gaagtaaaac taagaatacg ttttctaaag aaaaaacact tttttcttaa gaattttttg
     4321 actttttttc ttctttgact agaaaaaata caattgacac tactagatat tgggggtata
     4381 tttaaaaaag gttggagtta aacacaatat ttagtgatga agtgagctgg aggtaaatga
     4441 gcgtgatcgt tttagcaggt acaattggtg ccggaaaatc aagtttaaca gaaatgatgg
     4501 ctgaacactt tgatagtcag gcattttatg aatcgattga tgataatgaa gtcttgccct
     4561 tattttatgc aaaccctgaa cagtatgctt ttttgctgca aatttatttt ttgaataaac
     4621 gttttgcaag catcaaacaa gcaatgaaag atgacaataa tgtattggac cgttcgattt
     4681 atgaagattc actgcttttc catctaaatg cagatctagg aagagcaaca gaaacagaag
     4741 ttcgtgtgta tgatgaactg ctggaaaaca tgatggaaga acttccctat gctgcccata
     4801 aaaaacatcc ggatttatta gttcatattc gtgtatcttt tgatacgatg ttagagcgta
     4861 tcgaaaaacg tggtcgttcg tatgaacaat tgtcctttga cccaagtcta tacgattatt
     4921 acaaagaatt gaacagacgt tatgatcaat ggtatgaaga atacaaagaa agtccaaaaa
     4981 tccaaatcga tggagaccgc tataattttg tggaagatcc tcaagcaaaa gaagaagtct
     5041 tgaaaatgat cgaagaaaaa ttagcagaga ttcgccaaac caaagtatcc atttgattta
     5101 cagttgctca aataaagggt aaacctttat ttgagcgact tttttcttta tactggaaac
     5161 aaatgtcagg aggatagagt atgcgggaaa aacactattt taatcaagac cgtaattttt
     5221 ggatcaatgt acagcaaaac gatgtgctag ctgttaaaaa cttaaaagaa gaatatggca
     5281 ttagtgacga gatgctgact tattctctag ataaaaatga acgggcgcgc gtagagtacg
     5341 attcttttga tgaagcgtta ctgcttgtat caaatgttcc gcaccagcaa aaagtcgata
     5401 atcattatga aactagtcct atcgctttta ttttaaaaga agacggtttg tttacattta
     5461 ccacgccgaa taccgagtac gtgattcgac taattcgatc gcttttggaa cggatgccag
     5521 acatgagtgt atacagttta ttgtttcgta cattgttttt gatttctgat tccttttttc
     5581 ctttaatcga ggaagtgaat agtgaacgtc agcggcttaa tctgaaatta agagaaaaaa
     5641 caacgaacaa gaatctgctg caactttcag atttagagat cggtttggtt tacttagtta
     5701 caggaacaaa acaaaatgta gtacttttag aacaaatcaa ggcactggct atttatcgca
     5761 agttgtcaga aaaggaaaaa gaagagttgg acgatgcact gatcgaagca cgacaagcag
     5821 ttgaaatgac gaacttagct tcgcagattt tagaccagct ctctggcact tataataatt
     5881 tgctgaataa caacttgaat gacacgatga aatttttgac ggtctggtcg ctgatcttga
     5941 ccgttccaac tattgtgaca ggattcttcg gaatgaatct ccagttaccc tttacccatt
     6001 ccgttttcgg atgggaaatc gccttgatca tcagtttggt cttatctatt tggatgctta
     6061 ttgcactgtg gcggcggatt cgttgatcta gtctttgatc tgcaaaccaa gggcttgggc
     6121 gatcacaagt gcttgctctt gattgacgat tagtcccgcc aaagaatctt gagataaagc
     6181 gattgttgtg aagaaattag tcgtaaagtc taatcccttc agagaagttc gaaaccagtt
     6241 cgaaccggtt aactgattgt cagacaaaaa aaggtttttc caagtgacct cataaaattc
     6301 gctgtcttct aattgacaat gatcgaagga gacagctttc aagtttgtac tactaaagga
     6361 agccatcgaa gcaatacaat cagtgaaagt acagtctcga agataacttt cggcaaaatt
     6421 cgtacctgtt aatttacatt gatgaaacac gacttggtgg aaactgccac caatccactc
     6481 cagattggaa aaatcacatt tttcaaaaat gacattactg cattcaaaat gacgtaaacc
     6541 tgttttttgc atcgtaagtt tttggatatg actctctctt aataccatat ttttggcatc
     6601 gagatagctt tggttacctc cgttgatcag aagaccagag aagatctcct catcttctaa
     6661 ataaaactct ccctttgtaa gaagaggaag agaaggtgga ataggttcga tttttttcat
     6721 gtgggttcac ctcgtttttc tttagtatag caaagaagcg ggaatcactg caagaaagcc
     6781 tgctaatcta atcattttca gaaaaggaag acaaactgta tgaaaaacat tgacaaaaag
     6841 attagttttt gctatactat aatggttgag aaataaaagc agaagcaccc gcttctcgcc
     6901 ttagttgata atgtcactgg gctagatagt tttcactggt tctattaggt gaagtacgcg
     6961 cgggttctgt aagagaactc gtttttttat tgccgtttat tcggtaagct tttctttctc
     7021 tcataatatt tggaggtgaa tgaccatagc aaaggatatg atggttaacg acggcattcg
     7081 tgcacgagag ttacgattga tcggttcaga tggtgagcaa ttaggagtta agacaaaagc
     7141 agaagcattg aatattgcag aacaagcaaa cttggatctt gtgttagttg cccctggtgc
     7201 gaaaccacca gttgcgcgaa tcatggacta cggtaaatac cgtttcgaac aacaaaagaa
     7261 agaccgcgaa gcgcgtaaaa aacaaaaagt gatcaatgta aaagaagttc gcttaagtcc
     7321 tacgatcgac ttgaatgact tcaatacaaa acttcgtaat gcacgtaagt tcttggaaaa
     7381 gggcgataaa gtgaaagctt ctatccgttt caaaggccgt gccattaccc ataaagaaat
     7441 tggtcaaaaa gtcttgaatc gcttagctga agaaactgcg gatattgcaa cagtggaaca
     7501 aaaagcgaaa atggacggac gcagcatgtt cttgacgctg gcaccgaaaa acgacagtaa
     7561 gtaatttgaa tgactaactg gctgttggac agccgatagt cggataacga aagattttga
     7621 ggaggaaata tagtcatgcc aaaacaaaaa acacaccgcg gatcagcaaa acgtttcaaa
     7681 cgtactggta aaggcggatt gaaacgcttc cgcgcgttta caagtcaccg tttccacggc
     7741 aaaacaaaaa aacaacgccg tcaattgcgt aaagcaagca tggtttctag tggcgacttc
     7801 aaacgtattc gccaacaact agcaaaaatg aaataagaag tacaaataac agaatctgaa
     7861 gatttatttt tattaggagg aattatacat ggcacgtgtt aaaggtggaa cagtaactcg
     7921 taaacgtcgt aaaagagtgc taaaattagc aaaaggttac tacggttcaa aacacacttt
     7981 atttaaaaca gcaaaagaac aagtaatgaa ttcatacaac tacgcatatc gcgatcgtcg
     8041 tcaaaagaaa cgcgacttcc gtaaattgtg gattgctcgt atcaacgcag cagctcgtat
     8101 gaatggcttg agctactcaa aattaatgca cggtttgaaa gttgctgaaa tcgacatcaa
     8161 ccgtaaaatg ttagctgact tggctgttaa cgatgcagca gcatttactg cattggctga
     8221 ccaagcaaaa gaagctttgg ctaaataagc taaacaatta taaagaggaa agaagataac
     8281 gaaaagttat gttctttcct ctttttttat ttacaatcct ccatcaaatg aaacaggaca
     8341 ataacgtata agtctaaaat aacataatta aggtttttta attgaaaact gaacatttat
     8401 ggtcatcctt taatgaaagc gctaactaca taagttagaa tgagtttgac tgatatatta
     8461 gtttgcttat cagaaaaaca aaatggacgg aggaataaca tgagtgatat tattattggt
     8521 attttgttag ttatctcatt ctttttcatg gtctggtact gtgtaaaagg ctacaacttg
     8581 atggtcggct tttgtcatca tggctactgt atggatggga ttagcgctag tcggaaacac
     8641 gttttcacca aactcggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta
     8701 tacgacgggg ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt
     8761 tttcgggcgt gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga
     8821 acttggaggg gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat
     8881 attcatgtcg atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccgat
     8941 cttgatgtca ttaggtattt cagtaccggt tgcactgttc tcatttatgg gctcgattat
     9001 ggctggaatc tttgctaata tcgtcaattt caaacaatat caaacgattt atgctggttt
     9061 caatccagcg gctgaaagct atacttataa tgactatttc caaattggta tgattgggat
     9121 gattgtaagc ttagttgtgg tactaacggt cgctaatata tcaatgaaca aaaagaaacg
     9181 ctatgcaatg gcagcaaacg taccagctga aggtggcgac gcaccaatga tctcatggtt
     9241 agccgtatta ttaccagttt taggggtcgt tcttttagat cttcaaatca tcttaggttt
     9301 tattctagca ggtatctggg cattgctgtt tactggaaaa ttgcgcggag gttataaaga
     9361 aatctgtcgt cagtttgcga aattatttac agatggcgca gtcgatgttg caccaatggt
     9421 tggtttcttg atgaccttag cgatgttcaa taattcagct gcgtatgctt ctccttattt
     9481 ttcagctatt tttggagatt ggataccgca atcaccatta gtcttggcaa tcgtatttgc
     9541 tatattgaca ccactaggat ttttccgcgg tccaatgaat ctagtcggtt caggctcagc
     9601 tattttagct gtcgtgttag ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt
     9661 atttgcaatt acaacgattg ctcctcagca tttagatatt acacaatctt gggttgcttg
     9721 gggattaggt tatacaaaag taacttctcg agaatatatg aagaaatcca tcccaactgg
     9781 ttggatcatc ggtgcgatct tatgcctgat cacgttctta ttgtacggga acgcctagta
     9841 aacaaaacgt cgataaaaac gattagataa atggaggaaa agaaaatgaa acgttttgtg
     9901 cgaatgggaa ttgatgttgg cggtacacat acgaaagcag tcgcaatcga taatgcgacg
     9961 cacgaaatca tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg tggagtagcc
    10021 gcaggtgtcg ttcagtcttt tcaaaattgt ttgcgggaaa ataatatcag tccagaagat
    10081 gttgtgtttg ttgcacattc taccacacaa gcgactaatg cactgattga aggagatgta
    10141 gcaaaagtcg gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt agcaaaacga
    10201 caaactagat tgaacgatat cgatttaggg aataagaaaa aaatagagat tgtcaatgca
    10261 ttcttacctg tcaaaaactt aaatgtagac cgtgtttcag aaacgatttc ttctttagaa
    10321 agagaaagag cggaggtctt ggtttcttct atggcatttg gagtagataa tggggaacca
    10381 gaacgagttg tttatgaagc agcatctgtg aaagcgatcc ctacaacgat ggcttctgac
    10441 atcacaaaat tatatggatt gacaagaaga actcggacag cagcaatcaa cgcatctatt
    10501 ttaccaaaaa tgctagatac ggcaacctcg acagaagact ccgtacgaga agcaggggtc
    10561 aatgtttctt tgatgattat gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa
    10621 aaacgacctg ttttaaccat gctttccggt cctgctgcat cagtcatggg ctctttgatg
    10681 tacttgagag cgtctaatgg tgtttatttt gaagtaggcg gaacaacaac caatatcggg
    10741 gtcattaaaa atggacgtcc agcaatcgat tattcaattg taggtggcca cccaacatat
    10801 atctcttcat tagatgttcg ggttctaggt gtggcaggcg gttcgatggt acgggctaat
    10861 cagtcaggaa tcatcgatgt cggtccacgt tctgcacata ttgccggtct agattatgca
    10921 gtatttacgg agacagaaaa aatcaaaggt cctaaggttg aatttttctc tcctaaagaa
    10981 ggagaccctg ctgattacgt caaggtagtg atggaagacg gagaagaagt gactattaca
    11041 aatacatgtg cagccaatgt tcttggatta gtgcaagaag aacatttttc ttatggaaat
    11101 gtcccatctg cacgaaaagc catacaggca ttagcagact attgtcatac gacggttgaa
    11161 gacatcgcag agcagatcat ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa
    11221 ttggcagaca agtatcactt ggagaaagat cagatttctc ttgtaggtgt agggggcggc
    11281 gctgcctcat tgattactta ctttagtaat aaaatgggtg tgaaatattc gattcctgaa
    11341 aacgccgaag tgatttcttc tattggtgta gcacttgcaa tggtccgaga tgttgtagaa
    11401 cgtatcatcc cttcgccaag taaagaagat atacgatcgt tgaaaaatga agcgatgaat
    11461 aaagcgattg aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga aatcgatcca
    11521 catacttcaa aagtaacggc tatcgctact ggttctacgg aagtgaaagc aaccgatcta
    11581 accaaggaga tcacaacaga agaagcacta gagcttgctg cagaggatat gcgattaaat
    11641 aaaaatgaag tttgcttatt ggaaaataca cctttctttt atgtatgcgg cgaacagaat
    11701 cgttcaaaaa atgctggaag tcttcggatc atcgatcaaa aaggatttat caaggtccaa
    11761 cgtggacatg cttcatgtat gaaaacaact gcagccaatt atatgacagc tgtcgaacaa
    11821 ctttgggaag atatggcagt ttatcaaact gaattgattg cccgaccaga attttatctc
    11881 tgcttaggag caagagtctc tgattttaca gcgacggatc tggaacaact acagcttttg
    11941 atggatctgg aagtatctac tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat
    12001 atcaaacaaa cataagttag aagagttact aaacgaatta caacagatcc ccgaagaaat
    12061 ttggggattc taccagtttc ggcgagattt attttggaaa aagattcctt tgtccaagca
    12121 gaaaattctt atacaacaat caatcgattg tggtatcgaa acggcatgta gcatcaaaaa
    12181 aaagtaccct tttgccgatg taggagaaat ctatgaacaa atggctgttc caatcgtttc
    12241 ctgtgaatca gagcaaataa acgaacgaat cacttttgca acttatgcag aagatgaagg
    12301 gatccgattg atgacggagc cgttagaaaa attaaaatgt tcaggactag cttcgatctc
    12361 taaagaaaca gcgcaagctt tgattattgg acatgaacta tttcaccaca tcgaagcttc
    12421 tgttaaggga atttatacgc aaagcgaaaa aattgtgctg tggcggttgc ctttttatac
    12481 acatcaatca aacattcgtg cattgagcga gatagcagcg atgagttttt caaaagagat
    12541 gaatcaaagc gatttttcgc cttatgtatt agaggcagtc ttactttggc catataatga
    12601 aattcgtagc caagggatct tagaagaaat aaaagaaata gaaaaaagat gtacggagta
    12661 tgatttagat tacaaataaa gagactgtga caaaagtcgt gtcacagtcc tttttttccg
    12721 aataaacggt gttctcctgt caactcctcg gtcacaagtc aaaatattca acaaacatga
    12781 gaagctgttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
    12841 ttggaaaaag gtgttcaaaa gcttgcatct gcggtaataa gaacagccaa gttaaaaaca
    12901 taagaagcta tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac
    12961 ctctcaataa acggtgtgca atcagctgac cctcggtatt agctcaaaaa aaacggcaaa
    13021 atatgaggaa ctattttgcc gtttatttct ctaatactca ggtcaaaacg ctgattccaa
    13081 aacctcttat ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt aattatgaat
    13141 cacgtattct tgctcctcga taatcatatg taaatgcatc gtgcaaagaa atccgacttc
    13201 atcaaagtta tgatcaatca ttgattgaag cattgtctga tgttcatcta atactcgacc
    13261 ccagtctaat tctgaaatcg tcaaacgtaa ccatctgaaa cggttcaagt gcgtactata
    13321 actttctagc caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa
    13381 atcagtatcc aaatcaaaaa aagctttttt attttgagcg atcactgcct gatcggtttg
    13441 ttctagtatt ttttctagtt tagcttgatc ttgcttggtc attttcgcac tgcattcttg
    13501 caggatcggt ttttccaact tttcccgcgt ataacgggct aatgaagcag atcggatatc
    13561 gatttttgaa acataagtgc cggactgtgg aatggttttt acaagctctt gattgcgcag
    13621 ttggatcagt gcttctcgaa tcggtgtacg cccgatagct agttgttctt ctaacacttt
    13681 atcggagatt ttacttccag gtgctaactc ggaataaatg atttgtttac gtatagattc
    13741 ataagcttgc tgttgcaaat tattgataac catttaacct tcctttctat gcagatataa
    13801 tctattataa cataaatcgc ttacaaatcg tgtgggcata aaaacacagt ctgcaaaaaa
    13861 agccctgata ggcatgccta ttagagcctc gaaaagaaaa atattgaatc aatctggtcg
    13921 tacctcttgg atcggtttga cacgaccgcc gattctttta gcaaaagctt cggattcttt
    13981 tttgtctttg aaaatgagca gattgtctaa gtttttttcc cgagtgcgac agccagtttt
    14041 atcgatgtaa ccgtgcatta ctttaacgac atacatgggg atcacctgct tttttctttt
    14101 cattatacga actactcgac acaatacaag aaaaatgctt agacggtatt tattaagaat
    14161 ttacatcatc caaagaagcg tacgcttgga ataactgttc taactccgga ttgactttgc
    14221 tcagcttcac taatcgttgc gaatcagaaa gtaaaaaaca gtgtttacta ctgccagtag
    14281 tttttaattt tttgtcttca gttcccacaa tttcgtagct aaaatccaac cgtgttcccg
    14341 tatacttttg gatagttggg tggatgctca ctttttctcc gtaatggatc atttctttat
    14401 agctgcaatt tacttccaga acggggatga tgatcccaga ttcttcgatt tttttatagg
    14461 gaaaccctag atgtaccaat aaggctaccc gagcctcttc aaaccagcgg atataattgc
    14521 tgtgatgaat aatccccatt tgatctgttt cataataaaa aggttctcgc acataacctg
    14581 taaatttctc catgactcat gcctcgtttt ccattgattt tttacaaaaa taattcatcc
    14641 acttcggaag tgcttcgctg atttgtgtcg gcaaaacaat atattgttct tttgccagct
    14701 ggtctccaat gaaactatga agataaacag ctgctgcaat cgtttggtca ttttgaggaa
    14761 actgggctaa aaatcctgtg atcatcccag ctaaagtgtc acccatgccg cctgttgcca
    14821 tagcagcaga acctaaagga ttggtgtaga agtgctggtg accataaatt tttgtctcat
    14881 gacttttcaa aacgagcaca gcaccgattt cctgttgctt ctttttgttt tctttatccc
    14941 gttgttcttg tatcggtaga gaactcaacc gttgccactc catttgatga ggtgtaagta
    15001 cagtttgttc gggatagggt agcaaaagat gattagcaac aagtaaactt aaagcagagc
    15061 catcaatcac cagccattgt tcttttgatt gtttttctag tacttgtttg aaacgttcca
    15121 aactcttaga agaagtaccc aatccgggac caatcaagag tacatctgct tgctttatta
    15181 cggaaagaaa ctggtcagta tccgtccaat ctacaatcat tgcttctggc agatgactgt
    15241 gaagagcact ccgatttttt tcatcagtta ctacagtcgt gagacccgct ccactgtgta
    15301 cggcagctaa agcactcata atgattgctc cgccatattg ttcattgccg ccaatcaaaa
    15361 cgatacgtcc aaatgttcct ttatgtgaaa tctttggacg aggtgtgatg actcttttca
    15421 ctagttgttc gtttatcagc atcgaaaaac ctccttttac tcattatagc gtatattttt
    15481 tttagagaaa aacagataag aaaaggaatt gtcagatttt ttgaactatt tttttctgta
    15541 atttgtggta tcatgaacac gaagacttaa ataagtggag gattaacttt atgacaattg
    15601 attggcaaaa agaagtagaa gcacgtaaag aagacttgct tgaagacttg aaaaatcttt
    15661 tacgtgtcaa cagcgagcgc gatgattcaa aagtaacacc agatgcacca tttggacctg
    15721 gacctcgcga cgcattgaaa catatgttag cttatggcga acgtgatgga tttaaagtaa
    15781 aaaatgtcga taactatgca gggcatattg atcttggcga aggagacgaa acgctaggga
    15841 tatttggaca catggatgtc gttcctgcag gagatggctg ggacacagat ccttatgaac
    15901 cagtaatcaa ggatggaaag atctttgcac gcggatcaag cgatgacaaa ggtccaagta
    15961 tggcagctta ctatgcgatg aagatcatta aagaacttga cttgaaacta tcaaaaaaag
    16021 ttcgcttcgt agtcggcagc gatgaagaaa gcggctgggg cgatatggct tattatttcg
    16081 aacatgaaga agaaccagat tttggtttct caccagatgc tgaattccca atcatcaatg
    16141 gtgaaaaagg aaatgtttct ttagctttac gcttcaaagg cgacaatgca ggagattatg
    16201 tcttgaaatc ttttgtttcc ggacttcgcg aaaatatggt gccaggaaca gcaactgcag
    16261 cacttcaagt accaagtgca gatgcagcta tcgcgatgga agaagcattt taccaattca
    16321 tcgaagcaaa tccagtaagc ggaacgattg aagcggacaa tacgtatgta aaaatcgaat
    16381 tagttggtaa aggcgcacat ggtgcaagcc cacaatctgg tatcaatgca ggttctttct
    16441 tggcattgtt cttagataac tatgaattcc taggatctgc taaacaattt atccatgtag
    16501 cagctgctta tgtccatgaa gatttttatg gagaaaaact aggagtcgct tatgaagatg
    16561 aaaaaatggg caaattgaca atgaatgctg gactatttgc ttttgaagaa aatggtacag
    16621 aggaagcgaa cttcatcaat atgaatttcc gttttccaaa aggtgtaaca gtagacggat
    16681 tgcaaagtga catcgaacaa acagtggggc aagaaggcgc aacagttact cgaggtgctc
    16741 gcgtaatgga accacattac gtaccaatgg atgatccatt ggtggctaca ttattgcaag
    16801 tctatgaaga ccacactgga gaaaaaggct acgaacaaat catcggtggc ggaacttacg
    16861 gacgtttgtt gaaacgtggc gttgcttatg gagcgatgtt cccaggatat actgatacaa
    16921 tgcatcaagc aaacgaattt atgagtcttg atgacttgtt cagagcaaca gcaatctatg
    16981 cagatgccat ctatcgtttg gcgaaataaa aatactaaaa aactccggct atctctgaca
    17041 gctggagttt ttatgtttaa aaatgaatta gtacatcggt attcttaaaa gtaatttagt
    17101 tcgtcttttg atttctcagg agggatcaaa aaggcgaaag cactaagtat agcaaaactt
    17161 gtgatgattt gcgtacgata cgtaaaccaa taatgaatct gtgagtgatt agctaaaata
    17221 ttgtaccaaa tatacggcgt tacagcagct aaaacaaaca aaactagaag ttggaacgga
    17281 cgccagctat ttcgtttacg ataagcaaaa tagagaaaaa caagacaagt aatcaatagc
    17341 aataatattg ctacttttgg atacatcaat ctaatattgc ttttcagcat ctctaatcga
    17401 tgcaacgggt atttgtcgtt tccttccgtt cgaaagagga tttggttgat tgcatctttt
    17461 acaatgtttt tgttcaatat gatcgatgat aggatccatt tagccaccca tgtcaaacca
    17521 tagcctaatc cccaaaagat accggtaaaa acaaaactgc ctacttgttt gctcaatgaa
    17581 ctgcgtttat ccattaaata cttatttgtt acataataaa taatgatcac aggtatcccc
    17641 caagagatga ccggtgttgt caacaagtca aaaaagttag ctaaagaacc aatgacaaag
    17701 aaatacaaga ataattgttg gttggtgttg gagagttgtg gttttgttaa aaatagtaat
    17761 acagacaata aagtcaaaat ccaaatattt gaaaattgca tactgaagaa aaaggttgca
    17821 gcatttccta tagcaagcga aatcaacaag gctgtcgtta aatagatgtc gctacacttg
    17881 gttaaaaagt agctcgtcaa tccaatcagc agaaaaagaa tggctgcgta aatcattcga
    17941 atactcccca aactcatccc tagcagtaaa ggtcgtaaaa aaagctgata accatgccaa
    18001 taacggggat aatccgccat agacatcgcg ttctccaagg agttcaattc aggatctgct
    18061 tttgtttttc gtatcatcaa atgatcagta aaattatcta accgagtgcc tagcatattt
    18121 ccttgattga tagaaggata cattccttct ttttcaacca tctgtagaga tttttctgca
    18181 ttcgattcaa tagagtgctg cggaatgaaa aaagtaattg tcagtagtac gcaatagcta
    18241 gcgatcaaga gaacaaaagc aaaaaatatc tttttaaata gtttcatgag tatctcctat
    18301 atctgttttc attttttctc ttttatctag taaaaaagga gagaggatat aaacaggtag
    18361 acctaatgct aaaacaaaca cataccgtaa actaaaagcc acaggagtag caatcataat
    18421 agtagcaaaa acaagcatac cgggaagaaa aaggagtaaa taccgatatt ttttcttaaa
    18481 gattgctaaa agtgcactgc cgatagctaa ccacagtaat gtgccggaac caaagaaatc
    18541 acgagcatcg atgatttttt tcattcctga caatccagta agatgttcaa tcaaatctac
    18601 ttgcttcata ccataattat tcgtgtttac acgagtatct aaaaagccgt aactattttt
    18661 tgtaccaggt acccagaacc cgaacgtttc taaaatataa gcatctgtat agatttttat
    18721 attacttggt aaattt
//
LOCUS       DACEPD010000058        18138 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.060, whole genome shotgun sequence.
ACCESSION   DACEPD010000058 DACEPD010000000
VERSION     DACEPD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.060"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(28..726)
                     /locus_tag="IW797_001856"
     CDS             complement(28..726)
                     /locus_tag="IW797_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ1224636.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="IW797_001857"
     CDS             complement(719..2071)
                     /locus_tag="IW797_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ1224637.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="IW797_001858"
     CDS             complement(2294..3694)
                     /locus_tag="IW797_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ1224638.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="IW797_001859"
     CDS             3855..4592
                     /locus_tag="IW797_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ1224639.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="IW797_001860"
     CDS             4717..5196
                     /locus_tag="IW797_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ1224640.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="IW797_001861"
     CDS             5193..5960
                     /locus_tag="IW797_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224641.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="IW797_001862"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="IW797_001862"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1224642.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6863..7465)
                     /locus_tag="IW797_001863"
     CDS             complement(6863..7465)
                     /locus_tag="IW797_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ1224643.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7571..8040
                     /locus_tag="IW797_001864"
                     /pseudo
     CDS             7571..8040
                     /locus_tag="IW797_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8146..8730
                     /locus_tag="IW797_001865"
     CDS             8146..8730
                     /locus_tag="IW797_001865"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ1224644.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8733..9806
                     /gene="prfA"
                     /locus_tag="IW797_001866"
     CDS             8733..9806
                     /gene="prfA"
                     /locus_tag="IW797_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ1224645.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9799..10638
                     /gene="prmC"
                     /locus_tag="IW797_001867"
     CDS             9799..10638
                     /gene="prmC"
                     /locus_tag="IW797_001867"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ1224646.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10631..10810
                     /locus_tag="IW797_001868"
     CDS             10631..10810
                     /locus_tag="IW797_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224647.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10832..11857
                     /locus_tag="IW797_001869"
     CDS             10832..11857
                     /locus_tag="IW797_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ1224648.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12032..13276
                     /locus_tag="IW797_001870"
     CDS             12032..13276
                     /locus_tag="IW797_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ1224649.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13429..14376
                     /locus_tag="IW797_001871"
     CDS             13429..14376
                     /locus_tag="IW797_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1224650.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14510..15139
                     /gene="upp"
                     /locus_tag="IW797_001872"
     CDS             14510..15139
                     /gene="upp"
                     /locus_tag="IW797_001872"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ1224651.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15278..15520)
                     /locus_tag="IW797_001873"
     CDS             complement(15278..15520)
                     /locus_tag="IW797_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224652.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15641..15713
                     /locus_tag="IW797_001874"
     tRNA            15641..15713
                     /locus_tag="IW797_001874"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15674..15676,aa:Thr,seq:cgt)
     gene            complement(15832..16470)
                     /locus_tag="IW797_001875"
     CDS             complement(15832..16470)
                     /locus_tag="IW797_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ1224653.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16593..17225)
                     /locus_tag="IW797_001876"
     CDS             complement(16593..17225)
                     /locus_tag="IW797_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ1224654.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17576..17875
                     /locus_tag="IW797_001877"
     CDS             17576..17875
                     /locus_tag="IW797_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ1224655.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6221 a         3162 c         3573 g         5182 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
     7081 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
     7141 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
     7201 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
     7261 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
     7321 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
     7381 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
     7441 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
     7501 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
     7561 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
     7621 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
     7681 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
     7741 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
     7801 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
     7861 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
     7921 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
     7981 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
     8041 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
     8101 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
     8161 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
     8221 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
     8281 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
     8341 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
     8401 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
     8461 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
     8521 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
     8581 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
     8641 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
     8701 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
     8761 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
     8821 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
     8881 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
     8941 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
     9001 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
     9061 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
     9121 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
     9181 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
     9241 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
     9301 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
     9361 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
     9421 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
     9481 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
     9541 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
     9601 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
     9661 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
     9721 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
     9781 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
     9841 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
     9901 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
     9961 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    10021 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    10081 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    10141 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    10201 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    10261 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    10321 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    10381 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    10441 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    10501 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    10561 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg
    10621 atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa
    10681 aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact
    10741 attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa
    10801 gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga
    10861 attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac
    10921 agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt
    10981 aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa
    11041 agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg
    11101 accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg
    11161 attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact
    11221 ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac
    11281 tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc
    11341 tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat
    11401 gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt
    11461 tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata
    11521 caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc
    11581 agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc
    11641 ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc
    11701 ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga
    11761 tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt
    11821 gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct
    11881 tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta
    11941 actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa
    12001 attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga
    12061 tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc
    12121 ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa
    12181 atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt
    12241 tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca
    12301 gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga
    12361 cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa
    12421 cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat
    12481 tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg
    12541 tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt
    12601 aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca
    12661 tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg
    12721 ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc
    12781 ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc
    12841 attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc
    12901 aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac
    12961 agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga
    13021 gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact
    13081 aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt
    13141 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
    13201 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
    13261 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
    13321 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    13381 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    13441 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    13501 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    13561 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    13621 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    13681 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    13741 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    13801 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    13861 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    13921 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    13981 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    14041 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    14101 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    14161 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    14221 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    14281 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    14341 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    14401 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    14461 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    14521 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    14581 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    14641 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    14701 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    14761 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    14821 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    14881 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    14941 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    15001 tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    15061 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    15121 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    15181 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
    15241 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
    15301 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
    15361 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
    15421 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
    15481 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
    15541 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
    15601 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
    15661 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
    15721 agaagacaga tatatcaagg atttttaatc cattgataca tctgtctttt tgtttaaagg
    15781 aaataaagta tgctttctta gcaatagagc atgtattatt tctataaaaa tttaggagaa
    15841 taaaacgact agcaatatag ctgtgaaatc aaatatcaca tgaactgccc aacttgtaat
    15901 gatcgagttg cttttaatgg cagcttggtt gaataacaat cttgccaccc cttgtatcaa
    15961 aaggatgtgt gctacagtgt gtaaaatatt tccattatca taagtactgt aatggataaa
    16021 tccaaagatg attgctgaca agaaactagc gacaggaatg ctgaacttac tagaaaaaat
    16081 agcgagaaaa tacactgaga acaattcttc tccaagtatc atgataggca aaataataaa
    16141 taataacggg ctgaaagcct catttattgg attgcctgtt aaaccccatt tcaaaatttg
    16201 ttgcaaaaat accgagactg cggcactgat tactacattg ataaaaaaat atttggctat
    16261 atttttgaca ggagcgacag gtttcgagaa catttttttc gtatcttttt taccaaaaac
    16321 aagtgtaaat gctataaatc cagtgatagt taaagaaaaa atgctaataa tggatggcgc
    16381 aaatagcaac cctaacaaac cgagtacaac gacaatagtg cccaatatcc cttttttaag
    16441 ttctattttt tttcctaaaa tgttattcat gataataact cctactattc agtttttgaa
    16501 gatagagtca gtatacatga agagttaaaa taggcaatgt ttttttatgg atatttgtat
    16561 tgctaaaaat ttatcttata tattcatttt gattatacta cttttatttg taaaaatttt
    16621 ctagcaaaac caagtgcttg caactgatta agcgtacaat tttttagatg ttccgagcgt
    16681 acttcaatca tttcaaagga gttgctactt aaatcgatat tttttaaatc agtatcaaaa
    16741 aagctagcat tttctaaatc attagtagaa aacattattt tagtgaggga acagtctaca
    16801 aaatctgcgc ttgataagac agagtgctca aaggtacttt cttttatgtt tgaaccagat
    16861 aaatttacat agtccatttt acaattctcg aataacgtgt tcccaatata actttccatc
    16921 aatttagtac ctgttaattt acaattgaca aatttcacgc gatgcaacat gctttttgtc
    16981 aatatacagc ctgaaaaatc acagttttcg aatatacagt cggtaatctc cattctttga
    17041 aaatcattgg aagcaaaaaa tgcatacttg aaaatagatt tgtaaataat tggatggcag
    17101 attatttggt tttcgatgac ctctttttgt gaaaggatat tatacagttc aaaatcttcg
    17161 ttttcataga tatgaaaaaa ggatccttta ttcaagcttg cttcattaac tttaggaaaa
    17221 atcatattta attacctcca ttttcataaa gtcatattaa caaaagctaa accaacttac
    17281 attagcagaa aatgagatag gttttcgcct ttttcgataa cgatagtgtc gctatctctt
    17341 gaaacgttct tagaattgcc ctgagagaca caaattaggc gtaattctaa taaaaatctt
    17401 agaataaaaa tttttactga tttaatttag aatgttttta aaataaaaat cgttgccata
    17461 acagtgtatt gaaaggtttt aacaatccag taatgattta aaaaaatcta gatgtagcgt
    17521 atactttgtt tatagaatgt agtgggaaga aatatatttt tgggaggaaa tcgtgatgtc
    17581 taaaagtgaa gaaatagaaa acatggtact aaaattatat gatctcgttt taaatccaga
    17641 gatcaaagaa aaggaacgta tacttttgat agacgcaaag acaggcttag aaaaaggtca
    17701 gtattatcct aaagtcataa ataatttgga acgtagttta cgtccgttag caatcagggg
    17761 agaattgtct aaaccagtat caccgtttta tatggagata tcaacaatag gaaaatttga
    17821 aaaagaatta ggtcgaggaa tggcttcggc accgattact tttgggcatt tataaaaacg
    17881 agttattcat ctcaatgaat taaaaagtat agtcaatctc ataatgcagg ctaggttcat
    17941 ttatttgaac agagtctgta tttttttatt ctaaataaag aaacagatgt ttctttatat
    18001 tgtattttat cctttaatta acacaaaaac agctagtata aactaataat gttaattaaa
    18061 agtgtatttt atgaaactat cgttatttat taatgttgca tttttgtaag ttaaaaattg
    18121 gaaatataga ttgaaaaa
//
LOCUS       DACEPD010000059        17509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.061, whole genome shotgun sequence.
ACCESSION   DACEPD010000059 DACEPD010000000
VERSION     DACEPD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.061"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            7..849
                     /locus_tag="IW797_001878"
     CDS             7..849
                     /locus_tag="IW797_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ1224656.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            846..1796
                     /locus_tag="IW797_001879"
     CDS             846..1796
                     /locus_tag="IW797_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ1224657.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1811..2044
                     /locus_tag="IW797_001880"
     CDS             1811..2044
                     /locus_tag="IW797_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ1224658.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            2375..2959
                     /locus_tag="IW797_001881"
     CDS             2375..2959
                     /locus_tag="IW797_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1224659.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            3090..3758
                     /locus_tag="IW797_001882"
     CDS             3090..3758
                     /locus_tag="IW797_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ1224660.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            3760..5184
                     /locus_tag="IW797_001883"
     CDS             3760..5184
                     /locus_tag="IW797_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ1224661.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            5202..5771
                     /locus_tag="IW797_001884"
     CDS             5202..5771
                     /locus_tag="IW797_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ1224662.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            5881..7635
                     /locus_tag="IW797_001885"
     CDS             5881..7635
                     /locus_tag="IW797_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224663.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            7589..9361
                     /locus_tag="IW797_001886"
     CDS             7589..9361
                     /locus_tag="IW797_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224664.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            9884..10153
                     /gene="rpsN"
                     /locus_tag="IW797_001887"
     CDS             9884..10153
                     /gene="rpsN"
                     /locus_tag="IW797_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ1224665.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            10168..10347
                     /gene="rpmF"
                     /locus_tag="IW797_001888"
     CDS             10168..10347
                     /gene="rpmF"
                     /locus_tag="IW797_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1224666.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            10379..10756
                     /locus_tag="IW797_001889"
     CDS             10379..10756
                     /locus_tag="IW797_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224667.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            10777..10926
                     /gene="rpmG"
                     /locus_tag="IW797_001890"
     CDS             10777..10926
                     /gene="rpmG"
                     /locus_tag="IW797_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ1224668.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            10950..11933
                     /locus_tag="IW797_001891"
     CDS             10950..11933
                     /locus_tag="IW797_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1224669.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            11963..12085
                     /locus_tag="IW797_001892"
     CDS             11963..12085
                     /locus_tag="IW797_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ1224670.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            12103..13626
                     /locus_tag="IW797_001893"
     CDS             12103..13626
                     /locus_tag="IW797_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ1224671.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            13650..13889
                     /locus_tag="IW797_001894"
     CDS             13650..13889
                     /locus_tag="IW797_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224672.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(14082..14543)
                     /locus_tag="IW797_001895"
     CDS             complement(14082..14543)
                     /locus_tag="IW797_001895"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1224673.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(14738..14956)
                     /locus_tag="IW797_001896"
     CDS             complement(14738..14956)
                     /locus_tag="IW797_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1224674.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            15074..16690
                     /locus_tag="IW797_001897"
     CDS             15074..16690
                     /locus_tag="IW797_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ1224675.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(16931..>17509)
                     /locus_tag="IW797_001898"
     CDS             complement(16931..>17509)
                     /locus_tag="IW797_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1224676.1"
                     /translation="GRAFTNHRIQVMKTFKKGDRRSKHLKKYWKLLQKNAWELKGQHR
                     YWRPSFRDHLTEAEIVDRLLSYDDSLKRGYEVYQDFLSVIRRQDVPEFVALLKEDYKE
                     LPEHYQPVFTTFKKYQTEIKRALRVPYSNGPIECLNNHIKVLKRIAYGYRNFQNYRER
                     IFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         6058 a         2463 c         3329 g         5659 t
ORIGIN      
        1 aataatatgc ttcaaaattt tattaatggt ctgtatgact ttcacttttt acagaatgct
       61 ctaattacat ctgtagttat agggattgtt tctggaacag taggttgttt tattatttta
      121 agaggaatgt ctcttatggg tgatgctatt tctcatgctg ttttgccagg agtagcaatt
      181 tcttatattt taggtattaa tttttttatt ggtgcgattg tatttggtct attagcttct
      241 atcattatta cttttataaa aagcaatagt gtaataaaag gagatacagc aattggtatt
      301 actttcagtt cttttttagc attaggagtt atccttatag gagttgcaaa tagctcaacg
      361 gatttatttc atatcttatt tggaaatatt ttagctgtac aagatattga taagtggatt
      421 acaattggcg tttctgtttt agtgttggtt acagttgttc tattttttaa agaattatta
      481 atcacttctt ttgatccatt aatggcaaaa gcaatgggga tgaatgtcag tttttatcat
      541 tatctattaa tgattctatt aactttagta gcagtaacag ctatgcaaag tgtgggaact
      601 atattaattg ttgcaatgtt aattacacca gctgctacag catatttata tgcaaacagc
      661 ttaaaaacta tgatatttat ttcggcgaca gtcggagcgt gctcatcatt gctaggtttg
      721 ttcattggtt atagttttaa catagcagca ggatcaagta ttgttttaac agcggcattc
      781 ttatttgtaa ttagttttct aatctcacca aaacaaaaat ttttaagaaa gaaggagaga
      841 aagttatgat gagaaaatgg aaagcagtac tgggaagtct ggggattttg attgctcttt
      901 ttatattcgg ggcatgttca acaaatagta aagacaaaga tacagtggct tcgaacgaaa
      961 aattaaaggt agtagttact aattcgattt tagcagatat tactgaaaat atagcaaaag
     1021 ataaaattga tttacacagt atcgtaccta ttgggaaaga tccccacgaa tatgaacctt
     1081 tgcctgaaga tgttcaaaaa acttcaaaag cagatttgat tttttataac ggtgttaact
     1141 tggaaactgg aggaaatgct tggtttacaa aattagtaaa aaatgcgaac aaagaggaaa
     1201 acaaagacta ttttgcagca agtgatggca tagatgttat ttacttagag ggtcagagtg
     1261 agaaagggaa ggaagatccc catgcttggt taaatttaga aaacggtatt atttacgcta
     1321 aaaatattga aaaacagtta gcggaaaaag atcctgataa taaaaaattc tataaagaaa
     1381 atctagataa gtatattgaa aaattggatt ctctagacaa agaagctaaa tctaaatttg
     1441 cttcaattcc gaatgataaa aaaatgattg ttacaagtga aggatgcttt aaatatttct
     1501 cgaaagcgta taatgtgcct tctgcttaca tttgggaaat caacactgaa gaagaaggaa
     1561 caccagatca aataaaacac ttagttgaaa aattacgcac aacaaaagtt ccctccttat
     1621 tcgtagaaag cagtgtggac gatagaccaa tgaaaacagt atcaaaagat accaatattc
     1681 ctatctattc aacgattttt actgattcaa ttgcagaaaa aggacaagat ggtgatagtt
     1741 actatgcgat gatgaaatgg aacctggata aaattgctga aggcctttcg aaataatttg
     1801 gagggtaggt atggtacaaa ttaaaacgac tactatacta atttatttac tagcaattca
     1861 ggaattatca aaaagaaaaa agaaattaaa aaataatgat ttagcaaaag ttctaagtgt
     1921 tagtccagca tctgtaagtg agatgctggc taaacttaga tatgaggact atttagatat
     1981 aggatttcaa ttaactgata aagggaaaaa atttattgac gattacaaaa aaggttttaa
     2041 gtaaaagact ttatttttag ggaggataag aaaatggcta ttggagtgat tatgatagga
     2101 cttaccctaa tagctggata taattttttt aaatctttct cggattattt tagaaaaggt
     2161 gaatgattta gatgagtcta ttaattcttg taggattgct ttttgtatca gttattttgc
     2221 tctttctcat tagtttagct ctaaggaatg agttcagtaa tagacgttaa cgattgaaac
     2281 ttcaagtcct gtgtaagata atatagttaa ttcatcttat taggaggaag tacccataga
     2341 aaccagagat tatattacaa ctcaccaaaa attaatagaa agtggactaa gccattttga
     2401 aaaagaagga tttcagcaaa ctaagataag gcaactgtgc ttgaccgcag gcgtaactac
     2461 gggtgctttt tataaacatt ttacttctaa ggaagcgtta tttgaagaaa ttattcagcc
     2521 gcatattaat gcgcttatta atatgtataa tgaagagttt accaaatacc tcttaaactt
     2581 agaaaatata gatatagaga aaagttggag agagaacttc aatgaattag atccatttat
     2641 taattatatt tttgagaata aacgtatatt cgatttggtt ttattccaat cagctggaac
     2701 caaatatcaa gatattgttg aaaaaattac atcatttgtg acaaaaaaga cagtctgttg
     2761 tctagataag ttaaagacta tcggaatgat caatcaaaat ttagtcttta atgaagaaga
     2821 aatccacttc tacatttact cattctacgc tacattctat gatatattga agcatagcta
     2881 ttcaaaagaa aagactatgc tattaaccag aagattgtat caattttctg ttcctgggtg
     2941 gattgaatta ctgagttaat taaaaaaaag cttgcattga gaattgttct cagttatgct
     3001 ttttgataag atcgcactgt tatctgaata acggtgcatt tttgtcagct taagataaca
     3061 gtgcgatctt attttttgga ggtgaaagag tgtcaaaaat caatccgtta accttgatta
     3121 ctttaaatat tttttttccg gtcattattt ttttaggaaa gggaaatata tttcaagtgg
     3181 ggtgtttcat tattgctaca gttttagtat tgatgtttcg taaaataaag acatgtattt
     3241 atttcatctt gatttattca gttctgtttt tagtaatgaa aattattgat gctatcaaga
     3301 tagagttttt tactatattt tttgggacat ccttatatat tgtatttcga atgttgccgg
     3361 tcgtaatgat ctcatggttg ttggttagtg tctatactag caatgaatta ttatcctctt
     3421 tagaaaaaat acatttaccg aaaaaaataa tgttaagtat tacagtagtg atcagattct
     3481 ttccgactta tcgttctgaa ataagaatga ttaaagaatc tttaaaaatg agaaacattc
     3541 gcttgtcttt ttttcagcct ctgcaatatt tggaatttct gttagttccc gttcttatac
     3601 gtgcaactat gattgctgaa gaaatgaccg ctaatgcaat aacaaagggg atagaatctc
     3661 ctgtgaaaag aagttcttac tataaaacaa acatgagctt attagactat atcatcctgt
     3721 tttccgttac tatttttttc ttaagtattt tgatttgagg tgaaagaatt gtttgaattt
     3781 aacaatgttt ctttttcata taaggatgtt aaggaacaag cgcttgttga ccttaatttt
     3841 tcaataaaaa aaggcagtct tacattgatt actggttctt ctgggagtgg gaaatccaca
     3901 ctattaaagt taatgaatgg acttattcca agactttatg acggaaaatt ggttggggaa
     3961 attttatttg aaaataactc gctacaatcc ttaacacaag aaagtatatc aaaaaatatt
     4021 ggctacgtaa gtcaaaatcc tagaggacaa ttctttacaa caaatacaac gagtgagtta
     4081 gtattttcga tggaaaactt tggatttaca aaagagcata tggataaaag aatcaatgaa
     4141 ttagctgaat tattagatct ttcaaatata ttagataaaa acattttttc gctatcaagt
     4201 ggtgaacggc agaaaatttc tattggatgt agtttatcgc taaactctaa attaattata
     4261 ttggatgaac cgtcttcaaa tttggacttc aaagcgacta agaaacttgc tgacttactt
     4321 tgtgatttaa aaaataaagg atttactatt gttatagctg aacatcgttt ttactatttg
     4381 aaagaattaa ttgatcaagt tctatttgtt aaaaatagaa ctgtaaaaac tacaactatt
     4441 cagttgttaa aaactgaaaa caataacgca ttaaggaaat ttgacatttt taatagttcg
     4501 attgaagttc cctatatcga aaaggtaagt gcagatgtat taaaagttga agaaattact
     4561 tatgaaaata ttctaaaaga cataaatttt gatgtcaaag ttggagatgt tattggtttg
     4621 gtagggaaaa acggtgtggg gaaaacaacc ttactaagaa tactcatgaa gattattcgc
     4681 cctaccaaag gaaagataat cgaaaataaa aaaaatttgt catcgccgtt tttagtaatg
     4741 caagaaatgg attaccaatt tttcacagaa tcagtgagga gcgagttagc cctggggaat
     4801 gaaatagtta gtaaagatca gcaagaaaaa attttaaaaa aaatggaact ttataaaatg
     4861 aaagatcaat taccgtttga tttatccggt ggagaaaaac aaagattatt ggtttctata
     4921 gcttcattgt caaaaactaa tttatttcta tttgatgaac ctacaagtgg gttggattat
     4981 atcaatatgg aaaggattgc tgaccttatt aaagacttaa aagaaaaggg tgcagtagtt
     5041 atagccactc atgatccgga atttctttat aaaacatgca atagaattat ttatttaaaa
     5101 aatggtggaa taaaaaaaga tataaaactt agagtagaag attcaacttt gatagaacag
     5161 atttttaatg acataacgaa atagaattga ggagaatata tatgaaaaca aaagattata
     5221 tttttattgg tttagctaca gttatttccc tagtaattta ctttttttca ataatggtta
     5281 gtagtattgg tggggcgttt ggtcacagca tatccccagg gatttttgga ttattatctg
     5341 gaatcatatt tgtttatatt agctataact accctagaaa aggaattttt actatttaca
     5401 caattgtttt attgttattt tttacgttaa tgggcggggc gtacttacct tggtttatca
     5461 gttcgattag tatggcgatc ttagctgata ttattctatc tgtatttgga tatgatcgag
     5521 caattcctca agttgtgtct tgggttctaa tgcaactagg cagtgctgcc ggacagtgga
     5581 ttcccatatg gttctttaca gatcgttttc gtcaagactg gatagataga ggacaatctg
     5641 ctgcaacaat ggatgcaatg atccactacg cggtgggtat atggggaatt atttcagtgt
     5701 tagttgtagc aagtttatct atgattggtg tattgattgg aagaaaagtc ttaaaaaaat
     5761 acaaaaaata gaacgtagtt taccttctat ctgtttgtac atctctaatt tacgctatta
     5821 cggaaagcat taacagttgt aaacgaataa gagaagaaat tgaatgcggg ggaggaaaaa
     5881 atgaaagtat acaaaaaatt atttagttat gtccctaaaa tgaaatacta cggatggtta
     5941 gccactttat tttctgattt ttctgtcgta ttaacagtct atggatatta cagtatttac
     6001 aaatttcttc agtctctaat tattgtagga gatgaatatt tagctaagag ttatgcggtt
     6061 caaactgttg cttggttaac tttaggagct ttattttata ttttttctgg cttattttct
     6121 catatccttg ggtttcgctt agagactaat ttgagaaaaa agggaattag cggtttaaca
     6181 gaagcaagtt ttcgtttttt tgatttaaat tcttcgggat atattcgaaa gacaattgat
     6241 gacaacgcgg ctaaaacaca tatggcaatt gctcacttga ttccagatag tgctcaagcc
     6301 atagtaacac ctgttttatc agttgcctta ggctttttca ttaactttaa aattggattg
     6361 gttctaatcg ccatgttagt aattggtgta ttcttgttaa agaaaatgac agagaataca
     6421 aattttattc aaaaatatca agaatcgtta gatgtgttaa gctctgaaac ggtagaatat
     6481 atccgcggaa ttcaagttat aaagatattt ggtgctaaag taacatcatt taaagccttg
     6541 aataaagcaa ttaacgatta tgctaagttt gcatataagt attctttgag tgggaaaaca
     6601 ccttttgttc tttttcaatg gctatttttt ggcatagttt gcattttgat agttcctata
     6661 acctttttta taacttctct taccactccc aagatcttag ctgttgactt gttgatgttg
     6721 ttttttttaa gtggaattat ttttgtttct ttcatgagaa taatgtatgt aagtatgcac
     6781 ttgtttgaag caagatatgc agtcgaaaca ctagactccc tttatttaga aatgaagcaa
     6841 gaccaactaa aatatgggaa cgagacacag attaaaaatt gtgacattgc atttgaaaat
     6901 gtcagctttt catacaaaga agagtgtgtt ttagaaaatt tcaacttagt tttagaacaa
     6961 aaaagaattt atgcattagt aggaaaatca ggaagcggaa agagtacgat agctaaacta
     7021 atttcaggtt tctacaatgt ggataaggga cagattaaaa ttggtggtaa gcctttaaat
     7081 cactattcaa aagacacaat tattaacaca attgcttttg tctttcaaga tacgaaacta
     7141 ttcaatcaga gtatttacga taacgtttct atagcaaatc ctcaagcgcc agcaagtaaa
     7201 gtatttgacg cgctaagatt agccggttgc gaatcgattt tagaaaaatt tcccgaaaga
     7261 gagcatacct taattggtag taaaggagtt tatctgtcag gaggagaaaa acaacgtata
     7321 gctattgcca gagcaatttt aaaagatgca aagattgtta ttatggatga agccagtgca
     7381 tctattgatc cagataatga gtatgaacta caaaaggctt ttcaatattt gatgaaagaa
     7441 aaaacagtta ttatgatagc tcatcgatta tccagcatta aatctgtaga tgaaattctt
     7501 gtcttgtcag aaggaaaaat agtagaacgt ggaaatcata atgaattaat ggctaaacat
     7561 cgagtgtata aacaattggt cactaattat gagaatgcaa atgattggag gcttgacaat
     7621 gaaaaatttt tataaaaaaa aatttgcttt aactgatcag ggcgcagaag ctttaactaa
     7681 agcttctatt tctagttttt ttgtatactg cattaatatg gtaccagcat tcattataat
     7741 gatgctgata gacgaattag ttttagaaaa tgcaaagccc cgttggcttt attttgcagt
     7801 atcgtttgtt actttgcttt tcatgtattg gttgttagat agggagtatg aaaacttata
     7861 taatagtact tataaagaaa gtgcgcattt aagagtgcaa attgcagacg atttgtcaaa
     7921 tttaccatta tcctattttt caaaacataa tttatcagat ttatctcaaa ctatcatgtc
     7981 tgacgttgaa ggtattgagc atgcgatgag tcatgcaata cctaaagccg gcggtatggc
     8041 tctgtttttc ccttttattt cagtgatgct tttggttggt aatgtcaaaa tgggattagc
     8101 tgttattttg ccaacgttat ttagttttgt cttaatcttg ttatcaaaga aatcccaaac
     8161 gaaagccaat actaaatatt acgatacttt gagagaaaac tcggaagaat ttcaagaaac
     8221 tattgaattg cagcaagaga ttaatagctt taatctatct aaaaaagttc aagacagact
     8281 tttcaaaaaa atggaagaga gtgaaaggat tcatttaaag gtagaattaa gtactttttc
     8341 agtcatggcc ttatcctcta ttttctcata tgttagttta gcagtagtca ttctagtagg
     8401 tgttcactta ctgttaacgg gtgaagtgac tatactctac gtcgttggtt acttactagc
     8461 cgcaataaaa ataaaggatt ccttcgattc tatgaaagaa gcagtgcttg aaatatttta
     8521 tttagctcct aaaatacagc gaattagagc tatgaaggaa accagtatcc aagagggatc
     8581 agatagtcct ctgaaatctt ttgacattga gttgcgagat gtctctttct catatgataa
     8641 caatacccca attttagatc atatctcttt tacggctaaa caaggagaag tgactgcgtt
     8701 agtaggtgct agtggttccg ggaaaactag tatcttgaaa ttagtgtcta gactgtatga
     8761 ttatgatgaa ggctgtattt taattgatgg gtatgatatt aaacgtgtat ctccagcttc
     8821 tttgtttagc aaaatcgcta ttgtttttca agaggtaact ttgtttaata caagtatttt
     8881 ggaaaatata cgtattggaa atagtcaagc gtcagatgag gaagtgaaaa aagcagctcg
     8941 tttagctaac tgcgaagact ttattgaaaa actacctgac ggttatcata cattggtcgg
     9001 agaaaacgga agttcacttt ctggaggaga gcgacaacgc ctatccatag cacgggcttt
     9061 tttgaaaaat gcgccaattt taattttaga tgaaattact gccagtttag atgcggaaaa
     9121 tgaaaaacgt attcaggaaa gtttaaatcg tttaatacaa gataagacag tactaattat
     9181 atcgcatcgc ttgaagtcta ttgaaaaggt aaataaaatt gtagtgatgg atcaaggaaa
     9241 actagttgat caaggtactc ataatgagtt gtatcgaagg tcagaaatat acaaaaatct
     9301 cataaagaaa actaaactat ctgagaaatt cgtctatgaa aaagaagctc aatcacgatg
     9361 atgaaatagg ctttaaatta acctacaatt tttgggatat agttataatt ctttttcaaa
     9421 aagaataatt ttattaaaca gcatctaatt ttgaatacac gaaagaatca gtatgattga
     9481 attagttcac ttaatgtcta ttcttataat ataaggaata atcaaagttt taaaaaattg
     9541 gcgataaacc agttaaacat aaaagattga ataaaaagtt atagtttctt tcatattgcc
     9601 gttttaagta gaggaaaagc tacttaaaac ggtatttgtg tttgattgga ctaataaaag
     9661 tgaattctta tttttcagag cctaaactgc atattctttt aaatgatttg attatctcta
     9721 tgtaccgata aattcgtttt caaatagtta tctattcata tgagttttat ttcatgttat
     9781 ttattgttga caagagcaac tcttgttggt aaagtatcag tgtcaaaacg taacgattac
     9841 gatttgaggt gtctaaatca aaataaaagg agtataaagg tatatggcaa aaaagtcgaa
     9901 aattgctaaa gcaaagaaac aaatggcgat gattgaaaag tatgcagata aacgtcagga
     9961 attaaaagcc gcaggagatc gcaccgctct cgctaaattg ccaagagatt caaatccgaa
    10021 tcgtctgagg cttcgtgatc agacggatgg tcgtccaaga gggtatatgc gaaagttcgg
    10081 tatgtcccgt attaagttta gagaacttgc ccatcaaggg ttgattcctg gagtaaaaaa
    10141 agcaagttgg taaaaggagg aaatatcatg gcagtaccag caagaaaaac atctaaggcg
    10201 aagaaacggt taagaagaac tcatcaaact gtagtaaaac cagaaatttc atttgacgaa
    10261 gcgaatggtg attatagacg tagccatcat gtatctttaa aagggtacta taatggaaag
    10321 aaagtaatta aaggagcttc aaaatagttt aatatataac ttttaggagg gaatcaaaat
    10381 ggaaattatt tacccacctt tagttgaaga aggattaaag tattatcttg aaacgaccca
    10441 gcaatcgcta gataaatgca ctttttatcg ttcgatggta gagagaggaa ttattactga
    10501 aacgggttta ccgacccaac aggcaattga aaatggatta gtcaaagatt actatgagga
    10561 ccaaggctta tcatttgatg attttttaag aatctatcca atttttgaag agtatgacga
    10621 ggagttattt caatgtatcg atggttattg ggagatacct gttgatatga aagaaaattt
    10681 agtttcacaa ttggaatctg gcgagttaac ttttgaagat gcacaacaaa ttcaagcata
    10741 tcttgaagat cgataagtat tgaaggagga agatttatgc gtcaaaatat tattttggaa
    10801 tgtgtcgaaa ctggagaacg tctgtatctc accagcaaaa ataaacggaa taaccctgaa
    10861 cggttagaat taaaaaagta ttcgccaaaa ttgcgcagac gggcaatttt taaagaagtg
    10921 aaatagctaa gtggtaaaaa ggtgacaaaa tgggcatacc agttacaatc gtttctgggt
    10981 ttttaggggc tggaaaaaca acgctaatca atcaagcctt gcaagtctca ccctacccca
    11041 gggaagaaat tattataatt gaaaatgagt ttggtgaagt tgggattgat cacaaattat
    11101 tagttcatag cgaggaacgg gtgttacaac taaataatgg atgtatgtgt tgcagtcttc
    11161 gcagtgattt attagcaata ttgatttctt tactggaaac aatagaagaa catcaacaac
    11221 caatttcaca aatcatcatt gaaactacag gtatagcaga tccccaacca atcattcaaa
    11281 cacttttaac agcgcctcaa ctaagaggaa gagtatatat cgatagttta ttgactgtta
    11341 ttgatagtga cagttttgaa cttattgaaa gagaaccaca agcccttaag caacttgtca
    11401 tggcagatcg catttttgtg tctagaaaac aaaacttgaa tttatctgca ctaaaaaaat
    11461 tacagagaga aatcaaaagt attaaccctt tatccgatat tcgaatgttc acacatagtg
    11521 aaacgattca aaaaaaggat ttttataatt tgaaaaaatt taatcatccc ttagtttttg
    11581 aaagtgattc atcagaaaat cactataatg gcaaatacta tcatcaacac catcaagtac
    11641 atgagtttaa atcaattctc ttaacttcag atcaagacat aaaaaaagat tttttactcc
    11701 aatggattga ctggctgatt ttaaccaacc aagggagcct gtatcgtttt aaaggattga
    11761 ttgctttaca agagcaggat tttatagttg ccatgcaaga ggtaaacgaa caggttaatt
    11821 ttcaatacac cactcgacaa aaagaccaaa ccactattat tctaatcgga aaaatgttgg
    11881 atgaagaaag gatcaaacag agttttgata aattactggc ggaaagcaaa taatgataag
    11941 tagtgattgg gggtaatttc taatggagaa aacggatata tctagtgcat atcgacgact
    12001 taaaagcccg aatattaaaa ctagaaaaag ggcattaaaa attatcaaag aacataaacg
    12061 aaataaaatg aagaaattag cataattaag gaaggaaaaa gaatgaagaa aatcagtgta
    12121 ggacttattg ggatagcagc tctaggatta ttgggagctt gctccagcac taatgatgca
    12181 aaagtatcga atgataaaga tggaaaatta gaaattgtaa caacattcta tccaatgtat
    12241 gatttcacca aaaacatcgt cggcgatgaa gcgaacgtgg atttaatggt tccagcggga
    12301 tcagaacctc atgactatga gccctcagct aaggatatgg caaaagctca tgatgctgat
    12361 gtttttgtat atcataacga aaacatggaa tcatgggtac caaaagctaa agaaagttgg
    12421 aagaaagctg ggcctaatgt agtagaagga acaaaagaca tgattttatt gccaggtagt
    12481 gaagaagaag atcatgatca cggagaagaa gatcaccatc atgaactaga tcctcatacg
    12541 tgggtttcac ccaaaatggc aattaaggaa gtttcaaata tcaaggatca gttagttaaa
    12601 ttgtatccga aaaaagcaaa agtatttgag acaaatgctg aaaagtattt gactaaactg
    12661 aaacggttgg atgctgacta taccactagt cttaaagaag cgaaacaaaa aagttttgtg
    12721 acgcaacacg cagcctttgg atatttagct ttggattatg gattgaaaca agttcctatt
    12781 gctggactaa gtcctgaaga agagccatct tctggtcgat tagctgaatt aaaagagtat
    12841 gttaaaaaaa ataagatcaa ttatatttac tttgaaaaga atgctaatga taaaattgca
    12901 aaaactttgg ccaacgaagc tgggataaaa ttggaagtac tgaatcctct tgaaagtctt
    12961 acaaaagaac aaatggataa tggtgaagat tatgtttcag taatggaaga caatctaaaa
    13021 gcgttagaaa aaacaactat ggttgctggt gaagaggtag ttccagaaaa agaagcgaag
    13081 gatgaaaaga cagttgctaa tgggtacttt aaagatgccg atgtgaagga tcgcgaattg
    13141 agtgattata caggcgaatg gcaatctgtt tatccgttgt taaaagatgg aattttggat
    13201 gaagttttcg attacaaagc aaaaatcaat aaagacatga cagctgccga gtacaaagat
    13261 tattatacta ctggctataa gacagatatt gatactatca acattaaaga caacaccatt
    13321 gattttgtag tcaacggtga acatcatcaa tacacataca aatatgttgg atacaaaatt
    13381 ttgaattatg aaaaaggaaa ccgtggtgtt cgttttaatt ttgaaactga tgatgcaggt
    13441 gctggtcgtt ttaaatatat tcaatttagt gatcatggaa ttgcaccaag taaggcagag
    13501 catttccata ttttctttgg tggagaaagt caagaaaaac tctataatga attgcataat
    13561 tggcctactt tctatccagc ttcattgtct aaacatgaaa ttgctcaaga aatgatggct
    13621 cattaaaatt caatttttgg aggaaataca tggtaagaaa agtattattg tctatggatt
    13681 ccaagaatat tgatgcttat cggcgttatc gatttgcaaa gcaaatgaga ttaaatggga
    13741 gtacattagc tgatattgct gcaactttac atgttagtat tgacacagtt catagagtat
    13801 taaaacggga tcctgattgt tgttttatca aggtcaatac tggaagaagt agacgtttag
    13861 caaaacggca taatagcttg gccaattaat tgataatacc agagtagatc atttttccct
    13921 tatttggatt tgatctgctt tttattatgt cttgaattgt caaatcaagc gcaacacttt
    13981 atcaaagaag acttcataag gtgttctcca atctaaacac tttctaggtc tgttattcag
    14041 tttttcaatc atctgattta tcgttgagtt atctaccgta gctatctctt tcgattttgg
    14101 caagtattct cgaatgagtc tattcgtatt ttaatttgtt cattgttgcc aagggcatga
    14161 ggatctggaa aataaaaggg catattattc aatgcttctg taactgcgaa atgattggag
    14221 aattcttttc ctctatctgg agtgatcgtt ttgaccgcct catttggaat actgcttaaa
    14281 agttcaatca ttttctcgct cacataagcg gctttctttc tggggacctt ctttcctgct
    14341 aaaaggaagc atgattttct gtctgtcaaa gtcactaaac aagtaccacc agttttgcca
    14401 gctacaatat cggctttcca atgaccaaaa tcacttctat catctgcgga ttttggacgg
    14461 ccatggatcg aatgagtaag gcaaatcttt ccacgagact cgacatgatt ttttgtatgt
    14521 cttgtttttc ctcgatggtg caatgaacgg atcaggcctt tattgccgtg tgagaggggt
    14581 tctgtatcga aaagatcatt gcaaattgca cgttagatcg tgataaaact aattgaaaag
    14641 tccatatgtt catgcttcaa acgattcgag atttcctctg gcgaccactg aaaatcgaca
    14701 aataaatgac gaacaattga ccaaactcac ggatcattta gaatgtgata acgtccgcat
    14761 ttttttcttc gataagcata atttttttcc gctttggaag gtgaatagtt tttttcttgt
    14821 ttcttctcag ttcatgtaaa atagtaaacg ggctatgctg aatgtcgttg ccaattatac
    14881 taatagaaaa tccttcttcg tgaagcagaa agattctttc tcgttcgagt atagtaagat
    14941 gacgatactg gctcataatt attttccttc taggagtgtt ctcgacaaac ttatactagt
    15001 tggatatcga ttatgactca tttttatgtg ttgcacttaa agtgtaaatt caaggtatag
    15061 aaagaggttc cctatgaaaa aaatacctat tacaatctta actggctatc ttggttcagg
    15121 aaaaacgata cttctaaatc atattctgta cggtgatcac ggaaaggaaa ttgctgtcat
    15181 tgtaaatgac ataggggaag taaatgttga tggggtatta gtcgacaaga aaggttttaa
    15241 aagaactgga gaaaaacttg tttcgatgcc gaatggatgc atttgttgta cattacgcga
    15301 agatttgatt gttgaactag atcaattagt gcaattttct gacttgaatc aaattgttat
    15361 tgaagcttca ggaatcagca aaccaattcc aattattcaa gcaattatat tagctaaaaa
    15421 ccctttagac attgatttga cccaacgatt agagattggt gccattatta tagtagtgga
    15481 tgtaaaacgt ttttgggata atttcgaaac gggtgaaaag attcaggaaa gaaaagctga
    15541 aggtaatgta gttgaagaag aacaggatat tcgtggcttg ttaattgatc aattcgagtg
    15601 ttgtaacata ttacttttaa ataatagtga cttagtgtca accaagacga tttacaaaat
    15661 taacggtcta attgctaagt tacagtccaa tgccaaaata attaggacaa ttaaaggtga
    15721 agtagactta tcgaaaattc taaatacaaa tctttttaat atagatgaga cttcctattc
    15781 agtgggctgg ttcaaagagt tagagcttgg ctatcaaaat catattcctg aaacagaaga
    15841 atatggtatc tcaagtttta tctatcgaaa tcggttactc cttgacgcac aaaaatttgc
    15901 ccatcttacg gataaaaatt ttcctggcaa tattacaaga gcaaaagggt ttctttggtt
    15961 tgcccaacat agtgattttt cgacgatctt tgaaacatcg ggtagctata aaagtattga
    16021 atcattaaat tattgggtag ctagtttacc taaaattgag tgtcaaatga ttatacaaaa
    16081 aaatcccgaa ttgaaggaag cctggcatcc gttgtttggt gaacgcatta ataaactagt
    16141 ttttattggt atgaagatgg atcaacaatg tattaaggaa caacttgagg gttgtttaac
    16201 tgattctctt aatgcagata atttccgaaa aatttcacca tttccatgga aaaagtctaa
    16261 tatgtttagc cagatatcgt taatgaaatt tttaagaaaa gctggtgtca agataggcga
    16321 tgtcataatt attggcattt tgattattac ctcttttatt cccttatttg tattctctat
    16381 agaaaaggag aatgaaacaa ctgaagatgc tatcaaatat gccatcgttc gaattgatgg
    16441 caaggaaatg gatcgcttcg atttggataa gatcaatcat aaactcgtga attatcatcc
    16501 tgcgaaggga caatataata ttgttgagat taaagatgga agaattcgag ttaaagaaga
    16561 taatagtcct gatcaaatcg cggttagaac tggatggatc tcaaaacccg gtcaaacgag
    16621 tatttgttta ccccataagt tagttattag tattgagaaa aaagaaagca cagattacta
    16681 catttactaa tctggaaata gatgaggagg tttttaataa gaaaaagata ttattattaa
    16741 taatagcagg ggctttatta agtgtgttaa gcgcttgtgg acaagaaaaa gaagcggtga
    16801 caacaggcac aagtatcttt aacgggacct gcaatggttt agttaacagt cggttagatt
    16861 acttattttg gctcttcgtc aatgagtgtt ggtgaagaaa ttcaatagaa taatcgagat
    16921 actcacacgg ctagacagct atcttgtcta gccgtgttgt tgttctggct ttagtcagtt
    16981 gggtagtatt cttagttttc ttaaaatatt ttcctcgata taaaaagatt cgttctcggt
    17041 agttttgaaa gtttctatag ccataggcaa ttcgttttag tactttgata tggttattca
    17101 aacattctat agggccgttg gaatagggaa cgcgcaacgc tcgtttgatt tctgtttggt
    17161 atttcttgaa ggtcgtgaac actggttggt agtgctctgg caactccttg tagtcttctt
    17221 ttaggagcgc gacgaattca ggaacatctt gtcttctgat aacggaaaga aaatcctggt
    17281 agacttcgta gcctcgcttt aaggaatcat cataagataa tagccgatcg actatctctg
    17341 cttctgtcag atgatctcga aatgacggac gccagtagcg atgctgacct ttcaattccc
    17401 acgcattctt ttgaagtagt ttccagtatt tctttaagtg cttcgaccga cgatcccctt
    17461 ttttaaaggt cttcattact tgaattcggt ggttggtaaa agcccgacc
//
LOCUS       DACEPD010000060        17090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.062, whole genome shotgun sequence.
ACCESSION   DACEPD010000060 DACEPD010000000
VERSION     DACEPD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.062"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(70..615)
                     /locus_tag="IW797_001899"
     CDS             complement(70..615)
                     /locus_tag="IW797_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1224677.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(627..1319)
                     /locus_tag="IW797_001900"
     CDS             complement(627..1319)
                     /locus_tag="IW797_001900"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ1224678.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1338..1622)
                     /locus_tag="IW797_001901"
     CDS             complement(1338..1622)
                     /locus_tag="IW797_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224679.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1635..2198)
                     /locus_tag="IW797_001902"
     CDS             complement(1635..2198)
                     /locus_tag="IW797_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ1224680.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2426..3088
                     /locus_tag="IW797_001903"
     CDS             2426..3088
                     /locus_tag="IW797_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ1224681.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3212..4408
                     /locus_tag="IW797_001904"
     CDS             3212..4408
                     /locus_tag="IW797_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ1224682.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4509..5885)
                     /locus_tag="IW797_001905"
     CDS             complement(4509..5885)
                     /locus_tag="IW797_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1224683.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5922..6788)
                     /locus_tag="IW797_001906"
     CDS             complement(5922..6788)
                     /locus_tag="IW797_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ1224684.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6877..7902)
                     /locus_tag="IW797_001907"
     CDS             complement(6877..7902)
                     /locus_tag="IW797_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1224685.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7920..8594)
                     /locus_tag="IW797_001908"
     CDS             complement(7920..8594)
                     /locus_tag="IW797_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1224686.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8778..9671)
                     /locus_tag="IW797_001909"
     CDS             complement(8778..9671)
                     /locus_tag="IW797_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ1224687.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9745..11442)
                     /locus_tag="IW797_001910"
     CDS             complement(9745..11442)
                     /locus_tag="IW797_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ1224688.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11435..12748)
                     /locus_tag="IW797_001911"
     CDS             complement(11435..12748)
                     /locus_tag="IW797_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1224689.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12748..16266)
                     /locus_tag="IW797_001912"
     CDS             complement(12748..16266)
                     /locus_tag="IW797_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ1224690.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16559..17017)
                     /locus_tag="IW797_001913"
     CDS             complement(16559..17017)
                     /locus_tag="IW797_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1224691.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4804 a         3846 c         2959 g         5481 t
ORIGIN      
        1 aaagtcgttt gttttatgct cttcttttat tattttgtgc tttttatttt atcaaatcac
       61 tttgtttttt tataaaattt ctgcgcctag tgtggactca aaatgtctca atgcccattc
      121 atgtccaacg ggatcgaaac ttgccacggc atctttgaaa atatggagtt tgtatcctag
      181 attatacgca tcgactgctg tatgcaacac acagatatct gtacatacgc cggtcaaata
      241 aatatctgtg atctggcgtt ctcgcaaacg gatgtctaga tctgttccac taaaagcgga
      301 ataatgccgt ttgtctatcc aataaacatt ttcctcttgc tcatagtctt ggtataaact
      361 ttttaaagag ccgtacaatt cccgtccacc cgttcctatc acattatgcg gaggaaataa
      421 gcggttctcc ggatgaaagg aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa
      481 atccttttga tcaataaact tctttgtatg agcagtcagc tttttttcaa ttacttgtcc
      541 tgctgtccca gtagtcagct ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga
      601 gatcaatgcg ttcatcttag tcctccttat acgagatgtt tgacaaattc gatcaccatt
      661 tctgctgatc gtttgcctgc gtcttcgata aattcgtcaa agctttgtgt cgccgagtga
      721 tctgctgtgt cgctcattgc acgtacgatc aaaaatggga tattgaactg acgagctgtt
      781 tgtccaacag cggctccttc catttcgcaa gctaatgctt cagggaaatt cgttaagatt
      841 tctttgattt tatctggcga gtcgacaaat gtatcacctg taacgatcaa tccttctttt
      901 gcgttcaaat tcgttgcttt tgctgcttta accatttcac tgcgcaaata tgtgcttgct
      961 tcataatata gaggcattcc tggtaattgt cctgggttgt agccaaatcc tgttgcatct
     1021 gcatcaaaat aagctacttt gtctgatata acgatgtcac ctacttgtaa gccttcaccg
     1081 attccgccag ctgaacctgt attgatcacc atgttcacgc cgtattgttg gatcaacaga
     1141 cttgtggtaa tagatgctaa gacttttcct ataccggaac gtacgacgat cacttcgtgg
     1201 ttacctaatg aacctgaaat aaataaagcg cctgcgcgtt cccatgataa tggttcgctt
     1261 aggttctctc tcaaaatttt tacttcttct tccattgcgc cgataatacc gattttcatc
     1321 tttttaattc cttctttcta tataaatgcg attgctaaaa aaaccacaca taacattagg
     1381 ataacaataa taagagattt catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg
     1441 ctgatccttg ttttcgtgat tggtttattc tttttgcttt ctttacgata aaagctggca
     1501 atcttttttt cttcttgctg ataagctgtt tctgcttttc gctgtttttt taatgactcg
     1561 cttgcttgtg cttgttgtcg cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc
     1621 ataagccgat ccctctactt tcctttggtc aatagttccc agtattgaat cgcataaatc
     1681 gtctttgcat cacaaatcaa ctgatctttc atcgcttgtt gcgcctcttc aagagttaga
     1741 tgatacaact ccagtacttc gtcttcatct tgtgctaaag gattctcgac cttctctacc
     1801 ccttgtgcat gataaatatg aaggacttca tttgcaaaac ctggtgaaag atacatcgaa
     1861 gtaagatggc tcagcgactt cgcacgatag ccagtttctt cttctaattc tctagctgct
     1921 gtcatttcgg gattttgtcc ttctcctgga tcgatttttc cagctggaat ctccaaaatc
     1981 actttttcta aaggttttcg aaattgtttg acaagaagca gacgatcttg ttcgtcaaaa
     2041 gcaatgatcc cgacaccgcc aggatgaaat accagttctc tcttcgaagt tcctccatct
     2101 ggcaaacgaa cttcgtctac agcaacatca ataatttttc cgctatagat ttctttgcga
     2161 ctgattgttt tttcttcaaa ttgctcattg ttcagcatat cttctcctgc tttctttgta
     2221 atcataactg cttttttttc atttttcaat ttttctcaaa aactccttat tagtttacca
     2281 tagataaacc ttagattttc tctaaatata gaaaatcctt ctttagtccg atactcttta
     2341 tagtagtccg tatgctaaaa taagtatgta atcgaataac gaaaagaatt caatactcaa
     2401 cgataaatga catggagggt ttattatgat aaaacgaaat tggccgtggc ttctagcatt
     2461 ggtcttgcta tttctctttg tccgaaacaa tagtttttct tttgttctag gacttttatt
     2521 gataggtgta gttttagttt tgtggggact ctttggttcg cgcaggaaaa agggaaaaca
     2581 agaagaaatt cctgcactga ctgacgaact tgaaactcac tatgaaaaaa gcggtatgac
     2641 agcgaatgaa atttcatttt ttcgacaaac aatgaatcaa acaaaaaatg agatcaatca
     2701 attgcaaatg aatatgcagc agacagccaa acttaaagca atcgatctcc gccatgatcc
     2761 agtcaaagca gcgaaagctt tattcaaaga attggtgaaa gaaccaaacc gactgcatga
     2821 agcaagtcag tttctttata cgcatctgcc gaatctagtt gatttgacaa ataagtataa
     2881 tgagatcaat gaccatgaga tcaaaaacaa acagacctat gaaaaactag aagaaagcgc
     2941 gcagatcatc gaccagctgt cagctttaat cgcacaagat tatcaaaaat tcgttgctga
     3001 ggatctagat gatatcgacg tagagatatc tgttgcgaaa cagagcttaa agcgtgataa
     3061 taaatatgaa ccagaacaaa aggattaact cttttgttca taaaagaaga ctcggaacat
     3121 tcagatgatg tctggctgtt tcgagtactt tttctaaagt agagacattc atgtaagaat
     3181 cgagaacaga ttcaacaagg aggaattttt tatggatcat aaaccgactg aaacaaaaga
     3241 tgttacacca gttaacgata cacttgaaga tttattgaac aacccgtttt caactccaac
     3301 agatacgctg accacttcac aacaagcgga gattgatcag ctccaagagc aacagacagc
     3361 tgctcgttta attgacaaac taccggcgga acgccaagaa caagctaaac agctagcagc
     3421 aaaaatcgat gctaatgatg cacagtctgt catcagctac ggatcggctg cccaagcaaa
     3481 attaagtgaa ttctcacaat cgatgttgaa tcatgtgcaa gcacaagata ttggtcctgt
     3541 aggagattcc ttaactgaac tgatgtatcg tctccaagaa gccaatcctg acgaactgcg
     3601 tgccggagaa gggaacatct tccagcgtgt cttcgggaag gtcaaacaat cgatctatga
     3661 agtgacagca aaatatcaaa agattggtgc acaaattgac aagatttcag tgaaattaga
     3721 taaagaaaaa gacggtctgc taaaagataa tctgatgtta gaacagcttt accagaaaaa
     3781 caaagactac tttgatgcac tgaatattta tatcgctgct ggagaattga aaatggaaga
     3841 acttcaaaca acgatcattc cagaagcgat gaaaagagca gaagaaactg gcgatcagat
     3901 ggatgttcag attgccaatg attacacgca attcctagat cgtctggata aacgtacgca
     3961 tgatcttcgc ttagctagac agatcacgat ccagcaagca ccacaaatcc gtttgatcca
     4021 aaacacaaat caggccttgg ctgaaaagat ccaagcatct atcgctacag ccattccgct
     4081 gtggaaaaac caagtcgtga tcgcattgac cttgcttcgt caaaaagatg cagtaactgc
     4141 tcaacgccaa gtctctgaaa caacaaacga cttattgaag aaaaattcag aaatgctgaa
     4201 aatctcagcc atcgaaacag cgaaagaaaa tgaacgtgga atcgtggata tcgagaccct
     4261 tcaaaccact caaaatgatt tgatcgaaac aatccaagag acattgcgta tccaaaaaga
     4321 aggaaaagaa aaacgtcgtc atgcagaagt agagcttgga cacatggaag aagatctgaa
     4381 acaaaaactg ttggatttaa cccaatagat cacctttcgc ttgttactaa aacaaacgcc
     4441 caacaacagt ttattacaac tgttgatggg cgtttttctt atctttcttc taacttctcc
     4501 attggagtct atttctcatt catcttgctg tgtccatagc catagccaaa ataaatgatc
     4561 agtccaataa ccattacgat accgaacacg atccatgtca cagtttgcag acgaagcatc
     4621 aatacaatac aagtcaagat ggagacgatt ggcaatagcg gaacgaatgg cactttgaat
     4681 tctccagctt ttggtttgcc aaacattttt ctcaacttca gtaagcctag actaacaagc
     4741 atcaaatagg cgagcgccat gatattcgtt agttccgcta atacattcaa tgggaaaaat
     4801 ccggcaaaga atacagctgc cacaccagcg gcataagtcg cttttttcgg tgtacgatgc
     4861 ttttgatcga tttctgtcat aaacttagga agtaagccat ccttgctgac agcaaacagt
     4921 aaacgagcaa gtgaatacat catcgagatc gttaccgtca gtagcgttaa aattgcccct
     4981 acagcgatca ccgcgcccac tgttccatgt cctacatacc tcattgcaaa agccactggg
     5041 tcattgactc ctagttcagt atacggaacg attcctgtca agatcagcgt cacgatgatg
     5101 taaaggacag tagcaatcag tatcgaacca atgattcctc ttggcacatc tttttgcggg
     5161 tttttgactt cctcggctgc cattgcaacg gcatcaaatc ccaagaaagc aaaaaacaca
     5221 agagctgcac catctagcac accggaaaaa ccaaaaggca tgaatggctg ccagttatca
     5281 ggtttaacga aaaatattcc tacaatgata aacaacgcga tgataccaaa tttgacatat
     5341 accatggcat tattcaaacg taatgctttc ttggcttcaa gcgacacgat gtaagtgacg
     5401 aatatcacta cgagcaccgc aatcaagtca atgtatgtac cattttccgg attgtacccg
     5461 ccagttaatg cttgtggaag ttcggcccct aaacttttca aaaaaccttg catatagcca
     5521 gaccatccgg aagcaacaga ggaaaccgct agtaaaaatt ccccgatcaa caaccaccct
     5581 gtcagccagg cagcaaattc accaaataca acatacagat aggcatatgg cccaccaatc
     5641 actggcactc gcgaagcaaa ttctgcgaaa cttagtccag acaaaatgac aacgattgca
     5701 gctaagacaa atgaaagtga cagtgctgga ccagcatttt gattggccgc aacccctgtc
     5761 accacaaata ttcccgtacc gatgatcgca ccaattccaa gcatgatcaa atcagacgtt
     5821 tttagttctt ttttcatctc actcgtatga ttgagattga tctctttctt acgaaacaaa
     5881 tccattgaag ttcctcccca ttttaggttg gaaaacagcg attaaactgt tttcatatac
     5941 cctaaaagga taccacctaa aatgactaaa aggcaaccaa caataacata aatgagctct
     6001 tttttcgttt ttctttcgcc aaggatgaaa atcccaccta aagtagagat aatgatcccc
     6061 atttgtgaaa gtgagaagcc aacagctaaa ccaagtgatt tgactgttag aagcatgcag
     6121 atgtttccta agccccataa taatccacag atcatgtttt tccacacaaa ctgatcgact
     6181 ttaactttac ggaaagcaaa gaagcttgcg cctaagatca ttccgatact ttgaggtaag
     6241 ataattgaca tcgcatccaa attcgcccag ttgacgatga tggtatatac accataacca
     6301 atcgtagaga agatcaatgc tctaaaaccc ttaccaaaat caagcatttt attatctgta
     6361 tctatttttc cttcgtcatc ttgtcgggca gtcaaatacg caccgcttac aagaagagct
     6421 aaagcaataa taccgtatac ataatctttc gtttgtgtcc attcatggaa gaaaacggct
     6481 cccgcaatcg tattcaaaat taattgaaaa ccagtagaca atggtagccc tacagaaaca
     6541 cccatgtact tcattccatg gaactgtcca ttttgtccaa cactccaagc taagccggat
     6601 aaaaatccga tcaagaaaat ccaaggggtg attacaggac taacaacgaa aaagacaact
     6661 aaagaaaaca gaaatgcacc gatcgtcatt cctagtgttt gttgattggc atcgccacca
     6721 attttaccac ttactaaacc aatactaccc catgccaaca tagggactaa tgctattaaa
     6781 atttccataa ccaatctcct tttatttcac atgataagaa acttatcagc gaaaacgtta
     6841 acaacacaag cacttttttt cgtttctaac gattttctaa cgtttttcat tataaaaatt
     6901 taagattggg aaaaataaag tcattgttgt cccttttcct tcagttgatt cagccgtcaa
     6961 atggatacct aatttttcgg ccagattttt tgctaaataa agtcctaatc ctgttgaatg
     7021 tgttttgcta agtcgaccat ttctcccagt aaatccctta tcaaagatcc gtttcaagtc
     7081 ttccgctggg ataccgatcc ctgaatcttt aactgaaagg ctgactcctt gcgcttcctt
     7141 ttctatgctc acaatgatgt ttccgtgatc cggcgtatat ttgatggcat tactaagcaa
     7201 ttggttgaag ataaaagcga tccatttagc atcggtcaaa acagtatgat cctcaccaac
     7261 gatctcgtag cgaatatttt tttggataaa ataatttgcc tgactgcgta tcacaggctg
     7321 aatgattcct tttagtccat attcttggat gaggtagtcc cgcgagaaac tgtcgagtct
     7381 tgcataatac aaaacttgtt ctacgtattc atcgattttt gataattcat tttctagtaa
     7441 tgtatacttt tgatcgtcaa tatcgaactc gatcgaacga actagcaatt tagaagcgga
     7501 aagtggcact ttgatttcat ggacccatga gtcaatataa tctttttggt cttgttgatt
     7561 actgattgct gattgcatta cttcttggtg ttcacggatc agctgattga tatattcttc
     7621 taccagtttt tcttcttctg tcctcccacc actcaaataa ttttgcaaag gggaagtttt
     7681 ttgtatgtcg gctaattttc cccaccatct tctcttgctc aagtaaccac ccagcagata
     7741 aaaaatcaga aagacgccct caatcaaagc taaataagca attgtgctca aatcaacttt
     7801 gatgtttggc gccagccaca tcacaaagat cgtcaatgca ataaaaaaca gccatcccaa
     7861 gatcaatgtc cactgatctt tcaaatattt aaaaaaattc atcttaaccg ctcatccttt
     7921 cagggaacga tatatccctg tcctactttt gtttcgatat atccttggat acctgcctgt
     7981 tcgatttttc ggcgtaaccg attgatgttg actgtcaatg tattgtcatc tacaaatcgt
     8041 tcgtcatccc acagtgctcg cagcaatttt tcccgactta agatctttcc attgtgcttc
     8101 atcaaaataa ataatagacg atattcattt ttgctcaaat caatgatctc tccgttgatt
     8161 tccatacttc cgttgtcaac attcaacgta atgccattgt ggctcatcat ttcactagat
     8221 aattctgtgt agttgtaaga acggcgaagt aatgcattga tttttgcaat cagtacatcg
     8281 atttggaaat gttttgtcac aaaatcatct gcccccatgt tcatagccat gatcatatcc
     8341 atattcgtat tcctgctgga gataaaaatg atcggtactt tcgatacttc acgtattttt
     8401 tgacaccaat aatagccatc caaaacggga agattaatat ctagtaatac aagctgtggt
     8461 tcttctcttt caaagtctga gaacacttga ttgaaatcgg tcgttccaaa ggtttcaaat
     8521 tgccatttct caagttcctc catgatcaat tgtcgaatga tctcttcgtc ttcgactacc
     8581 atgatttttg ccataaaaaa tccctccatc tttcttcttg ctactacaat atcaaagaag
     8641 cccttgtgaa acaagttggc ttacctggaa aatgtcattt tagcaaacgt taaaaaaaac
     8701 tggaaatatg cgatttaatc gcgtttgtct cttccaagca gcttaaattt attatgttat
     8761 tcttaataat tggtctatta tctgttattt ttctttcgtt gtttttgtct tttaaatttt
     8821 cgataacacc aataaccgaa aagcgttagc actagcaata tacctagaag gagtataagt
     8881 aatcgataaa gatggtattt ttttgtctgt tctatctttg aagagacttc ttctgtaaag
     8941 ggaactcgtc ttcctgtcac tagtaatcgg tgagtgttca cggtgtaagg tgtacaagtg
     9001 ataagtgtga ctagatcctg cttttctcta attgctaatg tatttgtttt tgttggaagg
     9061 acaacaattt tctcaacgac ttcataagcg agcatccgac catacacttc aagatagaat
     9121 cggtcatctt gctccatttc ttcaagatcc gtaaagagtt ttttctcagc aataccgcta
     9181 tgggcggtaa tcacggaatg tgtactctct cctccagttg gaaaagaggt gccttgcagg
     9241 actgtcgctc ctctatctaa caaaagatca ttcgtttcat caaataaagg caagctaaca
     9301 ttgattttcg gaatatagat ggctccgatc atatgttgtt cgtaatattc ttttcctgga
     9361 ttttggacat ttctaaccgc ttgctcaaaa ggatctttga cttgtcccat tcctgggatt
     9421 actgatgtat gagcaagcgt ctgatttttc ttttcatgca ctgctaattg ttgctcatgt
     9481 ttttcttctt tatcgcgttc aagctgcttt tgataattct cgatccttct ttgatccaaa
     9541 taattgctga gagcatctgc taaaaatgga tatgtaaaaa ctgcagctcc ggcaagaaac
     9601 atcactgcca taaataattg taataaaaga ctgatttggg tttgaggtct attacctatt
     9661 ttctttttca ttttcttcac cttcttattt aaaaaaactg cagcaacctt tataaaaccg
     9721 attgctgcag cttatttttg ttgcttatac ttcagacttc attttagatt tacggtacca
     9781 gctgtacgct ccaatcatca aacttattcc gattagtaag aatgccaaga gcccgttccc
     9841 accagtagca ggaagtgacc cgcgtttcat attcggtact ctttctggcc ctggattttc
     9901 tactcctacg atctgaattt cttcattata gcttccataa gagatgataa attcagtaaa
     9961 ggctgcctca cctgttggaa gtacataacc ttctggcgcc atggtctctt tcatttgata
    10021 ggttccataa tctaaccctt gaacctcaaa gtatccattt ccatctgcga caaattctgt
    10081 tgcttgtccg cttgttgccc actcaattct atctgctcct gattcataaa cagctaaagg
    10141 cgactcccct ttataaaata ttgcgtattc agaacggtct tcattccaca attcgaatct
    10201 tgcatcttta agcaattcac tgcttgatgc atcatatttt tcaaactttc ttccacctgt
    10261 tgtgactttc tctggcggct ctactccttc ttcataatca aattcatcat ttcgtccgat
    10321 ttcaataacc gctgtgttgt tatggaaatc atccgcttct agttctgtgg aaaccttcaa
    10381 attatacgtg atcgttaaat ctcctccacg tgcatctgct aatctaccta aagtatctac
    10441 tttggctact ccattttctt ctgtcaaaat cgtcacgaca aacccgttgc ttactacttc
    10501 tactatataa tcttgtcctt ttgttaaaat cgtctcgccg gctcgcactt ctatttcctg
    10561 atcataataa gttagaccaa ctgtaggcgt atctgtgatt ttgtaatttt tgataacagc
    10621 gcttccgttt tcaaggacag aatcgatgaa ataaggaata ggtgctgtga tcgtgtaaga
    10681 agccgttttt cctctttcta gatcaatata agaaatcgtt tcttgttctc cagcttcatt
    10741 aataatcgtt acttgtctaa ggtcgctttc ctctacattc atctgtttat ctgcttctct
    10801 gatttcgttt ttcggataca aatgcacatc tgtattgtat gtgtctttcc atgtagaacc
    10861 atcccacacg ccatccacca tatcaggcat catcactggc atcataacaa ccattggaga
    10921 cgcgattctg gaaatatgag ctggcgaata agtttctaag aaaagataga ctttatctct
    10981 agaagaaggt gcttcttccg tcacttggac ggtagagaat accgcttctc cagcttcatt
    11041 cgttgtttgt ttgtcaatac ttgtcagtcg atattttaaa aaccaacttt ctgtgtgccg
    11101 tttttgaatc cattcaatcg cttcggcaga attaagaccg tcttctcgtt ccggtgttaa
    11161 aggattatct cgaatcaaat cgtaatatac ttccgttacg tcaaacaaat caaaatcgac
    11221 tcctggaata ttttcttcac caaaatccgt agaaaccaat ccgctattcg gtcgttcttc
    11281 cggaacagat gagaaacctt ttttgtgtaa tgtaatcgtc acttcacttg gtttttgtgc
    11341 gacttcttcg gctttgacta gttttccgtt cccgaaaatc cctattcccg cagtaacgag
    11401 taacagtaca ctcattcccg tgacaatcat ttttttattc atttttttct ccccgttctt
    11461 tttttattaa taaaacagca cctaacaata aaagaattcc tagtaaagaa atcaacgttc
    11521 ttgcttcatt cgtttgcggg aatacggacc attttcctga ttgacctgga ttgcttggac
    11581 cactcggctc tttcggtttg tccgttgttt cttttacggt ctcgttataa acacgtggtg
    11641 ttgcggattc ataggcagag actggaactt tcccggattc taattcagcc atctcctgtc
    11701 cgttaactac tacatattgt ggttggcctt ttgcatccac ccaaaattca gggatgatga
    11761 cctcgattct cttacttgct tcatccatct cgtaaccttc cacacctttt aattcttcaa
    11821 aataataggt gccagaaggc aagaaacgtt gtcctgtcgt aacaaggccc tctttatccg
    11881 aaatgaattt gcttacattt ttgtcattca aaggatcatt gtctgctggt ttgatccatt
    11941 gatttttcaa gtcattagca ggagccatat ctaaataata tttcccccct tctatcatct
    12001 gatatagagc aaaaacagca ccttctaatg gttttccttt ttcagttgtt cctctttgac
    12061 gtccatactt gaagaagtat ggatctctta gataaccgac atttttagga tagatatgaa
    12121 tctcttgcag ttcctcggtt gattctgtcg ggtgataaat tggcaaaata gcagcaattg
    12181 ggatagctgt ctgatcgata tcaacatcca atccaacttc tggatcaatc gattcttcga
    12241 aaatcaaata aactgcatcc cgatcttggt ttttcttagg tgctttgata cgtgcaatcc
    12301 cgtctcctaa tccgccaacc gtatctttcc cagttgttac cgaagtccct atttggacaa
    12361 gcctttcttc tgtgattagc tgacggatat tgtttctgtt cgtttcagcg atttgctgga
    12421 cgaattcttt aggagtcatg ttttcagcag cttttttttc atgataataa tcggtcatgt
    12481 cgtaaatggc aaatgtcgct ccatttagtg gcaccgtctg tgaaatgata tctgctgttt
    12541 cccctgcttc ctcagagata gccaagccat cattttcatg gtattcaaat tgatcgttat
    12601 aatcgatatc tcgaatcatc cgtttatgca acacgaattc cacgacatct tcccctgatt
    12661 cttctgccga catcgcatgt ggaagaagga ctgctgcaag cggtagtaaa acaagtaatt
    12721 gaagcatcaa acacaacagc tttttcatta ccttgctccc tcctttttag attgtctgac
    12781 aaacaaacca taggctgctc ctgcaagcgc catgaagaat gcagcaagca ggatatagaa
    12841 taatcgtccg gatcctcctg tagcaggcaa cggattttca ggatcgttcc cgacactaag
    12901 ctgtaatctg attcgatttg tctccaaaag ttcatattct aactcttgct gtagatcagg
    12961 cgtctctgct tcttcgtttg gataatacga aatctcaact tttagtgtac ccgcttcttt
    13021 tcctggaact acccgatcat tttctatgtc aggttcttct cgataactaa tttctaaaat
    13081 aaagtaacct ggaagttttc tatagctgtc aggtgacttc acttccttga tccgatagac
    13141 cccttccaac aagtctgaag cattgaatag ggaagaatcg tttggatctg gtgccaagcc
    13201 tccctcgatg atttcatact ctcctgcact attttgtttc tcaattgaaa attctgcgcc
    13261 ttcaagacgt cggctattca tactgtcaat tttcaaaagt tccaaatcga ttggcgctag
    13321 ttcatcttct aaggttactt gtagtacacc atcgatgatt tcatagcctt ccggcaaggg
    13381 agcagtttcg tcttcgatga ccgtatcttc aatgataaac ttaccttctt cattcagtgc
    13441 aaactggaac acttgatcag atagccgata cccatttggt gcgacagatt ccgagacctc
    13501 atattcacca atagacagat gcagaggttg gtcgtaattt aaatcgacat acttttctac
    13561 tcctacggca cgagaataga agtatccgat cccaggcaaa cgtttcgatt cttctggttc
    13621 taacggatta ccagtgtatg ctcgatacag cgggaaggaa gaatccccca ctttcctgat
    13681 atcgaaacct gcaccatcta atgtatggcc ttcatggtct aattttctaa ctcggatctc
    13741 ccgataatca aacgtattgt aagcagtgaa tgctacccat aaacgatcct cttcatctgt
    13801 tgtatacgtg agatattgcc aattttcttc acctgtttgc ttgagttgga tagtccctat
    13861 agcattatcg taaagtcgca agatccatgt gtcattttca tcaggttctt tgaaaccttc
    13921 tgggttctgg ctctcaatga ttttgtattc tgcatagttg gtagtttctt cactatcctt
    13981 ataaacaaat gaatctggct ctccgattgg atgaataact ttctcaccat ccacgattac
    14041 atatttcccg tcttcatcta ctgaatagaa cgtgagtctc cctttctctt cctctacttc
    14101 gttcacacca gaaagccctt tcgctactgg atatccgatt tccagtccgt taccatctgt
    14161 ttcataaagt tcaaactctg cgccattgat tggcatatta ctgccttcaa ttagtttatt
    14221 gatattcatg tggattggct tgaattcatt tcgcatttcc agtttgacat acaactcatc
    14281 atactctgtg ccttctatgt taggctcttg tacaacagaa agcttgtgat attctccttc
    14341 ataataggaa ttcggatctt taccttccgg tccgccgact ctcgaaccag atgtatattc
    14401 atctacaagc tcgtagtgac ctaacatctc atcttcgttg gctgcatatt ttttgatttg
    14461 gaattgatac gtctcatcta ctaactgata acgtggtggt ccagcgatct ctctgatcca
    14521 aaatatgtcg ctgactgctc cagtttcatc tcctaaaatc ggcatcttcg tttcaaagat
    14581 cgctattccg tccgtggtag ttttacttgt ttcgattggt tcggcttccc cactctctgg
    14641 attttggtca tataatgcaa attcgactcc agccaacggc tcgccatttt gacccttctt
    14701 gctgacttgg aaattacggc ctttatgttc gtaacgaatg gaagaagatg ggaaatacat
    14761 ccgtccagta gtgatatcat tctcataatc ctccaggttc tgaatatagg ccatattgtt
    14821 cactgtgcaa aaatttccgt cttgagctcc cgcatcaagt tttacttggt acttgatcct
    14881 gaccatttcc ccttcaccta aagaaatcgg agaaaattta taagcttggt tttcccgtcc
    14941 gacatacgtt gtcggagtcg ccactacttc tggtggggct tgtccatgac gttcttcatc
    15001 ccatatttct ggctcttggt acttcatgcc gttttctcct ggatctagat ggtaaaactg
    15061 aatatctgcc gatctgccag ctccaaatgg tgagacatta aaaccagttt catcataaag
    15121 tgtggcttgc tggatcgttc tcttgaaggt agaagagata tcctccaagg ctgtaacaat
    15181 gtcattcttg ttgttcgcat tgtaatatcg cggtgttacg ccatcttctt ctaaatcagc
    15241 aatatttttc aatacatttc gtccaatagc atctagtcgt tgtctcccgc taacgtttcc
    15301 atcgattcct aaaccaatgg agaagatact tggcgggttc tcttgatact tgtttttcag
    15361 ccaatgatga taagcaactg tgttgatcgc agatgacttt tcttgcgaag taggactgcc
    15421 attcgtattg ttattagaag aaccatcacc aacccagtga gtcgctttat tatctccttc
    15481 ttttacctct ccataacgat agtgtacatt tacttcatct tctggacggt taaagtcaga
    15541 tgcataggta actggatgag taaaccctgc attagaagta ttcccagaaa actcctcaaa
    15601 atttctgaac aaaacgtcat tatcttcagc aatcttatct tccatcacag tccagttgct
    15661 ccaagctcca ttagtgctcg tacgataact tgactgaatc ggtgcataac tgaaggtcgg
    15721 tgtcccatct cccacaacga ttaggatttt ttctggattt ctattttctc cgccattgtc
    15781 tttatacaat gtttcatagc catttttaag ccctagtgta agcggagttc ctccgattgg
    15841 tgtacgattt aatgctgtat tctcacttcc cgtcaaacga ttgatatcat ttgtcaatgg
    15901 aaagatgtcc gaactgattt gctcatggtt attgatagct gtactattat ggtaaaaatt
    15961 caccataccg attcgaatat ccagactcgg gtcagagagc agatctttac tcatttcgtt
    16021 tactgcctca atcaacgcag catttttagt ttgactattg gtcccagcgg ttaattcact
    16081 catactcgct gacttatcta gaacaaaaac gatatcgatt ggactgattt cctttttctc
    16141 gcccccgatg acatctagat agacattgta aagggttgtt gcatcttcag gatgtagatc
    16201 agatgctgtg ctgcttggca gaacaggttc tacccatttt ttgatgtacg ccccgttata
    16261 atccaagtaa ctgttttcaa aattccgttc atttccccaa agctgaacgg tttgagctgg
    16321 aacgtttcta tcttcttcgc ctgcagttga tccattatta gtagcatagt cgtaatttcg
    16381 ccagttcgct cccccgtttg tataatcact atcatcgatc aagttctctc cgtcatatgg
    16441 cgagcctttg tctccttgat agcgttcatt gctttgtgtc caatttccta acacttttgt
    16501 atagtcattc gttgtaaact tcatctattc tttcatcaac caccacttga agaggtagtt
    16561 acccttcgtt cacatcgggg atcttttctg tttcaaaaat caatgtgccg gtttctgctt
    16621 cttttgaata aacttttccg acataccatt cttgaccatc gatcgtctcg acttttaggt
    16681 cttctccatc ttcatcatag gaggatagac ctgttccacg aacgtttttt acggtaccaa
    16741 tcccggcggc tgccgcatcg attgctaaac gtattctacc ttcattggca ctttcctttt
    16801 tttcaaaagc cagatgccat tcaagggttt cttctttttc aatatacttc ccttcaattt
    16861 ttgcatagtc ctcatctaag aatacctcat cctcctttgt ttctgttgac tcagcagcta
    16921 catccagggt cgtcatatga tagactgact ggaaatatgg tccaaatagc aaactgacag
    16981 ttactaactg caaaagcttc aattttaacc ctttcacatc cttatactcc tttcaaaaat
    17041 atttgtcttc tttcacaaca atcaacaaaa caatgcccat ctaattataa
//
LOCUS       DACEPD010000061        16529 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.064, whole genome shotgun sequence.
ACCESSION   DACEPD010000061 DACEPD010000000
VERSION     DACEPD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.064"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(141..416)
                     /locus_tag="IW797_001914"
     CDS             complement(141..416)
                     /locus_tag="IW797_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ1224692.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(659..1969)
                     /locus_tag="IW797_001915"
     CDS             complement(659..1969)
                     /locus_tag="IW797_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ1224693.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="IW797_001916"
     CDS             complement(2151..3380)
                     /gene="rpsA"
                     /locus_tag="IW797_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ1224694.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3515..4195)
                     /locus_tag="IW797_001917"
     CDS             complement(3515..4195)
                     /locus_tag="IW797_001917"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ1224695.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4244..4879)
                     /locus_tag="IW797_001918"
     CDS             complement(4244..4879)
                     /locus_tag="IW797_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1224696.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4945..6348)
                     /locus_tag="IW797_001919"
     CDS             complement(4945..6348)
                     /locus_tag="IW797_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ1224697.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6335..7366)
                     /locus_tag="IW797_001920"
     CDS             complement(6335..7366)
                     /locus_tag="IW797_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224698.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7397..7618
                     /locus_tag="IW797_001921"
     CDS             7397..7618
                     /locus_tag="IW797_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ1224699.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7783..8520)
                     /locus_tag="IW797_001922"
     CDS             complement(7783..8520)
                     /locus_tag="IW797_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ1224700.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8673..10013)
                     /locus_tag="IW797_001923"
     CDS             complement(8673..10013)
                     /locus_tag="IW797_001923"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ1224701.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10250..11773)
                     /locus_tag="IW797_001924"
     CDS             complement(10250..11773)
                     /locus_tag="IW797_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ1224702.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12151..12762)
                     /locus_tag="IW797_001925"
     CDS             complement(12151..12762)
                     /locus_tag="IW797_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ1224703.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12885..13010)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13092..13808)
                     /locus_tag="IW797_001926"
     CDS             complement(13092..13808)
                     /locus_tag="IW797_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ1224704.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="IW797_001927"
     CDS             complement(13814..14383)
                     /gene="scpB"
                     /locus_tag="IW797_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ1224705.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14367..15164)
                     /locus_tag="IW797_001928"
     CDS             complement(14367..15164)
                     /locus_tag="IW797_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ1224706.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15134..15505)
                     /locus_tag="IW797_001929"
     CDS             complement(15134..15505)
                     /locus_tag="IW797_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ1224707.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="IW797_001930"
     CDS             complement(15524..16411)
                     /gene="xerD"
                     /locus_tag="IW797_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ1224708.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4645 a         3577 c         2798 g         5509 t
ORIGIN      
        1 aaaaattaaa taaaatacat gtgttttcta tgtatgacat acggattcgg aatctctgtc
       61 tcttatttat tccttttctg ctaggaaata aaaagaaaag cacttgtagc cctagagcca
      121 caagcgcttt ttcaactgtc ttatttaaca gcgtctttta atgctttacc tggtttgaat
      181 gcaggtactt tgctagctgg aatttcgatt tcttcaccag tttgtgggtt acgacctttg
      241 cgagctgcgc gttcgcgaac ttcaaagtta ccaaaaccga ttaattgaac tttttcgccg
      301 ttagctaaag catcttgaat agttgaaaat acagcatcta ctgctgcagt tgcatctttt
      361 ttagttaaat cagtagctga agcaactttt tcgattaatt ctgctttgtt tgccatggat
      421 agattcacct cctccacaag agatatgata gaaatatcat ttagttaaaa ctttctgagt
      481 ggtccagaag tttaaaaaga aatattgatt aacttcaata ttctgttatc acgatatcat
      541 atgaaccgct taatagcaag gtttttacca attttcacat gcttttttta gtcatagaaa
      601 cgtaacaaac gaaactgtct ttttcttttg aaaaaaaaac gtcgatatgg taaaatgcct
      661 attttctcct acgagggata attcggatgg acgtcccttc aaaagtaaat gcttttctga
      721 tttgattttc taagaaacgt tcataagaaa aatgcatcaa ttcctcttca ttgacaaaaa
      781 cgacaaatgt aggtggtttg acagcgactt gtgtagcata aaaaatcttc aggcgttttc
      841 ctttatctgt tggtgttgga ttgattgcta cagcatccat aatgatatca ttcaacacag
      901 ccgaagagat acgaagattc tggttcatac ttacttgttc gatcatttca ggcagacgtt
      961 ccaaccgttg tttagtcact gcagaaacaa aaacgattgg agcgtaatca agataccgga
     1021 attcatcacg aatctcttgt tcgaaatcac gcattgtatt ggtttctttc tttacaagat
     1081 cccatttatt cacgacgata ataattccac gaccagcttc atgggcgtaa cccgctactc
     1141 gcttgtcttg ttcccgaatc ccttcttcag cattcagtac catcaaaacg atatcagaac
     1201 ggtcaatggc acgcattgca cgcatcacgc tgtatttttc agtattttcg tatacttttc
     1261 cgcgtttacg cataccagct gtatctatca ttaagaattt ctgaccactt tcagaaacga
     1321 agtgggtgtc gattgcgtct cgggtcgttc cttcaatatc cgaaacaatt acacgttctt
     1381 ctcctaaaat cgcattgatc aaagaggatt taccaacatt tgggcgacca atcaaactaa
     1441 acttaatcgt gtcgtcgtcc tcttcttcag tctcttcaga gaaatacttc actgcttcat
     1501 ctaaaatatc accaatccct agtccatggc ttcctgaaac aggataagga tcgcctagcc
     1561 ctaaggaata aaattcatag atatcgtttc tcatttctgg attatctact ttattgactg
     1621 ctaaaatcac tggtttatta cttctgtata aaattttcgc aaccatctca tctgcgtctg
     1681 tcacgccttc gcgtccgctg actacacaaa tgattacatc tgcttcttca atcgcgattt
     1741 ctgcttgatg cttgatctga tccataaaag gttcatcgcc aagatcaatt ccgcctgtgt
     1801 cgatcacgct gaattctcga cctaaccatt caccttttgc ataaatacga tcacgagtta
     1861 ctccaggagt atcttcgaca atcgagatac gctcaccagc gatgcggtta aaaatcgtag
     1921 acttaccgac gttcgggcgg ccaacgattg caattgttgg atttgccata ttatttcctc
     1981 ctcacaagtt tccaatctca cttagtttaa cgaatgcatt gccaactttc aagcatagat
     2041 tgtcaattaa acgaagtatt tttattcctt attgaaatac taatcgcttt tcaaaagaaa
     2101 aactgagaca aaagcagctg ctttcatctc agtcacagat acaaaatcaa ttatttttca
     2161 gaatcatcgg ttgctttttc ttctacttct tctttcaacg catcacctaa aatgtctccc
     2221 atagtaaatc cagtattttc ttctggtaat tcataagact caacattttt tggttcttct
     2281 tctgaagctg gtttttcttc taaagctttg atactcaatg caatacgatg ttcttctgga
     2341 tggatctcta atactttcac tttcacttcg tcaccttcat gaagcacttc atgtggtgtc
     2401 gcaatatgtt tatgtgaaat ttgagagata tggactagcc cttctactcc agggaacact
     2461 tcaacaaagg caccgaagct tgtcaaacgt ttaactgttc cgtctaatac tgtgccaact
     2521 ggagcttttg tatcgatttc tgaccaaggt ccaggctgtg tgtctttgat tgacaaggaa
     2581 acacgttctt gttcaggatt gatagacaac actttgacat ttacatcatc attgacagcc
     2641 aatacatcac ttggttttgc tacatgacta tgtgaaatct ctgagacatg gactagaccg
     2701 tcaattccgc ctaggtcgac aaatgcgcca aaatcagtaa gacgtgcaac acgtccactg
     2761 agaacatctc cctcatggat agttgaaaga atttcttttt tcttttcttc tctttcgttt
     2821 tccactaccg ctttatgcga taagatcaaa cgattttctg atggttcgat ttcgatgatt
     2881 ttaaagctca atgttttgcc tttatagtct gaaaagtctg caacaaaatg atcttccacc
     2941 attgatgcag ggacaaaacc acgtacaccg acgtcaacga ctaaaccgcc tttgacaaca
     3001 tttgtaacag gtgcttcgat gaccttgccc tcttggaaat ctctttcgat atcttcccaa
     3061 acttttttag catctaaacg gcgttttgaa agtaggtagc tgccgttttc tttatctttg
     3121 ccgatcgtac tgataacgac taaatctaat acatcaccga cttttactac ttcgctgata
     3181 tcttctaccg gcaaagttga taattctttt gccggaacga ctccttcaac tccagcccct
     3241 tcgattccga caatcacttg tttgtcttca atgactaaaa cttcgccctt aacaacatcg
     3301 ccaactttta cttcttgtac gcttttcatt gcgtcttcca ttgactggtt gttctcttga
     3361 ttttgatcaa attctgtcat aacacttgtc ctcctaacca acattctgcc gtaagtacgg
     3421 tactgttttt agtttaattg attattcaaa aaaaataaag cgatttcgtt cgttttcctt
     3481 gaaaaaacaa ttgtttttca ttttttttct taatttaaaa aagaagataa ccttttttca
     3541 aaacaacagc ttttattgct gcaacgactt ctggaatcga tttgccagtc gtatcgatac
     3601 gaacagcatc ctctgcttgc ttcagcggag agacgtccct tgttgaatca taatggtctc
     3661 tttcctgtat ctctttcgtc agtgtttcaa gatcagtcgg aattcctttt tcttggttct
     3721 ctttgaaacg tctttctgca cgctcactta cgcttgctac taaaaagatt ttcacttctg
     3781 cattcggtaa tacagctgtc ccgatgtctc ttccgtccat gacaacgcca ccgcgttccc
     3841 ctatttttcg ttggacagca actaattctt ctctgacttt tccatgtgca gagacttcag
     3901 aaacagcttt tgtgacatct ggttgacgga tttctaaagt cacatctttt gatgctatga
     3961 atacatgctg tccatcttct tgttgcgaaa aagagatagg atactttttg atcagcgaaa
     4021 ctagcccttc ttcatctgaa aaagatacat tgttcgtaat agctaaataa gttactgcgc
     4081 gatacatggc accggtatct gtataaatat aattcaattc ttttgctaat atcttggcaa
     4141 cggtactttt cccagatgaa gcaggcccgt cgattgcaat actgatttgt gtcatgatat
     4201 aactcctttt tctcagaaaa aaagccgcaa agcgcggcta ttattattta attcttaatt
     4261 cttgaccagg gtaaagcata aagttgtttg gatctaagcc attcaattca aacaattgat
     4321 cagtggtgat ccctgcacgc tcagccactt gttttggacc ttcgcctgct tgaacagtcg
     4381 ttgttgaacc ggctgcagct gatgaagaag gtgttgtttc tgctgcattg ctgcttgaat
     4441 cccctgcttg ataagttgcc tcacttgagg aagtcggtgt tgtctctgct gcaatactgc
     4501 tttctgacgt tacacctgta tcttgagaca cagtagattc ttccgcgatt gtactagatt
     4561 ctactgttga agtctcagtt gactcctctt ttgttgtact tgtagaactt tctactactg
     4621 atgtcgaagt ttcagaagaa gttgctgctg ttgcattgtt gcttgatcca tttctgatcc
     4681 agaaataagc acctgctgga atagcgatac ataagaccag taaaatcaaa aatagcgaaa
     4741 gaaataatgt atttcctttc ttttgttgtc tctgagaact tcttgatgaa acttcatcat
     4801 ctgtatcgta gattggttgt tcccatggtt cttgtgtttc gttattttct gtagattgat
     4861 gtctatcttt tctgctcact gttttacctc ctaaactcta tcgtgtgtat tataccatag
     4921 gaaaaagtgt cttgtcctaa aaaatcaatg ttccatgttt tttttaaata aatttagtaa
     4981 aatcgtttcc caatgaagtt gttcagtctt agaaaaaagc ttcctctttg gcagaagttc
     5041 tgcttttgct ccatcaatat cacaacaatt ctgcggcttt tcggagagtt tttcgccaaa
     5101 ataagctaag atgaattttc tgcggcagtt ttcttcatta atatagcgaa gcatctgctg
     5161 caggcggaat tctttttctt tttcctgacg tttgagccgt tccacccaat tttctggttt
     5221 ttcagactgc tggatcagct ccatccattt tttttgcagc tcatcaaagg gagcttgctt
     5281 tgtggcatat tccaaataaa gctcaaaact ttgtcgctgt tctctagaaa gttgattaaa
     5341 gaaataatgg atacgttcat ccccactttg ataaaaaaga attgctgcac tttcttcctg
     5401 atctctgcct gctcgaccga tttcttgtac atagttttct agactgtctg gcaaatcata
     5461 atggaccaca taacggatat cagatttatc gatccccata ccaaatgcat tcgtagcaat
     5521 gagaaactgc agctgatttt ttacaaactg ctgttgaagt tgtcttcttt gagctgcatc
     5581 aagaccaccg tgatagtatc caactgtaaa acgttccctg aataaatgat acaatcgttc
     5641 gacttctttt tttgttgcac aataaatgat tgccgctcct tgtgctcttt ccatgaagtg
     5701 ttcaagttct tgttcttttt gctgcgtttt ccgaacaaaa agcgagatat tttgccgatt
     5761 gactgattgt ctgatctctt ttggtggtcg atcaaatagt acatgacgta tatctcttgc
     5821 tactaaatct gttgctgtcg ccgttagtgc cagtgtgacc gggtttccta aaagctttcg
     5881 gatttttcct aactgttgat attctggacg gaaatcaact ccccattgag acacgcaatg
     5941 agcctcatct actacatata acgcaatatt ttgttttttc agatgctcta aaagagaagg
     6001 attagtcaac atttcgggac tcaaaaacaa aaatttatat tgcgatagat gacggaggac
     6061 gtatcttctt tcagattgcg aaagtaaact attgaaagca gctactcgtt tctcccctcg
     6121 tttttgtaat tgcgtcactt gatcttccat tagagaaagc agcggtgaca cgatgatgac
     6181 cagaccttcc attaaatacc ccgtcaattg atagcaaagg ctttttcccg ttcctgtagg
     6241 caaaatagcg agagtatctt ctccatcaag caacgcctga ataatttctt cttggccggg
     6301 acgaaatgaa tcaaatccaa aatatttttt taattcattt attaacattc tgtatcgctt
     6361 cctttctcaa tagatagatt tgatacaaac gaaattctcc ataatctaat tgtccatcta
     6421 cattccattc gctaaagcgc cactctcgca cggaatcctt atttggttcc aaacgttcga
     6481 ttgtttctgc agagagaatc cactcgtatg gaaaatcatc aaaaaacaag gcccactcaa
     6541 taagatgatc tgttactgtt cctcttttta aatgacggat tgccatgatc tggtcaattg
     6601 tctgaccatt cataaacatc tgcttggtga tcatcatgct ttgattgaaa ttttgctgta
     6661 ataatgaatc gatcaaagcg tgccaaagcg aatctggccg tttttcgatt tgtgctaaca
     6721 aaaggtggat acatctgctt tgttgcagat aaacctctga ctcatcattg ttttttgcca
     6781 gctgataagg aagcagccct gttcgctcat ttccagataa ttggttagct aaaaaatctg
     6841 ctgcttcttc tggtatctcc gaaaacagct gcaccaaact ctcatacgca gtatcaatca
     6901 aaatttcacg tggcaattgg ctttcactaa gccatttttt tagttggaaa gtataaaaag
     6961 gacttgtctc tgctggaaga tagtcttgaa tacccgttgc cagattagaa attacttgaa
     7021 cagcaaattt aatgagccgc caactagttt cccctgttct gccatagcgg tcaaaacgca
     7081 aaccagtatg ttctatttga tgttcggaca acatatcagc ccctgcaggc gttagctttg
     7141 cttcattttc atttatttcg atccaacctt gctgcgcaat tttttgcaaa atttgataaa
     7201 acttatcctg cttcaattct ggaaaactgt tatggataaa caaaagttcg tgggtaaatc
     7261 cataaataag tactgaagtc gttcgttttc ccacaaggag ttggtaaaga gaactaatcc
     7321 gcagcttgtg tcctgatgaa aatagcgata aaataaaaac aatcatatta ttctccttaa
     7381 gttaggatgg ttagaaatgt cactactatg taaacttgta cctgaacgct gtattgcatg
     7441 cggtctttgt caaatcgaag cgccagatat tttcgattat gatgaagaag gaatcgtttt
     7501 atttgcccaa gaaccagagg ctcatcaaca gttcgtgatg ccggaagaag aaacatatgt
     7561 aaaaaaagcg tatcaaaaat gtccagttcg agcgatttta ttagaaaata aaatataaat
     7621 ataaacgaaa ctttaaaaac gtacactaag ttctctaaac ttgaaaataa gccgggatct
     7681 gtttctcttg ttccgtgaaa tcccgactta ttttgaaaga ttcatttgct atctatagtt
     7741 aattgactag ttttttcacg gaccctatag gacccattta tattatttca actttttcag
     7801 ccagttttcc aatgtattga cgatcacagt ttgctggtct gcgttagaaa tttctgcctc
     7861 tccatctcct ttttgctcac catagctgcc aaaacctcca tgatttccgc cattgattga
     7921 tacatactct gttctttccg gtaaatactg cttagcttct tgataagctt cctgattcaa
     7981 tactttatct tttgtcgccg tgatcgataa aacaggaaca gctgattctg tcaatgttcc
     8041 ttttttgtcg ggataacttg ccatgaagaa tacccctttt aaatgtttgt ctgaatgatt
     8101 cttattgaca taacgacttg ccattacgcc acctaatgaa tggccaccga tcacataatc
     8161 ggaatttgga aaatcttctt tgaccttatc cgctttgttt ccgcccatca ctgctagatc
     8221 aagtggcatt ttgacaatag cgactgaaaa gccgtttttt gctaaatctt gcgcccaaat
     8281 actataactt tctggctcta cgagagcacc aggataaaaa ataagtaatg gagactttcc
     8341 atgattcttt ttctctcctt caaaaaataa gtaatcttct ttgttttttc cttcttttac
     8401 caacacttgc gcttctgacg atggttcgta cgtattttgc tgtatatagt aataaccggc
     8461 tccaccgagt aatcctaaaa tgatgactaa gccaattagt attcgtctaa atattttcac
     8521 tttctcccac cttttctatc tgagtgaccc ctttttctta atcaagtatg attatacctt
     8581 aaaaaaataa gagagaaatg caaactttcc agcatcaaat aaaaaaagac ggaatatcca
     8641 tttgaatatc cgcccttttt gtatgtttat ttttatttag cacataaacg atacacagct
     8701 tctgcataaa tttccattgc tttatacata ctacttaatt cccatcgttc attcgcctgg
     8761 tgcataaagt caggagtatc agggaacatc gcaccgaatg ccacacactg gttcatcgta
     8821 cgagcaaaag tagcgccgcc tgatacaaac ggttcagtca tgtctccagt aatatcgcga
     8881 tatgtcccca atagattttt cactaactca ctgtctagtg gcacgtataa tgaatcaagg
     8941 aagtcaaatt cttcataagt caacccatat ttttctgctg ttttagttaa tttagctact
     9001 aaatcatctt tcttgaatgt aacagggatt ctcatatcta caccgatttt tgtttgttct
     9061 ggtgtgattt ccaaacttgc aaagttcatc gttagctcgc cggattgttc atcttctgtt
     9121 ttgcccacga cagcttcacc agttgcattt tcttgaacta attttcctaa gaaattgatt
     9181 gggccaaaat caaatacttc tgaaagagca attgccagac gagaaatagc attcactcct
     9241 tgtgctgcat ctttcgcgtg gatactttta ccaagaacga cgatgccttc accttgttct
     9301 tcgaaatcga attcgtgttt tttcaatgct tcttttactt cagaaagctt ttctccagag
     9361 taaggagctg catcaggaac aacattcaac gctcctccag cttttacaga aaactcgttc
     9421 gttcctggtc ctgtcaagta agcttgcaat aagccttttt ctgcataaat taaaggaaat
     9481 tcagcatctg gcgcaaatcc ttgcgtaatt ccttcttctt tttcgttata tttttctaaa
     9541 caacgccata aattttcttc atctgtacca aagataaaac gaatacgtgt cttgaattcc
     9601 acgccagcat caatcaaagc tttgacggca tacattgccg caatagaagg acctttgtca
     9661 tcttgggatc cgcgaccaac taaccagccg tcttttatag ttggatcgaa tggttttgtt
     9721 tcccaattat tttcgtcccc tgctggaaca acgtccatat gacacaagat accgaataat
     9781 tcatctccag agccaatctc tgaataaccg taatagcctt ctggatcttt aaatgttcta
     9841 aatcctagct tttcactgat ttccaatact ttatccaaag cttcaatcac ttttttaccg
     9901 aagggatgtc cttgtcctga atctgcttca tctagtacgg aaggaattcg gatcaattcg
     9961 ctcaatgatt ctaatgcttg ttcatgatgt tgttctgtca caaatttttt catgatatta
    10021 tcctcctaat atgttaaaaa gggtcatgtc aaattcctcc gacacaacct cttcttctca
    10081 aataatgacg ttttaagcct gctccatcag caagcagaat gttcttaaca aatgatgaaa
    10141 gaaaaaacac tcactgaatg gagctattga cttgtccacg tgcttatcga tagccgttta
    10201 tcggtttctt cattcttatg atagaccttg tacctgtatt tgtctaggtt tagaacattg
    10261 ctgctacacc taggatcaca cagatggcta caaataatat cgcaattaat ttaccggtaa
    10321 atttccacca cactgaaaca tcgaaacgag caattgctac agcacccatt acgatagcag
    10381 atgttggtgt aattaagttt actaaaccag atgcagattg ataagcagtg attactaaat
    10441 ccttaccgac gccggcaaat tcacccatag gtcccatgat tcccattgtt gcagcagcta
    10501 agccggaagt tgaaggaatc aagaatgaca tagggatata gaaaataaat gtaataatga
    10561 tgaatacact tgaagataat gaacttaatc cttgttcgcc ccaatgtaag atggtgtctg
    10621 tgattaatcc attgttcata accacttgga taccacgagc aacagctact acgattgcta
    10681 caccgatcaa atcagaagca ccagctaaaa attcagagac aaattcagac tctttcatgc
    10741 cataagtgat tgctactacg atggacatta ctaagaataa catagtaatt tctgggaagt
    10801 accagtcacc taatggaagc atgcttttac ctagaaccgt acctagtacc ggaaggtttg
    10861 ttaaccagtt tgtcaatgat tcaaaaatcg tgaaattcgc attcaaggtt gtccaaggaa
    10921 tcaaactgat gatcatgata ccaaaagtaa taaagaacaa tacattaaca tgtttttgtt
    10981 ttttcgtgat tacttcttga cgaccgattg cttcaatatc gaaatgcttc atatcttctt
    11041 tacgttggtt gtatactaat gattttgttg gatctttttc gatctttgat gcataacgat
    11101 acacatatac aatagaaata gctaacaaaa taacgaacat caagaatcga agaccaatac
    11161 cttcacccat cgaaatccca acagcttgtg aagctacgcc tgttgcaaaa ggatttaccg
    11221 ttgaagctag acagcctact tgagacccta ctaatacgat tgctacagct actactgtat
    11281 caaagcctac agccatcatg actgggatca gtaaagcata gaatggaatc gtttcttctg
    11341 ccattccgta agtagagccg cctaatgcaa ataacagcat caatactgga ataagcattt
    11401 tttcttttcc tttgaatttc ttcacaactg aagcaatccc ggcatctagt gcccctgttt
    11461 tgttgataat acctaaaaat ccaccaacaa ccaaaatgaa gaaagagatt gggattgcag
    11521 caggtgtttc tttagttagc tcccccgctg gtacacctag catcccattg attggcgcca
    11581 tgaaaatatc ccacagccct tgaggatttt gttgtactcg ttcatatgat ccagcgacga
    11641 tgttgcctgc atcgtctaca ctatagtgcc cagctggtat aaaccaagta agtacagcga
    11701 ttactgcaat aataataaat aagaccgtat acgctgaagg catttgaaat tttttctttt
    11761 ttgtttgttc catcttcttc ttcctttctt ttccgttaga gacaacaatt ttggctctaa
    11821 tgttatttta tcaaaaagag tatcagaaca aaaataataa cacgcatttt gtaaaacttt
    11881 tgttcaaaaa gttcgttttt ccctattccc tcctcttttt tcaggttttt aaagctttct
    11941 tctttttcat tgtagcgttt cctttttttt ataaacaaac attttcgtct tttaaaagga
    12001 gaagaatttc acatttgcaa agaattgatt gatttttatg cataaatcaa aaaaatactc
    12061 aaaacaaaca aataaaacgt ttacattatt gcatgtcttg tatttttttc caaaaaaaaa
    12121 gaccaagaaa tttcttgatc tttttgatat ttatttgact gctgtatgac gttgttcgaa
    12181 ctgatgttgt tttcgactta accatggaag aagttgcgca tgcagcacta aaaagacagc
    12241 actcacgatg aatcctttga tcaagttgaa aggaacgatc ccaatcagta catagtttgc
    12301 taaagacatg cccagcatct gtgttgcgtt cagccctaaa aacattaagt aaagaggcgt
    12361 aataacgaaa taatttgcga tactcataaa aatagttaga gcgatggttc ctgtaatgac
    12421 acctaaaaat ttgtttcttc ctttatgctg tttctttaca ctaaagaaat aataaatcgg
    12481 gaatgtaaag atcgttgttg ataagaaact agcagtgtct cctaccaaat tatccggaga
    12541 aaaacctgta gtcagcagat gtaatactga acgcaagaac gctgttgcaa ctccggccgc
    12601 tggaccgaat aagaacatgc tgatcaatac cgagatatca ctaaaatcga tcttcaaaaa
    12661 actaaaagcc ggcatgatcg gaaaagcaaa atactgtaaa actaatgcga tcgcagcaaa
    12721 catcgctact gcaaccattt tctgtacacg ggtgttcttc atttgaaatc ctcctgttag
    12781 gactcctctt cagcgaactt tttgacgttt ctctccaaat gaaaaaactc tattttccag
    12841 ggaaaataga gttagagtta tttgatcaag cgtcgtcaaa taagctctat cttctttatc
    12901 cagactatac tgtcggtatc ggaatttcac cgattcagct atttttttgc aaaataggtc
    12961 gtggactata accaccggtc gggaatttca ccctgcccct gaagacgaac cttatataat
    13021 ttttgtttac atattcatta tacatatcta ttcaaaaaac gcaacatcta acacttataa
    13081 aaagtaagtg ttcattttga gtggttcagc aaactgctga cttctttttt gttcagttca
    13141 cggtaatccc caggacgtag gccttgtaaa gtcagatctc cgtatttttc acgttttagt
    13201 ttttgcacag gaagaccaac tgcttgaagc atatttttca cttgatgatt acgtccttcg
    13261 tggatcgtca attccacgat actgtggttt gtcttaggat ccactgataa aatttggaat
    13321 ttagctggag atgtttttcg tccttcgatc tttattcctt ttgtcaaagg tgcaagcatc
    13381 gattttgtag cgatcccttt tacctttgca acgtaaactt tatccacttc atgttttgga
    13441 tgtgtcaggc gctgagcaaa atctccatca tttgtcagta gcaagatccc cgaagtatcg
    13501 taatccagtc gacctacagg atagatacgt tcttttacca tcggcaaaag atccgtaaca
    13561 acttttcttc ctttgtcatc tgcaacagca gaaataactc ctttaggttt gtacaataaa
    13621 tagtaaccgt attcttcttg ataaattggg acttcatcca cttccacagt gtcatgtttc
    13681 ccaactttta tccctaattc ccttacaact tgtccgttta cttttacacg tcctgcaagg
    13741 atgaattctt ctgcttttcg acgagaagtg atgccagcgt gggcaattac tttctgcaat
    13801 ctctccatta caattattct cctttattca ttccatcaaa gaatagatct ggtgacacgt
    13861 cttcttcaag tgaaatttcc atttcttgtg tatcaggcag ttcttctaat gatttcaaac
    13921 caaaataatc taagaagtag ccagttgttc catataatat cggccgacct ggtccttcta
    13981 ctctaccttt ttcttcaatc agtttatggg cagctagacg ctgtaccgct cctgaaacct
    14041 gaactcctcg aatctgctcg atttcagctc ttgtgattgg ctgtttataa gcgatgattg
    14101 ctaaggtttc gacggcggct tgagataatg tgtttgatgt cccttgcgca tatcgcttaa
    14161 gtaagggagc cagctctttt ttagtcgtta atacataatg gctttccgtt tcaaaaatat
    14221 gcaaagctga gtgcgagttt tgagtgtaag attcttttaa ttctttcact aaacttgtga
    14281 cttctgcttc gctgagattt aacagtccgc ttaattcttc attactgatc ccttcctcgc
    14341 caacaataaa aagaagggct tcaagttcac ttaatttcgt catgttcggt atctcctact
    14401 gctggataaa gcataattga ttcatagttt tctttttgtt gcgcaatgat ctgccctttt
    14461 ttcatcaatt ctagcaaagc cataaaagta gtgacaactt cacttttaga aggtgactca
    14521 aaaaaagaat cgaaagtaca gccatgcgca tttcttttta tacgctcttc gataaatacc
    14581 attcgttctt cgatcgttgt gtcatcgggc gtgatcgttt tttccacttt ttgagaaaat
    14641 ttcttacgtt ctaagatttt ctgcatggca cggatcaaat cgattttttt gacttgtcct
    14701 cgcttcaacg gcttttcagc atccgctaac ccatcgatgt tcataggttc ttttgtgtaa
    14761 taaagacttc gttcctctgc tttttctgtc agtctttcag ctgcatattt atatttacgg
    14821 tattctaaaa gctgagtgac caattcttct cgaggatctt cttcccataa ttcttcatcg
    14881 tctgtgccga attcttggac aggaagcaat gtcttgcttt tgattgccat caatgtagca
    14941 gccatcacta agtattctcc tgcaacagct agctcgagtg ttttcattgt atggatataa
    15001 ttcatatatt gctccgtgat cgtagcgatc ggtatatcat atatatcgat ttccatttgc
    15061 tgaatcagat gaagcagcag atcgagagga ccctcgaata catctaattt aatattgatc
    15121 tccgtcaact cattcactct ttcttctttt tctttgctag ccattttcca atgtaaggct
    15181 ctgtgatttc agttgcacgg atggagacat ccgggtactt ttcctgtaat tcatctaaca
    15241 tttgaaagcc catattctca ccgcgataag acggattcaa agaaatgtca tggatgatca
    15301 gctgatcgtt cgttgcccag taaataccaa taatcccttg aatgttggtt gaaccttctg
    15361 gcgtaaagaa aaaaagttca taatcgtctt cagattcgta cgtatcaatc tccttcaaca
    15421 aagaagtttt acctgtcagt ttcttatgga aactcaatag tcccatagca attttacggt
    15481 tttctttccg gtaatgtgtt aacatcttct caccttccta tgactaagcc cttggaaaat
    15541 gctgcttata aacatccgtc attcttttct tcgtgatatg tgtataaatc tgagtcgtag
    15601 agatatccgc gtgacctaac aattcctgta ctgtccgcaa atcagctcca ttttctagca
    15661 gatgtgtggc aaagctatgc cgcagcgtat gtggtgttac ttctttgtaa atcccagcct
    15721 ctcgtactaa ctgcttcaga tttttccaga ttccttgccg agaaagacct ttcccatgat
    15781 tattgacaaa tacatgcatt tcagatgcgt cctttacaag taatggacgt gcttcgtcta
    15841 agtagcgttc cagccattgg atcgcataat cacctaaagg aatgatccgc tctttatctc
    15901 cttttccaac tgtctgtacc agtccaagag aaagatgaag atctcccaat ttcaatccaa
    15961 tcaattcact gacgcgcatc ccagtagcat acatgacttc caagattgca cgatctctta
    16021 tccctaacgt ttttgtcgta tcaggtgctt caatcagctt ttctacttct gtcagagtca
    16081 atgtacttgg taatttctgt gctttcttcg gcgtatcgat atgttgcatc ggatcatgat
    16141 cagtgatacg ctcttgccgt aaaaactgat ggaatcttct gagacttgag atcatccgag
    16201 taacagttgc tgtcgctttt ttttcttcat gcaacttctc cagaaaacgg atgactaaaa
    16261 agcgatcgac atcctgccat gaagcaattt tctgctcctg tagaaaatgc aagtactgtt
    16321 ccaaatcacg ttcatagctt tgtcgtgtat tggcggacaa tccccgttca atattcaagt
    16381 agtggagata atcgttgatt tgctctttca taataatccc cctcatgcct ctctatcata
    16441 tcagataccg cttcattctg atagtctttt tccataatct gagcaaagaa aaagccgtct
    16501 agacactcag acggactggt ttctttttt
//
LOCUS       DACEPD010000062        15690 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.065, whole genome shotgun sequence.
ACCESSION   DACEPD010000062 DACEPD010000000
VERSION     DACEPD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.065"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(12..482)
                     /locus_tag="IW797_001931"
     CDS             complement(12..482)
                     /locus_tag="IW797_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ1224709.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="IW797_001932"
     CDS             complement(641..1099)
                     /locus_tag="IW797_001932"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ1224710.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="IW797_001933"
     CDS             complement(1270..2415)
                     /locus_tag="IW797_001933"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ1224711.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="IW797_001934"
     CDS             complement(2428..3345)
                     /locus_tag="IW797_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ1224712.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPNGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="IW797_001935"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="IW797_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ1224713.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4822..6081
                     /gene="lysA"
                     /locus_tag="IW797_001936"
     CDS             4822..6081
                     /gene="lysA"
                     /locus_tag="IW797_001936"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ1224714.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6068..6868
                     /gene="dapF"
                     /locus_tag="IW797_001937"
     CDS             6068..6868
                     /gene="dapF"
                     /locus_tag="IW797_001937"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ1224715.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6871..7752
                     /locus_tag="IW797_001938"
     CDS             6871..7752
                     /locus_tag="IW797_001938"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ1224716.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7749..8465
                     /locus_tag="IW797_001939"
     CDS             7749..8465
                     /locus_tag="IW797_001939"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ1224717.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8471..9166
                     /gene="dapD"
                     /locus_tag="IW797_001940"
     CDS             8471..9166
                     /gene="dapD"
                     /locus_tag="IW797_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ1224718.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9182..10183
                     /locus_tag="IW797_001941"
     CDS             9182..10183
                     /locus_tag="IW797_001941"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ1224719.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10180..11541
                     /locus_tag="IW797_001942"
     CDS             10180..11541
                     /locus_tag="IW797_001942"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ1224720.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11501..12652
                     /locus_tag="IW797_001943"
     CDS             11501..12652
                     /locus_tag="IW797_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ1224721.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12627..13796
                     /locus_tag="IW797_001944"
     CDS             12627..13796
                     /locus_tag="IW797_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1224722.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            13971..15350
                     /locus_tag="IW797_001945"
     CDS             13971..15350
                     /locus_tag="IW797_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1224723.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         5123 a         2767 c         3340 g         4460 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aaccattagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt
     4681 tattcttatt tttaaaaaaa tatttcgaaa tatactagac aattcagaaa attttgttta
     4741 atattattcc aataaaattc aaatctttat caaatgaaat tccaataaaa acggaaaaaa
     4801 agtgaaaaga ggaatgaaaa aatgacagaa ttgataattg gcggagtcac agcaagcaca
     4861 ttggcagaaa agtataagac acctctgtat gtgtatgatg aagaaaagat gagacaaacg
     4921 atgctgtcat tcaagcgagg ctttgtatcg aaacgttttg atacaaaagt gctttacgcc
     4981 tctaaagcat tccaaacgat tgagatgctg catttagttc gtgaatatgg attcgggcta
     5041 gatgttgtca gtggtgggga aatctatacc gctttgcaag ctgattttcc tagagatact
     5101 atctacttcc atggtaacaa caagacgcca gaggaactga tctatgcact tgaaaatgat
     5161 ttgttgcata ttgttgccga taatttgatg gaagttgaat tattagcaga attaagccaa
     5221 cgctatttaa aaaaaatttg tatcatgctt cggttgaatg tcggagtaga agcacatact
     5281 catgaatata tcgtcactgc gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa
     5341 gagtgccaac aagcccttca gttgataaaa gaaagcagct atctagagtt agaaggattt
     5401 catgctcata tcgggtcaca aatttttgat gttactgctt ggttagctga aatcgacaaa
     5461 ttgacgaatt acttaaccga ttttgaccaa cctctttcct tgaatcttgg cggagggttt
     5521 gggattcgtt atacacaaaa tgatcagccg atcccagtcg aagaggcact aaaaaaactg
     5581 attgaatata cggaagaagc tttggaaaaa cgaaaactga ccattcgtca attactgatc
     5641 gaaccgggta gaagtattgt tggagaagct ggtacaacac tatacacgat tggtttcatc
     5701 aaaaagacac cacataaaaa atattacttt gtagatggcg ggatgacaga taatatccgt
     5761 ccagctctgt atcaggcaga atacgattgt gatttagcag caaaactgtc ggaagaaaaa
     5821 acagagaaag tgacgattgc aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa
     5881 gcttatttgc cgaaagcaga atcgggtgat cttttagctg tttattccac gggagcttat
     5941 ggatactcga tgagcagcaa ctacaatcga gcgacacgac cagcagttgt ttttgtcaat
     6001 aatggccaat caagactgat tgttcgcaga caagcatttt ctgatctact aaggggggaa
     6061 gtttcctatg atccttcata aatataatgg ctgcggaaat gattttatct tattagatta
     6121 cgcagaagac acagattata gtcaattagc cgcttcttta tgcacaaaag aaaaatatga
     6181 tatagacgga ttgatcgccg taaaaatcga tccgttggaa atgatctatt acaacaaaga
     6241 tggcagccgc gcgccgatgt gcggaaatgg cattcgctgt tttgctcgat acgtaaatga
     6301 tcagaacatg atgaatgaac aggaattcaa cgtagagaca ttagcaggca caagaaaaat
     6361 tacggtgttg gatccctcac ctttttattg cagcgtagat atgggagtgc cagatgattc
     6421 tccggaaaaa atcaaagcta atcaaactta tccaataaaa aaccaggaaa tagagatcaa
     6481 tggtgaaaaa gtaacgatca ctagtttatt tttaggaacg attcataccg ttgtttttgt
     6541 tgaaaatgcg cgtgaggaag tctggagaaa aagaggtgaa ctgatctgtc atcatccttt
     6601 gttcaaagaa aaaaccaatg tcaactttgt agaagtagtc aatgaacaag aattgatcgt
     6661 ccgtacctat gaaagaggag taggttggac acttgcttgc ggtacaggtt gctgtgcctc
     6721 ttatgtggtt gctaaggaaa ctgggaaaat ttcagcagat caggtagttg ttcacttgga
     6781 acaaggagac ttgatgatct ctggtgagga tgcgatacaa atggctggcc cagcagttca
     6841 tgaatggaca agaatattgg aggaatgaag atgataaaag gttcgatcgt ggcgctgatc
     6901 acgccgatga atgaagaggg atcagtagat tatgctggtt tggaaaaatt gatccaattc
     6961 catttagatg aacaaacaga tggattatta gttttaggta caacaggaga atcttctacg
     7021 ctcacacaat ctgaggagga gcagatcctt caactgacag taaagaaagt ggctggccgt
     7081 gtgccggtga ttgcaggagc tggaacgaat aatacgaaag aaacgattga aaaagcgaag
     7141 cattttgctt ctttgggggc agacgcactt ttggtcatca ccccttacta caacaaaaca
     7201 agtgatgcag gactagctgc tcattttaca gcaattgcag aagcctcacc gattccgctt
     7261 attttgtata atgttccttc tagaacaggc atgtcgattc caatccatgt attagtaaat
     7321 ttagcagaac atcctaatat cattggtttg aaagaagcct caggagatat ggcttatgtg
     7381 atggatgccg ctcggttgat tggagaagaa ttcttccttt acagtggaaa tgatgatttg
     7441 atcctaccag taatgagtgt aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa
     7501 cctaaaatag tccatgaatt agtaaaagat acgcaggacg gaagatggca gcaagcaaaa
     7561 gaaaagcaat taaatgcact tgaactgatc catgccttgt tcagtgagac caatcctatt
     7621 cctgtcaaag cagccatgtc attattagac ttaccttccg gacctctacg cctgccttta
     7681 gtttctcttt caaaagagaa aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg
     7741 gttgaaaaat gaaaatcgga ctgattggca gtggacagat gggaaataga gtcgaagaag
     7801 ttgcacaaga aagaggagat acggtccttc ttttacggac ggcacctaaa gaagcaacta
     7861 cgcttgcttt tacggaactg ccagaactgc ttattgattt ttctcatcca gataatttag
     7921 agaaaatcct ttcgtatagc ttatcatatc aacttccgtt agtcattggc acgacaggtt
     7981 atacacaatc acagttccaa gaaataaaag aacatgccaa acatgtacct gtgatgtata
     8041 gtgctaattt ttcgttcggc atcatggtga tgaatcaact aatcaagcaa gcagctgcaa
     8101 tgttgcaagg ctggcagatc gaactgattg aaaaacatca tagtcgtaaa aaagatgcac
     8161 catctggtac tgcaaatatg ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg
     8221 tatacaactg gcaagggaaa aaaagagaag aaaaccaaat tggtgtacac agtattcgtg
     8281 caggttcttt acctggtgaa catgatgtat tgtttgctgc tactgatgaa atatttacga
     8341 tcaaacatga atctttttct aatcgaatct ttgctgcagg cgcagtagaa gcagcggact
     8401 ggttgaaaga tcagtcacca ggttattaca aattagaaga tttattaatg gacaaagggg
     8461 tgtaaaaaac gtggaagctc aagaaatcat ccgctacatt caaacggcat ctaaaaagac
     8521 gcctgtgaaa gtctatctga atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt
     8581 tggtgaagga agcagcttcg ttgtttttgg tgactatgag acgattgctc cggttttaga
     8641 agctgaatca gaaaaaatca ttgaaatcga aatcgaaact gctgcaagat acagcgcagt
     8701 tcctttgcta gatattaaaa aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga
     8761 tcaagtgtca atcggcaaca atgccgtgat catgatggga gcgattatca atattggtgc
     8821 agtaatcggt gaaaacacga tgattgatat gggtgctgtc ttaggtggac gagcgacagt
     8881 gggcaaaaac tgccatattg gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc
     8941 ggccaaaccg gttatcgtag aagacggcgt actggtaggt gcgaatgcag tgattgttga
     9001 aggtgtccat atcggaaaag atgcagtcgt ggcagcagga gcagtggttt tagaagatgt
     9061 ggcagcagaa actgttgtcg gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca
     9121 gacaaaagaa aatacagcat tgattgctgc gttacgcgaa ttatagagga gatggagaaa
     9181 aatgcgcgaa atagatacct gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct
     9241 caaagtgtta gaagaatatg attttccagt aggacgattg aaattattag cttcaaaacg
     9301 ttcagccggt aaagtttgtg tattcaagaa aaagaaacac actgtacagg aattgacaga
     9361 aacttcattt gaaggatatg atttagcgct attttctgca ggtgcttctg tatccaaaga
     9421 gtttgcgcca atcgcacgaa gtcagggact atttgtcata gataattcct cagcatggcg
     9481 ggaagattcg gatattccat taatcgttcc agaggtcaat ctggaagact atacattgaa
     9541 tcggctgatt gcaaatccaa attgttcaac cattcaagcg gtacttccgc ttaaagcttt
     9601 acaagaagcc tttggtatcc agcgagtcaa ttattcgacc tatcaagcag tttctggttc
     9661 cgggcaaaaa ggcattgaag atcttcatcg tacgcaaaat gacgaggaac cagttttgta
     9721 tccttataat attagccaaa cggtgattcc ggaaatcgat cagcctttgg aaaatgggta
     9781 tacaaaagaa gaacagaaaa tgataaatga gacccgtaag attctgcatc agcctgcttt
     9841 gccagtatca gcaacctgcg tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc
     9901 agttcaactg caaaaagagt ttacgacgga agaaattaga gagtgtctca gccagtttcc
     9961 agggattcaa ttagaagatg atctagcagc acataaatat ccgacgagca ttcttgctag
    10021 aggaacagat atggtttatg ttgggaggat cagaaaagac acttctttag agaacggatt
    10081 gttgttctat acgactgccg ataacatacg caaaggagcc gctgcgaatg ccgtacagat
    10141 tgctgcagct ttaatgcaga aaatagcgga ggtggcaaga tgagtaatac gctgaaagta
    10201 gcgaaattcg gcggaacatc gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt
    10261 gttctttcag atcccaatcg ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca
    10321 gatgatgaca aagtaacaga cttactttat ttgatttatg cttaccttca gcatcatgtt
    10381 gattggacac ccctttggaa aaggatttgt gatcgtttca ttcaagtaag agatgttctt
    10441 gccctgcagt tacctattga agatgaacta gcacgcatac agaaacaact ggaagacgaa
    10501 aagataactg aagattattt agtaagtaga ggtgaatatc tgactgctca actgatggct
    10561 gagtatcttg gttaccagtt tattgacgca aaagacctga ttttctttga ctatgatggg
    10621 aaaatcgatc aggaaaaaac agcacaggaa atcacaaaag catttaaaag atatccgaaa
    10681 atggttgttc cagggtttta cggggcgaac cctgcaggaa aagaaaaatt attgggccgt
    10741 gggggaagcg acattaccgg cgcgatactg gcgaagatgt tgcaagcgga aagttatgag
    10801 aattggacgg atgtttcagg aatcatgatg gctgatccgc gaatcatcga tcatcctaaa
    10861 atcatcgagg aaatctcttt tagagaactg agagagatgg cttatatggg ggctaacgtt
    10921 cttcatgaag cagctgtttt cccagttcaa gaggctgata tccccatcca aataaaaaat
    10981 accaatgatc ctttcgctca agggacaaaa atatcgaatc acgaaataga aaaggaaaat
    11041 ggactgacag gaatagctgg acgcaaggat tttctttcca tcactttatt caaacgtcat
    11101 atgtccgatg aaatcgggtt tatctggaaa gcaatgagca tttttgctaa acatgagata
    11161 tcaatcgaac atattccttc agggatcgac aatatcggag tgatcgtctc agcagaagcg
    11221 attgccgata agttatttct catcacgaaa gaattaaaag aaacacttgg cgtggaagaa
    11281 atcgtcgtga tcgaagactt agctttgatc tctgtcgttg ggggtccgca taaagaattg
    11341 attggattat ctgggaaagt gttatcgata ttgaacaaat tagaaattcg cacatcgatc
    11401 ttatcacaag gagcacagga gttgaatctg atcatcggtg taccgaataa tcaatatgaa
    11461 acagtagtga agggtatcta tgaagggatg gtgaatacgg atgctgaaag aatatcgaat
    11521 ggcactccat caaattcctg aaatcgggtt tcatgaattc aagacaaaaa aatatttata
    11581 tgaacaagta aaaaacagcg aggccgttat ccatgaaatc ggcgaaacag gactactcct
    11641 ttattttgat aatcagcaag aagaaacgat tgcttttcga acagatatcg atgcactgcc
    11701 agtcaaagaa gaaacgaatc tctcttttgc ttctaagcat gaaggttata tgcatgcatg
    11761 tggtcatgac ggacatatgg cgatgctttt agggttgact gactttataa ctaaacacat
    11821 tgaggatctt atttataatg tagtactgat ctatcagcca tcagaagaaa tttccggtgg
    11881 cgcacaaagc gttattcagt caggattgtt ggataaatat gaagtacaag caatatttgg
    11941 ctttcatcta tggcctggac tagctaaggg agaagttttc tctcgaccag gacctttgat
    12001 ggcacaaagc agtgaaacag acattatcat taaaggccag tctgcacata ttgcttctag
    12061 tgaaaaagga atcgatagtc tggaggcagc tgtacgattt ttgaagaatg tttatgattt
    12121 tgaccggcaa ttaccggcag agtggaatca tctaatgaaa tttggccaaa tccaaggtgg
    12181 aacgatccga aatgttttag ctggagaagt tattgtatcc ggaagtatcc gttcttacag
    12241 ttcagatatg caaaattatt tgaagagggg attagaccat ttagcagaac agtttcactc
    12301 tacagctact atttcttttc gctataatga cggctatcca gctgtaatca accatagagg
    12361 cttatatgca gcactgttag attcaaacaa actacatgaa ttagatgctc cagttcttca
    12421 agccgaagat tttggcatat atacgcaaca atatccatgt gtatttttct ttttaggtgt
    12481 cggtgatacc cctgctttac atgatccgac gttcgatttt gatatggatg ttttagaaaa
    12541 tggtgtagaa tggtacaaaa cgattctttt tacagaaaat cttcttgtaa aaatcgatag
    12601 cagttcaaaa gaggagcgag taaataatga tgaatttatc caatcgattt agtcctaggc
    12661 tttctagaat agaagtatca aaaatacgac aattcgatca gcaaatatcg tcgataccag
    12721 atgtgatcaa actcaccctg ggagaaccgg attttccaac tcctgaacat gtcaagcaag
    12781 caggaatagc agcgattgaa gaagactttt ctcactatac cggaatgaga ggattagagg
    12841 aactgcggga agcggcttgt atatttcagc aacagcgtta tggattaact tatgatccgc
    12901 aaacggaagt tttgacgaca gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg
    12961 ttttggaaga aggagacaag gttcttattc cagcgcctgc ttattcagga tatcagccat
    13021 tagtggaact agctggtgct gaattgatcc cgatagatac ttctgacaca gggtttgtct
    13081 gtcagccgga acaatttgaa cgagcatttg aacaatatgg atcagctgtc aaagcagtca
    13141 ttttaaacta tcctaacaat cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa
    13201 tcgctgaggt tttaaaaaaa tatccggtat ttgttataag tgatgaagta tatgctgaat
    13261 tgacttatag tggaacgcat atgtccatag ctacttatct tccagaacaa acaatcgtga
    13321 taagcgggtt atccaagtca catgcgatga ctggttggcg agtgggcttt atttttgctc
    13381 aaaaagccct tattgacgaa ctaattaaag tccatcaata tctggtgact gccgctacaa
    13441 cgatgtcgca aaaagctgcg gctgaagcgc taataaatgg ggtgaaagat agcgagaata
    13501 tgaaagagca atatcaaaca cgaagagact atctggtcca gcaattggcg cctttaggat
    13561 ttgaagtgac ccagccaaac ggtgcatttt acttgttttg caaactccca gctgccattc
    13621 aaactgactc ttggcagttt tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc
    13681 caggaattgc atttggccca gaaggagaag gttatatccg gatttcttat gctagcagca
    13741 tggaaaaatt acatgaggcc tgcaaacgga tcaaagactt tttaacagaa cagtgattca
    13801 tagattttat caaaaaaaca atcgttaatt ttgcggtata ttctgcaagg agacgattgt
    13861 tttttaaatt tagttcaaat gaacgcgttt cttttgattc ataactaatg taatgttaag
    13921 tttaaattgt ttagaatgat tttaaaagaa tattcaagga ggtttcatca atggaaaaaa
    13981 tcaaagttgt cattgtaggt gcatctcatg gagggcatca atcgatcttg gaactattga
    14041 cacgatacgg tgaaaatgta gatatcacgc tatttgaagc aggagactat atttccttca
    14101 tgtcatgtgg tatggagctg tatttagaag acaaggtaac agatgtaaat gatgtacgta
    14161 atttcagacc agaaaacttt ccgcaatcca atgttcatat tttaaataat cacgaagtaa
    14221 aaacgatcga tgccgataaa aaaacagtaa cggttactag cgcatcagat aacgaaacaa
    14281 aagaatatcc ttatgacaaa ttgattttga gttctggtgt caaaccaaac aatctgccag
    14341 taccaggagc agatctagaa aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa
    14401 tcaaagaaaa attaaatgat ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca
    14461 ttgggatcga agcagcagaa gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg
    14521 tgatcgatag acctttaggc acttatttag atgctgagat gacagatatt ttagaacaac
    14581 acctaaatga aaacggtatt gaagttttga caaatgcaaa aatcgaagct tttactggta
    14641 acggaaaagt tgaggcaatc aaaacaagtg aaaaagaaat cccagcagat ctagtcattc
    14701 aagctgccgg tgtgaaggca aatacagaat ggttaaaagg gatcgtcgac ttagatgaac
    14761 gtggctggat ccaaacaaat gaatacttgc aaacgaatct tccagatgtg tatgcagtag
    14821 gagatgctac acttgcttat tctatcccag cacgcaaaaa aatgccgatc gctttagcga
    14881 ctgttgcacg tagagaagca cgttatgtag taaaacatct ttttgaagaa gtaccaaaca
    14941 aaccgttcgg cggagtcgta ggttcctctg cattaagtgt ctttgactat cattttgcag
    15001 caagcggatt gaatagcttt acagcagcaa aagcaggagt agatgtacga gtgtcttatt
    15061 atgaagatac gttacgacca aaatatgtac cagaagaaca aggaaatcca aaagtgtcca
    15121 tccaattatt ctcggatcca atgacccatc aattgctagg aggagcagta ttatctacct
    15181 atgatgttac aggtcaagga aatgtattgg cactagcgat ccaaaacaaa ctgactttag
    15241 aagaactagc agaagctgac ttcttcttcc agccaggctt tgaccgacaa tggagcatgt
    15301 tgaatctagc cgctcaacaa gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa
    15361 tatttgaaaa gaggttgtga aaaagctgcc ttgcatcaag taataagaac agccaagtaa
    15421 aaacagcttc ttatgttttt aacttggctg gacttattac cgcagatgca agcttttgaa
    15481 caccgtttat tttcaaaaaa gggactgtga cacgatttgt gtaccgaccc ccaaaagtta
    15541 gaccaaaaaa tctaactttt ggaggtcggt attttttatg gttaaatata cttttgaact
    15601 taaattaaag atcgttcatg attatttgga tggcaaaggc gattggaccg taaaaatcca
    15661 gatcaagaga ttgaagcgga aatgcttaaa
//
LOCUS       DACEPD010000063        15356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.066, whole genome shotgun sequence.
ACCESSION   DACEPD010000063 DACEPD010000000
VERSION     DACEPD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.066"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            332..946
                     /gene="gmk"
                     /locus_tag="IW797_001946"
     CDS             332..946
                     /gene="gmk"
                     /locus_tag="IW797_001946"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ1224724.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            951..1256
                     /locus_tag="IW797_001947"
     CDS             951..1256
                     /locus_tag="IW797_001947"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ1224725.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1509..3917
                     /gene="priA"
                     /locus_tag="IW797_001948"
     CDS             1509..3917
                     /gene="priA"
                     /locus_tag="IW797_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ1224726.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3932..4423
                     /gene="def"
                     /locus_tag="IW797_001949"
     CDS             3932..4423
                     /gene="def"
                     /locus_tag="IW797_001949"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ1224727.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4416..5354
                     /locus_tag="IW797_001950"
     CDS             4416..5354
                     /locus_tag="IW797_001950"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ1224728.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5354..6712
                     /gene="rsmB"
                     /locus_tag="IW797_001951"
     CDS             5354..6712
                     /gene="rsmB"
                     /locus_tag="IW797_001951"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ1224729.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6725..7465
                     /locus_tag="IW797_001952"
     CDS             6725..7465
                     /locus_tag="IW797_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ1224730.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7462..9531
                     /gene="pknB"
                     /locus_tag="IW797_001953"
     CDS             7462..9531
                     /gene="pknB"
                     /locus_tag="IW797_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ1224731.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9696..10595
                     /gene="rsgA"
                     /locus_tag="IW797_001954"
     CDS             9696..10595
                     /gene="rsgA"
                     /locus_tag="IW797_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ1224732.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10608..11258
                     /locus_tag="IW797_001955"
     CDS             10608..11258
                     /locus_tag="IW797_001955"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1224733.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11259..11897
                     /locus_tag="IW797_001956"
     CDS             11259..11897
                     /locus_tag="IW797_001956"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ1224734.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12052..12906
                     /gene="mreC"
                     /locus_tag="IW797_001957"
     CDS             12052..12906
                     /gene="mreC"
                     /locus_tag="IW797_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ1224735.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12910..13419
                     /gene="mreD"
                     /locus_tag="IW797_001958"
     CDS             12910..13419
                     /gene="mreD"
                     /locus_tag="IW797_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ1224736.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13674..15248
                     /locus_tag="IW797_001959"
     CDS             13674..15248
                     /locus_tag="IW797_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224737.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         5149 a         2879 c         3332 g         3996 t
ORIGIN      
        1 tattaagcca aatttatgca aaaatgtgag aaactatttt cacaaaattt ttcttcatac
       61 tcgaaactaa tcggcttctt cacgacctct tatacacggc gttcaaaagc ttgcatctgc
      121 gataataacc ccaaccaaac gaaaaacatg agaagctgtt tatgtttggc tgttcttatt
      181 acttgatgca ggacagcttt tttacgacct cttggtacag ggtagctttt ttaccaattt
      241 ctagctataa ttaaagataa ccttgaaagt tgcagaaaaa aagtgcaaaa taaacgataa
      301 gtacaaaaag actagttgaa aggacgtcgt catgtcagag cgtggattat taatcgtgtt
      361 gtctggtcca tccggtgtag gaaaaggcac agtacgaaaa gcaatttttg agagtgagga
      421 taatgatttt cagtattcga tctcaatgac tacccgtaaa atgcgtgaag gggaggtcga
      481 aggcgttgat tatttcttcc gaagcaaaga agagtttgaa tcaatgatcg aagctggaga
      541 gatgctggaa tacgcagaat atgtcgggaa ttattatgga actcctttgt catatatcaa
      601 caagacattg gatgaaggaa aagatgtttt cttggagatt gaagtccaag gagcaaaaca
      661 agtcaaagaa aaagtgcccg atggtgtatt catctttttg actccgccag atttagcgga
      721 attaaaatca cggatcgtag gccgaggaac agatgcagat gaagtaatcg aagaacgaat
      781 gaaagttgca aaagaagaaa tcgaaatgat ggctctttac gactatgctg ttgtaaatga
      841 tgaagtgccg ctagcagttg aacgaatcaa aaacattatt gccagtgaac attttcgagt
      901 agatcgcgta atcggtaaat atattaaaat gttaaaggag atgtagtctt atgatgttaa
      961 aaccttcaat tgactcacta ttagatcgtg taaattcaaa atattctcta gtgattttag
     1021 caagtaaacg cgcgcatgaa ttggatgcaa gtgcacagcc tactctagat tcttttgatt
     1081 cagtaaaaag cgtcggtcaa gctttagaag aaatcgatgc aggaaatgta gtcaatgatc
     1141 cacatcctga attaaaacgt gaacgtttga agatggaaga agaggaacgt caagcacaaa
     1201 aagagcgtga acaacacgaa ttggaatcac gtatccgtga agagcaaaaa atgtaataaa
     1261 gaaaaataga gcgaacatct aaaaagaaaa gcaaacccag ctgtttcctt ttcaaacatt
     1321 cgccacttat tgtctggtag aaaaggctgt gtctgaactt tttgacacag ttcttttttt
     1381 tcttatctgc tgttaatttt ttgttaggat ttggcagaag ataagacaaa tccatcgatc
     1441 tctagtaaaa taggcaagga actttttgtc gcttgataag aaaagacaaa aaaaggagga
     1501 aacgaaccat gacattaaca gctgaagtca ttgtggacgt acctactatg cagaccgatc
     1561 agccattcac ttatttggtt ccctctgaaa tggaaacagc tttgcaagtc gggatgcgag
     1621 tagaagtccc ttttggtaat ggcaatcgtc atgtccaagg atttgttatg gctatccaaa
     1681 aatcagaaga atcagctaat ccatctttga aggcagttat tcgtttgcta gaccttgctc
     1741 cggtggtcaa tgaagaatta ctttcattag ctgattatat gaaaaaaatt acttacgcat
     1801 tcaaaatcac ttgtctacaa acgatgcttc cgagtgtgat gaaagcagaa tatgataaat
     1861 tgatctatcc tttggcagac acaccagaag ttcaggtatt gtttgatcag cgagaagtga
     1921 tttcctggaa ggaagcagaa gaagaaggca gcctttctca attgattcgc tggcgccaag
     1981 agcaattagt agatatcaaa tatgaagtcc atacacgaaa taaagtaaaa acgattcgtt
     2041 tggtccgttc tttgttaact gaaaagcaaa tcgaagaaga atgggcaaaa ttacggcaaa
     2101 atgccaagaa acaaaaagaa cttcttttgt gtttatctga actgagccaa gaagaaccaa
     2161 ttgcttattt caaaaataaa ggaatcagta ctgctgtctt gaatcaagga aaagaaaagg
     2221 gctggctgga gtttatagaa tcagaacgtt atcgcgaccc ttataaagac cgagtatttg
     2281 accaaacgac ggcattggaa ctgaatgcag aacaaaaaaa tgctgtcgaa caaatcatca
     2341 cagcaggtca gcagcaaaaa gatcaggtct ttttgctgga gggaatcact ggcagcggga
     2401 aaacagaagt atatcttcag gcaatcgctg atgttttgtc agaaaataaa acggctatta
     2461 tgttagtgcc tgaaatcgca ttgactccgc aaatggtcga gcgattcaaa ggacgcttcg
     2521 gagaatcagt tgcggtctta catagcggac tttcccaagg tgaaaaatat gatgaatggc
     2581 gcaagattga gcgggaagaa gcgcaagtag tagtaggcgc acgatctgct atttttgccc
     2641 cgctgaaaaa tatcgggctg atcattatcg atgaagaaca tgaatcaagt tacaaacaag
     2701 atgagacacc gcgttatcat gcgagagatc tagcaatttg gcgcagtaaa tatcatcatt
     2761 gcccaatagt tctaggaagc gcgacgccct ctttagaatc aagagcacga gctcaaaaag
     2821 gcgtttatca gttattgcag ttaaaccacc gagccaaagc agctgcacag cttccagcta
     2881 ttgagatcgt agatatgcgt gaagagtttc agaatcatcg gacctctact ttttctgcta
     2941 atttgcagga aaaaatacaa aatcgattag acaaaaaaga acaaactgtc ctactgctca
     3001 atcgtcgagg atattcgtct tttgtgatgt gtagggactg tggatttgtt cttccatgtc
     3061 ctaattgcga tatctctctt acactgcata tggatacacg ttcaatgcgc tgccattatt
     3121 gcggacatga agaacctatt cccaatcgct gtcctaattg tggcggcaat aaaatccgct
     3181 actatggaac aggtactcaa aaagtagaag aggaactccg agaactttat ccgcaagcta
     3241 ggattttgcg tatggatgta gataccactc gaagaaaagg ggcacacgaa cagattttac
     3301 aaaaattcgg tgcaaaagaa gcagatatat tattagggac acaaatgatt gctaaaggtt
     3361 tggactttcc agaagtgaca ttagttgggg tgttgaatgc tgatacctcc ctgaatttac
     3421 cggattttcg ttctagtgag catacattcc aattgcttac ccaagtttca ggacgagcag
     3481 ggcgagcgga aaaagcaggc gaagtcgtga tccaaacatt caatcctcaa cattacgcca
     3541 tagaattagc taaaaaacaa aattatgagc agttttatca gcaggaaatg catgtacggc
     3601 atcgtggagg ctatcctcct tattacttta ctgtgaaaat aaccgccagc catcctgaag
     3661 aacaagtagc ggcaaagaaa atctttcaga ttgccaatca gctaaaagaa gtgttaagcc
     3721 ctcaaagctt gctattaggg ccaacgccca gcatgatttt gagagtgaaa aaccgatatt
     3781 attatcaact aatcatcaaa tataaacatg aaccgaatct tcctcatgtt ttggatgaaa
     3841 ttttgaatgg aagccagaaa gaacagcgac aaggtctttt tgtggcaatt gacaacgaac
     3901 cattaaactt tatttaagaa agggccaaat tatgcgctat cctatattga tccaccctaa
     3961 tgataaatta aaaagaactg ctcagccaat cgatgtaatc acggacgaga caatcgcttt
     4021 attagacaat ttatatgaga caatgattgc gaatgacgga atagggatcg ctgctcctca
     4081 agtaggacaa aacaagagaa ttgctgtgat cgaagtagat gaaggcgaga aatttgaact
     4141 gattaaccca gaaatcatcg aagcaaaagg tgaaagcctt gacgtagaag gttgtctcag
     4201 tattccgcat gtctatggaa cggtcaagcg agcagatgaa gtgaccgtgc gttattatga
     4261 tcgagacgga gaagagatag aagtgacggc ttttggctat ttagcccgtg ccttccagca
     4321 tgaaatcgat cacttagacg gtatcttgtt cattgagaaa atgatacagc agatcccgga
     4381 agaagaactt gaagaatata tggaggaaca tcttgatgac taagctagta tttatgggca
     4441 cacctgcttt ttctgtgccg attcttgaag ggctgataga atcaggttac gagatccagg
     4501 cagtggtaac acagcccgac cgtccagttg ggcggaaacg agtaattacg ccgacaccgg
     4561 taaaagaagc ggctctgaag cacggtatcc gtgtgctgca gccagagaaa atctccggtt
     4621 cgccagaaat ggaagaaatc attgaattgg tgcctgatgt gatcgtcact gcggcatttg
     4681 gccaattctt gcctgaaaag ttattgcaag taccaaaatt aggggcaatc aatgtccatg
     4741 cttctttact tcctaaatat cggggaggtg cgcctgttca ttatgccatc atgaatgggg
     4801 agaaagaaac tggtgtaacg atcatggaaa tgatcaaaaa aatggatgca ggcggaatct
     4861 atgcgcaaga aagcatacca atcactaaac aagatgatgt agggacgatg tttgaaaagc
     4921 ttagcctttt aggaagaaag ctattgctag aaacattgcc caatattttg gatggacaaa
     4981 aaccagtacc tcaagacgag tcagaagcaa ctttttcgcc aaacatcaca cgggaacaag
     5041 aagcaatcga ttggcataaa actgcagaag aaattgacaa ccaagtacgc ggaatgcgtc
     5101 cttggccaat tgcattcacg acctatgagg gcatacgctg gaaattatta tcggttgaag
     5161 tattggcaga aactacagac aaagcacctg gtacgatcat ccggaaagac aaaaaaaatc
     5221 tatggattgc ttgcggtaaa cagaccgtct tagccatcaa tgaattacag ccagctggta
     5281 aaggaaaaca ggccgtacat gatttcctta acggaagcgg tcagcaagtt cagattggtc
     5341 agcaggtgaa ataatgaaaa aaatccctaa aaaaatgaaa aaatcagtac gatatatggc
     5401 attactgaca ttagagcgag tgaacaaggg tggcgcctac tcgaatcttt tgttgaatga
     5461 gatgatcaaa caaagcgagc taaatcaaaa agatgtgggt ttatttacag aattgatcta
     5521 tggtacgatt agccatcagc gactattgga attttacgtt gcaccattga tcgctaaagc
     5581 aaaaaaagtg gatgactggg tcaagacgct tctttatctt tctgtatacc aattagaatt
     5641 tttagataaa gtccctgcac atgctatttt aaacgaggcg gtcgaaatcg caaaagtaaa
     5701 aggaaatcct gggacaggga aatttgtaaa tggtgtcttg aggaattatc agcgtcaagg
     5761 agcaccagat ctgcaaggca ttgctgatcc tatcgaaaga ctttctgtca aaatcagcct
     5821 gcctctatgg atgacgaaac gactggtgga tcaaatcgga tacgaagaaa ctgaaaagct
     5881 cggtttatcg ctatatcagc ctagccatgc tagtgcccga gtagatacac gccgactttc
     5941 cagagatcag gccatcactg ttttgcaaga agaagagatc acagctgaaa aaagccaagt
     6001 cagtccatac ggtatagtag cgaaaaaagg gcatttagct tctagctcat tgttccacga
     6061 tggcgtcatg acgatccaag atgaaagttc gatgcttgta gcgccgtcta tgcaaatcga
     6121 gcctgaacat catgtcttag atgcctgtgc agcacccgga ggtaagacga cgcatattgc
     6181 ttctttttta gatccgtcat gcggtggaga agtggtcgct ttagatgtac acgaacacaa
     6241 agtcaaactc attgaagata atgcagcacg tctaggtgtc tcttctgctg tctacgctca
     6301 aaagatggat gctagagaag tcagtaaaca gttcgaagca gagcagtttg atcggatttt
     6361 agtagacgcg ccttgttcag gtcttgggct gcttcggcga aaaccagata ttcgttataa
     6421 aaaacagccg gatgaattag cgaatttacc aaaaatccaa ttggaaattt tagaaagctg
     6481 cgcaccaact ttaaagtctt ccggtattct gacttatagc acttgtacga tcttaaaaga
     6541 agaaaaccaa gacgttgttt ctgctttctt ggaaagacac cctgattttg agaggattga
     6601 tgttttgacc aatcctatgc tacaatcatc aatcgaagag aaaatgttga ctttgtatcc
     6661 gcatcagttt tacacagatg gatttttcat ttgttgtttg cgcaaaaagt aatgaggtga
     6721 aaaaatgcag attgaatatc aatcagatgt aggaagacga cgtaacacaa atcaagacta
     6781 tgccagtgta tttaccagcc gttcaggtct caagcttgct gtattagcag acgggatggg
     6841 tggtcatcgt gccggagaca ttgccagtca gatggcggta acgaatctag gcgaagcttg
     6901 ggaaaaacaa gaagctgttg acgatgaaaa aatcgcccaa tggttcattc aatcgattca
     6961 agaagaaaat atgctgattt atcagcgtgg acaagaacag ccggaatata acggtatggg
     7021 aacaacaatc gtagcggccg ctctgtcaga tgactggttc acgattgcca atgttgggga
     7081 tagcagagct tatttgattc gtgaagataa aatcatgcaa ttgacggagg atcattcttt
     7141 agtcaatgaa ctagtcaaat caggagaaat cagcgaagaa atggcagtga accatccaag
     7201 gaaaaatatt ttaacacgtt ctgtcggcat gcctggaaca gtcgaagtag atgtttctac
     7261 tcatctttgg caactaaaag atcgactgct gttatgttca gatggcttga cgaacatgct
     7321 gtctgaagaa gaaataggaa cgatcatcaa tcaagaaggc ccattgtctc ataaagtaac
     7381 tgagctgatt gatcgtgcca atgaagcagg cggagcggat aatatcaccg ttcttcttat
     7441 cgaatataag gaggacagtg tatgatcgag ttgggcaaaa aattaaatgg ccgttatcaa
     7501 atcactggaa atatcggtag cggtggtatg gcaaatgtat ttttggcgca cgatttaatc
     7561 ttggatcgtg atgtagcagt taaagtctta cgatttgact ttcaaaatga ccagactgcg
     7621 attcgacgat ttcagagaga agctttagca gcaacagaac tcgttcatcc aaacattgtt
     7681 agtgtttacg atgttggtga agaagacaac atgcagtatc tggtgatgga atacgtcaaa
     7741 ggaatggact tgaaacgata tatccaaaca cattatcctg ttccttatga aacagcagta
     7801 aatattatgc agcagatcct ttctgctatt tctttagctc ataaccatca aatcatccac
     7861 cgagatttga aaccccaaaa cgtactgatc gataatgaag gtgttgtaaa aatcacggat
     7921 ttcgggattg ctatcgcttt atctgaaaca tcgatcacgc agacgaatac gatgctggga
     7981 tctgttcatt atctgtcccc tgagcaggcc agaggaagta tggcaacgaa gcagtcagat
     8041 atttatgctt tagggatcat cctttatgag atgttgacgg gtagcgtacc gtttgatggt
     8101 gaatctgctg taacaattgc tttaaaacat tttcaagatg atcttccatc tataaaagca
     8161 cttgatccaa atgtgccaca agcactggaa aacgtgattt tacgggcaac ggctaaagaa
     8221 cctgcagacc gttataagtc agccgaggaa atgtctgatg atctttctac agtattatca
     8281 ccagctcgtg caaacgaaga aaaatggcag cctcatgtga tggataatga aacaaaagtt
     8341 atcacacccc ttacggaaga tgcaccaatg cctgattcat tcaaatctat gcctctgcca
     8401 aaagacaagc cagaaaacga aaccttgcca gaggaggaag aagttctgcc tgatcctaaa
     8461 aagaaaggac agaaaaaatg gtggctgatc ctattagcta ttcttgctgt tttaggaatc
     8521 ggaacagctg tattctttgc ttctggcggg cgaggagaag ttgttgttcc tgatgtttct
     8581 gatctttctg aagcttctgc tagagaaaca ctcgcaaaag cgaaattaga agtagcaaca
     8641 aaaacagaag aaattgcaga tgacaaaatc gaagaaggta atgtagtgaa gacagacccg
     8701 gcagcgggaa cgacagtcaa gcaaaaacgc gaagtcactc tgtatattag tagcggcacg
     8761 aaaaaaatca aattagatga ttacagtggt aaaagttatg aagatgccga agcaagtttg
     8821 aaagaattag ggttttctgc tgacttgatt caaaaagaag aggaattcag ttcagaggta
     8881 gaaagcggaa tgatcatttc gcaatctcca tcagaagata cagaagtcga tcctaaaaca
     8941 gacacgatta ctttcacagt cagcaaagga ccagaaacag tgactttgag tagtcatgta
     9001 ggagaaaact acgaaactgt tcgtaattgg cttgttacac aaggattcaa tatcaataat
     9061 attagtgcta cttatgacta tagcgacact gtaccatcag gttcgatcat cagtcaggat
     9121 ccagcctcgg gtgaagttgt agcagaagaa acttacatca attttgtcgt aagccaagga
     9181 acagagccta aattgaatga tatctccggc tatacaaaaa gcgaagcaca aagttattta
     9241 gctagtgtcg gtgcagaata tatcgggcat gagacttccg aatattcaaa cacggtggat
     9301 aaagataaag tgattcgtac agtaccaggg gcaggaacga caattaccaa aggaatggtt
     9361 gtgaacgtaa tttactctaa aggaccagat ccagcgtctt ctagttctga aaaagactct
     9421 tcagacagtt ctagcagctc aagtagctcc agtagcacaa gcaactcttc aaaagaagaa
     9481 tcttccagct ccacaagttc atcagctagc acctcagaaa gttcaaaata agtgctgaat
     9541 aaaatatatc agaagggaat gcgacattga tttgttgcat tctctttttt gtgtttctat
     9601 ctcttttgat caattttcaa gccatatact ttgagaagtt tgcatggaat aacacctact
     9661 ttcctcttcg tatggtaaaa taaacaaaaa cggaggtggc acttatcaaa ggacaaatca
     9721 gaaaagcaat cagcggtttt tactatgtat acgcaaacgg agagacctat caaacaagag
     9781 gaagaggaaa tttccggaat cggaaaatca cgccgcttgt cggagatcag gtgatctttg
     9841 agagtgaaaa tcaaacagac ggctatcttc tggaaatctt gccgagaaaa aatcaattag
     9901 ttcgcccacc agtcacaaat gttgatcttg gaatcattgt cacaagtatc gtagagcctg
     9961 cattttctta taatctactt gaccgctttc tagttacctt ggaatacgag tcgatcgaac
    10021 cgattatttt tttgacaaag accgacttgt cagacgaccc agaaaaaatc gaagagatca
    10081 agcaaaccta tactaaaata ggctatccgg tgattgtacc caatcatacg ggtgatacga
    10141 cagaactgat ccgttacttc ccagaacggc tatcggtctt tatgggtcag tctggagcag
    10201 gaaaatcgac gctactgaat caaatatcac cggaactgaa tttagcaaca ggagagattt
    10261 ctgatgcact tggaagagga cggcatacaa cacgtcatgt ggaactgatt cctatctatg
    10321 gcgggttagt agcagataca ccaggattta gctcgattga ctttttgaca attgaagcag
    10381 gagaactgcc gagacaattt cctgaatttg tagaagcttc tgtccattgc aaatttagag
    10441 agtgcatgca tgctaaagaa cctggctgcg aagtaaaaca tagagtagaa acaggtggta
    10501 tcgcacagac acgttacgac aattatttgc aattcttaca agaaatcgaa aaaagaaaac
    10561 caatgtatgc aaaaaaagaa ggtaaacgaa aataggagga aacaagaatg aagatagcac
    10621 catccatttt gagtgcagat tttgccaatt tacaaagaga cattgagtta gtggaaaaag
    10681 gaggcgcaga ttacatccac gtagatgtaa tggatggcca atttgtccca aatatcacat
    10741 tagggccaaa tattgttcag gcaatccgtc ctgttactaa attaccgtta gacgtccatc
    10801 tgatgatcgt tgatccagaa aactacattc cagcatttgc aaaagctgga gcagatatca
    10861 ttactgtcca cgtcgaagca actcctcata ttcatcgggc acttcaaatg acaaaaagct
    10921 taggagttaa agctggagta gttatcaatc caggaacacc agtctccatg atcaaacatg
    10981 ttttgccttt ggctgatcaa gtactagtca tgactgtcaa tcctgggttc ggcggacaaa
    11041 gctttattga agaaacagta gaaaaaatta cggaactcgc tgatctacgt agagagaaca
    11101 attggactta tgcaatcgaa gtagatggcg gaatcgttcc agaaacagca aaaatctgta
    11161 aagatgcagg ggcggatgtc ttcgttgcag gatcatatat ctatggagcc gaaaatccag
    11221 tcggacaaat cgaacgatta aaagaggcat tagactaaat gaaaatttta ttagctgctg
    11281 gaggtaatcc gcaaaactgg ccgcagtctc tgctgtccaa atacgatgcc tatgtcggga
    11341 tagaccgtgg tgcgctgtac ctgcttcaag caggctaccc agtagatctg gcagtgggcg
    11401 attttgattc attgtcgaac gaagagcaga aggaagtatt tgcaaaagcc aaagagtata
    11461 cccgttcccc tgcagaaaaa gacgatacag atacacagtt aggtatctta aaaacatttg
    11521 aacgttaccc gcaagcggaa gtcactttga ttggagctac gggcggaaga ttggatcatc
    11581 ttttagccaa tctctggtta ggtatggaac ctcgcttcaa accctttgcc catcagctta
    11641 ttatcagaga ttgtcaaaat tatttgtctt attatttgcc tggagagcat atagtaagac
    11701 agataccaga tatgaagtat ttagcttatt gctgcttgac tcccgtctcg aatcttacat
    11761 taaaaggcag caagtatgta ttggaaaata aagaagtaga atatccgaca tcttatgcaa
    11821 gtaacgaatt tctttcagat accgcagaat tttctttttc agatggtatg attgcagtta
    11881 ttcaaagcaa agattgatcc ataaacaaat aaaaaaattt gtctttcagg ctaaaaatga
    11941 cagaatctat tatttgctaa aaaaaagctt aagaggagca acttaccttt tgtttcacgg
    12001 catttagtga taaaattgaa cacaagagtc taattaattc gaggtgtctt tgtgaagaag
    12061 ttcaatccta ataaaaatat tattattacc ttggttattg ttatcatcgt ggtgacgata
    12121 ctaagtgtga ccgctgcgca gcgagcgaat gaaggaaagc ccaatttctt ccaatccatc
    12181 gttaatgaca gtgttgcaat ggttgataaa acaatctctg ccccagtacg gtgggtacga
    12241 aatggagtag attctgtcca taatcttttt tcaacttatg ccgaaaatga gcggctaaaa
    12301 gaaaaaatcg atagctatga tgacgtttca cagcaaaaca agaattacga gagagaaatc
    12361 gaagcgttga aaaaagaact tgagttgaat cagacgctga ctagttacga aaaagtaacg
    12421 gcaaatgtca ttactcgttc gcctgacagt tggcaggaca tgctgatcgt agacaagggt
    12481 tctaaagacg gtatcgaagc aaatatggca gtcatgtcgc aaaaaggatt gattggccga
    12541 gtgatcgagg tcaatacggc ttcgtctaaa atcgaattac tgtcatcctc taatgaaagc
    12601 tccaatcatt ttccagtacg ggtatcttcg gctaatggcg aagcgtttgg tttgcttaaa
    12661 aactatgatg aaaagctcca tgccttagtg gtgacccaat taactggtga tacggatatc
    12721 aaagaagggg atgttgtcca gacatccggt cttggaggga attctccagc taacttgccg
    12781 atcggtacgg ttattaaaac gaaaccagat agttatgggc tggatcggga agtttatgtg
    12841 aaaccttatg cagaaatgta tgacgtgtca gttgtgacga ttgtccaacg gctagcggag
    12901 gattagtcca tgttgaagaa agaaacaatg aagtactatc tgccaatcgt tttgttcttt
    12961 ttgatgttga tagatggtca tttaacaaga atgctagggg agtggtcgaa aggcacctat
    13021 atgtcaaatg cccactttct gatattggca ttattatgtt gcagtatggc gtttgaaaaa
    13081 cgttatttac tgattaccac gattgttctc ggggctatct atgacgctta ctatattggc
    13141 gttatcggta tctatgcagt agctctccct ttaattgtat ggttgatgta tgtaatgaaa
    13201 gacgttatcc atgtcaacat ctttactgaa tttttcagta tgatcatctt tgtcacgggt
    13261 tatgaattgt ttacgatggt ggtccagttg atttttaaat tagcagtagt aaataacacg
    13321 tattttatta caaggttttt aggacctaca ctgctgttga acatgattat atttgtatta
    13381 ttcatttttc cctttaagaa attattcagt gatgaataat ttcttttttt atttttaggt
    13441 cgaaagcgaa aaagaatcgt tgttatctta agaaatgcta gacttaacgc ggctttttca
    13501 tgatcatttt ctgaaacgaa cagttttgtc atttttaaaa gttgttttga aacatttttg
    13561 taacataact attatatcgt tgacacactg ctatggtaca atttttatat cgttaagaaa
    13621 gacttaaata tatttagata tttattaaat taagccagtc ggaggaatga agagtgaaaa
    13681 agagtttaat atcagcagta atggtatgtt caatgacctt gactgccgta gcatcaccga
    13741 tcgctgccgc ggcggacgat tttgattctc agatacaaca gcaagatcaa aagattgcag
    13801 acttgaaaaa tcaacaagca gatgcacaat cacaaatcga tgccttagaa agccaagttt
    13861 ctgaaatcaa cacacaagca caagacctat tagcaaaaca agatacattg cgtcaagaat
    13921 ctgcacaatt agtaaaagac attgcagatt tacaagaacg tattgaaaaa cgtgaagata
    13981 caatccaaaa acaagcacgc gaagcacaag taagcaacac aagctcaaac tacatcgatg
    14041 ctgtattgaa tgctgattca ttagcagatg ctattggacg cgttcaagca atgacaacga
    14101 tggtaaaagc aaacaacgac ttgatggaac aacaaaaaca agataaaaaa gctgtagaag
    14161 acaaaaaagc tgaaaacgat gctaaactaa aagaattagc tgaaaaccaa gctgctttgg
    14221 aatctcaaaa aggcgatctt ttatctaaac aagctgattt gaacgtactg aaaacttcat
    14281 tagctgctga acaagcaact gctgaagaca agaaagctga cttgaaccgt caaaaagcag
    14341 aagctgaagc agagcaagct cgtattcgtg aacaacaacg tctagcagaa caagctcgtc
    14401 aacaagctgc acaagaaaaa gctgaaaaag aagcacgcga acaagcagag gctgaagcac
    14461 aagctactca agcttcatct actgcacaaa gctcagcaac agaagaatca tcagctacac
    14521 aatcttcaat gactgaagaa tcatcatcag ctacacaatc ttcagcaaca gaagaatcaa
    14581 caacacctga atcttcaaca gaagaatcaa cagcgcctga atcttcagca acagaagaat
    14641 caacaacagc gcctgaatct tcagcaacag aagaatcaac aacagtgcct gaatcttcag
    14701 caacagaaga atcaacaaca gtgcctgaat cttcaacaac agaagaatca acaacacctg
    14761 cgcctacaac accatcaaca gatcaaagtg ttgatacagg aaacggcaca ggaagtagta
    14821 ctccggctcc aacgccaaca ccaacacctg aacaaccaaa acctgtaaca cctgctccag
    14881 ctccttctgg aagtgtgaat ggtgcagcaa tcgtagcaga agcttacaaa tatattggta
    14941 ctccatatgt ttggggcggt aaagatccaa gtggctttga ctgctcaggg ttcacacgct
    15001 atgtttacat gcaagtaact ggtcgtgaca tcggtggttg gacagttcct caagaatcag
    15061 ctggaacaaa aatttcagtt tctcaagcaa aagctggaga cttgttattc tgggggtcac
    15121 aaggcggaac ttaccacgta gcgattgctt taggcggagg acaatatatc catgctcctc
    15181 aaccaggcga aagcgtaaaa gttggttcag ttcaatggtt tgcccctgac tttgctgtca
    15241 gcatgtaaga atgattaatc aaaaagagtt agcccattct ggactaactc tttttttatt
    15301 gtgataaaat agaaatcaga taaattcttc tgtcaattcc atgaacgttt ggatat
//
LOCUS       DACEPD010000064        15313 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.067, whole genome shotgun sequence.
ACCESSION   DACEPD010000064 DACEPD010000000
VERSION     DACEPD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.067"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            278..787
                     /locus_tag="IW797_001960"
     CDS             278..787
                     /locus_tag="IW797_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1224738.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IW797_001961"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IW797_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1224739.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IW797_001962"
     CDS             2322..3302
                     /locus_tag="IW797_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1224740.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IW797_001963"
     CDS             3424..4842
                     /locus_tag="IW797_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ1224741.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IW797_001964"
     CDS             4913..6382
                     /locus_tag="IW797_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1224742.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IW797_001965"
     CDS             6392..6859
                     /locus_tag="IW797_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1224743.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IW797_001966"
     CDS             6895..7767
                     /locus_tag="IW797_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1224744.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7860..10382
                     /locus_tag="IW797_001967"
     CDS             7860..10382
                     /locus_tag="IW797_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1224745.1"
                     /translation="MKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQLFSD
                     GKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLPLIIK
                     GNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKKGAFT
                     GAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEYANVR
                     LILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITIDSKIF
                     CSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWIDPEI
                     SIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESDEFIA
                     LRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFRMKND
                     KQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITASAIAR
                     ETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKI
                     ESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKN
                     VIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTICVLST
                     TEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTKLRFL
                     DSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHLKEGA
                     QLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="IW797_001968"
     CDS             10538..11311
                     /locus_tag="IW797_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1224746.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="IW797_001969"
     CDS             11304..12095
                     /locus_tag="IW797_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ1224747.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="IW797_001970"
     CDS             12105..12596
                     /locus_tag="IW797_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1224748.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="IW797_001971"
     CDS             12614..13024
                     /locus_tag="IW797_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1224749.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="IW797_001972"
     CDS             13028..14428
                     /locus_tag="IW797_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ1224750.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="IW797_001973"
     CDS             14451..14825
                     /locus_tag="IW797_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1224751.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5314 a         2156 c         3060 g         4783 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacaa ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacctacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag agtaagcgcc
    15241 ccatagaaca gtaccttact aacaaatgca cctcaagata tacttgagct atcaagaagt
    15301 ctggaggttt tct
//
LOCUS       DACEPD010000065        15311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.068, whole genome shotgun sequence.
ACCESSION   DACEPD010000065 DACEPD010000000
VERSION     DACEPD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.068"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(41..1336)
                     /gene="purB"
                     /locus_tag="IW797_001974"
     CDS             complement(41..1336)
                     /gene="purB"
                     /locus_tag="IW797_001974"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ1224752.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1349..2482)
                     /gene="purK"
                     /locus_tag="IW797_001975"
     CDS             complement(1349..2482)
                     /gene="purK"
                     /locus_tag="IW797_001975"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ1224753.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2466..2954)
                     /gene="purE"
                     /locus_tag="IW797_001976"
     CDS             complement(2466..2954)
                     /gene="purE"
                     /locus_tag="IW797_001976"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ1224754.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3370..4677)
                     /locus_tag="IW797_001977"
     CDS             complement(3370..4677)
                     /locus_tag="IW797_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ1224755.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4680..5261)
                     /locus_tag="IW797_001978"
     CDS             complement(4680..5261)
                     /locus_tag="IW797_001978"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ1224756.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5377..5474)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5833..7125)
                     /locus_tag="IW797_001979"
     CDS             complement(5833..7125)
                     /locus_tag="IW797_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1224757.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7122..7745)
                     /locus_tag="IW797_001980"
     CDS             complement(7122..7745)
                     /locus_tag="IW797_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224758.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7748..8584)
                     /locus_tag="IW797_001981"
     CDS             complement(7748..8584)
                     /locus_tag="IW797_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1224759.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8601..9488)
                     /locus_tag="IW797_001982"
     CDS             complement(8601..9488)
                     /locus_tag="IW797_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1224760.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9504..10787)
                     /locus_tag="IW797_001983"
     CDS             complement(9504..10787)
                     /locus_tag="IW797_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1224761.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11470..12153)
                     /locus_tag="IW797_001984"
     CDS             complement(11470..12153)
                     /locus_tag="IW797_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1224762.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12171..12788)
                     /locus_tag="IW797_001985"
     CDS             complement(12171..12788)
                     /locus_tag="IW797_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ1224763.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12798..13622)
                     /locus_tag="IW797_001986"
     CDS             complement(12798..13622)
                     /locus_tag="IW797_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ1224764.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13615..14175)
                     /locus_tag="IW797_001987"
     CDS             complement(13615..14175)
                     /locus_tag="IW797_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224765.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14349..15305
                     /locus_tag="IW797_001988"
     CDS             14349..15305
                     /locus_tag="IW797_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1224766.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
BASE COUNT         4496 a         3416 c         2621 g         4778 t
ORIGIN      
        1 tgctattcta ttttaattga attttgcaac tcactcttct ttattccaaa cctactcgtt
       61 tgaatatcac gtcaacgttt ttcaaatgat gatgataatc aaaggcatcg tctatttctt
      121 ctttagataa taccgatgtg atggttggat cttcttctaa cagcgggcgg aactgcactt
      181 ggttgtccca agcgtacgca gtttttggct gaacaagatc ataagcttct tctcttgtca
      241 ttcccttatc gatcaatttc agcataacgc gctggctata aatcaaacca aatgttgcag
      301 acatattccg cttcatgttt tcaggaaaga ccgttagatt tttaacgata ttgccaaaac
      361 gatttaacat gtaattcaat aaaatcgtgg tatcaggcaa gatgattcgt tctgctgaag
      421 aatgagaaat atctctttca tgccataatg tgacattttc ataggcagtg accatgtgtc
      481 cgcgcaccac tctggctaga ccagccatat tttctgaacc gatcggattc cttttgtgcg
      541 gcatagcaga agaacctttt tgacctttcg caaaaaattc ttctacttca cgtgtttcgg
      601 atttttgcaa tccgcgtatc tctgttgcaa aacgttcaat acttgtagca ataagcgcca
      661 tcgctgagaa atattcagca tgtagatccc gagggagaac ttgagtagaa atttcttgtg
      721 gacggatccc caattgttta catacatact cttcaataaa tggcgggata tttgcgaacg
      781 ttcccactgc tcctgagatt tttcctgctt ctactccttt tgccgcatgt tcaaatcgtt
      841 cgatgttccg tttcatttct gaataccatg tggcaagttt caatccaaaa gtagttggtt
      901 cagcatggac accgtgtgtc cgacccatca tcactgtata tttgtgttct tttgcttttt
      961 cgccaatgat ttccgtaaaa cgtttcaagt cttcccgtaa aatatcattt gcttgcttga
     1021 tgagatagcc ataagcagta tctacgacat ccgtacttgt tagaccataa tggacccatt
     1081 ttctttcttc tcccaatgtt tcagaaaccg ctcgtgtaaa cgcaacgaca tcatggcgtg
     1141 ttaatttttc gatttccaag atccgatcga tatcaaatga tgcatgctca cgaatttttt
     1201 tcacatcttc ttgcgggatt tcaccaagtt ctgcccaagc ttcatctgct aaaatctcga
     1261 cttcgagcca cgcacggtat ttgttctctt ccgtccagat attccccatt tcagggcgtg
     1321 tataacgttc taacatgttt tttctcctct aatcccaaat attcgtttgt ttgatttctt
     1381 ccagcgtttg ctggatgtct tctgtcaaga tagtgatatg tcccatcttg cgaccttttt
     1441 ttgcttgttt ttttccatag aaatggaatt gccagtctgg cttattctca atcaagcgat
     1501 agctttccag cagttgttct cccaaaacat tgaccattac tgcattgctt atcaaacgta
     1561 ctttttctcc taatggccaa ccacaaatac ctcgaatatg ggcatcgaac tgactcatag
     1621 aacaagcttc aatagaataa tgtccagaat tgtgaggacg aggagctaat tcattcacat
     1681 atagcccgcc tgtttttgtt aaaaacattt ctacccctaa aacacctgaa aggctcactg
     1741 cttctgcaat cacacgggca atacgctcag cctcctcgat cacatcctga tcaatcgcgg
     1801 ctggagcaat cgtttcatga agaatattgt tgtggtggat gttttccacg accggaaaag
     1861 tcgtgaaatc tccttgacca tttcctgcca ccatgatcga aatctctttt tcaaacggga
     1921 tccatgcttc taaaacacag gttccttctc ttaaaaggtc catggcagga gctagatccg
     1981 cccgactttt caaaacatac tgccccttgc catcatagcc gcctcgagtg gttttcaaaa
     2041 cacaaggata gccgatcccg tcaatcgcat cttggatatc cgtcggactg acgatcgttg
     2101 cataaggagc aatcacgatg ttattcgttt ccaaaaaaga tttttctaac aggcggtctt
     2161 gcgtaatcgc aagcaagtct gtcccttgag gaataaatga catgggtaaa atcgcattta
     2221 acgcctctat actgacgttt tcgaattcat aggtaatgac atctgttctt ctggcaagct
     2281 ctttcagcgc aaaggtgtca tcatagacac tttcaatgtg ccaatctgca acttgtgcgg
     2341 ctggacaatc gctcacagga tccagcacac ctactttaaa gcccatttct tttgcactaa
     2401 tcgccatcat acgacctaat tgaccgccac cgacgatccc gatcgttgaa ccaggcaaca
     2461 aaggtttaac caagttgatc actactttct ataacagttt tagccaacat tgaacgtctt
     2521 tcagccaatt tagcctccaa ttctaaatca tacatggaaa gcatctgtat cgcaagtagt
     2581 ccagcattaa ttgctcccgc ttttccgatt gctgtagtag ctacaggtac tcctccaggc
     2641 atttggacaa tcgaaagaag cgaatccaaa ccatttaatg ttcgtgattg gaccggtaca
     2701 ccgataaccg gcaaagttgt ttttgatgcg accatacccg gcagatgcgc ggctccccct
     2761 gctcctgcaa taatgatttt gattcctttt tttcgagcgt tttgtgcata ttcaaacatc
     2821 aaatctgggg ttcgatgagc agaaacgacc tttttttcat aagacacacc gaattcatcc
     2881 aatatttgac atgcttcttt cattgtttcc caatctgaag tactccccat aatcacagag
     2941 atttttgctg tcattgtttt tcctcccagt ttttctacat tcatatttta acaatcactt
     3001 tttttgatgt caaagaaatt tcgaatgtta acaaagaaaa acattttatt gttcgtgttt
     3061 attaaagata aaaaaaagga atgtgacaaa agttaggtca cattcctttt tatggataaa
     3121 cggtgttctc ctgccaactc ctcggtcaca aggaacaata ttcaacaaac atgagaagct
     3181 tttttttgaa attgttcctt gtgactcgga gatgaacggc aggctcacga cctcttcctt
     3241 caacggtgtt caatcagctg accctcggta ttagctcaaa caaacggcaa aatacgagga
     3301 actatttttc cgtttgtttc tctaacaatc aggtcaaaac gctgatttca caacctttct
     3361 tttgctctat tacttcttga aggcttcttc caatatttct tcatcttttt tcaatccatt
     3421 aaagatcaaa ttcaatatga tagccgtaat gctactcatt acgatgccat ttccagtgaa
     3481 catttgcaat gtttgcggca tttgactgaa taatgtcggc gtaacattga aacccagccc
     3541 aaatccaaca gaaacagcaa caatcaatag atttttatca ttgctgaaat caacttccaa
     3601 cagcatacgg attccttgga ctgccaccat cccgaacatg actaacattc ctccaccaag
     3661 taccggctca gggatgattt gtgctaacgc accgatttta gggaataatc ctaaaacaat
     3721 caagaagaat gcagagaaat agatgggttt tcttgttttg attcccgaaa gttgaaccaa
     3781 tccgacattt tgagaaaagc cagtataagg gaatgtattg aaaataccgc ctaagataac
     3841 ggcaagtcct tctgcacgat atcccttttt caattcttct tctccaacat ttttcccagt
     3901 aatgtcacca agtgcaaaat atacacctgt tgattctacc atgctgacca tggaaatgat
     3961 gatcattagt aaaatcgacc atacgtcgaa agttggttta ccaaaataaa agggttgtgg
     4021 aacatggaac aacggcgcat ctccaatcgc gctcagatca atcattccca tgaaagcagc
     4081 taagatgctt cctccgacta aaccaatcaa taccgcgatt gaacgaatga acccttttgc
     4141 cagcatttgc acgcctacaa ttaggagaat cgtcacaaat gcaagtagca gatttgtttt
     4201 gtcaccaaaa tcagctgctg ttgcattacc tccacccatt ttctcgattg ctacaggaat
     4261 caaagtcaaa ccaataacgg tgataaccgt tcctgttact ataggaggga atagtttttt
     4321 aattttagaa aagacaccag aaactaacac aacaaagatc ccagaagcga taatagatcc
     4381 gtagatagct cctactcctt cattggttcc gatcaagatc agtggagcta ctgcttggat
     4441 cgcacatcct aaaacgacag gcaagccaat tccgaaaaat cggttaactg tcaattgcaa
     4501 taatgtcgct aatccacaca taaagatatc aattgaaatc aaataagtca tctgttcttg
     4561 attaaagcct agtcctgtac cgatcaaaag aggcaccgct actgctcctg catacatcgc
     4621 tagcagatgc tgcagcccta aaaccgcagc ttttccattt tgtgtttctt tttccacgat
     4681 tatgcgtcct cctcaagaaa ctcaacttgt ccattcgtca aggaagcaat acgcgcaaga
     4741 gaaacgactc tcaatcccat ttcttcaagt aactgacgtc catcttggaa agacttttcg
     4801 atgacgatcc cgattccttc tacttgcgct ccagcttgct ggcataattc gatcaatcct
     4861 tttgccgctt gtccgtttgc caagaaatcg tcgataatca atactttgtc ttctgaagat
     4921 aaaaatttac gagaaataga aatcgtactt gtcacttgtt tcgtaaaaga ataaacagaa
     4981 gctgtcaata attcttcatc catcgtcaaa ctttttgctt ttctagcgaa gatcattgga
     5041 acatctaatt ctcttgcagc aaataaagct ggtgcgatcc cagaagcttc aatggtcaca
     5101 actttcgtga ttccttgttc tttaaatact tctgcaaacc gttttcccat ttgttccatc
     5161 aatacagggt ctacttggtg agtgacgaag ctgtcgactt ttaatacgcc ctcgcctaat
     5221 atacgtccgt ctttttgaat acgttcaact aattctttca ctttatttgc tcctttatca
     5281 tttattaata ccgaaataac cgcttttgtg caaaaaccta ctatgtttct gttaaagaaa
     5341 aacaccctcc actttatcat aagaaaagaa gggtgaaata gccaatcttt tacttatagt
     5401 ccagcattta cggtactggc tagaaactgt catccatatt atgacgatat ataagtaggt
     5461 atgtaaaact aataatagtg taaattcatt ttcttgcaac tgttttgcac gtgaaggcag
     5521 tttgccatat ttgaataaaa aatgttgttt caaatattga aaacagtcta acactacttt
     5581 ctgaaactga caacgtggtt tacttatttt tttgtaaaat ctaatgattt ttaatgcatt
     5641 attccttttt gttcgggttt aaatagttat ttttattttc tcctatctaa aaaagcagcc
     5701 cacataaaaa gaaggcatga caaaaagcat gtcatagccc ctttttccga ataaatagtg
     5761 ttcaaaagac agcctatcgg caataaggca agattttata aactacggga agtaatttcc
     5821 gtaaatctat tcttattctt ttaagctgac aagctcgtca atcactactg cttttcctga
     5881 agcaatagaa tgatttgctg cgattcctgt caaaatcgac aaaataccat ctacgtgagt
     5941 tgctgcccga ttccacggat cttcttcttt ttcttcacca aatagatcat ctaataaacg
     6001 cggatctcct ccgccatgtc cgcccgcttc ttgttcgacg attacctcat atggctcacc
     6061 gaacatcgga tagacatgga tggactgaag aacggctgct ccttcatcct ttttatctcc
     6121 acctgcattg atgtaagact tttcgatcac tttcatttcg atccgtcctt tgctgccatt
     6181 gaaagaaaca ttgaatcctt cccacggcat atacgcgttt agcgaatacg tcaaaatcgc
     6241 accagtttca tatttgaccg ttacgcctag tgtatcttca atagagatat catcggaaaa
     6301 tacactgcgg tcacgaatat atccactgtc tttttctgct tctaaataca tttctcgcaa
     6361 ttcctcattg gctgacaggt ctatagcaaa tggatcagct ttagctagtt cgcttccata
     6421 gcatcgactg taaaatattt ctactccgcg tttctccgca ttatgcatcc cataaaagtt
     6481 taatccaccg aatgcaaata ccttttgggg acggctgttc aaccaaaaat tcactagatc
     6541 aaaatgatgg gtagatttat gaacaagaag cccgccgcta ttgtgcttat ttcgatgcca
     6601 tcttctgaaa taatctgccc cgtgttgcgt atttaacagc cattcgaagt ggatagaaaa
     6661 cacatcacca atcacatcac tttctatcag ttcctttatc ttcgtgtgat acggtgcata
     6721 acgataatta aaagtgactc gcaggcttct tcccgtctgt ttttggaccg ttattatttc
     6781 ttgagctttt tcctcatcga tagtcagagg tttttcgcaa ataacatcat atcctttttt
     6841 cattgcacga atgatataat catgatgcgt tcgatctact gacgtaataa tgacttcagc
     6901 cggccgtacg tcatccagta gttgctcaaa ttgtgatggc atacatgtct ttactttttt
     6961 ctgtccatac ttgttcacaa gtatccgatt tgcataatcc atccttgttt gagatacatc
     7021 acaaaaaccg actagttcac aagtttgatg atacgtagag gcaattgcct cataaaaaaa
     7081 acgcgctctc ccaccaagtc cgacttgtac atattttttc ttcatttaaa gtcattctcc
     7141 ttttctttta agcaaaatac tgtgacaaat ggcagccctg gatacagtaa tgcagtgagt
     7201 aaaaaatcat gctgtataca ccagaacagt ccatacaaaa ggcataaata ccacagaaac
     7261 cccttgacgt tttttgacaa cagccagata ttccttttga tcaaataaac aagactatct
     7321 gctttatctt ctttgcagat atcaattagc gtactaaaaa aactatggga tacaaaaagc
     7381 aacagacaca atagaaaagg aatcagatac caagctccaa tctttcccac taataaatac
     7441 caatcaactg caaaaataat gacaaacagc tgaaacaacg taatgatgaa acacatccgt
     7501 ttgatttttc ctttcctcca acataccttt ttttctgcca agtaatggac agatagataa
     7561 taagaaggat acatcatcaa aaaataaatc agatagacaa cacgagcaga cagcactaat
     7621 cctaaagtga acaaacatcc tagaactaaa gcgaaaagag cattactgat aagactggtt
     7681 aaaaaaatcc gacagacata gtagttgagc ttgtaaagaa acatctcttc tattggcttt
     7741 ttcatattca gcccttcaga ccgcttgtac tgatcccctg gacaagatat ttattgaaga
     7801 ataagaaaat cagaaaaacc ggtagcagcg acaacgtaga catcgcaaac atactcgcgt
     7861 aagtcgtact agatccagcg tctgcaaata atttgatggc aatcgatacc gtgtaagatc
     7921 tcggcttgtt taaataaatc aatggtccca tgaagtcgtc ccatttccaa taaaactgga
     7981 tgatgatcgt cgtgatgatc gaaggtttca gcaaaggaaa aataattttg gtaaagacac
     8041 tatacttgct gcatccgtca ataatcgctg attcatctaa ctctttgggg atgctgacca
     8101 tgaattgcat gatctggtaa atgaaaaatg cttgtccaaa aaaatgtggt aaaatcagcg
     8161 gcacatacgt cccaaccaaa ttcagccgat tgtaaataat aaactgcgga atcaaaatca
     8221 cttgtgcagg aatcatcatc gttactaaca tgacgacgaa ccagaatctt tttcctttga
     8281 aatcgattct cgataatgcg taagcaatac aagtcgagct cagaactgtc cctatcgtcg
     8341 aaactgcaga gatgacaaac gaattcctaa agaaagtaaa aaatgaaatt ccagcaaacc
     8401 ccgcccatcc ttttgcgtaa ttttcccatt gaaaagtctc tggaatcaaa ttcagcgaac
     8461 cactcataat ctcttgatcc gttttaaaag aacctgcgat catccataag accggataaa
     8521 tcatgactag acctaatagc aaaataaaac cgtattgaaa ccatttcgtt actgtattat
     8581 tcatttcttc ctcctctcat tcacacaacg acccattttt ttgatgattt gaaaatcaga
     8641 aaggtgacag ctcccataat caacagcatg atccacgaaa tagcactagc ataccccata
     8701 gaattaaaag aaaaagcttg gttatacaca taaagagcat aaaagttcgt ggcatcattc
     8761 ggaccacctt gggtaatgat aaatgcctgc gtaaaagtca taaacgccgc gattgtctgc
     8821 ataaccaagt tatacaaaat gatcggcgaa agaagaggta acgtgatctt gtaaaacgtc
     8881 tgccatttgt tcgctccatc gatctctgcc gcttcataat acgagcctgg gatctctttg
     8941 atacctgcag caaagatgat cattgccgaa ccgaattgcc aagcagacat caaaacaaga
     9001 gggatcatga ccagtttttg atcgctaaac caggaaagcg caggtgcgcc taatcgttcc
     9061 aggaaaatat tcactaaccc gtttttagaa aacaactccc gccaaactag agcgatagaa
     9121 atactgccac cgatcaaact aggcacataa tacaaagaac gaaagaaagt cgttcctttg
     9181 atattcctcg ttaaaagata agcaatcaat agaccaaaga tcagtttcac aggtacagat
     9241 gcaaatacat agagaaaagt gactcggatg gagttcaaaa aacgggcatc actcagcatc
     9301 tttatataat tatccaagcc gatccatgag actggatttc ccaaacgata atcggtgaac
     9361 gaataataaa gtgacaagat catcggtatc actgttatca atagaaatcc taggatgaat
     9421 ggagaagcaa atacgtaacc gaccacattg tcttgatcca agaaccgttt tagtttttct
     9481 tttttcatgc cttcctcctg ctgctatcct agaatgtttg acgcaaactc ataaagactc
     9541 tctgctgctt cttctgctgt tacttcttca taaatgactt tttcaactaa aagattgtat
     9601 tggtcttgaa tttccggatt caaagcacta tcaaagacat tcacttcgcc gttgtccata
     9661 tcggaaatca tttctaaata atcaaatgtc gcttgcaaag ttgcatcatc tgcacgagcg
     9721 agctgttctc gaatattcga catgattggt acaccacgtt ctccttttaa aatttcattg
     9781 gcttcttcac tgttttgaaa gaaatcaata aacttagcag cttcttcttt atgggctgag
     9841 ctttttgcca tactcagcat ttgcgaagaa cgtaaaggga taccagattc tccatctgct
     9901 tttctgcgag gtggattaat caactgaatc tccatatctt caggagcccc tgacaatatt
     9961 tctgtcaact ggttacttga tagaaacacc attgccgctt ctctatccct aaccgcctga
    10021 aaagacagat ccgtcgtact tgcagctcca ccacctggtt ctggatgggc ccctttgtct
    10081 accagatttt tacgcagctg gaaaaaatct gttagatagt ctggattttc aaatcccaag
    10141 ctgttagata gatcttcccg attataaaaa ttcaatcctt tctcaacttg tggtatttga
    10201 ataatacagc cagctataaa atcttcgaat cttgaaatag catagattcc taattcttca
    10261 gtgatttgat agctgatttg ttcaaattca tcccatgtcc aatttgaagc tggttcaggg
    10321 atatttaatt gttcaaatat atttttattg taagcaatcg caaacgaatt caccccgtta
    10381 gacagtccaa ctaattgccc gtcgtaagtc gtcgttgcta aataggattc atctgcatta
    10441 gaaatatcaa ttatcccttg ctccacatat tcatctaatg gttcaatctg attgatgtat
    10501 tgaggaaatt gattgttcag ttggaaaatg tcccagatat catctgatgc tgctagtgta
    10561 cctaattttg tgtaatatcc ttcataatca taaaattcat aatcgatcgt cacatgggga
    10621 tttttttctt cataaagctc gattactttt actgttgcat cgtgtcggac ttgcgaaccc
    10681 caccaagcca tgttcagcgt gatttttttg tcatcttctt gcccacaagc cgtcaagaag
    10741 aataacataa cacttaaagc taagatgatt ctcattcttt tcttcattct ctgcacctct
    10801 acttccattg ttatttttca atatactgta tttttataca gaacatattt attaaacgat
    10861 ctttttcaag gggaataaaa accaagactt gccttatcgt tctgcctagg atgaacaact
    10921 caatactttc aagaaaaatt agatagtgct gattcttctt tcatcgctaa aaacacttct
    10981 tgcttcaatg atgggaggat aattacgcaa agaaaaaagg atatcagaca actgcccaat
    11041 atccttgaca tagctaaata agcatgcttc ttcgtgtgcg ctcttcccta aaaaacagaa
    11101 aaataactag taattctgta tgaaattagg tacaatatac acgtcgatgc acaagcattg
    11161 actatggcaa cttgattggt cagttcccgc tgacttttca agccccgaca atttagctgc
    11221 tactaaattg tcgggtgcct tttttagttt ttattcgttt ttcattctat agaaagcgct
    11281 tcaataaatc aagacatttt tttgttagaa tgctaataat aacaaaaggt tttaactctt
    11341 cttatgtaca ttacaggtaa aaaaacaaaa aaatttctct tttttttgaa ttctgaacaa
    11401 atagtactgg agtacaaaag acgaggaaga gccactatct gctcttcctc gtcttttgat
    11461 tctcacctgt tattcatgat tctttttctc agtttcccga tatttttcca ttttacgact
    11521 aaaaagctct ccgctacttg gtggcgtata tgtgatggca tttgctcctg cttgaatggt
    11581 ctcagcaata ctttcttccg ttggaccacc tgtcgcaatg ataggaagtt caggatattt
    11641 tttacgaaaa tggcgtactg cttctgcagt ttctttacct gcactgatat tgataatcga
    11701 aataccagct gctaactttt catctatttc tgtatatttc gacgttaccg tactgataac
    11761 tggaatatca actactcgac aaacttcttt tactgtctct aaaggcgtag gaccgttcaa
    11821 aacgacccct aacgaacctt gcgcttctgc gaacatgctc atgtaggaag aacgagcacc
    11881 ttgtgtcaaa ccaccaccta caccggagaa aactggaatg tcagctgcct caataatgct
    11941 tttagtaatc gctggatgag gtgtaaaagg atacacagca attactgcat tcgcatttgt
    12001 attacgtatg atcgcaatat ccgtagtaaa gatgatcgaa cggatcaatt ttccaaatat
    12061 cttgatcccg cttgcttcta aaatgatttc cggtactctt acgatatctt ttctcaattc
    12121 cgtcatgatt tctggcgtaa attgttctgc catcagccac ttctcctttg ctatctccgg
    12181 tcatactgat tcaattgata cgtctcgatg acctggatga ttttttgcgc ataaccgggg
    12241 tctgtcgcat aacctgcatc ttgcaatgct tgtgcggctt cctgatagtt ggtggccgta
    12301 atcacttttt catacagcgc aggattccaa tctactcctt ggacgaacag ttgtgtatgg
    12361 tcatccatgg actgttccca tgaatcatac actttgaagt ctccttggat agttatccat
    12421 tcttcgttaa caaattcctt tgtttccagg cttactttct tctgatcccc ataagccttg
    12481 atcccgaaaa gattattgta tttgctagcc aaggtacttt ttccccaatt tgattctaat
    12541 atagcttgtc ctaaaatgat actaggaagc actccataac catcttgtag ttcttgggca
    12601 tgcggaacaa gccgttcaat aaattgctga tgggattgcg cttcattttc ttgctgatta
    12661 tcagtaaaag gagaagacag attcttcaag gaaaaaacaa aggctaagag tatcactgct
    12721 acaccggcaa ccactgctgg ccaacggata ttcttgcgct tttgcttttt ccactttttt
    12781 ttcgccatga tcaattccta cttttcattt aattcattca gatattcttc tagcgtcaat
    12841 tggtgtttgg tgatatactc agcgttttct ttaccgacat aacgtaagtg ccaaggttca
    12901 tatgtgatct tagttatatc ttcgtgtcct tttggataac gaacgataaa tccatactgc
    12961 gcagcattat ctgcgatcca tttagctcca ggctgatctc cgtaacttgc atccaataca
    13021 gtagaaggat aactgttgta ccagttttga tcgacgacat cgactgccag acctgtgtgg
    13081 tgttcgctgt atcctggctc cgtaatcgtt tctttagtaa tctttgtcgc ctcctcttct
    13141 gatacacctt gacgactcat cacatcttgg acattttgtt cgaaaacttg ttgttgggat
    13201 gatacagacc ggtaagctga gaccatcact aatggaaatc cagctgcttc agcagccttg
    13261 gcaaatgctt catattctgg agcaatgcgt ttgtcgatca agtatccgtt acttaatgat
    13321 gccaattgag tagactcatc aatttctttt tctaatttgt gatctggacc aaccaaaact
    13381 agcttccaat cgtctgtttt aacatctggc aattgtttgg acgtacgatc ttttgtttct
    13441 ttgtccttta ccttgctgct ggttgtttga ctgttctttt ctttactagc agttgttttg
    13501 gtgcttgttc gttcgactgc ttgattgccg gcttgaacat gcgaatcatc atcgctaaaa
    13561 ataacatagc ccattgctgc gatcataatc acaactgctc caaatcctaa aaatttattc
    13621 acttgaaaaa tcctttcgtt gttcttcacg ttccccattc gcctcatgaa cgtaagtttc
    13681 ttccagattt tcattcaccc aatcgagaag ttcgattttt tctccttcga tctgtttttg
    13741 aacatctgct ggcagacgaa aattcaaaat atcatcatag ccccattcat catattgaat
    13801 acttactttc aagtcaaggt atctaccagc ctgctgatta tttttcaaag ggatcagctc
    13861 gacttttgct gctttgctgc taagccattt ttgcgcgatg cgtgttttga tttgcttgta
    13921 ctctgcaacg gcaacgtttc gtttttcggg aaaaataatc gtttgacgca aaatcttttg
    13981 cgttagcatc cattcataat cagtgaaaag attttttact ttctgcatcg cttcagatgg
    14041 cagatttttc tcgccatctt tccatttttg ccattgttct ggactgatca gcaagtattc
    14101 atctaaaaaa tttttttcag tttcaaactg ttctaataat acactcataa taagatcaat
    14161 atatacttga tgcacgttta tcccctcctt ttaactattc tacttaagtt ttgatcaaaa
    14221 agcacctctt aaatcgctgc tttcattttt tttaatgttt caagtcctct cctctttatt
    14281 cttcgtgatt tttgttataa taatagacag acaagaaact caatccaact atgaaggagc
    14341 gttttcagat gaaaaaatat caatggggaa tcgttggtct tggtaccatc gcacatgagt
    14401 ttgccgaaag cttcaatcaa gagacgagcg aactaacagc agttgcttcc agaacttcag
    14461 aaaaagcgga aaacttcgct caccgttaca atattccaaa agcttacgga agctatcaag
    14521 aaatgttaga cgatgctgaa atcgacatcg tgtacattgc tgtaccaaat agacagcata
    14581 tcgaccatat cttggcagca ttggaagcag gcaaacacgt tctatgtgaa aaagccatca
    14641 cgatgaataa aaaagaactg gctgacgcta tgcgcttagc agaagaaaag aatttgatct
    14701 tagcagaagc gatgacaatc ttcaacatgc cactttatca gcagttacgc tcaataatgg
    14761 atacaggaaa actgggggct ttaaaaatga tccaagcacc tttcggcagt tacaaagaac
    14821 cagaccctaa aaaccggttc ttcaatccag aattagctgg aggagctctg ttggatatcg
    14881 gtacgtatgc tgtatctttc gctcgtttct ttttgagctc tcaacctgaa gtagttgcct
    14941 ctacaatggt tccttttgaa acaggcgtgg acgaacaatc cgtcactatc ttgcgtaaca
    15001 aagaaaatga acttgctgct gtcagcttga cttttcaggc aaaaatgcca aaagtcggta
    15061 tcgttgcctt tgagaacggt tatgtgacga ttgctgatta cccgcgtgct gatcgtgcgg
    15121 agatcttatt tacagatggt acaaaggaat tcatcgaaag cgggaataca tctgaagcaa
    15181 tgaattacga aataaagaat atggtacaca tgattgaagg tgacttgccc aatcgctctc
    15241 tcttcttgac aaaagatgtc atcgaaatct tggatcaaat gcaagttcta tggaaaaata
    15301 tgtaatttta a
//
LOCUS       DACEPD010000066        14996 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.069, whole genome shotgun sequence.
ACCESSION   DACEPD010000066 DACEPD010000000
VERSION     DACEPD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.069"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="IW797_001989"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="IW797_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ1224767.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="IW797_001990"
     CDS             complement(323..892)
                     /locus_tag="IW797_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ1224768.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="IW797_001991"
     CDS             1078..1980
                     /locus_tag="IW797_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1224769.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="IW797_001992"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="IW797_001992"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ1224770.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="IW797_001993"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="IW797_001993"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ1224771.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="IW797_001994"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="IW797_001994"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ1224772.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="IW797_001995"
     CDS             8548..8733
                     /locus_tag="IW797_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ1224773.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="IW797_001996"
     CDS             complement(8795..9991)
                     /locus_tag="IW797_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ1224774.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="IW797_001997"
     CDS             complement(10161..11597)
                     /locus_tag="IW797_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ1224775.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="IW797_001998"
     CDS             11890..12699
                     /locus_tag="IW797_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1224776.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12885..14882)
                     /locus_tag="IW797_001999"
     CDS             complement(12885..14882)
                     /locus_tag="IW797_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ1224777.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4377 a         3178 c         2661 g         4780 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tgacaatttt tgtcacaacc ttttttgccg agtaaacggt gttcaaaagc
    12781 cagtttctcg gtatgaagtc aaatttaagc aaaaatgtga gaagctattt tcacaaaatt
    12841 tttcttcata ctcgaaacta agcggctttt tcacgacctc ttttttattt tgccatgaac
    12901 atatcataat acgcgtccat tatttcttta gcaatcgtca agtttatgtg gtcttgttcg
    12961 tctaataagt tcggcaagac aacactgatg gcaatttccg gattgtctgt tggtccgtat
    13021 gccacgatat tactattgac tgtcgcaata tctggatgac cttccgcaac cgtttctgca
    13081 gtccctgttt ttgcggaaag gtccattttc gcactggcta atggtttggc tgttgtgtaa
    13141 ggatcggtac catggacaac ttggtggaat ccttcacgga ggatcgccat attttctgca
    13201 gaaatattga ccgtattttc tactgttgtg ccaatttctt tcttcacttc acccaagttt
    13261 ccattttcgt cattgccata gatcgctttg actaggtgcg gctgtactcg tttcccgcca
    13321 ttagcgactg ttgaagcata ttgagctaat tggattggtg tatatgtgtc aaactgtcca
    13381 aatgccaaat cggtaaagtt acctggagca taccattctt tgccattatc cgcattgaac
    13441 ttcttcatat aatcaacaga acgtgagatc ccaggagatt cattcggcaa atcgatccca
    13501 gtcgttgtac caagaccaaa ctctttgaaa gagtctctca attgctgata agctttagct
    13561 tcttcatcta aactaggtag tcccattcca ggtgtatagt ctagtcctaa catctttaac
    13621 gccactttga tcatatacgt attagaagac aattccgatg ctttgactgc atcaagagca
    13681 acttgtccgc ttcgattaaa gacagagctt ttttctgaag caccttgcag acgaatcggc
    13741 tcatcaatca aaacttgatt tccgctgatc gcgttacttg cccagcctgc tgtcaaagta
    13801 ccagccttga ctacagaccc tggatcaaag gcgctagtaa tcgtgcctaa agcattctcg
    13861 gtgatttctt tggaaccttg ttcatgactg aaaccagtca ttgctagcac ttctcctgtc
    13921 tgaggattca tagccactgc ataagcacca ggcgaatact gtgctttgcc gttgttgatc
    13981 aatgtttgat aattcctttt caaaatttct tctacttttg attggaattc tgcattcata
    14041 gataacatta aattagaacc tttttcccca gagaagattt ctttttgatt cgaaacattt
    14101 ccttcattat caagggaaac ttcatattgg gttttggttc cttgcaggac atcttcatat
    14161 tgtttttcca aataactttg tcctacacgg tcattccgag aataaccttt tgctaggtat
    14221 ttctctgctt catctgctgg aagtccttgc ttttcagtcg tcacactgcc aagaatactt
    14281 ttcagtgaac ttgctgcatt atattctcgt gtccagtctg tgccagtcga aactcctgga
    14341 agttcagaag cgcgttctgc cacaacagct aactcttgat cagtaacacc agagtttttg
    14401 atatagacag tgtttaattc atatgcacta ttcatccgtt taaagatggt agcaattttt
    14461 aattgctcct cgtttaaatt aatctcatca gctgtgactt tattgaccat cgcactatat
    14521 tgttcgctgt tccctaattg cttttctttt gccgaaagtc tttttgtggc ttctttcaaa
    14581 tgtttgggat cagccagcca aaaatctttt ttatctctat ctgtcagatt ttcatctacc
    14641 ggaacatcga tcagcgagtt cagcttcgtt gcgatttcaa gcaaatcttt agccgtaact
    14701 tgtgttcctc tcgtatatgt gatggcctga ttcgctttat tcgtaactaa agcttttcct
    14761 gtggagtcgt agatcattcc gcgaggagta ctttcttgga ccgtaatcgt agaagtagac
    14821 ttcactcgcg cctccatctc ttcgccgttc acgatctgca aataacccaa tctagcaatc
    14881 aacgcaacaa ataacgtaaa tataatgaaa aaaagaaaat ttaagcgaaa aggaacatgg
    14941 gaccggcgaa acgttttgtt cttaggttct tgtgcacctt ttcttatttt ttctaa
//
LOCUS       DACEPD010000067        14549 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.070, whole genome shotgun sequence.
ACCESSION   DACEPD010000067 DACEPD010000000
VERSION     DACEPD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.070"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            4..816
                     /locus_tag="IW797_002000"
     CDS             4..816
                     /locus_tag="IW797_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ1224778.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IW797_002001"
     CDS             813..1613
                     /locus_tag="IW797_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ1224779.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IW797_002002"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IW797_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ1224780.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IW797_002003"
     CDS             3110..4819
                     /locus_tag="IW797_002003"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ1224781.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="IW797_002004"
     CDS             5031..9389
                     /locus_tag="IW797_002004"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ1224782.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="IW797_002005"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="IW797_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ1224783.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="IW797_002006"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="IW797_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ1224784.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="IW797_002007"
     CDS             11227..11520
                     /locus_tag="IW797_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ1224785.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="IW797_002008"
     CDS             11517..11828
                     /locus_tag="IW797_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ1224786.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="IW797_002009"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="IW797_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ1224787.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="IW797_002010"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="IW797_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ1224788.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4959 a         2583 c         3238 g         3769 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaat
//
LOCUS       DACEPD010000068        14482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.071, whole genome shotgun sequence.
ACCESSION   DACEPD010000068 DACEPD010000000
VERSION     DACEPD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.071"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(287..649)
                     /locus_tag="IW797_002011"
     CDS             complement(287..649)
                     /locus_tag="IW797_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224789.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IW797_002012"
     CDS             complement(723..1103)
                     /locus_tag="IW797_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ1224790.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IW797_002013"
     CDS             1185..2360
                     /locus_tag="IW797_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1224791.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="IW797_002014"
     CDS             complement(2437..3021)
                     /locus_tag="IW797_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1224792.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="IW797_002015"
     CDS             3222..4220
                     /locus_tag="IW797_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1224793.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="IW797_002016"
     CDS             4332..4841
                     /locus_tag="IW797_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ1224794.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="IW797_002017"
     CDS             complement(4871..5530)
                     /locus_tag="IW797_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ1224795.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="IW797_002018"
     CDS             complement(5532..6296)
                     /locus_tag="IW797_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ1224796.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="IW797_002019"
     CDS             complement(6304..6981)
                     /locus_tag="IW797_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ1224797.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="IW797_002020"
     CDS             complement(7018..8403)
                     /locus_tag="IW797_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ1224798.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="IW797_002021"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IW797_002021"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IW797_002022"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IW797_002022"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ1224799.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="IW797_002023"
     CDS             9342..10199
                     /locus_tag="IW797_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ1224800.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="IW797_002024"
     CDS             complement(10280..10615)
                     /locus_tag="IW797_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224801.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="IW797_002025"
     CDS             complement(10682..12052)
                     /locus_tag="IW797_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ1224802.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="IW797_002026"
     CDS             complement(12130..12666)
                     /locus_tag="IW797_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1224803.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="IW797_002027"
     CDS             12975..14456
                     /locus_tag="IW797_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1224804.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4461 a         2883 c         2353 g         4785 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 gctatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taaaaaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta tt
//
LOCUS       DACEPD010000069        14144 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.072, whole genome shotgun sequence.
ACCESSION   DACEPD010000069 DACEPD010000000
VERSION     DACEPD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.072"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(51..3704)
                     /gene="rpoC"
                     /locus_tag="IW797_002028"
     CDS             complement(51..3704)
                     /gene="rpoC"
                     /locus_tag="IW797_002028"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ1224805.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="IW797_002029"
     CDS             complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="IW797_002029"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ1224806.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8128..9096
                     /locus_tag="IW797_002030"
     CDS             8128..9096
                     /locus_tag="IW797_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1224807.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9114..10019
                     /locus_tag="IW797_002031"
     CDS             9114..10019
                     /locus_tag="IW797_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1224808.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10016..10822
                     /locus_tag="IW797_002032"
     CDS             10016..10822
                     /locus_tag="IW797_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1224809.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10979..11947
                     /locus_tag="IW797_002033"
     CDS             10979..11947
                     /locus_tag="IW797_002033"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ1224810.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12264..12788)
                     /locus_tag="IW797_002034"
     CDS             complement(12264..12788)
                     /locus_tag="IW797_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ1224811.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13075..14144
                     /gene="scm"
                     /locus_tag="IW797_002035"
                     /pseudo
     CDS             13075..14144
                     /gene="scm"
                     /locus_tag="IW797_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
BASE COUNT         4006 a         3050 c         2793 g         4295 t
ORIGIN      
        1 caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt
       61 tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca
      121 ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta
      181 ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc
      241 ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat
      301 aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt
      361 gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct
      421 aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc
      481 atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa
      541 acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac
      601 aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct
      661 tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct
      721 ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct
      781 gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca
      841 cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc
      901 gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg
      961 acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt
     1021 tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt
     1081 tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca
     1141 ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg
     1201 atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct
     1261 tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg
     1321 gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt
     1381 tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca
     1441 ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca
     1501 cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt
     1561 ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg
     1621 tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca
     1681 ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata
     1741 ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt
     1801 tctgttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg
     1861 aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca
     1921 aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg
     1981 aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt
     2041 tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt
     2101 gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag
     2161 accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc
     2221 atatcttgag aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca
     2281 gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc
     2341 atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt
     2401 gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt
     2461 gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg
     2521 tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga
     2581 acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca
     2641 cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca
     2701 cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga
     2761 gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc
     2821 aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca
     2881 ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca
     2941 ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt
     3001 ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat
     3061 gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc
     3121 aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta
     3181 atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga
     3241 tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact
     3301 acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga
     3361 cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca
     3421 atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca
     3481 caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt
     3541 gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag
     3601 ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa
     3661 gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat
     3721 ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg
     3781 gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct
     3841 tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt
     3901 gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc
     3961 tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc
     4021 aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct
     4081 tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa
     4141 gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca
     4201 ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa
     4261 cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca
     4321 gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg
     4381 ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat
     4441 actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat
     4501 acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt
     4561 gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta
     4621 tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa
     4681 acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt
     4741 ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact
     4801 tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt
     4861 accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata
     4921 cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg
     4981 cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata
     5041 tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca
     5101 tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg
     5161 ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa
     5221 tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca
     5281 tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat
     5341 tcaacgacac cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat
     5401 tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc
     5461 atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
     5521 gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat
     5581 acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct
     5641 tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag
     5701 tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg
     5761 ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt
     5821 gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga
     5881 acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt
     5941 ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa
     6001 ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc
     6061 aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc
     6121 ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag
     6181 tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa
     6241 gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc
     6301 acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt
     6361 ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca
     6421 atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg
     6481 atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt
     6541 ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt
     6601 ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct
     6661 ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt
     6721 ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag
     6781 atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt
     6841 gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct
     6901 ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca
     6961 tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg
     7021 atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct
     7081 ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag
     7141 tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct
     7201 acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt
     7261 tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt
     7321 ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat
     7381 tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc
     7441 gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc
     7501 ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc
     7561 ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc
     7621 ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa agtcaacact
     7681 tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc
     7741 aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc
     7801 attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa
     7861 taacttttac tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta
     7921 aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag
     7981 acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt
     8041 tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg
     8101 tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct
     8161 gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat
     8221 ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat
     8281 aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc
     8341 gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc
     8401 aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat
     8461 gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta
     8521 gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc
     8581 gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg
     8641 tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa
     8701 aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg
     8761 acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca
     8821 agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc
     8881 gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg
     8941 ggcaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt
     9001 cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta
     9061 ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa
     9121 atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga
     9181 ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct
     9241 ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag
     9301 cttgtcttct aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca
     9361 caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg
     9421 tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc
     9481 attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt
     9541 ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta
     9601 ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata
     9661 tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg
     9721 cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg
     9781 cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga
     9841 ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg
     9901 ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc
     9961 cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa
    10021 tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa
    10081 tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg
    10141 tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga
    10201 tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc
    10261 gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt
    10321 agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt
    10381 caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga
    10441 aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt
    10501 attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct
    10561 tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa
    10621 tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact
    10681 gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct
    10741 cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga
    10801 tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct
    10861 cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga
    10921 aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat
    10981 gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga
    11041 aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt
    11101 ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga
    11161 cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt
    11221 acaaacgatg ttgacttctg aatctacgat gaaagatgcc aaactggcag cagctcaagg
    11281 agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg
    11341 acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga
    11401 caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc
    11461 cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga
    11521 tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat
    11581 cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc
    11641 tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa
    11701 tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc
    11761 gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa
    11821 aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca
    11881 aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac
    11941 gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga
    12001 attctcttca aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa
    12061 aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa
    12121 aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat
    12181 cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg
    12241 taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc
    12301 acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg
    12361 actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt
    12421 aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga
    12481 ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct
    12541 atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct
    12601 aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat
    12661 ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct
    12721 tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa
    12781 taatccattt attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata
    12841 cctacttata tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat
    12901 agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat
    12961 atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga
    13021 ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa
    13081 aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg
    13141 gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact
    13201 ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca
    13261 actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat
    13321 tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact
    13381 gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc
    13441 gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg
    13501 cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat
    13561 ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca
    13621 gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact
    13681 gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat
    13741 acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt
    13801 tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca
    13861 gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca
    13921 atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa
    13981 aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg
    14041 gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt
    14101 acagtacaga tacatctacc acaagtgata gctcagattc atca
//
LOCUS       DACEPD010000070        13715 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.073, whole genome shotgun sequence.
ACCESSION   DACEPD010000070 DACEPD010000000
VERSION     DACEPD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.073"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(119..775)
                     /locus_tag="IW797_002036"
     CDS             complement(119..775)
                     /locus_tag="IW797_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1224812.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            968..1246
                     /locus_tag="IW797_002037"
     CDS             968..1246
                     /locus_tag="IW797_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224813.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1265..2473
                     /locus_tag="IW797_002038"
     CDS             1265..2473
                     /locus_tag="IW797_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1224814.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2621..2986)
                     /locus_tag="IW797_002039"
     CDS             complement(2621..2986)
                     /locus_tag="IW797_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ1224815.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3133..3906)
                     /locus_tag="IW797_002040"
     CDS             complement(3133..3906)
                     /locus_tag="IW797_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ1224816.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4080..4940)
                     /locus_tag="IW797_002041"
     CDS             complement(4080..4940)
                     /locus_tag="IW797_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ1224817.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4940..6358)
                     /locus_tag="IW797_002042"
     CDS             complement(4940..6358)
                     /locus_tag="IW797_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1224818.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6415..6864)
                     /locus_tag="IW797_002043"
     CDS             complement(6415..6864)
                     /locus_tag="IW797_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1224819.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7309..7572)
                     /locus_tag="IW797_002044"
     CDS             complement(7309..7572)
                     /locus_tag="IW797_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ1224820.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7671..8966)
                     /locus_tag="IW797_002045"
     CDS             complement(7671..8966)
                     /locus_tag="IW797_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ1224821.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8963..10234)
                     /locus_tag="IW797_002046"
     CDS             complement(8963..10234)
                     /locus_tag="IW797_002046"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ1224822.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10399..11268)
                     /locus_tag="IW797_002047"
     CDS             complement(10399..11268)
                     /locus_tag="IW797_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ1224823.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11560..13170)
                     /locus_tag="IW797_002048"
     CDS             complement(11560..13170)
                     /locus_tag="IW797_002048"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ1224824.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         3887 a         2895 c         2340 g         4593 t
ORIGIN      
        1 tgttcaatca gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt
       61 ttccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttgttcatc
      121 aaaagataat attgtcttct aacaagtcca tattcaaaat aacgatatga tggtctttaa
      181 cggtgataat accatctttt ttcagttgtt ttaaaattct gtttacactg ctagcagttg
      241 agatgccaca aaatttggcg atttcttcat tcgtgatcga aaaatcgatc agcatccctt
      301 cttctgtccg cacaccgaat aaatcatata attcgtaaag ttgcgtacaa acagctccca
      361 gctttccgtt taatagcatt tgctgcattt ttttcatcga ataaagcaga cgatagcggt
      421 aatactcttt tacatagtgt tgtagctttg ggtcttggtt gatattttgc caaaagcgca
      481 cacgattgat tcgataaaac tctgcatatt ctgattcaac tcgaatatta tacggcgaat
      541 cgatgaattg tgaatattcg tcttttaaaa gagagacgat ttcaagatct ttgatatagc
      601 gaaggttaaa ttctctccca tcatttgaaa taacacttgc ttttaccgtt ccctctttca
      661 agatatacgt atgggaatct tccatccctt cgaatgtcaa atatttatga tatcctttgc
      721 gtataatcgg aaaatcattt ttatccaaat attcctttaa gactctacta tccaatgatg
      781 ctcccccatc caaactttca tctaaaaaga tcatacagta ctttaaaact ttttaaagtc
      841 caaaggtatt ttactaatga tattaacatt tgttaagtac ctttttccat cacttatgcg
      901 atttttttcc ttttttttat tcgttatact gcagaagtaa aattttttaa gaatagaggg
      961 aaaaaacatg aattactgga tgaacacaat cattaatcga ttagaaacag catatcaaac
     1021 aagatttgat atgaaagctt ctttagtctt tttaaatgat gcgtatcaaa actcgatcga
     1081 attgatcaaa gcagtggatg agaatccaac aaacgaatgc gaagaatttt taaatctgtt
     1141 tatgtctaca agagatttat ttattcgcca acttgttgat cgctatccat ccaattatca
     1201 cgatgtagaa gttcaaatac aaaaattaaa agcatattca gcttaagggg aatgtataac
     1261 atacatggaa aagaaattat ttaataaata ttttattgga atcacattga tcaattttat
     1321 tgtctacctg atttattatc tgttgatggt catcatggca gtcattgcgc aagacacctt
     1381 acacgccagc ttgagccaag caggttttgc ttctgggatc tatatcatcg gaacattgct
     1441 ggcacgctta ttcatgggga aacagttaga actatttggt cggaagttta ccttaagagg
     1501 cggcgcgatc ttctatctcc tttcaacatt agcatatctt tatacgccaa ctatcggggc
     1561 attgtattta gttcgattct tgaatggatt cggctacggt acagtttcta cagctacaaa
     1621 tgctatcgtt actgcatata tccctgcatc taaaaaaggg gaaggcatca attattatgg
     1681 attaagtact agtttagctg ccgctgttgg gccattttta ggaatgattt tgctgaatat
     1741 gactgatttt cgtttcatta tctggttttc aattgtactg gtcttctttg ttacgattgc
     1801 ctgccttgtc tttcctgtaa aaaatattcc attatctgaa gaacaaaaaa tatctcttcg
     1861 ctcatggaat atcaatagtt ttattgaaaa gaaagcactt ttcattacag gaattgcctt
     1921 tttgatgggt ctttcttatt caagtgtttt atcattttta tcttcttata caaaagtaat
     1981 ccacttagtt gctgctgggt catttttctt cgtcgtatat gctttggtca tcacatttac
     2041 acgtccattg actggaagga ttttcgatgt caaaggagaa caatacgtga tgtatcctag
     2101 ctatttattc ttaacagcag gattattttt actgagtgta acgacgaata gtgtgacatt
     2161 gcttatctct ggagcattag tcggcctagg ttatggtacg tttatgtcaa atggacaggc
     2221 tgtttgttta aaaatcgtcc gcgaacatcg aatcagtatt gctttatcta cctactttat
     2281 tggtttagac ctaggcttag gcgttggtcc ttatttgatg gggatgctaa aatctcttac
     2341 ttcatttaga ggattgtatg tgattgccgg agtcattcca ttgatatgta cagcactcta
     2401 tcttgttaat ggccgaaaac atagtcatga agcagagggc gaattgattg aatcattgaa
     2461 aaatgaaatt tgatcttgat gaatagataa aaaaaaccgt tcagatctga aaataatctg
     2521 agcggttttt tgagtaaaaa tagtttatga ttattcttaa ttaaacatct atttattttt
     2581 aaagctttca tttatcgata agaatagttt aactgttgat ttattccatt ttccgttcat
     2641 ctggtacgat gccttgactt agcaaccact tgtggaagaa ccattctgcc acaccgatca
     2701 atagagcagg aatcaatact ttcatccaaa tatcgactgt atcattcata aaatatagat
     2761 agagccacaa gataactgca gctgaaccag catctgcgat cgtcgctgcg atgttcccta
     2821 ctttacgtaa gatcaacaga tcccctagca catagatcat gactacaata attaccccaa
     2881 caatcgttga atccataaag ctaacaccgt ataaacctgt cagcacgacc cacagaatcg
     2941 cccagatcat gattgccttc actaccaacg ccttcacatg tttcattttc tgcactcctt
     3001 tctcctggat aaaaaagtaa gattatctaa caagataacc ttttcattat tataccatcg
     3061 gctgagagaa gcgagcaaag ataaggtcct atctaaaaat gaggaagcca cttagaacat
     3121 tcacgtaata atttatccgt acagacattc gatatggtgt tcctttactt tcgcatactg
     3181 ttcttttgtc agttctttac ttgtcaccag ataatcgatt tcatgaaaag caatggcttg
     3241 atgcatgaat ttatgtgcaa ttttttgctg tgtaactaaa acggcaagcc ggtctgattg
     3301 ttttctaaaa gcttttttga ccattgcctc tccttcatga gtttctgtta ctccttggct
     3361 gaaatctagc ccgcgacaac tcattatcgc taaatccgca taatatgaca atgcatcgtt
     3421 ataggctttt gaaccgttga ttgttgctcg tttcggaaca attgcccctc ccaatacaga
     3481 tacttttgcc gttgtatatt cactcaacat agcagctgtg acgatgccat tcgtaatgat
     3541 ctgcaaacca tcaaatcttg ctaggtttct gactaagtgt aaagaggttg tactggaatc
     3601 caagaataag accatatcat ctcgaatcaa agaagaagct gtatctgcaa tcttacgttt
     3661 ttctgtttct tcttctaatt tggtgatctt catcaggtaa ggcacgtcaa tttttcgatt
     3721 gtcaataagc atggcgccac cgcgaactct acggatcaat ccactttttt ccagaaaaac
     3781 aaggtcacgt ttaatcgtgg cttcgctata atttactaac tcgatcaaat cggtgactct
     3841 tgcaaattgg ttgtttttta gataattcac aatctcctgc tgtctattct ccacttcatt
     3901 caacataaaa tcaccccatt tactttatat agtaacagga aaaaagaggt tgtgacagaa
     3961 gtgattggct tcgagaaata agaagaaatt tttcgaaaaa actacttctg aacatcgtgt
     4021 gtggttaaga gggtatgaca agactattgt cataccctct tgaccacaca tttttatttt
     4081 tagtataaac tagccttgcc tactgtgttc aaaatttcca ttttatgttt gaccacttct
     4141 tgtacctttt tattagctgc tggataaatt gcattcggct cgtacatcga tggattttca
     4201 ctcaatattt ctctcaaagc attgaaaaag acgcttttaa gatctgagct caaattcact
     4261 ttggcaactc cgtattttac tgcttcgctg acttctgcgt ccggattacc tgaaccgcca
     4321 tgtaaaacaa atggaagatc gatttgttgg ttcaattctt tcagtaagtc gatatttaat
     4381 tttggagttc tgtttttcgg atacaagcca tgcgctgttc caattgctac agcaagtgta
     4441 tcgattcctg tttctttcac aaaaatcttc gcttgatcag gatctgtata aatgattgtt
     4501 tcagcacctc cttcagcaga accattattc ccaatcgttc caagctcagc ctccactgag
     4561 acatttaccg catgagctaa cgcaaccact tttttcgtaa tctgtacatt gtcgtcgaat
     4621 gatttcaaag acgcatcgat catcacggaa gaatagccgt ttcgaatcgc ccgtaagaca
     4681 tcttctatcg taccgccatg atccaagtgg ataacgatcg gcagtttact tgtataagca
     4741 taggctctta ctgccgcaac aaactcatca cttaaatagg cgatttcatc aggatgaatt
     4801 tctaagatta ctggtgcttc taatctctct gcttcttcga tgattgcttt catcatatca
     4861 tagctgcaaa tattgaaggc tggtactgca aattgattct ctttagccac tttcaataac
     4921 tctttcattg tatataacat tacgcttcca cctcatttgt tagattttca tttttcgtcc
     4981 acttgtcttg ttttttctct ttcactgttt ttttcaatag tccaagcata aaggctgtag
     5041 tgactgtacc tgatgcaatt gccaagacat acataggtaa attgttcata acatttggaa
     5101 ttagcatcac ccaaatccct ccatgaggtg ccattgaagt aactcctact gccatggaaa
     5161 tccctgcagc gactgctgaa cctgtcatca aactaggtat cacgcggatc gggtcagata
     5221 ccgcaaacgg tatagcacct tctgtaatat acgaagcgcc taaaatccag catgattgac
     5281 ctgaagtccg ttcttctttg gtaaatttat gtttgaataa taaagtggct aaagcaatac
     5341 ctaggggagg tgtcattcct gcagccatac aagcagcaat cggtgcattt actccagctg
     5401 acatcaaacc gatcgagaaa gtcgaagtcg atttattgat cggcccaccc atatcagctg
     5461 ccatcatgat cccgataagt aatccgaaca atagcccgga tgtatttccc aagctattca
     5521 gccaatttgt caaagcatcc aacacccatg tgatcggttg ttcaatcaca aataccatca
     5581 aaagcccaat aattaagatc gataaaaagg ggacgacaat cagctgataa atagcagaaa
     5641 attctttttt tacgctaatt ttatttgcca gaaaatcaca gatataccct gctagcacgc
     5701 caccaatcat tccgccaaga aaacctgaac cacctgtaga ggccagtaat ccggcaacaa
     5761 ttcctggggc aaaacctgct tttccagcaa tcgatgtagc aatccccgcg cctaacgcag
     5821 gaaccatcat ggcaaacgct gtatctcctc cgatccttga aaatgcagct gctaacacat
     5881 tatagtctgc tgagttggga tcagccgctg taatcccaaa ggcaaaactc agtgcgatga
     5941 tgattccgcc tgcgatcaca aatggaagca tgtaattcac accattcatg aaatgattat
     6001 agattgtcgg ctttttctca tttgcagctg gttctttttc attagacaca acgaatacgc
     6061 caatatctct caatgcttcg tctattacta cttgcggttc tttgattgct cggcttgtag
     6121 agacacgctt tacttttttc ccggcaaatc ggtccaattc gatttcttta tctactgcaa
     6181 gaatgattgc atctgcctct ttgatttctt gttcagataa ctgattttct gtttttacac
     6241 cttgtgtttc gaattttgct tgatggttta gatttcttgc tgttacttct aatttttctg
     6301 ccgacatata agtatgagca attccagtcg ggcaagcagt tacaccaacg attttcatga
     6361 ttattttcct cctttttctt cactatcgat actcggacaa agactctact attttcattc
     6421 ttgactaatc tgtctgatga tatctgctgg cgtagtggct tcttttattc cctttctaaa
     6481 atctgcattc atcaatcgtc tggagagtgt gctgagaatc tctagatgca tatctgctga
     6541 tttttcagga actacgatgg caaaaatata ttcaattggc atttcatcta tcccgctata
     6601 gtccgttaat ttggctgtat gaaaaaaaca cagtgctggt tttgtcacat atttgctttt
     6661 tgcatgggga atcccaaatc catcttctat tcctgtggag atctcctcct ctcgtttgaa
     6721 tagtgcctta acgacttttt gaacatttct taccttattt gtttttttca acacatcccc
     6781 tattcgctca aataatgatc cttttgaatc tatcgatgga tcgaaaacaa tcagttcttc
     6841 tgcgatcaat tgatcaaacc tcataaaaaa cctctccccc acaaaaaatg ttaacgtttt
     6901 cttggaactg aggtcagtat agcatcgcta gctgtaccgt tcaatattat gaatgaacag
     6961 aggaaaaaga cccatcatcc gcgtgtataa gtcattaaaa tgaacgatct agctcacaaa
     7021 caatcgcaag tctataacaa aacaaaaagc cttccttgaa aggaaagctt agcttctaat
     7081 tttaggtaaa tggtttttta cttcgaaata acccctcttc atttcgttaa tataaggtgt
     7141 ttttatcaag tggataatta aaactatata ctagtttttc attgttttgt gcattatgat
     7201 cctacgactt tttcagcatc aaacgaagtt ttaaattggc aaaaaacata aaaaaacaga
     7261 ctatccctat cagatagtct gctaaacgtt gagttatcag cgtttttatt attccgcagc
     7321 gtttgcgtct ttgagaccgt attttttgtt gaaacggtcc acacgtccgt ctgcttgtgt
     7381 gaatttttga cgtccagtgt agaatggatg tgagtcagaa gttacttcga cacggatgac
     7441 tgggtatgta ttaccgtctt cccattcaac tgtttcttgt gaagtttttg ttgaacctga
     7501 caagaatttg aaaccagttg ttgaatccat gaatactact gggtggtaat ctggatggat
     7561 gttttctttc atgatttttt cgctcctttg ccctgctcca tttgctgaac cagagttgat
     7621 ttgtcgattt acaacattaa catcttaaca tgtttcagtc aaggttgcaa ttatcttttc
     7681 agatttctgg tcggattttt tttaccaaag gagacatctt gaaattcttt gaaaaattgc
     7741 tgattgtttt ttgttttacg caaaaagcgg atcagttgtt ccgtatattc taatgaatct
     7801 ccagtcatat tttttcgaag tttccaaatt tcttctaatt cttcacttgg catcaataat
     7861 tcttcttttc gggtacctga ttttttgata tcgatggctg ggaagatccg acgttcagat
     7921 aattcacgtg aaagttgtaa ttctagattt ccagttcctt tgaattcttc atagatcacg
     7981 tcatccatac ggcttcctgt atcgactaaa gcggtcgcta aaatcgtcaa actgccacct
     8041 tcttcgatgt ttcttgctgc cccgaaaaat ttcttaggtt tatacaatgc ggctgggtca
     8101 atcccgccac tcaatgtacg tccgcttggt ggaacgacta agttataggc ccgagcaagt
     8161 ctagtaatac tatccatcaa gataacgaca tctcgtttat cttcgactaa acgcattgcg
     8221 cgttccaata ctaattcaga aactcgagta tggttttgcg gctgcagatc aaatgtagaa
     8281 gataccacgt cgcctttcac gcttcgttct agatccgtca cttcttccgg tcgttcatca
     8341 atcagtagaa cgattaattc tacttccgga tagttttcgg tgataccatt tgcgatttct
     8401 tttaatacag aagttttccc tgcttttggc ggtgcgacga tcaatccacg ctgaccaaaa
     8461 ccgattggag aaaagatatc gatcattcgg gtagatagtc tttgcggtgt tgtttccagt
     8521 tgaagctgtc tttgcggata aagaggtgtt aaggctggaa aatgcggacg ttcttttgct
     8581 tcttccggat cttttccatt aacgctttcc acgtgcatca aaccataata acgttccgat
     8641 tctttaggtg gacgggcttt tcctgccact ttgtccccat ttctcaaact gaatcgtctg
     8701 atttgtgagg cagaaatata gatatcttct gcacttggtc cataattgat tggccgtaaa
     8761 aaaccgtaac catcttgacc aacgatatcc aatattcctt ccatataata aaatccttgt
     8821 ttctctgctt gtgcacgaat cacagctaga gataattcct ttttattcat cttactgtaa
     8881 taaggaattt taaattcttt ggcataacta taaacttctt ttaacgtttt gttctctagt
     8941 tctgccattg tcaaataatc gctcattcag ctcccacttc ctctatcatt tcaattgatg
     9001 cccctaaggc tgttagtttc tcgataatat gatcataacc gcgtaagata taatctacat
     9061 tgtaaatcgt cgttgtccct tctgccaata gtccagcaat cactaaacaa gcacctgccc
     9121 gaagatcaga agccacgact tctgtgccgt gaagtttatt tggaccattc acgatgatca
     9181 tatttccttc cacggtaata tcagcgccca ttcgtgctag ttccggcaca tggttcacac
     9241 gtttcgcata gatcgtatcg atgatttctg aagtgcctgt agtcatcaat agtaacgcag
     9301 taagtggctg ctgcagatct gtggcaaatc ctggataagg ataggtcatg atgtttgcgg
     9361 gttttaattc tttcgagggg taaacttcga tttcatcttc gctgattttc atatggacac
     9421 caatttcctg caattttgct ataaagcttt ccaaatgttc aaagatcaca tttttcactt
     9481 tgataccatt tcctactgca gctgctaatg ctagataggt tcctgcttcg atgcggtcag
     9541 gaatcatgaa atgccgacag ccatgtaatg tttcgactcc ttcgatacgg atcacatctg
     9601 tacccgctcc tcgaactttt gcccccatat tattcagtaa agttgccaca tctatgattt
     9661 ccggttctcg tgcagcgttt tcaattactg tctgtccttt tgcttttaca gcagctaaca
     9721 tcacattgat cgttgctccg actgaaacca cgtccatgaa aatccgattg ccacgcaatc
     9781 ctttattttc tgtccgtaaa tacatggcac catgttcatt tgtcacttca gcacctagag
     9841 cttcaaatcc tttgacgtgt aggtcaatcg gtctagggcc taaataacag ccacctggca
     9901 ggcctactac cgcttcgcca aatttaccaa gcagtgttcc cataaaataa taagaagcac
     9961 gcaaactatt gatttttccg tgcggcatcg gaatcgatac gacttctcga ggatcgattt
    10021 ctagtatgtt gttggaaaaa tggacagttg cgcccatgat ctctaaaatt tcaattaatg
    10081 aatgaacatc ttgtatgtcc ggcacacctt ccaagatcac tggggaatca gctaagattg
    10141 cagctggaat caatgcgact gcactatttt ttgcaccgct gatcgttact tcaccagcta
    10201 aaggtcggtt tcctttgatt acgatttttt tcatctgttt ccaccttgtt tattaatctt
    10261 ttttcatgga tatgcttgta ttttatcata gataaaaaaa agaaacgagc atggtctgcc
    10321 gttttaagta aaaaagaatg gagagcgact tcaaaagtcg ttctccattc ctatatatcg
    10381 tatctgttat gtggatgctt acgctttgtt agctgaaccg aaccagtcga tatgagattt
    10441 agcatcttca acgattgctg ctttacctgg agctaataat ttacgagggt cgaatccttt
    10501 accttcttta tctttaccag cttcgatata tttacgagta gctgcagcaa atgataattg
    10561 gaattcagtg ttaacattta cttttgagat acccattgag atagcttttt cgatttgatc
    10621 ttgaggaata cctgatccgc catgtaatac taaaggcatg tctggaccta cagcatcagc
    10681 aattgcttgt aagtggtcaa aagctaatcc tttccagttt tctggatatt gaccatggat
    10741 gttaccgata ccgcaagcta agtagtcgat acctgtagca accatttgtt tgcattcttc
    10801 aatatcagct aattcgccgt taccgatgat tccgtcttct tctccgccga ttgaaccaac
    10861 ttcacattca actgagatac cttttgcatg tgctttttca acaacgtctt tagctaattt
    10921 caagttttct tcaaatggta gatgtgaacc atcgaacatt actgaagtat agcctaattc
    10981 gatacattcc aaagcagctt cgtaatcacc atggtctaag tggatagcaa ctggaacagt
    11041 gatgttcatt gcttcaacta aatcagtgat taaatcttta cagattttgt aaccacccat
    11101 gtatttagct gcacccattg atgtttggat aagtactggt gcttgttttg cttcagccgc
    11161 ttcaaggatt gcttgagtcc attccaagtt gtttgtgttg aatccgccga cagcgtaacc
    11221 gcctttacga gcagctttta aaaattcagc tcctgatact actggcataa taaaattcct
    11281 cctaagtttt gtaaaactaa atttgttaag acaattctta accaacgacc ctattttacc
    11341 aaacatttcg gtagttttct actaaaatca atgaaaatga aaattttcaa gaagtttttt
    11401 gcaaaaaata aaatatccaa tagcgcttta atcgaaaaaa gtcttttgct ttctatcttt
    11461 tttcctacaa ataataacaa atcagaaaaa gagaaaagtg acaagaaaat tgcttatccc
    11521 cttctcttgc tacttttctc tttctatcaa gttcaaaaat tattttgttt cgtgatctgc
    11581 taatgatgct ccgacaaatg ctttgattaa tttttgcggg cgatttggac gagaaatcaa
    11641 ttctgggtgg aattggcatc ccacaaagaa ttttttctct ggcagctcta cgatttctac
    11701 taaacggtta tctggtgaga cacctgaaaa gactagccct tgttcttcaa acaattgacg
    11761 atatttgtta ttgaactcat agcggtgacg gtgacgttct tgcacaacat cggctccatc
    11821 atatgcttca gccgtcttcg ttcctttttt gattttacat gggtataatc caaggcgtaa
    11881 agttccaccc aaattctcga tgttttcctg atcagccatt aaatcaataa tattgtttgc
    11941 tgtatctgga ttcgtttcag ctgatgacgc atcttctaat ccaacgacgt tacgtgcaaa
    12001 ttcgacacaa gccatctgca taccaagaca aatacctaaa aacggaacgt cattttcgcg
    12061 agcataacga atcgcttcga ttttcccttc tactccgcga tcaccaaatc caccaggaac
    12121 caagattcca tctgcgtctt tcaaacgttc ttctacattt tctctcgtta attcttgtgc
    12181 tttgatccaa tcgatctcga tgtctgaatt ataggcgaaa ccagaatgtt tcaatgcttc
    12241 aactactgag atatatgcat ctggtaactc gacatattta ccgacaagcg caatccgtac
    12301 ttttttcttc aagttcaaga cacgttcttc taattctttc cattctgtca tgtctgcttc
    12361 tggtgtatct agttttaaat ggtcgcaaac aatttgatcc attccttgtg cttgtaaaac
    12421 taatgggatg gagtaaagtg tatctacatc tggcgactcg ataactgctt ctggttcgac
    12481 atcacaaaat tgtgccaatt tgtttttcac gctttgagaa acaggttgct ctgtacgtac
    12541 caccaaaata ttcggttgga tacctaaacc acgtagctct tttacactgt gctgcgttgg
    12601 tttagttttc atttctccag cagctttcaa gtaaggaatc aatgttgtat ggatatacat
    12661 aacattatct gtaccgacat ctgctttcat ttgacgaagt gcttcaagga atggcaaaga
    12721 ttctatatcc cctactgttc cccctacttc tgtaatgatc acatctgaat cagtcatttt
    12781 agctgctcgc atgatttttt ctttgatctc atttgtaata tgaggaataa cttgaacagt
    12841 tgctcccaag tattcgccct tacgttcttt acgcaaaact tcagaataga tttttcctgt
    12901 cgtcacgttg gagtatttgt tcaaattgat gtcaataaaa cgttcgtagt ggcccagatc
    12961 caaatctgtt tcagcaccat catctgttac gaatacttct ccgtgctgat atgggctcat
    13021 cgtccccgga tccacgttga tgtatggatc aaatttttgg atagttactt ttaagccgcg
    13081 gtttttcaat aagcgtccta aagaagctgc aacaatccct tttccgattg atgaaacgac
    13141 accgccggtc acaaaaatat attttgtcat aagttcaaaa actccttttt aatttatgtg
    13201 caggaaaagt ctcagacaat aaacaaagct ccctattctt caataagaat agggagccga
    13261 aatttctgta aacttctgac cctctaaaag ggtgcccaag gtgtataata caagctgact
    13321 aagggaaagt caagtactca aatgaatact atccgcttac aattttttct ttttcgatat
    13381 cctttgagaa attatttctt aagtccttta tgagtgcaaa aaaaatttaa cccacataat
    13441 aagcttgata taaacatagc taacgttaaa cctaagcaaa tcagaaaatc gttactgaaa
    13501 aattttaagt gaaataatag ataaaaaaat aaaacaaaca ttatgataga taatatccca
    13561 cctaacaaag aactactttt tcctacttgc agcaacagtt tatatagctt atcgtagaaa
    13621 caatcttttt ctttcaccct tatttacctc ctttacgtaa aaaacgtagt ccacagttct
    13681 cggatttttg gcatatcttt tctattaaat aatat
//
LOCUS       DACEPD010000071        13693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.074, whole genome shotgun sequence.
ACCESSION   DACEPD010000071 DACEPD010000000
VERSION     DACEPD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.074"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(221..1177)
                     /locus_tag="IW797_002049"
     CDS             complement(221..1177)
                     /locus_tag="IW797_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ1224825.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1281..1883
                     /locus_tag="IW797_002050"
     CDS             1281..1883
                     /locus_tag="IW797_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1224826.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1971..3470)
                     /locus_tag="IW797_002051"
     CDS             complement(1971..3470)
                     /locus_tag="IW797_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ1224827.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3486..4010)
                     /locus_tag="IW797_002052"
     CDS             complement(3486..4010)
                     /locus_tag="IW797_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224828.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4308..5057
                     /locus_tag="IW797_002053"
     CDS             4308..5057
                     /locus_tag="IW797_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ1224829.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            5048..5833
                     /locus_tag="IW797_002054"
     CDS             5048..5833
                     /locus_tag="IW797_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ1224830.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5802..6518
                     /locus_tag="IW797_002055"
     CDS             5802..6518
                     /locus_tag="IW797_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ1224831.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6620..7639)
                     /locus_tag="IW797_002056"
     CDS             complement(6620..7639)
                     /locus_tag="IW797_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ1224832.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7658..8716)
                     /locus_tag="IW797_002057"
     CDS             complement(7658..8716)
                     /locus_tag="IW797_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ1224833.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(8937..10085)
                     /gene="nagA"
                     /locus_tag="IW797_002058"
     CDS             complement(8937..10085)
                     /gene="nagA"
                     /locus_tag="IW797_002058"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ1224834.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(10161..10997)
                     /locus_tag="IW797_002059"
     CDS             complement(10161..10997)
                     /locus_tag="IW797_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1224835.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(11181..12398)
                     /locus_tag="IW797_002060"
     CDS             complement(11181..12398)
                     /locus_tag="IW797_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ1224836.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            12781..12864
                     /locus_tag="IW797_002061"
     tRNA            12781..12864
                     /locus_tag="IW797_002061"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12815..12817,aa:Leu,seq:gag)
     gene            complement(13104..>13693)
                     /locus_tag="IW797_002062"
     CDS             complement(13104..>13693)
                     /locus_tag="IW797_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224837.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         4140 a         2794 c         2365 g         4394 t
ORIGIN      
        1 tggacttatt accgcagagg aaagcttttg aacaccgttt aagaaagagg ttgtgagcct
       61 accgttcagc tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgaa
      121 tattgtgact tgtgaccgag gagttgacag gagaacaccg tttaaagaag aggttgtgaa
      181 atcagctttt tgacctgaat attagctgaa ttcacaacct ctatttttgg attttttgtt
      241 gggagaattc tgccttgatt tcaataaaac ttttaaaaga ctgcaaaaac tggaacaatt
      301 ctgctcgatc ctcgaattca gggcgactag tcggcagagg catttgccga tacaatacat
      361 aaacttcttc aatacgtgtt aaaatttgtc tgccatcatt tgactctgaa aatgtttctg
      421 cagtataaat caataaacca tagatatgtt ttccataata agctggttgc tggattctct
      481 ctagattttc cagcatgtct ttaatcacat tggcctgtgc tcttctcata gagaagtaag
      541 tttcgtagta tagcggctgt ctgagccagt agttttcttg atgttctctg gcatcttctt
      601 ggctttcgcg aatgaatgtc agtaaatgtt cgcatttgat ctgcacatct tctttatttt
      661 ctgataaaag aaattctgcc atttgtctca aaatatttcg aaattctttt tcgatctgta
      721 tttggttttc cttcagctgc ttcgtacgat ctggcatgta aacatttgca agcaaagcaa
      781 atactaatcc aatcgccatt aatcccgctt cattgccgat cagctgccaa gacatgtttt
      841 cctccactaa ataatgagtg accaaaaccg aatttactac tatcccttcg gttaatttga
      901 agcgagaggc aagcgggatg aacaggaata aaaaaagact gaagccaaaa atagtatacc
      961 caacagcaaa aaaactgaac agtgctgttg ccgtagctaa tataaaggct gcgattcgat
     1021 tcatgcccgt acgcaatgta gagcgcttgg tatttcctac acttaataat gcaatgatcc
     1081 ctgcagctgg ccaatacaac agcgataagc tacccgctgt gaccatcgaa aagaagacac
     1141 tgatcactgt tttgactgtt cgcattccta tacgcattta cttccccctt ttttatttta
     1201 attatacttg aataatgaaa aactagctat cagacaaaag tttaggtatg atagtaaaaa
     1261 gaccaaaaag gagaatctct atgcaatttg gccgttttcg actaggtatg cgtacgatca
     1321 aaagtgcctt agctgttttt ttatgtatct tgttttttca tttgactcaa cgaggaatcc
     1381 cgatgattgc tgcattatct gctgtttttt ctttgcgtca ggacttgact acaaccgttt
     1441 cttttggaaa gtctaggatc atcggcaata cattaggtgg tacattagct atcatttatt
     1501 tttttgtcaa aagctatttt cacaatgatt ttttggtaga attgatcttg cttcctattc
     1561 tagttattat tgtgatcgtc gtgtcagatg gtattaacaa taattccggt atcatttccg
     1621 caatagctac gttgttgctg attgcgttga gcatccctca aggcgaatcc gctttgtatg
     1681 ctatccaacg tgtactagat acttttatag ggacattcat tgcaatcggt atcaatttct
     1741 ttcttcgtcc tcctgaaaca gaaaagaagg aagaactatc agaagatttg gtcgaactcc
     1801 aaaagaaaga acgattgcta agaacaaatt tacaagagat agaagctaag atccaaaaac
     1861 aaaagaaaaa tagcgaggac taattttctt ttgcaataaa ataaaaaaat ggagagaaga
     1921 actttaaaaa gtcttcctcc atttttgatt gaacggtgaa gtgtactact ttattcttcc
     1981 ttcatcgagt tttgttctgc ctcttttggc gcttcataca tatttacttg gctagtagtc
     2041 ccacgtgtcg acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt
     2101 gtttcttgag tagaagaaag caaatttgtg gcataatttg tcactcggtt cgctccactg
     2161 gtttctcctg aactgcttcc tgtatcgaca acagagattt tttcgatatt tcctagcggc
     2221 tgtgctgctt cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa gacagctgct
     2281 tcgccatatt ccttgaaggc attggcgatc ttttgttttg cttcggcttc tgcttttcct
     2341 tttgctagtg ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct
     2401 tctgccagtg cttccacacg gaatcgttct gcttcggctt cagtcacttc tttgactttt
     2461 tgagcttgtg cttcttgttc tttagcgtac cgatcggcat ctgctttttt cttcacttcc
     2521 gaatcatatt gcttttcacg acgtgtgatt tctttttctt ccagttcgat ttgtttttga
     2581 cgttctacga ttttaatctg catttcctgt tcgattactt gttgttgtgc tcgggcactt
     2641 tctaaattgt aggcttggtc tgcttttgct ttagcaacat cttgttcttg tttgtaggtt
     2701 gctaatttca gttctttttc ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca
     2761 gctgcttgtg attctttttc tgcttgagct tttttgatac gcgtttcttt tagagcttcc
     2821 gcttctgcta tatctgcatc tcttttgact tgggcgattc ttggtttccc taaagagtca
     2881 agataaccgt ttttgtctct gacctctttg atagtgaaag aaacgatgat caaccccatt
     2941 tttgcaaggt ctacagaagc gacttcttgt acgctttgac tgaacttatc gcgattttga
     3001 tagatttctt ctacagtcat agaaccaaga atcgagcgca agtgtccttc taatacttct
     3061 cttgcttcat tttccagttc ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca
     3121 atctcttcta cagatgaacc gattttgatg atcgaagtac catcacacat caccggtacg
     3181 ccttgttccg tatacacttc aggggtggag acgtccaatt tactcgataa tagactgatc
     3241 cgattcgaac gttgaaaaac tggcaagacg aatgcgccac ctccacgcac aattttgatt
     3301 ttgttattgc tttcatcaat gtgcacattt ttgcttccta aataacttcc actgatgatc
     3361 aacgcttcat ccggttttgc tgtctggtac ttcgctacaa agacgatcag caacatcagt
     3421 aaaatcaaag ctgcgattgc gatcgcgata aaaacaactg gtatttccat ttaacacacg
     3481 ctcctttaag cttttatcat gctttcataa ggcatgacat aacagattct ttccttgatc
     3541 tcaatgatca atactttcgt tccccgctca gctgtctgtt cttgggggct gtaaaaacaa
     3601 gctggacgac taatggaacc ggtaacactt ttcaactgaa tttctcccat acctcgtaca
     3661 gggatcggtg tgatcacaac agctacttgt ccttccagag ttttttcaga agacgacatc
     3721 gttgattcgg catgtttcat cggcatcaat atatagaaat taagcaaaaa taccaacact
     3781 atcgatatgc ctccactagc aataaataaa aataaagatg attgatctgt taaacgctca
     3841 cctagaaaac caaataagct tgtgaaagtt aaccaaggga taataagcat cggatcaagc
     3901 gggccatcaa aatcgaatac atctccaaaa ataatcaata ggactgccaa tcctgcacaa
     3961 atcagcaatg tataccaata aatcgtctca agtgcgattc cccctatcat atacacgcct
     4021 ccttccattt ctacgtctat cataccaatg tttgaaaatt ctgtatagct tcttttaccg
     4081 actcagtggt gttttttata ggaaggttgt gtttatcgtt gacaatttgt taaatttttg
     4141 ttttcatgtt aaaattttca caataaattc tttcgaggta gcctatgttt ccttttttta
     4201 agaaaaaacc attaaagctc cagaacacga tcatccttgt cgtttgtttc tccacattcg
     4261 tttctttgat tgtttctgcc atattgatca ggaattttat tctttcgatg aaccgatcta
     4321 gtgtgtcaga taaaatctct agcattgcta aaatcgttgc caatgataaa ctcatccaaa
     4381 aaaagctgca gactggtgac ccgacacatg cgattcaagg aataacaaat gaaatcatga
     4441 ttgcatcgaa tttagatttt atcgttgtca tggaccctga aggcattcga tattctcacg
     4501 cagacccaag ttacatcggt cttcctttct caagtcatga agatgtgaaa aaagtgattg
     4561 gaggacaaga atttttctct gaaaaagaag gaatgttagg gttcggcttc cgctatttcg
     4621 tccctattta ttatgatcaa gagattattg gtgcagtttg tgttggctta acaaacaaca
     4681 cgattgacaa tcaagtcaaa gatggacaaa aaatgattgt tttttcattg attattggac
     4741 ttagcgtagg catcataggc gccgtttttc ttgcccaaaa aatgaaaaaa gtactgcttg
     4801 gacttgaacc acaagaaata gcagaacagt taagtgaaaa aacgattatc gaaaatgaag
     4861 tagatgaagg aatcttagcc atctcttctg ataaaactat cctcttgatc aatacacctg
     4921 caaaaaacat tttctaccaa acagagcagc tattggattt cgaagtagga aaaaaatcag
     4981 tgataatcta tatgacgttt tgtttaaaga gacgattgag catcaaacga ccgttcggac
     5041 tcaatccatg tatttaaatg ggatagaaac tatttctagc gttacacctc tttttagcaa
     5101 tgaccgtttt ttaggagcag tcgcaacttt acgaaatcga tcagaagtaa tgaaattaat
     5161 ggaggagctt ggggggacga aacaatatat aaactccatg agagctcaaa cccatgaatt
     5221 catgaataaa cttcatgtca tttctggttt gattgatttg aagaagtatg aagaagtcac
     5281 ccattttatc cagcagctaa atcaaaatta taaaaaagag attggccaag tctcaagtct
     5341 gatcaaagta cctgcaattg ccggtttttt attaggcaaa ataaacgaag ccaaagaaca
     5401 atctgttgaa atgctgttag aaagcgattc ttttatccct aaaatggaga tggatgactc
     5461 catccattca cttttgcaag tgttagggaa cttacttgat aatgcaaaag aagccgtgct
     5521 acaaaaacat gaaagaaacg gacaggtccg gattcattta aactatgaca cagaagctga
     5581 agtattcatc gtgaccgtct gcgatgacgg tgtcgggata tcagaagaaa tctcagaaaa
     5641 aatatttacg ctaggttttt cgacgaaggg aatcaatcgt gggtttggat tgaatctcat
     5701 ccattcaatc gtcaaacgcc acggtggatt tttagaattt tccaaacaaa caaatggcgg
     5761 tacaatcgtc tatctagaat taccaaaaaa ggaagatcaa aatgatgaca aatgtcctga
     5821 taatcgaaga tgatccaatg gttgcagaat tgaatcgtac ttttgtggaa agcatcccta
     5881 atatgaacgt aatcgaaagt ttcagaaatg tagaagaagg aaaaaaatat ttcagacaaa
     5941 aaaacgcaga tctgctgctg cttgatgtat acatgcctgg cgaaagtggt atccattttt
     6001 tatcttggct tagacagcaa ggaaatgata tcccagtgat tttgatcact gctgcagatg
     6061 atatcgaaac cataaagaat gtgatccgct acggtgcctt agattatttg atcaaaccgt
     6121 tcactttgca acgttttcag ctggcgatcg aaaagtatct tgctttctct cagacaatcc
     6181 aaacgactga aaaagccgat cagaaattga tcgatcaagt attcaaccac accactccca
     6241 ttcacgaaat accatcattc atcgatttgc ctaaaggtct gtcaaaactg actttaaaaa
     6301 aaataagtgc atttattcta aaagaagata ctattttttc aacaaaagaa ctggctcaaa
     6361 aaagctcact gtcacgtata tccgtcaaga aatatttgaa ttttttagta gaacaagata
     6421 tattgaacga aagaatcaaa tatttggaaa tcggccggcc gctaacctgt tatgaattga
     6481 caaagaatgg gcggaaaaag ctcacagatt actgttgagt catcgaatta tcccatacaa
     6541 aatagtccaa gtatacagaa gtaacaactg tttacttgga ctatttttaa acattttatt
     6601 gacgaatatc catgttttct tatcttaatt gacgggcttt aggaagagat acctcatttt
     6661 tcgtttttcc agtttctatt atttcacgaa agttctcgaa acttgtcgaa atcatatttg
     6721 ataatgcctc atctgtattt gaacctacat gcggggttac aagaactcta gggtacattt
     6781 ctactaattg ctggatagca gggtctggaa tcgtctccca tggcttgaag gatcggaaaa
     6841 aaacctctgt ttcatttgca aaaacatcgg ttgcaaaacc agctaaatga ttgcttttca
     6901 aagctcttaa aatagcttca ttatcttgaa gctctccacg ggctgtatta atcaagatgc
     6961 tacctttttt catttgagct aaaaatcctt cattgatcat ttgatcgttt tcacctggga
     7021 tatgaggtac atgaatactg acaatatcgc ttttggctaa taactcttca agcgagtgga
     7081 acgtcaatac ttttttagca gcttctgtct gatgaatatc gtagccgatc actttggctc
     7141 ctagtccctt aaacaaaaca gcttccgtca accctatcct gcctgttcct atgatcccta
     7201 ctgtgcaatt tctgatttca cgactaaaca tttgctcatc cactcgaaaa tctttgaaag
     7261 atgttcggat cgtcgtatac gcagtatttc tcaaaagcat catggcaaaa gtcaaagata
     7321 actctgctat cgcatttggc gaatacgatg gtacacgggc tactgtcata ccaaaatcag
     7381 ctgccgcttg tagatcgata tgattgactc ccacagtccg tgtaaaaaca taacgaatcc
     7441 catattcctg catttttgca agattggtct catctgctat acaatttccc ctcaacaata
     7501 cagcatcatg gtcctttgct gacacgatat tgtcatgtgt caacagttct tcataaagac
     7561 tgagatcata atggtaaata ttcaattttt caaaaaaatc tatctcgttc ggacgaacac
     7621 cgtaacatgc gattctcata ctttcttcct ccatttctta tgaaaataaa cctatgccat
     7681 gaatcatttg taatacaacg atccatacag gtgtcagtac cacagcaaaa atagttgaca
     7741 gtaaggatgc attggaggct aaaagtgcct ccctatcaaa actgatagca taggctgctg
     7801 ccactgttgc agctggtgta gccatcatga taacgattgt tgccagtgct tcaaaattga
     7861 ctggtagaat attcgttgcc gtcaaaactg ctagaatcac gatattcaat gcaggtacaa
     7921 ataccacttt gactagactg taataccatg aagttttttc agatgcagct tttttgaagc
     7981 ttacttcacc taatgttgcg ccgattgcta gccacgccaa tggtgaactt aaaccagcta
     8041 aataatccat cggctgaaat agccacggtg cagtctggtc aattcgcaaa aacgctactt
     8101 ggcttatctc tccttctgca cctgtaactg acacttgtgg cagataccct tggaacagcc
     8161 aaatgaaaag gccggcaaaa gtagcgataa tgattggatt gacgaacatc gctttgatat
     8221 ttttagcttc catcttcaag ccactcattt ttatgtatcc ataagaatac aaaaagattc
     8281 ggtagccaat attgaaaatt gaggaataca tcacgccgac aggaccataa atcgcactga
     8341 cgataggcgt tccgaaaaag gtcgtagaac caaagatcgt caggacacgc aacgttgttt
     8401 gcttatcccc tttatagctt aagaaaaaag gttttgaaac tagaataaga ataatataaa
     8461 taactaatcc ccaaatcaag acattgattc cttgcatcag taattctgta ttgatatctt
     8521 gcatgaaagc tcgaaatgcc agtgctggca aagccactgt taagacgact ttcgatagaa
     8581 tttttccaac agattcagaa aaaatcccct tttttctaca aacaaaccct aataatataa
     8641 taaaaactgt tgaagtgatt gcactgacaa tcgacatatc tgtcaaagtc gatcgaaaaa
     8701 tgtcaaataa gttcatcttt ctcctcctaa ttatgttcat ttattcacaa taaaaaaagc
     8761 ttttattttg ctaatacgta ttaacatttt taattttatc tttacattgt tacttattga
     8821 acaataggaa attaaagaaa cttttgaaac ttatgtaaag tgatttttac tagaacacaa
     8881 aaaaaacctt aaaaccaaac ttaagaagtt tagttttaag gttaacttaa attatattat
     8941 gcgtggaaac gttccacgcc atctagataa gttgcttcca atgtcatatc agggttcaat
     9001 acgataaagt ctgcatcgcg tccttgagcg atttttccac atgtatcatc gattttacag
     9061 ctgattgctg gaatcagact agccatcatg actgcttgtt ctggtgttgc taatccccag
     9121 tctacaacat ttttaattgc ttctttcaat ttcaatatac ttccagctaa gttgccagat
     9181 tctagacgtg ctgttccttc ttttactact actggaaatt cgcctaaatt gtaatttcca
     9241 tcaggcatcc caccggccat catacagtct gtaattaatg cgacatgatc atgtcctgct
     9301 ttttccatta ggattcccgc agcatttgga tgtacatgat ggccatcaca aatcaattca
     9361 gaaaagacat gttctaaaga taataacgca cccaccatac ctggttcgcg atgattcaat
     9421 ccacgcatac cattgaaagc atggacaaat acacttgcgc ctgcttcaac agctttttgc
     9481 gcttcttcta gtgtagcatt actgtgtcct agagaaacaa ctactccttc ttcagtcact
     9541 tgtttgacaa actcttctac accttgacgt tccggtgcta aagcaatttt tttgattaaa
     9601 ccacctgaag cttcttgcca ttcatggaaa gtatccaaat caggatcccc gaaataactt
     9661 gggttttgtg cccctttgtg ttcttctgtg aagaaaggac cttcaaaata gattcctttg
     9721 attttagctc ctggtgcttc ctgatacatt ttgccgattg tctcagctac atcttttaaa
     9781 cgttctttgc ttgacgtcaa tgttgttggt aaaaatgaag tcactccaca agataacaga
     9841 ccttcagaca tcactttgat gccttctgca tcattatcca ttacatcatg attcatatag
     9901 ccgtgaatat gtgtatctac caaaccagga gcaatccatt tgccgctttg gtcaatgatg
     9961 ttgacttctc cctcaggtag ttctttggtg tattcaccaa aaagtccatc ggtaatttca
    10021 agatagcctg catgtttgac acctgacgct aaaaagaatt tgtcagcata gataaaagtt
    10081 ctcattctat tctctccctt tgtttttcta cctctaaagt actcttattt agagaagaag
    10141 tcaagaatgg tctacaccat ttacaaattt tttgaaattt gtttcacagc ttgtgctaag
    10201 ccttcataga atgctgctct ttctggttct tctttacgct tgtatattaa gcttttaagc
    10261 acaaataaat cattgaggta atagtagcta ttcttctctt gagcccaata gattccttga
    10321 ttcacctgtt ctaacgcttc gtcgatttca ttttgatgga ataagaaatt tccataatta
    10381 taaaaaattt gcgaaaattc actttttgat cgttcatcag ctgattgatg cagcagttgg
    10441 atgcttcgac tcaaatagta gcgtgcttca gccgtttttc ctgccacacc atatattttt
    10501 ccgatacaac taatcagttg tatttctgtg tcagaaacat ggattctttc tttttggcta
    10561 gagtagctta atgcttcttt caaataataa agggactgag agatattttg acgcaacgtg
    10621 aattcgcaaa caccgagata ataataatag cgttgaaagt cagttgcttg atacagattt
    10681 tcagaaatat cagaagattt tagtacttgt tctaacttcc gataatcacg tttttggaaa
    10741 caactatcca acagttcaaa taatttatcg tttgtccgag tagtttttcg agagagattc
    10801 atcacatgat caatcgttac ccctagccgt tgacaaatct gcatcatcac caatacattc
    10861 ggcagttcct cattattttc gatccgactt aatacacttt gtgaacaaat atcatgcgaa
    10921 agcatttttt gcgtcatttt ttgttctttt cgaatctctt taatgatcgt tccaaaggac
    10981 tcgattaacg tttccataag tatcctcccc aaaatatgcg tattcgcatt ctattcgtat
    11041 tttactttag gaagtcgcaa acttcaagaa aagacttgca actaaataat atactttagt
    11101 tatttaagaa aactccacta gaaagagagc ctcagtgtgt ttgtctgtgg ccctcctact
    11161 gaaaaatttg gtttttaaaa tcatttttgg cgataggttg ctaggaattc tctcattttc
    11221 ttagctgttt gtttcaaatc atccatttta ggaagataaa cgattcgaaa atgatctggc
    11281 tggttccaat taaatccacc accatgtaca agtagaatat gatgttcatg taaaaagtcc
    11341 aaaacgaatt tttcatcatc caaaatattg aatcgctttg tatcaatttt tgggaatata
    11401 taaaaagcag cttttggttt tacggctgaa agaccaggaa tatcattgat tgcattatag
    11461 atatattctc tttgttcata gatccttccg cctggcagga gcagctcatc gacactttga
    11521 taaccgccta gcgcggtttg gataatttgc tgagacaata cattggaaca caaacgcatg
    11581 gaggacaaca tattcagccc ttctatatag cctttgacgt gtgacttatc tccacttagg
    11641 accatccagc ctactcgaaa tcctgctaca cgatgagatt tggatagacc gttcagcgta
    11701 acaacgaaca aatcaggcgc taatgtagca atcggaatat gttctagacc atccatcact
    11761 aagcgatcat agatttcgtc ggaataaata atcaggttat tttgtcgagc tacttcaacg
    11821 atttcttcta ataattcttt tggataaagt gccccagtag gattattagg attaatgata
    11881 acaatagctt ttgtccgact cgttattttt gatttgatat catcaatatc aggataccat
    11941 tctgcctctt catcgcaaat ataatgaaca ggtgtgcctc cagctaatgc aatagaagcc
    12001 gtccacaacg gatagtccgg cattggaacc agtacttcat ctccattgtc cagcaacccc
    12061 tgcatacaca tcgtgatcaa ttcgctcact ccgttgccgg tgtagatatc attgatcgtc
    12121 acattcggaa aatgtttcag ttggcaatat tgctcaattg cttttctagc agaaaagatc
    12181 cccttggaat ctgagtagcc ttctgagtca cgggcattca tgatcaaatc acgaatgact
    12241 tcgtttggtg cttcaaatcc aaaaggtgcg ggatttcccg tgttcaattt taaaatacta
    12301 atgccctctt cttgcatccg ctccgcttct tctagaacgg gaccgcgaac atcgtaactg
    12361 acaccttcta atttgttgga tttttcaaat tctctcatca cttagccaat ccctcatttc
    12421 aaaaagttag aactttccaa attttttgtt atttactatt tcaatatcat actccatcct
    12481 agaaaatttt gccacttatt taggaaaaac gtaagtatta aaaaaacaca aacaagcgtt
    12541 gagaacttaa ttgtcatgat agtcattcca tagattcgct gcccaatcca ttgcaattcc
    12601 acaaatgatt cctagcataa aaccaagcaa aaaacgtccg aatgcaagtc caatcaatac
    12661 acctaagatc attcccagtc ctgaaaattc tccgtcatat ttctccatct taataagtcc
    12721 tcccattttt gagcttgaca actatgtaaa acctcgctat cattagtgtg ttattattac
    12781 gcagatgtgt tggaattggc agacaagcat gattgagggt catgtgagcg ttagctcgtg
    12841 tgggttcgag tcccatcatc tgcactacaa ctcgtgaagt taattttgct tcacgagttt
    12901 ttttgtttga acgtttcttt atgccattat atacatatca ctgtatcctt tacaaaaaga
    12961 acgtacaatc atgtaaacag cctgaggtga gtatatgaag gtcggaaaca agctactcgt
    13021 agaaacttgc aagtgttaaa acaattaatc gaaaaacgag taaaatccct cacctaatac
    13081 taaagcgagg gattttttgt atattatctg tttatcaccg ttattataaa ggtacaaata
    13141 ccaagcacgg ttaatccaaa ttgcataaat tcattttttt gtacactcat gcttttcatt
    13201 agctctttaa ctgccattag tactaagatg gcgataacaa cataaagtat atagtacgtc
    13261 cgcattccgg aagcccatac tgtgggagaa aatcccataa ttaaccttga acaaaatccc
    13321 attatcatca aaataactaa aaaaatagaa gttttcttat tgtcaaaact taaataaatt
    13381 ccaacaatta aacatagtaa taatgccaag actactaaag tagctatcca agtacctgga
    13441 taaaaaagtg acagtttcgt tcctaactct gtaaagagat tattcaggtt actactactc
    13501 caaatcattg cccttttatt tccattgacg taagtaaagc tttgacccat tgtattacca
    13561 aaataaataa tcaggtttaa gaaaaacggt aatgcagtca atatacgcac ataatagttt
    13621 tgacatttcc tatatgttag aaggaaaatt aagaaaaata gcaataaaaa taagatattc
    13681 atgtctaaaa aaa
//
LOCUS       DACEPD010000072        13661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.075, whole genome shotgun sequence.
ACCESSION   DACEPD010000072 DACEPD010000000
VERSION     DACEPD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.075"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IW797_002063"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IW797_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ1224838.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IW797_002064"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IW797_002064"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ1224839.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IW797_002065"
     CDS             complement(2169..2906)
                     /locus_tag="IW797_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1224840.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IW797_002066"
     CDS             complement(2997..3524)
                     /locus_tag="IW797_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ1224841.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IW797_002067"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IW797_002067"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ1224842.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IW797_002068"
     CDS             complement(4401..4886)
                     /locus_tag="IW797_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ1224843.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IW797_002069"
     CDS             complement(5039..5236)
                     /locus_tag="IW797_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224844.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IW797_002070"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IW797_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ1224845.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IW797_002071"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IW797_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ1224846.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IW797_002072"
     CDS             9211..9423
                     /locus_tag="IW797_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ1224847.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IW797_002073"
     CDS             9425..11104
                     /locus_tag="IW797_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1224848.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IW797_002074"
     CDS             complement(11358..11558)
                     /locus_tag="IW797_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1224849.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="IW797_002075"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="IW797_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12249..12322)
                     /locus_tag="IW797_002076"
     tRNA            complement(12249..12322)
                     /locus_tag="IW797_002076"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12286..12288),aa:Ile,seq:cat)
     gene            complement(12511..13530)
                     /locus_tag="IW797_002077"
     CDS             complement(12511..13530)
                     /locus_tag="IW797_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1224850.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(13541..>13661)
                     /locus_tag="IW797_002078"
     CDS             complement(13541..>13661)
                     /locus_tag="IW797_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ1224851.1"
                     /translation="GVNWRYTDITVMIITGFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT         3972 a         2929 c         2372 g         4388 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgacatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaactg agctaagggt actggttgtt gccacataaa gccataaaca atcaaccagt
    12361 agaatgtgtg gcaacaaacc tgttatcgca tagcttggag tgtgactatt atgggtgata
    12421 gtgaagatat gcgataacat cactatttta tcgaatgatt tttatagttg tcaatatagt
    12481 tatgtaatgc tcctcaacga ggagctattt ttatcgttta ggaatattta aataccaacg
    12541 tttgtcatgg aaatcttgcg caccgccttt agtgtttccc tctggatcat tcgttgcccg
    12601 catcatgaca tagactttct tattagggaa gttacgcatg ttaaaagata catgatagcc
    12661 aacatttcca gaagtattat aagcttgatt tacatctgat ctataaattc catcagctct
    12721 tactcgagct aattctttcc cagtattgta atccataatg aagatatact cgtatttata
    12781 gttagcaatg tgccatccag ctacatgcaa atttgcattt tcgatttctc caaactgatc
    12841 aatgtgggcg taattcgttc catctgtcag tgtaggatta gctgcaccag ctcgtgttgg
    12901 atcaatgaca ggcttgtttt cagaagttgt tggattttca tcggtaaatc catgagctaa
    12961 atcatatgct aatttttctt tacttacgcc catttcagaa agataaccgt aaggatctgt
    13021 atgatcgccc cagatgtttt gtgttaccca taaatgcgat ttgattcccg gttggttata
    13081 aggagtgtcc aacgttaatg gaataccata tttcattgct gaatctctag ccaattcaac
    13141 gtatgccttg tagtttttct caaacgttgc tttatcatgt gtgtgttgta actcaatctg
    13201 cacaggactg ttggcattag catacgaacc agcaccgtac tgtacataac caggttgacc
    13261 gacttgataa acaattccgc cgtctcccac aatataagca gtataagcgc tagtccatga
    13321 acgttgcata tactgcgctt cattgcgtcc tgttgctgtt tcattagccg tttcatgcag
    13381 taaaatatac tgattatttg ctacttgtga gctaccttca tttgcgccca aattaaattc
    13441 attgttgata gtataggcaa atccgttaat tggcaataaa aaaagagccg ttaacaggct
    13501 catcgcagta atagtaattt tctttttcat atctatcctc ctatttatct gatgaaattt
    13561 tcttatacgt tcgatttgat acacctaaaa tactccctag aaatgctcca aaacctgtaa
    13621 taatcatcac cgttatatct gtgtaccgcc agttaacacc t
//
LOCUS       DACEPD010000073        13212 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.076, whole genome shotgun sequence.
ACCESSION   DACEPD010000073 DACEPD010000000
VERSION     DACEPD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.076"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            147..1016
                     /locus_tag="IW797_002079"
     CDS             147..1016
                     /locus_tag="IW797_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ1224852.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="IW797_002080"
     CDS             1225..2250
                     /locus_tag="IW797_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1224853.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="IW797_002081"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="IW797_002081"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ1224854.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="IW797_002082"
     CDS             3333..5036
                     /locus_tag="IW797_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1224855.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="IW797_002083"
     CDS             5038..7071
                     /locus_tag="IW797_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ1224856.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="IW797_002084"
     CDS             7082..7522
                     /locus_tag="IW797_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1224857.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="IW797_002085"
     CDS             complement(7722..8849)
                     /locus_tag="IW797_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1224858.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="IW797_002086"
     CDS             complement(8852..9529)
                     /locus_tag="IW797_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224859.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="IW797_002087"
     CDS             9793..11559
                     /locus_tag="IW797_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ1224860.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="IW797_002088"
     CDS             11574..12011
                     /locus_tag="IW797_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1224861.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="IW797_002089"
     CDS             12024..13175
                     /locus_tag="IW797_002089"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ1224862.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4488 a         2293 c         2731 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DACEPD010000074        13202 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.077, whole genome shotgun sequence.
ACCESSION   DACEPD010000074 DACEPD010000000
VERSION     DACEPD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.077"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            169..1908
                     /gene="yfcC"
                     /locus_tag="IW797_002090"
     CDS             169..1908
                     /gene="yfcC"
                     /locus_tag="IW797_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ1224863.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2065..3249
                     /gene="iadA"
                     /locus_tag="IW797_002091"
     CDS             2065..3249
                     /gene="iadA"
                     /locus_tag="IW797_002091"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ1224864.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3360..3563
                     /locus_tag="IW797_002092"
     CDS             3360..3563
                     /locus_tag="IW797_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ1224865.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3664..4326)
                     /locus_tag="IW797_002093"
     CDS             complement(3664..4326)
                     /locus_tag="IW797_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224866.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4339..5841)
                     /locus_tag="IW797_002094"
     CDS             complement(4339..5841)
                     /locus_tag="IW797_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1224867.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6389..8068
                     /locus_tag="IW797_002095"
     CDS             6389..8068
                     /locus_tag="IW797_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ1224868.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8195..8674
                     /locus_tag="IW797_002096"
     CDS             8195..8674
                     /locus_tag="IW797_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ1224869.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8893..9573
                     /locus_tag="IW797_002097"
     CDS             8893..9573
                     /locus_tag="IW797_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ1224870.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9581..10912
                     /locus_tag="IW797_002098"
     CDS             9581..10912
                     /locus_tag="IW797_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ1224871.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10927..11889
                     /locus_tag="IW797_002099"
     CDS             10927..11889
                     /locus_tag="IW797_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ1224872.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12000..12338
                     /locus_tag="IW797_002100"
     CDS             12000..12338
                     /locus_tag="IW797_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ1224873.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12417..13124
                     /locus_tag="IW797_002101"
     CDS             12417..13124
                     /locus_tag="IW797_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ1224874.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4213 a         2324 c         2892 g         3773 t
ORIGIN      
        1 aatggaaaga gaaaaaaata atcatacata ggagaaaaaa aacaaaatag aaaaaagtga
       61 ttttctaata aattattcat gtggaaatgt catttttact gtataattca tttgattatc
      121 cattttttat tcatcaaaat tactgaaaaa agaaaggaag aaataaattt gaagaaaaca
      181 aattcttcag gaaaaccgaa acgcgacttc actaaattat cgacacccca tacgtatgtc
      241 attatttttg gtgtagtcat ctttgcatgg atactgactt ttgtcgttcc agcaggaaag
      301 ttcagcacgc aagatattga atacaaagat gcaaatggcg aaacaagtac tcgtactgtt
      361 ttacgtcaag attccttccg ttatgcttac gagctggata agtcgtatgt gtttgatcaa
      421 ttagaagaac tgcaagatca tccagcagaa cgtgaaaaac tggatgttcc ggaaaaagga
      481 ttggaaaaag tcattgctga cggagaaaaa aatcttacgc aggaaaaact agatgaaatc
      541 tccttgacag atgatgtgct ttacgatcag tacggcgaaa atatctatga tacttctaaa
      601 aaattgcata aaacagccaa aatatgggga acagatgatt ttggcggctt tggtttctta
      661 aattttgttt ttgaaggact agtatctgga gataagtatg gatctgctgt agggatcgct
      721 gcattgatcc ttgttgttgg aggtgctttt ggtattatta tgcgtaccgg agcgatcgac
      781 gcaggtatct atgcgtttat cagtaaaact aagggactgg agcgtttagc tttaccgcta
      841 ttattctttg ctttttcatt cggtggcgct acattcggga tggcagaaga ggtaattcca
      901 ttctcaatgg tgatggttcc atttgtcatt gcgttaggat atgactcgat cgtagcagta
      961 accgtgactt atgtcgcatc tcaagtcgga aatgcgacct cttggatgag cccgtttagc
     1021 gtagcggtgg ctcaagggat tgctggaatc cccgtgttgt ctggtgcgac attccgattg
     1081 attatgtggg tagttgttac tgcattagcc gctggttata tgatgatata tgcagaaaaa
     1141 atccgtaaaa aaccagaaag ttcgttgact tacaaatcag atgattattt ccgttctcat
     1201 atcaaaaaga catctgatga aaataaaaaa ttcatgcttg ggcataaatt gatcttatta
     1261 gagatgttag ttgtccttgt ctggatcgtt tggggcgtga ctcaaaaagg ctattacatt
     1321 ccagaaatcg cttctcaatt ctttgtgatg ggattagttg ctggaatcat tgctgttctt
     1381 ttcaaattag atggaatgaa agtcaatgac atcgcttctt ctttccaatc tggtgcagct
     1441 gatttagctg gtacagcaat cgtagtaggt atggccaaag ggattcttct tgtcttggga
     1501 ggatctgatg catctgttcc ttcagctttg aatacgattt tgcatgggat cgggacggcg
     1561 ttgaccggtg ttccagctgt cattggtgct tgggcgatgt acatcttcca aagtttgttc
     1621 aacttggttg ttacatcaaa ttcaggacaa gcagctttga ctatgccaat catggctcca
     1681 ctggcagatt tagtaggtgt atccagacaa gtagcagttc ttgcttatca gcttggttca
     1741 ggttttatgg acgcttttac acccgtatca gccagcttga ttggtgtgct aggtgttgca
     1801 cggatcgaat gggcaaaatg ggcaagattc caaatcaaga tgcaaggatt cctgtttgtt
     1861 ttaggaacta ttttcatcat gattgccatt gcaataggtc tgcagtagta ctggagacct
     1921 gtaaataaaa aagtaatcca aacagtagat aaggactcca agaaccgtca ttttacggta
     1981 aaatcaaagg agtaaagatc ctatcgtttg gattattttt taagatccta tcatcagtta
     2041 tacttaaaga aagaggaaaa atgtatgaaa atcattcgac aaattgaagt gtttgcacca
     2101 gattacttag gaaaaatgga tgtactgatt gccggtggga agattttggc tgtagaagaa
     2161 cagctagaag gcggctacga aggagtggaa gtagaagaac tttcaggtga gggaaaagta
     2221 ctaaccccgg gtttcattga ctgtcatttc catattttag gtggaggagg agaaggcggt
     2281 tatcaaaatc gaacgccaga agtcacattg agccaattga ctacagctgg tgtaacgaca
     2341 gttgtcggtt gtttgggaac agacggagaa ggtagagata tgactgcgtt gatcagcaaa
     2401 gcgaaaggtc tggaagcaga aggaatctcc acttatgtat acgaaggttc gtaccgattg
     2461 ccggtaaaaa cagtaacaga ttctatcatc aaagactttt tgacaattga taaaattatc
     2521 ggaatcggag aaatcgcagt atccgatcac cgttcttctc agccaagttt tgaagagttc
     2581 gctcgtgcag tagcagatgc tcgtgtagga ggcatgttat ccggaaaagc tgggatcatc
     2641 aatgtccact taggcggagg aaaaagaaag ctagagctat tgacacgaat cgtagaagaa
     2701 acagaaattc cgatcacaca atttttgcct actcatgcaa atcgcacacc agaattattt
     2761 gaagcttgcg tggcatttgc caaacgtggt ggcacgattg acttcactgc tagcgaagat
     2821 cctgattttt gggagaagac agatggtgaa gttcgcttca gcaaagcact gaaacgattg
     2881 atcgaggaag atgtttcttt agataatttc acgatgacct cagatggaca aggaagcttg
     2941 ccttattttg atgaaaacaa tcatttccta gggttaggag taggaagtgc caaggcattg
     3001 ctagtaggga tcaaagaagc tgtgcaaaaa gaaagtatcc ctttggaaat cgccttacga
     3061 gctataacca gtaatccagc acgtatattg aaattggata aaaaaggaaa aatcgaaatt
     3121 ggtgctgatg cagatctgtg tatcttggat aaagaaacgc ttgatattga tacagttatc
     3181 gctaaaggag aaataatggt ccaagaaaaa gaagtcaaag tatgggggac tttcgaaaaa
     3241 agtttttaat caacagctct aactatcttt tttagaaaac gagcgttctg tttgtctgta
     3301 tctgttgttg atgttaccct gaaggtagat caataaacaa actaagagga ggaagcacaa
     3361 tggcagattt aaaaggacgt tctaaagata tgaaagataa agtagtcggt gaaggaaaag
     3421 aagcatacgg caaagcaaca gacgacaaga gcaaagaaac agaagggaaa gcacaatcac
     3481 taggtgctga catcaaagaa aaagctcgaa atttaggcga cgaagtcaaa gacaagtttt
     3541 ctcgaaaaga cgaaaaaaaa taaaaaccac ttattactaa ttgaatcaag caaataaaaa
     3601 atccgaacca tatctgcgag acagatatgg ttcggatttt ttatttgatc attatcctgt
     3661 tttttaaact tcgatttttt gaaatttttt attttttagt tcaaagactt catcacagct
     3721 ttttgctact tcaatctcat gagtcacgat gataatacag cgattttgtt catgggcgat
     3781 ttcttggaaa agtttgacga tatcttgtga agttgtttca tctagatttc cagtaggctc
     3841 atccgctacg attagttgat gttcacagca gatagcacga acgatagcga ctcgctgctg
     3901 ctgacctccc gaaagtttgg tcacctgttt ttttgctaat atttcatcaa ttcctacacg
     3961 agccagtcga tctaaagcaa cttgtcgata attctcttgc ttggtcttag tgatttccat
     4021 tgctgctatg acgttgtcta gagccgataa ataaggaagc aagttgtaag cttggaaaat
     4081 aatcgagaca tcatttttac gataacttct taacccgatt ttgttcagtg aacgatcttg
     4141 atagtatacc tttccttctt ttggtgcatc tagtccagaa ataagcgaaa gaaaagtcgt
     4201 ttttccagaa ccacttgctc ctaaaatagc gtacattttt ccagcttcga aattcagttc
     4261 tacatcttta taaagcgcat cttgcggatc ttgataccaa aatcccaatt gttctgtccg
     4321 taacatcaaa ttcctccttt agtttaatag gatttttctt gggtttaatc ttaatatccc
     4381 tatagaagct aaaaagacag aaaacagact gattccaaat gctactccgg ctaatagtcc
     4441 caattgtgca ggtttgacat tgatttccaa ttcgctgact tgatcagaaa cagcaaatgg
     4501 attattcgtg gttccttgtt gaccatcgcg ttgaccgcct tgttcttgtc tgttacctgg
     4561 catttgtcca ccgccaggag cttgctggtt ttgtgtcgtg gtttctgtct gttggtcaag
     4621 tagctgctgt cccacggcat tcgcgacgac gtttccgcta agcgaagcaa ctcctaatgc
     4681 aagaatcata caaatagcca cttcggtaaa caattggaga atgattttac ttctggattc
     4741 tcctaaagat agcaagacgc ctaactcatg ccgacgctcc cgaatggtaa tcataatgat
     4801 cagagtaagt atgataatac cggctacagc cactagtaaa acaacatttt tagaaaaact
     4861 tgccacgttg tttaaaggtt ccaacattga ctgatacatg gaatcatttg attgtaagct
     4921 aaatgtatct gtgtcgatca gcttctcagc ttctttgaga aactcatcca ttttatctgg
     4981 atcagataga gtgtaaatag cagaatctat cgtgttatcg tcagatgtcc catttagttc
     5041 atttgcaaaa gtgtaagaag tgtaaagtgt attcgacgga ttcatgaagt tgaatttcat
     5101 acccatactg ccgcttgttt cgctggattc ataaattcct ttgatcgtca tttcataagt
     5161 ggtatcttca tcttcagtac tcgtgattgt gaaagtatct ccgactgcca ggtcatttgt
     5221 ttctgctaga gtagaatcaa tgactacatt gtttgtgcct ttgtcgctgt catctattcc
     5281 ttcaccatcc gtgatttttg ctgttccatc agaaaaatta ctattttgtg cagttgctga
     5341 cacaccactg atttgaaagt ctgcttgagt catttgcgga ttcccgccca ttcctccagc
     5401 aggttcaccg cctgcatcag tagtttctga actgtcttca cttgaatcag aactagtgat
     5461 ggcagttatt ccatcttttg ccatagctga ggcggaactt tcaaaggaat aactctttac
     5521 attatctaat tcagctattt tttccgcgtc cgttaaggaa actggtgtaa gagaaaaact
     5581 gcctggatct ggacgttctt cagtggtaga accgcttgaa gaagtttgtt cctgtttttt
     5641 aaatgaagct tctcgattgg cacttaaagt cactgttgct ccaacttctt tttgtgcttg
     5701 attcgctgct tcttctgcag cgcttcgaat cgtgaggcca gctagcacaa aaataagaat
     5761 ggctgtgaac acggcaaaca ataacaatga acgtccccat tttacttttg tacttttcca
     5821 tgcacgtttc ataaaattca tattttgcat ccctcctagt agatgcattt gataatagac
     5881 aacctatctt aaagctagct taaatcttat gctgtttgca caccatcaac atgaggttct
     5941 gtatatataa aaagtcacta tagttattgc aaaaaatgtg gtaaaatgaa aaaagtatgt
     6001 ttcaaaccga cgaagaagaa atgaaagagg aatagcaaaa gaaatcctag atgctacaga
     6061 gggattttta cgtgcatagt tgttctaaca attgctgata ctagcgattt agtttatttg
     6121 ttgtataata atggggaaca gctgaatact tatataagta ttctggctat agcgtttcgc
     6181 aaaagatcca ttttgttaat gaagtatatc caaaataaaa tgcccatcca actgtcagaa
     6241 cgcggatgtg ctggtaattt tcctgctgat tctccataag ttgagaaata gtgattttgc
     6301 atatgcttca ttggaatggc tcatatattt agataaagaa gaaaatcgac atctttaaac
     6361 ttggatgtca taagaagagg tgaagtttgt gagtaccatt aaaattatcc cattaggcgg
     6421 cgttcgtgaa aatggaaaaa acatgtatgt cgcagaagtg ggcgaggata tttttatttt
     6481 agattgtggg ctaaaatatc ctgaaaatga gttgttgggg atcgacgtcg taatccctga
     6541 ttttacgtat ttagaagaaa acattgatcg tgtagctggt gtctttttaa cacatggaca
     6601 tgcagatgcg atcggggcac ttccttactt gttgtcaaaa atttctgtac cagtattcgg
     6661 gacagagttg acgatcgagt tagcaaaatt gaatgtcagt cgtaatgaag cagccaaagg
     6721 atttaaggat ttccatgtgg tagatgagca tacagaaata gactttggtg aaacagtagt
     6781 gagctttttc cgtaccactc atacgatacc agattcagtg gggatcaatc tgaaaacaaa
     6841 agaaggtagt atcgtttata cgggagattt taaatttgac caaacggcta tcccaatgta
     6901 tcaaacagat tatgcacgat tggcggaaat cggcaaagaa ggagtcttag ccttactaag
     6961 tgaatcggca aatgcagaaa atcctcagcc tgtagcttct gaagcccaaa tagcagatga
     7021 agtatttgat acgatcaaat attgggaagg gcgtatcatc gtcgcttgtg tagcaagtaa
     7081 tttgcaacga gtgcaacaag tattggatgc agcagaaaaa gcacaacgaa aagttgtctt
     7141 gactggtcaa gatttcgaac gaattattcg cacagcgatg cgtttagaaa aacttcaatt
     7201 accaagtgaa gatctgcttg ttacgttgaa agacatgaaa aactatgatc cagaagaact
     7261 gatcatttta gaaactggcc gaatgggtga acccatcaag tctctgcaaa aaatggcaaa
     7321 cggtacacac cgtaatgtca agattgaaga aggagatttg gtttatatca ctactacgcc
     7381 aagcatcgcg atggaaacca tcgtggcgaa aacggaagat atcatttatc gagctggtgg
     7441 aacagtgaag ttgatctctg agaatatgcg cgtttctggc catgccaatc ctaatgactt
     7501 gcaattaatg atcaatttga tgaaaccaac atattttgtg ccagttcaag gagagtatcg
     7561 tgaattagct gctcatgcag atttggctca tgcagtaggg atgccataca aaaatatcta
     7621 tattactgga cgcggagacg tgctggaata taagaacaag cgtatgagtg ttgcaggaac
     7681 tacaagtgca gaaaatgtaa tgattgatgg attaggcata ggtgatatcg gaaacattgt
     7741 tttacgtgat cgaaaaatct tgtcagaaga cgggattttc gtagcggttg tcactatcag
     7801 tcggcgagaa aagaaaatca tttcaaaacc tcaaattact tcacggggat tcgtttacgt
     7861 aaaagctagc cgtgatctga tgaaagaaag cagcaatatg attgaaacaa tcgttgagaa
     7921 acatttagaa agcaatgatt ttgagtggag caaattaaag caagacatcc gagatcaatt
     7981 aggccgttac ttatttgaac aaacaaaacg ccgcccagtg atcttgccag tcattatgga
     8041 agctactcaa cgtcgaaata aaaaataaac aaaaagagta agatgaacct ctctatagag
     8101 tgctcgtctt gctctttttt tgcgaaaaac aaataatttt tgaatacagc atccctcgct
     8161 ttataataaa cgatagaata aagggagtga atcaatgatg agtcaaacaa aagtcgaacg
     8221 ttatttagaa gaacaaaaga tcagctatca accacttgat tttagtcatg tggtagaaga
     8281 atctttcaca gaagaactac aaaaaagagg gattgatcca gctttgatat gtaaaactct
     8341 cgtagtgaaa ggaaacaaaa ctggggtgat catcgcattg attcctttga acgatcactt
     8401 agattacaaa aaaacgagac aagtgacgaa agatcgtaaa gttggttttc ctggaatgga
     8461 ttttgtatta atgcatacag gatatcccca cggagcaaac acccctattg gaataaaact
     8521 cgcacatcct gattattgtt ttttagcaga cagttcttta aaagaaaaag aagaagtaat
     8581 tgtgtcaagc ggtgaaatcg ggagaagcct tcggataaaa gtagccgatt tagaaaagat
     8641 cgtccatcct atttacgctg atttaattag ttaagggacg agaagagaag ctaagcagag
     8701 cagagacctc aaacttgaga ttatatctgc ttggtacttg gtgcatcctg tattatctgt
     8761 tacaattgct ttgtacatac tgtactatca atccaatgaa aatatattta gcagctatat
     8821 tttttcattg atgtgcaagt gatgaaatcg tttttaataa aaaagataca gagaaaacgc
     8881 gaggtgtaaa atatgaaaat tacgtatcat ggacattcct gtttaacaat tgaaatggaa
     8941 aatggccaac gcgtgatgat tgacccgttt attacaggaa atccattgac agacttaact
     9001 gcagaagaag taaagacaga ttggttgctg attactcatg gtcatagtga ccacatagga
     9061 gatatgattc ctattgcaaa agaaaatcaa gccacaatta tcagtattgt tgagattgct
     9121 gattatgcga acagcagagg agtagattca ttcggtatga atatcggtgg aaaacatgcg
     9181 tttccattcg gcgtggtaaa attcgtacat gcacagcata gttcaagcta tgaagtagac
     9241 ggtatcgttc agtatatggg cgagcctagc ggaattatta ttcaagcaga aggaaaaacg
     9301 atttatcatg caggggatac tgcttatttc agtgatctag gattgcttgc agaagaattc
     9361 gatattgatg tcgctttctt gccgattggc gataactata cgatggggcc agaagatgca
     9421 gcaaaggctg cgcaaacaat tcgtgccaag aaagtagttc caatccatta caatactttc
     9481 cctgtcattg aacaagatcc cgaatctttt gtagagttac tggaagatag tgaaggaaaa
     9541 ataatgagcg tctcggaaac gctggaagtg taggcagaaa atggcgacaa aacatgattt
     9601 gattttagaa tatattgaaa gtttacctgt tggcaatcga atttctgtaa gaagcatcgc
     9661 aaaagaattg aatgtcagtg aaggaacagc gtaccgtgca atcaaagatg cagaaaatgt
     9721 cggactagtt tcaacaattc aacgagtagg gaccatccgt atcgaacgca aattaaaaaa
     9781 acatattgaa cgtttgacat ttggcgaggt cgtcaggatc attgaagggg acgttttagg
     9841 cggggcggca ggattagaca aggtcttgaa taaattcgtc atcggcgcga tgactaaaca
     9901 tgccatggaa cgctatatta cgccaggatc actaatgatc gtcggcaacc gggcggaagt
     9961 ccaaaaatta gcattggaag atggcgccgc agtattgatc acaggaggat tcaatacgac
    10021 tgctgagatt gcaaagctag ctgatgatct tgaaatgccg atcttacgca cctcttatga
    10081 tacatttaca gtcgcaacca tgatcaaccg agcgttaagc gaccaattga tcaaaaaaga
    10141 tatcatgctt gtcagtgata tctatatgcc agctgaaaag actcattatt tgcagcagac
    10201 agatacgatt tctgattatc agcgtttgtc agaagaaacg cagcattcgc gttttccagt
    10261 cgtcaaccgc catcatcgtt tgatggggat tgttacggca aaagatgttt tagggaaatc
    10321 gccaaaccag atcatcgatc gggtgatgac gaaagaacca atcagtgtaa agaaaacaat
    10381 gagtattgcc tctgttagcc atcaaatgat ctgggatggg ctagaagtca tgccggtcgt
    10441 ttccgatgac ttgacattgg aaggcttgat tacccggcaa gatgtaatga aagcgatgca
    10501 gcttgtccag cgacaaccgc agattgcaga tacgatttcg gatcagatat ctggaaatat
    10561 ccatacgatt gatactgacc gtgaaggaaa tcgtttggaa aaccctaagt tcaaattcgt
    10621 agtagcacca caaatggtca atggcgttgg aaccatttct tttggcgtac ttagcgaaat
    10681 catttctgat gtcgcacaaa aaacaatggt gatgaaccaa aaacgaaata tcttgattga
    10741 acaagtgaac ctacattatt tgcggctgat ccaattggaa agcgaattag acattcgtcc
    10801 acgtgtattg gaaatcggca gacgttccgc taaactggat atcgaagtgt ttattgaaaa
    10861 caccatcgtt tccaaagcaa tcgttgtttg tcaagtaatg gaacgttctt agaaagcagg
    10921 agtgaaatga cagtacagca agaaatagta gatacaataa aaaaatacga taaaatcatt
    10981 atccacaggc atttgcgccc tgatcctgat gcattaggat cacaagtagg attagccgaa
    11041 ctattacggg aaagtttccc agaaaaagaa atacgtcaag taggtgaaac gattgaaggg
    11101 cttcgctttt tagcagaaat gcaagaagtt gaagatgtct tctaccaagg agcgctagtc
    11161 atcgtgactg atacagctaa ttcacccaga atcagtgatc aaagatatca gttggcagaa
    11221 aaactgatca aaatcgacca tcatcccaat gatgaccctt atggagatct tgtgtgggta
    11281 aatacaaaag caagtagctg tagtgagatg attgcagaat ttgctttgat gtttcctgaa
    11341 tctttaaaga tgaacgttaa ggctgcacgg ttactttatg caggaatcgt cggagataca
    11401 gggcgctttt tgtatccctc cacgacgtcc cgtacattag agattgcttc gatattgaga
    11461 aattatccat ttgatgcaag tgctttgaac agagagattg aacaaatgcc gatgaaagtc
    11521 gctaaacttt ccggttatct ttatcaaaat atccaggtgg atgaaaatgg tgcaggaaga
    11581 ataatccttc cgcaagaaat tcttgataaa tatggaatcg atgattcaga gacagcttcg
    11641 atcgtttcac ttcctggagt gattgacgaa gtgctggctt gggggatttt tgttcagcag
    11701 ccttcagggt attaccgcgt acgtttacgt tctaaggggc cgatcatcaa tgaattggct
    11761 aaacgccatc atggaggagg ccatccatta gcaagtggtg cgaatgcaaa agatttagca
    11821 gaagtagagg aaatctataa agagatccaa gatatatgcc aatcgtataa gagcagaaaa
    11881 acagaatgat acttcgttcg aaaacgtgcc ttttgtagta aaaatcacaa atgtatcttt
    11941 tcaagatctt ctttttccgt tacaatagtg ctcactaaag attttaaagg agaaagaaga
    12001 tgacattacc aaattgccca gaatgtggct cagagtacac atatgaagat cgaggcttgt
    12061 tgatttgccc agaatgcggg aacgaatgga acaaagaaga acaagaaact gtcatagaag
    12121 acggattagt ggtattagat gcaaatggaa accgtctgtt agagggcgat agcgtgacag
    12181 tgattaaaga cttgaaagta aaaggtgcgt caggtgccat taaacaagga acaaaagtca
    12241 agaacatccg tttagtagaa ggcgatcata atatcgattg caaggtagaa ggatttggac
    12301 cgatgaaact gaaatcggaa tttgtgaaaa agaattaaag aatattcaag gtcttttcac
    12361 attcttttcc tactactaat gataaaataa agtaaacgaa aaggagtgaa acgaaaatgt
    12421 atggttatgg aatgtatggt tttttagatc cgacatttct tttggtcatt cttggattag
    12481 ttatttccgg aattgcgtca gcgtacgtca atagtacttt ccgaaaatac gatcaagtga
    12541 gaagcaaaaa caatgtgact ggtacacaag cagcacaata tatattacaa agtcaacaga
    12601 taaataatgt cggtgttcaa caaattgccg gagatctcac agataactac aattcaggta
    12661 ataagatgtt gagtttgtct caagcaactg cacagtctac ttcagttgct gcgataggtg
    12721 tagcagctca tgaatgcgga catgcggtac aagatgcttc gggttatgtc ccattgaaga
    12781 ttcgtgcagc acttgttcca gcagctaact ttggttcgat gatttcgttc cccttgatta
    12841 tggtaggtgt attgcttagc tggaactcta cgttaatcaa tatcgggatt ttcgcttttt
    12901 ctttagcatt acttttccaa ttggtgacat taccagtcga atttaacgcg tctcggcgag
    12961 caatccaaat tttgagtgaa ggcggactac tgacggaaga agaagtaccg atggcaagac
    13021 acgtattatt tgcagcagca ctgacttatg ttgcagcagc gctttcgaca ttcctgcagc
    13081 tgttgcgctt gatcttattg ttcggcggca accgaagaga ttaaaataaa gaagttgtga
    13141 aatcagcgct ttgatctgag ctaataccga gagttagctg attgaatacc atttatttgg
    13201 aa
//
LOCUS       DACEPD010000075        13171 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.078, whole genome shotgun sequence.
ACCESSION   DACEPD010000075 DACEPD010000000
VERSION     DACEPD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.078"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IW797_002102"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IW797_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ1224875.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IW797_002103"
     CDS             1995..4661
                     /locus_tag="IW797_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1224876.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IW797_002104"
     CDS             5025..5777
                     /locus_tag="IW797_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1224877.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="IW797_002105"
     CDS             complement(5799..6953)
                     /locus_tag="IW797_002105"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ1224878.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="IW797_002106"
     CDS             7138..9579
                     /locus_tag="IW797_002106"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ1224879.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="IW797_002107"
     CDS             complement(9909..10613)
                     /locus_tag="IW797_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224880.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10800..11045)
                     /locus_tag="IW797_002108"
     CDS             complement(10800..11045)
                     /locus_tag="IW797_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224881.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11062..11913)
                     /locus_tag="IW797_002109"
     CDS             complement(11062..11913)
                     /locus_tag="IW797_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ1224882.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12079..13083
                     /locus_tag="IW797_002110"
     CDS             12079..13083
                     /locus_tag="IW797_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ1224883.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4203 a         2340 c         2750 g         3878 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaagaaaaa taaaaaatgg aacggattat gtatatatac ataatccgtt ccattctttc
    10801 tattttttcg gtatcaaggc aaaaacaggt agttcaggtg aataaatgac ttcatgttcg
    10861 tgccagggtt tgaccattag cgaatgatcg aaccgttttt tcagttcttc gatgatttga
    10921 tcatgtgtcc agtttcttgc aggaaaatgt tggattttct ctgcatcgat caaaaagaca
    10981 aaatcggaat gaaaagtctg ggtaacttct tccgagagag aattcaagcg ttcgattgga
    11041 tccataaaat cacctttcga cttaaaattg ggtagtatct gggtcttttg ctttgccgac
    11101 tacaccatct aactgatcga tttgtttcat ttcttcttct gttaattcaa aatcaaataa
    11161 ttcagtattt tctttgattc tgcttggtgt cactgactta ggaagaggaa ggaattcgtg
    11221 ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt cagcgatttg
    11281 tttcatttca ggcacatcga agattttacc agtgcctaat ggactatatg cttcgaggat
    11341 catatcatgt ttttttgcat actcaactac agttgtctgt aattcgcctg gtgcaaggaa
    11401 aacttgattg accatcggca taatttttgc tgtttttgct agtgtatcta gatggtgggg
    11461 taagaaattg ctcacaccga ttgctttgat tttacctgct tcatacagtt cttcaaatgc
    11521 tcgccatgta tctgcatttg cttgttccca attatcacga aaagcaacag ggtttggcca
    11581 gtggatcaaa aacagatcta aataatctgt ttggagcttc ttcaatgatt cttcaaaaga
    11641 agacatcaca agttcatagc tgtggtttgc attccataat tttgttgtca agaatatctc
    11701 ttctctagga attccacttt ccttaatcgc ttggccgacg ctttcttcat tcttgtatcc
    11761 ttgggcagta tcgatatggc gataacctgc agccagcgct tctttaacag aggaaacagc
    11821 gacatcccca tcaggtgttt gccatgtgcc gaatcctact accgggattt cgtaaccatt
    11881 tgatagacga taagtgcttg ttaatgaatt catactttca gctccttttt tattcgttca
    11941 actataagtg tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac tcttgaaatt
    12001 tttgtttaaa agaaaatgat tttgattctg gtatttttga ttttttttcc gtaaactaag
    12061 acagaaggga atgagaaaat ggatatctat ttaaaaaaag caattctgca tattattgac
    12121 agagaaactg gttctccagt cttttcacaa aaagagctgg atctgacaaa agaatatatt
    12181 cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa aactggacaa
    12241 ctagcagaag aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa ttttgtggaa
    12301 gcaagtgaag caatcgttca gcggtggttc gatatctacc aagaaagtga agaagcacct
    12361 agtgcagatg tatttgttgt cttgtatgaa ttagatacag tgatgtactt atctttattg
    12421 aaagtcaatt atcgtgaggc ttacactcac tttgttgaag cagacgaagc agggatcgac
    12481 aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc agatgaagca
    12541 ttcgctgtga atttgactga ttggacctac gaattaattg aaaaaaaata tgagttttct
    12601 ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa gtgttcctgc accttcttta
    12661 gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa atttgagaca
    12721 gaagaatttg atatcatggc tgatttgaaa gaagctgtct atgatacgat agaagaaaaa
    12781 ggtcggttgg atcatgagat gattgcggaa aaagtcttca aggaaaacat cacggcaaaa
    12841 atggctttta aagaagaagt acaagagcaa ggatttgtcc cagaagcacc tcctgtaaaa
    12901 gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa aattcaagct atcaaacggg
    12961 atcgaactga tcgtaccagt agatgtatat cgcaacccag atttgattga atttgtgaat
    13021 aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt gaatcgttta
    13081 taaaaatgac tagaatatta ggtgttcaag taccagtgat cagttaaatg aattgtgtca
    13141 agagaaggat cggaaaagac gaatataaca a
//
LOCUS       DACEPD010000076        13010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.079, whole genome shotgun sequence.
ACCESSION   DACEPD010000076 DACEPD010000000
VERSION     DACEPD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.079"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(125..1234)
                     /gene="rpoD"
                     /locus_tag="IW797_002111"
     CDS             complement(125..1234)
                     /gene="rpoD"
                     /locus_tag="IW797_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ1224884.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1258..3099)
                     /locus_tag="IW797_002112"
     CDS             complement(1258..3099)
                     /locus_tag="IW797_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ1224885.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3281..5674)
                     /locus_tag="IW797_002113"
     CDS             complement(3281..5674)
                     /locus_tag="IW797_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1224886.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5781..6641)
                     /locus_tag="IW797_002114"
     CDS             complement(5781..6641)
                     /locus_tag="IW797_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ1224887.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6700..7221)
                     /locus_tag="IW797_002115"
     CDS             complement(6700..7221)
                     /locus_tag="IW797_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ1224888.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7541..8260
                     /locus_tag="IW797_002116"
     CDS             7541..8260
                     /locus_tag="IW797_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1224889.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            8286..10877
                     /gene="mprF"
                     /locus_tag="IW797_002117"
     CDS             8286..10877
                     /gene="mprF"
                     /locus_tag="IW797_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ1224890.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11065..12252)
                     /gene="tuf"
                     /locus_tag="IW797_002118"
     CDS             complement(11065..12252)
                     /gene="tuf"
                     /locus_tag="IW797_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ1224891.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12620..12976
                     /locus_tag="IW797_002119"
     CDS             12620..12976
                     /locus_tag="IW797_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1224892.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3740 a         2718 c         2312 g         4240 t
ORIGIN      
        1 aaaataatga atgaatttcc ttcctataga aaaaaaagct tgagactgcg tagtctcaag
       61 ctttttttct tttaaaataa accaaacagt tttactgatt tctcttctgg tcaatagtcc
      121 acgcttattc taagaaatct tttaattgtt ttgaacgaga aggatggcgc aattttctta
      181 atgctttagc ctcgatctga cgaatacgtt cccgagtaac accaaatact ttccctactt
      241 cttccaatgt tctcgtccgg ccatcatcta aaccaaaacg taagcgaagg acattctctt
      301 cccgatcagt caatgtatct agtacatctt ccaattgttc ttttagtaac tcataagctg
      361 cgtgttctgc tggacttgtt gcttcctggt cttctatgaa atcacctaag tgagagtcat
      421 cttcttcacc gattggcgtt tcaagggaaa caggttcttg cgcgattttc aagatttctc
      481 ggactttttc tgttggaaga tccatttccg caccgatttc ttctggcgta ggttctcttc
      541 caaggtcttg cagcaattga cgttggatac gtatcaattt attaattgtt tcaaccatat
      601 gaactggaat acggatcgta cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa
      661 tccaccatgt agcataggtt gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg
      721 ccttcatcag tcccatgttt ccttcttgga tcaagtcaag gaactgcatg cctcgtccta
      781 cataacgttt agcgatactt actactaaac gaaggtttgc ttctgccaga cgctgtttcg
      841 cttcttggtc gccttcttcg attttcaatg ctaacgctac ttcttcttcc gcagttaaaa
      901 gggagacacg gccaatttct ttcagataca tacgaaccgg gtcattgatt tttacaccgg
      961 ttggcgcaga aagatcttct gtttttgctt gttcagcttt tttctcagca ctcttcaagc
     1021 tgtgaattga tggatcacca ttttcatcca ccacactgat acctgcgtct tcaatctttt
     1081 ggatcaattt gtccatttca tctgcattca aagtgaatgg tgtagcaagc tgattggata
     1141 aatcatcata tacaacttgt cgtttgggtt tgttttcttt gatgaaagct gctactgctg
     1201 cttcatacgt ttttttatct gttccatttg ccataaaaga aggcccccct tgagtagcta
     1261 tttggcttgt tttaactgtt tcgttaaatt gatgatttca atcgtcaatt caagctcaag
     1321 ctgtttgttt ccagttcgtc tggcttcttg ctgcatttgt ttttttaatg ttatttcatc
     1381 agctaagcca gatttgctga tcacatctag caagtcttgg atttctcgtg gcgaactttc
     1441 ttctgccacc ggtaaatatt cgatttccac caccaaccgc cttaaatttt catcttgcaa
     1501 aaagtctaaa aactctgcaa ggttgaattc ataatgttgt gagacatatg catcgaataa
     1561 aaaataaact tctgcatatg catcatgaat aaactgaaat tctcgttctt ttaaaatatt
     1621 acggacagaa acttctttaa atgcacgata taatagaatc tgctctgcct tttctacttg
     1681 cgtcaaaggt ctctttttcc gtgcagtttc ttgtctcgta acctgagtgt gttgactttc
     1741 ctgaggttct tgacgatttt gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat
     1801 cgtctctctg ccgatctgaa actctgttga cagttgagat agatacatat cttgttcgat
     1861 tggtgaaaca actcggctaa gttcaactag taattcttcg aggtattcga tcttgtcttt
     1921 ttcattatct aaatttttcc cttgttttaa ataacgtgat ttaaaagtaa agactgtttc
     1981 gcgcccatga gccactaatt cttggaaaga tgcttctcca tacttacgga gatattcatc
     2041 aggatcaagc ttttctggaa cgactacgac agaaagtgct aatcgactgt tttgccgaag
     2101 caattcaatc cctcgattgg ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac
     2161 caattctttc gtcagctttt ccatttgttg gatctgttga gctgttagac ttgtccccat
     2221 agaagctaat ccagttttga taccagcctg ccatgcagca atcacatcca taaacccttc
     2281 gaacaacaaa atttctccgt ttttccggat atcgcttctt gctttatcaa gattaaataa
     2341 aacttgtctt ttattgaaca gttctgtttc tggactattc aagtatttgg gctgatcttt
     2401 ttcctgccct ttttcaggtg caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga
     2461 attacgaagt ggaaacatca ctctttgata aaaacgatca gacatacggc catcatcatg
     2521 cgtgacaaac aatccagagc gttctaacaa ttcatttgaa attgattcat tcttgaatac
     2581 ttgctgaaga aaagaacgct cattaggtgc aaaacctatg ttgaattctt caattaattc
     2641 taaagacaac ccacgacttt gcaaatattc taaagctggc tgtccagctt ttgtatgcaa
     2701 tagcatgtga tgatagattt ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc
     2761 agattcagga ttttggacag ctatagtacg ctgacgccat tgatcttcta acggtatctg
     2821 ttcgaagtct gccactttta ccactgcttc tggaaaactt agaccttcta gttcttgaag
     2881 aaaagtaaac acgtttccgc ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc
     2941 tgcaacagaa aaagacggtg tacgttcttc atgaaaagga cacagcccca agtaattttt
     3001 accagacttt ttcaactgga catattgtcc aatcacctca acgatattcg tttgactgcg
     3061 tactgtttcg ataacctctt gaggaatccg ttgagccatt ttttcacccc ctgctctatt
     3121 gatatctttt gggtttaaca gaagatattt caggagaata accggtaaaa aatcgcacta
     3181 actgaaatca gtgcgatttt aaaggacaca attatacatg tttgattgta tcatatttca
     3241 ttgctttttc aagtataaca catttctatc tcattaagca tcaatttgct actcttttca
     3301 gtaactgatt ttgaatatca gccttccagt acaatactcg aagaacaata tttacgagca
     3361 tgaatacaaa gaagtatcct acgctctctc ccaaccctaa gtttacttcc aaaattccat
     3421 ggaacagcat tgcagctaca tatatcccta cgatcgtcgt tactgctagg aagatgcgca
     3481 atgggttgaa gggataacaa gcttttacaa ctgccatgca actgatccca atcagcaagt
     3541 aatacatcaa agtagtagtt tcagcatttc cccatgcgaa tgtttttcca agtagataca
     3601 ccgcaatgat attcactact acaagcaagg catttggtaa agcattaata agtgctgtag
     3661 gcaaataacg gtaagtgatt tttcttttat cttgttcaaa agataaaaag aaggaaggat
     3721 acccttcaat cgctaaatca atcagcgtga tttggatagg gataaacgga aatccaattg
     3781 ctgtaacggc acatataatc gataaaataa atgaataaat cgttttgata aaaaagatgc
     3841 ctgagacttt cgtcacattg ttgaccactc ggcgtccttc aaacaggact tctggcaatg
     3901 tagtaaaatc agagtccaac aataccagat tcgcaatctg acgtgttgct ccatcacctt
     3961 ctgccatagc aatgctgcaa tctgcttctc ttaacgccaa aacatcattc acaccgtcgc
     4021 ctgtcattgc aaccgttctg ccgctttctt tcaattcatt taccagcaat ttcttttgct
     4081 gcggactcac tcgtccaaaa accgtatagc gatgagcagc ttctcgcact tccatttctt
     4141 cagtaatttg agaaagatcg acatacgcat cataattcgg taatcctgca cgtctagcga
     4201 tattggagac agtaaaagga ttgtctccag aaatcacttt cagatccaca ccttcctcat
     4261 gaagataagc tagtgtttct tgagcgtttt ttcggattgg atcatcgatt tcaaacagag
     4321 caaccggctc aacaaatgct ggtttttctt cgttcaattg ctgatttgga gcgatcccca
     4381 acataagtac gcgatagcca ttcgcttggg ctgtttttag actgtccgga agccgatctt
     4441 cttccaccaa acgttcaggc gctcctacgt acacgactcc cagttctgga aattccatcg
     4501 cgccccattt gcgatcagaa gaaaatgcga ctttgtttgt cacatcatac cgattagaaa
     4561 gctcaaaata atctctcata gcccgcatag tgatgttgtt atcactgctt tctgccagat
     4621 acgtccccat aatttctggg atggctttct cgtacagcgg atgtagtgct tctacccgct
     4681 ggatactcat tttcccttca gtaatggtcc ctgttttatc caagcataat gtatcgacat
     4741 gggccagcgt ttcaattgaa tacatgtctt gaacaaggat tcgttttttt gccagtttgg
     4801 ttacccctgt cgttaatgca atacttatta gcaataccaa accctttgga agcatgccta
     4861 acaaagctgc tgcagatgca ataacagaaa ttttgataga agcatcgcgg atgaacagtg
     4921 cttccaaaaa tagtatgatc cccaaaggaa taatcacgaa actagtgaat ttggatactt
     4981 tgcgaatcga atctacaagt tcagaacgaa tagctttgtg agtctttgcc tcgctagtca
     5041 agcggacagc ataattatct gcacctactc ggataacttc agccatgaca gaaccgcttg
     5101 tcaaaaagct tccagaaagc aattcagctc cagtttcttt gacaatcaaa tcagattcgc
     5161 ctgtcaaaag agcttcattt gcttctgccc gtccagatat aacctgtaaa tcggctggta
     5221 tctgttctcc tgctccgatt tttaccaaat ctcctaatac cagctcttta gtagaaatcg
     5281 attgttcttt accgtcccgg atcactagta cttctgattg ggaaacaatc gataatttcc
     5341 gtacaagatt tcttgcatgt atttcttgat aaattccaat aacgatattg acgacaataa
     5401 ttgctaaata cgccatgttg gaatacgcac ctacaaataa cagacaaatc ccaataccaa
     5461 agttcagaaa attgaataac gtcaatatat tatcgctgaa tatttgtttt gtactttttg
     5521 atgcatcttc ctggtagtca ttttgctggc cagaagccat tttctgaact acctcttgct
     5581 gtgtaagtcc attcattttc tttttctcca ttttccgcca actttcaaaa ataatcacgt
     5641 gtttaagtct agcgtgttcc aagattaaaa acaacttgtt tttataaaat ttatagaaaa
     5701 acacacgaat gattttacaa aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg
     5761 tttttattat caatctatct ttacttatat tgttcctcca aacggctcat ccaccaacct
     5821 agtgctgtac cggctataaa aagaacagga caagctagta ccccagcaac tcccaatccc
     5881 gcgtaatcgg taggaatgat cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg
     5941 aatagcttgc tatatgcctt tgagaaaatg taatccatga tttttgacaa tccaagaaca
     6001 caaagaatcc caccaattcc gatcggcaca atcacttcaa gtcgtacttc tttaaatccg
     6061 tcagccatcg ctttatacaa tcccatatac accaaaaaat tagaagggct caatcctggg
     6121 acgatcatac ccaaaccaat caatccgccg gcaatcagcc atgttccaaa attctgatcg
     6181 acttgcccgt caaataattg agcgccgtat aataaaaaca aataagccag aacaaaagta
     6241 acaaccatga tgatcacatc agaagtatct cttccttttt tccctgcttc tttccataac
     6301 gcaggaacag ttccagcgat acatccgaca aaaaaccata atatcgttgt ttcatacgca
     6361 cctaataaaa aactgactaa aaaagaaagt agaaagatgc ctgtcactcc gcctagacca
     6421 acaggcagaa aaaacaaaac attttctttg aaattttttg taaggtgggc taaaaaagaa
     6481 atcagccgct catacatgcc aaagacagct gctaatgcac caccgctgac tcctggcaag
     6541 ataaatcctg aaccgataaa cattcccttc acaaaccgta acatccagtc tttggttgtg
     6601 taccccactt cttgattttc cattttttgc cttttttcca ctatttgcgc actccttctt
     6661 tcatttaatt ttattcatga ttgaacattt cctgctattt tacttaaaga ctttttcata
     6721 ggtctctatt attttttcat tcattttgac aatatcagga taaaacatgt tacctccatt
     6781 atgatagaga tagccgccat tgtaaagcaa ggactggatc cgccaatatc ggtattgttt
     6841 cccttcctca tttcctaaaa gtgggctcag cacatcttta gaatacgctt ctgctaattc
     6901 tactgtgttt tctccgccat gattttttac ataggcaata taatctttcc caaaattata
     6961 tgcttggaca gctgtccata aatcgcatcc ttgtttcttt gcttctacta tcatttccgc
     7021 caaatatttg acaccttgat caatactgtc agctggatta tcaatgatct cagcttctcc
     7081 atgcagactt tccgagctct gcatgagatc tgtccctttt ccttttgatt cggtcaatat
     7141 tatcgccttg atcaaattct cgtagttttc catatgataa ttttttgcag ctgttgttat
     7201 ttctatttga tagctttcca ctcgttttac cgaacgataa gttcttcctc ataaaaacaa
     7261 caccgcacca ataaccgtca gaatgatcat gtaaaaaaga atacggataa tagataatcg
     7321 ttttcgcttc atttcatcac ctagttattt tgacatcttt ttttatctta ctatttcttt
     7381 atcttcttgg ctatttcttc cttgatttct gtgtacgcct taagaaaaaa tgaattttcc
     7441 tgtatttttt gacatcttac gaaaatgatc gttgtttttc agtaaaaaca attgcccaat
     7501 gactgatata atcgttatac tttacacagt aggtgattat gtgaaaaaag aaaagaaata
     7561 tgcaaaaatg ccagatggga gcgaaattta ctatgaaaag agcggtcaag gcttccctct
     7621 ctttttactg catggcaatg acggcagcgg ccgctttttt tcagaacagg tccctgtact
     7681 cgaacgctac tataccgtgt atctagtaga tagccgagga catggtcgtt cgacaaacga
     7741 agcatctatg ttgaattttc agttaatggc cgaggatctg aatacgatca tgttgctgga
     7801 aaaaatcgat caggcagatt ttcttggatt tagtgatgga gcaaatctag cactggtatt
     7861 tgccagcagc ttccctaaaa aagttcatcg attgatttta aattctggaa acacgcttgt
     7921 taaaggcgtt cgtttttctg ccagagtcat cagtaatatt cattatgcat gggtatggtt
     7981 actctcttta tttcgcccaa gtcttcgcaa aaatctttta gttatcaaac ttttgctgca
     8041 tgacatcggg cttacagaaa atgatttaaa aaaaatcaac tcccccactt tgatcatcgt
     8101 gggaaaaaaa gatgtaatca agctaaaaca ttctttgtat atagctaaaa ctataccaaa
     8161 agcttccttt gtgcttgtga aagaacaagg acatgaatta gctagaaaag atcctgaacg
     8221 cttcaatcga gaagttcttc aatttctttc tgaaacataa ctattaggaa tagaaggtga
     8281 tttatttgtt aaaaaatacc atacaatggc tgaaaaacca cctaggactg ttcaaaacaa
     8341 tctttttgat ttctgtcatt gtcattattg tcggtgaatt gatgtccatc ggtaaaactc
     8401 tatctatcca gcaattagga gaaacgtttg ccactatacc tgtatggaaa agcggactaa
     8461 tgctattgat tggtcttatc tctgtgttac ctatggttgg ttatgatatt attttgaatc
     8521 ggatgcttga acaaaagcag aatcctcgtt acctgtttga gacaagctgg ctgatcaata
     8581 cgatcaacaa tattgccggt ttcggcgggt ttgttagtgt ggggcttcgc tctgaactat
     8641 atggacaaaa gaaagagagc agaaacgtga tccaagcatt atcgaaagtt ttcttgtttt
     8701 tgatggctgg actttctatt tacagtctgc tttctttttt acttgtagtc ttcacgccag
     8761 ttgctccttt tttgaagcaa tattggatat ggttgatcgg cggcggtcta tattttcctg
     8821 tcgtcttttt atttactact ttcaaaaaga aaggattcct tggtggtacc tcacttaaaa
     8881 ctcaaggaca gctattgcta gtttcattgt tggaatggac cggtgtcctg tttagtttcc
     8941 tttctgttgg ttatttaatg gaaatccgga ttgatctgtg gcaaacaatt ccattattca
     9001 ttgctgcttc agtgatagga atcgtgtcta tgattccagg agaaattggg agtttcgatg
     9061 taatgatgat tatcgggttg tccgctattg gtgtgccaag agaaatagtc gttgtgtgga
     9121 tcttgctcta tcgcctgttc tactacatca ttccgttttt gattggaatc gtctttttct
     9181 tcaaaaatat cggttccact tttgatcagc gttattctgg tatccctaaa caattagcaa
     9241 cagaaattgc gcataaaatc gtggttgtct tattatactt ttctggaatc atgctagtac
     9301 tttctgccac tatcccgcaa gcttttacag agtttcgctg gcttcatagt ttgaacccat
     9361 tgaaatttca ttttattatc caatttccta gtatactatt gggatttctt ttaatcatta
     9421 tgggcagagg gatcgcagca cgtgtgaaac gagcatattt gcctacaatc ttcttgattg
     9481 ttctagcttt actttatgtt ttattaagtg attttagttt cacaccggtg gttttcttgt
     9541 ctattctgct tctgatcatt cttgcttcta aaaacgaatt atttcgggaa caattgatct
     9601 attcatggga gtggcgtacg atcgatggaa tcattattgg tgcactgtcc ttgctttaca
     9661 tcgttattgg ggtatacaat ctgccggatt tccctcatcg gcgtcaccat tttatttcct
     9721 ttttcttatt tccttctgaa aagatttggt tttcgggatt gttggccatc atcgctgtta
     9781 gtttcatgat cgttctattt gttcatttcc ttcaaggaga aaagaaacag attggcgaag
     9841 cctttaatga agaaaaggcc ctgaagatcc ttacaactta tggaggaaac tccgatagtc
     9901 aattgatctt cttaaaagat aagcggatgt ttgcctatga aaaagacggt gaaccaactg
     9961 ttcttctcca attcgcttgc ttcaataata aatgtattgt aatgggtgat ccttctggta
    10021 agaaagaaga ttttccagaa gcaatcgaag cttttatcga agaaacagat cgactatgct
    10081 atttaccagt tttctacgaa acaagcgaag agattgttat gattttgcat gaattcggct
    10141 acgatttcat taagatgggg gaagaagctt atgtagacct gaatagtttt acaacttcag
    10201 ggaaaaaaat gaagggcact cgtgctgtct tgaatcggat cgaacgagaa ggatttacat
    10261 ttgatgtctt acaaccacca ttttctactg agcaaatgag tactttcaaa aatatctctg
    10321 ataattggtt gggttcacga aaggaaaaag ggttttcttt aggctttttc tctgaagatt
    10381 atctacaaag agcgcctatt gcagttgtca aaaataccca agaagaaatt gtcgcttttg
    10441 caacgatcat gcctacttac acgaacaata aagtaggaac gattgatctg atgcgatatg
    10501 attctaaaaa agcaccttct ggcagtatgg atttcttgtt cttgcatttg tttgcctata
    10561 tgaaagaaga acatatccaa tggttcaatt tagggatggc ccctctttca aatgtcggaa
    10621 cttccagaaa gagtttttta caggaaagga ttgcatactt ggtttacgag tttggttctc
    10681 atttttattc tttccatgga ttaagagaat ataaaagcaa gtacgctaca aactgggtct
    10741 cacgttatac tctatattct agagatagtt ggattgcata tgttatgatt gctttgctga
    10801 ttattgataa tgcgccagtt gagcagacac aaggtagaat aagtggtttg aaaaaatgga
    10861 tacgaagaaa gtattaaaaa ctgcataccg atagatgaac actctcatga tgaataaaag
    10921 agactgaagt aaaacgaaag ttcatgcttc tacaatctct tcttatacaa ttgaagaaca
    10981 aagagcggtc caaacagaaa tcctgtttgg accgctcttt tattttaaac atttttcatt
    11041 accaaaatat tcgagtcgcg tttcttatgc ttcgatctct gtaacgatac ctgaacctac
    11101 tgtccgtccg ccttcacgaa ttgagaatgt cgttccgttt tcaacagcga ttggatggat
    11161 caagtcaaca tcaatcgtca cgttgtcacc aggcatgacc atttctgtat cttctggcaa
    11221 tgtgattgtt cctgtaacat cagttgtgcg gaagtagaac tgtggacgat agttgttgaa
    11281 gaatggagta tgacgtccac cttcttcttt tgtcaacacg tacacttctg ctttgaattt
    11341 tgtatgtgga gtaatggaac ctggtttagc aagtacttgt ccacgttcga tatcgtcacg
    11401 ttggatcccc cgtaacaaca cgcctacgtt atccccagct tccccgtaat ctaacgtttt
    11461 acggaacatt tctacacctg ttacaactgc tttttgtgtt tcaggtttga tccctacgat
    11521 ctctacctca tcaccgacac gaacagcacc acgatcaata cgacctgagg caacagttcc
    11581 tcgacctgta attgagaaga cgtcttccac tggcaatagt aatggtttat ctgtatcacg
    11641 ttctggtgtt gggatatatt catctactgt atccatcaat tccataatag cagcttcagc
    11701 atctggatcg ccttgcaatg cttttaatgc ggaacctttg atcacaggag tgtcatcgcc
    11761 tgggaaacca tattcgctca ataactcgcg aacttccatt tctaccaaat cgatcaattc
    11821 ttcatcatcg actaaatcaa ctttgttcaa gaaaacaatc aggtatttta caccaacttg
    11881 gcgtgacaat aaaatgtgtt cacgtgtttg aggcatcgga ccgtcagttg cagaaacaac
    11941 taagattgca ccatccattt gtgctgcccc tgtaatcatg tttttcacat agtccgcgtg
    12001 tcctggtgca tcaatatgcg cgtagtggcg tttttctgtt tcatattcta cgtgtgcggt
    12061 attgatcgta atcccacgtt cacgttcttc tggagctgca tcaatactag catagtcttg
    12121 aggatttgct aggccttttt tgcctaatac tgtagtgatc gcagcagtta aagtagtttt
    12181 cccatggtca acgtgtccaa tcgtaccaat gttaacgtgt ggtttacttc tgtcataatg
    12241 ttcttttgcc ataatttaca aacctcctaa ataatataga tcactgaaaa atttcagctc
    12301 tcaccttttt attatagtct ttttttgact aaatcaaaaa gaatacgtcc gacttacttt
    12361 ttgtaagatt cgaactaagc ttattatacg tcaatattgg tcaaagtcaa ctaagagcac
    12421 tcgtctgtct tatacgcgaa aaaagtacct tttataaata aaaaaatgtg ttttttgaga
    12481 aaaacttcaa gatagtcctt aactttcttt tgaagcaata agaacaaaat gaggaaaagt
    12541 ttaaaattca gctgttgcca acaactttca atagacgaaa tcttctattt cgttaataat
    12601 aaggaaagag gtgaagtaca tgaaaaaatt aattggcgta cttgtcgttt taggcatatt
    12661 tctcggcgga agttttgcag cttatcatta tttttacggc ggcgaagctt attatacaaa
    12721 aatcacgacg aatggcgaaa tgtcagctac ccaagcagat aacggagaga aattcacgac
    12781 ttatacctac aagcaagcgg cttataataa agatggtgaa aaaaaacagg tgacgcttcg
    12841 cgaagaaaga gaacatccat taaagatgaa tgcgtatctt aaactcaaag tcaatcctcg
    12901 taaaggtgta ttaagctggg aagaaataaa ggcttctgaa gtgcctaaaa atgcggcaga
    12961 aaaaatcgat aattagtaaa tggtcttaaa attttattag ataaaaagag
//
LOCUS       DACEPD010000077        12893 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.080, whole genome shotgun sequence.
ACCESSION   DACEPD010000077 DACEPD010000000
VERSION     DACEPD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.080"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IW797_002120"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IW797_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ1224893.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1551..2972)
                     /locus_tag="IW797_002121"
     CDS             complement(1551..2972)
                     /locus_tag="IW797_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1224894.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IW797_002122"
     CDS             complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IW797_002122"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ1224895.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5383..6639)
                     /locus_tag="IW797_002123"
     CDS             complement(5383..6639)
                     /locus_tag="IW797_002123"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ1224896.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7219..8520
                     /locus_tag="IW797_002124"
     CDS             7219..8520
                     /locus_tag="IW797_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ1224897.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8589..9188)
                     /locus_tag="IW797_002125"
     CDS             complement(8589..9188)
                     /locus_tag="IW797_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ1224898.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9442..10323
                     /locus_tag="IW797_002126"
     CDS             9442..10323
                     /locus_tag="IW797_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224899.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10336..11946
                     /locus_tag="IW797_002127"
     CDS             10336..11946
                     /locus_tag="IW797_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ1224900.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWKPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12023..12682)
                     /locus_tag="IW797_002128"
     CDS             complement(12023..12682)
                     /locus_tag="IW797_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ1224901.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
BASE COUNT         3809 a         2608 c         2359 g         4117 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt ttatttttct
     1561 tctaaacttt tttctacctc aatcccccaa gaatctttct tgaagtgatt gatgatcaat
     1621 ggaataacga acatgaccac tgtgatagct gctactaata atgcatcact caatgtcgct
     1681 gtacctaatg tagcaaatac gacaacgatg atagagaaga tcagcatgca gctgactacc
     1741 caagctaatc cgtttccgtt tttaccaatt cggtaaggac gttcgaattc tggatgtttc
     1801 ttacgcattt taagcaatga aacagcaatc aaaatgtaca cgatacaata aataacagtt
     1861 gtagcatttg taagtgtcaa gaatacagca cttacgtcat ccattacacc ataaagcaaa
     1921 gcaaataatg aaataacgat tgattgtgtt agaacgacat ttctagatac gccgtatttg
     1981 ttttctttat ggaatccgaa ttttggtgga aggaatcctt cacgagctac ttggatgatt
     2041 gtttttgatg gtccagtaac ccaagctgat aattgtaaaa gcacaccaat aaataccatg
     2101 aagctgaaga tatttccgat cacttctggt aagcctagga tttgacagtc aatcaagata
     2161 ggctgcgtga tatttgctaa ttccattttt ccatctggta cgacatttgc tacaagcatc
     2221 gcattgatga cattcaataa aactaatccg atcaaagcga taaatacgcc ttttgtataa
     2281 ttttttgttg cgtctttcaa acgaggcatg taaacagaac tcatttcgat cccaacaaaa
     2341 ataaatgtga taccagctaa gtatttcaat gaatcaatat gttccaagtc agggaaagct
     2401 tttgaccatg aaaagtcacc tagatagccg tttgagacta gtccgacttt gaaggctgcc
     2461 aaaacaccta aaacgaacat gatgactact gggatatata cccctagcca tacaccgatg
     2521 ttcccgccga cttttgccat atcgaatttc aagttcaaga tcgtgatgat ccagtagata
     2581 actaaaatac aacctaaaac gaaccagtga ttattgccaa gttctacatt accaaatgtg
     2641 tttcctaaca atggtcctaa agtagaagcg accattacca tacctgggaa catttgcacc
     2701 cataatagcc atgcaacgac aaatccccat ttgctgccca gtgctgtttt tacccataac
     2761 tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac cggaaaccat tgatatcgga
     2821 ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca atgtccaacc gactgcggct
     2881 aacgtaggaa tactccgaac cgttgcacac agtgccattg tcagaccgat aaaggtccca
     2941 agacccattt tacttacttc agatttactc accgttgaca ctctccttga ttttttggat
     3001 aaaataacgg tgtctttgaa gtagtttcag caataaacga aacaattcag cgaatatttg
     3061 aggggattct ttgagtttat tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg
     3121 gatcgaagag cttaggtctc cccttgctct tcagatccaa acatgcgtca gaaacagtta
     3181 gtattcaagc tgttttctaa catgattacc ttgcttattt tgcttcgctt gccaataatt
     3241 ggtctgcgta gattttttcc aatttttctt gaagagctgc tttgattttt tcagcatatt
     3301 cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac gctggcacgc aatacagtta
     3361 cttttccggc acggttccat tcttcatctg agaatcctag ttggttcaca aattgtaatg
     3421 ggctgttgcc atagtctggg atagcaaagt cagtatgtga agttaagaac tcattaccgt
     3481 aaatgcttcc tttcacgtat gaagaataat catatacgtc atggttcaat ttattcattg
     3541 caactaagtc atcgttgcct ttttctttga atacatagtc taccatattg aaatctggat
     3601 aagttaatgg atgaacttcg atttctttgt ctcctacttt aaatgacaag tcattcaaga
     3661 agttgtagaa acgatgtgca ccttcgattg atgcacccat taatttacca tatcctgtga
     3721 cattcaaagg taagacatgg tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg
     3781 aaccttccaa gatataagcc ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat
     3841 atgtcgcaaa gtaagagata acgtctctca tgcggatatc tttgatgacg ataccaccgg
     3901 cagaatatgg aacatagccc attttatgtg ggtcaatcgt tacagattct gcttcttcga
     3961 ttgctttata tgcactgtgt acttcttcta agatataatc tttgttttct gtaaagacat
     4021 tgtatttgta atgaacatct tttaaatctt caaatggaat gaagttgttg tcttcgtcta
     4081 agaaaatcgc acggccgtat ccgccataag cagcatctac gtgtaagtag aagtaaatac
     4141 cgtctttttc taatacgcga cgcaatgcaa cgattttatc gataccgtcg attgctcctt
     4201 cttctgttga tcctacaaca ccgactacac ctaggattgg tgttttttca gctgctagac
     4261 cacgaacgat tttttcaagt tcgttgatgt ccatgcgata gttgtggtca actggcactg
     4321 gaatcacttg gtctaaaccg ataccgatga tgtcggcagc ttttagccat gaatagtgtt
     4381 tagtttgtgg aaccaaccat tttcctaatt tttctaaatg ttttccgcta cgtgctgagt
     4441 gagctttgat ttcgtcgatt ttttctggga cgctgtctaa cagattcatg atttcttctg
     4501 ttgacaagtt cattaattcc caatcgcttt ttccagcaac tagttcaggt gtcacttctt
     4561 tcattgcaag cggcaatgat ttgatgttgc gggcatacca aagtccttct aagtttgcta
     4621 aagaaccatc agcaacgatg tggccccagc catctttata gctcattaat tttgcaaatt
     4681 ccattcctac ttcttcttcc atttggcttg ttgcaggaga agattcatag gcaacgttat
     4741 taccattcca tagcatagca aaattgtacg ctagtaaaga tggcatcaat gtttcagagt
     4801 tcatatgacc ccagtaacgt ccggcattat gccaaggaac ggaatgggta cgcatccgcg
     4861 ctgaaatttc tgatagaaca tcctttgttt tattcacagt gtgttcaaaa ctagcactgc
     4921 ttttttcttc tggcgtaata ataggcatgt cttgaggcat gtagttttga cgccagccta
     4981 aatgttcatc caccaattca ttcaacaaag ctttatagat ttggccgttc tcagctttgt
     5041 ccccaatgaa tagggcattt aggtttagat ctttcgacaa tgattcactc atagtgaaac
     5101 cctcctaaaa aaatatttaa attttgtcga gccctacatt acacctgttc ttctattctg
     5161 taaactaagt tttggcctga tttttaatca tattttaata tgtatcaatt atccgaattg
     5221 ttcacacttt tgaaataatt aaaaaaaacg agggaattta caaatgtaaa ctccctcgca
     5281 aagattgtat taacgacaac cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt
     5341 gtaatagtat ttcccttttt caaggtaaaa acttactaaa ttctaatcaa ttactttggc
     5401 caaaaagtaa tttttcttac cttttcgaat aaccacaaac ttaccatcga attcatcgga
     5461 tggatttatg acaaaattta aatcggtaac gcggtcaccg ttaatactta tagctccgtt
     5521 tgaaacatct tcacgtgctt gacgtttaga tggttcaatt tttgtggaaa caagcagttc
     5581 cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc attttaccga atccttgcgc
     5641 gatttcttcc gcatttagtt ctttaatatt tccagagaac aatgcttgcg ttattttttg
     5701 cgcttctttc aagtcttctt cactatgcac aaatcttgta acttcttctg ccaaacggcg
     5761 ttgtgcttca cgtttctcag gttctgtctc tacttttttc gctaagtctt cgatttcttc
     5821 ttgagacaag aaagtaaaga atttcaagta tttcactaca tcgcgatcat cttgattcaa
     5881 ccagaattgg tagaactcaa atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc
     5941 tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa
     6001 cgcttctgct tctggtcctt ctaattttcg gattaggtct aatccagctg tgatatttcc
     6061 ccattggtct gctccaccga tttgcaattg cacatcgtat gttttatgca gatgcaaaaa
     6121 gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat gagattccta cttccaaacg
     6181 gctggcaacg atatcttttg ctaacatcgt gttgatgttg aagtttttac cgtagtctct
     6241 caaaaattca agtaaagaga tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt
     6301 tgcatcttta ccaaacaatt ttctcatttg attggaaagt gcgtccacgt tatgttgcac
     6361 ttgttccatc gtttgcaaca cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc
     6421 tgttccgccg ccaatcagaa tatatggatg atggcctgcc aattggaatc ttttcatcat
     6481 cataaatgga atcaaatgtc cgatatgcat cgaatcacca gttggatcaa cgccgcaata
     6541 aagtgaaatg ctcttttctt caacgagctc gcgaagtctt tcttcgtttg tttgctgatt
     6601 aatggcgtcg cgccaagtta attcatcaat aatattcatt ttactgacct ccgtgtagtt
     6661 tttttatgaa agcaggaacc tgctagcatg tgtaattaat tatttcttca ggataggata
     6721 ggaaataaaa aaagtccctt ggcatataag ccaagggacg aaatctcgcg gtaccaccca
     6781 atttgtatgt ctagatatcc aacatacctc tcgaacattt aacgctgtcg cgtggcaatc
     6841 actgccaaag actcctgaaa tgtaattcgc tttaaagcat gtactggttt gcagcgacca
     6901 ccagctttct gaaacattgg ctttaacaac tacttcgttt cgatcttcgt cttgttatta
     6961 ttaatatgtg tctatcatag agcgcaactt tttctttgtc aacagaaaaa ggatattttt
     7021 tttaaattcc tcattttttc ttttttgaat ccgctctaat tccgaacatt ttattttcaa
     7081 ttattgttaa aagaaataac tttttctgta ttttttctgc aatttatcga gaagtcactt
     7141 tactttgcct cttattcttt cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa
     7201 aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta ttttaggagc cacttctgct
     7261 ttaactatcg ttattttaat tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa
     7321 gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa aagaaccgac agatgataag
     7381 caacaactag ttactcaagc aaaaaatctg gctcaaagct acgattatga ccaagcgatt
     7441 gcgttattgg aaaaagatag taagaacgga tctactgaaa cacaacagct tcttcaaaca
     7501 ctaaaaaagg agaaagaaca actgatcgac tggtctgatc ctactcagat ctctcatgtc
     7561 tttttccaca gcttgatcgt ggacccgcaa aaagccttcc actctcaaca ggcacaagga
     7621 tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa
     7681 aatgattttg ttttagttaa tttgaatgat ttgatccaaa aagacgaaca aggaaatctt
     7741 tcctttaacg ggcttgcgct acctgaagga aaaaaaccat tggttctttc acaagatgat
     7801 gtcagctatt atgaatatat ggataattcc ggttttccta gccgattggt cgtatcaaat
     7861 aacaatgaaa tcaaaaatgt ctataaagag aaagataaag aagaaatcgg tgactttgac
     7921 atggttcctc tgatcgatgc ctttgtcaag aaacatcctg atttttctta tcgaggcgca
     7981 aaaggtacct tggccctgac aggttacaac ggagttttag gttatcgaac atcaaaatct
     8041 caatatggag acaatgaaaa aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa
     8101 cagttgaaga aagacggctg gagctttgct tcccatacat ggggtcactt gaatatgact
     8161 caagcttcac tagaagatgt aaaaaaagac aatgaattgt ggcagcaaga agttgcacct
     8221 atcctaggaa aaacaaatat tctgatttat ccatttggtg cagacatcag cgactggcag
     8281 ccttattcag cagaaaatca aaaatttgct tacttgaaga gtcaaggttt cgatattttc
     8341 tgtaatgtcg atgcttctac accagcttgg ggacaattag gtacggattt ctatcgaaat
     8401 gctcggatca atatcgatgg cattcgtttt caatctgatt tagacggaaa aaatcctatt
     8461 cttgatccat tcatcaatgt ccatgatgtc tatgacaaag aagcacgcga atctgcttga
     8521 ttttacgtca tttatgaacc aaaaaagccg aactgacatt ttatcgtcag tttcggcttt
     8581 tttatgcttt atttcttctt cggtgcgatt cccttgatca ggacttctgt ccattctgct
     8641 gtatatggaa gcagttttgc gtatatctcc ggcttagaaa ttccttctac cattgcttga
     8701 aaatagagga tcaagaactc atccgaatat ttcaaatcca cttttccttc tttccgccct
     8761 cgatggaaaa gatcaagcat gatactgaaa ctttcttgcg tatacgcatc catcgcgtca
     8821 gaaaattctt ttttttcttt ttttgtatag tatgtcatca aatcgatata aaattgtttg
     8881 ttcactcgat tgagataacg gattttgttt tcactcatgg caatcagtgt ttcttcaaat
     8941 ggaagatttt ccgcaatcaa cgctttcgct tcttgcccta aatcattcat aaatgcaaaa
     9001 aatacttcat aaatcagccc ttccttgctg ccaaaatatt taaaaatcgt cgctttactt
     9061 acctgagcgt tctcagctac cttatccatt gtcactccag cggttcctat gtctgtgttc
     9121 atcaagtcaa aagctgcttg taaaatctct ttctttttct cttgtgctct cttttcaaaa
     9181 ccgttcattt gtagagcctc ccgtaatttt aaacttttat aatatatata gttcaaattt
     9241 attctgaggt ttattataac tatctattga gaaagtatca agctagcaat tccttgttta
     9301 tgaattcatt gacagaaatt tataaaaaga atattatgta ctaaaataat aaaaaaagtt
     9361 ttataaaaag gtataaaacc tttttattaa actatttaat cttgtttagt atatattttt
     9421 agaaaaagga gtggggtttt tatggcagaa atcgtcaaag tcacacattt gaaaaagagc
     9481 tttggtcgtt tcgaagcttt aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt
     9541 ggatttatcg gaccaaatgg ttctggaaaa tccacgacga ttcggacact tttagggatt
     9601 ataaaaaaag acggcggaac ggcaacgatt tttgggcaag atgtatggga agatagttta
     9661 tctatccatg aacgtctttc ttatgttcca ggcgatatct ctttgtgggg aaatttgact
     9721 ggaggagcga tcatcgatct cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa
     9781 gatgaactga ttcaacgttt ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa
     9841 ggaaatcgcc aaaaagtggg cttgattgct gctttggctg ttgattcgga tctctattta
     9901 tttgatgagc ctacttctgg tttagatcct ttgatggaaa cgattttcca agaagaagtt
     9961 gaaaaattga aagaacaggg caaatccatc ctactttctt ctcatattct cagtgaagtc
    10021 gagcgactag cggataaagt cgtgatcata cgcgaaggaa cactcgatga agaaggaaca
    10081 ctcgatgaat tgcgtcattt gactcgttcg accatcacta tagaaacaga agcagagaat
    10141 agtcacttag cacaattgaa cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa
    10201 tttgctgctg ataacgaagc tttcgatgtg attttagctg aagcgtccaa attaggtgtg
    10261 aaaaaatttg aatctattcc accgactctt gaggatttat tcatgagaca ttatgaagga
    10321 tgaggaggaa gcgaaatgac tgataaattt tcacgctggt ccaccctttt ttctcaaaac
    10381 cttagccgtg actggaaaaa gatcctgata tggctagtcg gtgtcactgc tttttctggt
    10441 ggttttgtcc ctgcgtttga agaattgact tccggacaag gattagctgg attatacgaa
    10501 accttgcaaa acccggcaat gattgcaatg gtcggaccaa ctccagcttc gaatgctgct
    10561 gagtatactt taggagcgat gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct
    10621 gctgtgatct ctgttttaca cgtggtgagc caaacgagaa aaaaagaaga acttggtctg
    10681 attgaactta ttcgttcctt tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct
    10741 gaaagtattc tgatcaattt gcttttagca atatcgatca gttgtacgat gatttttttc
    10801 caagcggatt cgatcactgc agaaggtgct tggctttttg ggctatctgt aggtagtatg
    10861 gggattttgg ggagtgccgt tgcactgttt gctgcacaac tgatgccaag ttcagccagt
    10921 tcgaatggtc tgtctttagc gtttttaggt ggactttacc ttctacgagc aataacagat
    10981 gtagctgatt cctccctttc gatgtggaaa cctttaggct ggtcttattt gacctatcca
    11041 tttacaacta acaactggat tcctttgctt tatttgtttt tgttcagtct aatcttgata
    11101 atcagttcat ttgttttaga ggaacatcga gatttgaaca ccggctatat ccccgaacgt
    11161 gaaggaaaac cgtatgctaa acgttctctt ttatccttgc gtggtttatt attctatcta
    11221 aataaaggga tgacaatcag ttggcttatc acttttggtt tgatgggggc ggcatacggc
    11281 tctatctacg ggaatatgca acagttttta gaaagtaacg acttaatgaa acagatgttt
    11341 atccaatctg gcgtctcaat tgaaaaaaac tttacaagca cgatcatggt cgtcttgatt
    11401 tgtctagctg ctattttacc aattgctagc atcaatcgtt tatttacaga agaaacccac
    11461 ggacgtatga atcagctgtt cgcaacaaaa atttctagga accaacttta ttggaccaat
    11521 atgttactag ctattctatt tgctatattg gcaatcctga ttagttctgg tgcactaggg
    11581 ctttctgctt tggcttcgat gaaggatgcg aaagacatgg atctattgat gtttcttaaa
    11641 gccgggttca atttcctgcc tgccgtttta gtgtttatcg gaattagtgg ctttctattc
    11701 ggatggcttc ctcgaatcgg taaacttgct tatatctact tagcttactc ctttgcactc
    11761 aattactttg gcggaatttt ggatttacca gaatggtttt ccaaaacagc tgtccctagc
    11821 tgggttccac ggatgccaaa cgattctttt gatctttccg tgttccttac gttatcatgt
    11881 atcagccttg cactgttcgt ccttggttct attggttata ggcgaagaga cttactagaa
    11941 ggataataaa aaagcatgta caatttcatg ctagcctcca aatctgggtc aaaaatccaa
    12001 catttggagg tcattttttt atttacaagt cttctaactg ctctttatca atgatccaga
    12061 cttctcgatt ctttcttttt acataaccat tctcctctaa taatttaaat tttctagata
    12121 aagtttcggg ggtcgtaccg ataaatgcag caagttcttt cattttcatt ggcaggaaaa
    12181 agtgatcctt ccctgtcgct ttagctaagt caagcagata gacagcgact cgttcttcta
    12241 ctttttccat catcaaaaaa gttgtctgct gctgtacttt tgttaatttc tcaacgttga
    12301 ttgctaaaag tttcagacta agggcgggat aatgcagtaa gagttccgag aaatcttttt
    12361 gtctcaatat gcaaatttcc gttttttcta gtgcttcgcc aaataaagac tcatttggaa
    12421 cgcctagcaa ctggctctct ccctcatatc cgcccggctc tattactcgt aaaagctgtt
    12481 cctttccgct agcagataat tgatacactt tcatactgcc ggaggccacg atcactaatt
    12541 gaggttctcc attcggcgca agaatctgtt ctcctttttc cacatagtga tgtttgacaa
    12601 gtgcgtgtac tttttcttga tcttgctgta ctaagtgatt gaacaaagga acaaatgaaa
    12661 cacataaatg atcatgactc ataatctctc ctccttctaa acagctattt ctaataaaga
    12721 aacataactt cccttattcc aactgataat tttcttttat catagcataa acgtcaagcg
    12781 aaccagatac cagcaaatag agtggatttc tttttgtccc ttgctttatt ttccttcttt
    12841 tgagacctta agtaattgat tcctatcaat tttttaaata tttacttttt gaa
//
LOCUS       DACEPD010000078        12670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.081, whole genome shotgun sequence.
ACCESSION   DACEPD010000078 DACEPD010000000
VERSION     DACEPD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.081"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            6..305
                     /locus_tag="IW797_002129"
     CDS             6..305
                     /locus_tag="IW797_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1224902.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IW797_002130"
     CDS             378..1739
                     /locus_tag="IW797_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1224903.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IW797_002131"
     CDS             1761..2021
                     /locus_tag="IW797_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224904.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IW797_002132"
     CDS             2035..2787
                     /locus_tag="IW797_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ1224905.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IW797_002133"
     CDS             complement(2881..3486)
                     /locus_tag="IW797_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1224906.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IW797_002134"
     CDS             complement(3539..4513)
                     /locus_tag="IW797_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ1224907.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IW797_002135"
     CDS             complement(4891..5670)
                     /locus_tag="IW797_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1224908.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IW797_002136"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IW797_002136"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ1224909.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IW797_002137"
     CDS             7161..8558
                     /locus_tag="IW797_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1224910.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IW797_002138"
     CDS             8639..9079
                     /locus_tag="IW797_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1224911.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IW797_002139"
     CDS             9227..9553
                     /locus_tag="IW797_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1224912.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IW797_002140"
     CDS             9554..10303
                     /locus_tag="IW797_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224913.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IW797_002141"
     CDS             complement(10383..10838)
                     /locus_tag="IW797_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ1224914.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IW797_002142"
     CDS             complement(11265..11540)
                     /locus_tag="IW797_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224915.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IW797_002143"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IW797_002143"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ1224916.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4055 a         2176 c         2586 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttgctct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACEPD010000079        12454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.082, whole genome shotgun sequence.
ACCESSION   DACEPD010000079 DACEPD010000000
VERSION     DACEPD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.082"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..358)
                     /locus_tag="IW797_002144"
     CDS             complement(<1..358)
                     /locus_tag="IW797_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1224917.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVSQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVR"
     gene            992..1963
                     /locus_tag="IW797_002145"
     CDS             992..1963
                     /locus_tag="IW797_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ1224918.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(2041..2277)
                     /locus_tag="IW797_002146"
     CDS             complement(2041..2277)
                     /locus_tag="IW797_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1224919.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(2246..2377)
                     /locus_tag="IW797_002147"
     CDS             complement(2246..2377)
                     /locus_tag="IW797_002147"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1224920.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2453..2836)
                     /locus_tag="IW797_002148"
     CDS             complement(2453..2836)
                     /locus_tag="IW797_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ1224921.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2869..3654)
                     /locus_tag="IW797_002149"
     CDS             complement(2869..3654)
                     /locus_tag="IW797_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ1224922.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3832..4932
                     /locus_tag="IW797_002150"
     CDS             3832..4932
                     /locus_tag="IW797_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ1224923.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4922..5746
                     /locus_tag="IW797_002151"
     CDS             4922..5746
                     /locus_tag="IW797_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ1224924.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5752..6558
                     /locus_tag="IW797_002152"
     CDS             5752..6558
                     /locus_tag="IW797_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1224925.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6555..7385
                     /locus_tag="IW797_002153"
     CDS             6555..7385
                     /locus_tag="IW797_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1224926.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            7392..8423
                     /locus_tag="IW797_002154"
     CDS             7392..8423
                     /locus_tag="IW797_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224927.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8530..9803)
                     /locus_tag="IW797_002155"
                     /pseudo
     CDS             complement(8530..9803)
                     /locus_tag="IW797_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            10030..10215
                     /locus_tag="IW797_002156"
     CDS             10030..10215
                     /locus_tag="IW797_002156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224928.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            10339..11055
                     /locus_tag="IW797_002157"
     CDS             10339..11055
                     /locus_tag="IW797_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ1224929.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10957..11477)
                     /locus_tag="IW797_002158"
                     /pseudo
     CDS             complement(10957..11477)
                     /locus_tag="IW797_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            11765..12418
                     /locus_tag="IW797_002159"
     CDS             11765..12418
                     /locus_tag="IW797_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1224930.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT         3625 a         2290 c         2817 g         3722 t
ORIGIN      
        1 ggcgaacata attctttcca aaagttgaga aatgggggat tttctcctcg aaggagtagc
       61 ctaaaaacca gcggtaagcg acattggtat cgacttcctt aatggtttgt cgcatggaac
      121 ggataccgaa taaatactga ataaacacca atttgatcag aatcactgga tcgatacttg
      181 ggcgacctaa atcggagtaa gtggcttcga cgattggata aataaattca aaatcaagcg
      241 cggcatcaat ctttcgaacg agatgatctt gagaaactag atcatcaatc gtaagcatcg
      301 caaattgatt tctaccattc gtattgtttt tagacatcat agctatcact ccttgcattc
      361 gttttcataa atctattata aaagaaaaga ctgtagacat catccataaa atggactttg
      421 tctacagtct ggataacgtg attttttgct gtaatgacaa ggttttagct gtttcagata
      481 ccatgtatcc aaaaatagtt tattgagaaa ataggagagt atttgatact aactagtagg
      541 aaaaaatgag ataaaaagta aatggaatca tttaaggtaa tctgaaataa aaaatataca
      601 gccctgtggt actttaccta caaggctgta tatatttttt actctcagcg tttttgactc
      661 cgtgtctgac tccgttttat tgattttgaa tgttattcaa tgaccattta acaggtcaaa
      721 tttgatgttt ttgattccgt atgacgccgt ttgatatcta gtgatatagt gatttgagtc
      781 tacatcgaat aagggaataa accaataaaa aatgatttag aatattaaaa taacaaaaaa
      841 gaggactacc tgtattagtc ctcttttttg accagatttt gaccagtcta ttttacatcc
      901 tagtatattt atatgagcta attttatgat taaatggtaa gtttttcctt gtgtgtttta
      961 gtttaaacta gtgtgtaatt caaaaatcta tatggaagga acagtattaa gaatagaaca
     1021 gggttctcta catgatggtg ctggtttacg gaccgttgtt tatttaaagg gctgcccatt
     1081 acgctgtgcc tggtgttcga ttcccgaatc acaaagcaag cagattgaaa aaggatttgg
     1141 ccagaccatg accgctgaag aggtaatgga tgagattgag aaggatgctg ttttttactt
     1201 tcactcggat ggcggagtga ccattagcgg aggcgaggca ctggttcagg cggattttgc
     1261 taaagaaatt ttgcaaaaat cgaaatacat tggaatcaat accgtactgg aaaccagctt
     1321 ttgtggtgct tataatgaaa tccaaaaggt agctccctat gtggatacat tgtttgtgga
     1381 tgtaaaaatg tttaccagca agctccacaa acaatggacc ggcttagata accaacaaat
     1441 tttgaagaat attcgtcgct ttttaattga gtatcctaac tgcgaagtgc gaattcgagt
     1501 tcctgtggtt ccaggtatca atatgaacct gaccgagctg ctgacgattg cttgttttgt
     1561 ggcagatctt gatcggttcg taccgttaga attactaccg taccactgct atggtatgca
     1621 cggttaccaa gcattgggac tggagtatcc gttggcagac acgccagcgc cttcacctaa
     1681 agaaatgttt gcattggcag atcaactggc tcgcacggtt cctcatttgc tggtcacagc
     1741 tttaaccaaa acatttattt atttattgaa tccaagcttt atcttatgga taaatgaaag
     1801 acactcacta gctgcagcca gctcaagaat agagcttttg ctgaagtcag tgagtgtctt
     1861 tttacgatgt tttttcgaag ttaatttgtc gaatgaacac tggctcaatc ggtacatctt
     1921 cactgtttgt cggctgagct gcgatcgctg ctacgatttc taacccttgg tatacctgtc
     1981 caaaaacagt ataacgacca tccagctccg gtagtcctcc aagctccaag tatgcttgct
     2041 ttacattagc aggtcagcct gcgcctttgt gttttgcaca ataaaaaact ggttgccgtt
     2101 ggtgtggggg ccgtggtccc ctaaggccaa ggcgccatta aaatgataaa gtccgcgatg
     2161 cacttcatca gcgaaaaagc cctcatagct ggaaacagct tcatcaccag aaccatccaa
     2221 agcgccacct tgaataacaa attccttaat gacacgagcc gaaaggtgtg ttgtcataaa
     2281 cgccttgctc agctagggag cgccagttgg ctacagccag gggagcttgg tcaggaaata
     2341 agcggagacg aatttcacca gcatcggtga ccagcacaca aacttcttcc tgctgcggtt
     2401 tttctaattg aaagagagga aggtttgcaa ctgaaacgcc ttgatcctgc catcaaatat
     2461 cgaaaatccg ccactcgacc ttcgcctgat caccaaggcg agccattccc aataaaacgg
     2521 tataggcggt ttcatccgct tgaaaggtca attgcagctg tcctcgtaaa tcgttgtagt
     2581 catctggggc ggctacttga taaaaaagat gaaactgcgc atgcaaatat tccaaaggct
     2641 gatctttaaa ggctgcaaaa taagcctgaa ctttttcccg ctgcaagctg tcagccacca
     2701 aaaaattttc cagttgttgt tttggaacgg ctgctaacaa ggatcggcaa accgcctcaa
     2761 ttttcaacgc atcccaatgt cgcgcaatta aataatggat gggtgcctct tgattgtagt
     2821 cccacaggtt gctcattttt ttcaccactt tcttctatta tataggaatc acaaaatcgg
     2881 cagcggcgtc aggcggcgtt cttttccttg aatgactagc aacttttcta cctgacagtc
     2941 tgccaacagc tgactcattt ttttaaagcc cagctgataa tcttcggcaa catgagcatc
     3001 cgagcctaag gtaaagagct ttccgccgac actttgatac aaaggaacgg cataacggta
     3061 aaggtcttcg ttgccgtatt tcaagaagct cttggcattt aactccatcg ccaattctcg
     3121 ttcaacaacc tttttgaaaa tcgttagcag ctgagcctca aaatgctccg cgaattcttc
     3181 cgctgtgaag gtgaagcggc gtaatccata atcaaagtga gtcaaaatat ctccttctgg
     3241 aaaatccgtc aagacctgca acatctgatc aaaatattcc ttggtagttt gaatgggatc
     3301 tttggttaaa accacatcgt ccatataatc aaagcggcca ttttgatgaa tacttaccag
     3361 ctttaaatca taaggatgct gagcgaggta atccacgatc cgctcttctt gtcctggaac
     3421 aacgccaatc tcgatacctc gcagaaattt ggtggaatag gtttctttta aagagtccag
     3481 tttgtttata taggctggat aatccggaat gtcatctttt ccggtagatg gattgtttaa
     3541 atcaaagtga tccgtagaaa cgaaaaactc cggctgatag gctaaatagt tttcaaattt
     3601 ttcctctgaa tcaaaggaaa aatacgtgtg gagatgctga tcataatact gcatagggag
     3661 cctccttttt tctactatca taccgcaaaa gctttagaag gttaacggga attcgtcgaa
     3721 atttacaaac catatacaac ctttacattg ttcatgaaac cttttcaaga aactgattct
     3781 ctcattacaa agctttgtta gaatggaatt atcgaaaggg gagaagaatg aatgaaaatg
     3841 aatgaaaaaa ttcgtaacat gactatcggt ttattttgtg cagcaacagt aagtatcctg
     3901 ctgacaggct gcgtctcagg tgaagcaacg aagcaatcgg ctgcggcaga cgactcttta
     3961 gatacgatta tagaaaaagc caaggaagaa ggagagattg ccagcttggg aatgccggat
     4021 acttgggcaa attgggtagg cacttggaac gaccttgaaa aagaatacgg cttaagtcac
     4081 gtggatacgg atatgtccag tgcagaagaa ttggctaagt ttgaatcaga aggaaaaaat
     4141 ggaaccgccg atattggtga tgtcggtatc agcttcggtc cgttggctga aagcaaagag
     4201 ttaaccttac cttataagac caactattgg gaggaaattc cggattgggc aaaggatgat
     4261 gatggggatt ggttgttaag ctatacggga accatttcct ttatcaccga taaagagaat
     4321 gtgaagcaag caccgactag ctggaaggag ctgcaagagg gtgactatca agttagcgtg
     4381 ggggatgtga cgattgccaa ccaagcacaa tttgctgtat tggctgcggc gattgccaac
     4441 ggaggtgacg aaaaggatat tcagccaggt ctgaattact acaaaaaaat ggcagaaaaa
     4501 ggccgtttgt ccaccgttga tgccagcgta gcaaatcttg aaaaaggtga aatcgatgtg
     4561 gcagttgttt gggacttcaa tggactaaat tatcgtgacc aaattgactc cgagcgtttt
     4621 gaggtaactg tgccaacaga cggctccatc attagcgggt atacaacctt aatcaataaa
     4681 aatgcccctc atcccaatgc ggcaaagcta gcacgggaat atattttatc tgatgaaggg
     4741 caaatcaatt tggcaaaggg ctatgcgcga cctattcgca aggtagagct gcctaaagaa
     4801 gtagctgaaa aattactgcc ggaggatgct tatgaagcgg cggtgccagt tgaagacaat
     4861 gaagcttgga acaagacctc tgtagagctt ccagagcttt ggcaggcgga ggtactgacc
     4921 tatgcaaagt agacttgaca aactgattgt agttatacta gacggctgtc gctacgacac
     4981 agccgtcgcc cagcttgggt tcatgaatca tttagttgag caccagcaag caagcttatt
     5041 gaaggtaaaa agtgagctgc ccagcaattc acgtccgtta tacgaagtac tgatgaccgg
     5101 cgttccagcc tatgaaaatg gcatctatag taattaccat gctcagcgat ccaaagagtg
     5161 gtcgctgttt gaattagtta aggaagctgg cggaaaaaca ggagccgccg cttaccactg
     5221 gtacagcgag ttgtacaatc aagcccccta taatcgtttg gttgatcgca tccagcatca
     5281 ggaggagcga ctgattcagc atggcatctt ttatagtgag gacacttacc ctgattcgca
     5341 tttatttgcg gatgcccatt atttagtgaa tcagtatcag ccagactttt tggtcgtaca
     5401 ttcgatgaat attgatgatg tcggtcacaa atttaccgcc gattctcatg aatatgcagc
     5461 agcggttaat caggcggaca gattattggc tatttacctt cctgaatggc tgcaaaaagg
     5521 ttatcaagtc gtggtgactg cggatcacgg gatggatgcg cacggactgc atggcggcag
     5581 cttggctgca catcgtgaag ttccttttta tttgatttca aataaacgcc aaaagctgaa
     5641 ggaacaaact gtaccacagc tgctggcggc tccattgttt tgctggttat taggaattgc
     5701 accgtcagag aaaatgcagg acattcaaga attgatgagg ggatgaaaga gatgaaaaaa
     5761 cgcttggttc tggttcctgc ctcctttatc ttctgtgcat ttttactgct gccgctgctt
     5821 tttatggttc tgtgcagctt gcacagtgat ttaactaacc agtggacatt ggccaattac
     5881 agtgatattt tcagcaacaa atattacaca caggcctttg ttaacagcgt actgattgct
     5941 gtcagctcca gtgtgattgg gttaatcggg acgtttattt tatgtttatg cttaatgaat
     6001 ctatcgacta aattgcagga gaaaatcacc tttgtttcca atttggcagc taattttgct
     6061 gggattccgc tggcattctc ttttattatt ttgttgggga atgtaggtgt gctgaaaatg
     6121 attttgccat ttctagcgga cttcaatttg tattcttggt ggggattgac catcacgtat
     6181 acctatttcc aaattccgtt aggggtcttg tttttgtatc ccagcatgaa ggaagtgaaa
     6241 aaggaatggc tggagtccgc acaattgctg ggcgcttcat cagcgtatgc ttggttcaag
     6301 gtggctttgc cttttttgcg accatcgatt gccagcacct ttgtcatctt atttgctaat
     6361 ggcatgggga cctatgaaac agcgtatgcc ctgaccagca gtaatgtaaa tcttttaacg
     6421 attcgaattg cggctttagt gtcgggagat gtgttcgcca agcctaattt aggcagtgcg
     6481 ttggcagtcg ctttaggtgt cctgctggta acggcaatgt tgattattca aaagaaaggc
     6541 aaggtggtgc agacgtgaaa aagttaataa aaccgatttt gttctgcttt gtcctgtatt
     6601 acttgctgcc gattattgga acgattttgt atgccagctc cactaaatgg tcaaaatcgc
     6661 ttttacctag tgattttacc ttgcaatggt tccaacagct gctgacggac agggaattta
     6721 ttgctgcggt gggtcgctca cttttattag caggtgtggt cttggtgacg attctgttgt
     6781 tgatgattcc caccattatt tggattcatt tgtatttccc tcgtttgaat cgttggctgg
     6841 aaaagctgct cctgctgccg tatgctttac caggagtgat tttggtaacg gctttgcttc
     6901 gaacgtatgc tgaaactggt atcccaatgt ttgtagtgtt ggtaggtgca ttgtttatta
     6961 cggcgttgcc gattgtctat cttagtctta acaaccagat gcgcttaatt aatctgaagg
     7021 aattagtgga tgcagcagaa accttgggcg ccccgatgtc tacgattatt atccaggtgc
     7081 tgtttccaaa catccgtatc ggtgtgaccc tagtttctct gatgattttt tcttcggttt
     7141 ttggggagta catgctgacg aacctgttga ttggcggtcg atttcagacg gtacgcatct
     7201 atatgctgcg gcggatgaac gaaaacggcc atttggctag cgctgtgatg gttttgtatt
     7261 tgctgtttat ggtgggaatt gctttaacta cctttatgct gacttatcga caaaagcaac
     7321 agctgtccct taagcagacg agggaagaga agcagtcgaa aaagaagccc ttaaaggagt
     7381 tgtgacggaa aatgttttta gaaatacatg atctcaatct gttttttgat gaaaaacaga
     7441 ttttagctat tgatgaaatt cagattgaaa agggtgagct ggttacttta ctaggtccca
     7501 gcgggtgcgg aaagtctacg ttgctgcgct gtatcacggg attggaacaa ccaaagagcg
     7561 gtgagattgt gctggacaat gagaatattg ccgataagcc tacgaaggat cgcaatatcg
     7621 gctttgtttt tcaacagtat gcattgtttc caactatgac tgtttttgaa aatgtagcct
     7681 ttggtttgaa ggtaaagaag ctgagtcctg aggtgattca ggaaaaggtt tttgagatgc
     7741 tatcattggt ggatatgacg gagcaggcag ataaaaatgt ccagtttctt tctggcggtc
     7801 aacgacaacg ggtggcactg gctcgctcat tggtcactga accgaaagtc ctgctgctgg
     7861 atgagccatt aagtgctttg gatgcccgaa ttagaaaaca gctgcagcgg gatttgcgcg
     7921 ctatccagca atcgctaggg atgacgatga tttttgtgac tcatgaccaa gaagaggcga
     7981 tgcgaatcag tgatcggatt tttgtgatgg aagctgggcg ggtggctcag gtttccacct
     8041 cgaaggaatt gtatcagaat cctcagagcc gatttgttgc ggaatttatc ggcaactata
     8101 atcgtttcga atggtatgag ctgaatcagt atacccacga gttttccgct aagggcaatc
     8161 gctgcattta ttatttaaga ccggagctga ttcaatttga accggttcct cagggggtta
     8221 agattccggt tctatggaag gaatcattta ttttaggcaa cattcaacgg tatgtttttc
     8281 aaacgctgga gggcaaagaa attttagtgg atcggttgaa cgatcgggaa gagaaaatta
     8341 gcgatgcctt atacattcag ctgaaagata ttctagcaat ccctgtggaa attgatactc
     8401 agcattttgc acaacaaagc tagtgtatag tcggcaaaca tttctggctc tttgtcaaat
     8461 aggactgata gagtgatata agaacatcta aggtagaatt gattttctgc tttagatgtt
     8521 tttttatgtt taataagtga tcagatgatt gattaagaaa attctggttc tcatcctaac
     8581 ccgtaagcta ttcgtttcaa tgctttgatg tgggtatgca gattttctat tttgccattt
     8641 gagtaaggca actctaaggc taaacgaatg ccggattcat agtttataaa cgcttttttt
     8701 atatgatgca gttctcttgg aaccgacttg ggcattgttt ttacaagctg aaagaagatg
     8761 tccgcttctt tgtcataaaa agcttgaaga atttcttgat agtaagcata agctattcgt
     8821 agttcatcag aagtagaaag tagacgttct acgacttcac tttcggttaa ataggcccaa
     8881 ccaaaattac gccattttgt ccatttatca taaacaagcg tgtcctgtcg tttgatcaga
     8941 agtcgagata aagccttcac ttgtcgatag tgtttggctt gtattggatc gtttcggtcg
     9001 aagcttttca tgactcatag tcaaatatcc ttaaaactgc gaatgaggtg cttgacgatg
     9061 tgaaagcgat ccacaacgag tttggcatga ggaaagcact cttttatcag caaagcataa
     9121 gaggcgttca tatctgttac gagtattttt acgtttaaac gtgcacgtcg ttcaaattgc
     9181 ataaaataag aaatgagatc tttcttctta cgtgaaggaa gaatatccag taatttccca
     9241 gtatcgccat ccatacaaga aaaagaatag ggaccgactt gatttttcaa tgaacgaaat
     9301 tcatctacca atgaacagcg aggcaaccaa tttattttca cgtttgtcat ggggagcaga
     9361 gaagcgagtg ttcgcgaaac ggttgaccaa gaaacgccgc aatgtttaga aatcgtcgtc
     9421 attgcttgtt tttcagtcaa ttcctccaat attttataat gtacttgttt ggcgataaaa
     9481 cagttttttt caacaatgta ggtattcgcc gtaaaatagg tgtgacagcc acgacagagg
     9541 taccgttgct ttgccaaagc aagataagtc cgttgattcg cacacttatt gagaaggacc
     9601 agcgattttt tctttccgtt cttaacgatc agcccatgtt gattcacctg tttacactga
     9661 ggacagggct gaatcgtttt aatgagcgtg gcagaaacaa cgatactgtt gattgaatgt
     9721 atttttttct tctcgacatg agtgatttga atgtttccat ctttgattcc tagtaatagt
     9781 ttgataagat gaagatcagg catacagaac gctcctttga ttgtgttgtg gtgattcaat
     9841 tctaacggag cttctgtatg ttttgttttt ttatgctcaa aaggactgat agaatgattt
     9901 actatcagtc ctagaaatta tagcgccaca tttctttcac ctttgacttt tcagacggca
     9961 gtttttatgc tgatactgta gagagagcag gacacagctt ttgcttgttt tgggtaaggg
    10021 ggaaagaaga tgaccaaagc acttattttt gattttgatg ggacgatgat tgacagtgaa
    10081 tggatgtttt acgagagtat tcatcactat ttactgaagc ctatttactg gagcagttcg
    10141 gctataaaat tccttttgca gactatcaac aaggtgttgg gacggcagca gaactgtcct
    10201 tttatgaagg gataagtcaa gcggttggga gaccagtgga tgctgaacat tttgagcagc
    10261 ggatcagcag cactggaaaa tcggagtagt ctcaattctg aactgcggga gctagactat
    10321 ttttttgaag cacatccgat gattaaggaa aaaattgatt cctttgtgac gatcgatgat
    10381 cttcaggtcg gcaaaccaga tccagaaggt tatttgttgt gcttgaaacg gctgagggtt
    10441 gaacaggctg ttgtagttga ggatagtcca gtcggtgccc aagcagcacc gcatatgaca
    10501 gttgatgtac aagcgttgga tgcagatttt ttcgccttta gcgggcataa aatgtgcggg
    10561 ccgacaggaa tcggtgtatt atatggcaaa cgtcaattgt tagaacaaat ggagccagtc
    10621 gaattcggcg gtgaaatgat agattttgtc aatctttacg atagttcatg gaaagagctg
    10681 ccatggaaat ttgaagctgg tactccgaac attgcaggag caatcgatta cttgaatgag
    10741 attggtatgg agaatattca tcgttatgag caagaactgg ttgcctacgt tttacctaaa
    10801 ttacaagcga tcggcggtat caccatttac ggaccgcaag atcctgaaca tcatactggc
    10861 gtggttgctt ttaatttgga tggcatccat ccccgtgatg tggcgactgc tctggatatg
    10921 gaaggtgtcg ctgtacgttc aggacaccat tgtgcacaac ggttcatcga cgatttctgt
    10981 ttgactaatt ggtcgtttaa acagtcgatg ataatgccgt ttgagcggct ccaatgtcgt
    11041 ggaatctttg agtaagagtt tccaatatcg cttgagccga cggtacttct tctgattttc
    11101 agattacgtg attgattttc gtacagttct atgagaatcg cctgcttcag ttcctttgtt
    11161 agttgatgat gttcatcaac taagtccgtt ttcgcagaaa aggtctgccc acaatcatag
    11221 catagaaaac gggtacgttt taattctaaa atagtcaact tttctttgac tttcaacatc
    11281 tgagtacgtg tcgtatagga accgttgcgg ataatacgaa tcgatccaca gtgttcacaa
    11341 tgggaaggtg agtcatccaa tctcccttta ataaactgcg cttgaacgcc tttatattct
    11401 ttttcaatca gccattcttc atcaaaaaat aaatgtttat ctgttaatcc gagtaatttt
    11461 ctagtatgat tatccataga gcgactcctt tggtagggtg atgttttggt cgactaaatt
    11521 ttaccaaaag tgagtctctt tttgagttga aacgaagaaa aagtgttagt agaaaatcgc
    11581 cttcgatttt ccaacaacac aaaatattat agaagccttt tttctaaagg atttcaaggt
    11641 gtctgtttgc atttattaaa aaaaagaaaa agtattggca acagaaggcg tcaccaacac
    11701 tagatattat agagccataa tatgctgtat aatatctagt gtataggctt aagtggaggt
    11761 atatatgaaa atattaactg ttgaagatga taatatgata agagagggaa taagtgaata
    11821 tctttcagaa tttggatata ctgttattca agctaaagat ggaagagaag ccttgtcaaa
    11881 atttaatagc gatataaatt tggttatctt agacattcag atacctttta taaatggttt
    11941 agaagtgttg aaagaaatta gaaagaaaag caatttacca attctaatct tgactgcatt
    12001 tagtgatgaa gaatataaaa ttgatgcatt tactaattta gtagacggat atgtagaaaa
    12061 gccattttca ttgccggtct taaaagctag aatagattct ttaattaaga agaattatgg
    12121 acatttagag aagtttgaat ataaggatct atcggttaat tttaatagct atacagctaa
    12181 aataaatggt gaagaaatag atgtaaatgc aaaagaacta gaagtattaa aatgtttatt
    12241 ggataataat ggacaagtgt taacaagaat gcaaattatt gattatgtat ggaaagatag
    12301 cgaagaaact ccttatgatc gagtaataga tgtatatata aaagaactta gaaaaaaatt
    12361 acaattagac tgtataacta ctataagaaa tgtaggatat aaattggaga gaaaatgaaa
    12421 aaattaaaga tatttccaaa aatgttcata caaa
//
LOCUS       DACEPD010000080        12023 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.083, whole genome shotgun sequence.
ACCESSION   DACEPD010000080 DACEPD010000000
VERSION     DACEPD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.083"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(63..1268)
                     /locus_tag="IW797_002160"
     CDS             complement(63..1268)
                     /locus_tag="IW797_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ1224931.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="IW797_002161"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="IW797_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="IW797_002162"
     CDS             complement(1447..2832)
                     /locus_tag="IW797_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1224932.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="IW797_002163"
     CDS             complement(2861..3247)
                     /locus_tag="IW797_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1224933.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="IW797_002164"
     CDS             complement(3263..3577)
                     /locus_tag="IW797_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ1224934.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IW797_002165"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IW797_002165"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ1224935.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="IW797_002166"
     CDS             4837..6126
                     /locus_tag="IW797_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ1224936.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(6165..6770)
                     /locus_tag="IW797_002167"
     CDS             complement(6165..6770)
                     /locus_tag="IW797_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ1224937.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(6966..7430)
                     /locus_tag="IW797_002168"
     CDS             complement(6966..7430)
                     /locus_tag="IW797_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1224938.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(7452..7772)
                     /locus_tag="IW797_002169"
     CDS             complement(7452..7772)
                     /locus_tag="IW797_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ1224939.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="IW797_002170"
     CDS             complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="IW797_002170"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ1224940.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            9194..9544
                     /locus_tag="IW797_002171"
     CDS             9194..9544
                     /locus_tag="IW797_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1224941.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            9729..10682
                     /locus_tag="IW797_002172"
     CDS             9729..10682
                     /locus_tag="IW797_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1224942.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            10695..>12023
                     /locus_tag="IW797_002173"
     CDS             10695..>12023
                     /locus_tag="IW797_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224943.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARN"
BASE COUNT         3439 a         2390 c         2216 g         3978 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataat aaagaggttg tgacaaagac ttgtcacaac ctctttattt tggaaatact
     6181 tctccgacta cggcatcctc aggcaatact aagattcctt ttttttctgg agcatttggc
     6241 aaatgcaatt cacgagcaga acaaatcatt ccatagctgg ctacccctcg caaagttcct
     6301 ggccagatca tcattccatc tggcatcatg gctcctggtt ttgcaacgac aactttttgt
     6361 ccagcttcga tatttggtgc accgcaaaca atttgcaata ctgtcccatt gtccacttct
     6421 gtctcagtaa tcgataaatg gtcagagtca ggatgcggga cgcatgtttt tacatacccg
     6481 acaacgatct tcggttcatg atcagcttca agctgttctt cgaatcctgc ttctttaatc
     6541 aatcgattca atttcgctaa ttcttcatct gttaagctta cttggccgtt cccttcaatt
     6601 gttagatgat cggagatatg gaaaaagttc caagcaacaa cttgaccttg ttcatctttg
     6661 atccgagcaa cattccattt acgttcgaca gtgttttctg ttcctttatc gtcggctatg
     6721 ataaccatca aagtgtctcc gacgtgtgct ttgttataag caaaaatcat gttctttcct
     6781 ccattatcct ttgctgagat ttatcttaac aaaaaattgc attcactgca tgagattttt
     6841 ttcatgcttt atttccgaag aagcttcttc tggaacacca tttattcgta acaagggact
     6901 gtgacaagac ttttgtcaca gtccctttac tgtttgatta aaaaggttat acaagttcca
     6961 cttgcttaat tacttggctg ttcttcttcg tcagaaacga tcaagacatc acaaggtgaa
     7021 tgacgtatga cgtagctggc tacacttcct gtcataaagc gttcgactgc atttagtcca
     7081 gactgcccaa ccatgattag atcgattgca tatttttttg gcaatttgac tgccatggat
     7141 tcacgaacag agccgtatgc aataattcct tctatatttg tgtaatcaac agattttcca
     7201 tattctttac attcttcgat catttgcttg gctgtttcag tttgctgatc gactagattt
     7261 tgatcaatag gagcatagcc cataaaatta taaaattgct gatcgattac gtttgctaca
     7321 tatactttcc cattattacg gcgagccact tctaacgctt ttttgaaagc agcaagtgct
     7381 tgttcactac cgtcaatacc tactaaaatg ttcttatatt cttgattcat cttgacccct
     7441 cctaacagat gtcagccttt tattacactt tctaggaacg attcgatttc ctgttttgtt
     7501 ttacgatctt tattcactag acgacctaat tcttttccgt cttggatcac gacaaaactt
     7561 gggataccaa aaatgttcca ttcagcggct acatctatgt acttgtcacg gtctacttca
     7621 ataaattgcc aatcagaaaa gtcattttcg atttcaggca tttgcggttt gataaaacgg
     7681 cagtctccgc accagccggc agtaaagaaa aatacacttt ttccagtttc tacgtagcct
     7741 gctaattctt ctaaactatt tgggataatc attctttttc ctctactttc tgtttatgta
     7801 tttttcaatt aaattataca cctttctttc gaactaacaa acattgatcc tctttttata
     7861 aaaaaaatct gacaaaaggt tgcttttgcc agattttata gtttcactat cgcgttattt
     7921 cccgtatacg atcgtgttca ctgttgattt atctaaacct ttcaatgttt gaatcaacat
     7981 ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat ggatataacg
     8041 tcccactacg ccaatcactg tacttgggat tccttcattt tgtgtatgag cagcacctgc
     8101 atctgtaccg ccttttgaaa caaagtattg ataagggatg ttgtttgttt cagcgatatc
     8161 tagcaagtac tcacgcaaac gaggaagcat gatcaaacct ggatcttgga tacgcatcaa
     8221 tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtgtcat ctgctgcaga
     8281 gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt tagaaccacg
     8341 caagcctact tcttcttgga cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt
     8401 ttgtaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt cccacgattt
     8461 gctgatgata ttcttcccgt tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc
     8521 tcctggaaga acaccgtaac tcattgcctc ttctcttgat tcaaacccag catcgaacaa
     8581 aatatctgtt acttctactg atttttgtcc gcttgttcct cgtaataaat gtgggggaac
     8641 ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa aacgttgagc
     8701 agaaacaacg taagggttcc atccgcctag aggtacgact ttaaaaagcc cgttttcttg
     8761 gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta cacgaggtgc
     8821 atccgcttct tcatggtggc gcagcccaaa aatgccgccc aaaccatcta actgtaactc
     8881 atctaccagc ggttccatat gactttccat ataggcgcga acgtcttgtt caaaaccgct
     8941 tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt ccatatgcta
     9001 ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg ataaaatatg
     9061 attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag cggttattta
     9121 tttcaaagaa aatatgagag tgtggtaaaa taaacacaaa gagtagcaag tgagaagaag
     9181 gaggtatatc cacatgtatg aagaaagcga attaagctat tttaaaggcg ggttagccgt
     9241 tgggatcggt ctaggattat tgagtggggt agcttcaacg atttggtatc atcgcaaaaa
     9301 agtgttatca gcagaccaag tgcttgaaaa tatcaaaaca tcttttttaa aagaaggcgc
     9361 aatcgaaggt gcttggatct cttttgaaaa agaacctatt cggaaattcg ccgttcggtc
     9421 gagtgcttat cggggtgggc tttcccggat agaagatggc gaaactgttt attatgaatt
     9481 tttagcagat gctcatacag gaaccgtatt agacatttca agaaaaaagg gcgcaataga
     9541 ttaatacgtc aaaaaaatga aataacgaat acgcacccac tatctaaaag agaggtatcc
     9601 tatccttctt ttatagtggg tgatttttat aaaaataggg ctatttcctc ttggaatagc
     9661 tagtatttgt agttattata cggtattatt gaaataagat tagttttatt atataaggag
     9721 agtgacaaat ggaatcacgt gaaaaaattg ccgtgctcat tccttgttat aacgaagagg
     9781 cgacaatttc aactgttatt gaagatttta aaagggaatt accagaagca gatatttatg
     9841 tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggc ggagcaatcg
     9901 taaagaaaga accacgtcaa ggaaaaggga atgtaatccg tcaaatgttt tttgatattg
     9961 atgccgatta ttatttgatg gtagatggtg atgatactta tcctgcagaa gctgcacatg
    10021 gtttgcttga gaaattacgt tctggcgaag cagatatggt cattggcgat cgtctatcaa
    10081 atggaaccta ttttgatgaa aataagcgtc ctttccatga ttttgggaat aatctggtta
    10141 aaaatacaat tacttactta tataaaacaa aaattcgcga tgtcatgacc ggctatcgag
    10201 gattcaatcg aatttttgtc aaaagcttcc ctattatgag ttctggtttt caaatcgaaa
    10261 cagaattgac gattcatgct ttagataaaa aattcaagtt cgttgagatt ccaatcgatt
    10321 atcgcgatcg tcctgaaggt agcgaatcaa aattgaatac attctctgac ggattcaaag
    10381 tgatcatgat gatcatcaaa atgtgcaaag attataaacc tttgcttttc tttgggatct
    10441 ggaccatctt cttcttttta tttggattgg tcatggggat tcctgtgatt cgtgaatata
    10501 tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga ttgatgactt
    10561 tagcccttct ttcattagta acagggttga ttttggatac ggtcgtcaca aatgccaaga
    10621 aagaatatga attgaattta taccatgtct accacgaata tcaaaataaa gaaatgaagt
    10681 aggaggcctt ttttatgcat aagaaaaagg agaatctatt tcctactttt tttgcaactt
    10741 tatttgcgtc aggttttgct ttttttactt cttatggtct aggaactttg aatccaacag
    10801 taatcattgg tttagccatg gttttgctac ttgcttttaa gcaatatgga atagagttgt
    10861 tttcaataaa aaacaaagtt gcagctattg cccgattgtt tttttctgca gcttttgctc
    10921 ttacactttc tattggcaga cagatacaat ttcagtatgt ctcagcaaac tatacaaaga
    10981 caacgattgc tgatggaagt attttttact ttcttgttta tttctccatt tttgtgtatt
    11041 cttttttatt tttacaatcg atttatttat caatgccaaa actggaaaca ttttttactg
    11101 ctcaaaacac acaggctttc tcttcaagaa aatatttttt attctttact gtctttttag
    11161 ttctgtgttg gcttccttat ctgttggcat tgtatcctgg agtggttttg cctgattcgt
    11221 taagttcggt cgcccaatct atgggtgatg ttcctataag caatcatcat ccagtcttat
    11281 tcaccttact ggtcaagcta tttgttcatg atttaccaac tgatacgatc aatcacggtg
    11341 tatttttatt ttcgattttt caaacattga taacagcttc agcgataagt tattctcttt
    11401 gctggctgtt aaaaagaaac atccattttt ttccggtact cattagttta tgctattttg
    11461 catttgcacc ggtttttcca atctacgcct tgaacatgca aaaagatact ttgttctcta
    11521 tcgcttgctt tttattttcc ttagcggttt ttgactttat ttttcaacga gaaaagccaa
    11581 cgattctcaa tcagatttac gtaatcatcc ttgctttact gaccacctat ctaagaaata
    11641 atgggattta tgtagtaacc ggcacaggtt tgatttgcgg cttgattatt ttgaaacaaa
    11701 aaaggtatca agcattcttt caagtggtgc tgggttatac agtgattgct ggactactca
    11761 tccacccgtt gatggctcgt tatgctattc ctacagaaac tgctgaacaa ttaggtgttc
    11821 ctttgcagca aatttctaga actattgtta tggatggtga cttgtctaag aaagataaag
    11881 cattcatgaa tcagctactc ccgataaaag attatgaagc gtattctcct agtcttgcag
    11941 actcaatcaa gtggcataaa aattttaaca atgagttttt gaatacacat aaaaaagaat
    12001 ttctatcact atgggcaaga aat
//
LOCUS       DACEPD010000081        11568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.084, whole genome shotgun sequence.
ACCESSION   DACEPD010000081 DACEPD010000000
VERSION     DACEPD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.084"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(324..2333)
                     /gene="metG"
                     /locus_tag="IW797_002174"
     CDS             complement(324..2333)
                     /gene="metG"
                     /locus_tag="IW797_002174"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ1224944.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            2601..2879
                     /locus_tag="IW797_002175"
     CDS             2601..2879
                     /locus_tag="IW797_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224945.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            2976..3797
                     /locus_tag="IW797_002176"
     CDS             2976..3797
                     /locus_tag="IW797_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ1224946.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(4082..5092)
                     /locus_tag="IW797_002177"
     CDS             complement(4082..5092)
                     /locus_tag="IW797_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1224947.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(5104..6057)
                     /locus_tag="IW797_002178"
     CDS             complement(5104..6057)
                     /locus_tag="IW797_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1224948.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            complement(6047..7117)
                     /locus_tag="IW797_002179"
     CDS             complement(6047..7117)
                     /locus_tag="IW797_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1224949.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            complement(7123..8166)
                     /locus_tag="IW797_002180"
     CDS             complement(7123..8166)
                     /locus_tag="IW797_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1224950.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            complement(8167..9108)
                     /locus_tag="IW797_002181"
     CDS             complement(8167..9108)
                     /locus_tag="IW797_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1224951.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            9192..9539
                     /locus_tag="IW797_002182"
     CDS             9192..9539
                     /locus_tag="IW797_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ1224952.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            complement(9609..11270)
                     /locus_tag="IW797_002183"
     CDS             complement(9609..11270)
                     /locus_tag="IW797_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1224953.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
BASE COUNT         3280 a         2336 c         2095 g         3857 t
ORIGIN      
        1 aatatagatg taataaagtc attgcattca aactttatga atgtaaggac aataaaaaag
       61 agtaccagat gaatcaatta aaatctgccc gaccctttat cattatgctt tttttctgtt
      121 tttacgattc ctattagtat tattctaaat taacaaaaat cagcgaagct cgtacttctt
      181 tttctaggat ttattttgga tagttctctg tttctgtatt atatgattgg cttcaagtaa
      241 taagaaggaa tttacgaaaa ttgcttctca aatttcggaa aaagggggta tgacacaact
      301 agtgtcatac cccctttgaa tttctatgcg atttctgagc catttggcat ggattttggt
      361 gcttcgatta cttgtaaaga gccatctggt gcttctgcag aaaggatcat tccttgactg
      421 atttgtccac gcatctttcg tggtttcaga tttgctacaa tgacgacttt tttcccgatc
      481 aattcgcttg gatcaggata aaattcagcg attcctgaca agatttgacg gtcttggctg
      541 tctccagcat ctagtcggaa ttgcagcaac ttatctgccc cttctacttt ttgacagttg
      601 atgacttctg ctacttttag ttctactttt tcaaatacat caaatttgat ttgtttttct
      661 ttcgttgaaa caagttctgt ttcttctgga tcccatttga ctgtatcttc gtttgtttga
      721 gtaccttcag acattttttc ttgaataaaa gcaacttctt cttccatatc taaacgtggg
      781 aagattggtg taccttttgc aactactttt gttcctgaag gaaattcgcc aaagtgtaat
      841 ccttcaagat tcattgtttc agagtccaat cctagttgat tgaagatctt ctctggcgtt
      901 tctgtcatga ttggctgaag taatatcgct gcgatacgca aagattcagc taaatggatc
      961 atgacactat tcaattcagc tttcttttct tcgtcttttg ctaataccca tggttctgtt
     1021 tcatcaatgt atttgtttgc acgagaaatc agcacccaga tttcagccaa tgctgtattg
     1081 aattccattt tttccattgc atcatggtaa cgtccgacaa cgttagcagc cgtcgtagac
     1141 agttcactat caaatggagt gacaagtgag gcatagtcag gaacgagtcc atcacaatat
     1201 ttgttgatca tagcaatcgt tcgattcaat aagttcccta gatcatttgc cagatcataa
     1261 ttgacacggg aaacaaaatc ttctggcgta aagacaccat ctgacccaaa cggaacagca
     1321 cgaagcaaat aataacggag tgcatctaaa ccataacgtt ccacaagcat ttcaggataa
     1381 acgacatttc cttttgattt agacattttt ccgtctttca tcaatagcca gccatgccca
     1441 aatacttttt taggtagagg caaatcaagg gccatcagca tgattggcca atagatcgta
     1501 tgaaagcgaa cgatttcttt accgaccatc tgaacatctg caggccagta tttttcgaat
     1561 agactttcat cttcggagcc atatcctagt gcagtaatat aattggataa tgcatcgatc
     1621 catacataca ccacatgttt cggatcactc ttgactggta ctccccaagt aaaagttgtt
     1681 cgtgaaactg caagatcctc taatcctggt ttgataaagt tgttgatcat ttcgttttta
     1741 cgagattctg gctggatgaa ttcgggatgt tcttcatagt attccaatag acggtctgcg
     1801 tatttgctca tgcggaagaa gtatgattct tcttttacca attctacttc gtgtccactt
     1861 ggcgctttac caccgatcac gttaccttcg tcatctttgt acacttctgc aagttgtgtt
     1921 tctgtaaaga attcttcgtc agaaacagag taccagcctt catattcacc taagtaaata
     1981 tctccctgtt ctaataaacg atcaaatatt ttttgaacag ctgctttatg gtaatcatct
     2041 gtcgtgcgaa tgaatttatc ataactgata tcaagcgttt tccataattt cttgacatca
     2101 gccgccattt tatctacata ttcttgcggt tttacaccta gttcttctgc ttttgtttcg
     2161 attttttgac catgttcatc cactccagtg agataaaaca catcaaagcc catcaaccgt
     2221 ttataacgag caattgcgtc acatgcaatc gtagtatacg agttgccgat atgaagtttg
     2281 ccgcttggat aatagattgg cgtagtgata taaaaagttt ccttttctgc cacggggctc
     2341 ttcctccttt atcgcatgat gcgaattcgt ttccttgcta gtataccata aaaaaagcat
     2401 gctgttagct ttttttcaaa atgaaaaaga ctggataaac gacttctatc tacgttcaat
     2461 ctatccttta aaaggatgaa taggttgaat aaatgcgggt ttttgttttg tttcatatct
     2521 actaaaaaac ggctttttat ttctcccctc acacgtggta taataaagac tatctatctt
     2581 agtaagggac tggaacaatc atgcagaaga aacatctttt ttttacactt agtattgctt
     2641 ttctttcatt agcacattta atattcagct atttttatat tcgtatgtat ggttatttta
     2701 acctccatgg gcatttgaac agttttatga cagcagcttg gattttgcgt tttatcattg
     2761 acgtatatat cgttatttgt ggcttctttg ccattcgtga agagcggtat aaagtattgc
     2821 cgttttatct gcttttcttt ttattcaatc tcatacttcc atttattttt catatttaaa
     2881 tgataaccta gatttttcaa gtaaatcctt ggtttttttt cagagtctgc tacaataagg
     2941 taaaaagtac ttttactgtc aatggaggtg gtcagatgaa ccgttttgct tctaaagaag
     3001 aagaaaaatt atattatgaa cataaagcaa gtgaagaaga atttcttaac tggtatcaaa
     3061 aacaggaacg tccagaatat gacaagccct ctttgactgt cgatatcgta ctgatgtgct
     3121 ataacaaaga agaagatcag ctgaaagttt tgttgatcca aagaaaaggg catccttatc
     3181 gaaattcatg ggcattgcct ggtggctttg tccaaaagga tgagtcaact ggagaaagcg
     3241 tcttaagaga aacaaaggaa gaaactggtg tggttatttc aaaagaaaat attgagcaac
     3301 tgcatacatt cagtacgcct aatcgagatc cgcgaggctg ggtcgtcaca gtcagctact
     3361 tagcctttat tggagaagaa ccgctaatcg ccggagatga tgccaaggaa gtacgctggt
     3421 tcacactaga acgtcatagt aacaagatcc atttatctag cggagaagtg gctatctcgc
     3481 ttgatttagt cactggagag tcttctggca aagatacttt ggcttttgac catagtcaaa
     3541 tcatcttgaa agcattcaac cgtgtagtca ataaaatgga acacgagccg caagttctac
     3601 aagttttagg taaagatttc acgattacgg aagctcgaaa agtatttgct aaattcttag
     3661 ggatcgatta taaaacgatc gatcattcca attttaagaa agctatgctc caatatttcg
     3721 atgagatcgg tgaacgtcct gtaggaatcg gccgtccttc aaaaatctat cagctcaagc
     3781 cggggcacca tgaataaggt tgtgagcaaa agattcagac ctaatcattc aagaaaaaac
     3841 gcaaaaacaa ctctttgtga gttacgtttt ttgtttcaga attttgatgt attcgtaaac
     3901 tttctgaata aaaaattaac aatagcagaa aaagctcgta cgaacttttt ctgctattgt
     3961 tttattggtt tgtcataatt agtgagcttt tcttcaaaag aaaaattcct agaaacaaaa
     4021 gtcattaaga ctttttatta acaaggctaa ccctaacgct cctatcagtt tcattcattt
     4081 tttatccctt gataaccgaa tgtctgccca ttttttctac tacttggctc tttcttacgg
     4141 aattgctttc ttctgctccg tcggtattga tcaaaagaaa ttttttatta taccattggg
     4201 acttataacg caattttgtt attaatttat gccagtcgtg aatagaaaaa tcagcatgtg
     4261 cctcttcttt tggaaaagat tctacaaata agtagtcata ttgtttgacc aatttcccag
     4321 ataattgttc aagataatct tctcgctggt tcttttgttt cagataaaga tccattactt
     4381 ttctcttttg tttctgataa ttgcttgcct gatctaacct gatctttcta tggtgagcgg
     4441 agcggacttt cacattcaat ttccgctgtg catgttgcag cttttcttct gtctggtgaa
     4501 attttggtaa agttacttct agaggtcgag atgtttggat caatgtcttc ggatgatagg
     4561 cgatcccgat ccatttagct aaagattctt tttttactgg cgaaacatca caaagtagtg
     4621 cgacataaaa ttcttcatta tatcgagcag aaatcgtagc ggagcgaatc gatccttcaa
     4681 ctggccgatg ctgatttatc ttgattagtt ctttttgttt tggtaacttt aagtagccat
     4741 tttccagata gatcgtacgg gtttggttgt tggtcgtata agattgccac atactttttt
     4801 tcgttttaag ctttgggaag ctggcacgtc ccttgaaata attgcgaaaa gcccgatcca
     4861 aattaagctg tgcatttgct aaagcaagac tgtctacttc ttttaagaat gggtattctt
     4921 tttttaatgt agcgggtgtg agcgaaaaat tggctactcc tgtttgttgc caacttttct
     4981 gccgcttttt cagcaaatgg ttataggtaa atcgagcaca tccaaaagta cgaatcaatt
     5041 gctgtttttg ttcttctgtc ggatacatac ggaatttaaa agcttttagc atctatgtcc
     5101 ctcctagtta gcaaccagaa gagattcctt ttgtcgtttt agtttttttg cgtaatatgg
     5161 gacttcctgc tcagttgcat agacaaaatg ttctggtgcg atttcacgaa gttgacgtac
     5221 ttgtccgtca tctggttcag gttgatattt gattcttctg cgttttctct catggtctgg
     5281 atctggtaga ggaatagcag ataacaaact ttctgtataa ggatgaacac cgtgattata
     5341 gatttcatca cttgttccca tctctaataa caagccgtta tgcattacac cgattcggtc
     5401 actaatatgt ttaaccatcg acaaatcatg tgcaataaat agataggtca attgatgttc
     5461 tttttggaga tcttgcaaca aattgaccac ttgggcttgg atcgacacat ctagtgcaga
     5521 aattggttca tcacaaatga taaaacgcgg ttttactgcc aatgctcgtg caataccaat
     5581 acgctggcgt tgcccacctg aaaattcatg aggataacga gtaccgtggc ttggattcaa
     5641 tccaacagtt ctcaatagat catcgacttt ttttgcacgc tcttcagatg atttagctag
     5701 tccattaaca tcaattcctt cggcaatgat atctctgact ttcatacgag gattcaacga
     5761 agcgtatgga tcttgaaaga tcatctgaac atcatgccgg aatttcgtca aacctttttt
     5821 atctttgatc ttcgttatat cttgaccgtc gaataatatc tcaccatcag taggttcatt
     5881 caagcgaatg atcgtacggc cagtagtgga ttttccgctt ccagattctc caactaaacc
     5941 gaatgtttct ccttcataaa tgtgaaagct gatgtcattt acagcatgta cttcattctt
     6001 ttttcctaca ttgaaatact gtttcaatcc tttaacttca agtagtttat ttggcatcag
     6061 caattcctcc ttgaccgtaa gtatgttttt gtttctcttt aaagatagcc cagcgacgtt
     6121 ggatttcttc tggcggtgtc actttgggtg cctgcggagc aagtagccaa gttgctgcct
     6181 tgtgtgtttt tgatagttca aaaaatggtg gttcttgttc tgcatcgatt ttcatcgcga
     6241 attcatttct tggatagaat gcgtctcctt ttggcgggtt caataaatcc ggtggagaac
     6301 caggaatagc atacaatcga tcgttcgcac tatctaatgt cggcattgaa ccaagcagtc
     6361 cccaagtata gggatgttgc gggttgtaga aaatttcttc cgatgttcca acttcaacga
     6421 ttcttcctcc atacatgaca gcgacacgat cagcgacatt cgctactaca ccaaggtcat
     6481 gagtgataaa gataattgat gtatcgattt ttgtctgaat ttctttcagc agctccaaaa
     6541 tctgtgcttg aatagtcaca tcaagcgctg ttgtaggttc atcagcaatc aggatttccg
     6601 gataacatac cagtgcaatc gcgatcacga tccgttgacg ctgacctcct gagaactgat
     6661 gcggataact gttgatacgt ttctcagctt cgggtattcc aactaattta agcaattcta
     6721 atgcagcttt ttgtccctct ttttttgaaa ctttattatg ctttctaagg gactcagcga
     6781 cttgcttccc aatgggcatc gtcggatcta aagaggtcat tggatcttgg aaaatcatcg
     6841 ctatttcttt accacggatt ttttgcatct ctctttctga ttttccaacg atatcgttac
     6901 ctttaaagag gatttgacca gcatctatat tggcgttgct tgacagcaag cgcatgatgc
     6961 ttctggtcgt cactgatttt ccacttccag attccccaac gatcgccaat gtttctcctt
     7021 tattcaaatg aaaattgaca ccgcgaatcg ctcgtacctt tccagcatac gtatcaaaag
     7081 agatttctaa gtctttcact tctaaaattt tcgtcatcgt cttcactctt tcattttagg
     7141 atcaaaggca tcacgcaatc catcagcaag taagttaaag ccaatcatga taaccgataa
     7201 agttactgcc ggaatccata atagataagg cagatacagg aatgtcttgt acccttcgct
     7261 caacatcgtt cctaatgaag ctgacggagg cgtaagacct aaaccgataa agcttaaaaa
     7321 ggcttcgaag aaaatagcag atggaatact aaacatcatt tgaataataa tgacactaga
     7381 gatattcggc aaaatatgtt tgaatgcaat tttcattctt gattcaccta atgtcaatcc
     7441 agctaaaaca tattcttgat cttttaattt cattgtttgt gcacgaacga tacgtgccat
     7501 tggtatccag tttgtgatgg ccatagcagc cacgattgaa aaaattcctg gttcaaaaac
     7561 gaccaacatc aaaatcatga caacgaggtt tggaattcct gataagattt ccaaaaaacg
     7621 ttgcatcacg ttatcgacac gtccgcctaa caaaccagaa accaacccgt aagctacacc
     7681 aatcgttaca tctagtaatg cagcaataaa tgcgatcagc aacgagattc tcattcccat
     7741 aaataagcgg cttaatacat cacgtcccaa accgtctgtt cctaaaaaga aatttacgtt
     7801 tgaagggacg tttgcttgag catatttatc aacaagttca ccaccaacca ttgcttttcc
     7861 attcaatcca tcgatattga ttcccggaat acgaggaggc aaattgatat aagccacgtt
     7921 ttgggcagtt ggatcatgag gagaaacaaa gattgtgatc acagagatca aaatgatgac
     7981 caatagtaaa accattgaaa taacggcagc tttgtttttc tttaaacggc gccatgaatc
     8041 ttggataaaa ttcaaagaag gtgcagtaat ctgttcacgt tcttcttcgg tatttttttg
     8101 taatggaaga aattcatccg cagggatatc tgcaactgtt tcataacgct ttggtatcat
     8161 ttccatttat cctcggctcc cttctgaaac acgaattctt ggatcaacta gaccgtacaa
     8221 tagatccacg atcaaaataa caaaaatcag cataaatgag taaaggatcg ttacagccat
     8281 gatcgtagga taatcatttg ttgtgattga cttaacgaat tgttccccga taccaggaat
     8341 agcaaagata ttttctacca ccagtgatcc ggtcatcaat gctacagcta acggtcctaa
     8401 taaagtcatc aatggaatca aactatttcg aagaccatgt ttaaaggcga tttcccaacg
     8461 gctcaatcct tttgcttttg ctaattccac ataatcacta tgcagtactt ccaccatttc
     8521 cgtacggata aaccgagcag agtcagcaag cggcgacatg gccaacgcaa ttgttggtaa
     8581 gattgtgtac acaaagccat cccattttgc gattggcaaa acttgtaatt tgattgcaaa
     8641 gatatattgt aataaaacag caaatacaaa gtttggaatg gaacgaccta atatcgctac
     8701 gagtgtagaa gatgtatctg cccatgtatt ttgcttcatg gcagagatcg ttcctaaaat
     8761 cgtccctagg actgttccaa agataattgc ctgtaaccct aactgcaagg atggtccgat
     8821 tcttccagct aacaaatgtg cgactggctg gtttttaaat tggaaggaaa ttccaaaatc
     8881 tccttgaagc aaattagaaa gataaatccc atattgtacg ataactggtt tatccaaacc
     8941 gacttgttta tttagcatct cgattgtttc aggactcaaa cgttcctgat tcgtatatgg
     9001 cgtgcccggc agcaattgca tcaagaaaaa ggtgattgtt gcaattaacc aaagcgtgat
     9061 gatcatgaaa aagacacgtt ttaatacata tttgatataa ctattcacta atgtttcccc
     9121 ctagcttttg acttctgaca gatgtttcgc tacattatta gataatgaac caaaaataaa
     9181 ggagcaatca aatgaaaaaa aaatctatcc cctacgcagt agcttttcta ctgattctgg
     9241 ttattttaat aaaaaatctc atcaaccatt cctttacact cattcaatta tctaatgatc
     9301 tattcttatg gtcattgccg tttctgatta tcggcggttt tttatgggtc ttttcttcgg
     9361 gtttcttcga tcattttcag cgatcggtcc atcttgcacg aacaaggaac cgaaagaaaa
     9421 aaccagaatt ttcctcgctc tcgtctgcca gctacggtat gtacagcttt tggctgatca
     9481 ttgccggaat actaattgct ctttctgcta ttttcatgct tttttctttg ttaggataag
     9541 tgaatatgag aacgaggttg cgacatttgc cgcagcctcg ttttttctca tgctcacggt
     9601 attaacgatt attcttcaac gtatgtccat ttgtaatccc atgaagcgcc tgccgcatgg
     9661 tgaacgatac ctttgatttt ttcattcact aggtgacctt ctgctttttg gtacaccgga
     9721 acgacaccca tatcatcaga gatgatcttg attgcttttt ggaaatcatc ccaacgggct
     9781 tgttcatcgc cagcgtctgt tgatgaagct gctttaactg cttcgtcata ttctgcattg
     9841 ctccagcgac cacggttgta tgaattacct gtgacaaaca aatcagtaaa gctgcttggg
     9901 tctgcatagt cagcagccca tccgcctagt actgcgtcaa aatcacccga agttgagcgg
     9961 tcaagacgta cagagaatgg aacaggtgtt ggtttgactg ttacgccatc caagttttct
    10021 tggattgagt tttgaacata ttcgattact tttttcgatg aatcatcatc agaagccatc
    10081 aaatcaaatt ctaaagaatc aacgcccaat tcttttttcg ctttttccca atattctttt
    10141 gctttatctt tgtcataaga aactaatttt ccagcttctt ttgcaaagtc ttcgttcgtt
    10201 gttggacttt ttaccatatc tgatgggatc aatcctgtag agacaactga tccgtcacct
    10261 agtacttgtt ttactaaagc ttcacgatcg atagaataag aaatagcttt tcttaggttc
    10321 acattgttga atggtgaatt cttttcacgt tgattgaatt cgatatagct tgtccgcgct
    10381 tctttaacag aagtataagc tggatcgttt gcgttttgct gtgctaactc accagacaag
    10441 atcacgtcat ctgcttggcc atctttgaat aagttcaatg cagtagaaga ttcttttact
    10501 acactgactt tgatttcaga aagttttact gtatctttgt cccagtaatg atcatttttc
    10561 ttataagtcc attctgtatc tgttcctggt ccatcaaaat cttctaatgt gaatggtcca
    10621 ttataaacag ctttatcact tgcagatgca taggcatccc cattttcttc cactacggat
    10681 tgtttttgtg ggaaaaatga tgggaaagca agcagatatt ggaaatatgg tgtttgtttc
    10741 gtcaatttga tttctaattc atagtcacca actgctttga tccctagttc tgatttatct
    10801 ttctttccag ctgtaatgtc ttctgcattt tcaactggcg catacaggta agcatattca
    10861 gaagctgtag ctgggtctac tgttcgttgc cagccataaa cgtaatccgc tgctgtaaca
    10921 ggatcaccat ttgaccattt tgcgtcttct cgtaatttga ttttgtaagt taatccgtct
    10981 tcactgactt ctgccaactc gctagctcct gccggctgtg gtttgctgtc ttcatcaaga
    11041 cggtaaattc cttcataaac gttattcaat gctgtaaaac tgattgtatc tgttgcaact
    11101 gataagtcag ctgtaggcat ttcttgaggc acgactaagt taaatacgcc tcctccatct
    11161 gctgaaccgc tttttgctga actactgctt gtggctgaac tgccgccata acatccagca
    11221 agtacaaggc cgcataccgc aaccaaacca aatgtaaatt tctttttcat tttacgtacc
    11281 cctttattgg aattttcaga ataattaata ttttctgaag ttgtttaaga gtttaatgaa
    11341 taattattaa aaaatcaaca cttttttttg tctttttcat aaaataatga ttttttcgtg
    11401 aggtttcaag gaatttttca gaaaaaatag ctttgtacaa ggttttccat ggtcttccaa
    11461 aagccattaa ataattttcg ttatattgtc tattattgcc atacaaagat aaaaaagaat
    11521 aaacaaaaag aaactgtgac aaaagtcttg tcacagtccc ttatctga
//
LOCUS       DACEPD010000082        11493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.085, whole genome shotgun sequence.
ACCESSION   DACEPD010000082 DACEPD010000000
VERSION     DACEPD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.085"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(118..432)
                     /locus_tag="IW797_002184"
     CDS             complement(118..432)
                     /locus_tag="IW797_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ1224954.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(446..706)
                     /locus_tag="IW797_002185"
     CDS             complement(446..706)
                     /locus_tag="IW797_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ1224955.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            876..1190
                     /locus_tag="IW797_002186"
     CDS             876..1190
                     /locus_tag="IW797_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ1224956.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            1192..2847
                     /locus_tag="IW797_002187"
     CDS             1192..2847
                     /locus_tag="IW797_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1224957.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            2930..5065
                     /locus_tag="IW797_002188"
     CDS             2930..5065
                     /locus_tag="IW797_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1224958.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            5240..5749
                     /locus_tag="IW797_002189"
     CDS             5240..5749
                     /locus_tag="IW797_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224959.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            5746..7050
                     /locus_tag="IW797_002190"
     CDS             5746..7050
                     /locus_tag="IW797_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1224960.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFI
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            7146..8015
                     /locus_tag="IW797_002191"
     CDS             7146..8015
                     /locus_tag="IW797_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1224961.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(8127..8447)
                     /locus_tag="IW797_002192"
     CDS             complement(8127..8447)
                     /locus_tag="IW797_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ1224962.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            8601..8996
                     /gene="dhaM"
                     /locus_tag="IW797_002193"
     CDS             8601..8996
                     /gene="dhaM"
                     /locus_tag="IW797_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ1224963.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            8998..9981
                     /gene="dhaK"
                     /locus_tag="IW797_002194"
     CDS             8998..9981
                     /gene="dhaK"
                     /locus_tag="IW797_002194"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ1224964.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            10009..10614
                     /gene="dhaL"
                     /locus_tag="IW797_002195"
     CDS             10009..10614
                     /gene="dhaL"
                     /locus_tag="IW797_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ1224965.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            10761..11426
                     /locus_tag="IW797_002196"
     CDS             10761..11426
                     /locus_tag="IW797_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224966.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         3880 a         2043 c         2434 g         3136 t
ORIGIN      
        1 acttgatgca ggacagcgtt ttcacaacct ttgattcacg gttttccaga agtagcttct
       61 tcgaaaaata agccgaaatt tcttgaagct gatcacttct gtcgcaacct ctactgacta
      121 aggcttgata taagcataac cttgggattg cagttcaact aaatcaagga cacctgctgg
      181 tacaacgatt acttgttcag gtaaagaact ttctgaaata tgattagcac gcatagcatt
      241 ggcgcaagcg tgaaaaacga ctccttgcaa atctaagaat tctgcattgg cagagtctag
      301 ataaccggta atcgcgatgc catttacaga tacgacaatc gtcgtttctg gagaagcttt
      361 tatcaggttt tttacgtttt tccctgtttc tgaccatttt tctaattcat caatatggaa
      421 aacgactttc attgaaatca ttcctttact ttttaataat catcttgatc gcttgttcta
      481 gtttagccgc ttgttcttct ggattactat caggtttctc aaagcaattt ttcagattat
      541 ctgcaacgat catgcccatt acgcgatcaa tactagaacg aacagcgctt aattgagtga
      601 tgacatccat acattctttt tcgtcttcga tcattttttg gataccacga atttgtccct
      661 ctgtacgttt caaacggttc aggatatttt tattatcaat ctccataaca cattctcctc
      721 tatttttata attagataat accccttatc ggtattttat gcaaatataa aaagtagtga
      781 aaaaaatgaa gagtcattct ggttgacata ttaataaagc tcctgtttaa tataccccat
      841 gaggtattta taaaaatgta aatgaggtgc agaaaatgaa tttgctattt caatcaataa
      901 aatcgataac agtcccagaa ttaaaggaaa agcttttgga aaatccagcg ttacttgatg
      961 ttagaacacc tgcagaatac cggggcgggc atataaaagg agctaaaaat gttccattac
     1021 aaagtataaa cagatatgat ggagacaaag aaaagaccgt ctatgtgatt tgtcaatcag
     1081 ggatgaggag taaacaagca gctaaagaat taaagaaatc tggttatgat gtagtgaatg
     1141 tacgtggtgg catgaatcaa tggtttgatc gaacagtagg agggaaataa gatgaaaatc
     1201 gttattgtcg gaggtgtagc aggtggaatg tctgctgcta cacggcttcg ccgattaatg
     1261 gaagatgcag aaatcgttgt ttttgaaaaa ggaccgtatg tttcttttgc aaattgcggc
     1321 ttgccttatt atctttcggg agaaatcagt gaacgggaaa atcttcttgt ccaaacgcca
     1381 gaatcattat ccgctcgttt ttgtttagat gtgcgtccaa atcatgaagt gacagccatc
     1441 tttcccgaaa acaaaacggt agaagtcgta catgagggtc aaaaacacat tgaacagtac
     1501 gatgcattgg ttttatctcc tggtgcaaaa ccagttgttc catcgattcc agggataaca
     1561 gaagccgaca atgttttttc tattagaaat gtaccagata tcgataaagt gatgcatgca
     1621 ttagaaaaac agccaaagcg tgccgtgatc gttggtgcag gattcatcgg attggaaatg
     1681 gcagaaaacc taaaaagaag aggtttagaa gtcatggtga tcgaacaagc accacatatt
     1741 cttccaacac tggatgaaga aatggcagct tttatagaaa aagaattgtc tcatcaagga
     1801 gtagaagtga ttacttctca tgctgtcgct ggatttgaag accacgggaa acgattgcga
     1861 ctggatgatg ggcgtaccat ccctgctgat ttagttattt tatccattgg tgttcgtcct
     1921 gataaccagc tagcagtgac cgctggaatc gaattaggca tacgcggggg tatcctagta
     1981 gacgaacgtt atcaaacgaa tattcctgat atttatgcgg tgggggatgc tatcgttgta
     2041 aaacagcaaa tcactggaaa agatgcactt atttctcttg cttcaccagc caatcgtcaa
     2101 ggtagacaag ttgcggacac gatttccgga atttctcgaa gaaatcaagg cggtattgga
     2161 acagcaatta tacgaacgtt tggaatgact gccgcatcca ccggtttaag tgaaagaaca
     2221 gccaaagaaa acgaactgtc ttttgaagtc attcatgtat caggaaaaga tcatgcaagc
     2281 tattatccag aagcaacaga tattttactg aagttgatct tccatccaga gactggcgag
     2341 atttatggtg cacaaggtgt tggggcaaaa ggtgtggata aacggatcga tattttagca
     2401 acagcaatca aagggcattt gacgatcttc gatttaccgg aattagaatt gacgtatgca
     2461 ccgccatttg gctcagccaa agatccagta aacatgctag gatatgcagc aatgaacatt
     2521 gcagaagggc ttagtgaaac cgtacaatgg catgaattgc cgacagaact agcaaaagga
     2581 aaaattttat tagatgtgcg aacagcagaa gaattggaaa agggcaaatt caaggaagcc
     2641 aaacatatcc ctttgaatga acttcgggac cgattaaatg aattagacag ccaacaagaa
     2701 tatatcgtca gctgtcatag cggattacgt agctatctag cggaaagaat cttgaagcag
     2761 tctggctacc acgtaaaaaa ccttgatggt gcattttctt tatatcaaac tgtccgacct
     2821 gaagaactga tatatcctaa caaatgatac tcgttaaaaa gcttgcattc gactcttaaa
     2881 agaggtaaac taatcggttc caccacttta aaaaaagagg agaatgtgta tggtcaacac
     2941 acaaaaacag ttagaagaag catatctgaa gaacgtgtat caaaagctaa ttgataaaaa
     3001 aaacgaactg cgggaatcga tggatacggc aaaaatagaa ggaaaccagt cgttgaaaga
     3061 aatggctggc gagcttcgat tgaattttga cagttactta gataacctgg atactttttc
     3121 gatgattgaa atgaaaaata gagaaatcga tcagatgaat atcaaacaga aaaacgcaga
     3181 aaatcagcta gcaaagatcg aacgtttatt gaaaagtccc tactttggaa aagtcgttgt
     3241 agactttctg gataatgagc caaaagaatc cttttatatc ggtacagcaa atttcacaga
     3301 tgatgcagat gaaaatcttg tttatgattg gcgttcacca attgccgaac ttttttacaa
     3361 taacatgatt ggtccatctt cttatacggt tcgtcaaaat aaaatcgata cggttattaa
     3421 agaaagacga caatttcttt tggaaagaga tcgtttggtc aagttttttg atacgtctgt
     3481 agctattcaa gatgacgtat tattagaagt cttaggccaa gatgagacca atgaaatgaa
     3541 ggctatcact gctacgatcc aatcggaaca aaacacaatc attcgggatg tgaaaagtca
     3601 aaatatcttg gtaaatggcg tagcaggaag tggtaaaacc tctgctatca tgcagcgtat
     3661 tgcgtatctg ctctatttat atcgagatac gatgacatcc gatgacttgc tgattctatc
     3721 acctaatcat cggtttatcg actatatttc gaatgtccta ccatcattag gtgaaagaaa
     3781 tccactgaat ctaaccatca tacagctggt ttcacagctt tcagcagagg aaatagaagg
     3841 tgaagaggct tatttcaaac gtatcacggg agaaaatgta tcggagcaga cagaacgttt
     3901 gcgtagcaaa aagtttatcg ataatctaaa acaatcagat cctttgtttt tagatcatcc
     3961 gaattttatc cgtggactta ctaaaaatgg aaaaacagtc ctttctaaaa aaacgatcga
     4021 aaaaatatat gagaaagttc ctgctcatcc taaattgatc gataggcttc aagcaacgaa
     4081 aaaggcactg atgagcgaat ggaagaatca tcttttgaaa caagcaaaaa gtcctgctgt
     4141 gcagaatcag gtactctcgt tgacagaaga taggcagttg gaattattcg gtaaattgat
     4201 ttctgatgat tcggaacaaa gtattgtagc ttatgcaagg aaacttttac aaaaaaaata
     4261 tcgaaaaata accagacaaa tcgaagaaat ggcatgggtg gatgagcatc agttgtttga
     4321 aagaatttat gaaaaacgat atggctcagc ttatgcttgg caacccacac gtactgttga
     4381 tgaagcagtc attatattag ctattcgtca tttacttgtt gaaaaggtaa atgtaccagc
     4441 atttcgctat ctattgattg atgaagtaca agattatacc ctggcacaat taggtctatt
     4501 gatcgaatta tttccaaaaa ctcactttac tttagtaggt gacgagaatc aggcaatctt
     4561 taattcttcc actacttttg ctgatattat gcgttgtttc gacgactacc atctgccgat
     4621 ccatcggtat gatcttagaa atagttaccg ttctagcgga gcaatcacag agcttttcaa
     4681 aacctatgct gtggatcaag agaagatcga catcgtatcg atccgaccgc aaggaaaaga
     4741 acccgaatac cgctcttttc gatcctctga agaattgttg aaaatactag ctgagatttt
     4801 ggtttctcta aaaggagaaa aagcggcagt tatcacccaa acagaagagg aaacccaaac
     4861 gctacaagaa tcgattaaaa aaaattcata tggattagaa aactgccaaa tcattccttt
     4921 gagtctagca aaaggactgg aatttgatca tgtaatctta tatccatttg aaaacgatgg
     4981 agatgagcaa agaagacgca gacaaatgta cacggcgata tctagaggaa tgaaaagcat
     5041 tgtcgtattg gaaagagcaa cataattaag agataaaaaa taaaaagaac aactgataaa
     5101 aagaacaaaa tatttttcat ttgctgatga gaaatgtttt gttctttttc ttaggaaaaa
     5161 tttaggaaca ctttatgtga aaagaaatga aatgtatggt aaactcctac gtcgtaacaa
     5221 aaaaacgtga ggtgaggata tgaaaaaagt aacaagagaa gaagttgcat caattttggt
     5281 gaaagataag tatttttcaa aaggtaatta ctggttaaag atttggcaaa cgttagtggc
     5341 tcttttagga tggatgatcg ttgttcttcc ttttatatgg gtctttttcc cattaacgag
     5401 accagaaaga gccaaggatt tttccttgta cgctttttta tcagaaaaga aaatgctgta
     5461 tttcttaatc ggcttttttg tattgatttt tttcagtttc ctgattacct catttgtatt
     5521 gactaggtgg aacaatcgaa atctatacaa aaaagtaaat aaatcttttg aatatgaaga
     5581 tgaaaaattg aaaaaaagac aagaattact tgaagaagtg tatacggaac gttttggaac
     5641 aaaagaagag agagaaacgg tccgttttta ttctgtctcc gaagagaaaa atctagatac
     5701 gacattcatt gcagatttgt ataaagaaaa tggggtggaa ctgaaatgat ctcaatcacc
     5761 catacaggta atttattctt agatacttgt ttgtctatta tgtatttttt tctcgtttct
     5821 tatccgattt taggcggatt tgtatggttt attggtgtct ggtgttatgt gtttatatac
     5881 aaacataagc aaaaagaatg ggtagatgtt cccttgagtg tcgaaccgtt catcacgatc
     5941 atggtgccag cccataatga agaaattgtg atcgaatata caatcgagta tttgatgacg
     6001 aaattgaatt atcacaacta tgaggtatta gtgacagacg atggttcaac ggaccaaaca
     6061 ccagaaatat tagctagact gatgaaaaaa tacgctaatc ttcgtgtggt tcggatcgaa
     6121 aaaaataaag gaaaagccca cgcgtttaat atcggacttg cctttgcaaa agggaaattg
     6181 attttaagca atgatgcaga cacggtaccg gagccagatg cactgattag gtatgtgaat
     6241 tatttcattc gccctggtgc aagacatatt gctgcggtca ctgcaaatat ggatgtccaa
     6301 aatcgtacca aattgattgc aaaatctcaa acggttgaat tctcaagtat cgtgggcatc
     6361 attaaacgaa cacagtcagc tgttttcggt ggtctttatg cgtacagtgg agcaaatacg
     6421 atgtatcgaa aagaagcctt gatcgatgtg ggcggattca gacaagatcg agcgacagaa
     6481 gacatcagta ttgcttggga tcatcagtta aatgactggt tatctgtttt tgctcctgag
     6541 attatctttt tcatggaagt tcctgtgacg ttgaagatgc tatatcgaca cagaaaacgt
     6601 tgggcaaaag gtggaacaga agtctggctg acgaatttca aaaaagtgat gctccatcct
     6661 tttaaacata ttggaagaac gatcatcttc attgatcaaa cattgagtat tgtctggtca
     6721 atattctttt gtatttccgt agttcttttt gctggattaa tcgggcatta tgtatatcaa
     6781 ggaaactacg aacagattta tattactttt acattcagtt ttgtgttcat ctgctttgaa
     6841 atggtcgcag gatttttcca gttgctagct tcgctgattg tagatgatcg acgaagaaaa
     6901 ttgaaatacc ttcttttcgc accactatat atgcttttgt tctggatagt gaacgcgata
     6961 acgatcgtga cgactttcat tccagcagtc aaaacgattt taggttatgg aagcgggaca
     7021 tggaaaagcc cggaacgtac aaaaaaataa aagatgcacc gttttttaga caaacagcct
     7081 gttgtgttta tggttcttcc aaacattttt gcttataata agaaattagt taaaaagtag
     7141 taaggatggg aaaagtgatg aaaaaaagaa tgaagccgtt aatctggcca attgctgttt
     7201 ccagtatttt cttattggga cagacagctt ttgcggaaac ttctgaacct gctcaaacag
     7261 aaccttcaac aaccataagc tcttctggtg ctgaaaacgg tgcctcggaa acagaaatga
     7321 cttccaatgt accgacagat aattcaggag aaacgatttc atcttcagaa gatacatcaa
     7381 taaatagttc tacctcttct gaccaaatcg atcatttgca agacttggaa aataaaagaa
     7441 aagaactcga agaagcatta gaagctgcaa aaaacaaatt gaaagaagac caagcagcaa
     7501 tcgatcaaga tttagctgat cttaaaaata aagtgggaaa tgaccgtgca caacttgaga
     7561 aaaagcttgc cgaagcgaaa gcaaaagcgg aagctgtcca aacagatctt gacgggaaga
     7621 tccaacaagc aaaagaattc aatcagcaat taaatcaggt tgctcaaaca gcttctggaa
     7681 agctaagtca aactcaagcg gatatcacag cggccagtca agcaatccac ggaaagatca
     7741 gcgaagcaca aacagttgct aatgattttg aagcaaaaaa agctgactta gcagctagtt
     7801 tccaacgaat aaaagatatt ttgcattcgg caggagaaaa atcagtgacc tctggaagca
     7861 cgacagaaac agttcagcaa acgacagagc catccaaatc aacaagtcag actttgccaa
     7921 aaacaaatga aaaaacagct tctgtcagtt attccttagc aggggcgggt gtgttagtcg
     7981 cagcagctta cggaatcatc cgcaaaaaga aataaaaaag agaggatggg aaggtagagt
     8041 acctcgaagt tagactgaaa aatctaattc ttgagtgcca ctacatgtcc catctctctt
     8101 tagttattaa aacagatgtc ccctacttat ctcgtggaga cagctgttcc actacatgtg
     8161 accatcagca tgccattgtc gctgcctaat acttcatagt ccatttttac gccaatgatc
     8221 gcatcagctc ctaatgcttt tgcacgttcg gccatttcgg caagtgcttc ttctcttgca
     8281 cccattagtt cgttttcata gctttttgag cggccgccaa agatatttcg caaccccgcc
     8341 tccaagtctt taaatgcatg gatcccagta atgacctcac caaaaacgat ccctttataa
     8401 gctactacgg cttttccctc tacactgttc gttgttgttg taatcattcg atcactcctc
     8461 tcttttaatt aaagtataac aaacttcaag aagcactaca catattttcc caaaattaga
     8521 aatttattat atacttgaat tgttcattaa tgaaagcgaa accaatcgtt agcaactatg
     8581 taaaagaaag gaagattctt gtgaaaaaaa gtattttgct tatttctcat agtcaaaaag
     8641 taacagatgg tattaaagaa atgattgagc agatgcaaaa atccgaagag gtgcaaattt
     8701 tttctctcgg cggaataaat ggcgagatcg gatcagaccc gatgaaaatc gtagaagcag
     8761 tcaatgaatc aagcgatgca gctagttatt ttgtttttgc ggatatcggt agcgcggtat
     8821 tgaactcgga actggcaaaa gacatgttgg aagaagagca gcagcaacgt tatcatctgg
     8881 tggatgcgcc tttagtggaa ggagcttttg cagcagccat cactgccggt ataacagatg
     8941 atactcagca aatcttaaca gaagcacaaa atgccggaaa gaaaggatgg gaataaattg
     9001 aaaaaaatca tgaatgatcc aagcaatatt gtcgaagaaa tgctggaagg attagtaaaa
     9061 agctatccag aacttgttca tcgtgtagaa tcttcccgtg ttgtagccaa gaatcagaaa
     9121 gcggaacaag ttggtttggt ttctggtgga ggaagcggac atgaaccatc ccatgccggg
     9181 tttgtagggg aagggatgtt aagtgcagcg gtgcttggtg atgtgtttac ttctccaact
     9241 ccagatcaga ttcagacggc tattaaggaa gccgatagtg gaaaaggagt tttgctgatc
     9301 gttaagaatt atacagggga tgcattgaac ttcgatatgg caaaggaatt agctgctatg
     9361 gacgatattg aagtagaaag tgtgatcgta gatgatgata tcgccgttga aaatagtacg
     9421 tatactgctg gaaaacgcgg agttgcaggt actgtccttg tacacaaaat cgtgggcgat
     9481 gcagcaagaa acggtgcttc cctagctgag ttgaaagaac tgggagaaaa ggtcgtacag
     9541 gcaactaaga cgatcggtct agcgcttcga gcagcaactg taccagaagt agggaaacca
     9601 ggattcgaac taggagaaga tgagatcgag tatggtgttg gtatccacgg tgagccaggc
     9661 tatcgtcgag aaaagatgca gccttctaaa gtactggcaa aggaacttgt tactaagatt
     9721 ttagatgatt actcgaattt accaaaagaa gcaggtgtac tagtcaacgg aatgggcggg
     9781 acgcctttga tggaacagtt cgtgttcatg aatgatgttt tagctctgtt ggaagaaaga
     9841 ggcgtcaatg tagttttccg aaaagtcggt aattttatga cctcactaga tatgcaaggt
     9901 ctttctctaa cactgatcga cttgacggag acacaatgga aagattcttt agaaagcaat
     9961 gtacagacga tttcatggta aaaaatctca agataaaagg gtgataatat gaaactaacc
    10021 gttcaagaaa tccaagaatg gctcacgcaa tttgcagagg caatcaatca gaacaaacaa
    10081 tatctcagtg atctagatac cccgattggc gatggagacc atggaaataa catgggaaga
    10141 ggggtttcag cttatgaaga agcttttcaa gccgatcatc ctgaaacaat cagcgataca
    10201 ttcaaagtat tctctatggc gatgatatcg aaagtcggtg gggcttctgg tccgttatac
    10261 ggatctgcat tcatgaacat gatgaaagcg acaaaagagg tcgattctat tgattctcaa
    10321 gaaaaattag gcgaagtgat cgagcaggga acagcgggta ttcaaagccg aggaaaggca
    10381 gaagctgacg ataaaacgat gttagatgtt tggctgccag tcacagaggc attgaaggca
    10441 gggaatttaa caaaagaaat cattgaccag gcaaaagaac acaccaaagt cttggtagct
    10501 aaaaaaggac gagcttccta tctaggagag cgtgcaatcg gacatatcga cccaggagct
    10561 gcttctagtg ccattttatt ttcaacgctt ttagatgtga tcaatcaacc ttaagttcaa
    10621 taaaactgcg gtgatgctca tcgcagtttt ttgtttggct gttcttatga cttgatgcag
    10681 aacagctttt tcacaacctc tatttgtcca tatcgcaaaa ttcaggtaga atacaaaaga
    10741 taacggaaag aggcgaaaca atggaacttg gaaaatcaga ttatcagttg tttgatcgac
    10801 caatctacgc attcaaacag ctcaaagaga gccatccaac agataagatt gaacaaatca
    10861 agaaagaata taaagaacac tggcaaaaat ggaaagagat tcagctccag acagctgcac
    10921 ttttgcctga tatgtacggc atgagcaaac ctaaaatcga aagctggacg aatggctgga
    10981 atttacgcag tcatttttgg agcgcgtacc gaagcgaatc tcgtcaagac gagaatgcct
    11041 gtctagcagt tttgttgaat caaaaacaat accaaatcta tttgatgtac caacattata
    11101 aaagcgaaga acgatatggt agcattgcag cgtataacca gttgcttgcg atactgaaag
    11161 agtggagcaa aacagtcgat atcaaggatt attacatatg gccgcaacca gaacatgaac
    11221 ttgacgacca cttagctttg tctgactatt tgtccgatac aaagaaacaa gaagagttga
    11281 aaaaagcgat gagagaccga acctttcaaa tgggtaaact attcttctat ccgcaagaaa
    11341 tcgaacacgt tgagcaaata accgctgaaa ccttacaaga actagcacca ctatatcaaa
    11401 agctaggtgc ggaaaaattt caatagacaa cacgaaataa gcaatgcgaa aaacaaacct
    11461 tactctcggc ttgcgagtga aggtttgttt ttg
//
LOCUS       DACEPD010000083        11340 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.087, whole genome shotgun sequence.
ACCESSION   DACEPD010000083 DACEPD010000000
VERSION     DACEPD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.087"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..936
                     /locus_tag="IW797_002197"
     CDS             <1..936
                     /locus_tag="IW797_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1224967.1"
                     /translation="GRWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESL
                     AIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADH
                     IESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVA
                     MLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFV
                     TQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQC
                     LISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3122
                     /locus_tag="IW797_002198"
     CDS             939..3122
                     /locus_tag="IW797_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1224968.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3119..4120
                     /locus_tag="IW797_002199"
     CDS             3119..4120
                     /locus_tag="IW797_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1224969.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4135..5049
                     /locus_tag="IW797_002200"
     CDS             4135..5049
                     /locus_tag="IW797_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1224970.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5294..5410
                     /locus_tag="IW797_002201"
     CDS             5294..5410
                     /locus_tag="IW797_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1224971.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5426..7345
                     /gene="tet(M)"
                     /locus_tag="IW797_002202"
     CDS             5426..7345
                     /gene="tet(M)"
                     /locus_tag="IW797_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1224972.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7443..8816
                     /gene="tet(L)"
                     /locus_tag="IW797_002203"
     CDS             7443..8816
                     /gene="tet(L)"
                     /locus_tag="IW797_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ1224973.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9380..10642
                     /locus_tag="IW797_002204"
     CDS             9380..10642
                     /locus_tag="IW797_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ1224974.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10887..>11340
                     /locus_tag="IW797_002205"
     CDS             10887..>11340
                     /locus_tag="IW797_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ1224975.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKI"
BASE COUNT         3735 a         1897 c         2376 g         3332 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt caatctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtaa ttatgaaaaa taccaaagat
      121 tctgaatcac tggctattga tattttgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtgcgaggg
      241 ttgatgaaag tgattgaaga attacgggtt gagaatacgc cactaagtac cagcatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcccatc tgctttttag tgatggttat
      361 gtagagcagt ctatcagcct tgaaaaacaa ctgaacatta tacaggttgc cgacttggta
      421 cttcctgaca aggaaacttc ctttgaggaa tataccacaa tggagttact atcggtagca
      481 atgctgattg tcattagtac ctttgcgttg gactttatcc atacagaccg aagtattttc
      541 aagattgtag acttagacga agcatggagc ttcttacagg tggcacaagg gaaaacacta
      601 tctatgaagc tggttcgtgc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagatg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgccttt
      721 cgttccactg accttaacga gattaaaaaa acccttgcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atattaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggctacc gtggaactgg tcggacggaa
     1201 ttggaaaaca agtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtc
     1261 tttatgtttc caatgcgacg ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggcaagaata tgcagaccct tgcaggggtt acgcctagtg
     1381 gactatcaac agagggtttc tatgttggat tccttttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacagga ctgataaaga gagaaaccac aaaggcaatt catgccctca
     1501 tgaattttgt gctggttttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagcaacgc tagtttgtca ctgggtacaa
     1621 aaattgtcat gccccattca gatagtcaag gcaaggacag tgtggacttg atacgtgaca
     1681 gcctattttc catacaggtt cagcaaccgt ggctactgct tcaatataac agttcagaca
     1741 ttgaaagtat tggtattgac cgtgtggaaa gcctgctctc aaccagtcca gattccaaca
     1801 atggggaaga cagagaaaaa attgttgcgg aagaaattga agatagaagc aataccaata
     1861 tgacgattac aaagacgatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttta
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtatgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ctccgtgact gctttagggg ctgggtctgc cattgccagt gctacgggta
     2401 aaaaaggaca gtcgggttcg ggaagttctg cgagtacaca agcagatcac tctcgaccaa
     2461 acgggcagga aaaatcaaca ctaggaaagc gtatcggtca aaccatcggt acagtagctg
     2521 ataccaaaga cagaatggta gatactgctg gtaatttgaa agaacaggtt aaggatttgc
     2581 cgaccaatgc aagatatgca gtatatcaag gaaaatctaa agtgacggat aatgtccatg
     2641 atttaaccag aagtatttct caaaccaaag cggataaagc cagtggacgc aagaaacagc
     2701 aggaacaaag acggaaaacc attgcggagc gtcgctctga aatggaacag gtcaaacaga
     2761 aaaaacagcc agcttcttcc gttcatgaaa gaccagccac taagcaagaa caatctcatg
     2821 atggacagac ttcaaggcaa acaactgtac aggcttccta tagggaatct caacaagcca
     2881 aacaagagcg tccaacagtt aagtccgatt cttcaagttt aaaaacggaa cgtcaaagta
     2941 atacagttca agaaagaacg gttcaaaagc cagtaacttc aactgcacca acagatagag
     3001 cttcacaacg ttcaatcaca aaagaacgtc agtctactgt tcaaagagta ccactacaaa
     3061 atacaaaaag tagaccacca atcaaaaccg ccaccattaa gaaaggcagt aagaaaccat
     3121 gaagctgaaa actttagtga ttggtggttc gggattattc ttgatggtct tctcactgct
     3181 tctgtttgtc gccattttat tttcagatga acaagatggc ggtttttcca acattcacta
     3241 tggcggtgtg gatgtttccg cagaagtgct ggctcataag cctatggtag aaaaagttgc
     3301 caaagaatat ggaattgaag aatacgtcaa tatacttctt gcgattatac aggtggaatc
     3361 gggtggtaca gcagaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
     3421 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgagt tattagccag
     3481 tagcgaaagg cttggtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
     3541 tttcttagga tatgtggcta atcgtgggaa taaatatacc tttgaactgg ctcaaagttt
     3601 ctcaaaagag tattcgggtg acgaaaaagt gtcttacccc aatcccatag ccatacctat
     3661 caatgggggc tggcgataca attacgggaa tatgttttat gtgcaactgg taacgcagta
     3721 tcttgtcaca acagagtttg atgatgatac agtacaagcc atcatggacg aagctctgaa
     3781 atttgaaggc tggaagtatg tctatggcgg agcttccccg actacctctt ttgattgtag
     3841 cggactgaca caatggacgt atggaaaagc tggaatcaac ttaccacgaa cagcacaaca
     3901 gcaatatgat gtaacccggc atatcccatt attggaagca aaagccggcg atttggtttt
     3961 ctttcattct acctataacg ctggctctta tattactcat atcgggattt atttaggcga
     4021 taaccgtatg tttcatgcag gcgaccctat cggttatgcc gacttaacaa gctcttactg
     4081 gcaacagcat ttagtgggag caggacgaat caaacaataa gaaaggacga ttcaatgatg
     4141 aaattcggaa aaaatcagaa tacagaaaaa cagacaccaa aggaaaagaa acctcgtgtc
     4201 tataaggtca atcctcgtaa aaaggttgtg attgtcctat gggtactttt aggacttagt
     4261 ttcagctttg cgatattcaa gcactttaca gccattgata cccataccat tcatgaaacc
     4321 accatcatag aaaaggaata tgtcgatact catcatgtgg aaaattttgt agagaacttt
     4381 gcgaaagtct actattcatg ggaactaaac gacaagtcca ttgataatcg catagaaaac
     4441 ctgaaagcat atctgacaga ggaacttcaa gctctgaatg tcgatacagt tcgtaaagat
     4501 attcctgtat cgtcttctgt aaaaggattt cagatatgga cggtagaagc agatggcgac
     4561 aatcaatttg atgtaaccta cagtgtagac caactcatta cagaggggga aaatacaaag
     4621 accgtccact ctgcttatat agtgagtgtc tatgtagaca gtactggaaa tatggtcctc
     4681 attaagaatc cgaccattac tagcatacca aagaaatcag actataaacc aaaagctatt
     4741 gaaagtgagg gtacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg
     4801 ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg
     4861 atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc tatttacaat
     4921 cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa
     4981 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
     5041 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
     5101 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt
     5161 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
     5221 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgcacc cagtttaaga
     5281 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
     5341 ggttatgcat aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt
     5401 gggcttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     5461 tgatgcggga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
     5521 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
     5581 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
     5641 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
     5701 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
     5761 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
     5821 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
     5881 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
     5941 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
     6001 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgctc
     6061 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
     6121 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
     6181 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatgg
     6241 cggaatcctg catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     6301 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     6361 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     6421 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     6481 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     6541 cccgcttcta caatattatg tggattctac gacacatgaa attatacttt ctttcttagg
     6601 gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
     6661 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
     6721 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
     6781 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     6841 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     6901 gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     6961 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     7021 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     7081 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     7141 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
     7201 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     7261 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     7321 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
     7381 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa
     7441 ttatgaagtg taatgaatgt aacagggtta aatacaacca aattttaatt tggctttgcg
     7501 ttttatcttt ttttagcgta ttaaatgaaa tggttttgaa cgtctcatta cctgatattg
     7561 caaatgattt taataaacca cctgcgagta caaactgggt gaacacagcc tttatgttaa
     7621 ccttttccat tggaacagct gtatatggaa agctatctga tcaattaggc atcaaaaggt
     7681 tactcctatt tggaattata ataaattgtt tcgggtcggt aattgggttt gttggccatt
     7741 ctttcttttc cttacttatt atggctcgtt ttattcaagg ggctggtgca gctgcatttc
     7801 cagcactcgt aatggttgta gttgcgcgct atattccaaa ggaaaatagg ggtaaagcat
     7861 ttggtcttat tggatcgata gtagccatgg gagaaggagt cggtccagcg attggtggaa
     7921 tgatagccca ttatattcat tggtcctatc ttctactcat tcctatgata acaattatca
     7981 ctgttccgtt tcttatgaaa ttattaaaga aagaagtaag gataaaaggt cattttgata
     8041 tcaaaggaat tatactaatg tctgtaggca ttgtattttt tatgttgttt acaacatcat
     8101 atagcatttc ttttcttatc gttagcgtgc tgtcattcct gatatttgta aaacatatca
     8161 ggaaagtaac agatcctttt gttgatcccg gattagggaa aaatatacct tttatgattg
     8221 gagttctttg tgggggaatt atatttggaa cagtagcagg gtttgtctct atggttcctt
     8281 atatgatgaa agatgttcac cagctaagta ctgccgaaat cggaagtgta attattttcc
     8341 ctggaacaat gagtgtcatt attttcggct acattggtgg gatacttgtt gatagaagag
     8401 gtcctttata cgtgttaaac atcggagtta catttctttc tgttagcttt ttaactgctt
     8461 cctttctttt agaaacaaca tcatggttca tgacaattat aatcgtattt gttttaggtg
     8521 ggctttcgtt caccaaaaca gttatatcaa caattgtttc aagtagcttg aaacagcagg
     8581 aagctggtgc tggaatgagt ttgcttaact ttaccagctt tttatcagag ggaacaggta
     8641 ttgcaattgt aggtggttta ttatccatac ccttacttga tcaaaggttg ttacctatgg
     8701 aagttgatca gtcaacttat ctgtatagta atttgttatt acttttttca ggaatcattg
     8761 tcattagttg gctggttacc ttgaatgtat ataaacattc tcaaagggat ttctaaatcg
     8821 ttaagggatc aactttggga gagagttcaa aattgatcct ttttttataa caggaattca
     8881 aatctttttg ttccattaaa gggcgcgatt gctgaataaa agatacgaga gacctctctt
     8941 gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa gacaataaaa
     9001 attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa aataaaaatt
     9061 ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac tacctgtccc
     9121 ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg gcagagggca
     9181 ggtttttttg tttctttttt ctcgtaaaaa aaagaaaggt cttaaaggtt ttatggtttt
     9241 ggtcggcact gccgacagcc tcgcagagca cacactttat gaatataaag tatagtgtgt
     9301 tatactttac ttggaagtgg ttgccggaaa gagcgaaaat gcctcacatt tgtgccacct
     9361 aaaaaggagc gatttacata tgagttatgc agtttgtaga atgcaaaaag tgaaatcagc
     9421 tggactaaaa ggcatgcaat ttcataatca aagagagcga aaaagtagaa cgaatgatga
     9481 tattgaccat gagcgaacac gtgaaaatta tgatttgaaa aatgataaaa atattgatta
     9541 caacgaacgt gtcaaagaaa ttattgaatc acaaaaaaca ggtacaagaa aaacgaggaa
     9601 agatgctgtt cttgtaaatg agttgctagt aacatctgac cgagattttt ttgagcaact
     9661 ggatccagga gaacaaaaac gattttttga ggaaagttat aaattatttt ccgaacgata
     9721 tggcaagcaa aatattgctt atgcaacagt tcataatgat gagcaaaccc ctcacatgca
     9781 tttaggtgtt gtgcctatgc gtgatggaaa actgcaagga aaaaatgtgt ttaatcgtca
     9841 agaactgtta tggctacaag ataaattccc cgagcatatg aaaaaacagg gttttgagtt
     9901 gaagcgtggt gaacgtggct ctgaccgtaa acatattgag acagctaaat ttaaaaaaca
     9961 aactttggaa aaagagattg attttctaga aaaaaattta gcagttaaaa aagatgaatg
    10021 gactgcttat agcgataaag ttaaatcaga tttagaagta ccagcgaaac gacacatgaa
    10081 aagtgttgaa gtgccaacgg gtgaaaagtc catgtttggt ttgggaaaag aaataatgaa
    10141 aacagaaaag aaaccaacca aaaatgttgt tatatcggag cgtgattata aaaacttagt
    10201 gactgctgcg agagataacg ataggttaaa acagcatgtt agaaatctca tgagtactga
    10261 tatggcgaga gaatataaaa aattaagtaa agaacatggg caagttaaag aaaaatatag
    10321 tggtcttgta gagcgattta atgaaaatgt aaatgattat aatgagttgc ttgaagaaaa
    10381 caagtcttta aagtctaaaa taagcgattt aaagcgtgat gtgagtttaa tctatgaaag
    10441 cactaaggaa ttccttaagg aacgtacaga cggcttaaaa gcctttaaaa acgtttttaa
    10501 ggggtttgta gacaaggtaa aggataaaac agcacaattc caagaaaaac acgatttaga
    10561 acctaaaaag aacgaatttg aactaactca taaccgagag gtaaaaaaag aacgaagtcg
    10621 agatcaggga atgagtttat aaaataaaaa aagcacctga aaaggtgtct ttttttgatg
    10681 gttttgaact tgttctttct tatcttgata tatatagaaa taacgtcatt tttattttag
    10741 ttgctgaaag gtgcgttgaa gtgttggtat gtatgtgttt taaagtattg aaaaccctta
    10801 aaattggttg cacagaaaaa ccccatctgt taaagttata agtgaccaaa caaataacta
    10861 aatagatggg ggtttctttt aatattatgt gtcctaatag tagcatttat tcagatgaaa
    10921 aatcaagggt tttagtggac aagacaaaaa gtggaaaagt gagaccatgg agagaaaaga
    10981 aaatcgctaa tgttgattac tttgaacttc tgcatattct tgaatttaaa aaggctgaaa
    11041 gagtaaaaga ttgtgctgaa atattagagt ataaacaaaa tcgtgaaaca ggcgaaagaa
    11101 agttgtatcg agtgtggttt tgtaaatcca ggctttgtcc aatgtgcaac tggaggagag
    11161 caatgaaaca tggcattcag tcacaaaagg ttgttgctga agttattaaa caaaagccaa
    11221 cagttcgttg gttgtttctc acattaacag ttaaaaatgt ttatgatggc gaagaattaa
    11281 ataagagttt gtcagatatg gctcaaggat ttcgccgaat gatgcaatat aaaaaaatta
//
LOCUS       DACEPD010000084        11239 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.088, whole genome shotgun sequence.
ACCESSION   DACEPD010000084 DACEPD010000000
VERSION     DACEPD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.088"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            85..1131
                     /gene="gmd"
                     /locus_tag="IW797_002206"
     CDS             85..1131
                     /gene="gmd"
                     /locus_tag="IW797_002206"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAQ1224976.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            1211..2224
                     /locus_tag="IW797_002207"
     CDS             1211..2224
                     /locus_tag="IW797_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224977.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            2261..3205
                     /locus_tag="IW797_002208"
     CDS             2261..3205
                     /locus_tag="IW797_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAQ1224978.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWKNGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            3273..4586
                     /locus_tag="IW797_002209"
     CDS             3273..4586
                     /locus_tag="IW797_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ1224979.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVISASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            4670..5572
                     /locus_tag="IW797_002210"
     CDS             4670..5572
                     /locus_tag="IW797_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAQ1224980.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSVSFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            5577..7229
                     /locus_tag="IW797_002211"
     CDS             5577..7229
                     /locus_tag="IW797_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ1224981.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEKVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKADSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            7253..8119
                     /locus_tag="IW797_002212"
     CDS             7253..8119
                     /locus_tag="IW797_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1224982.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(8161..8352)
                     /locus_tag="IW797_002213"
     CDS             complement(8161..8352)
                     /locus_tag="IW797_002213"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224983.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCGKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            8382..8921
                     /locus_tag="IW797_002214"
     CDS             8382..8921
                     /locus_tag="IW797_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224984.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGADVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            9049..9402
                     /locus_tag="IW797_002215"
     CDS             9049..9402
                     /locus_tag="IW797_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224985.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            9869..10345
                     /locus_tag="IW797_002216"
                     /pseudo
     CDS             9869..10345
                     /locus_tag="IW797_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            10345..10539
                     /locus_tag="IW797_002217"
     CDS             10345..10539
                     /locus_tag="IW797_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224986.1"
                     /translation="MAETRPKGYPKLKEYTPNRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            10545..10830
                     /locus_tag="IW797_002218"
                     /pseudo
     CDS             10545..10830
                     /locus_tag="IW797_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            10844..11210
                     /locus_tag="IW797_002219"
                     /pseudo
     CDS             10844..11210
                     /locus_tag="IW797_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
BASE COUNT         3155 a         2303 c         2854 g         2927 t
ORIGIN      
        1 aattatcgtg tattcttgaa aagtattaga aattacgagc catttatatg gtcttatcac
       61 ttacatagga aaggataatg tatcatgaaa aaagcgctga ttactggtat tactggtcag
      121 gacggttcct atctggcaga tttcctgcta gagaagggct acgaagttca cggtatcact
      181 cgccgtgctt ctatttccaa taccgctcgc attgaccatt tgatgggcaa gatcaccctt
      241 catgacggtg acctgtccga ctcatcttct ctgattcgta tcatcagcat tgttcagcct
      301 gatgaaatct ataacctcgc tgcacagagc catgttcagg tttcttttga tgttcctgag
      361 tactccggcg atgttgatgc tttgggcgtt atgcgtattc tggaagcttg ccgtattctg
      421 ggcctgacga agaagactaa gatttatcag gcatctactt ctgagctgta tggcaaggtt
      481 gaagaagttc ctcagcgtga aaccactccg tttcatcctt acagccctta tgctgttgca
      541 aagcagtatg gcttctggat cactaaggaa tatcgtgaag cttatggcat gttcgctgtc
      601 aacggtattc tgttcaacca tgaatccgaa cgtcgcggtg agaatttcgt cacccgtaag
      661 atcaccctcg ctgccggccg cattgcagaa ggcctgcagg accatctgga actgggcaac
      721 atggactccc tgcgtgactg gggctatgca aaggactatg ttgagtgcat gtggatgatt
      781 ctccagcatg agactcccga ggacttcgtg atcgctaccg gcgagcagca cactgttcgt
      841 gacttcaccg agaaggcatt cgcagcaaat ggcatgacca ttcgttggga aggcactggc
      901 attgatgaaa agggctacga tgttgaaacc ggcaagatgc tcgtttgtgt aaatccacag
      961 tggttccgtc cgactgacgt tgacaacctg tggggcgacc cgactaaggc aaagaccgtt
     1021 ctcggttgga atccgcagaa gacctcttac gaggaactgg ttaacatcat ggcaaagcat
     1081 gaccgtgaat tggcaaagcg cgaagctgca atgaagaacg cagtcaagta agctgacatt
     1141 gaataaacat atctgtttct atctttccag tcacggcttc ggacatatcg cgcggaatct
     1201 gcccattatt atggcaacac tctgccgatg cgaagcgaag attacactgg tttgcggcgc
     1261 aaatcatctg gattttgcaa agcgcaattt ggagcaggaa ctgcccaaag agctgataga
     1321 ccgcgtgcgc tatcgtccaa taaacacaga tatcggtctg attaaccgtg agggcaccct
     1381 actggtggac acgggtaagc tgaccgagat ctgtgaatat tttttggcaa aggttccttc
     1441 tttggcaaag acagaagccg aatggctcaa gcgagaagct gtcagcgcag tgctttgcga
     1501 tatgccaatc tgggcaatgg atgcctgcga tatggcagga gtacctttcc tgtatgtagg
     1561 aaacttcact tggacagagc tgtatcgcga atttttaccc gaacatattt ggaaagaata
     1621 tgccaagcgt tacggtaaaa ttcagcacac catgctttat gcgctaaata acgaagaaat
     1681 gttggaattt ctccaaaaca gcgaaaaaac accgacttcc gttgtggcgc gtccgattca
     1741 taacgacgaa gtgcaacgca ttaaatcaca gcatgaaaaa ccgcctgtat ttgtcgcact
     1801 cggtatgtcc gcgcggttca aacagccaat tgatgtcagt cacgcgccgt atgatttcat
     1861 tacaacgcta ggtgttccgc ttgtgggaga aaacgtaacc gttatttcta acagcacccc
     1921 caatacacag gactatatag cggcggcaga ttacgttatt acaaaggctg gatggggaac
     1981 tgttgcggag tgtattctcg cacagcgtcc gatggcactg ttcgcccgcg atacagtttt
     2041 ggaagataga acaactattt gtaaattgca aaaactgggt ctggcaattt ccgtcacacc
     2101 ggaaaacctc caccgcgtag agcgggtgat tgaggacctc aaacagctag acacctcaaa
     2161 ttttgataca ttttatgatg ctgcgagcga aatcgcagac aaattgattt ctctcatgca
     2221 atgagagatg ctctaaattt agaaaggagc aagcattatt atgatggaaa aagacgcaaa
     2281 aatctacgta gccggtcatc gtggcatggc tggttccgca atcgtgcgcg agctgaaccg
     2341 tcaggactac aacaatatta tcacccgcac ccataaggag cttgacctgt gccgtcagga
     2401 cgctgttgaa gcatttttcg cacaggaaaa gccggattat gtatttctcg ctgctgcaaa
     2461 ggttggcggt attatcgcaa accagaacgc tctggcagac ttcatgtatg aaaacatgat
     2521 tctggaaatg aacgtcatca actccgcatg gaagaatggc tgtaagaagt tacagttcct
     2581 cggttcttcc tgtatctatc cgcgtatggc accgcagccg atgccggaaa gctgtctgct
     2641 gacaagtgag ctggaaaaga ccaacgaggc ttatgcactg gcaaagattt ctggcctgaa
     2701 gtactgtgag tttctaaaca agcagtacgg tactgactat atctctgtta tgcctactaa
     2761 cctgtatgga cctaacgaca actatcatcc tactcacagt catgtgctcc ccgctttgat
     2821 tcgtcgcttc catgaggcta aggaagcagg tctgcctact gttacctgct ggggtgatgg
     2881 ttctcctctg cgcgaattcc tatatgttga cgatctggca aacctgtgtg tgttcctgat
     2941 gaataactat agcggtgacg agactgttaa tgcaggtact ggcaaggaac tgtctatcaa
     3001 ggaactgacc gagatggtag caaaggttat tggttacgaa ggcgagattc tgtgggatac
     3061 cagcaagccg aacggtacac ctcgtaagct gctggatgtt tccaaggcaa ccaagctggg
     3121 atggacatat aagactgagt tggaagatgg catccgtctg tcctacgagg acttcctgaa
     3181 taacccgatg cgtgctgaac gctaatttac cgaggttgct gtgatttttg aggattgcag
     3241 cagcctcttt cttataaaaa ggagagatca ttatgaatat tgttttactg tctggcggtt
     3301 ctggtaagcg cctttggccg ctgtctaatg acatccgttc caagcagttc attaagattt
     3361 tcaaaactga agatggatat gaatccatgg ttcagcgtgt ctaccgccag attaagaagg
     3421 tagataacga cgcaactgtt acgattgcaa cctctaagac acaggtttct gctatccata
     3481 accaacttgg tgaggatgtg ggtgtatctg tagagccttg tcgccgcgat accttcccgg
     3541 caatcgcatt ggctaccgct tatctggtag atgtgatggg cattgatcca gaagagtccg
     3601 tagttgtttg cccggttgac ccttacgtag aagatgacta ttttgaggcc ctgaaatctc
     3661 tgtccgatca ggctgacaaa ggcgaagcta atctggtgct catgggtatt gaaccgactt
     3721 atccttccga gaagtacggc tacatcattc ctgagagcgc agagcagact gctttcgtaa
     3781 ataccttcaa ggaaaaacct tctgaggctg tggcaaagga atatatcgca cagggtgcac
     3841 tgtggaacgg tggtgtgttt gtctacaagc tgaagtatgt catggagcgt gcacatcagc
     3901 tgattgactt tgtagattat caggatctgt tcaacaaata cgatactctg actaagattt
     3961 ccttcgacta tgccgttgtt gagcatgagg acaagattca ggtacagcgt tttgctggta
     4021 tgtggaagga tctggggact tggaatactc tgaccgaagg tatggaggaa tccattatcg
     4081 gtaaaggtgt tatgaatgag aattgcgatg gcgtacacat cctgaacgaa ctggatgttc
     4141 ctgtgttggc tatgggtctg catgatgtgg tcatctctgc atctcgggaa ggtattctgg
     4201 tttccgataa ggaacagtcc agctatatta agcctttcgt tgacaagttc gagcagcaga
     4261 tcatgttcgc agagaagtct tggggtagtt ataaggttat ggatgtagaa gcagagagca
     4321 tgaccattaa ggttactctg aatgctagtc actccatgaa ttatcacagc cacaagaacc
     4381 gtgacgaggt gtgggtggtt ctggccggta ctggtaagac catcgtggat ggatatgagc
     4441 aggttgttaa ggctggcgat gtgatcacca tgagtgctgg ctgtcggcat accattatgg
     4501 cagatactga gctgaagctg attgaagtac agcttggcaa ggatatcaat gttaaagata
     4561 aacagaaatt tgagttagag tattaaaaaa cggaggaggg ctcgttatac agaaaccgtt
     4621 tttattgaag ccttccgcaa aggattatct gtggggggga accgcctgaa tgatgaattt
     4681 gcaaaaggaa ttgagatgtc tcctcttgcg gaaacttgga atgctctacg cacccggatg
     4741 gaccgagctt tgttgtgtct ggtcagagta tcaggggatg accttgacgg aggtgttgca
     4801 agaacaccca gagtttctgg gaacgcatcc gcagaccaaa ggagaattgc cgatcctgat
     4861 caaattcatt gatgcgaaaa aggatttgtc tgttcaggta catcccacag atgaatatgc
     4921 aattgaaaaa gaaaacgggc agcttggaaa gacagagatg tggtatgtac tggatgccag
     4981 cttaatctat gggctgcaga aaataacaga agaagctgtg atccgtgaag cgattgctca
     5041 gggaactgtt gagcgttatc ttcagaaggt tccggtcaaa aagaatgatg tgttctttat
     5101 tgaggcggga acgattcatg cgattggtgc gggtgcacta attgcagaaa ttcaggaaaa
     5161 ttccaatctg acttatcgtc tctacgatta tgaccgtgtt gacaaaaatg gtaaaaagag
     5221 agaacttcat gtagacaaag cgctagaagt agcgaacttg aaagggttgt cagaaccgaa
     5281 acagccgctt cgtgttctaa aatatcgtgc tggtgtagct gcggaattgc tttgcagatg
     5341 caagcatttc gaggtctctc gtatgattgt caacactgag agaagacaga cggtacacta
     5401 tcgtgcggat tcagtcagct tccgtgtgtt gctgtgtgtc aatggctgtg gtaccgttcg
     5461 atttgataat gaatcgttgg acatctataa gggggactgc ctttttgtgc cggccgattc
     5521 tactgaaatc agcattcacg ggtcgattca gtttctggat gtgagaggat aataatatga
     5581 attataaagt agaatatgag cgttggctga atcatgccaa tgcagatatt atagaagaac
     5641 tgcgttccta ttctgagaag gacattgagg actctttcta taaaaatttg tctttcggta
     5701 ctggtggtct gcgtggtacc atcggtgcag gcactaatcg catgaacgta cacaccgtag
     5761 gtaaggcaag ccaaggtctg gcagactatc tgaataagaa ctatgaagcc gcttccgtgg
     5821 tggttggcta tgattcccgt atcaaatctg atgtgtttgc aaagactgcc gcagacatct
     5881 ttgctgcaaa tggtattcag gtgcacttgt ggccagaact gcttcctgtt cctacagtat
     5941 cttttgcgac tcgttatctg aaggcagatg ccggtgttat gattacggca tctcataacc
     6001 cttctaaata taacggctac aaggtatacg gtgcagacgg ctgtcagatt actaccgagg
     6061 ctgctactgc aattctggca gaaatcgaaa agctggacat ctttgctgat gtaaagatgg
     6121 atggcgatgc ttctaagatt gcatccattc agtcggaagt tctgactgct ttcatcgaag
     6181 aagtgaaggg gcagtccgtc ctgtttggcg aaaaagtgga caagaatgtt gctatcgtgt
     6241 attctcctct gaatggtacc ggtctggtgc ctgttacccg caccctggca gagatgggat
     6301 acagcaatgt gaccgttgtg gaagaacagc gtctgccgga cggtaatttc ccgacttgtc
     6361 cttatccgaa ccctgaaatt aaggaagcta tgtctctggg tatggagtat gccaagaagt
     6421 acaatgcgga tctgctgttg gccactgacc ctgactgcga ccgtgttggt atcgcagtta
     6481 agagcgcaca tggtgactat gttctgttgt ctggtaatga gactggtctg ctacttctgg
     6541 actacatctg ttctcagaga attaagcatg gaaaaatgcc tgctgaccct gtgatggtca
     6601 agaccatcgt caccatggat ttgggtgaac gtatcgcatc caactatggt gttcgtacca
     6661 tcgatgtgct gaccggcttt aagtttatcg gtgagcagat tggtatgttg gaaaaggctg
     6721 gtaaggctga cagctatatc ttcggctttg aggaatctta cggctatctg tccggtagct
     6781 atgtccgtga caaggacggt gttaacgctg cttatctaat ctgtgaaatg ttctcttact
     6841 acaagactca gggtatcagc ctgctggata agctgaatga gatttataca aagtatggct
     6901 attgtttgaa caccctgcat tccttcgagt ttgacggtgc ggcaggtttt accaagatgc
     6961 aggaaattat ggttgaattc cacaagggtc tcgatataat tggtggtaag aaggttattg
     7021 aaactttaga ctacagtaag ggcctgaatg gtctgccgaa gtctgatgtt ttgaagttta
     7081 tgctggaaga taactgctct gttgtggttc gtccttctgg tactgagccg aagctgaaga
     7141 cctatatcag cgtcagcgca gagaacaagg aagccgccga agctatggaa gctattatgg
     7201 ctgctgaact ggcggaaatg ttcgagtaag aaaagaaagg agaaatcttg taatggagga
     7261 actgttttct aacgctttga aatcggtgaa gtcaaatcgt gtgctgtaca cggcatcgtt
     7321 ctttgcaaga tcctctcttt tgtatttgca ggaaattgga cagctgactg ctctgaaggc
     7381 acatacatct aggcgtacag gattgtcatc ttacctttgt ttcctggtag aagttggttc
     7441 aggcacattg gagtataagg ggcagaaata tacgcttcaa gtaggagata tggtgtttat
     7501 tgactgtttg aagccgtatg tccatgagtc atcagaagat gatttgtgga ctttgcggtg
     7561 gtgccatttt aatggcattg gtatgacgga agtctacaaa aaataccttg aacgaggtgg
     7621 tcaaccggtc tttaaaacat caaaaacaga taagtatgct gacttgctga ggagccttca
     7681 tatggtggca gagggacagg agtatgtccg ggatatgaaa atcaatgttc tactcagtga
     7741 actgctgatg catttgatgg aagattcttg gaatcctgac aaagtgccga atttctctac
     7801 aaaaaaaatg gaattgcaga acataaaaca atatctggat gaccattaca cggagaagat
     7861 tacgctggat cagttgagtg cagagttttt tatcaataaa cagtacttag ctaagatttt
     7921 tcgtgaaact tacggtacaa ctgttgtcgg ttatctgaat cagaaacgaa tcacacaggc
     7981 caagcacttg ctacgcttta caaatctaag tatggaagag attgcaacaa aaattggcat
     8041 aacagatgca aactattttt ccagaacttt tttcaaggtg gaaggtgttc gtcccagcga
     8101 attccgcaag atgtggtaat aaaaagccgg cggtgttgag ccgccggtca gtacctgtcg
     8161 ttaacccgca ttcaaagctt taatctcagc tttgatttca gcacgaacac gatagtaatc
     8221 ttcagtttcg atttcggtat ccagccgagt tgctgtcttt ccgcagtcag gacaacgctc
     8281 tggctgttcg tcggcaggga agctgtagtg gcaggaatca caggtgtaaa tgtgatcgtc
     8341 acgtataatc atcacctacc aatccagtgc ttagattttt catgcaatta ctattatatc
     8401 gaattggtga ttccacaaaa atccgcttct tgataaatgt acgattcaga gtgatatata
     8461 gtactgtact gccaaaggga ataccacaac ggcagaaatg gatgattcac atggaagaaa
     8521 ttatgcagat gattttaggg aacgcaaaat tccagctcgg acaggtggtt tcgacccctg
     8581 ctgtattgga aactttcaac cccctgcaga ccgcaaacgg cattcccgaa gccttgagca
     8641 acctttggtt tagacaggaa attagtattg cggagatcga ggacgcagtt ctggatgcca
     8701 agaacggcaa ggagctagca aacagcctca accgcatcaa gcttttccgc aagttcaccc
     8761 tcgaccgaga gctggacggc atggtcagac tgaagggcgc ggacgtctgg aagaacatca
     8821 gctacctcaa ggtctacacc gccacgccgc ctaagaaacc gaccccccca aggttccagt
     8881 cgaacggctg gagccttttc ttctctcata aatcggcata atgtatacaa atacagctcc
     8941 aaatgattgt gtagtatatg cctgtggatt gacttgatat agtcgagaga ctatggcaat
     9001 atacacacaa ccaaagggga aaatcccaaa aacaaggagg aaacgctaat ggcaatgagc
     9061 gaaaaggcaa agaaatattt gaaggagatc aagggtgcaa agaccatccc ggagctgaag
     9121 gatgtggaga tcgcaatcaa gcgagacggt atccttgctt gggcagagtt caccaagttg
     9181 aacgaggcgg tggaagagaa gaaggtcgcc cttcgcaaga aaaagcagga gaccagcctt
     9241 caggagatcc ttttctgggc ctacaaaaag gaatcagaca agctgctgaa gatgatggac
     9301 gagggagcag acaaggatgc catccagatg caggtccagc gttacgacag catcggccag
     9361 atcattgcag aggcagacct cgaagatgag tatgaggttt gagaatacag cctgaaccac
     9421 taagaaccaa ataccgaaag ggacttctga aacagacaga ggtccctttc ttttgctcac
     9481 tttggcggtc aattatgaac caaaaaccag tcgaatgatt gtgtacatta tggctttcaa
     9541 acgacttgat aataatcact tttagagtga tatatgtact acaaaaaaca aaagactgga
     9601 gaaaacaaca gatgagcgaa tacagaatca tttatatcag cgacgaggac ggcggcgagg
     9661 tggaaggatg tggctacacg gctacgatgg ttatgatgcg gtcagaaact tcttggaatt
     9721 tgcctgcggc atcttgaaaa tcctgcgggt gcgattcatc agaagagtgg actgaaatac
     9781 aagttagcag atagagaaga gagtcaggtt tcgacttggc tctgttctct gtctactggc
     9841 agaaatacat tgaagggagg tgacgactgt ggcaaccaga ggaagaaaat caaccccgac
     9901 agccatgaag aagctggagg gaaatccggg caatcgccca ctcaatgcaa aagagtcaac
     9961 gccggaaaag aaggcaccga cctgtccgaa gtggctggaa ccagaggcaa agaaggaatg
    10021 gaagcgtctg gcaaagcaga tggaggccat cggaatcttg acagatgtgg atatggctgc
    10081 ctttgccggt tagtgccaag cctacgcccg ctggaaggaa gccgaggaat tcattaccca
    10141 gcacggtatc atcgtgaaaa ccctatccgg ctattggcag caggtaccac aggtgtttat
    10201 tgcccagacc tatctgaaaa ttatgaaccg attttccgag cagttcggtc tgacgccttc
    10261 gtccagaagc cgtatcattg ccggtgatgg cggtggtgtc ggagcagaag atgcgatgga
    10321 ggctctgttg ggaggtggta gctgatggca gaaaccagac caaagggcta tcccaaatta
    10381 aaggaataca caccaaaccg attcatgctt tcggaatgtc actacgacaa agccagagcg
    10441 gatcgggcgg tgaactttat cggacagctc cgacacacca aaggcaaatg ggcaggcaac
    10501 cgcttctggc tcttgccttg gcaggaacag atcatctagt gtaatctcgc ttaactgtat
    10561 taacggacgt agcgacttta ttaagagtgt tcatggtagg agcattagtt gcaacaatga
    10621 ttgatattaa atttttatgg taccaacgat tggcgatgtg acattgaata tgcaaaacta
    10681 tatcgatatg atattatcta aaatcttact cgctgggatc gtcgaggtgt ttattggttg
    10741 ttaggaaaga aaaatatgac ccaactcgag cgatttttat cgtattactt gtgtgtggct
    10801 ttatctgcat tgtgtgtgat ttcgaaataa gggagagcca aaaatgagta gaaaattgat
    10861 attgattagt cacggaaaat tttgtgagga attgaaaaaa agtacaaaaa tgattatggg
    10921 acctcaagag aatatctaca cgttaccttt attaccagaa gatggcactg aaacatacag
    10981 acaaaagctc ttactgtcat agattcgtta aatgattttg tggtactatg cgatctttta
    11041 ggcggtacac ctactaatat catttctaaa ctgataatgg aaggacaaca gattaaactt
    11101 tatgtcggat taatcttcct atggtcattg aatttatcaa ctcgcaagtg attggttcag
    11161 agcccgagtt tgttcgtgca ggatgtgaaa gcattgtatc tgtcaactaa ttaattagtg
    11221 gaatagtaga agatgaaga
//
LOCUS       DACEPD010000085        10831 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.089, whole genome shotgun sequence.
ACCESSION   DACEPD010000085 DACEPD010000000
VERSION     DACEPD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.089"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            91..309
                     /locus_tag="IW797_002220"
     CDS             91..309
                     /locus_tag="IW797_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1224987.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(598..1110)
                     /locus_tag="IW797_002221"
     CDS             complement(598..1110)
                     /locus_tag="IW797_002221"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ1224988.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1124..3421)
                     /gene="recJ"
                     /locus_tag="IW797_002222"
     CDS             complement(1124..3421)
                     /gene="recJ"
                     /locus_tag="IW797_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ1224989.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3563..3997)
                     /locus_tag="IW797_002223"
     CDS             complement(3563..3997)
                     /locus_tag="IW797_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ1224990.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4007..4795)
                     /locus_tag="IW797_002224"
     CDS             complement(4007..4795)
                     /locus_tag="IW797_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1224991.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4792..5733)
                     /locus_tag="IW797_002225"
     CDS             complement(4792..5733)
                     /locus_tag="IW797_002225"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ1224992.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5910..6497)
                     /locus_tag="IW797_002226"
     CDS             complement(5910..6497)
                     /locus_tag="IW797_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ1224993.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6718..8031)
                     /gene="obgE"
                     /locus_tag="IW797_002227"
     CDS             complement(6718..8031)
                     /gene="obgE"
                     /locus_tag="IW797_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ1224994.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8319..9329
                     /gene="gap"
                     /locus_tag="IW797_002228"
     CDS             8319..9329
                     /gene="gap"
                     /locus_tag="IW797_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ1224995.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9414..10349)
                     /locus_tag="IW797_002229"
     CDS             complement(9414..10349)
                     /locus_tag="IW797_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ1224996.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            10535..>10831
                     /locus_tag="IW797_002230"
     CDS             10535..>10831
                     /locus_tag="IW797_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1224997.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER"
BASE COUNT         3046 a         2420 c         1883 g         3482 t
ORIGIN      
        1 ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata tacgaacaat
       61 tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct tggcttagta
      121 ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc agctttactt
      181 gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa gtaccgtcaa
      241 tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta cagatccagg
      301 caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa cttttacggg
      361 ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta aagccagttt
      421 ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg gtctttgctt
      481 gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct gacttttgag
      541 ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt tttctgctta
      601 atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat ggagatccat
      661 caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga tcagttcgat
      721 cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca cacgttgacc
      781 tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta atccataagt
      841 cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa aaccgactcc
      901 taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc caactaccat
      961 atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc gataagcgtc
     1021 accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg gatagtcggg
     1081 aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt gttaaccatt
     1141 tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt ttattgagcc
     1201 gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg actttttgta
     1261 aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc ataaaaatta
     1321 ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga cgtatatcaa
     1381 ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt gtgcctaccc
     1441 cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg aatgaataag
     1501 accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca actagagagt
     1561 ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa acaatcgcag
     1621 cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct aaataaaggc
     1681 taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca aatacttgca
     1741 gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg ttccattcat
     1801 tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct tcttgagtac
     1861 caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca aatttcagat
     1921 gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga aataaaaact
     1981 gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca attcgctcta
     2041 ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct ctcatcaagt
     2101 caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg acattttcac
     2161 ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat aaatctcgca
     2221 tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat cctttagctg
     2281 atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc attattttcc
     2341 cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga tggatatgat
     2401 tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt gcttttcttt
     2461 cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca tcaaacgtcg
     2521 caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt agtctgggag
     2581 caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg ctttcttcaa
     2641 atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct aatgaaacaa
     2701 ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc gctactaaat
     2761 ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat gctactccga
     2821 ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga acaatcgcat
     2881 aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg tctacaccag
     2941 cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca gtgatgatca
     3001 actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca tacccatgct
     3061 caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt gtttctttca
     3121 ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg attttttctc
     3181 cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt atcagatacg
     3241 gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt tcctcatggt
     3301 aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca cggatcattt
     3361 ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt gcttgtctca
     3421 ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa agcagaaaga
     3481 aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga tcttttgtac
     3541 gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg ggcgattgag
     3601 ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct tgtaactccg
     3661 cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc tgttcttctt
     3721 ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc tgagtcagct
     3781 gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat acaatcagtg
     3841 caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag gaagtgaagc
     3901 caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata atcaaaacta
     3961 aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac tttttgttaa
     4021 aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat tttgatgcaa
     4081 cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg cggacgatca
     4141 tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa tagcttccgc
     4201 ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact gtcgtaacag
     4261 ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg actgcaaatt
     4321 tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata gaggcgatat
     4381 taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt gtcaaaacca
     4441 tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg aatgtaagga
     4501 agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc ccgacttctt
     4561 ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct accttgaaag
     4621 cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct acaaatgcca
     4681 ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa cagatttgtg
     4741 cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa ttcatgcctc
     4801 ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg tcgtgttttc
     4861 aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat atctcgcact
     4921 gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag cagcttgatg
     4981 agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa atgtgctttc
     5041 atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt ttctcgtatc
     5101 gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt ttccattgat
     5161 cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt acagtggtcc
     5221 agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat gatcatgttc
     5281 aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat aaacagaaaa
     5341 ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca gcggataact
     5401 taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc cttttggccc
     5461 gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga gcccgggaag
     5521 tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt ttctcggacg
     5581 aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca accaaacttc
     5641 attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt ttgcaggaac
     5701 acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct ttaagttcct
     5761 ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg ggttcaaatt
     5821 aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa acagcagcga
     5881 acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact ttcattgtcg
     5941 ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt cgcatgattt
     6001 tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa gcgagtaatt
     6061 tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc ttcatcagca
     6121 atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc gaacacggtc
     6181 tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc ggcaaaccat
     6241 cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat taatccaccc
     6301 gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa ttgatgtgtc
     6361 ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg aacagcatgt
     6421 aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa atttaaaatg
     6481 attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt attttacctt
     6541 taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg gctgtgacaa
     6601 aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga tcacttctgt
     6661 cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt tgcttgccta
     6721 ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt tcgcaccgcg
     6781 tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac gcatcactgt
     6841 ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac cagataagat
     6901 ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat tgaatccata
     6961 gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg ttacttctaa
     7021 caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc cagaaattgg
     7081 aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct gttctttgaa
     7141 tttcactaga ttttccggtg catccggcat atccattttg tttgccacga tgatttgcgg
     7201 acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa gatagtcttc
     7261 ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta ctcgtgttct
     7321 ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc cttcaatcaa
     7381 gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca ttcctagatt
     7441 aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg aaataacgga
     7501 tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg ccaatacttt
     7561 caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa tttccggtgc
     7621 aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac cttttgccac
     7681 aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct ccgcatctcg
     7741 gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac cgtgcatccc
     7801 tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc gaaaatccat
     7861 caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac caccgtctcc
     7921 gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca ttccgtctcc
     7981 gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca taattaagtc
     8041 ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca aaagtcttac
     8101 ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt gtttaccttc
     8161 ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag tataattttt
     8221 tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa ttgaggatgc
     8281 cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa gtaggtatta
     8341 acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc tcagatgata
     8401 ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa ttattgcaat
     8461 ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac tcaatcgtag
     8521 tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc ccttgggcaa
     8581 aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct gaagaaaaag
     8641 caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca gctggtgcta
     8701 tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac aaaatcatct
     8761 cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta aataatgaat
     8821 ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca caaatgctct
     8881 tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct gacaatacta
     8941 tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag ctacaaggaa
     9001 aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact gaattggttt
     9061 cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa aaacacacag
     9121 ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat gtcattggaa
     9181 caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct ggtgatttcc
     9241 aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc caaatgattc
     9301 gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa agaagccttt
     9361 gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact cgttcaatga
     9421 acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc tgtcttgtat
     9481 gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt cgttgcacga
     9541 tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg tataaagagc
     9601 attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa aaagtagcca
     9661 gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg aagactaaac
     9721 atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca atcttcaatg
     9781 attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt tgctgctccc
     9841 atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag cactttgttt
     9901 atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc cccttcgctt
     9961 tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg aaacatcttt
    10021 gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag agctgttttt
    10081 tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc ttttgaagtg
    10141 atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc cgtttctttg
    10201 gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga aggcacaaca
    10261 gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc taaagcgacg
    10321 cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc ttttttcata
    10381 taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt ttataacccg
    10441 cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc aaattgtatt
    10501 tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc ttaccataga
    10561 cgaactgaca atgatagaat catattatct tcaacataat aagccggttg aaatcgctaa
    10621 ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca agcaaggcaa
    10681 gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg gtagaaaagt
    10741 cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc ttggttggac
    10801 gcctgacgtc attatcgggc gaaaagaaag g
//
LOCUS       DACEPD010000086        10777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.090, whole genome shotgun sequence.
ACCESSION   DACEPD010000086 DACEPD010000000
VERSION     DACEPD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.090"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            89..1135
                     /locus_tag="IW797_002231"
     CDS             89..1135
                     /locus_tag="IW797_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ1224998.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1484..2116
                     /locus_tag="IW797_002232"
     CDS             1484..2116
                     /locus_tag="IW797_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ1224999.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2704..4143
                     /locus_tag="IW797_002233"
     CDS             2704..4143
                     /locus_tag="IW797_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1225000.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4438..4656
                     /locus_tag="IW797_002234"
                     /pseudo
     CDS             4438..4656
                     /locus_tag="IW797_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4905..5120
                     /locus_tag="IW797_002235"
     CDS             4905..5120
                     /locus_tag="IW797_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225001.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5337..5593)
                     /locus_tag="IW797_002236"
                     /pseudo
     CDS             complement(5337..5593)
                     /locus_tag="IW797_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5780..6691)
                     /locus_tag="IW797_002237"
     CDS             complement(5780..6691)
                     /locus_tag="IW797_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1225002.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6710..7714)
                     /locus_tag="IW797_002238"
     CDS             complement(6710..7714)
                     /locus_tag="IW797_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1225003.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7711..9834)
                     /locus_tag="IW797_002239"
     CDS             complement(7711..9834)
                     /locus_tag="IW797_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1225004.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9839..10759)
                     /locus_tag="IW797_002240"
     CDS             complement(9839..10759)
                     /locus_tag="IW797_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1225005.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         3297 a         2035 c         2019 g         3426 t
ORIGIN      
        1 aacaacttcg tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag
       61 tagtacaagt gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa
      121 aaaggaagcg ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga
      181 atcaacagaa tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga
      241 caaaaatgaa tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg
      301 tcagaaatat gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag
      361 tggtcaaagc cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta
      421 taatggaaac tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac
      481 gacccaatca aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa
      541 attattaact aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa
      601 tagtaaaaca tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca
      661 atactatttg aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga
      721 agttaaaggt ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat
      781 aaccagtttg tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac
      841 acagggtgag ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta
      901 ctcttgggga aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca
      961 ccagcaacaa tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta
     1021 tgtaggaagt actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag
     1081 tagtctttct cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag
     1141 gtaatcaatc tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata
     1201 ttgagactgg aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat
     1261 cgaatatcga gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat
     1321 gaaaacaatt tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa
     1381 ttagtcataa aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt
     1441 aattgaaatt atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg
     1501 tcaccttact gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg
     1561 tgactgtggg aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag
     1621 ttgtggatgt ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt
     1681 tggtaagtta gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga
     1741 atgtcaatgt ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga
     1801 tgtaaaaagc tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg
     1861 attacttact gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg
     1921 tcattgtgag tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac
     1981 tcaaagttgt ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat
     2041 agagtgtcta acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg
     2101 ttctaaagag aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc
     2161 gtatgattct attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca
     2221 acctttgatt gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga
     2281 tatataaaca tctataatat attattcata ctattgatat ttagtataat cctatggaat
     2341 tgaatatcat agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata
     2401 aatatgaaaa aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc
     2461 acactattaa tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag
     2521 cgaattatca ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt
     2581 acatgaggat atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg
     2641 ttgggcagta gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct
     2701 attatgtata gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt
     2761 gctatatcta tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta
     2821 cctaccatct cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt
     2881 tatctgatga ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg
     2941 aaaatcaaag tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg
     3001 ttcaatcatt cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt
     3061 atgactatgg ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt
     3121 cgggcattgg gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt
     3181 ggcggcttga ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg
     3241 ggaatcgtaa cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc
     3301 catacaatcg atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga
     3361 gcaatctttt tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta
     3421 atttcagtga ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg
     3481 atttctttcg gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg
     3541 attttttctt ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc
     3601 gggttgagtc ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc
     3661 ggggcgcctt taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta
     3721 ggcttgggct tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct
     3781 ttgtggctat acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc
     3841 cccaataata cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg
     3901 atgaattcct ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc
     3961 ttatatcaag cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg
     4021 ccggatgtct ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt
     4081 gttgcctttt tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc
     4141 tgaaaaaacc actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat
     4201 ggatgatgga agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa
     4261 tactagtgct caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa
     4321 ccgcaagcta ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg
     4381 agccaacgct ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac
     4441 ttctgtcaaa aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa
     4501 tgtagacaca cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc
     4561 cgtatacatc ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa
     4621 tacaagcatt gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa
     4681 taagagatac cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt
     4741 agtcatgaga ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt
     4801 tctaattata ataagatgac cactacagta acctactgaa atatataaac agccactgta
     4861 gtaccatttt caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg
     4921 cggatatgaa tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag
     4981 gtactggtat caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa
     5041 agctgaatct tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg
     5101 agattgaaat atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa
     5161 cgatccttgg tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt
     5221 ctttactaac aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac
     5281 ttttatctaa gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt
     5341 atcagacgtg agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga
     5401 gcataatatg aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt
     5461 ctgaaattct ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt
     5521 tacaatgttt cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg
     5581 tatctaattc catggtatat caaccatttc ctatatagac tgattatgac atggattaaa
     5641 ataaaaaggc actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc
     5701 acctatagag gatgggcgac ttctgcctga aataagttac actacttata acttctaata
     5761 aaattagatt gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta
     5821 atactaattt gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct
     5881 ttgtactcac tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa
     5941 actttaagtc aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt
     6001 tttcagaagc tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg
     6061 ttgttttggc atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt
     6121 ctggctttcc aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa
     6181 agattgtcac tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt
     6241 gctccactaa aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta
     6301 cagattgaac ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga
     6361 gaccgttttc tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt
     6421 tatttttcca tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag
     6481 tattcactac cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg
     6541 tgaaattttt ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca
     6601 ccatttttcg atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt
     6661 ccttttgatt ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc
     6721 ggccagctcc gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg
     6781 gatctcctgc atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag
     6841 taccggcatc ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat
     6901 caatatgagt catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt
     6961 tggcataaca ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac
     7021 caaacacata ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt
     7081 tgtcatcaaa ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc
     7141 cataattata ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt
     7201 tttgaccacc tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc
     7261 cgtttttccc gacatgatct agatacccgc caccatagtt ataggcctga ataatactat
     7321 cttgatcaca ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt
     7381 gtttgatgga tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt
     7441 gcataacgtc ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa
     7501 tatactctgt tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata
     7561 cttcttctga gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt
     7621 cttcaccaaa caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag
     7681 ttaatgaaat aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg
     7741 acgatggttc attctttttt gtactttttg attggtctgc tttggcagag tggacctctg
     7801 tttcacttgt tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat
     7861 ttcttgacta gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt
     7921 tggtgcttct tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat
     7981 attcgccctt ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc
     8041 cattttcgca tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc
     8101 ttggaacagt ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg
     8161 gagattgtat ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt
     8221 atctttcacc aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat
     8281 tcgggattgt ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc
     8341 gtgatcgttc ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga
     8401 ttttgattgt tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc
     8461 tcgtcccaca tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg
     8521 catgacgcgt cttcccattg actggctatc attactctgg aggttgaaca tactcataat
     8581 gtcgcccaat ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa
     8641 aaatggatag gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat
     8701 caccaaagta acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt
     8761 tcctaaactt tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc
     8821 aaagataatg aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa
     8881 ggaaatgcca atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt
     8941 ggtaatactg agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg
     9001 atctttgcct ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc
     9061 aatttcttct atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat
     9121 cgaaaacaaa ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg
     9181 tacaacgatc tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt
     9241 gatttttgtg atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat
     9301 cacaaaatta atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata
     9361 cgccacatac acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga
     9421 ggtctggagt ccattttcag taataccagc caatgtttgg atattctttc caatactttc
     9481 agccgtatcc gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt
     9541 cgacaaatac aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac
     9601 gcttttccct aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa
     9661 gaaatctagt tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt
     9721 atcatcaact agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag
     9781 agtgagtatg ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc
     9841 actccattct ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga
     9901 ctggatgtac ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat
     9961 tttccaaatc tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt
    10021 ccaaggtttt cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat
    10081 tattcttcaa gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg
    10141 cattcatagc ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt
    10201 gaagaaaact ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga
    10261 taaagtccaa ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg
    10321 tggtatattc ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga
    10381 tatttaactg cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat
    10441 gggcaaaatc ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg
    10501 agccttcttt tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa
    10561 ccgtccgaat ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg
    10621 ttaaaaaggt cagaatatca attgctagac tttcagaatc cttctttttc tccataatca
    10681 cataaggatc aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt
    10741 catccgcaat ttctggcaag gtttctttcc agccacc
//
LOCUS       DACEPD010000087        10033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.091, whole genome shotgun sequence.
ACCESSION   DACEPD010000087 DACEPD010000000
VERSION     DACEPD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.091"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..524)
                     /locus_tag="IW797_002241"
     CDS             complement(<1..524)
                     /locus_tag="IW797_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1225006.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVK"
     gene            complement(691..2811)
                     /locus_tag="IW797_002242"
     CDS             complement(691..2811)
                     /locus_tag="IW797_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1225007.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            3041..4219
                     /locus_tag="IW797_002243"
     CDS             3041..4219
                     /locus_tag="IW797_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ1225008.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            4235..4993
                     /gene="aroD"
                     /locus_tag="IW797_002244"
     CDS             4235..4993
                     /gene="aroD"
                     /locus_tag="IW797_002244"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ1225009.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            5016..5501
                     /gene="ybaK"
                     /locus_tag="IW797_002245"
     CDS             5016..5501
                     /gene="ybaK"
                     /locus_tag="IW797_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ1225010.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(5572..6825)
                     /locus_tag="IW797_002246"
     CDS             complement(5572..6825)
                     /locus_tag="IW797_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1225011.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6942..8291
                     /gene="gdhA"
                     /locus_tag="IW797_002247"
     CDS             6942..8291
                     /gene="gdhA"
                     /locus_tag="IW797_002247"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ1225012.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            8403..9749
                     /locus_tag="IW797_002248"
     CDS             8403..9749
                     /locus_tag="IW797_002248"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ1225013.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
BASE COUNT         3207 a         1809 c         2083 g         2934 t
ORIGIN      
        1 tttttcaccg atttgaattc atcgaaacac accacttctg gtaacggctg gttcaaagaa
       61 acggttttag gccgaaggct gcgaagtatt cgaatgacag tggtcggaga aatgtgcttc
      121 atacgtgcaa tcaaagacat agaaataggc tcacttaata gctcaagaat ggcttgtttg
      181 actctgcggg caatggagca gcgacgatcg ctaactgaat cttctgctaa aaaagtcttt
      241 ccacagtgtt tacatttgaa gcgctgtttg ttaatccgca caatggtctt gtagttactg
      301 acatcattca ataagatgga tacttttttc cagccccatt taatgatcgt ttgcttattt
      361 tgatagtggc aatggaagca ttcctctggt tgataggtca atgtgccatc aaaaacaaaa
      421 catgtttcgt cattaatgcg ctctttgcga aaggcatctt catgaaaggt aagatttagg
      481 tctagaatat ctagtaactc tttggtaaac tctgaatagg acatcttgaa acactccttt
      541 gaatggtttt cgtcgacttt attctaaagg atttcaaggt gtctgtttgc gtttattaga
      601 aaaaagaaaa agtattggca acagaaggcg tcaccaacac tagatattat agatcctaat
      661 tttgtcgctg ctccttttaa atttatttta tcattcggaa gaacttgtgg tcgttccgtg
      721 aagttcttgg tacgtttgcg ttttataaag atcaacagtt gattgtccgt tgtgttcact
      781 aaagacacta gttgacttgt caccaagaga tttttcagct tcttttaatt tctctaagcc
      841 attcttgtag tcataatcac tggtatcaat tgggtctaat ccactattcg tatagaaccg
      901 aagaagatca ccattattga tttggtcaga aacagctaac tgtttatcaa ctttttcttt
      961 cagtgaatca atttcattct gtacttcctc agtaggttca gtaatagctt caccagtttc
     1021 gttactatag atcgtaccac cgtagtaagt atattttggt gtaacaaaat caccatcacg
     1081 gaaagaaaca agctgttcat gatctttcga gaatagatct tgtcctaact gaatataatt
     1141 ttttgtatct acacctagta agtgaagtaa tgtaggcaag gcatcaactt gtccgccgta
     1201 agtgtgattg attccgccct gtgtttgtcc agggatatgg atcatataag ggacacgctg
     1261 catttgtgca ttgtcgtagt ttgtccaagt agaacttgtt tttccaacta attcagctag
     1321 atttttattt cttgaattcg agacaccgta atggtcaccg taaaggacga tgactgaatt
     1381 atcatacaag ccgctttctt tcaaataatt gaagaattct tgtacggcgg tatctaaata
     1441 attggctgtc gcaaagtaac cgttgatcgt ttcatcattt gtattggcta acgggaatcc
     1501 tgcttcgtca ttggtgaatt ttgaataagg ataatggtta gatacagcaa tgaattttga
     1561 ataaaatggt tgttgcagac gttccaagta ttgtacggat tgttggaaaa atggtttatc
     1621 atgcaacccg tattggaacg aattgtcatc attaacatca taataagaag aatcaaagaa
     1681 ataatcatat cctaaattct tgtatgtctc gtttcgattc cagaatgtac cagcgttccc
     1741 atggaatacc gcactaatgt aaccttgtgt ttgacttaaa atatcaggtg cagcttggaa
     1801 agtatttttt ccgcctaatt gtgtgaacaa ggagccttga ttcaagccaa acaatgaatt
     1861 ttccatcagc gtttccgcat cgcttgtttt tcctgctttt acttgatgga aaaagttgtc
     1921 aaaactgaac gtgctgttgc tgtggtaaag actattgatg aaaggtgtaa cttcatgctg
     1981 gagaccattc tcatctgtca atttataatc gatcaggaat tgctggaagc tttctaaatg
     2041 gatataaatc acattttttc ctctagcgag cccgaacaaa tcgtcatttg gtgctgcata
     2101 atgttctttt acataatttt ctacttcttt catatcattg ggacttgctt ctgcccgcac
     2161 ttggtttgtc tggtaagttt gaacaccgtc ataaaccatg aaagcattca agccaagata
     2221 tttgaccaaa taatcacgag agaatgttcg agtcagcaat tctggacgat cagcttccgc
     2281 cataaaaaga tttcctgaga aaatcataac tgctaatgta gagatcgcaa aagccattct
     2341 tgctcggatc gggcgagggt ccaacttgat tttttttgca atcagcagaa cagtcaagat
     2401 aataaaatcg atccaataga tcacatcata tgggcgaaac agtcgaatgg cactttctcc
     2461 cagaccactc gctactttcc cagcacctaa aatcgtgttg atcgtaatga agtccgtgaa
     2521 ttctcgataa taagtgacat tcgaaaaaac aagcaatgtc aaaagaaaat aaatgaccat
     2581 catcgtgata taagcaactc tagggcgtct gatataaaga gcaattccaa gtaataacaa
     2641 tgtagttgca atagggttga taaacaagat aaagtattga atgccacttt cgattccgag
     2701 atggaaatca actaaatagg caaaaatcgt tttcaaccaa agtaaaacga ctagtaaaga
     2761 aaaaaatccg agcctcttat tcaaaaaggc aggtgttttt atacatttca agataatctt
     2821 cctttctgca cttcttaatc acagaagtgt cctgaaacca tgttacacag ctcacttatt
     2881 ctcatttatt ttactagtat gacaatatag tgtcaattga aaccagaagt ttatgctgtt
     2941 tcattaaggt tttctttaag cttcatgaaa aaatgagcag taagacaagt cctatgtttt
     3001 ttgttatact tgggaagaga aagctaaagc aggtgaagga atgaaagtaa aagtaaaaaa
     3061 aagagcaagc aatcgattga aaaaaggata tccactgatc caaaaagaag accttgctca
     3121 tccccaaaaa gaaaccacgc aatgggtgga attagttgat tcgcaaaacc aatttatcgc
     3181 aaaaggatat ctaggaaagc agaataaggg aatcggttgg gtattgagcc agcagaacat
     3241 cccatttgat caatcttttt ttgaagaatt ttttgtgaag tcaaaagaaa aaagaacttt
     3301 ctttttttca gatgattcta ctaccgcttt tcgtatattc aatggagaag gagacgggat
     3361 cggcggtctg attgttgatt attatgcagg ttatgctgtt ttttcttggt ataacgaaac
     3421 gttatatgca catcgtgaat tattgattca agcttttcgt gaaatctacc cggaaatcat
     3481 aggaggctat gagaaaattc gatttgaatc agtaaatctg cctgagagcc agttcattta
     3541 cggaaaagag gcaccggaac ccttgatcgt aaaagaaaac ggagtaaaat ttgccgttta
     3601 tttaaatgaa ggattaatga ctgggatctt tttagaccaa aaagaagtac ggggatcatt
     3661 agcagaaggt ttcgcttctg ggaaaacagt tttgaacatg tttagttata ccggcgcctt
     3721 ttctgttgct gccgcaatgg gaggggcaat cgaaacaaca agtgtcgatc tagcaaaacg
     3781 cagtttaaaa aaaacacagg aacagtttga agtgaatggt ttggatgtcg atcagcaaaa
     3841 aatcattgtt atggacgtat tcgagtattt caaatacgcc aaaagaaaac aatttttata
     3901 tgacatgatt gtattggatc caccaagttt tgccagaaat aagaaaaaag tctttcgcgt
     3961 agcaaaagat tatggggaac tggtcaagga tagtctggat attttagcag aagacgggat
     4021 acttattgcg tctacaaatg ctgcgaatgt ctcgatagaa caattccaaa caatgattga
     4081 agaggaattc catgccaaaa atgtttcttt tgaacaactt gctttatatc gtctgccaaa
     4141 agatttccag acgacggata cctttttaga aggcaattat ctgaaagtgt ttgtttatca
     4201 aattaaaaat aaccattaga aggagcgatg cagaatggtt cgggtaaagg gattgaatat
     4261 aggaagcggt agaccaaaga tttgtgcgcc tcttatcggg gccgtccgag aggaagtatt
     4321 acgtgaagca gttcttgcac gtgaagcagg ggccgattta gtcgaatggc gagtcgatca
     4381 ttatcgtgaa gttttttctt ttgatgaagt agcagaaaca ttggttctta tccatgagat
     4441 gcttgatgga ctgccgttat tatttacgtt tcgtacctta gctgaaggcg gagaacaaga
     4501 catatcgatc cgtcagtatc gcgaccttta tgaacatgtg atacacacag gattagtaga
     4561 tatggtcgat atcgagttgt tcaaagtaga aagtctagga aaaagactga tagaggaaat
     4621 caagtcttta aacattcctt taattatcag tagtcatgat tttaaagaaa caccagcaga
     4681 ccctgtcttg ctttaccgat tgaatgttat ggaacatttt ggagccgata tcggaaaact
     4741 ggcagtaact cccaataatg aacgagatgt tttgcggttg atggagttga ctagacgggc
     4801 aaatgctttt ttctccatgc ctattattac gatgtccatg ggaaatcttg gtaaaatttc
     4861 tcgtctggtt ggcggaacga cgggttccgt tatgacattt ggttccttaa gcagcgattc
     4921 tacttctgct ccaggacaat taccggtcaa agcattgaag caaattattc aattattaaa
     4981 tgaaccgttg tagaacaagt ttgaggtgat attttttggc aaagaaaaaa atagtaaaaa
     5041 cgaatgcaat ccgtatggtc gaacaaaaaa agattcccta tacagagcac gaatatgagt
     5101 gggacgaaag ccacctaagt gcgtcatcgg tcgctgaaca gcttccagaa agccaatctc
     5161 gtatatttaa aacactggtt gctgtgggca atgtgacggg gcctcttgtt gctgtgatac
     5221 cgggagaagc cgaattgaat ctaaaaaaat tagcaaaagt ttcagggaat aaaaaagtag
     5281 aaatgctcca tttaaaagat ttggaagcaa cgaccggata catacgtggt ggctgctcgc
     5341 ctataggtat gaaaaaacta tttcctactt atttagatca aattgcagaa agctatgaac
     5401 aaatcattgt ctcggctggc agaagagggc tacagatgga gttagcacct caagatatct
     5461 gtgcattaac gagcggacaa tttgcagata taaaacaata aatcatacgt ataaaagaaa
     5521 gaaggaggcc gggatgttgt ttgtccagtc tccttctttc ttttttatac attatgctct
     5581 ttgcccggca ttcattcctg ctacatgccc tgtagcgaaa gccgctgtga tattaaagcc
     5641 gccagtataa ccattgacat ccagtaattc tcccgcaaaa tacagaccat caatgatttt
     5701 actctccaat gttttaggat aaatttcctt tagagaaacc cctccgccag ttacaaatga
     5761 tttctcaata ggaaaagttt tgttgattga tagcttaaac tctttgcata gttgaacaaa
     5821 atcttgaatt tgtttttgag aggcttgttg tcctgtcaga tgatccattt caagccgctc
     5881 aagaaagaaa actaacaatc gctcaggcaa tataccatgc caagcattga ccaagttctt
     5941 tttggttgct ttactttttt ctgtcaatat atgaataagc tcttgtttgg tttgtgtagg
     6001 gaaacaatcc agagaaagtg tcactggttc tcccgttttc tctaattctt tattgacaaa
     6061 acttgaacaa cgcagtgcag ccggaccaga aataccgaaa tgggtgaaaa gtaaatccat
     6121 cgtatgttca gtcaatacac gacctttttt atttaaaacg cgtaaagtga tgtcttgtaa
     6181 agaaagccct tgaagtgttt tctcctgaat atacggttca tccgatatca agggggactc
     6241 tgtcgcataa agaggcgtca ccgtgtgacc cactcgcttc acgatcttgt acccatctcc
     6301 agtagaacca gtagatggat aagtccgtcc accagttgtt aagataatgg caggagcatg
     6361 gaactcttcg aattccgtgc gtataccgta aattttatgt tctttgtgga tcaatttctc
     6421 tactcgagta gagaatagaa gcgtcacatc tagttctttc agacgattga gcaatgcttc
     6481 gataatcgtt tttgatttat ttgttacagg aaacatccgt ccatgatcct cttcttttaa
     6541 atgtacgcct tgtgattcaa agaagttcat gatatcgaaa ttattccatt gcgcaaacgt
     6601 actatataaa aactttccat ttccaggaat atgtgcaatc aaatcatcaa caggacgatt
     6661 gttcgtaaca ttgcaccgac caccacctgt catcagtagt ttttttcctg cttttttgtt
     6721 tttttcgatc aagagtacgc gtgcgccttg ttcagctgca ctgatcgctg ccatcatccc
     6781 gcttgttcca gcgccgacta cgatcacatc atacagtttt tccattccct cacctcaagg
     6841 aaagtgtagc atatctgaag aaagaaaaca gaaaaaaatg ttttgagcca aaagagaaaa
     6901 tgagctaaaa tgagggaaga taagcaaagg agcgtggaac gatgacaaac gcaacagaat
     6961 atgttcaagc aattcaagaa aagctacatc aaaaggatca agggcaagaa gaatttttac
     7021 aagcaatcga tgaattcatg cccacggtta tgtctttttt ggacactcat cccgaatata
     7081 tcgaaaagaa tatcttagaa cttttaacgg aaccagaaag agttattcaa tttcgagtgc
     7141 cttggcaaga tgatgaagga aactggcgag taaatagagg gtaccgtatc caatacaact
     7201 cagctatcgg cccttataaa ggtggattgc gcttccatcc tagtgtcaat ctaagtatct
     7261 tgaaattttt ggcttttgaa cagattttca agaatagttt aaccggtctg ccaatcggtg
     7321 gaggaaaagg cggaagtgac ttcgatccta aaggaaaatc tgataatgag atcatgcgtt
     7381 tttgtcaaag tttcatgctg gaattgtcaa aacatatcgg cccatcgatt gatgttcctg
     7441 cgggagatat cggtgtagga gctagagaaa tcggctattt gttcggcgaa tataaacgat
     7501 tgaaaaaata tgatactggt gtgttgacag gtaaaccttt ggatttttgg ggaagtaaga
     7561 tacgtacgga agctactggt tatggattag tgtattatgt caaacatcta ttgaacgaag
     7621 aaaaagattc atttaatcaa aaaacagttt ttgtttccgg aagcgggaat gtagccattt
     7681 atgcaatcga aaaagtgcaa gagctaggcg ggaaagttgt tacatgttca gattctagcg
     7741 ggtacatcta tgatccagaa ggaatagatg tgaaactatt gaaagaagtc aaagaaatca
     7801 atagacaacg tttgacggaa tacgcaaacc aacgtccttc tgctatttat catgctggag
     7861 aatctgtttg gacattgaaa gaaaaagcgg atatcgcttt gccatgtgct actcaaaatg
     7921 aaatagacga aagcttggcc gaaatcttag tagaaaatgg gatcaaaatc gtagcagaag
     7981 gcgctaatat gccatgtaca gtaaaagctg tttctgttct tcatgaagca ggcgtgtggt
     8041 attgtccagg aaaagctgct aatgctggag gagtagcagt atctgcttta gaaatgagtc
     8101 agaacgtcca gcgattgact tgggaaagag agcaagtgga tgagcagctg gatcaaatca
     8161 tggaaacgat ttaccatacg tgtcgccaaa cagcagccga gtacggcgac gaaaaaaatc
     8221 tgcttcttgg agcaaatgtt gctggatttg aaaaagttgc caaagcaatg gcagcagaag
     8281 gtttagtttg aaaaatgaaa ctttcttgga aatattgtca gcattttgtt tatttgcttg
     8341 aaaaataata aaaaaaaacg taaagtatta cctgtagagt aaacctataa ggaggagaac
     8401 aaatggcaca cattcatttt gactattcaa aagtagcacc gtttgtcaac gaacaagagt
     8461 tagggtacat gcaaagccaa gtaacagcag cccacaacga attacgggaa ggtactggcg
     8521 caggaagcga cttccgcggc tggatcgatt taccaacgaa ctatgacaaa gaagaatttg
     8581 cacgtatcaa agaagcagct aaaaaaatcc aatctgattc tgaagtatta gttgttatcg
     8641 gtatcggtgg ttcttattta ggcgctcgtg cagcgatcga cttcttgaat cacacattct
     8701 tcaatttatt agatagcaac acaagaaaag caccacaaat cttctttgcc ggaaattcaa
     8761 tcagctcaac atatattgct gacttgatcg aagtaatcgg tgatcgcgac ttttcagtaa
     8821 acgttatttc aaaatctgga acaactactg aaccagcgat tgcgttccgt gtattcaaag
     8881 agcttcttgt taagaaatat ggacaagaag aagcaaacaa acgtatctac gctacaacag
     8941 ataaagcaaa aggtgcagtt aaagtagaag cagatgcaga aggttgggaa actttcgtga
     9001 ttcctgatga catcggcgga cgtttctctg tattaactgc tgttggtttg ttaccaatcg
     9061 ctgttagtgg tgcagatatc gatgcattga tgcaaggtgc cgctgatgca agcaaagcat
     9121 actcaagcga caaactagaa gaaaacgaag cttaccaata cgcagcaatg cgcaacattc
     9181 tttaccgtaa aggcaaagta actgagttgt tgatcaacta tgaaccagga atgcaatact
     9241 tctcagaatg gtggaaacaa ttgtatggtg aatctgaagg aaaagaccaa aaaggcatct
     9301 atccttcaag cgcaaacttc tcaactgatt tgcactcttt aggacaattc atccaagaag
     9361 gacgtcgcaa tatttttgaa acagttgtga aggttgaaaa acctcgtaaa tcaatcacaa
     9421 ttcctgaaca agcagaagat ttagacggtc taggctactt gcaaggaaaa gaagtggact
     9481 tcgtaaatac aaaagcattt gaaggaacat tgcttgctca tacagacggt gacgtgccta
     9541 acttgctagt caaaattcca gaaatggatg cttatacatt gggttataca atgtacttct
     9601 ttgaaattgc tgtaggtatt tcaggttact tgaatggaat caacccattc gaccaaccag
     9661 gtgtagaagc ttacaagaaa aacatgttcg cacttctagg caaaccaggc tttgaagatc
     9721 ttgctaaaga attgaacgaa cgcctgtaag ataagggttt ttcgctagat ttaaataatc
     9781 agtgatagaa acatgttcgc tttaaaatga aaactggtac ccatttaggt actcaaacat
     9841 actgcatcgt ttattcagaa taaggggctg tgacaagacg tttgtcacag tcccttgttt
     9901 gtatagaaat cgattgattg ccttgctctt tagtttgttt taggacttta agaacagtga
     9961 ggctgacttc gaaggttaat tctgtttttt tgttttctag aatcagtaag caaaaaagca
    10021 ctcacttcga gtg
//
LOCUS       DACEPD010000088         9770 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.092, whole genome shotgun sequence.
ACCESSION   DACEPD010000088 DACEPD010000000
VERSION     DACEPD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.092"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(113..847)
                     /locus_tag="IW797_002249"
     CDS             complement(113..847)
                     /locus_tag="IW797_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ1225014.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1173..1583
                     /locus_tag="IW797_002250"
     CDS             1173..1583
                     /locus_tag="IW797_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ1225015.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1576..2277
                     /locus_tag="IW797_002251"
     CDS             1576..2277
                     /locus_tag="IW797_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ1225016.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2277..3890
                     /locus_tag="IW797_002252"
     CDS             2277..3890
                     /locus_tag="IW797_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1225017.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            3880..4101
                     /locus_tag="IW797_002253"
     CDS             3880..4101
                     /locus_tag="IW797_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ1225018.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4098..4859
                     /locus_tag="IW797_002254"
     CDS             4098..4859
                     /locus_tag="IW797_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ1225019.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5240..5749
                     /locus_tag="IW797_002255"
     CDS             5240..5749
                     /locus_tag="IW797_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ1225020.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5819..6358)
                     /locus_tag="IW797_002256"
     CDS             complement(5819..6358)
                     /locus_tag="IW797_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ1225021.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6498..7109)
                     /gene="rpsD"
                     /locus_tag="IW797_002257"
     CDS             complement(6498..7109)
                     /gene="rpsD"
                     /locus_tag="IW797_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ1225022.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7425..8222
                     /locus_tag="IW797_002258"
     CDS             7425..8222
                     /locus_tag="IW797_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ1225023.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPAATILV
                     EGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLGYEHVIANFPAFTLAYFAS
                     QGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8250..8885
                     /locus_tag="IW797_002259"
     CDS             8250..8885
                     /locus_tag="IW797_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ1225024.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8905..9678
                     /locus_tag="IW797_002260"
     CDS             8905..9678
                     /locus_tag="IW797_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1225025.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
BASE COUNT         3017 a         1753 c         2050 g         2950 t
ORIGIN      
        1 cgaagtagat aaaaagctgt tccaagctcg agttacgagt ttggaacagc ttttgtctac
       61 agtctgactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
      121 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      181 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      241 caaaacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      301 aagcaactaa tgtccataca aggaaaccga gtgctaagtg agctgtacca agattatctt
      361 ggaagaatgg tgcttttgta atacctaagg caccaaagaa taaaacgaat gaaccaaaga
      421 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      481 acacatggtt cacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      541 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      601 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      661 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      721 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      781 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      841 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaagaaacat caacacgaaa
      901 agcatcggtg cagtgtttcg tgtcggaagg atttctgcag tcttaacaaa tgtgaataaa
      961 aaaatgaatt ccattactta taagaaacca gaccaacagt aaaactccaa ctctttttaa
     1021 aagttctcca caaaccacca agcttaacta taaatatacc aaaaaaaaaa cgtttttttt
     1081 ctcaaaaatt gtatcttaga aacagcatgt tttgaaactt tcagaaaagg tttgttagta
     1141 aaatgaaagc tatacgtaag gagtgttgtt tgatgaaatt ttttcaagag tttttcatta
     1201 ttattttctt ttctgtatta ggagaaggca tacggatttt tgctcattta cctattcctg
     1261 gaagtatctt aggtattctt tttttatttt tagcatttga aatgcatctt gttgatcctg
     1321 aaaaaatcgg agcaactggt gattttttat tgaataatct agctatttta tttgttcctg
     1381 ctggtgtagg tttattggaa tattttgatg atattgcttc gatatggcct gttttactgg
     1441 gagcagtcgt cgtgtgttcc atcgtcacga tggttgcaga gggaaaaacg gcagaaggtg
     1501 tagaagcttt gctgggctat gtccgtaaga aaaaagcagg aagggtatct gtaaacgaaa
     1561 tggaggaaaa tcaaattgac tgagtttttt gtgaatccac tatttggctt aagtttgact
     1621 ttaggagtgt atgtattatc tgataaattt ttgcggaaat ataaactgcc tgttttgaat
     1681 cctctcgtat tatcgattgt tgttattatc ttatttttgc atttttctgg gatcagctat
     1741 aaagactata atatcggggg caattttttg actatgctga ttactcctgc gacagtggca
     1801 cttgccatcc ctctttataa aaacttccgt ttactgaaag agaattttta tcctgtaatg
     1861 gctgcgattt tagcaggaat agtcgcaaat tgcttggtta gtgtagggtt cggttacttc
     1921 ttctctcttc ataaagagat ggtgatctca ttactaccta aatctgtaac gacggctatt
     1981 tctgtcgatt tatctcatac gatgggcggg gtgaatgccg tgacacttgc tattgttgtc
     2041 tctacaggta tttttggttc gctgatcgcc actcatattt tccgcctatt caagatcgaa
     2101 agtccagttg ctcgcggtgt ggcgttagga agtacttctc atgctatcgg tacagcaaaa
     2161 gcaatcgaga ttggcgaaat agaaggaatc atttccggac ttgcgatatg cgtaaatggg
     2221 atattgaccg ttttgttgct gccgttattc tttcagcctt ttgctggctt gttttgatgc
     2281 gaaaaccact attttatgct acaataacca aaacgttcga gcataaaaaa ggagggaaag
     2341 acgtgtattt gtcttccgag tatcttaaac aatttgataa agatctatat tataagattc
     2401 tcctcctaga aagttttgag gatcaagctt ggcataccgc tgcacaatta gcgcaagttg
     2461 ttcagctgga tgcccgcagc gtgtcgaaat acctgaacga gctgtctaaa aactatcagc
     2521 agtttagcgg taagacacac cctttgttta caaaaaatca ccgttctggt tataactttt
     2581 atgatacgct cgattcaatt gaacatgagc gttttttgat ctatctcgtg caaagtacgc
     2641 tgaaattcca gttgctgcac gacatttttt ttgaagaatt ccatacaatg taccaatttg
     2701 cgcaaaagca ttacattagt gaatctactg ctcatcgaaa aatcaatgaa tggaaacagc
     2761 aactgcaaac ttacggaatt cggctgcaac gaggtacata tatcgctcaa ggcgaagaag
     2821 aaatcattcg actttacttg catatgacat tctggcagtt gtttcgaggg aaaatctggc
     2881 catttgaaac gatttctcaa atggacgtaa agaatatggc agaacatatc atggcttttt
     2941 ttaatgttcg tctgaacgag atcaaaaaaa gacgattgga atacatgcta ggtgcctttt
     3001 ttttaagaaa atcacagaag cattatgttg tattgaatga gaaaaaaaga cggctgattt
     3061 ctgacaatct gttgttccag cgattttgcc aagtcatgga gccggtattt cctaattatt
     3121 ttcaagtaga agacgagtta ggcgcattat ttcttgtact gatgactcgt gaagaatatt
     3181 atagtgatcc taagatacgg aagaaaattt ttgattttca tcaacaagca aaaacaccgc
     3241 cctttacagc cctttcagaa gcaaaagccg ctctttctct ttatcaggaa gagcaggggc
     3301 tgccagcaga aaatctaacc ttcgaggcag aaaactattt attcagcagt cattttttcg
     3361 cctatctttt tcctaatgcg aaagaaacga ttgatggcaa tagtagtgat tttattaatc
     3421 atttagttat tgaaaacaag gaattgaaac aatggctcgt acattttttt gaaagtagac
     3481 acaaacatcc caatcatctg gcatttaaaa atcatgcgtt tttgatgggt cggtatctga
     3541 ctgtgtttaa aacattagga gcctttactc cacagcttcc aaagatcacg atccttttaa
     3601 tgacagattt cccattattt gaagagcaac tgttagagga gggattacgg acatttttcc
     3661 gtaatgagta tcagctgatt tttctgccaa cagattatcg ggggagagaa gttgatcttc
     3721 tgatttcgac aagtaaagtc caccgcaaac catgggcgga tcttgactat tttattgtga
     3781 ccgaagaact aaaattaatt gattacatcc aactatctca aaaatttgaa atgattcaaa
     3841 aaaaaactat taaaacaata acaaagaagg agaaaaaaga tgacatttga agaaatttta
     3901 ccagaattga aaaaaggtgc aaagattatt cgtaaaggat ggagtggatt tgaactttat
     3961 gttgttctcg tatccgatga tgaatttgat ggctgtccag tgacacctta cttattgatc
     4021 aaaacaagtg acgaaggttt ttccagcttc gctcctacag tttgtgatat tttagcagat
     4081 gattgggcga tcgtagaatg aaaaaccagt atccagatct agctaaacag gttgtattta
     4141 tcacaggagc tgcttctgga attggtgctg cgcaagcccg agcgtttctt gaacaagatg
     4201 cttttgtatt tggattagat caaaagttcg gtaaaatgga agaactctct ttgcgatttc
     4261 ctgaaacttt tgcctacgca ctaggtgatg ttcgacaaat gaaagaactc caacaagcgg
     4321 tatcgaaatg ccaggatcgt tttggtgaag ttacgatttt actgaataca tcggggatat
     4381 tggatgccta caaaccattg atggaaactg acgaatcgct gtgggatctt atttatgaga
     4441 cgaatgtgaa aagcatgtat cagctgacaa aaatcatcct tcctgatatg atccgccgaa
     4501 aaagcggaac aatcatcaac atggcttcga ttgcgggttt ggttgctggc ggaggtggga
     4561 tcgcttatac aagtgcgaaa catgcaatcg tcggatttac caaacaactc gctctggatt
     4621 acgcaggaca gggtatccga gtaaaaggaa ttgctccagg agccatccaa acaccaatga
     4681 atgcggctga ttttgaaggg aaaggtgaga tggctgagtg ggtcgcaaat gaaacaccag
     4741 tcaagcgatg ggcacagccg gaagaagtag ctgagctgac gttatttctt gcctctcctc
     4801 aagcaagcta catacaagga acgatcatcc caatcgatgg cggatggttg ttaaaataac
     4861 cttgtgtttt tttcaagctt ctgtatgata aaactaaaga gagaaacaac ggttgatgat
     4921 aaaagaaagt cgatgactgt tcccgacatt cgatgccgaa cagccagttc aaaaaattct
     4981 ttttaataat cgttaatatt cgaaactaag cggcttttca cgaccttttg atataaacgg
     5041 tgttcaaaag cttgcaactg cggtaacaag tccagccaaa tgaaaaatat aaggagctat
     5101 ttttatttga ctattcttat tacttgttgc agggcagctt tttcactacc ttctgcggca
     5161 cttcagttgg aattttcttc ccatcctttg gtcgcagtca ttaccaagat aaaattatct
     5221 tgcaaaggag aaatagaaca tggaaaaaaa taatacccgt tcagccaaac gctggacaac
     5281 gacagatgtg actcggatgg cgctagtcac tggtttatat gtggcagtga cgctggtttt
     5341 gtctgtcttt agctttggag tgattcagat tcgtctctcg gagatgttca actatctgtc
     5401 cttgtataac aaaagataca tcgtcgcggt aacagcggga gtagctttgg ctaatatggc
     5461 ttctcctctt ggactgatag atgttgtagt aggaagcgtc agcacgctgc ttgtattgct
     5521 tatcaactat aaaatcacta gtcgcatcaa aaacatgaaa atcaaaatgg ctgtgacagc
     5581 atgcgtattt gctttttcca tgtttacagt agctggtcag ctaacgattt tatatcaact
     5641 accttttttc ttgaattggt gggtgattgc tttaggagaa ttggcatcaa tgattgtcgg
     5701 tgggataatt atttattgga tcggtaaaaa agtggatttg tcaaaataaa gatctgttta
     5761 atttaaaata tggcagcgtg gaagagactt tgagtcttct tccatgctgc ttttttgatt
     5821 aatttgtatg aagaaatcga tttagccgtt ttttcatcaa gataccgata taagctgctc
     5881 cagttgcctt gattgcttcc ggcagtaaaa acggcagtag tccactggca aatgcagttg
     5941 taaaagtaag atcaccactt attttcagcc agatcgttcc gcagatcaat gcagcaagag
     6001 agcccattag attggcaaag attgcatata agtaatgagc atccgttctt tcgatcatcc
     6061 aaccagttat ccacgtttgt aagagaaatc caatcaaata tccgccagtt ggcccaaata
     6121 aaacagctat acctccagtc atccctgaaa atacaggaag accaatcaag ccaagtaaaa
     6181 gataaactac aacagatatc aatcctagtt ttcttccgag aaaagttgct gttaatccaa
     6241 tagcaaatgt ttgccccgtc aaaggaacca agccaaaagg aatggtcatc tgagaaagga
     6301 cagaaataat cgcggcaaat tccgcagcaa gtattaattc tttcaatgtt cggttcataa
     6361 tgccctcttt tcgttaactt aatattttta tttggttaac gaaattgtaa aacaaagtga
     6421 ttactttgta aatagaaaat ataaaaaaag accagctatc caatagatag cggtcttttt
     6481 caagaaagct tttgaaatta taagctttgg ttgtagtatt caacgacaag agcttcgtcg
     6541 atttctggat atagttcgtc acgttctggt aaacgagtca atgaaccttc caatttttca
     6601 gcgtcaaagc ttacgaatgc tgggcgtcca acagttgctt ctacagcttc ttttactgtt
     6661 acgatgtttt gtgatttttc acgaactgag atcacttgac ccacttctac gtggtatgaa
     6721 gggatatcaa cgcgtttgcc atcaactaaa atgtgaccat ggttaactaa ttgacgtgct
     6781 tgacgacgag tcgttgcaag tcctaaacgg taaacaacat tgtctaaacg ttgttctagt
     6841 aagatcatga agttaacacc atgtttacct tctttgattt tgctagcttt tacgaacaag
     6901 ttacggaatt gacgttcgtt cattccgtac atgtgacgaa gtttttgttt ttcagttaat
     6961 tgcatgccgt attctgattg tttgccacgg ctgttaggtc catgttgacc tggtgcgtaa
     7021 gggcgacgtg ctaattcttt tcctgtgcct gacaatgaga tgcctaagcg acgagaaatt
     7081 ttccatgatg gtcctgtata acgagacatt aaaatttcct ccaatgaata taagtttttg
     7141 gagtaaaata atccgttgaa aaattcataa tcatatagtt tgttcttcaa tcttcacctt
     7201 tgcagccgcg gttacacaat cgaacccgag acggggcaac aaactaggga tgagttatta
     7261 tttttctgct gcattatttt acacaaacta cagtatatga tagatcatcc acgaatgtca
     7321 agataattag tacataaaaa cgctttacaa acctaggctg tctttataaa atgagagggc
     7381 atgtgcaaat ggaaaaaagc gaaggaagtt taaggagaaa atcaatgaat ccggtatcac
     7441 cattatttga gaaaatcgat gcttcaattg aaaagaaggc gaatcttttt gaaaacagct
     7501 tttcacgtta cgctgttcga gcggttctcg cctgtttgtt tttgacttta ggaacggctg
     7561 tggcatttgc aatcgcgatg aagggggaag atatcgcgca tggtctagga aagattttat
     7621 acgcatttat gttcagctgg ggtctggtca tgattctata catgaacgct gaattaggaa
     7681 catcgaatat gctttatatg accgtgggtg tttttcggca gagacttgct ttaagtaagg
     7741 cagccaagat tctatttgcc tgtatttttt tcaatatgat tggcggtata ttatgtggat
     7801 ttcttatttc tatgactacg ccatttcaag atttaacgtt ggacaacttt atgttagaat
     7861 caatagcagt aaaactagca aaacctgcag caacgatttt ggtggaaggg atgtttgcga
     7921 atatcgttgt caataccgct gtactgatca gcatgcggat gaaagaggat gccgggaaag
     7981 tgatcacagt cgtattcatc attttcattt ttgctttttt aggatatgaa cacgtgatag
     8041 cgaattttcc tgcctttacg cttgcttact ttgcttctca aggacagacg gatggaatga
     8101 cagtcagcaa tgtgttacat aatttgtttt ttgctttggc aggaaattat attggtggcg
     8161 gtttagtaat gggactggga tatgcttggc tgaatcagtc aaaatcatcc tatgtggact
     8221 aaaaagtcgt tcattaaagg ggaagtgcca tggaagtttt agtttttcca aaaaatgagg
     8281 aaaaagcgtt acaaatggaa aagtacatga aaaatctttt ttcctttgct ggagtaccta
     8341 agccggaacg cgctctttta caacaaccgt tactggatga gagcaaaaat ttatctattt
     8401 ctgaagtttt agcttttgtt tattattatt atgaacaaat cgaaagagag tatcagtatg
     8461 tagcgatcga tttagtccag aaaaacatca aacggcttac atttgaagac ttagtcgtat
     8521 tgttgccact agttcagaaa aaagaatggt gggacagcat cgactcttgg agaaaagtat
     8581 acagcacatg gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa ttgtttgaaa
     8641 aaaaggaaga tttttggcta cgtcgaatag caatcacgtt gcaattaggt tataaacagc
     8701 aaacagacac aggactattg atccgagcaa tagacgaaga tcgtcaaaca aaggaattct
     8761 ttatccaaaa agcaattggt tgggcattac gcgattacag taagaccgat gccgattggg
     8821 taaaaagaca gctgacaaca gatttgagta agttagctgt tcgagaggca agtaagtatt
     8881 tataagaaag cgagacgaca agagatgaga cgcaaattat ttgcttttga tattgatgga
     8941 acgttgttag attcaaacaa acaagcttta gatagcacga gagaagcatt ggaaaaatta
     9001 cgcaaagccg gtcatctggt cacgattgct acagggcgga gccgatttca tgcgcaagag
     9061 atcattcgcg atcttgattt tacgaactac attttatgta atggagcggc cggtttttta
     9121 gaccatgaac aagtatataa gaatttacta gatcgcgacc agttggatgc gttcgttcaa
     9181 gaagcgcatc atgagcagat cgatacagct tttgtcagct tagacaatat caagcgttct
     9241 tcttccaatg atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc tgtcttgcct
     9301 gaattagatc agttttttgc agaggaacaa gaaatatacc aagcgttagc atttttcgat
     9361 caaacatatg atgggaagtt ctcttcatat gataaaattc gatttgtccg ctggcatgag
     9421 aacagtgtag atgtcgtacc taaaaatggt tctaaagcag cgacaattct gcacttagca
     9481 gaacaagtag gcatccctca tgaagacatc atcagctttg gcgatggaca aaacgaccgt
     9541 gaaatgttgc gcatgtcagg aattgggata gctatgggga atgctgtgcc agaagtacaa
     9601 gcagaagcta agatggtcac tgatacaaac gatcaggatg gaatttggaa ggcattgaaa
     9661 gagttaagcg tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag taataagccc
     9721 agccaaacaa aaatagctcc tcctattttt cgtttttctg ggcttattac
//
LOCUS       DACEPD010000089         9155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.093, whole genome shotgun sequence.
ACCESSION   DACEPD010000089 DACEPD010000000
VERSION     DACEPD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.093"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            807..1007
                     /locus_tag="IW797_002261"
                     /pseudo
     CDS             807..1007
                     /locus_tag="IW797_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1494..1673
                     /locus_tag="IW797_002262"
     CDS             1494..1673
                     /locus_tag="IW797_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225026.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1719..2324)
                     /locus_tag="IW797_002263"
     CDS             complement(1719..2324)
                     /locus_tag="IW797_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1225027.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2506..2841
                     /locus_tag="IW797_002264"
                     /pseudo
     CDS             2506..2841
                     /locus_tag="IW797_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3006..3233
                     /locus_tag="IW797_002265"
                     /pseudo
     CDS             3006..3233
                     /locus_tag="IW797_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3359..4330
                     /locus_tag="IW797_002266"
     CDS             3359..4330
                     /locus_tag="IW797_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1225028.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICKENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4470..5783
                     /locus_tag="IW797_002267"
     CDS             4470..5783
                     /locus_tag="IW797_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ1225029.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5826..6692
                     /locus_tag="IW797_002268"
     CDS             5826..6692
                     /locus_tag="IW797_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1225030.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLMPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6689..7537
                     /locus_tag="IW797_002269"
     CDS             6689..7537
                     /locus_tag="IW797_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1225031.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7551..9023
                     /locus_tag="IW797_002270"
     CDS             7551..9023
                     /locus_tag="IW797_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ1225032.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9040..>9155
                     /locus_tag="IW797_002271"
     CDS             9040..>9155
                     /locus_tag="IW797_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ1225033.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEET"
BASE COUNT         2920 a         1526 c         1871 g         2838 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc atttaaaaag aaaagtgaca
      361 gataggaata aaaacttatt aaatgtagtt atgatctgtt atatttcatt tctaatacta
      421 ggtcttccac aagcagtgtt tcctgaaatg ttttctggag actggggtat tgtcagctat
      481 tctatattaa cattgattgc tatggtttta ggtacttcat tttataatgc ttttgtaagt
      541 aagcatgttg aggaatatga agtattaatg tacttaaaag agaaaaaaac aagtaattaa
      601 tatgagaaca ccacgcatag tttttatttt gagatattct agaatgaaac acattaagaa
      661 cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc taagtttttt catatttgtt
      721 tgttatgcta tacatagatc tgcataatta gacttgcgct gcccgttatt gaagtttcag
      781 gcagcgctct ttttggggga tcgataatgg aagaagaaaa aatatttgag aaacgctggc
      841 agctagcatc aagtgaacaa agagcaagat acaataattt gatcacctcc taccccactg
      901 ttgactagac atataaggaa aagaaatact tactttggct gtgccagtta gatatagata
      961 cctttgaaac atttgaagtg atcctagata aaattaaaca gaattgatca gaaacaagcg
     1021 aagccttctc tcggttcgct tgttttgcta tttattagtt ggctcagggc aactctaagc
     1081 tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta ccactttaga atacatggat
     1141 gtacgtattg ccattgactc ctattagtta ctgaaaaaaa atttaaacaa gttgagtatt
     1201 gcagataaat agctccagtg aggtgaataa ctgctccact caaggcaata gatagctcca
     1261 tccagggcaa caaactgctc cactttcata tttaatagat gaataacctt ctgtataata
     1321 gaaaggcata cctttcggta tgacaactta tacagaatgt tattttattg gttcactatc
     1381 gaaaaatttt ggagctttat ttttccggta tttctcaacg aacgattagt ttaactgtgg
     1441 gaagctccag acatacgatt cgagaagtta cttctcgagc agacaggaag ggggtgactg
     1501 aactaaccgc tgatatgagc gatacttggc tgcaggaata tctttttccg gaaaaacaaa
     1561 caacagcacg aggttacctt cctgtagact gggaatatgt tcataaagaa ctaatgaaaa
     1621 agaatatgac attgaaactc cttcatagag aatatggggt acatggaaca tagaaacgaa
     1681 aatgcacggt agaaaagcga atgaatatag tataaattct acaaatcatt cgctttcttt
     1741 attttatata tggttggtcg agagagacca ttttctttgg caatttctgc aactggaact
     1801 ccttgattta acatgtccac cacagtttta aagactaatc gtttttgcgg atctgttgag
     1861 ttctcagaat attcaattgg tcgcccctta tatcgaccag cttttttggc caataatact
     1921 ccttgccgct gacgttcacg gattttctca cgttcgtttt gtgcaatata acttagcaat
     1981 gataaaaaca tatcgaacat agcagtatct aggagttcat ttccagtatt aaaatctaaa
     2041 aactgtgcat ctagtatttt taatgctact tttttctgtt tcataaaggt aactgtattt
     2101 tttatgtctt cataatctct gcctaggcga tccagtgatg caacaatgac acaatcacct
     2161 tctcggatat aatttagtaa tttttgaaac tcaggtcgct ggatatcttt accactggct
     2221 ttttcttgga ataatttatc aatgttaagg tcttccatca ttgccaattg tcgatctaaa
     2281 ttttgatctt gtgaagacac tctaatatat ccaatttttg ccataaaaac tccttttaaa
     2341 aaatgatata atttacatgt aaaatttact tgtagtttta ctgttatcat agcattttta
     2401 aaaataaatt ttaagtctac ttataattta ctttggaggt tttgaatgaa tgaaattaag
     2461 cgcaataaaa attcttttgg tgtttattta ggagagccta gctcggaaca gttaaataaa
     2521 ggtgaagctc gtcatgctct atgtcgaaat atattttatg gtagaaaagg caaactttat
     2581 caaacttatt acgacggtat ggaggaacag ctcaacgcat taggattagt aactaatgct
     2641 atcatatatt ggaattcact atatttagaa aaagtctttg aacaaattaa aacggaaggg
     2701 ttcgactgtt ccgaagaagt gttacgcaag caatttccat taattagtga tcacattaat
     2761 tttgttggca aatatagttt ccgctatgaa aaagaattat cgaatggaat actacgtccg
     2821 ttaaatatag agaattattg agaatgttcg tcttttggtt cctaccgtgc attttcgcac
     2881 ggatgttcca gatactattt ttgaatgtat aaaattgaaa acacaagaat actaataaat
     2941 caacgattct tgtgttttta atttttcact accgtgcatt ttcgtttcta tgttccatgt
     3001 accccaatct tgaaaaatta attaaagact tagataaagc cattgacgac ttagtcgtgg
     3061 tgctaccgga gtatcaatgt ttaaccagta tcccaggtat tggtcctgtt taggctgctg
     3121 gaatactcgc tgaaattggt ccaattgaac gtttttctga tgaaataaaa ttgacaaagt
     3181 atgccggcct atattggcga cagagtcaat ccggtaaatc tgaatacgaa aattttttta
     3241 cgaaaagctt cacgtctaat atctatttta aaataaaaat attcctgcta atttggaatc
     3301 gtttccttct tttggtataa taatacctga aattcttttg agaggagttg ttcgtgaagt
     3361 gaaaactact atgaaggatg ttgctgcgtt agcaggagtt ggtgtgggta cggtatctag
     3421 agtaataaat ggagtaaagg tgaaagaggt taccaaagaa aaagtaatgg ctgcaattag
     3481 tgagcttaat tatgagcctg atgaatatgc cagaggtatg aaaacgaata gtagcaatac
     3541 aatagctctt attttaccta cgatttggca tcccttcttt tccgagtttg cctactatgt
     3601 agaggaagct ctcagccaaa aaaattataa gctttttttg tgtaatgcga acggcagtgc
     3661 ggaaaaagaa aaagaatata tccaaatggt caagcagagt aaagtagacg gaattatagg
     3721 tattacctat tcagacattg ataaatatgt atcatcaaat ttaccatttg tcagtataga
     3781 ccgccatttt tcggaacagg tttcttatgt aacggcagat aattatttag gtgggcaaat
     3841 tgccgctcaa gaactactta ctcgtaactg taaaaacttg tgctatgttg ggggtgtttc
     3901 accttaccct aacgaaacaa acaatcgtaa agctggtttt tacaatattt gtaaggaaaa
     3961 taaaatagct gtgaaggtat tggatatgcc tgaaccaatt agcaatttag aggaacaatt
     4021 gctcgctttt ttttcggata atcctgaaat tgatagcatt tttaccatca atgatttcat
     4081 ggctttggat gtgataaaag cccaaaaaaa actagggaag gaagcaccta aggattttca
     4141 aattattggt tttgatggag tcaagctatc gttgcaaaaa gactatttac tatcaacgat
     4201 tgtacagccg atagaggaaa tggccaaggt ttcagtagaa acactcatgg cgatgatttt
     4261 aaatgaagaa ccgcctaagc gtgtggtagt accggttttc tttcacgctg gagagacgac
     4321 taaagagtaa aaaaccggtt tataacgatt tttttgagat aggttgacag aaagtgattt
     4381 caatgatatc ttaaaaccaa ttaattggaa acgttaccaa ttaattgaaa aataaaacgg
     4441 aaacgtttca aaaaatagga ggaacatcga tgaaaaagag aatgttagca attgcagcaa
     4501 ttttagcatt aacagctgga ggacttgcag gatgtggcag taaggagaaa agttcaggta
     4561 gcagtgatcc aggagaaatt aagatttggg ttcagttttc tgatgaaacg cctgaaggaa
     4621 aagcttgggc agagattgtg aacaatttta atgatgaata cgagggtgaa tataaagctg
     4681 ttactgagta tattcctcgt agcggaagcg gcggtggata tgaggataaa gtaaatgctg
     4741 caattacaac caataccctt cctgatgtta ttacgttaga tggaccgaat actgcagctt
     4801 atgctcattc aggagtcatt gctccattag atgattattt gaaagatgcg gacatggatg
     4861 atgtattaga cagcattatt cagcaaggaa cctacgatgg caaaatgtat gcgtttggtt
     4921 tctcagagtc cagtgtgggt gtctattaca ataagtcgct gttcaaagat gctggaatta
     4981 cagaagatca attgccgacg ttagaagatc cttggacttg gacggaattt caagcaattt
     5041 gccaaaagct gactgataaa tacaaaacgc ctgcaattga tatgcaaatg gcgtcgggag
     5101 acgaaatgct gacctacgca tatacaccat ttatatggtc gaatggcggc gatgtcgtta
     5161 atgctgatgg aaccaaagcc gaagactatt ttaacggtaa aaaatccgtt gaagcgttgg
     5221 aatttattca agaattggtt gaaaaaggtt acaccacaat cagtcctatt gaaaaaggat
     5281 ttgaaactgg caaatatgcc atgatgctga gcggttcttg gacaatggca gatttagagg
     5341 aaaactatca agagattgat tatggtattt tgccttaccc cgtttcagat gcaactgggg
     5401 agttagtttc accaaccgga agctggcagt tagcaatgac tgagaaaaca gagaaaaagg
     5461 atgcttctgc ggcctttatt aagtttgcaa ccagtacaga atctagtcgt attatgagtt
     5521 tagggaacag tgttctacct atcagaaatt caacagttga tttaattaaa gatgaagtgt
     5581 cggaaggaat gcaggtatta attaagcaaa accaaatttc tggacatgcc cgcccagtgg
     5641 ttgtagctta tccgcaagta tctagagcat tccaacaagc agttcaagat attagctact
     5701 tcgaggaaaa cccagatgta caaaaagtag cagatagtcg cgctgctgaa atgcaacagg
     5761 caattgatac ttcactaaaa tagaagtttc taaagggggc caatcaagtg ttaaaaaaac
     5821 cgacaatgaa agacaatttg ataggttacg gctttctagc gccagctttg ctgctgctga
     5881 cgatttttct gatcataccc gtaatcatgg taatttatta tgcctttact gattattatt
     5941 tgttaatgcc agatgctaga caatttatcg gcttagaaaa ttttaagcga ctgttcgctg
     6001 atccaatttt tgtaaaaagt atttggaaca cggctaaatt tgttgtttgg attattccgg
     6061 tccaaatagg ggcagcttta ggaatggcac ttattatcaa caaaaatcgt aaaggaaata
     6121 tcttttttaa agtagccttt tttgcgccag tcgtgatgtc cttagtcgtt atttcgattt
     6181 tatggttgta cttgttaaat cctaatgaag gtctggttaa tacgctattt gcaaagattg
     6241 gaattgcttc gcagcctttt ttgactagtc ccaaacaagc aatgtatacg cttgtgttcg
     6301 tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt tttaggggga atgcaaaata
     6361 ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt ttctaagttt cagcaatttc
     6421 gctatatcac catgccatta ttaaagccca cggcgatttt tattttactt accacattga
     6481 tttcggcctt taaactaatc attcagccaa tggtaatgac tcaaggtgga ccaatgaatt
     6541 ctactatgac aatggtttat tacatttacc aaactggatt tacagaccgt atggtaggct
     6601 actccagctc cattgcgtta atctttacca ctatgattgg tttaattaca attgctcaaa
     6661 gaaaattggt aaaggaggat gatccagcat gaccaagaaa tttggaccgc taatgatcgt
     6721 agaatattgt ttgctgttcc ttttagcggc attattcatt ttcccgatgc tgtggatggt
     6781 agtttcctct atgaagcctg aagcagatgt ctatcaaaat ttagcttcct tcaaagcttt
     6841 tttacctagt ttgaatccag ctaattggtt taaaacttat caagaggtta tttcccggtt
     6901 cagtgtggga acttatttat taaacagtat tttttatggt ttaacctttg cctgtgggtc
     6961 aatcattgta aactctttgg cgggttttgc ctttgcgaag gttaattttt caggcaagaa
     7021 aattatcttt ggagctttgt tagctctgct gattgtcccg atggagacgg ttttaattcc
     7081 gcaatttacc atcgttaatt cactaggctt agtgaatact cgcttggcag tgattttacc
     7141 agcgatggcc agcgcgttta atatctatct gtttagaaat ttctttatcg cgattccaga
     7201 agaaatcatt gaatcagcga aattagatgg tgcaaatatt gttaaaattt tctttcacat
     7261 catgctgcca atgtctaaac cagccatagc cacagtaggt gtcttatcct ttatctccag
     7321 ctggaatgat tatatttggc cgttaatggt cttaactgac agcagcaaat tttcaatgca
     7381 agtggcaatt actacgatca atactacaca gccagtttat atcaatcaag tgatggctgt
     7441 tttaaccatt gcaacgattc cactgattat tgtttatatc gttgcacaaa aatatattct
     7501 gcagggactt ggcggttctg gaacgggtat aaaataggag tgaatagtga atgttgcaaa
     7561 aacttttaaa ggccaatgag tttattgaga aaaatgctca aacagtcaat caagaatacc
     7621 ggaataaatt tcatttgatg gctccgattg gctggatgaa tgatccaaat ggttttgttt
     7681 attatcaaga ggagtatcac ttgttttatc aatattatcc ttacgacagc atctggggac
     7741 caatgcattg gggccatgct aaaagcaagg atctaattca ctgggagcat ttgccagtag
     7801 cactggcacc aggtgaaagc tatgacaagg aaggctgttt ttccggcagt gccatagaaa
     7861 aggatggcaa gctgtatttg atttatacag gacacaatgt tgtggaaggg caagttcgac
     7921 aagttcagtg tttggcagtt tcagaagatg gcattcactt tgaaaaatat gccggaaatc
     7981 caatcatcgc agaagatcac ttagcttctg ttgcaacgac cgaagatttc cgcgatccca
     8041 aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc tactaaaact gcagatgctc
     8101 gtggacaaat tgtgatgttc cattccaaag atttactaac ttgggagttt tattccgtat
     8161 tgctggaggg aactccagaa caaggcattg tatgggaatg cccagacttg tttgaactag
     8221 atggcaaaga agtcttgatt ctttctccta ttcaaatgac tccacaaggc aatgcttacc
     8281 aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga ttggcaaaca ggcaaatttc
     8341 tagtagagaa ttttcatgaa atcgatggcg gtttagattt ttatgcaccc cagacctgtg
     8401 aaaacgaagc aggccaaaga ataatgattg cttggatgca aatgtggcag cgtaaaatac
     8461 ctacgaatga tctgggccat ggttggtcag gttccatgac cttgcctaga gagctgaggg
     8521 tggctgataa tcaattgttg caacggccag tttcatcaat ttatcaagta attgatccgc
     8581 aggaaaggat tgaatgggcg aagactgaag agaatcgctt cttactgaag gacttagtat
     8641 atgaccagca ctatttacgc ttggaaatca atttaaaaca ggcaacgaaa ctagaacttc
     8701 actatgcgaa gggacccgac catacattac tgcttaacta tgatgttgaa cgggagtgct
     8761 ttactgcttc cagggaacac atgccgtatg atttatcagg agctgaaaag gaagtgttga
     8821 aaaagcgcag tgtgacgatt cctttggaaa ataattgtct aaccatcgaa attttccgtg
     8881 atacttcagc tattgagctt tttttaaatg atcgtctgac tatgagtaca actttttatg
     8941 agacagaaaa aggacaggat tttcttttgc tggcagacag tgaagtaacc ttttcattgg
     9001 aaagcggctt aattaatcta tagaagggag agctcagtga tgatttatgg ttcaatcgaa
     9061 gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag aaatgacgat caaggaaaga
     9121 gtgagctttc cgacaacgac acctgaggaa acaat
//
LOCUS       DACEPD010000090         9129 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.094, whole genome shotgun sequence.
ACCESSION   DACEPD010000090 DACEPD010000000
VERSION     DACEPD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.094"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            38..1225
                     /locus_tag="IW797_002272"
     CDS             38..1225
                     /locus_tag="IW797_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HAQ1225034.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            1206..2510
                     /locus_tag="IW797_002273"
     CDS             1206..2510
                     /locus_tag="IW797_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ1225035.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            2536..3549
                     /gene="citC"
                     /locus_tag="IW797_002274"
     CDS             2536..3549
                     /gene="citC"
                     /locus_tag="IW797_002274"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAQ1225036.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            3553..3846
                     /gene="citD"
                     /locus_tag="IW797_002275"
     CDS             3553..3846
                     /gene="citD"
                     /locus_tag="IW797_002275"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAQ1225037.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            3851..4756
                     /gene="citE"
                     /locus_tag="IW797_002276"
     CDS             3851..4756
                     /gene="citE"
                     /locus_tag="IW797_002276"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAQ1225038.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            4749..6308
                     /gene="citF"
                     /locus_tag="IW797_002277"
     CDS             4749..6308
                     /gene="citF"
                     /locus_tag="IW797_002277"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAQ1225039.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            6305..6862
                     /gene="citX"
                     /locus_tag="IW797_002278"
     CDS             6305..6862
                     /gene="citX"
                     /locus_tag="IW797_002278"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAQ1225040.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            6855..7718
                     /gene="citG"
                     /locus_tag="IW797_002279"
     CDS             6855..7718
                     /gene="citG"
                     /locus_tag="IW797_002279"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAQ1225041.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            7952..8361
                     /gene="tnpA"
                     /locus_tag="IW797_002280"
                     /pseudo
     CDS             7952..8361
                     /gene="tnpA"
                     /locus_tag="IW797_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(8998..>9129)
                     /locus_tag="IW797_002281"
     CDS             complement(8998..>9129)
                     /locus_tag="IW797_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1225042.1"
                     /translation="RRREKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         3182 a         1420 c         1852 g         2675 t
ORIGIN      
        1 ggttttgtaa ttaaaaaatg taaaaaggag tgaaggtatg cttgatgaag ttttagaaat
       61 ccataaaaaa aatgtaggtg ttttacggat ggaaacaaca gaagccgtgc aagatgatca
      121 agatttagca aaaatgtaca ccccaggtgt agcagaacta agtttaatga ttgagaaaaa
      181 tcatgctttg gcaagagaat taacaattag tggtaaatta gtggccgtta ttacagacgg
      241 gtcggcggta ttaggtcttg gaaatgtagg aaaccaagct ggattaccaa tcgtcgaagg
      301 gaaagcgctg ctttataaaa atttagcagg tgtcaatgcg attccattag ccatcgaaca
      361 aaaatcagta gacgagattg tacaaacaat tgtcaatttg caaaatagtt ttgcagggat
      421 ccatttagaa gatattgcag caccgaagtg ttttgagatt gaagaaaaat tacaagaaaa
      481 actttctatt cctgtgtatc atgacgatca agaaggaaca gcgatagttg ttttagctgg
      541 attgatcaat gctgctaaga tccaaaggaa aacattaact gaacttcgag ttttgatcaa
      601 cggcatggga gcatctgggg ttgcaacagc tagactatta atcgctgctg gtatcaaaaa
      661 tctaacattg gtggataaac aagggagatt aaagaagcaa gatagtacat taaataaata
      721 tcaaagagag ttgttggatc atatacatga tacaaataat aatgcaagcg ttttggaaga
      781 tgcaatcgtt ggacaagatg tttttatcgg tctttcaata ggaaatgttt taacgcctga
      841 aatgattgga aaaatgaatg ataatccgat tatttttgct ttagccaatc cagtaccaga
      901 gatcacaccc gatttagcaa aagaatctgg tgcaaagcta atcgctacag gttcttcgaa
      961 atatcctaac caagtaaaca atattttagt atttcccgga ttgttcaagg gactattact
     1021 atcgaaagcg aataaagtcg atctttcatt acaacttgaa gtagccaaac aattagctgc
     1081 taaggtcgca gagcctacgg tagaaaaatt catccctaat gtttttgatg aaggagtcgc
     1141 aaacgtcgtt tcacaggcag ttttggatta tttacaaaaa ctaaaagaaa acgaggataa
     1201 tatacatgct gaaaacatta aatagaatta aaattggttt tattccttta tctctttata
     1261 tcattttggt actcgcgtat cttgcaatga cgataagcaa tatcgttcca gaaaatgata
     1321 tgttagtttc tttgggtatt atgactgttt ttgcttattt attagaagaa attggtaaaa
     1381 ctattcctat tttaaaagat ttaggtggta aagtattagt tgttacattt ttgcctgcat
     1441 ttctagtcta taaacattgg ctacctcaaa gtactgtaac tgtggtttct gattttatga
     1501 ataataacaa tttcttgatg ttctttatca cactactagt tgtcggcagt attgcttcaa
     1561 tgaatcggca aacattggtt aaagcaactt cacgtattat cattatttta attttatgcg
     1621 atgtgattgg ttctttgata ggaacaggtg tgggaatact ccttgggcta tctgcttgga
     1681 atgcgtattt ctttatcgtt gcgccgatta tggctggtgg tgttggggaa ggagcgatgc
     1741 cattatcgat tggttatgca gcaatattat ctggtatggg acaaaatgat atctttggga
     1801 agattctacc gtgtgtgatg ttgggaagtt tagtcgcagt tctactgtct ggtatgctca
     1861 aaaaaattgg agagaaatac ccacaattaa atggtcaggg taatttgatc gatggggatg
     1921 atgaacattt agagaacttg aagggttcag ctaaaccagt caatattgaa aaaacattgt
     1981 tagcaggcgt ggtagcaata acgatttatt ttgttgctgt ctatgttgag cacttattaa
     2041 aatcactcct tggcttttct attccttcac ctgtgatctt attagtctct cttatggggg
     2101 ctaaaatgct tggttttatc cctaaaaata tcgaagaagg tggaaattcg ttgtatggat
     2161 tcactgttcg tgggatcaca cctcccttac tttttggcgt aggggttgca aaaacagatt
     2221 ggttagagct gatcgaagta tttaaaaatc ctgcaacgat agtaacaatc attgtgacgg
     2281 tagtagctat tgtcgctaca gcttttatcg ccgcaaaatt tttaaaaatg tacccagttg
     2341 atactgccat cgcagtttca tgttgtagcg gacaaggcgg tacaggtgct ttagctatct
     2401 tagctgcggg tgatcgcatg gaattaatgc catttgcaca agttgctgtt cgactagggg
     2461 gtgctatgac cgtaacgttc gctatctttt taatgggtct gctatcataa gaacataaag
     2521 aaaggacgtg accatgtgtt tgatcaaaca gatatccaac gattattttt aaaaaataac
     2581 tatatcaaag accaatggga aaatcttttg cttgattcag gaatacaaaa gaagcaaatc
     2641 gaagattttc aacatttaga tcaaacattg ggtatatacc ataaagaaaa gttagtagga
     2701 acagtatctt atcaaaacaa cgtgattaaa tatatcgccg tgtctgaaaa atataaagat
     2761 ggtggacata catttaatgt actagtctct gcaataattc aggcaatgag tcaacaagag
     2821 atttttcatt atattgtttt cacgaaaccg cgctatcaaa aaagttttaa ctttttaggt
     2881 tttgaaacaa tcgtatctac cccaaaaata gctgtattag aaatgggaga tagttctata
     2941 aaacagtacc tagagaggat tcctaaagtg aatggtgaaa aaaaagcagc aattgtcatg
     3001 aatgcgaacc catttacaga agggcattat tatttagtaa aacaagctgc caataacaac
     3061 gattgggtat atgtatttgt actcgaaaaa gaacaaaatc ttttttcgac gaaggaccga
     3121 ctcaaattag ttcaacaagg attacaaaaa ctgaaaaatg tcattgttct atcaggtggc
     3181 gagtatatta ttagccctgc aacgtttccc acctattttt tacgagaaga tgatgaaata
     3241 gcaaaggagc aaatggtcgt agatgctact ttattcaaag aaagaattgc cacttattta
     3301 gatatctctg ctagatatgt gggagaagaa ccactttccc caatgactcg ttcatataac
     3361 gacatactgg aaacgatttt accccctgaa ataaaagttc atgtccttgc aaggaagaaa
     3421 acggaacaac accaagttat ttctgcttct caagttcgta aagcgtatct tgctgggcaa
     3481 ttggaaaaaa taaaatatat ggttccagaa acaacctatc aatacttaaa aaataaaagg
     3541 gagcgttaag taatggaaat aaataaatca gcgattgctg gaacattaga gtcaagtgat
     3601 gtccaaatca tgatttcacc gaatacagaa ggaatagaaa tcgatttaga cagtcaagtg
     3661 attgagcaat ttggaaaaca aatcaaaaaa gttatctatg acacactagc agtttatcag
     3721 attaaaaacg taaaactcaa ggtagtagat aaaggggcat tagattgcgt tattgttgca
     3781 cgattaaaaa cagcgatcca tcgttcctta ggtatccaac accaagaaac accgtgggag
     3841 gtttaattag atgatgaaag aacgtttgag aagaacaatg atgtttgttc caggaaataa
     3901 tccagcgatg atcaaagatg caggtattta tggtgctgac tcgattatgt tcgacctgga
     3961 agatgcagtg tctatggcag aaaaagacag cgcaagaatg cttgtggctg aggcattgaa
     4021 aacagtggat tatggtgaca cagaattaat tgttcgaata aatgacctgc actcagaatt
     4081 tggacgtgca gacatttttg caatggttaa agccggcgcg catgtattac gtttacctaa
     4141 aacagaaacc tctgaagata taacagaagt agaaaaagtg attatagaag cagaaaacta
     4201 tcataatgtt ccgatagggc gaacattgat gatggcagcc attgaaggtg cttctggtgt
     4261 attgaacgca cctgcgatcg ccaaagcatc tgatcgattg attggaatcg ccattggggc
     4321 agaagactat gtgacaaata tgaaaaccaa acgatatcca gatggaaacg aattgttttt
     4381 tgctcgcagt atgatcgttc atgctgcacg tgcagcaggc atttccgcat ttgacactgt
     4441 ttactctgat gtggatgata tggaaggatt tttaaacgaa gtacgtttaa tccatcaatt
     4501 aggttttgat ggaaaatctt gtatcaatcc acggcaaatt ccagtagtca atactgttta
     4561 tcagccgtca atcaaagaaa tcaaacgatc actagacatc atctcggcaa tcgaagaagc
     4621 aaaagaaaaa ggttctggcg taatttcttt aaatggaaaa atgatcgata aaccaattgt
     4681 agaacgtgca caacggacat tgatgttagc aaaagcatca cgattgattg atgaggaggg
     4741 aaatatccat gcataataaa caaagacgta gcattccatc aagtgtttta acaaagtata
     4801 aaaaagaatt ccaaagtgtt tcattatcgc cacaaacgat tcaacgttgt gcaccaaaag
     4861 taacgactgt aactaatcag acaaataaat tgatgcactc gatcaaagaa gctatagaaa
     4921 aatcgggctt aaaagatgga atgacgattt catttcatca tcatttgaga gaaggtgatt
     4981 ttgtttttaa ccaagtgatg gccgtgatcc gtgaaatggg catcaaagaa ttgaccttag
     5041 caccaagttc tttaacgaac actatgaatg agatggttat tgaatgcatc aaggacggta
     5101 cagtaactca tatcacaagc agtgggatgc gcggcagctt aggtgaattt atttctcatg
     5161 gaggaatgga caatcctgtg attttacgtt ctcatggtaa tcgtccacgt gcaattgaaa
     5221 gtggtgaatt gaaaatcgat gttgcatttc taggagtacc aacatctgat gattatggaa
     5281 atgctaatgc gactgttgga aaatccgttt ttggttcatt agggtatgca aaagtagatg
     5341 cacaatatgc agataaagtc attcttctaa ctgatcattt agttccttac cctaatacgc
     5401 ccattagtat tccgcaaaca caagtcgact atgtcgtgga agtagaagca attggtgatc
     5461 cgaataaaat cggtgctggt gctacacggt ttactaaaaa cccaaaagaa ttaaaaattg
     5521 ctgaaatggt caaagatgtc attgttcact caccttattt caaagatggc ttttcttttc
     5581 aaacaggaac gggtggtgca gctttagcag taacgcgtta cttagctagt gaaatggaag
     5641 aaaaacagat caaagcttct tttgcattag gtggcatcac taaaccaacg gttgatttat
     5701 tggaaaaagg tcttgttgaa aaagtgttag atgtccaaga ctttgacaaa ggagcagctg
     5761 aaggaatggc gcggcttgag aaccaacagg agatcgatgc ttccttttat gctgatccaa
     5821 gtaataaagg agcggttgtt aaccaattag atgtcgctat tttatctgca ttagaaattg
     5881 acacaaactt caatgtaaat gttatgactg gttcagatgg tgtgctaaga ggtgcgattg
     5941 gtggacatca agatgcagca acggctaaac ttactatcat ctcagcgccg ttagtacgtg
     6001 gccgaatacc tacagtagta aatgatgtta ctacattgat cacaccaggg aaaagtatcg
     6061 atgtattagt cacagaagta ggggttgcta tcaatcccca acgtaaagat ctaatagcta
     6121 tttttgaaag aattccacaa attcctgttt ttacaataga agaattacag caaaaagcag
     6181 aaaaaatagt aggcatcccg gaacctttgc aattcacaga tcggatagtt gcctatgtag
     6241 aataccgaga tggttcaatt ttagatgtcg ttcgtcaggt gaaagaattt gaagaagaga
     6301 gatcatgatg tttgatactg gagaaattca agaaatcgag gatattttga tccataaaga
     6361 acaacgtgct aacttgcaaa gcaacctgct ttccaagtat tcaaatagtt cattgatggt
     6421 ctttacattg aatattcctg ggccgatcaa aaataatcca atgattgagc aggtatttcg
     6481 tcagggacag cagtcaattc agcaaatgtt tttaaaaaaa aaggtggaaa tgacgtacac
     6541 aaaagaaatc aatttgccaa gtggattgga ctggtacgta atagtaacag aagcaccttt
     6601 gaatctcaaa aaatggttgg tcacgttaga agagcaagac gcattaggta gactttttga
     6661 tttagatgtc ttgttcaaaa aaggagagca gataacaatt gtcagtcgcc aagctcttaa
     6721 tcatggggaa cgtccttgtt ttatttgtgg gaaatcagca aaggtatgtg cacgtaatcg
     6781 tacacatact gtgaaagaaa tgcaaaagaa aatcgaacaa atagtagccg attccttaaa
     6841 aggagcattg aaacatgcat gataatattc aaaaaatagc acaagaagca caattagcga
     6901 tgctttatga agttacatgt atcaataaac caggattggt tgatccagta gaccccgggt
     6961 ctcatcaaga tatggacatt tttacttttc tacaaagtag tgtagttttg actccctatt
     7021 ttgaagaatt tatccaaaca ggattggacc gacaacatca accaattgaa gaaaccttta
     7081 tggcgattcg acagattggt ttagaggctg aaactgcgat gttctcagtg acaaatggta
     7141 taaatacaca taaaggtgct atcttttcat tggggatttt tcttgctatt tgtggccggt
     7201 taactatttg gaaagttcct tgtaaaaaaa gtttctttca aaaagaaatt caaaaaatga
     7261 cgaaaaatct acttaacgat tttgggaaga ttgatcaaac aaaaccaaaa gcatggactt
     7321 ggggagaata tctatttgtt aaccatggat tgacagggat acgaggggaa gccaaaagag
     7381 gttatccttg tgtttttgac cgaggattgc cttattatca agcatatcaa gggtcgcaac
     7441 aagataagat gatagacact ttactttttc tatcaatgga agtagaagat accaatttga
     7501 tcaaacgaag taataataga catgtttttg aagagtatca attacttatt cgtccttatt
     7561 ttgaattagg gggtgtaaaa accatacaag gtaaaaaata tttggattac ctaaatcagc
     7621 aatttaaaaa gaaaaattgg agtattggtg gaagtgcaga cttattgatc cttacaatct
     7681 ttttagataa aatcatggac aaaggatggt tatgttgata ctataataca ttattttgta
     7741 ttagtaagct aataactaaa aaatggttca atacaaatat tttataaaaa atggtagtgc
     7801 ctatcctttc agattgtaga caaagaaaaa tttctttgtc tacaattttt ttgttaatga
     7861 cttcagtcag tttcgtgcgc aaagcgtgcg aaacaagaaa accatgcgtt tctacacgtg
     7921 gaagaactag catatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagccca
     7981 tactcgttgg aattttaagt atcatattgt atttacacca aaatatcgac gaaaagtaat
     8041 ctataggcaa ttacgaaaag atattggaaa aatacttagg aagttgtgca aaataaaaaa
     8101 cgtggaaatt atagaagcac atgcaacgcc agatcatatt cacatgcttg ttcaagtacc
     8161 atcaaaaata agtatatcca gttttatagt tatctgaaag atcgtagtgc tgtgctaatc
     8221 cacgagcagc acgtaaatca caaatataga tacggaaata aatcattttg gtcggaggaa
     8281 tattatgtga gtacagtcga attgaatcaa aagacaattg aaaaatacat ccgtgaacaa
     8341 gaagctgatg atcgcatttg agatagtata agtaaaagag agtagatgtt gcaataacaa
     8401 aagggtaaaa atggactacc tccagtaaaa taccgaaaaa tagtttaact tttggagata
     8461 agtccattta agggtctata gaatgacaat ttttttaggg gattttacta ttattacttc
     8521 aagacccgct tggattcgca aagtgagtga actcttaaaa ctacctgata gtaggttgtt
     8581 ttaagagtta tctgaaagta aaaattgcat ttatattttt gataaatacg taaatttata
     8641 tatacgtatt tatcaaagtt ttaggccaaa ggaatattgt gtaaatacac taggattgaa
     8701 tgaatagacc agaagaaatt gcgtccaaga acaaaagaag catgatagtg ctctatataa
     8761 attgggcatt caagaatctg aaatttaaag gtagaatttt agttagactt ttagatttta
     8821 aacacaaaaa taaccataca agcaacaaga agcaaagtgc tatattataa attgtccgtc
     8881 cgtttactta cataagatca ttggggctat ttttaaaaga gaataataga taataaatga
     8941 aaaaaaagtc aagtccacaa tcctgtgtaa aatactatac aatgctctca ctatttttta
     9001 cttggcaaat ttaatatatt ttcttgatga agaaagccac tcatcgtgtt gatcaataag
     9061 gactgctcca attaaacgat tggctgaaac gcagtttgga aagatacgaa taattttttc
     9121 tcttctgcg
//
LOCUS       DACEPD010000091         8817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.095, whole genome shotgun sequence.
ACCESSION   DACEPD010000091 DACEPD010000000
VERSION     DACEPD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.095"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(310..807)
                     /locus_tag="IW797_002282"
     CDS             complement(310..807)
                     /locus_tag="IW797_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ1225043.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(847..1656)
                     /locus_tag="IW797_002283"
     CDS             complement(847..1656)
                     /locus_tag="IW797_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1225044.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1990..2847
                     /locus_tag="IW797_002284"
     CDS             1990..2847
                     /locus_tag="IW797_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1225045.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2840..3742
                     /locus_tag="IW797_002285"
     CDS             2840..3742
                     /locus_tag="IW797_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ1225046.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3775..4125
                     /locus_tag="IW797_002286"
     CDS             3775..4125
                     /locus_tag="IW797_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1225047.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4159..5097
                     /locus_tag="IW797_002287"
     CDS             4159..5097
                     /locus_tag="IW797_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ1225048.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5175..6068
                     /locus_tag="IW797_002288"
     CDS             5175..6068
                     /locus_tag="IW797_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1225049.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6106..6669
                     /gene="amaP"
                     /locus_tag="IW797_002289"
     CDS             6106..6669
                     /gene="amaP"
                     /locus_tag="IW797_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1225050.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6683..6871
                     /locus_tag="IW797_002290"
     CDS             6683..6871
                     /locus_tag="IW797_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1225051.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6884..7786
                     /locus_tag="IW797_002291"
     CDS             6884..7786
                     /locus_tag="IW797_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1225052.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7818..8060
                     /locus_tag="IW797_002292"
     CDS             7818..8060
                     /locus_tag="IW797_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1225053.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8316..8687)
                     /locus_tag="IW797_002293"
     CDS             complement(8316..8687)
                     /locus_tag="IW797_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225054.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3073 a         1522 c         1705 g         2517 t
ORIGIN      
        1 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
       61 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
      121 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
      181 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
      241 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
      301 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
      361 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
      421 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
      481 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
      541 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
      601 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
      661 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
      721 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
      781 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
      841 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
      901 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
      961 gctgtaattc aacatatcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     1021 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     1081 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     1141 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     1201 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     1261 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     1321 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     1381 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     1441 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     1501 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     1561 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     1621 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     1681 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     1741 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     1801 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     1861 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     1921 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     1981 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     2041 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     2101 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     2161 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     2221 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     2281 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     2341 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     2401 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     2461 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     2521 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     2581 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     2641 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     2701 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     2761 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     2821 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     2881 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     2941 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     3001 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     3061 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     3121 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     3181 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     3241 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     3301 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     3361 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     3421 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     3481 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     3541 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     3601 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     3661 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     3721 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     3781 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     3841 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     3901 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     3961 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     4021 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     4081 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     4141 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     4201 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     4261 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     4321 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     4381 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     4441 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     4501 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     4561 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     4621 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     4681 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     4741 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     4801 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     4861 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     4921 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     4981 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     5041 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     5101 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     5161 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     5221 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     5281 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     5341 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     5401 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     5461 acgtgaaagt tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     5521 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     5581 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     5641 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     5701 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     5761 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     5821 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     5881 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     5941 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     6001 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     6061 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     6121 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     6181 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
     6241 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
     6301 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
     6361 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
     6421 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
     6481 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
     6541 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
     6601 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
     6661 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
     6721 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
     6781 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
     6841 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
     6901 aaataataac aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
     6961 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
     7021 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
     7081 aaagcatgca cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
     7141 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
     7201 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
     7261 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
     7321 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
     7381 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
     7441 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
     7501 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga
     7561 agtaaaaaat atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa
     7621 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
     7681 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
     7741 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
     7801 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
     7861 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
     7921 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
     7981 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
     8041 tctttgctcg aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca
     8101 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt ggttgggcat
     8161 attatcgaag atgcaagctt ttgaacactg tttatacgga ataagaggct gtgacaagac
     8221 ttttgtcaca gcctcttatt tatgcgggca aattcccaat tagattttta aaagtttttc
     8281 cttaaatgtg tctttattga tacagagaag cttctttaat aaaacaatgc ctccctaaat
     8341 tttcttgaga taggaaaggt gaacatacaa aataatacga ccactttgaa aacccagatg
     8401 atccacaaat ggatcgtatg atactgatga agtaaaaagc aatggatcag aaactcaaag
     8461 agattgaaaa agacaaggaa gaaataatat ctctttttta ctcttgtctg ttttagccaa
     8521 taatagctcg taaagtaatc tagacaaaca aatagaagta aacaaagttt cgtaagagca
     8581 gcaatcgtat catgttgctc tgctagacga ttgacgagca ggaaaaatgt tggtaatgga
     8641 atatagatca cgagtaatga aagaaaaaag taaacaataa atggcatact attcctccta
     8701 attgtgtgag ttcgccctca ttttaaaata acgatatttg tttgtgaagc tttttcgtgt
     8761 cgtaacaaat gttatctaat gtatttttaa ataaataatc attattatca aaataat
//
LOCUS       DACEPD010000092         8668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.096, whole genome shotgun sequence.
ACCESSION   DACEPD010000092 DACEPD010000000
VERSION     DACEPD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.096"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            238..1629
                     /locus_tag="IW797_002294"
     CDS             238..1629
                     /locus_tag="IW797_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1225055.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAEIDFSQEKIYR"
     gene            1688..2491
                     /locus_tag="IW797_002295"
     CDS             1688..2491
                     /locus_tag="IW797_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1225056.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGKRIVPEYNNAIRVFSKRRNPFNHMTVMFD
                     KKAILEVGNYQPLSGFEDYYLWARLLKAGYKGYNIQETLVYARTGKDMYARRGGMKYL
                     GSGLMGRYRIWKDGLGSVKDFIIVSCGHIFISLLPNQLRGKFYQTQLRK"
     gene            2524..3378
                     /locus_tag="IW797_002296"
     CDS             2524..3378
                     /locus_tag="IW797_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HAQ1225057.1"
                     /translation="MKKIQQITLKMSQEIVDFCLKNQLLCYFCGGGAIGTVREQGFIP
                     WDDDLDFFMPREDYEKFYQLWVNSEKSKKYPIQKASKNYNDHNSFTTIRDADTTFIKT
                     YQRDLDIVHGITIDIFPLDVAPVSKFSRKIQKLWALVYALFCSQVVPEKHGGIMATGS
                     KILLSIFRSPRTRYKIWSFAEKQMTKYNNEPTGYVTELCVGPKYMGNVYPAKDFESAV
                     WVPFEDTEMPVPVGYDNYLSQVFGDYMQLPPEKDQVSHHEAVYIDPEHPYTMYKGKYY
                     LTKGAEKK"
     gene            3375..4076
                     /locus_tag="IW797_002297"
     CDS             3375..4076
                     /locus_tag="IW797_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAQ1225058.1"
                     /translation="MITAIIIAGGVGKRMGQDIPKQFITIEGKPIIIYTLESFQHHPQ
                     VDRILVVCKSGWEETMWAYIKEFNISKVKWVITGGSKGQESINNGVQFLKDYSEDDDT
                     IIIHDGIRPLVDELVLSDVIVKCKEFGNGVTSLPYNEQIFIKKTEETTEQYVDRNTLR
                     RVSTPQAYQYGKLRAAYDRAVKEDIGMTDSSYTNTMMVDLGETLYFAAGSDKNIKLTT
                     TDDLELFKAYLKMKD"
     gene            4084..5151
                     /locus_tag="IW797_002298"
     CDS             4084..5151
                     /locus_tag="IW797_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1225059.1"
                     /translation="MMTLIKQPMYQEDLAKVINHTPKIKQLHGKSVLMIGASGMIGSF
                     LIDTLMAANEQLGIKIKVYAMGRNRQKLEKRFASYLELDTFEIVEGDVTEPLPKEIQA
                     DYLIHGASNTHPKAYATDPIGTIMTNLAGTEQVLKHAVATQPVRVLFLSTVEIYGENR
                     GDIEKFTEDYCGYIDCNTLRAGYPEGKRVSESLCQAYIAKHDLDIVIPRLCRTFGPTM
                     LLSDTKASSQFILNAVEQKDIVLKSEGNQYFSYVYVGDAVSAILHLLLEGEKGEAYNV
                     SSENFDLRLKELAQQLADISGKQVIFELPDEIEQKGFSKASTAVLENKKIKETGWKPI
                     FPLNESLVHTVELVKGEKYNV"
     gene            5144..6109
                     /locus_tag="IW797_002299"
     CDS             5144..6109
                     /locus_tag="IW797_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1225060.1"
                     /translation="MCEISIIVPVYKVEKYLRKCVDSILAQTFTDFEVILVDDGSPDN
                     SGKICDEYAEKDNRVRVIHKENGGLSSARNAGIDVARGKYLGFVDSDDYIDEDMYEIL
                     YENLKIHDADISSVELIPFYGDRYKKANKEKKVIILNKKEAIKSVLEGTQFYAYAWNK
                     LYRKELFKDNRYLDGKTFEDAYIIIDLLFQTEKIVVSNEEKYFYLQRNDSIMGKSFSM
                     NNFDVIEAWQYNKEKILDAFPDLHDSYYRRLCWAYFYVLDKMVLSSSYQQIPRKKTVI
                     EFLKKNRNFILTYKGFTKSRKIAIIALSFRVDWYRKLVEQKNKKN"
     gene            6154..7128
                     /locus_tag="IW797_002300"
     CDS             6154..7128
                     /locus_tag="IW797_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ1225061.1"
                     /translation="MCRISIIVPVYNVEKYLKKCVDSILGQTFTDFELLLIDDGSTDN
                     SGSICDELAKTDNRIKVIHKENGGLSDARNIGIEVAKGDFIGFIDSDDYIDEDMYAFL
                     YNNILKYDAELSMCGIYDVHKNKEIKKLTPFSQLVTKSEAIELVLDGKLVVANAVSKL
                     YKKELFENVRYPKGKIAEDAAVILKIINQCNKIHVDTSQKYYYYHRGDSITSKKFTKK
                     DFDIIDVWNENEKWIIKNYPQMYDKVHTRVCWAYFVVLDKLVLTEEKGLYQEKKQVRE
                     YLIDNFFFIIKNKRLTFQRKISMLLLTFGIAAYKIPVKYQYKKVKAVN"
     gene            7199..>8668
                     /locus_tag="IW797_002301"
     CDS             7199..>8668
                     /locus_tag="IW797_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAQ1225062.1"
                     /translation="MFIYILRIVIQFVGRSFFIYFLGAKYLGLNGLFTNILSFLSLAE
                     LGIGSSIIYSLYKPLAQKDESKVKALMNLYKKTYEFIGIFIGIVGVLIIPLLPLFIKN
                     STDIPHMYRYYVLFLLNSVLSYFFTYKRSLAIADQKNYLVAINDFAFLFFMNIVQVIA
                     LYFYSNFTLFLIVQILFTLFSNINISRTVDKRYSYLKEKDVVSLDKQTKDEVKKNVIG
                     NMSSKIGGVIVMGTDNILISTFVNLTAVGIYSNYSLIVTSVQNLCKQITNSITASVGN
                     FAVSNKKKDSFELFKKHFFINHSLIFFSSILLLTLMNPFISLWVGAKYMLPMSTTILI
                     VLNYSVQVYRNTSFVFIESFGLYWSQRKKPIIEAGVNLLLSLLLLIVFDMGINGVLIG
                     TIGSSLGFVIWYEAFIIYKTVFEKRFIHFFLTFWRSFIELIISAIVVFLLNEYLANGG
                     STFLQFITKTLITIVTTSILYLLLYFRREEFTFIKKILFR"
BASE COUNT         3102 a         1159 c         1595 g         2812 t
ORIGIN      
        1 tatatgaata ttgtaacttg tgaataagga attagcagga gaatactagt tcagcataaa
       61 ggaagtgatg ggacttctga gacaactttc tgttatgcag aaatacacat ataaacagac
      121 aataaacgct tttccccgtt tcttttaatt tcctttacaa tttggttatt cactaggttt
      181 ttaaactttg ctatggtaag atgatagagg cattatttta tggttaggag tgtatatatg
      241 aataaaaatg gggagtggaa tgcggcacgc agaatattca ttattcttat ggatgtactt
      301 gtattaaatg tatcgatcta tcttagtttt ttgctgaaat tccagggaga agtaccagtg
      361 agaaacttag aaacctttca gcattcagca atttttattt ccatcatctt tattgcgttg
      421 aatattttgt taggagctta tgtattctat aaccgaatga tcagtgatat tgtcttcatt
      481 actgttattg gacaagtact aatagcattg gggatcatgg tagttacttt tgctggccga
      541 tggtttgcat ttccgcgtgg cgtcattttg atctcgttta tcctaggtac gatattactt
      601 agtgtttacc ggatagtcgt ttacaaactg tacttaagag tcagtcgtga taagaaagtc
      661 cttatcgtgg gtttggagaa agatgtcgca ccagctatcc aaaattttca atctaagaaa
      721 aacaataaac ataaggttga agcagtggca atcgataatt attacgaaaa tacaaaaaaa
      781 ttgatcgatg atatggatat cgtctatttg gcttctaaaa tcccagaaga tgaaaaaatc
      841 aacatttatc aattagtgac taaaaaagag aagaaattat ttttaaatac gacttttgag
      901 aatctgacaa tgataaatcc gaatattatg aattttgaag atgaaagcat cattgaagct
      961 tctgggttca gaatccaacc agaatacgaa ctattcaagc gtctctttga tatcatcgtt
     1021 tcgcttattt tactgatcct cgcctcacct tttatgctaa tcacagcgat tttggtaaaa
     1081 acaacttcac ctggaccaat catctataaa caagtacgta ttacgaaaaa tcaaaaggaa
     1141 ttttcaatct ataaattccg ttccatgagt gcgacagctg aagcaaaatc aggaccagtc
     1201 ttggctaaaa gtaacgatgc acgtgtgaca ccagtaggta aatttatccg tgctgtacgt
     1261 tttgacgaat tacctcaaat ctttaatgta ttgaaaggcg atatgtcgat tgtcggtcca
     1321 aggccagaaa gaccattttt cgttgatcag ttcaatgaag aaaatcctta ttattactta
     1381 agacacaatg tacgtgcggg aattaccgga tatgcacaag tatacggaaa atatgtatca
     1441 gattataaca gtaaactacg ttttgatttg ttgtatatca aaaaatattc attactatta
     1501 gatttgaaga ttttacttca aacaatcaaa atcttatttg ataagatgtc atctcaagga
     1561 ctagatgaag atgaaaatat tagttcatta gcagaaatcg acttttcaca agagaaaatt
     1621 tatcgctaga ttggaaaaat tttgagcaaa gaggtaggag agaacattgg aaaataaaaa
     1681 tgtaagtatg ctgctttctt tatatgataa agaaaagcca gagtatttaa aggaagcatt
     1741 aactagtatt tttgagcaaa cagtaatgcc aaaagaaatt gtattagttt atgacggccc
     1801 tataaatgaa aaacttcaag tagttgtcac ttatttccaa caaaaagatc ctcacttgtt
     1861 tacagttgta aaattagaaa aaaatcaagg attggggatt gctttagcaa ttggattaga
     1921 acatacgaaa aatcgcttag tagccagaat ggatacagat gatataatgg aaccccaacg
     1981 attagaaaaa caattagaga tcttccaaaa agatccagaa atagcgattg ttgggagtaa
     2041 tattgaggaa tttactggca cgcttagcaa tgttatcgga aaaagaattg tacctgaata
     2101 taacaatgca attcgtgtat tttctaaaag aagaaatccc tttaatcata tgaccgttat
     2161 gtttgataaa aaagctattt tggaagtggg aaattaccaa ccattaagtg ggtttgaaga
     2221 ttattatctt tgggcaagat tattaaaggc aggttataaa ggatataata ttcaagaaac
     2281 attagtctat gcgcgaactg gcaaagatat gtatgcaaga agaggcggaa tgaaatatct
     2341 tggttctggt cttatgggtc gttatcgtat ctggaaagac ggtttagggt cagtaaaaga
     2401 ttttataatt gtttcttgcg gacatatttt tattagctta ctaccaaatc aattaagagg
     2461 aaaattttat caaacgcaat taagaaaata ggaggttttt ctttgtatag cactgatcca
     2521 aaaatgaaaa aaatacaaca gattacgttg aaaatgtctc aagagattgt tgatttttgt
     2581 ctaaaaaatc aacttctttg ttatttctgc ggtggtggtg ccatcggtac tgtgagagaa
     2641 caagggttta ttccatggga tgatgattta gatttcttta tgcctagaga ggattatgaa
     2701 aaattttatc aactatgggt taattcagaa aaaagtaaaa aatatcccat acagaaggct
     2761 tctaaaaatt ataatgatca taattctttt acaactatcc gcgatgcaga tactacattt
     2821 attaaaactt atcaaagaga tttagatata gtccatggaa ttactattga tattttcccg
     2881 ttagatgttg cccctgtttc gaaatttagc cgtaaaattc aaaaactatg ggctttagta
     2941 tatgctttat tttgttctca agtcgtccct gaaaaacatg gtggaataat ggctacaggt
     3001 agtaagatac tcttatctat tttccgctca cctcgtacga gatataagat atggtctttt
     3061 gctgaaaaac aaatgacaaa atataataat gaaccaacgg gctatgtgac tgaactttgt
     3121 gtcggaccta aatacatggg aaatgtttat cctgcaaaag actttgaatc tgctgtgtgg
     3181 gtgccattcg aagatacaga gatgccagta ccagttggtt atgataatta tttaagtcaa
     3241 gtttttggtg actatatgca gcttccgcct gaaaaagatc aggtttctca tcatgaagca
     3301 gtttatattg atccagaaca tccatacaca atgtacaaag gaaaatatta tttaactaaa
     3361 ggagcagaaa aaaaatgatt acagctatta ttattgctgg tggtgtgggt aaacgaatgg
     3421 gacaagatat cccaaaacag ttcattacaa ttgaaggaaa acctattatt atctatactt
     3481 tagaatcatt tcaacaccat cctcaagttg accggatttt agtcgtttgc aaatctggtt
     3541 gggaagaaac gatgtgggcg tatatcaaag aattcaatat tagcaaagtc aaatgggtca
     3601 ttacaggtgg atcaaaggga caagagtcga tcaataatgg tgttcagttt ttgaaagact
     3661 attctgaaga tgatgatacg atcatcatcc atgatggaat ccgtccacta gtagacgaac
     3721 tggtattgtc agatgtaatc gttaaatgta aagaatttgg taacggtgta acctctctac
     3781 cttacaacga acaaattttt atcaagaaaa cagaagaaac gacggaacag tatgtcgatc
     3841 gtaacacatt gaggcgtgtc tcaactccac aagcttatca atacggaaaa ctacgggcag
     3901 catatgaccg ggcagtgaaa gaagacatcg gtatgactga ttcttcttat acgaatacca
     3961 tgatggtaga tttgggagaa actttatatt ttgctgctgg ttctgataaa aatatcaaat
     4021 tgacaacaac agatgatcta gaattgttca aagcatattt aaaaatgaaa gactaggtga
     4081 aagatgatga cgttgattaa acaaccaatg taccaagaag atttggcaaa agtaatcaat
     4141 catacaccaa aaataaaaca gctacatgga aaatccgttc tgatgattgg tgcttctgga
     4201 atgatcggct cttttttgat tgatacgttg atggctgcta atgaacaact aggcatcaaa
     4261 atcaaagttt atgcaatggg cagaaaccgt cagaagcttg aaaaaaggtt tgccagttat
     4321 cttgaattag atactttcga aattgttgaa ggtgacgtca cagaaccctt accaaaagaa
     4381 attcaagctg actatttgat acacggtgca agtaacacac atcctaaggc ttacgcaact
     4441 gatccaatcg gtacgatcat gactaatctt gcaggtacag agcaagtatt gaaacatgca
     4501 gtagcaaccc aaccggttag agtattattt ttatcaacag tagaaatcta tggcgaaaat
     4561 cgaggcgata tagaaaaatt cacagaagat tactgtggat acatcgattg taatacactg
     4621 agagcaggct accctgaagg aaaaagagtc agtgaatcat tatgccaagc ctacatcgca
     4681 aaacacgacc tcgacatcgt gattccaagg ctttgccgta cgtttggtcc gacgatgctt
     4741 ttatctgata cgaaagcatc ctcacaattt attttaaatg cagtcgaaca aaaagatatt
     4801 gttttaaaaa gtgaaggaaa ccaatatttt tcgtatgtat atgttggaga tgctgtttct
     4861 gctattcttc atttactgct tgaaggagaa aaaggagagg cgtacaacgt ttcttcagaa
     4921 aactttgatt tacgactaaa agaattagcc caacagctag ctgatatcag cggcaaacaa
     4981 gtaatttttg aattgccgga tgagattgaa caaaaaggtt tctcaaaagc tagcacagca
     5041 gtacttgaaa ataaaaaaat taaagagaca ggttggaaac caatttttcc tttgaacgaa
     5101 tcattggttc atacagtaga attagttaaa ggtgagaaat ataatgtgtg agattagtat
     5161 tattgttcct gtgtataaag ttgaaaaata tttaagaaaa tgtgtagatt cgattttagc
     5221 ccaaacattt actgattttg aagttatatt agtagatgat ggatctcctg acaatagcgg
     5281 gaaaatctgt gatgagtatg ctgaaaaaga taatcgagta cgtgtgatcc ataaagaaaa
     5341 cggcggatta agcagtgcta gaaatgcggg gattgatgtt gcgagaggca aatacttagg
     5401 ctttgttgac agtgatgatt atattgatga agatatgtat gaaattttgt atgagaattt
     5461 gaaaattcat gatgccgaca ttagctctgt tgaattgatt cctttttatg gtgatagata
     5521 taagaaagct aataaagaaa agaaagtaat tatattaaat aaaaaggaag caataaaatc
     5581 tgttttagaa ggaacgcaat tttatgcata cgcatggaat aaactttatc gtaaggaact
     5641 ttttaaagat aatcgttact tggatggaaa aacatttgag gatgcatata taataataga
     5701 tctattattt caaacagaaa aaattgttgt ttccaatgaa gagaaatatt tctatcttca
     5761 aagaaatgat agtattatgg gaaaaagttt ttctatgaat aatttcgatg ttatagaagc
     5821 atggcaatat aacaaagaga agattttaga tgcttttcca gatttgcatg attcctatta
     5881 tcgccgacta tgttgggcat atttttacgt gttggataaa atggttttat caagtagtta
     5941 tcaacaaatt ccacgtaaaa aaacagtaat tgaattttta aagaagaaca gaaattttat
     6001 attaacttat aagggattca ctaaatcgcg caaaatagct attattgctc tgagctttag
     6061 agtagattgg tataggaaat tggttgaaca aaaaaataaa aaaaattaga atgtttaaat
     6121 tagtaaataa aagaaaagga attgaaaact aaaatgtgca gaataagtat aatagttcca
     6181 gtttataatg ttgaaaaata tttaaaaaag tgtgtcgatt caattctagg ccaaacattt
     6241 accgattttg aattattatt aattgatgat ggttctacag ataattcagg aagtatatgc
     6301 gatgaacttg cgaaaacaga caaccgaata aaagtgatac ataaggaaaa tgggggtcta
     6361 agtgatgcca gaaatattgg tattgaggta gcaaagggag attttattgg gtttatagat
     6421 agtgatgatt acattgatga agatatgtat gcatttttat ataacaatat attgaaatat
     6481 gatgcagagt tatcaatgtg tggtatttat gacgtccata agaataaaga aattaaaaag
     6541 ttaactcctt tttctcaatt ggttactaaa tctgaagcaa ttgaattagt attagatggt
     6601 aagctagttg ttgctaatgc agtaagcaag ctatataaaa aagaattatt tgagaatgtg
     6661 agatatccaa aaggaaaaat tgctgaagac gcagcagtaa tactgaaaat tatcaatcag
     6721 tgtaataaaa tccatgttga tacttctcaa aaatattatt attatcatcg tggagatagt
     6781 attactagta aaaaatttac aaaaaaagat tttgatataa ttgatgtatg gaatgagaat
     6841 gaaaaatgga ttattaaaaa ttatcctcaa atgtacgata aagtacatac tagagtttgt
     6901 tgggcttact ttgttgtgtt agataaatta gttttaactg aagaaaaagg actgtatcaa
     6961 gaaaaaaaac aagtaagaga gtatttaatt gataatttct tttttattat aaaaaataaa
     7021 cgattaactt ttcaaagaaa aatatctatg ttattgctta cttttggaat tgctgcgtat
     7081 aaaataccgg ttaaatatca gtataagaaa gtaaaggctg ttaattaatg ctttagttag
     7141 gatgaattag atgaaaaaaa gcaggttgga gtattcttta ataaattctt ctatatcaat
     7201 gtttatatat attttaagga tagtaataca atttgtaggt agaagttttt ttatttattt
     7261 cttaggagcg aaatatttag gattaaatgg tttatttaca aatatactta gtttcttgtc
     7321 gttggctgaa ctaggcatag gctcttctat tatttattcc ctatacaaac cattagccca
     7381 aaaggatgaa agtaaagtaa aggctttaat gaatttgtat aagaagacat atgaatttat
     7441 tggtattttt atcggcatag taggagtatt gattattccg ctgttacctc tatttattaa
     7501 aaatagcaca gacataccac atatgtatcg atattatgta ttatttttat taaattcagt
     7561 tcttagttat tttttcacgt ataaaagatc acttgcaata gcagatcaaa aaaattatct
     7621 ggttgcgatt aatgattttg cctttttatt ctttatgaac attgtacaag taattgcttt
     7681 gtacttttat tctaatttta ctctatttct aatagtgcag attttgttta ctctattttc
     7741 taatataaat atttccagaa cagttgacaa gagatattca tatttaaaag agaaagatgt
     7801 agtttctcta gataagcaaa caaaagatga agtaaagaaa aatgtcatag gtaatatgtc
     7861 ctcaaaaata ggcggggtta ttgtaatggg gacagataat atattaattt ctacatttgt
     7921 taatttaaca gctgtaggaa tttattctaa ctattcattg attgtcacaa gtgtgcaaaa
     7981 tttatgtaag caaattacta attctatcac ggcaagtgta ggaaattttg ctgtatcaaa
     8041 taaaaaaaag gattcatttg agctatttaa aaaacatttt tttatcaacc actcactaat
     8101 ctttttttct tctatattgt tattgacttt aatgaatccc ttcattagct tgtgggtagg
     8161 agccaaatac atgctaccta tgtcaacgac tattttaatt gttttaaatt atagtgtaca
     8221 ggtttataga aatacatcct tcgtttttat agagtcattt ggtttatatt ggtctcaacg
     8281 gaaaaagcct ataattgaag caggtgtaaa cttgttatta tctttgcttt tgttaatagt
     8341 ttttgatatg ggaatcaatg gagtacttat agggactatc ggatcgtctc taggatttgt
     8401 gatttggtat gaagcattta tcatatataa aacggtattt gagaaacgtt ttattcattt
     8461 ctttttaact ttctggcgaa gttttataga attaataatt tcggcaatag tcgtttttct
     8521 attaaatgaa tatttagcaa atggaggcag tacgttcctt caatttataa ctaaaacctt
     8581 aataactata gtaacaacat cgatattata tttgttatta tattttagaa gagaagaatt
     8641 tacatttatc aaaaaaattc tttttagg
//
LOCUS       DACEPD010000093         8616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.097, whole genome shotgun sequence.
ACCESSION   DACEPD010000093 DACEPD010000000
VERSION     DACEPD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.097"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(193..1146)
                     /gene="menA"
                     /locus_tag="IW797_002302"
     CDS             complement(193..1146)
                     /gene="menA"
                     /locus_tag="IW797_002302"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ1225063.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1159..2262)
                     /locus_tag="IW797_002303"
     CDS             complement(1159..2262)
                     /locus_tag="IW797_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1225064.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2352..3287)
                     /locus_tag="IW797_002304"
     CDS             complement(2352..3287)
                     /locus_tag="IW797_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ1225065.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3589..5523
                     /locus_tag="IW797_002305"
     CDS             3589..5523
                     /locus_tag="IW797_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ1225066.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5980..6396
                     /locus_tag="IW797_002306"
     CDS             5980..6396
                     /locus_tag="IW797_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ1225067.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6410..6976
                     /locus_tag="IW797_002307"
     CDS             6410..6976
                     /locus_tag="IW797_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ1225068.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6999..7985
                     /locus_tag="IW797_002308"
     CDS             6999..7985
                     /locus_tag="IW797_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ1225069.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8140..>8616)
                     /gene="scm"
                     /locus_tag="IW797_002309"
     CDS             complement(8140..>8616)
                     /gene="scm"
                     /locus_tag="IW797_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ1225070.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2537 a         1667 c         1741 g         2671 t
ORIGIN      
        1 aaagccattt tcacaaaatt tttcttaata ctcgaaacta agcgactttt tcacaacctc
       61 ctgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata
      121 taaggagcta tttttatttg gctgttctta ttacttgatg cagggcagtt ttttcacaac
      181 ctcttcatgc tgttaaatgg cttgcataat gattgttagt aatagcccca atacataact
      241 ggcattgaat aagaccatat ttttgatgga atagctgaaa ctgataggct ggggtaacgt
      301 ttgcttgaat tgatccaaat gcttcttcac aattggcaag gtcaagaagg tgaccaagat
      361 cggccattca tagataccgc tcaacaaccc cattaaaatc acgaaatagc aagcatacat
      421 caatagctgg aataagactg ttcccgcagg acgtccgata taaaaaacta gtgtatagcg
      481 atgattttca atatcacggt caagatcccg caaattatta gccaacatga tattggcaat
      541 cgtaaacacc aagggtaagg aagctaatac gattgctgct atagcccaaa gatttcctac
      601 caaatcaaat tgtccagatg aaaagttgac tgtcagatca tagacatctg aaccgaatgt
      661 attgacataa acgaccatgg caaaaattcc aaggcccata gtcaccccac tgaaaatttc
      721 ccctaaaggc attcgagata aaggaatcgg tccgaatgta tagaatactc caataaaaca
      781 acagataaat cccatcaaaa gtaaaagcca acccgtattg atcgttagaa taacgccgat
      841 aacaaaaaca aatgcaatca tagctaaaat catgtttcga atcaatacag gagatagatt
      901 tctgcggcca atgacatttt cttcatactt atatacttca gaatgagcct tttcataatc
      961 catgtaatta ttgatagcgg tagtcgctaa atcaaaaact agcattccaa taaaaaataa
     1021 aaaagtgttt agccaatgta tctcatgaaa aaaagcgata gagaataata cccctaccgc
     1081 aaaaggaaat acactagcca cctttgttcg tatttcaact acttctaaaa agatttttag
     1141 tgtcatggtt cgtactcctt attttagttc agaacggtca cccatcgtgt aatgggagtc
     1201 ctccgataat ttgaaggtgt ctttgattgg atcggtcaca tatactttat cgtcttttgt
     1261 cacgaagatc gcttccgtcc cgttattcaa ttcgctttcg acgtattcca gtcctttttt
     1321 cacacccatt gaaaagactg ctgtagacaa cccgtcacca tcaattgatt tatctgttat
     1381 gatcgtgaca cttgcgatat cattgtcgaa cggataaccc gtttttggat caaacaaatg
     1441 atggtatttt ttgccatcta cttctaaata acgctcgtaa attccagagg taactaatgt
     1501 tttgttccgt tctttaatag aacctaaaac agatccgcga gccatgttcg gatcttggat
     1561 gccgactgtc cagtcttggt tttcgcctct tgggctgtgg cctaagacat agacattacc
     1621 gcccaagtca acgatggcag tggtcactcc ttgttttttc aagactttca ctacttcgtc
     1681 cgtaatgtaa cctttggcga ttgcacctaa atcgatgatc attccttttt cttttagata
     1741 aacagttttt tctttgtcat ttagttcgat cttatgataa tccactaatt tcaatgcctg
     1801 atcgatttct tcttgggaag gttttctggc gtcatcaaaa ccgattcgcc ataattgtgt
     1861 gattgcacca atcgccatat cgaatccgcc ttgagaatct tggctatatt cgtaggcccg
     1921 cttgactaac gtgtagacgt catcagaaac ttttactggt ttgatcccag cttgctcatt
     1981 cacttcatcg atctcagaac ctttttgatt gatcgtgatt ttatcaccaa gttcttttac
     2041 tcgatcgaag gcaggtttca atgctgattc tttgccttca tcatacacac ggatacgaac
     2101 gtaagtccct aacaagaact gttcatcaga atatggatct ttcaatatct tggtagctgc
     2161 tgaactttca gtggttgttt tatttgctga gttgctgcag ccagttagca ccataaaaga
     2221 gatgcccagt gtgattaagg aaaatagtag ttttttccgc atctttttcc tctcttcatt
     2281 ttgaaaggtg agacgactgc ctaatctggc tatcgtccca ccctccaact tttattagta
     2341 tgattatgtg attatttttc tgttacgata ttatctactt cgattgtttg agtgtcgcct
     2401 ttttcagctg cattcactaa ttgttgtgcg tacatgatga atgaatgtga gctgtgtgta
     2461 gcacctgtaa caacttctac attagcaggt gagccatctt cttcgcccat tgctttgacg
     2521 aattcgtcgt tcagagtagg gatgtaagtt tttggttctg taccgctttt tgctttcata
     2581 ttttcattat attctgtatc atcttgtttt gatttaccgt ctttgtcgat ataatcaaag
     2641 tttgattcag ttactttgcc gccagcaact gtcatttcga attgcacacg gtagccgtta
     2701 gagtagttct tttcttcaag cttgtaagtg ccgtctttca aatcagcacc attgtcgatt
     2761 tcgattgtat ctgtgttacc agcttgagca gcttggatca attgttgtgc gtagttttgg
     2821 aagctttcag atgagtgagt agctccagtt acgacttcca caccgcttgc gctttgtgct
     2881 tttaagaatt ggtcattcaa ctcaggaatg tatttttctg ggtttgtgcc agattttgct
     2941 tccatgtttt tgttgtattc tgcatctttt gtttttgatt cgccgttttc attgacgtta
     3001 tcatattttg attcagtgat tttgccgtct tttacaacca tttcaaagac tgcacggtaa
     3061 ccatttgaat aatttttttc ttctaattta tatgtgccgt cttgcaaatc tccgccagct
     3121 acaacttttg cagatgattc tgttgtgctt tttgcagtcg aagattgtgc tgtagatgat
     3181 tcagaagaag agtttgagct tccgttgtca tctgcgccgc aagcgcctaa cactagagct
     3241 gaaaatgcta atacggcaaa tccagttaca aaacgttttt tcttcatttt aataccccac
     3301 cattccattt ctagtccaca atttttcttt gtgattcatt atacattcta atctttttag
     3361 cctataagtc aactattttt gataaataac ccatgtaata tttcaaacgt tttctttcag
     3421 caaatttcgt gaaaaaacgc tttattttaa aatttgaaaa aaatccaaaa ggagttagac
     3481 aaaagaacag aacatctata taatatatgg agattgtgta ataagtatca aactaaaaga
     3541 attatcatat attcattata tagatagtct ataaggagcg gatgatcaat ggcaaagcaa
     3601 aatattgtcg ttgtaggagc aggatatgcc ggtgtttcag ctacaaaata cttggcaaag
     3661 aaactgaaaa aagaagatgt agcaattact ctgatcgatc gtcattcgta ccatactatg
     3721 atgaccgagt tgcatgaagt agctggcgga cgtgtagaac cagaagcaat ccaatacgat
     3781 ctgcagcatt tgtttgctcg tcaaaagaat gtacaacttg tcacagacac tgtaatcggt
     3841 atcgataaag aacgaaaaat cgtcaagacc aaattaggtt cctatccttt tgatcagctg
     3901 atccttggaa tgggcggaga gccgaatgac tttggtacac ctggtgtcaa agcacacgga
     3961 ttcacccttt ggtcatttga agattctgta aggattcggg aacatattga gaagacagta
     4021 gcaaaagctg cattggaacc agatgatgaa ctgcgtaaag ccatgctgac tttcgttgtc
     4081 tgtggttctg gatttaccgg tattgaaatg attggggaac tgatcgactg gaaaaatcgc
     4141 ctagccaaag attataaatt agatccaaac gaaatcacac tgatggtcgt agaggcaatg
     4201 ccgacgatct tgaatatgtt ggatcgaaat gatgctgcaa aagctgaacg ctatttgcgt
     4261 aagcacaacg tggaattatt attgaatgca ccaatcgtag aagtggctgc tgatcatatc
     4321 aaattgaaag acggacgcaa cattccgact cacacactga tttggacagc tggtgtcaaa
     4381 ggaacttctg acgctgctga ctttggatta gaatcagcac gcggacagcg gttgatcgcc
     4441 aatgaatata tgcaggcaaa aggctacgaa gataaaaaca tctatatcat tggtgattta
     4501 gtcttttatg aagaattccc aggaacaccg acaccgcaga tcgttcaagc tgctgaacaa
     4561 acaggacaca cagccgcagc gaatatcgtg gcttcgatca aaggcggaga aaagcataaa
     4621 tttaaaggaa actatcaagg atttatggtt tctattggtg ccaaatgggg ggtagctaac
     4681 ctatttgata agatccattt aagcggattt ttagctatcg taatgaaaca tatcgtgaat
     4741 ttgaaatact tctttgatat tcgttctggt tattatatgt tccaatattt aatgcatgag
     4801 tttttccata tcaaagatga tcgcaacgtg actcgaggac attcttctcg ttacggcaat
     4861 gtcttatgga gtgtgccact tcgtattttc tatggtttag tttggttcgt tgaagcttcc
     4921 aaaaaaatca ttgggaatgg tgaagtgttc aaaccaagta cttggtttgg tgaaggatca
     4981 tggttcactg gaaacagtgt gttcccttgg acatgggaat acaatgtaac agatgttgcc
     5041 agtggtgctt ctgctgctgg tgatgcaacc acagctgcca gcggagcagg ggcaagtgcc
     5101 ggagaagcag cgactcaagc tgcacatttt ggtttgagtt atgcatacgg tgaagaacca
     5161 atggctgtga ttgagaagtc accagactgg ttcacaaaga tcatgcaagt gatcatgcca
     5221 aacaatgatg tagcaatgtt cttccaaaaa ttcatggtat tctttgaaat tgccttggca
     5281 ttagcattga ttgctggatt attcacatgg ctatgcagtg cgacaacgat tgtattggtg
     5341 atcacgttct gcttatcagg catgttttac tgggtaaaca tttggttcat ttttgttgct
     5401 ttcgcattga tgaacggttc cggacgtgcg attgggttgg atcgctgggt aattccatgg
     5461 ctacaacgta cactaggtaa ttggtggtac ggtagaccaa gttcccagta cggcaagaaa
     5521 taaatagata aagaacgtta tttgaatgca agatttcaat agaaaggggc tgagacaaac
     5581 cgtcacagcc tcctttttat gtaaaattaa aggaataaga tgcccgagat aagcctccat
     5641 gagcaagaca caggtgtcac aacctctgtt acaacctctt gttataaacg gtgttccaga
     5701 agtagcttct tcgaaaaata agccgaaatt tcacaaaaac ttgaaaagta attttcgtaa
     5761 attccttctt atttcttgaa gttgcccact tctgtcacaa cctcttgtta taaacggtgt
     5821 tccacctgct gcttctcggt aataagccca aagaattaga aaacatggaa agctgttttc
     5881 tattcttcgt tcttattact caaagcaaga cacaggtgtc acaacctctt gtttttatgc
     5941 taaaattggg tgagttaatc tggaaaaagg agaacaaaaa tgagccttaa agaatttgct
     6001 gaaaaaagtc tgctcaaacc ttgggatgtt tttattattt tgcttttgac tgttctttct
     6061 tttttaccag tcatgatttt tggtcttcag caaaacaccg caacagcaga aaaagaagcg
     6121 gtactgcgtg tggatggcac agaaatcaaa acattccctt tgatggctgg agaagagagc
     6181 aacacatata cttacaaaga tgaacacgga gattataact tgatcgagat agatggcgat
     6241 cgagtacgga taaaagaagc agattgcgat gaccagatct gcgtacgtcg aggctgggca
     6301 acgaaaaatg gcgaaaccat cgtctgtttg ccacataaat tagtgatcga agtccgttca
     6361 acagatggag gtgacgaaga cagtctgatt tattagacct tcacacacca tgagtaaatt
     6421 acaaaaaatg atttatattt cgctacttgt tgcacaagga gtgatcattg gtttattgga
     6481 aaatatgatc ccttatcctt tttcttttgc gcctggtgca aaactaggct tagccaatct
     6541 aatcacgatc gttgccattt ttaccatgaa aaaacaagac agcttcttac tactttggct
     6601 acgactattc ctgactactt tgctaggcgg aacgatttcc acattccttt atagtatgag
     6661 tggtgctttg cttagttatt ttggaatgtt attggtcaag caattaggac caaaacgtgt
     6721 cagtatcatc ggaattagtg cggccggcgg tttcatgcat aatgtcggac agcttttgat
     6781 ggcttctttc attgcacaat catggacagt catgttgtac ttgccagtcc tttcagtttt
     6841 agggatttta tcagggattg caatcgggat cgcagcgaat tatcttttac gacatgtgca
     6901 gacacttcaa cgattccaaa aagattatga gcagggaacc aaacaaaaat ggagcccaat
     6961 gtttaccaaa aaataatcgt cacacaagga gtttttttat gaagctacat ccaatgtggg
     7021 aaaactatcc tgctttaagt aaagaattga cagaaacact aaaacggatg gaagaaactg
     7081 tccgcctgaa aaacaaacct gtcgaacaag ccatcaaaga aacgatccat gccggcggaa
     7141 agctgttgcg tcctgcctat cagctattgt tctcacaatt tggaccagag caagatcgag
     7201 acaaagccat cgctttggca gcttctatcg aaatgctcca tacggccaca ctgatccatg
     7261 atgatatcgt tgatgaagcg gatctgcgac gtggtcagcc taatatccgt agtcagtttg
     7321 gtaatgtcgt ttctgtctat gcaggagact atttatttgt ctgctgtttt aaactattgt
     7381 ctgattactc cacttccttg aaaagtctcc aattgaattc tcgcagtatg gaaaaagtat
     7441 tgaatggcga attagggcag atggatgatc gctataatta tgaactttct gtaaaagaat
     7501 atttggaaaa tatttcaggt aagacagctg aactgttcca attaagttgt tctgttggcg
     7561 cttttgaaag tggaacaagc gagcgcttcg caaaaaaagc aggagatatc ggactttcta
     7621 tcgggatggc ctttcagatt atcgatgata ttttagatta tacaaaagag agccaggaaa
     7681 tcggcaaacc tgtactagaa gacatgcgtc aaggggtcta ttctttaccg ttgatctact
     7741 ctttgcaaaa aaacaaacca aagctactac cttatttaga gaagaaagcg gcaatgacag
     7801 aagaagatgt cgacgccgta cgcaaaatcg ttgaacatac aggaggcgtg gaagaagcta
     7861 ggaagttagc ggcctcttac acagaaaagg ctttgaagga aataaagaaa ttacctgcca
     7921 cctctcttcg aacaaaagag aatctctttt cccttaccca actgattttg gatcgcaaag
     7981 actgaatagg gaaaggttcg atacgtaaat aaatttctca gaaggaaatc tacgtgtttt
     8041 ttgacaaatt gaattgtagt tcttgtattt atgataaaaa gcaaaaataa agagcaacgt
     8101 ccgttagcga cgttgctctt ttttatcttg aatatttttt taccttttcg atttcttata
     8161 actcataaaa gtgattctta aaccgattac aagtaatata acgcctgcga gtgttatcca
     8221 attattcgat tgacttccag ttttaggaag tgctcttttt gatttgtttc cattgtcatt
     8281 gcttgtttta ttagttaagt taaagttcgt cctgttatcc gaacttgttt ttgagaagga
     8341 gacacttgca tgacgtgtat cgcttgtggt agacgacgtg tctgtactat cgcttgaagt
     8401 tgatgaatct gagctatcac ttgtggtaga ggatgtatct gtactgtcac ttgaagctga
     8461 cgtgtccgtg ctatcacttg tagtggtaga tgaatctgag ctatcacttg tggtagaggt
     8521 agtatctgta ctgtcacttg aagctgacgt gtccgtgcta tcacttgttg tggtagatgt
     8581 atccgtactg tcgcttgaag ctgacgtgtc cgtgct
//
LOCUS       DACEPD010000094         8512 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.098, whole genome shotgun sequence.
ACCESSION   DACEPD010000094 DACEPD010000000
VERSION     DACEPD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.098"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(30..587)
                     /gene="frr"
                     /locus_tag="IW797_002310"
     CDS             complement(30..587)
                     /gene="frr"
                     /locus_tag="IW797_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ1225071.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(590..1312)
                     /locus_tag="IW797_002311"
     CDS             complement(590..1312)
                     /locus_tag="IW797_002311"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ1225072.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1448..2329)
                     /locus_tag="IW797_002312"
     CDS             complement(1448..2329)
                     /locus_tag="IW797_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ1225073.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2428..3210)
                     /gene="rpsB"
                     /locus_tag="IW797_002313"
     CDS             complement(2428..3210)
                     /gene="rpsB"
                     /locus_tag="IW797_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ1225074.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3569..4048
                     /locus_tag="IW797_002314"
     CDS             3569..4048
                     /locus_tag="IW797_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ1225075.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4265..5356
                     /gene="phnW"
                     /locus_tag="IW797_002315"
     CDS             4265..5356
                     /gene="phnW"
                     /locus_tag="IW797_002315"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ1225076.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5349..5477
                     /locus_tag="IW797_002316"
     CDS             5349..5477
                     /locus_tag="IW797_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1225077.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5481..6026
                     /locus_tag="IW797_002317"
     CDS             5481..6026
                     /locus_tag="IW797_002317"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ1225078.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6480..8171)
                     /locus_tag="IW797_002318"
     CDS             complement(6480..8171)
                     /locus_tag="IW797_002318"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ1225079.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
BASE COUNT         2350 a         1756 c         1554 g         2852 t
ORIGIN      
        1 tgatccgccc ctctgttttt atgattttat taaacttcca ataattcttt ttctttatca
       61 gcagcgatct tatctacttc tttgatgctg ttatctgtca atgtttgtac gtctttttct
      121 aatccgcgca aatcatcttc ggtaatatca ccattttttt gttgtttttt atattcatcg
      181 atcgcatcac gacggatgtt acgaatcgcg atttttgcat tttctgcttc ttttttcaca
      241 tctttagcta attcttttct gcgttcttct gtcagttgcg ggataactaa gcgaatgaca
      301 tttccatcat tggttggact gattccgata tcacttgcca taatagcttt ttcaacatct
      361 tgaagaatgc tcttgtcaaa aggtgtgatc attaaaacac gtgcttctgg aatttggatt
      421 gatgccattt ggttcaatgg tgttggcgca ccgtagtagt tcacagtaat acggtccaaa
      481 aggctagcgt tcgcacggcc tgcgcggatt tgcccaagtt cacgttgaag attttgtgct
      541 gcttttttca ttttttcttt tgcttctgtc ataattgcat ctgccatttt tatttccccc
      601 ttacagttgt tccgatattt tcgcctaaga ttgcacggcg aatatttccg tgttcattta
      661 aattgaagac aagcagtgga atatcattat ccatgcttag tgaacttgca gttgaatcca
      721 tgacttgtaa tcctttggca ataacttcca gatgagtcag ttcttcaaat tttactgcat
      781 ttgcatctac tttcggatca gcagaataaa caccgtctac attgttttta gccatcagga
      841 taacatctgc gccgatttct gccgcgcgta atgctgctgt tgtgtctgtc gagaagtacg
      901 ggttgccggt accgcctgca aagataacga tccgtccttt ttctaaatga cgttctgctt
      961 tacggcgaat atatggctct gcgatttgac gcatttcaat cgaagtttgc acgcgtgtcg
     1021 gtacacccac gttttccaac gtatcctgta aagctaaggc attcataact gtagctaaca
     1081 tacccatgta atcagcttga gcacgttcca tgcccatttg tgctccgatt tgtccgcgcc
     1141 agatatttcc gccgcccaca acgattgcca tttcaacgcc cagctcgtga acttctttga
     1201 tttcttggat gatttctttg attactggtg gtttgattcc aaatcctgcg tccccagcta
     1261 aagcttctcc actcaatttt aagactacac gctgatattt cggtgtgacc atgttcattc
     1321 ctccaatatt ttctattgcc attttaccat acttagatga tttcgacact ctaagactgg
     1381 tttttcttgt tttgcttaaa aaaagggagc gcacaaaccg tgcgttcccg tctgttattc
     1441 aacagaatta ttttttcact tggctcataa cttcttctac aaagttatct tcgcgttttt
     1501 cgataccttc gccaacttcg aaacgtacga atgttttaac agttgcgcct tttgatgcaa
     1561 cgtatttttc aacagtcata tcaggatctt taacgaatgg ttggtccacc aaagcgattt
     1621 ctgctttgaa tttcttcaag cgtccttcta ccattttttc aacgatattc gctggtttgc
     1681 cttcgttcaa tgcttgttct gtaagaacag ttttttcatg ttctaattca gcttctggaa
     1741 tttgtgattc gtttacataa cgaggattga tagctgctac gtgcatagct acgtctcttg
     1801 caactgtttc gtcagttgtt ccatctagaa cagtcaatac ggcaatacgt ccgcccatat
     1861 gcaagtatcc accaaatgca gcattgtcgt ctttttctac aacttcaaag cgacggaagc
     1921 tgattttttc tccgataact tgagttgctt cgatcaagtc agattcaatt gtacctttat
     1981 ctgttttgat tttcattgca gcttccatgt ctgctggttt gttttcagca acaagttctg
     2041 caatttcttt tactaaatct tggaacattt cgtttttaga aacaaagtct gtttctgagt
     2101 ttacttcaac gattgctgca gtgttacctt tcacagcaac tgaagccaat ccttcagctg
     2161 caatgcggtc atttttcttc gcagcctttg ccattccttt ttcacgtaaa agatcaactg
     2221 ctttttccat gtcgccttct acttcaacta atgctttttt cgcatccatc attcctacgc
     2281 cagtcatttc gcgtagttct tttaccattt tagctgtaac gtctgccatg tttaactcct
     2341 cctgtgtgtt tttcttttta aaaaaagctg cttcaaagga gagtgaggct tgtcgcccag
     2401 cctttgaaac agctccatca ttcaaaatta ttctgctgaa gaattgtcgc cttcaacaac
     2461 atctacgatt tcttcgattg atgttgctgt gttttcttct gtgaaatctt cttcaactac
     2521 ttgatcttca ccttggtttc cttcgatgaa agcatcagcc atttttgaag taatcaattt
     2581 aacggcacga attgcatcgt cgtttgaagg gattactacg tcgatttcat ctggatcgca
     2641 gtttgtatca accatagcta cgattgggat atttaatttg tgtgcttctt gaacagcaat
     2701 acgttcttta cgtgggtcaa cgatgtacat tacatctggg attcttggca tatcagcgat
     2761 accgcctaag aatttttcaa gacgttcacg ttctttgttc aaaccagcaa cttctttttt
     2821 aggtagaact tcaaaagttc catcttcttc cattgcattg atttgtttca aacggctgat
     2881 acgtttttgg atagtatccc agtttgtcaa tgttccgcct aaccaacgat ggttaacgaa
     2941 gtattgtcct gcacgagttg cttcttcttt gattgcttct tgtgcttgtt tttttgtacc
     3001 tacgaataat gctacgccgc cttcttcagc aacgctcttc atgtaatcat aagctgcatc
     3061 tactaattta actgtttttt gcaagtcaat gatgtagatt ccgtttcttt ctgtgaagat
     3121 atatttcttc atttttggat tccagcgacg agtttggtga ccaaagtgta cgccggcttc
     3181 tagtaattgt ttcatagaaa ttactgccat gtttgtttcc tccgatttgg ttttattttt
     3241 cctcttctta tcttctacct tgccggcaaa ctttttcaag cacctaccga caatccaata
     3301 agaatgtgga tttgctgatg cataaagcac cctcagctat tatacagaca aaagatccgt
     3361 atcgcaagag caaatatact tttctgctca acattcaata gtttacctga aagataaaaa
     3421 gaattcaaga aaaacagtag atttctcttt gtttctttat tatccttttt ccataaaata
     3481 attttaggta taaaagacga atattattgg gcaatccttc ttttttttgt tatgatacat
     3541 gtgaaatctt tcgtaaagga gaaagaaaat gcgcaaagct caaagacatt tgctgattaa
     3601 acaagtcatt gaagaattcc cgatccgtac gcaagaagaa ctattaaaca aacttgggca
     3661 ttatggcgtt accgccacgc aggcaacgat ttctcgagat atccgtgatc taaaaatcgt
     3721 gaaggcacct gatgaaaatg gcgtttcccg cttcgtattg tttcgcgaaa aaggaactcc
     3781 tgagactagg actgaggaag aggatcggct ggttcaaatg attgaagaca ttgtattgaa
     3841 agtcgaacga gtccactttt tgacgatcgt caataccttg ccagacaatg ctcatttatt
     3901 tgcatctgtt ttagatgaga tcaaacctcc ctctattgtc agtacaattg ctggattcga
     3961 tacgatcatg gtcatctcgg aaaatgagga ggacgcaaaa cgagtagaag aatttcttca
     4021 ccatccaact gaagtatcac ttttttaatc agagcacgta ttcctaatat ggaatgcgtg
     4081 tttttttatt ttcaatattt tccttattga ataaaccttt acactcttta caaaaataca
     4141 tcatttttta ttcaaatttt tcaacacctt gattgaatca caataatcat catttatatt
     4201 ggatgttaac aagagcagct tcttgctctt tgtgtatttc aaaaaagaaa tgaaaggaaa
     4261 tcatatgtat aaattattaa cttctggacc attgacgact tctgaaacag tgaaaaatgc
     4321 tatgctgatc gatcattgta cttgggatca agaatacaaa gaaattacac aatggataag
     4381 aaaaaaatta ttggatcttg ctcaagtatc ggaaggaata tacacaacgg tcttaatgca
     4441 aggtagcagc tccttcgggg tagaatcggt attaagttct gtgatcaggc cagaagatac
     4501 tgtactgatt tgttcaaacg gcgcatatag acaacgaatg atcaacatgt gcgaatgtct
     4561 gaaactgaac tataccgttt atgaggtatc ggaacatcaa atcctccaag ctcatgtgat
     4621 tacacagctg attgaagaga atccgtctat ctccgcacta gcaatggtcc atagcgagac
     4681 tacttccggt attttgaata atttggaaga gattgctgct gtcacacaag aaagagatct
     4741 tttgtttata gtggacgcga tgtcaagctt tggtgggata ccgatttgtg tagccgaact
     4801 agggattgat tttttgattt ccagctcaaa caaatgtatt caaggtgttc caggcttttc
     4861 ttttgtactt gctaataaag cggaactgga aaaacgaaaa aatgttagcc gaagtttgtc
     4921 acttgatcta tatgaccagt accgcgtgat ggaaaacgag aatggcaaat ggcgtttcac
     4981 ttcccctacc catacggttt tggcttttca acaagcgctg aaggaactag aggcagaagg
     5041 aggaatcgaa actcgatatt cacgttatct cgccaataat catgtgctac gacaaaaaat
     5101 gacagagatg gggttccagc ctttcattca agaatatcag gggccattca tcacctcttt
     5161 tctttaccct aaggactggc gatttgattt taatcatttt tatcagtatt taaagcaaaa
     5221 tggatttgtg atctatcccg ggaaaatcag cgatcatgaa tgttttcgta tcggaaatat
     5281 tggcgagatc tatccggaag atattttgga actgacagca attatcaaag actataagga
     5341 gaacgttaat gattaaaact gtaatttttg attgggcagg aacaactgtg gactttggct
     5401 gtatggctcc tgtccacgct tttagaaatg cttttctaga aaaaggaacc caactgactg
     5461 acaaagaaat ccgctaaccg atgggaaaat taaaatggga tcatatccaa cagcttcttt
     5521 ccctcccttc tgtcaaaaaa caatggatag ctatttatgg acaactacct caggaaaaag
     5581 aggtggagga attgtatcaa caatttgaac attatctgtt taaagatttg gtcaagcatt
     5641 ctaccttgaa acccgacaca ttggaaacga tcgaacggtt gagaaacgct gggatcaata
     5701 ttggttccac gaatggattt aatgctccta tgatgacaat cgtagcagaa accgcaaaaa
     5761 aagaaaggta ttcaccagac ttcatggcaa cttttgaaga cgtcgaaggg tatggtcgac
     5821 cgtttcctta tatgatccat aaaaacatgc agtacttcaa aaacaagtcg gttgatgaag
     5881 taatcaaagt cggagatacg gtctcagata tccaggaagg gaaaaatgcc ggagtgttga
     5941 cagtaggtgt aattgaaggt agttcgctaa tggggatttc ttatgatgag taccaaaaat
     6001 tagagatggg gaaaaagatc gtttaaaaca gtctgttgct cttagattcc aacaagccgg
     6061 cgctgatatt tgtgtaaaca atttaaaaga gttaacagaa tacattttgt acgctgacaa
     6121 gaatccagaa tgacttttat acaaaataag aggttgtgaa aaagctgtcc tgtatcaagt
     6181 aataaaaaca gccaagcaaa aatagctcct catatttttc gtttggttag gcttattacc
     6241 gcagatgcaa gcttttgaac accgtttatt cgaaaaaaaa aagggagctg tgacacgact
     6301 tttgtcacag tccccttatt tgcaaacaaa cagtgtttaa gagacaacct ctccactaaa
     6361 cggtgttcat tcagctgact ctcggtattt gctcaaaaaa cgataaaatt tgaaaagcta
     6421 ttttctcttt tgtttctcta atactcggat cagaacgctg atttcacatt ctctttttct
     6481 tacatcttat ctggtgcatg aagtcctagc aaacgaaggt cttctttcaa taatacagtc
     6541 actgcataaa ctagagctaa gcgtgcttct ttttgttcat catcagctaa aatcttcgta
     6601 tgagcatagt atttgttgaa tgcttgtgct aatttgattg catgtttcgc aatcactgac
     6661 ggttcgtatt tttccccagc agacaagacc gtttcagggt atttttgaac taatttaacc
     6721 acttcccagc tggtatcgtc attcaatgca tagtctgctg tttctgaagg gacaaatcct
     6781 gctttttcca acaggctgac tgcacgagca tgtgtatatt gcacataagg tccagtttct
     6841 ccttcaaatc gaacaacttc ttccaggtta aagtcaaatg tattcagtcg atcgtttttc
     6901 aaatcgtgga aaatgactgc cccaacccca acttgtttag ctacagcatc tttgttttcc
     6961 aaatcagggt ttttctcgct gatttgttct ttagcagatt ggattgcttc atttaatact
     7021 tcttctaata agacgatctt tcctttacga gttgataatt ttttaccacc ttgcgtgatc
     7081 aaaccaaatg gaatatggtg catgtcgtca gaccagtcaa agcccatttc cttcaagaca
     7141 gctttcaact gtttaaaatg atagctttgt tcattcccta ctacatatag agattgtttg
     7201 aagtcgtaag tacgtttacg gtacaatgca gcagctaagt cacgtgtaat gtatagtgtc
     7261 gcaccgtctg acttcttaat caacgctgga ttcaggtcat aagcagatag gtcaacgatc
     7321 tctgcacctt tatcctcgtt cagcaaatgt ttttcttcta aaagtttgac gatttcatcc
     7381 atcttgtcat tgtagaaagc ttcaccgttc aacgaatcga aacgtacttc cagcagatca
     7441 tagattttat tgaattcttt catcgattca tcacggaacc attgccataa ttgaatagcc
     7501 tctttatcgc cttcttccag acgtttgaac caagcacgag cttcttcatt taattctggt
     7561 tctgtttcag ctacttcatg gaattggacg tataaacgaa gcaattcatt gattggttct
     7621 gctttaacag cttcttctgt accccatttt ttgtaagcga cgatcagttt tccaaactgt
     7681 gttccccagt cacctaaatg gttgatgcgg attggctgat aaccgatttt ttccataatg
     7741 aaaccgatcg aattaccgat aactgtcgaa cgcaaatgtc ccattgagat tggtttggca
     7801 atattaggag aagacatgtc gattggtact gttccttgat tcccgatgtt gctatcccca
     7861 taatgttctt tttctttcac tactgtttgc aaaacttttt tgctgataag ttctttattc
     7921 atgaaaaagt tcaagtaagg tccgacaact tcgatttttt caaagttggc agaatcgatt
     7981 ttttctgcta aatctgcagc gatttgctgt ggcgcttttc ggtaaacttt tgctaatgaa
     8041 aaggctggaa atgcaacatc tccgtgttca gctgattttg gattttctag taattgctcg
     8101 acttgttcca gtgttaaatc atctttgaca gcgtcataga cagcttttgc gacaagatct
     8161 ttattattca tctttattcc tcctaaagtg ttcccttgat tgttaaacaa aaaaaatctc
     8221 tagcgacggc atacttagta tgccatcact agagacgaga tttcccgcgg taccactcca
     8281 attgtcctaa aaaaggacca gctccatctg atatcggtag acgaccgtct gtttaaaaca
     8341 gaattctcca agtgcgcttc gttgtatctt cttcattcgg cttccaccac ttccgaattc
     8401 gctagccaag aatgatacaa gtactctctt gttcaacaaa tcttagtctt attaaacttt
     8461 cttgaattat atcagaaaaa aaaacgaaag gctagtcgtt tcataaaaaa aa
//
LOCUS       DACEPD010000095         8493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.099, whole genome shotgun sequence.
ACCESSION   DACEPD010000095 DACEPD010000000
VERSION     DACEPD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.099"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..173)
                     /locus_tag="IW797_002319"
     CDS             complement(<1..173)
                     /locus_tag="IW797_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1225080.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IW797_002320"
     CDS             complement(593..1714)
                     /locus_tag="IW797_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAQ1225081.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /locus_tag="IW797_002321"
     CDS             2042..3451
                     /locus_tag="IW797_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ1225082.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNRLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IW797_002322"
     CDS             3448..4176
                     /locus_tag="IW797_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ1225083.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IW797_002323"
     CDS             complement(4304..5215)
                     /locus_tag="IW797_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1225084.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IW797_002324"
     CDS             complement(5232..6239)
                     /locus_tag="IW797_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ1225085.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IW797_002325"
     CDS             complement(6236..8365)
                     /locus_tag="IW797_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ1225086.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IW797_002326"
     CDS             complement(8370..8477)
                     /locus_tag="IW797_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225087.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1726 c         1486 g         2682 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcgc ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DACEPD010000096         8425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.100, whole genome shotgun sequence.
ACCESSION   DACEPD010000096 DACEPD010000000
VERSION     DACEPD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.100"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            471..2108
                     /locus_tag="IW797_002327"
     CDS             471..2108
                     /locus_tag="IW797_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ1225088.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2253..2993)
                     /gene="truA"
                     /locus_tag="IW797_002328"
     CDS             complement(2253..2993)
                     /gene="truA"
                     /locus_tag="IW797_002328"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ1225089.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3184..3819)
                     /locus_tag="IW797_002329"
     CDS             complement(3184..3819)
                     /locus_tag="IW797_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ1225090.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="IW797_002330"
     CDS             complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="IW797_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ1225091.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5405..6401
                     /locus_tag="IW797_002331"
                     /pseudo
     CDS             5405..6401
                     /locus_tag="IW797_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6417..>8425
                     /locus_tag="IW797_002332"
     CDS             6417..>8425
                     /locus_tag="IW797_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ1225092.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2785 a         1547 c         1293 g         2800 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctatggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacacat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct tctttattta
     1501 tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat aattcatgga
     1561 aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta agcaattcta
     1621 tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt gctatagctg
     1681 aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct gatatgatta
     1741 ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat tttatgagta
     1801 acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg acgacaccta
     1861 tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa acaaaattat
     1921 ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc gttattactt
     1981 atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact ttgattttat
     2041 ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa gtacaacatt
     2101 ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag gctaaaaagt
     2161 ctttttttat atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg
     2221 aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac
     2281 agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca
     2341 tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc
     2401 cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt
     2461 cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca
     2521 gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc
     2581 atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg
     2641 ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc
     2701 tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag
     2761 tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc
     2821 gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc
     2881 tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc
     2941 catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag
     3001 tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata
     3061 tagctccttt cttctctaaa acagaagcta tacaagaaaa ctgtggaaat atatatttca
     3121 gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa
     3181 gttttactgt ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa
     3241 actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat
     3301 tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa
     3361 gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa
     3421 taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca
     3481 cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata
     3541 gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactta caaatgcagc
     3601 atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa
     3661 cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc
     3721 taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa
     3781 taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt
     3841 acctatgctt ttaattgtta ttataagcga ctttatcttg atttaacaga aacctctatt
     3901 tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct
     3961 aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt
     4021 cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag
     4081 gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc
     4141 gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg
     4201 cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc
     4261 aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg
     4321 gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac
     4381 tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa
     4441 cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag
     4501 ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc
     4561 atcgtaccga tcaaaacaag taaaagctgc agatactctg ccaatccagc tattccagtc
     4621 gtagcaattg attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact
     4681 tgtacgactt gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa
     4741 cgtgctcgaa aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca
     4801 tcaataagag attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt
     4861 tccaaacttt gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa
     4921 attgaactta caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc
     4981 aaaataggct tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc
     5041 aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat
     5101 attgaaataa atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca
     5161 acaacaatac gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt
     5221 cacaatttta acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa
     5281 aaattctaac aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt
     5341 tgttttttga gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt
     5401 tcttatgttt ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga
     5461 gagttatggc ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt
     5521 gatttcagca gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac
     5581 atttttacaa acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca
     5641 atatcaaaaa atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt
     5701 cctaatagcc acactagttt gcaatatctt ttttttacgt acaagctggc agcttgaata
     5761 ttcgtgatta ttggcttatc ctaacaccta tcagtcagaa ttactttact tatgctgttt
     5821 cttgtacttt gcttttttgt agtattcctt atacagttgg atttttaaat aaattatcga
     5881 aaacaacaat taaatatcta ctattatttt taacaattgg attagttggc tgctctactc
     5941 tatttaataa agacccgttt gcattacaga atggacagag tattttatgg attttttatt
     6001 tatttttaat gggttatggt ttgaaagaat ggaactggaa agaaaaggtc cgctataagc
     6061 ttcctcaagt atttatcagc ttgattgttt tattcggttt gattattttg atgacacaaa
     6121 tttcactcat catcagagga aatacagata cagcgctgag gttttctaaa ccatattctc
     6181 tcttcgcaat gtattatact ttctcttcgt ttttattttt agaaatgcta tctgataaat
     6241 taggtataaa aggaagagga aattttcttg ctgtattttt gattgtcact caagttatat
     6301 gtaatttccc tttagtttct tatcgcgtat ctacatttat gaaacgtgaa tttccaaact
     6361 cagggaaggc atggctgata aatatcgggc agttttttat ttgctattta ttagcagtgt
     6421 ccctcctagt tatcttttta ttttatatac aaaaaatccg tttcattcat cgtttcatcg
     6481 aatacttcgc ttttgattcg attgaacaat taactcagcg ttggaaaaaa agaattcatt
     6541 ggctttttgt tcataaaagt tattttttag ttgcagcatt tttttactgc tttaccttcg
     6601 tgcagatttt cgttttagaa ccaaaagaag gttggaaaga aactatccag gttgccttaa
     6661 aaatatttac tcagcgacaa gcacccatga tcctgacaat catcattatt atgggatttt
     6721 tcttcttact attgttgatt tccaatcgtt tttggtacgc cttgactgct accttgatca
     6781 ttaacttact gctcactatt tcgacagtaa tcaaaatgga aatgcgggaa gagcctgtct
     6841 ttccttcaga tttaaaaata ctgaccggat tgtcagaact attatcgatg gttagcccag
     6901 tcttattaat cgtgggcgga ttgatccttc tcctattatt gattactagt attattgtac
     6961 aacgtagact acaaaaacag tattccttga agattcattg gaaacgtcga tttatcagta
     7021 tcgctgtatt attaggatgt ttttcgggtg tctttttcat taatcacaaa aattcgcctt
     7081 ctttcttgct gtttaattta tttaaagtaa acaagacttt ctttaaccag caaggcgccg
     7141 taaaagataa cggaccagtc attcaattcc taaacaatat cgacattaaa atcatggaag
     7201 aacctgctgg ctattcgaaa gaaaaaatta atggaataat gaaaaagtac aacacaaagg
     7261 cagatgaaat caaccaaaca agaagtgact ggttaaacaa tcagacgatc gttctcaatt
     7321 taagtgaaag cttcagtgat cctgcacgtg tccctaatct gacagtacct acagacccta
     7381 ttccttacat caaaagcatt ataacagaag gaacaggagg attgatgtta tccgtcggtt
     7441 atggtggcgg aacagctaac atggaatggg aaggactgac aggcttgagt atcagtaacc
     7501 tgtctgcttc gttagttaca ccttataccc agctagtgga gcgtcagaaa ataagtccaa
     7561 atattaccga tctattcgat gaaaaaatag ccatccatcc ttttacagct gctctttatc
     7621 gcagaaaaca agtgtttgat aaatttggtt ttgacaggtt ctattattta gacagtccgg
     7681 ataaattaac ttatacggat aaaatagaac aaagcccacg catatcggat gattcagcat
     7741 acaaagaaac cttgaaagct ttacaaagca atacagaaac gtcacaattc atccaattat
     7801 ccactatgca aaaccatatg ccttatgatg attactatag tgaattgaac tacactgctg
     7861 aaggaactgc tgtcattcct aatagaagaa aagaattaca aacttatatg caaggccttc
     7921 actataccga tacagcagta aaaaaattca tccaagaaat cgacaaaatc gataaaccga
     7981 ttacctttgt tttttatggt gaccatttac ccgcaatcta tagtgggaat gattcgaaaa
     8041 aatatggatt agaacagcac cagacagatt atttcattta taacaacaag tatagtcgtg
     8101 aacaagcagc tacgttaaat aaagaagttg ttgctcctaa taactttggt gctatggctc
     8161 ttgagcatgc gaatatcaaa gtaactcctt actatgcgtt gttgacagaa gtatctaatc
     8221 atctacctgc attaacaatt gatcctacag aaagcttgtc taatcgtttt aatggaaaac
     8281 aggtctttgt tacagaggat agtcaaatta tccaagaaaa aaatttaact gagaaacaaa
     8341 aagaaattct agaagattat cgtctgatac aatatgatct tgttgcaggt aaccagtaca
     8401 gtgcaacatg ggcgacacaa aaaat
//
LOCUS       DACEPD010000097         8415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.101, whole genome shotgun sequence.
ACCESSION   DACEPD010000097 DACEPD010000000
VERSION     DACEPD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.101"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            31..1332
                     /locus_tag="IW797_002333"
     CDS             31..1332
                     /locus_tag="IW797_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1225093.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(1403..1966)
                     /locus_tag="IW797_002334"
     CDS             complement(1403..1966)
                     /locus_tag="IW797_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1225094.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(2021..2200)
                     /locus_tag="IW797_002335"
     CDS             complement(2021..2200)
                     /locus_tag="IW797_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225095.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(2277..2786)
                     /locus_tag="IW797_002336"
     CDS             complement(2277..2786)
                     /locus_tag="IW797_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225096.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(2882..3262)
                     /locus_tag="IW797_002337"
     CDS             complement(2882..3262)
                     /locus_tag="IW797_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ1225097.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            3592..4341
                     /locus_tag="IW797_002338"
     CDS             3592..4341
                     /locus_tag="IW797_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ1225098.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(4525..5802)
                     /locus_tag="IW797_002339"
     CDS             complement(4525..5802)
                     /locus_tag="IW797_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225099.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(5881..6525)
                     /gene="pcp"
                     /locus_tag="IW797_002340"
     CDS             complement(5881..6525)
                     /gene="pcp"
                     /locus_tag="IW797_002340"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ1225100.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(6724..7668)
                     /locus_tag="IW797_002341"
     CDS             complement(6724..7668)
                     /locus_tag="IW797_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ1225101.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(7670..8356)
                     /locus_tag="IW797_002342"
     CDS             complement(7670..8356)
                     /locus_tag="IW797_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ1225102.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         2463 a         1765 c         1450 g         2737 t
ORIGIN      
        1 caccacaaca caatcaaagg agcgttctgt atgcctgatc ttcatcatat caaactacta
       61 cttggaatca aagataaaaa cattttcatc acaaatgtgg aatcgaagag cattaagaat
      121 atcaagagtc ttgttgtttc tgcgacgtta agtaaagaaa ttcgtcgctg tcctttgtgt
      181 aaacagatga atcatgaagg catgatcgtt aagaacggaa agaaaaaatc gctcattcaa
      241 cttaataaat gtgcgaacca actgacttat cttgctttag cgaaacaacg ctaccactgt
      301 cgtggctgcc acacctattt tacggcgaat acctacattg tcgatagaaa ctgctttatc
      361 gctaaacaag tgcgctataa aattttagaa gagctaactg aaaaacaagc aatgacgaca
      421 attgctaagc attgcggcgt ttcttggtcg accgtttctc ggacactcgc ttctctgatc
      481 cccatgacaa aagtaaaaag aaattggtta ccgcgttgtt tattaatgga tgaatttcgt
      541 tcactgaaaa atcaagtcgg tccctactcc ttttcttgta tggatggcga tactggaaaa
      601 ttactggata ttcttccttc acggaagaaa aaggctctcg tttcttattt tatgcaattt
      661 gaacgacgcg cacgattgaa ggtaaaggtc atcgtgacgg acatgaatgt ttcttatgcc
      721 tctctgataa aggaatgttt tccaaaggca aaactcgttg tggatcgttt tcacattgtc
      781 aaacatctca ttcgtaaatt tgaggatatt cgcgtacgaa tcatgaaaag ctttgatcga
      841 aacgatccaa cacaagccaa atattatcga caattaaagg cgttgtctcg gcttctgatc
      901 aaacgacagg acaagcttgt ttatgacaaa tggaccaaat ggcgcaattt tggttgggcg
      961 tatctcaccg aaagtgaagt ggtggatcgt ctgctttcga tctctgacga attacgaatc
     1021 gcctattttt actatcaaga aattcttcaa gcgtttcacg acaaagaggc ggacactttc
     1081 tttaaacttg taagaacgat gcccaattcc gttccaaaag aactgcattc tataaaaaaa
     1141 gcttttataa actatgaatc cggcattcgt ttagccctcg aattgcctta ttcaaatgcc
     1201 aaaatagaaa atctgcatac ccacatcaaa gcattgaaac gagtcgctta tggctttcga
     1261 agctttcgga aaatgaagac cagaattttc ttactaaata atctgattac ctatgaatca
     1321 aaaaacatct aaagtagaaa aggaattcta ccttagatgt tcttatctca ctctatcagt
     1381 cctatttgac aaagagccct ttttagatat tcttttgcat atgatcataa agatgaccac
     1441 tgatcatccg ctgaccgaca actttgaagt catggcgttc gtataatttt ttagcctttg
     1501 gatttttttc atccacactt agaccgatga cggagcgatt agctttcttc gccaatttag
     1561 gtaatgcttc taataatcgc gaaccgatgc cttgtcctct aaaatcttct gatacactga
     1621 tagagtccaa ataccattcg ttgggaaacg tttctttatc aatgaacaaa cgtgtagacg
     1681 catctagctg atatttctct aagataggta ccagtggctg atcgatgatt gattcttctt
     1741 catctgtata accgtaagca actcctgcaa ctttaccgtc tatttcttct acgatacctc
     1801 gtgaataact gtaacgataa tttggatcag tgattgcttc ttctaaaatc tctaaagtct
     1861 tttgttcccc tgtctgcttc aaaaatggca gttccatatc ttttagaatc actaagatca
     1921 atggggcgat ttctggagca tcttcttttc tcgcaaaacg aatcatagtt tcctctcctt
     1981 ttctccattc taacatgcag aaaaagaaat cgcaaaatcg ttattttaaa aaatcttcaa
     2041 tcttttcagt aatgaaataa ccatgagtaa cgattcttgc cgcttcagtt cggcaataag
     2101 ggcagacgac tggcatttct ttatcattat gttcgactac attattgact tcttcgtatg
     2161 caaatttttt tccgcaatta gggcatttca ttttttccat gttatctcaa cttcctttca
     2221 tctttactct cttagcttac tcttatattg taaaaaagag aagcaatatc cttcttctat
     2281 gcagatatcc atacctgacg gtcctgttct ccatctatta atcggacttt ataataagga
     2341 cgttcatctt tgactagaag tgtccactct ttcacttttg cttttttccc gtagtagctg
     2401 actgcttcct tcatcgctgc ttgcggttgt ttgaactctt ccgttttttc tatatcaaaa
     2461 agatgttctg cggccttttc cgtgtcattt cgtacggttg ttttcccagt ggatggattg
     2521 actgctatga cttgtccttc acaaaaaaac tggtattcat atgttgcatt cgtaggattc
     2581 tccaaatctt tttcaactgc aaaatgaata gcagatatgt tatgttcttt tgtttcgttt
     2641 tttacgattg ctgctgcttt tttcaatgag atccgatatt cctggtcact aatatcatcc
     2701 atgtgaaaag cggcattgag cagtcccgtc ataccggtaa tccctattac gatgagggca
     2761 gcaacaccaa acatcatttt tttcattctt cattctcctg tttctttttt ttaaagaata
     2821 tcaagaaatg aacgaatgaa aaatcgaatg aaacgaaaaa aaagtgacaa ttttgttatc
     2881 tttattcgtg cagttctaat ggcaatccgt ctggatcaaa gaaaaacgtc attttttttc
     2941 ctgtaaaggt atcttctcta atcggttctg attcgatccc taaggactgc aattctgcaa
     3001 ttgtttcttc aatatcgtct actttgaaag caagatgacg caagccacaa gcttctggat
     3061 agtttggtcg ttgtggcgca ttagcaattc caaaaatctc caattcagca cttcctagtt
     3121 tcagatctaa tttatagtca tctcgttctt tgcggtaatt ttctctgatt acttcaaatc
     3181 ctaatttatt aacataaaaa tcacgtgatt tctgataatc tgaaacaatg atggctatat
     3241 gatggatcgt tgtaaggttc atatgagcac tcctattctt cgtttaagct cggatctata
     3301 ttaatctcca gttccattgt aaatgtgtca gattgaaaaa aatagctgtt tttccttttt
     3361 ataagaaaaa aggcaaaaaa aacagaaagt aggtcctcca agacactctt cctgttccca
     3421 atataataaa tgaaagtaag aaattatatt ttcaagaaaa caaacagaaa attatcataa
     3481 cacaaattat ttataattga aagactagtt ttttttcaag aaaagcatac aatagattgt
     3541 gttagtaaat acagaacaca ctacatttag tgtcttaagg agtcctcata catgaagtca
     3601 tattttcagt ttttagctca tcagctaaaa ggctggccgc aacaaaatta ttatcttttc
     3661 tttttcagtc ttggctgcca ggtcatgact cttgtcaata gtcccatcac ttggctgtcc
     3721 gttattacat ttatcggtac cacgcttggt gtattgtgta ttttatccat caatgcggca
     3781 aaatcagtca acggtatttt aggtattttg tctgcgattt gttttattat cgttgggttt
     3841 tcagcaaaga actatttaag tatcggtgaa cagattgctt atatgatcac attggatatc
     3901 cccgtattgt taagtaaaga atggaatcat aatatggctt caaaaatccg aaaattcact
     3961 ggaaaaagct ggtttatcgc aatcatcagt actttccttg tctatcttgt ttcaggattc
     4021 ctgattggtc gttatacaga tgatcctcgg ccgtgggtag atgccattgc tttttctatt
     4081 agcttgactg ctggtatcat cagtttcttg cgttacaaca accaatatta ctggtggtta
     4141 gcttcagggc ttgctcaaat ggtcctttgg tatatttcat ttacgcatgg atctgcaagt
     4201 ttggcgatgt tcgtgaacag ctctgtttac ttgctgaatg atgtccttgc atttaccgtt
     4261 tcaccttggt ataaccaaaa agaacgtgaa cgtatgcaaa aaatggaaga agaatactac
     4321 aacgcacaac aacacgcata aaaaaactga gcgtgggaca aaagtaaaga acacttttgt
     4381 ctcacgctct aaacacgtat aaacggcgga atcagaagca actccttcgg aaataagctg
     4441 aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa
     4501 cgcttctgtc ccagcctcta ttttttatag taattccttc agatactttc ttgcctcatc
     4561 attgaaacgc agtgattctt ctaacttctg ataatattcc aacgcttgtt tctgagcttc
     4621 agtaaaacga taatgttttc ctttccattt gtcgattgtc tcttgcacat ttccagattg
     4681 gatatctttc actactagtt gccatttttc ttttgagact gtacggtctt tcttatcagc
     4741 agattgtata gcttctcgtt ctttacgcaa tacttttaaa aaaccatttc tgactatccg
     4801 aggaatgctg tcgaagaacc cttctgtaaa accatagcga caatattgct tccaaataaa
     4861 tagatagatt tttggttgga tgaatcgttg gtttttcgca aaatattcca tctgagcagt
     4921 gacttctggt gttcgttgac gttttgtctt tctcaacacg gaaataaatg tttcaaaata
     4981 agcattagga tgtactggca cttcttcata tacgatggat tgccaatctt tacgtaaaaa
     5041 tgcgcgatag ttgatcaaga attcttctac atcctttact aattcttctg caaagtcgtc
     5101 aaatagttct ttttttatct gtcgcttttt tgactgccca ttgatgtgtt cacttatttt
     5161 cttgatttca ttttccaatg cttgataatc cgctatgtaa atcggcattt ctacttggcg
     5221 aaattctgcg agccgctgag ctaattttat atccggtaaa tatggctgtt tcatccgacg
     5281 attttggtac aagacaaaac agtatttttg ccatagtcct tcaggaaaat cgatattctt
     5341 ctgcttaagc cataataatt cgtctaaggc cagatcaact tttgtcattc cctgatggat
     5401 cgaagctgcc actgtcggag agacatgcag gtgatcggca aagtggttga tgcctagttt
     5461 taggatcttt ccggtttttg gcgatttaac gatgtattgc acttttaatt ctctaccaca
     5521 ctcacaatac aatttttgct ggcttcgttt tgttcgaaaa aacggattga tcttgaagtc
     5581 agaaaacacc caatctgttg cagctaaata atgttcttcc ataaaacgag agctgattag
     5641 atatcggcgg tgcttatcga ctagttcccg ctgttttttc gttaattttg catagatctc
     5701 ccaacgttga ctgcttgtgt attgtttaga aattttgatg ggatagcttt tgctgatttc
     5761 ttttggaaac cattcttcaa agacggattg ccattcttcc acggctgttt ctttcctttc
     5821 taattacaaa aagctggcta ctaacataac gttagcaacc agccagtgtt tcttgctaaa
     5881 ttagtgagtt gttccggcat ttccggaaat atcttcttta ttttctactg ctgcttcaac
     5941 tgcataagtc aaagaagaag caatgtcttc taaagacata gaaggcgtac ccactggacg
     6001 atcgatcact tgatcgggtg agaatggcac gtggatgaac ccacctcgaa ttgccgggaa
     6061 ttgtgtttcg atcaaatgaa gcaaacgata catgatatca ttgcagacaa atgttcctgc
     6121 tgtataagag atgtacgctg gtagaccatg atcttgtaca ttttttgtca ttgctttgat
     6181 tggcaatgag gtaaagtaag cggctggacc gttttcttct aaagccgtat cgaatggctg
     6241 atttccttca ttatcaggga ttcttgcttc tgctagattg attgcgacac gttcgaacga
     6301 gactgctgac cgcccacctg cttgtccgac acagatcacg acatctggtt ggtgctgttg
     6361 gatggcttct ttgactactt gactgctttt ttcgaatacg gtcggtactt ccactttgat
     6421 gatttcagca ccgctgattt catctggcat tttttttact gcttcatagg ctggattcac
     6481 tttgtcccca ccaaatggat caaaacctgt tactagtact ttcatctgtt gttcctccgt
     6541 ccgattgatt taacatatgg ttatctttta tcataccatt ttccttgaac agcgtaaata
     6601 aaggaaacaa tagtttaaat aacataacag atcaaactta atatattctt ataaaaaaat
     6661 agtgttccac tacaatgtta gaagaggttg cgacaattat tctgtcacaa cccctctctt
     6721 ctattattta aatagaatca tataagcaat ttggaaaact agcataaata acgcaataaa
     6781 caactggttt ttgatcactc cgtatttgtc tttcatctcc agcatcgcta caggcacgat
     6841 attgaagtta gcagccatcg gtgtcagcag ggtcccacaa aaaccgcaag tcaatgcaac
     6901 cattcctatt aacgctggat tcgctccatg actaaatacg aatggtgcac cgatcccaac
     6961 cgtcatcacg gtgatggccg caaatgcatt ccccatgatt accgtaaata tcaccatgcc
     7021 aagagcgtaa atgatgatgc cgatgttcac atttccttct ggaacgatcg tacctactgc
     7081 attggaaatc actgtcccaa ctccagcgct tgtaaatacc gcacctagag aagctaaaag
     7141 cattggcaac atgcttaacg gccctactgt gcccagcata tctgctgcat catctaaaaa
     7201 tgtacttggt cgattatctc gagaaaagaa catcaagatt agcatggctg caaaaacacc
     7261 gacgcccacg ccgactaaag cacctaaatt ggtaaataga gcaaacaaga tcgcaaagac
     7321 accgatactc aaagctggaa taaaaatttt cattcctagt ttgtctgaca ttttttccat
     7381 atattctttt gatggaagcc ggctttctcc tttgctgact ttcttagcaa ttgctggtat
     7441 ggtcatcgca aagatcaagc caccgttcac gattgccgga agccatcttc ctcctgcaat
     7501 aactacccct aagaccgtcc agaaaaaggc tgacccataa cgatgaggat tggttttatc
     7561 tgttaaattt cttactccag tgtaaatcaa tacgagtccc atcacgatat aaaggatctc
     7621 cagtaattta tcgcctaata aaatatcagg atttgtaaaa aagtccatct tattgcccct
     7681 ttcctggata atatttcttc gctaatttct tttctttaag atagaagtag acacttgcca
     7741 caactaaagc cgtgatagcc actggaactt ccactaaagc aagctgtccc aatgttacgt
     7801 catatcctag atctttcagt gttgattgga ctagcaacgc ccctgctcct ccgatgaata
     7861 gtacttggcc aaagaaccag cagacatttt ccatagctga agccatacct tttagttctt
     7921 cttcatgctt aggatggact tctaaccctt ttgattcgat ggtccccaat gccatcggta
     7981 aaatgattgg acgaacaaat ccagcaactc caccaaaact cacgttaaaa gccgcaaaga
     8041 tcattcgaaa gatcccataa atatctacaa tcataccagc agatactttc ttgaatcgct
     8101 tgatcaagga agccgctgat tctttcagac catttctttc aagcgttcct gttgccaaca
     8161 taatgatgat aaaaatcgcc atcccgcgat tgtttacaaa acttgtacct aaaagttcta
     8221 acattccttg aatgccaaga ccgccagtca aagctgtaac aactaaagca accattacga
     8281 tcaatatcga gtctaattta aatgcaaacc cgagaataac aatcaagacg cctaaaaggc
     8341 taagataatt ttccatatat tttcctccat aaaatgacaa tattttttta attataaagc
     8401 aaaacacata aaaaa
//
LOCUS       DACEPD010000098         8404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.102, whole genome shotgun sequence.
ACCESSION   DACEPD010000098 DACEPD010000000
VERSION     DACEPD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.102"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..189
                     /locus_tag="IW797_002343"
     CDS             <1..189
                     /locus_tag="IW797_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ1225103.1"
                     /translation="NSNGILRRNGLPKSMDFREVNQTFISSVSNQRNYIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            326..1264
                     /locus_tag="IW797_002344"
     CDS             326..1264
                     /locus_tag="IW797_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ1225104.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            1279..2109
                     /locus_tag="IW797_002345"
     CDS             1279..2109
                     /locus_tag="IW797_002345"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ1225105.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLLVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            2153..3178
                     /locus_tag="IW797_002346"
     CDS             2153..3178
                     /locus_tag="IW797_002346"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ1225106.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNHYFRIYTNPDVVGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            3193..4131
                     /gene="galU"
                     /locus_tag="IW797_002347"
     CDS             3193..4131
                     /gene="galU"
                     /locus_tag="IW797_002347"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ1225107.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKIELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            4257..4697
                     /locus_tag="IW797_002348"
     CDS             4257..4697
                     /locus_tag="IW797_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ1225108.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIIRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            4700..5302
                     /locus_tag="IW797_002349"
     CDS             4700..5302
                     /locus_tag="IW797_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1225109.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSAELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(5364..6467)
                     /locus_tag="IW797_002350"
     CDS             complement(5364..6467)
                     /locus_tag="IW797_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ1225110.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGADSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVVWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            6677..7678
                     /gene="ccpA"
                     /locus_tag="IW797_002351"
     CDS             6677..7678
                     /gene="ccpA"
                     /locus_tag="IW797_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ1225111.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            8032..>8404
                     /locus_tag="IW797_002352"
     CDS             8032..>8404
                     /locus_tag="IW797_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225112.1"
                     /translation="MPKKRRKTIVIILTILAIGIGGKFYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGCYGALVTMTNNEGKSVYFDYFFSKQMNEISSY
                     GVENEEIQNEGVTTPKIRVIYS"
BASE COUNT         2844 a         1491 c         1802 g         2267 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatggattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgtaatt atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata taattaagaa tgcgttttct attacattgg taattaaata
      241 aaaacgtttt ttttaattgg tagtactttt ggctagacaa tgataaaatg gtagagaaca
      301 ctctaataga aatgaggtag catgaatgtc agaagttgta aaagttagcc aattagtaaa
      361 aaaattatct ttggaaatcg tcacaggcga cgagaaaagt ttagaccgcg tcatcgtgac
      421 tggggacatc tcacgcccag gactagagtt gaccggttat tttaattatt attctcataa
      481 tcgtttacag ctatttggca gtaaagaaat cagttttgcg gaacggatga tcccagaaga
      541 gcgtttgatc atcatgagaa gattatgtag tgaagatatg cccgcattta ttatttcaag
      601 aggattgccg gctccggaag aaatgatcca agcagccaat gaaaatggga tagctgtgct
      661 gcgttcgcct atctcaacgt ctcggctgct aggtgaatta tctagttatt tagatgggcg
      721 cttagcccct cgaacaagtg tccacggtgt gttagtcgat gtctacggat taggtgtact
      781 aattcaaggg gacagtggga tagggaaaag tgaaacggcg ttggaactga ttaagcgcgg
      841 acatcgattg atcgcagacg atcgagtaga tgtctatcag caagatgaac tgacggtaat
      901 cggagaacct cctaaaatcc tggaacatct gattgagatt cgagggatcg ggatcataga
      961 tgtaatgaat cttttcggtg cgagtgcggt acgaggctct atgcaagtcc agttagctgt
     1021 ttatctagaa gcatgggcga aagataagaa atacgaccgt ttgggttctg aagacacgac
     1081 cgttgaaatc gcggaagtgg gcattccaca aataaaaatc cctgtcaaaa caggacgaaa
     1141 cgttgcaatc atcattgaag tagccgcgat gaatttccgc gcgcgtacaa tgggctttga
     1201 cgcaacgaaa acatttgaag aaagattaag tcgcttgatt gaagaaaact caggcaatga
     1261 ctagcaagga gtgacaatat gttagcacaa atcaatcgag ttgcttttga gcttgtagga
     1321 attcctatct actggtatgc attgattatc gtatcaggaa tcattattgc catgtggctc
     1381 agcagcagag aagctgttcg ggtaggatta aaagcagatg acgtgacaga ttttatgttg
     1441 tggggattgc cgatttccat tattggtgcc cgtttgtatt atgttttatt cgatcttccc
     1501 caatatatag ctgatccaat ccaaatattc aatatccgtt caggaggatt agcgatttac
     1561 gggggattga ttgcaggtgc aatcacactg tactttttta ccaaatatca ttttatttct
     1621 ttgtggacct ttttagatat cgctgcacca agtgtattac tggcgcaggc aatcggacga
     1681 tggggaaatt tcatgaacca cgaagcgtat ggtccagaca cgacacgaag tttcttagaa
     1741 agtctgcatt taccgcaatt catcattgat aacatgtata tcgaagggat ctatcgacaa
     1801 ccaacctttc tttatgaatc actttggagt ttgattggat ttattctgct tttggttttg
     1861 cggaagaaac cgcatctttt gaaaaaagga gaagtccttt tatgttatct aatgtggtat
     1921 agtttcggac gtttctttat cgaaggatta cgcacagaca gcctttactt tttaggtgtg
     1981 atccgcattt ctcaattatt gtctgctgtt ttacttgtcg gcacattgat tctgtttatt
     2041 tggcgtagaa aaaaaggaaa tttaccggat tatgaccgct cattaggaaa aaatcaacaa
     2101 attatttgat ttttagagcg ttaaaaatga aaaaaatttc aggagtggta aaatgaatta
     2161 tagagtagcg gttttaggac caggctcttg ggggactgct ttagcacaag tcctcgctga
     2221 aaacggacat gacgttcgga tttggggaca tcgtgcttca caagtagaag aaatcaacac
     2281 ccaacacacc aatcaacgtt atttaccaga tcatcatcta cccgattcca tccgagcatt
     2341 caccgatatg gaagatgcag taaaagatgc agatgcggta ctattcgtta tcccgacgaa
     2401 agcgatacgt tctgttgcaa aagaactagt tgccaaattg aatacgaagc cagtgatcat
     2461 ccacgcaagt aagggattag aacagggaac gcataaaaga atctctgaaa tcttgttaga
     2521 ggaaatcccg acagaaaaaa gaaaagctgt tgctgttctt tctgggccaa gccatgcgga
     2581 agaagtagca aagcatgaca ttacgacgat cacagcggca agcacagatg caaaagcagc
     2641 aaaatatgta caagagctgt tcatgaacca ttattttcga atctatacta accctgatgt
     2701 ggttggtgta gagatggggg cagctttgaa aaatattatt gccattggag ctggtgctat
     2761 tcacggctta gggtttggcg ataatgcaaa agcggcaatc atgacgcgcg gattagctga
     2821 gatcagccgt ttaggtgtag caatgggtgc caacccgctg acctttattg gcttaagcgg
     2881 agtaggcgat ctagtcgtta cttgcacaag tgttcattcc agaaactggc gcgcaggcaa
     2941 cttgttagga caaggacagc ctctagaaga ggtgctagcc aatatgggaa tggtcgttga
     3001 aggtgtgaat actactcaag cagcgatgga actagcccaa atgatggaaa tcgaaatgcc
     3061 catcactcaa acaatctatg aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa
     3121 agaaatcatg ttacgtgatg ggaaaaaaga aaatgaattc tcattaacaa ttttttgaag
     3181 gagcgaatgt atatgaaagt aaaaaaagca gttattccag cagcaggatt aggaacaaga
     3241 tttttaccag caacaaaggc gatggcaaag gaaatgttgc caatcgtgga caaaccaacg
     3301 atccaattca tcgttgaaga agcgttgaaa tcaggaatcg aagacatctt gatcgttaca
     3361 ggaaaagcta aacgaccaat cgaagatcac tttgattcaa atatcgaatt agaaacaaac
     3421 ctaagtgaaa aaggaaaaat cgaattattg aaattagtag aagaaacgac agatgtcaac
     3481 ttgcatttca tccgtcaatc tcatccaaag gggttaggac acgccgttct tcaagcaaga
     3541 gcctttattg gaaatgaacc gtttgtcgtt atgctaggcg atgatatcat ggaagacgaa
     3601 gtaccattga cacagcaatt gatcgatgac tatgaacgta cacacgcttc tacgattgcc
     3661 gttatgcaag ttcctcacga tgaaacttca aaatacggaa ttatcaaccc aggggaagta
     3721 ctggaagatg ggttatacaa tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca
     3781 ccaagtgatt tagctattat tgggcgttac ttattgactc cagaaatctt tgatgtatta
     3841 tcagaacaac aaccaggtgc tggaaatgag atccaactaa ctgacgcaat cgatacgttg
     3901 aataaaacac aacgcgtatt tgcccgtaaa ttcacaggta aacgttatga cgttggtgac
     3961 aaatttggtt ttatgaaaac aagtattgaa tatggtctga cacatccaga agtaggggca
     4021 cctttgcgtg aatacatcat gtctttaggt aaaacattag aagcagaagg aagcaagatc
     4081 gatcataatc caaaaggaat aacagaaatc aaagaagaaa agaaaaaata atcatttttt
     4141 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     4201 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     4261 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     4321 tcattcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     4381 aagcaaatac gacaattgag atcgtcacaa aagacgtaga tcttttatct agacaagtag
     4441 aaggcttgct ggtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     4501 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     4561 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     4621 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     4681 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     4741 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     4801 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     4861 ggcaaaagaa aaatcagctg agttaacagg cattgcaaaa gaaaaagcgc aagatctttc
     4921 tcaacaagca ggggatctag caggtaatgt taagcaaaag gtaagtgaat cagcagagga
     4981 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     5041 taatcgtttc aaaaaaacag cagaagatct gaaaaaacca gcagacgaat taggtagtat
     5101 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     5161 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     5221 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     5281 agattcgact accaatcact aagaatactc atttctagaa taaaaaggtg agagcggacc
     5341 agctcccacc ttttttattt tcattactct aaaacaagta attcttttgg tgtagtcgtg
     5401 aatggcacac aaccatcact tgtcacatac acacaatctt cgatacgtac ccctactttt
     5461 tcaggaatat agattcctgg ttcaagagag aagcacatcc cttcttcgat cactaagtcg
     5521 tttccttcta caagagaagg gaattcgtgg acggttgtcc caattccgtg acctaaacgg
     5581 tgattgaaat attcgccgta gccatagctg ttgattacgt ttcgtgcgat ttgatcaagt
     5641 tcacctgctg tcacacctgg gcgaactgcc tcttgtgcgg caagctgtgc ttcaagagtg
     5701 atgttgtaga ttttttcttg gaattctgtt ggtttttgat aagcaactgt acgagtagca
     5761 tcactgcaat agccattcca aacaacgcca agatcgaata acactagttc atttggtgaa
     5821 accttcgtgc ttcctggtgt accatgaggg cttgcagcat ttgctcctgc aaggactaag
     5881 gtgtcgaaag acatttgtga aacaccttgt ttttttaatt ggtattcgat ttctgcgata
     5941 attgcttgtt cagtcacgcc ttcttttact gttttgaagc caatttcaaa ggcaacatcg
     6001 gcccatttac cagcttcgat cagtttgtct ttttcttcta ccgtcttgat cagttgcaga
     6061 cgctggataa tagaagttag atcagtgtaa gaatctgcac ctgtcaagat atcagagata
     6121 gcatcgaagc gatcgacagt cagagcattc ttttcaatcc ctacattacc aggttgccca
     6181 tagcgtgcgc gaagctcttt tccgatgatc tcccaagggt tttcgctgtc taagtagcca
     6241 cgtacatcgt acgcccagct gctgttttga gcatcttcaa cttccagagc aggagtgaaa
     6301 aggaaaggat cttgttcaag agggataaaa agagctagta cacgctcgtg aggatcactt
     6361 tcatatccgg aaaagtaagc gatgtggcca gggtcgctga tataagccaa ttctacttgg
     6421 tgttgtgcca tccattgacg tagttcattg atttttacgt tattcatttg gcaacctact
     6481 ttctaaatta attttgtatt caataattgt accactgatt gattaaaaga ccaacgttct
     6541 taaaactgaa aaaagtaaaa attttcatga aaacagagat aaaacggttg caattgttga
     6601 gattatttga tatcctattt tagaaatgta agacgtattt tcagaaaaca tataaaatat
     6661 gaataggaga acacaaatgg aaaagcaaac aatcactatc tatgatgtag cgagagaggc
     6721 aaatgtttcg atggctactg tttcacgtgt cgtaaatggc aatccaaacg tcaaaccagc
     6781 aacaagaaaa aaagtcttgg aagttatcga tcgcttggat tatcgtccaa atgcagtcgc
     6841 acgtggcttg gcaagtaaga aaacaacaac tgttggggta atcattcctg atgtcagcaa
     6901 tatgttcttt gcctctttag cacgtggtat cgacgatgta gcaacaatgt acaaatacaa
     6961 tatcatctta gcaaactcag atggcaacga tcaaaaagaa gtcaatgtat tgaataattt
     7021 attggctaag caagtggacg gcgtgatctt tatgggtcac catatcacag acgaaatccg
     7081 tggagaattt tcacgttcaa aaacacctgt tgtactagct ggttccattg atccagatga
     7141 acaagtaggt agcgtaaaca ttgattatgt atcagcaaca aaagatgcag taaataaatt
     7201 agcaaaaagc ggcaataaaa aaattgcttt tgtcagcggt gcgctgattg atcctatcaa
     7261 tggtcaaaac cgtctaaaag gctataaaga agcattaagc gaaaacaatc taccatatag
     7321 cgaaggatta atctttgagg cacaatacaa ctttaaagat ggtctgtctc tagttgaccg
     7381 gatccataat agcggtgcaa cagctgtcta tgtttcagac gatgaattag ccattggtat
     7441 cttggatggt ttattagacc gtggcgtaaa agtaccagaa gaattcgaaa tcatcacaag
     7501 caacaattct ttactgacag aagtagctcg tcctcgtttg acaagtatca cacaaccatt
     7561 atacgattta ggagcggtat ccatgcgcct attgacgaaa atgatgaata aagaagaagt
     7621 cgaagaaaaa acgattatct tgccttatgg aatcgaagaa aaaggatcaa ctaaataata
     7681 aaagatcaat catttagagg ttgttccaaa agtggccaac ttctggaaca acgtttattc
     7741 gagaaaaggg gttatgacaa aattttgtca taaccccttt tttttgtgac tttaaaaaaa
     7801 gagaaaatgg cggaaacaaa ccttacgtcc atcgtatacg acacaatctg tgcgtagatg
     7861 ctacattttg ttcactacta tcaaaattac tttaaaatga aaacgtgata aaaaagaaag
     7921 tagaaaagta gaaaagtaac tctaaaaaat ccaaaaaaat ccaggctcga ttgcgtactt
     7981 atcttaaagg agaaacaatc catcatacat aacccgaaag gagacttcta tttgcctaag
     8041 aaaagacgaa agaccatagt catcattttg accattcttg ctataggaat cggaggaaaa
     8101 ttttacatgg acaaaagaga agcacaaaga cagaaagatt tactcgcagt agaaaaacaa
     8161 tccgtcaaag ttctgaaaaa tacctttgca gatatcaggg aagtgaaggt agaagaattt
     8221 aaaaaaaacc ctgttactgg atgttatggt gcacttgtga caatgacaaa taatgaaggg
     8281 aaatctgttt attttgatta ttttttttca aaacaaatga atgaaattag cagttatggt
     8341 gtagaaaatg aagaaataca aaacgaaggg gttactactc ctaaaataag agttatttat
     8401 tcta
//
LOCUS       DACEPD010000099         8381 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.103, whole genome shotgun sequence.
ACCESSION   DACEPD010000099 DACEPD010000000
VERSION     DACEPD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.103"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(138..1106)
                     /locus_tag="IW797_002353"
     CDS             complement(138..1106)
                     /locus_tag="IW797_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ1225113.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            1338..2171
                     /locus_tag="IW797_002354"
     CDS             1338..2171
                     /locus_tag="IW797_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ1225114.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            2205..3044
                     /locus_tag="IW797_002355"
     CDS             2205..3044
                     /locus_tag="IW797_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1225115.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            3070..3630
                     /locus_tag="IW797_002356"
     CDS             3070..3630
                     /locus_tag="IW797_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ1225116.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            3829..5550
                     /locus_tag="IW797_002357"
     CDS             3829..5550
                     /locus_tag="IW797_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ1225117.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            5715..6857
                     /locus_tag="IW797_002358"
     CDS             5715..6857
                     /locus_tag="IW797_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ1225118.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            6873..8084
                     /gene="thiI"
                     /locus_tag="IW797_002359"
     CDS             6873..8084
                     /gene="thiI"
                     /locus_tag="IW797_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ1225119.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         2885 a         1456 c         1731 g         2309 t
ORIGIN      
        1 aaatagcttc ttcgaaaact aagtcgaaaa tttacaaaaa tttgagaaac aattttcgta
       61 aatttcttct tatttcttga agttgccccc ttctgtcaca gcctctcttt ttaagtttag
      121 ctattagata atgatgttta gttttggata aacgaaatca attcttcacc cgacaaacca
      181 tagtttacag caatcaatag ttttaaacgt gctttttgac tattaatact gttacagaaa
      241 ataatcccca ttttttctag ttcttttccc ccgccttcat aatcataaac aggctctgcg
      301 attccattga aacaacgaga aacaagtact acagggattt tttggacaag aagcttttcc
      361 aaagcagcta aggcttgtgg agggagattc cccgcaccta aagcttcgat taccaaaccg
      421 tctactttcg tatgggcgat ggcttccaat aagtcccctt gcatcccagc atatgcttta
      481 acgatcggga tagtcccatc tacagcggaa atgtccagtc gctttgtttc taaaagttcc
      541 tgcaagaata aaattcgatt cttagttact aagccgatgg gacccaatgt aggtgttcgg
      601 aaagtagcaa catttgtggt atgggtcttt gtcacgtagc gtgcagaatg gatctcatcg
      661 ttcatgacca ccaaaacccc cttatctagc gcttcttcac aacttgcgac acgaatcgca
      721 ctttcaaaat tataaaggcc gtcacttcca agttcattac ttgaacgcat tgccccagtc
      781 aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa aaaaagcagt ttcttctaat
      841 gtatctgtac cgtgtgtgat caccactccg gatatacctg atgcataagc ggacaagata
      901 cgttctttta ataccaacat gtgagaaggc atgatgtgtg gtgagggaag attgaagata
      961 tcttcaacta ataattctac atgcttgggg attgtgattt ctgcatctaa taatgggttt
     1021 gccacatcag gactaacacc gcccgtgagc atgttttctt tcatggcgat cgttccgccg
     1081 gtgtgtagta caagtatttt tttcatttta cagacttcct tacgaataat tttgcatagc
     1141 cgttaaatct tattgtatac tatataaaag agttttgcta tcagtgaaga ataacgaata
     1201 gcctaactta tcataaaaaa gctgacctta ttaaaatgga aaagactcaa ccttgacctc
     1261 ctaatacaat ggagtatctg gttgagtttc ttttacaaga aagcagccaa ggaaatcaat
     1321 cacgaaggtg gccggaaatg atcagaatcg gattaacatc gtttaaagaa catgacaaat
     1381 taactggtaa aaagaataac actctttatg aatatgcagg gtttctaccc ttagtggaat
     1441 tagatactgc ctattatgga attccccgaa agtcctcagt tgagaattgg ataagtgaag
     1501 tacctgaatc atttcgcttt gtagtcaaag tctatcaagg aatcagcggt caaggagact
     1561 ggaagcctta ttatcagacc gaagaagaga tgattgcgca ttttttagat gttatgtcgc
     1621 caatggtaga tgctggaaag atgttttgct ttttagtcca attcccctca tcattcaaat
     1681 gtacgaaaga aaatgtccag tacttgcgca aacttagaaa gtggtttgag ggttatcctg
     1741 tttctataga attgcgggat tattcttggt acagcgatga atttcttgaa cgtacaagaa
     1801 actttatgtc tagagccgct ttttctttag ccatcatcga tgagccacag ttgctgaata
     1861 caacgattcc ttttgatcca tttgtcacca atccgaactt tgctcttttt cgattccatg
     1921 gtaggaatgc gcaaggatgg caagcaaatg ataaagagtg gcgtaaaaaa agaacgttat
     1981 atcgttattc agaaaaagaa ctgcaagaac tagcacatga agtcaaagta gttgattcta
     2041 agacaaaaga ggtaggtgtg atttttaata ataattctgg tggtgatgcg gctgaaaatg
     2101 cattagcact gcaaaaaata ttaaatatca agccagatgg actcaatcca acccaaatgg
     2161 atctgtttta agggcttatt ttattcaaga aaggggcgaa gtctatgatc aaagctattt
     2221 tttttgatat tgacggaaca ttggtcacca atcgttcaaa agcgttggaa tcaacaaaac
     2281 aagcaattga atatgctcgg aaaaacggca ttttatgcgg tgtagcaacg gggcgcagcc
     2341 cagtaaagat caaagaaatc attgatgaat tggaattaga tatgtatgtc gtgtataatg
     2401 gtcaactggt ttttactgca gatcgcacga ttatcgacca cccttttgaa caaaaagtat
     2461 tggaacatat tgttgaattt gcagatgaga accatcgaca aatcgttttt ggtgcgagaa
     2521 atcggttaga tggatcaacg acgatgctat taggacaatc gatttttatc aagcgactag
     2581 tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa aaaaatcctc caggttttta
     2641 gccctcaccg acaaaaggat cgttatgaaa cgttagagat attaaaagaa ccaatttatc
     2701 aatgtatatt gctcagtccg gagtcagaac agaaaaagtt agaacatcgc tttcccgagt
     2761 gtacgtttca gcgttcaaat acgtatactg tagatatcat cccaaaaggt ggttcaaaat
     2821 tactaggcat ccaagctttc gcaaatgctg tcgaaattga aatggaagaa attatggcat
     2881 tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt aggaattgga gtggccatgg
     2941 ggaatgctca gatcgaagta aaacatgctg cagactttgt gactcaatca aatgaagaag
     3001 atgggatata cgaaggactg aaacacttcg gtctgattta ttagtcaatg aaagaaggaa
     3061 aaaatcagta tggaagcaga gacacctttt caaaaagtag aaacattcca caaaactttt
     3121 gatccaagaa gaccagagat acccacggct tttacagaaa atgaagctca tacacgtgct
     3181 gcgttcaaaa tagaagaact tgttgaattt gttgcagcta cttcacctaa taaagaacaa
     3241 ctgcatgaag ctgttatgct gctgcacgaa gcattagata aagcagaaga aaaaatactt
     3301 ggtaaaacac agtggggaag tgatccgctg gttgaacaag tagatgcact gacagatatc
     3361 ctttacttta cttatggttc tttttcatta ctaggaatag tgcctgatcg aatattcgaa
     3421 atcgtccatc aggcaaatat ggggaaatta tttccagatg gaaaacctca ttatgaccct
     3481 gtaacaaata aagtgttgaa acctgaaaac tggcagatgg aatatgctcc agaacctaaa
     3541 atcgcggcag aaatccaacg tcagattcaa gaagcgattg ctaagaagaa atccgctgat
     3601 ataggcatgt gcacaaatga aaaagagtga tcagagtttc actatttttg ttatctttat
     3661 acgatggaaa aacaaaagat tttcgtttaa gttagtgaat gcatgaaaat agtatgctat
     3721 aatctagaat agatgtcttt tgcacgagag acgtattatt tggatttatg ccaagcagaa
     3781 cgccatctgt tgcataaaag atgcatactg aaaacggagg aaccataaat gggaaataat
     3841 gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca ttgtcgtctt tttggttagt
     3901 tacttgatga agaaaaaaaa ccaagatcga ttggatgcct tagaaaaacg caaggaagaa
     3961 ttatttgact tgccagtgat tgaagaagtc gatgatgtta aaaaaatgca tctggttggg
     4021 caaagccaaa atacctttcg tgaatggagt caaaaatgga atgacatatc tacttcctcg
     4081 ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc tcaatgaaag ttttcggttc
     4141 ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa tggaccaaat ggagaaacaa
     4201 gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa cggaagaacg caactcacta
     4261 gctgttcagc aggcacttga cgtctatgaa gaactgaaag aaaaagtcag agaagacgag
     4321 catgaatacg gaccagctct ttttgaattg caaaaacaga tcaaagtgat cgaaaaagaa
     4381 tttacgcagt ttgtgacttt aaacacttct ggagaccctg ttgaagcccg cgaggttttg
     4441 gataaagcag aaaaacatac gtatgaagtg gaagctctga tgaaaaaaat tccaccgctg
     4501 tatgaggagc tccataccac tttccctgaa caacttgaag aaatcagtga tacttacgag
     4561 aaattgatgg aagaacaata tgtattccca gaagaagatc ttgctgaaga tatcgcaaaa
     4621 gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa aaacagaagt tgaaacggtc
     4681 gaatttgaaa accgtgaaac agctgatttg attgattcgt tatatgatat cttagaacgg
     4741 gaaatggaag ctcagagata tgtaaaaact aaccaatcaa cgattgcaga gtatatcaaa
     4801 catacaacaa agaataaccg tcaattattg attgagctgg atcacacatc tcaaagctat
     4861 acattgaacc acaatgaaat tggccgtgta cgaggtttcc aaaccgaagt ggaagaaatg
     4921 gaacgtcaaa atgaacagat gattccacag atccgtcaac acgaaattcc ttattcagaa
     4981 gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag atatcgaaaa gcaacaagta
     5041 gagatcgacg actcattaca tgaattgaga aaaggcgaaa aagaagcgca agaaaagatc
     5101 gatacattcg aattcaaatt gcgcagtttg aaacgttttg tagaaaaaca acgtttgcct
     5161 ggcttgccga atgattacct agaattcttt tttgttgcta ctgaccgtat tgaagaacta
     5221 agtgttgtct tgaataaaat tcgagtaaac atggaagagg tcaatcgttt agttgctcta
     5281 tgtgaagaag atctagaatt attagataag aagacacatg atttagtaga tgcagcagca
     5341 ttaacggagc aaatgctcca atatgcaaat cgttatcgtc atacacacga agaagtgaga
     5401 gaagcaattg ataaaagcta ctatttattc aataaagaat atcactatca agaagcatta
     5461 gacgaaatcg gtacagcatt agagcgagta gaacctggtg ctttcaaacg catcgaagat
     5521 ttttatttta atcatccaga cttagtataa agcaaaaaag ttagtaaacc agcaagagca
     5581 agacggatta ttgtcttgtt cttgtctttt tttgtatatt tgaaaattga aacaagtgaa
     5641 aatttcacct gctcttgaaa gcaccttgga caaccgttat aatagaaaag aatttcaaaa
     5701 aagaggggtt tcttatgata tattttgata acagtgcaac aaccccaatc tttccgcaaa
     5761 gcttagatgc atatgtaaaa accagtcagc ggattattgg gaatccatca agcttgcatg
     5821 atttaggcaa tcaagctaac cggttgttgc agcaagcaag aaagcagatc gctgatttga
     5881 tccatgtaga accagaagaa atctatttta cttcaggcgg aacagaagga gataactggg
     5941 tattaaaagg aacgatgatc gaaaaaagag aatatgggaa tcatatgatc atttcaggag
     6001 tcgaacatcc ggccgtcagc gagacagcag aacaattaaa agaattagga atcgaagtaa
     6061 gtatcgcccc agtggataaa cgcggatttg tacgtgtcga cgaattgaaa gaattaataa
     6121 gaaaagagac agtattagtc tctgttatgg ctgtgaataa tgaagtagga tcgattcagc
     6181 ccatccagga aatcagtgat cttttggatg attttcctaa aattcatttc catgtagatg
     6241 ctgttcaggc gattggaaaa gtaccattct cgcaatggtt gacagaaaga gttgattttg
     6301 caacattttc agctcataaa ttccatggcc cacggggaac tggtttcatt tattggaaaa
     6361 agggtcgtcg tttagcacca ttactgacag gtggagggca agaaagaaat cagcgaagtg
     6421 ggacagaaaa tgttccaggt attgtggcta tggttaaagc tttacgttta tcattagaga
     6481 taaaggaaca aaaacctgag cagacacaac gcttaaaagt agctctaatc aatgcgttga
     6541 agacgtatga gaaagtgacg attttttcag aaggaactga ctatgcaccg catattttat
     6601 gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca tgccttagaa gaaaaacaaa
     6661 tatttgtttc tactactagt gcctgctcct cacgtaaaca tatggcaagc agtacattgc
     6721 acgcaatgca tgtaccaaat gatctggcta catctgctgt tcgaatcagc ttagatgaaa
     6781 acaattcgct tgcagaagtc gagcaattta tgattatttt caagcagctt tataagaaat
     6841 tttctgcaat caattaaaag aggagtgttc ttgtgaaata cagtgaaatt atggtccgtt
     6901 atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt tatcatgcaa ctagcacaaa
     6961 atgtcagaca agctttgaaa gactttccag aaatcaaaat ccatgctgat cgggaccgga
     7021 tgcatctttt attgaatggc gcagacgggc agctagttat tccaaaacta gcaaaaatat
     7081 ttgggatcca aaatttctct ccaagtattc gtgtagaaaa agatgtcgaa gttttgaaac
     7141 aagctgttca agacatcatg aaagaaattt atactgggaa agaaacattt aaaattatcg
     7201 ctaaaagaag cgatcaccaa tttgaattga caagtaatga attgaatcaa acattaggta
     7261 atgctgtttt tgatattttc ccgcatatcc aagttcaaat gcgtcaacca gatattccat
     7321 tacgtgtaga gattcggcgt gatggcgctt atctatcgta tgaaacgatc aagggagctg
     7381 gaggattgcc cgtaggaaca agcggaaaag gaatgcttat gctttctggg gggattgatt
     7441 ctcctgtagc agggtacttt gccatgaagc ggggagtgga aattgaagct gttcattttg
     7501 ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc aaaagatcta acagccaaac
     7561 tagcacctta tgtgggaacg atccatttta tcgaagtgcc atttaccgag attcaagaag
     7621 aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat tactcgtcga atgatgttgc
     7681 gtttgaccga tgaaattcgc cgtattcgtc atggtttagt cattttgaat ggtgaatcat
     7741 taggtcaagt ggcttcacaa actttgcaaa gtatggtggc tatcaatgaa gtaacgaata
     7801 cgccaatcat tcggcctgta gcgacaatgg ataaacttga aatcattgaa gtagcagaac
     7861 agatcgatac atttaatctt tctatccagc cttttgaaga ttgctgtacg atttttgctc
     7921 ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca tcaatatgaa gagcgtttag
     7981 caatagatga aatgatccaa cgagcactag ctggcttgaa gatggaagag atacaagcag
     8041 aatcgtctaa tgaaaaaact gaagaatttt cagaattgct ataagaggtt atgaaaatag
     8101 tgttttgacc agagtattag agaaacaaaa cgaaaaaata gctccatgta ttttaccgtt
     8161 tttgaactaa tgtcgaagat tagctgattg aataccattt atcaagaagt tatggaaaag
     8221 ccggttcttt tttactaact gaaaaataaa attttattta gagatcaagc tacagttgat
     8281 aaatgatata aaaaagagaa tttcccatga aatagcgagg ttgtgacaga agtgggcagc
     8341 ttcaagaaat aagaaggaat ttacgaaaat tgcttttcaa a
//
LOCUS       DACEPD010000100         8274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.104, whole genome shotgun sequence.
ACCESSION   DACEPD010000100 DACEPD010000000
VERSION     DACEPD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.104"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(46..1821)
                     /locus_tag="IW797_002360"
     CDS             complement(46..1821)
                     /locus_tag="IW797_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ1225120.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSRRKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(2032..2934)
                     /locus_tag="IW797_002361"
     CDS             complement(2032..2934)
                     /locus_tag="IW797_002361"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ1225121.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEGGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQAFDPEVFPELLAEEVTL"
     gene            3213..5558
                     /locus_tag="IW797_002362"
     CDS             3213..5558
                     /locus_tag="IW797_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ1225122.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKVIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            complement(5721..5987)
                     /locus_tag="IW797_002363"
     CDS             complement(5721..5987)
                     /locus_tag="IW797_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225123.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNHQ
                     MTAEEAAQEFSNRHVHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(5980..6303)
                     /locus_tag="IW797_002364"
     CDS             complement(5980..6303)
                     /locus_tag="IW797_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225124.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            complement(6315..6521)
                     /locus_tag="IW797_002365"
     CDS             complement(6315..6521)
                     /locus_tag="IW797_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225125.1"
                     /translation="MTEQEIIEALASVVATKENLVDSAKEVYLLRINKARRMGEAFDT
                     LVKEIQDKINEIVTRDRELAQQFN"
     gene            complement(6518..7573)
                     /locus_tag="IW797_002366"
     CDS             complement(6518..7573)
                     /locus_tag="IW797_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ1225126.1"
                     /translation="MMADKQYSVISEAVYWLDPKHRDYDHTYKENSIKKIDSYNFKIL
                     KIKDSLDGMQAMGVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRPG
                     ASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALYV
                     AAEKQWKNVGLNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEVR
                     ISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQEL
                     NNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELEK
                     KWHGKKACGKHVLSPLR"
     gene            complement(7573..7968)
                     /locus_tag="IW797_002367"
     CDS             complement(7573..7968)
                     /locus_tag="IW797_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225127.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGAYRMFVTMINNHDESVYFTFGFWKERNELGDY
                     GVENKSIQKEGITVNKIRVIFSNNKEEYI"
     gene            complement(8011..8265)
                     /locus_tag="IW797_002368"
     CDS             complement(8011..8265)
                     /locus_tag="IW797_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225128.1"
                     /translation="MTVKDEEYRYISEHVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKDSLDGMQAMAAAPIVHSKLEKNFKNKKIPTNFRVLK"
BASE COUNT         2432 a         1680 c         1495 g         2667 t
ORIGIN      
        1 ttcggaaaaa ggaactgcga caagactttt gtcacagttc cctttttatt tagaagataa
       61 agcttttttc agattcaatt tactgaaaac tagcatgctt gttactacga tgaattgtgt
      121 taatggcacg cctgcgccag tgtagacact taaaaaataa tccttttttt ctggcgcata
      181 agaacaaaac cacgagacac cgaaaagaaa aaccaattgt gtcagtatta atccggtgac
      241 tacaatcaaa gaggaactat aataggcacg atcagctttt gcaaatacgg ttcctagtat
      301 cataagtaat atccctgcta ttgaagcaat cagcacggga atggccagaa cttggtaaat
      361 ttttatcatg acattcgcga cttttgctgt ttttgtcaac tggaaatcag gtgttctcgg
      421 atcactgcta tcgatccaat tatccattag tggaagattc aataattctt gggcctgttc
      481 gacttgttct tgcgtcccga aactataatt ccctccttga tggaaatctt tgtatcgcca
      541 tgcataggca tatcctgatt tcagaaaagc aaatacgatt ggcagatcat tcatcgtctt
      601 tccatcccct gatcccatca gatagaattc ttttttcttt ttgatagttc ccttttgata
      661 ggcttcttct aattcttgat tgactttttt ccaaaaatca ttggtttctt ttgcattgtt
      721 ttggtaataa ccagattcgc ttaccacatc tcgcaatgcc caaaaaatga tatctcctgg
      781 taattctttt acttttccgc cttgggtcca cgcatgagtc gtatacaatt cgtcaatacg
      841 agtagaaaca gtatgaaatg taggagatgc gtctaatgct ttatttaatg cagcatgcga
      901 gacccatatc tcactgtttt ctttatttaa gttcgtttcg tcatctattc ggatcagctg
      961 tttcatcaaa cgtccaaatt caccactcga acggtcattg atggtggcaa taccatatgt
     1021 tgtctcgttt ttatattcga tccactgata gacgatggag aaaccgacta gagggagtaa
     1081 aaacaatgaa agtttcagcc attcttgttt cgaaaggaga aatggtcgtt cttttaactt
     1141 ctttgcttct ttgaaagaaa agtcttgtcc tagcaagatc tgaacgaaac tgatgcctaa
     1201 cgcgactaca gtaaaaggaa gaagccagat ggaatcttcg cgaatgaacc aaaagaaagg
     1261 aaaagaaaca gctaacccta aaaaccacgg cagcaatttt ctgcgggaac tatatttacg
     1321 gtaataaata gctagtaaac aagcgataat gatctcaaca gccgggatca cgagagcatt
     1381 acgatacgtt cgagtggaat actcatgtgt aaaactaatc ggagaaaaca aaaaataaaa
     1441 atagatactt aaaagaagat acttgttctt cacgatcggt ttaaacgcta gtgcaaccaa
     1501 tatgctggca agaatactga acatgccgaa caaaactgca taagaaagat gcagtttatg
     1561 tgcaagtact aaaaacaagc tgtaggagat cccttttgaa agtgtcgtag tcgtgtactc
     1621 ccctagccat ttaccatcca aaatattttg agcatagtga atcagcagct gatcatcata
     1681 ggttgccgac atatctgttc catagattgc tttactatat aaatacaacc gaataattgt
     1741 aaataatacg gctatgccaa gaataaagta tgtatgtttc ttttttagtt ggttgatcaa
     1801 gggttttaaa gtgttcagca tcttttcgtc ctttcaatca cgttttgcta cttgcttgtg
     1861 aatcttttcg ttttttactt ttctatcagt ataccctgtt tgtctgaatt taaaaagagg
     1921 gggcgaaagg agcatggcga aaacgactgc aaaaagcact cctttgtttc ttaaacaaaa
     1981 aaggacaagg cagactgccc aatccttttt tgttgctgtc agatagaaaa cttaaagtgt
     2041 gacttcttct gctaataact caggaaacac ttcgggatcg aatgcttgcg ctttgaacat
     2101 ggcaatttct tttccatatg gtgcgtaagc tttcttttta tctttaccga catatggagt
     2161 ttctaagatt ttcgaaatgt tgtcaaacat tggatcatgg acgattttgt tcaatgtgtc
     2221 aaatccaatt gttccaaaac cgatattggc atgccgatct ttatgagaac cttgtggatt
     2281 tttagaatcg ttgatatgga gtactttaag ccgatctaat ccgatgacct tatcaaactc
     2341 ttccatcaca cctgagaaat cttcttttat attatatccg gcatcattcg tatgacatgt
     2401 atcaaaagta acggataatt tttcattcaa ttttactcca tcgatgattt gcgctagttc
     2461 ctcaaatgtt cgaccgattt ccgtaccttt ccctgccatc gtctccaaag cgatctgtgc
     2521 cgtctgttct tttgtcaaaa cttcattcaa accctcaata attctagcaa tcccaccttc
     2581 tgcccctgct cctacatgag cgcctgggtg aagggtgatt tgagtagcac ctaaagcttc
     2641 cgcacgaaca atctcttctc tcagaaattg tacagcaaat ggaaacattt ctggtttgat
     2701 cgtatttccc aaattaatga tatagggcgc atgaacgacg atatttgtta gtccgttttc
     2761 tgccataaat gcttttccgg catcaatatt catctcttcg atagcttttc ttctagtatt
     2821 ttgaggagcg cctgtataga tcataaaggt cgaagatccg taactggctg cttcttctgc
     2881 cgcgcctaac agcattttct ttccgctcat gctgacgtgt gagcctaata acatggatgg
     2941 attcctcctt gaaaggcaat tgtttcgaac aattcctata tattggtaag atgatgtaca
     3001 aagaaaatgg tttgtttttc tttgccctgt tctccatagt aactgaaact ttctctaaag
     3061 aaaagcttaa gaactctttc ataaaaaaga tcatttcctc acgttttatc tcattcgaaa
     3121 aagaaattcc tgtcaaaaac agccttgtct gtttttttgt cttattattt gctataatta
     3181 gcgaaataaa accattagtt gaggtgaccc ttttgccacg caaaaatact cgaaaaaaac
     3241 agaatagaag aaaaaaagaa aaatggtttg ttcctaaggt tatttttaga gtgtttcaat
     3301 cattgactgt ttttattact gttcttcttt tgttgttcgc agctctaggt atcggaatag
     3361 gtgctggcta ctttgcctat cttgtcgaag acacacaatt gccgactaaa aaagaactgc
     3421 agacagaact aggaaacatt acggaaacgt caaagattgt ttatgcagat aatacagaga
     3481 tatcaaaaat ccagacagat ctgatgcgga cgactatttc atctgataag atttcgcctt
     3541 tattgaaaac cgcgattatc agtacagaag atgaatactt cgataaacac caaggttatg
     3601 tccctaaagc tgttttacga gcattagttt cagaagctac tggcatcggc tcgtctggag
     3661 gatccacttt gacacagcaa ttagtcaaac agcagatcct aaccgatgaa acaacattca
     3721 aacgtaaagc aaacgagatc gttctttctg cacaagtaga aaaatacttt tcaaaagatg
     3781 agatcattgc tacgtatttg aatgtctcgc catttggtcg gaataataag gggcaaaata
     3841 ttgctggagt ccaggaagct gcaagaggaa tctttggtgt agatgcaaaa gatgtgactc
     3901 tgccacaagc tgcttatatc gctggtttgc ctcaaagccc tatcacgtat agtccctaca
     3961 cgaatacagg cgcgctaaaa gaagatcttt ctgccggtct tgccagaaaa gattttgttt
     4021 tattcagtat gtaccgtgaa ggacaaatta cgaaagaaca atacgaagaa gcgaaagcct
     4081 atgatcttac aaaagacttt ctcccacaac aaatcgcgga acagactgac agagaattct
     4141 tgtattatac cgtgatgaac gaagcaacaa ggattattgc acaacaattg gcagaaaaag
     4201 ataatgctga tatgtcagat tcatcggtca gtgaagctta ttatcaaaaa gcacagcaaa
     4261 cgatccaaaa caaaggatat acgatccatt cgacgatcga taaagatatc tatgctgcat
     4321 tgcaaaccgg cgtagaaaat tacggctata tgttagatga tggtgctggt agccaagtgg
     4381 agactggaaa tgtattgatg gacaatagga ctggacgtat ctatggattc gtgggtggcc
     4441 ggaattacag ccagaatcaa aataaccacg cctttgatac agaacgtcaa gctggttcat
     4501 ccatcaaacc ggttcttgtt tacggtcctg caatcgatat gggacttgtc ggatcagaat
     4561 cccgtgtatc agattatgca acgacttggc aagaaggaga aaatgctggc gagaaaatcg
     4621 tcaatgcgac caatgaaggg tcgaacacct tccaaaccgt ccgtgagtca ctggaatggt
     4681 ccaacaatat cccagcttat cacttatacc aagacgtatt gaattcgggt ggatcgaaac
     4741 aatatgctta cgagcactac ttggcgaaga tgaactaccc agcaaatgat aattggggcg
     4801 ttgaatctgc tcctttaggg acagtagatg taacgacgct tcaacagaca aatggattcc
     4861 aggccttagc taatggtggc gtctacgaag aaggctatat cattgattcc atcacagata
     4921 atgcaggtaa tgtgatttat aaacatgaaa gtaattctgt ccgtatttat tccgaagcaa
     4981 cggcctcgat catgaatgac atgatgcgca gtgtgatcaa tgaaaaaatc actacaccat
     5041 tcaaagatgt catttcaagc ttaaacggaa atctaggaaa agcagattgg gtcggaaaaa
     5101 ctggttctac caacgaatac agagattcat ggctgatcgt ctctactccg tctatcacta
     5161 tcagtagctg ggcaggacac gatgacaata ctggaatgga cagccaagca aggatacgtt
     5221 cggctaatta tcttgccaat ctaatcaatc aagcttacca agcaaaccct gagattttcg
     5281 gcactgatga gaaatttgac ttatcatctg atgtgatgaa gaaaaaagta tctgggttca
     5341 caggacaaac agctggaaaa gttactgtca ataaaaaatc tatccaaacg ccaggaaaaa
     5401 cagtggaatc actttgggca aaaaatggtc cgaagaaaag cgcctttaaa ttcggcgttg
     5461 gcggtactga tgaaaactat acagattact ggaacaccgc ttcagcttat gctagagcga
     5521 atccagagac caaagaatca gacgataaag atgattgatc taatagatta atcagaatta
     5581 aagggagctg cgacaaaaat cttgtcacag ctcccttttt ccgaataaat ggtgttcaaa
     5641 agcttgcatc tacgatatta agacaaattt atgtaaaaat gtgaaaagcc attttcacaa
     5701 aatttttcta tataacttta ctaacgaaac tgttttaatt tcttttcttg tctttggaat
     5761 aattcggtta gtttttctgc aaattgccct tttactgatt ctttcaagtg tacatgtcgg
     5821 ttagaaaatt cttgagcagc ttcttctgca gtcatttggt ggttttgatc tgtcaatcgt
     5881 ttttctgccc aaatttgctg agatatcccc ataccaactg aacgtacatt tgatcgatgt
     5941 agatttttct ctaattcttt tagtaattgc attaaaaatt cattcataag atgatttctc
     6001 catagttatt tgtctgattt tttgacgata ggaacgttct agttcgtctt gctgctgata
     6061 gagttttcgt cgttctactt gtagggcttg ttgataatgc tcctgataat tcgaataact
     6121 tcgtcttgct tgaaacagag gctcattatc taaagctaag ttctttaata catgatgcag
     6181 tcgctgctcc tcttgctcca tcaatcgtaa tccttgattc ttaaagtgga tcaattcatc
     6241 ttcttgagct tctaattgtc ttcgttcact tcgataatct ctttccaatt cttctagttt
     6301 catctacttc tcctttaatt gaattgctgt gctaattctc gatctcgtgt cacgatttca
     6361 ttgattttgt cttggatctc cttcaccaat gtatcaaagg cctcccccat acgtcttgcc
     6421 ttattgattc gcagcagata gacttctttc gcagaatcga ctaagttctc ttttgtggcg
     6481 acaacactag ctaatgcttc tatgatttcc tgttcagtca acgaaggggc gacaacacgt
     6541 gcttgccgca agccttcttg ccatgccatt ttttctctag ctcaaaaatt cctctttgat
     6601 tgatgcggat aacttcttcc atagcgcttt tcatttttgc tgataacttt tccactacta
     6661 acctagcttg tgtgtcatct aagtaaattt gttcattaac tgtcagccgg ccgccactta
     6721 atcgtagttt tttacgtatg ttgttcagtt cttgcaactg ataggaaaac aactgggtcg
     6781 cttggttttc tttctgttgt tttcgagctt taacattatt ttcattttca ggaataatca
     6841 gctttccatt ttcatcaaac ttccaagagg ttaaactgtg ggaagcaagc agattcaaga
     6901 atgctgtttc catactgatc cgaacttcag cacctgtgcc attaccacca aaattaccga
     6961 taaaatcggc gtgatttcgg taattggtga gcataccggg attttcttcc acccattttt
     7021 ttgcttctgg tgataagaga tcatacgggt ccggcccgtt taagccgaca tttttccatt
     7081 gtttctctgc tgctacatat aatgctaaga atccacctaa tgaatggccg gtagtagaaa
     7141 tagcagcttc tccatattcc ttttttactt cattagcaaa tgcaatcgct gaattgattt
     7201 gccccgctgt tttcaatcca ctaacggata aaatcttgtt tgttgttgct ttttcagaaa
     7261 aacttgctcc tggtctattc ccatagagat tcatgatact ccttgcatcc gtttccaaat
     7321 ctttccaatc acttaagttc gttcctgcaa aagcaatcac tacttgagaa agatccactt
     7381 taccgttctt gattggcgct acacccatcg cctgcatgcc gtccaatgag tctttgattt
     7441 ttaggatttt aaaattataa ctatcaattt ttttaataga attttcttta taagtatgat
     7501 catagtctct gtgtttagga tctagccagt aaacagcctc actaataaca ctgtattgtt
     7561 tgtctgccat cattaaatat attcctcctt attattagaa aaaattactc tgattttatt
     7621 tactgtaatc ccctcttttt gtatactttt attttctacg ccataatctc ctaattcatt
     7681 tcgttctttc caaaatccaa acgtaaaata aacagactca tcgtgattat ttatcattgt
     7741 cacaaacata cgatatgctc ccgtcatttc atttcgagca gatttttcaa tcttcacttc
     7801 cttgatatcc gcaaaagtat ttttcaaaac tttgactgac tgtttttcta ctgcgagtaa
     7861 atctttctgt ctttgtgctt ctcttttgtc catatagact ttccctccga ttcctaatat
     7921 gacgattaat aaaatggcta agctgagttt tcttattttc cgagacaaga acgtgcctcc
     7981 tttcttttta tgtaagtatt gataccttca ttatttaagt acgcgaaagt ttgtaggaat
     8041 ttttttattt ttgaaatttt tctccaattt gctatggacg attggcgctg cagccatcgc
     8101 ctgcatgcca tccaatgagt ctttgatttt taggatttgg aattttgttt ctccaaagtt
     8161 tttaatggcg ccttctttgt aacttttact atattgagga tgttttggat ctagccaata
     8221 aacatgttca cttatatatc tatactcttc gtcttttact gtcacacttc ctcc
//
LOCUS       DACEPD010000101         8208 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.105, whole genome shotgun sequence.
ACCESSION   DACEPD010000101 DACEPD010000000
VERSION     DACEPD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.105"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            137..1330
                     /locus_tag="IW797_002369"
     CDS             137..1330
                     /locus_tag="IW797_002369"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ1225129.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1460..2932
                     /locus_tag="IW797_002370"
     CDS             1460..2932
                     /locus_tag="IW797_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ1225130.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3015..3803
                     /locus_tag="IW797_002371"
     CDS             3015..3803
                     /locus_tag="IW797_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ1225131.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3870..4538)
                     /locus_tag="IW797_002372"
     CDS             complement(3870..4538)
                     /locus_tag="IW797_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ1225132.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4653..6365
                     /locus_tag="IW797_002373"
     CDS             4653..6365
                     /locus_tag="IW797_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1225133.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6368..8140
                     /locus_tag="IW797_002374"
     CDS             6368..8140
                     /locus_tag="IW797_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1225134.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2513 a         1380 c         1807 g         2508 t
ORIGIN      
        1 ttttttaagc aaaaaaatga aaaaatacga gcagaaaact gttttatctg ctatacttgg
       61 ttcgttgcaa gttcccgaaa ggatccgcag gacggagatg ccttgtaacc gaatttaact
      121 agggggaatc attataatgg tagaaagaca cttatttaca tcagaatctg tttctgaagg
      181 acatccagat aaaattgctg accaaatcag tgatgcaatc ttggatgcaa ttttaaaaca
      241 agatccaaca gcacgagtag cgtgtgaaac gtctgtaaca actggtcttg ttttagtatt
      301 tggagaaata tctacgacgg catatgtaga tattcaaaaa gtcgtacggg aaacaataaa
      361 agaaattggt tatacacgtg caaaattcgg atttgatgga gatacagcag ctgtattggt
      421 tgcgattgat gaacaatctc ctgatattgc tcaaggagtt gatgaagcac ttgagatccg
      481 ggatgaagat aaaaaagacg tattagatga gattggtgct ggtgaccaag gtttaatgtt
      541 tgggtttgct gtagatgaaa caccagaatt gatgccttta ccaatcgctt tgagtcatcg
      601 tctggtacga cgcttggcag atttgcgtaa atcaaatgaa ttaacgtatt tacgtccaga
      661 tgcaaaatct caagtaacgg ttgaatatga tgatcaagga caaccggaac gcgtagatac
      721 aatcgttatt tcaacacagc atgatgatgc agtagataat gaaacaattc gtcatgatgt
      781 catcgaaaaa gtagtgaagg aagttattcc agctgaatta ttagatgatc aaacgaaata
      841 ttatatcaat ccgactggcc gatttgtcat tggtggtcct caaggggatg ccggattaac
      901 aggaagaaaa atcattgttg atacgtatgg cggttatgct cgtcatggtg gcggtgcttt
      961 ttctggtaaa gatgcgacaa aagttgaccg ttctgccagc tatgctgcac gttatattgc
     1021 taaaaacatt gttgcagcag ggcttgctcg caaagcagaa gtacagctag cttatgcgat
     1081 tggtgttgct caacctgttt cgatctcaat caatactttc ggtactggaa cggttccgga
     1141 agaagaactg attgctgcag tgagagaaaa cttcgatctt cggccagcag gaatcatcga
     1201 gatgcttgat ttgcgtcgtc cgatttacaa acaaacagct gcttacggtc attttggtcg
     1261 tacagatgta gatttgcctt gggaacatac ggacaaagta gatgcgttga aagcaagttt
     1321 agcaaaataa gttatattca gctggaacga tcgccaagtg gtggttgttc cagctttttt
     1381 tatggcagtt aacctcttga aaaagatagt aattatttat ctatttttag atcctgtcat
     1441 aatgaaaggg aggaataaga tgaaaaaaga aacaaatgtt actttggtca cagtgagtat
     1501 tttcattgca acgttcatga cggctattga aggtacaatc gtaacaaccg ctatgccaac
     1561 gattgtcggt tcattacatg gaatggaaat catgaactgg gtattttcga tttatttgct
     1621 gacaaacgct atgcttactc cgatttacgg aaaacttgcg gataaagtgg gacggaaacc
     1681 aatctttatt tttgggacat tattgtttat tatcggttcg ttattttgcg gtttatctca
     1741 aagcatgatt gggttgattg ctgctcgtgc gattcaaggg atgggtgctg gggcaatgat
     1801 gccagtagct ttgacgatca tcggcgatct ttattctatt gaaaagcgag caaaagtttt
     1861 aggtttgaat agttcagcct gggggattgc cagtgttttt ggaccgcttg cgggaggatt
     1921 catcgttgaa acgatcagct ggcactggat cttttttatc aatgtgccga tcggaattct
     1981 attaattatt ttgatttggc tatatttaaa tgaaccaaaa atcatacatg agtctaaacc
     2041 aatggatatt tttggtagtt ttagtttgat ggccatgctt gtctcattgc tgttagggtt
     2101 ccaattatta ggagacgaag gaatgagtcc gcttgtttct gctttgctgc taggtagtat
     2161 cgtaatgtta ggtatttttg tttttgccga aaaaagagct aaagatcctg tcatcatgat
     2221 gcatttattc aaaaatccac tatttgttgt ggtcaacttg gctgcagcat tggctagtgg
     2281 ttttttgatg ggaatcgatg tttatatccc tatgtggatg caaggtgtat tagggctgaa
     2341 tgcagcaatt ggtgggctgg tattagcacc attatcacta gtctggatgg tcggttcttt
     2401 ccttgcaagc aagtggatga ctcaaatgaa aactcattgg gtcttgagaa ttggactact
     2461 aatcgcagct attgggggcg cttggttgta tcttatgcct ttcagaagta actatttatg
     2521 gttttttgct atatcaacgg tcatcggtat aggcttaggt gtgaccatga ctacctcgac
     2581 tgtcagtgca caaaatagtg tatctcgtga ccagcttggt gtagcaactt cttttaatac
     2641 gttagttcga acaattggtc aaacagtcat ggtttctatt tttggtttat tgataaatgg
     2701 ggtaactaaa cgagaacttg cagcagcaaa tttaacaaat gatgcagata tcatgaataa
     2761 actggtgaat ccacaaacgg ctaaatatat tgatgcggat attttgttac cattaagaaa
     2821 aattctttat gatgggttac attccgttta tctagttggc ttgttactcg tgatcattgc
     2881 tttggtctta acgcttttcg ttcatgaaaa aagtggtaaa ataaaacagt agtttcggtt
     2941 aagatatgta cgtagccgaa tcattcaaat ttaaagttat tcaaaaataa cttcttacta
     3001 aggaaggcga aaatatggca tttattcaag caaatattta ttcgaatgtg ttagaaatgg
     3061 aagtcaatct aaatgtgata ctaccgcaaa caacaaaaaa ggtgattggc accagttcag
     3121 agcaagagac aacggatata cctgttttat atttacttca tggaatgggt ggcaatcata
     3181 gtgtatggga aagacgtacg tcgattgaac gttatgtagc aaactacgga ttagctgtca
     3241 tcatgccatc tactgatctt ggctggtaca cagatacagt ttatgacatg aagtattgga
     3301 cattcattgc agaagagcta cctgagattt gtcatgaatt attcccgcaa ttaacaagaa
     3361 aaagagaaaa aacatatgcc gctggattat cgatgggcgg atatggcgcg ttaaaactag
     3421 gccttgcaaa atcagaaaat ttcgcagcag taatctctct ttcaggcgct gtttctgtag
     3481 gaaatcgaat ggatgatctt ttgatggttc gtaaagcatc tttttgggaa gggatctttg
     3541 gaccattgga tcaaatcgaa ggttcaaaaa atgatccgca atacttattg aaagaactag
     3601 taaaaagcgg aaaaccagtt ccaagatttt atatggcttg tggggaatct gattttcttt
     3661 atcaggcaaa tcaggaaatg gtatctgcta tgcaggaaca aggcttggcg gtcacctttg
     3721 aacaaggacc tggcgatcat aactggatct tctgggacca atggattcaa cgtgcattgg
     3781 cgtggctaat ggaagaaaaa taaaaaaaga gtcttaaaaa gatagctcct atagagatca
     3841 caaacatagt gatctctata ggagcttttt tactgctcgt tttctcttct tttattttgt
     3901 tggcataagc cttcaatata ggtttgtgca ctcgttagaa agagtgcttt ttcattcaat
     3961 tctgcatggg actgcatact ttctttaaat ccctctaatt gattttcaac tacataatta
     4021 cgcataaata gaactgtttc tttcaaaaat tcatttccgt ccataatttt ttgtcgaagc
     4081 tgctgttcat gctgctgatc cattaactgt ccaatcaata aattgtggag agaggcaatg
     4141 gcctgatgac tttgggtgac tgaaaatcct tcacgaacaa gtaattggat catttgctcc
     4201 aaatgttcta atcgactcgg aaaagcgggc acagtattga tttctaattc tgccccacaa
     4261 gggaaacgtg tataaaggtc atagtaattt tccatatact gcagcaactg ttcattccaa
     4321 ggcaaagttg gatctggaag cgctaattct tcttccattg tttcagccat tttttgcaag
     4381 agctgttgtt tgtttttgaa ataccaatat aaagcaggtg cttgaacatg aattctgttt
     4441 gcgatgtttc gcatggaaag ctgcgaaaga gcttgtgttt cagccaggat atcaaaggcg
     4501 gcctgaatga tgattgcttg agaaagcttt atttccattt tatcaacctc gtgtattgca
     4561 ttatcttttt aaacatggta aattgttagt cggtctaatt ttacagtgtt aaattagtcc
     4621 tgtcaattta aacaaagggg tgtaatcaac gaatgaaatt aatcttacga catgcgaaaa
     4681 aatataaagt agctgttttt atttccttgc tgtccgtagc agtcatggtt gcagcagcat
     4741 tatggcaacc taagttacta cagcaagtat tggaagctat tattacagaa gataatgatg
     4801 aaatgaaaac aattggtatt tacctgatca gtctggcttt gctaggatta gccgcaggtg
     4861 taacgaacac gattttctct gcaaaagtag ctcaaggcgt aagtgcagat atccgagaag
     4921 aagcatttcg aaagatccag acattttcat ttggaaatat cgaacagttc tctgctggaa
     4981 atcttgttgt tcgtctaaca aatgatatca cgcaaatcca aaatttagtg atgatttctt
     5041 tgcaatcact ttttcgaatt ccattcttat ttattggtgc cttcatttta gctatgatca
     5101 cgatgccgca attatggtgg atcatcgtag cgcttatcgt tacggtattt ctgattacgg
     5161 cactttcatt tacaaggatg ggcaagcact tcatgatcat tcaaaaactg attgatcgtg
     5221 tgaatagcat tgcaaaagaa aacttactag ggattcgtgt agtgaaatct tttgtacaag
     5281 aagaaaacca attgaagtca ttcagcaaag taagtgagga cttaacaaaa cacaacatca
     5341 tagtcggtac attattttct gtgatgatcc cgtccttcat gctggctgcc aatttggctg
     5401 tggttggggc gatctttttt gtgagcgatc ttgttaaaga tgatcctacg ttaattggtg
     5461 gaattgcttc attcatgaac tacttgatgc agattatgat ggcaatcatt attggcggaa
     5521 tgatgatgat gatgacttca agagcagccg tatcgctgaa gcggattgcg gaaattttag
     5581 atgctgaacc agatcttact tatttagacg ttccagaaca agagttgacg ggttctgtca
     5641 cttttgacca tgtttctttt cgttatcctg gtgaggatac agatacgctt aaagatatca
     5701 gcttttctat caaacctgga gaaatggttg gtatcgtcgg ggcgactggt gccgggaagt
     5761 caacactagc acaattgatc cctcgtctat ttgatccgac agaaggcaaa gttgaagtag
     5821 gtggtgtaga tcttcgtcaa gtaaatgaaa agagtttaag acaaacggtt tcctttgttt
     5881 tgcaaaaagc aattttattc tcaggaacga ttgctcaaaa cttgcgccaa gggaaaaaga
     5941 atgcaacaga aaaagagatg gaacatgctt cttctattgc tcaagcaaaa gaattcatcg
     6001 aaaagttatc tgatcgttac gaagcaccaa tagaagaacg tagcagtaac ttttctggtg
     6061 gtcaaaagca acgtttatcc attactcgtg gcgttatcgg taatccaaag atcttgatcc
     6121 tagatgacag tacgagtgca ctggatgctc gctctgaaaa gctggtccga gaagcactcg
     6181 atcgagattt gaagggaaca acgacgattg tcatcgcaca aaaaattgct tctgttgtca
     6241 aagcagaccg catcttagta ttggatgaag gcagattagt cggcgaagga acccatgagg
     6301 aattggtagc tggaaaccgt gtctatcagg aaatctttga aactcaaaaa ggaacggagg
     6361 agtaagtatg actgatctaa taaaagcaag taaatttttc tatcactatt tgaagcggta
     6421 caaactctcc tttttcttta tctttatcac agtgatcatt gctacctatc tacaagtaaa
     6481 ggcacctcaa tacatcgggg aagcttttca agaattagct aattacgtag gaggcttgat
     6541 gcaaggcgta gacgataaaa gcaaatttgt ttctgtaata tggaaactct tgctgtttta
     6601 tgtactagct agtgcagcca actttatcta tagtattttg ttcacacaag tagtagggaa
     6661 gtcaacgaac cgaatgcgta tcggtctatt caacaaattg gaaaaattga cgatccgttt
     6721 ctttgattct catcaagacg gtgaaatttt aagtcgtttt accagtgact tagataatat
     6781 tcaaaatagt ttgaaccaag ctttgcttca agtcatcacg aatgctgttt tacttgtcgg
     6841 gatcttgatc atgatgttcc gccaaaatgt agagttagct tgggctacaa ttgcttccac
     6901 tccagtagcc gttttgatag ctgtcttgat tatccgcaaa gcacggaaat atgtggatct
     6961 tcagcaagat gaagtgggga aactaaatgg ttatatggat gaaaaaatca gcggacaacg
     7021 tgtcatcatc acaaatggct tacaagaaga aacgattgaa ggattcttag agcataatca
     7081 aagtgttcgc gaagcaacct tcaaaggaca agcttattct ggtctactat ttccgatgat
     7141 gcaaggaatg tcactggtca atacagcgat agttatcttt ttcggcggct ggttagcatt
     7201 aaatggtgat ttggaacgtt cggtggcatt aggcttagtt gtgacattcg tccagtattc
     7261 acaacaatat taccaaccac tgatgcagat ttcatctgga tacagcatga tccaattagc
     7321 gattacaggg gcaagacgat tgaatgaaat atttgatgaa aaagatgaag tcaaaccaga
     7381 aaacggtaaa gcatttgatg gtgttgaaaa aggtcttgcg ttgcagcacg tagattttgg
     7441 ttattctcca gaaaaactag tcttgaaaga tgtctcgatc aatgtggaca aaggagaaat
     7501 ggttgctctt gttggtccaa caggatcggg gaaaacgacg atcatgaatc tgctaaaccg
     7561 tttttacgat gtcaatgatg gttcagtagt catcgatgga atagatatcc gagattatga
     7621 tcttgatgca ttacgtagac atgtaggaat cgttttgcaa gaatctgtat tgttctccgg
     7681 aacgatcaaa gacaatatca cattcggcaa accggacgct tcagatgaag aagtcatcaa
     7741 tgcagcaaaa caagcgaata ttcatgactt catcatgacg ttagaaaaag gttatgatac
     7801 agagatatcc gaagaaaata acttattcag tacaggtcaa aagcagttga tcagtattgc
     7861 tcgaacgatc atcacaaatc catctttatt gattttggat gaagcaacaa gtaatgtaga
     7921 cacggtaaca gaagcaagga tccaaaaagc aatggatgaa gcaatcaaag gaagaaccag
     7981 ctttgtgatt gctcaccggc tgaaaacaat cttgaatgca gatagaatcg ttgtcttgcg
     8041 tgatggggaa gtcattgaag aaggcagtca tcatgagtta ttagcagaaa atggatttta
     8101 tgcggaactt tatcataatc aatttgtatt tgaaagttaa attaaaagaa cctcgttata
     8161 acggggttct ttttatatat tttatgttgt ttgtttttat aaggggca
//
LOCUS       DACEPD010000102         8061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.106, whole genome shotgun sequence.
ACCESSION   DACEPD010000102 DACEPD010000000
VERSION     DACEPD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.106"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IW797_002375"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="IW797_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ1225135.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="IW797_002376"
     CDS             complement(2944..3378)
                     /locus_tag="IW797_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225136.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..4600)
                     /locus_tag="IW797_002377"
     CDS             complement(3395..4600)
                     /locus_tag="IW797_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ1225137.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5294..5680
                     /locus_tag="IW797_002378"
     CDS             5294..5680
                     /locus_tag="IW797_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ1225138.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5774..5965
                     /locus_tag="IW797_002379"
     CDS             5774..5965
                     /locus_tag="IW797_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ1225139.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6479..6811
                     /locus_tag="IW797_002380"
     CDS             6479..6811
                     /locus_tag="IW797_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1225140.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7107..7262
                     /locus_tag="IW797_002381"
     CDS             7107..7262
                     /locus_tag="IW797_002381"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ1225141.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7538..8061
                     /locus_tag="IW797_002382"
                     /pseudo
     CDS             7538..8061
                     /locus_tag="IW797_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2664 a         1443 c         1186 g         2768 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtcctt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4441 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4501 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4561 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4621 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4681 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4741 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4801 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4861 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4921 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     4981 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5041 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5101 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5161 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5221 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5281 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5341 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5401 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5461 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5521 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5581 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5641 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5701 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5761 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5821 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5881 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     5941 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6001 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     6061 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     6121 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     6181 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     6241 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     6301 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     6361 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     6421 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     6481 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     6541 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     6601 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     6661 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     6721 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     6781 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     6841 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     6901 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     6961 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     7021 caactcgtat tctacgttac tatatggtag attcacacat gaaaaaagag attgtcgtcc
     7081 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     7141 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     7201 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     7261 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     7321 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     7381 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     7441 ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttaaaa tcctttagaa
     7501 taaagtcgac aaaaaccatt caaagtagtg tttcaagatg tcctattcag agtttaccaa
     7561 agagttacta gatattctag acctaaatct tacctttcat gaggatgcct ttcgcaaaga
     7621 gcgcatcaat gacgaaacat gttttgcttt tgatggcaca ttgacctatc aaccggagga
     7681 atgctttcat cgccactatc aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt
     7741 atccatctta ttgaatgatg tcagtaacta caagaccatt ctgcggatta acaaacagcg
     7801 cttcaaatgt aaacactgtg gaaagacttt tttagcagaa gattcagtta gtgatcgtcg
     7861 ctgctccatt gctcgcagag tcaaacaagc cattcttgag ctattaagcg aacctctttc
     7921 tatgtctttg atcgcacgta tgaagcacat ttctccgacc actgtcattc gaatacttcg
     7981 cagccttcgg cctaaaacca tttctttgag ccagctgtta ccagaagtgg tgtgtttcga
     8041 tgaattcaaa tcagtgaaaa a
//
LOCUS       DACEPD010000103         7910 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.108, whole genome shotgun sequence.
ACCESSION   DACEPD010000103 DACEPD010000000
VERSION     DACEPD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.108"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(25..573)
                     /gene="aac(6')"
                     /locus_tag="IW797_002383"
     CDS             complement(25..573)
                     /gene="aac(6')"
                     /locus_tag="IW797_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1225142.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1126..2229
                     /locus_tag="IW797_002384"
     CDS             1126..2229
                     /locus_tag="IW797_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1225143.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2422..3489)
                     /gene="rlmN"
                     /locus_tag="IW797_002385"
     CDS             complement(2422..3489)
                     /gene="rlmN"
                     /locus_tag="IW797_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1225144.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3719..6166)
                     /locus_tag="IW797_002386"
     CDS             complement(3719..6166)
                     /locus_tag="IW797_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1225145.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6355..7791)
                     /locus_tag="IW797_002387"
     CDS             complement(6355..7791)
                     /locus_tag="IW797_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1225146.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2190 a         1723 c         1352 g         2645 t
ORIGIN      
        1 tacttctgtc acaacctctt tttattattg agaatttggt cgaggaataa tcgtttttgc
       61 catccaaata tccggtttgt cccagccatt tgcatttggt aagacaccta cgattttata
      121 acctaatttt tcatagaatt catacggatg ttcacgaagg ttctggatgg aagccacttt
      181 atcaaatgta tgctcataca ggtccgtttg acttaacgtt gttccatggt ctaaatcatc
      241 cgtacctaaa taaatcgtga ttcctcctct ggaagctact tctttttcta agtaattgac
      301 taatcgagta cctatttggt tctttcgtcg ggagctttct acaactaatg gatgcaattc
      361 ccaacctgtg ataccgtatt gagggattgc accaataaat cctactaact catcttggtc
      421 taccgctgct accgcgattc gttctggatt catcatttct tctacttctt ctgccgagct
      481 gtctccatat tcttccggcc aagtcagtct cagtaaatca gaaagctgat ctttcaatac
      541 tggattatta cggtcaaatt cactgattat catacgtctt cccccttgtt cttgcttatt
      601 ctaacagtcc ttttgaaaaa acgaaatgtt ctcgctcaaa aatctttagc taccaatcag
      661 tatctctatt ctttcattat ttatcttact tataaaataa aaaaattata cagaaacact
      721 ttattcaaaa taggaaagat caggaaagcc atcaattgga ggaaaaaact ctgtttgttc
      781 atcatgatat agattatctt ttgctttaaa aaaaaccaat tatgaattga caaataaaac
      841 ctgttttcat ataataatct taatttttag aatttttaga taattacaaa aaagcaaagg
      901 gaatcggtag aaatactaag aggaacaaaa attcattatt gtcgttttca atgtttttgt
      961 acttgttcgt ttttaggata cttattgatg atttcaaggt gttcatgcca ccttattcat
     1021 cacttatcct gtcttaggtt cacctctact ccatctacag ccgcagtcag atcgatcact
     1081 ctttaaaatt ttaacccgtt tcaacgaaga aagaaggaaa tgaatatgag tcaaagtgtt
     1141 ttcttaccaa attataccat tggaacagat gcgtatgaaa aagcaaatgg aatcgtcaaa
     1201 gaatatggga caaaagtagt gctgattgga gggaagactg cactttcaaa ggcagaaaac
     1261 aaaataaaag tagctttgac acaggaaata gaggttgtcg ccagtctctg gtacgggggt
     1321 aattcaactt atggaaatat tgaacagttg atccagcatg aaagtgtcca aaaagcagac
     1381 gtcctgtttg cagtcggtgg tggccgtgct tgcgatactg taaaagtcat tggtgaaaaa
     1441 ctagctaaac cagtcttcac ctttcccact attggttcga actgttcgcc cgtcactgcg
     1501 gtatgtgtca tctataacga agaaggcgta atggacggac tcttctttcc ttcaaaacca
     1561 cctgtacaca ccttcattga tacagcaatt attgccgaag cacctgataa atacttatgg
     1621 gcaggtattg gcgatgcttt atccaaagaa gtcgaatcta ctttttctgc tcgtggcgat
     1681 gatctaggct acacggatag attaggaatc caaatcggaa aaatgtgcat tgatcgtctc
     1741 ttgacttatg gtacgcaagc attgaaagat agtgccgacg atcgtccctc atatgcaatt
     1801 gaacaagtcg tttcggatat cattgttact accggattgg tttctgtttt agttaacaat
     1861 aactataatt ctgctatggc acactcattc tattacggta cgactgttct agaaaaaatg
     1921 gagggatacc ttcacggagc atctgtcagt tatggtgtac tgaccttatt gatgatggat
     1981 cagcagttcg atttgtttga taaaatctat gtgtttatga aaaagaacca acttccagta
     2041 tcgttaaccg acatgaatct caatgtcacg accgatttag aagcaatctt agataaagcc
     2101 atggcaacaa atgatattaa acatgtgcct tatgaaatca cccgagaaaa atttaaacag
     2161 gccattcttg acgtagaaac ttatcatgct acacaagaaa gccagcaagg atataagaaa
     2221 atcagttaaa taattgctaa aacccccatc aagcaggctg tcatcaaatt gaggacattt
     2281 ctttgcttga tgagggtttt tcttgtcaaa aaaagcttta tagctttcgc tatagaatct
     2341 tttctcttat ttaagttgaa cagttcctta attaagaaaa ctgctccttt tatggtaatt
     2401 gaaaaaacga gtaggatacg attatttggc aactgttttt ttcatttgct tgcttcgcaa
     2461 ttgtccgcaa gctgcatcaa tatctgtacc atgttcttta cgtatcacac agtttacgcc
     2521 gtttttcttc aatacatcat aaaatctcaa tacatcagct tttggactgc gagaatattg
     2581 atcatgttca ctgactggat tatatggaat caagttgaca tatgctaatt ttttcttatc
     2641 ttttaataga tctgctaatt gttgcgcatg ctctggtcta tcatttactt gattcaacat
     2701 gatatattca aacgtgattc gacggtttgt tttctctaaa tactcatcca cagcttccat
     2761 caatttctca attgggaaac ttcggttgat ccgcatgatc gatgttcgca cctcattatt
     2821 cggcgcgtgc aaggaaatag ccagattgac ttgcaagccg ttattagcga attccttgat
     2881 tttgtgtgct aatccactag tggaaactgt aatgtgccga gcaccaattg ccaatccttt
     2941 ggcatcattg atgatatgca aaaagtccat tacattgtcg taattatcaa atggctcacc
     3001 gatccccatt acaacgacat gtgaaacgcg ttcgccaaga ttgcgctcat caaaataatg
     3061 ttggaccatc atgatctgtg caacaatttc tccagcagtc agatcacggt tctttttgag
     3121 cagcccgctt gcacaaaacg tacagccgat attacatcct acttgtgtcg tcacacaaac
     3181 tgacaagcca tattcttgtc tcatcagtac agtctcaatc atgtttttat cagggagttc
     3241 aaacaaatat tttactgtac catccgaagc ttcttggacg accatctgtt ttagaggatt
     3301 gatgataaaa tgctccgata atttttcgat caattgtttc gatagattcg tcatttctgt
     3361 gaaattactt acacgttttt gatacaacca ttcccaaact tgggcagctc ggaatttttt
     3421 ctcgccgttt tctataaacc aattgattaa ttcttcattc gtcaagccat aaatggactg
     3481 tttttccaaa gtattttccc tctttttctc aaagtttcgc tacatactat acatgattca
     3541 tttccttaat gcaatacttc acaaataaat cgaatagaac tttaatctca caaaaactaa
     3601 aaagacaaga catcataccg tattagaaaa gatggaaaaa aaccattttt tcttttggta
     3661 tcatcatgcc ttgtcttttt attggttatt cgtttttcaa cccttcatca cacaagtttt
     3721 acatgtcatt atcgtccggc aacgtttctg atgtttcttc ctgctcaatg aatactttcc
     3781 gaggcttgct tccttctgac gggccaacaa ctccatgagc ttccagttca tctactaaac
     3841 gtgctgctcg gttgtaaccg atccgaaatc ttctttggag aagggaaata cttgccgttt
     3901 gcatttcaac gacaagtgct tttgcttctt caaacagctc atcttgtggc tgttctggag
     3961 cacctgtcgt ttcaacttca tctgtcggca tcattttctc ttcataatga gcttcttgct
     4021 gatctgtaac aaattgaacg acccgttcta cttcgtggtc agaaatgaat gctccttgca
     4081 cacggatcgg tttattctca cccattggca agaatagcat atctcctcgc cctaagagct
     4141 tttctgcccc attagaatca atgatcgtcc gagaatctgt accgcttgat accgcaaatg
     4201 ccattctgga aggtacattt gctttgatga ttcctgtgat aacatccaca cttggacgtt
     4261 gagtagctag gatcatatga atccccgctg cccgagccat ttgcgctaaa cgaataatcg
     4321 catcttcaac ttcattgctg gctaccatca tcaaatcagc taattcatct acgatcacta
     4381 cgatgaatgg taaggttgga tgtttcgttc cgttttccaa gtttttctgt tgaacaaatt
     4441 cattgtaacc agaaatattt cttactccgg ttgccgcgaa tttttcatat cgttcttcca
     4501 tttcttgtac tactttttgt aaggcttgcg ctgcttttct agggttcgtc acgaccggcg
     4561 tcaacagatg aggtatgccg ttgtacatat tcaattcaac cattttcgga tcgatcatca
     4621 tcagttttac ttcatggggc ttagcacgca ttaatatact tgtgataatc ccattaattg
     4681 ctactgactt cccactacca gtcgaaccag ccacgagtaa gtgaggcatc ttcgaaagat
     4741 cagccgtttg tacccgtcca gaaacatcac gtcctaaagg tacttctagt aatttatctg
     4801 ggtggtttgg ttgtgcttcg atgatttctc taaaggaaac catactgatc tttccatttg
     4861 gtacttcaat accaatcagt gatttacctg gaatcggtgc ttccatccgg acatctttcg
     4921 ccgctaaagc aagcgcgata tcatctgtta agttcacaat tttacttact tttaccccta
     4981 cagccggctg gacttcgaat tttgtgacgg atggtcctaa gctggctttg acgaccttgg
     5041 catcgacacc gaaactttgg aaagtttgtt ctaatacgcc gatatttttt tcaatttttt
     5101 tatactcatc actttgatcc acagttggta tagaatcaag caaatcaact gtcggtaatt
     5161 catagtcttg attttcctgt tctgcttcaa tctcaaattc taatgaggta ccgtcatcct
     5221 ctggcaactc atctgaaaaa ccttctgctg tattggtctg ttttggtgta tctggtactg
     5281 gtttctctag attcgcagga tggatatttt cttgaaaact gtcgattgga acaaaactta
     5341 gctgctccgg ctctgtattt tctggttcct gccattcatc agcagtattt tccctttgct
     5401 ggctcaccgc ttttttcttc tcatattctg cagcttcttt ttctgcagct tcttttgcag
     5461 ctaagcgcct tgcttctttc gcttctgcgc gctgtttcat tctctcttcc tttgccgctt
     5521 gctttcgagc ttgcttggca ggatcacctt ccaataattc ctgtactcgt tctccaattg
     5581 attggatacc atttaacagc tgatagcctt ccagattact gaacaggaat gctcctcctg
     5641 ccagtaatag cactgctgct aaataacttc caggctgtgc aatcaagaag taagtaaaat
     5701 gataaaggat tgctccaatc atcccaccac caacgttatt gtctacttga ctttgtttta
     5761 tatcactggc taacaaatcc catgtcgtcc ccatgatatt tggttcccca gtatggagtt
     5821 tgccaaataa atgggcatgt aacaataata agataccgaa ataaaagagt atgccgccaa
     5881 accagcgtcg gtttttacca attgaaaact ctgtattctt aatgacaatc caaaatccta
     5941 atacggctaa taaaagacag aggatttggt aagtatttcc tgcaatgatc cgaaagccgt
     6001 ttgcaatcaa tattcctaaa aaacctaatt ttaaaaagcc gaagacagca aataaaatca
     6061 aggcaaaccc aacaccaatc gtcagcattt gttcctgctg ctgtttttgc tttttcgttt
     6121 ttttcgtctt tttccttttt ttagtaggcc gtttcttttg tgccatttat tatccctcat
     6181 ttcatcaagt ttcattatac aaaaaaaagt aagggaacta aagttttgtt tgaaagaact
     6241 gacgctttct ttcatttttc agcttatttt acagtttttc ttaatttcta aatgatttta
     6301 tcaagaaaag gcacggccat atccagccgt accttcttgt tttaagtatg tgaactaaag
     6361 gttctctcca ttttcttcaa tcagtttttg gtaccagtaa taactgtctt tcttatatcg
     6421 ttccaaagat ccttcttgtg tttcgtcttg atcaacatat acaaatccat atcgcttttg
     6481 ataaccgttc agccagctaa gcaaatctgt ataagaccag gtgcaatagc ctaggacttc
     6541 tgctccatcc gaaattgctt ctttgatggc tttgacgtgg ctttgtaagt aattgattcg
     6601 atagtcgtca tggatttgtt tatcttctgt caacttgtca tactctccta agccattttc
     6661 cgtaatcagt accggcatgc ggtaacggct tgtgatacga cgcagtccga tccttaatcc
     6721 ttctgggtca atctcccagt cccaatttgt tcgttcgaca aatggattct caactttttt
     6781 atacattcca ggaactccag tttcttctga tgaacctttt tttcctgtcg tattcatttt
     6841 tccagcacca actccgttta atggattgaa tgcattcgtc gccgtttgat agtaattgat
     6901 cccaagaaaa tctggtttag cagattctag caattctgca tcgccagctt caatcgttgg
     6961 tgcccatcct ttttcttcta agaacctcat ggcagcaatc ggatattttc cccacatata
     7021 gacatccatc caataattgg ccatcaggtc ttctgcattt tcagcagcca gaacattttc
     7081 aggattactg tcaattggat aatttggcgt atacgcaaag cttggtccga tttttccagg
     7141 catttctaac tcgtgaaatt tattgatgac agatgcattt gctaaattag caatatggtt
     7201 aacttcatac attcgtttcg ggtctgtcac acctggcgga tgtgctgcta acaaataacc
     7261 taatgaagtg aagatatttt gttcattcaa actaatccag taatggactt tcccgcggaa
     7321 tgcctcaaat aatgtttctg cataatgagt gaaatcttca atgatctgac gtgattccca
     7381 accattatat tcatcttgca atgcttgtgg aagatcccaa tgatataaag tcaacatagg
     7441 ttcaatatcg ttttcaatca attcgtcgat caatctctca taaaaagcga gcccagcttg
     7501 attgacttct cctcgtccat taggaaaaat ccgtgtccaa gctacagaga agcggtaagc
     7561 tttcagacct tgttctttca ttaaagcaat atcttcttta taacgatgat agtggtctac
     7621 tgccaaatca ccgttcgttc ctttaaatgt ctttccaggt atccgtacaa atcgatccca
     7681 gactgattct cctttcccat cttcttgcca agctccttct acttgatacg ctgcagaagc
     7741 agagccccac agaaaatctt ttggaaatga ggtaagttct ttgtgttcca ttcatctctt
     7801 cctttcctat aaccttcaat cgttcctctt ttagaataac acgaaacccc ttccacaaca
     7861 aaataaaatt atgtaaagga ggttgtgaaa aagtcgctta gtttcgagta
//
LOCUS       DACEPD010000104         7780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.109, whole genome shotgun sequence.
ACCESSION   DACEPD010000104 DACEPD010000000
VERSION     DACEPD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.109"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            79..1161
                     /locus_tag="IW797_002388"
     CDS             79..1161
                     /locus_tag="IW797_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ1225147.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            1161..1964
                     /locus_tag="IW797_002389"
     CDS             1161..1964
                     /locus_tag="IW797_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1225148.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            2034..2774
                     /locus_tag="IW797_002390"
     CDS             2034..2774
                     /locus_tag="IW797_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ1225149.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            2785..3789
                     /locus_tag="IW797_002391"
     CDS             2785..3789
                     /locus_tag="IW797_002391"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ1225150.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(3834..5678)
                     /locus_tag="IW797_002392"
     CDS             complement(3834..5678)
                     /locus_tag="IW797_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225151.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLIHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            5890..6408
                     /locus_tag="IW797_002393"
     CDS             5890..6408
                     /locus_tag="IW797_002393"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1225152.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            6536..7219
                     /locus_tag="IW797_002394"
     CDS             6536..7219
                     /locus_tag="IW797_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ1225153.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            7528..7601
                     /locus_tag="IW797_002395"
     tRNA            7528..7601
                     /locus_tag="IW797_002395"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7562..7564,aa:Arg,seq:tct)
BASE COUNT         2571 a         1250 c         1563 g         2396 t
ORIGIN      
        1 aaaataccaa gtttgcctga acaaaaatca taagcaatgg tataatcaca atatgaatat
       61 agaaatgggg gaaaacattt ggatacaaaa gaagaacagc tacttattga tttaacttct
      121 gcaaaaggag tacctggaaa tgaagaagag gtaagagaag tattcagaga atatgccaaa
      181 ccattcgctg atgatatttt ttatgacggt ttaggcagtg tgattgccaa acatggtgct
      241 ggcggaggcc ccaaggtctt tatttctgga catatggacg aagttggttt tatggtaaca
      301 aaaataaccg aaaaaggatt cttggaattc caaactcttg gaggttggtg ggggcaagtc
      361 atgcttgctc agcaagttga aatcaaaaca cgtgaaggga aaatcgtcca cggtgtgatt
      421 ggttccaaac caccacatgt tttaacacct caagtaagaa ataaacctta tgaaataaag
      481 gatatgttca ttgatattgg cgcatcaagc caagaagaag ccaaagagtg gggcatacgt
      541 cctggagata tggtcactcc atatattgaa tacaaacgaa tgaatggcag taaatattta
      601 ctggcaaaag catgggataa tcgtatcggg acagctgttt ctctgcgagt attagagaat
      661 ctatctaaag aagcacaccc aaatgtttta ttcgcaggaa gcgatgtgca agaagaagta
      721 ggcttgcgag gtgctcgaac aagtacacac cttgtaaatc ccgatattgc ttttgcttta
      781 gatactggta cagcaggaga cactccagga atgacaccaa aagaagcaga ttcgatttta
      841 ggaaaaggcc cacagatttt gatttttgat gcatctatga tcccgcataa aaaattattg
      901 aattttgtta ttgatgtagc agaggaaatg gatattccat tccaatatac agtgatttct
      961 ggcggaggaa ctgatgcagg acaaatgcat ttgacaagag atggtgttcc gtctgtagcc
     1021 attaccgttc ctgttcgtta tctgcattcc catacatcag tgattcatga agatgattat
     1081 ttgaatacag tgaaactagt cacagaatta gtgaaacggc ttgatagtga aacagttgaa
     1141 aaaattcgga gttatgaata atgaataata gaatacctat ttctgaatat cggaaaaaag
     1201 cacgagtttc tttggaaaat caatggggaa tcaatacttg gcttgttttc ttatctataa
     1261 tatttacgag cgtcatccag aacttttttg acggtttcag tccttttccg gaagaaagca
     1321 tgcagcagca cttattgagt tttctagtcg ataatttact tttattcgca ttcaactatg
     1381 cattgtttta cattgcgcta gtagttgtac ggggtggcag accaaaaagt aaccttttat
     1441 tttcagtttt ccaaagaaag tattttggtt cgatacttgg catcaatttg atcaattcag
     1501 tcatcaactg gctactcaac agtttaatct ttctgcccat gtttttaatc agcggagtga
     1561 gtacttatat gcagctgctt ttcagttcaa atgcggtacc tacgggtgtc atgccagaag
     1621 attttcttga tatcagcttt gttttatcga tgatagggat gatttttgtt tcagcactca
     1681 tcatgagtat tgttggcggt ttattccaat ttgctgcatg gacaaagttc gactatcctg
     1741 atttgtcggt tattcaatgt ttgaaatatg gctggttttt attgaaagat agaattggta
     1801 cgtatatttt gttgcagctg tcttttatcg gctggtattt gctaggagca atcgctttgt
     1861 tcatcggtct tttgtgggtc attgtttatg tgaatgtgtc agttgcagaa ttttatgaac
     1921 aagcacggat tgaaaaagga ggtccagacg cctattttac ttagcatgta agcagtttat
     1981 tcatcaaaaa aataactttt gtggtaggat aaatctttga gggggaatgt acgatgaaat
     2041 taactgtttt aggatgctta ggagcgtatc cttataaaaa tcaagggaca acaggttatt
     2101 tgcttcaatc tgacgggttc aatttgttga tcgatgcagg tagtgctacg ctgatcaaat
     2161 tacaagaaca ccttgatcca cttgatctag atgctgtgat ccttagccat tatcatcatg
     2221 atcatattgc tgaccttggt gtattacagt attactggca acttcatcct aagagaaatc
     2281 cggagacatt gctttcaatt tatggacaca caaaagattc cattcacttt gaagaattga
     2341 cgttggaaaa tgtctcaaaa ggacatcctt attttgaagc tgaagaatta aaaattggtc
     2401 cttttctgat tacttttatg gaaacaatcc atccagtagt atgttacgcc atgcgtatcg
     2461 tagaagaaac aacggggaaa gtattcgtat ttacaggtga ttcaggttat ttggaaagtt
     2521 tcgctgattt tgcgaaagat gctgatttgt ttctagcaga tacctatctt tttgaaggaa
     2581 atgaacatca tcatgcccat ttcacttcaa aagaagctgg agaacttgcg aaatcagcta
     2641 acgtaaagaa acttgtactt acacacctac ctcagtttgg tgatttagaa caattgaaaa
     2701 atgaagcgca aaaggcagca ggcaaaggga ttgatataga acttgctgca gttgacaaag
     2761 tgtttaacat ttaggaggaa gaacatgatt tttgtaccaa atgaaaacca cgacccaaga
     2821 atcaatttag caattgaaca atatttgctg caagaaatgc ctttagatga acctattttg
     2881 ctcttttata tcaatgaacc ttcaatcatc atcggcagaa accaaaatac gcttgaagaa
     2941 atcaatcgag attatgtgga agaaaatggc attcatgttg tccgtcgctt aagtggaggc
     3001 ggagctgtct accatgattt tggaaacctg aactttagtt ttatcatgcc tgatgatggg
     3061 gattcattcc gtgactttgc gaaagtaaca cagccaatca tccaagcact tcatgaattg
     3121 ggtgttgctg gggcggaatt gaaaggccga aatgatctag tcattgatgg catgaaattt
     3181 tctggcaacg cgatgtatgc taccaacggt cggatgtttg ctcatggaac aattatgttc
     3241 gactctgata tcaatgaagt agtcaatgca ttaaaagtaa aaaaagacaa aatcgaatca
     3301 aaaggaatca aatcgatccg ttctcgtgta acgaatatca aaccatttct tccagaagaa
     3361 aagcaaaaaa tgacaacaga agaatttcgt caagaaatct tgttgaaaat tttcggcgta
     3421 gagtcagtag aagaagtaaa aacgtacgaa ttgactgatg aagattggaa aaaaatcaat
     3481 cgaatctctg aggaatatta tcgaaattgg gattggaatt acgggaagtc tccagatttc
     3541 aatttctctc gccaaaaacg tttttctatc ggttcgattg aagtccattt gaatgtctca
     3601 gaaggaatga tcaaagaagc aaaaatcttt ggcgatttct tcggactagg agatatctct
     3661 gacgtggaaa aaaaattggt tggacagaaa tatgataaag catctctatc aaaaatagtc
     3721 agccaaattg atgtgaagaa atattttggt gcgatagaac cagaagaatt gcttacctta
     3781 ctttattgaa aataaaaaat agcggaatcc acatatatgg attccgctat tttttataca
     3841 ggattcgggt aatttcgaga gagttgaaca gtgatccgat caatgaaagt atctttttcc
     3901 tgcgttagtg ttcctgtgta acgatccgct acgtctttca tcatcattaa gtcaaaacgt
     3961 tcctgatcat ctaattcttc tgtgatgcct cgactaaata ggtttgtatc gtaatgtgca
     4021 ctatgttcgc aacaaatgac gaaatgttcc tcttgttcgt gtatcgacaa ataaattttt
     4081 cgatacagtg gattttcgat ggcatgagtt tctctaaagg aatgctctaa agcgtggatc
     4141 aaaagttgag ccagatcgtc atcgttgata ggaagttctt cgggaagatc tgcttggaag
     4201 gttatctgaa tattccgttt ggcagctaac ttctggtaac gcgctaagat aagatttgtc
     4261 acctggtggt tgcaaaaaga acacagcatt tcgaaatttt ttgcttcttc taatatcttt
     4321 tccacatagt ttttagcttt tgtttggtcc gtatgtatta aatcttgcag attctccatc
     4381 tcttggacaa attccaggcg aagtgtattt acttcttgga tatgctgtcg aagctgctca
     4441 taatgttgct ccaacaaatc atttttgatt tttaagaaat ctacttgtct ccgatctttg
     4501 tcggtattta agatgtgatc gattagattg tagctgataa taacgattag aatgataata
     4561 tataaaatcg tagaccaatt gatcgttgtt tgatagatac ctaatgccgt ttggatgtaa
     4621 atcaagcaat ggataatggc taccaagacg atccaaggtg tacaagctac ataaaaacgg
     4681 ttattcttat atgcttcgcc aatgcatgca agtgaagtga ctaaagtgct tataacacta
     4741 gcaatattag caatcggcat taattcagga agttctgcag gagtaaaagt tgcataaagc
     4801 agaacgccga taaaaatcaa aagttggaac gtacaaaaaa ttccgtacca ttttttatag
     4861 taaaacattc ggctccaata gtagcagatg atgaaagctg acgttaagat aataaataaa
     4921 tgagataaaa aagaatgaac gaatggttca aaaagaatac cacttaaaat atcttctgat
     4981 acagcttcaa atcctaatgc caaagcaaag caacttaaga gaataagtga gtattcaaga
     5041 cgtttttgtt tatacatcaa gtagatcgta tagaaaaagg tccctatagc gataaaaaca
     5101 gcgattatca tcgtcaatac ttgcggcact gattgtgaat aaataaaact aatcaatggt
     5161 ccagttgttc ctagataaac tttcggtggc agtccagcat agttctgata aggagatgag
     5221 actgtaaggg tcaatttttt tccgatataa tttgctggta aatctatcac agccaatgag
     5281 agtccgggat tgctttcttc ctgataatct ttccgttggt ataaaatctt ttcgcctaga
     5341 tatacagtaa tccattgatg gtttcctttg attagtaaat ttgcttcgtc caacggtttt
     5401 tcaagtgtcg tttccataat gaatgtttcg tctttatcca cggtaggaat cgtacgagtg
     5461 tacttgctgt caaacttaaa atcaggatca tcttttgtat agaaaaccca cgaatctttt
     5521 atttctttca tttgattgct gtccgcagga gaaaaaataa aataaaagaa ataagcaatt
     5581 cctgttaaaa ataacaatga taatataatg attatgctcc actttatacg taaataaaaa
     5641 gattcttttt tattatatat gttattaaaa aaagacataa aggtattcct ttctaaaact
     5701 tgtaatgtta gggtaaacgt tttttaaact aacgtcaatt atttataata aaaaatgaga
     5761 taaagatgag gaaatagaat tagaaacata taattattaa agggaaacat ttttacagct
     5821 ataatttgaa aaaaatttat cgttattata tgattaagaa ggattgaata aagaaagaag
     5881 ttgaaaacga tacctgatta caaaaaaata acttttataa gttctataac gttgattata
     5941 tttttaattc ttattcttct cgtcgtcttg gttccccgaa tttttggttt gactccttat
     6001 attattgagg acaaagaaat gagtccatat tatgcacaag gaaatctgat atatgtacga
     6061 ccgaaaaata caaatgagat tttagtcgga gatattatta catattatga aaattcagga
     6121 aaaaaaatct cgacgcgtcg cgtaatagcc gttgaaaaca accacgaaga tttttataca
     6181 aaagctgata caaagactta tattgagcct ggagtagtaa aaaagagaaa tattatcggt
     6241 agtcccatct ttcagattcc atatattgga ctagtccttt caaaaacggc atttgcatgg
     6301 attcaggggt tattctttac gattgctttt tgcctaacag gaatcacagc atggaatacc
     6361 tttgtagagt tgaaaaagaa gcggagacga acacagaaat ttttgtaaga atgttaaaaa
     6421 cataaatgta ttccagagat ccaacgactc ttctgaacat gaaacacgag aaaacactca
     6481 tgcagcttgt agatttagtc agttgttcgt ataatgtgtg aagaggagga gtagtatgcg
     6541 caatgaaatg atgcatcgtc ctgtagtcaa agaagaacta ttggatttta tgcgtaccca
     6601 gcaaaaaaaa ttaccgggtg agctaggcaa actggaagaa gaagctcatg tagcagaagt
     6661 accagttatt ccccatgaaa cagttgtatt tttgaaattt ttacttggac agcttcagcc
     6721 tgaacgaatc ttggagatcg gagcagctat cggtttttca tcaagtgtca tggccacgac
     6781 aatcggagaa aacgctcatg ttacgacaat tgatcgtttc gatgtcatga tcgagaaagc
     6841 taaaaaaaac tatgaacgtc taggactgac agataaagtg actttgcttg aaggtcaagc
     6901 agcagatatc ttgccggagt tgtctggtcc gtatgacttt atttttatgg atagtgcaaa
     6961 atcaaaatat attgaatttt tacctgaatg cttacgtttg ttaagaaaag gtggcgtttt
     7021 gatggtagat gacatcttcc aaggaggaac gatcttgctc ccagatgaag agattccccg
     7081 tggcaaacga gctatccatc gtaaattaaa tgaatttctt cgtgttgtga tggatcatcc
     7141 cgatttgaca tcgacaatct tgccattagg tgatggtgtg atcttaatga caaaggaatc
     7201 agaaacaatc gagttgtgat acatgattat ggcagcctgt agataatcga ttgaatcgag
     7261 ggctcaattc gaacttgatg taaaagtttc tatatttgta agtcaattaa aggatacagg
     7321 aaatctaaat aaacaggagt agcatgaagt caatgtagct cctgttttca attgataacc
     7381 ttagaacagc tgaaggagaa tgtctcgttt taaaagattg atttagtagt tgggccaaaa
     7441 taattcatta tttgctaatt aaataactat ttcgtattga cagaagcaat ttaattatct
     7501 ataattattg atgttccgct gattcttgtc atagtagctc agctggatag agcatccgcc
     7561 ttctaagcgg acggtcgggg gttcgaatcc ctcctgtgac gctaaaaaac cgttagaaac
     7621 tgaggtttct aacggttttt ctttgttttt tgtgccaata ttgtttgttt tcaaaggcaa
     7681 ataaaataaa aaaacatcct tatcgagtac aattataagg agcaaaaaaa taaaactcat
     7741 cagaaaagag gaagaagata tgacgatact atctagaatt
//
LOCUS       DACEPD010000105         7599 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.110, whole genome shotgun sequence.
ACCESSION   DACEPD010000105 DACEPD010000000
VERSION     DACEPD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.110"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(138..428)
                     /locus_tag="IW797_002396"
     CDS             complement(138..428)
                     /locus_tag="IW797_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225154.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IW797_002397"
     CDS             complement(546..806)
                     /locus_tag="IW797_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225155.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IW797_002398"
     CDS             complement(943..1356)
                     /locus_tag="IW797_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ1225156.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IW797_002399"
     CDS             complement(1812..2297)
                     /locus_tag="IW797_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225157.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2681)
                     /locus_tag="IW797_002400"
     CDS             complement(2436..2681)
                     /locus_tag="IW797_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ1225158.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="IW797_002401"
     CDS             2874..3674
                     /locus_tag="IW797_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1225159.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="IW797_002402"
     CDS             3693..5561
                     /locus_tag="IW797_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1225160.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRSYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="IW797_002403"
     CDS             5554..6045
                     /locus_tag="IW797_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ1225161.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /gene="srlA"
                     /locus_tag="IW797_002404"
     CDS             6033..6587
                     /gene="srlA"
                     /locus_tag="IW797_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ1225162.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..>7599
                     /locus_tag="IW797_002405"
     CDS             6605..>7599
                     /locus_tag="IW797_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ1225163.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1214 c         1442 g         2236 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aagctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gttcccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctg
//
LOCUS       DACEPD010000106         7480 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.112, whole genome shotgun sequence.
ACCESSION   DACEPD010000106 DACEPD010000000
VERSION     DACEPD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.112"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(498..1844)
                     /gene="gor"
                     /locus_tag="IW797_002406"
     CDS             complement(498..1844)
                     /gene="gor"
                     /locus_tag="IW797_002406"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ1225164.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(1978..3219)
                     /locus_tag="IW797_002407"
     CDS             complement(1978..3219)
                     /locus_tag="IW797_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1225165.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3237..4160)
                     /locus_tag="IW797_002408"
     CDS             complement(3237..4160)
                     /locus_tag="IW797_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ1225166.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4381..6873)
                     /locus_tag="IW797_002409"
     CDS             complement(4381..6873)
                     /locus_tag="IW797_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ1225167.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6880..7347)
                     /locus_tag="IW797_002410"
     CDS             complement(6880..7347)
                     /locus_tag="IW797_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ1225168.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2054 a         1631 c         1198 g         2597 t
ORIGIN      
        1 tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacacta tttgttcgga
       61 aaaagtgact aactatgaca caattttttt cacagtccct tttttcactt gatttgattc
      121 gtaccagcga acttgacgag aactttcttg gtttggtaac tatatggtat tcttttaaca
      181 tcctcgtttg cttcttctaa tttatttata caaagtcaca cttttttaaa catctgttct
      241 tcgatacatt tctatttctt ttaaacatcg ataccaatat tttgaactta agagtgcata
      301 ccaaatggat tcatttactc ttcgtcagtt acgaagataa tcaaaatctc caacttccct
      361 acttatttcg ttttgagaat tatcgaagca atcttacata aataactaaa aaacaaaaaa
      421 ccagaaaatc agtatactga ttttctggtt ttacatggtt tgttcaataa aaagctgttg
      481 gaactgttaa tattaaatta tcgcatcgtt acgaattctt ccgctcctgt tggatggatg
      541 gctactgtat tgtcaaagtc ttctttagtt gcacccatct tgatagccac ggcaaaacct
      601 tgcagcatct catcgacacc aacaccaata gcatgtaagc cgacgattct ttcttctttt
      661 ccgacacaga tcaatttcat ttcgcatttt tggcgataat catttaatgc aaagtacatt
      721 ggggtaaagc gtgaacgata aactttcaat tcattttcgc catatttttc caatgcttct
      781 tcttctgtca aaccaattgt tgcaattggt ggatgcgtaa agacgactgt cggaacaagt
      841 ttatagtcaa gataagaatc tttctttcca ttaaataaac gttctgataa acgtctacca
      901 gctgcgattg caacaggagt cagatctaat ttaccgatca catcaccaac agcaaaaata
      961 tgttcatccg ttgtattttg gtatttatct actttgataa accctttttt gtccaattcc
     1021 acatttgtat tttccagacc taatttttcg gtattcggta cacgtccacc ggcaaaaagt
     1081 acacaatcac cagttaaggt ttcaccattt tcaaatagca ccgtatattc gtcattgttt
     1141 ttagtaattt ctttagcaat atgatgtgcg taagttttta ttccagcttc ttggtacatt
     1201 tggatcaaat tgtccgaaag cattttatca aacgttctaa gtggtcgttc ttttcgatat
     1261 gcccacatga cttcactacc taaaccacga aatacacctg aaagttcagc cgcaatgtat
     1321 ccagcaccta aaacgattgc tctcttaggt aactctgtta attcaaaaaa tccatttgaa
     1381 tcaattgcgt attctccacc aggaatggtc attttacttg cacggcctcc agtagcgatc
     1441 aagattttag gagcacgata ggtttcaccg tttacttcga tcgtttgact atcgataaaa
     1501 gtagcgtaac cttttatagc ctcgacatga ttgctatcta atcctctttg ataagcaccg
     1561 tgtagaaaat caatatattt ttctcgtctt tctactagac cggcgaaatc aacatttttt
     1621 aaatctgctt ggataccgta acttgaggca tctcgttcaa ttgtttccaa aatcgtactt
     1681 gcttgccaca taactttttt aggtacacaa ccgacgttga cacatgttcc gcccaattca
     1741 ttcgcttcaa tcagcaaaac ttttgcgcca tgcatccctg ctctgtttgc tgaagcaata
     1801 ccgccagagc cgccaccaat tacgatgtaa tcatatattt gcattttgtt cctccttatt
     1861 gaagagatca atcaaaacct ttatttaacg atcctttttc tacagaaaac aagtgtaaca
     1921 cttttcttga attttgtatg cgaagatgct taaaacaggg atatatcagc tttttcctta
     1981 tttacgtttt cggatcatat ctccaggttt caccggctgt gcgactggca tagttaaaat
     2041 catcattgga tttggtgctc ggtcaatgga ttcgccttct tcattatgca taacttcaac
     2101 ggtttgactg aaatgactga atcctggacc ataaaattcg atttcatctc ctacagaaaa
     2161 caagtttctc tgacgtatcg tagcaatctg tgttgcctca tcataagata atacttcacc
     2221 gacaaattta tatactggga ttttacgacg ttcaccgaat aattgttcgt tttcagttgg
     2281 tgtattgtag taaaaacctg tagctaattc tcgttgtgct actttccata actcgtcgat
     2341 ccattcttgc ttgcagacat agttttcagg atcttccata taactgtcta cagccgcttt
     2401 atagacattt gacaccgtag atacataatg gatcgatttc attcttcctt cgatcttgaa
     2461 gctgtctacg ccgttttcaa tcaattctgg aatatgttcg atcattgaca tatccactgc
     2521 actcatcgag aattcttctt ccacttcgcc tgtatcagtt aaactgtttc gttcttgacc
     2581 aaaaggcata tcgaacaatc catacttcca gcgacaagat tgagaacatc cgccacggtt
     2641 tgcatcccgc atcgacatat ggttggacaa tgtgcatcga ccagaataag agatacacat
     2701 ggcaccatgg ataaatgctt cgatttcggt atccgtattt ttacgaatct ctgccacttc
     2761 ctccattgaa acttcacgag ctaaaacgac acgttccaac ccttcttctt tccagaattc
     2821 caatgtctca aaattagttg ctgaagcttg agtggataaa tggatcggca gacctggggc
     2881 ttctgtcgca caaatttcga tcaaagctgg atcagaaaca atcactgcag aaataccgac
     2941 atcacgcaaa ctgcggaaaa attcccctgc tccatcttca tttccttcat gggtgaccat
     3001 attagcagca acgtatacct tcgcatcatg ttcttttgca aaagcaactc cttcagccat
     3061 ttcttcataa gtaaaattac ctgcacggct tcttaaacca tatgcattcc cgccaatata
     3121 tactgcatct gctccataat ggatcgcggt ttttaacttt tccagtgttc ctgctggcgc
     3181 taggatttct ggtcgtttca attttctttt tactgtcatt tctttcatca tactccttat
     3241 ttgatagcag aaggatcaat ataaaagaat cctgtatcta attcacggtt ttcaggatgc
     3301 aatttactga tttcgctagt cattttttcc gctttttctt gatcccagct accttctaaa
     3361 atttgttttt ttgcttcggc gaatagtcga gtgatgttta cgaatttttc gcctggagta
     3421 aataatccgt ccaatttcca tgtacagtaa tgatgctgat aaagttcatt caattcattc
     3481 gagagattca gatcattgct ggcaaaaata tgtgtgccat gactatcttc ataaatagaa
     3541 taatgtgtct cttctttttt aggttccgag ataaaaagac ctctttcacg atctttttgt
     3601 tcgtcttgtt tcgtatagtt atagtaattc tgtagcagtg gacgtttgga ttgatggatg
     3661 catgtagcac catacacaag ggtttctaca ggaatatcga gtttttcttc catcgccttc
     3721 atctcttcaa aaggaacttc acgagcaagt actgctccaa ctgcgccttt tttcgcccag
     3781 aaattgattt gacgtgcgct cgttactaat gtttcagcat cataaatata cggtatacgc
     3841 aattcaggat tttttttcat cgtataaata atacctgtat caccaactgt gatcacatct
     3901 acgtttattt cttttaaaaa agccaaatat tcaggtacat ttttcatttt ttcaggatga
     3961 atgatcccat ttacagcaat attcgctttt ttaccatact tgtgtgctaa ttctaccaat
     4021 tgtcgctgtt cttctcgtgt aaatgaagca ggcagacgca atccaaattg ctcttcaccg
     4081 aaatatagtg tatctgcacc tgcagcaagc aactgttctg cttgttcaat agtttctact
     4141 gtggttatga gttcgatcat aattttctcc tcctacaact tcataatagt agcatagctc
     4201 taaaaacttt ccaatacttc ttctatttaa agtcggattt ttgaaaatta tttcacaaaa
     4261 aatcaaaata aagttcgata tattctgtcc aaatataaaa agagattgtg acaaaaatct
     4321 tgtcacaacc tcttaatcaa gtcttactat tataaaaatt cagctacaca ggccaagtaa
     4381 ctatagggaa cctactgttt cctttttagg tccgcgaaca ttcaatgtga tcttaccttt
     4441 tgaagcacct actgttacgt ggtcacctaa acgaatttct ccgccaagca aagcctcgct
     4501 caatcgatct tcaatttctt tttgaagtgc gcgacggatt ggacgtgcac catattcagg
     4561 atcaaatcca gctttcccga tcacatcgat tgcagatggt gtgattttca cttgaatatc
     4621 ttgttctttc aatcgtttga taatcgcttt gctcatgatt tggacgattg tatggatttc
     4681 atctttactc aatgaatgga agacgacagt ctcatcgatt cgattcaaga attcaggtcg
     4741 gaaagctttc tttaactctt ctaagatacg tttttgcatg gctttgtgat ctttagtaac
     4801 atcttggaca ttgaatccga catttttttc ttctcgaatt tgtgtcgctc cgatatttga
     4861 agtcataatc atgattgtat ttctaaagtc cacttttcta cctttagaat ctgttaaatg
     4921 accatcatcc aatacttgca gaagtaaatt gaaaacgtct ggatgtgctt tctccacttc
     4981 atctaagaga atcactgaat atggtttttg acgaattttt tctgtcaact gtccaccttc
     5041 atcataccct acgtatcctg gaggagaccc gattaatcgg cttgtactgt atttttccat
     5101 aaattctgac atatcaacac ggatcagggc atcttcactt ccaaacatga cttctgaaag
     5161 tgctttggcc aattctgttt ttccgacacc agttggtcca aggaacatga atgaaccaat
     5221 tggacgatca ggatctttca atccgcttcg tgcacggcgg atcgcacgtg aaactgcttt
     5281 gacagcctca tcttgaccaa cgacacgttc atgcagaagt ccttccagtt ctaacaagcg
     5341 ttcgctttct tttttctcca actgctgtag agggacacca gtccactccg atacaactgt
     5401 agcaacatct tcttctgtta cacgatcaga ataaccttgc tcctgttttg cttcaattgc
     5461 agaaactttt tgcagctttt tacgtagtgc tttttcttgt ctgcgtaatt gagcagcatt
     5521 ttcaaagtct tgtttttgga tcgctgcttc cttttcttgt actagttcat cgatttctag
     5581 tttgatcaca gttgattttg ttaagtgatc ggtttgatcc aaacggactt tcgctgctga
     5641 ttcatcgatc aaatcaattg ctttatcggg tagttgacga gaagtgatat aacgaacaga
     5701 aagattgact gctgctcgta cagcttcatc tgtgatctcc actccatggt gctcttcata
     5761 tcttgtacgt aatccttgaa gaatcacttc tgcttcttca ggagttggct catctacttg
     5821 aatgcgagca aaacgtcgct ctaatgcaga atctttttcg atatattttt gatattcatc
     5881 tagggtcgtt gctccgatcg tttgtaattc tcctcgagct aacgccggtt tcaagatatt
     5941 cgaagcatca attgctcctt ctgctccacc tgcaccaatt aaggtatgca attcatcaat
     6001 gaataagatt acttgaccat cattatagat ttcgtcgatg acctttttca ttcgatcttc
     6061 aaattcaccg cgatatttcg taccagcaac caaagatccc atatcaagca tcatcaggcg
     6121 cttttcttgc atgtcttctg gcacttcacc taaaatgatt ttttgagcta acccttcagc
     6181 aatggctgtt ttgcctacac caggttcacc caccagtaca gggttattct ttgtccgtcg
     6241 gctaagaatc tggataagcc gtttcacttc tctcgaacga ccaacaactg ggtccagctt
     6301 gttttctttt gctaatttcg tcaaatcacg tgctaatgaa tctagcgtag gtgttccttg
     6361 cggtgtttgc cgtttatctt gtacatttac atttctacga cgattcattc cttttccagc
     6421 agaatcacca gctagcccca ttttttgttt cagaacttga cgggcttttg aaaggctgat
     6481 acctaagttg atcatgattc gagtagccat gatactttca tcttttaata agctcatcaa
     6541 aatatgttct gttccaactt tttgagaacc agttttcttc acttcgctgc tagctaaagc
     6601 aaaaacttgt ttcattcttg gtgaatatgg aagaaagact cctttagggt aagaacggat
     6661 accaccataa ccaatcagat gttcaatttc ttcataaatt tccgtttcat tcaaatccat
     6721 ttcacgtaat acttttccag caattccatt ttgctcgacc actaaggcta gtaaaacatg
     6781 ttctgaacta actgattgat gtttcaaccg ttttgcttct tcttgtgcaa tttctaatac
     6841 tgcttgggct ctttcagtaa aaagttcttc catctaatct cacttcccat cttcataact
     6901 taaacgttcc agaaatgctc ggagaatccg agcacgtgta tgatcttcat attcattgaa
     6961 attcaatgtg ttttttgcaa ttgcactcaa catcaagttt ccttcttttt ttgtaattat
     7021 ttggtcttca taaagttttt gaataatagc aaaagcattt ttctcactga ttgtatcatc
     7081 aaataactga tttatctgct caagtaattg ttttttatct gaaatcctta cttttgcaat
     7141 tcgtatataa ccaccaccac cacgtttgct ttctactaga taaccacgtt ggatggtaaa
     7201 acgggtatta atcacatagt tgatttgtga aggaacacag ttaaagaggt ctgccatttc
     7261 aatccttcga atctcgatca tttcactatt ttccagaatt ttctttatat atgattcaat
     7321 taaatccgag gtattctgat gtgccatctt attcattccc tttgactaat attgacctta
     7381 attataacga atttgataat agaaagaaag aagaacgctt tttgttaaat aaatccttta
     7441 gaatcaacaa aaaaaaatac ggaatatact attccgtatc
//
LOCUS       DACEPD010000107         7403 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.113, whole genome shotgun sequence.
ACCESSION   DACEPD010000107 DACEPD010000000
VERSION     DACEPD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.113"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            71..154
                     /locus_tag="IW797_002411"
     CDS             71..154
                     /locus_tag="IW797_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1225169.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            279..1016
                     /gene="erm(B)"
                     /locus_tag="IW797_002412"
     CDS             279..1016
                     /gene="erm(B)"
                     /locus_tag="IW797_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1225170.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1186..1446
                     /locus_tag="IW797_002413"
                     /pseudo
     CDS             1186..1446
                     /locus_tag="IW797_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1609..2274
                     /locus_tag="IW797_002414"
                     /pseudo
     CDS             1609..2274
                     /locus_tag="IW797_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2310..2555
                     /locus_tag="IW797_002415"
     CDS             2310..2555
                     /locus_tag="IW797_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1225171.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2658..3527
                     /locus_tag="IW797_002416"
     CDS             2658..3527
                     /locus_tag="IW797_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1225172.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3508..4242
                     /locus_tag="IW797_002417"
     CDS             3508..4242
                     /locus_tag="IW797_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1225173.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4275..5187
                     /gene="ant(6)"
                     /locus_tag="IW797_002418"
                     /pseudo
     CDS             4275..5187
                     /gene="ant(6)"
                     /locus_tag="IW797_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5184..5726
                     /gene="sat4"
                     /locus_tag="IW797_002419"
     CDS             5184..5726
                     /gene="sat4"
                     /locus_tag="IW797_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1225174.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5819..6613
                     /locus_tag="IW797_002420"
                     /note="aph(3')-IIIa"
     CDS             5819..6613
                     /locus_tag="IW797_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1225175.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6721..6909)
                     /locus_tag="IW797_002421"
                     /pseudo
     CDS             complement(6721..6909)
                     /locus_tag="IW797_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6889..>7403
                     /locus_tag="IW797_002422"
     CDS             6889..>7403
                     /locus_tag="IW797_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1225176.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMI"
BASE COUNT         2593 a         1119 c         1555 g         2136 t
ORIGIN      
        1 ttttgtgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga
       61 gggattcgtc atgttggtat tccaaatgcg taatgtagat aaaacatcta ctgttttgaa
      121 acagactaaa aacagtgatt acgcagataa ataaatacgt tagattaatt cctaccagtg
      181 actaatctta tgacttttta aacagataac taaaattaca aacaaatcgt ttaacttctg
      241 tatttattta cagatgtaat aacttcagga gtgattacat gaacaaaaat ataaaatatt
      301 ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat aataaaacaa ttgaatttaa
      361 aagaaaccga taccgtttac gaaattggaa caggtaaagg gcatttaacg acgaaactgg
      421 ctaaaataag taaacaggta acgtctattg aattagacag tcatctattc aacttatcgt
      481 cagaaaaatt aaaactgaac attcgtgtca ctttaattca ccaagatatt ctacagtttc
      541 aattccctaa caaacagagg tataaaattg ttgggagtat tccttaccat ttaagcacac
      601 aaattattaa aaaagtggtt tttgaaagcc atgcgtctga catctatctg attgttgaag
      661 aaggattcta caagcgtacc ttggatattc accgaacact agggttgctc ttgcacactc
      721 aagtctcgat tcagcaattg cttaagctgc cagcggaatg ctttcatcct aaaccaaaag
      781 taaacagtgt cttaataaaa cttacccgcc ataccacaga tgttccagat aaatattgga
      841 agctatatac gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta
      901 aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa caatttaagt accgttactt
      961 atgagcaagt attgtctatt tttaatagtt atctattatt taacgggagg aaataattct
     1021 atgagtcgct tttttaaatt tggaaagtta cacgttacta aagggaatgg agataaatta
     1081 ttagatatac tactgacagc ttccaagaag ctaaagaggt ccctagcgcc tacggggaat
     1141 ttgtatcgat aaggggtaca aattcccact aagcgctcgg gaccccttgt aggaaaatgt
     1201 cctaagtgtg gcaacaatat tgtattaaaa aaatcgtttt atggttgttc aaattatcct
     1261 gaatgtaagt ttactttagc tgaacatttt agaaagaaaa aactaaccaa aacgaatgta
     1321 aaagaattac tggagggaaa agaaaccctg gtaaaaggaa tcaaaaacaa agagaaaaag
     1381 ccctacaatg ccgttgtaaa aattggggaa aagggatata ttgattttat ctctttttca
     1441 aaataaacat ggggctgacc caaaagtagc tcctatatga aaaatagagg tctttccatt
     1501 tagatagtag tggaaagacc tctatttatc ttttgaaagt gaatacaaaa ataaagcaca
     1561 ctctgctata atataggtga ttaatccatt tgagagaggt gctttatcat gtatcaaaat
     1621 tataccataa accaactttg tttgcctata gatcttgaaa ttaaattaga agaaaatgat
     1681 tttgctcatg ccattgtgca gtttgttgac tctattcccg atgaagtctt tcttccttat
     1741 tatcaatcta tgggtagacc ccaatatcat cctcgtatgc tactttccat tattctttgt
     1801 gcctatattc aaggggttta ttcgggccgc caaatacaaa atatgctgat agatagtatt
     1861 cggatgcgct acttatctca agagcaattc cctaactttc gtactattaa tcgttttcgc
     1921 gttcatccga ttatgaacga tattctagat cattcatttg ttcaatttcg tgagctactg
     1981 gttcaaagtg gcttaatcag tggcagtgct ctatacattg atggtacaaa aattgaagcg
     2041 gatgcgaata aatactcttt cgtttggaaa aaaagtattt taaagtataa ggatagcctt
     2101 gatcaaaaag cccttgaaaa ctaccaacaa atggtggaaa caaaaatctt acctgagtta
     2161 atagatgatt taaaggatga actttctata gaagagattg aaaggattcg tcaatctttg
     2221 gaagaaaagg aaaatcaatt aattgaagcg attgaccaaa ccgaaacagt agaagggggc
     2281 tgacccatta ttaataagga gtggacaaca tgattgatgt aaatgagcta ttggaagaag
     2341 ctattagaga aactgaaaat ctaaatgatg gcgaagtttt tcttgttaaa gatttattca
     2401 agggatatgt ggggaatagg atacccagaa aggatcgact tctgcttgga acgttatttt
     2461 taaaccatgt tagcaaaatg aatggtaatt taaaggctat tgaaaagact tcatctaatc
     2521 agcagaggta taaaaagaca attgataagt tttaactatc ttttgtgaaa ttaaaaaaat
     2581 tataaatatg ttgagtagaa gcaaaagatt tggcttggaa tttttacagc agtgaatcga
     2641 taaaaggagc atgcttgatg gtagataata ttattaaatc agtagcagag aaattatcct
     2701 ctctgtctta tatagaaggt attgttttag gtggttcacg tgcaaggggc acccatacag
     2761 aggattcgga tatagatatc ggcatctatt acaattcaga atcatttgac ataaatacta
     2821 ttaatcaatt cgctacaaag ctggatgatg agcatagaaa taaccttgtt gtacctcccg
     2881 gagcatgggg tgattggatt aatggcggcg gatggttagt cataaacggg tatcatgtgg
     2941 atttaatttt acgtgatatt aaacgtgtgg aacaaataat gaaagataca gagcacggaa
     3001 ttgttactgc caattatcag actgggcatc cccatggtta tattagtgca atgtatcgag
     3061 gagaattagc gattagcaaa atactatatg ctaagaatga aagcttatgc gaattaaaaa
     3121 aacaggcaga aacttatccc aatgctttgc agaaaagttt agttaacttt tttatgtttg
     3181 aagcagggtt ctctttaatg tttgtaaaag caaattcggg aacagacgat aaatattata
     3241 ttgcgggtca tgtttttcgt atagtttcat gtttaaatca agtgttattt gcatgtaata
     3301 atgcttattg tatcaacgaa aagaaagcta taaaactgct tgaaactttt gaacataaac
     3361 ctgaaaaata taccgagaag gtaaatcata tttttgaagt actcggtatc tcactttttg
     3421 aatgctacga catgaccgag aagctttata atgaagtgaa tgaaattgta tcggagataa
     3481 ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga taatatattt
     3541 tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc gggagaatgg
     3601 gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga tttaggatgc
     3661 ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt agtaggtgtt
     3721 gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc acagattgaa
     3781 tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga tgtaatacta
     3841 agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa gatatatagg
     3901 atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt tactgctcat
     3961 ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc ggtggacaat
     4021 tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac aaaatatcat
     4081 agaacactga ccacatatct aaatacactg ctttcaaata gttttataat aaatcagatt
     4141 gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga tgaaatgcga
     4201 cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag aaaaaataaa
     4261 cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt tctttagcag
     4321 aacaggatga acgaacgtat tcgaattgtg acccttgagg ggtcacgcgc aaatattaat
     4381 atacctaaag atgaatttca ggattatgat attacatatt ttgtaagtga tatagaaccg
     4441 tttatatcta atgatgactg gcttaatcaa tttgggaata taataatgat gcaaaagccg
     4501 gaggatatgg aattattccc acctgaagaa aagggatttt cctatcttat gctatttgat
     4561 gattacaata aaattgatct taccttattg cccttggaag agttagataa ttacctaaag
     4621 ggcgataaat taataaaggt tctaattgat aaagattgta gaattaaaag ggacatagtt
     4681 ccgactgata tagattatca tgtaagaaag ccaagcgcaa gggagtatga tgattgctgc
     4741 aatgaatttt ggaatgtaac accttatgtt attaaaggat tgtgccgtaa ggaaatttta
     4801 tttgctattg atcattttaa tcagattgtt cgccatgagc tgctgagaat gatatcatgg
     4861 aaggtcggca tcgaaacagg ctttaaatta agtgtaggca agaactataa gtttattgaa
     4921 aggtatatat ccgaggattt gtgggagaaa cttttgtcca cctaccggat ggattcctat
     4981 gaaaacatat gggaagcatt atttctatgc catcaattgt tcagggcggt atccggtgag
     5041 gtggcggaaa ggcttcatta tgcctatccg gagtatgata ggaatataac aaaatatacc
     5101 agggacatgt ataaaaaata cactggtaaa accggctgcc tggatagcac atatgccgct
     5161 gatatagaag agaggcggga acagtgatta cagaaatgaa agcagggcac ctgaaagata
     5221 tcgataaacc cagcgaacca tttgaggtga taggtaagat tataccgagg tatgaaaacg
     5281 agaattggac ctttacagaa ttactctatg aagcgccata tttaaaaagc taccaagacg
     5341 aagaggatga agaggatgag gaggcagatt gccttgaata tattgacaat actgataaga
     5401 taatatatct ttactaccaa gacgataaat gcgtcggaaa agttaaactg cgaaaaaatt
     5461 ggaaccggta cgcttatata gaagatatcg ccgtatgtaa ggatttcagg gggcaaggca
     5521 taggcagcgc gcttatcaat atatctatag aatgggcaaa gcataaaaac ttgcatggac
     5581 taatgcttga aacccaggac aataacctta tagcttgtaa attctatcat aattgtggtt
     5641 tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaacaac tttgaaaaag
     5701 ctgttttctg gtatttaagg ttttagaatg caaggaacag tgaattggag ttcgtcttgt
     5761 tataattagc ttcttggggt atctttaaat actgtagaaa agaggaagga aataataaat
     5821 ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa
     5881 agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa atgaaaacct
     5941 atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg aacgggaaaa
     6001 ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc actttgaacg
     6061 gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt gctcggaaga
     6121 gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt gcatcaggct
     6181 ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca gccgcttagc
     6241 cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa actgggaaga
     6301 agacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga
     6361 agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg tgaaagatgg
     6421 caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt ggtatgacat
     6481 tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg tcgagctatt
     6541 ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata ttttactgga
     6601 tgaattgttt tagtacctag atttagatgt ctaaaaagct ttaactacaa gctttttaga
     6661 catctaatct tttctgaagt acatccgcaa ctgtccatac tctgatgttt tatatctttt
     6721 ctaaaagttc gctagataga gttctatatt agagatgtaa agagttttgg tgaagaggtt
     6781 tgtgagtatg gcttttataa ccagggtgca atgagaagca ctaacagcag ctagcttaag
     6841 aacgcatagg atatactttt tatggaagtc acagaggagg aataaaattt gaacaggatt
     6901 aacagagtaa cctctattct tattcagctg caatcaaaaa aaataattcc tgccaaagaa
     6961 attgcacagc gatttaatat aagcttaagg acagtttaca gagatatccg gacacttgaa
     7021 gaagccggaa taccaattgg atctgaggcc ggaaaaggat attttcttgt agagggcttc
     7081 ctacttccgc cggtaatgtt tacagcggcg gaagtgggtg cattgattac agcagggaaa
     7141 tttttaaatt gccatggaga tgaatcattt ataaaggatt ttgattcagc tatgtataaa
     7201 atcaaatcta ttttgaagca tggtgaaaaa aactatgcac aggagttgga gaacagtatt
     7261 aacgtgtaca gcacatctgg acagaaaaat acattggcgg ataacgttat tgcagcaatt
     7321 cagactgcaa tctgcaataa aagggtaata tcaattcaat atcctgcatc aggagggcaa
     7381 gaaccggaaa gcagaatgat aga
//
LOCUS       DACEPD010000108         7046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.114, whole genome shotgun sequence.
ACCESSION   DACEPD010000108 DACEPD010000000
VERSION     DACEPD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.114"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..104
                     /locus_tag="IW797_002423"
     CDS             <1..104
                     /locus_tag="IW797_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ1225177.1"
                     /translation="FQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(172..465)
                     /locus_tag="IW797_002424"
     CDS             complement(172..465)
                     /locus_tag="IW797_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ1225178.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(493..1158)
                     /locus_tag="IW797_002425"
     CDS             complement(493..1158)
                     /locus_tag="IW797_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ1225179.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1298..1930)
                     /locus_tag="IW797_002426"
     CDS             complement(1298..1930)
                     /locus_tag="IW797_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1225180.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1937..3004)
                     /locus_tag="IW797_002427"
     CDS             complement(1937..3004)
                     /locus_tag="IW797_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ1225181.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(3001..3732)
                     /locus_tag="IW797_002428"
     CDS             complement(3001..3732)
                     /locus_tag="IW797_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ1225182.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(3900..4379)
                     /gene="greA"
                     /locus_tag="IW797_002429"
     CDS             complement(3900..4379)
                     /gene="greA"
                     /locus_tag="IW797_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ1225183.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4515..5690)
                     /gene="mltG"
                     /locus_tag="IW797_002430"
     CDS             complement(4515..5690)
                     /gene="mltG"
                     /locus_tag="IW797_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ1225184.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5855..7015
                     /locus_tag="IW797_002431"
     CDS             5855..7015
                     /locus_tag="IW797_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ1225185.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
BASE COUNT         2086 a         1486 c         1105 g         2369 t
ORIGIN      
        1 attttcaaaa gaaattaatg gctatttttt taaccaatct gaaaagctac tttgccaccc
       61 atgagcttca aaccaatcaa gtaaaattgt ctgcaggata ttaatagctg aaaacaatat
      121 tttcaaaaga aaaaagagaa ggtgacaaaa attgtgtcac cctctctttt tttattgttt
      181 tttaaagtga taggttggct cctccaggtt gggatcataa tcaacaagta aatcatatcc
      241 tttaaagaac cattcatctg cttcctcgat aaaaaaaagt gtcccatcca tttccacttt
      301 ctttacagga tgttctggct gttcaactga cattccaatc gaaaaaccat catgaacctc
      361 tgttttgccg tagacttttc caaaaaaacg tattccgtaa gtttctggca gatcaatttc
      421 tgagtcaaac cacttttgtg cttttggcgt taccgttaat ttcatcccaa caacctactt
      481 tctcttttct ttctattgat tcatcttatc gtctagaata tccacatcgc ctgtctctcc
      541 aataaccagt agattatcat tttcacgaac atattcatct gctgcaggag aagcgataac
      601 cgtttgatct actcttcgta tggcgactac tgttaagtca aaacgttgcc tgaaatttaa
      661 ttcagctaat gttttgttga aaaatttagg attggagact cggatttctg ctaaagaata
      721 tttatctgac agctctaagt agtctagaat attctttgat actaagttat gtgcaatccg
      781 tatccccatg tctcgctcag ggtgtacgac acgatctgct ccgatcttct ctagtacacg
      841 ggcatggtac tcattttggg cttttgctaa aatattcggt acgcccattt cctttaccat
      901 cagagtaact aagatgctgg cttggatatc ttctccaata gcgacaatta catgatcgaa
      961 atttcttata cctaatgaac gaagtgtcat ctcatcctgt gcatttgcca caacagcatg
     1021 ggtggctata ttcatatatt cattgactcg atcttcatta ctgtcaatag ccagtacttc
     1081 ttgtccagct tcgatcagtg tacggcagat gcttccgcca aatcgtccaa ggcctacaat
     1141 tgcaaaattt tgtttcacca ttttcctcct ttagtatctt cttttctcag tataacaaag
     1201 cctttccaaa aacaaaagca agcatcatta ttgtaaaaaa gccaagaaaa ataatagtta
     1261 ttttctcagc ttttgattat cctctttatc atcgttttta tttcaccaaa ccatgtttaa
     1321 acgcatagat agctgcttga gttcggtctt ctacctctaa cttagctaat atattcgaca
     1381 catgcgtttt gactgttttc agtgtgataa acaattcatc cgcgatttcc tgattacttt
     1441 tcccttcaga aatcagcatc aaaatttcat tttctcgatt cgttaattct tcatgcaata
     1501 ctggatcatt tcgccgactc attttttcca tcatttttgt tgtcacttca ggttctagta
     1561 ctcgctcgcc gcgttgcgta gcccttatag catcagctat ttcatgtgct gtagacgtct
     1621 tcaacaaata tcctgctgct cctgcttcaa ttgcaggata aactttttca tcatcaataa
     1681 aacttgtgac gataatgatt ttggcttttg gccaatcttt taagattgct tttgttgatt
     1741 cgatgccatc catttcttcc atgactagat ccattaaaat gacatccggt cgaagtgcca
     1801 tcgccttttc atagccttga cgtccatttt cagcttctcc aatcacttca atgtcttctt
     1861 ggatagataa ataagaagag acgcctaagc gtaccatttc atggtcatct actaataaaa
     1921 cttttatcat ctttatctat gcctccttca tcaaagggac cttggtctcc acgctcgtac
     1981 cttgcccttt aaaactaatg atttttactg ttccaccaat accagctact cgttctttga
     2041 tattgttcaa tccataactt cccgttttgg tttcattcat attaaatcct gtcccgtcgt
     2101 caatgatacg taataaaaga ttgttatcta ttttatgcaa atagacttct aattcattag
     2161 ccttagcatg tcttaatgtg tttgataaca attcttgaac gattcggaac aagtgatcct
     2221 caatggaact agttaatttt acatcttcta cctcccattt tagcgaaatc tggattttat
     2281 tctgcaattc cttcaataac tgctcaatgc cttgttttaa acttttttct tctaaattga
     2341 ctggacgcag atgaagaagc agcgcacgca tctcagactg ggaagcgttg atgatctctg
     2401 ccaccatttt caactgctta cggaacattt ctggtgtctc acttttttct gcttgctccg
     2461 tcaatgccga catcatcatc atcgctgcaa ataattgctg tgaaactgaa tcatgaagct
     2521 cacgagctaa tcgatgcctt tctaatttta aaatctcttc tttggtttgt ccatctacat
     2581 attgaggacg gctagtcata atctgtaatt cactggatac ttcgatcatt ttttctttta
     2641 ttttagtaat ttccatatca atatctttga tgtacagatc atccgacgcg cttgggatcg
     2701 gctgatgcaa aagtttggat tcataattgc cagaagaaag tgcacgaagc ttttcttcga
     2761 ttttcccgta ttttgccttt tgaataagtg ataacaatag aaatacgatc aatcctacac
     2821 ctaatgaaat tgccatcaaa tggaagataa gcggaacgta taataaacgc gcacgcagca
     2881 attctcccca ccaatgactt tggttactgg cataaaaata agtgaaaagt gaaaatagaa
     2941 taataaggaa agccgcaatc cccgaataaa cagctagcat tgctttggat attcttccca
     3001 tcatattcgt atcacctcga catctccaac caaagtattg gtaatgatct tcaatctgcg
     3061 aggattttca tcataatcac tgctgcagat tttgatctgt tcgtttttta aggaaaactg
     3121 ctcttcttca aacaagacat ttcctactaa tgtcgcatgc tctaaacgaa tagccactcc
     3181 caggggtact aagatccgtg ttctgcctat cccttttctt acaatcacaa tattatcatc
     3241 ttttggcaaa agtgtattgc ccaaatcgat gatagtatca ccagaaatga tggcaatgtt
     3301 gatatcatcc cattcataga cgtcgtttcc aatacgttgg ttaccaaata attgctgtct
     3361 tttacgctca ttattatgtg tttttaattg ctctgtttgg accatcatga tctgtttttt
     3421 tcgccaaaaa gcattttttg tcaaatctat tcctgaaatc tctacacctt tcagcccgat
     3481 aaataaaacg gccaatacaa ccatcatcca taaagcagga ctgttgacta agctaaaaaa
     3541 aatgaccaag ctccctaaga tcaattgaaa attttgaaat ttagtacgag gatatttgcg
     3601 catagctaga tatatattga aaataccgaa tattactaaa agcaaaagtc cagtattgtt
     3661 tacgatttgc caaacagcaa ataacagcag cagtgcttcg actaccacaa aaaaacgcca
     3721 agaactattc atcgcttttt ctccctcctg acacattctc atttcatcat acctattttt
     3781 ttgactgttg gctataggac tttagatgta agatcgttca gtgaaacctg caatactcaa
     3841 agagaaaaga tctagagtgc gtcatcagta agatgaactc tctagatctt tcagtcagac
     3901 tacgcttgtt ctactttgac gattttaact aacatatcgc cacctggtgt agcaatggtt
     3961 acttcatcat ttttacgttt gccaatcaat gcttgcgcga taggagaatc attggaaatc
     4021 tttccagaaa acgggtctgc ttcagcactt ccgacaatcg tgtactcttc ttcatctcca
     4081 tcaggcaact cgatgaacgt aactgtttta ccgatcgata cctcatctgc atctacgccg
     4141 tcattatcga tgatttgtgc aaaacggatc atattttcaa ttgttgtgat tcttccttca
     4201 acaaatgctt gttcatcttt agctgattcg tattcggagt tttcggaaag atctccgaaa
     4261 ccgcgagcga ttttgattcg ttcaatgatt tctccgcgtt taactgtttt tagttcttct
     4321 aattcttttt ctaatttttc tttaccttct agagtcattg gataaacttt ctctaccatg
     4381 tgtaacgtct ccttttttcg ataaaatacc gcaaaaggta atcttttgag gaatatagtc
     4441 caaaaaagat taccacgctt ttcttaaaat gtaaatgttt ttctaaaaat gatcaatgac
     4501 tagcccctgt ttttctaaga attatttaca tatttttgca ctagttccat atgttcttca
     4561 tacgttttag cgaaataaac atttccagtg ctcgtatctg ctacgaaata ataatagtca
     4621 ttttctgctg gttctaatac tgccttgatt gcctcttcgc ttggattgtc aaaaggacca
     4681 ggcccatagc cggtattggt atacaaattg tatggtgaat ctacagctgt atcttcatac
     4741 gtcaccaatt ctttatgttc tcctaatgca tataaaacag agatatctga ctgaagtggc
     4801 atatttgcct tgattcggtt aaagaagact tgtgcaatat ttttacgatc attttctttt
     4861 acgccttctt tttcaactag agaagccaaa gttaaaactt cttgtacagt caaatttttc
     4921 tgtttgatag ctgaataata gttttgcatg accgtatttg ttttgttcac catctcaatc
     4981 acaagatcct taagcgaagt actcgtgtag taatcataag tagctgggaa taagtagcct
     5041 tccaaacggt attttgtgtt ctcagcttta gaggcacttt ccaacaattc tgggaatttt
     5101 tgatacaact cgttaaagaa agtctcatca tttactaaat ctaaaaattc tttcttgtct
     5161 ttaccagtta ccttcgctac ccgctcagca atctgatcga tgtcgtagcc ttctggaatg
     5221 gcaattttcc catctgcaac ttttgttggc tcagatgtgc cgccttcttg caactgcttg
     5281 ccaatctcat caagagtcat attaggcgct aactgataat aaccagcttg aaagccagtt
     5341 aagtttttaa attttgtata ataattgaaa acaatcccac ttttaataat attgtctttc
     5401 tccaaaattt cgccaatctg cttattagaa gagccgcttg ggatttccac ttggactaat
     5461 tggtcgtccg atttatctaa aggcttcaac ccgctatcta cataacgata tacggtaaac
     5521 ccgagaaatc cgccaataat caataatgta agagcgatca ctaaaatgat cttcctcacg
     5581 atcttatctt cttttttacg cagatttctc cgcttgtcga ttatatctgc tgtagctgcc
     5641 ttactggctt cttttttgtc tttgcttggg tgttgtttgt ttcctgacaa aacaactcct
     5701 ccttactatc atcttcgtaa ctctgtttat tatacatgaa atttctactg ccgtgaatta
     5761 ttttaccaaa tattatgtca aaattgtaag tggcaaatgc tctttttgag gattacaatg
     5821 aaaaatagtt aaaaatggat aggaaggatt taatatggca aaaggaaaac atcaaaaaaa
     5881 acagcagatc tctctttctt ttctcgcagt tttgttgctg gctgtcagct tcttttcaac
     5941 aggcatcatt acaggatggc aattaacaaa acaacctcaa ccacaaattt ataaaacgga
     6001 acccgcacaa cttgcttcca atttaacatc taatgagcag atgcttgcca gcaaaattga
     6061 tcaattactg caaaaatcag attatatcgg ttcgatttat gtacgcaaaa ataatcgagt
     6121 gatattagaa aaaggatatg ggtacgcaaa tctacgcata aaagaatcga attctccttc
     6181 tctttactat caaatcggtt cgatccaaaa agcgatgact gctttactta tcctaaaaca
     6241 aattcaatct ggcaagataa cttttgacac aaagctatct gaattttatc ctcagattcc
     6301 tggaagtcag caaatctcaa tcaaaaatat gctttacatg cgctctggtc ttcatcgaac
     6361 cgcttctcca aaaaaaccta tgtcagatag tgagatcatt cagtttgccc ttcatcattt
     6421 gaaatttact ggttatgata cgtatcatta tgaaccatta aattatacat tgcttacagg
     6481 ggtattgatc aaactaatga atcagcctta tgaaactctt ttaaaaaaag aaatcattcg
     6541 gccactccat ctagaacata cagattttta tgaaaatgta aaaaattccc ctcagcatgc
     6601 tgtatcttac caaatgtctg caactgacga ttattcaaaa gcattggtcg agcctgaaac
     6661 agacatccga aatgaattag gcacaggaaa tataagtatg tctgtttatg acttgaacaa
     6721 attctttacc agtgttctgt ctggcgatat tataccaaaa gaactacttt tctcattgtg
     6781 gcaaaataat ggtgataaac atccttacag cggcggtgtt tacagtggaa atgattatat
     6841 ccttgctcag ggaaatatca atcgattcca tgcagtagcg gcgatgaaaa aagacacaaa
     6901 agatgcaatc gtcatggaaa gtaatatcca atctgataaa aacatcaaat tgccggccac
     6961 tgatttaaga aatcagattt acgaactgat ggaaggtgtt aatttaaaaa gctaagtcga
     7021 gacaaaaaaa ccagcaggat gctggt
//
LOCUS       DACEPD010000109         7038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.115, whole genome shotgun sequence.
ACCESSION   DACEPD010000109 DACEPD010000000
VERSION     DACEPD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.115"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            685..879
                     /locus_tag="IW797_002432"
     CDS             685..879
                     /locus_tag="IW797_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225186.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            1070..1319
                     /locus_tag="IW797_002433"
                     /pseudo
     CDS             1070..1319
                     /locus_tag="IW797_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1563..2393)
                     /locus_tag="IW797_002434"
     CDS             complement(1563..2393)
                     /locus_tag="IW797_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ1225187.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2601..3935
                     /locus_tag="IW797_002435"
     CDS             2601..3935
                     /locus_tag="IW797_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ1225188.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3976..4887
                     /locus_tag="IW797_002436"
     CDS             3976..4887
                     /locus_tag="IW797_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ1225189.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4884..5738
                     /locus_tag="IW797_002437"
     CDS             4884..5738
                     /locus_tag="IW797_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ1225190.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIILLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            5735..6352
                     /locus_tag="IW797_002438"
     CDS             5735..6352
                     /locus_tag="IW797_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ1225191.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            6354..6872
                     /locus_tag="IW797_002439"
     CDS             6354..6872
                     /locus_tag="IW797_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ1225192.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2117 a         1205 c         1401 g         2315 t
ORIGIN      
        1 aaaaattcta aaggattcct aaccatctta ttagctttta tagggtatat gttggttggg
       61 ctgctaaaat catacagtaa tgagttgctg aatttttcaa catttattaa tgatacatta
      121 gttccaagtt tgtttttcat tgtatttttt gctgttggtt attttataat aaagatatga
      181 tgactaattt agctcaacta ttccagcgtc agctgaagat attgcaattc ccgattcctt
      241 aatcatttat ctggatgaga cagaaagtga agttgcaaca gatgtgcatg tagttgagga
      301 taaaaatgat agtttaaaaa ttaatagttc agatacttta ctaagagtaa gtcctatctt
      361 ttcagctgtt atttagatgc aagtagcaac taagttaggt agtactcctg caatggatag
      421 gtatttgtgt gttcgatata taagtggtcg aagtatcaag gttatgtata ctggatggga
      481 gatagaagag ttaaaaattg gaggtcaccg ccacaaatgg aatttagatt tagaggtacc
      541 ttagatttgg tatagttatt aaacacaaga tggcctggtt tgtagggaaa gtcgaagagt
      601 atttttccac ttctgcgccc ctttaatttt tctagtaatg ccgtgaattt gtgattaatt
      661 ggaaatatga agaaaggaga gtctatgaaa acaacactag ttctattcta taaaaagcat
      721 ccttatttta cacttctgat aaatatattg ttggctagtg ttatcgggat ttctgttgaa
      781 tatctaataa ataaagattt cataggttct tgtttttata cggctttatt tcttggactt
      841 cttgaagcat tttccattta taaaaaatca aaaaaataaa atagattgcc gtaagagatc
      901 tttttcattt catttttttc tgaaaacccg ttgtttctgt cagtgaagca gcgggttttt
      961 agatttattg tctttagacc taattgagcg cgccgagcga ttgaaataca aaaccacttg
     1021 ctatgcgggt ggagcaataa agggaaatag tttagcccgt acaaagtaga tgtgtaaata
     1081 ccacattgta ttcacaccaa agtatagaag aaaaataatt tataatcaat atcgagccaa
     1141 tattcaggag atcatcaaga gattgtgcaa atctaaaata taaatttggg tatcgacact
     1201 tttgggtgga aggatactat gtgacaaaag taggacaaaa tgaagcgacc atcaaaaagt
     1261 acattcaaga acaagaaaaa catgatagtg cgttagataa gctgagtgta cgagagtaag
     1321 acgatccttt taagggctaa ggagtagtac ataaaccttt taagaggtgg gcaaaactgg
     1381 caaaggcaat atggcttgaa cataaaatat gaaagccagc gtctttaggc gctggccggt
     1441 gatttgagct tatagctcta gagaaaacca cccttttgaa gggtggttat gattaatgac
     1501 tttagtcagt tttgctcaca aagcgtgtgg aacaaaaaac gggtgtttct acgggtagat
     1561 atttatttct tgaatggagg tgtcccatac atttttggtg ttactggttc taattttggt
     1621 gcagcgcctt cgatcttagg ttctttcaac cattgatatt cgccttcgcc atccagtgct
     1681 ttgccactag cccaagaacc ttcactactt tggtctccct ctgagaagtt atagaagtca
     1741 taagcaatgt cttttcgttc tttgtcacgc gggaaagtac ttgggactaa gattgaacct
     1801 tctttttctt ctaattcttt cactgcacat tcccatagat tttgatgata gctatctctt
     1861 gcaaggagaa cagacagcat atcttttacg ccacgatcat cgatcatttc gtaaatacgt
     1921 gctacttgaa gtcttccttg tgattcggca tttaaattag ctctaaagtc tgctaacaag
     1981 ttgccactcg caatgatata ggcaccgctc catggattgc ccacgctgtc agcaggacga
     2041 ggtcctaagc cagctacgat cgcgtgctgt ggattcattc cagagataat ggcaccgatt
     2101 gttggatctt tttgcatcgc tgcttcttga tcttcaacag gcgcaccctc taataaacga
     2161 gcaaccattg tcgccaacat ttccacgtgg ccgatttctt ctgttgcaat gtccatcagc
     2221 atatctttat atttttcttc tcctcggcag ctccagcctt ggaacaggta ctgcatagct
     2281 acactcatct cgccatattg gccgccgatc agctcctgca gatatttcgc gattaatgga
     2341 ttcggacgat ctggttttgc ttcaaactgc aattctttct ggtgtctaaa catcaaaatt
     2401 cctcctaatc tatttttaat acattctcat tctgctacga aagattaaaa aattcaaaga
     2461 aagcgctcgc gaattatata aagtattggt atcattaata atttttttag aatcatacct
     2521 tataaattgt tattttcaga acaaaatgat atcgttatat taaaaccggc atcggttaca
     2581 ttaaaatttg gaggtaaaat atgaaaaaat tattgtttac agggttgctg gcaagttcag
     2641 cattgttttt attaggagga tgcggatcaa gtaatgcaag cgatgccagt tcaagtgcag
     2701 caaattcttc tgaaaaaaca agtgatggaa gaaaaaatat tgatttctgg tccttttggg
     2761 gatccggtgc acgaaaagaa gctatcgaag aaatcattga ggatttcaat caatcacaaa
     2821 acgagatcca tgtaaattat aagtatcagc catggggtga catctggact aaatctttat
     2881 ctgcaataac agctggaaat gcacccgatg ttattgtaca ggacataaat tccgttgccc
     2941 aaagagcaga agcacagcaa gcaactaatc tctccaaata tgtagaaaaa gacacttcta
     3001 aggattttta tccgcagctt tgggatacag tagaatatga aggtgatcct tatgcgatcc
     3061 cattcaatac agatacacaa gtcatttttt ataataaaaa gttatttaaa gaagctggaa
     3121 tcagtgaaca agatctaccg acaacttggg atgaattaga agaaacagca cgtaaactag
     3181 ataaaaaaga aggaaaagaa tttacacgca tcggttttta tccattatgg aatttagggg
     3241 ctgatgtatg ggcgctaaac gcagataacg ggactagttg gtttgacaaa gatgaaaaag
     3301 tcaagattga tactaaaaat aaagtggaag ccttggagtg gatccaagac tggcaagatt
     3361 attatgggaa aaatacgatc aataaattag aagccgaatt tgggtctggt gtatctgatc
     3421 catttatttc cggattagta gccatgcgtg cccaaaatat caattattac tctagtttga
     3481 tggaaaatgc accggatgat tttgattttg gcgtgatgca gctcccagaa aaagaagaag
     3541 gttcaggcca ttggtcgtgg ggtggcggat tcgtcttgga agtaccatac ggagcaaaag
     3601 atcctgaggc ctcttatgaa ttcatcaaat atctttcttc tccagaagta caagaaaaat
     3661 tcggcgaaaa aagttttgat atcatggcga gcaagacagc caatgagaat ttagtgaata
     3721 atgacaacct aacggataaa ggaaaaatga tctatcaaat ggcggacgaa aactttaaaa
     3781 atacggtgat cacaccagtt cctttagatg ctccggatta cacgacatta gtgaatgaac
     3841 agattgacca gattctttta ggaacaaaat caccaaaaga aggattggca gatgcacaaa
     3901 aagcagtaga aaacttagtc aagcaaaatc aataatccat ttttgactgt ctaggtacag
     3961 aggaggcaag aaaatttgaa gcgtttatct atccgaaaga aaaaagaagc aagatgggcc
     4021 tatttattta tctcgccttt tattatcggg ttcttggtat ttatgctagg gcctatgctt
     4081 ttttcagtta ttggtagttt caccgattat aatttgacat ccaagatgaa tttcattggt
     4141 ctagcaaatt tcaaaagaat gttcgttcac gatgatttat tttggaaatc gttatacaac
     4201 accgtttatt tcgtcatctt caatgtaccg ctgacgacga ttttcagtgt gtttttagcc
     4261 acagtactca atcaaaaaat caaaggaatc agtgtattcc gtacagcttt ctatttaccg
     4321 gcagtcttat caggagtagc agtctacatc ttatggatgc agctgctttc tccatctgca
     4381 ggattgatca acacatttct gggctggttc catatccctg gaccagcttg gctgtttgat
     4441 ccacaatgga cgaaacctgc gttgattttg atgaaaatat ggagttctgg aggagcaatg
     4501 ctactttact tagctacatt acaaaatgtt cctagatctt tatatgaatc agcagaaatc
     4561 gatggagcag gcgtttgggg aaagttcaaa agtattactt tgccattaat cactcctatt
     4621 attttttacg atgtgatcac ctctacgatc ggttctttcc aaatttttca agaagcttat
     4681 gtcatgacga aaaatggaac aggcggacct gcaaattctt tgctttttta taatctgcat
     4741 atgtggaaca aggcgttcgt agcttttgat atgggttatg cgatggctat gtcaatgatc
     4801 ctgtttctga tcgtgttggt tctgacgttt gtcaatttga agcttgcacc taagtgggtc
     4861 tattattctg gaggtgacgg caaatgatgg attatttcaa aaagaccagt gtgaatcggc
     4921 aaacaaaaaa aatactaagt tatgtcctca tgacagtaat aggaatcatt ttgatcattc
     4981 tattgctttg gatggtgttt acttcattga aaccaatgga agaaatcgtt cgttatcctc
     5041 cgaccttttt cccagaaaaa atcgtatggg aaaactatct ggatacgatt gccgcctttc
     5101 ctttttggcg ctatgcaaga aatacattat tcatcactgt gctagtagtg atcggtaatg
     5161 tcctttcaaa ttcttttatt gcttatggat ttgcgaagct tgattttcct gggaaaaagc
     5221 tgatgtttgc tttggttctt tctacgatga tgatcccggg gtttgtaacg atgatccctc
     5281 aatatgtatt attctccaag atcggctggg taggtactta tttgcccctg atcgttccat
     5341 cgttcttcgg caatgcgttc aatatatttc tgatgcgtca attttactta tcgatcaata
     5401 atgaattgat cgaagctgct gaaatcgacg gagcgaatca tttgtatatt tggagtcatt
     5461 tgatgcttcc attgacgaaa cctgccttga ttaccattgc gatcaattcc ttcaacgcag
     5521 cttggaatga tttcttaggt cctctgttat atatccaaga tcaagaaaaa tacacgttac
     5581 agattggctt gcaagttttt cagaaccaag caacgacaca atggaattat ctgatggcag
     5641 gagcaacact tgtcttgatc ccgacgattt tgctgttctt ttttgcacaa cgttatttca
     5701 ttgaagggat ggatttgaca ggaggttcaa aaggatgatg aatcgatcga ttggtaaaaa
     5761 agccaatgat tatttcttag aaatcattga atggattcca cgtcttatcc atttacagct
     5821 actatggttc ctgtgcgtct tgcctgtttt cacattagga actgcaagtc gaacagttct
     5881 gtatatgatt tatcattaca aaaaaaatga agagaaaaag ctcggttcac tattctggaa
     5941 aaagtttcgt gagaacttct ctctttatgg aaagcaagat agtttagcaa gtgtttattt
     6001 tcttcttcta ttgatcgact atcagatttt ccgctattgg ggtgggggaa ttggctatac
     6061 attgatgtat actacgttgt ttttgcttct attaagcgcg cttctcttta gttacaaaat
     6121 cttgcttcaa ctggaacaaa aagaagcttc ttggattact gcatttttct tgttttttaa
     6181 tgatttcaaa agcgcgcttt tctatttggc aggcacgttg atccttcttg tcgcattctg
     6241 gtttgcagga ccaatctatt ttgctttatt aggtttcagt ttcttgtttt attttcaagt
     6301 cctgcttttt atccatcgta cgcaagaaaa ggttcttaaa aaggaggaat aacatgttgc
     6361 gtttgattgc taaaatcgat gatagtatta tcaacaagtt cgccaagcaa cggaaaacgg
     6421 atagtcgaaa aatcaatttt ttgaaattta tcagtaatat ctctaatggt ggttttattt
     6481 ggttgctcct tgctgctgtt ttagtcttga tacctgatac tagagaatat ggtctagcca
     6541 ttcttatcgg cagtttgatc tatttttttg ttggtttgct ttttatcaaa aaaacgacaa
     6601 gacggaaaag accttatgaa aaaaatcgtc ggcttgtcct ttacacaagt aaaccgaaga
     6661 cttcttcttt tccttcagga catacagcgc tagccttttt atcagtagct gcgttgttcg
     6721 aattaagtcc agttgcagga atattagcct ttttatttgc ttgtttgatc gcttattcga
     6781 ggattttttt acttttacat tatcctactg acattttagg aggagctatt ttaggaagtg
     6841 ttattggcat cctatgcgct gccgttatct gaattaattg tacgaataaa gcatgagtgc
     6901 gtaaatgcgc atttgtgctt tatttttttg tttcgaggaa agcgtagttg tttattcatt
     6961 tgtcttaata acttaagttt tgttaaatga gaacgttata aaattttata atttaatttt
     7021 aataataaat gttgtata
//
LOCUS       DACEPD010000110         7006 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.116, whole genome shotgun sequence.
ACCESSION   DACEPD010000110 DACEPD010000000
VERSION     DACEPD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.116"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            318..914
                     /locus_tag="IW797_002440"
     CDS             318..914
                     /locus_tag="IW797_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1225193.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(972..1454)
                     /locus_tag="IW797_002441"
     CDS             complement(972..1454)
                     /locus_tag="IW797_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225194.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1560..1757)
                     /locus_tag="IW797_002442"
     CDS             complement(1560..1757)
                     /locus_tag="IW797_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225195.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2052..2810
                     /locus_tag="IW797_002443"
     CDS             2052..2810
                     /locus_tag="IW797_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ1225196.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2870..3298
                     /locus_tag="IW797_002444"
     CDS             2870..3298
                     /locus_tag="IW797_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225197.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3344..3416
                     /locus_tag="IW797_002445"
     tRNA            3344..3416
                     /locus_tag="IW797_002445"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3377..3379,aa:Gln,seq:ctg)
     gene            complement(3566..3937)
                     /locus_tag="IW797_002446"
     CDS             complement(3566..3937)
                     /locus_tag="IW797_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ1225198.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4152..4337
                     /locus_tag="IW797_002447"
     CDS             4152..4337
                     /locus_tag="IW797_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225199.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4420..4608)
                     /locus_tag="IW797_002448"
     CDS             complement(4420..4608)
                     /locus_tag="IW797_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225200.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4632..4895)
                     /locus_tag="IW797_002449"
     CDS             complement(4632..4895)
                     /locus_tag="IW797_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225201.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            5053..5310
                     /locus_tag="IW797_002450"
     CDS             5053..5310
                     /locus_tag="IW797_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ1225202.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5381..5572)
                     /locus_tag="IW797_002451"
     CDS             complement(5381..5572)
                     /locus_tag="IW797_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225203.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5713..6033)
                     /locus_tag="IW797_002452"
     CDS             complement(5713..6033)
                     /locus_tag="IW797_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225204.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(6056..6490)
                     /locus_tag="IW797_002453"
     CDS             complement(6056..6490)
                     /locus_tag="IW797_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ1225205.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            6825..>7006
                     /locus_tag="IW797_002454"
     CDS             6825..>7006
                     /locus_tag="IW797_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ1225206.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2198 a         1363 c         1071 g         2374 t
ORIGIN      
        1 acttatttaa gaaatataaa ctaattttct ataaaagcaa gtaattttta atggcaatat
       61 cataaaaaaa agaagataga gatttaataa ttatatatca gctgtttttt tcaaataagc
      121 aaaaattcct aaaacacaac aatgaaagcg ataactaaga aaatccttac ataacgattt
      181 ttcttattaa aattgttttt tgaacttcgc ttattttttg tgagcatatc tgtttactta
      241 ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa aaaaatgcaa aacgcaaatc
      301 aaaggagaaa ttttattatg acaactttta ctgacacttt gaaaaaccgc cgttcaatct
      361 atcatctagg acgtaatgtt tcactttcta atgaagaatt aacaactttg atcaaagaag
      421 caattaaaga aagcccaact gctttcaatg ctcaatcaac acgcgcagtt atccttttcg
      481 gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc acttcgccca ttgactccag
      541 cagaagcttt cccaaataca caaaacaaat tagctggttt caaaaatggt tatggtacag
      601 tattattctt caaagatact gacgtggtaa aaggtctaca agaacaattt gaattatatg
      661 cagacaactt cccagattgg tcagaacaat caaacggaat cgcaacagct aatacttggg
      721 tagcattagt tgataaagga ttgggcgcaa acttgcaaca ttacaaccca gttattgacg
      781 aagcagttgc aaaagaatgg aatatccctt caaactggaa actacgttcg caattagtat
      841 tcggttcacc agaaacaccg gctggggaaa aagaatacat gaacgatgcc gatcgcttcc
      901 gcgtatttgg ataattttat ttgaaataaa aaacagaggt tgtgacaaac atcttgtcgc
      961 aacctctgtt tttattctgg atcaatcata atatcaatct ctgcctcatc gtttaagcgt
     1021 tgggacaaaa atggaactaa ttcttctaat gatgcagaaa atttcagcat cggtcctcgc
     1081 catccgtttc ggaaaaaatt catttttacg attttagaac cttttggctg atataataca
     1141 attttattga tctcagaaaa aggaacacct tttttgtcaa agctattcaa aacaagtcct
     1201 tcttctgtga ttcctctact gtccaacaaa aaactaataa aaatcattgc tgacaagatc
     1261 ccttttgcca attcatctgc tgtaggatga ctgatgagtg tataccaaat gactagtgac
     1321 actaagaagg gtatcaccaa gtagacccat cgatttcgca cagtccgata aataatttct
     1381 tttcgaacca gccaaagaaa taaaaacatc acaacaacga ataaaaacaa tagaaccgta
     1441 aagaatgtta ccatgacaac tcgcctcgct ttttcttctc cagaaaatag gaaaagaata
     1501 ctgatttacc ttctcaaaga aattggactt gcagcacgcc gcacgtccaa ttgttttctt
     1561 tattcaaatg tatcatattt tgcaacaacc atgcttgctt tttcatctaa taattcttgg
     1621 tcctctgcag cacattgggc aactttgacc acggcgcagt ttgtcatttt tgatacaact
     1681 tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg catattgttc tcctaaatta
     1741 atcatttctt ccgtcatcaa aatcctccta aactgctgga acttttctcc acattaccac
     1801 tgctatttct ttggagattt aactcattga tttttattta cagttaaaaa tcaaccttac
     1861 aggcattata gcgtactgtt ttcgttttca caattaaaac agctgttgta tttagaagaa
     1921 tttttttcaa aaaattaaaa catattcctt aaaaactaat tatttcctac atattaaaat
     1981 ataatacttc catttccaat aaaaatacat ttataatagg aaagataaac agctgaaaag
     2041 ggagatttca aatgaagaaa aaaacgttca aaaaactatt gtttctaggg ggcacagctg
     2101 ctcttttatt aagcggtaca agttattacc tacatatcca ttctccagaa catacaatga
     2161 acactgcaca aaaaagttca acacaaaaaa cgaaaaagac aaaagagaaa gcagaagttt
     2221 tactagatgt tccattagag agccagtttg attctccttc tttagaaaat ggctgtgaag
     2281 tcacttcgct ttctatgctt ttatcttttt atggttatga aacaactaaa aaccagttag
     2341 ctgagcaatt ggattacgtc cctgttttca atgcagatgg gactcatggc gatcctaatg
     2401 aaggatttgt tggtgatatc agtggcggag actgggctat ggtcgtctat gttcccccag
     2461 ttgcctccct tgctcagcaa atcgtccaaa cggattatca cacttttcca aaaaccgatg
     2521 ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa tcctgtctgg acttctgtga
     2581 ctatcaattt tgaagtccca gatgaaactg attttatgac ttggaccaca aataatggag
     2641 aagtaagagt cactcctctt gttcatgctt gtgtagtcac tggatatgac aaagaaaata
     2701 tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt accgatcaac gatttttcgg
     2761 cgattttcac tgcaatgggt ggacagatgc taactttgga gaaaagctag taattcaaac
     2821 aaaaatccct tatactcaag ataccaaacc tttgaaggga gcgtagataa tggaaaaatt
     2881 cacacataaa aaaatggatc caaatgaaat tccgattatt tttgtccgtg actgcaaggg
     2941 aaatgttcaa ggtaaagtct ctattaatga gtggaacgag cgtcgcagac ctgctaccct
     3001 caatgaactg gaaatcaaat tgtatcgtca atctcttgtt tattacgcag atcaagagta
     3061 cgagaaagcc accgatttat tgaaatttct aattgctcga acagagtata ctcgctttga
     3121 atatattgaa cgtttagcaa acatctatca tattatgaat gaacctgtca aagaatatca
     3181 gttattggac accgtcttgt cagtagcaga actgattgcc cttcctgctg gattagaaaa
     3241 gaaactagta cgccgccttt tacgagtaaa gcaacaacta tccgatcaag aaaaataact
     3301 tgcgtttttt taggaaagtg ctatgattat tttgttgaat aaatgggatg tagccaaact
     3361 ggtaaggcac ttgactctga atcaagcaat tgtaggttcg aaccctgcca tcccaatttg
     3421 atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa tctttttgca gggggattat
     3481 tgtattattt ttactacttg tatttagttt acataattaa attatgaata ataaaatcgg
     3541 gcgtattctt aatacaaaaa ctgatttacc caataattgc taatgctttc tccggaactt
     3601 tttctctagg aacttcctca tacgtttcta tgtgtgctcc tttattgcta attttcaaat
     3661 aatgattttc ttttaacaca gaaagaccat taaattcaac cgttcgttct ttcccattgg
     3721 catcagcagc tttctgcaca taccttgctg taccatattc atttacttcc tctggttttt
     3781 gtgtctttac atagacctct cctttttcaa ctaatggatt tagctgatca agtataccag
     3841 ggatttctcc ataacttcct tcagtataat atttcagtcc aaatagcaaa ataccactaa
     3901 tcgccgccag tccaatcaaa tatttaatca ctttcatctt tctacctcct tgttatatat
     3961 tcagtatatg ggtcagttaa agaaattcca tccgtcccaa gttggagatt tttctcctac
     4021 tttagaagcg agaatcgagt gtttttttag taaaaatgat cgttatttga tattattgac
     4081 gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa aatcatgttg atagcaaagg
     4141 atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt caaggaaatg attaagaacg
     4201 gcaatttcga tggcgcaaaa cgatttatcg aagaacataa agaacaatta ggaccttata
     4261 aagagaaagc ccaaaatctt ttaaaagatg taaatatcga cagtgtcaaa aacaaattta
     4321 aaaatctatt caaataagta aaactttcac ttttgtaaaa tcaaaggagg acaatgctgc
     4381 tgcatcgtcc tcctttggtt cttttctgaa tacttcatac taaaataaac gtgtcggatc
     4441 gtaatgataa gtcacattgc cgttcagtga ggcttttccc accacatatt tacgattctc
     4501 caacttcagc cacgcttctc gaaaagccat taattcttct ctttttacat caatcttttc
     4561 gatttttcca tcgatcaaat cttgaataag ttgttcatat ggattcattt ttttcctcca
     4621 tctgctgtca atcaataagt tgtaaaaata ggttcatatg cttttttggt atctagatct
     4681 tctctgactt tgactgtcgt ataaataatt ttttctactg ggatactata ataacttcct
     4741 tcaccattat tgaatcggtg aacgtctttc tttgctaaaa taccatttat tttattcgtc
     4801 agtacacgtt gttcgacact gttacttaca acatagtaaa tcacccgaat attattgatc
     4861 aaatgcaaat cgatttctaa cactacatga tccatgaaaa tcccactcct tctttcactt
     4921 tgattatagc aagtttctcc tctaatttca aaatcgatac agattttacc ggataaatcc
     4981 acaaaactgc ttgcgccatt tcgcacaaag tgttattttg tatcatggac tttatttatc
     5041 ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt atattccttt ttttattatg
     5101 ataagcattc ttttctatta cataaaatgg acaaaaaaga agttttcagt tttactggcc
     5161 tctttacctg ctgtatattt tacttatcaa attttttctt ttaggcattg ggaaacaacg
     5221 tctgtattaa taacacacat catcgaattg acgctttcag taatttttct tattatttgg
     5281 atctattttt tatataagaa tcagaattga atccctcgca tgtttaaaaa gaaaacgatt
     5341 aaagatgcag caaatttctg ccgatcttta atcgtttttt ctattctgtt ataccaaaaa
     5401 aatgtttgaa tcggtgaagg atcgttcctt gttttaattc tataaattct tgtgcgatcg
     5461 ggtacttttc ttccaaattt tctttgttct tttcgatcag gcttttcgct tcttcatatt
     5521 ttttatctgc acatagtgct tttacttcct gtaatacttt tcctgcttcc atttgcctca
     5581 ccctcttgcc attttattgc tttaagtttt atttaagtta tacgcattat tctagtttca
     5641 tcccaaataa ccttttttca tttttactcc tcgaactaat cagttttcta aaggctgatt
     5701 ggttcgtctt ttttattttt cctccatttc attctgctct tctgttaatt tatctaatgc
     5761 tttgattgat gctgcggctc ttgcataatc atagctgtat aattccataa atttatcagg
     5821 cagaacaaac cacgctggat gataaccgtt agctgtgaca gggaatcctt tatcttgttg
     5881 gttatctcca tcccatctca gagcaacacc tttttttcct ttccacttta ctaaagcgat
     5941 cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt gctaaaattt ctattttttc
     6001 ttttggcgca cggacttctt gaggacgaac cattttctat tcctccctta tctcattact
     6061 tcacgaccaa cacactgcaa ggggcatgtg atacgacata agaagcggtc gatccaacta
     6121 atgcttgttg tatagcacct tttcctgtcg ctcccataac gatcaaatca atattttctt
     6181 gtccagggat gacattggca atcaaatttc ttggatctcc agtttcaact attgcgtgga
     6241 tctcagcaat gccgtactct ttggcatcat tgattttttt cagcatggct acttctgttc
     6301 tttgtttttc atctgcatag atttttgaaa aggcataggc actatgggtc agctcaactt
     6361 tgttgatgat tgacacgata aataagtggg cttcattgtc ctttgctaag tcaagggctt
     6421 cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc taccatgatg ttctggtatc
     6481 ttttactcat tggaaccgcc tcctttttct tttattgtaa catctcaagc aaaaatacaa
     6541 aaaatatacg caatatgtaa ataaaatgat gaacaaaagc aatttctttc aagttattta
     6601 tacatttctt ttctcatcag aacacctgtt ctaatcccat tatttctgca ttatgtgaaa
     6661 ttcctcatca tttttcacat cgagcgaaca tcattctgtt acaaaaaacg tggatttgtc
     6721 ataaattgtc atgcttcttt tacctatgaa atattttcac gtgttaaagt attcctcgtt
     6781 gaacacaaca aataaaactt tttaacgaat aaaggagatt tttcatgaca tcacttaaaa
     6841 ctttactttt tggaacaact ttggctgccg gcgctgcatt cttcatggga acgactgctc
     6901 atgcggacga agcttatacc gttcaatcag gtgatactct atcaacgatt tctcaaaaat
     6961 acgttggtga taactccttg atcaatgcca ttgcagaatc aaattc
//
LOCUS       DACEPD010000111         6968 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.117, whole genome shotgun sequence.
ACCESSION   DACEPD010000111 DACEPD010000000
VERSION     DACEPD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.117"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1023
                     /locus_tag="IW797_002455"
     CDS             <1..1023
                     /locus_tag="IW797_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1225207.1"
                     /translation="ELKPKHERQNFLVGDDRLDHAVAFLWKDPQTGETVGASYQGTIV
                     DFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKFFESSIDLLSYAALNREKLQDAW
                     LVSMDGLKHHVISHYVEESISELRRKQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDP
                     FTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVS
                     AHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKVCERADGTYNFDRFYSRKANIKD
                     VNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1034..3043
                     /gene="topB"
                     /locus_tag="IW797_002456"
     CDS             1034..3043
                     /gene="topB"
                     /locus_tag="IW797_002456"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1225208.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            3389..3985
                     /locus_tag="IW797_002457"
     CDS             3389..3985
                     /locus_tag="IW797_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1225209.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            3998..4897
                     /locus_tag="IW797_002458"
     CDS             3998..4897
                     /locus_tag="IW797_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1225210.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            4900..5031
                     /gene="ssb"
                     /locus_tag="IW797_002459"
     CDS             4900..5031
                     /gene="ssb"
                     /locus_tag="IW797_002459"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1225211.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            5053..5385
                     /gene="ssb"
                     /locus_tag="IW797_002460"
     CDS             5053..5385
                     /gene="ssb"
                     /locus_tag="IW797_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1225212.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            5430..5663
                     /locus_tag="IW797_002461"
     CDS             5430..5663
                     /locus_tag="IW797_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225213.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(5822..5944)
                     /locus_tag="IW797_002462"
     CDS             complement(5822..5944)
                     /locus_tag="IW797_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ1225214.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            6134..6358
                     /locus_tag="IW797_002463"
     CDS             6134..6358
                     /locus_tag="IW797_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225215.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            6801..>6968
                     /locus_tag="IW797_002464"
     CDS             6801..>6968
                     /locus_tag="IW797_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ1225216.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYG"
BASE COUNT         2485 a         1236 c         1471 g         1776 t
ORIGIN      
        1 gaactaaagc cgaaacatga acggcaaaat tttttagttg gagacgaccg tttagatcat
       61 gcggttgctt ttttatggaa agatccacag acaggagaga cagtaggggc aagttatcaa
      121 ggaacgatcg tcgattttaa tcgtttcggt aaacggggaa cgtataaaca cattgacaag
      181 aatcctacgc caaatcacgg atttaattta aaaataggcg atccgaagca tttaaaattc
      241 tttgaaagta gtattgatct gttaagttac gctgcattga atcgggagaa actacaagat
      301 gcctggttag tttcgatgga cgggttaaaa catcatgtaa tcagtcatta cgttgaagag
      361 tcgatttctg aattaagacg aaaacaaaca tttcctcaat cgattgaaat atgtgtggac
      421 aatgatcgtg caggacatat tttttatgaa aaagaacaga tgaagggaat cgttgatcct
      481 tttaccaata agaaaatccg ttgtgagcga ggaattccaa atgattggca agtacctaaa
      541 gaatacaaag ctacttatga agcagtcgca aaagaaatga gtgttgaacc tgaagcaatt
      601 atggccatac acaaaactga aactaattta cagctgacaa atcaattggt gtctgctcat
      661 gatgttcaat ctacgtttgg aaagatgttg gcgaagggcg aaccagtaga aacaattgat
      721 ttgaaagaag catgtaccac ggtcgcgaag gaactgaaag tatgtgaacg cgcagatgga
      781 acatacaact ttgatcgttt ttatagtaga aaagccaaca taaaagacgt taatgcagga
      841 atccttcttt cctataaagc agaacaatac tacaaaggct ataaaaagca tgaacatgaa
      901 tttgtacctg aagtgaaaaa ggattggaat gatcaattga agcatgagat ccagcaacaa
      961 gaaatcagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca agaaagggag
     1021 tgaagtcgtc actatgaaaa ccgtcatttt agcagaaaaa ccgtcacaag cgaaggctta
     1081 tgccgatagc ttttctaaag ctacccgaaa agatgggtat tttgagatcc aagatcgatt
     1141 gtttacagga gaaactgtga tcacgtatgg ttttggtcat ttagtcgagt tagattcgcc
     1201 cgatatgtac gatgaaaaat ggaaacaatg ggcattagaa catttgccta ttttccccaa
     1261 tcaataccac tatcatgttc ctaaagataa gaaaaagcag tttagtgtcg tcaaacgaca
     1321 gctgcagtca gctgatacga ttgtgatcgc gactgatagc gatcgtgaag gggagctgat
     1381 tgcctggtca atcattcaac aagcaggcgc caatcaagga aaaaccttta aaagactttg
     1441 gatcaactcc ttagaaaaag aagccattta tcagggattc caacagttac gagacgcaga
     1501 agagacctat ccaaaatttg aagaagtaca agcacgtcag atcgccgatt ggttaattgg
     1561 gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac caggaagttt
     1621 ttctttaggt agagtccaaa ccccaacact ctatatgatt tatcaattgc aggaaaagat
     1681 tagaaacttc caaaaagaac catattttga aggaaaagcc caagtaatcg ctcaaaatgg
     1741 cgcatttgat gcgaagcttg atccaaatga aacccaagcg acacaagaag catttgaagc
     1801 ctatttaaaa gaaaaaggtg ttcaattagg aaaacagcca ggtacgatcc ttcaagtcga
     1861 aacagagaaa aaaagtgcgg ctagcccacg tcttttttct ttatcgagcc ttcagtctaa
     1921 aatgaatcaa ctcatgaaag ctagtgccaa agatactttg gaagccatgc aagggttgta
     1981 tgaaggaaaa tatctaagct atccgcgaac ggatacccct tatattacgg aaggggaata
     2041 tgcgtatctg ttggatcact tagacgaata caagcatttt ttaaaagccg aagcgattcc
     2101 gactcctatt catacaccga atagtcggta tgtgaataat aagaaagtcc aggaacatta
     2161 cgcgatcatt cctacaaaaa cggttatgac agcagcggct tttgaacaac taagtccact
     2221 gcagcaggct atttatgaac aagtgttaaa aacaacggtg gccatgtttg cggaaaagta
     2281 tacctatgaa gaaacaacga tcttgacgca agttcagcag cttcagttaa aggcaatcga
     2341 aaaggtgccg ctagatctag gttggaagaa gttatttgga aaagagagcg acggaaaaga
     2401 gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga cggtggatct
     2461 acaagttctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta cgttgatcac
     2521 tgcaatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca tattgaaaga
     2581 agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct tgaaacaaaa
     2641 ggaatatatc aaggtagaga aaaataagct cgtggtgacg aataaaggga ttttgctttg
     2701 ccaagccgtg gaaaaagagc cgctgcttac gagcgccgaa atgaccgcca aatgggaaag
     2761 ctacttgtta aagatcggtg aacgaaaagg aacacaaacc acttttctag ccaatatcca
     2821 aaaatttgtc tctcacttat tggaagtcgt accagggcaa atccaatcca ctgattttgg
     2881 atcgacgtta caagaagtga aagcagccag tgaaaagcaa gaagccgcgc gtcatttagg
     2941 aatttgtcca aaatgccagg aacaagaagt cttactctat caaaaggtgg cagcttgtac
     3001 gtctgaagcc tgtgatttta aaaggtcaga agggttcatt tgaagcaacg attgttttaa
     3061 aagaagactt cactacaggc tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa
     3121 gaacacgccg cactgcaaaa taatggaggg ttaatcttat gcgtaatacc acacacaaac
     3181 aacaagaaat cgaaaatcaa aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca
     3241 gcaaacgaag actgcagcaa ttgaagaacc agcagaggat tcaaaaagaa atcgaattag
     3301 atcgataaaa tcttcctaac tattgtatac gtataaactt atgttatact gtttttataa
     3361 gtttatacgt atacaaagga gggagaaaat gacaaaagca atggtataca agctgctaga
     3421 agaatttaat ggaactttgg ctttgaagga tgccaaaaaa gcgggcattt ctcctgtaac
     3481 gatcaaacgt atggtagatc gaggggagct ggatcgagag tatccaggtt ttttcacctt
     3541 acctggtcaa tttcctgatg aattgtttat ggcgcaaaaa aaatatgaac gcggcattat
     3601 ttcacatatt accgcgttag atttgtatga tttaacagat atgatcccta gacagatcga
     3661 tctgactgta ccttacggtt atcacgtttc tgaaaaagga ttgaaggaat tcgcagttga
     3721 gctacactat acaaaagcgg aatggtatga attaggtaag attgaaataa aaagtcgcta
     3781 tgggaatcct attatagctt atgatccaga aaaaaccttg tgtgacattt ggaatccctg
     3841 gtataacgtt gaggatgaaa tcaaagtaaa agcaatcaaa aactacatgg agtcggacag
     3901 aaaaaatctt agaaaattga atgagtatcg acgaattttg cctacagata aaacgatgcg
     3961 ctcttatatt atggctttaa attaaaggag aatacacatg acacctacgc aattaaaagc
     4021 aaaagtgaag aatatttcta gagaaaaaga agttgatccg cagcttgttt taagacattt
     4081 tatgatggaa aaattcttgg aaaaaatctc agagtctcct tatcgtgaaa attttgtgtt
     4141 aaaaggcggc ttcttaattg gctcaaagta tggcattgaa aatcgaacaa ctaaagacat
     4201 tgatacgact ttacgggaaa tgaaagtaac caaagaaaca ttgacaacgg ttctaaacga
     4261 tattttttca acgccgacga aagaaggcat tcaatttgag attcaaggaa tgaaagaaac
     4321 gagggaagct gattattatc cagggtttag cttacgtgta ctggcacatt tggaaaacat
     4381 gcggccagat tttaaggtag atgttactac aggtgactcg atctatcctg cgacgatcac
     4441 tcacagtcat aaattgatgt ttgaggatcg aacgattcct ttggaatcct atcctacaga
     4501 acaaattatt gctgaaaaat taagtgcaac ctttgatttt ttaacagata attcgagagg
     4561 aaaagatttt tatgatctct atactattcc taaaatggag aagatcgatg agaagacctt
     4621 gaaagatagt gtgagaaata catttacacg tcgaggaaaa actgatccgt taaagatata
     4681 ctatgaaaag gatatggatg tactgaaaaa tagtcacgag ctaaaagata cctggaagaa
     4741 atatcaaaaa agcaatcctt atgctgcgtc gatcacgtac gatgagacaa tggattcgat
     4801 tagtaatttg atggaaaaag tgattcacgt cgaagaaaaa gagcgaaaac tagcttatca
     4861 aagacaccaa cagatgaata gagaaatgga gagataaaga tgattaataa tgtgacatta
     4921 gtaggacgct taaccaaaga tcctgattta cgttataccg caagtggtac tgcagtagcg
     4981 acttttaccc tagccgtcaa ccgaaatttc acgaatcaaa acggcaattg agaagccgat
     5041 tttattaact gcgtgatttg gcgcaaacca gcagaaacaa tggcgacatt agctaaaaaa
     5101 ggtagcttga ttggtgtagt tgggcggatt caaacgcgga cttacgacaa ccagcaagga
     5161 caacgtgtat atgtgactga agttgtagca gataactttc aactattaga aagtaaagca
     5221 gcgacagaga gccgtgcgca cgctgatcaa tcgtccacca gtccaagcac cgccaccttt
     5281 gaacaacgga atacagcgac gcctaacaac aatggattga acgcttcaca gaatccattt
     5341 ggtggacaat caatcgatat tagcgatgat gatctaccgt tttagtttga gaaagaagga
     5401 gtaagcatgg caaaaaaatt agtctgacga tggatctatt gatacccgta attgcattta
     5461 tagacgggat ttctttgggc gtcactgaaa atcctttggt aaatagaatt gcgaaaggat
     5521 cactatttct tgtattcgtg tatcttgtct gttcttatct tttaaaaaga gcaggaaagg
     5581 ataattcctt ttggcgattg actaaagtca cagtagcagc gacagctgtt atacttacgg
     5641 ctattctacc gctttatatg tagagttaac gggaaatcgt tgaacttctt gcgaaatatt
     5701 gctataatga acatgaaaaa gaggagttgc gctaacaacc cctcattaac agcaccgatt
     5761 aaagaacggt ggcagaatat tagttatttg gaaataaccg ctgacctgct aaagtagacg
     5821 gttatttctt tttgtcattt ttaagcatat ttacaatcaa actaatcaaa gcaatggtaa
     5881 acataccaaa accaagaatg gtttgaattg tctcatatgc ggacaaaagg cttactcctt
     5941 tcatagattt tagcacttac attcataagc accaccacct ttccagaagt cgccaccgtc
     6001 ttcaaacttt actgtcttga ctattatagc atatctaaaa tcgaataaaa attctcaatt
     6061 ttagctgttt ttgctataat tttagcgagg tgatcgtgat ggaacaatat attggtttac
     6121 tcgataaatt agggtgatca agacacagcc attgcttgtg cgatttacat tacgcacgag
     6181 tcatgaacca atcaattgtg tagtggccaa tatagaaata gcggataaat tgctgatttt
     6241 ggaagaaggc aagtatacta tttcagtgac aggtcacttc aataaacgaa agcaactagt
     6301 cattgaatca atgcaaatta gaaaccctga tcattttacg cgatcgatga gcatataaaa
     6361 gaagagctgc agtcctaaat gaggattgta gctctttttg ttttacatag aaacagtaaa
     6421 aatgaaaagg cctgtttagg aaggcacaag cgccttctca attgaaagac ctttctctca
     6481 ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac gctacgcgtt cactaagata
     6541 aagggtcttg catagcaaga caaaatagaa aaaatttcct ctcaccaaaa ggttcgctcc
     6601 tgttcgttat ctctttattg atttgtcaga ccggcaacaa gttgcctttt atcaccaaaa
     6661 agcgacaagg acaagggtaa atggcacgct gcgcgccctt gtcttgtcgc ttttcgttga
     6721 ttcattctgg ccaacaaatc aataaagaaa gaactaacga aaggaagtaa aacctttcct
     6781 aagaggaggc agaatcaatc atgcgaaaga aatttgatta ttggggtgtt cccttcagcg
     6841 aattattccc aaactatcat gcaccacata ctgtcgaatg cgattgtgga gaaagagcga
     6901 aatgtatcaa aagctattgt ttgtatcaat gtccaacatg cgggaaaaaa tataccttgt
     6961 cttatggc
//
LOCUS       DACEPD010000112         6895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.118, whole genome shotgun sequence.
ACCESSION   DACEPD010000112 DACEPD010000000
VERSION     DACEPD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.118"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(3..455)
                     /locus_tag="IW797_002465"
     CDS             complement(3..455)
                     /locus_tag="IW797_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ1225217.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
     gene            605..1555
                     /locus_tag="IW797_002466"
     CDS             605..1555
                     /locus_tag="IW797_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1225218.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            1548..2507
                     /locus_tag="IW797_002467"
     CDS             1548..2507
                     /locus_tag="IW797_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ1225219.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            2504..3259
                     /locus_tag="IW797_002468"
     CDS             2504..3259
                     /locus_tag="IW797_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1225220.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            3282..4235
                     /locus_tag="IW797_002469"
     CDS             3282..4235
                     /locus_tag="IW797_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1225221.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(4758..5054)
                     /locus_tag="IW797_002470"
     CDS             complement(4758..5054)
                     /locus_tag="IW797_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ1225222.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            5410..5766
                     /locus_tag="IW797_002471"
     CDS             5410..5766
                     /locus_tag="IW797_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1225223.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            5776..6675
                     /locus_tag="IW797_002472"
     CDS             5776..6675
                     /locus_tag="IW797_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ1225224.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         2097 a         1175 c         1401 g         2222 t
ORIGIN      
        1 ttttaaaata aacgatccca gaagcttttt ttcttttttg gtttttcttt ttcaactggc
       61 gaagagtttt cttctgggta ttttttttcg atatcaatca caggttcatt tactgcttct
      121 tttgctgtta agagcaatcc gaattcctca ggatttgcag tgacgtaatc gttgactaca
      181 gtaaatcggc tattgttttt cgttgctagc tggatatatt ggttttggac agaaaggggt
      241 agagccccat tcaagagtat ggaagcatct ggatgttttt tgagttcttt taaaaagttt
      301 tctttgtttt ttggttggat catttcagca acagtcatac taagatagca gcgttccctg
      361 aatgtcccta aatatttatg ctgttcttca gggttcacta aaggtgtacc ataaagtccc
      421 ttatccaaat ggctttttac atcatcttgt gccataaaaa ataccctcct tcttctcttt
      481 gtctttctta cgattatagc atattcccaa taatacctaa aacatatgaa aaagcggttg
      541 cttttgttca acgattcact taaaataaga atgaaaatca ttctcaatta gaaaggtgga
      601 ttggatgcgc tacggacgac tagtagcatt attagttatt ttaagtagta tttcattgtt
      661 catcggagta tcgaatgtac aattgtcaga actgctccag ttagatgggc aaagcttaca
      721 aatcttgcta gtgactcgtt tgccacgaac gatttgtttg atccttgtgg gagcgaccag
      781 tagtatttgt gggttgatca tgcaacattt gacccaaaat aaatttgttt caccaacgac
      841 tgcgggcacg atggatagtg cgcgtttagg tattttagtg gctatgattt tcctacctgg
      901 tgcatccttg attgtccgat ccttgacagc attttgcttt gcttttgcag gaacattgct
      961 ttttttatcc cttacgcgat tgttccccca gaaaaatcaa ttgatgcttc cgttgattgg
     1021 agtcatgtta gggaatatca ttggttcagt tgctactttt tttgcttatc aattccagtt
     1081 ggtacaaaat atgacttcat ggcttcaagg aaattttgcg actgtgatac gaggaaatta
     1141 tgagttattg tatgctgttg ttccattgtt gctcttggtt tggctatttg cttaccgatt
     1201 tacgatcatt ggtctgggcg aatcgtttgc agtaaatttg ggaatagatt accagaagat
     1261 gcagttttta ggcattggct tagtatcatt ggctagtgca gtcatgttga tcatggtagg
     1321 aagcattcct tttatcggag tgatcattcc gaatcttgtc tctagaatat atggagatca
     1381 agtgcacaaa acaattggcg taactgcttt agcaggtagt ttgttcttag tattttgcga
     1441 tcttgtcgct cgcgtgttga tttttcctta tgaaatacca gtcagtgtga tcgttggaat
     1501 catcggtggg gctatcttcc tttatcttct gataaggggg cgacgatatg cttaaaaaaa
     1561 agccatctat ttttattggt atgcttgttc ttgttttatt tgttacagga atgtacttga
     1621 cgatccatac atatggaaac tggcattttg catgggcatt gaggagtaag aaaatttttg
     1681 catttatcct tgtctcgcta gctgtcagca gtgccacaat cagctttcag acaattacgc
     1741 ataaccagtt tttgactcct tcagtattgg gacttgagtc gttatatgtc cttgtccaaa
     1801 cggtcctttt cttctttatc ggaggagtga cgatgcttca gcagacaggt acagtttttt
     1861 tcttattgaa cgttgggctg atggtcgggc tgagtctatt gttatttttc tttcttttga
     1921 aaaaaatgag agcgaatctt tttttattac tgatgtcagg gatgatttta ggtacgcttt
     1981 ttggtagtgt cagtaccttt ttacaagtca tcatggatcc gaatgaatat gatttgcttc
     2041 aaggcagatt atttgcaagc tttggcaata tccagagtca gtatcttttg cctgcttctt
     2101 tgatcattat cggctgctgg tacctgttat ttcgatatcg acatgtgcta gacgttttcc
     2161 atctcggaaa tgaacaagca gtaaacctcg ggatagacac agatcgtttt gaatttttga
     2221 ttttatgttg tgtcagtctt ttgacaggga catcaacggc tcttgtgggt ccagttactt
     2281 ttttagggtt tattgtcgca aatatcagtt atcgtgtctt ttctacttat cgtcatggtc
     2341 agctatttat cggaagcttt ctggtagcta tgctgtttct gacaggagga cagctcgcag
     2401 tagagcagat tttcggctgg aatacaacag tgagtgtcgt cgttgaattt gtcggaggaa
     2461 tttattttgt cacaaaattg attcgcgaaa ggaagagtat cctatgattg atttacaaaa
     2521 ggtcaataaa ctgtaccatg gaaaatcagt ggttaaagat gtacagttaa cgattcctga
     2581 aaagaagttg actgctttta tcggtccaaa tggtgctgga aagtcaacgg tgttgtcgat
     2641 gatcagtcgt ttgataccaa aagatacagg tgaaatctat ttagaccata acgaagtaaa
     2701 ggcttggaaa tccaatgaat tagccaaaaa attatcgatc ctacgacaat cgaatgaaat
     2761 aaatgtccaa atcaccgtga aagaattagt agaatttgga cgatttcctt atacgaaagg
     2821 aaaattgacg acaaaagacg aggaaatcgt tcaggaatca ctggaacacc ttgggttaga
     2881 agaaatagcg gatcaatcga ttgacactct ttcaggcggt caactccaac gtgcgtatat
     2941 tgcaatggtg ctagcgcaag acacggatta tattttactg gatgaacctt tgaacaatct
     3001 agatatgaat tttgcggttc aaatcatgca gatcttaaaa aaattagtac aagaaatcgg
     3061 gaaaacaatt gttattgttc ttcatgatat caactttgct gcaagttatg cagacgaaat
     3121 tattgcaatg aaagatgggc agttattcac gcaaggaacg acccaagaaa tgataaaaaa
     3181 agatgtactg gacgaattgt acgaaatgga catacgaatt tgtgagattg aaggtcgcag
     3241 attctgtctt tatttctaaa actaacatta ggagagaaga gatgaaaaaa ttagtagtag
     3301 gtatcttgat tctagcatca tttggtttag cagcatgtgg aaatagtgac aacaccagtc
     3361 aggcagatac aaagaaaagc agtacgcaag ctgaaacggc attaacgata aaagacagta
     3421 atggcgatca aatcgaggtg ccaaagaatc ctgaaaaagt cgtcgtattc gacaatggtt
     3481 ctttagatac gatggatgct cttggtgttg gagataaggt agttggagca gctacaagca
     3541 gccttcctga gtatctttca tcctataaaa aagtggaatc agcaggtggg atcaaagaac
     3601 cagacttaga aaaaatcaat cagcttcagc cagacctgat catcatttct ggccggcaaa
     3661 gagattttca aaaagatcta agtcaaattg ctccgaccat tttcctttct ttagatgcga
     3721 aaaatccatg ggaatctttc cagcaaaatg tcactgcttt aggggagatc tttggtaagc
     3781 aagaagaagc taaaactcaa ttagaggaat tatctagtgc aatcgatcag acgaaaaaga
     3841 aagcagaagc tactgacaaa aaagcactag tcacacttgt gaatgaaggg caattatcag
     3901 cctacggatc tggttctaga ttcggtttca tccatgattt atttggcttt gaacaagcag
     3961 atgatcaaat cgaagcatcc actcacggtc aaagtgtttc ttatgaatat gtgctggaaa
     4021 aaaatccgga tatcttattt gtagtagaca gaacaaaagc tattggtgga gacgacagca
     4081 aagatgatat ttctgccaat gaactggtag cacaaacgaa tgctggtaaa aatcagcaga
     4141 tcatctccct tgaaccagat gtctggtatt taagcggggg cggtttagaa tcaatgaaac
     4201 tgatgatcga agatgtgaat caagcattta aatagcataa ataacagagg attaaactct
     4261 catactatgt aacaataatg tttcttacat tcttaaatag caagagccta tcctcaaaga
     4321 agttatcaaa aaccatccgt aaaatcgtat tgatgatttt acggatggtt tttatttatt
     4381 tgggaaatct tttttcaacg tgaattcatt ctctacatca tacgggttga ttctagaatg
     4441 gggagtggca aaaaatagtg tgatccattt tttcagcatc aagatcgcct cctttgattt
     4501 gaatcaaagg tacaaagcga aacttagata aaacttaaat ttagatattt ttattttgta
     4561 ccgatctctt ctttaaacga tgttccatct gctgcttctc ggcaataagt ccactagact
     4621 agaaaatatg agaagctatt ttctgtccag cgttcttatt acttaaagca aatcgtagtt
     4681 gtcacaatct cttctaaacg atgttccatc agctgacctt cggtattagc tcaaagcact
     4741 gatttcacaa cctctattta ggcatgtgtc tcagcaagcg gttgatagtt gatgatctgc
     4801 atttttcgat aaaaatcggc gggaagctta ctccaccgtt ttaataattg atagaaataa
     4861 ctctcttctg ctttgatttt atactcgctt aatatcaatc caaaactatt cgtttttttt
     4921 gaagctaatt ttttataaat atccaatgct ttggttttat cttcattgac aaagagtgtt
     4981 tttacttcaa tataagaacc ctcgatattt tgtgtttctt cgataaaata gatgtttttt
     5041 atatattcct tcatagtgat tcctccaaag aagtcttttt atttgataag tttactctac
     5101 cgaagagttg ttaataatcc gtgaacaaaa agtaattctt tttccaatag ttgttaaagg
     5161 ttttttattt tttatagtat tttttgttat ttaagaaagg aataaggtta aacaaataac
     5221 aggttgcttt ttgggtcttt ttccgttcaa gaaactgaac gaagaaaagg gaaagttgtc
     5281 tcttttgtga taagagaaat cataataaac gaatgactta ctttccaata aattgagaac
     5341 agtcagaacc agacaaatta gttgcatctg atgaataaag tgattattct atgacatgag
     5401 gtgatacaga tgaaattaga agtgacaaaa gaagcacaag aagtattgaa gaataaatta
     5461 gaagcagacg atcaactttt attagatatc gaaaacggtg acggtccatt tgcagaaagc
     5521 caagtcagtt gccaattgga cacggcattt cgattgatca ttgtcaaaaa agatacacaa
     5581 acagatctaa gtctttattc tgtagtggct gatacaccag ttggaccaat caaaatcaaa
     5641 gacagtgcaa tactttacat ggatgatcca tccacacttg ctttagaacc aacttataat
     5701 agtttacaat taaaaggacc aagcggcttg agaaaaggca acttgcaagt tgttcgaaaa
     5761 gactagaggg ataaaatggg aaaattggcg atcatcagcg acctacatgt ggacatcaag
     5821 aaattcggcg aatcagagct gatgcagctt tggcaagttt tgcaaaagca caagatcact
     5881 cggcttcatc tagccggaga tacggctaat aaagtagacc gttgcctagc tgttgtcgaa
     5941 tttttcgtca ataaaggatt atctacgaca tttcatttcg gcaatcacga gttggcagat
     6001 gtgcccaatg aacagatgat ggaccatttt ccagacccac attttttaaa tgaacggtat
     6061 attgctttaa atgagcagac cgttctttta gggatgaatg gctggtacga ctatcagttt
     6121 tccgaattga aagattacga ggcaatcgta cgaatgaagc gtttgtattg gtatgatcgg
     6181 ttcatccagc gtgggaaaac agatctcgag gtcaatgcag aaatgcttgc agctgcaaaa
     6241 aatttgctag accagctgga agcaaaaaaa cttgaggtga ttttatccac tcatttcgtt
     6301 cctaaacggg catttatcat gtatcaatct gctccttatg agcgctggaa caaattaaat
     6361 gcatttttag gatcagaatc attcggagaa ttacttgacc gttattcaaa tatcaaacaa
     6421 gtagtttttg gccatacgca tcgtcgcttt gaagataaat ccatcggtca cacgatttac
     6481 agctgcagac cttttggtta ttattatgaa tggcagctga cccgagattt tgtcttgaaa
     6541 gaacagctgt tagatagcta tgaacccatg aaattacgtg tcctcttgcg tgcccatcat
     6601 ccggcctttc aagcctatca aacacaacat ttgcaagagg aatttgaaca ggcgatgaca
     6661 atcgttccgt attgagacag cattagtaat gtgtaaaaga aaataccttg taaaacataa
     6721 ggataagtaa gagcagtaga tccatttatc gtggatatgc tgcttttttt gtggaaaata
     6781 atcagcgaat gtttatttga tcggatgatt tgtaactcgc agagtactag atgcaagcgt
     6841 ttattctttt tttgaacgat aatcacaaaa aaaaatataa tatacatttt ttatt
//
LOCUS       DACEPD010000113         6889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.119, whole genome shotgun sequence.
ACCESSION   DACEPD010000113 DACEPD010000000
VERSION     DACEPD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.119"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(89..649)
                     /locus_tag="IW797_002473"
     CDS             complement(89..649)
                     /locus_tag="IW797_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ1225225.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(671..2779)
                     /gene="mutL"
                     /locus_tag="IW797_002474"
     CDS             complement(671..2779)
                     /gene="mutL"
                     /locus_tag="IW797_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ1225226.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            complement(2797..5442)
                     /gene="mutS"
                     /locus_tag="IW797_002475"
     CDS             complement(2797..5442)
                     /gene="mutS"
                     /locus_tag="IW797_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ1225227.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            complement(5426..5839)
                     /locus_tag="IW797_002476"
     CDS             complement(5426..5839)
                     /locus_tag="IW797_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225228.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            complement(5817..6614)
                     /locus_tag="IW797_002477"
     CDS             complement(5817..6614)
                     /locus_tag="IW797_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ1225229.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
BASE COUNT         1721 a         1557 c         1221 g         2390 t
ORIGIN      
        1 ttagtcagag tagataagta atcttttcaa taaaaaaaac gggctatcat ggattttcaa
       61 tcgaaaatca gatagtccgc ttttttgttt aaatgaatgt tttcagtgcc tgattgacag
      121 caccaatcgg aaatcctacg atactgtaat aatctccaga aatttctttg acgaatacac
      181 tcgcagcatt ttggatgccg tatgcccctg ccttatccgc ataatccccc gtctccaagt
      241 agcgtttgat ctcttcgtct gtcaaaggat aaaaagtgac atgtgcttct gctagtatcc
      301 gttcttctcg ttcctttgtt tttaaaacaa cagctgtata tacagtatgt gtttccccgc
      361 ttagtttttt cagcatgaat tcagcctcag ccagatcttc tggtttaccc atgatctctt
      421 gattgagaac aacagtcgtg tcactggcaa tcactaaatg gtcttctttt tcatcttgtt
      481 cccagacaat tcgtgctttt tcttcagcca ttctacgaac ataatcgacg ggctgttcgt
      541 ctggaaatgg cgtttcatcg atatcagcag gttcaatctc gaattctgaa acgatatgag
      601 ccaataactc ttttcttctt ggtgattggg atgctaatac aatcttcatg ttttccctct
      661 tctctttgac ttaatgcggg tcttggattc gtttgaacat cctttccata tccgaattgg
      721 taaagtgaat caatactgga cggccatggg gacaattgaa aggattttcg catcgtgcca
      781 gatccttcag cagcacacgt gcttgtgctt cattcaaata atggttggct ttaatcgatc
      841 gtttgcaact catcatgatt gctgttgctt cacggaattt ttttacactc acacttccag
      901 ttgtcagcag catatcgatc atctcccgaa taatcgattc ttcttctcca ttgggatacc
      961 aagtaggatg ggcccgtaca ataaagctgt tttgaccaaa ttcttctaaa taaatccctg
     1021 catcttctag caagtgtttt tgctctttta atttcaaagc atcactgttt ggatagtcta
     1081 acacaaatgg aacaagcaat tcttgcagat catcagatac ttccccaatt ttttcccgga
     1141 aatattcata cttaatgcgc tcttgggcag catgctgatc aatgatgtac aacccatcgc
     1201 tactttgggc aaaaagatac gttccgtgca tctgtccaaa atattccaac tgaggaaaac
     1261 gttgtactgg tttttccgtt tctaatttct tgatggcttg ttctaatgtt ttgtcgtttc
     1321 cttgagtgaa atcaaattca ggatggagtg tcaattcttc ctcgtaaatc ttttcatcct
     1381 cttctacgga tttatctgct tgttcttcat ttgttttctc catgttatcc acaacgggaa
     1441 attctttctt ttcagatttt tccactgttt tttctacaaa gttatcaaca ttttcaccaa
     1501 acttatccac cgttttcgct gttttttctg gataagtggt gttttcttca gaaaaaccag
     1561 aatagttttc aacattttcc acagacttat caacaattct gtcaacaaat gtgttctttg
     1621 gttcttctac gaaaaaggta cctgtctctc gatcaaaatt caaactactt ggctttttag
     1681 gtttttcttc ttcaaaagcg taggacaaat cgatttcgat ttgttctgtt ttaggctgat
     1741 caggtacttt tttcttaaac cgtagattat ccgctgcact tggaatcaga ttttcttgag
     1801 acaatgcttt ttgaatggcg ttactaatta gctctgttag ttctttttcc ttcgacagcc
     1861 ggacttcctg cttcgtcgga tgaacattta catctactaa aagcgggtcc attttgattt
     1921 ccaatacagc aatagggaaa cgaccgacca ttaattttga cccataacct gcaacgattg
     1981 ctttattcaa ggaaaagttc ttgataaaac gtccattgat gatcgtcgat aagtaattcc
     2041 tactggcacg agtcacctca ggcaatgaaa catagccaaa taactcaaaa tctagatctt
     2101 ttccttcgat tttcagcatt ttttttgcgg tagataatcc gtagattccc gcaattgttt
     2161 gtttcagatc gccatttcca gcagttgcca gcattttgtt tccatcatgt actaaacgaa
     2221 aagcaatcga tggatgactc aatgccagtc gattgacgat gtccccgatg ttcgctaatt
     2281 ctgtgtgcag tgttttcacg tatttcaagc gggctggcgt attgaaaaac agattgctga
     2341 cggtaatctt cgttccttga cgcagtggtg ctggtcgatg ctcaataact tctccgcctt
     2401 tcagcgacag ataggtgcct tgtttttctt cctgattcgc cgtttccaga gtcatttcag
     2461 aaacagaggc aatacttggc aaagcttccc ctcgaaagcc cagcgttcgt atgcgaaaaa
     2521 gatcgtcgcg attatggatc ttacttgtcg catgccgctt gaaggcattc tctacgtcgt
     2581 ctgccaaaat gccttctccg ttatcaatca cttggatcgt ccgcagtccc gcttcttcaa
     2641 ctaagatatc aatctgagta ctgccggcgt caattgcatt ttccactaat tctttcacaa
     2701 cggaagctgg tcgctccact acttctcccg cagcgatctg attagctaaa cgttcagata
     2761 attcttggat tttggccatc gatatccgcc ttcttcctac agttgttttt gtaattcata
     2821 taatgtgttt agtgcatcca ttggcgtcat ttctagtaaa tttatttgct tcagtgattc
     2881 aatgacttct gtttcatttt cggataattc actgaataaa gaaagctgtt ggttttcttc
     2941 ttttacacgg tcttgtatat ggtcttgatt tactttttct gcaggtggtt ttatttgctc
     3001 ctgtttggct gttttagttt tctcttctac ttcagctgga acagtatggt gtccgttatc
     3061 gccattttct agcgtatgca ggatatctgc tgctcgttct agtagattgg aaggcaatcc
     3121 tgcgatcttt gccacatgta tgccgtagct tttatccgca ggtccatcca tcattttatg
     3181 caagaagaca acttctccgt ctttttcaac ggctccaaca tggacatttt taagctgcgg
     3241 aagttctttc tcaagtaccg tcagttcatg ataatgagta gaaaagagtg tttttgcttg
     3301 tacatgacga tggatatatt cgatgattgc ttgggccaac gccattccgt cataggttgc
     3361 tgttcctcgc cctagttcgt caaacaaaat caaactgttt ggcgtagcat aacgcagggc
     3421 ttgattggct tccatcatct ctaccataaa agtactttgt ccagcaatta agtcgtcact
     3481 tgcaccaatt cgggtaaaaa tccgatcaaa aataggtaaa actgcttgtt ttgccggaac
     3541 gaaacagccc atttgcgcca tcagaacagt taatgccaac tgtcgcatat acgtactttt
     3601 accagacata ttcggtcctg taatcagcaa gatcatttcg tcttctgcca tttctacgct
     3661 gttagggata tattcctgat gtcctaatac tttttctact acaggatgtc gtccgtcttt
     3721 gatcagaagc tgatgcttgt cactgacaag ttctggccga acatactgat agcgttcgct
     3781 gatcgttgca aaactttgta gcacatctac tgcactgatt gcttttgcaa gtacttgtag
     3841 cggctggata gcctttttga cttcttctct cacagctaaa aacaactgat attctaaatc
     3901 aacagatttt tcttctgctt cgaggatttg tgtctccaat tcttttagtt ctggtgtgat
     3961 gaaacgctct gcatttgcta aagtttgttt tcgttcatac ttctccagtt ctgcatttcc
     4021 aagatttgct tttgtgattt ctatataata accgaaaacg cggttaaatc cgatttttaa
     4081 gtttttgatt cctgtttctt gacgctcttt tgcttcaagt tctgccaacc atttctttcc
     4141 gtttctcata gcagaacggt acatatcgag ctgctcattg aatccatctt tgatgatgtt
     4201 tccttccgtt atttgtaacg gcggatcatc ttggatagct tggtcgatca attggacgag
     4261 atggtccatc ggctgcattt ctgaaagcag actttgccat tgaccttgat cgattccttc
     4321 aatcaaccct ctgatcattg gcacttgttc taaagaattt ttcagctgga tcaagtctcg
     4381 cccattgaca ttaccgaaag ctactcgacc tgctaggcgt tccagatcat agactttcgt
     4441 taatgctgac tgcaaatcga tacgttcaaa atagctgtct aacaaagaag cgaccatttc
     4501 ttgtcttgct ttaatttgtt ttgcctggat aagcggacgg tctagccatt gttttagtaa
     4561 acggccaccc attgctgttt ttgtttcatc taacaaccat agaagagtgc cgtgcttttt
     4621 cccagtccga atagattggc ttaattctag gttgaatttt gaataataat ccattttcaa
     4681 aaaatgatct ggttggtatt ctactgcttt ttggatatga gctaaactcc gcttttgtgt
     4741 caccgataaa taagtcaata gtttgccggt tgcttctttt ttcagcggat cgactagttc
     4801 gcttgtcaaa aagctaaatt ccgcgttttc ttccatctca ttttgttgag aaaagactag
     4861 ccctaaacgc ccgcttagct gttcacgaag ggtatcagtg atctcactgc ctaatacgag
     4921 ttcttttgtc tgcaatgcag aggcttcatt taaaacagct tcttcatcat gcaaccgagc
     4981 cgtcttcaat tctcccgtac ttaaatcggc ataagcaaag ccaaattcat ttccttgact
     5041 aactacagct gttaaaaaat tgttgtcttt tgccgacaat cctttgctgt ccatgactgt
     5101 accaggagtg actagctgga tcacttcgcg cttgaccatc cctttagtcg tctttggatc
     5161 ttctacctgc tcgcaaatcg ctactttata gcctttttca atcaaggcat cgatatatcc
     5221 ttgtgctgca tggtgaggta caccgcacat aggaataggt tcttctgcat ttcgatttct
     5281 gctcgtcaac gttaattcca aaagctgtga cgctttgatc gcatcttcgt aaaatagttc
     5341 ataaaagtct cctaaacgat agaacaaaaa agcatcttgg tattgggcct tgatgcttag
     5401 atattgttcc atcataggag tatttttagt tttttgaggc atgtttttcc tcctctattt
     5461 gtccattgat ttcttcttgt atcatagttg ttaaatgatg aagcagatca tctgcttcca
     5521 acagctgttt tctataagct acaacaagcg gatgctgatc aaattcttgc atgaattgat
     5581 ccgcttgttt gattgcttct tgttctgctg ctggtttgtc atagtgggca aaatgaacag
     5641 catctttttg cgcttgtttg attttttctc tcaactcagc taaatactgg ttttgctgga
     5701 tcttttcttc aagttctttg tagcgaatga tcgtttcatt ctctcttaaa agttttgtca
     5761 gtttccttac ttctttttga atttccggct cctgctgcaa aaaactgcct ctctttctac
     5821 tccatgaacg gtcgatcatc gttgatctga atcagctgga ttttttttgc atgacctgtc
     5881 gtatcatcca attccaagat acagccgctt aaaatcgatc ggccttgttc cacaacttca
     5941 aaacgttttg gtaatgcggt cagaaacttt tcgattacag gttctctgcg catacccaaa
     6001 ataccatcat agggacctgt catcccaacg tctgtcagat acgctgttcc tcttggcaaa
     6061 attcgcgcat catttgtctg aacgtgtgta tgtgtaccga ccactgctga aactttccca
     6121 tctaaaaacc agcccattgc ttgtttttca cttgttgttt ctccatgaaa atctacaaaa
     6181 atgatcggcg tacgttttct ggcttcttct accagctcat tcattttacg aaaaggatca
     6241 tccaagtcca ccataaaaga acgagcctgc atgttgatca ctgccagttc gacttggttc
     6301 actttgacaa agaccatccc ttgtccaggc gtcccttccg gaaaatttgc cggacgaacc
     6361 atctttttag cttcatttac aaactcaaag attccgcggt tgtcccacgt atgattcccc
     6421 atcgtcacga catctacgcc gtcttgcaaa aattttttat agattttttc tgtgattcca
     6481 cgtccagaag cggcattctc accatttaca atcgttactt gcggacggta cttcttcttc
     6541 aatctaggaa gatattcagt aatcatctct cgtcccattg aacctactac atcaccaata
     6601 aacaataccc gcacactaaa ttcctcttct cttttaacat cactctatat tatagttgtt
     6661 ttcaataaga aagaaaaggt caaacaaaag gaactctttc ctggtatttc ttagttttga
     6721 aaaataaaat aaaaaaatgg gactatgaca aacatcttgt cacagtccca tttcccgaat
     6781 aaacgttgtt tcatctactt cttcagaaat aagccgtaat tttacaaaaa tttgagaagc
     6841 aattttcgta aatcccttct tatttcttga aactgaccac ttctgtcac
//
LOCUS       DACEPD010000114         6866 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.121, whole genome shotgun sequence.
ACCESSION   DACEPD010000114 DACEPD010000000
VERSION     DACEPD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.121"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..813
                     /locus_tag="IW797_002478"
     CDS             <1..813
                     /locus_tag="IW797_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAQ1225230.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="IW797_002479"
     CDS             807..1229
                     /locus_tag="IW797_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225231.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            1531..1911
                     /locus_tag="IW797_002480"
     CDS             1531..1911
                     /locus_tag="IW797_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ1225232.1"
                     /translation="MSEQNQNLASDHSKKYTYRSKPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            1893..2807
                     /locus_tag="IW797_002481"
     CDS             1893..2807
                     /locus_tag="IW797_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ1225233.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            3047..3736
                     /locus_tag="IW797_002482"
     CDS             3047..3736
                     /locus_tag="IW797_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225234.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKV
                     EEACSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(3856..3942)
                     /locus_tag="IW797_002483"
     CDS             complement(3856..3942)
                     /locus_tag="IW797_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1225235.1"
                     /translation="MFELFICPLLVGVCISVFSYWLNNRNKK"
     gene            4588..5520
                     /locus_tag="IW797_002484"
     CDS             4588..5520
                     /locus_tag="IW797_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1225236.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIH
                     GEVDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEAD
                     FKKQQQTEKEEAEKQSISNELKQDLLENLQNLFD"
     gene            5537..6007
                     /locus_tag="IW797_002485"
     CDS             5537..6007
                     /locus_tag="IW797_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ1225237.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTL
                     VVSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLD
                     QQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
     gene            6091..6408
                     /locus_tag="IW797_002486"
     CDS             6091..6408
                     /locus_tag="IW797_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1225238.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDT
                     ENLIQFAKLFDLSLDNILLGEEKIMVKNYGKKSYKNTNTIISFCNFFKFSFHLNSVRI
                     LLF"
     gene            6525..>6866
                     /locus_tag="IW797_002487"
     CDS             6525..>6866
                     /locus_tag="IW797_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAQ1225239.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2587 a         1152 c         1182 g         1945 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acatgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgaa
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc
     1441 ccttttggtg agctttactc attgtgcctt tttaggtaca atggtattgc gaacgatacg
     1501 tccgtaggac gtactgaaag ggtgggactt atgagcgaac aaaatcaaaa tttggctagc
     1561 gatcattcta aaaaatatac ctatcgatcc aagccaaaac aaatcagttt tcgagtgagc
     1621 gaatctgaat ttgcaaagct gaagcagtca gctgaagctt tgcaaatgag tgtgccggct
     1681 ttcgtgaaag ccaaggcaca aaacgcgcgt ctggtgacac cgaaagtggc gccagacatt
     1741 gcccaggcaa tcgcgcgcga tttagccaaa gctggaggga atatcaatca aattgccaag
     1801 tggtgcaata cacaccaaca tgacgttgct cctggagacg cccaacgttt atccgaaaac
     1861 ctaaaaatca tgcaaaagga gctacaaaag atatggcaac agttaaagta agtcgaacca
     1921 cttcctgtag ccgagcaatc aattacgcag aaccacgagc aaccattaaa acaggaataa
     1981 actgtgacgt cgattatgcc aaaagtgaaa tgaaacaaat tcgcatgctg tatggaaaag
     2041 acgaccgtgt acaggcacac ttattgattc aatcttttcg accaggagaa attaccgctg
     2101 aaaaagctaa tcaactaggg gcagagtatg caaaaaaaat agcccccgat caccaaacag
     2161 ctatttacac acatacagac aaagaccata ttcataatca cattgtcatt aactccgtta
     2221 atctggagac tggaaaaaaa tttcaagctc atggtcaagc atttctaaac aaatgttatg
     2281 acctaaatga cgagatttgt cttgcacata acttaccaat cactgagcga ggaaagaaac
     2341 cagaaaaacg aaccatgtct gaaataaaat taaaagaaaa aaatgaacct gtttggaaag
     2401 acgaaattcg gattgcgatc gatcaaacaa tgaaaaacaa aaaaaccaga acctatgacc
     2461 aattttgtaa ttccctcaaa acattgggga ttcattgctt caatcgcggg aaaaatttca
     2521 cttacgaatt gatccacaaa aagaaaaaag tacgatccaa cagactagga aaggactacg
     2581 aaaaggagac gattttacat gagctggaca gacgagaacg aacctataac caacagcgaa
     2641 ccaacgagct acgaagagct cgagctagca ttcgcgaaac tgcagaagaa gtacgaagag
     2701 aacaacgaac tcttacgcct gaacaaccgg aaattagacc ggctcgaaga aaccatgaac
     2761 aacacgttga aagaggaaca gaccaccaac aagaattatc gctctaaaac gttaaaagac
     2821 atagaaaacg aaataaagaa acaacgtgct tttctcgttc cttatacgca agaaaggata
     2881 caaactacga ttgatttaca agttaaaaaa ctccatcaag agctattaga ccagcgaaat
     2941 aaggaacaga aaaaggtgct tattcaacga tcattggatt ttcttgcccc cttatgttgc
     3001 ttagtcctga cactgttcgc attttggtta gtatggggag gctaaaatgg cattttattt
     3061 accctatcta ctcatttttg ttagtatttc aggaagtatt tggctcatct ataaaatttt
     3121 tcaaacaaga tattcattaa aaggctctaa aattcgattt aagcgatttt tctttcttgg
     3181 ctgtatattt tccttgataa tcgtttcaag cggcttattc ggcgttttag agggaaataa
     3241 gcgcgtttct agaagtattt tattaggaaa tgtcactcaa aagtatgaat cagcgcgtga
     3301 taaaaagaaa aaagagcaag cattagcaca aaaaatggaa gagtttacca cttgttatga
     3361 ggaaatgaac gatatttttg tgaatcaaga aaaacgtttg acggataaaa acatggaaaa
     3421 actcactcgt ctttatcaaa acttgccaga aaaacagcaa aaagaggttc aagacaacta
     3481 tgagcgaacc aaaaaagacg tgcaatatgt gaaagatacg aaagtggaag aagcttgtag
     3541 tgatttattt ggagatacaa ttcctttttc aactagcgaa caagaacgaa aagaacgaca
     3601 acaatcagtc acatatgaac gctatgaaat cttgcttcaa caagcaatta acatacaaaa
     3661 tctgactaaa aaagaaacag ctttaaatta tttaaaaagc gtcaaggaat ggttagatca
     3721 gcaacagcaa aattgaattt gattgaaatt catgttaaac taaacatgca aatgttcaaa
     3781 cattagatac acgaaagccc cagagaggtc ggattctctg gggctttttt taggttgatg
     3841 tcgcctttac tgattttact tcttgtttcg gttattcaac caataactga atacagagat
     3901 acaaactcct acaagaaggg ggcaaataaa cagttcaaac atcagataca cctccttcca
     3961 gtcgcaagac tgtcggatag gcgacaagtt attataccct acaaaattaa atctgtgtgg
     4021 caattttaag tatctcttat ttccgggttg ttagggcttg ccctgaccgc ctgtagggcg
     4081 ctttaaattc cagataaagt tttttatact aagactgtct taaaacggct tagacaagct
     4141 cttagagcct caaccctcct ttttgaacaa tggttttagc aaaggagctg gaggcgactg
     4201 cgcaggtata atgtgagagc gtagcgatca cacctttttg gcgaccgtag ggagcgaggt
     4261 tttgaattag cagtttagaa aagttcgcga acctgtttaa agaaaaaata attgtttttt
     4321 taggtgggaa attttgtctc cctcttttat aatctttttt taaacctctt tttaaacctc
     4381 tttaacgtcg gctgtgtcct tactccccca aggtatacaa aattttatag gcatgaaatt
     4441 atctgtttat aggcatgaaa ttatctgttt ataggcatga aattatctgt ttataggcat
     4501 gaaattatct gttttaggta ttgtttttag acataatatg tcctaaaatg atgtcaataa
     4561 aatcatttag actggagtaa caactaaatg gcaaatgaaa tcgtaaagta tgaaccagaa
     4621 ttaaatacca ttcctttaag aaaatttacc ccaatagaaa tgaacttgtt tttttctatt
     4681 gtctctagaa tgcgcgataa aggtaatcaa aaaatacgct tttatttcag ccaacttaaa
     4741 gatttaagtg cgtataagca gactgcagga gaagtcttta taaaagattt ggcaagaaca
     4801 tacgatcatt tgatggattt acgtttcgga agaaaaagca atggtggatt aacaatagaa
     4861 cgatttacaa tgtttactga ttttgaaatt catggagaag tcgataaccc ttatgtagat
     4921 ataaaagtcc acgaaaaagc tttgccttta cttaataatc tagaaagctg ggtacgtttc
     4981 tctttaacag aatttcgaga tttaaatagc agttatgcta aaacaatgtt tagattgcta
     5041 aaacaatacc gaactcaagg atgggctgaa ttttctaagg aagatttttt agagctatta
     5101 gatattccta aaagctatag acaaacaaat ataaatcagt ttgtactcaa accaataaaa
     5161 gaagaactaa ctccactatt taaaggatta actattcgta aaaaatatgg taaaggtcga
     5221 ggtaaacctg ttattggtta tcgttttact tggaaagctg aaatcaacca tgctgacgat
     5281 ttttcaaaag ggaagcaaga agatttacgt ataaaattat ttaatattga acacaatgga
     5341 gaattaacac aaaaagaaaa atggcgtgct aaagatcgaa tactaaatct tccgttaggt
     5401 actcatgaag ctgattttaa aaaacaacaa caaaccgaaa aagaagaagc ggaaaaacaa
     5461 tcaatctcaa atgagcttaa acaagattta ttagaaaact tacaaaactt attcgattga
     5521 agcataagga gtatttatga tagataaaac gatcaaagaa ctcgcggaag aatttcatgt
     5581 ttcaaaacaa gcaattagga aaaggctatc agctgatttt agagaaaact atgtgcaaac
     5641 tgttactagg aacggagtga aaaccttagt tgtcagctac aaaggatatt tgcttttaaa
     5701 acaacatttt ttaggtagta acaccactgg taaccaagta gaaaccgttg ctagtaacga
     5761 tgaaaactcg attattgttt ttttacatga acagctccaa gaaaaagata ctcaaataaa
     5821 acaaatgcaa aagttactcg accagcaaca acaactcacc ttgcaggcaa atcaacagat
     5881 cgaaaaactg caagaacagt tacaattagc ttatacggaa gaacctgaag aagaggaatt
     5941 aacttctgtc aataaagaaa acaacaatgc aataacaaca gaaaaaaagt ggtggaaatt
     6001 ttggtaacaa ttaccaactt tccataaaga agaaatcaat ttctatagac tctatctttc
     6061 taagaataaa aaatatgttt aggtgatcaa atgactataa gtaaagtaat caaacaaaaa
     6121 agaatagaaa aacaactaac tcaagaagat ttagctgaaa tgcttttagt atctaaaaaa
     6181 accatttcta attgggaaaa tggtcgaaca ataccagata cagaaaattt aattcagttt
     6241 gctaagttat ttgatttatc actagacaat attttgttag gagaagaaaa aattatggta
     6301 aaaaattatg gtaaaaaatc atacaaaaac actaatacta ttatttcatt ttgtaatttt
     6361 tttaagttta gttttcacct taattcagtc cggattcttt tattttagcc attcaattgc
     6421 cacttggtta ttaatatttt ttctaagttt actattaata attaatacat taataattaa
     6481 tactttaatt ataggtgtat accacttaaa tttaacacaa aggcgtgatt attttgaaac
     6541 ccaaaaagaa aatttatcta ggtctcctct tttccttgat gattggaatt tttagtatag
     6601 gaattacaaa tacctatgct tcagaaaatt ctaatttcaa tatcaactcc caaccaattg
     6661 aagaacaagc aattaatttt gcgaaaactg attttgaaca ctccataact tcaattcttt
     6721 taatggaaaa taacaccgaa actgatatta actatactct tggttcgcca tttaaaatgc
     6781 tcgatggaga aaatacggca aatagttcta ttttctttcc agtattttca gacgctgata
     6841 atacgattaa atatatttat accgtc
//
LOCUS       DACEPD010000115         6857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.122, whole genome shotgun sequence.
ACCESSION   DACEPD010000115 DACEPD010000000
VERSION     DACEPD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.122"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            127..579
                     /locus_tag="IW797_002488"
     CDS             127..579
                     /locus_tag="IW797_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225240.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="IW797_002489"
     CDS             593..1282
                     /locus_tag="IW797_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225241.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="IW797_002490"
     CDS             1407..3758
                     /locus_tag="IW797_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ1225242.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="IW797_002491"
     CDS             3838..4233
                     /locus_tag="IW797_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225243.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="IW797_002492"
     CDS             4558..5175
                     /locus_tag="IW797_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1225244.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="IW797_002493"
     CDS             5216..5515
                     /locus_tag="IW797_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225245.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="IW797_002494"
     CDS             5549..5716
                     /locus_tag="IW797_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1225246.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6324)
                     /locus_tag="IW797_002495"
     CDS             complement(5761..6324)
                     /locus_tag="IW797_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1225247.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(6352..>6857)
                     /locus_tag="IW797_002496"
     CDS             complement(6352..>6857)
                     /locus_tag="IW797_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1225248.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIK
                     VLMGITA"
BASE COUNT         2309 a          989 c         1356 g         2203 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttcggt
     6301 tctgttgcga agtttgaaaa tcaaacgcgc cctctctgaa ctccaaatat cttaggctgt
     6361 tattcccatt aataccttga tttcagtaga caccgaaaag ccgaagagcg ttccatttct
     6421 tcggttcttt ttatatattc ctcgaatggt ctccatgccc ttaatcgtgg aagaggctgt
     6481 acggagactt tgataaaatt tatttcgtcg tttaataggt cgatggtctt gttctattaa
     6541 attgttaaga tacttcacag ttcggtgctc tgtcttagta tataaaccca cactctgtaa
     6601 ctttctaaag gcggagccaa gagaaggtgc tttatcggtc acaattgctt tcggctcacc
     6661 aaactgttta tggagtcgtt ttaagaaagc ataggctgct tgcgtatccc gtttctttcg
     6721 taaccagata tctaaggtta agccgtccgc atcaattgca cgataaagat aatgccaacg
     6781 tcccttaatt ttgatatagg tttcgtccat tttccatgaa tagaaggatt gtctattttt
     6841 cttcttccaa agataat
//
LOCUS       DACEPD010000116         6689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.123, whole genome shotgun sequence.
ACCESSION   DACEPD010000116 DACEPD010000000
VERSION     DACEPD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.123"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1014
                     /locus_tag="IW797_002497"
     CDS             <1..1014
                     /locus_tag="IW797_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ1225249.1"
                     /translation="ELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQGTFI
                     DYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTW
                     LVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDP
                     FTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQLVS
                     AHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGDINA
                     QILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQ
                     ERE"
     gene            1025..3229
                     /gene="topB"
                     /locus_tag="IW797_002498"
     CDS             1025..3229
                     /gene="topB"
                     /locus_tag="IW797_002498"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ1225250.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            3470..4063
                     /locus_tag="IW797_002499"
     CDS             3470..4063
                     /locus_tag="IW797_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAQ1225251.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            4076..4975
                     /locus_tag="IW797_002500"
     CDS             4076..4975
                     /locus_tag="IW797_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ1225252.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            4978..5451
                     /gene="ssb"
                     /locus_tag="IW797_002501"
     CDS             4978..5451
                     /gene="ssb"
                     /locus_tag="IW797_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1225253.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            5817..6077
                     /locus_tag="IW797_002502"
     CDS             5817..6077
                     /locus_tag="IW797_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225254.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
     gene            6522..>6689
                     /locus_tag="IW797_002503"
     CDS             6522..>6689
                     /locus_tag="IW797_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ1225255.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYG"
BASE COUNT         2453 a         1167 c         1377 g         1692 t
ORIGIN      
        1 gaactaaagc caaaacatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
       61 gcggtcgcct ttttatggaa agatccacag acaaatgaga cagtaggggc gagttatcaa
      121 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
      181 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gttaaaattt
      241 tttgagagta gtattgattt acttagctat gctgcactaa atcgtgatca gttaaacgat
      301 acgtggttag tttcgatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
      361 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
      421 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agttgatcca
      481 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
      541 gaatacaaag tcatttatga agaagtcgca aaagaagaaa agatcacacc agaagctatt
      601 atggccatac acaagacaga aaacaatctg caattaacga accaattggt ttccgctcac
      661 aaagtaaatg cgtcttttgg tcagcagctt tctgttaacg attcaataga agctattaat
      721 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag cttgtgaacg cgtggatggt
      781 acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgctca gattcttttc
      841 tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga atttgtacct
      901 gaagtaaaaa aggattggaa tgatcaattg aagcatgaaa tctaccaaca agagatcaga
      961 aagcaaaaac gtgccatgtt gtttcaacaa ggtagacagc aagaaaggga gtgaaatcgt
     1021 catgatgaaa accgttattt tagcagaaaa accgtcacaa gcgaaggctt atgccgatag
     1081 cttttctaaa gctacccgaa aagatgggta ttttgagatc caagatcgat tgttttcagg
     1141 agaaactgtg atcacgtatg gttttggtca tttagtcgag ttagattcgc ccgatatgta
     1201 cgatgaaaat tggaaacaat ggtcattaga gcatttgcct attttcccta cacattatca
     1261 ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc aacttcaatc
     1321 ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga tagcttggac
     1381 gatcattcaa caagcaggcg ccgatcatgg aaagatattt aaaagacttt ggatcaactc
     1441 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagatgcag aagagaccta
     1501 cccaaaattt gaagaagcac aagcacgcca gatcgccgat tggttgatcg gcatgaacgg
     1561 tagcccgctg tatagtttac tcttgcagca aaaaggcata ccaggaagtt tttccttagg
     1621 cagagtccaa accccaacgc tttacatgat ttatcaatta caggaaaaga ttagaaactt
     1681 ccaaaaagaa ccatattttg aaggaaaagc ccaagtaatc gctcaaaatg gcgcatttga
     1741 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     1801 agaaaagggg gttcaattgg gaaaacagcc aggtacgatc caccaagttg aaaccgagaa
     1861 aaaaagtgcg gcaagtccac gtcttttttc tctatccagc cttcaatcta aaatgaatca
     1921 gctcatgaaa gctagtgcca aagatacttt ggaagccatg caagggttgt atgaagggaa
     1981 atatctaagc tacccacgaa cagatacccc ttatattaca gaaggggagt atgcgtatct
     2041 gttggatcac ttagacgaat acaaacactt tttaaaggcc gaagccattc ctactcctat
     2101 tcatacaccg aatagtcgat atgtgaataa taagaaagtc caggaacatt acgcgatcat
     2161 tcctacaaag acggtcatga cggcagcggc ttttgaacaa ttaagtccac tgcagcaggc
     2221 tatttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaagt atacctatga
     2281 agaaacaacg atcttgacgc aagttcagca gcttcagcta aagacagtcg gaaaagtacc
     2341 agtagatcta ggttggaaaa agctgtttgg caaagagagc gacagaaaag aaaaggaaga
     2401 agaaccgcta cttcctaaag tgacaaaagg agaagcggtg acggtagatc tacaagtcct
     2461 tgaaaaagaa acgaagccac cacaacctta taccgaaggt acgttgatca cagcgatgaa
     2521 aacagctgga aaaaccgtgg atagcgaaga agcacaatcc atattgaaag aagtagaagg
     2581 aatcggtaca gaagcaacac gagcaaatat tattgaaact ttgaaacaaa aggaatatat
     2641 caaggtagaa aaaaataagc ttgtggtgac aaataaaggg attttacttt gccaagccgt
     2701 ggaaaaagag ccgctgctaa caagtgccga aatgaccgcc aaatgggaaa gttacttatt
     2761 aaagattggt gagcagaaag gaacacaagc tactttctta gctaatatcc aaaaatttgt
     2821 ctctcacttg ttggaagtcg taccgggaca aatccagtca acagattttg gatcaacgtt
     2881 acaagaggtg aaagcagcca gtgaaaaaca ggaagcaacg cgtcatttag gtgtttgtcc
     2941 aaaatgtcgg gaacaagaag tcttgcttta tcaaaatgtg gcggcatgta cgtctgaagc
     3001 ctgtgatttt aaattatgga caacgattgc caaaaagaaa ctcacagcca cacaactgaa
     3061 agagatcatt caaaacggcc gaacaagcca atcagtcaga ggactaaaag gtcagaaagg
     3121 ttcgtttgaa gcaacgatcg ttttaaaaga agactttaca acaggctttg aattttctga
     3181 aaagaaaaaa acgaactata agaaaagaac acgccgcact acaaaataat gaaggggtta
     3241 tcctatgcga aatacaacac acaaacaaca agaaatcgaa aatcaaaaag caaaaattcg
     3301 tttagaacaa gagaaattac gtgcaaataa aagaagactg caacaattaa agaaccagca
     3361 gcgcattcaa aaagaaatcg aattagatcg atagacgtta atcttgttac aaataaacta
     3421 ctatgatata tttgatgtag tgtatttgta acgaagggag gggaagtaaa tgcaagataa
     3481 actgaaagaa ttgttggcta ttcgcgatgg aaatttatct atgaaggaag ccaggaaatt
     3541 tggaatcgca gcaaccactg tacaaagatt ggtgaatcaa ggaaaattga tcaaagttga
     3601 tcgtggttat tatgtagagg atggtcatgg aatagatgat ttattttggc ttcagcaacg
     3661 attttccagg ggaatcttct ctcatgaaac gactttagat atttaccagc tttctacgaa
     3721 tatgccgaaa ttcattcact taacttttcc ccatggctat aacgttgatc gttcaattac
     3781 aaaagagcag cagttacaat ttcatttcgt gaagaaagaa gtttatgaac taggaaaagt
     3841 agagggaact tcctttcaag gaaatcctat tactatgtat gataaagagc gtacactttg
     3901 tgatatttgg aatccgagat atgaatttga atatgaaatg aaattagaag cattaaaaga
     3961 gtacatggaa gacccattga gagatccaag taaattaaga gattacatga caaaactaca
     4021 tgtagaaaaa gaaatgaaat accacatgca aagcttatat tgatggagga aaaagatgac
     4081 accgacacaa tttgcagcaa aaacaagaaa tttagcaaag agtacaggat taaatgcaca
     4141 gttagtgcaa cggcatttta tgatggatcg tctaattgaa caaatttctg aatcaaagta
     4201 caaaaatgac ttcattttga aaggcggctt tttattagga tcaaaatatg gaatcgatag
     4261 aaggacaaca attgatatcg atacgacatt tagaaatagc aaattaactg aagaaaaatt
     4321 gaagagcatt tttaatgatt taactagaaa acccacaaaa gaaggcattg ttttctctat
     4381 tcaaggaatg aaggaaacaa gagaagccga tttttatcct ggatttcaaa ttaaattggt
     4441 ggctaattta gagaaagtac gtgtaccgtt taaaatggac gtgacaacag gagattctat
     4501 ttatcctgaa gtagatacac actatcacca attaatgttt gaagataaaa aagtggagat
     4561 tccagcttat ccaacagaac aaattattgc agaaaagcta agtgctacgt tcagctttgg
     4621 cacagataat tcgagagcaa aagattacta cgacttattt accattccga aactagaaaa
     4681 tatcgatacg aaagagttat acaaaagtgt tcgaaataca tttacaaaac gtggggatac
     4741 aaaaccgttg tccttttatt atgaaagaga aatggataag atcagaggaa atgactattt
     4801 ggaaaagcag tgggaaaaat atcgctcagt taacacgttt gcgaattcca tttcatttac
     4861 agatacagtc aacgaaattg atcacctaat gaaaagtgtg atcaaaattg aaaatcaaga
     4921 aagaaaacgg cagtttaaaa gatcacaaca aatgaatcaa gaaatggaga gataaagatg
     4981 attaataatg tgacattagt aggacgctta accaaagatc ccgatttacg ttatactgca
     5041 agtggcacag cagtagcgac ttttacccta gccgtcaacc gaaatttcgc gaatcaaaac
     5101 ggcaatcgag aagccgattt tattaactgc gtgatttggc gcaaaccagc agaaacaatg
     5161 gcgacattag ctaaaaaagg cggcttgatc ggtgttgtcg gacggattca aacacgaact
     5221 tatgacaacc agcaaggaca acgtgtgtat gtgactgaag ttgtagcaga taactttcaa
     5281 ctattagaaa gtaaagcagc gacagagagc cgtgcgcacg ctgatcaatc aaccaccagt
     5341 ccaagcccca ctacctttga acaacgggat acaaacaatg gattggacgc ttcacaaaat
     5401 ccatttggtg gacaatcaat cgatattagc gatgatgatc taccattcta agtagcattt
     5461 tctattggtt gcatttgcta cggtttatgt tataatttag tcaacaaaaa gaattttcaa
     5521 tctataacca aaaaccacta gctggcgggc tagtggtttt cttttacgtt tctattataa
     5581 gaattaacgg gtaggcttac tgtctttctt ggcaaattta caatcaagcc atttgcagat
     5641 atagtgcgcg gctacttctg ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc
     5701 tataacctcc tccccttgcc agattaggcc ggggtagcag acagcaagac tattatagca
     5761 taattgaaat attcaaatga ttggattttt gctataattt tagcgaggtg attgtgatga
     5821 acaaatatgt tggtttgctt gataaaatcc gagtaataaa aacgcaaccg ttattggttc
     5881 gctttacttt acaaacgatt catgaatcga ttaattgtgt agttgcagat attgaaatta
     5941 tcgataaatt gctaattatg gatgatggaa aatacaatat tgccgtcaca ggtcacttca
     6001 ataaacgaaa ccagctagtt attgctagca tgtatgttag aaatcccgat cagtttacgc
     6061 gattgatggg catataaaaa aagaagagtt gcaatcctaa tgtaggactg cagctctttt
     6121 tgttttacat agaaacaaga aaaatgaaaa ggccttttta ggaaggcgcg agcgccttct
     6181 caattgaaaa acctctctca ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac
     6241 gttgcacgtt cactaagata aagggtcttg caaagcaaga caaaatagaa aaaacttcct
     6301 ctcaccaaaa ggttcgttcc tgttcgttat ctctttattg atttgccaga ccggcaacaa
     6361 gttgcctttt atcaccaaaa agcgacaagg acaagggtaa atggcacgct gcgcgccctt
     6421 gtccttgtcg cttttcgttg attcattctg gccaacaaat caataaagaa agaactaacg
     6481 aaaggaagta aaacctttcc taagaggagg cagaatcaat catgcgaaag aaatttgatt
     6541 attggggtgt tcccttcagc gaattattcc caaactatca tgcaccacat actgtcgaat
     6601 gcgattgtgg agaaagagcg aaatgtatca aaagctatcg tttgtatcaa tgtccaacat
     6661 gcgggaaaaa atataccttg tcttatggc
//
LOCUS       DACEPD010000117         6466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.124, whole genome shotgun sequence.
ACCESSION   DACEPD010000117 DACEPD010000000
VERSION     DACEPD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.124"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(109..564)
                     /locus_tag="IW797_002504"
     CDS             complement(109..564)
                     /locus_tag="IW797_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225256.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IW797_002505"
     CDS             complement(718..1926)
                     /locus_tag="IW797_002505"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ1225257.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IW797_002506"
     CDS             complement(2038..2370)
                     /locus_tag="IW797_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ1225258.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IW797_002507"
     CDS             2518..3393
                     /locus_tag="IW797_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ1225259.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IW797_002508"
     CDS             complement(3475..4056)
                     /locus_tag="IW797_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ1225260.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IW797_002509"
     CDS             complement(4062..5321)
                     /locus_tag="IW797_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ1225261.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IW797_002510"
     CDS             complement(5323..6369)
                     /locus_tag="IW797_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225262.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1803 a         1337 c         1102 g         2224 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccg ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaa
//
LOCUS       DACEPD010000118         6122 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.125, whole genome shotgun sequence.
ACCESSION   DACEPD010000118 DACEPD010000000
VERSION     DACEPD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.125"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="IW797_002511"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="IW797_002511"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="IW797_002512"
     CDS             complement(580..1353)
                     /locus_tag="IW797_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1225263.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="IW797_002513"
     CDS             1497..1847
                     /locus_tag="IW797_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ1225264.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="IW797_002514"
     CDS             1828..2544
                     /locus_tag="IW797_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1225265.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="IW797_002515"
     CDS             2544..3992
                     /locus_tag="IW797_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1225266.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="IW797_002516"
     CDS             complement(4062..4571)
                     /locus_tag="IW797_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225267.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="IW797_002517"
     CDS             complement(4561..5286)
                     /locus_tag="IW797_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225268.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5544..>6122)
                     /locus_tag="IW797_002518"
     CDS             complement(5544..>6122)
                     /locus_tag="IW797_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1225269.1"
                     /translation="GRAFTNHRIQVMKTFKKGDRRSKHLKKYWRLLQKNAWELNGQHR
                     YWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVFLSAIRRQDVPEFVALLKEDYKE
                     LPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYRER
                     IFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         2020 a         1082 c         1238 g         1782 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt tgggctcttc gtcaatgagt gttggtgaag aaattcaata
     5521 gaataatcga gatactcaca cggctagaca gctatcttgt ctagccgtgt tgttgttctg
     5581 gctttagtca gttgggtagt gttcttagtt ttcttgaaat attttcctcg atataaaaag
     5641 attcgttctc ggtagttttg aaagtttcta aagccatagg caattcgttt tagtactttg
     5701 atatggttgt tcaaacattc tatagggccg ttggaatagg gaactcgcaa cgctcgtttg
     5761 atttctgttc gatatttctt gaaggtcgtg aacactggtt gatagtgctc tggcaactcc
     5821 ttgtagtctt cttttaggag cgcgacgaat tcaggaacat cttgtcttct gatagcggaa
     5881 agaaaaacct ggtagacttc gtagcctcgc tttaaggagt catcataata taatagccga
     5941 tcgactatct ccgcttctgt cagatgatct cgaaatgacg gacgccagta gcgatgctga
     6001 ccattcaatt cccacgcatt cttttgaagt agtctccagt atttctttaa gtgcttcgac
     6061 cgacgatccc cttttttaaa ggtcttcatt acttgaattc ggtggttggt aaaggcccga
     6121 cc
//
LOCUS       DACEPD010000119         5925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.126, whole genome shotgun sequence.
ACCESSION   DACEPD010000119 DACEPD010000000
VERSION     DACEPD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.126"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(76..1170)
                     /locus_tag="IW797_002519"
     CDS             complement(76..1170)
                     /locus_tag="IW797_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1225270.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="IW797_002520"
     CDS             1359..2120
                     /locus_tag="IW797_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ1225271.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IW797_002521"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IW797_002521"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ1225272.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="IW797_002522"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="IW797_002522"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ1225273.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4773)
                     /locus_tag="IW797_002523"
     CDS             complement(3760..4773)
                     /locus_tag="IW797_002523"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ1225274.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4938..5858
                     /locus_tag="IW797_002524"
     CDS             4938..5858
                     /locus_tag="IW797_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ1225275.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1181 c         1063 g         1875 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtc
//
LOCUS       DACEPD010000120         5904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.127, whole genome shotgun sequence.
ACCESSION   DACEPD010000120 DACEPD010000000
VERSION     DACEPD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.127"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(95..667)
                     /locus_tag="IW797_002525"
     CDS             complement(95..667)
                     /locus_tag="IW797_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1225276.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(674..1336)
                     /gene="rpe"
                     /locus_tag="IW797_002526"
     CDS             complement(674..1336)
                     /gene="rpe"
                     /locus_tag="IW797_002526"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ1225277.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1352..1867)
                     /locus_tag="IW797_002527"
     CDS             complement(1352..1867)
                     /locus_tag="IW797_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAQ1225278.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1869..2153)
                     /locus_tag="IW797_002528"
     CDS             complement(1869..2153)
                     /locus_tag="IW797_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ1225279.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2187..3515)
                     /locus_tag="IW797_002529"
     CDS             complement(2187..3515)
                     /locus_tag="IW797_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ1225280.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3529..3984)
                     /locus_tag="IW797_002530"
     CDS             complement(3529..3984)
                     /locus_tag="IW797_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ1225281.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3987..5852)
                     /locus_tag="IW797_002531"
     CDS             complement(3987..5852)
                     /locus_tag="IW797_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ1225282.1"
                     /translation="MAVSTKTIYQDIELLEDYVVNYQVTIERLPSLGIKLIGDRKNVE
                     HLISSVENYLEKDIYSPEKRRVEIIKEVLFGDKPVSLTHLSQSFMVSKTSLYNDLRII
                     NKIIGSGEVKLQSSAEGISFTGNEQAIQRAIKQLVFYYANDMESKTFFQVLNMLFDSK
                     VISVIHDLLFNDYSELTEKVSDYYVRSLISTLVIQCNRLLKGHHLTVEEEFLFNSIRY
                     METYIVANGLIEELKAKLNLPFYDADKEYLCRQLFAHRVTNNLKTSNEEYAMSVHKLI
                     ERMSEIEKIDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQNPLLENIKSQYSELFSIVW
                     YAMSILEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNIIIICQYGISSAQLIYNKVR
                     RFLPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIPLVRVSPLVSKTDYMNIME
                     AYTKYLIEEPSRFIQNELIPKAVKVPTISKFVQSSLVKTGVQVESKEECLDLMISELE
                     ANSLVTEEFRASIFSREKMGNTNLESGVALPHANPSMVIESNIFIVALKNAINWGDRK
                     VKLVIMVSLAEEDVSEIRGVYEELYQFIGQKEMVDRLLQVHDSEKLLRIFQQKGE"
BASE COUNT         1919 a         1147 c          836 g         2002 t
ORIGIN      
        1 aattattaag ttatgtctaa tcaaataaaa aaaccattct catatttgct gaataattag
       61 catttagaga atggtttttt atctttaaaa tctactactc aaaatctgca tgtcgagcaa
      121 acatcgtttc tgagctttca cctgttttat ccattaaaga taacactaaa ctatcaaagg
      181 tgagaaatgc taattgttca aaacttgaac ccataggttg tgaaaaagta tcttcttctc
      241 gttgattttc atctttggta atacctggta atacaagaac agaatctgct aattgaccaa
      301 tcgacgagtc ttttttcatg gtaactaaag caatgcttac accactttga tttgctttgg
      361 ccgctaaatt ttttaaacta cctgtttcac ctgagccaga acagataatt aataaatctc
      421 caggttgcga atgtggtgaa ctaatttcac cgacaaggct gactgaaaaa cctaaatgca
      481 ttaagcgatt cgtaaatgct tgcatggcaa ttccagagcg tcctgcacca gctagaaaga
      541 tgtgtttagc ttgaacaatt ttctcactaa attgttctaa ctctacagaa ttaatacgtt
      601 ttgagttatt ttgtaattct aagataatcg cttctaaata gtttttattt tggtctttat
      661 tggtcatgat cttttacttc acttcctcta atgattgatt tagtttatca atttgttctt
      721 gcggggtatt tttcccattg aagatgaaac cgcctgatac aaaaatatcc gcccctgctt
      781 tagaacaaac tttaatcgtt tgattatcaa tcttgccatc tacttcaata tcatacgtat
      841 aatcattttg tttgcgtatt acatctaact gttcgatttt aactactgtc tcttgaataa
      901 aatgcttatt ctctgttcct ggattgattg tcattactaa tacttgatcg actaaagata
      961 atactggttc aataaaactg actggtgttg caggattaaa agtaatccct acttttgctc
     1021 ctgcttcctt gactaactga atgaccttca ttgcgtgttt ggtagcttca atgtggaagg
     1081 taacaatatc tgctcctgct tctacaaatc ttgtgacaat tttttctggt gtatcaatca
     1141 tcaaatgaac atcaaacaca acgtctgtta gtggacgaat atctttaata acacttggtc
     1201 caaaagccat atctggtaca aaacagccat ccattacatc aatatgcaaa taatcaacat
     1261 tcttttcttt taagatttca aataactctt taacttctgc gataggtgca tttaacaatg
     1321 atggagcaat tgtcattctc tattcccccg tttattctcc cataatcatg agattgattg
     1381 ttctagttcg ctctcttagt tgatcccaat cgacaaaata gacacggccc atttctccta
     1441 ataaaagttt accttcttga acaattaaag tcattgagtt ggttccataa atagatgaac
     1501 gtaaatgtgc atcggtattt agcatgaccc atttttctgc agggtaattg ggatcagata
     1561 atcttgttcc aaaatcaatg tgttttggcc ccggactgtt gtattgattt tcactcgtca
     1621 tttttggaat aatcgtttcc attacattgt tgatgtctac atgtaaataa tcatcaccaa
     1681 aaaagtttgt gtcatgcatc gtttcttcaa aaaagagtga gcaagttgta tgcgggctcg
     1741 aaattactaa cattccattc tcaatacctg ctgtatcaac tgtatctaaa agttcttttc
     1801 gaatatcatg atacgaagga cgccctgaaa ctgtttcaat agtcaaagtg tcatgatgaa
     1861 aacgcatatt aaacacctaa tctttctttt acatctgcat aaatggcatc tgtaccaata
     1921 cctgttaata agcccaagcc attcgttact ttagctttaa ttacatcagg gacaattgtt
     1981 gtacttacta cagcatcata ttcatcaatt ttatttagct catcagcaat tttagattga
     2041 taaattttca cttgatctgt taatccttct tcttccaacc attttgataa ttttgctact
     2101 gcaattgtag aagtagcatg acctgctcca caaataacta ataatttttt catattgatc
     2161 tcttctttct tattttaatt ttatgattaa gctttcttca ctttgttcat gtagatagaa
     2221 gcacctacta aaacaactcc taatccacct attccaatta caccaaagta agatgctaat
     2281 aaaacaaaca accctactgg ccataatcca cataacaaca tagttactaa tcctgctgtc
     2341 cccacatcga aacttgctaa tgtaaacaca tcagtgataa ttggtgccaa ccatgttgcc
     2401 atatataatg ttgtaacaac atacaacgaa catcctacta atgatctcac aatatttcct
     2461 ttaaaataag gaatcattaa acagatggcg aaagcaaaga atgctaaatc tccaaaaggt
     2521 aatactttat ttcctggaag aattagtgct agcaataatg agattggtac aagcaataga
     2581 gaagttgaca taatagttgg atgacccaca gttaacgctg catccattcc aatgtttact
     2641 tggcgaccat ttaacttttt attcgtaaat tctttcgccg cttctgcaat tggcattaaa
     2701 ccttccataa acattgctac cattttaggc ataatcttca tcactgccgc agtcataatt
     2761 cctaagtttc caattcctgc tacgtcatag cctgccataa tacccactac aataccgata
     2821 attaggccaa taacaactgt gtcacctaaa acaccgaaac gttttgttaa tgtttctgca
     2881 tctgcatcta ttttattaaa tccaggaata cgatcaaaaa cccaatttac tggtttggca
     2941 aatagtactc ctgataatcc catcatatgt gtgatagaga ttcctggata gccgaaaaat
     3001 tcgctaacag ttttttgtgt acgatctgct aagaaccatt gtaaaattac tgcgacaatc
     3061 gcgccaatta agccaaacca aaagtttccc gtaacagatt gagtaatcat acctaggaaa
     3121 attgcgaacc aaaagttcca aatatcaaca ttcaatgttt ttgtccaacc caaaaccaca
     3181 aatatgaagt taagaattaa tgaaattgga atcattaaaa ttcccaatga tgtagagtaa
     3241 gccaaaggtc caccaacacc tacgccaata tctaggatat tcaggccacc accgtattgt
     3301 tcccccattt gttgaattgc tgttcctaaa ccatctgata ataaacttaa aactaatcct
     3361 aaaccaatag aaccgatacc aacagtaacg ccggaaacaa atgctttacc aggttttaat
     3421 ccaaataata tcccgattac aaaaataatc agtggtaaaa agacgtttga ccctaatcca
     3481 ataaaccatt caaaggcatt taatacaata tccatctctc tattcctcct aataaactcc
     3541 tgcagaatgt actaactcaa taaatgcttc tttcgtggta catgcaagta attcatttac
     3601 tttttcttca ttttgaaata ggctaactaa gttttgtagt gtatcaattt gttcatgagg
     3661 ttgtgacatt gccaacataa agactaaacg tacaggtatt tcatgagatt tatctgtcat
     3721 atctgaaaat accagtggtt cctttaatga ggcaaaacag atttgagagc tatttacatg
     3781 tgcggaatct gtatgtggga tagcaaaccc aatttgattt acttctaatc cagttggaaa
     3841 ttcttgttca cggttggtga taccttctaa gaaactatct ttcacgcaac cagcgttttt
     3901 taattgttca gacattactt gaaacaattc tttttgatct ttcacttcta attgaaataa
     3961 acataattct ttatctagat acatttttat tctccttttt gttgaaaaat tcttagtaat
     4021 ttttcgctat catgaacttg taacaagcga tcgaccattt ctttttgccc aataaattgg
     4081 taaagttctt catatactcc tctaatctca gatacatctt cttcagctaa actaaccata
     4141 atgactaatt tgacttttcg atcgccccaa ttaatcgcat tttttaatgc tacaataaaa
     4201 atgttggatt caatgaccat tgatggattc gcatgcggta aagcaacacc actttctaaa
     4261 ttagtattcc ccattttctc tctagagaag atagaagcac gaaactcttc agtaactaat
     4321 gaattggctt ctagttcgga aatcatcaaa tccaaacatt cttctttgct ttccacctgt
     4381 actcctgttt ttactaaaga tgattgaaca aatttagata tggtgggaac tttaaccgct
     4441 ttaggaatca gttcattttg aataaatcgc gaaggttctt ctattaaata tttagtataa
     4501 gcttccataa tattcatata atctgtttta cttaccaatg gagataccct tactaagggt
     4561 ataccacctg catcaaagga aagagaggaa ataatcaaat caacttcccc aatttcagct
     4621 aaatgaaact tatctaaagt tgatatttct acattatctt ttgctggtaa aaaccgtcga
     4681 actttattgt aaattaattg cgcagaagaa ataccgtatt ggcaaataat gatgatatta
     4741 tttgctttgg actgatcttc aatagcaatc tgaaaatgaa tcaaaattaa ggaaacttca
     4801 tcgtccgtta aagtcacatc aaattcttct tcaaggatgg acatggcgta ccatactatg
     4861 ctaaagagtt ctgaatattg agatttgata ttttctaata acggattttg aattttaata
     4921 ccttttttta aacgaaggac cattgctggc acatgataca ataaggattg atataatcga
     4981 tcattttgag tcaaatcaat tttttcaatc tcactcattc tttcaatcaa tttatgaacg
     5041 ctcatagcgt actcttcatt gctggttttt aaattatttg tcacacgatg tgcaaatagt
     5101 tgacgacata gatattcttt atctgcgtca taaaatggaa gattcaattt tgccttcaat
     5161 tcttcaatta aaccatttgc cacaatgtac gtttccatat agcgaatgct attgaagagg
     5221 aattcttctt caacagttaa atgatgacct ttcagtaaac ggttacattg gataactaaa
     5281 gtagaaatca atgaccgaac atagtaatca gaaacctttt cagtcagttc actgtaatca
     5341 ttgaatagca aatcatgaat gacactaata acttttgaat caaacaacat atttaaaact
     5401 tgaaaaaatg tttttgattc catgtcattc gcataataga atacaagttg cttgattgct
     5461 cgttgaattg cttgttcatt accagtaaaa ctaattcctt ctgcagatga ctgcaactta
     5521 acttctccac tgccaataat tttattgata attctcaggt cattgtataa cgatgtttta
     5581 ctaaccataa aactttgaga caaatgagtt aaagaaacag gcttatcacc aaacaaaact
     5641 tctttaatga tctcgacacg tcttttttct ggagaataaa tatctttttc aagataattt
     5701 tctaccgatg aaatcaaatg ttcaacgttt ttcctatcac ctatgagttt gatacccaaa
     5761 gaaggtaatc gctcaattgt tacttggtaa ttaactacat aatcttccaa caattcaata
     5821 tcttgataaa tggtcttggt cgacactgcc aatttatcac taaaaaaaga agcgggttta
     5881 aagtcttctt gtcgacttaa taat
//
LOCUS       DACEPD010000121         5874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.128, whole genome shotgun sequence.
ACCESSION   DACEPD010000121 DACEPD010000000
VERSION     DACEPD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.128"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            641..1375
                     /locus_tag="IW797_002532"
     CDS             641..1375
                     /locus_tag="IW797_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ1225283.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1391..2560
                     /locus_tag="IW797_002533"
     CDS             1391..2560
                     /locus_tag="IW797_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1225284.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2576..3571
                     /gene="lacD"
                     /locus_tag="IW797_002534"
     CDS             2576..3571
                     /gene="lacD"
                     /locus_tag="IW797_002534"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ1225285.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3650..4576
                     /locus_tag="IW797_002535"
     CDS             3650..4576
                     /locus_tag="IW797_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ1225286.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4595..5845
                     /locus_tag="IW797_002536"
     CDS             4595..5845
                     /locus_tag="IW797_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ1225287.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         1966 a          955 c         1203 g         1750 t
ORIGIN      
        1 gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg actaaacgaa
       61 gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt cagataaact
      121 ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct taagagagaa
      181 cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt tattctcata
      241 gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat ttgaaaccaa
      301 agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac tggtaagtag
      361 tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa aaaaagatac
      421 cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa agttggaaaa
      481 ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata ttaacaagac
      541 taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa taccactact
      601 atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata cctttcaatc
      661 aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa acagtatggt
      721 cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg ttagtcgcac
      781 gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc gacaacatgg
      841 aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg gcgcatatag
      901 ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat tggccttcaa
      961 gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag gtgagccttt
     1021 atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg agaaatccta
     1081 tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga aacctttata
     1141 taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg aattttatgc
     1201 cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat caccagtgct
     1261 ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta cattaagtgt
     1321 agcgcgggct gataaatttc attataagat caggcatctg cgttccacta actgaattgg
     1381 aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag cagaaaatac
     1441 tacccgagaa attcgacagc aaccggaact gtggcatgag acgtttgacc gttatcaaga
     1501 aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg gagagcgacc
     1561 aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata cactaattcc
     1621 gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg gttcgaccga
     1681 tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct tggtctcatt
     1741 tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca atcagctggt
     1801 caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg ctacacaaat
     1861 gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg acgctggttt
     1921 tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt ttgatcaagc
     1981 aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag aagaagtcat
     2041 ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca tcatttactt
     2101 aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc tcgagttaac
     2161 tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg gtcccaaatc
     2221 ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat atacgcagaa
     2281 atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac gcgttgttgg
     2341 aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg cagattcgtt
     2401 actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga tcgcattact
     2461 ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa cagtcaatcg
     2521 tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt gaagcatgtt
     2581 agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag aaggcattat
     2641 taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg ctttagggat
     2701 ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag aattgactcc
     2761 ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac agaagcgtgc
     2821 agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa ccgtacccgg
     2881 ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt taggtgcgga
     2941 tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca atcggcaaaa
     3001 gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc cattttattt
     3061 agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct atgccaaagc
     3121 aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt ataaagtaga
     3181 tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg caacagggga
     3241 gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg atgcaacaga
     3301 tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg agacattgcg
     3361 ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag caacttggaa
     3421 agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat ggctgacaga
     3481 acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg ccatcccatg
     3541 gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta gtgataagag
     3601 atatgtgtga ttataattta tcattattga aactgggagg atattttata tgaataaaag
     3661 attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat taaagcaggc
     3721 aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta gctgtcctta
     3781 catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg atttaatttt
     3841 gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa ctctaaaaga
     3901 gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa atttagaacc
     3961 tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg gtagaatcgt
     4021 ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt gtttaactgg
     4081 aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat tatccaaatc
     4141 aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg atgaaccaat
     4201 tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt tattagttcc
     4261 tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg ttgattatat
     4321 aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa cggctaatgg
     4381 aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag catcaaaagc
     4441 atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc ttccagaaaa
     4501 tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta tgttaggaaa
     4561 atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc aaaaaatagc
     4621 tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg ctgcagttat
     4681 gccattaatc attattggtt ccttttttat gttattagca aatttcccga tagaaggatt
     4741 tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat cagattcaac
     4801 atttggtatt attggattag ctgttaccta ctctttggga cacaatctag caaagactta
     4861 tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt tacttacacc
     4921 tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta cctcgggtct
     4981 ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt ttgtacttaa
     5041 aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag ctttcgttgc
     5101 tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa tttgttataa
     5161 acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac cactatcttt
     5221 actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt tcttttggat
     5281 gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat tattgcaaaa
     5341 ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca ttataactta
     5401 tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg cacttgccat
     5461 tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt tatcaatcgc
     5521 tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta tattaaatat
     5581 aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta cttatttttc
     5641 aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga caactccacc
     5701 gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga ttcaagtaat
     5761 aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg ataatgccaa
     5821 gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta gtta
//
LOCUS       DACEPD010000122         5845 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.129, whole genome shotgun sequence.
ACCESSION   DACEPD010000122 DACEPD010000000
VERSION     DACEPD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.129"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(437..610)
                     /locus_tag="IW797_002537"
     CDS             complement(437..610)
                     /locus_tag="IW797_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HAQ1225288.1"
                     /translation="MNRYVHGTVQKKNRKINGKLLVNPNGYEWLRAKGSYPVPKYCNY
                     SETLMEWYQLIIT"
     gene            complement(953..1642)
                     /locus_tag="IW797_002538"
     CDS             complement(953..1642)
                     /locus_tag="IW797_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ1225289.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFSHTRVGKNGKTFKMHKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYIGHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(1705..2469)
                     /locus_tag="IW797_002539"
     CDS             complement(1705..2469)
                     /locus_tag="IW797_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ1225290.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIVKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            complement(2497..3195)
                     /locus_tag="IW797_002540"
     CDS             complement(2497..3195)
                     /locus_tag="IW797_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ1225291.1"
                     /translation="MARTQKQKAAQTHVVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTIAANIAVVFAKSGQRVLLVDADLRKPVVYETFQLNNAS
                     GLSTALSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYRN"
     gene            complement(3207..3986)
                     /locus_tag="IW797_002541"
     CDS             complement(3207..3986)
                     /locus_tag="IW797_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ1225292.1"
                     /translation="MEETISLKEIFGTLRKHLATIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSENSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4002..4946)
                     /locus_tag="IW797_002542"
     CDS             complement(4002..4946)
                     /locus_tag="IW797_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ1225293.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4992..5771)
                     /locus_tag="IW797_002543"
     CDS             complement(4992..5771)
                     /locus_tag="IW797_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225294.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKERVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNINY"
BASE COUNT         1567 a         1206 c          973 g         2099 t
ORIGIN      
        1 atcttaatta tctatccatt tcatcctacg cattcttgcg taaatacaat tcactctgtt
       61 gagctgatat aaatcattgg tggtacaaag ataaaatgat aactgtttct atacctccaa
      121 aaattccagt aaatatatct atagatagtt tcaaaaaaaa ttgtcttagt ttcataatat
      181 ttaagatagt ggtacatact ttataacaac gtacttttcc aaatattatt ttccgttatg
      241 aacatccaat atattacaaa gtttaaaagt ttagatgaac tataatcttc atttctgcac
      301 agtggataat aaattcttat agataaaaaa tagcctcttc taaagtcaca aataccttac
      361 tcccacattt taagtaaaca ctctgcagca ttctctttgt tataaaatga tagatattcc
      421 tcaattttat ctcttattat gttattatca gttgatacca ttccattaat gtttcagaat
      481 aattacaata ttttgggacc ggataactcc cttttgctcg taaccactca tatccgttag
      541 gattcactaa aagtttaccg tttatttttc gattcttttt ttgaaccgtc ccatgaacat
      601 accgattcaa caagctaaat tataaaaatt ggtgtgctgt cattattttt tggaaaattt
      661 attaatggct atcaatatac ttttgtatca tactaaatag ctcgtcagtt cctatatcgg
      721 atataccaat attaaagcat ttagatcatt atatttaaga gtggtatcat cccaaaattt
      781 cgaatatttt cttttatata agaataatga tactgaattg ccccattcac ctttcttttt
      841 ttagattttc tacaagtgat tcaaaccctc cataattaca gttattactt ttgaaacgat
      901 aatatgaaca tgtgtaatct tttttattaa caatccatca ttctatccct aactaatatg
      961 ctccattggg cttgacaata acataaaacg tttttaaaat caacctcaag tcgtacatta
     1021 aacttcgatg accgatatac tctaaatcca actcaaccat tttatggaaa ctaacattat
     1081 ttctatcact aacttgccac aaacctgaac aaccaggttt tacatacaag cgttgaagat
     1141 catatctcgt gtactcagct acttccctct ccaaaggtgg ccgtggacca attagactca
     1201 tatcgccttt taaaacattc catagctgtg gcaattcatc aatgcttttg gcacggataa
     1261 ttttacctat tttagtgaca cgcggatctt ctttcatctt gaacattgca ccttctattt
     1321 cattttgatc aagtaattct tccaattttt cttcagcatc taaacacatc gaacggaatt
     1381 tatgcatttt gaatgttttt ccatttttac caacacgtgt atggctaaaa aatattggac
     1441 ttcctggctc ttcttttctg attcgataag ctacatagaa catcaaagga gaagctaaaa
     1501 tcaaaccaat aatgcttcct acaatatcaa tcattctttt aaaaaataaa tagatatatc
     1561 gtttttcaac tttttcttta tccaaccaaa cttgattagg atgtacttca ctaatacgag
     1621 ataataaatc ttcactttcc atactttcag tccctttcac tatcttcact ctctcctcat
     1681 ttgtttttat ctacgaataa agaactaaaa taaaccaaac ttcttctttt ttacctcttc
     1741 atagattgga taactgatct catccccgtt gatcagatcc tttgcgactt gtttcatctg
     1801 tactactttt tccttaccaa agtccttgac aatctgctca tagcactctt ttaagtaaaa
     1861 ggtgcgctta gagaccccgt gtgcatcaga agcagccatc tgcacgagat tatgtttgac
     1921 cattgttttg gctgttttct ggatgtcttt gccaaataca ccaacataac ttggcgcagt
     1981 aatttgggcc aaacatccca tatctaggaa cggaagcaaa tgatttgggt cttctcgaaa
     2041 atgcgcgttg cgttctggat ggacgatcac tggtgtcttt cctagttcta acaactcaaa
     2101 aaaaagccat tctgcatata acggcacttc cagtgtcgga aattcgatca gaagatatgt
     2161 atcatctaaa tctgtaaata aaatttcatc tcggtggatg tcttcgatga gcgttccggt
     2221 aatacgtact tcttgtcctt ccaataacgt caaaggcaac tgtcttttct ctaattcggc
     2281 ttgtaacgaa gcgactagag aaatgacttg tgatttttca ttgctgtatt ttccattatt
     2341 gtggtgtggt gtgcataaaa tatgcgtgat gccttgctga atcgcttttt cagccattgc
     2401 aatacttgct tctaagtttt ctgccccgtc atcgattcct ggtaggatat ggcaatgtag
     2461 atcgatcatt tctcttgtct cctctcttct tattccttaa tttctgtagt aataaccagc
     2521 atctttgcta tgttctgctc cgttgtatac aacacctaag actcgtgctt gtaccatctc
     2581 tagcagttct tttgctttag tcaaggattc tttacgtgat gtattttcac gtacgaccaa
     2641 taaagtgcca tccgtttttg aagacatgat ctgtgcatct gttacggcta ctactggtgg
     2701 catgtcgaag atcactacat caaatagttg tcttgcttct gctaaaatct gatccattct
     2761 tggcgagctt aataactctg atggatttgg tggtttaggt ccacttggca gaatcgacaa
     2821 attctcgact ggtgttcgtt ggatctcatc tgctacactt cctgatgaac taagtgctgt
     2881 acttaatcca ctggcattgt tcagctggaa tgtctcatag acgacgggtt tacgtaaatc
     2941 tgcatcgact agtagaactc gttgaccgga tttagcaaaa acgactgcaa tgtttgctgc
     3001 aatggtggat tttccttctc caggacctga tgaagtcacg acaatcgttt tgatttgctg
     3061 accagctgcg gaggcaaact ggatattcgt ccggatcgta cgatattgtt cggcgattgg
     3121 tgaagatggg tttgttaatg tgatcaaact gactacatga gtttgtgctg ctttttgttt
     3181 ctgtgttcgt gccatttttc ttcctcctag actcttgagc ggcttctgcg tgatggagct
     3241 gttttatcgt ttgtagcttt tggtcgtgca ggagctgatc cttttttacg gataccagcg
     3301 ttcaattctt tcgctgacat ttctggaact gttcctaaga ttgggaatcc taatgtatcc
     3361 gtcacatatt tttcatcttt cactgtccga tcaagtaatt cgaataagaa tgccaaacct
     3421 acgcctacca tcattcctaa tactaagcca atcactagat tcaatttatt atttggcgaa
     3481 acaggtgtcg tattggcaac tgcacctgag atgatcgaga ttttatcgac attcatcacg
     3541 tcttttgcgt tctcttggaa aacttgtgaa gttgtattag cgatctgttg agcagtcact
     3601 gcattcgtgc ttattgcctg gatcgagaac atttgtgagt tttctgactg attgacggag
     3661 atcgcatctt tgatttgtcc agcggtcatt ttcacattgt cttctgtttc tagacgatct
     3721 ttgacttcat ttaatactag atcactaacg atcatatctt tatacgtatt gatcatttgc
     3781 aggttcgtat tgacatcatt gacattcgct gtgtctgatt gcggcagtgt cacgatcaat
     3841 tgtgcttgtg aactgtattt tggtgtcaag atgaaaaatg ttacgattcc tgagatggcc
     3901 aagccagcaa acattgagat gatgattgtt gccaagtgtt tgcgtaatgt tccaaaaatt
     3961 tctttcaagc tgattgtctc ttccattttt tctccacttt cctatttatt ctctagattc
     4021 atttgatctt ttaattcttt tttcacacga tcaagttctt gatcgctgac tttttgatag
     4081 gagatattat cttgcatgaa gccttctcct tgcagctgat cttgcttgat cgtaccaaat
     4141 gcctcccgat aatctaaagc gatttttttc atttcatcaa agctcatgtc tgttttcata
     4201 ttcgattcta gagcattcaa aacggattga tagttgctga caccgtttag actcaatact
     4261 tttttcgcta caccttctac gatctttctt tgacgctctt gtctaccata gtcaccattt
     4321 ggatcttcat aacgcatacg agaataagat aatgcctgat ccccgttcaa tgtgatttta
     4381 ccgattgtaa agtcatagcc atcttgtgag aaagtgaggt cattattgac ttcgatacca
     4441 ccaactgcat caacaagttc ttttagccct ttcatattga tagagacata gtgatcgatt
     4501 gggatgtcta catatttttg tacggtatcc atcgccatgg cagctccgcc gaatgcgtaa
     4561 gcgtggttga tcttgtcttt tgtgtcacga cctacgatgt cgacatacgt atctcgagga
     4621 atgctgacga tcgttgtctg tttgtcttca ggactgactg tggcaaccat catcgtatct
     4681 gatcttcctt gatcgacccg acctaaatct ccagtatcaa tccccatcaa tagaacagag
     4741 aaagattctt gtttgtccag atcaactggt ttcgttcgtt ctccttgggt tcgttccact
     4801 gattcgtaag ttttctcaat agaatgtgag agatccagat ataattttgc tcctactcct
     4861 acaattgcta atactaatac gataagaact gctaaaataa tcagaaatat ttttttccct
     4921 cttgattttc ttcttcgtct tgccatagtt tcctccgttt gctaaccttg cttgaatcat
     4981 cgctttaact gtcaatagtt tatattgcta tagcccattt gctgagcaac agtttgtgct
     5041 tttgttgcgt atgctacgct tattttttca ttccggatct gctctatttg actgagcgcc
     5101 gtcaaatatt gatcgtaagt agcggtggca gtcacttgac catcctttaa catcgtatcg
     5161 aataactgat ctagttgcgt gacttgcgcg atctgggtat cagcgaatcc tagatattgc
     5221 ataacaaccg ttttccaagg actatcttta aaaaagctca tattttcacg tacacgagcg
     5281 acatctgcac ttgtcaccct ttctttgatg atgacatcgt ctacagcttg ctgccagttt
     5341 gatacatttt tagtaaataa atcgttgaca gctgtctgga tcttcagttt tgattctgca
     5401 ctatctaact gtgtcacgat tgctttcttc tcatcttgga ttgcaacagc ttctttaggc
     5461 aattctgatt ctttgatgtc gtaatcatct gctgaaagtt tgacagagga tagcttattt
     5521 gacaaacttg tgattgtttc ttctgttatt tcagggttta gaaaaatttt ttcttttgta
     5581 ctatacagct tgtctacttc ttttgataaa gaagtcaagg tgtctttttg attggtgatg
     5641 gcagattctg cataattgat ccgttgctga tgttgccgct gattattgat atggtatcca
     5701 aacaaacaga tcgcaatagc acctgcacct accgccacag cagtcaatga cttgacgaat
     5761 ggcttttcca tagattcgct cctaagtatt gatgatagat aaattaagat ataattcact
     5821 tacataaaaa tgacagtttt ttatt
//
LOCUS       DACEPD010000123         5748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.130, whole genome shotgun sequence.
ACCESSION   DACEPD010000123 DACEPD010000000
VERSION     DACEPD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.130"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(43..2193)
                     /locus_tag="IW797_002544"
                     /pseudo
     CDS             complement(43..2193)
                     /locus_tag="IW797_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2339..2914
                     /locus_tag="IW797_002545"
     CDS             2339..2914
                     /locus_tag="IW797_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1225295.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3128..3823
                     /gene="vanR"
                     /locus_tag="IW797_002546"
     CDS             3128..3823
                     /gene="vanR"
                     /locus_tag="IW797_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1225296.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3801..4955
                     /locus_tag="IW797_002547"
     CDS             3801..4955
                     /locus_tag="IW797_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1225297.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            complement(5053..>5748)
                     /locus_tag="IW797_002548"
     CDS             complement(5053..>5748)
                     /locus_tag="IW797_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1225298.1"
                     /translation="QLPFLKRYFSHFSREIREAVEWIVIDMYAPYVSLVKKLFPKAQL
                     IIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQKHYQLGKQLKRYWKLLQKDERKLDYT
                     RRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQLYQDFLYAVKERDYVSFEELLTNNI
                     MLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKL
                     RIMIRHGNALIFN"
BASE COUNT         1777 a         1068 c         1122 g         1781 t
ORIGIN      
        1 tagtttgaaa tgcaattttc ttctttcagc tttttatttc ccacttgtaa atcggtagat
       61 ggtggctcct tttccagttc cataatgtgg atttgcatct gcatgtagtg atgaaacacc
      121 tagctgcatt ctcataccat ctgacgaaga tgttgtaccg tcgccccaat agaaaggcaa
      181 ttgtaattta tgatgaaagt ttactaatat ggcttgggct ttattcatgg catcttcata
      241 catgcgccat tgagatacat tggctagttg cttatatgta agtccgggtg tggcttcggc
      301 catcttgctc aagccaatat tcattcccat tcctaaaagg gcagccatga taatgattgt
      361 ttcttcctta tctggttttc gattattgga agcatgagtg aattgctcat gaaatcctgt
      421 tatatgggcc acatccatga gtaaatcagt taattttatt cttggtagca tctgataaag
      481 gcttgcacta aatttttttg cttcttctgg aacatctttt tctaagcgtg caagtgatag
      541 ctttcctttt tcaagagaaa ccccatctaa cttattggaa ttggcagcta accactttaa
      601 cctttcatta aagctgctgg ttctctccgt tatataatct tcgaatgata aactaactga
      661 taatctcgta ttccccttcg attgattcca tgtatcttcc gaaaacaaat attcctcaaa
      721 atccctatat tgtctgctgc caacaatgga aacatctcct gcccgaacat gctcccgaag
      781 ttctgttaaa acagccattt catagtaatg acgattaatt gttgtaccat catcctcgta
      841 taaatgtctt ttccatcgtt ttgaaataaa atccacaggt gagtcatcag gcacttttcg
      901 ctttccagat tcgttcattc ctcggataat ctcaacagct tgtaaaagtg gctcatttgc
      961 ctttgtagaa tgaaattcca atactcttaa tagcgttggc gtatattttc ttagtgaata
     1021 aaaccgtttt tgcagtaagt ctaaataatc atagtcggca ggacgtgcaa gttcctgagc
     1081 ctcttctact gaagagacaa aggtattcca ttcaataacc gattctaaaa ccttaaaaac
     1141 gtctaatttt tcctctcttg ctttaattaa tgcttgtccg atgttcgtaa agtgtataac
     1201 tttctcattt agctttttac cgttttgttt ctggatttcc tcttgagcct tacgaccttt
     1261 tgataacaaa ctaagtattt gcctatcatg aatttcaaac gctttatccg ttagctcctg
     1321 agtaagttgt aataaataga tggttaatat cgaataacgt ttattttctt gaaagtcacg
     1381 gaatgcatac ggctcgtatc ttgagcctaa gcgagacagc tgcaacaggc ggttacggtg
     1441 caaatgacta atttgcactg tttctaaatc cattcctcgt atgtattcga gtcgttctat
     1501 tatttttaga aaagtttcgg gtgaaggatg acccggtggc tcttttaacc aacccaatat
     1561 cgttttattg gattcggatg gatgctgcga ggtaataatc ccttcaagct tttctttttg
     1621 ctcatttgtt agagatttac taaccgtatt aaatagcttc ttttcagcca ttgcccttgc
     1681 ttcccacacc attctttcaa gtgtagtgat agcaggcagt ataattttgt tttttcttag
     1741 aaaatctatg cattcatgca gtagatgaat ggcatcacca ttttccaaag ctaattgatg
     1801 aaggtactta aatgtcattc gatattcact cagggtaaaa gttacaaagt cgtattcact
     1861 tcgaatttct ttcaaatgat cccaaagtgt attttccctt tgaggataat gatcaagcga
     1921 ggatggacta acaccaatct gtttcgatat atattgtatg accgaatctg ggatgctttt
     1981 gatatgagtg tatggccaac cgggataccg aagaacagct aattgaacag caaatcctaa
     2041 acggttttct tccctccttc gcttattaac tatttctaaa tcccgtttgg aaaaagtgaa
     2101 gtaggtcccc agtatccatt catcttcagg gatttgcata aaagcctgtc tctgttccgg
     2161 tgtaagcaat tctctacctc tcgcaatttt cattcagtat cattccattt ctgtattttc
     2221 aatttattag ttcaattata tatcaataga gtgtactcta ttgatacaaa tgtagtagac
     2281 tgataaaatc atagttaaga gcgtctcata agacttgtct caaaaatgag gtgatatttt
     2341 gcggaaaatc ggttatattc gtgtcagttc gactaaccag aatccttcaa gacaatttca
     2401 gcagttgaac gagatcggaa tggatattat atatgaagag aaagtttcag gagcaacaaa
     2461 ggatcgcgag caacttcaaa aagtgttaga cgatttacag gaagatgaca tcatttatgt
     2521 tacagactta actcgaatca ctcgtagtac acaagatcta tttgaattaa tcgataacat
     2581 acgagataaa aaggcaagtt taaaatcact aaaagataca tggcttgatt tatcagaaga
     2641 taatccatac agccaattct taattactgt aatggctggt gttaaccaat tagagcgaga
     2701 tcttattcgg atgagacaac gtgaagggat tgaattggct aagaaagaag gaaagtttaa
     2761 aggtcgatta aagaagtatc ataaaaatca cgcaggaatg aattatgcgg taaagctata
     2821 taaagaagga aatatgactg taaatcaaat ttgtgaaatt actaatgtat ctagggcttc
     2881 attatacagg aaattatcag aagtgaataa ttagccattc tgtattccgc taatgggcaa
     2941 tatttttaaa gaagaaaagg aaactataaa atattaacag cctcctagcg atgccgaaaa
     3001 gccctttgat aaaaaaagaa tcatcatctt aagaaattct tagtcattta ttatgtaaat
     3061 gcttataaat tcggccctat aatctgataa attattaagg gcaaacttat gtgaaagggt
     3121 gataactatg agcgataaaa tacttattgt ggatgatgaa catgaaattg ccgatttggt
     3181 tgaattatac ttaaaaaacg agaattatac ggttttcaaa tactataccg ccaaagaagc
     3241 attggaatgt atagacaagt ctgagattga ccttgccata ttggacatca tgcttcccgg
     3301 cacaagcggc cttactatct gtcaaaaaat aagggacaag cacacctatc cgattatcat
     3361 gctgaccggg aaagatacag aggtagataa aattacaggg ttaacaatcg gcgcggatga
     3421 ttatataacg aagccctttc gcccactgga gttaattgct cgggtaaagg cccagttgcg
     3481 ccgatacaaa aaattcagtg gagtaaagga gcagaacgaa aatgttatcg tccactccgg
     3541 ccttgtcatt aatgttaaca cccatgagtg ttatctgaac gagaagcagt tatcccttac
     3601 tcccaccgag ttttcaatac tgcgaatcct ctgtgaaaac aaggggaatg tggttagctc
     3661 cgagctgcta tttcatgaga tatggggcga cgaatatttc agcaagagca acaacaccat
     3721 caccgtgcat atccggcatt tgcgcgaaaa aatgaacgac accattgata atccgaaata
     3781 tataaaaacg gtatgggggg ttggttataa aattgaaaaa taaaaaaaac gactattcca
     3841 aactagaacg aaaactttac atgtatatcg ttgcaattgt tgtggtagca attgtattcg
     3901 tgttgtatat tcgttcaatg atccgaggga aacttgggga ttggatctta agtattttgg
     3961 aaaacaaata tgacttaaat cacctggacg cgatgaaatt atatcaatat tccatacgga
     4021 acaatataga tatctttatt tatgtggcga ttgtcattag tattcttatt ctatgtcgcg
     4081 tcatgctttc aaaattcgca aaatactttg acgagataaa taccggcatt gatgtactta
     4141 ttcagaacga agataaacaa attgagcttt ctgcggaaat ggatgttatg gaacaaaagc
     4201 tcaacacatt aaaacggact ctggaaaagc gagagcagga tgcaaagctg gccgaacaaa
     4261 gaaaaaatga cgttgttatg tacttggcgc acgatattaa aacgcccctt acatccatta
     4321 tcggttattt gagcctgctt gacgaggctc cagacatgcc ggtagatcaa aaggcaaagt
     4381 atgtgcatat cacgttggac aaagcgtatc gactcgaaca gctaatcgac gagttttttg
     4441 agattacacg gtataaccta caaacgataa cgctaacaaa aacgcacata gacctatact
     4501 atatgctggt gcagatgacc gatgaatttt atcctcagct ttccgcacat ggaaaacagg
     4561 cggttattca cgcccccgag gatctgaccg tgtccggcga ccctgataaa ctcgcgagag
     4621 tctttaacaa cattttgaaa aacgccgctg catacagtga ggataacagc atcattgaca
     4681 ttaccgcggg cctctccggg gatgtggtgt caatcgaatt caagaacact ggaagcatcc
     4741 caaaagataa gctagctgcc atatttgaaa agttctatag gctggacaat gctcgttctt
     4801 ccgatacggg tggcgcggga cttggattgg cgattgcaaa agaaattatt gttcagcatg
     4861 gagggcagat ttacgcggaa agcaatgata actatacgac gtttagggta gagcttccag
     4921 cgatgccaga cttggttgat aaaaggaggt cctaagagat gtatataatt ttggctcttt
     4981 gtcaaatgat gttggtaaag agaaatctta gaataattgg agatcaagtg gatcagttcc
     5041 tcttgatctt ttttagttga aaatcaatgc gttgccatga cgaatcatga tgcgcaattt
     5101 gaaattataa aagctacgaa agccatatgc atttcgtttt aacactttga tatggttgtt
     5161 cagacattca agtgggccat ttgaataact ttgttgtaaa gcatttttga tttgtggcag
     5221 aaatttctgg aacgttctca aggtcgtctg atagccttcg ggaagcatga tattattggt
     5281 aagtagttct tcaaacgata cgtagtctcg ttcctttaca gcgtaaagaa agtcttgata
     5341 taactgatag cctacacgca aagcagggct ccctttcagt aagcgatcca caatatcggt
     5401 ctctgttaaa tgagctttga agccaggtcg ccaaaggcgt cgagtataat caagcttgcg
     5461 ttcatctttt tgaagaagtt tccagtatct ttttaattgc ttacctaatt gatagtgttt
     5521 ttgttctttg ctttttaata gttggttcgt ttctttgatt cgatgattgc gaaaggtacg
     5581 accaatatgt tggacgatgt gaaaccggtc aataatcagc tgtgcttttg gaaacagctt
     5641 tttaacgagc gatacataag gagcgtacat atcaatcacg atccattcga ccgcttcacg
     5701 aatttctcgt gaaaaatgac taaaataacg ttttaaaaaa ggcagttg
//
LOCUS       DACEPD010000124         5682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.131, whole genome shotgun sequence.
ACCESSION   DACEPD010000124 DACEPD010000000
VERSION     DACEPD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.131"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            15..2180
                     /gene="acm"
                     /locus_tag="IW797_002549"
     CDS             15..2180
                     /gene="acm"
                     /locus_tag="IW797_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ1225299.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2452..2937)
                     /locus_tag="IW797_002550"
     CDS             complement(2452..2937)
                     /locus_tag="IW797_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ1225300.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3417..4058
                     /locus_tag="IW797_002551"
     CDS             3417..4058
                     /locus_tag="IW797_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ1225301.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4077..4712
                     /locus_tag="IW797_002552"
     CDS             4077..4712
                     /locus_tag="IW797_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ1225302.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4730..5545
                     /locus_tag="IW797_002553"
     CDS             4730..5545
                     /locus_tag="IW797_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ1225303.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         2006 a          919 c         1166 g         1591 t
ORIGIN      
        1 gggagataga aaaaatgaaa aagtgtagaa atattatttt atcaatgtta tttattatta
       61 ctaatataac atcactgatt cctgttcatg tctatgcgga tgcaggcaga gatatcagca
      121 gtaatgtcac ttcgttgacc gtggacccaa cgaatattac agatggagga aacataaaag
      181 taaaattctc gtttgacgaa aaaaaacaaa atatccaacc aggtgattat ctttggatca
      241 attggccaag tgaaggaaat attcgcggtg aaggattcca aaaagaaatc ccattaatga
      301 ttgaaaataa aaatgttgga actttaacgg tcagaaaaga ttctgcgcag gttgtgttta
      361 atgaaaatat taaaaaccta gactcagtag aaggttgggg gcaatttgag atacaggcca
      421 gaaacgtaac cgataccggt gaagaaaata cgggaccatt caccgtgacg agcggtgata
      481 aaacagctac tgtaaatgtg acaaagccag cttctggttc ttcttctagc gttttttatt
      541 ataaaacagg agacatgcta cctgaagata ctaagcacat tcgatggttt ttgaatatta
      601 acaataacgg cacttatgtt gaacaaccag ttaaaatcag tgatgaaatc caaagtggac
      661 aaagattaga tccaagtacg tttgaaatta atcaaataca cttaggtgaa cagaaggttt
      721 atagaggaga agagggaatc caacaatttt tacaagattt ccccagcgct acatttaatt
      781 tttcagtaac tgataattac attgaaataa ctataccaaa aaactttgtc aatcttagga
      841 aaataatggt gagttataag acgatcatag aaaacccaga acaaataaat tttgagaatc
      901 attcggaagc ttggtttaaa gaatttaata aacctgctgt tgatggagaa agtttcaatc
      961 atacagttaa aaatatttca gcttctggtg gagtcaacgg tacagttaga ggcgaattga
     1021 agattttcaa atatatcaat gacacagaga ttggtatccc aaatgtgact ttcgaattaa
     1081 gacgagcaga tgaacagcct attcaaggac agtctagtat cctgttgact tcgaatgaac
     1141 agggagaagc tagtatcaaa ggcctgcaag taggagacta tgttgtaaaa gaaaaagaag
     1201 cacccaattg gattgatttt gatccactaa gttcaaatga actcaaattc tccataaacg
     1261 aaaatgatac agagggagtc agtttaccta tttataacaa gaaaaaggtt acgaacatca
     1321 cagcaactaa aatttggaac ggtggaacca ctccaagacc gagtatttat tttaaattat
     1381 tcagagcaag tacaaacaat tgggaaccag ttcctgatgc agaaacaaaa cgattagaca
     1441 atggaataac ttcagtgact tgggaagata tccaacagta tgatgatagt ggaaacgaat
     1501 atacgtttaa agtgcaagaa gtagatcaaa acggaaatga ttatgtccct tcaggttatc
     1561 aaaaaattga aaacgggcta gtcgttacaa atgagaataa agaagtcatt tcagtctcag
     1621 gacaaaaaac atgggaagac aatgagaacc aagacggcaa acgaccgacg acgatcacag
     1681 taaatctatt agccgacggt caaccgatcc aacacaaaga agtaagtgaa aaagatgatt
     1741 ggcgctatcg tttcacaaat ctgccaaaat acagagacgg gcaagaaatc atctatactg
     1801 taacggaaga caacgtgccc gaatacagta cgacgattga cgggtataac atcaaaaaca
     1861 gttatacacc ggaaaaaaca aatatatcta taccaggtag ccatataaca ccgtggaaaa
     1921 attttccaga gaagacagag agagagaaaa ttagaaatct attctctcaa aaattgcaaa
     1981 tatttgctca tacaggagaa tccagcgaac aaagaagcaa ttatattagt aagagagctg
     2041 tacctaaaca gataacaaat aaatcaaaaa gcattcttcc taaaacaagt agtaaaaaat
     2101 ctagttttgc tgtaatcatt ggtttattga tagtaactat ctgtgctggg atattcttac
     2161 aaaaatataa aaaagtttag ttagaatata atattttttg ttaaatatga ctgaaatcaa
     2221 aagaataaaa ttccgattta taatcgaaaa aagaagcatt tttatatatg cttctttttt
     2281 cgtagaatca ataaatatgg atttggctaa ctatggaaga atactcgtat tagttttcag
     2341 cgttcatact agttgttaca ttgtttgatt ggccaaaaga atttctgata tattttgtgc
     2401 taaataaaga aaaagacgct tgaattggga aataagaaaa ctactctatc attacaaatt
     2461 ttcataaact ggcagatgtt ctaagtaaac aagtatcttt tcaaagtcgg aaaaatccat
     2521 aatcaacgta gtggtattga tatttggatg aaagcctacg gtatcttgac gatcaatagc
     2581 agcatcgatc acgacttgga tttttttctc tttgtcgtgc atcaatgcta atggagtcac
     2641 tgtacctgct ggaacgccta ggaggtcagg aagctgttct tcagaaacaa aagataagcg
     2701 cttttctttt aattgttcag ctagttgttt taaatcggcg tgcttttcat caggcaagat
     2761 gacaagatag atttgttttc cttttcttga tttcaataat agatttttta cttgcggacc
     2821 aggtagggaa aaagggacat ttttgactga cgtaatcgca ggatgatcga ctttttgaaa
     2881 agagataccc agttttttta gtagttcata agcttcttgt tcggttgcac gactcatcgc
     2941 gcatcaccct ccttttttta ttagtgtatc ataaaaaaga gttgacaaat gaaaaataat
     3001 catgtaaatt acacgtaaag ttaaaacaaa tgacatcgaa aagaagagta gccgttaggt
     3061 gtttttagag agtctctggt tggtggaaag aggcagcagt taaccgtgaa acacatcttg
     3121 gagtcgattt tctgaagcgt atagtaggaa aatccgggga gcccgttaca gctgcggtcg
     3181 ttgctagatg accagataag gctggctttt ccgtgaggag tcggtaaaaa aaggtggaac
     3241 cacgtgatta acgtcctttg atcttcggat caaaggactt tttgttttca tctgaacggc
     3301 aacgactgtg tgaggttttt tctgcaagga aaaaataaac agaggtggaa ccacgtataa
     3361 tacgtcctct tggcatttgc caagaggatt tttttatttt aaaggaggaa aagaagatgg
     3421 agtatttgat tgaagttatg ccgcaattat tagtaggagc actcacaaca ttgaaattat
     3481 ttttatttac cttgattggc tcattaccat tagggattgt tttagcatta ggattgtctt
     3541 tgcattttaa gccgctacgc tacttgcttc aaacatatgt atgggttatg cggggaacac
     3601 cgctgttgtt gcaactgatt tttatttttt atgggttgcc gacgattggg atcgtttttg
     3661 atcgtttcga agcagcactc attgccttta tcttaaacta tgcagcttat tttgcagaga
     3721 tctttcgtgg agggatacaa tcgattcctc aaggtcaata tgaagcagcg caagttttgc
     3781 aattaagtcc ctttcaaacg gtgacgaaaa tcgtcatccc acaagtgaca aagattgttt
     3841 taccttctgt agggaatgaa gttatcaact tggtcaaaga ttcttccttg atctatatat
     3901 taggtttagg tgacgtaatg cgtgctggga aaatcgcgat ggaacgcgac gcgactttac
     3961 tgcctttgct aggcgtggca atcatctatc ttacattgac tggtattcta acaatcataa
     4021 tgaaacaagt tgaaggaaaa ttaaattatt accgctaact gataggagga agaaatatga
     4081 ttgaattgaa gaaagtgaac aaagcatttg gtgaaaaaaa aataatcaaa gatttgaatc
     4141 taatcattcc agatgggaaa acattagcta tcgtaggtcc ttccggtggc ggtaaaacca
     4201 cgcttttacg tatcttggct ggcttagaaa gttctgacag cggagaattt tatttagacg
     4261 gtgtaccgtt caacccattc gagcttaaaa tgaaagaaca agttgtagga gtcgtattcc
     4321 aagacttcca gctatttcca catctgaata tttttgaaaa cattacctta gcaccaaaaa
     4381 tggtattgaa acagacaaaa gaagaataca ccaaaaaagc agaagaatta gcggcttcat
     4441 tagggattag tgatttattg aatatctatc cttatcagct ttctggcgga caaaaacagc
     4501 gggtagcgat tgccagagca ttggctatga atccacgggt attggcttat gatgaaccaa
     4561 caagtgcttt agatcctgaa ttacgccagc aagtcgagcg tttgatccta tcattgaaag
     4621 aagatggggt aacccaaatt attgtcaccc atgatttagt atttgcagaa aatgtcggtg
     4681 atcaattact aaaagtttcc ccaattcatt aagaaaggag aagatagaga tgaaaaaaac
     4741 cagttttcta ttcgctttga tcgcaggtct tcttcttttt gcaggctgtt cgaacaaaaa
     4801 aacttcagca gataattggg cgactatcaa cgaagagaaa aaaatcgtta taggtttaga
     4861 cgatacattt gttcctatgg gatttagaga taaagacggg aagctcacag gatttgatat
     4921 tgatttagcc aaagcagtct ttgaacaata tgggattacg gtagactttc agcccattga
     4981 ctggtcaatg aaggaatttg aactgaacaa tggaacgatc gatttgatat ggaacggcta
     5041 ttccaaaacg ccggcaagag aaaagaaagt gcaatttaca aaaccttata tggaaaacga
     5101 tcaagtgctg atcacaccta aagaatcagg aatcacaaac tttggacaaa tgaataataa
     5161 gagattaggt gcgcaaaatg ggtcatctgg ttatgatgta tttacgaaac aaccagaagt
     5221 attaaaagag atagtcaaaa accaagatgc tgttttgtat gatagtttta atgaagcatt
     5281 gatcgatctg aaaagcagac gggtcgatgg tctgctgatg gataaagtat acgcagacta
     5341 ttatttgaaa caaagaaacg aactagaaga cttcaacata acaaaaggtt cttatgagag
     5401 tgaggatttt gcagttggtg cacgcaagtc agatagtaca ttagtgaaaa aaatcaatga
     5461 agccctagat caactagaga aaacaggtga attccaaaaa atatccgata aatggtttgg
     5521 cgaagatgtt acgccgagaa attaacaaat aggggactgt gacaaaagac gtgtcacagt
     5581 ccctttttcc gaataaacag tgttctcctg ccaactcctt gtgactcgga gctgaatggc
     5641 aggctcacaa cctcttggta aacggtgttc aaaagccagt tt
//
LOCUS       DACEPD010000125         5586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.132, whole genome shotgun sequence.
ACCESSION   DACEPD010000125 DACEPD010000000
VERSION     DACEPD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.132"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            142..2202
                     /locus_tag="IW797_002554"
     CDS             142..2202
                     /locus_tag="IW797_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225304.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2285..2854
                     /locus_tag="IW797_002555"
     CDS             2285..2854
                     /locus_tag="IW797_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1225305.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3029..3835)
                     /locus_tag="IW797_002556"
     CDS             complement(3029..3835)
                     /locus_tag="IW797_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225306.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3973..5487
                     /locus_tag="IW797_002557"
     CDS             3973..5487
                     /locus_tag="IW797_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ1225307.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         1988 a          967 c         1129 g         1502 t
ORIGIN      
        1 ccgattttct tgaaacaata aaaaatcaaa aaaaatactc tcctaatagt ccatcttctt
       61 ttgattttat ttatataatg aaatcatctt atgaaaatta ctacttcatg tttagacaag
      121 agtagggagg actaagaata aatgtgttca aaaagacgtt caaaacaact aaataaacac
      181 gcttgtcgtt tagataagga acggtcgtgt tctgaaaaac actacgttaa gtttatgatg
      241 cgcacgatgc tgatcagttt atctgtaggt gtctatatgc atgtacccgt tcaggcaacg
      301 acacaaacca tcaaggtcgt acgtacctct gtttctgaga ccactgattc ccctatcact
      361 attactcata ctttcactga tgcaaatgga aaacagctac ttcctgcatt gacttcctat
      421 aataaagtca gttatcctcc aacgataaac cactactggt taattcctaa caaaacatta
      481 tggacatcgg aaaccggaag cagtgtgccg ctttctatct tttccgatat gactgatcct
      541 acaaaaggtg atcagttaaa acagatcact gatattttga atcatgcttt gacagatata
      601 aaggacggtg gaagcgcaca agtttcttat gtatatgaaa aagattattc acagtttgaa
      661 agaaagaaaa tcgatgttct ggcagggaca aaagtttcga tatctgatct ggtagaaagg
      721 ctgaagaacg cagatggaac acaaggagat atgtccaaag taatttcaaa caatgggaat
      781 atcgtggata cgactaatac aggaacatct actatttcct tgtcctattt tgatccaatt
      841 gctttaaaaa caatgactgc atctgctgtg atccatgttc gtgctgatca aacagctatc
      901 aaaggaaaag atcttcactt aagaacagga gagagttacg cccctaaaga tcttgttgaa
      961 tatgtaacaa aaagcgatgg aacttcagga aataaagaag aaatcaaaat gaacgggaca
     1021 gtagatacta caactccggg agaatatccc gttgtattaa cttatataga ctcgaaaaca
     1081 tcaaatgtgg cagaaacaac agctgtagtt tatgtagaag catctgctgc ttcatcgact
     1141 gtcatcactc attttataga tagtcaaaca aataaagaac ttcaagcaga aacacatatg
     1201 agtagtgtaa atcgtattcc tgatttgcgc ctacctgctg aattttctga tcgagtgcca
     1261 aatatagatt tatcgactta tgaacaaaat gggaaagtca cttctatttc tgattggatg
     1321 caagcaaacg gattagacag aaaaagtcac tggtcggaga tccttgaagt acttcactca
     1381 gaactgatga ataataggaa gcaatcagga ggggaaatta cctttactta tgtttatata
     1441 ccagacaatc gttcattaga aggtcatgat cttattgttc atccaaacga aaaaataaac
     1501 aaagaagagt tgatccaatc agcaacaaat tccttaggta aacccgtacc gttaaaggat
     1561 gtatcggtta caatcaatga agtaccattg gaggatggtt atttgaccaa acaaacaggt
     1621 gattttacga ttatgtatag ttatcatgac ccgtattcac tccaagaact ccatgctcag
     1681 tcactattac atgttttacc attaaacaaa cagatagatc aaaaagaaca aaaaagacaa
     1741 gaagaaagct tgtcagaaaa aaaacaacaa atggctcatg cagtttctcc aacagggaaa
     1801 gtaaaacaaa caagaagcct agagttagca agaatatcta gaaaaatctc agaatctaat
     1861 aaaacgataa ggcaagaagc tcatttggcc ccacaagttt ctggattgga aaatgaaatt
     1921 ttagagccta aaataaaaca acaatcagaa aaagaatttg ttgctaaaca gtcggaagat
     1981 cagaaggaat cagatgacca gaaggaaacg aagaaaaaac gcttgtcaac tattgattcc
     2041 accgatgagc atctgcagcc gcaagtacca cttcctagtc cgggaacacc cgcacctggt
     2101 ggaggtattc cttcttctgc tttaggagat ttcatgcgag gtatggcagg aacatgggta
     2161 tacgctaatc gaaatgaatt tgacgcagat gactggttct gatttatatc tctatctgtt
     2221 atagtcaaat aagtagctga gtcatatgtt atttggcata atggagacga ggagggatag
     2281 aaaaatgtta gataaaacgt taccttacgc agaaatctgg atgacaagac cacttcaaca
     2341 aaatgttcca atcacgcctt tagcggaagg ttttgaattt cgattttatc aaaaagaaga
     2401 tgaatctgct tgggcggaga ttgagacttc cgttggggaa tttcaaacga cagatgaagc
     2461 acttgagtat tttcaacaaa actttgctcc ttatcctgaa gaacttgaaa gaaggatgtt
     2521 ttttgtagag acaaaaaaag gagaaaaagt agcaacctgt accgcttggc gaaaaaaatc
     2581 aagagagcaa gtgtatccgg tttttcattg gttagcagtg aaaccagatt accaaggaaa
     2641 agggctcgca aaagcattga ctgccagagt cttgcaagaa tttcctaccc tccatacgga
     2701 cggaccaatc tatcttcaca cacagacatg gagccatcag gcgattgctc tttataaaaa
     2761 aatgggtttt acttttattg ctgaaaatct agacggcaca cctaatccag attatgaaaa
     2821 agttatgcag gttttaagta aattagataa ataacgaaat ggtgaaataa gtcctcagta
     2881 ttgggataaa aaacgaaata gtcattcctc atatcttact tttttgagtt aatactatcg
     2941 gataactaat ggcatgccat acattcaaaa aagaaggggc agtgacaaaa gtattgtcac
     3001 tgccccttct cttgtttttt agcatgtttt atcgtttgat ttttttagga ataaaattct
     3061 tgatgaacgt aaatttttgg taatcgtttc gactatagag gtaaccaaat acactgaaaa
     3121 caactgctcc aataaaatta acaaataaat ccttcatagt atccatgatt cccacgtcta
     3181 agtaaccatt ttggatgatt gttttatctg ttttaccatc tgcattttga ctttcaatga
     3241 ttgtccgagt gatatctgga acatgataga cagtgtttcc gtctttactt aactccacac
     3301 tattgatttg ctggatgatc gtatcttttt gcatatcgaa ttgcagtaat ttatctgctg
     3361 aaaattccac aaactcccac ataacaccaa ctgtcatcga aaaacagaag gaaacaactg
     3421 ctagtaataa aggagtcaag ttcatggttt ttgtattttt attgagcaaa taaacaaggg
     3481 aaaagccgac acccccagct aaaaatccgc tgaatgcatg tagaaaagta tcccacattg
     3541 aaaaatagcc ataaaaatca cttaactctc ctaaaactgt tgaactaaaa ataaaagata
     3601 acaaaatgat ttctagcata tttggaatct gtatctttaa tatcttttcc agaaaataag
     3661 ggatttcaaa taaaattaat gtcagtaata aaagcattgt atttccccag tgttggttcc
     3721 agacctgatc cactagcata ataacgacga tgatacgtaa taaaatatag attccgtttg
     3781 tgattctacc tgcagttttt gtactgttcg taaaaaaggt tttcaaatga ttcataaaat
     3841 aactccttca tgtatatcct taattatact aagaagcgtg tgtttaccaa agctatatga
     3901 cttaaagaaa aacagagttg atattgtgaa aaaaatcact taatatagta taaggagaaa
     3961 agggggatac aaatgaaaaa attagttgct ggtttattaa cagccgttgc acttcttgct
     4021 atgagcgcat gcggcgcaga tcagaaaaat acagcgcaga caagttctag tacgaaggaa
     4081 tctgccgaag tatcatcagg cgcttctgaa caagtatata cagatccaaa agaaatgaaa
     4141 gaatcttatg atgtcgtgat tattggagct ggtggtgcag ggatggcagc agctatcgaa
     4201 gcaaaagatg ctgggttgaa tccagtaatt ttggaaaaga tgcctgttgc cggaggaaac
     4261 acatctaaat catcaagcgg tatgaatgct tcagaaacaa aatttcaaaa agaagaagga
     4321 atcaaagact cgaatgatgc cttctatgaa gaaacgctaa aaggtggtgg aggaacgaat
     4381 gataaagaaa tgcttcgata ctttgtcgat cactctgctg atgcggtcga ttggctagat
     4441 caaaacggta tcaaactgga caacttaacg atcactggag ggatgagtga aaagaggaca
     4501 catcgtccgt ctgatggctc ggctattggt ggatacttag tggaggggtt attgaataac
     4561 gtccatgagc gggaaatccc tatttttgtc aacgcagatg tgacagacat taagaaaaat
     4621 ggggaaacgg tcaatgaagt gactgtacat gttcaaggag aagaaccaaa agaggtcacc
     4681 ggaaaagcaa tcgtagttac cacaggcggt tttggtgcca gcaaagaatt tattgaaaaa
     4741 tatcgtcccg atttagctga ttatattacg acaaatcaag aaggatcaac tggagatggt
     4801 atcaccatga ttgaaaaatt aggtggacaa accgttgata tggataaaat acaaattcat
     4861 ccaactgtgc agcaggatga gggggtcttg attggtgagg ttgttcgcgg agaaggagca
     4921 atcttggtcg atcaagaggg gaaaagattt gtcaatgaaa tggatacaag ggataaagta
     4981 tctgcagcaa ttacagcatt gcctgaaaaa tcagcttatt tgatttttga ccaaggcgtg
     5041 cgtgatcgtg ccacagctat tgaattttat gaccaaaaag gatatgtcac aactggaaag
     5101 acaatggaag aattagcaga aaaaatcgat ttaccaaaag aaacactagc aaaaacagtt
     5161 gatggatgga atacggatgt tgcggagaaa aaagatgctc aattcaaccg tgccacagcc
     5221 atggaacatg acttatcgtc tccgaattat tatgcgatca aaatcgcacc aggaatccat
     5281 tacacgatgg gcggtgtgaa aatcaatacg aacactgaag tattagataa agaaaaccag
     5341 ccaattaggg ggttatatgc tgctggtgaa gtcacaggtg gcttacatgg aaacaaccgg
     5401 atcggtggaa attcagttgc ggagatcatt atttttgggc gacaagcggg catacaagca
     5461 gctcgatttg cttcggaaaa tgaatgaaat agaacgtaaa taaaaagagg ttgtgaaatc
     5521 agcgttttga gctaataccg agggtcagct gattgaacac tgtttagtga agaggttgtg
     5581 aaaaag
//
LOCUS       DACEPD010000126         5110 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.133, whole genome shotgun sequence.
ACCESSION   DACEPD010000126 DACEPD010000000
VERSION     DACEPD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.133"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(159..416)
                     /locus_tag="IW797_002558"
     CDS             complement(159..416)
                     /locus_tag="IW797_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225308.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            579..1553
                     /locus_tag="IW797_002559"
     CDS             579..1553
                     /locus_tag="IW797_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ1225309.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            2283..2600
                     /locus_tag="IW797_002560"
     CDS             2283..2600
                     /locus_tag="IW797_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225310.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(2804..3169)
                     /locus_tag="IW797_002561"
     CDS             complement(2804..3169)
                     /locus_tag="IW797_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225311.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            3377..3859
                     /locus_tag="IW797_002562"
     CDS             3377..3859
                     /locus_tag="IW797_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225312.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            4056..4946
                     /gene="pstS"
                     /locus_tag="IW797_002563"
     CDS             4056..4946
                     /gene="pstS"
                     /locus_tag="IW797_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ1225313.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1770 a          730 c          893 g         1717 t
ORIGIN      
        1 cctaaaaaaa tgtaaagtct tatctctttt cttaatgcta taaattcata ataacattat
       61 ggacaataaa tttaaaattt attcagactg tagacaaact cctaaattta gagtgttgtt
      121 tgcagtctga cgactcaaaa atgagtcgta taaatcaatt atagtaataa gtcattgatt
      181 tctttaaatt ctttgtcaag ttctttttca tcatattttt catatttatc agcttcatga
      241 agaacttttt taatttcatg aatagccttt cgttcaacta accatttttt taggttatgt
      301 tttttttctg gattttcaga taacgtatca agttcgtcta actctttatc cagtttattg
      361 acaacagaaa caattttgtt tacaactttt tcttctttgt tttctaattt tgacatttat
      421 cttcacgtcc tttttatttg ataatttaag tatagaactt tgatatagtt tatacaaata
      481 aaaacgcttc tttgtgtatt ctgtgctttt cttttggaat gaggaaaact ttggagtaat
      541 atgaacgtgg acagaaaaat atttaggagg aataaactat gtgtacgtct attacttatg
      601 taacaagtga tcattatttt ggaaggaatt ttgattatga aatatcttac aatgaagtag
      661 tcactgttac tccaagaaat tataagttga attttcgaaa ggtaaatgat ttggatactc
      721 attatgcaat gattggtatt gccgctggta tagctgacta ccctctttat tacgatgcga
      781 caaatgaaaa aggattgagt atggctgggc taaatttttc tgggtatgct gattataaag
      841 aaatacaaga agggaaagac aatgtatctc cttttgaatt tattccttgg attttaggac
      901 aatgctcaac agtaggagaa gctaaaaaat tgttaaaaaa tatcaattta gcaaatataa
      961 attatagtga tgaacttcct ttatcccctt tacattggct attagctgat aaagaaaaat
     1021 caattgtcat tgaaagtatg aaagatggac ttcatatata tgataaccct gtgggcgttc
     1081 ttaccaataa tccttcattt gactatcaat tatttaattt aaacaattat cgtgtcttat
     1141 cgagtgaaac tcctaaaaat aatttttcaa atcaaataag tttgaatgcc tatagccgcg
     1201 gtatgggagg gataggcttg cctggagatt tatcctcagt atctcgtttt gttaaagcga
     1261 cttttacgaa gctgaattct gtatctggag attcagagtc agaaagtatt agtcaatttt
     1321 tccatatctt aggttcagta gaacaacaaa aaggtttgtg tgatgttggt gatggaaaat
     1381 atgaatatac aatttattct tcttgttgca atgttgacaa aggaatctat tattatcgaa
     1441 catatgaaga cagtcaaatt actgcaattg atatgaataa agaagactta gatagtcata
     1501 agttaattag ttatccaatt atagaaaaac aacaaattaa atatataaat tagttaatgt
     1561 gttgtgattg attatttaat atagatataa tacaaaaagg caaagatatt ttcaaaacat
     1621 tttttcttga tgtgatatcc ttgtcttttt tgtaatgttt ttaacttatt gcttttgcaa
     1681 aatttattta cctgttttct ggatcaacga tagtgtatat tatgctatcc ttgtttggat
     1741 tttaggacgt ttaaagaggc acttttttac ataacttaat ccatagacct tttaagcatg
     1801 ctgtgagata ataataaaga agagtttaaa gcgcacccca aaccacttcc ccataagtgt
     1861 attacgcttt aaactctttt atatttgaat tcactaagaa gcataccata tttttgaaaa
     1921 aaagtgagaa aaaaggctta taattaggat ttagagtaat taattagatg tttattttat
     1981 aggactgata ttatagatta caaatagtat cgtatttcgc aatcttaaaa ttatcttctt
     2041 actatgaata agataataaa atttgtattt gtccttttta ggtaacttat tttaatctat
     2101 atacctttct ggatcaacga aagtatactt tatatagtca taacgccgat ggtaactatg
     2161 cactgtaacg ataatgctgc taaaatatct ttttacgaaa aacatcacaa ggaaaaactt
     2221 aactatggta ttatctaaac ataatttttt cggtatttaa tatttaatta ggagggaata
     2281 ttttgctttt ttatagtaat tttatcttaa tagtagcgat attattactt ctgaatatat
     2341 ggatttttga taggagtaga aatgcaagta taggttttag aactaaacga agtctgtctt
     2401 ctaaaaaaaa ttgggtatac tcacaaacta ttttttatgg aggaattgtt ttaattagtt
     2461 tactttcttc aacactttat tctttgaata ttatagatgt ttccacctca aattcaatat
     2521 ctattattgg aatcattatt gctgcaatta ttacacaact gtttttagtg tttggagaga
     2581 agaaaagaag taaaaaatag aaaaactagt gatagttttg gtgatagtga tattaaaaat
     2641 cagaaaagat agaaaaagca ggagatagat aaaatgctga tatttcagca ttttttaaga
     2701 gtataaaaga gaaaaaaagt aataaatgta tacatgatat aagaaaaaca tatatgtata
     2761 aaaagaagaa agtagcttct ctttgtacat atatggtttg tttttatgga tgccgagctt
     2821 ttttataaga taaggctacc gcccataacg acccaaggat ttgtggaact aagtaaatac
     2881 ctgcaagaag caagatcagc tgggtcggtt ttaaaggatg atagaagtaa cgccattcag
     2941 taaaattggc gcaaaataaa taggaagtga cagtagatat aatattggca atgaataaag
     3001 atataatacg cctgttcctt tgaaaataat atccagcaaa aagagataaa aatatcaatg
     3061 tgatgaaccc gcttacggaa tgataacgaa aatcaagcag taaggcgaaa tgcacataag
     3121 gaacaaaaga taaaagcaac ggaaggtttt tccagcaagt ttttatcatc tttcatcacc
     3181 acctaacaag tgttattatt ttgttagttt tattgtaaat tgaatcctgc aataatcaat
     3241 tactttttca ttatttctag attaaatgaa taaactctca tgccattatc tctaaaaaaa
     3301 aagtttttcc ccctaatttt tttagataga aaattgtata gtatgatgag agaaaaagta
     3361 gtttggggga acgttaatga gaagaaaagc agtaatcgat cggctttatt tatttttttg
     3421 gttttttcct atcgttttgc taccttttac atgggtaaat gctgttgtac aaggtgcgga
     3481 gatattaggc ctgatccaag gaagcggatt tgtggtttct aggaattttc cagctttgac
     3541 tgttttctat ttagtgataa gtgtaggtct cttattacca aaatctctga tcaatcacta
     3601 caattggcag ctgctttact tttcttgctt attagtattt acatctatgt tttctttgct
     3661 gcctcgcttg attatcggcc ctaatggttt gagcgagagt ggattgaagg attcagctta
     3721 ttcctccatg tatttcttga cgatcaaacc atggttctat ttagtaattg cctcatttat
     3781 tcttgcaaca tttttttact tctggacaga aatcaggata aaaagtgaac gatctgtatc
     3841 tataaaaaat aactattgat ggtggatcat cagtaaaaaa gcatatgagc tgcgtaaaat
     3901 gcatgtttct catatgcttt ttttattatt atgtaaagaa tgcgaaaaac gtttacatga
     3961 tttttacata acttaactat atttttacag caaccatacg agggatttac agtcgactga
     4021 taaactttag ttgtaaataa gaaaggagca ataaaatgaa aaaagtattg tttactctag
     4081 gtatgatggg aattctttta gccggatgcg gctcaggatc aggaaataca gatggctctg
     4141 ccacacaaaa taattcaagt gactcaaatg aacaagtaaa aatcgttgcg gtaggttcga
     4201 cagcattaca gccgttagtc gatgcagcac aagaaagctt tgtacaagaa aatccaaatt
     4261 atcaaatctc tgtacaagga ggaggaagcg gaactggttt gagccaagtc gaagctggag
     4321 cagttactat cggaaactct gatgtatttg cagaagaaag agacggagtt gatgcttcaa
     4381 aattagtgga tcataaagta gcagtggtcg ggatggcgcc aatcgtcaat aaagatacag
     4441 atgtcaaaga tatcaccaaa caggaattga ttgatatctt tacaggaaaa atcactaact
     4501 ggaaggaagt tggcgggaaa gatcaaaaaa tcaacgtcgt gaaccgtgca aacggcagcg
     4561 gtacccgcgc gacattcgaa aaatggggac tggatggtgc tacccctgtg cagtcccaag
     4621 aacaagattc atcaggaaca gttcggcagc tggtaagtca aacaccagga gcaatcagct
     4681 atttagcttt ctcatatctc gatgattcca cacaagcact aagtatcgac ggtgtagaac
     4741 caaaagaaga gaatgtagca gataacagct ggggaatctg gtcatatgaa catatgtaca
     4801 cgaacggaaa accttcccct gaagtccaaa aattcttaga ctacatgatg acagaagaaa
     4861 tccaagaagg acctgtcaaa gaattgggct atttaccaat cacaatgatg gaagtagaac
     4921 gtgatcacga aggaaatatc aaataactag cagataaaca tgccacggta gtcgttacta
     4981 ttaaggaaaa atcaactatg tttcccacat atacattttt cctttaattg tgaaaaatag
     5041 ttcctcatat tttgccgttt tttgagctaa taccgagggt cagctgatta aacaccgttt
     5101 attcgaaaaa
//
LOCUS       DACEPD010000127         5085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.134, whole genome shotgun sequence.
ACCESSION   DACEPD010000127 DACEPD010000000
VERSION     DACEPD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.134"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            51..1358
                     /locus_tag="IW797_002564"
     CDS             51..1358
                     /locus_tag="IW797_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ1225314.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFDNLF"
     gene            1477..2676
                     /locus_tag="IW797_002565"
     CDS             1477..2676
                     /locus_tag="IW797_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ1225315.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYAWQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(2703..3971)
                     /locus_tag="IW797_002566"
     CDS             complement(2703..3971)
                     /locus_tag="IW797_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ1225316.1"
                     /translation="MEKQLPSPMRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLITLTL
                     FGTITTHLFGGSVGREGTAVQMGGSLADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(4134..4781)
                     /locus_tag="IW797_002567"
     CDS             complement(4134..4781)
                     /locus_tag="IW797_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAQ1225317.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILNHELPEIDTFLTTVLDVKLTKAQAERALEKLKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
BASE COUNT         1558 a         1046 c          946 g         1535 t
ORIGIN      
        1 gataaaattt taatttgtag cgaaacaaat taattacaag gagatcggat atgtccataa
       61 aaaaaacacg tctagtccta ttattggcta gtctcattct ttgccttgct tcttttctac
      121 cagctgttgc tgtgcaagct gaagatacgt tcaaagtaaa tgcaaaagca gcttttgctg
      181 tagatgcgca aagcggtaag attctttatg atcaagatgg agaaaaaacc atggggatcg
      241 cttcaatcac aaaaatcatc ggtctttaca tcgtccttga tcaagtaaaa gaaggaaaac
      301 tgtcatggga tgataaagtt tcgatttcag attatgcaga aaatttgagt atcaccccag
      361 atttatcgaa tgttcctttg cataaagaaa acacctacac tgtcaaagaa ttattcgact
      421 ctgcaatcat ccagtcagcc aatgcttcaa tggtggcctt ggcagaaaaa atctctggca
      481 gcgaagctaa attcactgaa cgaatgaaag aacaattgaa agattgggga atcaaagatg
      541 caacaatcgt taatgcatca ggattgaaca actcttatct tggcgaaaac cgaccagaag
      601 gcactggaga aaacgacgaa aatcaaatgt ctgctcaaga tgtggccatc gttgcccgcc
      661 atttgatctt ggatttccca gaaatactgg atgtatcaag tactacgacc caaatgtttg
      721 gcgaaaatac gcaatcacca gtggaaatgg tcaactggaa ctggatgctc ccaggattcg
      781 ttaattacaa ggaaggcgtg gacggattaa aaactggtac gactgagctt gcaggtgctt
      841 gtttcgttgg tacgatcact aaaaacgatc aacggatcat cactgtcgta ttgaatgcgg
      901 acggccatgc ggaaaacccg agtgcccgct tcaatgaaac agaccgtttg atggactact
      961 gctatgacaa ttggtcagaa aaagagctcg gaaaagctaa cgcaagtatt cctagtctaa
     1021 aaaccattga tgtaaaagac ggaaaagaat caacagttcc tgttgtgctt aaaagccctg
     1081 taaaagtatg ggtacgaaac gatatggata ctggcaaatt aacgatcacg ccaacacttg
     1141 acaagaagca agtcactgac aatgagatcc aagcacctat ggataaaggt acaaaaatcg
     1201 gtaccgctac gatcaccttg gcagacgata aattaggcta tttagaagac aacaatgcac
     1261 catcaacaga gatcattacg gataaaacag tggaaaaagc gaatatattc gtgattggct
     1321 ggcgacgcgt cgcagaattt ttcgacaact tgttttaatt gaacaataag aaaaacatct
     1381 ttccatcgga aagatgtttt tttgcttttt ctagtttaaa taacagcttt tttcatctat
     1441 tttcgttata ctgtataaga aaaaggagtt gaatttatga atctaaccag agatctgatc
     1501 tttttagttc tctatcttat tggggtattc tctatcgttt attggcgaaa acagaagaag
     1561 gatccttcct tgttctttta cggcagtacg tggacattta cggtaatctt aacttatttt
     1621 gtcgtgttgg aaatttttta ttcgaccttt tattttttga ttccattgtt gttctttagt
     1681 atctttgctt tttcttattt cacagaaaaa cgaaggctgc tgaatggtct tttattcaat
     1741 gttttcctca tcagtttcgg catctacttg tttgtcttgc tgtatgaaac gcaaaatatt
     1801 ttccttggcg gactgatcgc cttgatcacg attccattgc tacttgtcct tttatttggt
     1861 atttatggat tgattgtttt tcttttttgg aacggggtca cagttttacg tagagaatcg
     1921 cattcattag ctaatctttt aacgttgata ctagccattt ttcttacact gtttcttgtt
     1981 tttgactttt tcttgttaaa atatttgccg caatggataa atgctttgtt tttctgcgtt
     2041 cccctgatct tgatctatct atttatcgta ttctacaact ttttgactgt ctcattcttg
     2101 tatcagttca accggcctcg atacaatcaa gacttcatcg ttgtgttagg cgctgggttg
     2161 atcaatggag aaacagtttc tcctcttcta gccaagcgaa tcaataaggc aattgctttt
     2221 tatcgggctc aaagccgtgc tacattaaat ccgcccatct tattgatgtc tggcgggcaa
     2281 ggagcagacg aaaaagtacc agaggccatt gcgatgaaac aatatgccat ggaacaagga
     2341 atccctgaaa gagacattct tgtcgaaacc aattcgacaa ccactttgga gaacatgctc
     2401 tattcaaagg aaatcatgga tcagcaaatg aaagggccat accgcgcgat ctttagttcc
     2461 aacaattacc acatttttag agccggtctt tatgcgtggc aagccaaatt gaaagcaaat
     2521 ggtattggtg caaaaacagc tttctactac ttgcctaatg cgtttttaag agaatacatc
     2581 gccatcttga tgatgaacaa aaaaagacat atgatcattt gcggattgct tttcgcaggc
     2641 tccgcattct tatcgctgat tactttactt ttctaaaaaa acaaggtgtg acaaaacata
     2701 tgtcacacct tgtttttttc tttcattctc ttaactattg gcaaaaagag tatccctttt
     2761 ttgaccgcta cttgctgggc agaataaata ccgctgttgc ctgaacacat aaagctgatc
     2821 aagcagatca taaagaagta tactgccgct tgtgaaccga acaattcgat ccccatcaca
     2881 aagcaggcaa tcggtgtatt tgtcgcacca gaaaatactc cgataaagcc caatcctgca
     2941 agaaaaggaa cagaaacatg aagcaaaggc gctagtgctg caccaagcgt tgcaccaatt
     3001 tcaaacaatg gtgtcacttc tcccccttga tatcccgcgc ctaaagacaa aactgtgaag
     3061 aataacttcc caataaaatc atagacatgt gcctgtccat taaatgattc ttgaagtaac
     3121 ggaagactca atccgagata acgttgtgtt tgcaaaatca atgctgctgc cacaactaca
     3181 gctccaccta tcgtatttcg ccaaacagga tgaggaatcc attgtgcata tcgcgctttt
     3241 aaaaagacga tcgaacggct gaatacccaa ccaatcaaac caaaagcaat cccagcaaca
     3301 aaaactttga agaacagcaa catactccat tcaggaatca cacccatttg gtaatgtaga
     3361 tgagaaacac catagctttc tgtcatgaaa tttgcaaaca gtgcagcaaa gaagctaggg
     3421 aagatcgctt ctgttcggac tttcccgatt gccaacactt ctaatccaaa taatgttcca
     3481 gctaaaggtg tgccaaatac agaactaaaa cccgcactga ttccactgat aatcaagatt
     3541 tcccgttctg ctttatccaa tcggaacaaa cgtgctgtat tatctgctaa cgatcccccc
     3601 atttggactg ctgttccttc acggccaaca gaccccccaa ataaatgggt agttatggtt
     3661 ccaaacagtg taagtgtaat aagccgcaac gggatttttt cttctccacc atttccttga
     3721 tcgatcacta agttgtttcc acgactagaa agaccgccgt gatacgcata gagataagca
     3781 aaaagcgcac cactaactgg tagtagataa aggagccaag gatgtgatag tcggatatct
     3841 gtcacaactg tcaggctatt taagaaaaaa gcagacaagc tccccatgaa tataccgaga
     3901 atcgctgtga tcaccagcca cttcagcatg tacactaaaa tgcgcattgg actcggtaat
     3961 tgtttttcca taaaaaattc ctcctcaata aaaaaacgcc tactaaagaa aggggctgtc
     4021 cccagacttt agtaggcgtc attagctatc tttcgttagc atttaaaggc gaacaccatc
     4081 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
     4141 tctttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
     4201 cattggtcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaacct
     4261 gctttcgttg tagaaagaaa aagcgacaca ttcgatgttt tctgacagat tgcacagatt
     4321 cccttattta tcgttggtga taaagtaccg ctaatccctt ttaatttttc atcttcgtaa
     4381 tagaccatga attttttctg cgtacctgga tcattccagc cgatgtacgt atgttcttgc
     4441 aagtcgattt cattccaagc tggttgtttg agttttttta cttttcggaa taatttctcg
     4501 atttgtggtt tgctaggctg cataaacggc tgaacgattt gcttcagctt ctccaatgct
     4561 ctttctgctt gcgcttttgt cagttttacg tctaaaactg tcgttaaaaa tgtatcgatt
     4621 tctggcagtt cgtgatttaa tatgtctcga atcttttcag ctgctagtgc ctgaactgtt
     4681 tgtaccgttt ttggatcatt gacagatgag tacgcattta ataactgttc tacttctctt
     4741 tttataaaaa aatattggta tggttttatt gtttgttcca tgattttaaa gtcctttctt
     4801 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
     4861 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgc
     4921 ttattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgaacagat
     4981 agtcaactgc aaacagaaaa aaggactgtg acaaaagtcg tgtcaaagtc ccttgtttcg
     5041 aatgaatggt gttcaaaagc ttgcatctgc ggtaataagc cgaaa
//
LOCUS       DACEPD010000128         5071 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.135, whole genome shotgun sequence.
ACCESSION   DACEPD010000128 DACEPD010000000
VERSION     DACEPD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.135"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            91..342
                     /locus_tag="IW797_002568"
     CDS             91..342
                     /locus_tag="IW797_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225318.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            324..596
                     /locus_tag="IW797_002569"
     CDS             324..596
                     /locus_tag="IW797_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225319.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFMD"
     gene            763..1164
                     /gene="tnpA"
                     /locus_tag="IW797_002570"
     CDS             763..1164
                     /gene="tnpA"
                     /locus_tag="IW797_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1225320.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            1176..2321
                     /gene="tnpB"
                     /locus_tag="IW797_002571"
     CDS             1176..2321
                     /gene="tnpB"
                     /locus_tag="IW797_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1225321.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
     gene            2559..2822
                     /locus_tag="IW797_002572"
     CDS             2559..2822
                     /locus_tag="IW797_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ1225322.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            2816..3166
                     /locus_tag="IW797_002573"
     CDS             2816..3166
                     /locus_tag="IW797_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1225323.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(3190..3804)
                     /locus_tag="IW797_002574"
     CDS             complement(3190..3804)
                     /locus_tag="IW797_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1225324.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            4254..>5071
                     /locus_tag="IW797_002575"
     CDS             4254..>5071
                     /locus_tag="IW797_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HAQ1225325.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYG"
BASE COUNT         1820 a          780 c          947 g         1524 t
ORIGIN      
        1 ttcctataca atgagacacg tgttccaaaa aaacattata aggaaagatt gtcggtagct
       61 gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca ttgcgacgta ttatcgaaca
      121 ctggaagaac tgaaaaagca aaatgcaaaa tggtttttcc aagccttact atgcttggaa
      181 gtaggcgtca aaccaagcac aatcaaacct tctgaatacc aagctttaga actaacgtat
      241 gcgaaattta tcgaaacgaa aaaagctaag actgtttcaa gtgaatggtt agattatttt
      301 gaaaatatca ataagtatgg cgcatgttac atcatgaagt aatcagacgg attcaaaaag
      361 actttagttg tatggaagaa atgaagtact ttgttcaaaa tgttgaagaa cagttatatt
      421 tggaaagcga aacgagtcaa acaaaggtca cagtcagcca taatcgttta gatgtgtata
      481 aacgtcgtcc tgaatcatta aaaaaaggag cggagcttct acgactagaa acagaaaaat
      541 acgtcatgaa tgtttaccta gaaaaaagtg agttggtcga aaattttatg gactagccga
      601 gaacacccgt gattttaatc atgggatgaa tcggattcag ttgtgaccta gctttagcta
      661 ggttaaacaa ctgacaaata taaactaaat aatagtgaaa tgtcttgcaa taattgattt
      721 tgtagaaata catatattaa aataattgcg aggtgttttt gtatgtcaga aattcattat
      781 ggacggggtt atgtctattc tattcaatat catcttgttt ggtgtgtaaa atatcgacat
      841 gatgttttgt atggtcaggt agatattgat gtcaaacagt ttttgaaaca aattgctgat
      901 gataataata taaaaattat agaaatagaa tcagataaag accatattca tttactcatt
      961 gaatgcaccc cacaacacta tattcctagt attgttaaag tatttaaagg cgcatccgct
     1021 agactgctgt ttaaaaaaca tcctgaactt aaacaactcc tttggggtgg acatctttgg
     1081 aatccaagct actttgtagc tactgttagc gaaaatacag aagaacaaat tagaaaatat
     1141 atacaaaatc agaaaaagaa atgaggtgaa tccgtatgct acaacatagg gcttataaat
     1201 ttagaatcta tcctaatcaa gaacaagaaa tattaatgac taaaactatt ggctgttcaa
     1261 gatttgtcta taattatttt ttgaatttgt ggaatcatga atatacgact actggaaaag
     1321 gattatctta taattcttgt tctgccatgc ttccacaaat gaaaagaaat gcagagacaa
     1381 tttggttaaa agaagttgac agtattgcga ttcaatcctc attgaaaaat ctttctgatg
     1441 ctttttctcg cttctttaaa aagcaaaata aacgaccaca atttaaaagt aaaaaacatc
     1501 ctatacaaag ttatacaact aaaaatgtga ataatagtat cgaaattaga aacaatttcg
     1561 taaaactacc taagttagga tttgttaaat ttgcgaaaag tcaagagccc aaagggcgaa
     1621 tcttaaatgc tacgattcga aaaaattcga gtggtaaatt ttttgtttcc atcctgtgtg
     1681 aagaagaaat agttgagttt cctaaatcta attccgctat tggaatcgac cttggtataa
     1741 ctgattttgc aatcctttct gatggtcaaa agattgataa caataaattc acgtcaaaaa
     1801 tggcaaaaaa actcaaacgt gaacaacgta agttgtcaaa acgtgcatta ctagctaaaa
     1861 agaaaggcgt caatctcttt gaagctaaaa attatcaaaa acaaaaacga aaagtagcac
     1921 gactacatga aaaagtaatg aatcaacgca atgacttttt aaacaagttg agcacagaga
     1981 ttatcaaaaa ccacgatatt atctgtatag aagacttaaa tacaaaaggt atgttgcgta
     2041 atcataaact agcaaaaagc atttctgatg tgtcttggtc tagttttgtg actaagttac
     2101 aatacaaagc taactggtac ggtcggaaaa tcatcaaagt agataaatgg tttccatcta
     2161 gtcaaatctg ttcaaattgt ggacacaaag acggcaagaa atctctagaa attcgagaat
     2221 ggacttgtcc tgtttgtcat gctcatcatg accgagatat caatgctagt atcaatattt
     2281 tgaccgaagg tctaagatta catgtcatgg gattagctta gatccaatca tcaaaaaccg
     2341 taggaactac ggggatagct tggtaaataa gagaaaccgc tgtgagtaaa gaaatacgct
     2401 tgcaagtatg ctctattccc aagaatctcg tgactttagt catgagaggt tcaaggattc
     2461 atggaatgtt aatctaattt aatagaaaag gataattaag tatatacgtt ttttcttatg
     2521 tatgttatac tatgtatata cagaaagagg tgtctactat ggaaatgtta cctataaaaa
     2581 aatggggaaa tagtaatggc cttagattgc caaagtatat tatggaatat cttggaattc
     2641 atacagaaga caaagtgaag ataatacaag aagaagtaaa cggtcagaag cggctaatta
     2701 tcgaagcggc taatatagaa aatgaattaa caattgaaca gttatttgaa aattacactg
     2761 atgaaagaaa tcatgtgacg attcaaaatt taggtgaagc agtaggtaat gaaaaatggt
     2821 gagaaaacct agacaaggcg atattctatt attgaataca gcacctcgat ctggacacga
     2881 acagacaggt aaaagacctt atattgtttt aagtcatgat attattgcgg attatagtaa
     2941 cgttgttaca gttgctccaa tttctagtac aactagaaac tatccgctat atgtaaatat
     3001 aaactcagaa tatgaaatga agacaacagg aaaagtatta ttagatcaac taacgacaat
     3061 tgattatgaa gccagagagt gtattttttt ggaaaaagcg catgatagtt tagttgagga
     3121 gttattgatg aaagttagaa cagtttttca aaaagtaagc aaataaaata gaaacagcag
     3181 catgatcaac tattcgtgat tgaccatgct gtcttttttt attctatata cggtttgtct
     3241 cgtaacattg tagtcttttg caatcttagc gatcgcaaca ccttttttta gatcctcaac
     3301 gatgttttta taaacaagac gacgttgggg atcttttgca ttggcactgt atagcttagg
     3361 acgtccttta taaacaccgt tcgcttttgc aatcttaatg ccttgtgcct gacgtcgttt
     3421 agattcggta cgttcttgtt cagcgatcat ggccaaaatt tgaatgatca aatctttaat
     3481 gaatttatcc aggagaggat tgccaattgc ttcggccatg atcggtagac ttgtgatgat
     3541 cagctgcaca tttttcttct tcaagtaatt gactgaagcg atgatctcgt cgtaatttct
     3601 tccaagtcga tcaatagctt ccacaataaa aatatcttga tctcggacga aatccagtgc
     3661 ttcttgaaaa acaggtcgat gagtaatcgt tgcgccagat tgtttctcaa tgaaaattct
     3721 ctctgcaccg aattttttca tttcttcaat ctgccgttgt tcattttgat cgatggatga
     3781 gacacgtatg tatgcaactc tcaaaatttt tacctacttt ctgcaactat ttaaaaaaca
     3841 ccctaatcat acacgagaaa accagatttt tcaatgtcgc ggtcgtgtct agttagggta
     3901 taccttatat agacaaaaac taatgatgga ggaaatagta tgagaatttt ttgtaagtgt
     3961 ctactgataa cttattctca aatgttgtaa ggattttatt catttgttaa tattaaagaa
     4021 cggaactcca aaaaagtccg tactctaatt tgcagtctct cactctttta gagtgagagt
     4081 ttttattaga ttttcaaatt aattagtaaa gtgatagaat aaattgatca tacaaatcaa
     4141 aagaagaagc agggtgatag catacctatg cttctttttt tatgtattta gctatttctt
     4201 ctttacaata tcgaacatat gtttgtataa tgtgaaagaa tggagtgtta tttatgatgg
     4261 attattcaaa agagccagta aatgattact ttttaatcga tatgaaaagc ttttacgcaa
     4321 gtgtcgaatg tatagaaaga aatttagatc cattaacaac agaacttgtt gttatgagtc
     4381 gagctgataa tactggttca ggattgatat tagcttcttc tcctgaagca aaaaagcggt
     4441 atggtattac aaatgtgagt agaccacgtg acttaccaca accatttcct aaaacactac
     4501 acgttgttcc accacgtatg aacctatata tcaagcgaaa tatgcaggta aataatattt
     4561 tcagaagata tgtggctgat gaagatctac tgatttactc gatcgatgaa tcaatcttaa
     4621 aagtgacccg atcactgaat ctttttacga ctgaaggaac acgaagtcaa cggagaaaga
     4681 agctggctca aatgatccag gagaatatta aagaagagct aggattgatt gctacagtag
     4741 gtgtcggaga taatcccctt ttggctaaac tagccttaga caacgaagca aagcataatg
     4801 aaggatttgt tgcggaatgg acgtacgaaa acgttcctga aaaagtatgg aatattcctg
     4861 aaatgacgga cttttgggga attggctctc gaatgaaaaa aagactcaat cagatgggga
     4921 ttatgagcat tagagatctt gctaactgga atccttatac gattaagaat agactaggtg
     4981 tgattggctt acagctgtac ttccatgcta acggaatcga tcgaacagat atagcaatcc
     5041 cgccagaacc gacaaaggaa aaatcttatg g
//
LOCUS       DACEPD010000129         5041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.136, whole genome shotgun sequence.
ACCESSION   DACEPD010000129 DACEPD010000000
VERSION     DACEPD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.136"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(227..1189)
                     /locus_tag="IW797_002576"
     CDS             complement(227..1189)
                     /locus_tag="IW797_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ1225326.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1191..2630)
                     /locus_tag="IW797_002577"
     CDS             complement(1191..2630)
                     /locus_tag="IW797_002577"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ1225327.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2815..4761
                     /locus_tag="IW797_002578"
     CDS             2815..4761
                     /locus_tag="IW797_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ1225328.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1639 a          841 c          816 g         1745 t
ORIGIN      
        1 aaattaaact ggcaaagtcc cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa
       61 tatcatttta gagcatgaaa aaaggagtgg atttctccac tccaaaaaag tctaactttt
      121 gggggtcact acaagttgac aactcctttt tgtttacaaa tcaattggat atctacaaat
      181 tgtattttct aacgaatttt atagacatta aaaaattaat ttaacgttaa atacttcctc
      241 cagaaagtat tgagattggt aaaatataat ccttatttgt cttttcgcct ttaatttttt
      301 tcagaagaat atcaacaata agacttgcaa tttcatcaat tggttgtctt attgttgtca
      361 attgtgggaa aaatttctca ataaaagagg taccatcgta tccaataatt tttaaatctt
      421 ctgggatatt aagtttaagt tgatgagcaa ttttcatagt gagaatggct gttaaatcat
      481 cagaaacaaa aataccatct ggtttgtgat ttataatagt tgactttatt tccatttcac
      541 gacggatagt agacagatta ttaggaattt tgaaaatttt tccatttgga ttttgaaaag
      601 aaaaacctag tgcacgtaat tctgtgggag aatccgtgtt atcatttcct gtaatcataa
      661 tggtattttg acaaccattt ttttgtaaag tcttagctgc aagttttccc ccttcaaagt
      721 tatctgaaga aacaatggaa acattaggag ctaaatttct atcaaaagca acaataggtg
      781 ctctcacacg ctcataatca gcaattccaa gattatggct tgatgagatt atgccatcaa
      841 cttggttggc ttcaagcatt tcaatgtatt ctctctcttt agcaggatca ttttgactat
      901 tgcaaataat cgttttatat ccatgtttaa ataactctat ttcgagatac tcaatgagtt
      961 cagagtaaaa gatattacta atattaggaa aaataagacc aataagctga ggtgactttc
     1021 cttgaagtcc acgtgctaaa ttatttggtt tataaccaag agttttcata gcttcatgaa
     1081 ctttttgttt tgttttttct gataaatatc ccttattatt aatgacgcgt gaaacggttg
     1141 ttgggctaac tccagctaat tcagcgacat cagttaattt tgctaccatt ttaatatttt
     1201 aattcaaagt attttccagt aggttcgcct gatttgattt gaataccatt ttggtgattt
     1261 tcagggaata cacgactagt aaacactttt tctcctttat taataaaaat ctcaatgatt
     1321 gagttatcta caaaaatgtt agcagttgtt tctttagtat caattgagca ttcacgaatc
     1381 gtaccaaagt cagtggcata ttgaatgcct gctttgctgc gatctaaaat aatttttcct
     1441 tctttggtat ctacaatcaa gctaagtcca tttcctttac tatcagagaa aagaagtaac
     1501 tccgatttct gatttggagg aaaagttagt tcaagctcat aagtattatt tgtttctgct
     1561 tttgctgtaa attgttcaga actagcacgt aatgatgtaa tagcttctac tggatattgg
     1621 taaagtttac catcttttat tgaaagttct tttacaagac tcattgctcc ctgataatca
     1681 tacttatcag ttggataatc gatatcaggt agtccaatcc aactgacaat cagagcacga
     1741 ccatcaggag tattgaatcc ttgagtcgca tacgcttcaa atccataatc aagattttga
     1801 atttctgaag cgcccacaag ttttactttt tctatgtcaa gcgattgaca gatcttaaaa
     1861 gtattcggat agatattccc ataatttagc tcagatttat ccaacccttg aggacaataa
     1921 atgagaacag gttttttgtc tacaaatact agatttggac actcaatcat gtattctgaa
     1981 ccagtgccgc caaaatcaag gttgccaact tcctcccatg tttccacgtt attgtcatcc
     2041 gctttgtaaa gtttgataaa gcctgatttt tctaaatttt gtgctccaac aatcgcatac
     2101 aattgtcctt cataattaaa catttgtgga tcacgaaaat gatcagttac atcagcaggt
     2161 ttatgaataa gaacattctc acctttcgta atgttgtagt ctttatccat ccatgctccg
     2221 atttgaagtg tatcacgttc ccaattttca tttcgaacgt ttcctgtata gagtaagaat
     2281 agattcccct ctatttcata tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg
     2341 tctggaagaa gttctgctcc tgttacatga aaatgaacta aatcatctga ttcattgtgt
     2401 acccattctt tcaacccatg agcagctcca aatggccaat tttgataaaa aagcgtatat
     2461 tttccgttaa aaaaagaaaa accattagga tcgtttaaca atcctgtttt aggttcgata
     2521 tgatagctag cacgccaagg tgatcgtttt acattatcct taattttatc catctcatct
     2581 ttagtccaat cagaataagc acgatatcgt atttctcgag gtaagttcat attttactcc
     2641 ttcaatttat ctttttacaa ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt
     2701 catttaaaaa aataaactat atgacaaacg tttgacatat ttttaagata tgctaaaata
     2761 aatttgcatt tcagtgaacc gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat
     2821 tacagtaaag tagcttccga cgtaatacaa gcggtcggaa aagataacct tattgcagct
     2881 gcacactgcg ctacacgtct tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa
     2941 gctttagacg aaaatccaga tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg
     3001 attatcggtg caggtgatgt taatttcgtt tatgacgaac ttatcaaaaa aactggactt
     3061 tcggaaattt caacagatga tttaaaacaa attgctagta aagataaaaa attcaacccc
     3121 attatggcat taattaaatt attatcagat atttttgttc caattatacc tgcccttgtt
     3181 gcaggtggtt tattaatggc attacaaaac tttttaacag ctgctggact ttttggacca
     3241 aaatcaattg aagaaatgta tcctgcgatt gaaggtattt cctcaatgat ccaactgatg
     3301 tccgctgcac cattcatgtt cttaccaatt ttagtaggta tttcagctgc taaacgtttt
     3361 ggagcaaacc aatttcttgg tgctgcaatt ggtatgatca tgacaacacc agatcttggt
     3421 ggctcaactg aatattggaa tatttttggt tatcatgttt cacaaacaaa ctatgcttat
     3481 caagttattc cagttttagc agctgtttgg atattatcta tattagaaaa attctttcat
     3541 aaaaaattgc catcatctat tgactttaca ttcacgccat tattatcagt aatgattact
     3601 ggatttttga ctttcacagt tattggccct gtaatgttat tgttgtctaa tggaattaca
     3661 gacattattg tttggctata taacactact ggatttattg gaatgggtat ttttggagga
     3721 acatattcac ttatagtcat gactggttta catcaatcat ttccagctat tgaaactcaa
     3781 ttactttcag cgtggacaaa tggtatcgga catggagact ttatctttgt agttgcatct
     3841 atggcaaacg ttgctcaagg tgctgcaaca tttgccattt ggttcttaac taaaaattct
     3901 aagacaaaaa gtcttgcttc ctctgcgggt gtctcagcac ttcttggaat tacagagcct
     3961 gccttgttcg gagttaactt aaaataccgc tttccattct tttgtgcttt aattggttca
     4021 ggaattgcta cagcagtagc aggattactt aaagtagttg ctgtttcact tggttcagca
     4081 ggtttcttgg gatttctttc aattaatgca acatctattc cattttacgt attgtgtgaa
     4141 ttgattagct ttgtagttgc ttttgcaatt acttacttct acggtaaaac aaaagctgct
     4201 ggtatcttta aagcagaaga aattgctgaa caaacaccag ttaattcttc aaaagtagca
     4261 acaacagatc caatatctac tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca
     4321 attgttagtc ccatttctgg taaagtgatt agtctttctt cagtaaatga tccagtcttt
     4381 tcatcagaag ctatgggaaa agggattgcc attaaaccaa atggaaacac ggtccattct
     4441 ccagtagatg gtactattca actcacgttt gaaactggac atgcttatgg tattaaatca
     4501 aataatggtg ctgaagttct aattcatatt ggtattgata ctgtatcaat gggaggaaaa
     4561 ggatttacac aaaaagttac tactaatcaa aaagttagaa aaggcgatat tcttggagca
     4621 tttgaccgtt caatgattga gaaagaaggt cttgatgata caacaatgat tattgtgacc
     4681 aatacttcgg attattcaga agtaaatata gtagctgaaa atacagtcac tgagggtact
     4741 gatttacttg aaatcaaata aatatatttc cctgagtaaa gatggaattt tctttccaaa
     4801 atcaaataat atagattaat caaatatttg agaataacat tttatttaat cttttcatat
     4861 atcaattgta tgaaaaatta ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa
     4921 tagtagttat ttacttgtca tgttactaaa taaaaaaata atttaaatat gatatatagt
     4981 catttattta gtgatttcat tctaaaataa agggagaaat gtctatgcta tacggtgcaa
     5041 t
//
LOCUS       DACEPD010000130         4850 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.139, whole genome shotgun sequence.
ACCESSION   DACEPD010000130 DACEPD010000000
VERSION     DACEPD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.139"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            622..1185
                     /locus_tag="IW797_002579"
     CDS             622..1185
                     /locus_tag="IW797_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1225329.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1464..1751)
                     /locus_tag="IW797_002580"
     CDS             complement(1464..1751)
                     /locus_tag="IW797_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1225330.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1741..2070)
                     /locus_tag="IW797_002581"
     CDS             complement(1741..2070)
                     /locus_tag="IW797_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1225331.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2375..2704
                     /locus_tag="IW797_002582"
     CDS             2375..2704
                     /locus_tag="IW797_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1225332.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2991..3476)
                     /gene="vanZ"
                     /locus_tag="IW797_002583"
     CDS             complement(2991..3476)
                     /gene="vanZ"
                     /locus_tag="IW797_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1225333.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3629..4540)
                     /locus_tag="IW797_002584"
     CDS             complement(3629..4540)
                     /locus_tag="IW797_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ1225334.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1584 a          831 c          737 g         1698 t
ORIGIN      
        1 gctttagcgg gtattacaat ttgagtgtaa cataaatggc ttttttattt gtctcgctta
       61 attatacaaa cggcgaagtt aaaacttttt tagcagattt taaagtttcg tcaaaactaa
      121 tatattctgg tagctgagca ttcaaatagc tttcagactt cagaagaaaa ttacgagcat
      181 ttttatagtc catctctaat aaagttattc tttttttatc caatacattc agctcaattc
      241 cttataatat tctattcctt agtatctcaa cattatttac tttataaaac gaataaattt
      301 tgtagttcta attcaaatat tgcatgttat atctaatatg ggagcatttt ataattttca
      361 ttgtgataaa ctcgttatta actttaggat acactagtta ggattaaatt aggtatctag
      421 aaatttactt aagatttttt tgtttatttc gtatacacac tcgtatcaaa aacaatcgat
      481 tatgagacaa aatgaaatga cctcttttat taaataaaag aggtcatatt ttgtatcata
      541 acttgttgca ttaactatgt atctataact cccttttatg atacaatcaa gccagaatta
      601 aagtacgaaa ggaagaaaag catgagtgga tacatttatg gttatgcccg agtgagtaca
      661 cgttcacaag aactaaatcg tcaattagac ttactcaatg aacaaaattg taatgaaatc
      721 ctaactgaga aaatgacggg aaccaaagcg gttcgtcccc aactaaatcg tttaaaagat
      781 aaaattcgtc cgggagatac ggtaattgta gaaagttttt ctcgattggg tcgtagtaca
      841 aaggatttga ttgaccttgt tacctatttt gaagaacacc atgttaagct ggtgagcttg
      901 aaagaaaact tcgataccag cacgcctcac ggacgcttaa tgatgactgt cttccaagca
      961 tttagtcagt tcgaacgtga tttaattgtt gaacgtacca aagaaggttt gaaaagtgca
     1021 cgtgcgagag gtcgtaaagg tggccgtcca cgagtgaacc agaaagatgt tgatcgagca
     1081 gtaaaattgt ataaaagtca ggtttatagc gtaaaagaaa ttacggaaat gactggtatt
     1141 agcaaggcaa cactctaccg ctacttaaaa gacaataggg aataatctat tttaatcttt
     1201 aggaatctat aatatttatg ggagaattag agtgattttt ttattattac taaaaaacaa
     1261 tataaaataa cgcattttaa gccctctttg tgcataagga tgaagctttt aatttgaaag
     1321 aggcttaaaa tgcgttttaa ttttttctat ttttcataaa tatataggtt agtggtttaa
     1381 aattataggg tattacaact ctcagctgtt aaaaattatc ttttaaagtg tcgtctatcc
     1441 tctcaagttg aaacattggg ttcttaccta atctttttta attgttcata aaaattttca
     1501 tgcgttccac atagtaagat aggtatcact gcattatctt taatttcata tgcaacgcga
     1561 tatgtcgtac ctgcatactt gaatccaata gcccaaattc ctgataaatc acccgttttt
     1621 tgttctcccg aatgaggacg aactgctatc tcatcataaa ttatcgttaa aaattgttct
     1681 tttaatgttt tctcttttaa tttttttaga tactttatta cgtgtttaga aggtagaatc
     1741 tcataagcca atttctgctg ccaactcctc ttttgtcatc ggtttagaat ttgcaacagt
     1801 atcttcagca atagatttaa atgcagaagt taacgcattt tttctatttc taaattcttt
     1861 taagatctct tgtttgctat aaccttctga aagaatatca gccaaaatat cttcactaaa
     1921 atcaaaaaat tcttttttag gctctacaaa ctcataaagg ataccattct ctacaagctt
     1981 agcttctact tcaacaccat tgggcacatc gaattcttta gggaccgtta acataataga
     2041 atttccttgt tttcttgttt ttgtaatcat attatttcct ccattatttt acatttcgta
     2101 attacttagt aattacattg tacgctattt ttagtagaaa tacaaaataa taaaactcct
     2161 ttagaacaat tttcaaagga gttttatttg attaggaaat tatgtagaat aataatatgt
     2221 ttttaacttg gaatcaaaaa cgcacttgca agtaagttta cttcaataca tgaaattgct
     2281 tataatagtg tattggaatt aaggtagatt gacaaaagag tgatcaaata ggtatacttc
     2341 attcaaataa tcgtttgaat gtgaggtgag atgaatgaaa aaagtttgcc aagtgactgt
     2401 cgtacataaa gaaaaagtaa atggaataaa aaataaattg gatcaaaaag atttttcaaa
     2461 cttacttgtg ttagggaaat gtttcagcga tttttctaga attaaaattt tctatgcttt
     2521 agaaacctat aaggaaatgt gcgtctgtga tttagcagaa atacttactg ctagtgtcgc
     2581 tacaacgtca caccatttac gttttttaaa aaaacatgga atggcaaaat cacgtcaaga
     2641 tggaaaagta gtttattact ctttagcgaa tgaagacatc ctttccgcta ttcgtgtttt
     2701 ttaaagttat cagaaaatct atctatgaaa tcttagtttg ggggtaccgt caggaaaatg
     2761 cggatttaca acgttaagga aatttattaa agagaccaat tcctcagcac taaaattgat
     2821 aattgggctt tctttgttac tatagtgtta ggtaagcttt ttactttatc accggttttc
     2881 ccctcacttc acacctacgg gcgagtttcc ccacatcccg ttttccatct aatccaattc
     2941 attcaatatc tttatgtgta tgaaagatca gattgcttga tttagacatc ttacacgtaa
     3001 tttattctta aatgggtacg gtaaacgagc aataatacga gcaaaagtat acctacaaaa
     3061 ataataactc tgtctaattt tttttgattc atatgcttat tgcttaaacc atataatttc
     3121 agtccaagaa agcctccaac agtatttgta attacatctg ttatgtctgt cgctccaata
     3181 gcgaagataa attgaattat ttcaaaagta agacttaaaa ccagtacaaa agcaaactta
     3241 ggtaaaaatc cgatttcttt aaaattgaca ttcaaaagca agccaaatgg aataaagatt
     3301 ataacattat ctatcatctc tctgaaattc ccagtagcag taaatggagt caagttaaga
     3361 cttctttgat gataattaaa tactgataaa atattgtatt gtaatttgaa taacactaac
     3421 cagattagtg tcactaaata taaagctagc aatcctctag ataatatttt tcccaatata
     3481 atttcctcta attcttttgt ttaagttgta tcattagtat agcaatgtcc tagttaacgt
     3541 gggttttaaa ttaaaaagct aacgttaccc caaaagtgct agtacaataa tgctgttcat
     3601 taatttcgtt ggtttcattc cgccatcctt acctccttga attagtatgt gttgatccgg
     3661 gaaacactgt cacaattaca ccgtctatat tgtttcctga tatctcataa cgaagattag
     3721 tcggcacatg aatggtggta tttttagtaa caggataata aaagatctca tatttttccc
     3781 catttacact aacagaaatg gttttttctt cttttaggta atccatatat tcctcgagaa
     3841 cgaaattctt ttctttcata atcgcactat gtggtaaacc aacatagcga atatgccatg
     3901 gttcatattg aattcctgtt aactctgttt tgtcctctgg ataacgtaaa atgaacccgt
     3961 atttccaagc attttcttct atccactttc cttcaggggc tcgttccatt ttcgtcaagc
     4021 ttgatcctac atctagtgat aaacctgaat tatgctcact ataacctgct ggtaaggcat
     4081 actcagcccc catttcttgg taaagcacac tttgctcatc aaagtctcga tagccactat
     4141 taataataaa atgactaacg ccacccttta cagcatcatt gaccatctct gaaaattttt
     4201 gtgctatttc ttttgacata taaatattac tatcaagcaa cccgtatcca tttattaatt
     4261 cgtcatgttt agataaattc acgatatctg acttcacact ttcttggcga acaggatatt
     4321 tactattgat taatagcaga tttccttgat aaacctgttc ttctgtaatt gttttctctt
     4381 gggtattttc agaagtccca ctattttcta aatgttcttt gggattttga tcataatttt
     4441 gaaattcgac tttttcctga gaaaacagtg cttcattaac gtagtcataa cctaagtata
     4501 ttaagaataa caataacaat aaaaaaaaca acttcttcat tttcagtctc ctccttaact
     4561 atatagttgg aagtatgaaa tatttctaga atcttcccct taaacggcac aatctcgttt
     4621 gagattgtgc cgttttgccc cgtaaattac agtcaattat ttcgccctac tggatttact
     4681 attatcacca atgtagatga attttcataa gtgataccca tgctgaattt aacgaggctg
     4741 aactgcctgt tgacaatggg cggtgtgaaa taccacccct ctatgcgatc agtcttatta
     4801 tcaggctatc ttgccgcagc gcacgcagat aatgaccgca atatcgctct
//
LOCUS       DACEPD010000131         4726 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.140, whole genome shotgun sequence.
ACCESSION   DACEPD010000131 DACEPD010000000
VERSION     DACEPD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.140"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            130..1275
                     /gene="tgt"
                     /locus_tag="IW797_002585"
     CDS             130..1275
                     /gene="tgt"
                     /locus_tag="IW797_002585"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ1225335.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1372..1752
                     /gene="yajC"
                     /locus_tag="IW797_002586"
     CDS             1372..1752
                     /gene="yajC"
                     /locus_tag="IW797_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ1225336.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
     gene            2057..4654
                     /gene="adhE"
                     /locus_tag="IW797_002587"
                     /gene_synonym="adhC"
     CDS             2057..4654
                     /gene="adhE"
                     /locus_tag="IW797_002587"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ1225337.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     AQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         1655 a          834 c         1010 g         1227 t
ORIGIN      
        1 ataaaaaaag gataaaaaaa agaatattaa ataaaataga aggaagccga gggaacttgc
       61 aaaaagcact tgaaacgatt atgatggtgg gagttgtcaa gatattttat atagaaagta
      121 gggaaatgaa tgacgcaacc tgccattcgc tatcgtttaa tcaagaaaga aaaacatact
      181 ggcgcgcgtt taggggaatt gatcacgcca cacggaactt tccccacacc aatgttcatg
      241 ccagtaggaa cattagctac agtaaaaaca atgtccccag aagagttgaa agaaatgggt
      301 gctggcgtta ttttaagtaa tacatatcat ttatggctac gccctggtga agacttagta
      361 gaagaagctg gcggactgca taaatttatg aactgggatc aaccgatttt aacagattca
      421 ggcggctttc aagttttttc attaagtgat atgcggaata tcgaagaaga aggcgtgcat
      481 ttcaaaaatc acttgaacgg ttcgcgtatg tttttatcac cagaaaaagc aatcaacatc
      541 caaaataaat tgggctctga tatcatgatg agttttgacg aatgtccacc attcgatgaa
      601 agttacgact acgtgaaaaa atcagtagaa cgtacgtctc gatgggcaga acgaggattg
      661 aaagctcatg ctaacccaga ccgtcaagga ttatttggaa tcatccaagg agcaggattt
      721 gaagatcttc gtcgtcaaag cgcgaaagat ctagtcggga tggatttccc tggttactca
      781 atcggcgggt tatctgtcgg ggaacctaaa tcagaaatga atcgtgtatt agaattcact
      841 acaccgttga tccccgaaaa caaaccacgt tacttgatgg gtgtcggtgc tgctgattca
      901 ttgatcgatg gtgttatccg tggagtagac atgtttgact gtgtcttgcc tactcgaatc
      961 gctcgaaatg gcacatgtat gacttctcaa ggtcgtctag tagtgaaaaa tgcgaagtat
     1021 gctcatgatt tccgtccaat tgatgagaaa tgtgattgtt atacatgtaa aaactataca
     1081 cgtgcctata tccgccattt gatcaaatgt gatgaaacat ttgggattcg tttgacttct
     1141 taccacaatt tatacttctt gttgaatcta atgaaacaag tacgtcaagc aatcatggat
     1201 gataatttgc ttgaatttag agaagccttt tttgaagaat acgggttcaa taaagaaaac
     1261 gcaaaaagct tctaaaggaa aatcttttaa aaattaagta aaaaactagt gttagcagca
     1321 tttttctgct aaagtgtttt ttgtagatac agaattagtg aggtgaaaaa aatgggaaca
     1381 ttaccaatgc ttgtgatgtt tatcgcgcta ttagcaatgt ggttttttat gtcacgttcg
     1441 caaaagaaac aacagcaaga acgtcaaaac ttgttagata gcatgaaacc aggtgattca
     1501 gtcgtaacga tcggcggatt acatggagtt gtttcagaaa tcgacaatga caaacggaca
     1561 gttgttttag actgcgaagg gatcttcctt gagtacgatc gtgcagcaat caaaacggtc
     1621 aaaccaggga ctgtcgtaac aaatgatgca acaactactg ttgaaacaac agaagaacca
     1681 gctgagacag ttgaagtaaa aactgaaaca acagaaacgc tggaagagac agaagagaag
     1741 aaagaaaaat aagtatcaaa gcttatttaa agacgaggta gggcagaaag atgtcctacc
     1801 tcatttttta ttcaaaaaag aaagagataa gagtatagga tatattgctc aatattgaat
     1861 aatcgggtgt gtcatataaa taataataaa taactttata tatttttcta tacaaacgcc
     1921 tataaataaa gcaactttat atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt
     1981 acatagaaaa ataaatattg tatgatatac atgtgaatga tgtaacaaat aaccatgacc
     2041 cattaggagg ataatgatgg ctaaagtaat ggaaaaagaa aaaacgaaga caattgacgt
     2101 acaagcaatg attgatgatt tggcagaaaa agcaaatgca gcattgaaag aaatggaaag
     2161 ctttgatcaa gaaaaagtgg accatatcgt gcatgaaatg gcaatggcag ctttagatca
     2221 acatatgcca ttagcgaaga tggctgtaga ggaaacaggc cgcggtgttt acgaagataa
     2281 agcaatcaaa aatatgtatg cgtccgaata tatttggcat aatatcaaac atgataaaac
     2341 agttggtgta atcaatgaag atgcccaaaa aggattgatt gaaattgctg aaccagtcgg
     2401 tgtggtttgc ggagtaactc caacaacgaa tccgacttcc acaacgattt tcaaatcaat
     2461 gatcgcgttg aaaacaagaa atccaatcgt ttttgctttt catccaagtg cccaaaaatc
     2521 ttctgcagaa gcagcacgag tagtacgtga tgctgctatc gctgccggag ctcctgaaaa
     2581 ttgtatccaa tggatcgaac atccttcgat cgaagcgact tctatgctaa tgaaccatcc
     2641 aggtatcgcg atcgtcttag cgacaggtgg agctggaatg gtcaagtctg cttactctac
     2701 aggaaaacca gctttaggtg taggtccagg taacgtacca gcttatattg aaaaaacagc
     2761 aaaaattaaa cgtgcagtga acgatttgat cgtttcgaaa acatttgata atggaatgat
     2821 ttgtgcttct gaacaagctg tgatcgtgga taaagaaatc tatgcagcag taaaagcaga
     2881 attccaagca caccaagttt atatcgtaaa accagatgaa ttgcaaaaat tagaagatgc
     2941 agtgatgaac gaaggaaaat atgcggtcaa tccatcaatc gttggacatt ctgcgatgga
     3001 aatcgcaaaa ttggcaggaa tcagtgtacc aaaaggaacg aaaatgctga ttgctgaatt
     3061 ggaaggcgta ggcccagatt atccgctatc aagagaaaaa ctttctccag ttcttgccat
     3121 gatcaaagct aacaacacgg accatgcgtt tgatttgtgt gaaggtatgt tgaatcttgg
     3181 aggattagga catacagcag ttatccattc agaaaacgaa gagttgcatg tcaaattcgg
     3241 tcttcgaatg aaagcttgtc gtattttagt gaatacacca tctgcagaag gcggcatcgg
     3301 tgatatctat aacgaaatga ttccgtcatt gactttggga tgcggttctt atggtaagaa
     3361 ctctgtttct cgtaacgtct ctgctgtaaa cttaatcaat gtcaaaactg tagcgaaacg
     3421 gagaaataac atgcaatggt tcaaattacc gccaaaaatc ttctttgaaa agaactctct
     3481 attgtactta gagaaaatgg aaaacgttga acgtgtcatg atcgtttgtg accctggtat
     3541 ggtccaattc ggttactgcg atacagttcg tgaagtattg gcacgacgta aaaatgatgt
     3601 gaagatcgaa gtcttctcag atgttgaacc gaacccatcg acaaataccg tatacgcagg
     3661 aacgaaactg atggcagact tcaagccaga tacagtaatc gctcttggag gtggttctgc
     3721 aatggacgca gctaagggaa tgtggatgtt ctatgaacac ccagacacat ctttctttgg
     3781 tgcaaaacaa aaattcttgg atatccgtaa acggacatat aagatcgaca aaccagaaaa
     3841 aacacaattc gtatgtatcc cgacaacatc agggactggt tctgaagtaa cgccatttgc
     3901 ggtcatcact gacagtgaaa cacatgtgaa atatccgtta gcagattatg cattgacgcc
     3961 agatgtcgca atcgtcgatc ctcaattcgt catgtcagtt ccagcatctg tcacagcaga
     4021 taccgggatg gatgtgttaa cacatgcaat cgaatcctat gtttcagtca tggcttcaga
     4081 ttatacacgt ggattgagcc tacaagcaat caagttagtc tttgactatt tagaaaaatc
     4141 agtaaaaaca cctgatatgg aatcacgtga aaaaatgcac aacgcttcta caatggctgg
     4201 tatggcgttt gctaacgcat tcctaggtat ttgtcactca gtcgcacata aaatcggcgg
     4261 ggaatacggg atccctcatg gacggacaaa tgcgattctt ttaccgcata ttatccgcta
     4321 taatgcaaaa gatccacaaa aacatgcgat gttccctaaa tatgactatt tccgtgcaga
     4381 tacagattac gcagatattg caaaattctt aggtctgaaa ggcgaaacaa cagaggaatt
     4441 agtcgaagct ttagcaacgg ctgtttatga attaggaaaa tctgtcggca tcaacatgag
     4501 tctaaaagct caaggagtca cacaagaaac actagataca accgtagacc gtatggctga
     4561 attagcttac gaagaccaat gtacgacagc taatccgaaa gaaccattga tctctgaatt
     4621 gaaacagatc atcattgatg cttacaacgg ataagcaaaa gaaagcgtag aaaaaagata
     4681 agaaaataga tgaaaaagaa cttggaaaac gttgtttctc caagtt
//
LOCUS       DACEPD010000132         4616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.141, whole genome shotgun sequence.
ACCESSION   DACEPD010000132 DACEPD010000000
VERSION     DACEPD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.141"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(105..629)
                     /locus_tag="IW797_002588"
     CDS             complement(105..629)
                     /locus_tag="IW797_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1225338.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IW797_002589"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IW797_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ1225339.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IW797_002590"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IW797_002590"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ1225340.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="IW797_002591"
     CDS             complement(2351..3031)
                     /locus_tag="IW797_002591"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ1225341.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="IW797_002592"
     CDS             3157..4026
                     /locus_tag="IW797_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ1225342.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="IW797_002593"
     CDS             4052..4567
                     /locus_tag="IW797_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225343.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
BASE COUNT         1310 a          985 c          851 g         1470 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc ttttta
//
LOCUS       DACEPD010000133         4513 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.142, whole genome shotgun sequence.
ACCESSION   DACEPD010000133 DACEPD010000000
VERSION     DACEPD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.142"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(623..2128)
                     /locus_tag="IW797_002594"
     CDS             complement(623..2128)
                     /locus_tag="IW797_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1225344.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2125..2811)
                     /locus_tag="IW797_002595"
     CDS             complement(2125..2811)
                     /locus_tag="IW797_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1225345.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(3004..4425)
                     /gene="gndA"
                     /locus_tag="IW797_002596"
     CDS             complement(3004..4425)
                     /gene="gndA"
                     /locus_tag="IW797_002596"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ1225346.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1259 a          932 c          743 g         1579 t
ORIGIN      
        1 aaatgtttgt tgtcttgaat tgaatcaaga cgccctacac atttggtttg tcttactttg
       61 ttcagttttc aaaggtctaa tctttttgtt ttaacaactc tttaatctta tcagattcag
      121 aagagaatgt caacaacttc tttgtttatt ttttctaaac gtctttgcca ctcattagca
      181 gcgacttgat tatcatacca tctttattgt tatttgtcaa taactttttc gaagtttttt
      241 tcgataaaat caagctttat tagctatctc atccaaacga cttcgttatt ataacatgac
      301 atcaaaagtg cgtcaacact attttttata atttttagtg acaccttaaa ggaatcattt
      361 agaacaaata ataatatagc aatctcttta taaaattgca atagaaaatt ccctattttt
      421 atttattatt tttaaacacg aatattacag cattttattg ttttttcgtt catctaaaag
      481 atacttacat ttaattaacg gatgttattc tatttaattg ttcgctagtt tttttataat
      541 gacatccctg aatttctcac ttgcttgtat tttgtacctt attctttgtt ggatatataa
      601 aaaaacaagt aaagcacatg ctttacttgt cttctgcctt tgcgatagga ataaagattc
      661 taaaaattgt cccttgccct actacacttt ctgctaagat acgtccttta tagttttcaa
      721 ccagttgttt agcgatagac aaccccagac catttccacc cttcgttcta gctcttgctt
      781 tatccactcg ataaaaacga ttgaagatct tattaaggtc ttctttgggt attccttcgc
      841 caaagtcctg aatcgcgatc tcaaattcat tcagagtgga agaaatagag atatggactt
      901 cttttcgtgt agttgaatac tttactgcgt tatccaatat gataatgatt aattgttcaa
      961 agtgattacg ataaatttgc agggtcactt cttttgtcaa atcatcatct aaagtgatca
     1021 catagtctgg atataatatc ttgaaattat tataaacttg ataggtgact tcttttcctt
     1081 tagttgtttc attcgcataa tgaacatcta cttgttctgc tcttgataag tctagcatct
     1141 cttgaactaa gcttttcata cggctgattt cttgcatgct ggcgcttaat gattcttcta
     1201 atatctcagg atcatctttc ccccaacgat ttagcagact aagatgccct tcaataattg
     1261 caacaggtgt ccttaactca tgagatacgt cttccacgaa ttgctcttgt tgttcaatat
     1321 acaaacgcat acggtcaagc atctcattga atatttcagc taaatctgcc aattcatcat
     1381 ttgtgtcgat ttcaggcatg tggatatctg attgcggatc tttacgtata gtatccattg
     1441 tatctctgag taccttgagt ggttttaaga aataagaaga taaaaagaaa cctaaaatac
     1501 tacttaagat caaagaaaca acttctaaaa caataagagt caataatagc ttatttcgaa
     1561 tatcatagaa agaagataat tcatagaagc tttgcgcata gccgatcttt tctcttgtac
     1621 ctttagaata tataggctgg atagataaaa agccagtttt tccttctagt gtaacgatcg
     1681 ttggaacttt acgtgtagtc tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca
     1741 agttctcgtc caaatcataa acataaagat ccattgatgg ctgccctagc tcagctataa
     1801 aggaatcgat cttcattaag tttccttcga ccagtgaagt atgatctacg acctcatctc
     1861 cgtcatttgt cgctttcgtc agattacgat acgtatttac taatgtcagt tcttcatccg
     1921 agttagccaa acgagaagct acttctgaaa tcgtacgctc tacattgttt ctttcttttg
     1981 ccacaatcag attgatagat gatttatagg taatgacagc gaaaattgta aacacaacga
     2041 atatgaagaa agaactcgca aaggcccact tgattgtcaa ggagggccct ttcagttctc
     2101 ttttttccga ttttttcggc tttatcacga gcgcatcaca taacctgttc cgcgtacagt
     2161 ttggatatag ctttcctccc cgggaacgtc aattttgttg cgaagataac gaatatatac
     2221 gtccactacg tttgtttcaa cttctgtttc gtatccccac actttattta gtaaaacgtc
     2281 acgtgctaat accacgttca cgttttccat taaagtaagc agcaactcgt attctctttt
     2341 tgttagttca attacttcat ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa
     2401 atcgcgataa gtaatagtag tttgtttagc cacattttta tcgccttcaa tatcgattcg
     2461 acgcaacaat gcacgaagac gtgctaataa ttcttcgata gcaaatggtt tgacgatgta
     2521 gtcatctgct ccgtggtcaa gtccagatac acgatcgatc acagaatctc ttgcagtcat
     2581 cataatgatt ggtgtatttt tgacttggcg tacacgtctg cagacttcta aaccgttcaa
     2641 ttctgggagc atcagatcca acaatatggc atcccactca ttatttaatg ccgcatctaa
     2701 accagttcgg ccgttgtagt ggacttctgc gttataccct tcatgcttca actctagctc
     2761 gacaaatcgt gccaagttct tttcatcttc aatgattaga atattgctca tttattattt
     2821 tatcctttct attctactgg ctttttaaaa actctatcat tataccgata atatgcttaa
     2881 aaatctatag tcattttatc atggatagca attttaaaaa agagctgaag tctactcata
     2941 acaatcgact tcagctcttt cgtcctcttt ttattttctc atttcctaac agccgaaaga
     3001 ctattattct tcatggtacc atgagtaatg gaagattcct tctttgtcga cacgctcata
     3061 ggtatgtgcg ccaaagtaat cgcgttgggc ttggattata tttgcaggca gacgttctgc
     3121 tctgtatgaa tcgaagtaag cgatggctga tgagaaagtc ggtacaggta cgccagcttg
     3181 gacagccaag gcaacgacgt ctctgactga ttgctgatat tttttcgtga tttcaacgaa
     3241 gtaatcatct agcaataaat tttcgatctc aggatttttg tcataggcat ccgtaatttt
     3301 ttgcaagaat cgagctcgga tgatacatcc agcgcgccag atttttgcga tttcaccaaa
     3361 tggcaagtcc caatcaaatt cttttgaagc gacacgtaat tgagcaaatc cttgggcata
     3421 actcatgatt ttactgaaat acaacgcttc tcggattttt tcgaccaatt cttttttgtc
     3481 tcccgtgaac tcaaaatcgt tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc
     3541 tttataagca gaaatatagc gggcaaatac agattcagtg atcaatggaa gtggcacacc
     3601 taagtcaagc gcgctttggc ttgtccattt acctgttcct ttattcccag ctgcatcaag
     3661 gatcacatct actactggtt tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc
     3721 agtgatttct atcaaatagc tgtctaattc gccttgattc cattctttaa agatatcagc
     3781 catttcttct actgaaaggt ttaaaatatt cttcattaag tcataagatt cagcaatcag
     3841 ttgcatatct ccatactcaa taccgttgtg taccattttc acataatgtc cggctccatt
     3901 tgggccaata tatgtgacac atggtgctcc gtcttctgct tttgcagaaa tttgctctaa
     3961 gattggtgca actagcgcat aggcttcttt ttgtccacca ggcatgattg aaggtccttt
     4021 taaagctcct tcttcaccac cggaaacgcc tgtaccgata aagttgatgc cagagttggc
     4081 taactcttca ttacgacgca tcgtatcttt aaagaatgta tttcctccgt caatcaaaat
     4141 atcgccttta tcaaggtgag gaagtaactc ttggattgtt gcatctgttg ccggtcctgc
     4201 tttgaccatt agcaagatcc gacgtggttt ctcgatagat tctacaaatt cttcgattgt
     4261 atatgttgct ttgaaatttt tatctgggtg ttcttctact acagcggttg ttttagcccc
     4321 tgtacgattg aataaagcca ctgaatagcc gcggctttct atgttcaagg ctagattttt
     4381 acccataacg gccataccga caacgccgat ttgttgtttt gacatatgaa ctcctcctac
     4441 atacgggatt tctccctaat ctcttacatt atagcttaac acggctacat tgcaaactaa
     4501 agaacaacat att
//
LOCUS       DACEPD010000134         4433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.143, whole genome shotgun sequence.
ACCESSION   DACEPD010000134 DACEPD010000000
VERSION     DACEPD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.143"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(1..402)
                     /locus_tag="IW797_002597"
                     /pseudo
     CDS             complement(1..402)
                     /locus_tag="IW797_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            478..633
                     /locus_tag="IW797_002598"
                     /pseudo
     CDS             478..633
                     /locus_tag="IW797_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            652..1929
                     /locus_tag="IW797_002599"
     CDS             652..1929
                     /locus_tag="IW797_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1225347.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1939..2595
                     /locus_tag="IW797_002600"
     CDS             1939..2595
                     /locus_tag="IW797_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ1225348.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2595..3971
                     /locus_tag="IW797_002601"
     CDS             2595..3971
                     /locus_tag="IW797_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ1225349.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            4101..>4433
                     /locus_tag="IW797_002602"
     CDS             4101..>4433
                     /locus_tag="IW797_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1225350.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1700 a          512 c          787 g         1434 t
ORIGIN      
        1 ctaagtcgga ctcattttcg atcggtttcg cttgataata gtaggtctga cgagaaatat
       61 ttagaatttt gcacatcgct gatatggaat atttatgctt gttggcatca attacttgtc
      121 ttttcgtccg aatatcagcg ccgcttgctt taaaatatca ttctccattt cgagttgctt
      181 atttttcttt cttagtgcga tcaattctgc ttgttctggt gttaagttgt ctctttcttt
      241 gaatgagccc gttgattgtg cttgcttcat ccatttatcg aaagaagaag gcgtaagctc
      301 atactctcga ataatttctg cgcgaggctt accagcgaga tagagatcga cgatttgttg
      361 tttgaattct tttgaatagg ttcttcgttg acgtcttggc atgaaaaatc ctccagtgtg
      421 ttttttatta ttctacacac cttatttttt ctgtctagtc tagtgtagcc gattcaggat
      481 aaggataagt gtattaattg tggaaaatgt aaaaaagttt gccaaatgaa taaagattca
      541 gttgagaatt gcaatcatct ggagtgtatc agatgtggac gatgtaaaaa tgcttgtcca
      601 gtggatgcaa ttagttatgg agtaagaaaa tagagaggag tagttaggaa agtgataaaa
      661 aatgctatag catatattac aagaaagaaa aatagaactt taataatttt tatcatatta
      721 actatagttc tttcttgctt gtattcttgt ttaacaataa tgaaatcaag caatgaaata
      781 gaaaaggctt tatatgaaag ttctaattct tcaatatcaa taacaaaaaa agatggtaaa
      841 tattttaatg ttaatcaatt taaagatatt gaaaaattaa aagaaattga agaaaaaata
      901 attcaatatg atggattagc aaaattaaaa gatgctaaag tagttagcgg agaacaaaga
      961 ataaatagag aagatttatc tgacgaattt aagaatgttg tttcactcga agctacaaat
     1021 aatactaaaa gaaatatttt atttagtagt ggagtattta caattaaaga aggtaaaaat
     1081 atagaagaaa atgataagaa ttcaattatt gttcatgaag aatttgctaa acaaaacaat
     1141 ctaaaattag gtgatgaagt tgatcttgaa ttactagata ttgaaaaaag tggaaaaata
     1201 aaaagtcata aatttaaaat tatagggatc ttttctggta aaaaacagga aacatataca
     1261 ggattatctt ctgattttag tgaaaatatg gtgtttgtag attattcaac aagccaagag
     1321 atattaaata attcagaaaa taatgaaatt gcaaataaaa ttttaatgta ttctggtagt
     1381 gcagaatcta cagacttagc cttaaacaaa ttgaaagagc ttaaaattga tgagtcaaag
     1441 tattttgttg aaaaagattc taatgcgttt gaagagtctt tagagtcagt gagtggaata
     1501 aaatatataa taaaagtaat gacttattct attatgttag gtgggatggt tgttctttca
     1561 ttaatcttga ttctatggtt aagggaaaga atttatgaaa taggtatatt cttatctatt
     1621 ggaacatcta agatacaaat tataatgcaa tttatattcg agttaatatt catatcgata
     1681 ccaagtataa tatcttcctt atttttagga aatgtattac taaaagtaat tgtagatgga
     1741 ttcattaact cagaggattc aatgatttcc ggtggaagtt taataaataa tagtagtttt
     1801 atgtcaaata taacaacact tggacaaagt tatttaatat taataagtat tattgtttta
     1861 tcagttgtat ttgcttcttc attaatgtta atcaagaagc ctaaagaaat cttatcaaaa
     1921 ataagttagg agaaaataat ggatatatta gaaataaaga atgtagcata tagttatgca
     1981 aattctaaag aaaaagtttt gtcaggagta aatcaaaaat ttgaacttgg gaagttttat
     2041 gcgatagtag gaaagtcagg aacaggaaaa tctacacttc tttccttact tgctggactt
     2101 gataaacctc aaacaggaaa aatattgttt aagaatgaag atatacaaaa taaaggatat
     2161 agtaatcata gaaaaaataa tatatcttta gtatttcaaa actataattt aatagattat
     2221 ttatcaccaa ttgaaaatat tagattagta aataaatcag cagatgaaag tatcttgttt
     2281 gaactaggtt tagataaaaa acaaataaaa agaaatgtta tgaaattatc tggtggacag
     2341 caacaaagag tagctattgc tagagcattg gtatcagatg ctccaataat actagctgat
     2401 gagcctactg gtaatctaga cagtgttact gctggagaaa taattaatat attaaagaca
     2461 ttagctaaag atagaaataa atgtgtaata gttgtaacac atagtaagga agtagcagat
     2521 tctgcggata tcattttaga actaagtggt aaaaagttga aaaaagtaaa taaaatgaat
     2581 ttggaggttg aataatgata aaaaatgcat ttgcttatgt aactagaaaa agtttaaaat
     2641 cattaattat catattagtt attttatcta tgtcaacttt aagtctcatt agtttatcta
     2701 ttaaagatgc tacggacaga gcttcaaaag aaacatttgc taatataaca aatagttttt
     2761 ctatggagat aaatagacaa gtaaatccag gaacacctag aggtggggga aatgtaaaag
     2821 gtgaagatat taaaaagata tctcaaacag atagtataga ctcctatgta aaaagaataa
     2881 acagtgttgc agatttagtt gattatgata ttattgaaac tcaagagact cttgcgaatc
     2941 aatcacctga aagagcaaag aattttaaaa gaacagttat gttaactggg gttaacgact
     3001 caacaaaaga aacaaaattt gtatcagaag catataaatt agtagaagga aagcatttag
     3061 aaaatgaaga taaaaataaa atcttaatgc ataaagattt agctgaaaaa aataacctta
     3121 aagttggaga taagataaaa ataaaatcta atttatttga tgctgataat gagaaaggtg
     3181 cagatgaaac agtagaagta gaaattaaag gtctattcga tgggcataat agcggtgtag
     3241 taagtgcagc acaagaacta tacgaaaata cattaatcac tgatattcat acagctgcta
     3301 aagtttatgg taataccgaa gatacagctg tataccaaga tgcaacattc tttgtaaaag
     3361 gtgataagaa tcttgatagt gtaataaaag atcttgggaa attagatata aattggagag
     3421 aatataattt gattaaaagt tcatcaaatt accctgcatt acaacaatct atatcaggta
     3481 tttactcaat ttcaaataaa ttattttttg gctcattaat atttgcagga gttgtagttt
     3541 cattattatt attcttatgg atgaatgcta gaaagaaaga aatagcagta ttactatcat
     3601 taggtatatc aaaattagaa atttttggtc aattcctaat tgagatggta tttatatcaa
     3661 tccccgcatt cgttggttcc tatttcttag ctcaatatac agctgacaag cttgggaata
     3721 atatattgaa taaggttact ggtgatatag ctaaacaaat agctaaacaa tctgcatcta
     3781 gccaattagg tggtggggca gaagctgaag gatttaacaa gacattatca agtttagata
     3841 tcaatgtatt acctaaattc atgatttatg tagttatatt tatgagttta gtacttcttg
     3901 tatcattgat tatttcttca ttcaatatat taagaaggaa tccaaaagaa ttattaattg
     3961 acaataatta aaattttcgg tgctttttag caccaaaaat gagagtgtaa tataaaccgt
     4021 gtaagtaacc atcaatccta ggcgggtggt tgcttcacgg tttttatttt tctaaaataa
     4081 gatcaagagg tgattacacg atggctagaa agaaaagaaa tcctgatgct gagaagttag
     4141 ctgaatccat tttgaatact tatcaacccg aatctgtaga ggatatgcaa gatgttttaa
     4201 aagatgtgtt tggtccctta tttgaaaaaa tgcttcaagg agaattgaat aaccatttag
     4261 ggtatgacgc ccattctaaa gagccaaaga aacatgacaa tcgacgaaac ggttatggca
     4321 ataagacgct taaaacgagc tttggtaaag taaacattga tgttcccaga gaccgggagg
     4381 cttcttttga acctgagttg attcctaata gaaaacgaga tgtctccgac atc
//
LOCUS       DACEPD010000135         4429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.144, whole genome shotgun sequence.
ACCESSION   DACEPD010000135 DACEPD010000000
VERSION     DACEPD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.144"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(401..718)
                     /locus_tag="IW797_002603"
     CDS             complement(401..718)
                     /locus_tag="IW797_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1225351.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(719..973)
                     /locus_tag="IW797_002604"
     CDS             complement(719..973)
                     /locus_tag="IW797_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1225352.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            1332..1736
                     /locus_tag="IW797_002605"
     CDS             1332..1736
                     /locus_tag="IW797_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ1225353.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            1753..2901
                     /gene="tnpB"
                     /locus_tag="IW797_002606"
     CDS             1753..2901
                     /gene="tnpB"
                     /locus_tag="IW797_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ1225354.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(3124..3342)
                     /locus_tag="IW797_002607"
                     /pseudo
     CDS             complement(3124..3342)
                     /locus_tag="IW797_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3608..3802)
                     /locus_tag="IW797_002608"
     CDS             complement(3608..3802)
                     /locus_tag="IW797_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225355.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT         1522 a          760 c          699 g         1448 t
ORIGIN      
        1 ccatagatat attggacttc ctcttttaaa acacaaaaat aaatactacc ttatttaggt
       61 agtatttatt aaaagttacg tcaattttga ttaaaaatat actacttgaa cctctcatga
      121 gtaaagtcac gaaatttttg ggaatagagc gtacttgata tcttattttt tcactaatag
      181 cagtgtctct tattcaccaa gcgatccccg tagttcctac ggttcttaat ttttctctag
      241 actaatgatt gtatctttag accttcggcc ataacatgga gcaaaataaa aaggtactaa
      301 gtcttatata tcaatcttcg agtaaccgac ggttaacaac catatcgagc ggcattaatc
      361 acccacctgt agaggatgag cgactttttc ctaattattg ttaaaagcct agtttcattt
      421 gtttagattg gaaaatacgt cctacaagta tctctttttt ttcatggtcg atccgataaa
      481 ataaggcata gcttttgccg atcacgatcc gatatgttgg gatatccatt ccatagattt
      541 cggaaacttc ctcatgcata gttggaaaat ttttggttag ttctaaagcc ttctggatcg
      601 cacctacaaa tctttttatg ctggctggag aaagagcaag ctcattttcc cattcttcta
      661 ttatcgtttc aaggctggtc tgaaaacttt tagaataaac tagcgtgtac tctttcatct
      721 attcactcca gccgtatttt ccgaaagctt ttaccatttc aatttctgaa actgttaaac
      781 ctttctcaaa gtcttggaaa ctttcttcaa tcaatttgcg atcgtgttct tttgctattt
      841 gagtagtatc taatatgaat aagttgtctt cttgtttggc ttggataatg tctccgcttt
      901 ttaatccaag agagcttgga aggatcactc ccacgctatt gccgatcttt ctaaccttta
      961 gttcttttcc catgttatca cactcctttt agtttttgat gaataaatta taacatgtta
     1021 taacaacaat tgccaacggt gtttcttaag ggattccact ccatattatt attggacaaa
     1081 ataagccact tagaacgctg aaaacatata caaacgatat caaaatacga gtgaactact
     1141 caccatacac ttccgttaac atccatttaa cagaattagg cagaagtcgc ccatcctcta
     1201 taggtgggtg atgaatgccg ttcggtatga gggttaccgt tggtaactcg aagaccgaca
     1261 actaaggcgt attgcctttt tattttgtcc catgttacaa tcagtctata caagaaatga
     1321 gttgatataa catggaatta gatacaaata atcattcagt atttcttctt tattaccacc
     1381 ttgttttagt aacgaaatat cgtagacaag tgattgatga tgaaatatct gattatgcca
     1441 aaactacttt tgaaagaatt tcagaatcgt atcacattac tttagtggag tggaatcatg
     1501 ataaagacca cgttcatatt atgttcaaag ctcaacctaa aacagaattg acaaaattca
     1561 tcaatgccta taaaagtgcc agctcaagat tgataaaacg tgactttcca agagtcaaac
     1621 aatttctttg gaaagaaatg ttctggtcta aaagcttttg tcttctgaca actggtggcg
     1681 caccaattga tgtgattaag aagtatatcc aaaatcaagg aaacaatcat aaatagaaag
     1741 taggtgaact ttatggaaca actaaaagca tacaaattca gaatctatcc aacagaggaa
     1801 caagaaatct tctttgccaa atcttttggt tgtgtccgta aggtctacaa tctaatgctt
     1861 gatgaccgaa aaaaggcgta tgaagaagtt aaaaatgatt cttctaaaaa aatgactttt
     1921 ccgacaccag ctaaatacaa gaaagaattt ccgtttttga aagaaattga tagccttgct
     1981 ttagccaatg cccaacttaa tttagataaa gcatacaaga atttttttcg ggataaatcc
     2041 gttggatttc cacgtttcaa aagtaagaaa aatcctgttc agagttatac aacgaataat
     2101 caaaatggta caattgcttt gattgatagt aaattcatca aagttcctaa attgaagtca
     2161 ttagtaagaa ttaagcttca tagacaacca aaagggatta tcaaatctgc tacaatatca
     2221 cgtcattcta gcggtaaata ctatatttct ttgttgtgta aagaagaaat tatcgaatta
     2281 cctaaaacta attccacaat tggtattgac ttaggtatta tggactttgc gattctttct
     2341 gatggacaaa aaattgataa taataaattc acgtccaaaa tggaaaagaa actaaagcgt
     2401 gaacaacgca aattgtctag gcgtgcttta ttggctaaaa ataaaggtac caatctattt
     2461 gaagctaaaa attatcaaaa acaaaaacgc aaagtagcta gattgcatga aaaagtaatg
     2521 aatcaacgta ctgattttct gaataagttg agtacagaaa tgatcaaaaa tcacgatatt
     2581 atctgtattg aagacttaaa cacaaaaggt atgttgcgaa atcataaatt agctaaaagt
     2641 atttctgatg tttcttggtc aaagtttgtg actaaattac aatacaaggc tgactggtat
     2701 ggacgaaaaa tcatcaaagt agataaatgg tttccgtcta gtcagatttg ttcagagtgc
     2761 ggacataaag atggcaagaa atctcttgaa attcgagaat ggacttgtcc tgtttgtcat
     2821 actcatcatg atcgtgatat caatgctagt atcaatattc tgaccgaagg tctaagacta
     2881 cattctatgg gattggctta gatccaatca tcaagaaccg taggaactac ggggatagct
     2941 tggtaaataa gagacaccgc tgttagtaaa gaaatacgct atcaagtatg ctctattccc
     3001 aagaagctcc cacttcaagc gttaagaacc gtcaggttta gctaagtggt gagtagttca
     3061 cgtaggtatt tttattatat atagatttaa catatgtcgg tcggttgatt gatatagcta
     3121 aagttataac tcaaccgaag ccgattcatc tacacgaata aagttgcgag tgttctcggc
     3181 taattcataa aaattgttat gtgttccata tagcaatact ggaataactt tattttcttg
     3241 aatctcataa gcaactctat acacggtgac cgcatactta aatccattag accaattacc
     3301 tgataaacct ccagtttttt gctcttactt aaatggatca atggcaattt catcacaaac
     3361 ctatagagga tgagtgactt ctgcctaatt acgttaaaaa atattgaaag taactctatg
     3421 tagcaaaaaa tatctagaat ttcatttcct caaaactgat cgatattgta tctaaaaata
     3481 tatactacct aaaataggta gtatatatta aaatatgtgc ttttgttaga aaaatcaata
     3541 cataaattat catatctgta tatgtcattt aggtatttca gttatgttat aatagttttg
     3601 ctgtctgcta ccccggccta atctggcaag gggaggagga gatataatga aatacctttt
     3661 tgattttgtt tttgcaatca agccacttgc agatgtagtg tgcagttacc tctgcaaatg
     3721 gcttgattgc aaaattgcca aaaaagacag taagcctacc cgttaattct ataatataga
     3781 aacgtaaaaa aaaccccatc agcctgcacg ctgatggggt ttggtgatat aatgaaatac
     3841 ctctttgtta ctcataattg taccataatt agtcacgaat gcaaccaata gaaaaaacta
     3901 cttagaacgg tagatcatcg ctaataacaa tgcattgtcc accaaatgga ttttgtgaag
     3961 cattaaatac attgttatta attgtcgctg cttttaacta ggcctctccc cttctattat
     4021 aaaagaagat atatatcatt tcttaattac ctactacctt aaaccaaaat ttaagtacta
     4081 cctaatttaa gtattactta aacaatctaa aaaatagtta tcccaattgt tatgctttta
     4141 taatacgatt atttgtaaca aaatgaatat tatataatta aacactacca tatttaggta
     4201 gtgtttaatt attagcaaaa caaaaaatat ttattctatt ctcttaactt caactaagtt
     4261 atgctgcaca cctttatata aatcatatgc aactcattag ctagacaaac tacaagcatg
     4321 tagttttatc tgttaattat gtactaccta aataaagtag tttataatta gcaagtatat
     4381 atatttaaaa atttaagatt aattttttta tgacttgtac aaatttatc
//
LOCUS       DACEPD010000136         4400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.145, whole genome shotgun sequence.
ACCESSION   DACEPD010000136 DACEPD010000000
VERSION     DACEPD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.145"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(98..1618)
                     /locus_tag="IW797_002609"
     CDS             complement(98..1618)
                     /locus_tag="IW797_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1225356.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(1714..1839)
                     /locus_tag="IW797_002610"
     CDS             complement(1714..1839)
                     /locus_tag="IW797_002610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1225357.1"
                     /translation="MYKRFESGYLQWPRSRKEVLLTGLSIEEKCTLKETKKGAIY"
     gene            complement(1861..2046)
                     /locus_tag="IW797_002611"
     CDS             complement(1861..2046)
                     /locus_tag="IW797_002611"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAQ1225358.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(2030..2212)
                     /locus_tag="IW797_002612"
     CDS             complement(2030..2212)
                     /locus_tag="IW797_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225359.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            2453..2671
                     /locus_tag="IW797_002613"
                     /pseudo
     CDS             2453..2671
                     /locus_tag="IW797_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            3166..3765
                     /locus_tag="IW797_002614"
     CDS             3166..3765
                     /locus_tag="IW797_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1225360.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            3829..>4400
                     /locus_tag="IW797_002615"
     CDS             3829..>4400
                     /locus_tag="IW797_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225361.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT         1402 a          799 c          785 g         1414 t
ORIGIN      
        1 taattcagta gttttgagcg taagcgtgtc atagaatggt acctaaacac aaatagcccg
       61 atttgataca ctcaaatcag gttatttgtg gcttatatta tttacagatt tcttgtatct
      121 gctgattcca tggcaaataa gcgcgtagcg cttcaggtgt catcgaagac agattgggaa
      181 tctctgtaaa aagatgattt aaatatttgc ggatgtttaa cccattcgca acagctgtct
      241 ggataatact caatataatt ccatttgatc tagcgccttt taagctttta gaaaatagcc
      301 agttttttcg accgatcact aactctttga tggcacgctc tgctcggtta tttgaaagtt
      361 ccaagcgtcc atcaagcaac acaatctgaa gtccgtcttt atgattgact gcgtattgaa
      421 ccgcctttcc aagctgcgat tttggcaaat aagcaaaact ctcacaccaa tgaaaaaatt
      481 cagtcatcac aggctgcaac gtttccttcc tcgctacaaa cctttcttct gcgctaagct
      541 tattaaaggt ccgttcgatt cgaaacatct tgtcacagaa gtctaatcct attttggcat
      601 gactttttgg ggacggttgt ttacccaatg cttcaaaaaa ctttctgcga acatgcgccc
      661 aacaataaac attgtttacc ggcagctttc cataggccgc gtaaccatca gtatgcaaga
      721 aaccgctaaa tgattctaaa aaactttttg gctcatcaac ggaacgcgaa tttgcgtgct
      781 cgtagagcac aacttgtttt tcttcgcttt tacctgaagc atataaccag taaaaggtct
      841 tttctttatg agtattcaat acccgatagc ttgtctcatc cgcgtgtaag acctcctgat
      901 tgcttaattc ttgatgaagg caattgtaga ttggctctaa aaccatttca cttaccttga
      961 tatgccaatt agtgattttt ttacgatcaa gagggaaacc aagtgcatgc cattcttttt
     1021 cttgtcgata tgccggcacc tttttaacaa atttttccca aatactctgt gtcacaattg
     1081 aagaggaacc tagactatta ttgatcaacg gtttaggaac aggcgctttt ttgatttttg
     1141 tttctcctaa tgcttcacat tctctgcaat gataggcatg ttggtagatg acttgttttt
     1201 ctatatgggc aggaacatac aatggctctt ctctaagcct tgtcgcaccg atatcatgta
     1261 aagtatgacc acagtcttca cacaaacaat cggcaccttt taattcataa tgaataggaa
     1321 cttcaggcag accttctaat gaaagtgctt tgagtccctt gcgttttctg cgtttaaaag
     1381 aagataccgt aatgggttcc tggctttctt gtccagtgtg ctctggttca ttaaaagttg
     1441 agtcgtctaa taaatcgatt tgcccatcta cgggtggtgt ttcttttttt cgtccaaaca
     1501 agcgtcgatt catttcagca atttgttctc taagaagagt attttcctgt tgctggattt
     1561 caagcaactg tgttaattgc ttgatttgta caagaagttc agattccatt tttgtcattt
     1621 ttacaccacc attctagcga ttttcttaca tctattatac cattttatga caacgaaaaa
     1681 cagacaaaat ccttgattta acaaggtttt cgtctaatat atcgcacctt ttttcgtttc
     1741 ttttagcgta catttttctt cgattgaaag gccagtcagt aagacttctt ttctggaacg
     1801 cggccattga aggtacccac tttcaaatct tttgtacatt aatacaaatc cattttgatc
     1861 ctagtaaaga gccttgaagc gatctttctt cgtaccgcaa aataagaaaa gcgcttgatc
     1921 gtaaacatca aggtcatatt cttctataac gaaagctgct agtccatcaa ttccttttcg
     1981 aagatctgtt tttccacaaa cgataaaaat atgtttcaat gcagttgcat caatgagcat
     2041 tggcattcaa ctcttttaac aaggtataca aaagatattt atccattcca ttataaataa
     2101 atatttcctt atcttccgat ttgattctgc aagctagttt tggcttggtg tcaatttgcg
     2161 atttttgagc cgcacttgaa aattgaactg gtacaatatc ttttttattc attcgttttt
     2221 ctccttctat atttgatagt ttaattatag aagaaagata atcggcgtac gagatactat
     2281 cctatgacac gcttacattt aatttaatat tttgcctcaa attagccaca ctttttatag
     2341 aactaagagg aaaaatgaag ttttcttcct attaatatag ttgtttgaaa tcttaaatat
     2401 agcgttttac gatgtaatgg gaagtatggt gaataaatca actatgttcg tacgaagcat
     2461 ttactcaact aatttaatcg aatctttcaa taaacaaatc aagaaataca gccaccgcaa
     2521 ggaacagttc caaaatgaag agtcgatgga acgtttctta gtctcgtctt ttgatactta
     2581 caaccaaaaa ttcctaggtc gcagtcataa aggtttccaa caggccgaag gcgagctcga
     2641 acaaatgctg agtcaaccga tggagaatta gaaacaatct acagtaggga agaaccattg
     2701 acacaaaatt attgacgctc ccaactaaaa gtgtcaaaat aatgatgaaa tcatttagtt
     2761 cttataaatt agaaaagaga aaataaatat tccatatcta ctttattgga tccgtaacat
     2821 gactaaattc tgaatacttt ttaaagctta tcatttggta aaactgaata taagttaatt
     2881 atatagactg aaaattttga attttcagaa tagaataact taaaagtact gacaaaattt
     2941 tttaaagggg caatattaat gttctgagat aatcaaatcc taccgaaaat atcaaaaatc
     3001 caatgtgaca caatcagatt gtgtctcatt cgagggaatc acacaatccc acgtggctct
     3061 aggcagaatt gaatttttgc ttggggcttt ttccttatat tgattttgaa tgagacttat
     3121 tatatgttat aatgcaatta agtatcattg gagagggcga agaaaatgaa gacggtagaa
     3181 cctattcgag acaaaaaaaa gatcgatgcg atgaaggcaa tcttggcttc gggaaaatat
     3241 ggccaacgaa atttggttct tttttcgatt gggatcaaca ccgcctatcg catttctgat
     3301 ttgcgtcaat tgaagctggc cgacgtgctg gaaatttcac gcggtcgcgt ggtggtcaaa
     3361 gatcgattag cgatgaagga acaaaaaaca gctaaacata attcggtcta tatttccaac
     3421 aaattgcgaa aaacgatttt ggagtacgtt caacaggaat ttcctgatca actccttcag
     3481 caagattttg acggttattt gttccctagt cgaaaaggga cgaatgaacc gctcaaccgc
     3541 caaagtctgt ggcgaatcat tcatgatgct ggcacggcaa tcggattgaa agacattggc
     3601 ccacattcca tgcgaaaaac gttcggttac tttctctata aacaggggac caaaacagaa
     3661 atcattcagt ccttgttgaa ccattcttct cagcgcgaaa cattgcggta tattggtatt
     3721 acacaagaag acaaagatac agctgtaaaa agcttagatt tataatataa aagttaagtt
     3781 taaacatgga tagttttaaa ttagtagatt gagtgttggg aggaaagaat gagtacatta
     3841 tatattatag gaaacggttt tgatttaagt catggtttaa aaactagata cactgatttt
     3901 tcaaaatggg ttgaaaaaag tcatagtgaa atattcgata aaatagacac aatgataaat
     3961 gaaaataatg aggtgagaag tacagctgat gataagatag gttggtttaa ttttgaaagt
     4021 agtttgcatg agttaattca aagtccagtc tttgaagatt atgtagaaga gtctaaggat
     4081 tatctcatag gttatggaca tgaagattgg tcagattctg cccatcatga ttttcaaaat
     4141 atggtttatg attatgttgt ttctctagaa gaattacaac aacttttcaa ggaatggata
     4201 cgcaaagaaa tacagccgac aatagaacat aaaattccaa aataccaatt ttcgtccgat
     4261 gtactattct ttacattcaa ttatacagat gttttggaaa ttctatacaa gcaaccgaaa
     4321 gaaaacatat atcatattca tggcagttat gacgatataa taatcggcca ctccgagaat
     4381 tttgacaact ttataggaga
//
LOCUS       DACEPD010000137         4334 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.146, whole genome shotgun sequence.
ACCESSION   DACEPD010000137 DACEPD010000000
VERSION     DACEPD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.146"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(127..357)
                     /locus_tag="IW797_002616"
     CDS             complement(127..357)
                     /locus_tag="IW797_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ1225362.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(587..1405)
                     /locus_tag="IW797_002617"
     CDS             complement(587..1405)
                     /locus_tag="IW797_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ1225363.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1566..2255
                     /locus_tag="IW797_002618"
     CDS             1566..2255
                     /locus_tag="IW797_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ1225364.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2269..3771
                     /locus_tag="IW797_002619"
     CDS             2269..3771
                     /locus_tag="IW797_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1225365.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3784..4260
                     /locus_tag="IW797_002620"
     CDS             3784..4260
                     /locus_tag="IW797_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ1225366.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT         1440 a          627 c          773 g         1494 t
ORIGIN      
        1 attaatataa aaatcaaaag gatcataaac ttttaacaag tgatgaaatg ggtggaaatt
       61 gattcttata gacagtttgt ctcataacat tgaagtattt tgcaatttta gcattgaaac
      121 acttttttaa tcttcaacgt tgttcttata aacaagatga ttgggaatct tttgaattaa
      181 cactgtatag cttagaacac cctttataga aaacattcgc ttagcaatct taataattcc
      241 ttgtgcccga tgtcgtttag attcagtagg ttcctgctca tcgatcatgg aaaaaatttg
      301 aaggatcaga tccttaataa atttatttaa gagaggatga tccattgctt catccatact
      361 cgataaacta gtgataatga gctacacgtt tttctttcaa gttgatcaat agcttccacg
      421 ataaaaatat ctttagataa aatcaagaac cgtaggaact acagggatat ttggtcaata
      481 agagccaccg ctattagtaa aaaataagct atcaagtatg ctctcatccc aagaattgcc
      541 tacttcaatc gttaaaaacc gttaggttta gctaagtggg ggtagttcac tcaatacttt
      601 tatcaataat agagcgcaaa actttttctc ttaattcaac tgaatcaata ttattgtttt
      661 gttcataacc atgaactaat agatcacaaa tataaagttg tgatattttt ccagctaaag
      721 atccaccatt aagaaattct tcaatggctg tttgtaaaac taagtctgca gactttccta
      781 taggtgaatg aatatagtta gttatagcaa taatttttgc gccatttttt ttagctattt
      841 tcaatgaatc ataagtatct ttcgttttcc cagataaaga aaaaatgatt actaaatcat
      901 taactgttaa taaagaagcg acttgggctt gaaaatgagg gtctagtaca gcttttgctt
      961 gaactcctac gcgaagaaac atattttcta aatcaagact agtatttcca ctagatccca
     1021 ctccaaatat atatatgttt tttgattgag tgatcatttt aatggcttca ttcaattttt
     1081 cttcgtctaa aagcctaata gtggaataga gagcttctgt taaactatta gcaatttcat
     1141 catagtactt tacactagtc ggatgttctt ttttttgact aaagtcttcc tttgcaattt
     1201 caatttttaa atcagaaaaa ccagaaaaac caattttttg acaaaagcga ataatagtag
     1261 catcgccaac ttttgtgttt ttcttaatat cactcatcgt actataaata atagttttcc
     1321 cagaatttaa aataaaatca gcgacttttt tttcagactt cgttaaaaat ggataatttt
     1381 ctttaattaa atagatataa ctcatacaaa ctcctctaca atttcaactt ttctccatta
     1441 tactccaaaa taataaaaaa aactcctaaa tactccattt ttatctttac ttttatgaaa
     1501 gcggatgcta tattgttttt ggagttgtta tccaaaaata ataaaaaatt ggagtgttta
     1561 ggataatgga taaaaagagg tttttagatc ttatttcaaa aggtttgatt gtttcctgtc
     1621 aagcattacc tggtgaacca ttttacaaag aggaagaaag tttaatgcct tattttgctt
     1681 tggctgctca aagggctgga gcagttggta tacgtgcaaa ttctgttcga gatattattg
     1741 aaattagaaa gtgtgtatca ttaccaatga ttggaattat taaaagagat tatccaccag
     1801 aatctccatt cattactgcc actatgagag aagtagatga attagtagaa accggtgttg
     1861 aggtaatcgc tcttgattgc actcttagaa atcgacatga tggactatct ataaattctt
     1921 ttattactaa aataaaagaa aaatatcctc atcagttatt tatggcagat atttctacat
     1981 ttgaagaagg caaaaatgca tataaagcag gaatagattt tgtaggaact actttaagtg
     2041 gatatactga ggaaagtaaa aaacaagatg ggccagatat agatttgatt aaaaaattgg
     2101 tagaagaagg gatttgtgta attgctgagg gaaggattca tactccagaa caagcaaaag
     2161 agataaaaaa aataggagtt gcaggtattg ttgtgggtgg tgctattact cgaccacagg
     2221 aaattgcatc aagatttgtt aaagcattaa gttaggggga gaaataaaat gtttaaaaaa
     2281 ttttcacaat taggacgcgc tttcatgtta ccaattgcta tattaccagt agccggatta
     2341 ctattgggac tgggaggagc gttaacaaat gagagtgcta taaatgctta tccattttta
     2401 gatcaaccgt ggttgcatac aattttaagt attatgagtt atgctggaaa tgcagttttt
     2461 gcaaacctag cactaatttt tgcaattggt attgctgtag gacttgcaaa tggagataaa
     2521 ggaacagctg gtttagctgg tggagtagct tatctagtat atactgcaac tattagtggg
     2581 tttcttgaac ttttttcagc aaaagatgca acactagata caggtgtagt gggatcaatt
     2641 gttgttggtg gtactgttgc atttttacac aatcgttata gaaaaattga attaccacaa
     2701 ttcttaggtt tttttggagg atcaagattt attccgattg tttctagttt agctgcaatc
     2761 attattggtt ctttctttta tttaatttgg ccaccaattc aaaatggttt agtagttgct
     2821 ggtgaacaaa ttgcccaaat gggtagttta ggaacttttt tatatggttt tttattgcgt
     2881 ttaacaggtg ctgttggact acaccacacc atttaccctt tgttctggta tacatcttta
     2941 ggtggtaccg aaactgtagc tggatcaaca attgctggag cccaaaatat tttctttgcc
     3001 caactagctg atccaaacca cactggtctg tttacttatg gtactagatt ttttgcagga
     3061 agatttgcaa caatgatgtt tggattacca gcagcatgtt atgcaatgta tcgtgcaatt
     3121 cctaagaaaa atcgtaaaaa gaatggtggg ctttatttta gcggggccct tacatcattt
     3181 ttaactggaa ttactgaacc agttgaatat atgtttttat ttgttgctcc ttggctttat
     3241 gtgattcatg cattcttaga tggattatct ttttattttg ctgatatctt aaatattcgt
     3301 gtagggaatt ccttttcagg tggtttgatc gactttttat tattcggtgt acttcaaggg
     3361 aatgacaaaa ctaattggtt aaaattaatt ccttttggta ttgcatgggc atttatatat
     3421 ttctttgttt ttacattctt tatcaaaaaa tttaaggtag ctattcctgg aatggaagaa
     3481 gaatcagaag ttcattctga aaaagtaata aacaaaaata catcattaca tgaagagtct
     3541 cttatcatta ttgaagctct tggtggaaaa gaaaatattg agtctgttga agcttgtgca
     3601 actcgtttgc gtgttgctgt tacgaatggg gagattattc aaaaacaaac aataaaagac
     3661 actggtgcaa ctgctgtctt cgaagtgaag ggtggcatcc aagctgtttt tggagggaaa
     3721 gctgacttat taagtcaaga aatcaatcaa atattaggaa aagataatta ggaggttttt
     3781 aatatgtttg aaattttcaa aaagaaaaaa caagaaactt tttatgcacc ttgttccgga
     3841 aaaatcattt ccatcacaga agtaaatgat cctgtctttt cacaaaaatt actaggtgat
     3901 ggttttgccg tcatacctga tggttcaaaa atttatgctc cattggctgg tcgagtaaca
     3961 aatatatttc ctactaaaca tgctattaat ataaaaagca actcaggcat tgattatttg
     4021 attcatattg gaattgatac agttgaatta caaggagagc ctttttcgat ttgtgttcaa
     4081 gaaaatgatg aggttactcc tgaaacctcg ttggttgatg tggattttga caaaataata
     4141 aaatctggta aagatacttc tgttatagtt gtttttccgg agaaaaatca gttcaatgat
     4201 ttaataatag ataatggtcg aattgctcat gggaattcat gtggaaaaat cactttttaa
     4261 aaaagtgtat ataaaaagct ataaaataag ctaaatagga tatttgtcaa atgaagttgg
     4321 tgaacagaaa gaac
//
LOCUS       DACEPD010000138         4219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.147, whole genome shotgun sequence.
ACCESSION   DACEPD010000138 DACEPD010000000
VERSION     DACEPD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.147"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            627..3266
                     /locus_tag="IW797_002621"
     CDS             627..3266
                     /locus_tag="IW797_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ1225367.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3434..4132)
                     /locus_tag="IW797_002622"
     CDS             complement(3434..4132)
                     /locus_tag="IW797_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ1225368.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIAAILLIMIPI
                     ILFATTIYDGSASVSDATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVIGLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
BASE COUNT         1278 a          805 c          911 g         1225 t
ORIGIN      
        1 ttcttctttc tgttctgtcc aaattattct aagatttctc tttaccaaca tcatttgaca
       61 aagagccaga aaaagccgag ctctcgataa gctcggcttt cttgtacctc ggaaggattc
      121 aaataaacag aaatgattgt aactttccat catacatgca aagcacggtg ttgaatttct
      181 tcatgttaaa caaaaatttt gttttcgtta catcttcaac atttgaaaca tctcttgttc
      241 ctattcaatt ttcagacgtt tacgttattc aattgttacg tatcgcatta cttcttgttt
      301 cggatacttc gtgatccaaa catccatacg cattcactat ttataggttt gtactctaca
      361 cgcatgtctg ggtgaaacct gtacttctaa caactttatt tgcttacact gttctatcga
      421 gaaattcttc tgtctatttg atggtatcct ccttccttac atctatataa tagcatcctt
      481 tggtagcgtt tgcaaatata agcactttaa attttacgct ttcttaacaa ataatcgata
      541 agccctttat ttgaagtaaa taaaaaaagg atgttatagt aaaataagta cctttttttg
      601 tacgaagatt ttaaaggagg aataggatgg aagaatacaa gcagacaata gaagaagtaa
      661 agaaggtaac agaagcagat ccagatactg ggttgtcttc acaagaagtg gatgaacggc
      721 gaaaaaagca gggattgaat aaatttgatg aggcgccgaa agaatcgatg atcaaaaaat
      781 tctttcgaag cttatccgat tttacaacga tcatccttct tgtagcagcg gtgatttcct
      841 tttataccgc atttgctaca gaacatgggg atttatttga aggactgctg atcatcgcta
      901 tcgttgtgat caactcggta ttggcgattg tccaagaagg aaacgcagaa aaagcactgg
      961 aatccttgca agatatgaat aaacaaacgg caactgtcat acgagacggc aaagtgacca
     1021 ctgttgagtc agagcaactg gtagtgggtg atattcttgt tttagagtcc ggtgatgcta
     1081 tttctgccga tgcccgtttg atcgaagcat cacaattacg tgtagaagaa tcggcgttga
     1141 ctggagaaag tgaagcagtt gaaaaagatg cagcatttat tgctgaagaa gatgagtcat
     1201 taggtgacca acttaatatg gtatttaaag gatgtacggt tgctgcaggt cgtgggaaag
     1261 cgattgtcac tgccattggt atggctacag aaatgggtaa gatcgcggat cttttgaacg
     1321 aaaatactat gcaaaaaaca cctttacagg tccgattgaa tcagctaggc aaacgaatca
     1381 gtatgatcgc tttagcggct gcggctttag tatttgtgat tggtgaactg caaggagaac
     1441 cactccttga gatgttcatg acttcgattt ccttggctgt tgcagctgta ccggaaacct
     1501 tgactgttat tgttacgttg acacttgcat acggtgtgca gaagatggcg aggaaacatg
     1561 cgatcatccg acagttgcca gcagttgaaa cgctaggaac cgctaatgtt atctgctcag
     1621 acaaaacagg aacgctgaca caaaatgaaa tgcgcgtccg ccgggtatgg agcaaagaag
     1681 atgaagtaac gaatatcgaa gattcaatga cgaacagcgc gatggaaata ttgaaaatgg
     1741 ctgcactatg tacagatgtg acggtcgagc aagaagatga cgaccttgtt atcaaaggta
     1801 atccaacaga agtagccatc gtaagggctg ttgaagagaa ttatcataca aaagcagaat
     1861 tagaagaaaa atatccacga gtaaacgagc ttccttttga ttctgaacga aaaatgatga
     1921 caacagttca ccaaatgggc gaaaaataca tctcaatcac caaaggagca tttgatgttc
     1981 ttgctccgcg ctttagttca ggcgatgtag aacaagcggc gatcgttaat gatcgctttg
     2041 gtaaacgggc actgagagtg attgctgttg gttatgctac ttatgacgaa gaaccgcaag
     2101 atatttcttc tgaagcactg gaaaaagact tacgtttgat tggtctgatc ggaatgatcg
     2161 atccaccacg tccagaaagt aaaggagcaa tcaagcgagc gaaaaaggct ggcatcaaaa
     2221 cagtcatgat cactggagat catgtggtaa ctgccagtgc catagcgaaa gaattaggta
     2281 tcttgaatga tccgtcagaa gcactctcag gatcagaact gcatcaatta tcagacgaag
     2341 aattagatgc gcgtgtcaaa gctctctctg tctatgctcg ggtaacgcca gaagacaaga
     2401 tccggatcgt caaatcttgg caacgtacgg gtgcagtcgt agcgatgact ggggatggtg
     2461 tgaatgatgc accagcgttg aaagcaagta acgttgggtg tgccatgggg atcacaggga
     2521 cagatgtggc gcaaagtgcc gcagatatga ttttgacgga tgacaacttt gcgacgatcg
     2581 tagatgctgt tagtcaaggg cgttcagttt atcagaatat tcgtaaagca atcaactttt
     2641 tactaagctg taatatttcg gagatcttta ttgttttgat tgcgatgctg ttaggatggg
     2701 gagcaccatt tacagctgtg caactactgt ttgtcaatgt agttgctgat ggacttccag
     2761 gatttgcttt aggaagagag ccagcggaac acggcatcat ggatcagcca cccattccga
     2821 aaaatgaagg gatctttgct cgaggtttat tgcaaaaaat tgccataaat gcaggggtct
     2881 tcaccatcgt aaccttgttt ggttattatt taggtagtta tgtcgatact atcagcccat
     2941 gggtagacgc aagtcagcat gtcggtcaaa ctgttgcatt cttgatctta gcttattcat
     3001 cgattctgca tgtgttcaat gtacgcagca gccaatcgat cttcaaagtc aatttagcaa
     3061 ccaataaagc tttggtagaa atggcgttac tggcattagc tattacaacg gctgttgctt
     3121 tgctgccatt tacgcaagaa ctattcggcc tagtacacat cagtttgaat cactggttct
     3181 tggtagggat tttatctatc gtaccaatcg cggtgaatga actgatcaag ttccatcgat
     3241 taccggaagc agaagaagag gaatgattaa ctgtttctaa gttgattttc tttttactgg
     3301 aactatacaa aaaggaggtg tgacatgaat gttgtcacac ctcctttttg ctaatgggct
     3361 gaatgtttct tttactgcct ctcgataaac ggtgttctcc taccaacccc tcggtcacaa
     3421 gtcacactct cttttaaata tcttttggca attgttcata aaatgctgct tttgttgcgg
     3481 aaatgtatgg gctgagccat agataaccaa taccagcagt tgctaatgct aagatatgcc
     3541 agccgataaa tgaaagatct aaccagaaaa gttgtccttt ataaccatcc atcaattttc
     3601 gacttgcagt gatcgtatcc agtactttag gtgtttgtcc tgtttcttgg acgatatcgt
     3661 agtagataaa atagctttga gaataagaat agcttttaat gattccagga ataactaata
     3721 gcaaacccca taatgttgta aagatcgaga tcagcacagc taagccgatc actccgccga
     3781 taaacaaacc tttgaatcca cgaaatgcat ctttgaatgg ttcgatctga tctttttgtc
     3841 cacgtaaaat atcaagataa gtccaagaaa taccgctcat gaacaaagcg ctaatgatgc
     3901 tggaaataat tccgccaccg cctcctgaac taccgcctcc tgaagttgtc gcatctgaga
     3961 cgctcgctga tccatcatat atggtggtgg caaataagat aataggaatc ataataagaa
     4021 gaatagcagc aataccaatc aatgtaggga ttagatttag taaaacagct tgtccccaac
     4081 gtccttttag actctctttt gcttggcgtt ttaattctgc agaagattgc atgatttttc
     4141 cctccttttc tttaataaca ccaatatacc atgtatcaaa aaatatttct gtcataatgt
     4201 ttttctcttt tttgttatt
//
LOCUS       DACEPD010000139         4199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.148, whole genome shotgun sequence.
ACCESSION   DACEPD010000139 DACEPD010000000
VERSION     DACEPD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.148"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(2..1222)
                     /locus_tag="IW797_002623"
     CDS             complement(2..1222)
                     /locus_tag="IW797_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1225369.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1242..1847)
                     /locus_tag="IW797_002624"
     CDS             complement(1242..1847)
                     /locus_tag="IW797_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ1225370.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1896..2828)
                     /locus_tag="IW797_002625"
     CDS             complement(1896..2828)
                     /locus_tag="IW797_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ1225371.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLSNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3253..4110)
                     /locus_tag="IW797_002626"
     CDS             complement(3253..4110)
                     /locus_tag="IW797_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1225372.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKNDTID"
BASE COUNT         1191 a          849 c          692 g         1467 t
ORIGIN      
        1 tttattttac agaagcactc gaaactgcat ctactgcagt ttcatcgtgt gtgctggcgc
       61 tggttactgc atctgttttt ccaccatgaa cagatgcact tgaaaccgca tcttcatttc
      121 caacaagttg ttgacccgtt tgttgtaaat accactcaat gattggtttg aaatcaagaa
      181 tgtattcatt gattccagca tactctccat tttcatcatg cattgcttgg taattatgca
      241 caatatattt ttctggcact tgttttggta catgaacacg taccgcatct gttgctccgc
      301 ttcgcagttg ttgaattacc cactctacat ttttatacga tttttctggg tgacagttgg
      361 ctaatggatt ccctacctga ccgggcacac gagcagctaa catctctttc gcttcttggc
      421 gcaaattata atataaaaat tggttattat tatctgcaaa agttaattcc atcggcatcg
      481 aatctaaaaa gtaattgatt tgatccactg tcaataaccc acgatttaat ttgacgtaag
      541 tatttccaga aacggcaccg actgcttcag aagctttttc caaccaatga tcatctgaca
      601 tatcaacatc gggtattgtc gtagcaattg ttcctgtcgc atgcaaatct tctggtggaa
      661 gctttttagg ccctgctaag gtagacacga ctttaatgaa ttttacaaaa cttgctaatg
      721 tactgcctaa aaagtcaaca gttcgtggat caatcaagcg atcttcttgg tcaaatgctt
      781 cttttgcttt tcccaataaa aattcatttc cgggcatcac taatgcattc acgcctggtg
      841 cgtccaaaat ttggcgcaag tgtaattgcg cgcgtgaagt tccttgatcg tagtaagaag
      901 cacccacaac cattacaggt ttattttcaa gtggatggat tttaaatgat agccattcaa
      961 gtgcactttt caatcctgct ggaaccgtat gattgtgttc tggcgtagca ataattaccc
     1021 catcagccat taagattttg cgatttagct gttgaatagc aggactattt gtttgatcat
     1081 catcttgatt gaacagaggt aaatctttga tttcaatgat ttctaaatta aatagttcat
     1141 gatattgttt ttggataaat gttaataatt tacggttata tgaaagttca gctgctgatc
     1201 cgacgatacc tattaatttc atctttgttt gctcccctcg attaaatcgt ttcccatgaa
     1261 aagctttcgg cttcacgttt atttgcttgg tgcgcatgtt gcaattgact agtgattgtg
     1321 acaaagagta agaaatcttt gaatagttca cgtaattttg cgacttgttc ttcatcaatg
     1381 agtcgtcctt gctcatcaaa ggcttggagt gaatgaccca ataaaaattc tgagcttggc
     1441 ataattcgtg cctttaactc tggtgaatcc aatatttggc gcaaatgtgc ttgtgcgcgt
     1501 gaagaaccta acgtgccata agacgctccg gtaatcatca ctggtttatc cacaaacgga
     1561 aaaataccat atgaaagcca atttaatgcg ttaatcaatg aagcaggaac cgcatggtca
     1621 tactctggtg tgctgatgat tacgccatct gcttgttcaa tttttccagc aatttcttta
     1681 acaacatttg gtagttctgt gttttcaggt ttgttaaata gtggcagctc cttgatttcg
     1741 acaatttcga tttctgcctc atttacaaaa taatttttca taaactgtaa taattcacgg
     1801 tttgttgacc gcgttgaatt tgttcctact aatccaataa atttcatctt cattctcttt
     1861 cctttctaac atcgatttct gactgttcgc acgtactaaa ttttttcaaa tccttttgta
     1921 tacaagaatt gattctcttt tgttacaact gctccctcga tacccggctg ttcttcaagc
     1981 gctttcaaca cttctaaaga agatgctcca aataaacgtg tagtccagat ttctccatct
     2041 acagaagaat cagacaaaat cgtcaggctt gctacgtcat ttttaactgg aaagccagtt
     2101 ttcggatcaa tgatgtgatg atacactgac gaaccatttt gtagataacg ttcgtagatt
     2161 ccagaggtaa ctaaagattg gttgcttagt ttaaccaccg caagcgactc ttcacgtgaa
     2221 cctttaggat ttcgcaaacc aatacgccaa gcaccatccg aacgtcttgg tgattcacca
     2281 aaaacaacga tatttcctcc taagttaact aaagcaaacg ctgcatttcg ctctcggcaa
     2341 tgtgtaatta atttatctgc aatgtaacct tttgctaacg cccccaagtc aatcatcatt
     2401 cctggtttgg ttaaaaaaac ggttttcatt ttttcatcta attgaatttg gtgtggatcc
     2461 gttagcagaa gtaactcagt tatctctgct tttgttggaa ccaatgcatc ttcaaatcca
     2521 atgtgccatt tttgtacaac cggaccaata gcaatattca gataactatt tcttgcacaa
     2581 ctatgtgctt ttcctaattt gatgagctca aaaagttcag gatgtacaat cacagcttgt
     2641 cgtccagcag cttgattgat ggccattaat tcagaagttg catcatgtgc gctgaagcga
     2701 tgttcataaa cgtgcaacca ttcgcctaac tctgctagta atcgactagg ctcttcatgc
     2761 ccaacttgta atgtaattgt tgtccccatc atttgccaga ttttcgtttc ttcgatcacc
     2821 tgattcattt tgtaccttct ttacttgaac tacggattac atgagtaatt agtttttatt
     2881 tttgttactg ttttcacaac cagatgatac gcctgctaga tttatttgtc aaccgatttt
     2941 atatattttt ttatataata aggtaatttt aatataaaat tttatattgt aaaaaaacaa
     3001 ataagggaaa caaacaacac atttttgtgt gttgcttatt tccctctaac tatcgttttc
     3061 tatttgatgg ttaacctact taaaataaat actgcaacga caaacggaga tcaagcatgc
     3121 tttgtaacgt attttacgag aattacgact ttattttatt actctatctt gctgccatac
     3181 ccgtctatcc ctaatcatct ttggtgaatt ttttcaaagt tagcataaca aaaataagta
     3241 ttttttcctt cgttaatcaa tagtatcatt ttttttcata tgatttaatt catcaattaa
     3301 ttttttgttt ttattaattt tagcaaaagc tagtgcatct tgaatcaatt cgagcacttc
     3361 tgccttcgac ttatttttag cctgtgcgtt cttcgctaat tgggcagcag ctcgtgctaa
     3421 gtaataagtc aaatggttat ccgaacaaat atcgattgcg taatttagta gtgcatcact
     3481 tgtttccaaa tcaccgtctt gcgcaaaaaa aacaccacaa tgaaagacga tatttaacac
     3541 ccgccagata tcttctgttg tacgaatcgg atacgtataa attttttcaa acactcgatt
     3601 gaaataaact tctgcttttt ccaactcttt ttctctagca tagaccattc caatacctgt
     3661 atatgccaat aaacgaaaca acgtgttggc tgtttctgcc atcaacaact gatcaaaatg
     3721 gaacaacaca tccgtaattg gtgcattcgt aaaaatcatt tcaaatcctt ttagatagta
     3781 gtaacgtgca atcagctcag actcgtcaat taccttggga tcaatggctg ctaaaatctg
     3841 tgtcaattgt tcatattcac tcgtaatcag tgaaaactct gctttttcta gctgttcatc
     3901 aagttgtgta ttgatcacgt tcatcgaaga aaaaagttca actaacggaa gattgattcg
     3961 attacataat ttgattaaaa tttttaacga agggacttga ccattgttct caaatttact
     4021 taatgttgct tgtgtacaaa ttccttcaga cacttctttt tgagataagt tggcagcctt
     4081 tcttgccgta ataaattttt caatgttcat tattttctct ccaatcagac ctcacacgaa
     4141 aatcatttct gtctcgattt tagcatgttt tctttgggaa tgctttaatt ggttaagat
//
LOCUS       DACEPD010000140         4176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.149, whole genome shotgun sequence.
ACCESSION   DACEPD010000140 DACEPD010000000
VERSION     DACEPD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.149"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            612..1424
                     /locus_tag="IW797_002627"
     CDS             612..1424
                     /locus_tag="IW797_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225373.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
     gene            3059..3613
                     /locus_tag="IW797_002628"
     CDS             3059..3613
                     /locus_tag="IW797_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ1225374.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            3867..>4176
                     /locus_tag="IW797_002629"
     CDS             3867..>4176
                     /locus_tag="IW797_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225375.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT         1543 a          516 c          661 g         1456 t
ORIGIN      
        1 atggcctttt tacttttaca caattataag gactttatcg aaaaaaagca gttatttgtt
       61 aaaatcattg atattattaa taataagtat tttttgaaag gggaaatata aaaggttttt
      121 gaaaataaat aaaaaatagt attacttgtt ctattgctag ttattgctag tctttattct
      181 tttttgtttt ttagggatgt acaaaagatc tcaaccacag gagacattgc ttttcacttt
      241 tctagaataa aatgcttgtc ctcagtattt tctggtccaa taaattttac cacatttaat
      301 agctatggcg gaggcgtgaa tttttttatc catatctaac atctttccag ccgtaatatt
      361 ttactggaca acaaacaatt tgattctaag ttatatactt actatacgtt gggatactga
      421 atatctgtac aattctagtg agctatcaat atggacaaaa gaatagatgc agccttctta
      481 ttcagctgtt tgtatagctt ttatggattg atatatatta gcgtactgca atttcagaat
      541 caattgcgtt gacgattgta ccagttgtct tgtattacac atatactctt ctttatgaaa
      601 gaaaaaactg cgtgattctt ttagctgtca gtatgagttt gctcatctat acccatattc
      661 tctccacctt tatgagtgtg attgttattg gcttcttgtt tatcggctct cttttctcta
      721 agcggaaaga gagaaaagaa atgtcgaatc tgatatcaaa tatgataaaa gctgttggtt
      781 ggaccattct tttaacagct tacgtttggt atccgatgtt acaacaagtc ctacatcaaa
      841 aaattaatcg tccttttcgc acaaaacttc aagaaagggc attaaacatt tctgatagtt
      901 taatcggtgc agtaaataat gatttagtta catttacaat gggaacaatt ggtgtggttt
      961 catttattct tccacttttc tttaaaaaaa aatttgccaa taaagaaaag attattaatt
     1021 tttgtgcagt gaccacttgg ttcttatcaa cgaatctttt tccatgggaa tttttacaaa
     1081 aaacacctat tcaaatcatt cagtttcctt ggagaatctt gggttttcaa gtgctttttg
     1141 gttcattgat cttaattata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
     1201 gtagccttgt aggaatagtc cttcttattt ttacagtgac tgtagcaaca gaagcgaatt
     1261 attcgcagaa aattcaatcg tataaaggtc gtttaattat gaccaaaaaa gatgttacac
     1321 tttatacaac ctcgagaact ggaggtcttt atgactatgc accattagat gcattgaaat
     1381 ataaagatca tttaaagaaa catgaagtaa aagtctatga ttgatgggag aatgcatctc
     1441 ataaagcaca taattctaaa ataatctata cagtaggaga tgcgaatgag caaaatatca
     1501 cgttacctgt atttaattac tgggaacagt tatgaaagta aatgggaaaa agagcactat
     1561 agaaaatgac aatggtttag tgtctattaa aggtacggga aaaagaacta tagttgaggt
     1621 ttcatctacg tatccaacta cgatgattct atctttatta attagttcaa taacttttat
     1681 attcttattt acacgttatt taataagatg ctgcggttaa atgaaaatag attagataga
     1741 tattataaat atgttgattc tattttcata gcaatggaga aaatacatta gcaatatata
     1801 aaagaaggta tcaagagtca cggtgaattt attccgaaaa gtgaaaagaa caaatataaa
     1861 atttaataaa aatatagaac taaataggaa aacgtagacg taattttcta cgttttcctt
     1921 ttcatatata ttaaatatta cggaaattta tataaactaa tttagtgttt tgtttgtaat
     1981 tcctaatttt gctccacttt tagataattt ttaggtctat ttaactcatc agacattcta
     2041 tgctcatttt ctccaaattt gtttcaactc tttcgtactt aattttgcgt atttgaaaat
     2101 tcttctttta gagtactttt attacgatac tagtatgata tatatctata gcaaacagtt
     2161 aaaatcttcc taaaataagc gaatagcatt cctaaaacct ataaaatttt tagatgaagc
     2221 gacaagtgga caattaataa actacttgaa catgaaatta tggatcacct ttcaaattta
     2281 gaaaatggaa ttcttatact tattgcgcaa catttattga ttgcaaaatt atgtgacaaa
     2341 aagaactagt agaagaagga ataatggctt gataataaag tactacctat taaaggtagt
     2401 actttattta atttagtata aattaacata ctactatatt ttaagtttca tattatataa
     2461 attaataata tactaccatt ttttggtagt ataatttaaa ggtaataagt tattctttta
     2521 ttactatgtt tgggttttat agcaaggaaa gtttaataag agtttggacc tagcaaacaa
     2581 tattaatgag gtcattaaat cttattatct ttagtgatat aacgaaatat ctacacagac
     2641 aaaattatga tcttgcgtaa agttataaaa tattaataaa aatattttat taatgaaaag
     2701 cttggtagca cggttattca tatgctaatt tatttcactt tatagtaagg ggagttctta
     2761 gtatattgtc caaatttaaa tttcatataa tttaattatg taaattagct attctaagaa
     2821 cttctacgga taactgcccg tctaaaattt aattaagagc ttagaatctt catataagta
     2881 aggttaattt ttttgtacaa aatagtcgga atattctgag ttactgaaat gatataaaat
     2941 cataaactaa accccttgat tttcttcact tttaacagag tatatatata agttcatgat
     3001 tatggtaaaa tatatatata atcatgaact cagaatattt ttaagaaggg aaacaatagt
     3061 gagctataat gtacaaccgt taaggacaaa tgaagagata caagattttc agttttggct
     3121 aagaagaaca agtaatccag aaagagatag ttttttattt ttgtttggaa tcaataatgg
     3181 gttacgtatg tcagatatta taggattaaa agttggagac atacgaggta aaagtaagcc
     3241 tattattgtt gaacataaaa caggtaagcg aaaaccgatt tttatagata atttgagaga
     3301 agagatttta ttatatactg aaggtaaaga agaaaatgat tggttatttc caagtcggca
     3361 acaaggtcga catataacaa gggatcgtgt ctatcagata tatgcagata tagcagaaaa
     3421 attaggaaga gatgatatag gaacacatac attaagaaaa acttttgggt atcactacta
     3481 caagaaaact agagacattg ctaccttaat gtttattttt aatcatagct cgcaagcaat
     3541 tacaaaaagg tacattggta taactgaaga tgaaataggg gcatcgttac gtggttttaa
     3601 attgggaggc taattgagat attaggatat attttttgtg tataatgaaa acttagaagg
     3661 tgagattaat gaagataact gaattttcgg aagtatttaa aacgtttagt aacatttaca
     3721 atagaatacc agagatagaa caaaatgttg tagttgataa tgattattat atagagaatg
     3781 attttaataa accaagaaaa tatgacaacc tatacaattt tatacacaat agaacaacaa
     3841 taaataacaa cagtaacact cgagttttga ttaattcagc atattttcat attaaatatt
     3901 taaagaaata tgagtatgaa gaagttgtat tagattatgg gctaaataga cagtcatttt
     3961 taatttatgc aataaataac ggaaatttgc caagcaataa aaagaacata attattgaaa
     4021 catatgccta tctggagggg ggatctaaaa aactaattaa gtcatgtaat atagagtatc
     4081 aattatcagt aggagaaatt aaatgtgttt tcgaagaaga tttagaactt gacttaaaaa
     4141 aaatttttgc cagcattcat gattatatag gtttga
//
LOCUS       DACEPD010000141         4136 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.150, whole genome shotgun sequence.
ACCESSION   DACEPD010000141 DACEPD010000000
VERSION     DACEPD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.150"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..115
                     /locus_tag="IW797_002630"
     CDS             <1..115
                     /locus_tag="IW797_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1225376.1"
                     /translation="IKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(731..1771)
                     /locus_tag="IW797_002631"
     CDS             complement(731..1771)
                     /locus_tag="IW797_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ1225377.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2120..2446)
                     /locus_tag="IW797_002632"
     CDS             complement(2120..2446)
                     /locus_tag="IW797_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225378.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2433..3221)
                     /locus_tag="IW797_002633"
     CDS             complement(2433..3221)
                     /locus_tag="IW797_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1225379.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3589..3879)
                     /locus_tag="IW797_002634"
     CDS             complement(3589..3879)
                     /locus_tag="IW797_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ1225380.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
BASE COUNT         1286 a          691 c          591 g         1568 t
ORIGIN      
        1 gattaaaggc atggaagcca ttcgaggatt atataagaaa acccgaaaag aaggcactct
       61 cttcgggttt tcggtctgta ctgaaatcaa ggtattattg ggaatcccag cttaaatcat
      121 agataccgta agggatttta ttctttattt aaaactttgc aacagaaccg tccgttgcgg
      181 gtaataccgt aacactgaag ggcacgcatt aaggcgtcat cttaatgctt tcctgtagga
      241 cactgacgga gattaccagt ctcccgagcc ttacaggggc ctttcttttt tttgtatttt
      301 caattatcac aatttatcgt ccaaaagtgc agaacgaaaa tcaattcatc acaagcatat
      361 cagacaatta aatttaaaac aactaaaaat atacaaaaaa gtgtaattga atattagcag
      421 cactgcctga aaagaagtaa tatcaggctt ttcagcttgg ggaaataagt gaattacctc
      481 tcttcatgaa ttattataac tcaaaaaagc acatatgcaa acactagttc cctatttttt
      541 tgaatgaaaa ataactaaaa tatctaacga ttatgacttt aaaacctacg tttgtgattg
      601 ttttcataaa cttttaatct aaaaactccg gaataatagc tatttatttg taacatttgt
      661 aataaaatta aaatcataaa aaagttctaa tttaaaattg atcattttta tagtgatcgc
      721 tttttatcaa ctactctagc caattttccg tactaactgt tggtaattcg ttaaatgttt
      781 ctctttgttc gtttgcaagc tttgccgcaa tagctttttc gacaaaccaa ttttttacat
      841 aaacgaaaat tagattctgc aaattgttta ctttttcagt tttttgtttt tgataagctt
      901 tcagcaaagt catatataat cctttgtcag catcaattcc ataagattcc agttcttcat
      961 aaacaacaac tgtttgctct aattcttcag cttttttctt tgcattatga atagtttgga
     1021 cggttttttg agcgtcttcg taagtactag aaaatgtttt tatcaagttt aatacatttt
     1081 ctggtacaaa tgtatttata ctgtcctctt tcatgatgtc gacgaagtta tctagtaaaa
     1141 cttgattttg taactgatca gtttcagtgt ctattatgtg tctattagtg tctatagaat
     1201 tagaatagaa tagattgtct gcgattttcg caattacggt tgagtccttg atatgactgg
     1261 attttgccgt atttgcgatt ttcgcaatta cggtggtatc attgatttct cgcggttttg
     1321 ccgtatttgc gattttcgca attacggtgg tatcagtatt gtcttcctct tccatgattt
     1381 taaagacgtc ttgattcgtt acttctggct tgcctaagta caataaatta ggcatggatt
     1441 caatcttacc gtcacgttta tttactcttc ctctcttctg tttcagaaga cctgcttttt
     1501 ctaattcctt tttgatttta gatactttac cttcgcggca ttgtaacaat ttcattaatt
     1561 cttcaactgt gaatatgaaa aatatattat catcagaatc tacccagtta tttttgactg
     1621 aataatcaaa tcgatcttta agtagcatat aagcaatttt tgcatcattt gatagatcca
     1681 tgtatttttc gttagtaaaa aataccttag gcatttgata atatttttca cgataaattt
     1741 cttttgattt gtaataatta aaatcattca tagccaatcc tctttctgta ggatagcgtt
     1801 ttgttcaaaa ctatgttaca atgaattaaa caaatatatc tatccactat atatttttct
     1861 actgctgata agtcgccaaa ctttgttaaa gcagtagtct ttttttatct ttttttagtt
     1921 taccttaaaa aataaattaa agcaaaccgc tctatttcaa ggtttaagct catagtaact
     1981 atatcacgat attacaataa tattatatca cgatatcgta atattacaat atgaagataa
     2041 gcgacttatc agctttgata ctactgattt ttataaaaaa aatatcacga tattatgata
     2101 tcgtgatatt gaatgtaaat taaattttat tttgtttctt tgctagttta tatccttcaa
     2161 tcaagtattc ttcaggtatg gactttaata gcgcatcttc tataacgtct ggtttacttt
     2221 tgtctgattc agcaatagtc gtccaatatt tgagtgctga cattagaatt ggatttatac
     2281 gaatgctatc tgctgttctt acactacttc cttgatcttt tatgacttta cttatatttt
     2341 tcttggttaa ttcgatttct tctacttctt ttgatttaaa attcttttta tttttatttc
     2401 ctagtaatct acttactgca gcttgactgc gattatcttc tgccatcaaa atcaccaact
     2461 tcttttaaat attcttctgt aaatgcttta tagtcatctt cagctgttga gggtttatta
     2521 aaattgatta gatctgatcc agcagcttgc gcttcgtcaa cggttattgt cctacgaata
     2581 taagttttga aaatttttgc accaatgact ctagaaattt cggttgctgc agacgtcatt
     2641 tctttagaaa tgttataacg cttattatac ttagtaaata gaactcctcg tacttcaagc
     2701 ttgtcgttcg tatattcttt taccgtttca atagtttcac ttaactttat tagtcctttt
     2761 aatgagaaat acgcagcttc aactggaatg ataatttcat cagaaacagt aaatgcgttg
     2821 actgtaagca ttcctaaact tggtggacaa tcaattataa tataatcata gttgtccttg
     2881 attggttgaa gtactctttt aagcctgtgt tcacttccta cttgagtaaa ttccctctct
     2941 gcagaagaaa gggctatgtc ggctggcaaa acatcaattc ctttatagtc cagaatagca
     3001 tctgtaatgt cagcctcccc ttttaatact tcataaattg taggttggtt tgttccatct
     3061 atatcgaaac ctacattatc tgtagcattt ccctgaggat caatgtccac taaaagtaca
     3121 tttttaccgt aaacaaattt caaacatgct gctaaattaa cgctagtcgt agttttagaa
     3181 actccacctt tttgattggc tactgacaat attcttgcca ttaaaatatc ccccattcat
     3241 cttatattat aatataatta tatcataata ttgtgatatt gcaatatcgt atatgcatta
     3301 tattgtaata ttgtgtgatt attttgtttt aaagatatat atcggttcgg atataaagat
     3361 tattcatatg ttattcgaaa ttaaaataat cactttttat tactttaaaa ttttttatta
     3421 atgataagaa atatgttttg cgagcagcaa ctagaataat ttaaacatta ctaaactgtc
     3481 tatattgtta ctaataattc ccttcttata gtgcattttc ttagaaaaaa acaatattac
     3541 gatatcatga tattaagata tcgtaatatt atacaattgt gattgatatt atatatgttt
     3601 ttttattttt ttatcatgtt taatcttgaa ggaatcatat tctttttcta aagacaggag
     3661 cttttcataa acatcagaca tgtttaaact agaggccagg cttaaatcat aaacgaatga
     3721 agcaggtaat gattttattc tacgttgtcg ggttatccag cttgcaaccg ttccttgtgg
     3781 gtaggcatat aactcacaaa attgttctac agtgattcct aaattcttga taatgtaaac
     3841 attgatcggg tgcgagtaaa caaatgtatt gatagacatg gtgcgtaagc ctactttctt
     3901 ttattattgc gtttgcaata atagtttaac acacgcatta ttctagtatc tactcctatt
     3961 tgtaaaaaca aaaaaggatt catagagata actctataga atccgtaaaa taccttatat
     4021 atataacggg aagatataaa acctaaaatt atgataacct gctgcagctt ctacatagaa
     4081 agagaagcgc tcaacaccaa cctcatttat caaacgttgg ccataacccc aaatct
//
LOCUS       DACEPD010000142         4086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.151, whole genome shotgun sequence.
ACCESSION   DACEPD010000142 DACEPD010000000
VERSION     DACEPD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.151"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1287
                     /locus_tag="IW797_002635"
     CDS             <1..1287
                     /locus_tag="IW797_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAQ1225381.1"
                     /translation="TIYDSVNARYDGRVGKDTLESWYKSVVEAEKNNMTFLSFSDNPH
                     SKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDFSNFSINENEVLLE
                     KLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLGKEAELDQFSEIQS
                     DYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIYEFTQENGLVEKET
                     ERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKNEIETCVNEIRKLN
                     WDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKETDIYRELKRDVEA
                     LKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKIQTIINEMSDEIDS
                     YRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            1290..2186
                     /locus_tag="IW797_002636"
     CDS             1290..2186
                     /locus_tag="IW797_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ1225382.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            2236..2625
                     /locus_tag="IW797_002637"
     CDS             2236..2625
                     /locus_tag="IW797_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1225383.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            2612..>4086
                     /locus_tag="IW797_002638"
     CDS             2612..>4086
                     /locus_tag="IW797_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1225384.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPLSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT         1453 a          601 c          828 g         1204 t
ORIGIN      
        1 actatctatg atagtgtgaa tgccagatat gatggtagag ttggaaaaga tactttggaa
       61 tcttggtata aatcagttgt tgaggcagaa aaaaataata tgacatttct tagcttttct
      121 gataacccac actcgaaagg aaattcaaaa catggacttt ggggaatagg cacaagatat
      181 tcctatataa aaatgaagga cgaacctgac ttgggatcac ttcgcgatgc tttaatgcta
      241 ggcgaaactc gagttcattc tgatttttct aatttctcca ttaatgaaaa tgaagtttta
      301 ttggaaaaat taagcttttc tgggaccact cttagcacaa aagaagttgt agtcgaattc
      361 agcgataatt taacttcaat tattggagga agaggaacag gcaaatcttg tattacaaga
      421 ttcttggtct atgtccttgg aaaggaagca gaacttgatc aattttctga aatacaaagt
      481 gattatcaga actttgcaca aattgaacat aatggctctg gaatattttt gaaggataca
      541 attgtgaaac tgaatatttt ctataagggg acaaaatatc aaataataag aacacaggat
      601 agacattcta tttatgaatt tacacaagag aatggtctgg ttgaaaaaga aacagaacgg
      661 ttgagaatga tttccgataa ggtgagtata tactctcaaa aacaaattta tgaaatttca
      721 aaaaatcaaa ctagtatact agaattacta gatggatata actctgattt gatttcagag
      781 tataaaaatg agattgaaac atgtgtaaac gaaataagaa aattaaattg ggatattgtt
      841 tctgtgaaaa aagaaataca agataaggcg aaggtagagc ttgaaattga agatttaaaa
      901 aaacaagtag aaaagctatc tcataagagt tataaagatg tttatgatga gtattcaaaa
      961 gagacagata tttataggga actgaaacgt gatgtggagg cacttaagga aattcccaaa
     1021 aatctaactg atagtgttga caaaattgac tttggcaatg gtataaaagt tacagacgaa
     1081 attgatgaac accgcggaga attgattaag aatttagtga ccaatcgggc taagattcaa
     1141 acaataatta atgaaatgtc agatgaaatt gatagttata gagcagagct gaataaatca
     1201 gattggaaag ttaactataa tgaggtttca aagaggtatc aaagaggtat caaaaactta
     1261 gtgaagagtt atctcaagat gaattaaaaa tgttacaaaa ccttgattct ttatatcaat
     1321 ctttatcaaa aactgaagag cgattggaag aaattattca acaagaaata aagcttaaag
     1381 attaccaagt tgaggttgac tcagcaatgt caagaatgaa taaatcatat caaaatttct
     1441 attctgagag aagtagttcc tcgcgcgaaa ttttttctga tattacagga atagaggcta
     1501 ctattcaacc aaatagggat ttcaataatt atgttaaaga gctcagagct tgtattaata
     1561 aggaggacac ttttgacagt gagtttggat ctcttattga taagctgaca aaaaattcca
     1621 ttactaatca agatatatat aatgacatac ttgatataag agttagtggg gaaagtagta
     1681 ttttttcaga taaaaaatta atcaattcta taagaaaatt gaatgatgca caagttcttg
     1741 aattacaaac ccttaagcca aatgatttag tagttatccg acttgaacta aatggaaaaa
     1801 aagttagttt atcgaatgct agtgctggtc aaaagacatc tgcaattctt tctatgattt
     1861 tggcgtatgg aaattcacca ctcatattgg atcagcctga agatgatttg gatagtcaat
     1921 taattaataa cttaatcgta agaagtatca ttacaaaaaa ggaaaacaga caaattttaa
     1981 ttgtaactca taatgcaaat atcccagtta atggtgattc agaatggttg attagcatgg
     2041 gagattcaaa agaaatatcg gctgatcagt gcgggtcggt agatgaaccc aatatcaagc
     2101 caagaatatg tactgtgatg gaaggtggcg aggatgcttt tacaaataga gcgaaacgct
     2161 atggttttaa acaaattgta gaataacact agagggtgag acatattatt atgtccgccc
     2221 tttttaggag gaaaaatgaa aaaaataaaa agctatacca gtatctgggc agttgaaaaa
     2281 gtcatttacg caatcaatga ttttcagttg ccttttcctg tgacttttaa ccaaatggct
     2341 tggttcgttt tatctttact gtttgtcatc gtatttgccc atgtgcctcc actatctatg
     2401 atagaggagg tctttttaaa atacttaggc ataccggtgg ctgtcacttg gtttatgtcg
     2461 caaaagacgt ttgatggcaa gaaaccactg ggatttttac gatcctttat cagctatcac
     2521 ttgcgattta aggtgacttt tgcaggaaag aaagtcaaag aacagaagaa acgatgggat
     2581 gaacccagta ctcttgtaag gagtgtgaac tatgtatccg attaagtata ttgaaaataa
     2641 tctcgtcttt aaccaagaag gcgagtgttt tgcctactat gaattagtgc cttacaatta
     2701 ctcctttttg tcacccgaac agaaatttca agtgcatgat aactttcgcc aactaattgc
     2761 gcaaaatcgt gaaggcaaga tccatgcgtt acaaattgcc acagaaagta gtattcgcgc
     2821 cacacaagaa cgatcaaaaa aagaaattac cggtagactc aaagaagtag cgaaacaacg
     2881 aattgatata cagacagatg cattagtatc catgattggc gatagtcaga ttgattatcg
     2941 cttttttatt ggctttaaac taattgcgac agatgaagaa gtcaatctaa agagtctcaa
     3001 gaaatctttc ttttctgggc ttcaagaatt tgtctatgga gtgaatcatc atttgatggg
     3061 cgattttgtt tccttatcca atgaagaaat tcgctgctat tctaagcttg aaaaattaat
     3121 ggaaagcaaa ttggctcgaa gatttaaagt caggcgagtc acaccgagtg acctgacgta
     3181 tttgattgaa catatttatg gcgaaaaagg gataccgttt gaagaatacg aatttcagct
     3241 accaaagaaa aagctaaaat cagaaacact ggtgaaacgt tatgacttat tacgtccaaa
     3301 tcgttgttta attgaagaaa aaccaaggtg cttacggatg gaacatgaga atcatgagtc
     3361 gtacgtagcg tatttaacaa ttaatacaat tgtgggggag atggaatttc cttcctcaga
     3421 acttttctat taccagcaac aacaatttac attcccaatc gacacaagta tgaatgtaga
     3481 gattgtgacg aataaaaagg ctttagctac cgttcgtaat aagaaaaaag aactgaaaga
     3541 tttagacaat catgcttacc aatctgacaa tgaaacgaat tccaatgtct tagatgcttt
     3601 ggattccgtg gatgaattgg aaaccacctt ggatcagtca aaagaatcta tgtacaaact
     3661 gagttatgtg gttcgagtga gtgccgaatc agtggatgaa ctaaagcgac gctgtgatga
     3721 agtgctcgat ttttatgatg ataccaatgt aaaactagtc cggccctttg gcgatatgat
     3781 ggggctacat gaagaattct tacccttaag taaacgatat atgaacgact atattcagta
     3841 tgttacgtct gattttttgg cggggcttgg ttttggggct actcagatgc taggagaatt
     3901 agaagggatt tactttggct acaatgtgga tacaggtcgt aatgtttacc tgaaaccggc
     3961 attggcttca caaggagtca agggttcagt aaccaatgcg ctggccgccg ctttcctggg
     4021 ttctcttggt ggaggtaaat cctttagtaa caatctctta gtctactatg ctgttttgtt
     4081 cggtgg
//
LOCUS       DACEPD010000143         4065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.152, whole genome shotgun sequence.
ACCESSION   DACEPD010000143 DACEPD010000000
VERSION     DACEPD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.152"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(77..1051)
                     /locus_tag="IW797_002639"
     CDS             complement(77..1051)
                     /locus_tag="IW797_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1225385.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVPFMDTPLKIQWSIDRITIVG
                     NLKENIYYHTTHDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLELQ
                     LRRGKATDWHAMVQESLDSFASPHYLPADTSVADKMMITALTTEHDYWGQINRKTKYK
                     YRNLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
     gene            complement(1500..2915)
                     /locus_tag="IW797_002640"
     CDS             complement(1500..2915)
                     /locus_tag="IW797_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ1225386.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSAEMRYISKQREK
                     NEREKALVASEKAKIEEEKKSIIDLEKELAVVFQKDPVPLTSFEKLPDGSYRLSSADF
                     VDLYQKASNATNSKKYADMANESLGNTLDKNIALFDKNMALEQENSFLKNQQKVDLSA
                     VVAENKKLKEENRSLRQRLTQLKNAFQKSITRLSIRFGLVEKDMGISEELKILDQEKT
                     LASQQNAPERNIGRSENEMEW"
     gene            complement(3137..3499)
                     /locus_tag="IW797_002641"
     CDS             complement(3137..3499)
                     /locus_tag="IW797_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225387.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3503..3811)
                     /locus_tag="IW797_002642"
     CDS             complement(3503..3811)
                     /locus_tag="IW797_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225388.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLKLPAEDNYSEMLERMMDVL
                     NSTINAGLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3912..4052)
                     /locus_tag="IW797_002643"
     CDS             complement(3912..4052)
                     /locus_tag="IW797_002643"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225389.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYIYAVAESSGEA
                     ND"
BASE COUNT         1141 a          899 c          587 g         1438 t
ORIGIN      
        1 aaaaaaacaa ccacaacgta atcacctgtt aaatttacca accaattttt cttaccaaaa
       61 tacttttgtt tttctgtcat ttttcttcct cctctgttac gtctagccct agaagccatg
      121 tgtctaattc tttttttaaa tcgtctgccg attctgcaaa ggtttctcgt aaatgattcg
      181 tcaattcatc attttgactt tcttgcttca gcaaatttcg atatttatat tttgtctttc
      241 gattaatttg cccccaataa tcatgttctg ttgttaaagc agtaatcatc attttgtcag
      301 ctacacttgt atcagcaggc aagtaatgag gactagcaaa actatccaag ctttcttgaa
      361 ccattgcatg ccaatcagtc gctttccctc gtcttaattg taattctaac cgccaccaac
      421 tcacgatttc ttttggcaca atttttcttt tcttctcttg ttccagtttt ttattataca
      481 tccgaatttg acgttcagaa gagcgagatc cccaatatgc cgtctctaat tttccattcc
      541 gtccataaat aggcttaaaa gaaactggat caactacccg atattgtgta ataaactcat
      601 caggtacatc gataatgtcg caagcaatat ctgcacgtga aaagtgaggt tcgataaaca
      661 aatcatgaat aaagtttttc atggaactag ctaagaattg actaatctta ttcggattga
      721 agtctatacg ccctttcccc tcttgccatt ttaaaatttc aatataagca atattctctt
      781 catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa ccgttacctt
      841 catttaggcg cataagctgt tcaaaatcta aaatcaacac atcatgtgta gtgtgataat
      901 agatattctc tttaagattt ccaacaatgg taattcggtc gattgaccac tgaattttta
      961 ggggggtgtc cataaaaggg acacttaacc gcctgttaga gggggctgtt aatttttccc
     1021 actcatttat gatcgtgcct tttttactca aatttagttc ctcctcaaag aaaaaagaga
     1081 ttggtggaaa gaattttcca gagttcacgt aactctaaaa aattcataac cagcaatctc
     1141 tagtcttgaa gaaacctgaa attattcgta taatgaattt gagttacgga gctagtgtga
     1201 ttagtttccg taaaggtcga gaacgtgtcc agcgttgctc gactttattt ttttgctctt
     1261 ttttcagttt atttatttga taactcaata atagcacttc tctttagcta tgcaaatatc
     1321 caaaaacata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta acgctaagtt
     1381 ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt agtggtttag
     1441 ccgtcaactc cttatcctcg gcacagaccg cctccggtac cgttgactgc accgtataac
     1501 taccactcca tctcattctc agaacgccct atattacgtt ctggggcatt ctgctgacta
     1561 gccaacgttt tctcttgatc taatattttt agctcttcag atattcccat atctttttct
     1621 acaagcccaa aacgaattga taaacgagtg attgattttt gaaatgcgtt tttcagttga
     1681 gttaaccgct gacgcaaaga acgattttct tccttcagct ttttgttttc agcaacaacc
     1741 gctgacaaat caaccttttg ttgatttttc aaaaaagaat tttcttgttc taacgccatg
     1801 tttttatcaa ataaagcgat atttttatct aatgtatttc caagcgattc attcgccata
     1861 tcagcatatt ttttgctgtt cgtagcatta gacgcttttt gatacaaatc tacaaaatca
     1921 gcactagata agcgatacga gccatccggt aatttttcaa aactcgttaa aggaacaggg
     1981 tctttttgaa aaacaacagc cagctctttt tccaaatcga taatcgattt tttctcttct
     2041 tcaattttcg ccttctcaga agccaccaga gccttctcac gctcgttttt ttctctttgc
     2101 ttagaaatat acctcatttc cgcagaaacg ctctcacgct cttttacagc cgtttggcgc
     2161 aattcagaaa tttcactcat gatttctttg tattcgtgaa tatctttaat tttgttggta
     2221 ccgacaatat cccttttcca accgtaatca ttcattaatc gttctataga gtcaacttct
     2281 tgatttctaa aattattgaa aagcactcga ccattatctt tatcagcgtg tatcccttgt
     2341 tgaaccagtg ctttattaaa gctcggctgc ttttgaacac cccgtttata accttcagca
     2401 accggcacaa tattcaagtg caggtgcgga cttatttcgt cattatgaat aacagcatta
     2461 taaacagcca aattagggtt tctctcttga aactccttgt aatactgaac caaaatttta
     2521 ttggcaatca ctcgattttg ttcagcttgt tgtttcgata tgccagtaga agaaccatct
     2581 gctcgatttg tctggaaatc atctaaaatg cctatttgca caataaattc tcgttgaggt
     2641 tccaactttt tatccttgcg cactttcgct aaatagtcct cgatttttct atcggctctt
     2701 ttttgtttcg cattatattt ttctaccact tcgccaaaca attcctcata tttatctgct
     2761 agattcgtct gctctaaata aatattttca tccgatttcg tgaagtcaat gtgcttatgc
     2821 caatcattgg ttttctggcg atcagttaac tcacgattat tatgagaaat acttgtattc
     2881 ttcgttcctt ttttaaatga aatactaaac cccaagttaa aaccctcctt cgcgctgtcg
     2941 ctaataaaaa gagtgtaaac ctttctgtta ctctttgtca aagagtaaca ttttcctaat
     3001 tgtgacatgg ctgtcacaat tccaaatgct ctccgagggt attcgctgtc acaagtgaca
     3061 acgaaaaaag gagccgaaag cgtgtcacgc tctcaactcc ttttacgctt tattttgctt
     3121 cacgcaaaat aatcgcttat cgtaaattca cgtcattaaa tattgacgta aatttactct
     3181 cactcatctg acttttctta ttcggtatca ctccgaataa gccgtcagat tcatcgggat
     3241 aatctcgttc gcttggcgct gatttttcat cagcaagcca ctcattatcc actgattttt
     3301 cgtgttgctg atcgtcagaa atcgacgata gccaccgctc aaaatcagca aaagtttcaa
     3361 acccaaaacg cttccgaagt gccttaccca tttcagcatt ttctttctca atcaagcgtt
     3421 ttttctgttg gcgaagtttc gcttgttctt tttttaattt ttcttcttgc gctttaatat
     3481 ctgctaaagt cattcccata aactacatct ccttttcttt ggaattaata acggaaattt
     3541 ggtttaactg cataacatga ctcattaatt tcaaccaaaa ttctttttct gattcatcag
     3601 ttgtttgcgc taaatgctta tgaacaaatt cattagtcaa aactgaagat tttgctaaac
     3661 cggcattgat tgtggaattt aaaacatcca tcattcgctc aagcatttca ctgtaattat
     3721 cttcagccgg caatttcaaa taatcagcta accgctcata catcaattca attctatcaa
     3781 ctgtctcttc tcttaaatca attttatcca tgttacacct catttccaaa actgccacca
     3841 tttccgattc tttaattttc gatacaccta attcatcagc aagatttttt atggtcatct
     3901 tttctgacaa atcagtcatt agcttctcca ctactttcag ctaccgcata aatatattta
     3961 acggcgtgct tagggataat aatcggagag tcaattttat tatttttcaa aatcaattca
     4021 tattgcgtta catcaagtaa cttaccggac atttttttgc tatcc
//
LOCUS       DACEPD010000144         4038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.153, whole genome shotgun sequence.
ACCESSION   DACEPD010000144 DACEPD010000000
VERSION     DACEPD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.153"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            179..775
                     /locus_tag="IW797_002644"
     CDS             179..775
                     /locus_tag="IW797_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ1225390.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="IW797_002645"
     CDS             899..2698
                     /locus_tag="IW797_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ1225391.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="IW797_002646"
     CDS             complement(2976..3188)
                     /locus_tag="IW797_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225392.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT         1186 a          689 c          712 g         1451 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaat
//
LOCUS       DACEPD010000145         3961 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.154, whole genome shotgun sequence.
ACCESSION   DACEPD010000145 DACEPD010000000
VERSION     DACEPD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.154"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(609..1166)
                     /locus_tag="IW797_002647"
     CDS             complement(609..1166)
                     /locus_tag="IW797_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225393.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1345..1956)
                     /locus_tag="IW797_002648"
     CDS             complement(1345..1956)
                     /locus_tag="IW797_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225394.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2069..2326)
                     /locus_tag="IW797_002649"
     CDS             complement(2069..2326)
                     /locus_tag="IW797_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225395.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2923..3201)
                     /locus_tag="IW797_002650"
     CDS             complement(2923..3201)
                     /locus_tag="IW797_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225396.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3235..3486)
                     /locus_tag="IW797_002651"
     CDS             complement(3235..3486)
                     /locus_tag="IW797_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225397.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1201 a          810 c          526 g         1424 t
ORIGIN      
        1 tatttcttgg agttaaacgc ttctgtccca gcctccttat tccgaataaa tggtgtccaa
       61 aagcttgcat ctgccagatt atctcatttc ttatgacaag ctaagcaaat tatctccacc
      121 aaataattta ttcatatttt tgacttgcgc atactaacta attcttaaat tttcatacaa
      181 aatttctcaa tttcccttaa cgagtaatat ttatgtacgg tgattctcac tagtatccca
      241 atcacttttt aagttatccc gatttcaatt ctacttcaaa aatagttgct tttatgtatg
      301 ctgttagttg attagatggt catttgttga gctaggtcgt ttattttaaa caagttgttt
      361 ctttagctta tacctatctc gtctaccaaa cgcaagatgg caaacacaaa tgcatacgca
      421 tctgtatttg ccactttgat tcgtataaag ctaacaacta ttaactcctc tcagctaata
      481 gttgctctct tttttattca gattcatttt tgctgatcac agcctagtat ttcaatggtt
      541 ttgaacacta aaccaaattg ttccattaca acattcactg gctgatgaaa tggattagta
      601 gatttcgatt attcaaagcg attttctatt ggtggatcgt gttcccaact aataagacgg
      661 attccaaacc tatcgtacag atcttctaaa ccacttataa attttgcacc tatttcaggt
      721 gttttattgc gtggatattc tattgttacg ccattaaatg gataccaatt aggattaggc
      781 tctctttttt tctgtacatc tcccatagaa tagttagcac tttctgggac ataatatatt
      841 cttcgaccat cctctaattc atctaataag atatttcgtg atcgtctatc acttgctaaa
      901 tgtagacgcc acgtgcgata ccatgccatg ctacctgcat ctgcagaaaa tccatcaaat
      961 aatgcaacaa ttgtatcttc tatcacaatc ctttccccat ctacttcata ttctaatcta
     1021 aaatcaaact cccctcgggt aatttctggt cttggtggct taggacccaa tattattcta
     1081 cttaaaaccg cattcgttcc cagatgaaac acagctataa caacaataat gattaatatt
     1141 ttgtttctat ttgataaatt tctcattatt cgcacatcac ctctacattc tcacatatta
     1201 gttcgtaata ctcctttaat atcaaaatat ctactggtcg taataatatt cgctatgaat
     1261 tcctactact cgtataagaa aggccaaatc atcagctttt acttgctgat gatttggctt
     1321 gtttctattt atcgtaattg ttttctaatc accaaaattg ttaacaatcg gttcagatat
     1381 ttcccagttg attaaggtaa ttccgtattg tgtcaaaact tcttgcgctt ctccgaaatt
     1441 aagtacagtt cttatgtatc cactttctcc taatataaag tctacctctc ttttaaaacc
     1501 ttcctcgaga tttctaaggg ctatatgtgg ttttaatgac attgattgta cattttcccc
     1561 catataatat tgcgcacttc ctcgactaaa atagattcct agatcgtccc caaaaggtag
     1621 atcttctcta ccactagcta atctacttct ccatcttcgt tctcttttca tagaacctgc
     1681 gctgaactca cacccgttaa actcagctat gacagtatct tcaattatgt taatctgtcc
     1741 atctagttcg tattcaatcc taaatggaaa ttctccatag gtgatttccg gttcgggtat
     1801 tttaggtgag attaatgcaa agaaaggaaa tattaagctt ggcaacatca aaaaaactcc
     1861 tcctatgatc acaattcccg atgtaatcca aaatataatt tgtacaattc tcattgatcc
     1921 attgtattcc attttattcc ccctattact attcatatcc gcctatctat atcgatcttc
     1981 caatgcagag ataattgctt ccataccttg attttttact gagttaccta tgcgagatat
     2041 cattctccac taaggtgcat catattgtct atctaacatg attaattctc caataggtgt
     2101 atactcctcc aaaaacaaca tttaatgcat ctccctcgac aacataagct accatgtttg
     2161 ctgtatatgt gtttgagtct aattcataag cagataaatt tactgtcgct ggattaaata
     2221 taatcgcatt cgtatttata gctactgaat ttgctgcttc agcaccacct tttgaatgtc
     2281 cgatcattgt gatttcaata gtaggattta cgtggacagc cctcaccgtt taccctgacc
     2341 attatagaaa tcgcttactt ttttgaacta cgcaacaaag gatacaatgc tcctaacttc
     2401 actgtctcca tttttgtcca ctaaggaata taaaaaatag gcattaattc atttaattcg
     2461 gtcacaacac gttacctttt aaatcattat actgtatctc atacattatt atcaataaac
     2521 tattataaaa atgcaaaaaa tgctttatca atctattttg taattggtca taatatcatt
     2581 gaaatcatac cctgtatcga tttttcctat gaatctcgtc tatttgtgat tgacgaaaaa
     2641 gaggcaagac agacactgga atggtaattt tacccactat tttgctccaa ctatttgtaa
     2701 tcgtacccac ttttgaaata ccgagatgat cattttaatt gatatgctga ttcccaagca
     2761 aaaccccaag aaattaaatg ctctatcgca tctagcttct tgggttttgc ttatataaat
     2821 tccaacattt aaatttttta tgctgttcgg tctcagaaat gccgtttttg cttatatcac
     2881 aatatcaaga tgtcaaatta ttcgtgttgc cgatgacttt gattaatttt tttcaaattg
     2941 tttattaaga ctaatgctgt ctttttgcag ataccaatgt tcgaactttt taacattatc
     3001 atatgatcct gcaagtttta acttcttacc attagatgtt atcaaagtta taattaacct
     3061 tgcctttttc ccattaggat cttttatttt atgaattttc tgcaggtaat ctctcctaat
     3121 ggcagctatt tttacctcat cagtgctgat aaaccattga tcatccacat acatactgcc
     3181 tgttatgttg tgtttttcca tttcttccga gaacgaaaag tttaaggctc gttcttactt
     3241 ccaaagttga atgagtccat ggattggact tagtgtgaga attgagaaca acaataagaa
     3301 tccatatcct cccgcaaaca tccctaaggc tatactgtct ctccatccat aagtaggatt
     3361 agaagagaaa agagtcaccg tttgtaataa tatgaaaagg aataatccag cacaaacacc
     3421 aaagataaca actccaaaca ttgaattttt atacaatgac catttattta taggcttttc
     3481 tttcatttta cttcccccta ctgttctgtt cacacggaat gaattcaaag ttcataagcc
     3541 ctcctctttg aacatatgaa tacgacttgc taactatact ttaaaaaagc ttgttttaag
     3601 cggttatagg cgagttgtaa tctctattca tctcattagc aaataacttg ccgaaaagtc
     3661 tactgaaatt aaagcagata gtggaagaat tatttagcca ggatagacac tatttgtgaa
     3721 acacatgtct ttacttttga aattctatga tgaaaagcct tggaatacag tagctcttaa
     3781 ttggtaatac tttttccatt ataaataacc tcttttcatt ttatatttat cccctctaaa
     3841 aataatatct aatctaatat tttaaactta tatttccctc gctgtgctaa tagagcatta
     3901 ctaaataaat agaaactaga aaaaagcatt acgcctgcca gaataaacca attatttgta
     3961 t
//
LOCUS       DACEPD010000146         3886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.155, whole genome shotgun sequence.
ACCESSION   DACEPD010000146 DACEPD010000000
VERSION     DACEPD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.155"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            394..1083
                     /locus_tag="IW797_002652"
     CDS             394..1083
                     /locus_tag="IW797_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ1225398.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1070..2278
                     /locus_tag="IW797_002653"
     CDS             1070..2278
                     /locus_tag="IW797_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ1225399.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2572..3843
                     /gene="serS"
                     /locus_tag="IW797_002654"
     CDS             2572..3843
                     /gene="serS"
                     /locus_tag="IW797_002654"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ1225400.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1278 a          670 c          782 g         1156 t
ORIGIN      
        1 caagtggttc ttttttaacg actcagttat cttatcatgt catttccaac atgtcaacaa
       61 cttatttttt attatttaat aagttattgg tgttcttttg aacaaataat aatatatcaa
      121 catcacatgt gaaagtcaat aagaaaatgc ttatttatca acatttctag cttacatgat
      181 cattggttag ttctggtcct ttttcgttcg tctttataac tccgagagac gtaaaatcct
      241 tttgttttca gtttaatgtt catgttcttg tcttattcac accaactaga tgtctcacct
      301 tctaaaattt tccttaacaa agtgctgctt ttatctattt ctgctttttt ttgctagaat
      361 aatagtaaca atatgactct ggaggattta tttatgaaaa ttttgattgc tgacgatgat
      421 aaagaaatcg ttgaattatt aagtatctat gttcacaatg aggggtatga agcagtaaag
      481 gcttatgatg ggaaagaagc attatctaaa atcagaacaa tccctgatat tgaattactt
      541 attttagata tcatgatgcc tgagatggat gggatgcaag tagtgaaaga gctacgaaaa
      601 gaatcccaaa taccaatcat tatgctgaca gcaaaaacta ctgatatgga taagatcaaa
      661 ggactggtcg ctggtgcaga tgattatgtg accaagccat ttaatccgtt agaagtaatg
      721 gctagagtaa agtctttatt acggcgtaca aaaatgcaga ttgccccaga tcagcctgat
      781 gtattagaag taaatgcatt aatcatcaat aaagactctc atgaagttaa aacaatcgat
      841 gggaaagaaa tccagttaac agctttggaa ttcggtatcc tctacttgct tgctagtcat
      901 cctaatcgag tatttagcgc ggatgaaatt tttgagcgtg tttggaaaca agaaagtatt
      961 gtctctgcta aaactgtcat ggttcacgtt agtcatttaa gagataagat tgaagaagca
     1021 actggtggtg aaaaagtcat tcaaaccgtt tggggagtgg gctataaaat tgacgcaaga
     1081 tagtaaacga gcaactgaaa aacgccgtcg gatcaatttg acttcaaaag aaatcagcga
     1141 attgttagcg gaaggaatca tcacgattat ccttttgctt ctattaaatg tttctattct
     1201 ggttgttatc agttcggtga tcaacagttc accttctttg acgaatgcga tttgggattc
     1261 aaagaatatt tttgcagaac gattgaacac tgatttgttc tggaacggcc ggaattttat
     1321 tattcccttt ttctttctat tagatattgg tgttttgtat tggcgcttga ttcgaagata
     1381 tcgccaaatg caattgcggc atatcatcag cgaacttcac tacatcgcaa acggaaatta
     1441 tgatcatcgt atcccttttg aactgagtgg tgacttaagc cgagtggtaa caagtatcaa
     1501 tggattggtg gatagtaccg tagcagcgat tgaagacgaa cgtaaaatcg aaaaatcaaa
     1561 agatgaattg atcacgaatg tcagtcatga tatccgtacc ccactcacgt ccatcattgg
     1621 atacctagga ctgatagaag atggccagta tcactcagaa gaagatcttt taaagtatac
     1681 gcatactgct tatatcaaag caaagcagat gaaatcttta gtggacgact tatttgaata
     1741 cactaaggta cgccagcctg ctgtcccggt gaatttttct gcctttgaca tgatccagtt
     1801 gatcgaacag ttagcagctg attttgagtt ggaagcttct aaaaaaaata tccaaatact
     1861 tgttcaatca aaagtagact cccttatcat ggatggcgat accgaaaaac ttgtacgtgt
     1921 cttcaataat cttttgacga atgcgttgaa atacggaaaa ggagcaacga agatcgtaat
     1981 cgaagtagaa cgtatcggtt cagaagttgt tgcaacagtc aaaaataacg gagcaatgat
     2041 cccacaacag gcaatcgata atctattcga ccgtttttat cgtgtagaag aatctcgttc
     2101 acaagcaact ggaggaactg gacttggttt agcaatcgct caaagcattg tggctttgca
     2161 cggcggatac atttatgcaa aatcagataa gcaatggact tctttcatca tgcaccttcc
     2221 aatcaagaaa aatgaaaaat tacctattga tacacaggaa gttattgacg aaagctgaaa
     2281 tattcgctat tcttattaca attattagaa taaatgaaaa acacagaacc cagtacactt
     2341 tattcttttt cagagagctg atggcaggtg taaatcagca aaggataatt tggaagttcc
     2401 agttcgttta cgtgcccttt cgaaataaga aaggtcggtt caacagccgt tatgtgttca
     2461 acaagtgcag ctgacgctga acttgggtgg taccgcgaat ttcagactga ttcgtcccaa
     2521 gaataatggg atggatcagt ctttttttat tttcaaaaag gaggaaatca tatgttagat
     2581 gtaaaaatga tgcgacaaaa tattgaagaa gtacaagaaa aattgatgac tcggggcgta
     2641 aaaaaagaaa cattagctga attcatggaa ctagatgaaa atcgtcgtaa gctactagtc
     2701 aaaagtgaag aattaaaaaa atatcgaaat gatgtttctg ctgagatcgc ccaattgaag
     2761 cgtgcaaaag aagatgcttc tgctaaaatc gctgaaatga aagaagtcgg tggcaacatc
     2821 aaagcattgg atactgaaat tgctgaagtg gatgaaaaat tgaaacaaat tgcaactact
     2881 ttgccaaacc ttccccatga ttctgtgcca gttggaacag atgaagagga caatgtagaa
     2941 atccgtcgtt ggagcgaacc acgtacattt gcttttgaac caaaaccaca ctgggaaatt
     3001 gcagaaaatc ttgatatttt agattttgaa cgtggcgcaa aagtagccgg tagccgtttt
     3061 gtctattaca aaggattagg tgctcgatta gaacgtgctg tctacaactt catgcttgat
     3121 cagcatattt acgaacacgg ctatactgaa atgatcactc cttatatcgt caacagtaaa
     3181 gcgatgtttg gtacgggaca atttccaaaa ttcaaagaag atgttttcca attggagaat
     3241 actgatctaa cattaatccc aacagctgaa gtaccattaa ctaactatta taatgatgaa
     3301 attttagatg gtgcagattt acctatttat ttcactgcac taagccctgc ttttcgttct
     3361 gaagccggaa gtgctggtcg ggatacacga ggactgattc gtcttcacca attcaataaa
     3421 gtagaaatgg tcaaattttc ggatgtagag cattcttacg aagaattgga aaaaatgaca
     3481 gctgatgcag aagatatctt gaaaaaattg catttgcctt atcgtgtgat ggccttatca
     3541 actggagaca tgggcttttc tgcagctaaa acttatgact tagaagtatg gatacctgca
     3601 caagatactt atcgtgaaat cagttcttgt tctaactgtg aagatttcca agcacgccgc
     3661 gcaatgatcc gttatcgcga taatgatgga aaaatccact atactcatac tctgaatggt
     3721 tcaggacttg cagttggccg tacagtagct gccattttgg aaaactacca aaatgaagat
     3781 ggttctgtca cagttccaga agcgctcgtt ccttatatgg ggaatctgaa agttattaaa
     3841 taaagaggct gtgaaaaagc cgcttagttt cgagtattaa gaaaaa
//
LOCUS       DACEPD010000147         3812 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.156, whole genome shotgun sequence.
ACCESSION   DACEPD010000147 DACEPD010000000
VERSION     DACEPD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.156"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            98..2197
                     /locus_tag="IW797_002655"
     CDS             98..2197
                     /locus_tag="IW797_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ1225401.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2393..3256
                     /locus_tag="IW797_002656"
     CDS             2393..3256
                     /locus_tag="IW797_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ1225402.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3361..>3812
                     /locus_tag="IW797_002657"
     CDS             3361..>3812
                     /locus_tag="IW797_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ1225403.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEK"
BASE COUNT         1523 a          636 c          772 g          881 t
ORIGIN      
        1 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
       61 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      121 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      181 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      241 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      301 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      361 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      421 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      481 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      541 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      601 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      661 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      721 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      781 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      841 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      901 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
      961 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1021 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1081 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1141 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1201 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1261 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1321 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1381 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1441 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1501 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1561 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1621 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1681 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1741 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1801 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1861 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1921 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     1981 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2041 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2101 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2161 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2221 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2281 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2341 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2401 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2461 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2521 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2581 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2641 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2701 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2761 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2821 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2881 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     2941 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3001 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3061 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3121 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3181 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3241 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3301 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3361 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3421 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3481 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3541 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3601 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3661 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3721 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3781 catggaatct gtcaggaatg ctatgaaaaa ga
//
LOCUS       DACEPD010000148         3740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.157, whole genome shotgun sequence.
ACCESSION   DACEPD010000148 DACEPD010000000
VERSION     DACEPD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.157"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            598..1926
                     /locus_tag="IW797_002658"
     CDS             598..1926
                     /locus_tag="IW797_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ1225404.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2128..2961
                     /locus_tag="IW797_002659"
     CDS             2128..2961
                     /locus_tag="IW797_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ1225405.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2977..3714
                     /locus_tag="IW797_002660"
     CDS             2977..3714
                     /locus_tag="IW797_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ1225406.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
BASE COUNT         1252 a          585 c          781 g         1122 t
ORIGIN      
        1 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
       61 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
      121 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
      181 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
      241 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
      301 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
      361 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
      421 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
      481 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
      541 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
      601 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
      661 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
      721 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
      781 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
      841 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
      901 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
      961 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     1021 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     1081 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     1141 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     1201 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     1261 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     1321 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     1381 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     1441 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     1501 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     1561 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     1621 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     1681 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     1741 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     1801 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     1861 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     1921 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     1981 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     2041 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     2101 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     2161 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     2221 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     2281 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     2341 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     2401 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     2461 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     2521 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     2581 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     2641 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     2701 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     2761 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     2821 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     2881 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     2941 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     3001 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     3061 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     3121 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     3181 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     3241 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     3301 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     3361 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     3421 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     3481 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     3541 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     3601 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     3661 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     3721 ggttgtgaca gaagggggca
//
LOCUS       DACEPD010000149         3737 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.158, whole genome shotgun sequence.
ACCESSION   DACEPD010000149 DACEPD010000000
VERSION     DACEPD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.158"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            103..1050
                     /locus_tag="IW797_002661"
     CDS             103..1050
                     /locus_tag="IW797_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAQ1225407.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            complement(1347..1895)
                     /locus_tag="IW797_002662"
     CDS             complement(1347..1895)
                     /locus_tag="IW797_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225408.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1896..2753)
                     /locus_tag="IW797_002663"
     CDS             complement(1896..2753)
                     /locus_tag="IW797_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1225409.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(3039..3326)
                     /locus_tag="IW797_002664"
     CDS             complement(3039..3326)
                     /locus_tag="IW797_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1225410.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(3316..3645)
                     /locus_tag="IW797_002665"
     CDS             complement(3316..3645)
                     /locus_tag="IW797_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1225411.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT         1143 a          678 c          530 g         1386 t
ORIGIN      
        1 aaaaaaatga tgcttatttt taaaaattta aaaaaagact aaattcaatt tttttggtat
       61 aatgagaaaa aatatctatt tgaaaaagtt aggagctata ctatgcatct ggaattctct
      121 gactatgaat cactccttgt aaaaatttgt gaagactatt atctcgagga tatcaatatt
      181 agtgaaattt ctaaaaaata tgggatcacc agatataaag ctttaaaata tattgaagat
      241 gcacgtgaaa aacaattagt tagtatttca attaattctc cgtatgctag gaattatgag
      301 ctagaagaaa tcttcaaaaa ttactttaaa acgaatatct atatattaaa aagtagtgaa
      361 gacttatcac aacacgattt attattttca aaatttgctg ccgattacat tcaagaatta
      421 attaagaaag ccaatgtggt tgcactatct tggggagata ccgtttacaa agtgattgat
      481 cagtttaaaa tgggtgctcg tgaagaatta atttttactc aattcattgg cgaaatcgga
      541 aaatataatt ccttagctgg ctccacacga ttagttcaaa aagctgctga acgttatgag
      601 tcttcttatt tgactctcag tattccttta tatgtcatca atgaaactgc acgtgaacta
      661 atggctctag aaccttcttt agctaatacc ttagcaaccg cttcgcatgc agatatgtta
      721 atttctggaa ttggcacctt atcctctatt gattctgtcg aaccctggaa caagcatcgt
      781 aaaatcctat ttccaagcat gacggatact gttggatttc tttacggaag acccttcaat
      841 atcaatggtg aattcatcaa agcatctgag gataagactt ttggtctttc tttaaaagaa
      901 attttcgata ttccaaaacg aattggtatt tgtaaaagta agttcaaagc ggagagttgt
      961 ttaggcgcct tgaatggtaa tttttttaca gatttatttt tggatgaaaa aactgcttgg
     1021 aaaatacttt ctttgataaa aaaagagtga gtcatgtaaa atcctccaaa aaatttagat
     1081 ttttggtact acttttttgg agggcagtac aatgattcac tctttttatt ggatatcaaa
     1141 atcccttttt ttgagaagct tgtcttgata ttttttgaac ttttccaagc catactccat
     1201 tacttttcaa gatttaattt ctttgtattc actgaataat ttccacgttt tttcgttaat
     1261 gttacttctt attaatttta ttgtacttct catgcaatag gatcatatat ccttctaaat
     1321 cttttttatg ctataaaaag ggggttctat ctaactttat tattcattcc ccaccctttg
     1381 cgcagtgagt cttcggtatc ttgttttatc tgactggtag ttcttattat ttctccacgg
     1441 tcatctaatt ctggaacaat tggatatttt tctctgaaca agtctagttc tttcaccacg
     1501 tcattaaaca aatcttgctt atcttcttgc gtaagattct gtttaatata ctcatctaaa
     1561 gcttttctaa gcaattcatc tcttgtcata ttagtctcat acttttctac taaaactctg
     1621 gaagtcgcat ttaacaaagt cgacagtttt aaaggatgct tggtggaata aaccttatca
     1681 aactttctga ttggtgcgcc aacttttttt gttcttcctc tactctgtct ttcaacattg
     1741 gaggaagttt ttatttcttg aacttggtgc acttctaatt gcttattttc agatggtgta
     1801 attgcttgct gctttttctt tgcttctgct tcttttttta ttttggcgag tcctttgttg
     1861 ttcccaaaaa tatcaagtcc tttgatgtca gccaattact ccacctccgc ataccaattc
     1921 tcgtgttctt ccaattcttt caaaatactg ataaataatt cgtggctttt gcgatcccat
     1981 tgttttaaga gacctttctt tgtcttctcc atcgtaatac ctgatccgtc gtatcgtttt
     2041 aagcgcgcat ttttgagtac aacattttta atcaaatgtt ccccataaat ctcttgggct
     2101 tgtttataca tttcctgatc tactgcgtca ccgctttcca acatgaaagg aataacacca
     2161 gcaacatata aatttgtatc aaattctttc acaaaaaagt ccatatactc aagatattgc
     2221 atcgcatttc tcatcgattt tacttgggtc tgtaagacga cgattgccca gtccgcaatg
     2281 tacatcgcgg atttactata tacagaaata gaaggtggga catctaaata gatgacgtca
     2341 aactcatcaa ataatggttc tagcttttct ttcatatatt tgaattggga taatgaatca
     2401 ttatattggt ctttgatcca atcatcatac atttcaaagg acatatctgc tggaagtata
     2461 tagaggttat tgattacttt atagacagcc tctttaggtg gaagattgtt tatcaacacg
     2521 tcagtcacag tattttcgta aacaatcggt tcaccttctc cgcctccttc ttcgttagtg
     2581 aagttcatat gcgttaagtt catcacatca gaagtatttc cttgcatatc caagtcaatt
     2641 atcaaggttc tttttccttc attaactgct gattcaaacc ctaacatttg cgcgcacgta
     2701 cttttaccta cgccaccttt aaaattacca attacatatt tcttcgctac cacgttccta
     2761 cctcttttct tttagcaata ttcatttgat actattataa catatttatt tttagttact
     2821 accaaaaaaa agatatatat gtactacctt ataattaata agtaaaaggt agtacataaa
     2881 taaggaaaag ttgaaatata ttgatttata gctttcctca aagtaatgac gttatttaat
     2941 ttatcgagaa gcagagaata ctcgcgactt tagtcatgta gacgaatcag attttcgata
     3001 aaaaagaagc cgtccttctt aagaacgaca acaaatcact agattaattt tttcaactgt
     3061 tcataaaagt tttcatgcgt tccacataac aatactggaa taaccttgtt ttctataatc
     3121 tcgtaagcta ctctatatgt tgtgcccgca tatttgaacc cactagacca aatacctgat
     3181 aaatccccag ttttttgttc acccttatat ggatcaatcg caatttcatc ataaattaaa
     3241 ttcaaaaatt tttctttcaa tggcttctct tttagctttt ttaaatactt aatgacatgt
     3301 tttgaaggaa cgatttcata agccaatttc tgttgccaac tcctctttag tcattacttt
     3361 agaattagta agtgtatctt cagcaatatc tctaaaggaa gaatgcattt tgttttttct
     3421 attcttaaat tctacaagaa tctcatcttt atcgtagcct tctgcaatta tatcagacaa
     3481 aatatcttcg ctaaaatcat aaaattcttt ttgtggttca acaaattcat aaagaatacc
     3541 attctctacc aactttgctt caacttcgac tccattagga acattaaaat ctttaggtac
     3601 tgtcaacatg attgaattgc cttgttttct tgtttttgta atcatttaaa tccctcattt
     3661 ctaacagtat gtaattactt agtaaataca ttataccacg agataatgta tttacaagta
     3721 attactgtga ctcgtaa
//
LOCUS       DACEPD010000150         3682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.159, whole genome shotgun sequence.
ACCESSION   DACEPD010000150 DACEPD010000000
VERSION     DACEPD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.159"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            238..561
                     /locus_tag="IW797_002666"
     CDS             238..561
                     /locus_tag="IW797_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225412.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            725..979
                     /locus_tag="IW797_002667"
     CDS             725..979
                     /locus_tag="IW797_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225413.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            1049..1294
                     /locus_tag="IW797_002668"
     CDS             1049..1294
                     /locus_tag="IW797_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1225414.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1391..1735
                     /locus_tag="IW797_002669"
     CDS             1391..1735
                     /locus_tag="IW797_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225415.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(1796..2359)
                     /locus_tag="IW797_002670"
     CDS             complement(1796..2359)
                     /locus_tag="IW797_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225416.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(2927..3127)
                     /locus_tag="IW797_002671"
     CDS             complement(2927..3127)
                     /locus_tag="IW797_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ1225417.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         1116 a          640 c          573 g         1353 t
ORIGIN      
        1 acgaccggac ggttatgagc cgtctgctct aaccaactga gctaaaggta catcataaat
       61 aaaatttatt tttgttactt ttcaactata gcacgaatga caaactctca caagtttcaa
      121 aatataagta aaataatatt gttattctta tttcttttac ttgtgtcata actattttgt
      181 tactttaatt tttatttgct tattttagaa aaaatgttat tcttttattt attctcaatg
      241 ttaggagtga ttattttgcc atttattgcg attttattcg atttagttgc ggcagcagct
      301 tattttctcc aattaaatta tcaaacctca ggaattctgt ttattggaat gatttttcaa
      361 ggtgtgatta cccttttgct tcttgtcatg atgctcagct ataaaggaaa aagatatgcc
      421 cgtattcaaa caaaagtatt tgttaaatat gtttccattc gctatgggat catcattctt
      481 tcctttttag tgaatgcgat cgtcctcttt ttatatgtat tgaattactt agatatcaat
      541 ccattgattt tttctcgata gataagacac attcagcgga acagttcctg cttatcagca
      601 gaaaactgtt ctgctttttc atttttcatt gatctattct tcttttattt tcctagcatt
      661 tcatttgtga tgtatgcaat aacatgttat cttttattat atgaatcgtt gaggagtccg
      721 ttacatgatt acgttggtga gagttttgtt ttgggtgcct gcggtcgcac tggtcgcaag
      781 tattgtttat cttatgaatt ggaacaaaga acggttttat ttagctattt tgactcttcc
      841 ggctatctac tttatgtgga aagtttttaa ttacaactac tttgaacccg atagtgtttt
      901 tatagaagaa ttatcaggac tggttttgtc tttattgatc gtcattcttt atttgatacg
      961 tttaaataaa aaacattgag tactattgat tgcatgtgta gctttctata gtcattataa
     1021 ctattctact ataatgaaag gtgattaaat gagtatcgaa gaagctattt tactggatac
     1081 tttacgttgt cgtctgaatt tcttaagaac tggccgatct ttatctcaag tagctgtttc
     1141 tagcgcgtta agtattcctt tattcaaata tcgtaagtac gaagaaggag aagaaattcc
     1201 aagcatcatt gaattaatct atatcgctga cttttacgat atttccgttg attacctaat
     1261 tggtcgctca gaaacagcaa gcaaactgaa gtgatttttt ccagccttgg tgctggtttt
     1321 ttcatttaac gtctaaggtt aacaatctat atataaagga gacatttcta tgtgattatg
     1381 gcatgaaaaa ttgatttcaa agcttcctcg tcaacagctt ttaggacaac atcgggaatg
     1441 ctgtgcatta agaggaaatg gctggggaaa gaaacatgct actgtggatt atgtttttca
     1501 ctattctcct tatagattat tccaatatca tcagcttgtc atggatgaaa tggaaaaacg
     1561 tggttacagc ccagcaccag agtggaaaaa tcctatgtat cgtggcaaat catgcgaagc
     1621 attttttgat ttacctactg tagagcgcaa cgtgccgatt tatccggaac acgatgaagt
     1681 ttatctagca gaatgtttag caaatttgga gcaaaaagga atttatctta cctaatataa
     1741 ctagaaaagc cttctatctc ctcttacttc attaaaaagc tttaaccatc ttagatcaag
     1801 ctaaaatgtc gtaagtttta gggttatctt catcctcacg tgcaaaacta aaagcgtact
     1861 ctagttttgg ttcatcgcca acttccctag acataaatga acatctcctg acgtggttta
     1921 aaagtaccgc atcttcgacg tctgtatacg atgcacagca cagattgctt cttttgatct
     1981 ttcttttgat ttttctgttg cttcttctac accttcttcg attactacat cgatgcttac
     2041 aattgcttct gcctaatatt cccatgcatc gtcgatttaa gcctctgtag tcactgtgat
     2101 cgtttccaca ttttaactcc tctgtcgtgg cttcagttga ttttgtgatc agttcagttg
     2161 ctaagccaga gtcagaacaa cctgccattg ctagcgttaa tattgatagt aaaaatagtt
     2221 tttttatcat aaggttcccc ccctgaaaat agactaaaat aataacgctt tctttttttg
     2281 ttttcagacg aaaattaata atttctaaat attctttaaa aaagcttata actattatgc
     2341 cttttttaat atataacaca gttattttta aaaataagaa tgctaaatca ctctacaata
     2401 acggcgaaag cacaaaaaat aaaaaaatta ttacgttatt aaaaaaacag atgaaagatc
     2461 aggttttatt agatctcatt ctttcatcac ttgaagggat ttaatgaact actttttcct
     2521 ctttgtcgtt tttatatatt cgtattcctt caaacagtag gatagcctct ttcaatgctt
     2581 gatatttgaa ctgtccgtta cttaaaaatt tagatgtgcc gccctcttcc tagaacatat
     2641 tcttttttac tttttcatta attgacagac caatcgaacg aaactgtaaa tttcgatagg
     2701 ataatgattg gtcaaactca ttcattttca agacatctcg attcatattc aagataggaa
     2761 tctttttatc aacatcactt tattcgtatt ttcctctacc ataaaagctt cacgctccac
     2821 aaaattgttt tatacactac tattcagccc attcaacgac caatgaattt tttcgtacaa
     2881 aaaaaccgtc ttttctttcg aaaagacggt taaaagtcaa tttttattaa gctttaacaa
     2941 tgtttgttgc ttgagggcca cgttgaccat cttcaacatc gtaagacact gcttggcctt
     3001 cttctaaagt tttgaagccg tcaccttgga tagctgaaaa atgtgcaaat acatcatttc
     3061 catcttcacc agtgataaag ccaaaacctt tgtctgcgtt gaaccatttt actgtaccgt
     3121 tattcataaa taaattcctc ctgatacgta tcaatgtacg ttttttcgtt gcaataaata
     3181 tttgataggc aaaaggagtt ttatctatgt agatatatcg ctcaaattac gtttcaaaat
     3241 agattactca tttaatatat catgcttttt ctaataacac aaggataatg caatgaaata
     3301 taaaattcat tgtcttctat tgtcttcgaa tcgaagaaaa agttgaataa ccgcgattga
     3361 ttcacttttt gttttcttgt ttatttgctt ttatctgcaa cttttgactt taaaatttct
     3421 taactatgaa aacctttcaa ttggtggtat acttataaat ggaatttcgt tatcaacgcg
     3481 ttcgttgcct ttcagcaagt atttaaagaa aggagaatgg ctatgacatt ctataacaaa
     3541 gatatacaat atcgagtaac cttggataca gatttgaatc tatttgtcgt ttttgacaaa
     3601 gaaaatacca atcatgtagc gacaggtgtt acgatcgaac aagcagtgca agaattaaaa
     3661 aaatctgcat aaggagtgga aa
//
LOCUS       DACEPD010000151         3549 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.160, whole genome shotgun sequence.
ACCESSION   DACEPD010000151 DACEPD010000000
VERSION     DACEPD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.160"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            20..1135
                     /locus_tag="IW797_002672"
     CDS             20..1135
                     /locus_tag="IW797_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ1225418.1"
                     /translation="MGGASIVASKIAKGLIDSGHEVFFVSYLESEKPQVESKYYDLYE
                     KKIFLFKNFSRFHKILEYRLFGGFVPSRYIKKEKEQLKKVLKKEKPDVIIFNTFIPSV
                     LFSEFLKYEFPTVKLITWMHSDPDYSLNHIAKFYKKEYRKSFERVDSIVCLSQDTKEK
                     LSLYNNHIKVIYNPLTLSGSSLISNLDKKIISFTARYDINIKGFDYLCEVAKNLPVGW
                     AIRIAGDGTEEERKELKRLIEKNGVLDKIICAGALFGDELINHYIESSIFISTSRSEG
                     LPLVLIEAMTYGLPVISFDHGGAKEILQDGNYGILIKNFDVQDMNNEIEKLSNNRLLM
                     EKYQKFSLERAKDFNEKKIIGEWQTLLNELGNEDVRN"
     gene            1132..2547
                     /locus_tag="IW797_002673"
     CDS             1132..2547
                     /locus_tag="IW797_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ1225419.1"
                     /translation="MRTSIKNFNLWKEWYLYIFFIGMIIRAINFYSLLPSTFDSIFFK
                     LIGISGVLLLGIDFIIKFNKRKVTYNILLIAYMVILVITSFVHKEYGLGENLKTIMWA
                     ILQYFLIYQFAFENSDNKRFFKRISWGFIYSWFVLVSMSMVLFFGKFGYEKYYNARHR
                     IRIGFLESRLFGTFSDINNAAIASLVVIVLILFYLYNKKVTGLNKIIFLINLPLQFIY
                     IVLSGSRSTYVVSIIIVAISSFIVVLKRNGTKKDLKNYILAIVSSIGSVLILFALFNA
                     TKILFQELLPHLKEINFFNFTERHKELMNNSQHINNTQTPDNTLVRKDVAENTDISNA
                     RITIWKSAWEIFKTSWLIGVSPKNVVSYAQSIIPDGYIAKVGIAIHNAYLNVLTSTGI
                     LGIIPFMAFLIKSFVLNVYRIIYNKCIGKDYHFVYCLVILSYALYAALNNELVYENTV
                     GTFVFWLFLGRVNSKNNSSYF"
     gene            2580..3527
                     /locus_tag="IW797_002674"
     CDS             2580..3527
                     /locus_tag="IW797_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ1225420.1"
                     /translation="MLNYLITGGAGFIGSTLANYLGKENNVVVIDDLSMGKKENLDES
                     KHITFIEGDVADKNLMEKVMKEYHFDYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
BASE COUNT         1249 a          441 c          629 g         1230 t
ORIGIN      
        1 actatctcca gctttctcaa tgggaggagc tagtatcgtg gcctcaaaaa ttgcaaaagg
       61 attaatagat agtggccatg aagttttttt tgtttcttac ttagaaagtg aaaaaccaca
      121 agtagaatct aagtattatg atttatacga aaaaaaaatt tttttattta aaaatttttc
      181 tcgatttcat aagatattag agtatagatt gtttggaggc tttgttccta gtagatatat
      241 aaaaaaagaa aaagaacaac taaaaaaagt tctaaagaaa gaaaaacctg atgtcatcat
      301 attcaatact tttataccta gtgtactatt ctccgaattc ctaaagtatg aatttccaac
      361 agtgaaatta attacttgga tgcactctga tcctgattac agcttaaatc atattgctaa
      421 attttataaa aaagaatata gaaaaagttt tgaacgcgtg gattccattg tttgtctttc
      481 gcaagataca aaagaaaaac tttcattata caataaccat attaaagtta tttataaccc
      541 attgacatta agcggctcta gtttaataag taaccttgat aaaaaaatta tttcatttac
      601 tgccagatac gatataaata ttaaagggtt tgattattta tgtgaagtag ctaaaaactt
      661 acctgttgga tgggctataa ggattgctgg agatggtaca gaagaagaac gtaaagagct
      721 gaaaagatta attgagaaaa atggtgtgtt agataaaatt atttgcgcag gtgcattatt
      781 tggagatgaa ttaatcaatc attatataga aagttctatt ttcatatcta cttctagaag
      841 tgagggacta ccattagtgt tgatagaagc aatgacttat ggattacctg taataagttt
      901 tgatcatggt ggtgctaagg aaatattaca agatggaaat tatggaattt taattaagaa
      961 ttttgacgta caagatatga ataatgagat agaaaaattg tctaataatc gtttacttat
     1021 ggaaaaatat caaaaattct ctttagaaag agctaaggat tttaatgaaa aaaaaataat
     1081 tggtgaatgg caaacattgt taaatgaatt aggaaatgag gatgttagaa attgaggact
     1141 tcgattaaaa attttaattt atggaaagaa tggtatctat atatattttt tatagggatg
     1201 attattcgtg caataaattt ttatagttta ttaccgtcca cttttgatag tatttttttt
     1261 aaacttatag gaatttcagg agttttgcta ttagggatag atttcataat taaatttaat
     1321 aaaagaaaag taacttataa cattttattg atagcatata tggtgatttt agtaataacg
     1381 agttttgttc ataaagaata tggtctagga gaaaacttaa agactataat gtgggcaatc
     1441 ttacagtatt ttttgattta tcagtttgct tttgagaata gcgataataa acgatttttt
     1501 aaaaggataa gttggggatt tatttatagt tggtttgttt tagtatcaat gtcaatggtt
     1561 cttttctttg gtaaattcgg ctatgagaaa tattataatg ctagacatag gatcagaatt
     1621 ggttttttag aatcaagact gtttgggacg ttttctgata ttaacaatgc tgctatagca
     1681 tctttagttg ttattgtcct aatcttattt tatctttata ataaaaaagt gacaggatta
     1741 aataaaatca tttttttaat taatcttcca ttacaattta tttatatagt attaagtggg
     1801 tcacgatcta cctatgtggt aagtataata atagttgcca tatcatcttt tattgttgtg
     1861 ttaaaacgaa atggaacaaa aaaagattta aaaaactata tccttgctat agtatcttct
     1921 attggttctg ttctgatttt attcgctttg tttaatgcaa ctaagatact ttttcaagaa
     1981 ttattacctc atttaaaaga aataaatttt tttaatttta cagagcgtca taaagaatta
     2041 atgaataata gtcaacatat taataatact caaacacctg ataatactct tgttaggaaa
     2101 gacgtagctg aaaatacaga tatttctaat gctagaatta ctatatggaa aagtgcatgg
     2161 gaaatattta aaacaagttg gcttatcgga gtctctccga aaaatgtagt atcttatgca
     2221 cagtcaatta ttccagatgg atatattgcg aaagtaggaa ttgccattca taatgcatac
     2281 ttgaatgtat taacatcgac aggtattcta ggaataattc ctttcatggc ttttttaata
     2341 aagagttttg tgctgaatgt ttatcgaatc atctataata aatgcattgg taaagattat
     2401 cattttgttt actgtttagt tattttatct tatgcactat acgcagctct taacaatgaa
     2461 ttagtctatg aaaacactgt aggaacgttt gttttttggc tatttttagg aagagttaat
     2521 agcaaaaata attctagcta tttttagtaa taaattttat cttgaaaaga ggagaaaaaa
     2581 tgttgaatta cttgattacc ggtggcgccg gcttcattgg ctcgacgctc gcgaattatc
     2641 taggaaaaga aaacaatgta gttgtgatcg atgatttatc gatggggaaa aaagagaatc
     2701 tggatgaatc gaagcacatc acgtttattg aaggggatgt cgctgacaaa aatttgatgg
     2761 aaaaggtcat gaaagaatac catttcgatt atattttcca tctagctgct gtagctagtg
     2821 tggcagattc tgtagaacgt cctctagaaa ctcatcgtgt aaattttgac agtgcgcttt
     2881 tattgcttga attggtacga aaatatcaat ctggtttaaa acgattggta tttagttcat
     2941 cagctgcagt ttatggcgat gaaccaacgt tgccaaaaaa agaagaatcg gtcatccgtc
     3001 ctttaacacc atatgccatc gataagtttg cagcagaaca atatgttttg gattactgtc
     3061 atttatatga tgttccgact agtgctgtcc gattttttaa cgtttatggt ccaaatcaaa
     3121 atcctaactc tccatatagc ggagttattt cgattctagt ggaccgatac aaaaaacaat
     3181 tggcaggaga aaaaacagaa tttactttat ttggtgatgg tagccaatct agagacttcg
     3241 tttatataga agatgttata caagcacttt tacttgttgc taaggaagaa aaagctttag
     3301 gacagcaatt caatgtcggt acaggaaaaa gcactacgct acttgaattg attcattcaa
     3361 ttgatcaaat cttaggaaca gagctagcgc ttaaatatga agcggagcgt tcaggggata
     3421 ttcgtgattc tttagcggat atttcaaaga tccgttcttt aggttatcaa cccaaatttg
     3481 atatattgaa cggaatggaa cgttatttaa agacagaaat cgattgaaac aaaaaaccag
     3541 catcctgct
//
LOCUS       DACEPD010000152         3530 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.161, whole genome shotgun sequence.
ACCESSION   DACEPD010000152 DACEPD010000000
VERSION     DACEPD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.161"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(27..530)
                     /locus_tag="IW797_002675"
     CDS             complement(27..530)
                     /locus_tag="IW797_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ1225421.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(653..1417)
                     /locus_tag="IW797_002676"
     CDS             complement(653..1417)
                     /locus_tag="IW797_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ1225422.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSADQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1524..1799
                     /locus_tag="IW797_002677"
     CDS             1524..1799
                     /locus_tag="IW797_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ1225423.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            2515..3465
                     /gene="yidC"
                     /locus_tag="IW797_002678"
     CDS             2515..3465
                     /gene="yidC"
                     /locus_tag="IW797_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ1225424.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         1071 a          722 c          668 g         1069 t
ORIGIN      
        1 atatggtcta ttattggttt tacctgttaa atcgaggatt ctttcccaaa gtaacgttgc
       61 catctcttac gcatacttgc agacatgggc tgagccgctt ctttgactgc gcagtatttg
      121 tccacgaatg tcacaatata tccttcttta tacttaggcg gtgcgatcgt tgcaccccac
      181 atgtgcttca aaatgatatc tcgctcaaga tccgacaaat cggtgatttt ttctgcattc
      241 tttacagcaa ttcttggatg gatatacgca tgtgtcccct cgtcaaattt cgtcgtacgc
      301 caatcatagt agaacagatc atgcaaaagt ccagcacgcg ctgttgcttt agcattgcca
      361 ccccattttt ttgcgagcag ataactatag taagaaacac tgatggaatg ctccaaacga
      421 gtggaatgaa catgctgtgt gtaattcgct aattttttca ctgcttctgt ttctaaaaga
      481 tcttgtacat aagacatata ttcttcgtct tcacgccatt tttctgtcat aattatcgat
      541 ctgccacctt tttgaatttc attttcttct gaaattgatc taagtatacc accagtgttc
      601 ggattattct agtcgctcta gataaaatag ctaaagctta atagttcctt aatcataaag
      661 aaaaatgata aagaacgatc cctgctgcga ctccgacatt caatgattcc gcatttcctt
      721 tcattgggat atatacgttc tgatcagccg aagacaagat ttcctgactt actccctgac
      781 cttcgtttcc catgattata gcgaaatcct ctgttttttc tactttgtct aatgcatgtg
      841 cttcttcatt taattctgta ccgtacaccg taaacccttt gcctttcatt acagggatca
      901 cttctgccaa atctttttgt aacacaggca aatgataatt gcttccctgc atgcttcgca
      961 gcacctttgt actataaata tctgccgttc ctttacctaa taaaacacca gccataccaa
     1021 acgcatctgc cgtacggatc atcgttccca catttccagg atcctgtaca ttatctagta
     1081 aaagccagcc tcctgaaaaa tctggcgtat cttcagatgt taacgtcatc actgctaaga
     1141 tcccttgcgg tgtaggtaga tctgacatag cggtcatgac ctcatctgaa actagaaaag
     1201 tagaaatctc tttgtttaca gccagccagt ctccccattc tttcatacct ctctgtgtca
     1261 caaaaagttc ttttatttct gcatcagacg acgcagcttc ttcgatcaga tggaaccctt
     1321 ccaaaagata ttctgccttt tcttctctgt atttcttttt atgtagtttt tttaattctt
     1381 tgattcttgg attttttact gattggatct ctttcacatt cctcacctca actgattata
     1441 tcatggaata ggtgcttgag cttgtttcat cctaaaaaaa tcggtatcat tgatttataa
     1501 aacaggaaag aaaggtgacg aacatgcgca aagttaaaat gaatgtccaa ggacgtgtcc
     1561 agggcgtggg atttcgctat atgaccaaga tggttgctga tcagttaggc gtgaccggaa
     1621 cggtaaaaaa cgaagacgac ggctcagtca caatcgaagc aatcggaaat gacgacatca
     1681 tccaaaaatt tatcgaagaa gtcaagaagt ctccgagccc aagtggacga gtgcagtatg
     1741 tggatgtcca agaagatccg atgattgagg aacgaaagaa atttgatgtg gtgggataag
     1801 gttgtttttg aagcgctttg cctcgagtat taaggagaaa cattttccaa atagcttctc
     1861 atattttgaa atgtaatgac ttaataccga gaggcagctg ataaaacacc gtcaataagg
     1921 ttgtgaaaaa gctggtctgc atcaagcatt aaggactggt tgtgaaaaat agctctccat
     1981 atttttcaac aggctggact taatgccgta gatgcaagct tttgaacacc gtcgataagg
     2041 ttgcgaaaaa gctgatctgc atcaagcatt aaggactagt tgtgaaaaag tcgcttagtt
     2101 ttgagtatta aggaaaattt tgtgaaaatg gctttccaca tttttgcata aatttgactt
     2161 aataccaaga aactgacttt tgaacatcgt cgataaggtt gtttttgagg cactttgcct
     2221 caagtattaa agacaggtag taaaaaagct gatctataac ttgcaataaa tttgcttaac
     2281 ccatattttc cagacagaac aaaaaagttt ttagtttttt aaataagaaa gaaacacttg
     2341 gaatcactct tttctttcta agtatttctt tcttgatttt ttgtcttttt tcaaaatttt
     2401 tcgctacgaa aaagaacgaa tatttatatg aaatttctat gaagttgatt gaattccatg
     2461 cgctttttta gtatagttaa gtttgtgtaa aaattataga agaggaagta ttctatgaat
     2521 aaatggaaaa actggttatt gggatccgga cttgtcagta tcctgctctt tttatctggc
     2581 tgtgtgcgga tggattcaaa cggaaaccct gacacttcag gaatcgttta tcgagtactc
     2641 gtccatccta tgggccaagc gattacatat ctagttgaga atttcaattg gtcttacggc
     2701 tgggcagtta ttgcaatgac tgtgatcgtt cgtatcatca tcttgccact aggtatcagt
     2761 caatcgaaaa aaacaatgat ccaatctgaa aaaatgcaag cgttaaaacc acaagttgaa
     2821 gcagcacaac aaaagctaaa agctgcaaca acacgtgaag aacaaatggc agcacaagca
     2881 gaaatgcagc aagtatatcg cgaaaatgga ttgagtatga ccggcggaat cggctgtctg
     2941 cctctattga tccaaatgcc gatcttctct gccttgtatt ttacggctcg ctacacagaa
     3001 ggtatccgtg aatcatcttt ttacggcatc gatctaggct caccaagtat ggtgcttgtg
     3061 gccatcgcag gggtcgctta tctgcttcaa ggatatattt caacaatcgg tattcctgaa
     3121 gaacagaaaa agacgatgcg tacgatgctg atcgtctctc ctgctatgat cgtcttcatg
     3181 tcgatcagtg cgccagccgg tgttacactt tactgggtag tcggtggtgt gttcagctgt
     3241 ctgcaaacat tcatcacaaa cgttataatg aaaccgcgtt tgaaagcaca agtggcagaa
     3301 gagttgaaac aaaacccgcc aaaacaagtc gttactccaa gaaaagacgt gacagaatca
     3361 gcaaagccag ctgacaacaa acaagcaaca aaacaaatca aacaaccaaa aaacagtaca
     3421 aacaacaaag gccgcaacgc aggaaagcag cagaagcgga aataaaaata tatgacaaaa
     3481 aactgtagac aaataaaaaa atttctttgt tctacagttt ttttgttaca
//
LOCUS       DACEPD010000153         3518 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.162, whole genome shotgun sequence.
ACCESSION   DACEPD010000153 DACEPD010000000
VERSION     DACEPD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.162"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            38..589
                     /locus_tag="IW797_002679"
     CDS             38..589
                     /locus_tag="IW797_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAQ1225425.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1265..1531
                     /locus_tag="IW797_002680"
     CDS             1265..1531
                     /locus_tag="IW797_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225426.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1589..2101
                     /locus_tag="IW797_002681"
     CDS             1589..2101
                     /locus_tag="IW797_002681"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1225427.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2098..3135
                     /locus_tag="IW797_002682"
     CDS             2098..3135
                     /locus_tag="IW797_002682"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1225428.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            3295..3471
                     /locus_tag="IW797_002683"
                     /pseudo
     CDS             3295..3471
                     /locus_tag="IW797_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT         1288 a          600 c          697 g          933 t
ORIGIN      
        1 gagcgagcaa gacaggaacg agagaacaaa catactcatg aaaagactag aggcttcgac
       61 ctcgaacttt aataataagt tagatgtttt tgaagaaaag actaagcatg caagattaaa
      121 ctttgaaaca actgcaaagc actatattaa acgacttgat gaagataatt tgaaattaga
      181 ttttcaacaa gctattcaag atgaattaag cgatactaaa gacgaaatac gtgaagtgac
      241 gaaacaagcg agagaagaaa caaaagagta taaagaaatc ttagaaagta aaataaaaga
      301 ccataacaaa gtggttgata aatcgaatac agcgctaaaa gtgatgacaa aaggcgttac
      361 aaatatattt tttgtgctaa ttatttttgt acttgtaatg cttgtgacag gtccgatagg
      421 tcatttcttt ggtattgaac atttatatag ctttattaat ggttttattg atgaccacga
      481 aagtgcatgg cgatatttga tgttgatact ttatgcagta ccctatgtat tcttcgctat
      541 cattctgtgg ctcatagcat tgttttttag agtatttaat gacctttagg tagatttata
      601 tataaaaagg gctaaatacg caaaataccc ccttaaaatg caaaataaat gcaaataagg
      661 gggtaactcc ttatgaaaaa acaactaata gtattataac acgtttttat gccgagaaaa
      721 cttattggtt ggaatgggct atgtgttagc taacttgtta gcgagttggt tggacttgaa
      781 ttgggattaa tcccaagaaa gtaccaactc aacaacacat aaagccctgt aggttccgac
      841 caataaggaa attggaataa agcaacgaaa ggagttgagt aaaatgagtg agaaaacgaa
      901 gaaaatcatt cgagtatcat tagctttttc acaagtagta gtgcaatcat tgatagttta
      961 ttatacttac cagcaatata aattatctaa agagaattaa gttttgatta tgtaataagt
     1021 tttggtacta aaaaagactt gatctaatta gaccaagtct tttgttagtg ttatattaat
     1081 aacaaaataa aaataagaag tcgctcactc cccgaccaaa gtttgtgagc aaaataaaca
     1141 gtcgatgttt gtttcttaat tgtatatctt gatattaaac gatttaagat tattcttcaa
     1201 gatatatatt cgcaaatgta attggaaaag cgacttctta aacaaaatta aggagtcgct
     1261 ttttatggca aaaataacga aaaatgagca agaaaatttg gtggaaaatt acacaaaaaa
     1321 accggcgtac tctaatagcc ggttagacgc acatttcgtg tgcacttcta atccgaaatt
     1381 gagctttgac gctatgacga tagtcggaaa ccttaataaa aacagcgcta aaaagctatc
     1441 cgattttatg agtttagatc cacaaattag actttgggat atacttcaaa caaaatttaa
     1501 agctaaagca ctacaagacc ccctatgtta ggctagttgt cgtataggat aataagttat
     1561 aataaagact atacggagga tgattaccat gacaaataaa aacaatgaac gttactctac
     1621 cgaactgaaa gcgacagcca tcaagaaaat gatgccaccc gaaaataggg ggattcgcca
     1681 attaagtcaa actttgggca ttccagaagc gactctttgt tcttggcgta aaaaagctag
     1741 aattcaaggg aatactacac ccggaacgaa aaaaaatgtc gaaaaatggt caagtgaaga
     1801 taaattttta attgtgatgg aatcattctc aatgaaccaa gtagaacttg cggaatattg
     1861 ccgaaagaag ggcttattta aagaacaaat tgaggcttgg aagaaaacgt gcttatctgc
     1921 gaatgaacag gaagaaaatc gaacgagaga acttgcaact gaactaaaag aagagaaaaa
     1981 acaagcgcgt caattagaga aagaattacg tcataaggaa aaagcattag cggaagccgc
     2041 tgctctctta ttactaagaa aaaaagcaca cgcgatttgg ggggaccaag aggaagaatg
     2101 atttcatctt cagatcgcaa acaagctgtt gaacttattc aagaagccaa tcgcaatggg
     2161 gcccgtctca cctatgcctg taatgagctg aatattagcg ttcgaaccta cgaacgatgg
     2221 acgcgtgaag gtacgatagc gcatgatcaa cgccctttgg cgaaacgtcc tgtacccaaa
     2281 aataaactga cggatcaaga acgcgaaaaa ataatagaaa ccgtttcaaa aaaagaattt
     2341 atgaatttgc cacctagcca aattgtacca aaacttgcgg attgctcgat ttatattgca
     2401 tcagagtcta gcttctatcg tgtattgcga gaaaaaaata tgcaacatca ccgtggccga
     2461 agtcaagttt cacaaaaacg cataccgcca agtcaccttg cgacgaagcc aaatgaagtt
     2521 tggacttggg atattacttg gctaaaaggt ccaataaaag gactctttta ccggttatat
     2581 ctcatcattg atttatttag ccgtaaaatg gtaggctggg agatttggga atcagaagaa
     2641 gctcgtcatg ccgaaaaact tattaaacag acggtgctta aggaacgtat ccaaggacag
     2701 ccgttggttt tacactccga taatggcagc cctatgaaag ccgcgacttt tcaagtctta
     2761 ctcgaaaaat taggaattca aagttcttat tcaagaccac gagtgagtaa tgacaatcct
     2821 tattcggaag cggctttccg aacgttaaaa tatcgaccag aatatcccaa taacggcttt
     2881 aaaacaatag atgatgctcg aaaatggacg aaagagtttg tcgattggta taacgattct
     2941 catttgcata gtgggattca atttgtgtct cctgcagcgt gtcatgaagg acgacatctc
     3001 gctattttag agaaacgaaa gcaggtttac gaggacgcta aaaagcgcca tcctgaacgc
     3061 tggtcaaaag gtgttcgaaa ttggactcca catcaatcgg tggcgttgaa tcctttaaaa
     3121 gaaacagaaa gttaacagga gcataatctt tcaagattat caaataaccc tcgtcaccca
     3181 ataggggaaa aagttattct tcccctattg gataacgagg ccaaccgacc atagggaggt
     3241 tctgcttttt gataggtgga aaataaaaat acgacaacta tattgacaaa caccgaccat
     3301 cgtccagtaa agagacgcaa taaattctat cgaagtttac gcactgcctc tacaacgatt
     3361 aaaggcatgg aagccatccg aggattatat aagaaaaccc gaaaagaagg cactctcttc
     3421 gggttttcgg tctgtactga aatcaaggta ttattgggaa tcccagctta aatcatagat
     3481 accgtaaggg attttattct ttatttaaaa ctttgcaa
//
LOCUS       DACEPD010000154         3515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.163, whole genome shotgun sequence.
ACCESSION   DACEPD010000154 DACEPD010000000
VERSION     DACEPD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.163"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(23..637)
                     /locus_tag="IW797_002684"
     CDS             complement(23..637)
                     /locus_tag="IW797_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1225429.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIITSVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            951..1373
                     /locus_tag="IW797_002685"
     CDS             951..1373
                     /locus_tag="IW797_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225430.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1383..1586
                     /locus_tag="IW797_002686"
     CDS             1383..1586
                     /locus_tag="IW797_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225431.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1797..2381
                     /locus_tag="IW797_002687"
     CDS             1797..2381
                     /locus_tag="IW797_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1225432.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            2550..>3515
                     /locus_tag="IW797_002688"
     CDS             2550..>3515
                     /locus_tag="IW797_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ1225433.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSIL"
BASE COUNT         1182 a          559 c          660 g         1114 t
ORIGIN      
        1 ataataaaag cagcatgatc aactatttgt cattgaccat gctgtctttt tttattctat
       61 acacggtttg tctcgtaaca ttgtagtctt tagcaatctt agcaatcgca acaccatttt
      121 taagatcttc aacgatgctt ttgtaaacaa ggcgacgttg gggatctttt gcgtctgcac
      181 tgtataattt gggccgccct ttgtagacgc cgtttgcttt tgcgatctta attccttgtg
      241 cctggcgacg tttcgattca gtccgttctt gttcagcgat catggccaaa atttggatga
      301 tcagatcctt aatgaattta tccaggagag gattgccaat tgcttcagcc atgatcggta
      361 aactagtaat gatcagcttc acatttttct tcttcaagta attgactgaa gtgatgatct
      421 cgtcataatt tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga
      481 cgtaatctaa tgcttcttgg aaaacaggtc gatgagtaat cgttgcgcca gattgtttct
      541 cgatgaaaat tctctctgca ccgaattttt tcatttcttc aatctggcgt tgttcatttt
      601 gatcgatcga tgagacacgt atgtatgcaa ctctcaaaac ttttacctac tttctgcaac
      661 tatttaaaat acaccctaat catacacgag gaagccatat ttttcaatgt tataccagtg
      721 tctatttagg gtaaacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca
      781 cttttctttt taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg
      841 attatgacat taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta
      901 agatttatta agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata
      961 agtttatttt atctttaggt attttaacta ttggaattag tgtaactgga actacaattc
     1021 ctacatttgc tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa
     1081 ctcctttaga taggaacgga atagccattg gtaacccaac taacgactta actgttactg
     1141 aaaattattt aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga
     1201 caggaacgat gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt
     1261 ggaaagaaaa aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat
     1321 cagctggaaa caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt
     1381 ttatgccaga atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa
     1441 ttaatttacc gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa
     1501 aaattcaaaa atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc
     1561 aaaaaacata tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct
     1621 ttttttctaa ttgaaagtgt tcataaaatc acccatttct ggacactttc tttttttgct
     1681 ttatttttca aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca
     1741 gttaaactat atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga
     1801 aaataggata cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc
     1861 taaataaatt tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc
     1921 gaccaggatt agaaatggcc attgattttg ttcgctctgg cgatacactt gtcgtttggc
     1981 gcttagatcg tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg
     2041 agcgtggtgt gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta
     2101 caggacagct catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc
     2161 tggaacgatc cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc
     2221 ctgaaaaatt gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac
     2281 ctattaagac tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg
     2341 caaaaatgga ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg
     2401 aaaatttttg ccatttatct agaaaagtga caatagtatg agaatttttt gtaagtgtct
     2461 actgataact tattctcaaa tatttagcta tttcttcttt acaacatcga acatatgttt
     2521 gtataatgtg gaagaatgga gtgttattta tgatggatta ttcaaaagag ccagtaaatg
     2581 attacttttt aatcgatatg aaaagctttt acgcaagtgt cgaatgtata gaaagaaatt
     2641 tagatccatt aacaacagaa cttgttgtta tgagtcgatc tgataatact ggttcaggat
     2701 tgatattagc ttcttctcct gaagcaaaaa agcggtatgg tattacaaat gtgagtagac
     2761 cacgtgactt accacaacca tttcctaaaa cactacacgt tgttccacca cgtatgaacc
     2821 tatatatcaa gcgaaatatg caggtaaata atattttcag aagatatgtg gctgatgaag
     2881 atctactgat ttactcgatc gatgaatcga ttttaaaagt gacaaaatca ctgaatcttt
     2941 ttacgactga agaaacacga agtcagcgaa ggaagaagct cgctcaaatg atccaagaac
     3001 gtattaaaga agagttaggt ttgattgcta cggtaggcgt cggcgataat ccccttttgg
     3061 ctaaattagc cttagacaat gaagcaaagc ataatgaagg atttattgcg gaatggacgt
     3121 acgaaaacgt tcctgagaaa gtatggaata ttcccgaaat gacggacttt tggggaattg
     3181 gttcgcgaat gaaaaaaagg ctcaaccaaa tgggaatttt gagcattaga gaccttgcta
     3241 actggaatcc ttatacgatt aagaatagat taggtgtgat tggattacag ctgtacttcc
     3301 atgctaatgg gatcgatcga acagatatag caatcccacc agaaccgaca aaggaaaaat
     3361 catatggaaa ttcacaagtg ttaccacgag attatactag aagaaatgaa atcgagctgg
     3421 tggtaaaaga aatggcggaa caagtagcga tacgcattag gcagcataac tgtaaaacag
     3481 gttgtgtcca tttacacatt ggcacgtcga tctta
//
LOCUS       DACEPD010000155         3398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.164, whole genome shotgun sequence.
ACCESSION   DACEPD010000155 DACEPD010000000
VERSION     DACEPD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.164"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..276
                     /locus_tag="IW797_002689"
     CDS             <1..276
                     /locus_tag="IW797_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAQ1225434.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            419..640
                     /locus_tag="IW797_002690"
     CDS             419..640
                     /locus_tag="IW797_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1225435.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            656..1498
                     /locus_tag="IW797_002691"
     CDS             656..1498
                     /locus_tag="IW797_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225436.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1561..3363
                     /locus_tag="IW797_002692"
     CDS             1561..3363
                     /locus_tag="IW797_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAQ1225437.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
BASE COUNT         1228 a          469 c          703 g          998 t
ORIGIN      
        1 gaactcatca agctggctaa taagggtcaa gattatgaac atctagcaga tgagattgat
       61 gagctgagag acaagcgaca gctcctttta gtagaagatg cctccctcag tggcgagaac
      121 gagcgaatca atgagctgat tgaatttatc cgcaagaaca aattccgtac cttagagtac
      181 gatgataagc ttgtaaggaa gataatccag agcgttacag tctatgaaga acacttcgtc
      241 atatccttca aatctggcat cgaaatggaa atatgaaaac cagaagtaaa tagcccatga
      301 ttctacagta gagtcgtggg ttttcttttg cttgctgatt ataataatat tttaataact
      361 attgttatta taaaccaaat gatttataat attcttagcg taaacttgga ggtgctgtat
      421 gactacggca gaaatgatta aagaactgtg tgagcaaatg aatataagtg tttccgaact
      481 tgctagacgt attggacaga ctccacagaa tttcaataag aaattacaac gagaaacggt
      541 aaccttagat gagttgaaag cgattgctga tgtgctaggt gtcaagtttg tgcaggcttt
      601 tatattgcct gatggcaatg aaataaagat aggtaattag gaggtgtaat ttgtattgaa
      661 aatcataaca ataaatgatg ttgagtatgc tgtatttgct gctaatgaag gtacttccaa
      721 accacaacct catatcatag aaacgaaatc tggtactatt ccagaaggaa aacaactgag
      781 tttattaaaa gaatatctta agcagaatga tatttcccca ataaaaggag caacaacata
      841 ttggtgcatt gacaaggttc ttaagcttga ttcaagtaaa gaaaaaacta taagtgaaac
      901 aatccataaa caaaaatact tatctttaac tgaagaaaat attgagaaac agcataagtt
      961 tgtaggtgca agctctaatt atgggaaaga aggtctaata atacatgatg ttttgaatgc
     1021 atttcctctt cataatgatt taaatactat tgcgatgaag attgcggtaa ttgatgtaac
     1081 aaattcaaca catctttctc aatataagag caggttgtct ctatatgatt tggctaaagt
     1141 aattctggag attccaaatt ttgatgatcg gttggcaaaa ggagatcctc agttgataaa
     1201 tattatcgca aggaacattg gtgcagttaa tatgttctca tttgcatcca agtactgtac
     1261 atatcataat gtggaagtgt gtgggagaga tgattactcc atctttgatg gtatcgttaa
     1321 aaatactctt ccacactata tccaaggact aactacgaat aagattgata cgtggcgaag
     1381 aagttttgat tacgaggcat ttaacgaatg tgttggaaaa ctacttgatg aaaataatat
     1441 acatattcct tttagaagaa gaaagttaga tcacttcttg tggtatgcga ataggtagaa
     1501 tataagaaaa aggcatcgta aaagtgattc tggataaaat taatggggga ggaaataata
     1561 gtgggattat ttgattttct aaagaaaaaa caaggaaagg ttagtaaaca cgttgattat
     1621 caactggctt ctagtctatt tcgtactggt aaagtttttg ctgaaaaatt ggaagtggac
     1681 atatcaaatg tagatgagtt tcgtaaaact tttatcgctt ttgatgtgga aacaactggc
     1741 ctaaattctt cttcagatag aattgtagag attggtgcgg tgctatttaa agaaggagtt
     1801 ccaattcaat cattcggaac tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg
     1861 gcaataaata atataactaa tgaaatgcta aactctgctc caaatgagca ggaagtttac
     1921 ccccagttgg tagaattttt aggagatgca ctagatggaa aaatcactat gtgtgcacac
     1981 aatgcaagtt tcgattttgg atttttaagt aacacatttt ctagacttgg gtatgatgct
     2041 aaaattaagt atgttgatac tctgagtctc tctagaaaac atataaaagg cattgtaaat
     2101 cataagcaag agactgtagc ggaatatttc ggattaataa atgacactgc ccatagagcg
     2161 gaatcagatg cagatatatg cggaaagatt ttttggggtg ttttagatat tattgaggag
     2221 tcgttggaag aacaaaaaaa gcaaatagaa aagatgactc cagacataaa agaacttgaa
     2281 gcttgtgctt ttattcaaga tattattgag aaaaaaggat gcgacaccag ttggatcagg
     2341 tatcgaaaaa acagtaataa ttatgtcgat gtaacttgtc tatatacatt cttaaagttt
     2401 aagtttgcta agaagggaaa ttacatcatt attagaaaag atgctacaaa tggaattgat
     2461 ttacctatag aaccttgtac tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt
     2521 cgtagcccct ttgacttaac gcctctttca aattatattt tcagtgctta tagcgattgt
     2581 tataattcta tgaatgaata tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa
     2641 agccttagta tgctgaaatc tatttcaaaa tcagcgatga aagagttatt attcgatgca
     2701 aaaaatcgtg gatatgatga gactgaagtt aacctaaata ttgaaaaagt gatatctaga
     2761 gaagatgtaa ttattaacgg gttgcataat cgagtttcat taaacaagat acgaaatata
     2821 gggaattggg agaaaggatt tgatgcaggt tatccatatt gggaacgtgg agaaactgct
     2881 aggaaaaatg gcaaagtaga tgaagctatt gcactttttg ataaagctcg atataacggc
     2941 tatgatgctc ctgcgctata tgattcttat gctaaagctt atcgaaaaat taaggattac
     3001 gataatgaaa ttctcattct tgatgaagga attatgcgta aaacacggca cgatgttggt
     3061 actttagagg ctcgtaggga taaggcaatt aagctattat ttgctaaaca agaggcagag
     3121 cggatagcta aggaaaaatc tgattttcta aaagcgaaca aaaaagagga tgcgattagt
     3181 gagcctagca agaagcgtgg aagacagatt attcaaatga ctgatgatgg aacaataata
     3241 aaagaattcg atactatagc ttctgctgta aaagaagttg gcgttaactc aaaaagtatt
     3301 agggatgctg caaatggagt tcaaaaacat gctggaggat actgctggaa atacaaagat
     3361 tagcagataa tctaagataa cctaggatct tcttaata
//
LOCUS       DACEPD010000156         3392 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.165, whole genome shotgun sequence.
ACCESSION   DACEPD010000156 DACEPD010000000
VERSION     DACEPD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.165"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            210..476
                     /locus_tag="IW797_002693"
     CDS             210..476
                     /locus_tag="IW797_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ1225438.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            503..802
                     /locus_tag="IW797_002694"
     CDS             503..802
                     /locus_tag="IW797_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAQ1225439.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            976..1527
                     /locus_tag="IW797_002695"
     CDS             976..1527
                     /locus_tag="IW797_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ1225440.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1673..1966
                     /locus_tag="IW797_002696"
     CDS             1673..1966
                     /locus_tag="IW797_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225441.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1959..2255
                     /locus_tag="IW797_002697"
     CDS             1959..2255
                     /locus_tag="IW797_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ1225442.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2330..3328
                     /locus_tag="IW797_002698"
     CDS             2330..3328
                     /locus_tag="IW797_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1225443.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
BASE COUNT         1278 a          461 c          667 g          986 t
ORIGIN      
        1 atttagaaaa acttactaaa caaagagata tgaaaaactt taataattgt gtagttttga
       61 taattataac tgaattaact ttatttacaa atatatatta tatccatgaa gcccaatctt
      121 ctttaataga agattgggct atttttatgg caaaggaggg aaaagaaatg tttcaaaata
      181 aaggataacg ttatgtcaca aagggagtaa tggacagttt gccggtggaa ttacaagctt
      241 tatgttggaa tctaatcgat caaaacgttc aaaagcaatt tcctttagac tatcttcaaa
      301 tttttgaatt ttctacggaa aaaggaaatc aaaagttggt tcatcgtcaa gaagaaccgg
      361 aagaaagaaa agagtatctg atttcaccta agcttcgttt gaaatcagtg tctcaaaaaa
      421 tatgggccat tgattcgggt gagtatcaaa cgatgttatt accagaagaa tactagtatc
      481 taaaagaaag gaacgaaagg acttgttaga aacggctatc gaagtactgg aaaagtgtgc
      541 acagctggtc actgcttctg aagagtgggg ctatgaatcc gttacgatgg aaaaagagga
      601 aattgaaatg ggtatgcttc ctgaagatgt tcatctacca cgtttagtga tgacacatct
      661 ttatatttat tgcgcaccag aagatggaaa agactatgta gtttatttca ttacagatat
      721 taccagccag cgagaatttg ttcgtgggtt actggtagaa ggacgattgg tttggagtca
      781 gattggagga acggatgaat aagatcgatt atttgagaat gattgcgaaa tgtttagaag
      841 atatccttac cgcgattcat caaattctga agaaaaaagg agaaagaata tgaaatattt
      901 tcaactagta ttgacactta taacggcaat ggtaattgaa gtgcttgaac aaaaaatagc
      961 ataggagaaa aagagatgat tgaaaaagaa ttagtgacca cacaaacaga agtttggcga
     1021 gatcctcatc atacccatcg ctatgttttc aaacgacaat gggcgatcaa aggaaaagaa
     1081 gcggaagata aattagcagt agtcattact attcgtccaa cagatacaga accatttacg
     1141 aatgatttga gtatgttatt aatcgaaaag aatatccgac aattaggatt tactggtttt
     1201 attgctgtga atcttttttc gtacacaaaa gcgaaaagtc ctcgggtatt tcttcgagga
     1261 aatgatgaac aatctttgga aatattaacg actgttttta cagaaaagaa aatcaatacc
     1321 attatttttg cttgtggttc gattacagcg acgagtcaag tggcttatga acaagcaaag
     1381 actatttacg atcaattgag tgcgaaacaa aagaagatga ccaaagtatt gataaatgca
     1441 gaaggtaagt tatcccaccc actaagtatt catgttcgta atgaatggca attggcagat
     1501 agtcaattat tatttcaaga aaaataacat tttattttct catgttactt ataagtagtt
     1561 ttacatgctt tttgacttta aaaatacatg tgatgataca tgttatatga attatcaatg
     1621 tacccagtaa tatgctattt tcattatgta aaaaataaca gggggtgtgt agatggagat
     1681 tgatgaaact tgtttgagta actcttataa aagtatgaat gacttttttg aggaaatgga
     1741 actggattca gaattgctct atcaatttta tcttgctttt cgagggcaac agatttcttt
     1801 tccaatgaaa atgtacgatc gtgagttagt tagaaaaaga atcgaaaaca tgatagaaca
     1861 ggaaaaaact gtagatatcc gtcaattgac agaagtttat ggtttctctg caagatggat
     1921 tcaaaaagta attaaacaaa aagaaaggag acgagtacat ggctaagctg attgtattag
     1981 gtggtttaat ttacggtgga tattggttat ataaatatat gcttgcgcat ccgaatatag
     2041 ataaaggaaa agtaattaag acagttgggg taatcatagt tggactcata gctctaacta
     2101 agaaaaataa tactaatagc ccaaaaataa tgagaactta tcgctgtaga ggatgtggaa
     2161 gggaaatttc tactagtttt aatccatctg agagtcctca aggaagagac cgctgccata
     2221 cgggctatcg acattattgg gaaagaatcg attaaaagat taccatttag atcaattaat
     2281 tgtctgatag aataagtacg tgagggagaa attgaaaggg ggaaatataa tgaaaaaatg
     2341 gctgttatta ctaataggaa tgggaatttt attcactggt tgtacatctg agaatacgag
     2401 gactaaagcg actatagaaa ctgatacaac aattgagact aaaaaaagta aaacgcaatc
     2461 aactgaacaa actacaacag aaatgaagca atctgtggaa acaagtgcaa gttcaatcac
     2521 tgaatcttct tctgaagagg ttaagaatac tttgtgggat acgaataaag cttccaaatt
     2581 agaaactttt gtcacacaat ggggaaagac attaggtcaa gaatataaaa gctacaatct
     2641 tcagaataat gtaagtctgt atggcactcc tttaccacaa gccgtgatta atggcgattg
     2701 gaaaatggca atcaatgaag caccagttac cgtacaatgg tcagaagatg gaactggtca
     2761 tgcagatttc aatttagtag cagtttattc tgatgtagaa actggagaat atttagggca
     2821 gcatgtttac tttttcggtt ttcaaaatgg acaacctaaa gtttatttaa cgcaacaaaa
     2881 tcaaggtaat gaaaataatt atctgtactt taacgaaact caaaatcaac agttaaaaca
     2941 aggtttcagt gatattgtga atggacaaac tcctcaaaca ccagtagtag aagaatctac
     3001 tcaacaagca acaactacaa cgacacaatc tacaagtagt tcaggaattt caagttggga
     3061 agctgctaaa gaatctgtgc taaataataa agctttatgg ttgtatgatt ctcctgataa
     3121 aaatgtatca aatactaatt taattagcac aaatgaatat atgaaagatc taatgcaaga
     3181 tgaaaatgga agctactatt caattaatat tgtaacggat gatgatggaa acacgaccat
     3241 ggctggtgga aaacaatttc gtgtttatac agatggaaga atttctcaac gtattggaat
     3301 gactaacaat tttcaaatga ttaaataatt gaaaatatta tttatcaaag aaaaactgat
     3361 tttgagaaaa attatttaat tttcttagaa tc
//
LOCUS       DACEPD010000157         3369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.166, whole genome shotgun sequence.
ACCESSION   DACEPD010000157 DACEPD010000000
VERSION     DACEPD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.166"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IW797_002699"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IW797_002699"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ1225444.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IW797_002700"
     CDS             complement(2260..2511)
                     /locus_tag="IW797_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ1225445.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IW797_002701"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IW797_002701"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ1225446.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          920 a          739 c          626 g         1084 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaa
//
LOCUS       DACEPD010000158         3230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.167, whole genome shotgun sequence.
ACCESSION   DACEPD010000158 DACEPD010000000
VERSION     DACEPD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.167"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            183..1137
                     /locus_tag="IW797_002702"
                     /pseudo
     CDS             183..1137
                     /locus_tag="IW797_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 105 protein"
     gene            1154..2178
                     /locus_tag="IW797_002703"
                     /pseudo
     CDS             1154..2178
                     /locus_tag="IW797_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            2184..2663
                     /locus_tag="IW797_002704"
     CDS             2184..2663
                     /locus_tag="IW797_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ1225447.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            2679..>3230
                     /locus_tag="IW797_002705"
     CDS             2679..>3230
                     /locus_tag="IW797_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ1225448.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVG"
BASE COUNT          979 a          539 c          750 g          962 t
ORIGIN      
        1 cttggaaaac gttgtttctc caagttcttt ttttaatttc caagtaactg aaagttactt
       61 ttgttattta acatttaggt caattttaga cagtttttgt tcaaaaagcg atatttatag
      121 tggacggtat tactgatata ttaaggatat aaaatgaaag cgtttaaaaa ggtggcgtaa
      181 agatgattat taaagaagag atttttgtac ctaaagaatt agtgaaagaa aaaattgacc
      241 tgctcgttga aaacctgaca acgatcaaag acgataatgg tgaatttttg ttgggctttg
      301 atggactgaa agtagatgac aagagctgga cagtgtggaa ttggccacaa ggtgtcggtc
      361 tatatggtat ttataaaaat taccagatga caaaaaatcc tcgcgcttat caagtggtca
      421 atgaatggtt tgaggatcgt atggaagaag gcgctccgcc caaaaacgtc aatacaatgg
      481 caccgctatt gactatggct tacttgtatg aagacacgaa agattcacgc tatttgcctt
      541 atttagaaca atgggcagaa tgggtcatga atgacatgcc tcggacaaat gaagacgggt
      601 tacagcatgc cacatatgga ccggaaaata agaatcagtt gtgggacgat acattgatga
      661 tgacagtgct tcctttggca aaaatcggga aactgctggt tgacgattgc tattccagag
      721 atcattgaaa tcttagaatt atcgaaagaa gatgcattgc gcaaattatt gatcgaaaca
      781 cttgaagcac aagtgcgtgc attaaaaact tatcagtctg aaagcggttt atggcatacg
      841 ttgttggatg atccttcttc ttacgtagaa tcttctgcaa ctgcaggatt tgcttacggt
      901 attttgaaag cggtccataa acgttattta ccacaagaat ataaagaagt cgcttatcgt
      961 gctatccaag gattgcttga acaaatcgat gaaaaaggag aagttcaaaa cgtttcaatc
     1021 gggacaggaa tgggggacag tttagatttt tatcggaata tcggaatcac agcaatgcct
     1081 tatggacagt cgttaacggt gctttgtttg acagaattgc tcgtctctta ttgttaaaag
     1141 aaaggaaacc catatgaaaa aaacatcact gctgtttgca gcaagttcgc ttagcctagg
     1201 tctgctaggt ctaagcggct gcggaaataa ttctagtgtg gcaactgtcg cagatgcaga
     1261 cgtaaatgag atagttacct tgcgctttgc ttatgcaagc aacagccagt aattgatgca
     1321 atgaatgaat ttgggcgttt agtaaaagaa aaaacagatg gttccgtaca gatccagtat
     1381 ttcccagatg gacaactggg tggagaacgt gaactgatcg aattgacaca gtcaggagca
     1441 gtagatttca caaaagtaag tgcctcagcg ttggaaagtt tctctaaaga ttattcgatt
     1501 ttttccgttc cttatctatt caatgatgaa gaacatttct ttaaggtgat ggataatgaa
     1561 gaaataatgg agccaatcta ccaatcgacc aaaaatttag gatttaccgg attgacctat
     1621 tatgattctg gtcagctcag cttttatatg gtagacggtc cgatccatac gccggaagat
     1681 ttgaagggaa agaagatccg tgtgatgcaa agcgaaaccg ctatcaaaat ggtagagctt
     1741 ttaggcggtt cgcccgtacc tatgggaagt gatgaagtgt acacgtctct tcaatccaat
     1801 ttgatcaacg gatcagaaaa caatgaattt gtcttgtata cagctggaca tggcggggta
     1861 gctaaatact attcctatga tgagcataca cgtgtgccag atatcatcat catgaatgat
     1921 gccatcaaag aacagctaac tgatgaacag gaaaaagcca ttgaagaagc agcgaaagaa
     1981 tcaacggttt ttgaaattga agcttttgca aaagcaattg aagaagaaaa agctttagca
     2041 caagaagaac atggagtcca attcaatgat gtagataata caccgtttcg agaggcagtt
     2101 gccccacttc atgatatatt caaaaacgat cctacctaca aagaccttta tcaaaagatc
     2161 caagagcaag gagcgtagct gtaatgaaaa tgattttaaa caaggtatta gagatcctgg
     2221 gagctgtttt gcttgttggg atggtcgctg ttgttttatg gcaagtattt tccagaacgg
     2281 ttttaggaaa tccaaatacg gtcacggaag agcttgttcg attcggtctt gtctggtttg
     2341 ccatgttgtc ttccgcgtat gttgtgggac aaaaagcacg tttagccgta acggttttaa
     2401 gtgaaaaatt aactggcaaa aaaagtaaaa ttttagaagt tgttgttcaa agtttatttc
     2461 tggtatttgc tgcgatgatc atggtgtatg gcggatggaa tgctgtcaca ttgacaatgg
     2521 ggcagatctc gccttcttta agtattccta tgggatatgt ttatctgtca gtacctgtat
     2581 cgggggtact catcattatc tatagcctga tcaatctaat cgatacatta agcaacaagc
     2641 cagcaattca agaaaaaata taaaaatgga ggaataaaat ggcaatagaa gccggattag
     2701 tgttattttt tgtatttgtt gtgttgcttg tattagggat gccgattgct ataagtattg
     2761 cattctcatc gatgatgact ttattgttag ttataccgtt tgatgtttct gcttttagtt
     2821 cagctcaaaa aatggtggga agtatcaata gtttttcgct gatcgctatt ccgtttttcg
     2881 ttctctcggg cattatcatg aataatggag ggatagctaa gaaactagtg gattttgcga
     2941 tgttgttcgt cgggcgcgtg ccgggggctt tggctcatac gaatgtgtta gggaatgctt
     3001 tatttggttc catctctagc tcggccattg ccgcttctac cgcgattggt ggagtgttga
     3061 tccctcagca agtggaagaa ggatatgacc ggaagtttgc gacagcggtc aatatcgcgt
     3121 cagctccgac aggtatggtc attcctccaa gtactgcttt tatcatgtat tcacttgttg
     3181 ctggtggtgc ttcgatctct gctttgttta tgggaggtta tctcgtaggc
//
LOCUS       DACEPD010000159         3227 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.168, whole genome shotgun sequence.
ACCESSION   DACEPD010000159 DACEPD010000000
VERSION     DACEPD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.168"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(550..2163)
                     /locus_tag="IW797_002706"
     CDS             complement(550..2163)
                     /locus_tag="IW797_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ1225449.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2304..3212)
                     /locus_tag="IW797_002707"
     CDS             complement(2304..3212)
                     /locus_tag="IW797_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1225450.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1038 a          556 c          571 g         1062 t
ORIGIN      
        1 aaataaatag gaacaaaaaa attaaattat ttaatacaca cattagggat cattcattga
       61 aggattagaa gacaacgagg atggcgaaaa atatgattac aatagaaaag tcgttctaaa
      121 ccaatcagtt cctagtatca cacaacgcaa gccagtcgat ttggtgagat tagaggagtg
      181 aaagcaaact ttattatata agaaaaaata ttcgaaaggg atgctagttt aggtactagt
      241 gctgatagat taatatgatg cctcgtaata ttaggcgtag ttactctaga gacatcacta
      301 tggactatga tttgtctcag agtcacaaca tgttttttta tgaaaagaaa cagctaaaat
      361 aattaaattg cgcaatgttt aaactttctt aaactataac ctaataaatg tgaagaaata
      421 atgcacaatt tcttcacatt tatcttctat tctatataca taccaaccaa caaccctata
      481 tactttttca tttttactcc tcaagtaaaa ataactccta gaccgcggct acccaccgcg
      541 gtcgtttttt tattcttcaa tcaaaatcgc tttgataccc aaacctgcca agattcgggc
      601 agaagtataa gtattcaatc ctcttctgaa agtaatgtaa acttttttat cttttgggat
      661 ttcattgatt cgatctcgta actcattcat tggaatattt tttgttgccg agatactgcc
      721 agaaggtgct ttattaggtt cacgtatatc taaaaagaac gcttctttca aatcgttttc
      781 gggtatatca cttgctttga tcgtttctac gatattcgtc atttgattga ttgcgacata
      841 accagctata ttcaacgggt cacgggtagt agaatacggt ggagagtaag gcaactccaa
      901 atcaggcaaa tcaaagactg tcaagtttcc ggtgattgct acagaaagtt ctcccatccg
      961 tttatcgaca cctttttctc caaatgcttg tccacctaaa atacgaccgc tatctgcatc
     1021 aaaaatcagc tttagattta atcttgtagc ttctggatag aaataggcat ggtcaaaggg
     1081 agtgataaaa acagttttat aatttgttat gcctgatgct tttaattcat gttctgtcat
     1141 tcccgcataa cttgctgtat gatcaaagat ttttgcgaca cctgcaccaa tatatccgcg
     1201 atagcgcata ggcgtaccat ttaaaatatc tgccagcata tgtccttgac gattggccgc
     1261 actggaaagc atactttgga tcggctgacc agtcactaca ctagtcgttt cgataatatc
     1321 gccaatcgca tagatgtccg gcagattggt ttgtaactgg tcatcaacaa tgatctgacc
     1381 tgtatctgat aattgtatgc cggttgcttt caccacttca ttcttcggag aaacaccaac
     1441 agcaaaaatt agcatatcag cagaaaggac ggagccatct gataatacta tttctcgacc
     1501 tttatcacgg atctcagtaa ctcttgtttc taaatgaacg gctaatcctt ctttcagcaa
     1561 tttgtcataa actaaattag caatttctgg atcataaggg aaagcgactt gtggcaattg
     1621 atcgattaaa gtcacgtcta agccgcgatg cttgaaattc tcagctaatt ccagacccat
     1681 aacacctgca ccaagaatga cgacatgttg tggatttttt tcttcaagga acgatttgat
     1741 tcgatccgca tccgtcacac tacggaggac aaagccgttt tctgcttcaa aaactccggg
     1801 aatatccgga taatctggtc tagcaccaga agagatgatc aacttatcgt aatctgtttt
     1861 tatttcttca tttgtggata ggtctttaac tgttaatcgc ttagtagaag gatcgattgc
     1921 tgttacttcg tgtttagtaa agacatcgat gttgtttttt gttttcaata tttctggtgt
     1981 acgttcgatc agcgagtcac ggtcagtgat cacacctcct aaataataag gcaaagcaca
     2041 acttgcataa gaaatatttt ctccgcgttc atagataatg atttcgtgcg cttcattcaa
     2101 tcgacggaaa cgagtggcaa atgaaggacc gccagctaca ccgccaatga caacaacttt
     2161 cataaaaaca tctccttttc tacgttgatc gtactatgtt atggtaaaaa acagcaaata
     2221 attgtgccgg aaaattattg tttattttga atagacagaa aatatagaga aataatttgt
     2281 ggataaaacc taaaaatttt tttctatttt aaggatagat aatactttga aggggataac
     2341 cctgtatatt ttttaaaagc tgaagaaaaa tgatggatcg aatgaaatcc taatatatct
     2401 gcaatttctg tataggttaa ttcattttct agcacgagac gttttgcttt ttccattttt
     2461 agttctttaa aatagctaat aacacttttt ccattcactt ttctaaaatg tttttttaag
     2521 ctggctggac tgataaaaaa aatttgggct aattgttcaa gcaaaaaatt ttctgaaaga
     2581 tgttcttcta aatatcgtat gattggatca actaattcat ctgaataaag aatatgcttt
     2641 tttttaggaa tgactttctc atttgaaggt attgtcaata aagcaagttc ctgttgataa
     2701 tgccttggta aggaagacaa ttgaattttt tgcagacaat aagtaactaa taaggaaagc
     2761 aaatgaatgc tcatctgttg gcatagtatg aaaaaagaat tgatttcctg atcataggaa
     2821 atagctggct tactatgttt tagataatca attttgaact gttcattttt cagaaaagta
     2881 atgatagatg ttatttcttg atctatagaa aaagattgtc ccgtaatatg tgctaataat
     2941 tgctcatcaa aataaaactg gataatgata aaagtttgtg tttccttttg agtagaaaag
     3001 atttggactt cttgctgttt atcaatgaat aaaagggaag gattatctag ttctatagat
     3061 ttatgtgcaa attcagcata taaattagcg ctttcattta ttagtagtcg aaaataggga
     3121 gcagcagata aatgaagatg ggtatctatg gaataggtta gtgttttttt gacaagattg
     3181 gaggattggt tgtttgttaa caaaagaacc actttccttt ctctttt
//
LOCUS       DACEPD010000160         3220 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.169, whole genome shotgun sequence.
ACCESSION   DACEPD010000160 DACEPD010000000
VERSION     DACEPD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.169"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            50..1438
                     /locus_tag="IW797_002708"
     CDS             50..1438
                     /locus_tag="IW797_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAQ1225451.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>3220
                     /locus_tag="IW797_002709"
     CDS             1776..>3220
                     /locus_tag="IW797_002709"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ1225452.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1184 a          505 c          583 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaagaaga
//
LOCUS       DACEPD010000161         3208 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.170, whole genome shotgun sequence.
ACCESSION   DACEPD010000161 DACEPD010000000
VERSION     DACEPD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.170"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            115..975
                     /locus_tag="IW797_002710"
                     /pseudo
     CDS             115..975
                     /locus_tag="IW797_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
     gene            987..1814
                     /gene="panB"
                     /locus_tag="IW797_002711"
     CDS             987..1814
                     /gene="panB"
                     /locus_tag="IW797_002711"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAQ1225453.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1829..2677
                     /locus_tag="IW797_002712"
     CDS             1829..2677
                     /locus_tag="IW797_002712"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAQ1225454.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2687..3082
                     /locus_tag="IW797_002713"
     CDS             2687..3082
                     /locus_tag="IW797_002713"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAQ1225455.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
BASE COUNT         1246 a          326 c          641 g          995 t
ORIGIN      
        1 atattatgat aaattaacta tggatgatat ttgaaaaaaa catactgtat atttgattta
       61 atttttattt ttaagctcat atcgtaagaa tgggacttgt agtctcactt ctttatggag
      121 gtgataatta tgaacgttgg ttttattgga gccggcaagg taggttgttc attcgggaag
      181 tatttccagg aacataaaat acaagtaaca ggattttata gtaaaagcga agactcgtca
      241 ttagcagctt cgaattttac atctagtaaa caatatttaa acttaagaga attagtagat
      301 gaaaatgata ctattttcat tacgactcct gatggtcaaa ttcaggaggt atggcaagaa
      361 attaaaaatt attaaatcaa aaacaaatta atctgccatt gcagtggtgc aatatcttca
      421 gatatctttt caaatataca ggactatggt gcttatggct attctgtcca tcctatgttt
      481 tcaatatcag ataaatataa ttcatacaaa aaattaaaaa aatcttttat aacaattgaa
      541 ggcgatgaaa aatatgcaaa gtttttatgt gatttcttca aaaagttagg taatagtact
      601 gtaattgtat ctaaggagaa taaatctctt tatcatgccg catcagtagt atctagtaac
      661 cttgtgctag gacttattaa tacaagcgtt acatatctaa ttcaatgtgg atttactgaa
      721 aaaatggcaa ttgaagcttt atatccatta attgaattta atattagaaa tataaaagaa
      781 aaaggaatta tagaaagttt gacagggcca gttgaacgtt gtgatatttc aacaattaaa
      841 ggtcactgtg aagttttatc agatgaagac agagctttat atatgctatt atcaaaaaat
      901 gtattagaga tagctatgtt aaaaaatatt aatagagatt atagtgaatt agaaaagtat
      961 ttaggagaga gttaggagag aaatagatga aaaatacagc agttacattt aaagaatcta
     1021 aattaagaaa tgaaaaatta acaatgctta cagcttatga ctattcaaca gcaaaaataa
     1081 ttgatgaagc tgggataaat ggaatattag taggagattc tcttggtatg gtatgtctag
     1141 gacatgaaga tacactttca gtaacaatgg aagacatgat tcatcatact agagcagtta
     1201 caagaggagc taagaacact ttagttgttg cagatatgcc atttatgtct tatcaaactt
     1261 cagtatatga ttcagtagtt aatgcaggaa gattaataaa agaaggacga gctcaggttg
     1321 ttaaattaga aggagggata gaagtatgcg ataaaataga ggctatagta aaggcttcta
     1381 tacctgttat ggcgcatata ggtttaacac ctcaatctgt taatgcattt ggaggattta
     1441 aggtacaagg aaaggacaag gaagctgcta aagaattaat aagagctgct aaagctgtag
     1501 aaaaagcagg agcattcgct gtagtgttag aatgtgttcc aactaagctt gcagagttaa
     1561 tttctaaaga gatatcaata cctacaattg gtattggagc aggtgcaggg tgtgatggac
     1621 aaattcttgt atatcaagat atgttgggga tgtatagtga ttttacccct aaatttgtga
     1681 agaaatatgc taatttatca gaagagatga ataaagcatt tacaaaatat atagaggaag
     1741 taaaagatgg agtttttcca ggtccagaac atgggtttaa aataagtgat gatgttttag
     1801 aaaaattata ttaaaagggt caagaaatat ggttatattt aaaacagtga atgatgttag
     1861 aagtcaagta aaagagtgga aaaaacaagg cttaaaggta ggattagttc ctactatggg
     1921 atatttacat gaaggtcatg aaagtttaat tagaaaagca tcaaaagaaa atgataaagt
     1981 tgttgtaagt atctttgtaa atccaactca gtttgggaaa aatgaagatt taggtagtta
     2041 tccaagagac ttagaaagag atatagaagt atgcacaaga ggtagagcaa cagcaatatt
     2101 taatcctgaa gttgaagaga tgtattgtga caatgcaagt acttttgtta atataactgg
     2161 attaacagaa ggtttatgtg gagctagtag gccaatacat tttagagggg tttgcactgt
     2221 tgtaagtaaa ttatttaaca taataccagc agatagagct tattttggag aaaaggatgc
     2281 acaacagtta gctgtaatta aaagaatggt tagggacctt aatattgata tagacgttgt
     2341 agggtgtcca attataaggg aagaggatgg actagctaaa agttctagaa acacatactt
     2401 aagtttagag gaaagatctt cagctactat tttaaataaa tctttaactt tagctaagga
     2461 agctttaaat aatggagaga gagacagctt aaaaataatt gagataataa gtaaaaatat
     2521 aaatacttat aatttggcaa agattgatta tgtagaagtt gttgattctt tatcattaca
     2581 aagagtaaat tatattgaaa aatcagtttt agttgcaata gcagtattta taggaaaaac
     2641 aagattaata gataatttta catttgaatt acaatagggg gctataatga aagttacaat
     2701 gttaaaagga aaaatacata gagctacagt tgaacaagct gagttagatt atgtaggaag
     2761 tattactgtt gatatgaatt tattagaagc agcaggaata tatgaatatg aaaaagtaca
     2821 aatagttgat gttaataatg gaaatagatt tgaaacttat acaatagctg gagagagagg
     2881 aacagggatg atttgcttga atggagctgc agctagatgt gtatcaactg gagataaaat
     2941 aattttaatg gcatattgtg aattaaatga aaatgaggtt aaggatcata gacctaaggt
     3001 tgtttttgtc gatgataata ataaagttga aagagtaaca tcatatgaaa aacatggaag
     3061 gttatcagat ataattcttt aggttttgta atgttattta tatatagcta gactattatt
     3121 gaaatattta ttgattacta attggataga tgtcaataaa gtagacacaa aaagagaaga
     3181 tacagactgt agacaaagtc cattttat
//
LOCUS       DACEPD010000162         3168 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.171, whole genome shotgun sequence.
ACCESSION   DACEPD010000162 DACEPD010000000
VERSION     DACEPD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.171"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(862..1203)
                     /locus_tag="IW797_002714"
     CDS             complement(862..1203)
                     /locus_tag="IW797_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ1225456.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1203..1433)
                     /locus_tag="IW797_002715"
     CDS             complement(1203..1433)
                     /locus_tag="IW797_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1225457.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1720..2058
                     /locus_tag="IW797_002716"
     CDS             1720..2058
                     /locus_tag="IW797_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225458.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2263..2502)
                     /locus_tag="IW797_002717"
     CDS             complement(2263..2502)
                     /locus_tag="IW797_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ1225459.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2478..2825)
                     /locus_tag="IW797_002718"
     CDS             complement(2478..2825)
                     /locus_tag="IW797_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ1225460.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         1004 a          578 c          489 g         1097 t
ORIGIN      
        1 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
       61 aataatattg acaaaaagtg actcaaaata aaaaaaaact tacattatac tggaatgaac
      121 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
      181 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
      241 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
      301 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
      361 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
      421 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
      481 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
      541 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
      601 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
      661 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
      721 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
      781 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
      841 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
      901 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
      961 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
     1021 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
     1081 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
     1141 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
     1201 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
     1261 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
     1321 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
     1381 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
     1441 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
     1501 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
     1561 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
     1621 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
     1681 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
     1741 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
     1801 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
     1861 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
     1921 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
     1981 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
     2041 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
     2101 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
     2161 tatatgcagc ttatgagtat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
     2221 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
     2281 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
     2341 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
     2401 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
     2461 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
     2521 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
     2581 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
     2641 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
     2701 agatttgacg gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc
     2761 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
     2821 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
     2881 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
     2941 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
     3001 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
     3061 gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
     3121 gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgact
//
LOCUS       DACEPD010000163         3008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.172, whole genome shotgun sequence.
ACCESSION   DACEPD010000163 DACEPD010000000
VERSION     DACEPD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.172"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            441..725
                     /locus_tag="IW797_002719"
     CDS             441..725
                     /locus_tag="IW797_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225461.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            728..1144
                     /locus_tag="IW797_002720"
     CDS             728..1144
                     /locus_tag="IW797_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225462.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1281..2000)
                     /locus_tag="IW797_002721"
     CDS             complement(1281..2000)
                     /locus_tag="IW797_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ1225463.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2653..2748
                     /locus_tag="IW797_002722"
     CDS             2653..2748
                     /locus_tag="IW797_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ1225464.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
BASE COUNT         1008 a          565 c          478 g          957 t
ORIGIN      
        1 aaaaaaactc ttagaagcga ttttagagcc atttaaaccc acttaattat attctccatg
       61 aaattcgctc catgcgtcaa aagctgcgtc tgaaattccg ccgtcttcgt tcactagttg
      121 cattaatttt tccaacgatc gttttctttc gggtgtcata tttttatatt ccaacaattt
      181 ttcgtctgtt aaatttggtt atcgattaca cgtgcagcac tcccccccga attttaaaga
      241 gcataaggaa acgtgtaccc actttctaaa aaaagtgggt agataggctg tgcctatcac
      301 gtttctcaga aaaaaatttg atatcacttt ttcgccatcg tgggtagctt atgtatagca
      361 ttttggcaaa aaacagctat acaaatatag tatttgtatg gtatacataa attatacata
      421 attgcaaaag gagatttttt atggacattt tagttcggaa tattgatcca aagtttgttg
      481 caacaataga caaacgttgc caagaactca cgaataaaac aggagaaaaa tggagtcgaa
      541 ataaatatct aaaagtttta atcgaaagtg attttgaccg tcaacttacg acttacaaaa
      601 aagaccaatt cgatttattg ctcgataaat ttattgctat ccaaacagaa aatactcaaa
      661 tattaagcga gtatgtctac acaaataatc aaatcattga aatgcttatc ggaggaatga
      721 tataaccatg agaaaaactt ctgtccaaaa acatgttact ttgtacccta gtcatgttga
      781 cgttttagat tccatcatcg aaaaaaatcc agatatcaat aactactcgg aagccattcg
      841 ctttttatgt atgagttatg aacccgatac caacacgacc actaccagca atcaggcaaa
      901 actaaatgct atgggaaaag aaatttccat catgactgaa atcatcgcag aatttgctga
      961 tttatatcta aaagatacag gcgttcttat tggcacaaat tctaaagtat atcaagaagt
     1021 gaaagaaaaa atcgaagcta aactaaatcc atcaaatcga aatcgaccaa agaatcatca
     1081 aaaagaaacc agtcaaacag tcacttctag aaatttcgac ccaacaaatt ttctaaaacg
     1141 ataaaaaaca gataggagtt attcctatct gttttttatt tagaagcccc atacaatatg
     1201 ctagaatgta ctagcaatct tagtcggttg taagggactt gtctacttgc ttaatgtaag
     1261 gggggcgagt tcttttttat ttattcttct aaccagttac gcagagaaat ctttggcaaa
     1321 tctgacgtta ggcttgaaaa agtaaattct aatttggcga ttttattgcc cttttttgct
     1381 tttatttttg tgactttaag atcattgaag tattttttta gttctcgtaa tgcaggattt
     1441 aatacttttt gatcaatgtt acccatttga tagtattctg ggatatccat taagctccta
     1501 aactcttcaa tacgaactac ataataacca gtagaacggt actgcataag cagtcggaat
     1561 agagtttttg catacgtaga acgcaacgac gtgaaagcag ataattcaaa tctactaaat
     1621 tcagctgtta aaccattaat aatatattct aggtccggat taacactgac ttcaacaaac
     1681 ttttttgttt tattgatttc aaaaccagta aacaaaacaa agtagcgaat agaatcctca
     1741 tcttcagtac ggtaagtaag attaagcata tctttataga gcttttctaa atcaccgaca
     1801 aaggcttttg tagcagtcat cttataatca cttaactctt ttaattcctc gaaagaaaat
     1861 ctaacttttt tcaaaccacg atctttcata cgagcgcata atgcaaaaaa taaatccatc
     1921 tgcttagcat tgaaattttt cagcggtact aaattcaatt cattttgata tttgactgtt
     1981 aattccgttt tttcatccat tcctttcttc cccccttaca aataaataat aacctttaac
     2041 aaccaactcg taaataggtt ggttgttatt atacaaaagg atattagtac tcagataaaa
     2101 agttgttagt actcagataa aaagttgtta gtactcagat aaaaagttgt tagtactcag
     2161 ataaaaagtt gttagtaccc ttctgtatcc gttgggagag taaggctcaa ctgcccccta
     2221 aaaagggttc taaaaaatct ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa
     2281 tttcccgcca aaaaatcacc cactttttaa aataaacagg ttcgcgcatg atattaaact
     2341 gccaatctag ctccctatgg tcgctgtaag aaggagtgat cgctacgctc tcacatttta
     2401 cttgcagcag tcgcctttag gctccttcgc caaaaataat tcattaggga ctcctttgtg
     2461 gcgtcctgag agctcgtcta agccgttcta agacaagatt cgataaaaac tatgtccttc
     2521 gttctaaagc gccttacagg cggtcagggc aagccctaac aaccccaagg caataaaaaa
     2581 cttaggctta tttttgcttt tttattgtat aattgattgt cgcctatccg acagtcttgc
     2641 gactggaagg aggtgtgttt cgtgttcact aactttattt gctcccttct tgttgggctt
     2701 ttaagcgctt tgttcagtta ctggttgaat aaccgaaaca agaagtaaaa cttaattaag
     2761 gcgactcagc ccacaaaaaa agccccagag aatccgacct ctctggggat ttcgtgtgtt
     2821 tcgtgttcac ttatttgcaa tcaaattata acagaaataa atatttattt caaaaatatc
     2881 ctacaaaagt ctaaaatccg taattatttc ttatatgcaa catttacaaa taaagtcgct
     2941 ctgagagctc gtatttttcg atttaagacg ttttatttag tttttggtgt atttcattgg
     3001 tccaagct
//
LOCUS       DACEPD010000164         3003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.173, whole genome shotgun sequence.
ACCESSION   DACEPD010000164 DACEPD010000000
VERSION     DACEPD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.173"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(106..1053)
                     /locus_tag="IW797_002723"
     CDS             complement(106..1053)
                     /locus_tag="IW797_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ1225465.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IW797_002724"
     CDS             1299..1763
                     /locus_tag="IW797_002724"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ1225466.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IW797_002725"
     CDS             complement(1825..2868)
                     /locus_tag="IW797_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ1225467.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          992 a          596 c          574 g          841 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     3001 tcc
//
LOCUS       DACEPD010000165         2960 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.175, whole genome shotgun sequence.
ACCESSION   DACEPD010000165 DACEPD010000000
VERSION     DACEPD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.175"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="IW797_002726"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="IW797_002726"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ1225468.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="IW797_002727"
     CDS             complement(1034..1954)
                     /locus_tag="IW797_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ1225469.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /gene="xth"
                     /locus_tag="IW797_002728"
     CDS             2067..2819
                     /gene="xth"
                     /locus_tag="IW797_002728"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ1225470.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          831 a          596 c          513 g         1020 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt
//
LOCUS       DACEPD010000166         2918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.176, whole genome shotgun sequence.
ACCESSION   DACEPD010000166 DACEPD010000000
VERSION     DACEPD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.176"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            205..1173
                     /locus_tag="IW797_002729"
     CDS             205..1173
                     /locus_tag="IW797_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1225471.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1166..2197
                     /gene="vanA"
                     /locus_tag="IW797_002730"
     CDS             1166..2197
                     /gene="vanA"
                     /locus_tag="IW797_002730"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1225472.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2203..2811
                     /locus_tag="IW797_002731"
     CDS             2203..2811
                     /locus_tag="IW797_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1225473.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          863 a          559 c          708 g          788 t
ORIGIN      
        1 taatttttta ggaaaatctc aaggttatct ttactttttc ttaggaaatt aacaatttaa
       61 tattaagaaa cggctcgttc ttacacggta gacttaatac cgtaagaacg agccgttttc
      121 gttcttcaga gaaagatttg acaagattac cattggcatc cccgttttat ttggtgcctt
      181 tcacagaaag ggttggtctt aattatgaat aacatcggca ttactgttta tggatgtgag
      241 caggatgagg cagatgcatt ccatgctctt tcgcctcgct ttggcgttat ggcaacgata
      301 attaacgcca acgtgtcgga atccaacgcc aaatccgcgc ctttcaatca atgtatcagt
      361 gtgggacata aatcagagat ttccgcctct attcttcttg cgctgaagag agccggtgtg
      421 aaatatattt ctacccgaag catcggctgc aatcatatag atacaactgc tgctaagaga
      481 atgggcatca ctgtcgacaa tgtggcgtac tcgccggata gcgttgccga ttatactatg
      541 atgctaattc ttatggcagt acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat
      601 gatttcaggt tggacagcga ccgtggcaag gtactcagcg acatgacagt tggtgtggtg
      661 ggaacgggcc agataggcaa agcggttatt gagcggctgc gaggatttgg atgtaaagtg
      721 ttggcttata gtcgcagccg aagtatagag gtaaactatg taccgtttga tgagttgctg
      781 caaaatagcg atatcgttac gcttcatgtg ccgctcaata cggatacgca ctatattatc
      841 agccacgaac aaatacagag aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt
      901 ccacttgtag atacctatga gttggttaaa gcattagaaa acgggaaact gggcggtgcc
      961 gcattggatg tattggaagg agaggaagag tttttctact ctgattgcac ccaaaaacca
     1021 attgataatc aatttttact taaacttcaa agaatgccta acgtgataat cacaccgcat
     1081 acggcctatt ataccgagca agcgttgcgt gataccgttg aaaaaaccat taaaaactgt
     1141 ttggattttg aaaggagaca ggagcatgaa tagaataaaa gttgcaatac tgtttggggg
     1201 ttgctcagag gagcatgacg tatcggtaaa atctgcaata gagatagccg ctaacattaa
     1261 taaagaaaaa tacgagccgt tatacattgg aattacgaaa tctggtgtat ggaaaatgtg
     1321 cgaaaaacct tgcgcggaat gggaaaacga caattgctat tcagctgtac tctcgccgga
     1381 taaaaaaatg cacggattac ttgttaaaaa gaaccatgaa tatgaaatca accatgttga
     1441 tgtagcattt tcagctttgc atggcaagtc aggtgaagat ggatccatac aaggtctgtt
     1501 tgaattgtcc ggtatccctt ttgtaggctg cgatattcaa agctcagcaa tttgtatgga
     1561 caaatcgttg acatacatcg ttgcgaaaaa tgctgggata gctactcccg ccttttgggt
     1621 tattaataaa gatgataggc cggtggcagc tacgtttacc tatcctgttt ttgttaagcc
     1681 ggcgcgttca ggctcatcct tcggtgtgaa aaaagtcaat agcgcggacg aattggacta
     1741 cgcaattgaa tcggcaagac aatatgacag caaaatctta attgagcagg ctgtttcggg
     1801 ctgtgaggtc ggttgtgcgg tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga
     1861 ccaaatcagg ctgcagtacg gaatctttcg tattcatcag gaagtcgagc cggaaaaagg
     1921 ctctgaaaac gcagttataa ccgttcccgc agacctttca gcagaggagc gaggacggat
     1981 acaggaaacg gcaaaaaaaa tatataaagc gctcggctgt agaggtctag cccgtgtgga
     2041 tatgttttta caagataacg gccgcattgt actgaacgaa gtcaatactc tgcccggttt
     2101 cacgtcatac agtcgttatc cccgtatgat ggccgctgca ggtattgcac ttcccgaact
     2161 gattgaccgc ttgatcgtat tagcgttaaa ggggtgataa gcatggaaat aggatttact
     2221 tttttagatg aaatagtaca cggtgttcgt tgggacgcta aatatgccac ttgggataat
     2281 ttcaccggaa aaccggttga cggttatgaa gtaaatcgca ttgtagggac atacgagttg
     2341 gctgaatcgc ttttgaaggc aaaagaactg gctgctaccc aagggtacgg attgcttcta
     2401 tgggacggtt accgtcctaa tcgtgctgta aactgtttta tgcaatgggc tgcacagccg
     2461 gaaaataacc tgacaaagga aagttattat cccaatattg accgaactga gatgatttca
     2521 aaaggatacg tggcttcaaa atcaagccat agccgcggca gtgccattga tcttacgctt
     2581 tatcgattag acacgggtga gcttgtacca atggggagcc gatttgattt tatggatgaa
     2641 cgctctcatc atgcggcaaa tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg
     2701 cgctccatca tggaaaacag tgggtttgaa gcatatagcc tcgaatggtg gcactatgta
     2761 ttaagagacg aaccataccc caatagctat tttgatttcc ccgttaaata aacttttaac
     2821 cgttgcacgg acaaactata taagctaact ctttcggcag gaaacccgac gtatgtaact
     2881 ggttcttagg gaatttatat atagtagata gtattgaa
//
LOCUS       DACEPD010000167         2862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.177, whole genome shotgun sequence.
ACCESSION   DACEPD010000167 DACEPD010000000
VERSION     DACEPD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.177"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            5..2377
                     /locus_tag="IW797_002732"
     CDS             5..2377
                     /locus_tag="IW797_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225474.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..>2862
                     /locus_tag="IW797_002733"
     CDS             2438..>2862
                     /locus_tag="IW797_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225475.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYA"
BASE COUNT         1144 a          455 c          621 g          642 t
ORIGIN      
        1 ggacatggca gaagaaaaag aaccactaga caatagtcgc ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaacga acaaattgag aagatgtttg aacaccagag
      121 aaagaccaac ggtcagccaa tgaacgataa gagaaatggg cattcttggt ttaggcaaca
      181 agaacgttta gaaaacaaag tgcatagtct gagagaagag atcaagcaac aagaaaaaca
      241 ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 cctggatatg acaatcgaaa atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg actttttcag ccgctacgat ccgaaaatac caacggaaat tggaaacatt
      421 agaacagctg aaagaaagat cagaaaaagg aaaagaaaat cttcttcctg aagtacaagc
      481 aattattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 agactatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctgc
      661 gccaactgat tctttgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tagaattgat
      781 cgatcaactc aaaacgttgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccca tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 aaaggaacaa aaacaaacgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaagcaaga
     1081 agtcaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtgaaa gaacttttga attttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ctgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actacacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgggg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cttcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 ggcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctgtagaaac ggcagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtga ttttaaaagg
     2221 aaaaagttat ggaaaagaga ttcaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg aaacagtgtc agaagtggcc aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg cgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg tttgaaaatg accgaaaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagact atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ctctaagcaa caatttaaag cagccacaga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtagaa aatcttcatt ttgagacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gc
//
LOCUS       DACEPD010000168         2784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.178, whole genome shotgun sequence.
ACCESSION   DACEPD010000168 DACEPD010000000
VERSION     DACEPD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.178"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1023
                     /locus_tag="IW797_002734"
     CDS             <1..1023
                     /locus_tag="IW797_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ1225476.1"
                     /translation="NYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTD
                     LNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVT
                     VTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNK
                     KGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLP
                     DGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNG
                     SKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKN
                     RKKENE"
     gene            1157..2500
                     /locus_tag="IW797_002735"
     CDS             1157..2500
                     /locus_tag="IW797_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ1225477.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2612..>2784
                     /locus_tag="IW797_002736"
     CDS             2612..>2784
                     /locus_tag="IW797_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1225478.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT         1111 a          361 c          516 g          796 t
ORIGIN      
        1 aattatgaag gtgtcattga agtaacgtat ccagacggta cgaaagatac ggtgaaagtt
       61 ccagtagaag tgacagacaa tcgttctaat gcagataaat acaatcctga aggacaaaaa
      121 gtgactacag acttaaataa agagccagat gcatctgagg gaattaagaa caaagaagat
      181 ttaccaaaag gaactactta tgcttggaaa gagaaagtag atgttagcac agctggaaat
      241 aaaaaaggta ctattgtagt gacatatcca gatggatcat ctgatgaagt tgaagtagac
      301 gttacagtga cagatactcg ttccgatgca gataaatata atcctgaagg acaaaaagtg
      361 actacagact taaataaaga gccagatgca tctgagggaa ttaagaacaa agaagatcta
      421 ccaaaaggaa ctacttatac ttggaaagaa aaagtagatg ttagtacagc tggaaacaaa
      481 aaaggtactg tagtagtaac atatccagat ggatctaaag aagaagtaga agttactatt
      541 tctgtagtag ataaaaaagc accgaataaa cctcaagttg atcctattac agagggtgac
      601 caaattgtta ctggtaaaac tgaaccaaat gcagaggtga cagttacatt accagacgga
      661 agtcaacacc atgggacagc tgataaaaat ggtaatttta cagttaaagt tcctaaatta
      721 gaggcaggga caaaagtgat agtaactgca actgatgaat ctggtaatac tagtgaacct
      781 actaatgtag ttgtttcttc caacaaaaaa gacagtggaa aaaacggtag taaaggtagt
      841 aagactgaca atcaaggtag caacagcaat caagacaaaa atagaggcaa aagccagtcc
      901 tcaaaagttt ttcctaagac gggagagagt gacagtaata tctttactat tagcggtggt
      961 ttaatactgt taggaacgtt aggattatta gggtataaaa atcgaaaaaa agagaatgaa
     1021 tagaaaatca atttcttaag tcttctctga gaagaggaag aatctttttc ttcctctttt
     1081 tgctttttta tgatgggaaa taagtgttag ttaaagatga atatactttt tatagaaagg
     1141 aaaagaatgt aattaagtgg aaaaaatagt aattattggt tcaaaccatt cagggctagc
     1201 cgcagctcga tttctaaact taagcgattc aaattatgaa gttattctaa tcgataaaaa
     1261 atcagaaatt agttacttgg aatgtgggac atcgttattt ataaaaggag aagtaagtaa
     1321 tttagaagat cttttttatc aaaatagaat gacactaaat tctgagataa aaaagttatt
     1381 tctagagaca gaagttgaca aaattgatat tcaaaataaa aagattagtt gtgtaaaaaa
     1441 agatggtaca agattctctc aagaatatga caaagttata ttagctacag gttcaaagca
     1501 aattatgcta gatataccgg ggaataattt agctaatatt ttttcgataa agaattttcc
     1561 aaatgcaaaa gagattttta aagctttgga tgatagtact attaaaaaag taggaattat
     1621 tggagcagga tatattggag ttgaaattgc tgaagccata agaaaaagag ggaaagaagt
     1681 atatcttttc gatgttgcag atagagtgtt gtcgacttat tacgaccgta gtttttcaga
     1741 taaagttgaa gaaatactat caaaaaatgg aatacatctt tgtctatcag aaagtcctaa
     1801 aaaatattta ggaagaaaaa agattgaaaa aatagtcact acaagtggta cccaatactc
     1861 tatggatatg gtcgtacaag ctataggatt tctatctaat agtccaattg gggaaaagga
     1921 actcttgaga gatgttagtg gagcatatct aactaatgaa taccagcaaa caaatataaa
     1981 agatatatat gctattgggg attgtgctac aacttttttt acgtccatta atagaagtag
     2041 tgttgatttt tctatttcta atgctctaag aacagcttat attgcaactc aacatattaa
     2101 caatcaagat tttacacctt ctggttccca atttacaaat ggcttttcta tatttaatta
     2161 caatttttac gcagcaggtt tgaatttaaa aacagcacga ttattaagtg agtctataga
     2221 ttatatagaa atagaagatt tcattagacc tgcttttttg agaaaaaata gtcgtgtaaa
     2281 attaagaatt agctttgaaa aaaatacaaa gagaatagta ggtgtacaac tttgtagtca
     2341 agaagattta tctggactat tatcaatgtt ttctcttgct atagaagaac aaattacatt
     2401 agacaaattg aaaatgttag attatttatt tcatcctagt tttagccaac cctataattt
     2461 tattttaaag gcagtaacag gattaaacat tgaaacataa tcgttactaa atgaaatcaa
     2521 gaaatatact gtattactca attcattgaa aatatttatc ataattttta atagaaaaca
     2581 ctattaaaga gaaaaaagag aggtaaatca aatgaaaaaa tctttaaaac ttgcatcagc
     2641 agttatatta atgtcccaac tccttatagc aacacctttc catgtgttgg caagcgaaaa
     2701 ttcaacatac aaaattgcaa aagaaagaac cgagacgact gaaatgagta attccgatgt
     2761 aacagcagag agtagtgaac tttt
//
LOCUS       DACEPD010000169         2778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.179, whole genome shotgun sequence.
ACCESSION   DACEPD010000169 DACEPD010000000
VERSION     DACEPD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.179"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..197
                     /locus_tag="IW797_002737"
     CDS             <1..197
                     /locus_tag="IW797_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225479.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IW797_002738"
     CDS             314..811
                     /locus_tag="IW797_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1225480.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IW797_002739"
     CDS             898..1290
                     /locus_tag="IW797_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1225481.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2778
                     /locus_tag="IW797_002740"
     CDS             1274..>2778
                     /locus_tag="IW797_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1225482.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          518 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagc
//
LOCUS       DACEPD010000170         2719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.180, whole genome shotgun sequence.
ACCESSION   DACEPD010000170 DACEPD010000000
VERSION     DACEPD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.180"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IW797_002741"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IW797_002741"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1225483.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1065 a          420 c          556 g          678 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatca
//
LOCUS       DACEPD010000171         2640 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.181, whole genome shotgun sequence.
ACCESSION   DACEPD010000171 DACEPD010000000
VERSION     DACEPD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.181"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="IW797_002742"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="IW797_002742"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1225484.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          948 a          461 c          584 g          647 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaacttgg agataacatc
//
LOCUS       DACEPD010000172         2623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.182, whole genome shotgun sequence.
ACCESSION   DACEPD010000172 DACEPD010000000
VERSION     DACEPD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.182"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            36..197
                     /locus_tag="IW797_002743"
     CDS             36..197
                     /locus_tag="IW797_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225485.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="IW797_002744"
     CDS             210..713
                     /locus_tag="IW797_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ1225486.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="IW797_002745"
     CDS             773..1162
                     /locus_tag="IW797_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1225487.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..>2623
                     /locus_tag="IW797_002746"
     CDS             1149..>2623
                     /locus_tag="IW797_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAQ1225488.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT          779 a          434 c          603 g          807 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 gttcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tgg
//
LOCUS       DACEPD010000173         2551 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.183, whole genome shotgun sequence.
ACCESSION   DACEPD010000173 DACEPD010000000
VERSION     DACEPD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.183"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(994..1512)
                     /locus_tag="IW797_002747"
     CDS             complement(994..1512)
                     /locus_tag="IW797_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ1225489.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          830 a          463 c          362 g          896 t
ORIGIN      
        1 tttatcatat ttttactata ccctatactt ttacttggta tatttatgaa taatcgcaaa
       61 tttaccccta tactactagt ttgtaagtta gattaataaa aatactaaag ttatttgttc
      121 ttttattaaa tacacctaca acttataaat tgatggtatt tagataacac gaaagaataa
      181 aaataacagc tgatagtaaa gtttgttgac gattgctact caatcgaaag tgagttagtg
      241 cgtatgaact tgcttctgaa aacttatgaa atgaaagacc ttttacatct tgcagaaatc
      301 ggtactggga ttaaactaag caataaaaac taatgttccc ctcattcttt gaacttctac
      361 tatttgattt atttattctg ataaatgacg atttcacctt caggatcttc aaatcccgta
      421 tagaatttat ttaagatctc ttcagtatca gtaataggat aggcgatagg ccacataccc
      481 tttgatggtt agaataaact gctcaaaatc tgtatcgtat gttggcttta gcatattaac
      541 aaaggctatc tccaaattat aaaccataag cgttgaatcg ttcttaacaa tttcaattct
      601 tcctaaccca aagtcagaag atataattac cggacttgcc ttacccttca tgtgggttgt
      661 tgagattcca tatttaaagg gaattgtcgg tactatcggc ggtctgaaaa acatcccaaa
      721 aataaaaaaa tctttcccct gctagtctcc taagtccctt tgtagcggat atggcaccat
      781 aatctcattt tatcgaagtg agtcatggtt tatctgttgg ccaaccaaaa ttgatacaat
      841 ctaacgatgc accctctacg gcattctagg ggagtgcact tgcattcacc caataaaaac
      901 cggcagttag caaagaactt atgtagtcgt atgttcttac tgttaacata caagaggtct
      961 cctttccaca attgcttagc agtaaacggg aatttacaaa gctaatctct tccattttct
     1021 tatctttgtt ctaaaagtcc caaatggagc aactgtattt acatgaataa atttatatac
     1081 ttcccataca gccgttttgg ttgcctcgtc tgcccatttt cttttgtgtg cagtgaatag
     1141 atcttcatca ctcattgaat caatcatcat gttaatatca tcaatattac gattgagaat
     1201 ttcctctaat tctttcaatg gtagatgtgc ataggtattt gtaaaccact tatataactc
     1261 tcctaactga ttccatttaa attgatcaga aggcgtttta acagataatc catttctttc
     1321 atcattttcc cattttagaa ttaaattcgt ccaaccaact tgatatgcta aattttcagc
     1381 aggtgttcta tccacttcct caactctttt atccttcaat tcttcaggaa tagtttcaaa
     1441 ttctaaaata tatttagtat aagtcctatt tatttctttt tttaattctt ctttattttt
     1501 atatgttctc attatattac ctccataaat tcctaattgt ctcctttaat tcatctataa
     1561 tttcatccac gatattttaa tccgttctac aatgcatatc tgatatgcat tgactgtcta
     1621 actcatatgt gaatgattta taaaatatcc caacgtctca tctaaagaaa tgttgatcgt
     1681 cagcaaggag ctaaaaaata cgaatatatt tcttttgtag taaaatcgga ctggtttgca
     1741 tatcattttc ttcgtaaata tcaaaatcat gttaactact atcaattact aataaaaact
     1801 accactttaa gcgttaagaa ctgttaagtt tagttaagtg aggatagttc acaaaatcca
     1861 ttgttaaact tcacgaaagt ggtaaaactc aaaatgcttt atataggatt acagcatcgc
     1921 tctttcttcg attgcgcgtt gggtaaaaaa tattatgaag taaaaattga cgatgacacg
     1981 ttccttacgg ctcatcagat gaaacagcta caaaaacgaa atgatcaact cgaagaaaat
     2041 ttaattctaa aaaagcgatt gccatattca cgcctcgctc gacgaactat taaaggtcaa
     2101 gatttacctg aagatcatcc tttttaggaa attgaagctt tttatgttgc acaatgcatt
     2161 tataatacta caatgatgtt ttcaccagat attcttctag ggtgcggtgt aatgaaacag
     2221 atacacttgc aaaagaaaat tcaccgcgaa tttactaaaa ttatgaataa ttatgttaat
     2281 cttccgaaat ttttttccaa aattaaggga tcaagctggt gttatagatt gtttaaggtt
     2341 ggcaaaaaaa taacttcttt ttaaacaatt atagaaaacc gatactctgt taagttggtt
     2401 gacacggtgt cgatttttat gactttctta ttctgaaact tcactaacgg acagtaccta
     2461 aaaagtctat agtatggacg tttacgagta ctattttatc aaaaatgctg ctcatttcaa
     2521 cttatatgtt ttagtgaact acccccactt a
//
LOCUS       DACEPD010000174         2539 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.184, whole genome shotgun sequence.
ACCESSION   DACEPD010000174 DACEPD010000000
VERSION     DACEPD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.184"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(54..356)
                     /locus_tag="IW797_002748"
                     /pseudo
     CDS             complement(54..356)
                     /locus_tag="IW797_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1070..1360)
                     /locus_tag="IW797_002749"
     CDS             complement(1070..1360)
                     /locus_tag="IW797_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225490.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1568..2259)
                     /locus_tag="IW797_002750"
                     /pseudo
     CDS             complement(1568..2259)
                     /locus_tag="IW797_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          749 a          468 c          352 g          970 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcaacatac
       61 ttgcaaagta atactacaat tcctaacata atcgtagaat ttaataattt ccatagcata
      121 aagtgtttag aaaagaacat taagaatagt tctataataa ttcttgaact ccatgtctta
      181 taatgttcta tcgaagcacc caaatagcca agttcatcta atctacttgc ataaactacg
      241 tcatccgctc tcatagtcgg catatacata ttaaagagta aaaagatacc ccaaatccct
      301 attagtaaca agatcagttc tttgttattc gttatttttc tattatgttt gttcattttt
      361 atactccttc ttatatttcc taatagagta taacatagct ttaagcatta cttaaagata
      421 acttaaaagt tatcttttaa atacttaatt gttaaatacc ttatttgatg caaaagaaat
      481 ctacgatagt caacaaaaaa attttcatta tatccagaaa aatttttctt agttaaggac
      541 ctatggattg cagtaatgca gaattcgtcg aataaactgc caaagacata caatcatatt
      601 gcttttaaaa ttgacgaaca agatatagat tcgtttgtct caaaaatcca aatgttaggt
      661 ttaactgtcg aaccaggacg ttctcgtgtg aaaggcgagg cttcttcaat ttacttttac
      721 gactatgata atcacctctt cgagttacat accggtactc tccaggaacg tttaaaatcc
      781 tacaattcca ctacctgaga ctagtaatga ctagtctctt tttatggcct ctgatccccc
      841 cattgagcga atactaatag ggcttcgaat tgatctgttc aatctttaag caatatccct
      901 caaatgagtt tggaaagttt agtgattcat cacaaatact taaatacatg tagttatata
      961 tagattgagg ttcttgtgag cgtatgctcg tgtgggttca aatcccatcg tctgcatcca
     1021 tctttccaaa acaagcggct aggacatttt tgtctcagcc gcttgttttc tagtttattc
     1081 tttcatcttg tcggaccttt tttcttgggt atagactttt ctcatattct accacaattt
     1141 tgggtttccc taacacatga taggatctca caacaaattg ctttctatta ttataataac
     1201 ctgtaacgac taacttcatc ccttcgtcta cttcagctag gaagttcaaa ctatgggaag
     1261 caatcaaaca attgacccca tttaaactga aacggatcaa cggtctggca ctaacttgca
     1321 aagtacggat cttatctact gttccatatt ttttctccat aaccttcacc tcaataccat
     1381 tatacgaaca tacattccct ttgtaaagaa gaaactctgt tttatctttt tgactttttg
     1441 tctattacct ttcttaaaat catattggca cttcctgcga agatgaagga catgtactaa
     1501 aaaaaagagg cagcagtata gattactgtt gcttctttac taatttaggt ttacttgtta
     1561 agaaagatta tttacgcaaa tgttcttcaa caatttgctg agatttatca gctgtatctt
     1621 ccacttcatc ttttgctttt tctaactttt gaatatattg tttcaagctt gagttttcag
     1681 cagttgcttc gtctaatttc ttttgtgtat ctgttaactg atttctacat ttgttttttc
     1741 ttgtgtcagt gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag
     1801 ttttgcttgt gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc
     1861 atttttttgg ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg
     1921 tgcatccttg ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt
     1981 ttggttttct aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg
     2041 caatgtctgt tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc
     2101 tgtcaatgta tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc
     2161 accccacaaa ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa
     2221 tgcaacgaca atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa
     2281 tacttttaca tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca
     2341 tatgtaattt taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt
     2401 aaaaaatctt ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg
     2461 tgaacaaaaa caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact
     2521 gtatttatta caaaaaacg
//
LOCUS       DACEPD010000175         2517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.185, whole genome shotgun sequence.
ACCESSION   DACEPD010000175 DACEPD010000000
VERSION     DACEPD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.185"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..131)
                     /locus_tag="IW797_002751"
     CDS             complement(<1..131)
                     /locus_tag="IW797_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ1225491.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVC"
     gene            complement(205..486)
                     /locus_tag="IW797_002752"
                     /pseudo
     CDS             complement(205..486)
                     /locus_tag="IW797_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            948..2330
                     /locus_tag="IW797_002753"
                     /pseudo
     CDS             948..2330
                     /locus_tag="IW797_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          912 a          436 c          414 g          755 t
ORIGIN      
        1 tgacaaacat ttattccacg atcatataac aattcctgaa cttcacgata gcttagattg
       61 taacgcaggt agtaaccaac agcgacaata atgacgtctt ttttgaattg tttgccttta
      121 aaatgattca ttactctgtc ctctctgtct tttttctcaa ttttacacta aaatagattt
      181 tttggaaaac tttgcaacag aaccgaaatg agtatagtat gaattagtaa tttggttttc
      241 atctaagttt agctttgatt tactaaaatt gatatctcga tacatgttca atacatctaa
      301 tacaaatcga gaatcttctt tcggaaatgg ctcagaaaga tcactccaaa tttcatcatc
      361 ataatgatac tcgtaacctt ctctcaatat ttcgattttt ctttcataat aagatctgtt
      421 ttggtcctca aatttagcca ataactcaaa ttgatttact agtgacaatc tctgaaaact
      481 gtccattaaa tgtttcttcc tatcttgttc attaacaaaa aattataagc ccttacaacg
      541 aatattatat attaaattta attgtaggtg aacacgaatt ttataaatat ttatggtgtt
      601 aatgacaaat gatatccgac tcctcttcat acgttctgga ctatttgcta atagattaaa
      661 atttcagcat ctatttctga ccataaataa atgagctgcc gaaaagctgc caaatcagac
      721 ttgaaaataa aggatcaaaa acttttaagt ttctgacccc gtaactcaca cttcaaaatc
      781 ttacttaaaa aggagtctga aagaaaaaaa gagtattcaa aatttttttc taactagcta
      841 catcacatct atgcattgac actcaaagaa ccagaataag tcatcagaaa gcaagggggt
      901 gcggacataa tcctggacga aaaaataaac ttaggaggaa tgtccgtgtg ttttcccaca
      961 aagagcgtgt aagggcaatt cagttatttt taaaatacga ttgctcttat gctgcaacca
     1021 ttcgagaatt agggtatccg tcgattggtg cactacggaa atggtataaa gagtatttgg
     1081 aatccggaga gctccatcag gagcaccgaa aaaaatcaaa gtattctgaa gaacaaaaac
     1141 gaatagcggt gaatcattat tttgaatatg gtcaatgtta tgcacgaacc attcgcatgc
     1201 ttggttatcc taatagagag tccttgcggc agcggtgtga agaacttgca ccaggagcta
     1261 gaaaactccg aaaaagtgcg gtaaagttaa cacaagatca aaaagaagct gtgttgaaaa
     1321 aattctataa accgcaaact aataggaaaa atttagccga atctggagga attagtagag
     1381 ttactctata tcaatggaaa aacacggttc ttggaaagga ttttccattg tgtatgactt
     1441 taaaaaatca ggaaaaacaa aaagagctac ttttaaagga agtagaagac ttacaaaaac
     1501 aagtacacca attacagctc gaaaaagcat tactcgaagg agcggctgaa ctgctaaaaa
     1561 aagaaaaagg cgtcaatctt ctttgtctat caaatcaaga aaaaacgata ttgattgatg
     1621 ccttacgaaa tcagttcacc ttgaaagagc ttcttcaaca gctccagttt cctaaaagca
     1681 gctattttta tcaaaaacaa gcacttgaga aaccagataa gtattacaaa gaacgtcaaa
     1741 ttaattatca cgattttcaa tcataacttc tgtgcgtatg gttaccgtag aattcaccaa
     1801 gctttaaaaa atatgggtaa aaagctttct gaaaaagttg tacgtagatt gatgactgag
     1861 gaaaatctat ttgttaaatt ttctagacga aaaaagtatt cttcgtatgc tggggaaatt
     1921 tctcctgcac accctaattt attaaatcgg aattttaaag caaagaagcc aaatgaaaaa
     1981 tggttaacag atattactga atttagaatt ctcgcaggga aaatctactt atcaccactt
     2041 gtcgattgtt acgatggtgc tattgttagt tggactattg gaacaaagcc aacttgcttc
     2101 actctatgtc taaaaagggt tattcaccag ataattcagc gtgcgaaggt ttctttggac
     2161 gattaaaaaa cgagatgttt tatcaacgtg actggaagaa tacaacaatc aatcagttta
     2221 taaacaaatt ggatgattat attcattggt ataataatca acgaattaag ttatctttag
     2281 gagggctaag ccctcttcaa tatcggaaga agcaaagata tatttgttaa aaaggtccaa
     2341 aaaaaatatc cgcaccccct tttttttgta aaaaactatt gcacaataca agtagcctac
     2401 ggctatcgaa atttccataa ctttcgagca agaacttatc tcattcaagg cttgatcttc
     2461 caagtaaaac aaaaaccagt taagcactct gcctaactgg ttatgactaa attttca
//
LOCUS       DACEPD010000176         2399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.186, whole genome shotgun sequence.
ACCESSION   DACEPD010000176 DACEPD010000000
VERSION     DACEPD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.186"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            206..2068
                     /locus_tag="IW797_002754"
     CDS             206..2068
                     /locus_tag="IW797_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1225492.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2399
                     /locus_tag="IW797_002755"
     CDS             2068..>2399
                     /locus_tag="IW797_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1225493.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          715 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataatta
//
LOCUS       DACEPD010000177         2357 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.187, whole genome shotgun sequence.
ACCESSION   DACEPD010000177 DACEPD010000000
VERSION     DACEPD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.187"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            282..1322
                     /locus_tag="IW797_002756"
     CDS             282..1322
                     /locus_tag="IW797_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ1225494.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2146..>2357
                     /locus_tag="IW797_002757"
     CDS             2146..>2357
                     /locus_tag="IW797_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ1225495.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIP"
BASE COUNT          897 a          322 c          385 g          753 t
ORIGIN      
        1 tattttttgt tttgacactg gtagtactta attatcttta attttaaaac taaattttat
       61 tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt agttcttaat
      121 gactttaaag gtagtacata attcatttta tttctgattt taggtagtat ttaattaaaa
      181 aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa agagtaggta
      241 attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat tttaaatttt
      301 ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc tttattgttt
      361 ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt ttaaaagcaa
      421 gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt tattttactt
      481 atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg atagcaatca
      541 aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt aacaaacagt
      601 tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca gaaaatgata
      661 tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa tcagagggta
      721 tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg gctcaagagg
      781 gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa atcataccag
      841 aacttaataa taatatatta gacactaata gacacaatat agacactgaa aaagaccgcc
      901 tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac agcattgcta
      961 cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt tacagtgaag
     1021 ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata gaaagtggta
     1081 tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa gaattataca
     1141 cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat atccagaacc
     1201 tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct aaagtatcaa
     1261 gtgaaaaacg tttgaattat gaaagctccc caagcactat tacgaaagac tggttagagt
     1321 gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt aaaaattttt
     1381 tattattcta attatattac gaatattaca aaataatagc tattttaata gaatttataa
     1441 gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt gttaaaagaa
     1501 ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt tttttgagtt
     1561 ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat cttgatatta
     1621 cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat ttaattgtct
     1681 gctatgcttg tgatgaattg attttcgttc tgcacttttg gatgataaat ttggacaatt
     1741 gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct tcgtcagcgt
     1801 tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt acggtattac
     1861 ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt ggctgtgtta
     1921 aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcataatgtt ataaaaatgt
     1981 ccgtggggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat gtttgaatgc
     2041 gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga aaaataacaa
     2101 gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc atatacagga
     2161 attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac agagaaaatc
     2221 acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa atatataatc
     2281 cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa gcaaataatt
     2341 ttaaaagatc ctatccc
//
LOCUS       DACEPD010000178         2353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.188, whole genome shotgun sequence.
ACCESSION   DACEPD010000178 DACEPD010000000
VERSION     DACEPD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.188"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            91..363
                     /locus_tag="IW797_002758"
     CDS             91..363
                     /locus_tag="IW797_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225496.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            808..987
                     /locus_tag="IW797_002759"
     CDS             808..987
                     /locus_tag="IW797_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225497.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            1046..1402
                     /locus_tag="IW797_002760"
     CDS             1046..1402
                     /locus_tag="IW797_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225498.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT          845 a          327 c          447 g          734 t
ORIGIN      
        1 ttcctataca acgaaacacg tgttccaaaa aaacattata aagaaaaatt gtcggtagct
       61 gtggaagaaa tgattgaata aggtgtcgaa atgaatctta ttgcgacgta ctatcgaaca
      121 ctggaagaac tgaaaaagca aaatgcaaaa tggtttttcc aagccttact atgcttggaa
      181 gtaggtgtca aaccaagcac tattaaacct tctgaatacc aagctttaga actaacgtat
      241 gcgaaattta tcgaaacgaa aaaagccaag actgtttcaa gtgaatggtt agattatttt
      301 gaaaatatca ataagtatgg cgcatattac accatgaaaa aggaagacaa cgaaaatgaa
      361 taaggtgatt tatcaactaa caacggattc catatcaacg atttgaaaga aaaaaaggtc
      421 aataatcatt tataccgaaa agtgagaatg tgtgaaacaa ggacaaaaaa cgcctagtaa
      481 ctaaggaaat ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt
      541 gcgaaataaa cgggcaaagc ccgataactt aagagaaaga aggtgatcca tttgaacaag
      601 attattccct tattgttagt actgtttgtt ggcgaaatac ttatgcgaga tagtgcagct
      661 gtacagttaa acctcgctgt atttttggta atactggtgt tttgtttgat cttttggcgg
      721 tttgtattag tcctcgtgag tctactattc aagatgattg cagccatatt tttcataggg
      781 ttatttcgat gaaaggagtg agtaccaatg ccaattgcaa taggaaataa acggttaccc
      841 gtgacattag atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtatggcaaa
      901 agtgaatcta agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga
      961 tttgatgtac cagcgctaaa aaagtgacgt cactttttaa tttgatctat ataagtgata
     1021 tcacttttac caaggaggga attaaatgtt aaaaactaaa aagaattcgt acaaattaaa
     1081 gcttagtata ctatctatta tttgtctttt aggcatgata attagtggtc atttgtatag
     1141 agtggcatat acgcagttta ttgagtacaa ggttcactct ttaggattag ctggggatcg
     1201 atacgttgaa tatgtccatt attcattaaa atgttcttca tatattcttc tttggcttat
     1261 tttttcattg tttgaattgc taaatttaat agcaatatct tcatatcact tagataagct
     1321 aaataccaaa tctagccgtt ataacgaagt gtttaaggaa aatgtgaagt tagaaagtga
     1381 cttaaacaag ctaaaaaaat aaaaagaaga aaacataagt taactaaaat tattttatta
     1441 agaatgatgg aatataagga taaggttacc aacataacga tcattacttc tctgatatgc
     1501 tatggacttc tatcaatcag tattttgtct atagattatg gagtaactat actgcaagcc
     1561 ttagttttgg ctatgcctat gtatctatct ttgtttatca tgcatgaaat atataagcta
     1621 tttgttttat caaaaaatgt agaaatacgg gtaaatattt tggtgaatag ttatcaaaaa
     1681 gaactcaaag aaagaagacg gtggcggtat ttattgaaag gatgaaaaag taggatttga
     1741 catactggta ttcagaaagt gccgtcactt ttaaaacaat ggaaaaggtg gagataagat
     1801 gaagaaaaaa caaactaaac tatcattagc agatatttaa cacagttaac cgcaactgaa
     1861 gatggtgttg ttgaatttga acaatctgaa attgcggtag tagatgatcg ttaccttaaa
     1921 atgccttact tctttgacca agcaaaggtc atttgccttt gtcactacga tggggtacgt
     1981 gattttaggc gttaaaaagt agtgattata tcaactttac gagtaaatat aaatttagac
     2041 atgaaactga attatccaat ggaaaatcat ggtttataat ctagaaaaat tatagtaatg
     2101 attggattat atatattctt tcgtattttc gtacaactgt agcttgtcaa aattgagaaa
     2161 tttaaccgat taggtacttt acatcgttat ttgggtaatt tctaaaacat gaagcttacc
     2221 aacgaataaa ctgagactat cttattaatt gatcaatata agaaaatatc tttgaggaga
     2281 tgatgtacta tgttttcgtc tatccacgat tggttaggtg gtcttttcgg cggtggaggt
     2341 aaaggttttt aat
//
LOCUS       DACEPD010000179         2189 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.189, whole genome shotgun sequence.
ACCESSION   DACEPD010000179 DACEPD010000000
VERSION     DACEPD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.189"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(325..528)
                     /locus_tag="IW797_002761"
     CDS             complement(325..528)
                     /locus_tag="IW797_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225499.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(987..1952)
                     /locus_tag="IW797_002762"
     CDS             complement(987..1952)
                     /locus_tag="IW797_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ1225500.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DACEPD010000180         2183 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.190, whole genome shotgun sequence.
ACCESSION   DACEPD010000180 DACEPD010000000
VERSION     DACEPD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.190"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IW797_002763"
     CDS             complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IW797_002763"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ1225501.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          581 a          475 c          397 g          730 t
ORIGIN      
        1 tttatcgaga ggttgtgaaa tcagcgaatt aacctgagta ttagagaaaa aaatggaaaa
       61 atagtccctc atattttacc gtttgttgaa ctaataccga gggtcagctg attgaacacc
      121 gtttagtgga gaggtcgtaa gcctaccccc cagctcctag ttacaaggac aaattcaaaa
      181 aacagctctt catattttat gaatgttgta cttgtgattc ggaggtggca agggaacact
      241 gtttattcaa aaagaggctg ggacagaagc gtttaactcc aagaaataag aagaaattca
      301 cgaaaattgc ttttcaaatt tttgtgaatt tcttcttatt tccgaaggag ttgcttctac
      361 ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
      421 ctctttaatg tttttaagat tcaactgaat agtttggtgc ttcttttgtg atttgtacat
      481 catgagggtg tgattctttt aatccactgc cgctcatttg gataaattgc gcttcatcac
      541 gtaattgctg tagattggct gccccaacat aacccattcc tgaacgcaaa ccgccgatca
      601 tttggaaaat aatatctgca acacttcctt tataagcaac ccgaccttcg attccttctg
      661 gtactaattt atttgcttca ttgacactac cttggaagta acggtctttt gaaccttttt
      721 ccatcgcgcc taatgagccc atacctcggt atgttttgaa tcgacgtcct tggaagattt
      781 cgaattcacc tggtgattca tctgtccctg ccaacatact tcctagcatc actgcatgtc
      841 cgccggcagc taatgctttg acgatgtcac cagaatattt gatccctccg tctgcaataa
      901 tcgctttgcc atattgacga gcaactgatg ctgcatcata aatagctgtg agctgaggta
      961 cacctacacc ggctaccaca cgtgtcgtac aaatcgatcc tggtccgata ccaactttta
     1021 cgacatctac acctgcatca tataaagctt ttgtcgcttc agccgtcgca acgtttcctg
     1081 caatcaatgt tgcatctgca aaagttgaac ggatttcttg aatttttcgg atcacacctg
     1141 cactatgtcc atgagctgta tcgatgatga ttgcatctgc ccctgcttct aataaagcat
     1201 tcgcacgctc aaatgtatcg cttgttacac caacggctgc tgctacaagc aatcgaccat
     1261 gttcatcttt tgctgcattt ggaaattcga tcactttctc gatgtctttg atcgtgatca
     1321 atccgcttaa acgaccttcg ttatctacga ttggtaattt ttcgatttta tgtttttgca
     1381 aaatcttttc tgcatctttt agagaggtac ctactggggc agtcactaag ttgtcttttg
     1441 tcatcacatc agcaatcggc atgtgataat ctgttacaaa acgcatgtcg cggtttgtaa
     1501 ttatccctac caattttctg ttttcaagtg tttcaacgat tggcacacca ctgatacgat
     1561 atttgctcat caattcttct gcgtctgcta caagatgctg tggcgttaag aagaacggat
     1621 caatgattac gccactttca gaacgtttga cttttcttac ttcatccgct tgctggctga
     1681 tcgtcatgtt tttatggatc acacctagtc cgccttgtct tgccatagca atggccatct
     1741 tgctgtcggt aacagtatcc atacttgcac tgatgattgg aatattcaac ttaatatttt
     1801 tagctaattg tacgctcata tccacttcgt ttggtaacac gtgactttct gctggtatca
     1861 ataagacatc atcaaaagta aatccttttt tcgtgaactt tgtttcccag ttggacattt
     1921 ttagtaccac tcctctaatt tagtttatct attaaaataa ttgatttttt catttaagtc
     1981 aatgatctac ttggaatcaa gttagctttc tcatagaata ccaatataaa gcgtttacat
     2041 ctgtgtatat taaaagagat tgtaaaaaag ctgtcctgca tcaagtaata agaacagcca
     2101 atcaaaaaca gcttctcatg tttttgaatt ggttgggttt attaccgcag atgcaaactt
     2161 ttgaacaccg tttagtggag agg
//
LOCUS       DACEPD010000181         2180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.191, whole genome shotgun sequence.
ACCESSION   DACEPD010000181 DACEPD010000000
VERSION     DACEPD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.191"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(209..1549)
                     /gene="glnA"
                     /locus_tag="IW797_002764"
     CDS             complement(209..1549)
                     /gene="glnA"
                     /locus_tag="IW797_002764"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ1225502.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1577..1969)
                     /locus_tag="IW797_002765"
     CDS             complement(1577..1969)
                     /locus_tag="IW797_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ1225503.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          619 a          483 c          368 g          710 t
ORIGIN      
        1 acaagaaaag ctgtttttgc ttggctattc ttattacttg ttgcagggca gctttttcac
       61 aacctctcca ctaaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
      121 aaatatgagg aactattttt ccgttagttt ctctaatact caggtcaaaa cgctgatttc
      181 acagcctcta attcacggta ttcctgtttt aataaagttc tagatattgt tctctttccc
      241 actcggaaac agtttgtctg aatgccgccc attcgagacg ttttgcttca acaaaatttg
      301 agaagatatg ttgtcctaat gcgtcaatca ttacattatc cttacgcaat tctttgatag
      361 cattatggat agttgaaggt aagtcctgga tatgtgcagc ttctctttct tcctcatcca
      421 ttacataaat gttacggtca acagctgctg gcggtgtgat ctgattacgg ataccgtcta
      481 gtcctgcttg caacaatact gccattgcca agtatgggtt tgcagatgga tcaactgacc
      541 gcaattccaa acgtgtggat aaaccgcgag attctggtac acgtactaat ggcgaacgat
      601 tacggccact ccatgcgaca taaacaggcg cttcataacc tggaacgaga cgtttataag
      661 aattgactgt tggattgcaa acggctgtgt aggcacgagc atgtttcagc aaaccaccta
      721 agaaatgata agctgtttca cttagttcca attcaccgtc tttatcatag aaaacatttc
      781 cttcatcatt gaacaaagac atattgcaat gcataccaga accattgatc ccaaataacg
      841 gtttcggcat gaaagtcgcg tgaagtccat gcttgcgtgc aatcgttttg acaacgagct
      901 tgaaggtttg gatattatca caagcatcta ccacatctgc atatttaaag tcaatttcat
      961 gctgtcctgg cgctacttca tgatgtgaag cttccacttc aaagcctaga ctttctaatt
     1021 ccaacacgat gtcacggcgg caattttcac ctaaatctgt tggtgcaaaa tcaaaataac
     1081 cacctttatc attcaaggtc gttgtgatcc caccgtcttc gtctaattta aataagaaga
     1141 attctggttc aggtcctaaa ttgaaagagg taaaaccaag gtcgcgcata tctttcaatg
     1201 ctcgtttcaa attcccgcga ggatcgccgg caaatggtgt cccatccgga ttatagatat
     1261 cacaaattaa acgagcaact tttccatgag cactttccca cgggaagatc atccaagtgg
     1321 agagatctgg atacaaatac atatcgcttt cttcaattcg aacaaatcct tcaatcgaag
     1381 aaccgtcaaa catcatttta ttttctaata ctttgtcgat ctggctcact ggaacttcta
     1441 catttttgat cgtacctaag atgtctgtga acatcaatcg taaaaatcgt acgttttctt
     1501 cctcaatgat tcgtttgatt tcttcacctg ttatctgttt ctttaccatg gtatccctct
     1561 tcctttttta atttattcag tcaaagaatt gtctttgcaa tccttgcgac tgataatgac
     1621 ctgttttgct taacccacct tgcgccaaaa tctcatcacg aaaaatttgt cgaacgtcat
     1681 gatctgtcaa cggcttcttg ttttttgctt cttgtttttt cgcctgtgcc atttcgtata
     1741 cacgtttgat cccagccata ttcaatccct ctgaaagata gtctttaatc tcaagcaaca
     1801 tatcaatatc attcagcgaa tacatacgtc ggtttccttc gcttcgttcc ggatgaacta
     1861 aatcttgttc ttcataatag cgaatttgcc gagcagacaa atcagtcagc ttcataactg
     1921 taccaattgg aaatacggac atagatcggc gcaattcctt ttctcccatc agacgtcccc
     1981 tttcgtttta taagttggct aaagtgtacc aattaaaaaa aatattgtca actgcatatg
     2041 ttaggttttc taacatgaga tctctatttt ttataaaata acaaaaaaat aagaacacaa
     2101 aaaaggtatc tagttgattt tcgtaccaga taccttttat tttttattat actgtactat
     2161 cctttattag tgtttttatt
//
LOCUS       DACEPD010000182         2155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.192, whole genome shotgun sequence.
ACCESSION   DACEPD010000182 DACEPD010000000
VERSION     DACEPD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.192"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            181..483
                     /locus_tag="IW797_002766"
     CDS             181..483
                     /locus_tag="IW797_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225504.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(999..1072)
                     /locus_tag="IW797_002767"
     tRNA            complement(999..1072)
                     /locus_tag="IW797_002767"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1036..1038),aa:Ile,seq:cat)
     gene            complement(1627..1941)
                     /locus_tag="IW797_002768"
     CDS             complement(1627..1941)
                     /locus_tag="IW797_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225505.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          794 a          325 c          333 g          703 t
ORIGIN      
        1 ttttataagg gtttgagcat tttttatttt cgcagatttt cgttaaatga atgaaaatgg
       61 cattcaaaat agcattcaaa aagaacactt tttaatatat atgttccatc catattatca
      121 atgatattcc tactttacaa aaagaacatt agttcgtata ctctttttga ggtgaacatt
      181 atgttgatgg aaggaaaaac acaattatgg tttaaatttg atccttcgaa tagatttgtc
      241 aaagattttt ataaggtgtg ggattcagaa gttttctttt tagcaatcga agatagctta
      301 ttaatcaatc tctattactc taataagaac tactttaaaa ttccggcaac aaaaaccaga
      361 atgaagaaag atgtgtattt tttgtttgat atcgtgactg atgtgccaga cgcacggagc
      421 gatcatcggc gttatgacta tttgaagtat actttcgttg atccagaaag gtataaagat
      481 taaagtaggc tacctaaaaa aggtagcccg gaacggattt tatcaccata cttataaaag
      541 ggatattttt taagttagta ttaagattgt gtaatatgat gatatctata ttttatagta
      601 tcagtgctat aaaatcaaaa ataagtcact aattaactac cactccaatt ataagtcttt
      661 ttcccattat tttttacagt tatatggtat gctttttaat ggcttcaaat ataaaagagt
      721 ttaaagcgta acacacttat ggggaagtgg tttttgggga acgctttaaa ctcttcttta
      781 ttattatctc acaatataac ccaaatgtct ttccatttaa aaatcaaagt aaaacttttc
      841 aaatatacag aagtataact atgtgaaaca tcctttcatt aatccataaa aggatacata
      901 aaaaagccac tcatttgagt ggcaatgaag aaaagcttta gcttgtataa tactcttcaa
      961 aaaattctaa cacagaacga ttcaattggc tacattaatg taccctgtag gactcgaacc
     1021 tacgaccgga cggttatgag ccgtctgctc tgaccaactg agctaagggt agtggttgtt
     1081 gccacataaa gccataaaca atcaaccagt agaatgcgtg gcaacaaacc tgttatcgca
     1141 tatcttggag tgtgactatt tatgagtgat agtgaagata tgcgtctcca tatatgtatg
     1201 gttgagacag ataaacatgt atgaaaaacg ttgttaaatc aacgtttttt tattttggtc
     1261 aaggagagaa aagtagagaa atacaaaatt ttttgcattg attttgcatt caaaaaaaca
     1321 ttcaaacatt tagaaatccc caatacgtat tcaggaaaat ctattttaag gtttacaaaa
     1381 agaacttttg ttcgtacact ctttttgagg tgaactttta tgctgatgga aggaaaaaca
     1441 caattatggt ttaagcatgt tccttcaaat aagtttttta attataaact ctagagaaat
     1501 taattcatcc taacataaaa taattgttca ctcaaaagtt tttctagaac aattaaaaca
     1561 gcaggcttca cattcgctat acatttgtaa gaagtaataa ttcctgtact aaaaggcata
     1621 attgctttaa ttattttttg acttctcttc aaacacaaga agtagttgtg taataattgc
     1681 agaaataagg attccaataa tagatataaa atttgacatg gaaacgtcta taacattaaa
     1741 agaataaagt gttgaagaaa gtaaactgat tgaaataacc cctccgtaaa aaatagtctg
     1801 tgaatatacc cacttttttt cagaagacgt acttcgtttg gttctaaaac ctatccctgc
     1861 atttctactc ttatcaaaaa accaaatatt tagaagtaat aatattgcta ttattaagat
     1921 aaaattactg taaaaatcca aaagctatcc gagagtataa aatattttgt gtaaatgaaa
     1981 aaatccatac aaaaaaggaa gtcccttctg tagaataaag ttaacgacaa ccaattcaca
     2041 gaaaagagga cttccctatg aatgatttta ctacagaaat tgtgcaaact ctagtcacta
     2101 aaggcgattt aaatgaatta ttccgttcgc acttagaaaa agcgataaac acact
//
LOCUS       DACEPD010000183         1889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.193, whole genome shotgun sequence.
ACCESSION   DACEPD010000183 DACEPD010000000
VERSION     DACEPD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.193"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            273..1751
                     /locus_tag="IW797_002769"
     CDS             273..1751
                     /locus_tag="IW797_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ1225506.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          694 a          261 c          331 g          603 t
ORIGIN      
        1 aaagagcagg acggtggccg tcctgctctt ttttgtggtt gtttacgcaa atggcgcaaa
       61 tcttgtacat tttaacacaa taaaacaaaa aatatagaat atttcctcat aaatttttcc
      121 attttttgca atattctcaa aaaaaagata aaaaatgaga ataactgatg aaaaaacaag
      181 cataatccat cataaaatca gtataggtga aaagatgaac tttcatctgc atatcaagta
      241 attggtatga ggcatttttt gaggtgtagg atatgaagtt ggattacctt atagaagagg
      301 aagatagaga aaaactgctg ctagctagat atttagaatt tgagaaaaag aattatgtag
      361 tgctgcctaa gttatatgaa acattaggtt tgtctaaatt taaaacaaga aattatatca
      421 tagaattaaa taaagaattt actgaattca aaacaaagcc agagatccaa atttcagaag
      481 aagcagagat ccatgtcgaa cgtctgaatt tgacggatat caaaaattta agaagaatct
      541 actttcaacg atcaaaaatt gctcgactgt taacggagat cgtggattca aaagtaacga
      601 tcgaaaaatt tgcagagaat cattttttaa gcatatcaag agcctatgta aaaagaagag
      661 aattagtctc ttttcttgaa cagcatcaaa taaagttaaa gaaaaacata ttagttgggg
      721 aagaaataaa catacggaac gtattatttg gggtatattt cggggtcttt aatggttttt
      781 ctatgccatt tagagaaagc gtcatgaatg aagcaaagag tatcgtgcaa tattttgaat
      841 atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa attcaatttg ctaaccacta
      901 ttagtttgtt gagggcctat gggaatcaac caatcggaaa acgtttcttt tcagatgaat
      961 actttaagag ctcagaagga atgaagatca tcaaggattt atcacatatt tcttttcttc
     1021 atatccctaa agagcagatc attgatgaga tttcttatat catgttgttt ttgatcacgg
     1081 aagaagaatt tgaattagat ggcgcatttg gacttgcgga agctcatttt catgatatcc
     1141 acatgattag tcaaaaaatg tcagtggaat tgattgatca attaccattt tcgattgaaa
     1201 tctctgaaga agaaagacta tatatacgaa aagaacttcg tatgggtatg atgcatgtca
     1261 atcgaaaaaa ttggatattc gactttgaga tgactagctt tacgtctaaa aagcagctac
     1321 agtttttctt cgagacgtat ccaatattca gtacagcaat ccaaaaggtc atacaagaga
     1381 ataaacgcta tttcttggga aaagatgaac gtttcatgat tcgcttattt tatgattatc
     1441 tctttttatt ggtcggtatt tgtccggtat catattttga agcaccgatt catgtctgta
     1501 ttgatttttc gcaaggatct gcttataccg attacattat ttctcaagtg aaaggattta
     1561 aaaattacaa tattgtgatt gagagtcgaa tcacttctcg tacgaatatt tatttatctg
     1621 attgtttact tgaaaaattt aataaagaac agataatttg gaaaaatcca ccgactccag
     1681 atgactggga atattttgga aataccatca tcagagaaaa aaatagacat ttaactgaaa
     1741 aaatcagcta gtatttatgg atggcagctt tgtctcacat atttattagc tgatttatgt
     1801 ttttttcaaa gaaaaggtaa ctagatataa ctagatataa ctagatatat tacttaacaa
     1861 aattaaaaag tacataagtt attattccg
//
LOCUS       DACEPD010000184         1859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.194, whole genome shotgun sequence.
ACCESSION   DACEPD010000184 DACEPD010000000
VERSION     DACEPD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.194"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="IW797_002770"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="IW797_002770"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ1225507.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DACEPD010000185         1845 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.195, whole genome shotgun sequence.
ACCESSION   DACEPD010000185 DACEPD010000000
VERSION     DACEPD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.195"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            311..1603
                     /locus_tag="IW797_002771"
     CDS             311..1603
                     /locus_tag="IW797_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1225508.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
BASE COUNT          593 a          308 c          365 g          579 t
ORIGIN      
        1 ggtctatttt acttttttat attttgcact gggatgaaat aaattaacca aaagtttctt
       61 tttcttataa tgtgtatcaa tattaaaact gaaaaagata tggataatag gatcaaagcc
      121 ctcattggaa cactcaatat attatcagat ttaaatttca aatagataat ttcattgttt
      181 agtaatatcg aaagatactt ttttaatgat gagagtgtaa tataaaccgt gtaagtaacc
      241 attaatccta ggcaggtggt tgcttcgcgg tttttctttt tctacaataa gatcaagagg
      301 tgattacacg atggctagaa agaaaagaaa tcctgatgcc gaaaagttag ctgaatccat
      361 tctgaatgcc tatcaacctg aatctgtcga tgacatgcaa gatgctttga aagatgtgtt
      421 tgggcccctt tttgaaaaaa tgcttcaagg agaattgaat aatcatttag gttatgatgc
      481 ccattctaaa gagcctaagg aacacgataa ccgtcgaaat ggctatggaa ctaaaacgct
      541 taaaaccagt tttggtgaag tagctattga tgttcctaga gaccgggaag cttcctttga
      601 accagagtta attcctaaga gaaaacgaga tgtctccgac atcgaaggga aggttctttc
      661 catgtatgca cgaggaatga gtcaacgaga tattgccgca accgtcgaag ctatttatgg
      721 ctttgatatt tcccatgaaa tgatttcaga tatcactgac gctgtccttc ctgaattgga
      781 agaatggcaa gcccgccccc tagcaaagtg ttatgccttt ctattcgttg attgtatgta
      841 tgttacttta cgagaaaatt atgaagccaa agaatatgct gtatacacca ttcttggcta
      901 tgatctcaaa ggaaataagg agattttagg attatggtta aatcaaacag aatctaaaaa
      961 tcgctggatg caagtatttg atgaactgaa agcacgtggt gtcgaagatg tctttttcat
     1021 ttcgatggac ggtgtttctg gcctcgaaga aggcgcaaaa gcgatctttc catcggtaat
     1081 tgttcagcgc tgtattgttc atcttgttcg aaatgcattg cgctatattc caagtaagga
     1141 ctataaggaa gtctgccgag atatgaaaaa gttctacggt gcttcttctc taaacgctgc
     1201 acatgctgct tttgacagtt ttcaaaatcg gtggtctcat tattctggtg ctgtagatgt
     1261 ctggaaacgg aactttgcac atgttgaaca actatttgat tatggtagtg ccattcgaaa
     1321 aattatgtac actaccaatg cggttgaaag tgtccactcc agcttcagaa aagtcacaaa
     1381 aaaaggagcg ttctctaatg agaatgcact cctaaaacta ctttatttac gcacgaaaga
     1441 attacacgcg aagtggtcag gtggtcgaat tcaaaactgg gctatggtgc ttaatcagtt
     1501 aatgatcaac gagaccttct cctctagaat gaaaagtatg cgatttacct tccgtaacaa
     1561 cggtaggttt attcaactat gtgtccgatc aaaatttatc tgatttcaaa atagccgaga
     1621 gaattatggt ggttaaaaac tcttaactat ttaattacgg ttttcatagt ttttggattc
     1681 aggaggatta aaacgaattc tataaaaaaa gatttggttt aaatgaatga ttaaacgaca
     1741 atttagcaac gtagtcagag taaaacagct ctttcttttc gtatttttta aagttgttac
     1801 gaaaaagttg ctataaatcc atctaagact gaataaatct tattc
//
LOCUS       DACEPD010000186         1747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.196, whole genome shotgun sequence.
ACCESSION   DACEPD010000186 DACEPD010000000
VERSION     DACEPD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.196"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            133..507
                     /locus_tag="IW797_002772"
     CDS             133..507
                     /locus_tag="IW797_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ1225509.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="IW797_002773"
     CDS             complement(621..1250)
                     /locus_tag="IW797_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ1225510.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="IW797_002774"
     tRNA            complement(1384..1456)
                     /locus_tag="IW797_002774"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
     gene            1670..1743
                     /locus_tag="IW797_002775"
     tRNA            1670..1743
                     /locus_tag="IW797_002775"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1704..1706,aa:Asn,seq:gtt)
BASE COUNT          603 a          256 c          307 g          581 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataatagg ataacgaata ttaatgtatt
     1501 tgtcaacaga attttttcga aaaaatgtgt ttctctaata ggaaaacaga ttttagaaaa
     1561 aataaaaata acaaaaaatg cgaacggttt ctttgataat ataataaaaa aattcaaaaa
     1621 aagtattgcg ttatttagaa gaaattgata ttatattcat gtacttgatt ccggcatagc
     1681 tcagttggta gtagcgcatg actgttaatc atgatgtcgt aggttcgagt cctactgccg
     1741 gagtaaa
//
LOCUS       DACEPD010000187         1702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.197, whole genome shotgun sequence.
ACCESSION   DACEPD010000187 DACEPD010000000
VERSION     DACEPD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.197"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(168..1460)
                     /locus_tag="IW797_002776"
     CDS             complement(168..1460)
                     /locus_tag="IW797_002776"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ1225511.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          487 a          360 c          293 g          562 t
ORIGIN      
        1 gtttttgtca cagtccctta ttctgaataa acagtgttca agagacagcc tatcggcaat
       61 aaggctaaac gtctctttca ctacctcttc aagttattat tatcgtatag ataaacactt
      121 cgactcgctc tggtaagaac gtcctgattc agtcgtcgtt tttatcctta gatttgcgcc
      181 cagacgcttt ctaagatgtt tgtttgtgtt ctatcaggtc cgacagaaaa tgttgagata
      241 cgtacaccaa caagctctga gatacgacgt acatagttac gtgcgttttc tggcagttct
      301 gcaagtgttt tgcatcctgt aatgtcttct gaccagcctg gcaattcttc atagattggt
      361 ttgcagcggt tcaactcttt caagcttgct ggatagtggt agattagttc gccatctaat
      421 tcataagccg tacaaatttt tactgtttcc aatccactta atacatcgat cgagtttaaa
      481 gataggttgg taatacctga aactcgttta gaatggcgca taactacaga atcaaaccag
      541 cctacacgac gcggacgacc agttgtcgta ccgtattctc gtcctacttc gcggatttgt
      601 tctcctgttt catcaaataa ttcagttgga aatggtccat caccgacacg tgacgtatag
      661 gctttgcaga cgccgacaac tttattgatt tttgaaggcc ctacaccgct tccgattgtt
      721 accccaccgg ctactgggtt agaagaagta acaaaaggat aagttccctg gtcgatatcc
      781 agcattacac cttgtgcgcc ttcaaacaac acgcgtttgc ctgcatccaa agcatcattc
      841 aagataacgg aagtatctgt gacgtattgt ttgatttgtt gtccgtattc ataatattct
      901 tcaaaaatat cgtcaaaatc aagtggttct gaatcaaaca tttttacaaa ttgacggttt
      961 ttgtcatcta gattcaaacg aagacgttct tcaaaaattt ctttatctaa taagtctgca
     1021 atacggatac ctacacgagc tgctttatcc atataggcag gtccgattcc tttaatcgtt
     1081 gtaccaatct tattttcgcc cttagcatct tcttgtaatt ggtccaattt gatatgataa
     1141 ggcaagatta cgtgcgcgcg gtcagaaata cgcaaattac ttgtttctac acctttttca
     1201 tgtaaataag ctaattcttt aaccaatgat ttaggattga ctacaacgcc gtttccgatc
     1261 acactgatct tgtctttata aaaaatccca gaaggaatca aatgcagttt ataagtcaca
     1321 ccgttaaact taattgtgtg ccctgcattg tcgccacctt ggtaccgtgc aatcacttca
     1381 gcattttcac tgagaaagtc tgtgattttt cctttccctt catcgcccca ttgcgtacct
     1441 actactacta cagatgacat gtgaacacct cgttctatat tatttaatcc tgaaccattg
     1501 tatcaataat agatacatat ttcaaataaa atcgaatgat ttttattttt attttataaa
     1561 aacgcttaaa aaacgaactt tatcttttta gaatggttat taaaaaagag gttgtaacag
     1621 aagtggtcag cttcaagaaa taagaaggaa tgtacgaaaa ttgcttctca aatttttgtg
     1681 aaatttcgac ttatttttcg aa
//
LOCUS       DACEPD010000188         1682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.198, whole genome shotgun sequence.
ACCESSION   DACEPD010000188 DACEPD010000000
VERSION     DACEPD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.198"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            517..1602
                     /gene="xerS"
                     /locus_tag="IW797_002777"
     CDS             517..1602
                     /gene="xerS"
                     /locus_tag="IW797_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ1225512.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          627 a          285 c          233 g          537 t
ORIGIN      
        1 tcgtactttg ttgatgattg agatattgct acatcaatct cagcagttaa gatttttttg
       61 aattgttgct tgaatttttt ctttatctaa aaaaattgta tctccaggat gcaacccatt
      121 ttctttaaga aattcctttt aaattgttaa tactactgag tgccccactt ctctgaattt
      181 tctttaactt ctccctcccc ttttttattt gtatatacat tcatatacgc ttgttaatat
      241 caaatcaacc gtagtctctc ttatgtctaa ttatatagaa agattgttct ttctatataa
      301 tttagacaat ttaaaaaatt aagattgcct ccttctgttt cctttcctat tcatgtatac
      361 catttatttt aaattattaa atctatacct atttatctgc ttttttgaaa taaattaata
      421 aatttcagag tataccaatt gacaaaacac tctataattg gtatagtcca attatagaga
      481 tactcctctc ccctacttat cgaaaggatg aataacatga aaaatacaga aatatatcaa
      541 aaaatagata caatcatact tcatatgccc gattatataa aagattttgt tcaagatcgt
      601 gaggataaag atcaatcacc tcgaactact ttagaatatc taaaaaatta taagctcttt
      661 tatgaatggc ttctgagtga gtctatcgtt cccgatactt caatcacctc gataagacag
      721 ttaactgcct atgatctaaa cttgtacaaa tctcatttga aacgtcgcac aaaggaaaat
      781 gtaaaaaaag agacaacaaa aactaagctc aaagataata ataacttagg tctatctact
      841 tctactatca atagaaatat cacagcatta aaggttctat ttaaatatct ttcgaaaagt
      901 tccaataatc ctttaggaaa accatactta gaaaataatc ctatggatca agtagcaact
      961 atcacagata aaactacgtt agctgcccgt gcaaacgcaa tcgagaaaaa attatttcta
     1021 gacgaagata cacaaaatta tctagattat attgccaatg aatataaaaa aactttgagc
     1081 aaacgagctt tgatctatta tcaacgagat gtagaaagag acctggcgat caatgcattg
     1141 attttaggat ctggattaag actctccgag gttgtaaata ttaatttaga ggacctatct
     1201 ttagaaaaga ataatgtggt agtaactaga aaaggaaata agagagacgc agtaaatata
     1261 gcagcatttg caatggaata tttagctaat tatctagcta ttcggaaaga acgatacaaa
     1321 gtaacagaaa acgaaaaagc tttatttcta gcaatttatc aaggagaagc caagcgtata
     1381 tccggtattg caatcgaacg gatggtagca aaatattcaa aaggattcag agtccaagtc
     1441 agtccacata aattacgtca tactttggct actagactct atcaacagac aaattcattg
     1501 gtattaactg cccaacaatt gggacataca agtacaaata caacaacttt atatactcat
     1561 atagacaatg cagctacaat tgatgctttg aacgctttgt aaatgtagta aaaaatagta
     1621 taataatgaa gcctgtctct aaaattagat aaaagcccaa ctttaaaggc aagcttcatt
     1681 at
//
LOCUS       DACEPD010000189         1661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.199, whole genome shotgun sequence.
ACCESSION   DACEPD010000189 DACEPD010000000
VERSION     DACEPD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.199"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(277..732)
                     /gene="tnpA"
                     /locus_tag="IW797_002778"
     CDS             complement(277..732)
                     /gene="tnpA"
                     /locus_tag="IW797_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ1225513.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            975..1661
                     /locus_tag="IW797_002779"
                     /pseudo
     CDS             975..1661
                     /locus_tag="IW797_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          515 a          324 c          307 g          515 t
ORIGIN      
        1 attatttctg cgccagcatt ttctaatgtt tgaatttgtg ttttgtaatc ttggttgctt
       61 gtgctaattc ttgaatagcc gtaaattgcc aaaatttcat caccctttct ggacataagt
      121 ttctgacacc agttaaaatc aagtctatca gtaacccata cacccgtgag gacgggtgta
      181 tttttaatcc ccctagaagg ggtcgacact cgctgcccct taaaagggga tttttaaaag
      241 accgccaact gctcatcttc actcacttac ttctctttat tttttaaatg gttctacgta
      301 ctctctttta cttataatat atcgaatgcg atcatcagct tcttgttcac ggatgtattt
      361 ttcatttgtc ttttgattca atccgactgt actcacatag tatctcttcg accaaaagga
      421 tttgtttccg tatttatatt tgagatttgc gtgctgctcg tggattagta ccgcactacg
      481 acctttcaga taccccataa aactggatat acatattttt ggtggtactc gaacaagcat
      541 gtgaatatga tctagcattg catgtgcttc tatgatttcc acatctttca tttcgcacaa
      601 cttcctaagt atttttccaa gatcttttcg taattgccca tagattactt ttcgttgata
      661 ttttggtgta aaaataatat gatacttaca attccaacga gtatatgcta aacttttatc
      721 gtcatttgac atgaaaaaac tcctttcgat ctgatgagcg gttggtagcc actcactagt
      781 atatcgaaag gagtttttat cgctaaagat acttcttcca cgcgtagaaa cgcgcggttt
      841 tcttgtttcg cacgctttac gcacaaaact gactgaagtc attaagaaaa taaaagggtc
      901 taaaactttt aagttttgta ccgaccccca aaagttagac caaaaaatct aacttttgga
      961 ggtcggtatt ttttatggct aagtatactt ttgaacttaa attaaagatc gttcatgatt
     1021 acttggatgg taaaggcgga agtgattatc tggcaaagaa atattccatt aaggctccat
     1081 cacaagtgaa gcgctggatc aatgcctatc aagaatttgg tgaagagggt ctagtaagaa
     1141 agcgacaaaa gaaaaaatat tctgttcaat ttaagctgga tgcgatagag ttatatctaa
     1201 caagtgagct gtcctatcgt gaagttgcca acacacttaa catgaacaac cctaatttga
     1261 tcgctagttg gatgcgtcaa ttccgtgagg gtgggatcga tggactctca aaaacgaaag
     1321 gatgtccacc tatcttgtct aagaaaaatg aacccaaaaa taagaaaaaa tctacaacta
     1381 aagcaacgtc taaagaacgt gaacgcatcg aggagttgga gaaacgagtc cgttcccttc
     1441 aaattgaaaa tgccttttta aaagaattga ggaagctgcg aaaacaggaa gcccaacaac
     1501 gacgaatgaa gcaatcgcac gaatcatcgc aagcctccga ggaccgttta aattagttga
     1561 gcttctagaa actttaaagt tccctaaggc aatctttatg tactggcaaa aacgattgga
     1621 ccgtaaaaat ccagatcaag agattgaagc ggaaatgctt a
//
LOCUS       DACEPD010000190         1628 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.200, whole genome shotgun sequence.
ACCESSION   DACEPD010000190 DACEPD010000000
VERSION     DACEPD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.200"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(150..1337)
                     /locus_tag="IW797_002780"
     CDS             complement(150..1337)
                     /locus_tag="IW797_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ1225514.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          491 a          337 c          279 g          521 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg
     1441 taatcaatga gacacaatta tggtttaaat ttgacccttc gaatagattt atcaaagatt
     1501 tttataaagt atggaattca gaagttttct ttttagcaat cgaagatagc ttattaatca
     1561 atctctacta ttctaataag aactatttca aaattcctgc tgcgaaaact agaatgaaga
     1621 aggatgta
//
LOCUS       DACEPD010000191         1578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.201, whole genome shotgun sequence.
ACCESSION   DACEPD010000191 DACEPD010000000
VERSION     DACEPD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.201"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..1297
                     /locus_tag="IW797_002781"
     CDS             128..1297
                     /locus_tag="IW797_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1225515.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          566 a          254 c          258 g          500 t
ORIGIN      
        1 atatcccata ttttaaaata aaagatagcg taaaatattt tgtgtaaata gaaatttagg
       61 gtagaaaaaa aagtccattc tgtaaaatta aagttaccac acagaaattt tataggagga
      121 ctttctcatg actaaattta ctatagaaat tatgcaaaca ctactcaata aaggggattt
      181 agatgaatta tttcaggatc atttagagcg agcgattaat tcacttctac aagcagagct
      241 aactgctttt ttagattacg aaaaatatga tcgaaatgga ttcaattcag gaaattcccg
      301 taacgggaat tattcacgta catttaaaac agaatatggc gagttgaatt taactattcc
      361 tagagatcgg aatggcgaat tttctcaaca aacactacca gcatataagc gaaccaatga
      421 ttcattagaa acgaccatta tccaattatt tcaaaaaggc attactatgg ctgaaatctc
      481 caaattaatt gaaaaaatgt atggtcacca ctatacgcca caaacaatct ccaacatgag
      541 taaattggtg gctgaagatg ttttagcttt taaagaaaga acgttagaag ccaattattc
      601 cgttatattt atggatgcga cgcatatccc tgtgaagcga cagactgttt cgaaggaagc
      661 agtgtatatt acgattggta ttcgtttgga tggaaccaaa gaagttcttg ggtttactat
      721 tgccccaact gagtctgctt atatttggaa agaagttctt caagatctta gaaaacgtgg
      781 gttagaagaa gttttattag tagtgacaga cggattaagc tgtattgaag aaagtatcca
      841 tagtgtgtat ccgaatgccc aatttcaaca atgttgtgtg catgtatcta gaaatatcgc
      901 tcataaagtt cgtgttcgag gtcgaaaaga aatttgtgag gatttcaaat tggtttacca
      961 agcgaattca aaagaagagg cattggatca catcgacttt atgactagga aatggaaaaa
     1021 gcagtatcca agagtcgtca atttactctt gaatcctgcc ctattaacct tttataattt
     1081 ccctcacgcc atcagacgaa caatttattc gacgaacctg attgaaggct ttaataagca
     1141 gctaaaacga tatactcgaa gaaaagaaca attccctaat gaagaatctc tagagagatt
     1201 cttcatttct caatttaatc aatataacca aaaattttta ggtagaattc acaagggatt
     1261 taaagaaatt caggatacat tagagtcgat gatttaactg taattaacag aatggatttt
     1321 ccatttacat ataattcttg acgctaccat aatctaaata accatcatat tcatctacta
     1381 cttatataat tgagttttat taaacttttt aagtttctat tctatcaaat atttaaacaa
     1441 tctctgcatc cgaaaagact acctcgtata gttttactaa caatattttt attagcgtgt
     1501 tcaaatcctg atttcttgta tcaattattt tctgttcact ttcaataaga aaaaagtctt
     1561 tacttccatg gataataa
//
LOCUS       DACEPD010000192         1553 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.202, whole genome shotgun sequence.
ACCESSION   DACEPD010000192 DACEPD010000000
VERSION     DACEPD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.202"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(521..1135)
                     /locus_tag="IW797_002782"
     CDS             complement(521..1135)
                     /locus_tag="IW797_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ1225516.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          515 a          291 c          247 g          500 t
ORIGIN      
        1 attttacaat cattgaaaaa ttgaatttca ttgcaaaaaa aataataaac acttataaat
       61 atccattatc tatccttcaa tttttgtgta ttttatttct attagaaaaa ggtatcctgt
      121 tggacaattt tgttatatca ggtaaacttc attgcaataa tgataacaaa tgtactataa
      181 ttactttgta aagagttttg gggaagactc tttcatcaac gtgtattaag agacaagaaa
      241 gaatccatat ggattctttc ttgtctcttt ttccactact ttttattttt ctgacactac
      301 atattcttat aactcatttc tcataactgt gaaacaactt ataaaaataa caaactttaa
      361 acaaaacaag cttaaaatca aagatgatcc atcttaaaat aactaaaaca aaaaaagtag
      421 tcgttgttaa tcctcattaa taatcgtttt ctttttttca aacgctccat ttttatgtgg
      481 aacattattt ttttaaggaa ctttgtcaac atgtggataa ctattttcca cttgtgtcct
      541 ggactagctg ttttgctgta aaatacaaaa cttgctgcag caaaaagatc gttgcaagag
      601 aggctgttcc gcagatcgct ggatctagat ttggcaaagt caagactgca tcatgtaaat
      661 tggcgtggtt gtccaaaacc aaatcagcaa attcaaataa tcgcaaacca ctgctatgtt
      721 gcgatttgat acttcgtgac aaatcaaatc cagtaacaat gattaatgga tgaccatttt
      781 ctttgatcca ctgagctagt tcgatgattg ccgggtttct tccttcattc gaaagaagaa
      841 gaaaaatgtc ttctgatttg acagaaagct gatcaataat tgcctgtcct gcaccttcta
      901 gcgtttcata gacacctccg gctggatttt caatcaatat acttggtaca agatccgggg
      961 tttgatccca aaattcaaat gctacaccac ttagtgtgcg gctagcatat atctgcaatc
     1021 taccaccttg attaatttgt ttcgcgatcc aagtgccagc ttgaagcatc agcggctctt
     1081 ctatccttaa tttctcttcc aattggtgat aatagtcaaa ataacataaa ctcatttatc
     1141 tctcccgcct agctatactt gacatgaata gaaacagctg cctaatctaa ggaagtacat
     1201 aaaaaaacag tagttagaca gtaacacatg tggtctttgc tgtttcaaac acaaaaaagg
     1261 cattgaacga atacgcccaa taccttgaca tagcaaataa aacaacgtgc agaccatgga
     1321 aaatatctac ataggggtag aattttcacc ctagattagg tacaataaat taggtcgatg
     1381 cactgcattg actatggcaa aatgatcggt cagggtgtat gtgtgtgact ggtcattccc
     1441 tgattcttag cgccaactaa gggtcaggga gccttttttt attcagctgt ttctatctga
     1501 atttattgta agtaatctct tatccaagtg caagcaagaa aatacaaaaa aac
//
LOCUS       DACEPD010000193         1537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.203, whole genome shotgun sequence.
ACCESSION   DACEPD010000193 DACEPD010000000
VERSION     DACEPD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.203"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            27..1493
                     /locus_tag="IW797_002783"
     CDS             27..1493
                     /locus_tag="IW797_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ1225517.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          425 a          289 c          325 g          498 t
ORIGIN      
        1 aaatttaaag aattggggta tagattatgg aaaaacagca agacaagggc ttcttcgggc
       61 agccgaaggg attgtcaact ttgttcttca ctgagatgtg ggaacgattt agttattacg
      121 gtatgcgtgc attacttatc tattatatgt acgatacagt tgccaacggg ggacttggat
      181 taccacgagc aacagcatta gctatcatgt cgatctacgg atctttgatc tacatgtcta
      241 gtatcgtcgg cggatgggta tctgaccgag tgttaggagc aaaacgcact gtattcttcg
      301 gtggagtttt cattatgctt ggccacatcg tcttggcttt accatttggt gtaagtacat
      361 tgtttgtttc gatggcattg attattatcg gaaccgcctt tttgaaacca aatgtttctg
      421 gcatggtcgg acatctttat tcaaagactg acttgcgaag agatgcagga ttttcgattt
      481 tttacatggg tgtcaactta ggggcattat tagcaccact aatcgttggt acaatcggtc
      541 aagaatataa ctatcattta gggttctctt tagctgctat tgggatgttc tttggattat
      601 tacaatacat catccaagga cgcaagacat tagacggagt gggtacaaca ccgccgaatc
      661 ctttgacaac tgaagaacgt aaaggattca tccgtaactt gatcatcgca cttgttgtta
      721 tcgctgcagt attcggcggg gcacaactaa ctggtcatct gacaattgac ttttttgtca
      781 atacaatcag tgtattgggg attatcttac cgttgtatta ctttatcaaa atgctgactt
      841 caaaagatgt aactgctgaa gaaaaaccaa gagtatgggc ttatatccca ttattccttg
      901 cagcaatcat tttctggtca ttacaagaac aaggttcatc tatcttagct ttatttgcta
      961 gtgaacgtac acaatctacg ctatttggtg taacgatcca agcaagctgg tatcaatcat
     1021 tgaatccaat tttcatcttt attctgacac cagtattcgt aacgatgtgg acgaaacttg
     1081 gtgaacgtca accatctaca gtagtgaaat tctctcttgg acttttattt gcaggattat
     1141 cgtacttgct attgatgttg ccgggtatgc tttatggtac aagtggaaaa gtaagtccat
     1201 tgtggttgtt gggaagtttc ttcatcgtgg ttattgctga attgtgtctt tcaccagttg
     1261 gtttgtctgt gacaacaaaa ttagcaccac gagcatttga atcacaaaca atcgctattt
     1321 ggttcttagc tgatgcttct tcacaagcaa tcaatgcaca aattgcacgc ttctacacac
     1381 cgggaacaga atctgcttac ttcggtatta tcggtgcagt agcaatcgtc ggtggtatct
     1441 tgctatttac tgtgaaagaa ccaatcaaaa aactaatggg ctctattcat taatcaatga
     1501 acataaaaac aggaattgct tatttgcagc agttcct
//
LOCUS       DACEPD010000194         1515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.204, whole genome shotgun sequence.
ACCESSION   DACEPD010000194 DACEPD010000000
VERSION     DACEPD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.204"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            287..694
                     /locus_tag="IW797_002784"
     CDS             287..694
                     /locus_tag="IW797_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225518.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IW797_002785"
     CDS             708..1109
                     /locus_tag="IW797_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225519.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IW797_002786"
     CDS             1111..1482
                     /locus_tag="IW797_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225520.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          575 a          185 c          264 g          491 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt ttttt
//
LOCUS       DACEPD010000195         1493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.205, whole genome shotgun sequence.
ACCESSION   DACEPD010000195 DACEPD010000000
VERSION     DACEPD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.205"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(135..542)
                     /locus_tag="IW797_002787"
     CDS             complement(135..542)
                     /locus_tag="IW797_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225521.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(561..1379)
                     /locus_tag="IW797_002788"
     CDS             complement(561..1379)
                     /locus_tag="IW797_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ1225522.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT          446 a          242 c          205 g          600 t
ORIGIN      
        1 ataatgttga tttaatcgca aatttacacg ttgtttatta agttatacct taaatattta
       61 atgtataact tatactttaa tattcattta tctagtataa cttatacttt aattgaatac
      121 cgcatgaaaa aaaattattt tggtaatttt tcaaactctt cttgtatttc ttctcttatg
      181 tgttcttctt ttgctactcc tagtctatgc tggatatatg agtctgctaa tgtattaaca
      241 attttttcga aagaaattct ccctttttca ctttgtaact ccgtcatata gtcttgtaaa
      301 attgagagtt tgctatttaa tgccggagag atctttttag gaactgatac cttgtttctt
      361 ttatcaggaa ttgaaactgg tcggcctctt tttttcaatg taatatttct tgtgttactc
      421 aatgcactat tgctaaagtt atattcaccg cttattttca catcttggcg tttgttattg
      481 ggtaataatg gctcaaattc accattctcc atcattttag gaatatcaaa atctttaggc
      541 attacttatg caactcctta ttatttattt aaaaactctt gtctagcatc tagttctgcc
      601 aaaatattta taaatacttg gtgtgcttta taatcccatt gatcaaaatt tccatttttg
      661 tattttctta aatgtatacc aattttagaa taccttctca atcttttttg ataatgaatg
      721 agatttttgg ataccacggt tccgtatttt tcaattgctt cttctaggat ttcttcatca
      781 aaatcatcat ttggttctac catacaaggt acaatatcta taatttgcaa atcaagatcg
      841 tatctatcga ccatgaattt ttgataattt acatatttgt tgactccctc taaggattct
      901 tcagctgttt gaaatgaaat tatcgaatag tctgaagctg ccatagcatt atcggaaaat
      961 tcgctaatgg ttggtggtac atcaataaat atttcttgat aattgttctt tattggaaga
     1021 agtaattttt tcaagacagt aacttggtct aattcatttt catgtttatt agcaaaagcg
     1081 gagaatgatc gaaaactagt gtcacatgcc agaaggtcaa gattttcatt taccttaata
     1141 atactacttg ctaattctcc atttgcgaca ccatttgtta tagtggtttt gatatcggtt
     1201 ataccgaaag ttcttgctat gacttgtgtt aagttcgctt gagggtctaa atcaataata
     1261 agcgtttttc tatttcttat cattgcattg tcataagcaa gcatgctgac tatcttagtt
     1321 ttacctactc ctcctttaaa attgccaact gtaaatgtag tcccttgaaa agtatccatg
     1381 taaatcctcc caatctttaa ttgataatta aagtataact tatatatcgg aaatggtcaa
     1441 cattaaaagt ataacttaaa taataatatc taaaaacgtt tggatatagc ggt
//
LOCUS       DACEPD010000196         1457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.206, whole genome shotgun sequence.
ACCESSION   DACEPD010000196 DACEPD010000000
VERSION     DACEPD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.206"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            109..1041
                     /locus_tag="IW797_002789"
     CDS             109..1041
                     /locus_tag="IW797_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ1225523.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1113..>1457
                     /locus_tag="IW797_002790"
     CDS             1113..>1457
                     /locus_tag="IW797_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225524.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          228 g          420 t
ORIGIN      
        1 gatgaaataa atcaaaagta tgatgaaaca ttaaaaaatg tctattcttc acttatcgtt
       61 tcaaatatag aagaaacttc atcttctgaa ataaaaaata agctttcttt gttaaaatta
      121 aaagaaaagg aatatattat tgacgatgct gtttctgtcc ctcttatttt aaataaacga
      181 gagaaaaata atttaaagaa aaaaatgctc attccatttg attcagaagt gaaaggatta
      241 ctcaataaac taactatttc taatatagac tcgactaggg aaaaaatcat aaaaatattt
      301 gataaaatta ataatttatt caagaatgac aatgctgaat taaggaaggg tcttttagag
      361 tttaaacttc aagaactaga acttcattac tcttatttag tgaaaattac cgaagaaaaa
      421 gaacaacaaa aagcaataaa agaacagatg atagaagagg aaaaagttcg tagagaaatt
      481 gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat tcaattctga aatttccaaa
      541 ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa aagaattata tgcaaataaa
      601 attcaagaac tggaagaaaa attaaaagaa ttagaagtta tacgtgaaaa tgtattacaa
      661 agagaattaa atactagagc cggttatgtg tatgtaatta gtaatattgg gtcttttggt
      721 gaagacatat ataaaattgg tatgactaga agattagaac ctatggatag aataaaagaa
      781 ttgagtagtg catcggttcc atttgaattt gacgttcatg cgatgatttt ttcagaggac
      841 gctccgtctt tggaaactaa attgcataat cattttagaa aacaagaagt taataaaatt
      901 aatcaaagaa aagaattttt caaagtttca ctagacgaaa tagagaaagt tgttttagaa
      961 aattataatg gaacagtaac atttacaaaa ttagctaaag ctgaacaata tagacgatct
     1021 ttagaattgt caaaagatta aatatataaa aagccttcgg gctttttctt ttaacaatta
     1081 ataggaacgt atattcgaaa ggaatagaaa taatgtggtt agaaaaaata aatgacaaaa
     1141 aatttaagta caccgaacgt tatacggatc ccttaactga aaaaaagaga aaagtgtccg
     1201 ttactttatc tagcaattca cgccaagctt ggaatcaagc taacttactt ttgaatgaaa
     1261 aaatcgcaga aaaaataaaa cgaaatgaag aattaccact aacttttgga gaattaaaaa
     1321 ctaaatggga tgaaaaatac aagccaactg taaaagaaag ttcctatcga acaactcagg
     1381 tttatctatc attaatttca aaatatataa aagatgatgt acttgtaaaa aacgttaata
     1441 gtaatcttat acaagat
//
LOCUS       DACEPD010000197         1444 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.207, whole genome shotgun sequence.
ACCESSION   DACEPD010000197 DACEPD010000000
VERSION     DACEPD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.207"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..226
                     /locus_tag="IW797_002791"
     CDS             <1..226
                     /locus_tag="IW797_002791"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ1225525.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            208..513
                     /gene="kduI"
                     /locus_tag="IW797_002792"
                     /pseudo
     CDS             208..513
                     /gene="kduI"
                     /locus_tag="IW797_002792"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            533..1335
                     /locus_tag="IW797_002793"
                     /pseudo
     CDS             533..1335
                     /locus_tag="IW797_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
BASE COUNT          462 a          259 c          344 g          379 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgcgcg
     1021 gcaaaaggcg gattaaaaat gctgacgaaa aacatcgctt ctgaatacgg tcaatacaat
     1081 atccaatgta atggaattgg accaggttat attgctacac cgcaaactgc tccattacgc
     1141 gaaacacaag aaaacggtga acgtcatcca ttcgatcaat ttatcgttgg acgtacgcca
     1201 gcagcacgct ggggcgagcc aatcgatttg gctggaccat ctgtcttctt ggcttctagc
     1261 gcatctgatt tcgttaacgg ccatgtcttg tatgtggacg gaggcatact tgcctatatc
     1321 ggaaaacaac cttaaaagag ctttagtaca ccgtttagtg gagaggttgt gaaatcagcg
     1381 gcttgaacga ataccgaagg acagctgatt aaacatcatt tattcgaaaa aagaggctgg
     1441 gaca
//
LOCUS       DACEPD010000198         1371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.208, whole genome shotgun sequence.
ACCESSION   DACEPD010000198 DACEPD010000000
VERSION     DACEPD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.208"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            72..1367
                     /locus_tag="IW797_002794"
     CDS             72..1367
                     /locus_tag="IW797_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ1225526.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          437 a          271 c          260 g          403 t
ORIGIN      
        1 aaaaaatata gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag
       61 gaggattcaa gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa
      121 atatctattt cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga
      181 tttacaaagg aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc
      241 cctctaggat cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg
      301 agtatcgcac ttatctggag ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa
      361 cattcgtagc ggatacctct gttgctgaaa aacactgttt cattagtcaa aaagttcgtt
      421 ggtctgtcat tgctcgctta aaagagaata cttccatgac ggaaatcgct cgtcagaaaa
      481 atatttccac ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt
      541 tcaaacagac tttgccaaag gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg
      601 ccagtgcaat gagttttatc atgatggacg gacaaagcca tgcattattg gatattgtcg
      661 aaaatcgacg cttgccttat ttagaacgct atttttctcg gttttcatta gcgactcgtg
      721 aagccgttca attgattgtt attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc
      781 ttttcccgaa tgctcagttg atcatcgatc gtttccacat cgtccaacat attggcagaa
      841 ctttcttgaa tcatcgagtg aaagaaacca cggtacgact taaaggaaat tcccactctc
      901 aacgagggtt agggaaaaaa ttgaagcgct attggaaact cctacaaaaa gaagaagcaa
      961 agttaaacta tgaaaaacgg gtttggcgtg ccagttttaa agattatttg actgaaagtg
     1021 agattgccga tcgactgtta gctgcttgtc ccaacttacg ccaaggctat cagttatacc
     1081 aagacattct ttatgcggta aagaaaagag atcagtcact atttgaggat tgtttaacta
     1141 gagacattag cggtctgcca gaaacctata caaccacttt acgaacgttt aaaaagtttc
     1201 tgccacagat ccaaaatgcg cttcactaca gctattcaaa cggaccattg gaatgcttaa
     1261 acaaccacat caaagtatta aaacgcaatg cgtacggctt tcgcaacttt tacaatttca
     1321 aactacgaat tatgattcgt catggcaaaa catttttaac caaataaaca g
//
LOCUS       DACEPD010000199         1335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.209, whole genome shotgun sequence.
ACCESSION   DACEPD010000199 DACEPD010000000
VERSION     DACEPD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.209"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..1285
                     /locus_tag="IW797_002795"
     CDS             <1..1285
                     /locus_tag="IW797_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ1225527.1"
                     /translation="RVVGTKADKIKHLPITEKEHLLPLEEQFCEHCGSQMKDIGRTKV
                     REEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETI
                     RMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEI
                     LHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYL
                     HCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKK
                     WKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFE
                     DGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYL
                     EFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          442 a          241 c          285 g          367 t
ORIGIN      
        1 acgagttgta ggaacgaaag cagacaaaat caaacactta cctataacag agaaagaaca
       61 tctccttcct ttggaagaac aattttgtga acattgcggt tcacagatga aagatatcgg
      121 acggacaaaa gtacgagaag aaattcgttt ccatcaggct atgttggatt gtctcaccca
      181 ttatcaacat acctattgtt gtaaaagctg cgaaaaagaa ggactttctt cttttaaaaa
      241 agcgatcgtt cctaaacctt tgatttctaa tagcttaggt tccaatagtt tagtggcgga
      301 aacgatccgg atgaaatttg gtcagaaagt tcctgcctat cggcaagaga actattggaa
      361 acaaacacat gggttggata tttcgagaga taatatcacc aattggcata ttaaagcagt
      421 gcaaaacgcg ttagaccctt taggagaacg attaagagtc tatcttaatc aagaagagat
      481 tctacacggg gatgaaacaa gttatcgtgt gattgaaagt gccaaaacag atacctatta
      541 ctggcagttt tgtacaggaa aagacagtca gcatcccatc gtttattatc accacgacga
      601 aagtcgtgct ggagatgttc cgaaaacttt tctcaaggag tttacagggt atttacactg
      661 tgacggctat agcgggtata atgccgtgga aagtgtacga cttgtctatt gtttcgcgca
      721 tgttcggcgg aaatttttcg aagcaattcc aaaaggaaag aaaaacacgg atattcccgc
      781 agctcaagca gtcaaacaat tggataaatg gtttgtccta gaaaagaaat ggaaagactt
      841 ttctcctgag aagcgattaa gctgtcggca acaagaactg cgtccattat ttattgcttt
      901 ttatgagtgg atggcaacga tagatcctgt tgcaaaatcc aagttggatg cagccgttca
      961 atatgcgtgt aaactacgaa gtggttttga acctattttt gaagatggtc gtttagaact
     1021 cacaaataat cgtgcggagc gaaatatcaa agagctggtg ataggacgga aaaattggtt
     1081 gcattccaca agtcttgaag gagcacgaac ttcaggaatc attctgagtg tatataaaac
     1141 agcagaactt aacggattga atcctgtaaa gtatctagag tttctatttg ataagatacc
     1201 aaatcttccc gtactttcag cagaaacttt agatcaactg ttgccttgga acaaggatgt
     1261 acaacaacat ttttctcgta actaaaaacg ccaaacattc ccgattctat tctataggtc
     1321 gggttatttg acgta
//
LOCUS       DACEPD010000200         1276 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.210, whole genome shotgun sequence.
ACCESSION   DACEPD010000200 DACEPD010000000
VERSION     DACEPD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.210"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            35..532
                     /locus_tag="IW797_002796"
     CDS             35..532
                     /locus_tag="IW797_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ1225528.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..1252)
                     /locus_tag="IW797_002797"
     CDS             complement(569..1252)
                     /locus_tag="IW797_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225529.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          444 a          216 c          224 g          392 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgccc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat tttgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaa
//
LOCUS       DACEPD010000201         1271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.211, whole genome shotgun sequence.
ACCESSION   DACEPD010000201 DACEPD010000000
VERSION     DACEPD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.211"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            130..1215
                     /locus_tag="IW797_002798"
     CDS             130..1215
                     /locus_tag="IW797_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ1225530.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
BASE COUNT          530 a          187 c          200 g          354 t
ORIGIN      
        1 acttattttt ttaatatata acttatacct taaatactga aagtgtttta ttcaccctta
       61 atacatggta tagttcactt gttgaaaatc ctgaatatat acaagtttct aagaagaagg
      121 agaaatttaa tgtctacaga aaatcaatct caaaaaatga taattaaaaa ttggggatat
      181 aaagcaacga atgtttatat tgaaaaatat tataaatttc ctagtttttt aattcagaat
      241 tctaaatact ctaaaatgaa agacagctca aaaatagcct atatgctttt taaaaatgaa
      301 tttcgtagag ccttacatga aaaatggatc gacgatgatg gtctattata tttggaattt
      361 acacaagcag acctaatgga caaattaaat tgctaccaag gcaaagtaaa aaccatttta
      421 aacgagttag aaacatataa tttgatttca gtcgtaaaag gagaatttaa cccaaaaacg
      481 aaaaaaaatg aaaaaaataa atactattta cttcaacctg aaataagtgc cgatgatctt
      541 tttattcaaa attctttcga tgacgattta agcgaaacta ttgataaatc aacctcttct
      601 cggaatgcgg aaatcgcacg tcgagaggaa actgctaaaa cccttgataa cactggaaat
      661 gcggaaatcg cacgtcgaga aaaaacagct gaaacccttg ataacactgg aaatgcggaa
      721 atcgcacaag attattatat taataaatct ttagacacta aaagacactt aaaagacact
      781 gaaacagacc aattacaaaa tcaagttttg ctagataact tcgttgaaat catgaaagaa
      841 gacagcataa acacatttgt accagaaaag gtattaagct tgataaaaac attttctaac
      901 acttacgaag aagcccaaca aaccgtcacc acaatacaca aagcaaaaca taaagccgag
      961 gaacttgaga agacggtagt tgtgtatgaa gaacttgaat cttacggaat tgatgcggat
     1021 aaaggattat atatgacgtt gctgaaagct tatcaaaaac aaaagactga gaaagtaaat
     1081 aatttgcaga atttgatttt tgtgtatgta aaaaactggt ttattgaaaa agctattgcg
     1141 gcaaagcttg caaacgaaca aagagaaaca tttaacgaat taccaacagt tagtacggaa
     1201 aattggctag agtagttgat aaaaagcgat cactataaaa atgatcaatt ttaaattaga
     1261 acttttttat g
//
LOCUS       DACEPD010000202         1248 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.212, whole genome shotgun sequence.
ACCESSION   DACEPD010000202 DACEPD010000000
VERSION     DACEPD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.212"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            200..1248
                     /locus_tag="IW797_002799"
                     /pseudo
     CDS             200..1248
                     /locus_tag="IW797_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          516 a          141 c          236 g          355 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattaa aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagat
//
LOCUS       DACEPD010000203         1231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.213, whole genome shotgun sequence.
ACCESSION   DACEPD010000203 DACEPD010000000
VERSION     DACEPD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.213"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>1231
                     /locus_tag="IW797_002800"
     rRNA            <1..>1231
                     /locus_tag="IW797_002800"
                     /product="23S ribosomal RNA"
BASE COUNT          323 a          280 c          375 g          253 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc c
//
LOCUS       DACEPD010000204         1215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.214, whole genome shotgun sequence.
ACCESSION   DACEPD010000204 DACEPD010000000
VERSION     DACEPD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.214"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            414..947
                     /locus_tag="IW797_002801"
     CDS             414..947
                     /locus_tag="IW797_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ1225531.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACEPD010000205         1157 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.215, whole genome shotgun sequence.
ACCESSION   DACEPD010000205 DACEPD010000000
VERSION     DACEPD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.215"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(21..94)
                     /locus_tag="IW797_002802"
     tRNA            complement(21..94)
                     /locus_tag="IW797_002802"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="IW797_002803"
     tRNA            complement(108..181)
                     /locus_tag="IW797_002803"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="IW797_002804"
     tRNA            complement(209..281)
                     /locus_tag="IW797_002804"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="IW797_002805"
     tRNA            complement(301..374)
                     /locus_tag="IW797_002805"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="IW797_002806"
     tRNA            complement(384..457)
                     /locus_tag="IW797_002806"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="IW797_002807"
     tRNA            complement(468..553)
                     /locus_tag="IW797_002807"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="IW797_002808"
     tRNA            complement(570..641)
                     /locus_tag="IW797_002808"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="IW797_002809"
     tRNA            complement(653..725)
                     /locus_tag="IW797_002809"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="IW797_002810"
     tRNA            complement(743..824)
                     /locus_tag="IW797_002810"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="IW797_002811"
     tRNA            complement(832..904)
                     /locus_tag="IW797_002811"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="IW797_002812"
     tRNA            complement(908..980)
                     /locus_tag="IW797_002812"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
     gene            complement(994..1109)
                     /gene="rrf"
                     /locus_tag="IW797_002813"
     rRNA            complement(994..1109)
                     /gene="rrf"
                     /locus_tag="IW797_002813"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          299 a          326 c          270 g          262 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact taagcgtggc gacgtcctac tctcacaagg
     1021 ggcaacccct cactacaatc ggcgctaaga agcttaactt ctgtgttcgg catggttaca
     1081 ggtgtatcct tctcgctatc gccaccacac tgtggtgtta tcttttattg agtaaatttt
     1141 gttcactcaa aactgga
//
LOCUS       DACEPD010000206         1103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.216, whole genome shotgun sequence.
ACCESSION   DACEPD010000206 DACEPD010000000
VERSION     DACEPD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.216"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..969
                     /locus_tag="IW797_002814"
     CDS             <1..969
                     /locus_tag="IW797_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1225532.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          381 a          193 c          220 g          309 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggt
//
LOCUS       DACEPD010000207         1073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.217, whole genome shotgun sequence.
ACCESSION   DACEPD010000207 DACEPD010000000
VERSION     DACEPD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.217"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            2..>1073
                     /locus_tag="IW797_002815"
     rRNA            2..>1073
                     /locus_tag="IW797_002815"
                     /product="23S ribosomal RNA"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DACEPD010000208         1062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.218, whole genome shotgun sequence.
ACCESSION   DACEPD010000208 DACEPD010000000
VERSION     DACEPD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.218"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(94..435)
                     /locus_tag="IW797_002816"
     CDS             complement(94..435)
                     /locus_tag="IW797_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ1225533.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
BASE COUNT          313 a          247 c          130 g          372 t
ORIGIN      
        1 attccttact caatgcctaa ggtaatacta cctttttatt aatcatcgtg actccttcta
       61 agcctttatc tgtaaaattt attcctccag tttttattct tctttatccg ctacaaattc
      121 aatgatttca acgctgacaa tataatcact caaatatgac caagtatctt caacagataa
      181 ttcgtctcca ttatcatccg taagcgtcat tttaaaccat tcattacctt catcatcttc
      241 aggtagcaag ccgaatccaa ttggattatc agtcaatgtt tgatcgtata aatcatcctc
      301 gtctaatgct aaaacatcta atcctttaca atttccgcct cttctaaacg tcacaaatcc
      361 tttgtaatcc caacgttgga aagaaacctt aatagtatgt gtgcccaatc gtatattcgg
      421 atcataattt ttcatttgtc attcctccat tacgtaaatt tcattttctc tatgttttat
      481 atcaattttt tcactttatt caggacttgt tcatatttta tcgtttttcg tttacttaaa
      541 aatacataaa aaatttcgac tcgtttatgg cgtccgaaac gagaaaccct agtagacagc
      601 ggtaaatgct cgattgatcc aataatcaac gtcctcgcaa gtatctacat gtactgcagc
      661 acacgacaca atttcttgtg cgtcctcccc attacgagaa gacctactcc ttattccatg
      721 agtttgcgtc aaagtctaga tcccttagta gctgctcatg attaatctag acgctattct
      781 aacttgtcta acttctaaat tccctcttgt ttactctgcg cttattattt acacaaattc
      841 atttcaccac tatccaaaca agcgcattta ctcgattgac caatttttta cgttaccaat
      901 cgttaaaacc taccccgtca ctccccgaag ggaattgttg atagatcaag tctactcctt
      961 atttttctag tgactctaag ttcctttaga agtctcgaca atattttcaa gcaagcgaaa
     1021 aacgataaaa tacaagttat gagaatgatc aattcagccg at
//
LOCUS       DACEPD010000209         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.219, whole genome shotgun sequence.
ACCESSION   DACEPD010000209 DACEPD010000000
VERSION     DACEPD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.219"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>1035)
                     /locus_tag="IW797_002817"
     rRNA            complement(<1..>1035)
                     /locus_tag="IW797_002817"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACEPD010000210          978 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.220, whole genome shotgun sequence.
ACCESSION   DACEPD010000210 DACEPD010000000
VERSION     DACEPD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.220"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            82..>978
                     /locus_tag="IW797_002818"
     CDS             82..>978
                     /locus_tag="IW797_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ1225534.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYE"
BASE COUNT          333 a          182 c          192 g          271 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaa
//
LOCUS       DACEPD010000211          935 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.221, whole genome shotgun sequence.
ACCESSION   DACEPD010000211 DACEPD010000000
VERSION     DACEPD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.221"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..818
                     /locus_tag="IW797_002819"
     CDS             <1..818
                     /locus_tag="IW797_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ1225535.1"
                     /translation="TRYQLIKQLIRTKQHFLENLTYKCNTLAREMRDESTSLFSATLI
                     SLMTEDFTLDELAELPLEAFCDLLQEKGKGRFKQPEKIAKAIQRAITMSYRLGALAQE
                     SINVVLSVLVREIRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEI
                     GQIERFEDQTKLAKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYL
                     PEYKAFYQSKYKEVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          334 a          182 c          163 g          256 t
ORIGIN      
        1 gtactcgcta tcaacttatt aagcaattga ttcgtacaaa gcaacatttt cttgaaaatc
       61 taacgtacaa atgtaatacg cttgctcgag aaatgcgaga cgaatcgacg agtcttttta
      121 gtgcaaccct catttcactc atgacggaag atttcacatt ggatgaactt gctgagcttc
      181 ctttagaagc tttctgtgat ttacttcaag aaaaaggaaa aggccgtttc aagcaaccag
      241 aaaaaatcgc taaagcaatt caacgtgcaa ttaccatgag ttaccgccta ggtgctcttg
      301 cacaagaatc aattaatgtt gttttaagtg ttctcgtacg agaaatacga gcacttgaaa
      361 aaaatatcaa agaattagat aaagccatcg aacaagtaat tgttgtcatt ccagaatatc
      421 aatgcttaac cagtattcca ggcgttggta aagtctatgc tgccggttta atcgctgaaa
      481 ttggtcaaat cgaaagattt gaagatcaga caaaattggc aaaatacgct ggattaagtt
      541 ggaaagtgaa ccaatctgga aactatcagt cgcaaaatac acctctgaca aaacaaggaa
      601 atcgatattt tagatactac ttagttgaag ctgccaactc tgtaaaaaat tatcttcctg
      661 aatacaaagc tttttatcaa agtaaatata aggaggttcc aaagcatcaa cacaaacgtg
      721 cactcgtcct aaccgcaaga aaatttacgc gtctggtgga tacgctacta cgtaaacacc
      781 aactctatac gccaccaagg agtgtgatag agaaataaca atccgttatt gacgcaaatc
      841 cttgaatcaa cccgaaaaaa atttgatttt gatcgggtct agtttcgtgc gcttcaaaac
      901 atattactca ataaagaaaa tcttaaattt catct
//
LOCUS       DACEPD010000212          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.222, whole genome shotgun sequence.
ACCESSION   DACEPD010000212 DACEPD010000000
VERSION     DACEPD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.222"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..888
                     /locus_tag="IW797_002820"
     CDS             <1..888
                     /locus_tag="IW797_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ1225536.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACEPD010000213          883 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.223, whole genome shotgun sequence.
ACCESSION   DACEPD010000213 DACEPD010000000
VERSION     DACEPD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.223"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            93..875
                     /locus_tag="IW797_002821"
                     /pseudo
     CDS             93..875
                     /locus_tag="IW797_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT          371 a          124 c          116 g          272 t
ORIGIN      
        1 acaggtaacc gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga
       61 caatttattt acacaaaatt attgacgctc cccaagatat gctagattac tactattttg
      121 aaaaaaattt atcatataat tatgtaatac atttaaaaac ttttacagga atgattttta
      181 aattcgccaa ccgtcgatat ggattaaaat ataatcccgt aaatggggtt tctttagcaa
      241 gaaaacctaa gacaaacgaa gaaataaaaa aagaaaaaga tggttattta tcaaaagaag
      301 aagttcaaat gattgctagt aaacaaaaaa gttcacatca acagtcacgt tattctttaa
      361 ttactaaatt tttgttttta actggactac gttatggtga attgatatct ttgactgaat
      421 ctgattatga tggtaataaa attacggttt ctggtactta tgattacgag ttaaaaatta
      481 aaacaacaac taaaaataca ggttcttata gaaccattga attatcaagt aatgctaaag
      541 aaatcataga tcaattaatt gaagaaaaca aattaattaa gaataataaa gataaataca
      601 ttttcatctc taaaaatggt aacccgatat ctattcaagc atataatcaa tctttaagag
      661 cagtatccaa agaattaaac atagataaaa aagtttcttc tcacatgtta aggcattccc
      721 acatttctct acttacagaa ttaggaatcc cattaaaagc cattatggat cgtgtaggac
      781 atgaagattc aaaaacaaca ttaaagattt atactcatac aactaaaaat atgcaaaatc
      841 aactagtaga aaaactagga aaaatagaaa tttaaattgc ccc
//
LOCUS       DACEPD010000214          823 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.224, whole genome shotgun sequence.
ACCESSION   DACEPD010000214 DACEPD010000000
VERSION     DACEPD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.224"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            22..>823
                     /locus_tag="IW797_002822"
     CDS             22..>823
                     /locus_tag="IW797_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1225537.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT          253 a          142 c          186 g          242 t
ORIGIN      
        1 cgaggagggt ggaaagaaac cttgccagaa attgcggatg aaatcagtat cctcaacttg
       61 acgagtaagt cagaaaatca aggtctgctt gatccttatg tgattatgaa gaaaaagaaa
      121 gactcggaaa gtttagcaat tgatattctg acttttttaa caggaatttc aagtcgagac
      181 ggggagaagt ttcctgtgtt acgtaaagcc attcgaacgg tgactcaaag tgatcaacgg
      241 gggttattac aagttattaa cgaattgcga aaagacgggt caccaatcgc ggaaaatatc
      301 gccgatcata tcgagtcctt taccgattat gattttgctc atttgctttt ctcagacggc
      361 tcgattaccc aatcgattag tttagataaa cagctaaata tcattcaagt ggcggatttg
      421 gttttaccgg atgcagaaac gacttttgaa gaatatacca ccatggaatt gcttagtgtt
      481 tcgatgctaa tcgtcatttc cacttttgcc ttggacttta tccatagtaa ccgagaaatc
      541 ttcaaaattg tcgatttgga tgaggcttgg agttttcttc aagtggctca aggaaaaacg
      601 ttatcgaata agcttgttcg tgctggtcgg gccatgaatg ctggggtcta tttcgtcact
      661 caaaatagcg acgatttatt agatgaacgt ttaaaaaata atatcgggtt gaagtttgct
      721 tttcgcagta ccgatattca tgaaatcaag aaaaccttgg aattctttgg tttggatcag
      781 gaggatcaaa gcaatcaaaa acgcctgcga gatttggaaa acg
//
LOCUS       DACEPD010000215          791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.225, whole genome shotgun sequence.
ACCESSION   DACEPD010000215 DACEPD010000000
VERSION     DACEPD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.225"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            24..716
                     /locus_tag="IW797_002823"
     CDS             24..716
                     /locus_tag="IW797_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ1225538.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          199 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttatttt t
//
LOCUS       DACEPD010000216          775 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.226, whole genome shotgun sequence.
ACCESSION   DACEPD010000216 DACEPD010000000
VERSION     DACEPD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.226"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(249..773)
                     /locus_tag="IW797_002824"
     CDS             complement(249..773)
                     /locus_tag="IW797_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225539.1"
                     /translation="MIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
BASE COUNT          262 a          106 c           87 g          320 t
ORIGIN      
        1 tgacgctccc ttaacaataa atgaagactt ttttattgtt aattgtttat ttttctatta
       61 ttagatatga aaatcgcatt tagataaaat agaaaaatat gaatatattt taaatattaa
      121 gatgagagtc attaacttat gatagtagtc aatttgcttt tcactaatta caaaatatgt
      181 gatataggaa gtttagttat tgttctcata atgccatcct gtttttagat tatctagtaa
      241 tttttttgtt atttaaaatt aggagcagtg ccaattgttt taattttaaa ggcttttttt
      301 atatcttcta aattagaaga tatttgtata tcaatatgga atatattacc ttttttgtat
      361 ttttttatgt tatctttagc taaaacatca aatacatgtc cttgaaattg tatcgaggca
      421 gctggattta gtttaaattg aggaaacttt accacagctg attcaaaaca cttgtcacat
      481 ttttttattt ttttaccttt gtaaaaatat tcgattgaat attctatttg gcacgactca
      541 cttggcagta tatgtatgtc aatatttttc atctgtttca taggtatttc aaaatctaaa
      601 ctattaaaaa catataattc cctgtaatta tttacatcta actctactaa atttatcttt
      661 ggaacaatag atcctccacc cattccacca taataaaaaa aatcttgttc tctatcaaaa
      721 tagcacatat taagctcagt ttgtttatca tcctcaataa ttttataaat caaac
//
LOCUS       DACEPD010000217          772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.227, whole genome shotgun sequence.
ACCESSION   DACEPD010000217 DACEPD010000000
VERSION     DACEPD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.227"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            95..>772
                     /locus_tag="IW797_002825"
     CDS             95..>772
                     /locus_tag="IW797_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ1225540.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGE"
BASE COUNT          293 a          128 c          153 g          198 t
ORIGIN      
        1 aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag aagtttggaa
       61 atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga taaatcgaag
      121 taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt ttccatcgta
      181 taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa
      241 ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca tatttacgta
      301 tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat cctataagaa
      361 cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc aggaaattcg
      421 atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca atgtcatacg
      481 ggacgggata gaacaaacaa tagatataga agaccttgta atagatgaca ttgttatttt
      541 agagactgga catcaaattc cagcagatgc agaggtcgtg gaaggaaaac tgcaagttaa
      601 tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggcg atccattaat
      661 gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag ttggagcaga
      721 ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag aa
//
LOCUS       DACEPD010000218          751 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.228, whole genome shotgun sequence.
ACCESSION   DACEPD010000218 DACEPD010000000
VERSION     DACEPD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.228"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            362..730
                     /locus_tag="IW797_002826"
     CDS             362..730
                     /locus_tag="IW797_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ1225541.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT          243 a          117 c          157 g          234 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt caaaaaatag
       61 cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga gaacaccgtt
      121 taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt ccatataaat
      181 acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa cttatttatt
      241 attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta gattttccca
      301 ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta ggaggaaaca
      361 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
      421 gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg ggacgcttat
      481 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
      541 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      601 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      661 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      721 aggtaaataa tagaaataaa gactttgttt t
//
LOCUS       DACEPD010000219          747 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.229, whole genome shotgun sequence.
ACCESSION   DACEPD010000219 DACEPD010000000
VERSION     DACEPD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.229"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            553..>747
                     /locus_tag="IW797_002827"
     CDS             553..>747
                     /locus_tag="IW797_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1225542.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYY"
BASE COUNT          271 a          136 c          114 g          226 t
ORIGIN      
        1 acactcctct taatatacag tcctattgta taaagtgtta ctatatacat agtaactgta
       61 tacataaaaa aagtcaagta ttagaaaaaa gttcaaaaga tcaagtgtgc gcttcgctcc
      121 cactcccctc gacgttttag tagcctttcc ctcacttcgt tcagtccaag ccaactaaaa
      181 agttttcggg atacattctc cttctcttcc taataatcaa ttaaaatttt aatctttata
      241 tttctgctaa tcactcagga aacagtacag aaaaacaaac acgtatcata gatataaatg
      301 taatggcata gtgcgggttt tattttcagc ctgtatcata gctaaacaaa tcgagttgtg
      361 tgtccgtttt tgggcgttct gctagcttgt ttaaagtctc ttgaatgaat gtatgttcta
      421 agtcaaaaaa ctctgacagc gcctttatat agataaagtc cgtataattg tgtaaaagta
      481 aaaaggccat ataacagtcc ttttacggta caatgttttt aacgacaaaa acatacccag
      541 gaggactttt acatgaccca agtacatttt acactgaaaa gcgaagagat tcaaagcatt
      601 attgaatatt ctgtaaagga tgacgtttct aaaaatattt taacaacggt atttaatcaa
      661 ctaatggaaa atcaacgaac agaatatatt caagcaaaag aatatgaacg aacagaaaac
      721 cgacaaagtc aacgaaatgg ctattat
//
LOCUS       DACEPD010000220          673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.231, whole genome shotgun sequence.
ACCESSION   DACEPD010000220 DACEPD010000000
VERSION     DACEPD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.231"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            250..663
                     /locus_tag="IW797_002828"
     CDS             250..663
                     /locus_tag="IW797_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ1225543.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          273 a           78 c           88 g          234 t
ORIGIN      
        1 aggggcggta cataaaaatt tattatttag attctataaa catgtgcaac ttatctaaca
       61 acaaagtata tattctacag aagtaaacaa atagcttata ttttaggatg tacacaaatt
      121 gacaagtata ataaaacttt gataaaatac aaatgttttt tatttttatg tgtgattagt
      181 tagaacttac ttaaatccta ttataatatt tttgttaatg attttaataa aatgattgga
      241 gagaaaatga tggatcaaaa agcgtttatc gaatctattg attctacaat cgatagatta
      301 aaaaaagaaa ttcgatttta taatagagta attggtatcg gaaatattat aaaaatagtc
      361 ttatcagcta gtatcccaat attaattgat caggcttccg aacacaggtc tttactattg
      421 attgtttcaa ttgcttcagc aataattaca attatacaaa gtgggatgtc tgcatttaat
      481 tatcaagata aagtacagac ttcgacagag ttattaatga aaattgaaaa agaaaagttg
      541 ttatatataa cgaaaacatc cccatataat aaaacagatg aagaaaattt ccatttaatc
      601 gttacaaccc ttcaaactga attaaatgat attatctctg attttaatca agttaataac
      661 taaaaattta ttt
//
LOCUS       DACEPD010000221          671 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.232, whole genome shotgun sequence.
ACCESSION   DACEPD010000221 DACEPD010000000
VERSION     DACEPD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.232"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IW797_002829"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IW797_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ1225544.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..>671)
                     /locus_tag="IW797_002830"
     CDS             complement(481..>671)
                     /locus_tag="IW797_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225545.1"
                     /translation="KLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNG
                     INNYILQAILKELLSNAL"
BASE COUNT          202 a          145 c          111 g          213 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta t
//
LOCUS       DACEPD010000222          666 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.233, whole genome shotgun sequence.
ACCESSION   DACEPD010000222 DACEPD010000000
VERSION     DACEPD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.233"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            55..666
                     /locus_tag="IW797_002831"
                     /pseudo
     CDS             55..666
                     /locus_tag="IW797_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          274 a          112 c          125 g          155 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattga
//
LOCUS       DACEPD010000223          638 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.235, whole genome shotgun sequence.
ACCESSION   DACEPD010000223 DACEPD010000000
VERSION     DACEPD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.235"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            33..>638
                     /locus_tag="IW797_002832"
     CDS             33..>638
                     /locus_tag="IW797_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ1225546.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTE"
BASE COUNT          233 a          121 c          108 g          176 t
ORIGIN      
        1 gacaaccaat tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc
       61 aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga
      121 taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca
      181 ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat
      241 atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt
      301 taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa
      361 aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata
      421 ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat
      481 ccagagcctt aaatgataag tatgtcgcta tttttatgga cgctacttac attccactaa
      541 aacgtcaaac cgtatccaaa gaagcgattt atattgccat tggtatacga gaagacggca
      601 ctaaagaagt actgagttat gcgattgctc caactgaa
//
LOCUS       DACEPD010000224          608 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.236, whole genome shotgun sequence.
ACCESSION   DACEPD010000224 DACEPD010000000
VERSION     DACEPD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.236"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..138
                     /locus_tag="IW797_002833"
     CDS             <1..138
                     /locus_tag="IW797_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAQ1225547.1"
                     /translation="LIRNYKISGGGCYLLECRFTSKKSMNSFLEQLNEYANYKLSIVI
                     D"
     gene            complement(327..>608)
                     /locus_tag="IW797_002834"
     CDS             complement(327..>608)
                     /locus_tag="IW797_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ1225548.1"
                     /translation="IHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIE
                     RLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          201 a           89 c          103 g          215 t
ORIGIN      
        1 ttgattagaa attataagat tagtggaggg ggctgttatc ttttggaatg tagatttact
       61 tcaaaaaaat caatgaacag ctttttggaa cagcttaatg aatatgcgaa ttataaatta
      121 tctattgtta tagattaata ataaaactag cagatggtaa aaatactaat atgcgagaca
      181 cacattcaac cataactata taacttatca atttaaaact aaactgatgg aaacaagtaa
      241 ggcaaaaaca tttatatttt gccctactta ttttttagtc aagtccagac tcctgtgtaa
      301 aatgctatac aatgttttta ccatttctac ttatcaaaat tgatgtattt tcttgaagaa
      361 taaatccatt catcatgtag gtccataaga acggctccaa ttaagcgatt ggctgatgtt
      421 tgattgggga agatgcgaat aatcttttct cttctgcgta cttcttgatt cagtcgttca
      481 attagattgg tactctttag tcgattgtgg gaatttcctt gtacggtata ttgaaaggcg
      541 tcttcgaatc catcatccaa tgatgcgcaa gcttttgaat attttggttg atcgatataa
      601 tcatgaat
//
LOCUS       DACEPD010000225          602 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.237, whole genome shotgun sequence.
ACCESSION   DACEPD010000225 DACEPD010000000
VERSION     DACEPD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.237"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(318..581)
                     /locus_tag="IW797_002835"
     CDS             complement(318..581)
                     /locus_tag="IW797_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAQ1225549.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          226 a          104 c           79 g          193 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tctatttttt
      601 ta
//
LOCUS       DACEPD010000226          595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.238, whole genome shotgun sequence.
ACCESSION   DACEPD010000226 DACEPD010000000
VERSION     DACEPD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.238"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..>595)
                     /locus_tag="IW797_002836"
     CDS             complement(<1..>595)
                     /locus_tag="IW797_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1225550.1"
                     /translation="NKKKVQRLIKKLGIKVTAYTRKSRRYNSYRGKVGAVAKNRIHRR
                     FYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMDMFNSEILSYRISEKPNAL
                     AVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLKEYKIFQSMSRKGNCLDNS
                     LMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNN"
BASE COUNT          164 a          117 c          105 g          209 t
ORIGIN      
        1 cattattgta gtaatagata tagctatcga tggctgattt taattcctcg aaacagttat
       61 aaactttccc gtgaaaaatc tcttgtttta ataagccaaa aaaattctcc ataagtgaat
      121 tatctaagca gttccctttc cgagacatgc tttgaaagat tttgtactcc ttcaatttgt
      181 ttctataagc tttcatttga tacgcccaac cttgatctga gtgaaacgta cgacgatagg
      241 gacaatcgtt agtggtctgg atagcttcct ccaaaccttc cattaccgct aatgcattgg
      301 gcttttcaga gatacgatag gataaaatct cactattgaa catatccata aaaggatcta
      361 gatacaattt cttttgacgg ataatgcctt tggcatccac ttcatggtat ttaaattcag
      421 ttgtatccgt cgtgattttc tgatggcaga tactggtgta gaaacgacga tggatccgat
      481 ttttggctac tgctccaact tttcctcgat aagaattgta gcgacgtgat ttacgcgtgt
      541 atgccgttac ttttattcct agcttcttga tcaagcgctg aactttcttt ttatt
//
LOCUS       DACEPD010000227          593 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.239, whole genome shotgun sequence.
ACCESSION   DACEPD010000227 DACEPD010000000
VERSION     DACEPD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.239"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..580
                     /locus_tag="IW797_002837"
     CDS             <1..580
                     /locus_tag="IW797_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ1225551.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DACEPD010000228          580 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.240, whole genome shotgun sequence.
ACCESSION   DACEPD010000228 DACEPD010000000
VERSION     DACEPD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.240"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
BASE COUNT          182 a           96 c          133 g          169 t
ORIGIN      
        1 tgaaacacgt tagaggagcg aaaatggagc ctgtaaggcg actggtagcg gaacggtcgt
       61 gttagattgt gtagccgata gctgaacgaa ataaatgctg gcagcgccat tctatttcgg
      121 caggaggagg ctcaaggggt tcggggaatg aaattccctg aggtcttttt attttttcta
      181 gaggagtgct ataatcaaag aaaatgagaa aaaagagtaa aaaaatagtc gagcagtgtt
      241 gacgcactct cgacctttac ggaaactact tcacctagtt ccgtaactca agttcattat
      301 acgaatattt ttaggtttct tcaagctaga gatttttgga tgtggatttt tagagttacg
      361 tgaactctgg aaattctttc caagaatctc ttttttcgtt gaggaggagc tatttttgag
      421 taaaaaaggc ataatcgtaa atgagtggga aaaacttaac cgtatttcta acaggcggtt
      481 aaatgcacaa actatgtgca cccccttaaa aatgcaatgg tcaattgacc gaattaccat
      541 tgttggtaca ttaagggaaa atatttatta tcacactccc
//
LOCUS       DACEPD010000229          567 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.241, whole genome shotgun sequence.
ACCESSION   DACEPD010000229 DACEPD010000000
VERSION     DACEPD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.241"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>567
                     /locus_tag="IW797_002838"
     CDS             <1..>567
                     /locus_tag="IW797_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ1225552.1"
                     /translation="EDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFTKEMTH
                     DEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGEREK
                     QFAYSAHTSCDDNGFILSTIITPGNIHDSQVAFQLVKQSKRLFPEINCVVADAGYKTP
                     KFVHFLTHLNLRPCLPYSRPKGKKGLLS"
BASE COUNT          194 a          106 c          114 g          153 t
ORIGIN      
        1 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
       61 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
      121 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
      181 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
      241 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
      301 agaaaaacaa tttgcttatt ctgcccatac ttcatgcgac gacaatggat ttattcttag
      361 cacaatcatc actcctggaa atattcatga cagtcaagtc gcctttcagc tcgtcaaaca
      421 atcgaagaga ctttttcctg aaatcaattg tgttgtcgct gatgctgggt ataagacacc
      481 taagtttgtt cactttttga ctcatttgaa ccttcgacct tgtttgcctt attcaaggcc
      541 caaagggaaa aaggggctat tatcaaa
//
LOCUS       DACEPD010000230          563 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.242, whole genome shotgun sequence.
ACCESSION   DACEPD010000230 DACEPD010000000
VERSION     DACEPD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.242"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>563
                     /locus_tag="IW797_002839"
     CDS             <1..>563
                     /locus_tag="IW797_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1225553.1"
                     /translation="LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYP
                     KTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRA
                     YQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSI
                     EIIGSGYDSVAYLVNNEYIFKTKFSTN"
BASE COUNT          230 a           50 c          100 g          183 t
ORIGIN      
        1 cattaaaaaa acattataca gagccttggg aagatgaagt ttttagagta attattgaat
       61 ataacaatgt tcctattgga tatggacaaa tatataaaat gtatgatgag ttatatactg
      121 attatcatta tccaaaaact gatgagatag tctatggtat ggatcaattt ataggagagc
      181 caaattattg gagtaaagga attggtacaa gatatattaa attgattttt gaatttttga
      241 aaaaagaaag aaatgctaat gcagttattt tagaccctca taaaaataat ccaagagcaa
      301 taagggcata ccaaaaatct ggttttagaa ttattgaaga tttgccagaa catgaattac
      361 acgagggcaa aaaagaagat tgttatttaa tggaatatag atatgatgat aatgccacaa
      421 atgttaaggc aatgaaatat ttaattgagc attactttga taatttcaaa gtagatagta
      481 ttgaaataat cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt
      541 ttaaaacaaa atttagtact aat
//
LOCUS       DACEPD010000231          479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.denovo.245, whole genome shotgun sequence.
ACCESSION   DACEPD010000231 DACEPD010000000
VERSION     DACEPD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.denovo.245"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..430)
                     /locus_tag="IW797_002840"
     CDS             complement(<1..430)
                     /locus_tag="IW797_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ1225554.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          135 a           95 c           64 g          185 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tctaactttt ggggtgcac
//
LOCUS       DACEPD010000232        23734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.guided.040, whole genome shotgun sequence.
ACCESSION   DACEPD010000232 DACEPD010000000
VERSION     DACEPD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.guided.040"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            292..567
                     /locus_tag="IW797_002841"
     CDS             292..567
                     /locus_tag="IW797_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225555.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            580..2658
                     /locus_tag="IW797_002842"
     CDS             580..2658
                     /locus_tag="IW797_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ1225556.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2818..4704
                     /locus_tag="IW797_002843"
     CDS             2818..4704
                     /locus_tag="IW797_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ1225557.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4716..5663
                     /locus_tag="IW797_002844"
     CDS             4716..5663
                     /locus_tag="IW797_002844"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ1225558.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5683..6210
                     /locus_tag="IW797_002845"
     CDS             5683..6210
                     /locus_tag="IW797_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ1225559.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6273..6926)
                     /locus_tag="IW797_002846"
     CDS             complement(6273..6926)
                     /locus_tag="IW797_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ1225560.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7058..7900
                     /locus_tag="IW797_002847"
     CDS             7058..7900
                     /locus_tag="IW797_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ1225561.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8058..8954
                     /locus_tag="IW797_002848"
     CDS             8058..8954
                     /locus_tag="IW797_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ1225562.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8957..9670
                     /locus_tag="IW797_002849"
     CDS             8957..9670
                     /locus_tag="IW797_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ1225563.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9690..10208
                     /gene="msrA"
                     /locus_tag="IW797_002850"
     CDS             9690..10208
                     /gene="msrA"
                     /locus_tag="IW797_002850"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ1225564.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10205..10432
                     /locus_tag="IW797_002851"
     CDS             10205..10432
                     /locus_tag="IW797_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ1225565.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10584..11135
                     /gene="lepB"
                     /locus_tag="IW797_002852"
     CDS             10584..11135
                     /gene="lepB"
                     /locus_tag="IW797_002852"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ1225566.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11218..11760)
                     /locus_tag="IW797_002853"
     CDS             complement(11218..11760)
                     /locus_tag="IW797_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ1225567.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11997..12395)
                     /locus_tag="IW797_002854"
     CDS             complement(11997..12395)
                     /locus_tag="IW797_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ1225568.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12655..13515
                     /gene="ylqF"
                     /locus_tag="IW797_002855"
     CDS             12655..13515
                     /gene="ylqF"
                     /locus_tag="IW797_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ1225569.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13508..14275
                     /locus_tag="IW797_002856"
     CDS             13508..14275
                     /locus_tag="IW797_002856"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ1225570.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14331..15188
                     /gene="dprA"
                     /locus_tag="IW797_002857"
     CDS             14331..15188
                     /gene="dprA"
                     /locus_tag="IW797_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ1225571.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15310..17388
                     /gene="topA"
                     /locus_tag="IW797_002858"
     CDS             15310..17388
                     /gene="topA"
                     /locus_tag="IW797_002858"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ1225572.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17357..18733
                     /gene="trmFO"
                     /locus_tag="IW797_002859"
     CDS             17357..18733
                     /gene="trmFO"
                     /locus_tag="IW797_002859"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ1225573.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18931..19836
                     /gene="xerC"
                     /locus_tag="IW797_002860"
     CDS             18931..19836
                     /gene="xerC"
                     /locus_tag="IW797_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ1225574.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19873..20421
                     /gene="hslV"
                     /locus_tag="IW797_002861"
     CDS             19873..20421
                     /gene="hslV"
                     /locus_tag="IW797_002861"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ1225575.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20435..21835
                     /gene="hslU"
                     /locus_tag="IW797_002862"
     CDS             20435..21835
                     /gene="hslU"
                     /locus_tag="IW797_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ1225576.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21853..22644
                     /gene="codY"
                     /locus_tag="IW797_002863"
     CDS             21853..22644
                     /gene="codY"
                     /locus_tag="IW797_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ1225577.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22726..23601
                     /locus_tag="IW797_002864"
     CDS             22726..23601
                     /locus_tag="IW797_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ1225578.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8214 a         4092 c         4999 g         6429 t
ORIGIN      
        1 ttctgattca tcagtcccat aaattataga gccgattttt gtcgcagccc catttttttg
       61 ttgtgataaa agaacaagaa aggcaggaat gggaaagtaa acatcctttt gtcagaaact
      121 tttatctttc ttaagtttta cttgatatct gatactattt ggaatagaat gaggcgttga
      181 aaaatttatg gaaatttcta actagcaata aacgatgaaa caatgaaata tttttcatcc
      241 aaaaagaact ttgctataat gatggaagtt tgtgtatagg agatctggaa aatgagagag
      301 agttttgaac aacaaaaaaa actgttgcat gatcgctatg gcgccctttc gatggatgac
      361 cgccgtcaaa tactttgtaa gttgcgtaaa agaaatatat tgatgtatcg tcagttggaa
      421 cgtctgaaac atgatctgct tcgtttggag tcaaaacgtg tacagtgtga attagagggg
      481 aaccaaacac aggttgaagt agtcgaaacc aaaattttaa agaaaaaaga acaattttta
      541 aaaatgctta cacaaaataa aaaataaagg ggatggacga tggaatttgc tgagttagtt
      601 attgtatttg cagtaacgat caccttatcg aacatagcta gccgcatttt gccaatgatt
      661 ccagctccac tgatccaaat tttccttgga gtggtattag gtctgacaga gtggggacaa
      721 tcaatcgatt ttgaacctga attgttctta gttatgatta ttgcaccatt gttatttaga
      781 gaaggagaaa aagcagatat ttcttcgatt ctcaaaaatt tcgggacaat tttgttcttg
      841 gcatttggag gagtcatttt gacccttgtc ggggtaggtg cgacgttatc attcctgttg
      901 ccaagtgtcc cacttgctgc ctgctttgct tttggagcag cgttaggtcc gacagatgcg
      961 gtagcagtca gttctctttc tggacgagtg aatatcccta aaaaggcgat gcatattttg
     1021 gaaggagaag gtcttttgaa tgatgcatct ggtgttactg ccttccagtt tgctcttggt
     1081 gcgctgatca ccggatcttt ttcagcagta aacgcgggga tgagtctagt agtttctagt
     1141 attgggggag ctttgatcgg ctttttgctt gtctggttca aacaaaaaat aattcatttg
     1201 atcgaaaaag catcagcaca agatgtcaca gggtatctgc tgatcgaact tttgcttcct
     1261 tttctagcct atgtattggc agaggcattt gatgtatctg gaatcattgc tgcagtggca
     1321 gcaggtattc tccaagcttc tggttttagg aaaatctctg tgtttgatgc agaattgtcg
     1381 agcttgtccc acagtacatg gacaactatt gcttttacac tgaatgcatt agtatttata
     1441 tttttaggaa tcgaattgac gcaagtgttc tcgccagtat ggggagacgg tttatatcca
     1501 aacggtcttt tattagcgat tatcgtatta atctctgtca tgctttttgt gatcagattc
     1561 atttctatca gtctttttta tgtattcaaa gatggcagta aaaagttcaa aaaacagttg
     1621 aacgagatct tgatcttaac atttggcggt gtaaagggaa cagtcagttt ggctacgatt
     1681 tttattttgc cattttcaat caataatatg atgttttacc agcggtcttt gcttttgttt
     1741 ctgacggcgg gtgtcatctt agtgactttg gtcataggga tcattgtctt gcctatgctg
     1801 acggaaacag aggaagcgaa aagcacggat ttgaatgctt tgatgatttt agaagaggtc
     1861 gttgaagttt tgcgaaaaga aataaaggaa attgatcgta atacaaaaga atttttagcc
     1921 actgaagcag tgatagaaaa ttatcaagaa cggatacgtg atctgtactt ggaagattta
     1981 accgatgatg aaaagcaaga agtacaagaa atacaagcac tgattttgtc gatcgaacgg
     2041 gatggattgg atgaaagtta tcgttcgggc aagctttctt ctaatggtta tcgtttttac
     2101 tcccgttttc tttctcgatt cgaacattct atcacaagcc agattttgtc ttttatcggc
     2161 ttttggttca ttgttgtccg taggttaatg cgaatcgttc tccatccgaa aatgttttgg
     2221 caaagaagat atgctgatcg tcagacattt attagtgatg aagatatcca agaaatcaga
     2281 gagacatatc aaaaaaatac tcagttgatt attgaaagtt tagataactt gactgatatt
     2341 tatgatgaaa cgatggttca attttttatc cagcaacgca aacttgaagg aataaaaatg
     2401 aactcgggga atttgatttc ttcgtggatg atccagcaag atagtctgtt tacgaaaaaa
     2461 atgctaagag gttattatct tgaaagaaaa atcattgatg aatacgaagt tgctgaaaaa
     2521 atcacgacgt tttctgcgaa cagctatcgc aggaatatca atttgttaga atcttatacg
     2581 atgaacaaac catctgataa cttttctttc cgatttgcgt ttcgagcaaa gtcaaaaaaa
     2641 acgagtccaa taaaataaaa aggtaaaaat tttcaagttt tataaaaaac aaaacatatc
     2701 agaacaggat cgaatcggtc ctgttctttt tttgagaaca caaaaagaac aaataccaaa
     2761 aaaattttcc tgaagttcac tatttttatg atacaatatc caagataagg agggaacatg
     2821 ttgaaagaat taaaagtaac tgacctcaaa aaaacttatg gtgaaaaaga tttgtttgac
     2881 cagatttctt ttttggtcca cgaaaaagac cgtatcgggt tgattgggac gaacgggacg
     2941 ggtaaaacaa gtctcttaaa tattattgcc ggtatcgata gcagcgacgg tgatcggcaa
     3001 acagtgtttt atccaactga ttaccgtatt ggttatcttt ctcaagcagc tgaattttcc
     3061 gatgaattaa ctgttctcca agcagtcttt caaggaaaca gccccttgat ccaaactgtt
     3121 cgagcatatg aggaagcatt gatcgaacta ggggaaaacg gagaagaccc tgatgtgcaa
     3181 aaacgttatg ctaaagcaga agaacaaatg aataaagaag atgcttggac tacagatacc
     3241 aacgcaaaga ttattttgca aaaattagga atcagtgaat tagataaaaa aatcagtact
     3301 ttatcaggtg gtcagaaaaa acgagttagt ctggcgcaag tgttgattga tgaacccgat
     3361 ttactattac tagacgaacc aacaaaccat ttagattatg aagcgatcga gtggttggaa
     3421 aactatttga aacaataccg tggcgcacta ttgatggtta cccacgatcg ttattttcta
     3481 gatagagtag caaaccgaat cttcgaactt tcatttggaa aattatacga gtataaagga
     3541 aattatgaag cttacgtctt agaaaaagca gaacgtgatc gtgtagaagt agaacaagaa
     3601 gaaaagcgca agcggcttta taaacaggaa ttagcttgga tgagggctgg cgtacaggca
     3661 cgtggcacca agcagcaagc acggatcaac cgtttcgaag atctaaagga gaacttgtac
     3721 caagtaaacc aagaagatga tttagagctg aacctagcga cacaacgttt agggaaaaaa
     3781 gtactggaaa tcaaagatgg cagttaccgt ataaatgacc aaacactgct agaacattta
     3841 gatttattga ttcaatctcg ggaaagacta ggtattactg gtaaaaatgg tgcgggaaaa
     3901 tcgacattgc ttaatattct agctggcaga attcccctgg atagcggtac aatgtctatc
     3961 ggtgaaacgg ttcatcttgc gtattataca caagaaaatg aagaaatggc accagataaa
     4021 cggatgattg cttacttaca agaagctgct gaagaagcaa aaacagcaga tggcagtcaa
     4081 atcggagtag cagaattact tgaacgattt ttatttccaa gatttatgca tgggacactg
     4141 ataggcaaac tttcaggcgg agaaaaacgc cggttatttt tgttgaaact gctgattcag
     4201 caacctaatg ttttattatt ggatgaaccg accaatgatt tggacatagc aacattgacg
     4261 attttggaag attattttag aagttttcca ggtgctgtga tcactgtttc ccatgaccgt
     4321 tatttcttgg acaaggtagc agataagtta ttagtattcc aaggaaacgg aaaacaagaa
     4381 ctttattacg gaaacatgtc cagttattta ctgaagcaaa aagagaccca gcagcctgct
     4441 gaaaaagcga aaccaaaaat acagagcaag gaacctgctg aaaagaagaa attatcttac
     4501 atggaacaaa aagaatggga aacgattgaa gatgagattg cagaacttga agaaaaaatc
     4561 tcattgctcc aagaagagat gaatcatcaa ggagataatt tcactcgatt acaagaattg
     4621 caaaacgacg tatctgaaac agaagcacaa cttgaagaaa aaatggcacg gtgggaatac
     4681 ttgagtgaat gggtagaaga ttaggaggaa aaaggatgga acaagcgtat cttgacttag
     4741 gaagaaaatt gttggaagaa gggcatcgta aagaagacag aacaggcaca ggcacattaa
     4801 gcttattcgg ataccaaatg cggtttgatc tgcaaaaagg gtttcctttg ttaacaacga
     4861 aacgagtacc ttttggattg atcaaaagcg aattgctttg gtttttaaaa ggagatacga
     4921 atatccgcta tctattacaa aataaaaacc atatttggga tgaatgggca tttgaacgct
     4981 acatcaaaag tccagactat aatggaccag atatgactga ttttggccat agatgtttga
     5041 cagatcctga attcaatgaa gaatataaaa agcagagcaa gcttttttgc gagaagatcc
     5101 taaccgatga ttcctttgct gaaacatacg gcgatcttgg acacatctac ggctatcaat
     5161 ggcgtcactg ggaaacaaaa gacggtggct ttatcgatca aatcaaacaa gtgatcgaag
     5221 caatcaaaaa gactcctgat tctagaagat tgatcgtttc tgcatggaat ccggaagatg
     5281 ttcctagcat ggctttgcct ccttgtcata cgatgtttca attttatgtt caagagggac
     5341 ggctaagctg ccaattgtat caacgcagtg gggatgtgtt tctaggcgtt ccgttcaaca
     5401 ttgcaagtta tgcgttgctg actcacctaa ttgcacatga aacaggttta gcggttggcg
     5461 aatttgttca cacattagga gatgctcatc tttatatcaa tcatatcgaa caaatgaaag
     5521 aacaattgag ccgagaaata cgcgcatttc cgactttagt tttgaatcag gaaaagcagt
     5581 cagtatttga ttttgagatg gaagatatcc aattagaagg atatgaccca catccggcga
     5641 tcaaagctcc tatcgctgtt tagaatcaga aaggatgaga aaatgttagc tgcaatttgg
     5701 gcacaggatg aaaacggatt gattggaaaa gaagaccgtc ttccttggcg tttgccaaat
     5761 gatctgaaat tcttcaaaca aatgacagaa gcaaatacac tagtgatggg acgaaaaaca
     5821 ttcgaaggga tgggtagccg tccgttgccg aatcgtcaga cgattgtttt gacgagagat
     5881 tcttcctata aagcagaagg cgtgcatgtc atgcatagtg tggaagacgt gcttgctctg
     5941 gaaaaagaaa cagacggtat ctttttcatc gctggagggt cagctgttta tcaagaattt
     6001 ctgccatttt gtacgatatt ataccgtaca gtcatccatc atgtattcga tggagatgct
     6061 tattttccac ctgttgactg gtctgattgg tcactgatca atactagtca aggagaaatg
     6121 gatgaaaaaa atcgttatcc gcatcaattt gaaacgtatc agcgtaatga gaatcctgta
     6181 aaaagcggga caggcgaatt gacgatataa aaaagctatg gatagactag ctgttattta
     6241 gagctgtccc atccatagct ttattttttt tattaagtaa agaataaaac agagaagtac
     6301 ataaaacctg cacctaacat aacaaacaaa tgccaaacaa catgcatata gcggatagaa
     6361 ttcaagctat aaaagaatgc gcctatcgtg taagaaagcc cgcctgctac catcaaaata
     6421 acaccagtca ttccaagaga gtgataaaga ggaacagcag ccacgacaca gagccacccc
     6481 ataaaaatat agatgatcgt agatactttt gatactctct cccgtttatg caacgtcaac
     6541 gctttatata taatgccaag aatcgcaaac aaccagatta atacaaataa agtccaacct
     6601 agccatcctc ggatactcag caaacaaaaa ggagtataac ttcctgcaat caaaagaaaa
     6661 atcgaactat gatcgaatac ttggaacacc ttttttgctt ttgtaaaaat caatgaatga
     6721 aacaaagtag acgttaaaaa taaaagaatc atcatcgatc catagatagc ataagagaca
     6781 atatggaggg aggaatgcaa gtgggcacct tttactaaaa gaaccactag tcccgcgata
     6841 ctcaatccag tcccaattcc atgggtgact gcattaaaca cttcatttaa tatttcatac
     6901 gttcgattaa atttcgtttt ttccatgaaa acggctcctt tactaggtgt aattaatgaa
     6961 aaatcattat ttgtctgtta tactaaaaat agaaattgtc ttacatgtag tttaacatga
     7021 ataaggcatc ttgttaaaga aagagagaga gaaaataatg acaaatgtaa aaatcgtaac
     7081 agattcttca tgtacgatgg aaaaaaatgt acgcgatgct ttaggcattc gtgtcatgcc
     7141 gctatccatc atgatcgacg gaacagtata tcccgatgat gaccaactag acggcgaaag
     7201 atttatggaa atgatggcga aagcaaaaaa tctgcccaaa acaagccaac caccaattgg
     7261 agaattcatc tcgatgtatg atgagctagg agaagacggc agtgaagtga tttcgatcca
     7321 tatgacaaaa ggcttgagtg gaacagtaga agcagcaaga caagccagta atctaacaaa
     7381 aacgaaagta acggttatcg acagtgatac cactgaccaa ggattatctt ttcaggtaat
     7441 tcgagcagct gaattagcaa aagaaggtaa aacagtagag aaaatccttg aagaagtcaa
     7501 aaaagtacaa gacaatacaa aattatacat cggaatatcc acactagata acttggtaaa
     7561 aggtggaaga atcagccgaa caactggttt gctgtctagt gttttgaaca tacgcgtggt
     7621 gatgaatttc gcccattctg aattaatccc tgtaactaag gggcgaggaa agaaaacgtt
     7681 cgataaatgg tttaataatt tgaaaaatga actgaaagaa ttgccaaatg tgcgacaaat
     7741 cggtatttca catgcggatg caaaagaatt ggcgcaagaa ttcaaagagg gtttgcaaga
     7801 attattcccg gacatgcata tccctgtact gcatacgaat ccggttatcg ctacgcatac
     7861 tggtaaagac gcgtttgcaa tcatgtatta tgtagattag tcgaaaagag gataagccct
     7921 cagcctgaaa ggcttgcagc ttattctctt tttctttata aaattaggaa aataattgtt
     7981 ttcttttttc ataaagacta cctttttgat aaaatttttg ataacattag aaccaatcga
     8041 atacaagagg tgaaaaaatg aaattaacga aacgacattt gatcctcgtt ttgttcccat
     8101 tattaattgc gatcatcatc tatgcaggtt tagctgcttc tataccgaaa gctggcccac
     8161 taatgaaacc agtaactact gtgagagaag atccaaataa aaaagaaaaa cttcactaca
     8221 tagctatcgg agattcgctg actgaaggag taggagatca aaccaagcaa ggaggattcg
     8281 ttccccttgt cgcaaatgat ctaaaagacc gttataattt gaccgcggtt gaaacagaaa
     8341 actatggtgt aaatggtgaa cgaagtgacc agatcttaaa gcgagtaaaa acaaaagaag
     8401 aaattcgaaa caatatcaaa acagctgatt ttatcacact gactgttggc ggtaatgatt
     8461 tgatgaaagt tatccaaaat aatttattcg ggttaacggt caattccttt aaaaagccac
     8521 tgaaaaacta tcaggaaaat gtcacagaac tattggaaga atttcgatcc ctcaataaaa
     8581 ccgcacctat ttacatttta ggcatctaca atccatttta tttgaacttt cctgagatca
     8641 ctgacatgca gacaattgtt aacaattgga acaaaggaac agaaacggtg acaaaaaaaa
     8701 tgccgaactg ctactttatc ccaatcaatg atttgcttta tcaaggattg aatcaggaag
     8761 taggtgtcac agaagaagga acagatcaaa caactagcac gacagaaaat ctaaatggca
     8821 tcaaaaataa tgtgctctat gaagaagatc atttccatcc aaataattta ggttatcaat
     8881 tgatggcaaa tgcagtgaga gatcagctga tcgagacaca aaaccaatgg ttagagaagg
     8941 aggatgatca ataaacatga aaacaagatc tgacgtatct tcaaacaaaa caaagaaaac
     9001 aaattactgg aaattcgcct tccttatcct tcttggggta gtacttggtt cgaccgtatt
     9061 tttaggcagc cgtatttttg ctaatcggga accagaactc ccagaaatac cagctcttac
     9121 ggaaagacaa ggagatcctg tcttaacgat caattcaaat aaagaaaaag tcaatcaaat
     9181 catcagcttt ttccttagtg agtatcaaaa agatagtgat attgaatata aattttattt
     9241 ggaaaatgaa gcactactca atggtacttt cgaagtacta ggttttccta tcgatttcta
     9301 tttatatttt gatccgtatg tgatggaaaa cggaaatgtc caactaaaag ctagaagtct
     9361 ttctatcgga acattgagtt tgccaatcaa agatgtgatg aatatgatca agagaaacta
     9421 taaattacca gattggatcg aagtcaatac agatgatctg actatcatgc ttaggttgga
     9481 tcaattccga atgcaaaacg ggatgtatat taaagcagat aaaatcgact tagtaaatga
     9541 tgacattcgg tttagtctat atttgccaaa agatacctct gatgcttcaa acaactcaaa
     9601 taccaacaaa actagcacca gcagtactga aagcaagaca aaagaaaata aaaaatcatc
     9661 ggatgagtag tattagaggg ggaatcataa tggaacgagc aatatttgca ggaggctgct
     9721 tctggtgtat gatccagcct ttcgacacgc ttcctggtat ccatacagtg atgtcaggat
     9781 atactggagg gcatgtacca gatccgactt atgaacaagt aaaaagccaa acgactggac
     9841 atacagaagc agtggagatt atctatgatc caacattgat ttcatatgaa caattagtag
     9901 aactttactg gcagcaaact gatccaactg acgctttcgg acaattcgag gatcgcggtg
     9961 acaattatcg cccggtgatc ttttatttga cagaagaaca gcggatcatt gctgaaaaaa
    10021 gtaagaaaca gttagctgaa agtggacgtt tcgaacagcc aatcgttaca aaaatcgaac
    10081 cagcccaacc attttatcta gcagaagaag accatcaaga gtattataaa aagcatcctg
    10141 aaaattttaa aagaaaccat gcacgccggg ctgcctttat cgaagaaaat tggaggaacg
    10201 aagggtgaat cgaagctttt ttcattattt aatgacactg cgaggcggtg gcacacatga
    10261 tgctttatct gttttcgcta cagaagccgg taaagacatc caatttccta agcacagctc
    10321 tagctacgaa gaaatttcta attatctgga actgaatgtc gactatttgc cgtccatgga
    10381 tatctttgac gaagcatggg aaaaatattt agaaaataat catgacatat aagagatagt
    10441 tttgtacttt atgatcaaaa aagttttttg tccaatagcc taattgaaag aattattttt
    10501 atttcagcta tctagtcgca tttttttgat atttttctta taataaagaa agaatgaaaa
    10561 aaagagggat ggattgatag agattgacaa aaaaacaacg ctatattaat cgcttctggc
    10621 ttgtattcaa atacttactt gtttctgtat tccttgcgtt catgttgcgg ggatttttat
    10681 tcatcccggt accagtggaa ggcaattcaa tggaaaacgt gttgaagcaa ggtgatatgg
    10741 ttgtgatgga aaaattttcg gagatcagac gatttgatat tgttgttttt caattagcag
    10801 atggcacgat ttatatcaaa cgagtaatcg gtcttccagg agaaaatgtc agctaccaaa
    10861 atgatcaatt gaagatcaat gggaaagtag ttaaggaacc ttatctaact aaaaacctga
    10921 aatcagatca tgcaaatgca tcctatacga cggactttac actacaagag ctaacgggac
    10981 aatccaagct tccggaagat aattattttg tacttggaga taatcgaaga gtttcaaaag
    11041 atagtcgttc gtttggcacg atcaataaaa ctgatattct aggtaaagca agatttgtct
    11101 attatccact tgatgaaata aaatggatcc agtgagaaac aataaagcgt gcagtctgtt
    11161 gagactgcac gctttattgt ttaatccaaa atcagtttac taccttagta cgtaaaatca
    11221 aacagctcgt ttaaacactt ttctagcctc taaagttttg ataaatgaat aaacaaccat
    11281 tatttgaata gggatttgga tcatattcct gatgaatcta cctagaaaaa ttactttgaa
    11341 atctgcacca aacaaaacag acatcagcca agaattgata aaaaggctga ctgggatcat
    11401 aatcaataat tgggcagcga tgacggaagg caggtttatc tttttatgat ataaaaataa
    11461 gccattaata aatccaccaa ggaaagccgt gataagaaaa gcagcttgaa acgttcctga
    11521 accaaatatc gtgaaattta aagtgttcga caacatttca agcaaaccag cccagatcgg
    11581 tccatataga taacccataa gcgccatact taaaaatccg aatgtgattt gaaaataagc
    11641 aggaccaatc gctatcttgt ataagatgac atcaagggct aataaaagag cagaggtcga
    11701 caacaatctt gcattcattt cgggcaatct aaaaacgtct tttgcaatca aagtgttcaa
    11761 aatattctct cctttgtcag ttaataataa aaggatacca taaaatcacc aatgtgtcat
    11821 gacacattgg tgatttacat acactggcat ctaacaatta aaaaaagggt gagaagactg
    11881 cttttataaa ctgtaaaaaa gagattgtac gaataaaaaa aatagaggcg gagaatactt
    11941 atcgtatcat ccgcctttgt ttttttgtta taaaaaacgt cagattagtt atctgcttac
    12001 atcttcatcc atacgggttc taatgtattt ccgtctagat cttggacttc taatgaaaac
    12061 atttcttctt caggcattcc catatctact tcgtggaaag taccaccatt ttcttttgca
    12121 atttgagcaa atttttttac ttcatccgca ttttcaacac taaacgaaat caaagcacca
    12181 cttgtttttt tattatcagc tatttcttta tcttttataa attttttata aaaatcatga
    12241 gagagaagca ttatccaaaa agtgtcatcc cataccattg aaccagcttc ttcagtagaa
    12301 aattcttcat tttttttaaa acccagcttt tcataaaaag caacggaacg attgacatca
    12361 gcaacaggaa aattaacaaa aaccatacta ttcatttgtt tcgtctcctt tctgttttgt
    12421 tcgtctatca tctcttattt aggaaacgct gtcaaaaatt tagattcata tatagaaacc
    12481 aaaataaaaa tttgcatcat caaaaaagtg aagatgtgat agaatgagga ctggttcact
    12541 ttcacttaac taatagatgg aatttcttta taataaaaaa gtgaatttat attgaaaaag
    12601 aaaaaaacaa gtaaagccgg ggaatattcc ggtatgaaag gaatggagag aacaatgacc
    12661 atccaatggt ttccgggaca tatggcaaaa gcccgacgag aagtttctga aaaaatgaaa
    12721 tatgtggaca tcgtatttga attggtagac gcacgattac ctttatcttc tcgtaatcca
    12781 atgctagatc agatcattca gcaaaaacct cgccttgttt tattgaataa agcggatctg
    12841 gcagatccac aacagacaaa gctgtggcaa gaatattttc gtaaacaagg ccatcttgca
    12901 ttagctatca cggcacaaga aagtaaagga attaaaggtc tagtacctgc ggctaaagaa
    12961 gcattgaaag aaaaacgggc aagagatgct gctcggggga tcaagcctcg cgctattcgg
    13021 gcaatggtct taggtattcc aaatgttggg aaatctacgt tgatgaatcg actagtaggc
    13081 aagaaaattg cacagacagg aaataagcct ggcgtaacaa aaggtcagca gtggcttcgt
    13141 ttaggtaatg agttggaatt gttagacaca ccagggattt tatggcccaa atttgaagat
    13201 gaagaaatcg gcaaaaaact agcgctgaca ggcgcgatca aagaccagct tgtccattta
    13261 gatgatttag cgatttacgg attagacttt tttgcccgat attattcagg aagaatcagc
    13321 gatcgttatg gtttatcaat cgagcaagaa gaaaaattgg caccggaatt actgatggat
    13381 attacagcaa aacgtgggta taaagaagac tatgatcgtg gcagcgaatt agtcgtgcat
    13441 gatattcgtc aaggaaaact tggacgatac acactggatc gttgcgaaga aatggagcaa
    13501 aaaagtgatg actgaatcca tccaaagtat caaagaacga ttgaaaagca tacagagaat
    13561 agatgatcct acactagaat cattaagaaa tgatccacgc aaaggcgtgc agcaagcact
    13621 ggcttctttt gaacgacgat tgaaaaagga agcattaatt aaggaaaaat atgaagaaat
    13681 gttttgcttt gaaaagcaag ggtacgcaca aggtcaccaa tggattgccg gcattgatga
    13741 agtaggaaga ggaccattag ctggacctgt cgtggcagct gcagttatct tgccgaaaaa
    13801 tgagatcata ttagggctga atgactccaa acaattatca gagaaaaagc gagaacttct
    13861 ttatcaacaa gttcaagaaa aagccctggc aatcgggatc gggatcgttg atcagcagac
    13921 aatcgatgat gtgaatattt atgaagcaag taaattagcg atgatccaag cagtagaaaa
    13981 attgtccatt gtaccaagct atttgttgat cgatgcgatg accttggatt tgcctattca
    14041 gcaggaaaaa ataatcaaag gggatgcacg atctgtgtcg attgctgctg caagtatcat
    14101 tgctaaagta tatcgagacc aattgatgaa agaatatgac gtgctttatc ctggatatgg
    14161 tttctcgcat aatgcaggtt atggaacaaa ggaacactta gaaggacttg aacgacaagg
    14221 aattacgcca atccatcgta aaagttttgc acctgtaaaa aaatatttac aataaaaaac
    14281 agccgctttt acgtatctta ttagaaagag agacgaaagg cggttttatg atgtatcaaa
    14341 tagaagaaaa tttattgaaa cttgcttata caaaaggaat cagtagtcaa ggaaaatggg
    14401 cacttgctaa ctggatgatg cagtatcctt ataaagaaat cggatttgaa gacatcatta
    14461 agataggtaa aataaccaaa caccgggagc tgtttcgcaa aagttggcag gaaattcacg
    14521 ctaactgggg caaaatccaa tcgaaacagt cgtttatcac ttgctttgat cccaaatatc
    14581 cccctcagct gcttcacttg acctatccgc caattgtact gttttataat ggtgatctat
    14641 ctttactttc ttgtaacatg ttaagcgttg ttggtggaag acacacttct gctttagcca
    14701 aaaaaacagt ttattattta ctaaaaccag tgatagaagc tggatatgtg atcgtcagtg
    14761 gctgcgcgaa agggatagat acatacggtc accaagcagc aatctctcat ggtggacgga
    14821 caatcgcagt cattggaaca gggatagagc aagtctatcc tgctgaaaat cgtgacttgc
    14881 aagaggtgat tgcaaatgat catcttcttt tgagtgaata tcctcctcaa tccggcccga
    14941 aaaagcattg ttttccaatg agaaatcgga ttatcgcagc tttgtctaaa ggaacatgtg
    15001 tctttgaagc caagaaaaaa agcggctctc tgataacagc acagcaagca ttggatcttg
    15061 gaagaacagt tttttctgtt ccaggaaata ttttaacaga acattctacc ggatgtcatc
    15121 atttgatcca agacggtgca gtttgcacgg tttcgggtga agatatcttg gcagaattta
    15181 aagaataaca ccatactttt catttgcttt tcttgacaaa cgttttctga atggatatga
    15241 ttagctacga tttgtatgaa tcctctttta atagtaggaa cacgacaatg gaaaggagtc
    15301 cattcagtaa tggcgtataa atatttagtg attgtagaat cacctgcaaa agccaagaca
    15361 attgagaaat atcttggacg aaactataaa gtaatggcca gtgttggcca tataagagat
    15421 ttaccgaaaa gtaaaatggg tatcgatttt gaaaacaatt atgaacccca ttatatttct
    15481 atacgcggaa aaggcgatgt catcaaaagc ctgaaagccg cagcaaaaaa agctcaaaaa
    15541 gtttacttgg caagtgaccc ggatagagaa ggagaagcga ttgcttggca tttagcgtac
    15601 ctacttgggt tggatctgaa agaaaaaaat cgggtggtct tcaatgaaat cacaaaagac
    15661 gcagtcaaag cagcttttaa ggaaccaaga acgatcgatg tagatttagt agatgcacag
    15721 caagctcgtc gtaccttaga cagaatcgtt ggttattcga tcagtcctat tctctggcgt
    15781 aaggtcaaga aagggttaag tgcaggacgt gtccaatctg tcgctttaaa aattattatt
    15841 gaccgtgaaa aagagatccg agaatttgtt ccagaagaat attggagcat cgacggtaat
    15901 tttaaaaaag ctcgcaagaa attcaaagca aatttctggg gaatcgacgg taagaaaaag
    15961 aaattaccag atgcacaaag tgtaaaagaa gtcactgcta gaattgatgg aacagacttc
    16021 gaagttaaaa aagtagaaaa gaaagagcgc aaaagaaacc ctgcgaatcc tttcactaca
    16081 agtagcctgc aacaagaagc tgcccgtaag ttaaactttc gaacaagaaa aaccatgatg
    16141 gtagcacagc aattatatga aggaatcact ttgggtaaac aaggaacagt cggcttgatt
    16201 acatacatgc gtactgattc tacccggatt gccgatacag caaaagcaga ggccgcagca
    16261 tttattgaag atacgtatgg aaaagaattt tctagacatg atcaccgaaa gatcaaaaat
    16321 gctcaaggcg cacaagatgc ccatgaagct gtgcgtccaa cgagtgtgct tcgtacacca
    16381 gatgaactca aacaatattt agataaagat caattgaaac tttatacatt gatctggtca
    16441 cgttttgtgg ctagtcagat gacaccagct attttagata caatgaaagt gacattagaa
    16501 caaaatggtg tcatcttcat tgcgaacggt tcaaaaatca aattcaaagg attcatgcaa
    16561 gtgtatgtgg aaggtagaga tgacggcaaa gaagagaaag aaaatattct tccagaactt
    16621 gaagaaggcg atgtagtcca atctgttgat attgaaccaa aacaacattt tactcaacca
    16681 ccagcacgtt ttagtgaggc tacgttgatt cgtgcactag aagaaaacgg ggtagggcga
    16741 ccttccactt atgcaccaac acttgatacg atccaaagac gctactatgt aaaattgaca
    16801 caaaaaagat ttgagccgac tgaactagga gaaattgtca attcattaat ctgtgaattc
    16861 ttccctcaaa tcgtcgatat ccatttcaca gcagaaatgg aaggagattt ggataaaatt
    16921 gaagaaggca cggaagcgtg ggtcaaagtg gttgatcgtt tctataaacc gtttgaaaaa
    16981 gaactgacga atgcggaaga aaaaatcgaa aaaattcaaa tcaaagatga accagcagga
    17041 ttcgattgtg atgtctgtgg acatccgatg gtgataaaat tagggaaata tgggaagttt
    17101 tatgcttgca gtaattttcc tgattgtcgg aatacgaaac caatcgtaaa agaaatcggt
    17161 gtgacttgcc cagtatgtca tgaaggacaa gtcattgaac gaaaatctaa gaaaaacaga
    17221 ctattctatg gttgttcccg tttcccagaa tgtgaattca cttcttggga taaaccagtg
    17281 ggcagaaatt gtccaaaatg tgacggatat ctggtcgaga agaaagtcaa aggcggtaaa
    17341 caagtagtct gtgtaaatgg agattacgaa gaagacatcc aaaaataata gaaaagaggt
    17401 tctttcattg aaaatggtaa atgttgttgg agcagggctt gccggaagtg aagctgcttg
    17461 gcagatcgca caagcaggag tccctgtccg tttatatgaa atgcgtccag tcaaatcgac
    17521 tgaggcgcat cacacaagta attttgctga actagtatgt tccaattcat taagaggaaa
    17581 cagtttggct aatgcagtag ggttattgaa agaagaaatg cgccgattga attcggtagt
    17641 aatcacttcg gcagatgaaa cagcagtgcc agctggtggt gccttagctg ttgaccgcga
    17701 ttctttttct gaaacaatta cccgaaaagt caaagagcat ccattagtaa cagtgataaa
    17761 cgaagaaatt actgaaatac cggaaggaat caccgtagta gcaacaggac ctttgacttc
    17821 tcatccatta gcagaaagca tcaaagcatt caatggttca gatggttttt atttctatga
    17881 tgcagctgca ccaatcgtag acaaagctac gatcgatatg gataaagtat atttgaagtc
    17941 ccgctatgac aaaggagaag cagcttactt gaactgtccg atgacagaag aggaattcaa
    18001 agctttccat gaggcactaa tcaatgccga agtggcagaa cttaaatctt ttgagaaaga
    18061 aaaatatttt gaaggatgta tgccaattga agtaatggca cagcgaggat tcaaaacgat
    18121 gcttttcgga cctatgaaac cagttggatt ggaagatcct aaaactggca aacgtcctta
    18181 tgctgttatc cagctgcgtc aagacaatgc tgcagcttct ttatacaaca tcgtgggctt
    18241 tcagactcac ttgaaatggg gcgagcaaaa acgtgttttc cggatgattc ctgggttaga
    18301 aaatgctgaa ttcgttcgct atggtgtgat gcaccgaaac agctttatga attcgccaga
    18361 attgctgaaa ccgacttacc aatcaacgaa acgcgaagat ctgttctttg caggtcagat
    18421 gactggtgta gaaggctatg ttgaaagtgc agcgagcgga ctacttgctg gaatcaacgc
    18481 cgcacgctta gcaaaagaaa tggaaccagt cgagtttcca caagaaacag ctattggaag
    18541 tatggcttac tacattactc atgcggaagg aaaacatttc caaccgatga atgccaacta
    18601 tggtctgttt cctgaactgc cagagcgcat tcgagacaaa aaagcaagat atgaagcaat
    18661 tgcacaacga gctttaaaag ctaatgaggc aataaaaaca gaattagaaa aaactgcaat
    18721 cccaaatgag taaggaaact ccatgaagag ggtgagacgg atagtctcgc tctttttctt
    18781 gcgtttttct taagaatttc tcaaacaaaa tgtgaggaat tgcgccaatt gtgaacaaac
    18841 ttagaaatat tggttaaatt ctgacaattt atttgtcttt ttataaacgc tgtgctaaat
    18901 tattctctgg gacaaaagga aggtgagaaa atgacagaag ccacgtggga acaaaaattt
    18961 ttgcgttatt tgattgtcga aagaggatat tcagaaaaaa cgaaagaagc atatgaagaa
    19021 gacattctcc atttcaaaca ttttttggag gaatctggag acgctgatct gctgaaggcg
    19081 gaatatttta cggttcgtgt ctatttgagt gcattagctg atcatcgtcc tagctatagc
    19141 agaaattcta tcagtcgcaa gatagccagc ctacgttcgt tttatcagta tcttctcaaa
    19201 gaagaagtga taaaagaaaa tccattttct tacgttcatc tcaaaaagaa aaatttgaga
    19261 ctgccacgtt ttttttatga aaatgaaatg caagcacttt ttgacagcgt agcaggagat
    19321 agtcctttgg atttgaggaa ccgcgcttta ctggaagtcc tttacgggtc aggcatccgt
    19381 ttaagtgagt gtagcagttt gtcggtatca gacattgatt ttgattcaga ggtcatgttg
    19441 atccatggta aagggaacaa agagagatat gctcctctag gttcttttgc tcaagatgct
    19501 ctgcaagaat atttcaggga aggccgccag gtattgatga caaaatatca cgaagagcat
    19561 gatatcgttt ttgtgaatca tcatggaaaa ccaatcacgc ctactggaat tgaatatgtc
    19621 ttgaatcaaa tgatcaaaaa aagcagtttg gatagtgata tccatcctca catgctgcgc
    19681 catacatttg ctactcattt attaaataat ggggcagatt tgcggacagt ccaagaattg
    19741 ctaggacatg ccaatttatc gactacccag atctatgccc atgtaacaaa agaaagttta
    19801 caaaaaaact atcgttcttt ccatccaaga gcgtaaaaat aaggaaaaga acaaatttag
    19861 gaggaaacaa acatggtcga atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa
    19921 gacgggaaat ttgctatggc tggagatggc caagtaacaa tgggtgaatc tgttgtcatg
    19981 aaaggcactg caaagaaagt acgacgtatc tacaatggag aagttgttgt aggtttcgcc
    20041 ggaagtgttg cagatgcgtt tactttagaa gaaaaatttg aaggcaaact aaacgaatat
    20101 aacgggaatc tacaacgcgc agctgtcgaa ttagctcaag aatggcggac acagcaatct
    20161 atgcaaaaac ttgaagcgat gctgatcgta atgaatgata aagaaatgct tcttgtttca
    20221 ggtacaggag aagttatcac accagatgat ggtatcttag ctatcggatc aggagggaat
    20281 tttgcgttgg ctgcagcccg agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa
    20341 gaaattgcag aaaatgcgtt aaatattgcc gcagatatct gtatctttac taatcataat
    20401 attatcgtag aagaactgta agaggtgagt agatatgtat gaattaaaac aaacaccaag
    20461 acaaatcgta agtgaattag atgaatatat cgtaggacag cacgcagcaa aaaaatctgt
    20521 tgccgttgct ttgcgtaacc gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga
    20581 tattacacca aaaaatatgc tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc
    20641 acgacgtttg gcaaaaatcg tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac
    20701 tgaagtcggt tatgtcggaa gagatgtaga atcaatggtt cgggatttag tagaaaatgc
    20761 gatccaaatc gtagaaaaag agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc
    20821 agaaaaacgt ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac
    20881 taatcaattt gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc
    20941 gcaagaagaa gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca
    21001 aaagggttta ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc
    21061 accaatgatg aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc
    21121 tttgacaccg aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt
    21181 gatcaaggaa gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa
    21241 attagcggaa gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc
    21301 acagcaatct ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga
    21361 aggttcacaa gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc
    21421 ttcaggagct ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt
    21481 cccgatacgg gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga
    21541 accaaataat gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt
    21601 tacgtttaca aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga
    21661 cacagataat atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct
    21721 attatttgaa gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga
    21781 tgacaaactt ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat
    21841 cggaggacga caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag
    21901 caaaagaata cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta
    21961 ggtggtattc ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat
    22021 aatgaacgtc acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt
    22081 cctgaatctt atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt
    22141 actagtgata ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg
    22201 actgtcgttc cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg
    22261 gaacaatcgt ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt
    22321 atgcagatcc tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct
    22381 gtccaaatgg caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt
    22441 gaagcattgg atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc
    22501 atcactcgtt ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa
    22561 tctcgttcgt taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac
    22621 gagttagcaa aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc
    22681 cagccttccc attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt
    22741 caaaatgacc agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa
    22801 tcaaaagaac atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat
    22861 gcaccggttt tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg
    22921 aaaacatatc aaatggggca acacggattt gctcgggata tggattttga agtcattgaa
    22981 catggacaag aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat
    23041 ccatttgatt ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat
    23101 tatcaagtgg caaataccgg tgaaaaagaa atgttctttt ctatcggtgg acatcctgct
    23161 ttcaatgtac ctcttgaaga aggattgtca tttgaagatt tctatctggc attttctcca
    23221 ttgaaatcaa gattacgcct accattgaaa ggcccattca tcgatatgga tcaaaagacg
    23281 ttaggtcaga caaatacgaa tctggctttg aatcgtgaac tttttgagca agatgccttg
    23341 gtttttgaaa caaggggtct aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc
    23401 gtaactttaa gctataaaga tatgccttat gtagggatct ggtctcctta tccaaaagaa
    23461 gcgcctttcg tttgtatcga accgtggtgc ggaatcgctg acacggtgaa cagtagtgga
    23521 aacttgatgg aaaaagtagg gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa
    23581 tattccatca cagtgaagta agaggttgtg accgagaagt tagcagggaa caccgtttac
    23641 caaggtgtgt aaaaaagcta tcctgtaagt aataagggag tgtgacatcg atttgtggct
    23701 ctttgtcaat aaggactgat gagttgtgca aaat
//
LOCUS       DACEPD010000233        18568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.guided.059, whole genome shotgun sequence.
ACCESSION   DACEPD010000233 DACEPD010000000
VERSION     DACEPD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.guided.059"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(<1..116)
                     /locus_tag="IW797_002865"
     CDS             complement(<1..116)
                     /locus_tag="IW797_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ1225579.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHP"
     gene            202..543
                     /locus_tag="IW797_002866"
     CDS             202..543
                     /locus_tag="IW797_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ1225580.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(714..1562)
                     /locus_tag="IW797_002867"
     CDS             complement(714..1562)
                     /locus_tag="IW797_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ1225581.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            2160..3023
                     /locus_tag="IW797_002868"
     CDS             2160..3023
                     /locus_tag="IW797_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1225582.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            3123..3605
                     /locus_tag="IW797_002869"
     CDS             3123..3605
                     /locus_tag="IW797_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ1225583.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(3868..4335)
                     /locus_tag="IW797_002870"
     CDS             complement(3868..4335)
                     /locus_tag="IW797_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ1225584.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            4458..5309
                     /locus_tag="IW797_002871"
     CDS             4458..5309
                     /locus_tag="IW797_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1225585.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(5560..7518)
                     /locus_tag="IW797_002872"
     CDS             complement(5560..7518)
                     /locus_tag="IW797_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1225586.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(7515..8387)
                     /locus_tag="IW797_002873"
     CDS             complement(7515..8387)
                     /locus_tag="IW797_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ1225587.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(8729..9601)
                     /locus_tag="IW797_002874"
     CDS             complement(8729..9601)
                     /locus_tag="IW797_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ1225588.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            9729..10298
                     /locus_tag="IW797_002875"
     CDS             9729..10298
                     /locus_tag="IW797_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ1225589.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            10365..11102
                     /locus_tag="IW797_002876"
     CDS             10365..11102
                     /locus_tag="IW797_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ1225590.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            11333..12601
                     /locus_tag="IW797_002877"
     CDS             11333..12601
                     /locus_tag="IW797_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ1225591.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            13088..13552
                     /locus_tag="IW797_002878"
     CDS             13088..13552
                     /locus_tag="IW797_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ1225592.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            13536..14165
                     /locus_tag="IW797_002879"
     CDS             13536..14165
                     /locus_tag="IW797_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ1225593.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            14519..15997
                     /gene="msr"
                     /locus_tag="IW797_002880"
     CDS             14519..15997
                     /gene="msr"
                     /locus_tag="IW797_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ1225594.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            16044..16490
                     /locus_tag="IW797_002881"
     CDS             16044..16490
                     /locus_tag="IW797_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ1225595.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(16689..17204)
                     /locus_tag="IW797_002882"
     CDS             complement(16689..17204)
                     /locus_tag="IW797_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ1225596.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            17321..18522
                     /locus_tag="IW797_002883"
                     /pseudo
     CDS             17321..18522
                     /locus_tag="IW797_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         5837 a         3288 c         3823 g         5620 t
ORIGIN      
        1 ggatgaaatc cggcagcttt cccaacgata ccaaatgcaa gtccaatacc gatataaccg
       61 aaaacggtag gtaacgtgtc tttgatcgca gtttttatat ctaacttttc atccaataac
      121 aaaactcctt tagcacgaca taaattttca taattgtcgt taaacttgtg aatattctaa
      181 atttttaatg agcatttgtc aatggtggga ttgaaacgta aagaactagc gggtcgcaga
      241 tttacacgta ttgtttcggt gggaggaggc gcgaaaaatc ttgcatggct acaaatccaa
      301 gctgatattt tcgatgcaac ggtctattgt ttaagtatgg aggaaggacc tgcattaggc
      361 gcaacaatga ttgctgcact agggttaggg tggttcgaca cacctgaaag ttgttgtcag
      421 aattttatcc aatatacaga tgcagcggaa cctctcttac aaaatgtaga aaagtatcat
      481 aaaatctatc aacaatgccg gaaaatctat ccagcaacga aggaaatttg tcatgactta
      541 taaaacaaaa cgcgtaccta atagtcaaat gactaatatt gggtacgcgt taagtcttct
      601 ttacttcatc ctttgcatga ttcgtgtttt taaagcaagg tctgctgtat ttcttgtgga
      661 tttaggatgg ttgttatcgt ttacttgcta tttttcattg aaatcgtctt tctttaccgt
      721 ttctttagtt ttttatctat ttttttcttt tggttagaaa attctttttc ccgaaagtct
      781 tctttcagga tttttataga gtagcgtttt gaataagagg cgtcagtatg atcgacttgg
      841 cagacgaacc aagagaacat ttgccttttt tctgcatgag gtgcttctgc taataattct
      901 ttccataaat aataacagtc ctctatcaga agaagggttc gttcatcctc aggctcccaa
      961 tagaatgttt cgagagtagc aatcacttca ttgatcaatt cgaatgcggg aagataggca
     1021 ttttgctgaa taagcgattc aactgcttct tggatgaact ttcgcatctc catgatgaac
     1081 ttcgctgttt tcctgttttt gtttttcttg ataatatgat cgtatttatg gatgatagtt
     1141 gttaatttat ctgaataatt tattgcatga aatggcatct taactttgaa gcgttcgata
     1201 aagtgcggat gttctataag aatctctgtt aaaagctccc gtacaagaga ggaactagcg
     1261 gaggaaacaa gggacgagag ttcggaaagt gagtagttgg atttttttat tggacgttct
     1321 tcgtaagcga ataagacagc agccatgtgt ttgcattttt ttccttctat ggcatataga
     1381 caatcacaag tcatgttggt cttttcatcc caagtgatca ctaccttata atcttccgtg
     1441 ccagaaactg ttgcggacaa gtacttgctt gtatagttca aatgacggac aaaaccgtgc
     1501 agatgatagc tgtaaccgcg ttccaaaacg ttttcaggaa aatactcaag ccaattattc
     1561 atctagttct cctttcatga aacacttatt tatatttagt ataaagaaac gtttaagaaa
     1621 aaagtagctg attttacata tttgataatc acttttgctc gaattgtcaa aaattcattc
     1681 tccttttctt ttcttagaga atgtgagaaa aagtaaaaga atagcgtgtt ttaaagatgg
     1741 aacttttttt gttttttatt tatctgacaa aaatgaagtg attttgttag aaataaataa
     1801 ttgttccaat tgataaaaga ggtatgatgt atcgcaaaga aaaatccaag cagcgttctg
     1861 atcagcagtt tgaacaacaa tttgctttgt acagtaggta gtaagcggat taactaaggg
     1921 aataaaagat atttttaccg ctttgtttac ttagaatctg tcttccccaa aatttatttt
     1981 tctaaaggta aaacatagat aggcagataa taccaattat ccgttacgaa acgctgcttg
     2041 agaattttct ttagatccat acaacatttt ctacatctga ctaaattggt tcctgattca
     2101 tcaagtaatt gactttcttt gataatatga taaaaaggca atcaaggagg aagataaata
     2161 tgcttaaaga tctgaaccaa ttgatcgatt acatcgaaga acatttgaca gaaggacttt
     2221 cctataaaat aatagccgaa aatacaggaa tctctgaata tcaattaaaa cgcatcttta
     2281 cgtttattgc agggatgtcg ctaatggact atatcaaaaa ccgtagattg gctttggcta
     2341 atcgcgatct tctagatgga tcaagagtga cagaaacggc gtttagatat ggatatgaaa
     2401 cagtcgaagg tttttcaaga gcttttcgtg attggtcagg atatctgcct tcagaaatca
     2461 gtagatacgg catacaaaaa tcttttccac gcttgacatt ttttattgat gtcagaggag
     2521 gaaaatcgat ggaatttcgt atcgaaaaga aagaacagtt ttatgtggca ggtgtggcga
     2581 aaaaagttcc tgtcaaattt gaaggagtca atcaagaaat acaaaagtta gcgcagacga
     2641 tcacggagac tcaaagggaa gagatgcata agttaggaga tttatatcca aatcaagtaa
     2701 tcaacgtttc ttatggattt gatggagacc gcttggaaga aaagggcagt ttgatgcaca
     2761 tgattggctt tgctacgaca aaagaaaatc cgtacgctga tttagaaatg cttgctatag
     2821 aagaacacac ttgggccatt tttcctaata aagggccatt tccccaaact ttacaagata
     2881 cttgggggaa aatctatgca gaatggcttc cttcttcaga ttatgggtta gtcgaagctc
     2941 ccgagatctc ttttacaaaa tggggagagg atttcagtaa tgtttacagt gagatttgga
     3001 ttgcagtgaa agcaaaaaaa tagttaaaaa ataaaaatat ccctagaaaa aagagcgtaa
     3061 tcgtgtcatt tccctttaat tttatgtaag atgaacttaa tataatagaa agggtgggag
     3121 aaatggcaaa gtataggatc gaagaaaaac cagattttac ggtgacgggt attggtgtgg
     3181 aattgaagag tagcaatacg gattttgtcg gtttggctgc agagaaacaa gcatttgagc
     3241 aaaaagttat cggtaatgga acaatagatg cattgaaaca agctgctgaa aatcattatt
     3301 tctttacagt caacgaaccc tataacggga aaatgatgta ctatgtcggt gttcaaacga
     3361 gtcaggatgt tccaaacact acgcgaatca tcccattccc aaaaggaacg tatcttgttg
     3421 taagcgcttt agcagaatcg cctgaagaat taagaaatga tttgacgaat acggcctttg
     3481 ggcatgtttt aagagaagta gaagattacg cctatgttgg gggtccaaat gctgtagtcg
     3541 aaatgggagt taccaacggg aaatatatgg gagaaatctg gataccggta gtcaataaac
     3601 catgataata tagaggtcgt gaaagtagcg ctttgacctg agcattagag aggaaaaatg
     3661 ggaaatagct ttccatattt tgcatttttc agaactaatg ccgagggtca gctacttgaa
     3721 caccgtttat caagacgtca tgacttcagc gctttgacct gagcattaga gaggaaaaag
     3781 gggcatgaca aggcttttgt cccccggaga ataaaagaat ttatgtagac gattccgatc
     3841 agaaaaatgc aggcacagta gagagaatta taaaaatctc tctacgaggc ctgtgttttc
     3901 tggtcttttt tctttttcca aaacggctat tttatgtatc agactatcaa gtgtgatctt
     3961 agccatttct tcttctgcct ttttctgcag ccgagcgtag ctttcttcta aagcttcttg
     4021 gatattcgca ccaacgacgc aatttggatt tgtttttgga tcgacttgga taagctgtgt
     4081 attaccttcg atgctgcgat agacatctag taaagaaatc tgatctggac ttttggcaag
     4141 ggaaggctgc ggttttccgg tttttgtaaa gatcaaaccg gattttttga gctgactcat
     4201 gattttacgt acatttgctg catttgtttc gatactactg gcaatcatct cactagaaag
     4261 tgatcgggta tcttgaaaca tttcaatata ggcaagtaca tggattgcat cactaaattg
     4321 cagagaatac ttcatcagtt gtcatccttt catttttttt gcttgactta tcttaccaca
     4381 ctctttatga taaaggtgta atttataaat gtacagctgt aaatcttaac aaataaaaag
     4441 aatagggagt ggcaaatatg cattatttag taacaggagc aactggaggt tttggtggtt
     4501 atgcgctgga attcttaaaa aagatggttc cacaaaatga aatctatgta ctagtacgta
     4561 gtgaagaaaa aggagaagca ctgaaagaag ctggatttaa tatacgtatt ggtgattatg
     4621 atgatttaga ctcaatgaaa gaagcgttga aaggaataga tcgattgctt tttgtctctg
     4681 gcgttccagg aaatcgtcaa gccgaacata agaatgtagt gaaggcagca caagcagctg
     4741 gggtatctta tatcgcttat acaagttttg cagatgccga tcactcgatc agtgtattag
     4801 cgccagatca ccaattcact gaaaaaatga ttaaggattc aggtatttcc catacatttt
     4861 tacggaataa ttggtatgta gagaatgaat tacctatcat tgggcaggct ttaaaaactg
     4921 gtcaattcgt ttatgctgca ggaaatggaa aaacaggttg ggcattgaaa cgtgaatatg
     4981 cagaagctgc agcaaaagta ctagtagaaa gtgactctcc agaaatctta gaattatccg
     5041 gcaatctaac tgactatgaa gaattagcca aggcattaga gcgtgcgaca ggtaaagagt
     5101 tagaaattat tgaagcaagt gatgcagcgt ttgtcgaaaa tctaaaagaa gccggattcc
     5161 ctcaagaagc agctgacatg tttttagcga tccaacatga tattaaaaag gatcagttag
     5221 ctatttcctc tgatgattta gaaaaagtat tgggaaaacc attaacttct cttgaagcag
     5281 cattaaaaga aatattggat caggaataat gtataaagag gttgtgaaaa agccgattag
     5341 ttttgagtat gaagaaaaat tttgtgaaaa agctgttctg catcaagtaa taagaagagt
     5401 caaataaaaa atagctcttc atatgtttcg tttagctgga cttattaccg cggagttggc
     5461 aggagaacgc cgtttttatt cggaaaaagg agctgagaca agatttttgt ctcagctcct
     5521 gtttgtttat cgataaaaaa gtctggggaa gacgatggct caggctttgt tggaaacaga
     5581 gccgatttta tcatttgctt ttacatcacg tgcgggttcg atttctaagc tttggacttt
     5641 gtcgccattt gtgaatacaa cgaccatatc cgtgtttttg cctgcagatt gaatagcggc
     5701 aagatcaact aaagcaatca attgacctct tgcgactttt tgaccttctt ctacatataa
     5761 agtgaatggt tcgcctttta agtcaacagt gttgatgccc atatgaagca agacttcgat
     5821 accagcatct gtttggattc cgacagcatg tttcgttggg aaaatgtttg tgatcgtacc
     5881 agcgattgga gagaagattt ctccattttc tggtaataca gcatagccgt cacccatcat
     5941 tttttccgcg aatacatcat ctgaaacttc agtgattgga atcagtttcc catttgcgat
     6001 agcgtaaata tcttgttcga tcgtttcacc ggcaaatgtt tctgttttaa ccacggtgtt
     6061 tgtttgtgca ggtcgtgctg cagcaccacc atgtagtttt gccatctcat ctgcaacgaa
     6121 ttgtacttct gtacctacga tgacttgcgc attttttccg tcgatgatct tcataccagg
     6181 tacgccagtg gctttgattt tattttgatc gatcaggcct gtatctttga cttgcaaacg
     6241 aaggcgtgta gtacagttat cgatcgaagt tacattgtcg gctccgccta atgcagcgta
     6301 gattttgctt gcttgtgccg catatttatt gtctgaatta gtcgttgcag tatcgtcatc
     6361 tgaaagatct gcttcctcgc ggccaggagt catcagatgg aattttttga tagcaaagtt
     6421 aaagccaaag tagtaaatca ctgctgttac taatccttgg acgatcaaca tcaatggctg
     6481 attcgcaatc ggatttttca aagataggaa gtaatcgaca aatccggcac taaaggcaaa
     6541 tcctgctgtc caatggaaga atgcactcac tgctaaagaa atcccagtga aaattgcatg
     6601 caagacatat aatggccaag caacgaacat aaatgaaaat tcaagtggtt ctgtcacacc
     6661 agtaaagaat gaagcaaaag ctgctgctag catcaaggaa gctgtttttg ctttgttttc
     6721 tggacgtgca tttctgtaaa tagcatatgc tcctgctgga agcccgaaca tcatgattgg
     6781 gaaaaatcca gcttgataca ttcctgttac gccttttgtt ccttcagaag ccaaaaattt
     6841 accaatatca ttgattcctg ctacgtcaaa ccagaacacg gagttcattg cttgatgcaa
     6901 tcctgtaggg atcagtaaac ggttgaagaa gccataaaga cctgcaccac cccagcctaa
     6961 actcaaaatg aatttagcaa aagaaactaa agcagagtaa accggaggcc atacgagcag
     7021 tagcactgca ctgatcagca acatgacaaa ggcagccaag attggcacta gacgcttgcc
     7081 gctaaagaaa gataaagcca tcggtaattt tgcttcgtgg aaacgattat aaagagctgc
     7141 cgcaatcaat ccggcaataa taccaataaa tacgttactg atctgagcaa aagatggatc
     7201 aactgattct ggtttgatat tcaataaagt agctactgaa tcagttttta aaacatttac
     7261 tggaacttca aatgcgacca aacctgccaa ggcagcagca cctgatttgt ccactgacat
     7321 ccctagtgct aacccaacgg caaacagcaa aggtaattga cctaaaactg cgttaccgcc
     7381 agcttgtaaa aaatgagcga caatgtcgtt actataagat gcccaccagt ttccgatccc
     7441 tactagtaaa gcagcagcag gtaagaccgc aactggaagt tgaagggaac gtcccattcg
     7501 ttgtagatat gctttcaatt tttttcctcc tcatgattgt taacatttgt ttgtacgatt
     7561 gcgcttctta aacgctcgat atggatggcc aagtaaccca gttcttcttt cgtgatcgcg
     7621 agctggtatt ctttggtcag caacacacgg atttttttag aaatcttata gctttctggg
     7681 tattttcgtt tgaccatttc tacgatttcc gtgtctaaaa cagcatattg ttgcgcatgg
     7741 gttcgttgta gaagcagtcg taaatgattc accagtcttg aataattcag tgaaaatggt
     7801 tctgaatgga tatctgtttc caattctgat tccactaaat ggatgatgtc agatacaatc
     7861 gtcacttcac ggatactgcg atggttattg ttccgaccgc ttcgtccgct gtgaatatgg
     7921 attgcgatat atcctgcttc gtcataagta tatggaatcc caagctcttt tgtcagatac
     7981 tctactgccc attgagcaat actgaattca tcactgtaaa gcacttcgat ttctcttaaa
     8041 agtttgttgc ggatgacgat accgttatta atgttctccg cagaaaaagc aatgtggtcg
     8101 gtcaaagcga tcaatacatg ttcattcaac ttttccatca aaacagtttc cgcatgatca
     8161 atgatcgttt ctgctgcaaa taaaaagcgt tcatcaatct gattaagtaa attttgcaac
     8221 ttgatttgac cttctggttc catgacaaat atccgttcga catcacgatc aaaaatcaaa
     8281 tcatttcttt ttctgtcaaa tccaatccct tttccaatag ctactttttc ctgtccaccg
     8341 tcatctacca atactgcatt ttgattaaag atctttttga ttttcatgcg cgcacccttt
     8401 ttgaagattt ggttttcacg tttgctagac cagttgattg cggccaaaca aaaatagaca
     8461 cgaaaaacct cctagggaaa ccatgttcct cagagatttt tcatgcctag tatgatctag
     8521 tcacatgaaa cgaattattg ttaacgtaat caattttata tagacagtta tgttttgtca
     8581 attctttttt tataattata gtaatttact ggaattatca gaaataaaag gaagaaataa
     8641 attaagaaac ttatgttatt taatcgttgg atcttttgct tatcttattc gttgttaaaa
     8701 agctgaaagc gaaggaaaaa aactctactt aactgtattt ctccgttagg acatctaaaa
     8761 atttacgagc agcaggagaa agaggctggt cttttttcca aatgagattc agtgaagctt
     8821 gtaatggcgg atcgagtggg atgaatattt tttccgtccc ataagtattg atgatcccat
     8881 ctagagctaa tgcaccaaaa gtcccgttat cgaccatcaa agaagcattg tacaataagt
     8941 tatatgttcc gacgatcgat aactgatcta tgctgaaacc tagccattca gctagttgac
     9001 tgtccactga cgtttgccgg gaaatgatca aaggaacagt tttcagttcc tgcggatgga
     9061 tgacggtttg ctttgctaaa ggatgatttc gatgtatcaa aagtccccaa cgatcttggg
     9121 ctggcagtcg aaatgactgg tatttttgtt tgtttgtcgg ttcgattacg agtccaaaat
     9181 caagtaatcc gctatccagt ttgtctatga tatcatctgc attcccgcta tataaatgaa
     9241 actggatatc aggatgcgcc ttgatcaacg aatgaaggct atcagaaatg aaagagattg
     9301 ctttagtttc gcctccgcca atgtagattt ctccagagat gattgcgtca tttgttaaat
     9361 tgttcgccgt tttttctacc aacgacaaga tttcttttcc ttttgaataa agatatcgac
     9421 cagaatccgt caactgtatc tctcgggagc cccgagtgaa aagtgtcaca ccaagttctt
     9481 cttctaaatc tttcagttgt tttgagagag taggttgcgt gatatgcaga gattttgctg
     9541 ctcggctgat atttttttct ctagctactg ctaaaaaata cgtcagtata cgaatatcca
     9601 ttttttccct cctgttttta aaaaagcata ccactaagta tagttaaaag gaatagttaa
     9661 gtatgtttta taagtattag acatgataag ccgtccgtta taaagtaagg ataaaaggag
     9721 ggagcaaaat gacaagcctc atcgaaaaac aaatcgtcaa taaagagatt cgagtaacgg
     9781 atccgctctt tgaagtgatc catcagatcc aagctgaaaa cgaagagcct ttagcagcgc
     9841 tgaatactgg gtaccatgaa ccagcagata tccgcaaacg gctagaaaat atcattagtg
     9901 acaaagtaga tgatacggtg actgttctgc tgccttttta tacagatttc ggcaaacata
     9961 ttagtatagg gaaaaatgtc tttatcaatc gtcaagtgat gttcgttgac ttaggtggca
    10021 tctgtttaga agattcagta ttgattggtc ctcgtgtcaa cctcatcacg gtgaaccatc
    10081 taaccgatcc aaaagaacga agaggcttgt cagtaaagcc aatccacatc aagaaaaatg
    10141 cttggatcgg agcaggagca actattctgc caggcgtaac gattggcgag aatgccattg
    10201 ttgcagcgaa tgcaactgtt acgaaagatg tcccagacaa taccatcgtt gcaggaatcc
    10261 ctgccaaaat cgtcaaacct gtcgaacgga ttatttgaac agtaagatga aataagagat
    10321 agtgagtata agcaagagat ttttcaaaac aggaggagaa tagaatgaat gatttatcag
    10381 aaaaagtgat tgtcatcatg ggcgcttcaa gcggaatcgg tgaagcaaca acgaagctat
    10441 tagcagaaaa aggggctaaa ctagttatag ctgcacgccg agaagatcgc ctaaaagcaa
    10501 taaaagaatc tttaccagaa gctgagctat atattcaaac agcagatgta agagattttg
    10561 cacaagtcca agcagttatc gatttagcga tggaaaagtt cggtcgaatc gatgtattgt
    10621 acaataatgc aggaatcatg ccaaccgctc cattagttga aggtcaccgt gatgaatggc
    10681 aaaatatgct tgatatcaac atcatgggtg tgttaaatgg aatttccgcc gtacttccaa
    10741 tcatggaaaa acaaaaatct gggcatatca tttcaactga ttctgtggcg ggacatgttg
    10801 tttatcctga ttccgccgtt tattgcggaa caaaatttgc tgtccgtgca atcatggaag
    10861 gattacgtca agagcaacgg caaaataaca tcaaatctac gatcatctct ccaggagctg
    10921 ttcaaactga attatatcaa accatcagcg acaaaaaggc tgctcttgaa cttcatgaag
    10981 cgcaaaaaga atggggattg acctccgaag atatagcttc tgctgtagca tttgcgatag
    11041 aaacgccaga ccggatgtct gtcagtaaca tgattatccg tccgacgaca caggaagtat
    11101 aatccataac ggataatatc caaagagaat tacttgttaa atagttattc aaacgaccat
    11161 caatttaagg cgtgaatgga gaagaatatt cttcatttac gtctttttat gtagaaaaag
    11221 caaatttatc aaaaagaaac aaatgttatt taaatatgga cattgacttg cttttacttt
    11281 tttagttaca ctgttattta ggaaaagttt cataattcaa aggagaaaaa agatgaaagc
    11341 aaagaattta ttatttagcg ttattctttt aggaaatcta gcagttgcca acacaacagt
    11401 gctagctgaa gaatcgacag aaactgtcgc aactcagaca tcgagttctt cagaaacagg
    11461 aattgaaaac gatacagaga gtactttgca gtcagctact gtctcagaaa atacaatttc
    11521 aaccgaagaa ccaacaactg aaacaacaga acaaacacaa accgaagaca gtacatcagc
    11581 agaagctcag aacaatccag agaatgcaga agaaatcacg aaccaattga cagatgggca
    11641 aacattgaca catacccatc aagcagattt gtctgaattc gaaaactata ctggctatgc
    11701 tttttatgct cgctcatctg ctaattctca acaagcgttc attgatagta ttgcctcaac
    11761 tgcacaaagt ttggcaagtg ccaatgactt atatgcatca gtcatgattg ctcaagccat
    11821 cgttgaaagt ggctggggga acagtacttt ggcttctgcc cctaattaca atctatttgg
    11881 gattaaagga agctataatg gccaatctgt tacgatgcct acttcggaat atgtgaatgg
    11941 gcaatggatt acggtgaatg ccgcttttag aaaatatcct tcgtacaaag aatcattgca
    12001 agataacgta acggtattga agacgacttc ttttcagcca ggcgtttact actattccgg
    12061 tgcatggaaa agcaatacga actcttacaa agatgctacg gcttggctaa caggtcgtta
    12121 tgccactgcg cctaattatg gttctacatt gaacaatgta atcgaaacct ataatctgac
    12181 acagtatgat acagcaccaa cttcaacttc tccgatgtat cgtctgtata accgtcatac
    12241 aggagaacat ttatatacat tgaatgccgg agaaaaaaat tatctgccaa ctgtaggctg
    12301 ggaatatgaa gggattgctt ggcaagcacc aaacagcgga caacctgtct accgtttata
    12361 caatccttac agtggagacc atcattacac aatggcacaa tctgagatca actctttaac
    12421 aaaaatcggt tggcgttatg aaggtctaag tttttattct ggtggttcca agccaattta
    12481 tcgactattt aatccaaatg aaaaaactgg tacccatcac tataccttga gtgctaagga
    12541 aagagattgg ctaacaccga tgggctggag atatgaagga ataggatttt atggttatta
    12601 aaaagaggta gtgaaaaagc tgccctgcat caagtagtaa gaacagccaa acaaaaacag
    12661 attttcatgt tttttgtttg gttgaactta ttaccgcaga tgcaagtttt tgaacaccgt
    12721 ttactcaaaa aaggttgtga caacatcttg tcataacttt tttattactg tttaagcata
    12781 ctgatccaat ttctaatatt cgacgggatt cttgcaaaag gcgatttcgg aaatttttgg
    12841 cggtgtaaaa ttttctactg cagaagagct atcttctata gtttgataag aatcaataat
    12901 cttggtttta gcgttgtgct ttctttatca ttttttccgt tcattctaca aaaaatttct
    12961 tttctctata cttttctatt gagagcgttt tttttattac acagctgatt ctttgacttt
    13021 tttctttttt tattctatta ttgacaagtc aatcgttgac gagtcaatta ttagaaagga
    13081 ggaaattatg tctttagaag agattagtca ccttttatat caaatcaaat tagccgatca
    13141 aaaaatcgtc cagatctttg aagaaaatat cggtataagt cttactagat acgagatcat
    13201 gatgagactg aaagaagaaa atccattgct acaaagcagt ttgcaagaaa agttgcagat
    13261 tgatcaagga gcagttactc gtcatctgaa ggtattggaa gcaaatggct atgtttcgcg
    13321 tcagagaaat cctcagaaca accgggaagt gtatgtagcg ctaacagaaa aagcccagaa
    13381 gaaaatcgag cattgtgaaa tggaccataa agatacaggt tccgtgctat ccccggcttt
    13441 cagcgatgta gaaatgaaac aatttctaac acttttagat aaattgaata cccaactttg
    13501 caatataaca acagaaacga aagaggttaa taataatgga acaaaaaaat gatttttcaa
    13561 caattatgac tggtagaaga tccgttcgcg tatatgatga aacatacaaa atcccacacg
    13621 aagaaatgct ggaaatgatc aaagaagcga cgactgcacc ttcttctgtc aatatgcaac
    13681 catggcgctt tgtggtcgct gaaagcgagg aagcaaaaga aatcttgcgt cctttgatcc
    13741 gtttcaatac gagacaaaat gatacttctt ctgcaatggt catgattttt ggcgatatgc
    13801 aatgttacga atatggcgaa gaaatctata accaagctta cgaatcaggg aagatgccaa
    13861 aagaagtcag agatcaacaa ttagctgcga tcattcctta ttacaagagt ttttcaagag
    13921 aagaaatgaa cgatgtagtc aaagtcgatt ctagcttagc ggctatgcaa ttcatgttag
    13981 ttgcgcgtga tcatggctat gaaacaaatc caattggtgg attcgaagca gatcagttgg
    14041 cagaggcatt tggtttggat aaagaccgtt atgttccagt tattatttta tcaataggaa
    14101 aagcggtaga agaaggttac gagtctgttc gtttagcacc agaaaagatc actacgttcg
    14161 aataaaaggt tttcagttac tattcagtag ctattgcttt ttttgaatcg tgtgtggtaa
    14221 cctaataaaa aaggaggtcg gaaaaatgac tgcatcgatg aaattacgtt tcgaactttt
    14281 gaataacaac taaataaatt caaaagacat gtataaacaa caatttcatg gatggagtat
    14341 gtccattttt tcgaacgaca gtagaatagg tgaaagctat gacagatttg gtgatttagt
    14401 catagttatt tcgttcagag taggaaaaga tgtgatgcag tttttttgca aacagatttt
    14461 tctgctcttt tttagtatcg ataattgttg ttttacatga aataggagtg taaaacatat
    14521 ggaaaattta gcagtaaata taacaaatct acaagtcagc tttggcaacc agctagaact
    14581 atctattgat tctcttcgtg tctatcagca agatcgtata gggatcatag gagaaaatgg
    14641 tgtcggtaaa tcgactttgc tcaaactaat agccggtgaa ctttttcccg atcatgggaa
    14701 aatccaaaca gagatcacct tcaactacct gcctcaatta acctatctcg ctgaagcaaa
    14761 ggacctaaat ttggaattag ccagtcattt ccagttaaga ctggaagaaa cttcagagcg
    14821 gaaatggagc ggaggggaag aacgaaagat cgagttgata cgtcttcttt cttcttatga
    14881 acaagggatg cttctagacg agccaacaac ccatctagat agaaaaagta ttgatcgact
    14941 gattgaagag cttcgttatt attatggtac gctggttttt gttagtcatg atcgctattt
    15001 tctagatgag ctagcatcga aaatctggga agtaaaagac ggagaaatcc gagagttttc
    15061 ggggaattat agtgcctatc tcactcaaaa ggaattggag aaaaagactc agctacgaga
    15121 agcagagtcg atcatgaaag agaaaaaacg attggaaaaa tcgatccaag aaaagaaaaa
    15181 acaagcggaa aagttggaaa aagtgtccag taaaaagaaa aagcaacaaa tcagaccgga
    15241 tcggttgtct tcctctaaac aaaaagacag tgtacaaaaa gccatccaaa agaatgcgaa
    15301 aacattagag agaagactcc aaaaaatagg agaaacaacc aagccgcagc agatgaaaca
    15361 aatccgtttt ccagtaccaa aatctcttga actccacagc cgctatccca tcatgggaca
    15421 aaatgtccaa ttggaaagaa gtgggagaac attactggta aatggtgatt ttcagttttc
    15481 tttaggtaaa aaaatcgcga ttgtcggtga aaatggttca ggtaagacaa ccttattaga
    15541 acatatccgc aaacaaggag aaggaatcct tctctctccg aaagtaagct ttcaagtata
    15601 tcagcaaaag ggttatcaaa tgacatctga agaatccatc attcgttttg tcatgagaca
    15661 aacagagttt tcagaatcgc ttgtccgtag tttgctgaat cacttagggt ttgctcagga
    15721 aactttgacg aaaccgttat gtacattaag tgggggagaa gcgacccgtc tgacgattgc
    15781 tttgcttttt actaagccaa gtaatgtgtt gctgttagat gaaccgacta attttattga
    15841 tatggcaacg atcgaagctt tagagaagct aatgcaaata tatccgggaa cgattttgtt
    15901 tacttctcat gattcttact ttgtcgagcg tacggctgat gaagtttatg aaataaaagg
    15961 gcagaaaata aaaaaagtac ttacgagaaa tttttaaatg tgctacgatg attttcgtga
    16021 caaatttaaa ggagtggtac agattgccta caaataaaaa agaaggaata atttttacaa
    16081 caatgatgtg ttttttgatg gtcttgggga tgagcgcgta taatttatgg ctgcatcaag
    16141 atcttcattt atcaagtttg ttgattggtc tagtgcctgg attcgtggtg gctttcattt
    16201 tggatgtctt catcatcggt gtgctggcga aaaaaattgc ctttaaacta ccagtaaatc
    16261 cagaaagcaa ggtccaaatg atcttaacta tttcatgctt gatggtttta ggaatggtga
    16321 catgtatgtc tttattcggt gtgatcatgg aacaaggaat acctgatcaa ttattctctg
    16381 cttatatgaa tgcttggaaa atgaatgtga ttgccgcttt accgttgcag ttgatcatcg
    16441 ttggcccgct ttcaagaagt gtgctaaaag caattcaaag taatgaatga tggaacactg
    16501 ttatttgaaa aaaggccttg gcatgctttt tgtcatagtc tcttgaatgc agttctagaa
    16561 aaatagtgat ccgaacgata ggttttggcc tgtcgttcgg atcgcttttt cttttccaac
    16621 tgtattgctc ttttattgaa gataatttgc tcttcaatag atatttctag aaaggttctg
    16681 tttatccctt actcaaaaaa ctgctgcatt tgttctctta cttgctgaca ccattggagt
    16741 tcctgctgac aaagcataat tctgcgctgg atcacaacat aacgtccaaa ttcttgtttc
    16801 ttgtctagcg agaggtcatt caattttttt tgattttttt gcatgatttt gttcagctgt
    16861 tgttctctag tgaatagcag tccagtagct gttgttttat cgagcgtcga aaaagcatag
    16921 atttttgcta gaaattcatc tttttgcgta ggagcgggag tttcctcttt gatccattcc
    16981 tctacaagag cacgtccttc atcagtcaag tggtagatct ttttttgaga gtgctcgtct
    17041 ttgtccaaaa cggtcacata accctgctgg tctaacttgg caagcgttgt atagatctga
    17101 ctgtgatggg cttcccaaaa aattgtgatc agctgcttca attcataccc agaacgtggt
    17161 ttggataaaa gcacgcataa taatatatag gctaaagtat tcataaaaat cctacctctc
    17221 gtcttatttt ctttattata tcacagtaaa gtttccacgt tttacatgtg catatttaca
    17281 tgtaaaataa atcatgtatg atgctaatag agagaaaggg atggaaatat tggagaatca
    17341 aatagatata caagaacaaa caaatgaaga aacaaagggg aaaagttcag cgctagttgt
    17401 agcagctacc tgtatgatcg cttttatggg agtgggtcta gtagacccaa tcttaaaaac
    17461 gattgctgca cagttaaacg ctacgccagc tgaaacgacc ctgttattta ctagttacat
    17521 gttagtcact ggtgtggtga tgctatttac aggtttctta tctagtcgga tcggtttgaa
    17581 aaaacattga tgacaggatt gtttatcatc gttgtattcg ctggtcttgg cggattatct
    17641 gggaatatcg gaatgctgat tgggttacgt gccggctggg gtgtcgggaa tgctttattt
    17701 gtctctactg ctttagcaac gatcgtcagt attttagttg gtaacacaga aaaagcaatc
    17761 atgatgtatg aagcagcact tggctgcgga atggctgttg gccctctagt cggtggggtc
    17821 ttgggcagtg gttcatggcg ttatccattt tttggggtag catttttgat gttttgcggt
    17881 tttatagccc tagcggtact tttacctgaa actgagaaac caaagagaaa agtaggcctt
    17941 tgggaaggtg tcaaagcgtt aagcaatcat aaacttcgtt cagttggact aatcgcattg
    18001 ttatataatt tcggattctt tactttactt gcgtatgcgc catttttatt gattggctat
    18061 tctgaattag aagtcggttt tgtatttttt ggctggggtg tgcttctggc tattgcttcg
    18121 attttcgttg caccggctct tgagcggaag tttacgactt atcggacaat tttagcagca
    18181 tttgtcttat tcattatttg tttgctgtta ttaggagctg gaacttctat gccagtcctt
    18241 gtaccgatca gcattgtgtt agcaggattt ttccaaggaa ttatcaatac actgttgacg
    18301 acgattgcta tggaaatacc aggtctgcaa cgaaacgttg cttcgtccag ctatagtttt
    18361 gtccgctttt tcggtggagc tttagcacca tttattgcag gtgtgattgg tgaacatatt
    18421 ggtgaaagct atgcgtttta ttttgcaggt gccattgttc ttatcagtgt atgttgtatc
    18481 atttatcacc gtaattattt tgctacaaat gaagaagaat aagaaatcag aggttgtgaa
    18541 aaagctgtcc tgcatcaagt cataagaa
//
LOCUS       DACEPD010000234        17033 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.guided.063, whole genome shotgun sequence.
ACCESSION   DACEPD010000234 DACEPD010000000
VERSION     DACEPD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.guided.063"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(134..577)
                     /locus_tag="IW797_002884"
     CDS             complement(134..577)
                     /locus_tag="IW797_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ1225597.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IW797_002885"
     CDS             complement(795..2135)
                     /locus_tag="IW797_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1225598.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IW797_002886"
     CDS             2404..3300
                     /locus_tag="IW797_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ1225599.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IW797_002887"
     CDS             complement(3450..4391)
                     /locus_tag="IW797_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ1225600.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="IW797_002888"
     CDS             4517..5638
                     /locus_tag="IW797_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ1225601.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /locus_tag="IW797_002889"
     CDS             5896..7398
                     /locus_tag="IW797_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ1225602.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="IW797_002890"
     CDS             7465..7992
                     /locus_tag="IW797_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ1225603.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="IW797_002891"
     CDS             8203..9264
                     /locus_tag="IW797_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ1225604.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="IW797_002892"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="IW797_002892"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ1225605.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="IW797_002893"
     CDS             10177..12159
                     /locus_tag="IW797_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ1225606.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IW797_002894"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IW797_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ1225607.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="IW797_002895"
     CDS             12881..14305
                     /locus_tag="IW797_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ1225608.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="IW797_002896"
     CDS             complement(14486..15259)
                     /locus_tag="IW797_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ1225609.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="IW797_002897"
     CDS             complement(15256..16161)
                     /locus_tag="IW797_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ1225610.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="IW797_002898"
     CDS             complement(16315..16635)
                     /locus_tag="IW797_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ1225611.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         5511 a         3101 c         3487 g         4934 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaaaaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcg
//
LOCUS       DACEPD010000235        11422 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.guided.086, whole genome shotgun sequence.
ACCESSION   DACEPD010000235 DACEPD010000000
VERSION     DACEPD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.guided.086"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            39..977
                     /locus_tag="IW797_002899"
                     /pseudo
     CDS             39..977
                     /locus_tag="IW797_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            980..3163
                     /locus_tag="IW797_002900"
     CDS             980..3163
                     /locus_tag="IW797_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ1225612.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3160..4161
                     /locus_tag="IW797_002901"
     CDS             3160..4161
                     /locus_tag="IW797_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ1225613.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4176..5090
                     /locus_tag="IW797_002902"
     CDS             4176..5090
                     /locus_tag="IW797_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ1225614.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5335..5451
                     /locus_tag="IW797_002903"
     CDS             5335..5451
                     /locus_tag="IW797_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ1225615.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5467..7386
                     /gene="tet(M)"
                     /locus_tag="IW797_002904"
     CDS             5467..7386
                     /gene="tet(M)"
                     /locus_tag="IW797_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ1225616.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7484..8857
                     /gene="tet(L)"
                     /locus_tag="IW797_002905"
     CDS             7484..8857
                     /gene="tet(L)"
                     /locus_tag="IW797_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ1225617.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9421..10683
                     /locus_tag="IW797_002906"
     CDS             9421..10683
                     /locus_tag="IW797_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ1225618.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10928..11410
                     /locus_tag="IW797_002907"
     CDS             10928..11410
                     /locus_tag="IW797_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ1225619.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNGSVAKF
                     "
BASE COUNT         3771 a         1907 c         2388 g         3356 t
ORIGIN      
        1 acttggagat aacatcaaaa agttacctat cgctataacg tggcagatgg aaagaaacct
       61 tgccagagat ttcccatgaa atcaatatcg tcaatctgac ttctgatgag aaaaacaaag
      121 gcttacttga cccttatgta attatgaaaa ataccaaaga ttctgaatca ctggctattg
      181 atattttgac attccttacg gggatttcct ctcgtgatgg ggaacgcttc ccaatcctta
      241 gaaaagccat tcgtgcagta accaatagtg aagtgcgagg gttgatgaaa gtgattgaag
      301 aattacgggt tgagaatacg ccactaagta ccagcatagc cgaccatatc gaaagtttta
      361 cagactatga ctttgcccat ctgcttttta gtgatggtta tgtagagcag tctatcagcc
      421 ttgaaaaaca actgaacatt atacaggttg ccgacttggt acttcctgac aaggaaactt
      481 cctttgagga atataccaca atggagttac tatcggtagc aatgctgatt gtcattagta
      541 cctttgcgtt ggactttatc catacagacc gaagtatttt caagattgta gacttagacg
      601 aagcatggag cttcttacag gtggcacaag ggaaaacact atctatgaag ctggttcgtg
      661 ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca aaatacagat gacctcttag
      721 atgaaaaact gaaaaataac ctcggcttaa aatttgcctt tcgttccact gaccttaacg
      781 agattaaaaa aacccttgcc ttttttggtg tagacccaga ggacgaaaac aatcagaagc
      841 gattgcgtga tttggaaaac gggcaatgcc ttatcagtga tttatatggt cgtgtcggtg
      901 tgatacagtt ccaccctgta tttgaagaac tgctccatgc ctttgatacc agaccacctg
      961 tgcgaaaaga ggtgtaaatg tgaaaccatc aatattaaac agaataaaat caaactggac
     1021 gctgaaacgt ctaggtaaag tggcaatgac agtggctttc acacttgtga ttgccatttt
     1081 tcttttagcc atgctgggaa cggtggttca agctgcgggc ttggtagatg atacggtcaa
     1141 tgtggcaaat gaatacagcc gatacccact tgaaaactat caactggatt tttatgtgga
     1201 taatagctgg ggctggctac cgtggaactg gtcggacgga attggaaaac aagtcatgta
     1261 tggactatat gccattacca attttatttg gacaatcagt ctttatgttt ccaatgcgac
     1321 gggttactta gtacaggaag cctattcctt agacttcatt tccgctacag cagattccat
     1381 tggcaagaat atgcagaccc ttgcaggggt tacgcctagt ggactatcaa cagagggttt
     1441 ctatgttgga ttccttttac tcttgatttt ggttcttggg gtttatgttg cctatacagg
     1501 actgataaag agagaaacca caaaggcaat tcatgccctc atgaattttg tgctggtttt
     1561 tatcctatcg gcttccttta ttgcctacgc tcccgactac attaaaaaaa tcaatgactt
     1621 ttcatcagac atcagcaacg ctagtttgtc actgggtaca aaaattgtca tgccccattc
     1681 agatagtcaa ggcaaggaca gtgtggactt gatacgtgac agcctatttt ccatacaggt
     1741 tcagcaaccg tggctactgc ttcaatataa cagttcagac attgaaagta ttggtattga
     1801 ccgtgtggaa agcctgctct caaccagtcc agattccaac aatggggaag acagagaaaa
     1861 aattgttgcg gaagaaattg aagatagaag caataccaat atgacgatta caaagacgat
     1921 taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt ccatatttgt
     1981 attcctatta acaggaatca tgattttctc gcaggtactt tttatcatct atgctatgtt
     2041 tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt caaaacgagc
     2101 cataacaaag ctctttaata ccattttgac acgagctgga atcacattga ttattacgac
     2161 agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt tctttttgat
     2221 tgcttttcta cagattgtga cctttgcagg aatctacttt aagctgggcg atttaatgag
     2281 tatgttttct ctacagagta acgattctca aagtgtggga agtcgtatga tgagaaaacc
     2341 tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac gctccgtgac
     2401 tgctttaggg gctgggtctg ccattgccag tgctacgggt aaaaaaggac agtcgggttc
     2461 gggaagttct gcgagtacac aagcagatca ctctcgacca aacgggcagg aaaaatcaac
     2521 actaggaaag cgtatcggtc aaaccatcgg tacagtagct gataccaaag acagaatggt
     2581 agatactgct ggtaatttga aagaacaggt taaggatttg ccgaccaatg caagatatgc
     2641 agtatatcaa ggaaaatcta aagtgacgga taatgtccat gatttaacca gaagtatttc
     2701 tcaaaccaaa gcggataaag ccagtggacg caagaaacag caggaacaaa gacggaaaac
     2761 cattgcggag cgtcgctctg aaatggaaca ggtcaaacag aaaaaacagc cagcttcttc
     2821 cgttcatgaa agaccagcca ctaagcaaga acaatctcat gatggacaga cttcaaggca
     2881 aacaactgta caggcttcct atagggaatc tcaacaagcc aaacaagagc gtccaacagt
     2941 taagtccgat tcttcaagtt taaaaacgga acgtcaaagt aatacagttc aagaaagaac
     3001 ggttcaaaag ccagtaactt caactgcacc aacagataga gcttcacaac gttcaatcac
     3061 aaaagaacgt cagtctactg ttcaaagagt accactacaa aatacaaaaa gtagaccacc
     3121 aatcaaaacc gccaccatta agaaaggcag taagaaacca tgaagctgaa aactttagtg
     3181 attggtggtt cgggattatt cttgatggtc ttctcactgc ttctgtttgt cgccatttta
     3241 ttttcagatg aacaagatgg cggtttttcc aacattcact atggcggtgt ggatgtttcc
     3301 gcagaagtgc tggctcataa gcctatggta gaaaaagttg ccaaagaata tggaattgaa
     3361 gaatacgtca atatacttct tgcgattata caggtggaat cgggtggtac agcagaagat
     3421 gttatgcagt cctcggaatc cctcggtctt ccacctaatt cattgagtac agaagaatcc
     3481 attaagcaag gtgtgaagta tttcagtgag ttattagcca gtagcgaaag gcttggtgta
     3541 gatttagaat cggttatcca gtcctacaat tatggtggtg gtttcttagg atatgtggct
     3601 aatcgtggga ataaatatac ctttgaactg gctcaaagtt tctcaaaaga gtattcgggt
     3661 gacgaaaaag tgtcttaccc caatcccata gccataccta tcaatggggg ctggcgatac
     3721 aattacggga atatgtttta tgtgcaactg gtaacgcagt atcttgtcac aacagagttt
     3781 gatgatgata cagtacaagc catcatggac gaagctctga aatttgaagg ctggaagtat
     3841 gtctatggcg gagcttcccc gactacctct tttgattgta gcggactgac acaatggacg
     3901 tatggaaaag ctggaatcaa cttaccacga acagcacaac agcaatatga tgtaacccgg
     3961 catatcccat tattggaagc aaaagccggc gatttggttt tctttcattc tacctataac
     4021 gctggctctt atattactca tatcgggatt tatttaggcg ataaccgtat gtttcatgca
     4081 ggcgacccta tcggttatgc cgacttaaca agctcttact ggcaacagca tttagtggga
     4141 gcaggacgaa tcaaacaata agaaaggacg attcaatgat gaaattcgga aaaaatcaga
     4201 atacagaaaa acagacacca aaggaaaaga aacctcgtgt ctataaggtc aatcctcgta
     4261 aaaaggttgt gattgtccta tgggtacttt taggacttag tttcagcttt gcgatattca
     4321 agcactttac agccattgat acccatacca ttcatgaaac caccatcata gaaaaggaat
     4381 atgtcgatac tcatcatgtg gaaaattttg tagagaactt tgcgaaagtc tactattcat
     4441 gggaactaaa cgacaagtcc attgataatc gcatagaaaa cctgaaagca tatctgacag
     4501 aggaacttca agctctgaat gtcgatacag ttcgtaaaga tattcctgta tcgtcttctg
     4561 taaaaggatt tcagatatgg acggtagaag cagatggcga caatcaattt gatgtaacct
     4621 acagtgtaga ccaactcatt acagaggggg aaaatacaaa gaccgtccac tctgcttata
     4681 tagtgagtgt ctatgtagac agtactggaa atatggtcct cattaagaat ccgaccatta
     4741 ctagcatacc aaagaaatca gactataaac caaaagctat tgaaagtgag ggtacggttg
     4801 attccattac aaccaatgaa atcaatgagt ttttaacgac gttcttcaag ctctatccta
     4861 cagcgacagc cagtgaactt tcctactatg tgaatgacgg gatattaaaa ccaatcggaa
     4921 aagagtacat ctttcaagaa ctggtaaatc ctatttacaa tcgtaaggat aatcaagtca
     4981 cggtatcgct gacagtggag tatatcgacc agcagaccaa agcaacgcag gtatctcaat
     5041 ttgatttggt acttgaaaag aacgggagta attggaagat tatagaataa caaatattgg
     5101 tacattatta cagctatttt gtaatcacgt actctctttg ataaaaaatt ggagattcct
     5161 ttacaaatat gctcttacgt gctattattt aagtatctat ttaaaaggag ttaataaata
     5221 tgcggcaagg tattcttaaa taaactgtca atttgatagt gggaacaaat aattggatgt
     5281 ccttttttag gagggcttag ttttttgcac ccagtttaag aataccttta tcatgtgatt
     5341 ctaaagtatc cggagaatat ctgtatgctt tgtatgccta tggttatgca taaaaatccc
     5401 agtgataaga gtatttatca ctgggatttt tatgcccttt tgggcttttg aatggaggaa
     5461 aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcggg aaaaactacc
     5521 ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag cgtggacaaa
     5581 ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac aattcagaca
     5641 ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac gccaggacat
     5701 atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc aattctactg
     5761 atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc acttaggaaa
     5821 atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat tgatttatca
     5881 acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa acagaaggta
     5941 gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg ggatacggta
     6001 atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt agaagcattg
     6061 gaactcgaac aagaggaaag cataagattt cagaattgct ccttgtaccc tgtttatcat
     6121 ggaagcgcaa aaagcaacat agggattgag cagcttatag aagtgataac gaataaattt
     6181 tattcatcaa catacagaaa gaagtctgaa ctttgcggaa atgtcttcaa aattgaatat
     6241 tcggaagaaa gacaacgtct tgcatatgta cgcctttatg gcggaatcct gcatttgcgg
     6301 gattcggtta gaatatcgga aaaggaaaaa ataaaaatta cagaaatgta tacttcaata
     6361 aatggtgaat tatgtaaaat tgataaggct tattccgggg aaattgttat tttgcaaaat
     6421 gagtttttga agctaaatag tgttcttgga gatacaaagc tattgccaca gagagagaga
     6481 attgaaaatc cgctccctct gctgcaaaca actgttgaac cgagcaaacc tcaacaaagg
     6541 gaaatgttac ttgatgcact tttagaaatc tccgacagtg acccgcttct acaatattat
     6601 gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca aatggaagtg
     6661 attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc
     6721 atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca
     6781 aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg
     6841 cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg
     6901 gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa
     6961 atctgtttta agtatggctt atactatagc cctgttagta ccccagcaga ttttcggatg
     7021 cttgctccta ttgtattgga acaagtctta aaaaaagctg gaacagaatt gttagagcca
     7081 tatcttagtt ttaaaattta tgcgccacag gaatatcttt cacgagcata caacgatgct
     7141 cctaaatatt gtgcgaacat cgtagacact caattgaaaa ataatgaggt cattcttagt
     7201 ggagaaatcc ctgctcggtg tattcaagaa tatcgtagtg atttaacttt ctttacaaat
     7261 ggacgtagtg tttgtttaac agagttaaaa gggtaccatg ttactaccgg tgaacctgtt
     7321 tgccagcccc gtcgtccaaa tagtcggata gataaagtac gatatatgtt caataaaata
     7381 acttagtgta ttttatgttg ttatataaat atggtttctt gttaaataag atgaaatatt
     7441 ttttaataaa gatttgaatt aaagtgtaaa ggaggaatta attatgaagt gtaatgaatg
     7501 taacagggtt aaatacaacc aaattttaat ttggctttgc gttttatctt tttttagcgt
     7561 attaaatgaa atggttttga acgtctcatt acctgatatt gcaaatgatt ttaataaacc
     7621 acctgcgagt acaaactggg tgaacacagc ctttatgtta accttttcca ttggaacagc
     7681 tgtatatgga aagctatctg atcaattagg catcaaaagg ttactcctat ttggaattat
     7741 aataaattgt ttcgggtcgg taattgggtt tgttggccat tctttctttt ccttacttat
     7801 tatggctcgt tttattcaag gggctggtgc agctgcattt ccagcactcg taatggttgt
     7861 agttgcgcgc tatattccaa aggaaaatag gggtaaagca tttggtctta ttggatcgat
     7921 agtagccatg ggagaaggag tcggtccagc gattggtgga atgatagccc attatattca
     7981 ttggtcctat cttctactca ttcctatgat aacaattatc actgttccgt ttcttatgaa
     8041 attattaaag aaagaagtaa ggataaaagg tcattttgat atcaaaggaa ttatactaat
     8101 gtctgtaggc attgtatttt ttatgttgtt tacaacatca tatagcattt cttttcttat
     8161 cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc aggaaagtaa cagatccttt
     8221 tgttgatccc ggattaggga aaaatatacc ttttatgatt ggagttcttt gtgggggaat
     8281 tatatttgga acagtagcag ggtttgtctc tatggttcct tatatgatga aagatgttca
     8341 ccagctaagt actgccgaaa tcggaagtgt aattattttc cctggaacaa tgagtgtcat
     8401 tattttcggc tacattggtg ggatacttgt tgatagaaga ggtcctttat acgtgttaaa
     8461 catcggagtt acatttcttt ctgttagctt tttaactgct tcctttcttt tagaaacaac
     8521 atcatggttc atgacaatta taatcgtatt tgttttaggt gggctttcgt tcaccaaaac
     8581 agttatatca acaattgttt caagtagctt gaaacagcag gaagctggtg ctggaatgag
     8641 tttgcttaac tttaccagct ttttatcaga gggaacaggt attgcaattg taggtggttt
     8701 attatccata cccttacttg atcaaaggtt gttacctatg gaagttgatc agtcaactta
     8761 tctgtatagt aatttgttat tacttttttc aggaatcatt gtcattagtt ggctggttac
     8821 cttgaatgta tataaacatt ctcaaaggga tttctaaatc gttaagggat caactttggg
     8881 agagagttca aaattgatcc tttttttata acaggaattc aaatcttttt gttccattaa
     8941 agggcgcgat tgctgaataa aagatacgag agacctctct tgtatctttt ttattttgag
     9001 tggttttgtc cgttacacta gaaaaccgaa agacaataaa aattttattc ttgctgagtc
     9061 tggctttcgg taagctagac aaaacggaca aaataaaaat tggcaagggt ttaaaggtgg
     9121 agattttttg agtgatcttc tcaaaaaata ctacctgtcc cttgctgatt tttaaacgag
     9181 cacgagagca aaacccccct ttgctgaggt ggcagagggc aggttttttt gtttcttttt
     9241 tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt tggtcggcac tgccgacagc
     9301 ctcgcagagc acacacttta tgaatataaa gtatagtgtg ttatacttta cttggaagtg
     9361 gttgccggaa agagcgaaaa tgcctcacat ttgtgccacc taaaaaggag cgatttacat
     9421 atgagttatg cagtttgtag aatgcaaaaa gtgaaatcag ctggactaaa aggcatgcaa
     9481 tttcataatc aaagagagcg aaaaagtaga acgaatgatg atattgacca tgagcgaaca
     9541 cgtgaaaatt atgatttgaa aaatgataaa aatattgatt acaacgaacg tgtcaaagaa
     9601 attattgaat cacaaaaaac aggtacaaga aaaacgagga aagatgctgt tcttgtaaat
     9661 gagttgctag taacatctga ccgagatttt tttgagcaac tggatccagg agaacaaaaa
     9721 cgattttttg aggaaagtta taaattattt tccgaacgat atggcaagca aaatattgct
     9781 tatgcaacag ttcataatga tgagcaaacc cctcacatgc atttaggtgt tgtgcctatg
     9841 cgtgatggaa aactgcaagg aaaaaatgtg tttaatcgtc aagaactgtt atggctacaa
     9901 gataaattcc ccgagcatat gaaaaaacag ggttttgagt tgaagcgtgg tgaacgtggc
     9961 tctgaccgta aacatattga gacagctaaa tttaaaaaac aaactttgga aaaagagatt
    10021 gattttctag aaaaaaattt agcagttaaa aaagatgaat ggactgctta tagcgataaa
    10081 gttaaatcag atttagaagt accagcgaaa cgacacatga aaagtgttga agtgccaacg
    10141 ggtgaaaagt ccatgtttgg tttgggaaaa gaaataatga aaacagaaaa gaaaccaacc
    10201 aaaaatgttg ttatatcgga gcgtgattat aaaaacttag tgactgctgc gagagataac
    10261 gataggttaa aacagcatgt tagaaatctc atgagtactg atatggcgag agaatataaa
    10321 aaattaagta aagaacatgg gcaagttaaa gaaaaatata gtggtcttgt agagcgattt
    10381 aatgaaaatg taaatgatta taatgagttg cttgaagaaa acaagtcttt aaagtctaaa
    10441 ataagcgatt taaagcgtga tgtgagttta atctatgaaa gcactaagga attccttaag
    10501 gaacgtacag acggcttaaa agcctttaaa aacgttttta aggggtttgt agacaaggta
    10561 aaggataaaa cagcacaatt ccaagaaaaa cacgatttag aacctaaaaa gaacgaattt
    10621 gaactaactc ataaccgaga ggtaaaaaaa gaacgaagtc gagatcaggg aatgagttta
    10681 taaaataaaa aaagcacctg aaaaggtgtc tttttttgat ggttttgaac ttgttctttc
    10741 ttatcttgat atatatagaa ataacgtcat ttttatttta gttgctgaaa ggtgcgttga
    10801 agtgttggta tgtatgtgtt ttaaagtatt gaaaaccctt aaaattggtt gcacagaaaa
    10861 accccatctg ttaaagttat aagtgaccaa acaaataact aaatagatgg gggtttcttt
    10921 taatattatg tgtcctaata gtagcattta ttcagatgaa aaatcaaggg ttttagtgga
    10981 caagacaaaa agtggaaaag tgagaccatg gagagaaaag aaaatcgcta atgttgatta
    11041 ctttgaactt ctgcatattc ttgaatttaa aaaggctgaa agagtaaaag attgtgctga
    11101 aatattagag tataaacaaa atcgtgaaac aggcgaaaga aagttgtatc gagtgtggtt
    11161 ttgtaaatcc aggctttgtc caatgtgcaa ctggaggaga gcaatgaaac atggcattca
    11221 gtcacaaaag gttgttgctg aagttattaa acaaaagcca acagttcgtt ggttgtttct
    11281 cacattaaca gttaaaaatg tttatgatgg cgaagaatta aataagagtt tgtcagatat
    11341 ggctcaagga tttcgccgaa tgatgcaata taaaaaaatt aataaaaatg gttctgttgc
    11401 aaagttttaa ataaagaata aa
//
LOCUS       DACEPD010000236         7959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.guided.107, whole genome shotgun sequence.
ACCESSION   DACEPD010000236 DACEPD010000000
VERSION     DACEPD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.guided.107"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            128..1564
                     /locus_tag="IW797_002908"
     CDS             128..1564
                     /locus_tag="IW797_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ1225620.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="IW797_002909"
     CDS             1753..4200
                     /locus_tag="IW797_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ1225621.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="IW797_002910"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="IW797_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ1225622.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="IW797_002911"
     CDS             complement(5690..6793)
                     /locus_tag="IW797_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ1225623.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="IW797_002912"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="IW797_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ1225624.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2206 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DACEPD010000237         7482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.guided.111, whole genome shotgun sequence.
ACCESSION   DACEPD010000237 DACEPD010000000
VERSION     DACEPD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.guided.111"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            109..192
                     /locus_tag="IW797_002913"
     CDS             109..192
                     /locus_tag="IW797_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAQ1225625.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="IW797_002914"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="IW797_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ1225626.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1224..1484
                     /locus_tag="IW797_002915"
                     /pseudo
     CDS             1224..1484
                     /locus_tag="IW797_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1647..2312
                     /locus_tag="IW797_002916"
                     /pseudo
     CDS             1647..2312
                     /locus_tag="IW797_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2348..2593
                     /locus_tag="IW797_002917"
     CDS             2348..2593
                     /locus_tag="IW797_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ1225627.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2696..3565
                     /locus_tag="IW797_002918"
     CDS             2696..3565
                     /locus_tag="IW797_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAQ1225628.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3546..4280
                     /locus_tag="IW797_002919"
     CDS             3546..4280
                     /locus_tag="IW797_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ1225629.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4313..5225
                     /gene="ant(6)"
                     /locus_tag="IW797_002920"
                     /pseudo
     CDS             4313..5225
                     /gene="ant(6)"
                     /locus_tag="IW797_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5222..5764
                     /gene="sat4"
                     /locus_tag="IW797_002921"
     CDS             5222..5764
                     /gene="sat4"
                     /locus_tag="IW797_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAQ1225630.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5857..6651
                     /locus_tag="IW797_002922"
                     /note="aph(3')-IIIa"
     CDS             5857..6651
                     /locus_tag="IW797_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAQ1225631.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6759..6947)
                     /locus_tag="IW797_002923"
                     /pseudo
     CDS             complement(6759..6947)
                     /locus_tag="IW797_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6927..7469
                     /locus_tag="IW797_002924"
     CDS             6927..7469
                     /locus_tag="IW797_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ1225632.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         2623 a         1131 c         1567 g         2161 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1201 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1261 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1321 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1381 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     1441 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg ggctgaccca
     1501 aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg gaaagacctc
     1561 tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat ataggtgatt
     1621 aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac caactttgtt
     1681 tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc attgtgcagt
     1741 ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg ggtagacccc
     1801 aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa ggggtttatt
     1861 cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac ttatctcaag
     1921 agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt atgaacgata
     1981 ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc ttaatcagtg
     2041 gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg
     2101 tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc cttgaaaact
     2161 accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta aaggatgaac
     2221 tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa aatcaattaa
     2281 ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt aataaggagt
     2341 ggacaacatg attgatgtaa atgagctatt ggaagaagct attagagaaa ctgaaaatct
     2401 aaatgatggc gaagtttttc ttgttaaaga tttattcaag ggatatgtgg ggaataggat
     2461 acccagaaag gatcgacttc tgcttggaac gttattttta aaccatgtta gcaaaatgaa
     2521 tggtaattta aaggctattg aaaagacttc atctaatcag cagaggtata aaaagacaat
     2581 tgataagttt taactatctt ttgtgaaatt aaaaaaatta taaatatgtt gagtagaagc
     2641 aaaagatttg gcttggaatt tttacagcag tgaatcgata aaaggagcat gcttgatggt
     2701 agataatatt attaaatcag tagcagagaa attatcctct ctgtcttata tagaaggtat
     2761 tgttttaggt ggttcacgtg caaggggcac ccatacagag gattcggata tagatatcgg
     2821 catctattac aattcagaat catttgacat aaatactatt aatcaattcg ctacaaagct
     2881 ggatgatgag catagaaata accttgttgt acctcccgga gcatggggtg attggattaa
     2941 tggcggcgga tggttagtca taaacgggta tcatgtggat ttaattttac gtgatattaa
     3001 acgtgtggaa caaataatga aagatacaga gcacggaatt gttactgcca attatcagac
     3061 tgggcatccc catggttata ttagtgcaat gtatcgagga gaattagcga ttagcaaaat
     3121 actatatgct aagaatgaaa gcttatgcga attaaaaaaa caggcagaaa cttatcccaa
     3181 tgctttgcag aaaagtttag ttaacttttt tatgtttgaa gcagggttct ctttaatgtt
     3241 tgtaaaagca aattcgggaa cagacgataa atattatatt gcgggtcatg tttttcgtat
     3301 agtttcatgt ttaaatcaag tgttatttgc atgtaataat gcttattgta tcaacgaaaa
     3361 gaaagctata aaactgcttg aaacttttga acataaacct gaaaaatata ccgagaaggt
     3421 aaatcatatt tttgaagtac tcggtatctc actttttgaa tgctacgaca tgaccgagaa
     3481 gctttataat gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag
     3541 ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga
     3601 gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac
     3661 ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat
     3721 atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg
     3781 aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag
     3841 atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg
     3901 tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt
     3961 tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata
     4021 acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga
     4081 cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa
     4141 atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga
     4201 acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat
     4261 cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc
     4321 agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac gaacgtattc
     4381 gaattgtgac ccttgagggg tcacgcgcaa atattaatat acctaaagat gaatttcagg
     4441 attatgatat tacatatttt gtaagtgata tagaaccgtt tatatctaat gatgactggc
     4501 ttaatcaatt tgggaatata ataatgatgc aaaagccgga ggatatggaa ttattcccac
     4561 ctgaagaaaa gggattttcc tatcttatgc tatttgatga ttacaataaa attgatctta
     4621 ccttattgcc cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc
     4681 taattgataa agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg
     4741 taagaaagcc aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac
     4801 cttatgttat taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc
     4861 agattgttcg ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct
     4921 ttaaattaag tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt
     4981 gggagaaact tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat
     5041 ttctatgcca tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg
     5101 cctatccgga gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca
     5161 ctggtaaaac cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac
     5221 agtgattaca gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt
     5281 tgaggtgata ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt
     5341 actctatgaa gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga
     5401 ggcagattgc cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga
     5461 cgataaatgc gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga
     5521 agatatcgcc gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat
     5581 atctatagaa tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa
     5641 taaccttata gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac
     5701 tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt
     5761 ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat
     5821 ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg
     5881 gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct
     5941 gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc
     6001 cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa
     6061 ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg
     6121 ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct
     6181 gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg
     6241 gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat
     6301 aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg
     6361 cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac
     6421 ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat
     6481 cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc
     6541 agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag
     6601 cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta gtacctagat
     6661 ttagatgtct aaaaagcttt aactacaagc tttttagaca tctaatcttt tctgaagtac
     6721 atccgcaact gtccatactc tgatgtttta tatcttttct aaaagttcgc tagatagagt
     6781 tctatattag agatgtaaag agttttggtg aagaggtttg tgagtatggc ttttataacc
     6841 agggtgcaat gagaagcact aacagcagct agcttaagaa cgcataggat atacttttta
     6901 tggaagtcac agaggaggaa taaaatttga acaggattaa cagagtaacc tctattctta
     6961 ttcagctgca atcaaaaaaa ataattcctg ccaaagaaat tgcacagcga tttaatataa
     7021 gcttaaggac agtttacaga gatatccgga cacttgaaga agccggaata ccaattggat
     7081 ctgaggccgg aaaaggatat tttcttgtag agggcttcct acttccgccg gtaatgttta
     7141 cagcggcgga agtgggtgca ttgattacag cagggaaatt tttaaattgc catggagatg
     7201 aatcatttat aaaggatttt gattcagcta tgtataaaat caaatctatt ttgaagcatg
     7261 gtgaaaaaaa ctatgcacag gagttggaga acagtattaa cgtgtacagc acatctggac
     7321 agaaaaatac attggcggat aacgttattg cagcaattca gactgcaatc tgcaataaaa
     7381 gggtaatatc aattcaatat cctgcatcag gagggcaaga accggaaagc agaatgatag
     7441 agccggttct gttgcaaagt tttaaataaa gaataaaatc cc
//
LOCUS       DACEPD010000238         6882 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.guided.120, whole genome shotgun sequence.
ACCESSION   DACEPD010000238 DACEPD010000000
VERSION     DACEPD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.guided.120"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(565..792)
                     /locus_tag="IW797_002925"
     CDS             complement(565..792)
                     /locus_tag="IW797_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1225633.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            complement(801..1274)
                     /locus_tag="IW797_002926"
     CDS             complement(801..1274)
                     /locus_tag="IW797_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ1225634.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            2504..3070
                     /gene="amaP"
                     /locus_tag="IW797_002927"
     CDS             2504..3070
                     /gene="amaP"
                     /locus_tag="IW797_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ1225635.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            3083..3271
                     /locus_tag="IW797_002928"
     CDS             3083..3271
                     /locus_tag="IW797_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ1225636.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            3284..3841
                     /locus_tag="IW797_002929"
     CDS             3284..3841
                     /locus_tag="IW797_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ1225637.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            3873..4115
                     /locus_tag="IW797_002930"
     CDS             3873..4115
                     /locus_tag="IW797_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ1225638.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            4456..5670
                     /locus_tag="IW797_002931"
     CDS             4456..5670
                     /locus_tag="IW797_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ1225639.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            5701..5919
                     /locus_tag="IW797_002932"
                     /pseudo
     CDS             5701..5919
                     /locus_tag="IW797_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6338..6811
                     /gene="dfrF"
                     /locus_tag="IW797_002933"
     CDS             6338..6811
                     /gene="dfrF"
                     /locus_tag="IW797_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAQ1225640.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
BASE COUNT         2383 a         1039 c         1341 g         2119 t
ORIGIN      
        1 ctcgagttac gagtttggaa cagcttttgt ctacagtctg tcattattat caaaataatg
       61 ttaaaaataa aaaagaattg tccatcactg ttattcaaat cattttatca aaactacata
      121 gtaaatgtta aaaagcaaca tgacataata tatagaaggc tttacagtat atatgggagc
      181 ttactaaaaa agaatggagt aagagttaac acaatgggcc attagctgaa gttttaagca
      241 cttcaaggta tgaaacagaa tggcctgtaa tactacatga ttatttagac tgctcaaact
      301 gctatcagtc tggtggaatg aataaagtat tatactaaaa gcagatccaa ctacaacgta
      361 acaggaaacc agttgtatca ggaaaagtga tagtacatgg agaagaaaag gccccctttg
      421 tatgagtcag tacatatctt atttatagat aaaacggtat tcaatcgaaa taaaaaatct
      481 gaataataga cgtaaataac ttctaaaagt ctcacacatc tatcattcag attatttttt
      541 atttactgtt tgttctactt tattttattc ttctggtata gtattcgagt catttttata
      601 ggtcaacaaa tcacctggtt gacactccaa aacttggcag attttttcga gagtggaaaa
      661 acggatggct tttgcttttc cattttttag aatagagatg tttgccatcg taatgcccac
      721 tttctcactc aattcagtca cactcatttt tttttgagct aatactacat ctaaattaac
      781 gataattgcc atgtcgcacc tcacactgtc aaatcatgat ctgtcttaag taaaatcgca
      841 tgttcaagca gtttttccag cacgcttgtt aatgtgtata ctgcaaaagg aatgaatacg
      901 acagcgattc ctaccaacat caaacctgga gcatcgtcta aatccgctac tttcataaat
      961 ttaggaagta agccaaaaaa ggcgaaacca ataaacaaaa tggttcgttc gatttttttt
     1021 agtatgttta agctttgagt ggaaaaagct tggttatctt cgattttttt caatagtttc
     1081 aataagaaat aagcaataac gatccctagg agaagggcaa agtaaatgac agaaacaaat
     1141 aatgtggtct gccaatcaaa attgaatcct ttttcgcttg tagcgatctg agtataaaaa
     1201 agtacgccga acaaaataaa tacggcactt atacctaaaa cgacaaattt taatagcaat
     1261 gttttaaatt tcatttttat ccctctttta tctttgatag ctaaaatata gcagaataat
     1321 tattgtaaaa caactatttt ttatcgaaaa acaataaaaa tatatcgttt ataaaaaatg
     1381 atagagtatg aaggtgtgga gtgcatttaa cagctttttt tgattgtaag aaacatttaa
     1441 aaccagttat tttagattct atgtgtgtct cttctataat gctagtttag atgcctggga
     1501 ttatattgga caatacagtt ggaggatttt ttattatcta gcgaagagaa gtgtaagtta
     1561 acagaactgt atgagaaaaa agacaactac gtagtagcta accgattcat taattttttc
     1621 aagttcatgt ttatttacgt atcagaattt ttcttattca atggtttggt gtaatcacca
     1681 cttatttgtc ctttattgct tatacaatgt tatacaaaag gaaaatcaag cattgacgca
     1741 tggggtggta ctatctagct gaaatagctt aataagcgca agtagatgca ttggattagt
     1801 ttataaatca aaagtgttag aggtattaag aagaagtaga aaaataaaat ggattgccga
     1861 ataaaaagta ccataaatcc taaaacatgt gccaacagat agatggcaca tgtttatttt
     1921 tttcgataat tttgtaacaa aaatacgaag atgaaaaaga atagagattc ccataatgta
     1981 gtagtccaac tgaattcttt actgatacca aataccatat ttcctagatt tgatacaatc
     2041 aaatagccaa tcgttatgat caatgaatag tacaatcttt tcatcatagg acatacctcg
     2101 tttttattta taagaatagt ataaaccaaa gaagtgaata agaaaaatga taatataaaa
     2161 ataaagtaag aacgcattaa agtaactaaa gttatttatt gtatttttta attctccgaa
     2221 aaaatttaat aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt
     2281 aatcttgaat aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa
     2341 gcaattgtta tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg
     2401 gtagcgcttt atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt
     2461 agagttattt acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa
     2521 aataatcggt gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat
     2581 caatgcgccg tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa
     2641 ttactacatg caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat
     2701 catcttgcta gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga
     2761 agatggcaaa ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca
     2821 cgaagaagag ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca
     2881 tatccatgtc aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt
     2941 gatgcaagat atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt
     3001 ggcagtcaca tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg
     3061 agtggaatag gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat
     3121 ctttgggggt cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac
     3181 attgttgatc ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc
     3241 aggaatagtt gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat
     3301 gaatacaaca aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg
     3361 tgaattgact tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt
     3421 agatggtcta ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa
     3481 cacagataac gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga
     3541 tctagatgtg atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga
     3601 attgatcagt actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa
     3661 tgttgcagac atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa
     3721 agtaacagaa gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa
     3781 atctgctgta ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata
     3841 agaagtaaga agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg
     3901 gcggtatcat tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca
     3961 tcgcaaacat cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt
     4021 ggggaccaac aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag
     4081 tattagtcgt ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg
     4141 aaatgaagaa caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt
     4201 tatatgaagt attctttatt ccttatgaaa aaatatcttt aaaaataaaa aaagacgaaa
     4261 ataaacggtt ttctataaaa agaagaccgt ttattttttg tctttatttg tgtgaaaaac
     4321 gaaaaagagc acaacacatc aagcttttat taattgtctg tcttttataa atgcaagtat
     4381 taattcaaaa aaacagaaga aaaaaacaac tacaccttct tttatactcg taatcgtaga
     4441 aaggaggcga aaaaaatgaa tacattacct atcaatacag gagatacagc attcatgatt
     4501 ttatgtacag caatggtttg cctgatgact cctggtctag ctttttttta cggcggatta
     4561 gcacggaaga ataatatact aacgatcatg tctcaatctt ttctctccgt tgggataaca
     4621 acgattatgt gggtatttgg tggattcgga ttggcctttg gacgggatat cggcggtgtt
     4681 attggaaacc cggcagattt ctttttgatg agacatgtta caagcttccc caacatgttt
     4741 catggtgcaa cgattccatt tttaatgttc tttctttacc aactgatgtt tatcgtgatt
     4801 accgttcctt tgatgacagg agcttttgca gggagattga atttaaaagg ctatattata
     4861 cttgttattt tttggaatct gctgatttat ttccctgttt gccactggat atggggtggc
     4921 ggatttttgg atcagatggg atttagagat ttcgctggtg gagcagtcat ccacacgaca
     4981 gctggattcg gctctttagc atgtatcctc acacttggtc aaagaaagat tcctttagga
     5041 gaaagaagtc agcatagcag tcttatggca gctgctatcg gtacaggact gctttggttt
     5101 ggctggttcg gttttaattc cggtggtgca cttcgagcag atgaacaagc agtcaatgca
     5161 tttggttcaa cattcatcgc tcttgcattt gcgatgatta cttggctgat cattgcaaaa
     5221 gtaaaaggaa atggctttga ttttgtcgac gtactaacag gatccgttgc tggattggca
     5281 gccattacgc cttgtgccgg ttatgtagaa gcaaaaagtg ccatgctgat tgggatcatt
     5341 gcgggtatcg tctgtcatgc tgctgttgat tttcgtaaaa agaaacaatg ggatgatgca
     5401 cttgatgtgt ggggtgttca tggcatgggc ggattcacag gtacgattct gatagggatc
     5461 tttgcatcta acagccattt actaacgaat ccaagcagct ggtacttctt agggattcaa
     5521 gtgattggtg tcatcattac tgccgtgtat gcctatgtgc tgacaacagt gattttaaaa
     5581 tcggcgatgc actttacaac gatcactact acaaaagaag aacaagaaga aggattggac
     5641 tttttcttct ttggcaacaa actcaattga aacaaattca aatagaaaag aggaagattc
     5701 atgcaacaaa gcaaaacgaa aattcctgca aaaaaattaa cgttctttgg ctttttggct
     5761 atgaccatct caatggtcgt ttcattatat gaatatccca cattcgcgac ctcaggcttc
     5821 agcttagtat ttttcttgtt attaggcggt ttgctatggt ttatccctgt ggcattgtgc
     5881 gcagcagaga tggcaacggt gaaaggatgg gaaaaaggtg tttcccaaac aaaaagatat
     5941 taagaaacag cccatgttta cctgcttgca aacaaaagaa tattgctact agactaagag
     6001 atactacata agtgtgttgg tatctctttt ttgttgccgt gaaacagcag actatttcat
     6061 ttctgcttga ttaccttaaa acttattagt ctatggtttg tcaagggctt gttgcgtgag
     6121 aaatgctgaa tatcaccctt tacaaaacat aggctgataa gtaacttttc aaaagcagaa
     6181 aagaaatggc aatgcttata acaatatgat ataatgcaga tgaagacaaa aaaggaattt
     6241 gtcgagctgg ttagttcgag ataattttga gtttgttaag atgtggtgca cttgatggaa
     6301 tcacaggtac gacatcatat tatggaggat gaaatagatg ataggtttga ttgttgcgag
     6361 gtcaaagaat aatgttatag gcaagaatgg taatatacca tggaaaataa agggagaaca
     6421 aaagcaattt agagagttaa caacgggtaa tgtggttatt atggggcgaa agtcttatga
     6481 agaaatcggt catccgttgc ctaatagaat gaatattgtt gtttccacca caacagagta
     6541 tcaaggagat aatttagttt cagttaaatc attagaagat gcattattat tggctaaagg
     6601 acgagatgta tacatatctg gtggatatgg actatttaag gaagctttgc aaatagtaga
     6661 taaaatgtat atcacagaag tagatttaaa tattgaagat ggagatacat tctttccaga
     6721 atttgatatc aatgattttg aagttttgat aggggaaaca cttggtgagg aagtgaaata
     6781 tacgagaaca ttttatgtaa ggaaaaaatg aattgagtaa attttggatt tagggtgttt
     6841 tcataaatat attgcttttt atgcatatat aaattgttgt aa
//
LOCUS       DACEPD010000239         4995 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.guided.137, whole genome shotgun sequence.
ACCESSION   DACEPD010000239 DACEPD010000000
VERSION     DACEPD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.guided.137"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            complement(30..2180)
                     /locus_tag="IW797_002934"
                     /pseudo
     CDS             complement(30..2180)
                     /locus_tag="IW797_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2326..2901
                     /locus_tag="IW797_002935"
     CDS             2326..2901
                     /locus_tag="IW797_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1225641.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3115..3810
                     /gene="vanR"
                     /locus_tag="IW797_002936"
     CDS             3115..3810
                     /gene="vanR"
                     /locus_tag="IW797_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ1225642.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3788..4942
                     /locus_tag="IW797_002937"
     CDS             3788..4942
                     /locus_tag="IW797_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ1225643.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1560 a          939 c          967 g         1529 t
ORIGIN      
        1 aattttcttc tttcagcttt ttatttccca cttgtaaatc ggtagatggt ggctcctttt
       61 ccagttccat aatgtggatt tgcatctgca tgtagtgatg aaacacctag ctgcattctc
      121 ataccatctg acgaagatgt tgtaccgtcg ccccaataga aaggcaattg taatttatga
      181 tgaaagttta ctaatatggc ttgggcttta ttcatggcat cttcatacat gcgccattga
      241 gatacattgg ctagttgctt atatgtaagt ccgggtgtgg cttcggccat cttgctcaag
      301 ccaatattca ttcccattcc taaaagggca gccatgataa tgattgtttc ttccttatct
      361 ggttttcgat tattggaagc atgagtgaat tgctcatgaa atcctgttat atgggccaca
      421 tccatgagta aatcagttaa ttttattctt ggtagcatct gataaaggct tgcactaaat
      481 ttttttgctt cttctggaac atctttttct aagcgtgcaa gtgatagctt tcctttttca
      541 agagaaaccc catctaactt attggaattg gcagctaacc actttaacct ttcattaaag
      601 ctgctggttc tctccgttat ataatcttcg aatgataaac taactgataa tctcgtattc
      661 cccttcgatt gattccatgt atcttccgaa aacaaatatt cctcaaaatc cctatattgt
      721 ctgctgccaa caatggaaac atctcctgcc cgaacatgct cccgaagttc tgttaaaaca
      781 gccatttcat agtaatgacg attaattgtt gtaccatcat cctcgtataa atgtcttttc
      841 catcgttttg aaataaaatc cacaggtgag tcatcaggca cttttcgctt tccagattcg
      901 ttcattcctc ggataatctc aacagcttgt aaaagtggct catttgcctt tgtagaatga
      961 aattccaata ctcttaatag cgttggcgta tattttctta gtgaataaaa ccgtttttgc
     1021 agtaagtcta aataatcata gtcggcagga cgtgcaagtt cctgagcctc ttctactgaa
     1081 gagacaaagg tattccattc aataaccgat tctaaaacct taaaaacgtc taatttttcc
     1141 tctcttgctt taattaatgc ttgtccgatg ttcgtaaagt gtataacttt ctcatttagc
     1201 tttttaccgt tttgtttctg gatttcctct tgagccttac gaccttttga taacaaacta
     1261 agtatttgcc tatcatgaat ttcaaacgct ttatccgtta gctcctgagt aagttgtaat
     1321 aaatagatgg ttaatatcga ataacgttta ttttcttgaa agtcacggaa tgcatacggc
     1381 tcgtatcttg agcctaagcg agacagctgc aacaggcggt tacggtgcaa atgactaatt
     1441 tgcactgttt ctaaatccat tcctcgtatg tattcgagtc gttctattat ttttagaaaa
     1501 gtttcgggtg aaggatgacc cggtggctct tttaaccaac ccaatatcgt tttattggat
     1561 tcggatggat gctgcgaggt aataatccct tcaagctttt ctttttgctc atttgttaga
     1621 gatttactaa ccgtattaaa tagcttcttt tcagccattg cccttgcttc ccacaccatt
     1681 ctttcaagtg tagtgatagc aggcagtata attttgtttt ttcttagaaa atctatgcat
     1741 tcatgcagta gatgaatggc atcaccattt tccaaagcta attgatgaag gtacttaaat
     1801 gtcattcgat attcactcag ggtaaaagtt acaaagtcgt attcacttcg aatttctttc
     1861 aaatgatccc aaagtgtatt ttccctttga ggataatgat caagcgagga tggactaaca
     1921 ccaatctgtt tcgatatata ttgtatgacc gaatctggga tgcttttgat atgagtgtat
     1981 ggccaaccgg gataccgaag aacagctaat tgaacagcaa atcctaaacg gttttcttcc
     2041 ctccttcgct tattaactat ttctaaatcc cgtttggaaa aagtgaagta ggtccccagt
     2101 atccattcat cttcagggat ttgcataaaa gcctgtctct gttccggtgt aagcaattct
     2161 ctacctctcg caattttcat tcagtatcat tccatttctg tattttcaat ttattagttc
     2221 aattatatat caatagagtg tactctattg atacaaatgt agtagactga taaaatcata
     2281 gttaagagcg tctcataaga cttgtctcaa aaatgaggtg atattttgcg gaaaatcggt
     2341 tatattcgtg tcagttcgac taaccagaat ccttcaagac aatttcagca gttgaacgag
     2401 atcggaatgg atattatata tgaagagaaa gtttcaggag caacaaagga tcgcgagcaa
     2461 cttcaaaaag tgttagacga tttacaggaa gatgacatca tttatgttac agacttaact
     2521 cgaatcactc gtagtacaca agatctattt gaattaatcg ataacatacg agataaaaag
     2581 gcaagtttaa aatcactaaa agatacatgg cttgatttat cagaagataa tccatacagc
     2641 caattcttaa ttactgtaat ggctggtgtt aaccaattag agcgagatct tattcggatg
     2701 agacaacgtg aagggattga attggctaag aaagaaggaa agtttaaagg tcgattaaag
     2761 aagtatcata aaaatcacgc aggaatgaat tatgcggtaa agctatataa agaaggaaat
     2821 atgactgtaa atcaaatttg tgaaattact aatgtatcta gggcttcatt atacaggaaa
     2881 ttatcagaag tgaataatta gccattctgt attccgctaa tgggcaatat ttttaaagaa
     2941 gaaaaggaaa ctataaaata ttaacagcct cctagcgatg ccgaaaagcc ctttgataaa
     3001 aaaagaatca tcatcttaag aaattcttag tcatttatta tgtaaatgct tataaattcg
     3061 gccctataat ctgataaatt attaagggca aacttatgtg aaagggtgat aactatgagc
     3121 gataaaatac ttattgtgga tgatgaacat gaaattgccg atttggttga attatactta
     3181 aaaaacgaga attatacggt tttcaaatac tataccgcca aagaagcatt ggaatgtata
     3241 gacaagtctg agattgacct tgccatattg gacatcatgc ttcccggcac aagcggcctt
     3301 actatctgtc aaaaaataag ggacaagcac acctatccga ttatcatgct gaccgggaaa
     3361 gatacagagg tagataaaat tacagggtta acaatcggcg cggatgatta tataacgaag
     3421 ccctttcgcc cactggagtt aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa
     3481 ttcagtggag taaaggagca gaacgaaaat gttatcgtcc actccggcct tgtcattaat
     3541 gttaacaccc atgagtgtta tctgaacgag aagcagttat cccttactcc caccgagttt
     3601 tcaatactgc gaatcctctg tgaaaacaag gggaatgtgg ttagctccga gctgctattt
     3661 catgagatat ggggcgacga atatttcagc aagagcaaca acaccatcac cgtgcatatc
     3721 cggcatttgc gcgaaaaaat gaacgacacc attgataatc cgaaatatat aaaaacggta
     3781 tggggggttg gttataaaat tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa
     3841 actttacatg tatatcgttg caattgttgt ggtagcaatt gtattcgtgt tgtatattcg
     3901 ttcaatgatc cgagggaaac ttggggattg gatcttaagt attttggaaa acaaatatga
     3961 cttaaatcac ctggacgcga tgaaattata tcaatattcc atacggaaca atatagatat
     4021 ctttatttat gtggcgattg tcattagtat tcttattcta tgtcgcgtca tgctttcaaa
     4081 attcgcaaaa tactttgacg agataaatac cggcattgat gtacttattc agaacgaaga
     4141 taaacaaatt gagctttctg cggaaatgga tgttatggaa caaaagctca acacattaaa
     4201 acggactctg gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt
     4261 tgttatgtac ttggcgcacg atattaaaac gccccttaca tccattatcg gttatttgag
     4321 cctgcttgac gaggctccag acatgccggt agatcaaaag gcaaagtatg tgcatatcac
     4381 gttggacaaa gcgtatcgac tcgaacagct aatcgacgag ttttttgaga ttacacggta
     4441 taacctacaa acgataacgc taacaaaaac gcacatagac ctatactata tgctggtgca
     4501 gatgaccgat gaattttatc ctcagctttc cgcacatgga aaacaggcgg ttattcacgc
     4561 ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct ttaacaacat
     4621 tttgaaaaac gccgctgcat acagtgagga taacagcatc attgacatta ccgcgggcct
     4681 ctccggggat gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa aagataagct
     4741 agctgccata tttgaaaagt tctataggct ggacaatgct cgttcttccg atacgggtgg
     4801 cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag ggcagattta
     4861 cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga tgccagactt
     4921 ggttgataaa aggaggtcct aagagatgta tataattttg gctctttgtc aaatgatgtt
     4981 ggtaaagaga aatct
//
LOCUS       DACEPD010000240         4853 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.guided.138, whole genome shotgun sequence.
ACCESSION   DACEPD010000240 DACEPD010000000
VERSION     DACEPD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.guided.138"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            352..1263
                     /locus_tag="IW797_002938"
     CDS             352..1263
                     /locus_tag="IW797_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ1225644.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1416..1901
                     /gene="vanZ"
                     /locus_tag="IW797_002939"
     CDS             1416..1901
                     /gene="vanZ"
                     /locus_tag="IW797_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ1225645.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2188..2517)
                     /locus_tag="IW797_002940"
     CDS             complement(2188..2517)
                     /locus_tag="IW797_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ1225646.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2822..3151
                     /locus_tag="IW797_002941"
     CDS             2822..3151
                     /locus_tag="IW797_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ1225647.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            3141..3428
                     /locus_tag="IW797_002942"
     CDS             3141..3428
                     /locus_tag="IW797_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ1225648.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3707..4270)
                     /locus_tag="IW797_002943"
     CDS             complement(3707..4270)
                     /locus_tag="IW797_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ1225649.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         1699 a          735 c          834 g         1585 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccgg cagagcgata ttgcggtcat
       61 tatctgcgtg cgctgcggca agatagcctg ataataagac tgatcgcata gaggggtggt
      121 atttcacacc gcccattgtc aacaggcagt tcagcctcgt taaattcagc atgggtatca
      181 cttatgaaaa ttcatctaca ttggtgataa tagtaaatcc agtagggcga aataattgac
      241 tgtaatttac ggggcaaaac ggcacaatct caaacgagat tgtgccgttt aaggggaaga
      301 ttctagaaat atttcatact tccaactata tagttaagga ggagactgaa aatgaagaag
      361 ttgttttttt tattgttatt gttattctta atatacttag gttatgacta cgttaatgaa
      421 gcactgtttt ctcaggaaaa agtcgaattt caaaattatg atcaaaatcc caaagaacat
      481 ttagaaaata gtgggacttc tgaaaatacc caagagaaaa caattacaga agaacaggtt
      541 tatcaaggaa atctgctatt aatcaatagt aaatatcctg ttcgccaaga aagtgtgaag
      601 tcagatatcg tgaatttatc taaacatgac gaattaataa atggatacgg gttgcttgat
      661 agtaatattt atatgtcaaa agaaatagca caaaaatttt cagagatggt caatgatgct
      721 gtaaagggtg gcgttagtca ttttattatt aatagtggct atcgagactt tgatgagcaa
      781 agtgtgcttt accaagaaat gggggctgag tatgccttac cagcaggtta tagtgagcat
      841 aattcaggtt tatcactaga tgtaggatca agcttgacga aaatggaacg agcccctgaa
      901 ggaaagtgga tagaagaaaa tgcttggaaa tacgggttca ttttacgtta tccagaggac
      961 aaaacagagt taacaggaat tcaatatgaa ccatggcata ttcgctatgt tggtttacca
     1021 catagtgcga ttatgaaaga aaagaatttc gttctcgagg aatatatgga ttacctaaaa
     1081 gaagaaaaaa ccatttctgt tagtgtaaat ggggaaaaat atgagatctt ttattatcct
     1141 gttactaaaa ataccaccat tcatgtgccg actaatcttc gttatgagat atcaggaaac
     1201 aatatagacg gtgtaattgt gacagtgttt cccggatcaa cacatactaa ttcaaggagg
     1261 taaggatggc ggaatgaaac caacgaaatt aatgaacagc attattgtac tagcactttt
     1321 ggggtaacgt tagcttttta atttaaaacc cacgttaact aggacattgc tatactaatg
     1381 atacaactta aacaaaagaa ttagaggaaa ttatattggg aaaaatatta tctagaggat
     1441 tgctagcttt atatttagtg acactaatct ggttagtgtt attcaaatta caatacaata
     1501 ttttatcagt atttaattat catcaaagaa gtcttaactt gactccattt actgctactg
     1561 ggaatttcag agagatgata gataatgtta taatctttat tccatttggc ttgcttttga
     1621 atgtcaattt taaagaaatc ggatttttac ctaagtttgc ttttgtactg gttttaagtc
     1681 ttacttttga aataattcaa tttatcttcg ctattggagc gacagacata acagatgtaa
     1741 ttacaaatac tgttggaggc tttcttggac tgaaattata tggtttaagc aataagcata
     1801 tgaatcaaaa aaaattagac agagttatta tttttgtagg tatacttttg ctcgtattat
     1861 tgctcgttta ccgtacccat ttaagaataa attacgtgta agatgtctaa atcaagcaat
     1921 ctgatctttc atacacataa agatattgaa tgaattggat tagatggaaa acgggatgtg
     1981 gggaaactcg cccgtaggtg tgaagtgagg ggaaaaccgg tgataaagta aaaagcttac
     2041 ctaacactat agtaacaaag aaagcccaat tatcaatttt agtgctgagg aattggtctc
     2101 tttaataaat ttccttaacg ttgtaaatcc gcattttcct gacggtaccc ccaaactaag
     2161 atttcataga tagattttct gataacttta aaaaacacga atagcggaaa ggatgtcttc
     2221 attcgctaaa gagtaataaa ctacttttcc atcttgacgt gattttgcca ttccatgttt
     2281 ttttaaaaaa cgtaaatggt gtgacgttgt agcgacacta gcagtaagta tttctgctaa
     2341 atcacagacg cacatttcct tataggtttc taaagcatag aaaattttaa ttctagaaaa
     2401 atcgctgaaa catttcccta acacaagtaa gtttgaaaaa tctttttgat ccaatttatt
     2461 ttttattcca tttacttttt ctttatgtac gacagtcact tggcaaactt ttttcattca
     2521 tctcacctca cattcaaacg attatttgaa tgaagtatac ctatttgatc actcttttgt
     2581 caatctacct taattccaat acactattat aagcaatttc atgtattgaa gtaaacttac
     2641 ttgcaagtgc gtttttgatt ccaagttaaa aacatattat tattctacat aatttcctaa
     2701 tcaaataaaa ctcctttgaa aattgttcta aaggagtttt attattttgt atttctacta
     2761 aaaatagcgt acaatgtaat tactaagtaa ttacgaaatg taaaataatg gaggaaataa
     2821 tatgattaca aaaacaagaa aacaaggaaa ttctattatg ttaacggtcc ctaaagaatt
     2881 cgatgtgccc aatggtgttg aagtagaagc taagcttgta gagaatggta tcctttatga
     2941 gtttgtagag cctaaaaaag aattttttga ttttagtgaa gatattttgg ctgatattct
     3001 ttcagaaggt tatagcaaac aagagatctt aaaagaattt agaaatagaa aaaatgcgtt
     3061 aacttctgca tttaaatcta ttgctgaaga tactgttgca aattctaaac cgatgacaaa
     3121 agaggagttg gcagcagaaa ttggcttatg agattctacc ttctaaacac gtaataaagt
     3181 atctaaaaaa attaaaagag aaaacattaa aagaacaatt tttaacgata atttatgatg
     3241 agatagcagt tcgtcctcat tcgggagaac aaaaaacggg tgatttatca ggaatttggg
     3301 ctattggatt caagtatgca ggtacgacat atcgcgttgc atatgaaatt aaagataatg
     3361 cagtgatacc tatcttacta tgtggaacgc atgaaaattt ttatgaacaa ttaaaaaaga
     3421 ttaggtaaga acccaatgtt tcaacttgag aggatagacg acactttaaa agataatttt
     3481 taacagctga gagttgtaat accctataat tttaaaccac taacctatat atttatgaaa
     3541 aatagaaaaa attaaaacgc attttaagcc tctttcaaat taaaagcttc atccttatgc
     3601 acaaagaggg cttaaaatgc gttattttat attgtttttt agtaataata aaaaaatcac
     3661 tctaattctc ccataaatat tatagattcc taaagattaa aatagattat tccctattgt
     3721 cttttaagta gcggtagagt gttgccttgc taataccagt catttccgta atttctttta
     3781 cgctataaac ctgactttta tacaatttta ctgctcgatc aacatctttc tggttcactc
     3841 gtggacggcc acctttacga cctctcgcac gtgcactttt caaaccttct ttggtacgtt
     3901 caacaattaa atcacgttcg aactgactaa atgcttggaa gacagtcatc attaagcgtc
     3961 cgtgaggcgt gctggtatcg aagttttctt tcaagctcac cagcttaaca tggtgttctt
     4021 caaaataggt aacaaggtca atcaaatcct ttgtactacg acccaatcga gaaaaacttt
     4081 ctacaattac cgtatctccc ggacgaattt tatcttttaa acgatttagt tggggacgaa
     4141 ccgctttggt tcccgtcatt ttctcagtta ggatttcatt acaattttgt tcattgagta
     4201 agtctaattg acgatttagt tcttgtgaac gtgtactcac tcgggcataa ccataaatgt
     4261 atccactcat gcttttcttc ctttcgtact ttaattctgg cttgattgta tcataaaagg
     4321 gagttataga tacatagtta atgcaacaag ttatgataca aaatatgacc tcttttattt
     4381 aataaaagag gtcatttcat tttgtctcat aatcgattgt ttttgatacg agtgtgtata
     4441 cgaaataaac aaaaaaatct taagtaaatt tctagatacc taatttaatc ctaactagtg
     4501 tatcctaaag ttaataacga gtttatcaca atgaaaatta taaaatgctc ccatattaga
     4561 tataacatgc aatatttgaa ttagaactac aaaatttatt cgttttataa agtaaataat
     4621 gttgagatac taaggaatag aatattataa ggaattgagc tgaatgtatt ggataaaaaa
     4681 agaataactt tattagagat ggactataaa aatgctcgta attttcttct gaagtctgaa
     4741 agctatttga atgctcagct accagaatat attagttttg acgaaacttt aaaatctgct
     4801 aaaaaagttt taacttcgcc gtttgtataa ttaagcgaga caaataaaaa agc
//
LOCUS       DACEPD010000241         3000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.guided.174, whole genome shotgun sequence.
ACCESSION   DACEPD010000241 DACEPD010000000
VERSION     DACEPD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.guided.174"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            246..1214
                     /locus_tag="IW797_002944"
     CDS             246..1214
                     /locus_tag="IW797_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ1225650.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1207..2238
                     /gene="vanA"
                     /locus_tag="IW797_002945"
     CDS             1207..2238
                     /gene="vanA"
                     /locus_tag="IW797_002945"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ1225651.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2244..2852
                     /locus_tag="IW797_002946"
     CDS             2244..2852
                     /locus_tag="IW797_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ1225652.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          894 a          574 c          723 g          809 t
ORIGIN      
        1 gatgctggtg tcatccacca acatcaaaaa gtatagagcc ataatttttt aggaaaatct
       61 caaggttatc tttacttttt cttaggaaat taacaattta atattaagaa acggctcgtt
      121 cttacacggt agacttaata ccgtaagaac gagccgtttt cgttcttcag agaaagattt
      181 gacaagatta ccattggcat ccccgtttta tttggtgcct ttcacagaaa gggttggtct
      241 taattatgaa taacatcggc attactgttt atggatgtga gcaggatgag gcagatgcat
      301 tccatgctct ttcgcctcgc tttggcgtta tggcaacgat aattaacgcc aacgtgtcgg
      361 aatccaacgc caaatccgcg cctttcaatc aatgtatcag tgtgggacat aaatcagaga
      421 tttccgcctc tattcttctt gcgctgaaga gagccggtgt gaaatatatt tctacccgaa
      481 gcatcggctg caatcatata gatacaactg ctgctaagag aatgggcatc actgtcgaca
      541 atgtggcgta ctcgccggat agcgttgccg attatactat gatgctaatt cttatggcag
      601 tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca tgatttcagg ttggacagcg
      661 accgtggcaa ggtactcagc gacatgacag ttggtgtggt gggaacgggc cagataggca
      721 aagcggttat tgagcggctg cgaggatttg gatgtaaagt gttggcttat agtcgcagcc
      781 gaagtataga ggtaaactat gtaccgtttg atgagttgct gcaaaatagc gatatcgtta
      841 cgcttcatgt gccgctcaat acggatacgc actatattat cagccacgaa caaatacaga
      901 gaatgaagca aggagcattt cttatcaata ctgggcgcgg tccacttgta gatacctatg
      961 agttggttaa agcattagaa aacgggaaac tgggcggtgc cgcattggat gtattggaag
     1021 gagaggaaga gtttttctac tctgattgca cccaaaaacc aattgataat caatttttac
     1081 ttaaacttca aagaatgcct aacgtgataa tcacaccgca tacggcctat tataccgagc
     1141 aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg tttggatttt gaaaggagac
     1201 aggagcatga atagaataaa agttgcaata ctgtttgggg gttgctcaga ggagcatgac
     1261 gtatcggtaa aatctgcaat agagatagcc gctaacatta ataaagaaaa atacgagccg
     1321 ttatacattg gaattacgaa atctggtgta tggaaaatgt gcgaaaaacc ttgcgcggaa
     1381 tgggaaaacg acaattgcta ttcagctgta ctctcgccgg ataaaaaaat gcacggatta
     1441 cttgttaaaa agaaccatga atatgaaatc aaccatgttg atgtagcatt ttcagctttg
     1501 catggcaagt caggtgaaga tggatccata caaggtctgt ttgaattgtc cggtatccct
     1561 tttgtaggct gcgatattca aagctcagca atttgtatgg acaaatcgtt gacatacatc
     1621 gttgcgaaaa atgctgggat agctactccc gccttttggg ttattaataa agatgatagg
     1681 ccggtggcag ctacgtttac ctatcctgtt tttgttaagc cggcgcgttc aggctcatcc
     1741 ttcggtgtga aaaaagtcaa tagcgcggac gaattggact acgcaattga atcggcaaga
     1801 caatatgaca gcaaaatctt aattgagcag gctgtttcgg gctgtgaggt cggttgtgcg
     1861 gtattgggaa acagtgccgc gttagctgtt ggcgaggtgg accaaatcag gctgcagtac
     1921 ggaatctttc gtattcatca ggaagtcgag ccggaaaaag gctctgaaaa cgcagttata
     1981 accgttcccg cagacctttc agcagaggag cgaggacgga tacaggaaac ggcaaaaaaa
     2041 atatataaag cgctcggctg tagaggtcta gcccgtgtgg atatgttttt acaagataac
     2101 ggccgcattg tactgaacga agtcaatact ctgcccggtt tcacgtcata cagtcgttat
     2161 ccccgtatga tggccgctgc aggtattgca cttcccgaac tgattgaccg cttgatcgta
     2221 ttagcgttaa aggggtgata agcatggaaa taggatttac ttttttagat gaaatagtac
     2281 acggtgttcg ttgggacgct aaatatgcca cttgggataa tttcaccgga aaaccggttg
     2341 acggttatga agtaaatcgc attgtaggga catacgagtt ggctgaatcg cttttgaagg
     2401 caaaagaact ggctgctacc caagggtacg gattgcttct atgggacggt taccgtccta
     2461 atcgtgctgt aaactgtttt atgcaatggg ctgcacagcc ggaaaataac ctgacaaagg
     2521 aaagttatta tcccaatatt gaccgaactg agatgatttc aaaaggatac gtggcttcaa
     2581 aatcaagcca tagccgcggc agtgccattg atcttacgct ttatcgatta gacacgggtg
     2641 agcttgtacc aatggggagc cgatttgatt ttatggatga acgctctcat catgcggcaa
     2701 atggaatatc atgcaatgaa gcgcaaaatc gcagacgttt gcgctccatc atggaaaaca
     2761 gtgggtttga agcatatagc ctcgaatggt ggcactatgt attaagagac gaaccatacc
     2821 ccaatagcta ttttgatttc cccgttaaat aaacttttaa ccgttgcacg gacaaactat
     2881 ataagctaac tctttcggca ggaaacccga cgtatgtaac tggttcttag ggaatttata
     2941 tatagtagat agtattgaag aggttctgtt gcaaagtttt aaataaagaa taaaatccct
//
LOCUS       DACEPD010000242          645 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E451
            SAMN07141509-rid9868343.guided.234, whole genome shotgun sequence.
ACCESSION   DACEPD010000242 DACEPD010000000
VERSION     DACEPD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN07141509
            Sequence Read Archive: SRR5811375
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000740705.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000740705.1
            Genome Coverage       :: 71x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/20/2020 04:20:16
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,947
            CDSs (total)                      :: 2,912
            Genes (coding)                    :: 2,843
            CDSs (with protein)               :: 2,843
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 32 of 69
            Pseudo Genes (incomplete)         :: 32 of 69
            Pseudo Genes (internal stop)      :: 15 of 69
            Pseudo Genes (multiple problems)  :: 9 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN07141509-rid9868343.guided.234"
                     /isolate="E451"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: New York, Valhalla"
                     /collection_date="May-2010"
                     /collected_by="Wang, Guiqing, New York Medical College,
                     Valhalla, New York"
     gene            <1..>645
                     /locus_tag="IW797_002947"
     CDS             <1..>645
                     /locus_tag="IW797_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ1225653.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKSSPDSCVKCYTM"
BASE COUNT          258 a           64 c          112 g          211 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagtca agtccagact cctgtgtaaa atgctataca atgtt
//
